LOCUS       DAFEHS010000001       127352 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFEHS010000001
VERSION     DAFEHS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 127352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 127352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..127352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.001"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(83..1561)
                     /locus_tag="LP327_000001"
     CDS             complement(83..1561)
                     /locus_tag="LP327_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1724157.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1859..2794)
                     /gene="murB"
                     /locus_tag="LP327_000002"
     CDS             complement(1859..2794)
                     /gene="murB"
                     /locus_tag="LP327_000002"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBL1724158.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2867..3787)
                     /locus_tag="LP327_000003"
     CDS             complement(2867..3787)
                     /locus_tag="LP327_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL1724159.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3900..4652
                     /gene="xth"
                     /locus_tag="LP327_000004"
     CDS             3900..4652
                     /gene="xth"
                     /locus_tag="LP327_000004"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBL1724160.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5516..6049
                     /locus_tag="LP327_000005"
     CDS             5516..6049
                     /locus_tag="LP327_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBL1724161.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6539..7063)
                     /locus_tag="LP327_000006"
     CDS             complement(6539..7063)
                     /locus_tag="LP327_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1724162.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7056..7529)
                     /gene="tsaE"
                     /locus_tag="LP327_000007"
     CDS             complement(7056..7529)
                     /gene="tsaE"
                     /locus_tag="LP327_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBL1724163.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7652..8632)
                     /gene="pta"
                     /locus_tag="LP327_000008"
     CDS             complement(7652..8632)
                     /gene="pta"
                     /locus_tag="LP327_000008"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBL1724164.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8785..9465)
                     /locus_tag="LP327_000009"
     CDS             complement(8785..9465)
                     /locus_tag="LP327_000009"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL1724165.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9591..10460
                     /locus_tag="LP327_000010"
     CDS             9591..10460
                     /locus_tag="LP327_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL1724166.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10486..11001
                     /locus_tag="LP327_000011"
     CDS             10486..11001
                     /locus_tag="LP327_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724167.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11048..11641)
                     /locus_tag="LP327_000012"
     CDS             complement(11048..11641)
                     /locus_tag="LP327_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1724168.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11666..11845)
                     /locus_tag="LP327_000013"
     CDS             complement(11666..11845)
                     /locus_tag="LP327_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724169.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11922..12431)
                     /locus_tag="LP327_000014"
     CDS             complement(11922..12431)
                     /locus_tag="LP327_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724170.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12527..12907)
                     /locus_tag="LP327_000015"
     CDS             complement(12527..12907)
                     /locus_tag="LP327_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL1724171.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13237..13986
                     /locus_tag="LP327_000016"
     CDS             13237..13986
                     /locus_tag="LP327_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBL1724172.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14170..15447)
                     /locus_tag="LP327_000017"
     CDS             complement(14170..15447)
                     /locus_tag="LP327_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724173.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15526..16170)
                     /gene="pcp"
                     /locus_tag="LP327_000018"
     CDS             complement(15526..16170)
                     /gene="pcp"
                     /locus_tag="LP327_000018"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL1724174.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16369..17313)
                     /locus_tag="LP327_000019"
     CDS             complement(16369..17313)
                     /locus_tag="LP327_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL1724175.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17315..18001)
                     /locus_tag="LP327_000020"
     CDS             complement(17315..18001)
                     /locus_tag="LP327_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL1724176.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18475..18795
                     /locus_tag="LP327_000021"
     CDS             18475..18795
                     /locus_tag="LP327_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL1724177.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18949..19854
                     /locus_tag="LP327_000022"
     CDS             18949..19854
                     /locus_tag="LP327_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1724178.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19851..20624
                     /locus_tag="LP327_000023"
     CDS             19851..20624
                     /locus_tag="LP327_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL1724179.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20805..22229)
                     /locus_tag="LP327_000024"
     CDS             complement(20805..22229)
                     /locus_tag="LP327_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1724180.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22440..22886
                     /gene="nrdI"
                     /locus_tag="LP327_000025"
     CDS             22440..22886
                     /gene="nrdI"
                     /locus_tag="LP327_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL1724181.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22951..24933)
                     /locus_tag="LP327_000026"
     CDS             complement(22951..24933)
                     /locus_tag="LP327_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL1724182.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDFAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24947..25843)
                     /gene="murQ"
                     /locus_tag="LP327_000027"
     CDS             complement(24947..25843)
                     /gene="murQ"
                     /locus_tag="LP327_000027"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL1724183.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25846..26906)
                     /locus_tag="LP327_000028"
                     /pseudo
     CDS             complement(25846..26906)
                     /locus_tag="LP327_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
     gene            complement(27117..27683)
                     /locus_tag="LP327_000029"
     CDS             complement(27117..27683)
                     /locus_tag="LP327_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL1724184.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(27711..29213)
                     /gene="eat(A)"
                     /locus_tag="LP327_000030"
     CDS             complement(27711..29213)
                     /gene="eat(A)"
                     /locus_tag="LP327_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL1724185.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29471..30592)
                     /locus_tag="LP327_000031"
     CDS             complement(29471..30592)
                     /locus_tag="LP327_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL1724186.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30718..31659
                     /locus_tag="LP327_000032"
     CDS             30718..31659
                     /locus_tag="LP327_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL1724187.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31809..32705)
                     /locus_tag="LP327_000033"
     CDS             complement(31809..32705)
                     /locus_tag="LP327_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL1724188.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            32974..34314
                     /locus_tag="LP327_000034"
     CDS             32974..34314
                     /locus_tag="LP327_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1724189.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            34532..34975
                     /locus_tag="LP327_000035"
     CDS             34532..34975
                     /locus_tag="LP327_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL1724190.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(35479..36897)
                     /gene="nhaC"
                     /locus_tag="LP327_000036"
     CDS             complement(35479..36897)
                     /gene="nhaC"
                     /locus_tag="LP327_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL1724191.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(37300..38523)
                     /locus_tag="LP327_000037"
     CDS             complement(37300..38523)
                     /locus_tag="LP327_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBL1724192.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(38554..39393)
                     /locus_tag="LP327_000038"
     CDS             complement(38554..39393)
                     /locus_tag="LP327_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL1724193.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(39410..40090)
                     /locus_tag="LP327_000039"
     CDS             complement(39410..40090)
                     /locus_tag="LP327_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1724194.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(40092..41165)
                     /locus_tag="LP327_000040"
     CDS             complement(40092..41165)
                     /locus_tag="LP327_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1724195.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(41221..41459)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(41502..41735)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(41812..42483)
                     /locus_tag="LP327_000041"
     CDS             complement(41812..42483)
                     /locus_tag="LP327_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL1724196.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(42570..45248)
                     /gene="mgtA"
                     /locus_tag="LP327_000042"
     CDS             complement(42570..45248)
                     /gene="mgtA"
                     /locus_tag="LP327_000042"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBL1724197.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            45907..46344
                     /gene="ndk"
                     /locus_tag="LP327_000043"
     CDS             45907..46344
                     /gene="ndk"
                     /locus_tag="LP327_000043"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBL1724198.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(46366..47232)
                     /locus_tag="LP327_000044"
     CDS             complement(46366..47232)
                     /locus_tag="LP327_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL1724199.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(47392..49485)
                     /locus_tag="LP327_000045"
     CDS             complement(47392..49485)
                     /locus_tag="LP327_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL1724200.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(50234..50857)
                     /locus_tag="LP327_000046"
     CDS             complement(50234..50857)
                     /locus_tag="LP327_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724201.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(51016..52413)
                     /locus_tag="LP327_000047"
     CDS             complement(51016..52413)
                     /locus_tag="LP327_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL1724202.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(52445..53416)
                     /locus_tag="LP327_000048"
     CDS             complement(52445..53416)
                     /locus_tag="LP327_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1724203.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            53790..54215
                     /locus_tag="LP327_000049"
     CDS             53790..54215
                     /locus_tag="LP327_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724204.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(54703..55404)
                     /locus_tag="LP327_000050"
     CDS             complement(54703..55404)
                     /locus_tag="LP327_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL1724205.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(55420..55860)
                     /locus_tag="LP327_000051"
     CDS             complement(55420..55860)
                     /locus_tag="LP327_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL1724206.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(55860..56435)
                     /locus_tag="LP327_000052"
     CDS             complement(55860..56435)
                     /locus_tag="LP327_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBL1724207.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(57283..57762)
                     /locus_tag="LP327_000053"
                     /pseudo
     CDS             complement(57283..57762)
                     /locus_tag="LP327_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(57921..58622)
                     /locus_tag="LP327_000054"
     CDS             complement(57921..58622)
                     /locus_tag="LP327_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL1724208.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(58730..60139)
                     /locus_tag="LP327_000055"
     CDS             complement(58730..60139)
                     /locus_tag="LP327_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBL1724209.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(60187..62052)
                     /locus_tag="LP327_000056"
     CDS             complement(60187..62052)
                     /locus_tag="LP327_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL1724210.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(62173..63024)
                     /locus_tag="LP327_000057"
     CDS             complement(62173..63024)
                     /locus_tag="LP327_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL1724211.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(63334..64047)
                     /locus_tag="LP327_000058"
     CDS             complement(63334..64047)
                     /locus_tag="LP327_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL1724212.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(64115..64441)
                     /locus_tag="LP327_000059"
     CDS             complement(64115..64441)
                     /locus_tag="LP327_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL1724213.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(64564..64791)
                     /locus_tag="LP327_000060"
     CDS             complement(64564..64791)
                     /locus_tag="LP327_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL1724214.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(65113..66366)
                     /locus_tag="LP327_000061"
     CDS             complement(65113..66366)
                     /locus_tag="LP327_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL1724215.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            66427..67167
                     /locus_tag="LP327_000062"
     CDS             66427..67167
                     /locus_tag="LP327_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1724216.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(67253..68011)
                     /locus_tag="LP327_000063"
     CDS             complement(67253..68011)
                     /locus_tag="LP327_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL1724217.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(68008..69036)
                     /locus_tag="LP327_000064"
                     /pseudo
     CDS             complement(68008..69036)
                     /locus_tag="LP327_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(69056..72223)
                     /locus_tag="LP327_000065"
     CDS             complement(69056..72223)
                     /locus_tag="LP327_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724218.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(72447..73331)
                     /locus_tag="LP327_000066"
     CDS             complement(72447..73331)
                     /locus_tag="LP327_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL1724219.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(73426..73863)
                     /locus_tag="LP327_000067"
     CDS             complement(73426..73863)
                     /locus_tag="LP327_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724220.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(74080..74991)
                     /locus_tag="LP327_000068"
     CDS             complement(74080..74991)
                     /locus_tag="LP327_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL1724221.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(75448..75876)
                     /locus_tag="LP327_000069"
     CDS             complement(75448..75876)
                     /locus_tag="LP327_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724222.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(75904..76542)
                     /locus_tag="LP327_000070"
     CDS             complement(75904..76542)
                     /locus_tag="LP327_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1724223.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(76546..78528)
                     /locus_tag="LP327_000071"
     CDS             complement(76546..78528)
                     /locus_tag="LP327_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL1724224.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            78707..79372
                     /locus_tag="LP327_000072"
     CDS             78707..79372
                     /locus_tag="LP327_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1724225.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(80441..81832)
                     /gene="sufB"
                     /locus_tag="LP327_000073"
     CDS             complement(80441..81832)
                     /gene="sufB"
                     /locus_tag="LP327_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBL1724226.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(81837..82307)
                     /locus_tag="LP327_000074"
     CDS             complement(81837..82307)
                     /locus_tag="LP327_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBL1724227.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPRCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(82294..83529)
                     /locus_tag="LP327_000075"
     CDS             complement(82294..83529)
                     /locus_tag="LP327_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL1724228.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHRAIDVQALDCDF
                     FAFSGHKMCGPTGIGALYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(83526..84812)
                     /gene="sufD"
                     /locus_tag="LP327_000076"
     CDS             complement(83526..84812)
                     /gene="sufD"
                     /locus_tag="LP327_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBL1724229.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSTVDQLGKNITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(84825..85598)
                     /gene="sufC"
                     /locus_tag="LP327_000077"
     CDS             complement(84825..85598)
                     /gene="sufC"
                     /locus_tag="LP327_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBL1724230.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFNGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(85795..86643)
                     /locus_tag="LP327_000078"
     CDS             complement(85795..86643)
                     /locus_tag="LP327_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL1724231.1"
                     /translation="MKKKIFGLAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDNIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(86667..87353)
                     /locus_tag="LP327_000079"
     CDS             complement(86667..87353)
                     /locus_tag="LP327_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1724232.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(87346..88380)
                     /locus_tag="LP327_000080"
     CDS             complement(87346..88380)
                     /locus_tag="LP327_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1724233.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(88443..88699)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(88775..89113)
                     /locus_tag="LP327_000081"
     CDS             complement(88775..89113)
                     /locus_tag="LP327_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBL1724234.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(89115..89471)
                     /locus_tag="LP327_000082"
     CDS             complement(89115..89471)
                     /locus_tag="LP327_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBL1724235.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(89477..90664)
                     /locus_tag="LP327_000083"
     CDS             complement(89477..90664)
                     /locus_tag="LP327_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL1724236.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYFYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVVGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(90855..91700)
                     /locus_tag="LP327_000084"
     CDS             complement(90855..91700)
                     /locus_tag="LP327_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL1724237.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YPLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            91934..92899
                     /locus_tag="LP327_000085"
     CDS             91934..92899
                     /locus_tag="LP327_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBL1724238.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            93399..94442
                     /locus_tag="LP327_000086"
     CDS             93399..94442
                     /locus_tag="LP327_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBL1724239.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(94504..94968)
                     /locus_tag="LP327_000087"
     CDS             complement(94504..94968)
                     /locus_tag="LP327_000087"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBL1724240.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            95213..96160
                     /locus_tag="LP327_000088"
     CDS             95213..96160
                     /locus_tag="LP327_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL1724241.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDVDSVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(96371..98443)
                     /locus_tag="LP327_000089"
     CDS             complement(96371..98443)
                     /locus_tag="LP327_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL1724242.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKEMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(98376..98753)
                     /locus_tag="LP327_000090"
     CDS             complement(98376..98753)
                     /locus_tag="LP327_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBL1724243.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(98882..99460)
                     /locus_tag="LP327_000091"
     CDS             complement(98882..99460)
                     /locus_tag="LP327_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL1724244.1"
                     /translation="MRGNMKMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELCSMIHGYL
                     SLRFLGYFTKESTIDPEDSYFWMINDFIATLP"
     gene            99592..100884
                     /locus_tag="LP327_000092"
     CDS             99592..100884
                     /locus_tag="LP327_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL1724245.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLAHQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNAAALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLMGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            complement(100944..102062)
                     /locus_tag="LP327_000093"
     CDS             complement(100944..102062)
                     /locus_tag="LP327_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBL1724246.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(102076..103497)
                     /gene="arcD"
                     /locus_tag="LP327_000094"
     CDS             complement(102076..103497)
                     /gene="arcD"
                     /locus_tag="LP327_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBL1724247.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFAIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVAFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(103743..104864)
                     /gene="mnmA"
                     /locus_tag="LP327_000095"
     CDS             complement(103743..104864)
                     /gene="mnmA"
                     /locus_tag="LP327_000095"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBL1724248.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNHAEVVFD
                     EPVRAITPGQAVVFYDEMECLGGGLIDHAYQETKVLQYV"
     gene            105268..105867
                     /locus_tag="LP327_000096"
     CDS             105268..105867
                     /locus_tag="LP327_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL1724249.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(106017..107290)
                     /gene="efmA"
                     /locus_tag="LP327_000097"
                     /pseudo
     CDS             complement(106017..107290)
                     /gene="efmA"
                     /locus_tag="LP327_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
     gene            complement(107561..107905)
                     /locus_tag="LP327_000098"
     CDS             complement(107561..107905)
                     /locus_tag="LP327_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBL1724250.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(108042..109187)
                     /locus_tag="LP327_000099"
     CDS             complement(108042..109187)
                     /locus_tag="LP327_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL1724251.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSCQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLEQLVRAVKK"
     gene            complement(109367..110341)
                     /locus_tag="LP327_000100"
     CDS             complement(109367..110341)
                     /locus_tag="LP327_000100"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL1724252.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(110421..111296)
                     /locus_tag="LP327_000101"
     CDS             complement(110421..111296)
                     /locus_tag="LP327_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBL1724253.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     ALIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKEWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            111426..113333
                     /locus_tag="LP327_000102"
     CDS             111426..113333
                     /locus_tag="LP327_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBL1724254.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(113873..117175)
                     /locus_tag="LP327_000103"
     CDS             complement(113873..117175)
                     /locus_tag="LP327_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBL1724255.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESCTYT
                     NAQEGFALMVRDAVPKDHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIKLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSFLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTVSKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNESPNESPNESPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLI
                     GVVVYVMKQRKK"
     gene            complement(117447..117992)
                     /locus_tag="LP327_000104"
     CDS             complement(117447..117992)
                     /locus_tag="LP327_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL1724256.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(118004..118696)
                     /locus_tag="LP327_000105"
     CDS             complement(118004..118696)
                     /locus_tag="LP327_000105"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBL1724257.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(118715..118999)
                     /locus_tag="LP327_000106"
     CDS             complement(118715..118999)
                     /locus_tag="LP327_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724258.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(119012..119575)
                     /locus_tag="LP327_000107"
     CDS             complement(119012..119575)
                     /locus_tag="LP327_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL1724259.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            119802..120452
                     /locus_tag="LP327_000108"
     CDS             119802..120452
                     /locus_tag="LP327_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBL1724260.1"
                     /translation="MIKRNWPWLLALVLLFLFGFSFVLGLLLIGVVLVLWGLFGSRRK
                     KGKQEEIPALTNELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAKLKAI
                     DLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIKNKQT
                     YEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQKD"
     gene            120576..121772
                     /locus_tag="LP327_000109"
     CDS             120576..121772
                     /locus_tag="LP327_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBL1724261.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(121873..123249)
                     /locus_tag="LP327_000110"
     CDS             complement(121873..123249)
                     /locus_tag="LP327_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1724262.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSFSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIISILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(123286..124152)
                     /locus_tag="LP327_000111"
     CDS             complement(123286..124152)
                     /locus_tag="LP327_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBL1724263.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(124241..125266)
                     /locus_tag="LP327_000112"
     CDS             complement(124241..125266)
                     /locus_tag="LP327_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL1724264.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYRLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(125284..125958)
                     /locus_tag="LP327_000113"
     CDS             complement(125284..125958)
                     /locus_tag="LP327_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1724265.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKICEVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKP
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(126139..127035)
                     /locus_tag="LP327_000114"
     CDS             complement(126139..127035)
                     /locus_tag="LP327_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL1724266.1"
                     /translation="MKKRTGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     RNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYWKFKRQKQLRKNNRS"
     gene            complement(127109..127234)
                     /locus_tag="LP327_000115"
     CDS             complement(127109..127234)
                     /locus_tag="LP327_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL1724267.1"
                     /translation="MKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKFKMKSEV"
BASE COUNT        36582 a        26679 c        22270 g        41821 t
ORIGIN      
        1 tagttatatc tagttacctt ttctttgaaa aaaacataaa tcagctaata aatatgtgag
       61 acaaagctgc catccataaa tactagctga ttttttcagt taaatgtcta tttttttctc
      121 tgatgatggt atttccaaaa tattcccagt catctggagt cggtggattt ttccaaatta
      181 tctgttcttt attaaatttt tcaagtaaac aatcagataa ataaatattc gtacgagaag
      241 tgattcgact ctcaatcaca atattgtaat ttttaaatcc tttcacttga gaaataatgt
      301 aatcggtata agcagatcct tgcgaaaaat caatacagac atgaatcggt gcttcaaaat
      361 atgataccgg acaaataccg accaataaaa agagataatc ataaaataag cgaatcatga
      421 aacgttcatc ttttcccaag aaatagcgtt tattctcttg tatgaccttt tggattgctg
      481 tactgaatat tggatacgtc tcgaagaaaa actgtagctg ctttttagac gtaaagctag
      541 tcatctcaaa gtcgaatatc caattttttc gattgacatg catcataccc atacgaagtt
      601 cttttcgtat atatagtctt tcttcttcag agatttcaat cgaaaatggt aattgatcaa
      661 tcaattccac tgacattttt tgactaatca tgtggatatc atgaaaatga gcttccgcaa
      721 gtccaaatgc gccatctaat tcaaattctt cttccgtgat caaaaacaac atgatataag
      781 aaatctcatc aatgatctgc tctttaggga tatgaagaaa agaaatatgt gataaatcct
      841 tgatgatctt cattccttct gagctcttaa agtattcatc tgaaaagaaa cgttttccga
      901 ttggttgatt cccataggcc ctcaacaaac taatagtggt tagcaaattg aatttgattc
      961 gttttgttgc agacagtttt aaattgaaca gatattcaaa atattgcacg atactctttg
     1021 cttcattcat gacgctttct ctaaatggca tagaaaaacc attaaagacc ccgaaatata
     1081 ccccaaataa tacgttccgt atgtttattt cttccccaac taatatgttt ttctttaact
     1141 ttatttgatg ctgttcaaga aaagagacta attctcttct ttttacatag gctcttgata
     1201 tgcttaaaaa atgattctct gcaaattttt cgatcgttac ttttgaatcc acgatctccg
     1261 ttaacagtcg agcaattttt gatcgttgaa agtagattct tcttaaattt ttgatatccg
     1321 tcaaattcag acgttcgaca tggatctctg cttcttctga aatttggatc tctggctttg
     1381 ttttgaattc agtaaattct ttatttaatt ctatgatata atttcttgtt ttaaatttag
     1441 acaaacctaa tgtttcatat aacttaggca gcactacata attctttttc tcaaattcta
     1501 aatatctagc tagcagcagt ttttctctat cttcctcttc tataaggtaa tccaacttca
     1561 tatcctacac ctcaaaaaat gcctcatacc aattacttga tatgcagatg aaagttcatc
     1621 ttttcaccta tactgatttt atgatggatt atgcttgttt tttcatcagt tattctcatt
     1681 ttttatcttt tttttgagaa tattgcaaaa aatggaaaaa tttatgagga aatattctat
     1741 attttttgtt ttattgtgtt aaaatgtaca agatttgcgc catttgcgta aacaaccaca
     1801 aaaaagagca ggacggccac cgtcctgctc ttttacttat tttaagttcc attacttatt
     1861 aattggctaa acttctgctt gtttctgata ttgcttgttt ttctccaatg atccgcactt
     1921 ctgtttctaa agacacatca aatttttctt tgattacttc ttggatgtgc gcaatcaatt
     1981 caatataatc tgtcgctgtc gcatgatcga tattgacgat gaatccagca tgtttttcag
     2041 agacttgtgc accgccccat ttcaggcctt gcaatcctgc ttgttggatc agctgacctg
     2101 tatagtgtcc tactgggcgc ttaaagacac ttccgcatga agggtattct aatggctgtt
     2161 ttgattgacg caattcggtc aactcatcca ttcttgcttt gattgcttca taatcccctg
     2221 tttgcaaact gaatcgagct tccaatacga tagcacgtaa ttcttggact ttactgtgac
     2281 gataagaaaa taccatttcg tcttttgtca aagttttcaa tgttccatct tcaagcaaaa
     2341 gatccacttc agcaaaaaca tctttgatct ctccgccata cgcacctgca ttcatataaa
     2401 ccgctccacc gacacttccc gggatgccgc aagcaaattc aaatccagtc aatgattctg
     2461 ccaaagctgc ataagtcgta tcaatcagtg aggcacctgc ttccgcaatg accatcgttc
     2521 ctttgacatg aatctgtttc atttcagtca gcatgatgac tacaccgcga atcccgccat
     2581 cttgaacgat cagattgctt gcatttccta ataccatcca aggtatatca tggattcggc
     2641 aataatcgat gatttgcttt acttcttctt gttttttcgg gaatgcaagg acatcagctg
     2701 gtccacctgt tttcgtaaac gtatagttca tcaatggttc atcaaacaat atagtaatcg
     2761 tggctaagct ttcaactatt tcttctttat tcactgagga ttttcctttc ttttctcaaa
     2821 cgtcctcatt attctatcac gcttaataca atcttgccag tcttgttcaa agttgatgac
     2881 ggtagtgatc tttaagcact tcctcacata attcatgact tcttacaact tctttttgtt
     2941 taagcaattc tgtttccgat ttacctaaac ggatctgaga aaagaaggcc tctaccattg
     3001 gctcaaaccc gcgtttgact agcgtgtttg tccagtcgcc aaattctttc actttttttc
     3061 catcagcatc ttgaatgaca agctgggtca gatcgtttac ttcataagta ccatttttac
     3121 ttgttacttg atatttttct atattagcgc cacttgctaa atccatcgtt aaaatagccg
     3181 tttgattttg tgtttctaat tgtaaaatgg catattccaa aaattccttt gtttctctta
     3241 tctggtattt tgttctcaaa atgggttcat ctaataaata cacagctgta tcaataagat
     3301 gtaaaaacaa atcataaagg acgaactctg tcgtttcttt tgccgctatt cgattttttt
     3361 gcaggtggat caaccgctta tctgggatac gtttcaattc ttctaccata ggagcaaagc
     3421 gccggttgaa accgatcatc aaaattattt ggttcttatc agctaattct tgcaattcac
     3481 gaacttcatt tagatggaca ctcagtggtt tgtcaatata aacatggata ccattttcta
     3541 gacatttttt ggctaattca taatgtactt ttgttgctgc gtggatcata catgcttcga
     3601 ttttttcttc taaaagctca tcgatcgttt catatagatg ttggaacccg tactcttttt
     3661 gaattctttc ttttgtttct ttatttcttg ttgcaaaata aaaatcaagt gttccttgtt
     3721 ttttcgcata agttggtaaa tatgcttttt ctgcgatatt ccctacaccg attacaccaa
     3781 tcttcatcta ctttcctcct ggatcattta gttactttca tcttacacca ttttttacta
     3841 aaatgaggta caatagtatc gcaaaggtaa ggaaattaat tcgaaggagt gtgcttttat
     3901 tgaaatttat ctcttggaac gtaaacgggc ttcgtgccat cgtaaataaa aattttttag
     3961 aagtttttca tgagttagac gccgattttt tctgtctgca ggaaacgaaa ctccaagcag
     4021 gacaaattga tttagatctt ccaggttatt atcaatattg gaattacgcg gaacgaaaag
     4081 gctattccgg cacagcgatt tttgctaaaa aaccagcatt gaatgccact tacggaatgg
     4141 gtatcgatat ccatgataca gaaggccgtc tgattacttt ggaatattcc gatttctttc
     4201 ttgtaacctg ttacacgcca aattctcaaa gcgaattaaa acgtttagat taccgattag
     4261 aatgggaaga agccttttat aattatttgg aaaatctaaa aaaacaaaaa ccagtcatcg
     4321 tgtgtgggga tctaaatgtc gctcatcaaa agattgattt gaaaaactgg aaaacgaatc
     4381 aaaaaaatgc tggattcact ccagaagaaa gagcggctct ttctcgactg ttggataacg
     4441 ggtttatcga tacattccgt tatttttatc caacccagga aggtgtctac tcttggtgga
     4501 actaccgatt caattcaagg aaaaacaatg ctggttggcg gatcgattac tttttgacga
     4561 gcaaagattt agagccagga ctagctgatg caaagatcca tacggacatt atgggcagtg
     4621 atcattgtcc agtagaatta gatttgaaat aaagaggttg tgaaaaagtc gtttagtttc
     4681 gagtattaag aaaaattttg tgaaaatggc tttccacatt ttgcataaat ttggcataat
     4741 accgagaaac tggcttttga acactgttta tcgagagatt gtgaaaaagc tgtcctgcat
     4801 caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttgggctta
     4861 ttaccgcaga tacaagcttt tgaacaccgt ttattaagag gttgtgaaaa agctgcactg
     4921 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc
     4981 ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtga aaaagctgca
     5041 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg
     5101 gacttattac cgcagatgca agcttttgaa caccgtttat tgagaagttg tgaaatcagc
     5161 gtcctgacct gagtattaga gaaacaaatg gcaaaatagt tcctcatatt ttgctatttt
     5221 ttgagctaat accgagggtc agctgattga acaccgttta gtggagaagt tgtgaaaaag
     5281 tcgcctactt tcgagtcgta agaaattttt ttgaaaatgg ttcctcacaa ctttgcataa
     5341 atttgactta ataccgagaa gtcatgatcc ccatctaact ccaaatcaca aggaatgagt
     5401 tcaaaaacta gcttttctca acaaatatta cataaaaaat tctatgtttt ttgttaaatt
     5461 tatttgcttt tttcaaaagt atgttacagt aatttcccaa ggaggtttta cagaaatgaa
     5521 ttttgttgcc atggattttg aaactgccaa tcatcagcct tatagtgctt gttctcttgc
     5581 gctagtcatg gtaaaaaata gccagatcgt tgatgaattc tatacattga tacagcctga
     5641 gactcctttt ttctggcgaa atgtccaaat tcacggaatc catcaagaag acgtacgcaa
     5701 tgcgccgaaa ttcccagaag tctggcaaac tatccaacca tattttcaaa aaaatcgttt
     5761 agtcgttgca cataatgctg cttttgatac aaaggtctta gcgggatgct tagattatta
     5821 tcatcttgaa cagcctaatt atctttcttt atgtacagtg aaaagtagcc gtcgcctgtt
     5881 tcctgaattt ccaaatcatc gattgaacac tgtgtgcgaa aatttgaaca tccaattaga
     5941 tcatcaccat gatgcattgg aagatagtcg tgcatgtgcg gaaatcctgc tttatcaaga
     6001 aaaaaaattc gggacagatc cattgaaaaa acttgtgact ataaagtaaa agaatactta
     6061 tgaaacacac aaagaggtcg tgagcctgcc gtttcactcc gagtcacaag gagcaatttc
     6121 aaaaaatagc ttctcatgtt tattaaatat tgtgactggt gaccgaggag ctggcaggag
     6181 aacaccgttt attggagagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc
     6241 aaacaaaaat agcttctcat atttttcatt tggttggact tattaccgca gatgcaagct
     6301 tttggacacc gtttaccaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa
     6361 caaacggcaa aatagttcct catattttgc cgttttttga gctaataccg aaggtcagct
     6421 gattgaacac cgtttattca aaaaaaatgg actgcgacac gacttttgtc acagtccctt
     6481 cgtgtgtttt tctgccttct caaaaggatg acagtttctc actcttttcc tatttcttct
     6541 attcaatcaa ataactcatt ttatatacat ctaaaaattc acctagatct gttcttgccc
     6601 ctctaggcat gaccgcttcg atttggaagc ccatttttcg gtaaaggtgt acggctcttt
     6661 cattacgaac ttgaacgtca agctccaatc gaaaaagtac acccatttcc ttcgcccaac
     6721 tgatgatttc ttctagcata agagtgccta gtcccattcc ccaatattcc tgcagaatac
     6781 tgataccgac ttctcccacg tgagacaaac gaaattgact gtctgccttg acagatgctg
     6841 ttccaataat cgtcccttca tagattgcaa gcagcaataa attattattg ctttcgtata
     6901 tataatccaa ctcttcttct aaggtttctg gtggtaacag gagttctgct tcatccaaaa
     6961 ctaaaaattc tgtctcctta ttaacttgtc ccatcatcaa aagaatgtct tttgcatctc
     7021 ctggcactgc ttcccgcaag atgatcccaa cttcttcact catgattttc agcccatttt
     7081 tctagaattc gctgtttgaa ggtttcactt tgctccccgt atgagattag tttcacatac
     7141 cgcttttcta aatctgtatc acttttttct aaaatcagtt ccaaataatc ctccggcagt
     7201 gcttcttcta atagatttcc ccattcaaca acggaaagcc catcaccttc aaaatattca
     7261 tctaacccta aatctgctcc gctagctgct acccgataaa tatccatatg atacaacgga
     7321 atccgacctt gactatattc tctaataatc gtgtatgtcg gacttttgat catttgcgtt
     7381 atccctaatc cttgggcaat tcctttcgtc aacgttgttt tacccgcacc tagatcacca
     7441 gtcaatatca gattgtcacc tggcattgcg gaatgaccaa ttgcctctgc gaattgttct
     7501 gtcattgtta atcctgatag ttcgatcatt tttcatcttc tcccgctctt ctttcttttt
     7561 gccagtatat cgcgtaatga agttttttta aagaaaaaat gaactagtca agaacagcag
     7621 ctgctgtttt tggctagttc gcgtgtattt gttaatcaat caatgtttgt gctgctgtga
     7681 taatcgataa tttatataca tcttcttcat ttgctccacg agacaaatca gaaaccggtt
     7741 tgttcaagcc ttgtagaatt ggtccgattg cttcaaagtt accgaaacgc tgagcaattt
     7801 tatagccgat atttcctgac tgcaattctg gaaatacaaa gactgttgct tgaccagcta
     7861 cttctgaatc tggcgctttc agttgtgcaa ctgaaggaac ataagaagca tcaaattgca
     7921 attcgccgtc gatcatgtat tgtggcgcta gttttttagc gattttagtt gcttcaacta
     7981 ctttgtctac ttctggcgat ttcgctgagc cttttgtcga gaagctcatc attgctactt
     8041 ttggttcgat atcgaacaat tctgctgttt ttgcagaatc tacggcgatt tcagccaatt
     8101 cttgcgcagt tggattcacg ttgatcgcac aatccgaaaa gacatatttt tcttgatctc
     8161 ttccacgtac catgatcatc gctccgcttg tccgactgat gccaggcttt gttttgatga
     8221 tctgaagtgc tgggcgaaca gtgtcgccag tcgaatggat cgctccagag accattccgt
     8281 ctgcaatccc catatacgta agcatcgtac caaagtaatt cacatctttt aaaatctttt
     8341 ctgcttgttc tttggatgcc tttccatttc tacgttcaac aaaagtatcc accatttcat
     8401 cccacttatc gtaattatca gggctgatga tcgtaaagct agatgttttg attcctctgg
     8461 catgcgctgc atttcttatt tcttcttcgt tccctactaa aatgggttcc acaagttctt
     8521 ctgctttcaa ccgggcagct gcgcctaaaa tacgagcatc agtagcttcc gggaaaacga
     8581 ttttgatccc acggcgaaca attttaaatc ttaaactatc aaacaattcc acaagatgac
     8641 ctccagacgt ttttatatgt attatcatac actattccag aaaaaaagaa cagtttattt
     8701 tatctgtatc agtcaaaaat gctggaaaaa ttaagttttt cacatacaaa gcgaactgca
     8761 ctataacagg aaagcgctgt tttattacac tgtttcaggc agttgccaat caatcggttc
     8821 ttctcctaac gcaaggagcg catcattcgt tttcgagaag ggtttggaac caaaaaagcc
     8881 acgacttgca gataacggac tgggatgtgg agatgttatg atgatatggc gactcttatc
     8941 gatcattttg attttttctt gtgccggttt tccccacaag ataaacacga ttggtttttc
     9001 ccgttcattt aatttttcaa tgatcgtgtc tgttaatctt tcccaacctt tcccgcgatg
     9061 agaatacgct tgcccttccc gaacggtaag aacggtgttg agtaaaagga ctccctgttt
     9121 agcccatgac actagatttc catgttgaac aggtgcgatt ccaagatcat cataaagctc
     9181 tttatagata tttcttaacg atggcgggat ctttacgcca ggttgcacag aaaaagataa
     9241 gccatgagct tgattttctc catgataagg atcttgtcct aaaatcacca ctttaacttc
     9301 ttcataagga gtcaattcca atgcttcata gatatgatac atatctggat ggatcttttg
     9361 ggacgcatat tcttttttta aaaattctct tagatgcaga taataatctt ttgaaaattc
     9421 tgaggacaaa acttcttgcc aactgttatg aatgatttct ttcaaaaact ccacctccta
     9481 ataaaaagaa taactcacat tttcttcaat cacaagggac aatcttcacc ctcagcactt
     9541 gattaatatg gtaaactagg aacaagaagc agaaaggaac gataatcatt atgatcaaat
     9601 taattgcatc agatatggac ggcacattat tagatgccca tatgagtgtt tctcctgaaa
     9661 atgccgaggc gatacgtttt gcaaatgatg ccggtgttga gttcatggtc gctactggtc
     9721 gaaactatca agaagctcgc gcagctttag atgaggtagg aatcgattgt gcgatgatca
     9781 ctttgaacgg ggcacaggta tttgataaag aaggaaattc attgtttacc gtgtcgattc
     9841 caaataatca agcaatctct gttttagata ttctagatgc taacggtatt tactatgaag
     9901 tagcaaccaa cgatggtctt tattctgaaa atcaagcgaa acgaattgaa agttttgctt
     9961 ccatggtggc tacccattta cctcacctta cgtataaaat ggcgattgcc atggcctcag
    10021 ctaagctgga gttattgcac atcacatacg tggacagtat tcgtgctatt ttggaagaaa
    10081 agaatctgga agttttaaaa attatttgtt ttcatacaga agggccagct gtattaggtc
    10141 cagttggaaa gcagatttct cttttaggcg atctagccgt tacttcctct ggtcaaaaca
    10201 atatcgaagt gaaccataaa aacgctcaaa aaggaattgc tgttgcccat gtcgctcacg
    10261 agcgtgggat ttctttagaa gaagtcatga cgattggcga taacttcaat gatgtcagca
    10321 tgctgcaagt agcaggagtc agtttcgcaa tgggaaatgc agaaatcgaa gtaaaagatt
    10381 atgccaaata tacaacggac acaaaccttg aatctggcgt aggaaaagcc atattgcgcg
    10441 cgattgcaga agacttgtag ttttatctag aaaagaggga tatagatgtc agaatttttc
    10501 attcaggaaa agcagcttgg acgtgcaact cggacaattg tcaaaaacga aaaaggacat
    10561 tccctgtttt tgatggttgg tcgctgggga acaaagggcg atgcattgtc tctttatgcc
    10621 atgaatggcg atctcgttgc tcacatcaag cagataagcc tgacattcgg tacgcgtttt
    10681 gagctgtata aagagttcga aaaggtggga accatgcaga aaatattcaa ttggcccggt
    10741 gacttttatt atattcgtag acttcactgg acagtccaag gtgatatcta taatcatcaa
    10801 tatcaaatcc atcatttcaa tcagcaaatc atgaaaatgg ataaagcaac acttttttca
    10861 ggagattact atgtattgga tgtctcaaat gatgaagatg ctccagtttg tatttgtatt
    10921 gctgcggttt tagattactg gctctaccgt aaagacaaaa gtcgcgataa gcctgtttct
    10981 taccgactta acttaagtta aaaagatgta cctgaaatgc ggaaatacgc atttcaggta
    11041 catcttttta gatattcttt tgcatatgat cataaagatg accactgatc atccgctgac
    11101 cgacaacttt gaagtcatgg cgttcgtata attttttagc ctttggattt ttttcatcca
    11161 cacttagacc gatgacggag cgattagctt tcttcgccaa tttaggtaat gcttctaata
    11221 atcgcgaacc gatgccttgt cctctaaaat cttctgatac actgatagag tccaaatacc
    11281 attcgttggg aaacgtttct ttatcaatga acaaacgtgt agacgcatct agctgatatt
    11341 tctctaagat aggtaccagt ggctgatcga tgattgattc ttcttcatct gtataaccgt
    11401 aagcaactcc tgcaacttta ccgtctattt cttctacgat acctcgtgaa taactgtaac
    11461 gataatttgg atcagtgatt gcttcttcta aaatctctaa agtcttttgt tcccctgtct
    11521 gcttcaaaaa tggcagttcc atatctttta gaatcactaa gatcaatggg gcgatttctg
    11581 gagcatcttc ttttctcgca aaacgaatca tagtttcctc tccttttctc cattctaaca
    11641 tgcagaaaaa gaaatcgcaa aatcgttatt ttaaaaaatc ttcaatcttt tcagtaatga
    11701 aataaccatg agtaacgatt cttgccgctt cagttcggca ataagggcag acgactggca
    11761 tttctttatc attatgttcg actacattat tgacttcttc gtatgcaaat ttttttccgc
    11821 aattagggca tttcattttt tccatgttat ctcaacttcc tttcatcttt actctcttag
    11881 cttactctta tattgtaaaa aagagaagca atatccttct tctatgcaga tatccatacc
    11941 tgacggtcct gttctccatc tattaatcgg actttataat aaggacgttc atctttgact
    12001 agaagtgtcc actctttcac ttttgctttt ttcccgtagt agctgactgc ttccttcatc
    12061 gctgcttgcg gttgtttgaa ctcttccgtt ttttctatat caaaaagatg ttctgcggcc
    12121 ttttccgtgt catttcgtac ggttgttttc ccagtggatg gattgactgc tatgacttgt
    12181 ccttcacaaa aaaactggta ttcatatgtt gcattcgtag gattctccaa atctttttca
    12241 actgcaaaat gaatagcaga tatgttatgt tcttttgttt cgttttttac gattgctgct
    12301 gcttttttca atgagatccg atattcctgg tcactaatat catccatgtg aaaagcggca
    12361 ttgagcagtc ccgtcatacc ggtaatccct attacgatga gggcagcaac accaaacatc
    12421 atttttttca ttcttcattc tcctgtttct tttttttaaa gaatatcaag aaatgaacga
    12481 atgaaaaatc gaatgaaacg aaaaaaaagt gacaattttg ttatctttat tcgtgcagtt
    12541 ctaatggcaa tccgtctgga tcaaagaaaa acgtcatttt ttttcctgta aaggtatctt
    12601 ctctaatcgg ttctgattcg atccctaagg actgcaattc tgcaattgtt tcttcaatat
    12661 cgtctacttt gaaagcaaga tgacgcaagc cacaagcttc tggatagttt ggtcgttgtg
    12721 gcgcattagc aattccaaaa atctccaatt cagcacttcc tagtttcaga tctaatttat
    12781 agtcatctcg ttctttgcgg taattttctc tgattacttc aaatcctaat ttattaacat
    12841 aaaaatcacg tgatttctga taatctgaaa caatgatggc tatatgatgg atcgttgtaa
    12901 ggttcatatg agcactccta ttcttcgttt aagctcggat ctatattaat ctccagttcc
    12961 attgtaaatg tgtcagattg aaaaaaatag ctgtttttcc tttttataag aaaaaaggca
    13021 aaaaaaacag aaagtaggtc ctccaagaca ctcttcctgt tcccaatata ataaatgaaa
    13081 gtaagaaatt atattttcaa gaaaacaaac agaaaattat cataacacaa attatttata
    13141 attgaaagac tagttttttt tcaagaaaag catacaatag attgtgttag taaatacaga
    13201 acacactaca tttagtgtct taaggagtcc tcatacatga agtcatattt tcagttttta
    13261 gctcatcagc taaaaggctg gccgcaacaa aattattatc ttttcttttt cagtcttggc
    13321 tgccaggtca tgactcttgt caatagtccc atcacttggc tgtccgttat tacatttatc
    13381 ggtaccacgc ttggtgtatt gtgtatttta tccatcaatg cggcaaaatc agtcaacggt
    13441 attttaggta ttttgtctgc gatttgtttt attatcgttg ggttttcagc aaagaactat
    13501 ttaagtatcg gtgaacagat tgcttatatg atcacattgg atatccccgt attgttaagt
    13561 aaagaatgga atcataatat ggcttcaaaa atccgaaaat tcactggaaa aagctggttt
    13621 atcgcaatca tcagtacttt ccttgtctat cttgtttcag gattcctgat tggtcgttat
    13681 acagatgatc ctcggccgtg ggtagatgcc attgcttttt ctattagctt gactgctggt
    13741 atcatcagtt tcttgcgtta caacaaccaa tattactggt ggttagcttc agggcttgct
    13801 caaatggtcc tttggtatat ttcatttacg catggatctg caagtttggc gatgttcgtg
    13861 aatagctctg tttacttgct gaatgatgtc cttgcattta ccgtttcacc ttggtataac
    13921 caaaaagaac gtgaacgtat gcaaaaaatg gaagaagaat actacaacgc acaacaacac
    13981 gcataaaaaa actgagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
    14041 cgtataaacg gcggaatcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
    14101 tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc
    14161 ctctattttt tatagtaatt ccttcagata ctttcttgcc tcatcattga aacgcagtga
    14221 ttcttctaac ttctgataat attccaacgc ttgtttctga gcttcagtaa aacgataatg
    14281 ttttcctttc catttgtcga ttgtctcttg cacatttcca gattggatat ctttcactac
    14341 tagttgccat ttttcttttg agactgtacg gtctttctta tcagcagatt gtatagcttc
    14401 tcgttcttta cgcaatactt ttaaaaaacc atttctgact atccgaggaa tgctgtcgaa
    14461 gaacccttct gtaaaaccat agcgacaata ttgcttccaa ataaatagat agatttttgg
    14521 ttggatgaat cgttggtttt tcgcaaaata ttccatctga gcagtgactt ctggtgttcg
    14581 ttgacgtttt gtctttctca acacggaaat aaatgtttca aaataagcat taggatgtac
    14641 tggcacttct tcatatacga tggattgcca atctttacgt aaaaatgcgc gatagttgat
    14701 caagaattct tctacatcct ttactaattc ttctgcaaag tcgtcaaata gttctttttt
    14761 tatctgtcgc ttttttgact gcccattgat gtgttcactt attttcttga tttcattttc
    14821 caatgcttga taatccgcta tgtaaatcgg catttctact tggcgaaatt ctgcgagccg
    14881 ctgagctaat tttatatccg gtaaatatgg ctgtttcatc cgacgatttt ggtacaagac
    14941 aaaacagtat ttttgccata gtccttcagg aaaatcgata ttcttctgct taagccataa
    15001 taattcgtct aaggccagat caacttttgt cattccctga tggatcgaag ctgccactgt
    15061 cggagagaca tgcaggtgat cggcaaagtg gttgatgcct agttttagga tctttccggt
    15121 ttttggcgat ttaacgatgt attgcacttt taattctcta ccacactcac aatacaattt
    15181 ttgctggctt cgttttgttc gaaaaaacgg attgatcttg aagtcagaaa acacccaatc
    15241 tgttgcagct aaataatgtt cttccataaa acgagagctg attagatatc ggcggtgctt
    15301 atcgactagt tcccgctgtt ttttcgttaa ttttgcatag atctcccaac gttgactgct
    15361 tgtgtattgt ttagaaattt tgatgggata gcttttgctg atttcttttg gaaaccattc
    15421 ttcaaagacg gattgccatt cttccacggc tgtttctttc ctttctaatt acaaaaagct
    15481 ggctactaac ataacgttag caaccagcca gtgtttcttg ctaaattagt gagttgttcc
    15541 ggcatttccg gaaatatctt ctttattttc tactgctgct tcaactgcat aagtcaaaga
    15601 agaagcaatg tcttctaaag acatagaagg cgtacccact ggacgatcga tcacttgatc
    15661 gggtgagaat ggcacgtgga tgaacccacc tcgaattgcc gggaattgtg tttcgatcaa
    15721 atgaagcaaa cgatacatga tatcattgca gacaaatgtt cctgctgtat aagagatgta
    15781 cgctggtaga ccatgatctt gtacattttt tgtcattgct ttgattggca atgaggtaaa
    15841 gtaagcggct ggaccgtttt cttctaaagc cgtatcgaat ggctgatttc cttcattatc
    15901 agggattctt gcttctgcta gattgattgc gacacgttcg aacgagactg ctgaccgccc
    15961 acctgcttgt ccgacacaga tcacgacatc tggttggtgc tgttggatgg cttctttgac
    16021 tacttgactg cttttttcga atacggtcgg tacttccact ttgatgattt cagcaccgct
    16081 gatttcatct ggcatttttt ttactgcttc ataggctgga ttcactttgt ccccaccaaa
    16141 tggatcaaaa cctgttacta gtactttcat ctgttgttcc tccgtccgat tgatttaaca
    16201 tatggttatc ttttatcata ccattttcct tgaacagcgt aaataaagga aacaatagtt
    16261 taaataacat aacagatcaa acttaatata ttcttataaa aaaatagtgt tccactacaa
    16321 tgttagaaga ggttgcgaca attattctgt cacaacccct ctcttctatt atttaaatag
    16381 aatcatataa gcaatttgga aaactagcat aaataacgca ataaacaact ggtttttgat
    16441 cactccgtat ttgtctttca tctccagcat cgctacaggc acgatattga agttagcagc
    16501 catcggtgtc agcagggtcc cacaaaaacc gcaagtcaat gcaaccattc ctattaacgc
    16561 tggattcgct ccatgactaa atacgaatgg tgcaccgatc ccaaccgtca tcacggtgat
    16621 ggccgcaaat gcattcccca tgattaccgt aaatatcacc atgccaagag cgtaaatgat
    16681 gatgccgatg ttcacatttc cttctggaac gatcgtacct actgcattgg aaatcactgt
    16741 cccaactcca gcgcttgtaa ataccgcacc tagagaagct aaaagcattg gcaacatgct
    16801 taacggccct actgtgccca gcatatctgc tgcatcatct aaaaatgtac ttggtcgatt
    16861 atctcgagaa aagaacatca agattagcat ggctgcaaaa acaccgacgc ccacgccgac
    16921 taaagcacct aaattggtaa atagagcaaa caagatcgca aagacaccga tactcaaagc
    16981 tggaataaaa attttcattc ctagtttgtc tgacattttt tccatatatt cttttgatgg
    17041 aagccggctt tctcctttgc tgactttctt agcaattgct ggtatggtca tcgcaaagat
    17101 caagccaccg ttcacgattg ccggaagcca tcttcctcct gcaataacta cccctaagac
    17161 cgtccagaaa aaggctgacc cataacgatg aggattggtt ttatctgtta aatttcttac
    17221 tccagtgtaa atcaatacga gtcccatcac gatataaagg atctccagta atttatcgcc
    17281 taataaaata tcaggatttg taaaaaagtc catcttattg cccctttcct ggataatatt
    17341 tcttcgctaa tttcttttct ttaagataga agtagacact tgccacaact aaagccgtga
    17401 tagccactgg aacttccact aaagcaagct gtcccaatgt tacgtcatat cctagatctt
    17461 tcagtgttga ttggactagc aacgcccctg ctcctccgat gaatagtact tggccaaaga
    17521 accagcagac attttccata gctgaagcca taccttttag ttcttcttca tgcttaggat
    17581 ggacttctaa cccttttgat tcgatggtcc ccaatgccat cggtaaaatg attggacgaa
    17641 caaatccagc aactccacca aaactcacgt taaaagccgc aaagatcatt cgaaagatcc
    17701 cataaatatc tacaatcata ccagcagata ctttcttgaa tcgcttgatc aaggaagccg
    17761 ctgattcttt cagaccattt ctttcaagcg ttcctgttgc caacataatg atgataaaaa
    17821 tcgccatccc gcgattgttt acaaaacttg tacctaaaag ttctaacatt ccttgaatgc
    17881 caagaccgcc agtcaaagct gtaacaacta aagcaaccat tacgatcaat atcgagtcta
    17941 atttaaatgc aaacccgaga ataacaatca agacgcctaa aaggctaaga taattttcca
    18001 tatattttcc tccataaaat gacaatattt ttttaattat aaagcaaaac acataaaaaa
    18061 tatagttgtt ttctcacgaa atagacacta tttttaaata tttttcgtta tttttttatc
    18121 acaaaaatta tcttacttat tgtaaggtaa agcttttttt aatttattgc ccgataatat
    18181 atagaaataa ggtctagtta ctgttttttt taaatgatta ataaataaca acttctttct
    18241 atgaaattgc aaagcattca tcttcttttc cgctaacgat atatccgtca gtgcttggta
    18301 cactttacta aaaagtatga gattgccgat atctggatag gtgtgctata ctacagaaaa
    18361 agatatacaa tctgccaccg ggtaggataa agaggatgat cattccatta ggtctttgaa
    18421 gggatgattt tcctcttttt gtgtattttt ttatagttaa ggaggaatgt aagtatggca
    18481 tggatggaat taatcatagc aggaattttg gaagtattct ggtcaacgat gatgaaatgg
    18541 agtgatggtt tttctaaaat caattattct ttttacacag tgattggcat gattgctagt
    18601 ttttactttt tgtcaaaggc aatcaaaagt ttgccgatga gtctctctta tcctatctgg
    18661 acagggattg gcgcagttgg ttcggtcata attggtgtgg tcttgtttca tgataagctg
    18721 aatctttcca cttggttctt tgtggggatg ctactcatca gtatcattgg catcaagatt
    18781 actagcggcc actagacact tggaataagt actgatcaga aaaaataagc ctttttattt
    18841 aatattcaat ttttttatga aaaaataaga aataagtcca atgacctagc gacttctcca
    18901 ctcatcaggt gtatgcttgt actatctata gaaaaggagt gaatattttt gaaaagtctt
    18961 tcggtccaag aattatccaa gaactttggt cacaaacaag ccctcagcga tgtcagtttt
    19021 agcttatccc ctggcaaaat cgttggatta gtaggtccta atggcgctgg aaaaacaacg
    19081 atcatgaaag ctattttagg actgatccat tactcagatg gaacaatctg tattggtgaa
    19141 aatgagattt ctccctctag tcataaaggg ttggaacaag taggagcatt gatcgaatac
    19201 ccgggtatct atccattctt aacaggctat gatcatctga agttattctc tgaaaccaac
    19261 gatgtatctg ctatcgacac tatcgtgaat cagctcaaga tggaaaagta cattcacaaa
    19321 aaagcgaaat cctactcgtt gggaatgaaa caaaaattgg gaattgctct tgccttactg
    19381 aaccatccgt ctatcgttat tttagatgag ccgatgaacg gtttggatcc acaagcaaca
    19441 agagatgtgc gaaaaatcat taccgacttg gctactcagg gcacctcttt cttgatctcc
    19501 agtcacttac tcagcgagct tgaaaaaatg gtcgatgagc tgatcctgat caatcaagga
    19561 aagatcatca aacaatgtac aatggctgaa ttgaaccaaa cagcgcaaga tttcatcgtt
    19621 atctctacaa caaatgatct agctgctcga acgatcttga cagaagctgg atatactttg
    19681 gaagatcctg ataaaatcaa gcttttaaaa gaaaacgaag aattattagg aaccgtgatc
    19741 cagctattgt ctgcacaaaa tatccatgtg acagacgtcc agcatctgac gaatgatttg
    19801 gaatcttctc tattggaaat tctggattca gtaaaggggg aagaagaaaa atgacattgc
    19861 ttgtaaaaca agaattattt aaattgatca agaaaaagtc aactgctgtt ttatctgttc
    19921 tattagttgt tttgctcata ggaacagcac ttctagccaa aaaatacacg acgatcattg
    19981 atccagttga aatgactgct cagcttttta gtgccacatc ttggattgtc tttatcatga
    20041 ttgcagctgc aagcacgatc atttcgatgg aagcacaata tggtacattg aaaaatctcc
    20101 tttatcgaaa gtattctcga ggagaaatat tagtaagcaa atggatcaca ttagttattt
    20161 attctgttta tttatacctt ttagctatta ttgtcactgt gttgatgaaa ctgattcttt
    20221 tcccttcgat ttccttcacg gagaaggttt ctactggtca aacactgatc caaagcttgt
    20281 tcacttatac tttgggtagt tatatcggtt tatggctgat tttaagttta gtgttgatga
    20341 ttgcttgctt tatcaatagt tccggcgcat ccatctcagc aggaatcgtc ttttattttg
    20401 cttcgtcaat catatctggt atccttattg cactcatcca aaaatgggag tggatcaaat
    20461 ggaatccgat cagtatgttg aatctgcaaa accaaattgg aaacgaagag atcatgaagc
    20521 aattgactca tctttctact aatcaaatgc tattcggtaa tttagcttac atcgttttgt
    20581 tcttagcatt aggatatctc gtatttaagc gaaaaaatgt atgatgactc atcaaaaaag
    20641 aggctgtgaa agagacgttc agccttattg ccgattggct gtctcttaaa caccgtttat
    20701 tctgaaaaag gagctgtgac aacatttgtg tcacagctcc tttcctagta tgtttttgaa
    20761 taaacgacag gctcacaacc tctctttata cttcttttat acttctatac gtgaagcatc
    20821 ccagtggcaa ataaaaccat tgcaatcact ccgaccatcg tcacacctat aagtaaaatc
    20881 ttatctaagt ggatatgttg tacaggctcg ttttgctcct tacgtgcttt gaggtaaaaa
    20941 taaatgcctg gcaagaaaag aacaagaaca agcattactt cattaaaggc tacgactagc
    21001 atagcccata cttgaaaggc acttgctaaa agccctacta aaaattggac ccattctttc
    21061 cgctgataag aaagtttcat ttggtacaat cctaagaaaa gccacgtgaa gaaaatagct
    21121 gctgtaccaa gtgtgtaagc aaagttataa gcactgtcag tgaataataa tgaaagtaaa
    21181 aagacattcg tacataacgt cgtcaatacg atagcataag ttggtgcccc atttttgttg
    21241 agttttcccc atgctttagg caaaagtccg tcttcagcca tgtttcccat tgtttcaatc
    21301 ggtaagatcg tccatgagag ccaaacacca actaaagaaa tgatcaaacc gccgttgatc
    21361 acgactgctc cccatttacc aaccatcatt tcaaacacat agcccatagc aggctgcgcc
    21421 ttgatcgttt gcagctgatt catcgtcatt gtcccatatg gaagcatgga aaccaagacg
    21481 taaataataa tcaagccgaa tatccctaga atcgttgctt tcccaacatc tgattgtttt
    21541 tttgctcgtg aacctacgac agaggctcct tcaataccaa caaataccca aagcatcacc
    21601 atgatgcaac tcaatacttg actggaaaac gggatcggct gtccattcgc attcaatata
    21661 atgtcgctag aaaagttatc aatgaattgt tggatcgaaa acgtgctgat agctactaca
    21721 ataaataaaa ataatggcac taatttacag aatgtgacga tagcattgac gaaactagca
    21781 ttttcgactc cccgattcac tagccatgca tatccccata aaaagactgt actcatcacg
    21841 atcgatgcca gattttgccc accttcaaat actgggaaga aatatcctaa tgcgctcatc
    21901 aacaatgttg caaaagctac gttgcctaac cagcatgtga gccagtaacc ccaaccgctg
    21961 ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac taaagatccc gccttttaaa
    22021 tctggacgtt ttttattgat attattgatc gacaaggaga gcatcaacat tgctgttcct
    22081 acgagtaccc aactgaaaat agctgggccg ccagccgatc cggagatgtc cgtcataata
    22141 ccaaatacgc cacctccaat cgctgaacca attactaagg cagcaagtgg aattaaacca
    22201 attccttttt tctgttgctc tgtatccatg taagcatcct ccaaaataaa accgctttta
    22261 tcaaaaagta taacaaacct tttttatttt tacttaatta ataaaaactt taatagaaat
    22321 ataattctac cggctttttt ctcattatac aagcaaagcg cctagtccct ttccaaatta
    22381 caccaccaaa aaacttaaaa aagatgctat actaaaagga aagaattgga ggatgatcca
    22441 tgaacatttt atatatatcg atttcaggaa atacgcgtgc ctttgccaaa catctagcag
    22501 aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa
    22561 tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt ttttactttc gttcctactt
    22621 atttagacgg cggaaatggt ttagataacg gtgatacaga aatcttgacc gaaacgatgc
    22681 gtgaatatct ggaacatgaa gacaatcata aactttgtct tggtgtagtc ggtagcggaa
    22741 ataaaaactt caataatcag tattgtctaa cagccaaaca atatgcacaa acatttggct
    22801 ttcctttctt ggcagattat gaattacggg gaacaccatc tgatgtgaca cgaatttatc
    22861 agattttagc agaaaacaat aaataataaa aaagaagaag ctgaaaaata gatttcagtt
    22921 tcttctttcg tttctattgt ttctatcata ttaagctttg acatccatca caggtgcttt
    22981 tgcttttgct ttacctgttt tttccaagac aaagtttttt acacgatcca tattggttac
    23041 caagacgatc atggatgttt ctttgcctgc tgctttgatc gcttctaagt cagcttctgc
    23101 tacaagcgta tcaggtgtca cttgctgccc ttctgttact ttcacatcaa atggcgtacc
    23161 gcctaagtca actgtgttga ttcccatatg aagcaagatt tctaaaccat ttggcgttgt
    23221 gatcccaata gcgtgtttcg ttgggaagac agagctgact accccagtaa ctggagaata
    23281 gatatgtccg ttttctggtt ctacaaagta gccttcaccc atcatctttt gtgcaaaaac
    23341 tggatcactt gctttatcta aagaaacaac tgttccgtca gcaactgatg agaggatagt
    23401 cacactttca gctgaagttt gaggtactac tttttcaggc acttcattta ctgtggctgt
    23461 ttcagtcgct aattcttctt tttctaaacg gtctttagga ataccgaaga agaatgtagc
    23521 aacgaagcca ccagcataag ctgccaataa tccaaggatg tagcctaacc atttaccgtc
    23581 agagatcaat gggatcaaag cggctcctga aggtccaata gcgatagccc cgacatttcc
    23641 gatcattccg atcactgccc cgccgatacc tccaccgata caagcagtaa taaatgggcg
    23701 tcctagaggt aatgttactc cgtaaatcaa tggttcgccg attcctagga tcccgactgg
    23761 taaagctcct ttgatcattt ctgttaattc tttatctttt ttgcagcgaa tccataatgc
    23821 caatgctgca cccacttgtc ctgcaccagc catcgcaaga attggaagca atagcgtacg
    23881 accagtttca tcaatcattt gcacatggat tggagtcaaa atttgatgca gtccgaacat
    23941 aaccatcggc aagaataatg tgcctagaac gaagccggca aatgctccac caacgccaag
    24001 tacccagttg atcccaccga ccatgccatc agaaatgaaa cctgctaaag gcatgatgaa
    24061 gaaaatttca atcagaccga tggcaatcaa tgaaagagtt ggtgtaacga tgatatctac
    24121 cgcatcaggt actactttat gcagtttttt ctcaacgatt gacaatagcc aaacagcgaa
    24181 gatgacacct aggatcccac cttgtccagc tgcaagagct tcaccagtaa ataagttttt
    24241 cagtggtgct tccggattca tccctgtcag catcacaacg gccccgataa ctccaccaag
    24301 agttggtgtt gcaccaaaaa cttgtgcagc attgatacct gtatagataa ctaggtagct
    24361 aaacatcccg tttttcaaga tgttcatcac atcgatatat tgttgccatg ttgctgcatc
    24421 aagtgttccg gctgtgacta agtttgctaa aactgcggca atccctgcca cgatcccagt
    24481 accgacaaat gcaggaatca ttggcacaaa gatattagaa atgtctttta ataccgattt
    24541 gattttagat ggtttttgtt tcgatttttg tgccgctttc atttcttgtg cccgttcgtt
    24601 tacacggtct tttccagatg ctgatgaatt tgctgtagct gtacttacag aagaaagttt
    24661 ttctcctaat ttcacgcctg caaaatcaac catttgctgt gctactttgt tcactttccc
    24721 aggtccgatg atgacttgaa gctgttcatc ttctactaca ccaagcactc ctggaatcgc
    24781 ttttaattca gagacattta caagatcatc attgatgatc ttcatacgca cacgagtcat
    24841 gcagtggaca atgctttcta cattgcccat gccgcctgcc cctttataaa tttgttttgc
    24901 cagttcttcc aaagtcagtt cgttttttgc catggtcttt tccccctcat aaggtccctt
    24961 ttacaaatcc gtttgcttca gctaattttg cttgagcttc ctctttatca ctatttgtca
    25021 aaatcatgac gatagccagc ttaacatttt gatctgcttc ttcaaagtac ttttcagctg
    25081 tttcgtaatc agcttctgtc gcttgcatga tgattcgttt tgagcgttcg attagttttt
    25141 catttgttgg tttgacatcg accatcaagt tattgaatac tttacctata ccaatcatac
    25201 tgatagtaga aatcatattc aagataagtt tttgtgctgt tcctgacttc aatcgagtag
    25261 agcctgtcaa aaattctgga cctgcatcga cttcgattgg gaaatccgcg tgtttactga
    25321 tttcggcttg tttattgcaa gaaatcgttc ctgtgacagc accgatcgat tttgcgtaat
    25381 ctaatccacc gatcacatat ggtgtacgac cgctagctgc gataccaata actatatcat
    25441 tttttgtcaa attcagcttg attagatctt cttgccctag ctcataagaa tcttcggccc
    25501 cttcgacagc tacagtcatt gcttcttctc caccagcaat tagacctatg accatttccg
    25561 gttctacacc gaatgtcggt acgcattcag cagcatctaa cacacctagt cttccacttg
    25621 ttccggctcc catataaatc agacgtccac cttttttaaa ggactcaatc gtgttagcaa
    25681 tcactggttc gatacttgtc agttcttttt caaccgcagc ggccactttc tgatcttctt
    25741 tgttcattag ttgaacggct tgagaaatac tcatctcatc taatccaaaa gtttcttggt
    25801 ttctgcgttc cgttgttagg ttctctaaat tgattttgct catttttatc ttccttcttt
    25861 tcttataaat tgatagttca taccagcttt gatttggttg atcaatggta gctcttcttt
    25921 gataacttta gctactacat tcactttttc atccgctggc aaatcatatt tggttaattg
    25981 gatctcaccc atatagcgca aatacttttc gttatctaaa gtaatagacc ctttcatacg
    26041 cgtagctgta tttcttgctg ggataggcgg aatctctcga aaacgtgcat ctacgcttcg
    26101 tatgacatct cttgcatcgt cttgccgatt cgtatgcttc cctaatatat attcgtaaaa
    26161 ttcttcatct acaggttccg tatggagcaa gactgtctgt tctttttgga aagaagcaaa
    26221 ttgctcctga acgttttctg acagacctgc atctccgatg taaacaatat ccgtgttact
    26281 tgctaataga tctagagcgc ctgcaagagg atggatccct cgatgttctt ccaaagttgg
    26341 caatccttga tatagtgggc ctctcaactc tgtatctcct ggtacaaaac cttggacaag
    26401 gagaccttga attttgagcc attgattttt tttctgatac cattctttgt ccagccctgt
    26461 ttcgggacgt ggataataat tatgccaagc ttcaatctgt gagaaatcag cttctgcttc
    26521 tttcaattca tcgatatctt taggcgtgat cgtgctagca ttcaaactga ttttcaattg
    26581 atgtgaccag tcggcaatct gctgattgga aatatgataa tccattctta aacctgttac
    26641 accaattgct ttcaaaggct taaatcttct aatgaaaaac ccgctcgttc caatgcctct
    26701 cctgaaatat cgaccatcaa ttctagttta ttctttttcg cttcgcttcc tagttcgatc
    26761 aatcgctttt gatatgccga aacatcatct tctggtatat gcatggaagt gaaaataccg
    26821 acaaagccat tggatgccat ttggcggata tactgtttct tctcttctga caaagaatcg
    26881 ttcataaata ttgaaaagcc aaacatatta tccctctttt tctgtatccg tcttcatttc
    26941 tatgactaga gtatatatct aaagaaacaa aatttcaata cttttattaa aataattaaa
    27001 tttcatttct aaaggttgag tttatattat caaaagaatt gtcataacct aaaaaaggca
    27061 ctgctaagca aaagcagtgc cttgaaatat tttatttaaa taacacaaaa acataattac
    27121 tcataagaaa ccaattctgt attatctacc atgaatgtaa agaacccctc attaggcatc
    27181 ggatgaaaat aagcattgat cactcttatc aaggcattat ggcaaactaa cagatagaca
    27241 ttttcttcgt cttcgatgca ttctttcaat aaaggaacga tccgtgcata cacatctaag
    27301 actgactccc catttgggaa tcgtacagcg aacgctaaac gtgctttttg aaagttctca
    27361 tctttgcttg gcagtccatc ataatcccca aaatccattt ctaccaatcg ttcatcaacg
    27421 gtcagcggca agctgagacg atcagcaact gcttgtgctg tatctcttgc tcgttgaaga
    27481 ggggaatgga tgattttcgt gattggaaca ggcaaatcta ctaccttttc tgctagtttc
    27541 tgtgcctgca tctgtccttt ttcagtcagc ggcacatctg cccgtccgca aactaatcct
    27601 tgtgcgttcc aagttgtttc tccgtgtctc gttacataaa gcatgtgatt ctctccttcg
    27661 ttggcaaagt tccccttaac cataccttgt tgttcttgag attacaaggg ttagattctc
    27721 tcaagagaaa taatctcagt agatacttta tccaggaagg tttgatcatg ttccactagt
    27781 aacatggcag gtttcacgtt caagatcagt tgttctaatt gttcttgatt gaagacatcc
    27841 aaataattca atggttcatc ccatgtatat agttcagctg gctgtgataa agatttagcc
    27901 aattccactt ttttccgttg gcccatactc atctgctcga tcttgttatg aaaaacatct
    27961 ctttccatcc caagctttcg gaggttgttc aaaaatgctt ggtagtctac ttggtttttc
    28021 tctgcaaatt ccgccaacgt tcctcgattg tcttcataat tttggcttgc ataactaatg
    28081 ctcagatgtt ttggctggta tttttctcct ataaccttgc cgttgaatgc ccccagaaga
    28141 taatggataa tggatgactt tcctgcaccg ttaggacctg aaatcgccac ccgctgatga
    28201 ggctcgattg taaaatgaat tggctcgaat aacagatttt cataccctaa ttgaagatct
    28261 tctacgctta aaagtcgctt atggtgagac gcttggctat tcatcgtcaa agaatcgata
    28321 tattcgatat cttttaatag tttttctttc tcgctgatct gggtctccat ccgattcacg
    28381 attgctttgg atcgtttcat cgtccgtgca gcatcagcac ccactgctcc tttattcact
    28441 cgtctagatt cagtatcgat gaatccgact tgtttctttg ttttatctcc ttctcgggaa
    28501 cgagaccatt cggctttttc agctgctgtt ttctttagcc tactgacttc ttttttcaat
    28561 ttttcatttt gagccatttc aaattcatca cgaagttttt tctgttcttc atagatagag
    28621 aaattccctt gataaagttc cagttgactt ttttcgattg ctaaaacatg gtccactact
    28681 tcatcgataa atccccggtc atggctgacc acgatgaagc cttgtttttt ctttttcaaa
    28741 taagccgcta cttgttttct accagagata tccaaatgat tcgttggttc atcgattaac
    28801 gggaaatgag tgtcatccac aaataaaagt gccagtagga cttttgtctt ctctcccccc
    28861 gatagtgtgc tgaattctct ccataagatt tcaggatctg tttgcatcaa ttggagctct
    28921 ctttcgattt cccatatctc aaaatccgta atatcattta acacgtaata ggttaaacgt
    28981 tctttatctt ttgtctgttg cgggaaatag gcaaattctt gctgatggat tacttgccct
    29041 tgataaggta gtttgttttg tagaatattc agtaaagttg tctttcctcg accgtttcgt
    29101 ccgataagtc ctagtttcca ttgtgtgtca aaatttagat ttgcttgttc aaataataat
    29161 gtgccttggc tgtcgtagcc gaatgtcaga ttttttattt cgattttaga cattttccat
    29221 cacctcgtaa aaaaagacca gtcctatttt cagaactggc taaagaggat gcaagggttg
    29281 gatacacaca aaaagaccca tacatcgaaa aaaccaagcc aatcccgaaa atctgcacac
    29341 tgcttccttg tctccacaag gtacagtctg aatgcagctt tcatcaatcg acttaatttt
    29401 tcaatgcacg gatccctcac tttgtcctat atttgaaatt acgatagcat aaagaaaatg
    29461 attttgtcaa ctacttcggg aggatggtga aaaatctgct aagtgtctgt cctaagattt
    29521 ttgttttttc taatgtttgc ctgtgttcca ttgcttcttc taaacctatt ggacccaaat
    29581 gaatacttaa gacaattggg agaaaatcag aaagtccttc tagctcttgg tctctacttc
    29641 cgcaaatagc gattattggt tttcgatatt tttttgcaag cactgctaca ccgtatggaa
    29701 cttttccgtg gatcgtttgc tgatcgatcc gcccttctcc tgtaatgatg acatctgctt
    29761 gttcgatctt tttttccaat ccagtgattt ggctgactaa atcgaaacca gaaaccatct
    29821 tcgctcccaa caaaaaaagg gctccaccaa taccgcctgc tgctccactt cctggaatat
    29881 catctaaatc cacctcccag aaatctttta tttgctgagc aatctttcct gcttgtttat
    29941 ctaagaaagc tagcgtcgta tctattcctc ctttttgtct tccaaattgc caagcagctc
    30001 cttgttgacc tgtatatgga ttggtaacat ctgcagcgat aatcaatttt actttagaaa
    30061 caagaacgtt tcttcgatca aaatgaagtt ttttagtaga taataaagga tttccttctg
    30121 tgtacccttc taaagaagca cccaaggatt ggagtaatcc tagaccacca tcgctacacc
    30181 cgcttcctcc taaagtgaca ataatttgtt ctacttcttg aagcagtgca tctttaataa
    30241 gttctcctaa ggcataactg gaagcaagtc ggatagtttg atccgttggt ttatcaagta
    30301 gatctaaccc caatgcttgt gcagattcga tgactgcggt gttttttcct tgccaagttg
    30361 tcaataaata gctgattttt cgctgacgga ataagagatc catcccatca atggtacgcc
    30421 atgttcccct gagcgcgtaa tagattgctt ctgcagtatt ttctccgcca tctgcaatcg
    30481 gcacattgat ttttcttcct ttacgatctt cccagccatt tagaactgcc tgatttagtt
    30541 caaatgaaga agcacttcct ttaaatgaat caattgccgc caaaatatcc atctgtatcc
    30601 ctcactttct tcttaagtaa gacacttttt ttcatttttt tcaaacaaaa caatgctatt
    30661 ttcttccatt tttctatata ctaaaaggga actcattgaa taaaggagag aaaaaacatg
    30721 aaaatcgcaa tagctggggc cggagcaatg ggcagccgaa tcggattgat gctacatcaa
    30781 agcggaaatg aggtcctctt gatcgatcgt tggcctgctc acattgaagc cattcgtaca
    30841 aatgggttga ttgctgactt taacggtaaa gaagtggtcg caaaactgcc aatctattca
    30901 ccagaagaaa tcatcgaaag caacgaacac gtggacttga tcgtagcatt aacaaaagcc
    30961 aatcaattag atgatatgtt ttgttcgatc caatccatta ttactgacaa tacttatgtt
    31021 ttgtgtttac taaatggatt aggtcatgaa gatgtactgg aaaaatatgt gccaaagaaa
    31081 aatatcttat ttgggatcac gatgtggaca gctggtctag ctggtcccgg aaaagtcaca
    31141 ttgttaggtg atggagaaat cgaactagag aacttggaac cagaaggcga agcatttaca
    31201 aaaaaagttg tagaagtctt tcaagaagca aatttaaatc cgatctatag ccataacgtt
    31261 cgttattcca tttggcgaaa agcttgtgta aatggaacat tgaatggttt atgcacgatt
    31321 ttggattgta atattgctga actaggcgcg caaaaagcgg cagagagcat ggttcgaaca
    31381 atcgtatctg aatttgcttc tatcgcagca aaagaaggaa tcatactaga tcaagaagaa
    31441 gtgtaccaac atattgcttc tacttatgat cctgacaaca tcgggttgca ttacccatct
    31501 atgtaccaag acctgatcaa aaaccatcgt ttgactgaaa tcgactacat caatggtgcc
    31561 atttggcgaa aaggacaaaa atatgatatt gctacaccat attgtgcctt tttgacccaa
    31621 ttagtccatg caaaagaagg aatccttggc gcagaataaa aggctctctg atgagaatca
    31681 gctgaatcag cagctggttc tcttttttta agcccaataa aagatcaaaa agaaaacaca
    31741 actctcatga tgccataaga attgtgcttg tttgtttcag tacataatct cttcaaaatg
    31801 taggtttttt atttgttttc aacagaggag aggtcatatt cgtattgctc ataaatagtg
    31861 gaatcgttag tgataaatcg ttttgcccaa ttttctgcat cataaaattc caatagctct
    31921 gataggattg gcttaaatgg tgtatgcaga aattccggta attcatctaa atcacgttct
    31981 cgaagcaaaa aaatattttc aggacgataa aaatgataat gagcaatcgc ttgattatcc
    32041 gtcactagtt tcttttgaaa atacatggat tcaaatgtcc gcaaagtcac tccttgctga
    32101 ccctcccgca acagttccag aatcccgcgc gattcccctg tttttttcag gtactcttca
    32161 taagtaagat atccaggttg ttcattccct cgatgtggaa gaatgaaata gttggtcgta
    32221 acatctcttt ttataaattc ctgcttatag ctttctgctc gttcttttct cccattattc
    32281 gttgcaccaa aaaatagatc cgtctttggt tcgctagaag aaaaattcat atgtttcgaa
    32341 taaaatgaac tattatgttt cagcccaagt tcttttgctt ccaaaaaatc aaaatgataa
    32401 atctcatcga ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg ttctgctttg
    32461 aagtgattcc aaaaaaataa cacaattttt gcattggtaa atgggcgcac gcgtcgagcc
    32521 atctccatat caaatccata atctaaaaaa acgatcagct gataatttgc caactgttgt
    32581 ctagctgatt taatgaattc cgactgtagt aaatcttttc cggaccaaac atctaaagag
    32641 cggatggaag gcaaaaaata aagtgattta ttttttgcaa ctagcagtac gtcatttttg
    32701 ttcatctttt ccgcctcact ttctctgtca ctttttccta tgatagcctg tttaaatagt
    32761 aaaaagccag taaaacgttt cctatatttg tatgaaaatt taacatttgt atgaaaatat
    32821 gatgaaataa atcattcttc tattaaaatt cgcctaaaag ttttctgaat agttgtaatt
    32881 gtctgaaaat ttgttatcat tagtcattgt taaatattgc tatttattct taatataaat
    32941 tttgattgtg aatttaaaag aaagaggtca tttatggaag ataaaaaatt gaaaaaacaa
    33001 cttagctcca gacatatcac catgttagct ttaggtggag caatcggtgc aggtctattt
    33061 aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg tattgattgc ttttttgatt
    33121 ggtgggctga ttttatatat cgtgatgaaa ggtctgggaa aaatcgtttt aagcggtggc
    33181 gacacgcatc acggattatc tggtttgata cgcccttatc taggtgctca ttctgctgat
    33241 tttactgatt gggtctactg gtctatgtgg attatcaata tcatcgctga agctgttgca
    33301 gctgcttcct ttttgcagtt gtggttcccg cacattccag cctgggtatt tgtttttatg
    33361 ttggctgttt taactacgat catcaatctt tattcggtac ggttattcgc agaaacagaa
    33421 tattggctgg cttttgctaa aatcagtgtc attatcttac tgatcatttt tgctacttat
    33481 cttgtcggtc agcaaatgtt gggcacaggc gttttcccta cccttcagca attgactgac
    33541 catggtgggt ttactcctca tggtatgaag ggaatcatca gttctttgct tgtagttatc
    33601 tattcttacg gcggttctga actgattgct atcacggtaa gcgaagcaga tgatcctaaa
    33661 cgtgcgattc ctaaagcaat caaaggtgtc atgggaagaa tcatttcctt ttacatcatt
    33721 cctttgtttt tgcttttgat tctttttcct tggaatactt tagcaggcac gaatgtcagt
    33781 ccttttgtga tggtatttga aaaaatgaat atcccatttg cagcggatat cgtcaatttt
    33841 gtgattgtcc ttgctttgtt ctcatctatc aattcgggtg tgtatgcatc ttcaagaatt
    33901 ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa aactagctgt tttaaacaag
    33961 catcaagtac cgcaacgtgc tgttttattt tgtaccagtg ttctttatct tggtgtagct
    34021 ctttcttatt ttgtcggtga caaattattt ggctatctgg caggttccct ttcgtataca
    34081 gtattattga tctggatcat tatcagtgcg gcatcctttg tactggcact aagaaaagga
    34141 acattatggg ataaaggaat cagctttttt gcattagctg tcttaggatt gattttcctt
    34201 ggtatcctgt tcacgaactc cattggggta actgtattga ctggcctgct ttacctgttc
    34261 atttatttca gttatcaaaa aaagaatgat gcgttcgtat tgacgaatga atgaaatcga
    34321 tagaaaattt tattttattt atgtggaata gatggagcta ttgaagaaag gagttgtgac
    34381 aaaagtcttg ttgcaactcc tttttccgaa taaacagtct catgacctct tggtaaacgg
    34441 tgttcaaaaa ccctctgtgc aaaataattg attgttctcc atgctcatga catgatagaa
    34501 gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag tattggaagt ttttgttgat
    34561 gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac ttgaatgtct atgcgctcga
    34621 tcatatcttc ttcagtcgta cacgagtatt catggcgttg atgatgggtg cagtcatggc
    34681 agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac aaaaagataa atgttatgat
    34741 cttagccgct agtcttcttg tttttgccgg ttccttgttt atggtacgaa gccaaactac
    34801 tgtcaacgac acagcttgga tgaaggccat gattcctcat cattctatcg ctatcctggc
    34861 aagtaaacga gctgatttaa aagatcctga agtaaaaaaa ttagcggaag aaatcattca
    34921 ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt
    34981 atccgtccaa ataaaaaaag aggatgttcc agaagttatc agcttcaaga aataagaaga
    35041 agctacttct ggaacgccgt gaataagggg ttgtgaaaaa gctgccctgc atcaagtaat
    35101 aagaacagcc aaacaaaaat agcttctcct atttttcgtt tggttaggct tattaccgca
    35161 gatgcaagct tttgaacacc gtttattaag aggttgtaaa atcagcgttc tgacctgagt
    35221 attagagaga caaatggaaa aatagttcct catattttgc cattttttga gctaataccg
    35281 agggtcagct gattgaacac cgtttatcga gaggttgtga aaaagtcgct tagtttcgcg
    35341 tattaagaaa aattttgtga aatggctttc cacatttttg tataaatttg gcttaatacc
    35401 gagaaactgg cttttgaata ccgtttattc gtaaaaggga ttgtgacaaa agtcttgtca
    35461 caatcccttt ttatgttttt attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg
    35521 tttcacaaat ttttttgaga ttccccaacc tgtgaaggca ctgaccatcg aaaacacagg
    35581 agaaagcaaa ctgaaaaata cgaatggcaa gtaagtgagt gtaggaatac caaatgtact
    35641 tgctacgaaa cttccggcaa ttccccaagg cactaagtag ttgattactg ttccaccatc
    35701 ttctagcgtt ctacctaata cagttggatc aaatcctgct ttttcataag tttctttaaa
    35761 tgcattacca ggcagaataa cagataggaa ttgttcacca ataaaaatat ttactccgat
    35821 acttgtcatc aatgttgtaa aaatcaaatt tggtgtgttc gtcatctttc ttgatactcg
    35881 ttccatcacg gttgagatca agccaaattc gatcaacaat ccacctaatg acaaagttag
    35941 aataatcaat gacacagtag gcatcatact ttcgattcct ccgcggctaa gcagtgaatc
    36001 gatttgttgg tttccagtat ttgaaacaaa ccctgattcg atgaccccag ccaacttcgt
    36061 taaggaagtt tgtgggtttt gaataaaaat cattcctact gctacagcga tattcaacaa
    36121 aatcgttaaa attgccggca cctttttcca tgcacacagt aacattaaaa ataaaggaat
    36181 cagtgaccag attgaaatag taaagtttgc ttctagaaca tcagtcactt cacgaataga
    36241 tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac
    36301 aaatgccggg acagttgacc acatcatatg cttgatgtgt ttgaacaaat cggcatcaac
    36361 aatagctgct gctagatttg ttgattcgga taatggagac atcttatctc caaaaacggc
    36421 acctgaaaca atagctccaa caaccagcgc tggatttaag cctagtgttg tcccaatacc
    36481 gaaaaatgca atcccaatcg ttgacataac agtaaaggca ctccctacag cgctacctac
    36541 gattgcacac acaacaaata cagaagggac aaaccactgg acactgatca atttgaaccc
    36601 aatgaccatt agagttggaa tgatccctgc ttgtatccat acagcaatca atgaaccgac
    36661 taaaataaaa ataaagattg ggatgattcc tggtttgatg ccattgatga ttccttcgtg
    36721 cagccaatct gtcgggtatc tttttatcac tgcatacaac atgaccattc cgatcgctgc
    36781 taagatcgtg atattaggtg agacctttaa tccgacaacg ccaatcgcaa tgcctaaaac
    36841 aataaccatt aatacaagtg ttgcttctaa aaaacttggt gttccttttt ttcccatgat
    36901 aatctctcct tttttagttg ttcttatgtt ctaaaaaagt taagatatcg gattactccg
    36961 ccgcaattca tccgcgattc ctcctttata aaaataaaaa gacctgtttt aaactaagga
    37021 cgaatgactc cgcggtacca ccttaattaa aaacagatct gtttttcact ttcaaacttt
    37081 tattcaaatg tatggacttt ccatgtaatt cgtcaattag gcttgatcgg ttcacagcga
    37141 ccaccgactt tctgaacaaa acacctaacc gctactgcgt gaaaagttga cttgtacgaa
    37201 gtttaccttc cataagatca tttgtcaatg aaaaagaaaa tattttttca tttttctgaa
    37261 aattattttt ataactttca cacacaagtt tattttttac tagaaagtag cgccctcttg
    37321 tttttttctt gctactattt cttgatgttc ttgcttgatt ctttctaata gctttggctc
    37381 tttcaacaga tcaagagctg tattggcgag tagttctgct gcaatcgcaa tagaagcaag
    37441 gcctttgtca cttttagcag cagctttgaa ttcttctgtg tgtccggcaa tatatgtctc
    37501 agagattgct accgtaggct ggattgttgg tatgacttgg ctgacatttc ctacatcaga
    37561 agaaccgaga cttgttcttt gttccgtttc tatttcttca tctggaacac ctgcttcttt
    37621 tgcatgctgg aagaataagt catcaaaaga aggagtaacg atgacatcat caactgcatt
    37681 ttggaacaat ccaaactcaa aagtcgtgcc cgtacttatc gcagcacctc gtacgatatt
    37741 ttccactttt tcatatactt tatctaatgt ttgccgattt cctgcacgta aatagaaacg
    37801 tccagcagcg taatctggaa tgacgttggc agctactcct ccgtctgtaa tgatcccatg
    37861 gatattgaca tcttttggca attgcaagcg tagagcgttg attccattaa acacttggat
    37921 caatgcttcc aaagcattta ctcctttttc aggtgctgct gctgcatggg aagcgacacc
    37981 gtaaaatttg atgtcgactg gatcattcgc taaagattct gtcgttttcc cataacgaaa
    38041 agctggatgg acgcataacg ctacatcgac atcctcaaaa aaaccttctc gaacaaagct
    38101 gcctttagct gatccgttct cgcccccttc ttcccctggg gtgccatata cacggatttc
    38161 tccaccagtt tcatcaatga attgcttcac tatgctggcg gctaatacgg agtaagtacc
    38221 aaacaaatta tgcccgcatg catgaccaat acctggtaaa gcatcgaatt ctgccaaaaa
    38281 ggcaattgtg ggaccgggtt tattggaaat ataacgtgca tcaaaggctg ttcggtgtcc
    38341 agctacttct ttttctaccc aaaacctttc tttttctaat tgtttaatca acgctgctga
    38401 ggaataaaac tcatagttac ttacttcagg atgttcgtgg atatctagag caagtgcttg
    38461 atattctggt aaacgatatt ggattagatc cttcaattgg tttgttgaga ttgttgttgt
    38521 cataaaaaat tcctcctatg ctagtcattt ttcttatttt ctcccgaatg tttcccatgc
    38581 aggcacgctt gatccttttg atgttttctc gattactttg actgtgtctt ctgtttgata
    38641 agcgtctact attttttgat acgttttatt gttttcatct tcttttcttg caacgatgat
    38701 attcacataa ggacgtgcat ggtcatccac tggttctaag aagatggcat cagaagcagg
    38761 attaaatcca gcatctacag ctacaccgct attgatcact gaaatgtcta catctgacaa
    38821 agcacgtgct gtttgggaag catctaattc agaaatgtct agattttttc tattctcggt
    38881 aatatcagaa acagtgggcg cttgtccttt tgccttatcc actttgatca atccagcagt
    38941 ttgcaataac agaagagctc tgccgccatt tgtaacatca ttagggattg ctacttttgc
    39001 tccgtctttg atatcagcta catccttcaa agtgttagaa taaattccca gcggtgcatt
    39061 gacagtattt ccgatcgata ctaaatcagt tccgtgttct ttattgtaat tgtctaaaaa
    39121 gaattgatgt tggaaagaat tcaaatcaat cgatccatct gctaaagcca catttggttg
    39181 attgtaatct gtaaatttca cgtattccaa attaatgttc tcctttttca aacggtcttt
    39241 cacgttgtcc catacatctg tatcttctcc aataatccct agttttacga tagtttcttt
    39301 tttgtcagtt gtatctgctt ttccttggct acaccctgcc aaccaaataa ttgctacaaa
    39361 tgtaatgact acaaaacgaa taattttttt cattttttct cctccacttt caatgtgtaa
    39421 tttttttaat cagttgatta ctgataaatt gacttaaaaa aactaagatt agaatcaata
    39481 atgtagccac aatcgttaca tctgtttgga atcgattata ccctctcgat atagcaagat
    39541 tccctaatcc gcctgcacct actgctccag ccatggtagt cagtccgatc acgttgatga
    39601 tagtcaacgc cgagacccga atgataccag gcaaaccttc taacaaatac acacgtaaaa
    39661 tgataccgat tggactggtt cccatcgctt ctgccgcttc gatcacccca ggatcaactt
    39721 ctagcagtgc gttctcgatt tgacgagcaa aaaacggaac aactccaaaa atcaatggca
    39781 ctaaagctgc tttcgcacca atcgtcgttc caactaagaa acgagtagtc aatgctaaaa
    39841 gagaaagcaa gataatgaaa gggatggaac gaatgatatt gatcagctta tcaagtattt
    39901 caaatagcag acggttagct agtaatccat tagggcgtgt cactactaac gtcacaccta
    39961 agatgattcc aatcatcccg ccaatgaatg ctgtccaaaa agacatatat agtgtttgct
    40021 tcgtcgcttc gacaaattca tccggaatca aataaacatt gggcaaatat gttttaaaga
    40081 aatcagtcat tttatcttcc tccttcgatc actttgatac gactatctaa atcgtttttt
    40141 tgtaaaatat ttatttttac accagatttg atcaaataat ccaatgcttt tttccgctgt
    40201 ttggtttctc cagctaaagt aacgatcaaa ctccctaacg gcgtttcttg taataactct
    40261 acattgccat ataaaatatt cgctgttact tgatatttgc tgtacagatg agcgattaga
    40321 ggttcgttcg tctgatttcc aatataactt aattccacca gccatttatt ggcgatctga
    40381 tcggcaaaag cagaatgggc aatgatcgtt tctaatgctt ggtcaagatg agtagctgtt
    40441 cgaataaaat ctttggtcaa ttcttcttca ggattgctga agatctggat gctgctacct
    40501 ttttcaacga ttcttccatc ttccataacc gctaccttgt tgcagatttc tttgacaact
    40561 tgcatctcat gagtgatcag gacgattgtt aaacctaact ggcgattcaa tttttttaac
    40621 aatgcaagga tctgaagggt cgttttcgga tcaagtgcac tggttgcttc atcacataat
    40681 aataccttag gttcattcgc caaagctcgt gcgattgcta ctcgttgttt ttgtcccccc
    40741 gacagttgtt taggataagc agatgctttt tcttccaaac cgacaagttc cagtagttca
    40801 ttcacttttt gacgacgttc ctgtttcgat tttccagaat actttaaaga aaaatcaacg
    40861 ttgtcaaaaa tagttcggct ttccattaga ttgaaatgct gaaagatcat accgatggcc
    40921 tttctttctt ttcgcaattc tttgggagag agctgactca gattcgtctg attgacaatg
    40981 acttctcctg ctgttggttt ctgcagcaaa ttcatcacac gaactaacgt acttttccct
    41041 gcacctgagt aaccgacgat tccataaaca tcgccttttt caatcgttaa atctacagaa
    41101 tctactgcct gtatctcttt ttctttttgt ttgaaacata cagaaacatc tcgcaattca
    41161 atcattaaaa ttcctcctta tctaaaaaaa gcgtcctcaa gaaaatgaaa atgtcatttt
    41221 cttgaggacg cttttttgcg tgttaccacc tcatgttcaa gctgtgttca cacacaactc
    41281 ctcttcaagt acgatcatac tctagcaaga tatcggtcgc agccgtcatc gccttacatc
    41341 tatgctcggg atgaattgct cgatggccat tttccaatag tcgttccttg cttcttctca
    41401 gctaccgaag ctctctgtca aaggttcttc tatcgtactt accatttcaa tgcaaataat
    41461 attcaaaaaa aaagtccttt tactaacata aactatgtca gtaaaaggac gacgtatcgc
    41521 ggtaccacct tttttcggaa gtttccttcc ctcaagccca acatctcaaa aagagagatt
    41581 ggcgttcgtt ttaacggtcg aacagttccg aatttgccta cttatcgaca aattattctc
    41641 caaggccatt ttcagctttt cttacttgac tcttctcacc agccagagcc tctctgcaaa
    41701 gtttccaaac ttactttcct tttcaacgaa atcgatattt tgttgttggg attaatctac
    41761 atgaggaaaa ctgaaaagtc aagtacgaaa ttcaaattat tttttctata tttatttttc
    41821 tgttttttta tttaacattt caataacagt tcgaagatct tttccttccg caagaagttt
    41881 caatttttcc ttttcttcga cggtataatt tcggacaaaa ctggaatcag gtatcaaaac
    41941 aacgcctaat cctgctcgtg aagcagctgt tgctcctact aaagaatctt ccactgcaaa
    42001 ggctgaacta gcttctaatc ctgttcgttc caaagctgtc agataaggaa tcggtgaagg
    42061 cttgttctcc gacacttcgt ctccgaatgt aaacgtgtcc aagtaatcag ctaatttaaa
    42121 gtgatccaag atatctgttg ctcttttcgt aacagtggtc gtagcaatgc ctagtttcat
    42181 atgacgttct tttaagtctg ccaatatttc ttttgcatat ggttttaagt ggatggctcc
    42241 gcttgtcagc tgttgataaa aatattcttc acgcttcgct cggacttcct gtactttttc
    42301 agggctacct gctgctttca ctaaaatatc aaaactttgt ttccagtttt ttccgcccca
    42361 agcgtcaacg atttcttttg ggatatggat atcaaattgc tgaaatcccc aaagccagcc
    42421 atcacgatac gttttttctg tgtcaactaa caaaccatct aaatcaaata ttgcaccttt
    42481 catctataca ttaatccttt ccaaaacaat acacatagtt tagcacgact ggtccaaaaa
    42541 gaacagaagc aaagaaccag ccttctcgtt tataagagtt cttggaatcg acggatatag
    42601 attttcttca cagctgtcac aagcgccaga taagcgatga ctgtagcaaa taaccataac
    42661 cagtaattgc caggaagtgg catcaagcct aattcagcac caaaacttgt aaaaggaagg
    42721 acagaaccga caataattcc taatgtagtg ataacaaaca tggcaaatgt tgcgttgctt
    42781 tggataaatg gtactttcgg tgtacgcagt gcatggagca ctaaggtttg tgtccatagt
    42841 gattcgacaa accatcctgc atggaataat gcgatgaaaa tcactcgctg tgaactatcc
    42901 aatgtatgga aacttccgcc cactacagca ggacaaataa caaaatacat caacaagtaa
    42961 gtagtaatat caaatatcga acttgttgga ccaaagtaaa ccatgaattc cccaatacta
    43021 gatgcattcc atttttttgg ctcgtccaaa tattctttat ccatccgatc ccatggcaag
    43081 gaaatgcagg aaacatcata gatcagattc aaaaacaaaa gttgtaaagg cagcattggt
    43141 aaaaatggca ggaacgtact tgcaatcagc acggaaaaca tattgccaaa attagaactt
    43201 gctgtagcct tgatatattt catgatattc ccaaaagttt ctcgtccaga aagaaggcct
    43261 ctttccaaaa tcgtcaaatc tttttctaaa aggatcacgt ctgcagattc tttcgcaata
    43321 tccacagctg tatctacaga aataccgata tcagccactt tcattgcagg agcgtcattg
    43381 ataccatctc ctaaaaatcc gacagtatga ccatttttac gtaaagctgt cgtcaaacgt
    43441 gctttttgct gaggattcaa ttttacgaat acttcatggt cttcagctac ttgtgctaat
    43501 tcatgatcac tcatttccga gattttctca ccggtaacca attcattgat ctctagacct
    43561 acttgacggc acactgaacg tgtaactaat tcgttgtccc cagtcagaac ttttacagct
    43621 acaccatgtt ctttcagagc tttcaacgct tgtttcgttg tttcttttgg tggatcaagg
    43681 aaagcaagat aaccaatcaa aaccatgtct gactcatctt tgactgaaaa ttctccaacg
    43741 acgcttggat tggtcttttg tgcgacacca atcactcgta agccatcttc attaagtgac
    43801 tgtacttttg caagtacggc ttcttttcgt tcttttgtta atggtgtaac agtaccgcca
    43861 agatcaataa atttagaaat gttcagcatt tcttcaatcg cgccttttgt gatcatttgt
    43921 gttttaccag ctggatcagc aaccacaaca ctcatacgac gacgttcaaa atcaaaagga
    43981 atttcatcca ctttttgata attgatctta tctgtatcta aagtttctgt tgcttcatca
    44041 atgatagcca catctaacaa atttttcagc cctgtttgat agtagctatt tagatacgca
    44101 tggcgcaaaa cacgatcatc ttcatttcct tcacaatcca tatgatattc aagaatgatt
    44161 ttatcttgtg tcaatgtccc cgttttatcc gtacataaca catcgattgc accaaagttc
    44221 tggatggcat tcaaattttt gatcactgtt ccttttttgg ccatcacact tgctcctttg
    44281 accaaattcg tcgtaacgat cattggaagc atttcaggtg ttagaccaac tgctacagat
    44341 agtccaaaaa gaaaagcttc tagccaatcc ccttttgtaa gcccattgat gacgataact
    44401 gctggagcca tcaatgacat aaatttgatc agtagcatgg atgttttatg gatgcctaat
    44461 tcaaagcttg attgaattgg tttttcagat agcgtttctg ctacatgacc aaatagtgtt
    44521 tcatttccaa cagaaacaat gattccttcc gcacttccac tgatgacatt acttcccata
    44581 aagacaagat tttcgtaact tgtttcgctt tgctgatcgt cttcacatac tttcgcatgc
    44641 ttttctactg gatagctttc ccctgtcaat gctgcttggg aaatgaataa atcctttgcg
    44701 gacaataaac gcatatccgc tggtatcata tcccctgctg aaagccgaac aagatctcca
    44761 cagacgattt cttccatagg aatctctgtg tactttccat tacgttttac agctgccgtt
    44821 actttcacaa gtgatttcag tttttcagcc gcttgattgg aacgtacgga ctgaatcaac
    44881 gtcattgtcc cgctgacgat aaccatagtg aagataatgg ctacaccgaa aagatcttta
    44941 tctccaggag ctgctaatac atattccgtg atgaatgaaa taattcctaa tgcgatcaac
    45001 actaatgtaa atggcgtgat gtatgctttc aatatttcta caatgaatgg tgtttttttg
    45061 ccatatgaaa tcgcattttc accgtaattt tctcttgaac gttcgatttg ttcttctgtc
    45121 aatccatttg ctgatgcatg gaatagttca aataattctt gtttcgtttt tccagcaaaa
    45181 gctgcataga ttttttgtgt atctttcttt ttttccatct caatctctcc tttgtgtcca
    45241 aaagacagct gttctcgctg cttttggtct ttagctattc atcggatcga tagagaaaaa
    45301 actgaaaggg gctgagaaca gatagataat agccctggcc tttcctctag atccttgttt
    45361 ttcgaatgac tgtcatccat ttgttctcat ctcctttact aagtaatttt aattcatgac
    45421 ttccaatcat ttttcgaata gttatttttc caattagacc acctcctttt tatttttcac
    45481 cgatggggat ttgcggcaac aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa
    45541 aagcatatta aaaaaagctt ctcagatctc atcgttgagt tttgacactg tacaacgtaa
    45601 aaagcagata cctgctttta gctaccttat gaaaagcctt atctcgacaa ttcctgttct
    45661 acccattggc gtctctcgac gtttttgggc agtagcctat gtttgtatag gagcctcacc
    45721 taacaaatct tattcggttt tccttcgtta atataacaac taaaaaagag gatgtcaaat
    45781 caattttcca tgtttttcat acttttttta aaatacacac gttatattcc tgtctcacgc
    45841 ggttttattt gtctcatttt ccacctcccg ctataataga aatatagtag gaaacgaggt
    45901 gctgttatgg aaagaacact tgttattatc aagccagacg gcgtgagacg ccatttagtc
    45961 ggaagcatca tccaacgttt tgaagcaaag ggactggcca ttgctgaaat gaagtttgaa
    46021 actatgactc ctgaattagc aaaagaacat tatcagcact tgactgagcg cagttttttt
    46081 gatgaactga tcgattacat gacttccggt cctgtcgttt atcttgtgct ggtcggcgag
    46141 gaagtgatcg atatcgtccg taagatggtt ggagcaacaa aagctgccga tgctgttcca
    46201 ggtacgatcc gaggagacta cgcgctgccg gggactgaaa acatcattca cgcttctgat
    46261 tcccgtgatg ctgcggtaaa agagatcgcc cgctttttcc cgccattcga tataaacaaa
    46321 ccgtctgaaa aagcggcgaa gtgatacaaa ttctaaactg ctggtctagt tgctgggttc
    46381 agcaatggcc agcagttttt tctctaagga cctccgttga ttctttccta acatatattc
    46441 gagcactgtg aagaattctt ggtagtcttt cgtcttttct tcccctaaag cagcagaaat
    46501 caatctttga taataatctg ccatcaatgt ttcatagatc actgtacttc tttctttatg
    46561 tgctaagctc cgtttcatat cttcattcac actacgtgcc tttttcaacc gaccattttc
    46621 tatataaaac gaagtcaggt tcattaaaaa agtaaagaga tactgctcat gattgttcaa
    46681 ctgtgctaat cgtcgcattc tttttttgat tttcaaataa ttgttctcaa tgaaccgatc
    46741 actgaatatc caaggaaaat cagctagaat agatagttca aattgaccat agtctggtat
    46801 ctgcatcaag tattcttcaa tgattcggca ttcataacgt aaaaattcaa attctgtctc
    46861 agatacttct gttttttcga ccatgtgaag attcaaaacc aaataataca gatcttctgt
    46921 tttcttaaaa tgcctacggt tattgctgat ctctttataa ggatcaaagc tgtcttcttg
    46981 tacccgatct agcattcgtc tttttttctt taactttatt ggttcatcca gctggtttcg
    47041 catccattca tattctgcaa gtgtcatgtt cattcgctct aaaaatgatc tcaatgttac
    47101 aaatgacatt tttttcactg aatttgtctc aatcccgact agagaagtct taaaacagat
    47161 tccttccgct aattgggact gtgtcaactt cttgttgact cgaagtttct tgatcaattc
    47221 accatcatac ataatagttc tcctttttaa tttaaatttt tccacattca ataaatagat
    47281 acgttaaaaa ggaaaaatac acgaaaaaaa taaaaaaatg tttttttcat aaaaaatacc
    47341 aaccttcaag cattagaatg ttgtctaact actgaaggtt ggtttgattg tttattgatt
    47401 atttttttgc tgttcaacgt ctgttcttgt tactggatat aagttggttg ttgttttatt
    47461 accatttttt gaaaagacac ttgtcgaatg ttcgcctttt tgcttttcga ttgcttcatt
    47521 acgttccgct tcatttttat agtcatattt tttcggatca atcgtctcaa atccgtttgg
    47581 cacataaaac cgcaagagat tttcctgatt cagcttatct gaaagactta acgcagtctt
    47641 gactttttcc tgatctttgg agatttcctc atcgattcct gctgctttcg tatcgatgat
    47701 ttctcctgtc tgattattgt aagcttttcc tcctaggacg gtgtatttgg gtgtcacaaa
    47761 gttcccattt cgaaaagcaa ctacttgatc atgctgagaa gataataagt ctgtaccaaa
    47821 atgaagataa tctttatcat cgattcccaa tagatgtagt aatgtcggca aaacatcgat
    47881 ttctccaccg tattcatgat tgactgtccc tttagtataa ccaggcatat ggatcatcaa
    47941 tggcactcgt tgcatctgtg cgttgtcaaa ttcatcccat gtatctggat ctttaccaag
    48001 agcgcttgct aaatctttat tatctgtatt cgagatccca aaatgatccc cgtaaatcac
    48061 aaagattgaa ttttggtaaa taccagattt tttcaaatac gtaaaaaatt gttccaatga
    48121 ttgatccaga taatgtgccg ttcgaacata attatctact gtgctattgc cagtattcaa
    48181 agatggaaag tcgaaattct tgtcatccgt ataataaggc gtgtggttgg ttacggaaag
    48241 aaatttcgtg taaaacggct gttgtagatg ctcaagatat tgggcagatt ctctgaataa
    48301 atctttatct aatatcccat aacctaacgt ttcgtcggat tcatcaaaat aagatcgatc
    48361 aaagaaattg tcataaccta agtttttata tacatgatct ctattccaaa aactagctac
    48421 attcccatgg aaaacagcgc ttgtatagcc ttgcttttgt ccaaggatcg ctggtgcggc
    48481 ttgaaagaca ttgtctgacc caagttctgt gaataatgag ccttgaggta aaccaaatgt
    48541 gccagtttcc aacatatttt cagcatcact tgtctttccc tgtcctactt catgaaagaa
    48601 attgtcaaag ctgatcgttg cttgattctg gaaaatacta tttaaaaacg gtgtgacttc
    48661 ttgtccgtct accttcatat taatcaaaaa ctgctggaaa ctttctaaat ggagaacgat
    48721 cacatttttc cctttagcga tcccaaatgt ttcgggattt ggctctgcat aatgtttctt
    48781 tgtataatct aaaacttcat caatcccatt actgcttgca tgcgcgcgaa cactgctagt
    48841 cattcctgtt ttgattccgt cataaatagt atacgcatcg attcctagat atttgacaat
    48901 atatgaacgg tcaaatgttc gctgcagcaa ttgtggacgg ttggcttcgc tcagcgctag
    48961 atttcctgaa aaaataagcc ctgaaagaca ggtaacagcg atagccatcg gtttgccgac
    49021 agggttgctt ttgataggta ttttcttgac cttcaaccaa accagtagcc caatgaacac
    49081 agcgatatcc agccagtaga tgacatcatg gggcttcatc aaagaaaagg agcttcctga
    49141 caatccttgc gacactttcg aaaagcctaa tacagatttg actgtaatga aatctgtgaa
    49201 ttcccgataa aaaataatat tcaagtacaa caaaagcgta tttaaaatat caatgatcaa
    49261 aagaatagct aacgctggct ttggcttttt aatatacaaa gaaaggccaa aaaacaataa
    49321 ggtagtcgca attgggttga tccacaagat aaagtactgg atcgtacctt ccacacctaa
    49381 tgaaaaatct gcatagtagg caatgatcgt cttcagccag aaaaagaaaa cagctgtcaa
    49441 aacgattgct aaacgatttt ggcacaattt tttaagttgt gtcacgtata tcacttctct
    49501 ctattcacat tttttcttat tttgctcctg aaaaatgaaa atttcctaaa aaagttgtta
    49561 tcttaagatt tttttattct ttctaaagat tattccgtcc acttttcaca gcataaaacc
    49621 gctgcgatgt aagcgattaa actaatgaag agataaaagt cttgtggtga ccgcttcact
    49681 ccacggtatt caaaagccag tttctcggta ttcgtacaaa aaaagaggct gtgacaaaat
    49741 tcttgtcgca gctccttatt tcttgatgca ggacagcttt ttcacaactt cttccttaaa
    49801 cggtgttctc ctgccaactc ctcggccaca agtcacaata tttataaaac atgagaagct
    49861 gttttttgaa attgttcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata
    49921 cacggtgttc aatcagctga ccctcggtat tagattatgt caggacgcta attttacaac
    49981 ctcttggtaa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    50041 atataaggag ctattttttg aaattgctcc ttgtggctca gagttaaacg gcaggctcaa
    50101 gacctcttga tacacggtgt tcaatcagct gaccttcggt attagctcaa aaaacggcaa
    50161 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaaac gctgatttca
    50221 caacctctgt ttttcattgt ccataggatt gatattgtcc ttgtgatgac tcattcgtaa
    50281 taattgaggc ttgattttct tcacttcccg ttatcgtttg tccactgcca gaatctgaag
    50341 gacttgttcc tgtcgactcg tttttttgag aagagacagt tgttttcttg acttgttcat
    50401 ttgtcttttt ccattccgct aaacgatcgg cgaccacttt tttgtctact ttttcagaat
    50461 aagcggtgat cacttttcca tttttcaaac gggctacttt gtcgccatca tgagtcaaaa
    50521 gcaaaacggt caaatgcttg tctttaaatt gatattgtct gagagaaatc gcactaagta
    50581 cgtcatcgat attaataaat tgattttctt cttttccttt tggcaaataa atgtccaatt
    50641 cgcctttttc gtaattggca gttattttat actccgccca ccaataagtt gagaattctt
    50701 ttttgaaatt atctactgat tctttgcttg ctacgatttt ttctgtttct ttctgagcaa
    50761 gatctgctgt catttttcct gaaaatgctg cggataaaat aagcagtaag aaaaaaccga
    50821 ataaccaaaa gagaagaaac ggtctttttt tcctcatgct ttcctccact cctatttaat
    50881 tttgactatt ctattatata caaaaaatga aatagaaaga caaaaaagtt gaaaaagatc
    50941 gtaaataatt gaacttcttt ttcacaaaat ggtaagaatg gagaatttca cgcatttgct
    51001 ctcaactatt ctttcttaag cctcaaatcc gtttttatct gatacgtctt tgaaccaatg
    51061 acccgatttt ttgattgttc tttttccatc ttgtgctaag tctactgaaa taaagccata
    51121 tcgattctta tatgcattgg tccacgacca gttatccata catgtccaca tatgatagcc
    51181 taaacaattg gcaccttctt gaatggcttg atggacatat ttcagatgag attggacaaa
    51241 ttcgatccga taatcatcgt ggatcatgcc atcttctttg ataaatcgtg cttcgttttc
    51301 tacacccata ccgttttcag aaataaagca gtcgatattt ccataatgat ctcgtacatt
    51361 gatcaaaata tcatagattc cttgttcata gatctcccat ccgcgataag gattcatttt
    51421 acgaccaggc atttcataat tgtcgaaata atcctcaggc attggaccgt ttttagtatc
    51481 gattggtgtt tcttttgctt ttacccggcg tggctggtaa taattgatac ctaaaagatc
    51541 tactgtattt tctttgataa ccgtcaaatc ctctgcatgg atcacaggca tgagatcaag
    51601 ttcttttact atctcaatca attcacttgg aaactcgcct tttacagctg gatcaaggaa
    51661 tgaacgattg aagaacgcat ctgctatttg cgctgcttta acatcttcag ggtttgattc
    51721 gtctcttgga tagcttggtg tcagattcaa gatgatacca ataacaccac cttgctccat
    51781 ttcatggtaa gatttgatgg ctaacgctga agccaacgct tcatgatagc caacttggat
    51841 cgcttttttc atatctgact cttctggata atgccatcga tataaataac ctgcttcgac
    51901 tggaacgatt ggttcattat gagtaaacca tttttttact ttcgaaccaa acaattcaaa
    51961 acaacttttg gcatattttt cataggcttc aaccgtttct cggttcaacc atccgccttt
    52021 ttcttgcaga tacatcggca tatcgaaatg atacagattc acaaagggtt cgataccgtt
    52081 ttcaatcaac tcatcaaaat aacgatgata gaaacgaacg gcttcttcgt tgattgttcc
    52141 agattcatca ggtatcaaac ggctccattg gatagatgtt cgaaatgaat tatgcccagt
    52201 ctgtttcatc aactgtacat cctcttgata cttcgtataa acttgcgagg ttttttcagg
    52261 tcctacttga tcaaagaact tttgcggttc ttttttgtac caataatcaa aaatgttctc
    52321 tccttttcca tcgcctgaaa tccttccttc tgtttgtggg ccacttgctg cagaacccca
    52381 ccaaaaattt gctggaaatt gatatttttt catcaaagca cctctttttc ttttttagtt
    52441 cgttttagtt gattccatgt atgatatttt ccatcacaat cactttttca ttttcttcct
    52501 cttctttttt gattcgtttt aacagctgtt ctgctgctcg ttccccgatt ttatctgttg
    52561 gtggcgtgat cgtgattttc ggaaggatac tcagatcgtt tttatctaaa taatcatagt
    52621 gactgaacgc aatatccttc ccgattctga caccttcttg attgaaacat tcgatggcac
    52681 caatcgacat attgtaattg ctgacaaaaa tagcagttgc ccctttttct aataatgctt
    52741 ttgccccgta atagccgctt tttctagaat agtctcctgt aaagacctct aattggatcg
    52801 atggatcatt ttggaccgct tctttggctc ctgctaaacg ttctcttgct acgtagtctg
    52861 tttgagtggc agcaataaat ccgatttttt tatgtccttg ttcaccatga tgagcgatca
    52921 cttttgctac actttctcga tcattcacta atattgtatc aatacctgct ggtccttctg
    52981 gacaattcaa tgcgataact ggaatactca tttttgtgag catttcaaat ccagaccact
    53041 cttcttcgga taagaagaca atcagcccat cgatcacatg cttcatcagg tattcaattt
    53101 tttgttcaat tttttctggc tgatcggcaa acccgcttaa taataagcta tacccatttt
    53161 cttcagctac tctttcgatc cctgagacaa cttccgaccc aaaacggctg gaaatcccgt
    53221 ttatcaaaag tccaatagag aagcttcgat tattcttcaa ccctttagca atgatgtttt
    53281 ctttatagtc caacgctgaa atcgcttctg ctattttttt catgttcttt tccttcagtt
    53341 gctgaccatt caagtaacgg gaaactgttc ccactgaaac accagccatc ttagccacat
    53401 cacggatcga tgccatacat tctcactcct gccagttaca tttgatttct ctttcttgtt
    53461 gttcgattat gatgaccgca ttctttgcat agtgttgctt gtcatcaata tccatataac
    53521 taaagtcttc agtagagcaa cttcctctca ctattaatta tacttaaaca agtatttgaa
    53581 ccggttcatt tttaatatag cattttttta aaagattgca acactttttt aaaacgcttt
    53641 ctgaaagtgg cggatttttt gcctttttaa aattttattc taaaatttac tctatttgtg
    53701 ggtgttttgt gcgtttttct cttccatccg tttgaagttt tacattccaa tggtataatt
    53761 tctttatcct atcaaatggg ggaagtattt tgaagaaatt aatcgtacta tttattttgt
    53821 ctgcgggact tttgacattg actggctgtg atttgtttgg cggctctgac caatcctcgg
    53881 acgaaacaac cgattcttct gtcttaaaag cggaaaaagc agataccacc aacagtgtat
    53941 atacgctaaa agtacgtaaa gatatggatt tatccgcggg acagttgaca tttacagata
    54001 aaaaaatgga atggacacgc acgtatcaga cacaaggaac tgattcttct gaatcatctg
    54061 aagaaaaaac agtgctgaca gatatcaaga tcactaccaa agatgacgaa tatattattt
    54121 caggaaaaga aaataaaaaa gaaacaacag tcatatttac aaaaatcggt aattaccgtt
    54181 tgaaggatga ggatggcaac atctacagtc tatagtgccg tctgttggat cttcttttgt
    54241 ttctcccctt cttttctgct atagtaaaga agcaagatga ttgtaaagga gacgaaatgg
    54301 atgatcgata gtcctgatta ttgtacatgt aataacaaca aaaatatcca tacagaattt
    54361 aatgaccaag gaagcttgag catatgcgac aactgtcaca aactagtcaa agattcattt
    54421 aaaacatttg aatcttatcc tgttcaaaat tatgtaaaaa gtagaagaag ataaacaaat
    54481 ccaagcaaaa ctcactatgg ctgattttta tgaacttcaa actcagcttc ttcctttgat
    54541 caagcttcat cagtaaaaca aacagtggat catttcagtt tacaaatcaa agagtaaaga
    54601 tttatttatt tcaaatattt gaatataaaa taaccaaacg atagaaaggc ttattctaca
    54661 tcacgtagta atttttcttt cccgtcgttt ggttttttat ttttagccta attcttgacc
    54721 ttttgcatgc tggttatccg taaaatattt tccagcaggc tttccttcca attcagctaa
    54781 tttttcttgg atctgacgga tcaccttttt tgcatcttct ggattttcta caaaatcgaa
    54841 tttatcccca tccacttgta ttttaggaca tacatcaaaa tcttcatacc attgattata
    54901 gcgggtgttc aacgtttcat aataggtata taacgtctca tcgctttcta gctgttcata
    54961 gtcacgccca cgttttttga tacgttctag catcgtgtcc aaagaaactt tgatatggac
    55021 catcaaatca ggccgttttt ttggagcaac atcatctaac tcgtttagca tcgtatctaa
    55081 taagctatcg tactgctgta cttcgatatc cgttacccga cctaaatcag cgtttagatg
    55141 gaacaacagg ctatcttcat agatggatcg gtctagcaca ttgtcatcgt tcactaaagc
    55201 atttttcatt gctaaaaatc gcttgttcaa aaagaagatt tgcaataaaa aagcgtactg
    55261 ctcaggattt gaataaaaga gcggcaatac ttcgttatct tcgacatttt cataaaatgg
    55321 tttagaatca atctcttttg caagcatatc cgtcaaactg ctcttacctg caccaatcgt
    55381 tcctgaaatc aaaatcactg acatacaacc cttcctcttc tataagtact cgcctacgta
    55441 agtttcaaca ggcaatgttt caaaatcata ttcacgtaca gcttggtctg attttaaata
    55501 cgtatggaga ctttgtgtaa tcatcaagtt cgtaggtacc gtctcttctt gtacaatgat
    55561 catcggtttt ttcaacatag ttgccacacc taattcgtaa gccgttcctg gatcgattgt
    55621 ttgatgctca aagtcaagga tagcaacaac aaattctgca tttgtcagtt cttccatatc
    55681 tttttcgtat acttcctgcg cccatccagc gctgaatagt tcataattgc tttcttggtg
    55741 atgacgtgga gagtagaaac ttgtgactgt tttgttttcc tccaacgctt tttcgatacg
    55801 acttactcga tcgatttgct cctctgaaaa aaatggtcct gctaaatata tttgactcat
    55861 tattttgcct ccttcaataa taactgattt ttaatagata ctgctcgtaa ttcattgacc
    55921 agttccaatg gattgttgga aaagattact tgatccaacc attttttatc cataaaatcc
    55981 tcttttgcaa aagtttctaa taattgttgc aagccgctgt aataaccgcc aatattcaat
    56041 attccgattg gcttgtcggg cgtcagaccg attgctatcc aagaaagaag ttgtgagaat
    56101 tcctccaatg tacctagacc tccagggaaa acgaggacag catctccctt ttcaaataat
    56161 aatacctttc tttcatcgat cgtttctgta ggaataaatg tagtaacatc ttttccaggc
    56221 aattcagttt caaaaagccc attcggatag atacctgtga cttttccgtt attttccagt
    56281 actgttttac taatcactcc cataatcccg gagtatgaac caccgtaaac cagttcgatt
    56341 tcttcttgtg ccatgtattt tcctaacgtt tgcgcaataa atttgtaact ttctttatta
    56401 ccaaaacggg aaccgcaaaa aacagtaagt ttcataaaaa aacactccct aactcaaaag
    56461 tctatcttca ttctacacgt gtttttttaa aataaaagac tagattttgt agttttttag
    56521 tggtatatag gatagacgcc actatatata gtatttagaa aaaaatcatt tttcagccat
    56581 tttatagata aaacaaaaat aaataacgtg ttttcatcga tatgaaatga cttacttata
    56641 gaagatactg ggatttcgtc ctatatgcca ataacgtgta ttattttaaa caacaaatac
    56701 ttgtttcttt tattaacgaa aaaatcattt tgttctcgtg ttatgtttca ctgataaatg
    56761 atgtttctct aaataaaaaa gccgacgaac taaacgatca aaatttgatc atttttgttc
    56821 atcagcttct cctaaaaatc tttttgttaa cgataaatag ctggatttgt aaaaatatca
    56881 cttgaatcgt cactgctgga agaaaattca gtgataaccg cacctgttgc gcctgcctgg
    56941 aactagtgtt tgatattggg ctcgaacgtg tactgatctc tagcatgaag aataatttct
    57001 ttttttactg tataataact ttctttcctt tttggaatag tacaggaaag tttttttgtt
    57061 tcctttcctt cgacataagg tataccagac cctttcgaca acgaaaggtt ctcatttttc
    57121 cttcatcgtc atttcaagga gggtgccgat gctctggaca gtttcgatcg ctcctaaacc
    57181 aaaatctgta tattcgattc tttctaactc tgattctttc ataccattcc tgtctcaagg
    57241 tacatctatt gtactttttt cttgtaatcc attctgcctc tcctaactaa ttgtcaaatc
    57301 atcccaagat atgtcttctt cctctttttc ttcttggatg atttctgcat tcggatctac
    57361 tggttttttc aataataaaa tggttattcc tgtgacaaaa gaaccagcta acaatgcacc
    57421 aatgaatgcc catggtctgg tcatcgtagc gactgcaaac attcccgatg ccggaatacg
    57481 gctatctgcg ccccaaaaca tgcttaatcc gccacctact gtcccaccga ctcccgttgc
    57541 tatgacattg cgtgccaagt cgttcaatac gatagggaaa catccctctg tgatctgaca
    57601 gatccccata ggaaaagcgg tcttcaatgt atcgatttct tgacgattga gtatattttt
    57661 acgaatgatt ttccctaaga aataagcgat cgtcaagccg aatggggtta ccatcgaagc
    57721 taaaatcaat acagtgattg gttcatagat tccttctgaa aatcgtgcca caccccctta
    57781 tccagaataa atggtaatcc agaaacagcc gtgtatctgc ggtaataagc ctaaccaaac
    57841 gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgca gggcagcttt
    57901 ttcacaacct ctttttttat ttatgtttca taatcaatat ggtaaggatg cagctccgta
    57961 tggtagaccg cctccgaata ttcaatcatt tccccaattt gcgtagacga tttccgtatc
    58021 cgtttgatgc cgatctgtgc gtctgttttc agaattcctt gttcttcttc tgttaaaaat
    58081 actgccgaga aatcatcaga aaatgagtca atagtatatc cttttttcgt cagcaaacga
    58141 tacaaagatt ctttgccaaa ttcttctctg ctgaattgca tcatttcttg tggaagataa
    58201 tgaacaaaat aaatataagg ctgattgttc aaataatata agcgcttaaa acaaacagct
    58261 tgctcaccga aaaaggaaaa caaaggatct tccttcttta tatggaccaa ttgcaaatcc
    58321 agcgtttctt tccggacttc atatcgggag ttctccaaga tttgcgtaaa gcttgccgct
    58381 ttggataatc gattgtaagg acgattgctt agaaccgtcg tcccacgacc acttttcttt
    58441 gcgacatatt catctgatgc cagcagttcg attgcctttc ggacagtgat tttgctgact
    58501 tgaaacagtt cttctaattc actttctgtc ggcaacatcg aaccgactgg atattttcct
    58561 gagaaaatat cttctttaat tttatctgcg acttccaagt atagtacacg tgtccttttc
    58621 ataacgtcac cctttgcttt actattacta aaagaatagc acataaaaaa taaaaaaaga
    58681 acacaaatca catgtgagaa gtgtgttctt tttcattcag ctgctttaat tacaagttta
    58741 cgccatttga ggcaattact tctttatacc aatcaaaaga ttttttcttt gatcgattca
    58801 gtgtcccatt tccttcatcg tctagatcca catagataaa gccgtaacgt ttactcattt
    58861 ctcctgttga tgcactgaca agatcgatac atccccaagg cgtatagccc atcaaatcta
    58921 caccgtctgc tactgcatta gccatcgctt cgatatgacg acgtaaataa tcgatccgat
    58981 aatcatcaat aatcgtgcca tcctcttcca tatgatcaat ggctccaaga ccgttttcaa
    59041 caatgaataa tggtttctgg taacgattgt ataattcatt caatgcgatt ctcagccctt
    59101 ctggatcgat ctgccagccc cattcacttg cctcaagaaa cggatttttt actcctccca
    59161 gcaagttccc ttctgaagtt gctgcttgtg gatcagtctc attgattgcc gtagacatat
    59221 aataactaaa tccgatgtag tccactggat attcttttag caactggaag tctttttccg
    59281 tttgctctaa tttttccgga tcgactccat attttttatg ggtacgcgct gtgtatgcag
    59341 ggtattctcc acgtacttgg acatccgtgc agaaaaaatt gaattcttgg ttttggatca
    59401 ttgttgctgc ctgattgact ggattggcat cgatgctgta agttgttgca tagataatca
    59461 tgcagccgat ttggatatcc gaacgcaatt cgtggccgat ttttacggct ttgctacttg
    59521 caacgaattg attatgccat gcttggaaaa tattttggta ctctcctgct ccattgcttt
    59581 ttactaatcc ttgacttaaa gctggaaagt gaaaggccga attgatttca ttgaaagtca
    59641 tccaatattt gactttactg ctgtatctct ctaatactgt ctgagcaaat cgttcataga
    59701 agtcgatcag ttttcgattt ttccagccac cgtattcttt tgccaaatga agaggcattt
    59761 cgtaatgaga aatcgtgatc acaggttcaa tatcatattt taagcactca tcaatcagct
    59821 gatcataaaa ttctaatccc gcttcgttag gagtaccttc gtctccattt ggaaaaattc
    59881 gtgtccaggc aatcgaaaaa cgataacatt tgaatcccat ttccgcaaac aaggcaagat
    59941 cttctttgaa acgatcgtaa tgatcgattc ctcgatgatt cgggtaaata tatttttctt
    60001 gatcaatcgt ccagtcaaat tcttcactgc cgataatcgc aaagcgctgc ttacctccag
    60061 gcatagcatc tgcaacggat agtccttttc cgtctcgatc ataagcccct tctaattgat
    60121 ttggcagcag ttgctccacc ccataaaaat ttttctgaaa aacgtgattg catcctgatt
    60181 tcctccttat tttacaattg ccagaacatc ttcgtttgca ataatctctt tttggtctaa
    60241 ttcaagtata tcggcaaatg catcagtatt cgtaatgaca actggagtga ttacgctata
    60301 gccagctgct ctaattgctt cgatgtcaaa acgaatcaat aaatctcctg ctttgacgag
    60361 atccccttgt ttgactaaga tctcaaagcc attcccattt aattctactg tatccatccc
    60421 gatatgaatc aaaatctccg ctccgtcagt cgttgtgatc cctacagcat gtccagttgg
    60481 aaaaatagtc gtgatctctc cgtctgcagg tgcaactagt tctcctactt ctggatcaat
    60541 agcgatacct tttcccatca cgccagaaga aaaaacttgg tctggtactt cactcaatgg
    60601 cagtacttta cccgttaaag gactttttaa ggtatgccgt gctgaagtaa tcggttctcc
    60661 tgcattcgca tgtttgtttt ctaattgatt gtcttgtgtt tgttcgtcaa atcctaaaat
    60721 caatgtacct acgaatgcaa gaacaaaagc aattcctgta gctattactg ctactgtcac
    60781 atttgactct acaccatcca ccgtattaat gaaagtcgga atcgtcaata agctgaccaa
    60841 actgctggag aaagctttta ctccggaaaa tccgataata gctccaccaa tacctccaga
    60901 aatacaagcg gcaatgaatg gtttcttcaa aggtaatgtc acaccataca cagttggttc
    60961 agtgattcca aataacgaag tcactgtaga agaaatccct aatgctcgaa gtttcgtatc
    61021 ttttgttcgc aatgctacag ctaaagcagc cccaccttgt gccaagatgg ctggaagaag
    61081 cattggcatc aatacatcaa acccatattg ttcgatattc aaaaacatga tcggtacgaa
    61141 tccccaatgc atgccgaaca tcacgaatac ttgccataaa cttcccatga ttagacctgc
    61201 tacgattgga ctaaatccat aaatcgagtt gaagagaccg cctaaagcat taccggcaac
    61261 cgtcccgatt ggtcctaaag caagaaatgt taatggaacc atcactacta aaacgacaag
    61321 cggaactaaa atcatttgca gaaattgcgg aatcactttt tttacgaatg gctctaattt
    61381 actttgaacc catacggcta gaatgattgg tatgacagaa gaagtataac cagatggtga
    61441 aagaatcact gggatcccaa agaaactgat agcttctccc gcaccagcaa ctgctgtgat
    61501 agctggatac acaagtgcca tagcaattgc tactgctaaa aactggttgg ctttgaactt
    61561 tttagctgct gtaaaagcaa gcatgacagg taagaaggta aagacaccat ctgctgcagc
    61621 aaacaatatt tgataagtac cgctatcagc tgtcagccag cccatcaccg ttgccaatga
    61681 taagaatcct ttcaataccc cggcggctgc catcgcccct agaaaaggtg taaatatact
    61741 agagataata tcgatcagac gattcaaaat atttttggac tcttcttttc catcctccga
    61801 aactgcaaga ccttcctcat ccacgatcga ttgataaaca tctgctacat tactgccaat
    61861 cactacttga tactgaccgc cactttgtac gacttggatc acgtccgggt cttcttttaa
    61921 cttttgcgta tctgctaaag attcatcttt taatctgaat cgcaaccgcg tagcacaatg
    61981 tactaaactt ttgacattct tttgtcctcc taccgcttcc catacacgac gaccaactgc
    62041 ttgattatcc attgtttttt ccttctttct tttttattta aaaaataaaa acccagattg
    62101 aaaaaaacag tcacaaacat tcgtgaacct attttttcaa tccaggtttt gcctgccata
    62161 cagtaacaat ccttatgatt ggatgctttt tttctcactt actactcttt gaatatgaat
    62221 cgttaaataa atctgctctg cgatccccat ttcctgtttt tgcgtttgtc gaagatactc
    62281 attgatccgt tcgacacaat gaaacgcttt tggatacttt ccttgaacta atgcgtagag
    62341 aaattcatct tgctcgtcag atccttcccc tttgagataa cgctgggcaa aaaactgcag
    62401 atgagtaatg atacgctgat aattcaaaga ttcttcatcg aacacgatgc cgaagaattt
    62461 actgatgata ctcagtatat ctcttaccat tgtggtagcg gtcatcgtca cttgcatgtc
    62521 ttcgtttcct tgttggctat tcacaaaatg aaaggcaata aaaccggctt catcctcaga
    62581 aagttccaca cccgtctttt gtttcacgaa caaaagagcc tctttcgctg cttgatattc
    62641 tttcggataa aatttttttg tttcaaataa taatggatta ggcagttgga tattttcttt
    62701 tgctcgtgtg atcgcataat gaagatgatc ggtcaatgtc agataaatat taggcgaaaa
    62761 ttcaactttt agtttatctt cagccagttc gactacttgt ttgacccatt cgatctcctc
    62821 tgccggaata tcagccatca gctgctggaa ttggttttta gcaaaagaat ctcttaaaac
    62881 aaattctttt tggaccgctt ctggcgaaat ctcgtcccca atcttttttt gaaaagctag
    62941 tccttttccc atacaaacga cttcttcccc gaatccattt ttcgaaatga cgacattgtt
    63001 attcaaaatt ttatcaattt tcattttgct tcgccctctc aagaaaaata aaaaaaccta
    63061 gaattcgcct actgtttctt agctgaattc tggttttgcc tgccgtacag taacaatcca
    63121 tttcttattc aaaatcatca taccatcgaa tttttattta tgcaagcctt ttcatataaa
    63181 aaataaaaaa actggaacac aagtctatct agaaaacaac caaatcagag atgcacctct
    63241 ttgcgtttct ctttttttca gcaaattggt ttttccttat cgattcatat cctttggctg
    63301 tttttaatct gtaacgactt atgtcccagt atcctaaaat gtttttgtct ctagattgaa
    63361 agcagatctc atatcatctg tcgcatcttt tatcgatgta acactttcca gttctccttg
    63421 tacaactagt cgcgtgacgg cgtccatgtc gccacaagta attagtgtga caatttcttt
    63481 tccctcgact tcattcagta attgggtatc tgtaggagca actttttctt taaaaaatgt
    63541 tttgtatgta tacacatttt ctaaatctgt caaatagatc ttggcaccca tctctaaatt
    63601 ttctagcggt gtaaacagca attcagggtt cgttgcacga tggcttgcta aggcataatt
    63661 tccttttccc atttcttgat ctggagataa tgttcctgct ccgtaaagta gtgacgtatt
    63721 atccaagcct ttgaaaaccg gtaaattgat tgaaactgaa gggactgcga tactagcaat
    63781 cactggtaga tctgttcctc tcatttgcga cctcatgacc ccttctgtcg tcattggttc
    63841 taccgcatca aagtcaaaac tgacatcttt atcgttattc tttttcagat cttcttttgt
    63901 tacattcgct atagcatatt tatctcctgt ctctctgaca aaataatcct tgatctgttc
    63961 attgaaaatc aaagctagtc ctactaataa caagaggaac aagaggatat tgactagcca
    64021 tctttttgct tttccttttt ttttcatttt ctctccctcc agactcattt tactgttttt
    64081 cttttgctct ttccaacagc cgaatgacac atttctacaa ctctgtgtat ttttttcgta
    64141 cacctgcgct tttttctaca ggatatttca aattattttt gatcaatttt tcttcaatga
    64201 tctcatctag atccaggtga aggttgtctg ccatcatgta tgaatagatc aatacatctg
    64261 ccagttcttc tttgattcgt tctaattgtg tttcttttac ttcttccggc gttttccatt
    64321 gaaaaagttc taacaattca gatgcttcca aagaaataga gatagccaaa tctttttcgt
    64381 tatgaaattg tcgccaatta cgttcatctc taaactggtt gactttttcc atactattca
    64441 tttgcttgcc ttcttccaat cacttcttac ttgattaatc ataccatttt tttacataaa
    64501 aaatagatga tgcttttaaa aaaactcctg ttttctttta tttaaaataa atgtcataga
    64561 aaatcaataa tttaggtagc caccatgtgt atgcagtagc gtgtcttctt cccttgaggc
    64621 tttttgtttt tcgatacgtt cttcttccca aacagacaac gctactagaa tctctcttaa
    64681 tgattttcct tcttcggtga gcgagtactc tactttaggc gggacttggt tataggcttt
    64741 tcgatggata ctttcatctt gttctaactc tcttaactgc tgggtcagca ttttttttgt
    64801 gatcgtaggg atttgcttgc ttctccacgc ctaaaataag cgatattgct cttcccttta
    64861 tctggtgtaa gcaaagcagt tagcattttg acgatcgtgg gttccgcttt tcacttctta
    64921 tctacttagt atcatgaagt atactttcgt actaatacta agtagcggaa gtgtcaacta
    64981 ctttgtttaa ctacatagca aaagaaacag aagggtgaca acggtcatca cgcttctgtt
    65041 tcttttgaat atgctgtgtt gttgtctcta tctccgtgta gactttcctc aaacttatcc
    65101 tttgacagta agtcagtagg ctgatttgct ctttctagat gccagataca tttctacaaa
    65161 aaaatagtga ttctgttttg aaaattcgat ttttagttct gcattttcgt aaacatccaa
    65221 catttttttc gcactgtata gacctaaccc gccatttttt ttagaggttt ttcctctttt
    65281 gaccatctcc gatagcgtca aattcgttgg atttgccttg ttcgctatac taaaacgtga
    65341 tccctctttt tcttccagca gattgatcgt aatcggactc tgatcggcta cacgatgatc
    65401 gaatgcgtta gaaataaaaa tagataaaca gcgaataaaa acaaccagat caatcgggat
    65461 tgttttcact tcgacaggaa taactacttg gatcgggatt ttctccgatt ctgctttttc
    65521 tagaaatttt aaaaagacac ccctgacagc tggggataca atatttgtta gctgttgtat
    65581 ttgcggtttt tcaaaaatag ctgcagaatg atcttctaat atttctaaaa attccaacgt
    65641 ttctttttta ttttcctgaa gagtaatttt taatgaaaac agtagacttt tataatcatg
    65701 tttgaattcc tctagatgat ggtaatattc ttcatttatt tgatacagtt gaagcgattc
    65761 ctgatgttgt ctttctttta cttggaaact gatcaacata atggaaaaaa acaaagaaaa
    65821 aaaactgatg actatcgaca aaagagatag aagcagataa ttagatgagc ggaagtgaac
    65881 acttcccaat accaagtaaa tcacgcccca gcttaataaa atacaaatac ctccaaaata
    65941 gtgttcagtt ggataagtaa aaagcaagtt gagaaatctc gtctgtttgt ctatactttt
    66001 tactaataga tagaaaataa atgcgataag tagtgcaaaa aaacttgctg ttccaagatg
    66061 tctctttcct aaataatcca tcagatcaaa aaccagaaaa aagctgagtc cagtgatagc
    66121 aaaaaaaaga ttactgatca aacagctgat aaacaggttt ttcgtagtaa aataaaaaag
    66181 aaacatcaat aataagccaa ctaggtaagg aaagctcact ttagggatag gaagtaaaag
    66241 tgtcaccacc atcaaagcta tacaaccaaa gctgtttttt actagcagtt gtttcgaaaa
    66301 gaataaatag atacctaaat ctaaaatcaa agtaaaggtc aaataaagtg tcactgttgc
    66361 gaccatctta ttcctccatt gttttgtttc aaaatgtaca catatttttt taataggagc
    66421 ctgcatatga aaaagtattt tatcatagaa gataaccagt ttcacagcga atggatcatc
    66481 agagaattgg tcaatcatca tatcgagaat atcgtgcagc tcacttctat ctctcaaatc
    66541 aatcagttga tccagttggg cactattgat aatcacgatg ttttctttat cgatatcgac
    66601 ttgaagcaat cactcacagg tattgatgtc gccgaacaaa tacgtaaaaa aaatgcacat
    66661 agtaatattg tttttttcac cgcttatacg aattttgcag tagagatcat caatcggaaa
    66721 atccttccga ttggatatat cagaaaagac aggaatttat ctgaacaaat tcgtcaagtg
    66781 ctagaacaga tcaattggaa agaacagcag atcgtaaatg tcgataaaat ctttattgat
    66841 caaaaaggtg gaagtctcgt cctgtatcct cgggaaattt tatatattga aagtatcaaa
    66901 ggtttgaaaa accaagttat ggtcaaaaca actacagaag ataaattagt gaatacttct
    66961 ttaaaacaat tgaagacaga gttagaaaag tatgcttact ttttactttt aaaatcttac
    67021 tgtatcaact tatcgcaatt gcagaaaatt gattctgctc attatgtgtt agaatttttc
    67081 aacggtgact ccttactcgt tggtcgaaag atctttgaaa agacaaagga acgtttccat
    67141 gacttccaaa aaactgcttc gagttgatga ataagagctg agacgtaagc atagcagatt
    67201 tcagggagtg ggccttgaat cggctccact ccctgaaatc gttgatatga ttttacgctt
    67261 cctcttctgt aggagcatct gttaacgacc atgtgaatgt tgaaacgtag ttgtctacag
    67321 cataattgga tacaggaaca aataattcca ctgattgtct cccttgttcc acattctctc
    67381 ctaatgaata aatccatgtg ccaatccctt catttgctcc tgcagttact agatctactg
    67441 cctgatctgc tgggatttcg attccttcag cgccagttcc agaaacggta gatggcattc
    67501 cactgctatt agttgcgcta tttcgaatca cggcgttttt gaatttcacc tttgaaccag
    67561 ttagtttatt cgtatcagat gttgtaccaa taaattctgt tcgtttgact gataatttcc
    67621 aaccggcata atttccgctt ttatccgtta cttgtacgaa attctgtact tgtgtttgtg
    67681 ctccagtact gtccgttccg ctttgtaatg cagcatatac tgtttttgct gtacctaact
    67741 tgattgttcc aaaatcaaga tctggagctt ggtcaatcgt cagtaatccg gatgttccct
    67801 gatttggttc gtcaggtgta aaaggagtat caggatcatt tggatcaaca ggaggaactg
    67861 gaacaacgtc ttcaggattt gcagcatctg gtttggtcac agaaatcgtt ccttctgtag
    67921 tggctgagct cttattctca gcaaaaacac tttgtgtaca taataataga cttccagcta
    67981 gaacggtact taatgcaatt gtttttttca tttctttttc cttctttctt ttaaaataaa
    68041 taatccagca aataagccaa taagaatgac tccaacaagt atcagccatc gccagtcaaa
    68101 tgtttctggc tgatttttta aatcaatatc cttttcattc aattggtccg ctgcatcatc
    68161 agttatttca aaatcggtat ggagattcca gcgtttttct ttccaagcac ccttcacaac
    68221 cgcttgataa tgtcccggag ctaatttttt tccttctaat gggacaagta aatcaaaatt
    68281 tgtattgggt gctacatcta ttttttcttt ttttattgaa aggtaaggct cttttgtttc
    68341 ttttttgtag atttcaaccg atgtagtcaa gccgaacata taagaagcat ttggattttg
    68401 aagattcgcc ttaatcacat ttcttccatc gctttgatcc gcataagctt tttttaattt
    68461 caaatctggg gaagactctt tttcgctatt tcaaatcaag attgctttca cttgagaaaa
    68521 ctgattgacg acaccaactg tacttttgtt agaaacgttc tctttttttt cttgaaacgt
    68581 gatccctcct gcgaccacac cagccagctc tttttctggc gctttcagtg aaagtgtctc
    68641 agtgaccgca ctttgaggag gaacagttat ttctcccact gtttttgcta agtttttcat
    68701 atcataactt aatgtttcat caaatttact ttttgattct ccatattcga tcacaccgtt
    68761 tgaattcgtc gtagcggagt ggatcaatgt ctcaactgtt actgatttat ctgttcggtt
    68821 aaagaccgtg taggggattt cctgtgtttt cccaggctcc atccttacat ttatatatgt
    68881 tttgttctta tcaatctgat tagccggtaa ttcaaaatct accgcaaaat tgaattcgga
    68941 ggcatggaca ggtgtctgcc aaaggatgaa tccaaggaga aggatcataa gtaaagagca
    69001 gttgtttcga tgacttttat tccatattcg attcatcaat catcctcctt gatctttatt
    69061 ctgtcggcgc atctgctaat gtccatgtta tctctgctga gtaattacca gctatctgat
    69121 tttctttggt taagacaagt ttaaacgccc gaccgttatt taaatattct gaataatctg
    69181 cttgcacatt cgttgaattt tgtcctctga caatttccag ttcttcttca ttcactaatt
    69241 gatactcctc tcctttcttg aaatatagga catcctgcat agaaaatcct gcatcatttg
    69301 tgaaaggctg agataatttt atcgttaaga tccacggatt tttcgtttct cgctgatcat
    69361 aaatccataa tttttcctgt atatccattt ggccaaatgc ttcttgtgtc aaaccagttg
    69421 ctttgatctg accaaaatcg agtttggaag gggcaagaaa tgagagacca ccttctacta
    69481 cgatcttcgt agcaggtgca aaaggcgggg catcatgata aacaactggc gtatcacttg
    69541 gattgtgatt cccaatttca tcgttggtac ttggcttgtt ctgcacaccc ttagcagtag
    69601 cttctcctgc ttggtcgttt aatacaattt ctaatacatc tcctgcaaaa agttgatttt
    69661 ctgggataga aagaacaaat tcccctgtat catcaatagt tgtttctgac aaaacagctt
    69721 gatttttttt caatgaaacc aaggctcctg cttcacctgt acctgtgatc gtgcgagaag
    69781 ctgcattgat caatgtggta cttaaattag caggttcagg aggaatcaca tcgactgtcg
    69841 ttttggcaga gtccagttct gtcacttctt ggccacctct tactttttta gcagcaacaa
    69901 cttgcacttg atcctccgtt tttaatctct catcattggg tacttgaact tcgaacaagc
    69961 cgccttgttc gccttctccc catgggttat atcctgcccc ttcattttca tctgtttgtc
    70021 cttgcgtgag agcagtaatt tctacagatg atttcgtcgt atctgcgtaa gtaatacgta
    70081 aagtcacttc tacttcgcct gtccaagcat ctcttggctt tgcctcgaca ccttcgggaa
    70141 caacgacatg cccatagatt tttttatctg cattcgttgg cactcttaag gattcaatca
    70201 tcgggagctg attattggca ctgattcgat tataattttt cattccggca cctgtctgag
    70261 caaattcatt tgttaaacct gcactagggc ttgatacgaa attgcctaag tcggtcccat
    70321 tgatcgataa gtctgtcata tcagaaaaat aatccgggtc tttttcaaag ttatcgaatg
    70381 ttgcgcctgt ttttcgccat aaagcgattt ctgaatgggc gatctttaaa gaagaatctc
    70441 catttccttg gaatacccag ccgcctatgc cattggtctc gcttccaatc cgtttgtttt
    70501 ggaaatcaaa ataggtagga gattctaagt ccacattcaa cacatcccca ctgatgatgc
    70561 cggtcttatc ttcccctgtt ttcccgacca tgactaaata agttttttca cctgccgtaa
    70621 aatttaaatc aatatctcca gttgtatcta cagttgctcc ggaatctgcg cgtatatcga
    70681 tatttgattt ttctccagtg actgtaaaat cagctgaacc tggactttct agaaaaccag
    70741 aagcgtcata aaattggatc gcttgatttc cttgattgag tctgttcacg taagatttgc
    70801 catcacctgt atttcttacc ctcaatgagc ctccattggt tactgtcagc ttttgcgtac
    70861 cattctcaaa acgaactgcg ctggaacgcc cactctgctt atcgatgtcg ataactgatc
    70921 cggctccttc tacgatcaaa gaaaggcctt ttcctacaaa acgcatccca ttgttcccgg
    70981 gttgatcgtc atctccagcc attttgaccg agatttgtcc accacttgat acatggattt
    71041 gactagaatc tccttgcaag cgaaatccgc ccgtagttgc tgtattgatg gtgattttcc
    71101 caccattcga tacagtgact ttcgatcctg aacctctcat ttggatgatt ccgcgattgt
    71161 tgtcattggt aatattgatc tttgtattta ccgaaaattc gcttcccgaa ccactgactt
    71221 caaacatgac attaccaggt agcgtatcga ctgcctctcc ttcatcgcta acgccatctg
    71281 cccgatcata atcaagagag acctttgaac cgcttaagat atggattcct ggcgctgttt
    71341 ttccactacc agtctcatct cctgaatcta gaaaaccgta gaatttccct agatacggtc
    71401 cataaagttg actaccattg atcacattca attgatagct tctacaaaaa taggctttac
    71461 ccggagcggc attcagtcct tccttcactg gttggacatc caacacaccg gaaacaccat
    71521 ctaatgtgac agttgcacga ggagcgtaaa tcagtcctaa tttggaccca tcatacaaat
    71581 caagtgtccc ggtaaatttt atttctcccg taaaatgata tctgcttccc caaaaatcgg
    71641 tcatataagc aatgataatg gcatttccat cgatatttgg tgtcaatcca cctggatgtg
    71701 cagctgttcc tttaaaattc tggaactcga tcgactgagg tatctcattg atataaatat
    71761 ggtgtgtgcc gaaattgtga gcgaatccac caaaatcaat aacgatatct cgtttgatct
    71821 ctaattccct gtctaaagtc aaaggctgaa tcagtcggat cttagttact gattcgtctg
    71881 cgagaagctg cgccagttct tctgttgtgg cagcttcgct gtacccatcc gctgaaacag
    71941 ctcttgccag tgccgcttca ctgctatcag acgtttgttc agctacttct gaggtctcac
    72001 ttgtcttatt ttcttcttgc cttttattag tagaagcctc actttgagat tctgtagttt
    72061 cagaagtaat cgattcttct gaaaaagtag tcttgtcaaa actttgattc tctgattgcg
    72121 ttgattcgtt tgttttctct acatcggcaa aaacggatgt caccatcaag agagggaaga
    72181 caaacaaaat cgacgcaatc agctttttta cacattgatt cattctccac catcctctct
    72241 gacgttttga caattttatt ttatcgtttt aatctatttc caattgtact ttcatattta
    72301 aaatatgtat ttttatggaa aaatttttct attaggtgta aatttctttt ttgtacaaaa
    72361 aaaaggctgt gacaaaagtc ttgtcacagt cccttatccc gaacaagcgg tgttctcccg
    72421 ccaactcctc ggtcacaacc tccattttaa caataaacct gtaaatgtga gggcaatact
    72481 ttgattgtca caggtaattt atccccttca tctccatcaa cattcgtcgt caattcttcc
    72541 tcatcagcta atgacacaac taattgctca caagtaaggt aggctagatt tttcgtagat
    72601 tcatctactc cttttaatag gtctggcaat gttttaagcg tatcgatcaa agaagaatct
    72661 tttaaataaa ccaaatggag cttaccatca tccacttttg catttggaag taatgtttca
    72721 aaaccgccga ctgagttagt tagtcctatc agcaaggtac tgctctcaat ttcttccttc
    72781 tttccatcaa cttctacgtg aaacgagtag gattgtgtgg aagctaactg tttgattcct
    72841 gacatgaaat aagctagttt tccaaatttc gtcttcttct ccgcatctac atcattgatt
    72901 gattcaggga tagagccaat tgcgacgaca ttcataaaat agtcgtcatt tatttttcca
    72961 atatccaatg gcttgactga ttcaatcgaa aaatgattga tcgcttcctc aggttctagc
    73021 ggtatcccta atgctcgagc caagtcattc accgtaccca atgggaaaaa tccgaaattt
    73081 ggccgatgtt cttgttcagc gattccactg attccttcgt tgactgttcc atctccgccc
    73141 atgacaaaca cactatgata cccttccgta gccgcttcgc gagtaaagtt ttttgcatcc
    73201 cctgcttttt ttgtatgcaa gacgaccact tcatcaaaca ccgattccaa ttttgctatc
    73261 gctaattgct caaattcctt tgcttgttca ccgcctgaac tggggtttac cactaataaa
    73321 acttttttca tcactttctt tcccttctga ttgatttctt taatataacg atagcataaa
    73381 gaaaaaaatt aggctgtgat gaagtactgt tcatgaaaca ttcatttatt ttttgacaag
    73441 ataaaacatc catactatga aactgaggaa aattaaaaca atgatatagg aaataaaaga
    73501 aaatgatagg cattgtatac ctaataaagt aaaaatctta ggtggcagat aaaaacacaa
    73561 aaatagcaag agaatgtaat agcgtttctt ccactttttg ttatcagacc ttatagcgat
    73621 cagaggaata ccaaatgcat aactgaaaaa ctcttgatgt actgttaata tgtcacggcc
    73681 ttgaataata aatgcgacaa tccccgctat aattagtgtg cctaacacca aaacggaaaa
    73741 ttgatgaata tattttatat acaaatgatc cattgtactt cctgcctttt tatttgtgag
    73801 aaaaacaaat ccgtatacgt gaataacttc tagaaataaa agaaatttgc aaaaccactc
    73861 catttatcta cttctacgtg cattttttga tattgttttc tttatttaag attatacaga
    73921 aaaatatcta gggtcgctgg attctaagta aaaaaaattt ttaaaataaa aagaactaaa
    73981 tcaagtagta attaaactac tgttgattta gttctaataa aaagatttag atgaacagtt
    74041 ttattgattt aaatagttca tattttttat ttataccgct tatttactac ttgctgtact
    74101 agaatctgaa gtgaatgtac tcaaaacatt ttcaaagtct ttgtctttga tttttacatt
    74161 agcgtctttc aatacttcgc caattacttt attctggaag gtactgtcac ttaattttgt
    74221 gtctgttgca atttctttca attctttttt gtatttatcc atgtcgttgc ctttgttttg
    74281 gtttttcacc attttaacca cataatattc cgtagtataa gttgaagcat tggtagaagt
    74341 gatcacatca gaaacttgac catctttcaa cttaaaggct gcttctttga cttctgccgg
    74401 aaccgttgta gtagtggaat caaatttcac ttttccgcca tcttcttttg tcgtagtatc
    74461 agttgacttg tctttggcta atttagagaa atcgtctccc ttatcagcag ctttcttcac
    74521 atcttttgca tcatcttcac ttgctacttg gataatttga gcttccactt ctggatggaa
    74581 agattcccaa gccgttttta aatcttcatc ggtcaatttg atatgtttct ttagaccttc
    74641 ttgaaaggct agactttgtt tgatactgtc tttgaatgtt tgttctgtgt aacctgcaga
    74701 tttcaattgt gaatcaaatg aatcacccaa ttgtttcttt gtttgatcat attgagaatc
    74761 gatttctttg gttgatacgt catctccata tttatcttca aatacttgat aaacgatcaa
    74821 ctgttgcaca gcggaatctg ctccttgcat ttttttcaag ctatccatta cttcttgttc
    74881 gctgattgta ccgcctttat aactaacggc tgttttttca ccggcattcg tacagcctga
    74941 caataagcca gtgcctagta cgatggcaag tgctaaaaat ctttttttca tttatcaatt
    75001 ctcctacttt tcgttttttg cgaattgcta aaaacaaagg tgttcaagat gaaaatcatc
    75061 aagaacacct ttactaatct gctcgatacg tcctgaatag atataaggct acacaatcca
    75121 gaaagttcgc tcagcagaat aaatgtcaag tagcacatca caccttaata tactacttct
    75181 tttgttaccg tttttcaagt ctatcttgcg ttccgtattt aacgtaacta attcttagaa
    75241 caaatgttat cgattttgtt tttcttcttc cgctgctggg atattcaaat cagacacttt
    75301 tatgttcgat tttccagaca tttttttgtt gtttttatta tgatatattt ttatacaact
    75361 aaaaatgaat atattaaaac taccaaaaat aacttttaac gctgtttcat tatatgtttt
    75421 ttctatttta tataaacttt tggatactta ataccaaccg gttggattgt accaacatga
    75481 gaatgtgtca gttctatttc ctgtcgcact tgcataggcc catactcctt ttgttgcatt
    75541 tgctgatcga atagagtttc caactttcgc ttttgcaccg tgacgattat agacacgact
    75601 atagtggtta gaatgacctg tttttctcgt acaagttgta tcactataat aaacccatct
    75661 tccagagcta tgattaattg ctgttctaga aatttcccgg tctactggta aatttgactt
    75721 aataatatta cctttagcat caaaatgaat ctctcctggc tttaaagatt tgtaatagat
    75781 ttcaccgtga actgaatttg cttccacttc agatgacata gcgtttcctc caacaaaacc
    75841 tatccctagt gcacaaataa taactgcttt tttcataaaa cgacatcctt tcctttttga
    75901 tttttaaagc atgatcttct tgtttcctat tttggtcaga tatggatcat gcgtgactac
    75961 gataactaac gtaccttggg aagcttgttg cgataagatt tcaactactt tatccttatt
    76021 tcttttatcc aatgacccag tcggttcatc ggctaaaatg attttgctat tcttcaataa
    76081 gactctcgca caggcgattc tttgcttctc accaccagat aatgtgtaga taggcgtact
    76141 tagcttcttg tttatattta cttttttcaa tgcctcatac tttgctgttc gcttatctga
    76201 aacatttaaa ttcgaaaact tcaatccaat atctaaatta aaatcgattg tttcatattc
    76261 aatcaaccca aaatcttgaa aaagaaaaga tatggtattt ctttttatca agcgcttctc
    76321 tctcccagtt aattgtttcg tctcttgatc gaacaaatag aattgtcctg aactgaactg
    76381 atcattcaat cctaatatat tcaataacgt acttttacct gctccacttt ctccaacaac
    76441 agtcacaaga tccccagcat ccgcagtgaa acgatccaaa tgaagaatcg ttttgtttcg
    76501 aatgattttt ttcgcacctt ttatcactat caacggctcc aattgttaac ctccattcat
    76561 cagcaataac tgacttttct tttctgtaga caaaataaaa acaactgaaa gaataaaatc
    76621 aatgaagcta aagaaaaaaa tattaaaaag atatttaggc tctctttcta taagaaaaag
    76681 gaagattaca cttgtcgttc cccatttgat gaggttcatc atgaagaaat ctttatacgt
    76741 ttcaaagagc gaataaccac ttgaacgaaa gatagcgatt ttccgacaat tgtagacaaa
    76801 atatatgcat aacatgacat agctcaacaa gaagaaagaa aagaaagtga gtaaaaatat
    76861 ctgtatcaag taatgaaatg atctgcttaa cactttgaca gctatctctt cagtctgagt
    76921 atacggaaat gataactgca agttatcacg taactgactt tcttcaatca gctcaaataa
    76981 cctttctttc gacaaagagc ctatcttgat ctttaacggt ggatagattt ctccgctgag
    77041 aatatttctt tccaaataca tactgttttt caatgtcagt atttcaacaa ccggataatg
    77101 ttcgatccag gggatagaag gaataaatgt ataaatgggt tgattgtttt ttataaatag
    77161 aaatttcggt tctttttcac tgatttcttg ataatattct gttatttgcg ccaggtcatt
    77221 ttttaaatct gtatgtctat atcgttcagg gatcaatata acagggtgcg tttctttttc
    77281 ggttatttgg atcaaccttc cagagacatc aatcaaagga aatttcttaa gataattcag
    77341 atttattttt atccctctgc caaactgttc attttcttca ttcaagtaat ctgatacatt
    77401 gactaatata cttccgtttt tattcaaagt ttcatataat tcctgatctg ctgctgatgc
    77461 aaaacgattg gaataaataa aatccacgtg gtttttcccg atggttactg gataaaatac
    77521 atgatagtcc tcaccagcga tttcttttgg tggttcatgg gaaacagcta actgtgaaga
    77581 agaaaaaacg atagttgcta attcgacatt ggcaacggtc aaaagaagag aaaacaacaa
    77641 tccaaatgta aaaagaatgt attggatata gttgaacgct tctcttaacg aagcgatttt
    77701 ttcaactaat aaaagagaaa tcaaagagat accatatatt ccgctgaaca agaaaacgta
    77761 catctggaac aaataaagaa attgctcctt ataatacata aaaataaaac caccaatgat
    77821 tcctgcaatt atgaatgtaa taatgatttt ctttttcccc aataaacacc acaatacttg
    77881 atgtaatgaa aagtcattat tcaacaataa actgatttct ttattcatca ataagaaaaa
    77941 ggatgataac aaaaggaata aaagaatcaa tccaatagtt aaatacactc gataattata
    78001 tacgtctgta tttgaaataa ccgaatacga atagctattt tcaaagtctt caaaaatatt
    78061 agtggcataa tcagtgtgaa atgcttgatt gaatcgatca gtgaatagtg aaatgacccg
    78121 ttcataggct tctttttctc tcgtcatcaa gaaaaattca ccttcaaaat cttttatttt
    78181 gatcgattct tcaatggacg ttacgcgaaa ggaggtatta gaatagggta ccttctcgtg
    78241 agaagatttg acatcccctt gataatacaa tgtcatttca agctttggct gtatcttgaa
    78301 gttccaagat gtatctactt cattggattc atttactgtt tttttcatat aagaaagctc
    78361 agtttctgat gagacatttt caaataccat gattagctgc tcatattctt cttgcccaat
    78421 aaaatgttgt ggcaatcgaa tcggagtata agtatctgtt tgatggaaaa attcatttgt
    78481 ccgatagagt acctgtttat ttatactaac caaggcaaaa ccactcatga taccggtcac
    78541 tatgattgtg agtaaaactg tgaacttttt ttgattatca ccactctccc ttacttaaat
    78601 gattctttga taaaatcata ccgtttttca tcaaaaaaac gtatctatca agtgtatagg
    78661 tcatgtagaa taaatctata tactgtagaa tcgaaaggag aagaagatga acaccggaga
    78721 aaaaatacga acttaccgca gaaattgtaa gcttacgcaa aaagaattag gcgaaaaaat
    78781 tggtgtttcc gataaaacga tttctagttg ggaaaattct cgtacaatgc cagaccttga
    78841 aatgctgtca cttttacatc atactttagg tctgccgatt gattttcctt ctattgaatc
    78901 agctaccgaa tcaatagaac caacattgcc agatgaacct cttgttaaca agaaatctta
    78961 ttcatcaaaa caacccttct cactcaaaaa gacactatct ttctttctgc tcgtcttttg
    79021 tttggtctat ctttttctta tcaattatca atacaaaaat gaaacgatcg atcgattcaa
    79081 tccttttttg aaaccacaaa tcggctatac cacactccct gtaggatacg tggacaattc
    79141 catcgaacgt tgggtatgta atgatatctt tggccatggt acatggctgt cttttcacgc
    79201 tggagaggta gaaaaagaga aaaaccatgc tgtaatcaaa cacaaaggaa aacacgtcat
    79261 gaacgttcgc ctgctcactt gggaagccat tccctcagat taccaagaaa tcctcagcca
    79321 agactatgac gacgccttaa aaagaagccc tcaggagcca gataggcatt aagtaggaga
    79381 tgaacgtaga gatagaaaaa atcaatgcac atgatcttat ccaaaaaaca ggggtacaaa
    79441 ttaattgaaa aagccttgag atgaaagttc ctaaataaaa aagttcagaa tgataggtat
    79501 ttctggctta tagtataata tttacgccac agcctcccaa tgattctttt tggaatcata
    79561 atcaaggatg gacattcaca aaaatatttt gctcattcga tccgaataga ttaacgacca
    79621 atctagtaag taatatagat tggtacgtag tcaagctgca cataatttag atcaatttta
    79681 gttctttttt atatttctat gatctgctca tttactttta tatgatggct tacagtaaca
    79741 taaataaata tgataatcgc tggtagttta tccaagagta ctcaataaaa tacaaaatgg
    79801 attgttcaag cgcctatcat aatgataaaa tgaaacaaat aaacgataca aggaggaaga
    79861 gactttgagt aatggcagaa caattttttc attgtaaaaa ttgttcagtt gttaatctat
    79921 gggatttcgg tagcatgggc atttttttat agaggaatca caccaacctg gccgttttat
    79981 ctcttgttag tgggaatatt agtaggaaat tttgcccctc ttcactatcg tacgaacttt
    80041 tttccaactg attctggcct ttttcttaaa acaaagacgc gaaaattaga aaacattatt
    80101 gaaatgacaa ttacctgtat aatcattttc ttagttatat tcctaaacgt cttctaataa
    80161 gaatctctta aaattagctt gttacagtta gttacctagt ttatataaca cagttatctt
    80221 atcttcctta tcaacatctt ttgccaactc tttgtcttaa attcgaaaca tataagcaga
    80281 gctacgaaca ccaagactgt ttcggctctg ttttttgcat caaattcact cttatatagc
    80341 tgataatctc ggctctttaa aagaaattat tttcgcttca ttttattata aaaaggctag
    80401 gaaccttgat gttatcaggt tcctagcctt tatttaagta ttaccccaca gacccttcca
    80461 tctcataact gatcaatcgg ttgagttcga ctgcatattc cattggaagt tctttggtga
    80521 atggttcaac aaagcccatg acgatcattt cagttgcttc agattcggtc aggcctcggc
    80581 tcatcagata gtagagttgt tcttctgaga tttttgagac tttggcttcg tgttctaaag
    80641 agacttggct gttgtggatc tcattaaatg ggatcgtatc ggatttcgac cattcgtcca
    80701 tgatgattgt gtcacattcg atatgagaga tcgaacccgc actgtttttg gcgaaggtta
    80761 cttgtcctcg atagtttact tctccgccgt cttttgagat tgatttagag acgatagagc
    80821 tagatgtatt tggtgcattg tggatcattt ttgcacccgt atcttggatt tggtttgctc
    80881 ctgcaaaggc gatggagagc atggtgcctc gtgcgccttt tccatctaag taaacacttg
    80941 ggtatttcat cgttgttttt gcacctaggt ttccgtcaat ccattcaacg gttgcgcctt
    81001 cgtatgcttt tgctcgtttg gttactaagt tgtacacatt gtctgaccag ttttggatcg
    81061 ttgtgtaacg gcaatacgca tcttttcttg taaagatttc tacgatggcg gcatgcagac
    81121 tattacttga ataagtaggt gccgtacaac cttcgacata gtggatgctt gctccttcgt
    81181 caacgatgat cagcgtacgt tcgaattgtc ccgtgttttc cgcattgatc cggaagtaag
    81241 tttgtaatgg aacatcgact ttgacgcctt ttggcacgta aataaaggtg ccacctgacc
    81301 atacggctga attcagcgct gctaatttgt tgtcagttgg cggtacaagt tttgaaaagt
    81361 actctttgaa taattcaggg tattctttca aagcagagtc agtgtcagta aagacgattc
    81421 ctaacttttc gaattcatct ttcatattat ggtacacgac ttctgattca tattgtgcgg
    81481 aagcaccggc tagataagct cgttctgctt ctgggatacc aatcttttca aatgtatctt
    81541 tgattttttc tggtacgtct tcccagtcac gagcgggttt gtcacttggt ttttggtaat
    81601 atttgatggc atcgaaatcg atgtctgaaa ggtcggggcc ccatgtttgc attggcattt
    81661 tgttaaatgt ttctagagat ttcagacgga attcaagcat ccattctggt tcgcctttga
    81721 ttcttgacat ctcacggatc acttcttctg tcaagccttc tcctgtactg aaaaccggtt
    81781 cgacatcatc gtgaaacccg aatttatatt cttctaattc tggtacgccc atgttctcac
    81841 tcctcttcct tgtggtaatg atggaaagct gcggtaccat cgttttccat tgcttttcct
    81901 agtgctttcc aggcaagtgt cgcacatttg atccgagctg gaaatttagc tacaccacca
    81961 agcatagcag catcgcctaa ttggtcggga tctttgactt cttttccttg gacaagctgt
    82021 agaaagtcta cagttaaatg ttctgcttct tctattgatt ttccgatgac ggcgtctgtc
    82081 atcatcgagg cacttgccgt actgatggag cagccactgc cgtcaaaggc gatatttttg
    82141 atgattccgt cttttacatc gacttgcaat tcgatcacat ctccacatct ggggttgttc
    82201 aattcgatct tacttgtcga gtcagtcaag cttccacgat gatgcggatg ggaagaatga
    82261 tccaagatca cttgacggta taaattgtct aatttagata gtcccatgct ggaaaaactc
    82321 ctttgttgcc atgattgctt caactaaacg gtcggcgtct tgcgttgtgt tgtagaaata
    82381 aaagctcgcg cgagcagtcg ctgatacttc aaggtacttc aatagcggtt gcgcacaatg
    82441 atgacctgct ctgacggcta ccccttccat gtctaaagct gtcgcgacat catggggatg
    82501 taacccatcc aaattgaatg caacgactcc tgtccgaaaa ttcgggtctt gtggaccata
    82561 aacagtcaat ccatcaattg cttgtaattt tggcaacaca taagcggcta gttcttcttc
    82621 atatcgatgg atattgtcca tgccgagttc ttgcaaataa tcgattgcat ggcctaatgc
    82681 gatagcgccg gcgatatttg gtgtccctgc ttcgaatttc caaggtagtt ctttccatgt
    82741 actgtcgtat aattcgacaa aatcgatcat ttcgccacca aattcaacag gttccatttc
    82801 ttctaaccat tttcgtttac catataaagc accaatacct gttggtccgc acattttgtg
    82861 tccgctgaaa gcaaagaaat cacagtctag tgcttggaca tcaatggccc tgtgcggggc
    82921 agattgagca ccatctacta cgatgacccc gccgttttca tggacaagtt cagctaattc
    82981 ttgaacagga ttgatgacac ctaagacatt ggatacatga gcaatggaga cgattttggt
    83041 tttgtccgta atctttttcg ctgcatcctc catatccagg aagccttctt ctgttagttc
    83101 gatatatttc aagacagcac ctgttcgttt tgccaactgc tgccacggga taacattaga
    83161 atgatgttcc atgtaagaga gcactatctc gtctcctgct ttgatatagc ggtcgccgaa
    83221 gcttctggct acccaattca agcttgtcgt cgtacctcga gtaaacagca cttctgctgt
    83281 ttcttttgca tggatgaacg cccgtacttt ttctcttgct tgttcataat catgggtcgc
    83341 gcgttctgct aacgtatgga caccgcgatg aacattggcg ttatcgtgtt cgtaatagtg
    83401 cgctaatgcc tggagtactt gttttggctt ttgggtcgtc gctgcattat ccagataaac
    83461 taaaggttca tcattgacgt cttgaaataa gataggaaaa tcattgcgta gctttttagc
    83521 atcgatcatc cattcaactt cccttcgatt acttcgacta attctttccg aacttgttct
    83581 acagggattg ctgttaagac agaaccgagg aatcctcgaa tcactaagcg ctctgcttct
    83641 tctttatgca atcctcgact catcagatag tacatttctt ctggatcaac tcgtcctaca
    83701 ctggcagcat gtccagcagt tacttcgttt tcatcgatca aaagaatagg attcgcatca
    83761 ccacgtgctt tatcagaaag catcaagaca cgactttctt gttgggcatc tgcacctttt
    83821 gcccctttca agatgtggcc gataccatta aaggtcaatg ttcctttttc tcggatcact
    83881 ccgtgttgca aaatatgccc gatcgtatga ggggctttat tggttactcg cgtatcgatt
    83941 ccttggattt gtctgcccga gctgattgcc acgattttga cttctgcgtg tgcaccttct
    84001 cctgctaaat ctgagtcaaa gtcagcgatc acatgaccgt cattcatgac accaattgcc
    84061 cagtcgacag aagcatctcg taaaatgtgc ccacggcgat tcatataaga agtaatattt
    84121 tttcctaatt gatctacggt tgaatatttg atttttgaac cagctttagc gatgacttcc
    84181 acgatgatat tacctgatga ttttgctact tgttctttgg ttgtctggaa acgttccaga
    84241 taagaaaatt cactatgttc atctgccacg atcaacacat gcttgaagaa gtgctcatcg
    84301 gatgcgccat cttgaatgaa taaggactcg agtggttcat cgatcacgac atttttaggc
    84361 acatacagga aaataccgct gttcataaaa gctacatgga gtgcagtcaa ttggtcttcg
    84421 ttcatgttta ctgctttttt catataatat tcttggacta gttctggata ttcgatcatt
    84481 gcagtgaaca agtcggtaaa aatgactcct ttttctgcta attccacagg cagttgttca
    84541 aaaatcgtcc atgaaccttg ttggacaagc acaggattgt ctttcattgc atcaaatgcc
    84601 ggaatcgtgc ctgtttcact gatggtttgt gtttctttca catcaaataa cgaccagcga
    84661 tggaatttca cccgatcgat atgcggcaat ggcagttcat ctgctttcgc tagtgcgtct
    84721 tgacggaagg ttctcatcca atcaggttct cctttactta aagaaaaatc atttaccgcg
    84781 tcaagataat cgagccagtt tttttctttc attcttgctc ccccttacgc ttcttcttct
    84841 ttatagtcga ttcctaattc tttactgata ccagcgtagc cttctgcttc cagacgttta
    84901 gctaggtcag cattccctgt catgactact cggccttcca tcatgatgtg aacgacatca
    84961 ggtgtgatat agttcaataa tcgttgatag tgggtgatga tcaatgcacc gaaattgtct
    85021 ccgcgcattt catttactcc tttagcaacg actttcaaag catcgatatc cagacctgaa
    85081 tcgatttcgt ctaaaattgc aaatgttggt tctaacatca acaattgcag gatctcattc
    85141 cgtttctttt ctccgccaga aaaaccttcg ttcagataac gttcagccat ttcttctggc
    85201 atatttagta aagccatttt ttcatctaac tttttcaaaa atgccataac agaaattttg
    85261 tcgttttcat cacgtttcgc attgatcgct gcacgcatga attccgcatt cgtgatacct
    85321 ggtatctcac ttggatattg catagctaaa aataagccaa gtcgcgcacg ttcatctact
    85381 tccaagtcta aaacattttt cccgttaaac aaaatttcgc cttctgttac ttcatagttt
    85441 ggattgccca tgatagcggc agataaggta gattttcctg tgccgttagg tcccatgatg
    85501 gcatggattt ctccggtttt cattgttagg tttacgcctt taaggatttc tttttcctcg
    85561 atcgacacat gtagattttt tatttctaag acagacatgc attatacccc caaattctct
    85621 ttcgttctca ctcattttag actaattgag aatgaagcgc aatgaccttc cttactaaaa
    85681 tcaaatatct tttatttatt attaaacaat tttgatttct aaattagaat agatataaaa
    85741 aagatgagaa ataagccatt acagacttat ttctcatctt ttttgttcca taccttattt
    85801 tgcatcagcc tttactggtt taacagagcc gccccatttg tctgtgatcc attcttggac
    85861 ttctttgcgg tgaagcactt caatcagttt tttgattttg tcactgtctt catcaccttt
    85921 acgagtcgca ataatattca cgtaaggaga gttgtccttt tcaagcaata cggcatcttc
    85981 taatggattc aaccctgcgc cgtaagcgaa gtttgcgttg attgcaacta ggtcaccttc
    86041 atcattttgg tacgtagaag ctaaaagtga tgggtcgatc gtatggttga acttcaagtt
    86101 cttagggttt tcttcaatat tatcgaatgt tgctgtttcc agatctactc catctttgac
    86161 tttgactaag ccagcatctt gtagaattgt gatgatgcgt ccccagtctg attcagaatt
    86221 tgaagtaatg attgttgcac catcttttaa ttcggaaaca tctttgactt ttttagagta
    86281 caagcccatt ggttcgatgt ggacagcacc ggcattgaca aagtcatatc ctttttcttt
    86341 gatttctttg ttcaaataag gaatatgctg gaagtaattg gcatcaatgt cgccttcttc
    86401 caaagattgg ttcggtaata catagtcatc gaatttgacg atttcaagat cgattccttc
    86461 atctttcaat aatggtttca catgttccaa aatctctgcg tgcggtgtcg gtgaagcgcc
    86521 gactttcaat gtcgtgtcgt ctgctttatt tgctgaatct ttgctgtcgc tgttgttacc
    86581 gcatgctgct aatcctacag tcagcaaaag cgctgctgct aatccaaaaa ttttcttttt
    86641 catatcctac atccccctat atcctattat cttttgtctg ttttcttagt taagaaatca
    86701 ccgattcctt gaacgataaa tacgattagt aaaataatta aagtcgcaac gaatgtcact
    86761 gtcagattac cacgctggta gccttcctgc caagcaagac ctccaagtcc acctgcgccg
    86821 atcgcgccgg ccatcgcagt aaatccgatc atcgagatcg ttgtaactgt taatccagaa
    86881 atcaatgcgg gaatactttc aggtatcaag accttccaga tgatttccca gtaacttgct
    86941 cccattgcat ctgctgcttc cagtacacca gggctgactt cacgaaaagc aatctctact
    87001 agacgggcat aaaatggtgc tgcggataaa acaagagctg gaatggctgc ttgcgcacct
    87061 agcatagtgc ctacgattgc tttagtaaat ggaatgatca aaaccatcag gatcataaat
    87121 ggcgttgacc ggaaaatatt actgatgatc gatacgattc cataaatgat tcgagcatac
    87181 ggtttgtttt tgcgtccaat agagtaaagg atcaatccca aaactaatcc gatgatcgtc
    87241 actaagacca ttgagattaa tgtcatatat agtgtatcaa ggatagcctg attcatcgtt
    87301 tccgggttga tttgtttaaa atctagatat gtttctgaaa agcttttatc cacgatggat
    87361 cacctcaatt cctactcctt gttctttgat gaacacttct gcttgatcta attctttggt
    87421 ttcaccagtc atcaagacag tcaatgaacc aaaggaacct tctttcactt gtttgatctt
    87481 tccatacaca acgttgacat cgatgttata gcgtcgaatc gcttgggaaa tgactggctc
    87541 gttggcattt ctttcgctaa aggtcaaagt tgccaaacgg ccgcttggat tttctttgat
    87601 gagttcagct aatagttctg tcgaatcagc ttctggttcg atgtcttgtt gtacaaagcg
    87661 tttggttact tcttttttcg gttgtctgaa gacagtcaat acgtctcctt cttctacgac
    87721 tttccccaac tccatcacag cgactttgtt gcagattttg cggatcacat tcatttcatg
    87781 tgtgatcaag acgatggtca aattcaattt cttattgatc gctaacaata agtctaatac
    87841 ttcttctgtt gtttgcgggt ccaatgcgct agtagcctca tcacaaagca ggatctcagg
    87901 atcatttgcc aatgctcgtg cgataccgac gcgttgtttc tgcccgccgg aaagttgtga
    87961 aggatatgct tgtccgcgtc cttctaaccc gactaaacgc aacagttctt ctgcccgttc
    88021 tttacgtttg ctctttggta cgccagctaa ttccagcggc agctggatat tttcaagtac
    88081 agttcttgac cataacaggt tgaaatgctg gaagatcata ccgattttct ttcggaatgt
    88141 acgtaattct ttgtttttta attttgtaat atcttgatcg tttacgatca cttcgccgct
    88201 cgtgggaagc tcaagtccat tcaggagacg gactaatgta ctttttcctg cgccagaata
    88261 gccgacgatt ccataaatat cccctttttc aacgtttagt gaaacgtcat tgacggcttg
    88321 gatgtttccg tttttcccat tgaatgtctt tttgacattc gttagttgga ttaatgacat
    88381 gccattcaca tctttctctt atgatttttc aaacaaaaaa gccttcgccc tcttgcctta
    88441 agcaaaagga cgaaagcttg aacttccgtg ttaccacctt gatttaccgt tacttcgcaa
    88501 taacagcctc ttccagtact tggacttagc ttctttccgt atactgtcgc gctgtaacgg
    88561 gcactcccgt agtaagtttg cttacgctcg cttactctgc tctaagacca tcttccatct
    88621 cattttcgtt acccattttc agcaaacagg gctctcttca aacgtccgtg acatgtactc
    88681 ttcttttcaa agctttctcg tttcatatag gattgcttat gattatagca actggaagaa
    88741 tcagtgtcaa cttttttcaa gtgattttct gattttataa ttcgtcaaat tcttttggat
    88801 caacatcttt gaaacttaag atccacgcat tcatttcatc tgaatcattc aagatattga
    88861 tatcttcacc gattttatca ttaacagaag aaactgtacc cgttagcggg ctggcaaatt
    88921 cattgaccgc tttttccgct tcaacttcga tcagaggttc atcttttttt agcgattgtc
    88981 ctgcttttgg cagattggca aacgtgatgt ctccaagttc ttcttgcgct tcatttgtca
    89041 aaccaacaca gtattctttt ccattaaata agatccaaag gttgtccttt ttcttcaaat
    89101 gcttgcctgc cattttatat cagttctcct tcataaattt tttcattgaa tccaattgca
    89161 acaagctggt cctctcgtaa gatcagtggc cgcttgatca gcataccatc tgtcgataag
    89221 acttggctcg cttttgaagc cgtaaactcg cctacttgat ctttcagtcc aagttcccga
    89281 tatttcatcc cgcttgtgtt gaacaggcga cggattggta cttgactcgt ttctagccaa
    89341 gtctcaatcg ttgaggcctt cggtacttct ttgatcatat caacagtttg ataactaacg
    89401 ccatgctggt ctaaccacgc tttggctttt ttgcatgttg aacatttcgg gtaccagtaa
    89461 aaggtgtaca attttcttac ctccttgaag atcttgcagg tgcttcattt tggtatctca
    89521 tcgataatat caacccgata ccgatcatgt tccctaaaat cgaagatcct ccctgactaa
    89581 tgaaaggcaa tgggatccct gttaaaggaa gtaatccgat atttgctcca atattctcaa
    89641 agacatggaa cagcagcatc atgatcaaac cgcttgcgat ataggcgtaa aattcattat
    89701 tcgtatcgaa acacacccga atcatacgat agattaaaat aaaatacaag aaaatgacaa
    89761 atgcaccacc gataaagccg aagttttcac cgacaacggt aaaaatcata tcggactcac
    89821 gaacaggcac ataaatattg cttacgttga atcctttacc aaacaagcca ccggtaccaa
    89881 ttgccaaaag tccttgggcc ggctgataac tcattcctga cgtatcatgg aaagggtcca
    89941 gccatgaatc aatacgtgca aactgataag atttgaagcc aactttgtaa agcaagtctc
    90001 ttccgccttc agtcgtaacc aagaagatcg ttcctgctcc aacgactact gccaagatta
    90061 ctacaggtgc gatgatgcgc caagagattc cagacatcaa gaaaatcccg ccaaagatcg
    90121 caagaaagac aagcatcgtt ccaaagtcat cttgggccat gatcaatgct aaaaccggta
    90181 ttgtcacgat caagagcttc ccaatcagcc aaaagtccga tttcatcgtc cgttctttta
    90241 cttgcgtatt atgtttagtt acgatcagtg ccatcatcaa gatgtacgca atcttcatca
    90301 actctgctgg ctggaaagta aacgcaccga atcgaaacca gtttttagac cctgttgaag
    90361 cagctaaatt ctgatcatag aatttgagca agagtcccat taccactaac cccaaaccat
    90421 aaaagtaagg agtcagtttc cagatccatt tggaattcaa atgcatgatg acaacgattg
    90481 ctgccgctcc aactaaatac cagacaactt gcatccctac gcctttcaag actgctggat
    90541 tgttcggatc atggctaagt gctacatata atgagagcag accaatcaaa cataacagaa
    90601 agactggtaa aatcacacca taatcaatac gattatcgtt cgaagctttt aatttttttt
    90661 ccatgtttca tcccttttct taaaaaccat actgctgatt attatagcgg tccagttaga
    90721 taatggaaag tctttccata gacatttggg aaaaattaac tttccgtttg tgcagttgct
    90781 tgagcaaatg cagtacaaaa gcctactgct tatctttagt gtatcataga actttttttg
    90841 atacatgcaa ttatctacca actgagattc tcctcgctgt gagaagtttc tgtgctttca
    90901 ttgtatcgaa ggatctcttc ggcaatctct agcagctgat cagaaaccag attggttcgt
    90961 tggatgaaat aaaaataacg atggaaattc aacggagtca ttggcacatt ttctggaaca
    91021 gagcgcttag acaaaatcga gcgaccgatt ccttccttca gcaacgcaac aattacatcg
    91081 ttacttttca cgatcatctt cttatcttgg atattcatct cttcaaaata acgctgcgtg
    91141 taatgaaaaa caccagagtt tgcttctctt acaagccaca atttgctttt tgggtcccca
    91201 gcgatgacta gctggtcttc catcaaagag tatctttgta cgccggaagt ctctaatggc
    91261 ttttcgataa aaccaaaatc ggattcatgt ttcgctactt gttctaatga ttcatgagaa
    91321 ttcgcgattt cgatctcaaa atgcacttta ggaaaccttt tgatcagatg tttcatcaac
    91381 tgaggcaaag gatagacagc aaaggtgtgg gaagcaacga ttcgacatac ttcttcttca
    91441 ttttgttcat tgagcaattt ttgctgagtt tcttcccatt cttctaacaa aaccagtgtc
    91501 cgttcatata ataagtcggc ttgcttagtt ggaagcatct cttttcttcc gttacgttga
    91561 aaaagatcga tattcaattc ccgctccaac tgtttgattt gattcgagac tgctggttga
    91621 gataaaaaca gtacttctgc tgctctggaa aaatttcttg tttcatatac tgctcgaaaa
    91681 gtcgttaata acttaaacat ggcaccactt ccataacttt ttatgataat tactataaaa
    91741 actatttgat ttaaatatac aaaaaaccat ctataataga aatgtacaac aaaacagttg
    91801 ctgatccaac tagtaactta gatgaagaca atcagctgtc tgcttgctcg taatcataca
    91861 ttcctattca ttttacaatg tattcgttca tcttggtact aaaatctatt aaataaaaag
    91921 gagaatatta tatatgaaga actttttgaa aattattccg atcccgatct gcgggttgat
    91981 ccttgggtta gcttcccttg gaaatctgtt taaagactat caccatgttg ctttaggcaa
    92041 tctaactggc ggtatctcta tgattcttat gatattcatt tttgttaaat tgtttatctt
    92101 attcgaacac acgaaacaaa cgttgcatga tccaatcatc gcctccgttt caccaacttt
    92161 tacgatgtca ctcatggtta tttgtactta ttttgtttca tatgaaagta ttgcaccaat
    92221 tgtcaaattc atttggctgt gcgctgttat cttccaagtg atccttgtct tttatttcaa
    92281 ttatcaccat gttatcaaag ctgatctttc aattgaagca atttatccaa gctggtttat
    92341 catttatgtt ggatttgggg tcatcactgt aactgccggg aacttcttcc cgcttattgg
    92401 aaaaattttc ttttggattt cattgatctg ctatgcgttc ttattgccga tcatcatcaa
    92461 acgtgtcttt ttcgtaagaa atatggctca tccgacacgt ccgctgatta cgatcgttgc
    92521 agcacctggt tctctttgcc taacaggtta tcttaaaaac tttgctgaac ctaattttta
    92581 tcttgtcgta gcattgttcg ctttatctca agtattgtat ttcgttgtgc tgtctatgct
    92641 tccacgtttg ttcaagcttc gtttttatcc aagctatgca gcctttacat tcccgcttgt
    92701 tatctctgca acagctatat ttactactgt tcattacttc aacagtttag gtatctatag
    92761 tagcgtattg gatttcttaa aagttgcgga atgtctgttt gcaagtgtag ttgtcttcta
    92821 cgttcttgct cattacgtac gtttcttaat taaagaacat agcaaacatg gaaaatttgt
    92881 ttctaaaaaa atcgcttaat aatcgattca ataggttgtg aaaaagctgt tctgcatcaa
    92941 gtaataagaa cggccaaaca aaaacagctg ttcatgtttt tgaattggtt gggcttatta
    93001 ccgcagatgc aagcttttga acaccgttta ccaagaggtc gtaaaagaag tggccagctt
    93061 caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa tttcggctta
    93121 tttccgaaaa agctacttct ggaacaccgt ttaccaagag gtcgtgaaaa agccgcttag
    93181 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc
    93241 ttaataccga gaaactggct tttgaacacc gtttattcga aacaagaggt cgtgacaaga
    93301 cttttgtcac gacctcttgt tcttttgtca aaatactggt ttagtgctac tatgttcgag
    93361 tatgctttta gtatttattg acgaaaggaa ccgatcaaat gaaatcattg tcacatccaa
    93421 taaaatctgt aaaggattca gcggtccgcg aaatcttacc cggtttactg ctttcttttg
    93481 ccatcgctgc cttcagcagg cttcttgcta tttatctgcc acagttaggt ggagcgacta
    93541 ttgctatttt attgggtatt ttattaggaa atactttttt taaacagcct gttctagcaa
    93601 tcggcacaaa atttgctgaa agcaaactat tagaattctc tgttttttta ctaggtgcaa
    93661 ctgtcacagc tcaaacgatt gctcaaattg gtataaaagg aattgttttt gtgttactgc
    93721 aaatgtcagt cactatcatc ggtgcttact tgattggtaa aaaactgcaa ttcagcgata
    93781 cttcttcttt gatgatggct ggaggaaacg ctgtttgtgg ttcttctgct attgcttcga
    93841 tcgccccttc catccatgcg aaagaggaag aaaaaggaca gatcatcaca ctagtcaatc
    93901 tactgggtac tgtgatgatg ttgactctcc ctttccttgg attacagttg tttggtggaa
    93961 atctcattga aaaaagtgca cttttgggtg gcacactcca atctgttggt caagtagtcg
    94021 ctggggcaag cttacttgac aattctgttg tcgaattcgc catgctattt aagattctac
    94081 gtattatgat gctcgttgtt gttgtctatc tgtttgaaaa attcatgcac aagaaacaac
    94141 cagctctgtt tgaaacgcca actgatacgc agaaaaaacg aaaattacca cttccttggt
    94201 atgtattagg gtttgtgatc ttttgtttgg tcaacagctt gttttcttta cctgtagtta
    94261 ttgaccaatc ggcacatttt atcagcagct ggtttgagat cactgcttta gcggcaattg
    94321 gtctgcgttt agactttgca aaattcatga aagaaggtac acgcttctta tcttatggat
    94381 tagctgtcgg gcttctccaa acagtgatgg ctttactatt tatttggctg atcaaagttt
    94441 agaatgaaca tgaaaaaaca gttgtgattc tcgcatggaa caatgtccga atcacaactg
    94501 tttttaattt tgcagatatc cttctaacaa caacaattgg cgtttgattt caaccccatc
    94561 tattgcgttt cccatatatc ctgtcagttt tccattttgc ccgatcactc gatgacaagg
    94621 aataacgatt ggcaatggat tggttcgatt tgcctgccca atcgctcgca cagctttcgg
    94681 actatcaaca gcaacagcta cttctttata cgttcggatt tctccataag gaatggtcga
    94741 caaagctttc cagacatttt gctgaaaagg cgtacctctc tttatagata aaggaacatc
    94801 aaattctttt ctttttcccg caaaatattc ttcaaactgt tcttgtgtgc gcgttaaaat
    94861 ttgctgattc gctgattcgc tttctgaaat tggctgaaaa gatacagcag tcaatccgga
    94921 ttcatctgca tctaaccata aaaagccgat aggtgtgtgt attttcattt tgtttgctcc
    94981 tggtcagtag actggaactt cctccttttt ctctatccaa aactacttgc caactaataa
    95041 aaattgttct taaaagtttg ataaaccgag gaaacatggt aaactaaata cgttagttta
    95101 ccaaagaata gctgttcttt tccataaaat caaatttaaa aggtgttatt taaggtttat
    95161 taaattttga atgtaaacca tatataaaca tacgattgaa aggatagaaa cgatgaaagt
    95221 atcaattgtt gtcccatgtt ttaatgaaga aaaaactgtt cctctttttt ttgatgaggt
    95281 tgaaaaattc aaaggtgccc atgagttcga atatttgttt attgacgacg gatcaaaaga
    95341 cggtacgtta gcagcaatca agaagctagc ctcagcttat ccttatgtcc gctttgtctc
    95401 attttccaga aattttggga aagaagcggc tctgtacgct ggattgcaag cttctacagg
    95461 agatttagtg accgtgatgg atgttgatct gcaagatccg cccgaactgc ttcctgagat
    95521 gatcaaccgg atcactactt ctgatgtcga tagtgtcggc acccggcgtt cgactcgtga
    95581 aggcgagcct gcgatacgca gtttctttgc gaaaaaattc taccagttga tcaataaaat
    95641 cagcgataca gaaatggtcg atggcgctcg tgatttccgc ttgatgactc gtcaaatggt
    95701 ggatgccgtc ctagaattaa cagaatacaa ccgattttca aaaggattat ttagctgggt
    95761 cggatttaaa acagaatata tttcttatga aaaccgcgaa cgtgtggctg gcgaaacatc
    95821 ttggtccttc tggaaattat tcaactactc gattgatggt atcgtgaact tctcggatgc
    95881 accgttaacg attgcttcat ttatcggcgc tttgtcttgt gtcggttctg gtattgcaat
    95941 tttgttcatt attttgcggg cattgttgtt tggtgatcct acatcaggat ggccttccat
    96001 ggtctcgatc ttcctgttca ttggtggaat ccaattgtta tgtcttggaa tcatcgggaa
    96061 atatatcggg aaaatcttct tagaaacgaa acagcgtcct gtttatatca taagagaaac
    96121 agaaaaagtg aagccaaatg aattgaagcc tcaccaataa tatacgtcaa atgaagtaaa
    96181 cattttttta aatgaaaaag gactgtgaca aaagtcttgt cacagtcctt ttttccgaat
    96241 aaatggtgtt ccagaagtag cttcttcgga aataagccga aatttcacaa aaatttgaga
    96301 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctatc acaacctctt
    96361 tcatatctta ttacttcttc attgctgctt ttagtgctgc tgctaatggg ctttcttctg
    96421 gttcttctgc ttgtgcatat tttttcagca agcgtcgttc ttcatgtttg gtcattttct
    96481 tcttctgctc ttttttatca agcatttttt ctgtcgttcc atcgtattta caacggaaat
    96541 aagcaccgtt tttaccttcg atgatttcca ttttccggtg acattgcggg catcgatgat
    96601 tagagacttt tggatctttt cttctgcggt agttgcaatc tgaattggtg cagacataga
    96661 ttttgccgtc acgtgtgttt ttctcgcgca gattggcccc acattctggg catttctttt
    96721 gggtaatcga gaaatcttga tattcttgtt tgcttgattt gatctcattc accaagcgtt
    96781 ttgtttcttt ttcgattcca ttcaagaagg ttcctgcttg tttctttcct gcagcaatag
    96841 cttcaaggtc tttttcccag ctttctgtca aatctggtgt tactaaagag ggatttacca
    96901 atactaataa ttgcttacct ttaggagata cttgcagtcc ttgaggggta cgttccatca
    96961 gttcagattt gattagtttc tcgataatct ctgcacgcgt agctggggtt cccagactgt
    97021 atttttccat ttgaccaagc aagcttgctt ctgacaatgg tttaggcggt gcagatagtt
    97081 cttttttgat ggtgaaatcg ggattgattc tcatttcttt ttcccatttg actgtttccg
    97141 tctcagtttc agtttcgcct ttccatcctg cttggacgac acgattttgc cgaaaaatga
    97201 atcgttcttt accaaattca accgtaactt ttgtttggct gactttatgg ggttgagcaa
    97261 acagtcctaa aaaacgagag acaatcattt gatagatttt ttgctcttca ttacttaatt
    97321 tttcataacg tgctctgttt tcagttggca aaagtgcatg gtgatcggtc actttactgt
    97381 cttggaatac ttttgtctgt tggacgatgg ctccgttttt caaatactgc ttgacctcag
    97441 gagcaaagtc gctgactgct tgcaatcgtt ctttcatcgt acttttcatg tcatttgtca
    97501 gatatttgct gtctgtacgc ggataagtaa cgatcttatg ggtctcatac aaactttgta
    97561 caagacttaa tgttttcttt gcagaatagc catagcgttg attggcttct ctttggatct
    97621 ctgttaaatc ataaggcaat ggcgcagatt ccgtttttaa tttttcttgg acttctgtga
    97681 cagttccttt gcttgtactg atcaattgaa caagtttttg tgcttcttct tgtgttttta
    97741 aagcaaattg atttttttga agcattttgg cttgttcttt tccagcttgc aacgtgatcg
    97801 tgaaatacgt ttgtggacgg aactgctcga ttttcttttc ttggtctctc actaatgcta
    97861 gtgtcggtgt ctgcacacgg cctgctgata aattatccgc gtacttcaca gttaaggctc
    97921 tggtcacatt caatcctacc agccagtccg cttttgcccg ggcaagagca gattcataca
    97981 atgcatcata ttctttcgca ggtcttagtt gcttgaatcc agttttgatt gctttatctg
    98041 tttgagaaga gatccataac cgcttcactg gtttattgaa atgcacatac tctaagatcc
    98101 agcgagcaac aagttcccct tctcttccgg catctgtcgc aatgactgct tctgagacat
    98161 cttttcgctg agccagctgc ttgattgctt ttaattgatg tcctgtttta ggaagtggtt
    98221 tgattcctag ttttttaggg atcattggca acgtgtccat ttgccagctc tgccattcct
    98281 tattcagatc ttccggcatt ttcaatccta ataaatgacc aagggcccaa gtaacgatca
    98341 ccttgggtcc ttcataataa tttttgtgct tttgattagc accaagcacc ttgcttaggt
    98401 ctttcgcaac gcttggtttt tccgctagaa ttagttgttt cataaaattc ctttccgctt
    98461 tctactgtga tgggctgatg atttttcaac aacgcaagtc ctttgtattg ctgtaaatca
    98521 tcgtcacact cttcacacca gcgctcatct ccgtcattcc aaaaggcgct gagaaagtta
    98581 ctttttgcat tgatatattc ataattttga tggatttcgt tccattgttt ttcgtacgct
    98641 tgtcgtgctt cttcgaaaga ctcgaatgtt tcttctgctt caatatcttc ttcccagtct
    98701 tcaaaaaacc accaaggctc attgtccccg aacatggtaa ttacttgata catactcgtg
    98761 ctccttcttt cagcttttct agacttatct tagtcacttt tcaagaaagt tgcaagaatt
    98821 ttctcttata aaaggatcat ttaatgaaca tcttgaaaat gatataaatg aatctgattg
    98881 tttaaggcaa agtagcgata aagtcattga tcatccaaaa atagctgtcc tctggatcga
    98941 ttgtcgattc ttttgtaaag tatcctaaaa aacgcaagga aagatagccg tggatcatac
    99001 tacataattc tctgcttcgg tggacttttt cttctttcgt taattgaaaa gcctcaaaga
    99061 tctgcaaaat gatctggttc gtttcatgga ttccctctaa taaaactgct tcattggtgt
    99121 ggggcacact gatcagtaac tcatacactg cttgatttga aaaagcaaag gatcgataag
    99181 tttctgcata gatctgcagt gcttcctgac cactttttcc cacaagagcc cgacgcagct
    99241 ggtcattaag ttcctgcaga agtttcgctg ttagcgctgt tttgacatca cgaatatttt
    99301 tgaaatggtt atacagcgag ggagccttga tatccaagcg ttttgctaaa tgctgaaagg
    99361 ttatttgctg aacatccatc tcttctgcta gttctcgaaa gcagtcgatg attttctctt
    99421 gagacagatt tctttttttc attttcatgt ttcctctcat aaaataaatc cttcactctt
    99481 tcagtttacc acgaaaaaga aagagaaaaa agagactttc attgataaac tacatattgt
    99541 agtttataac aaaaagctgt agatgaaaat tttcagaaag aaggagtcta attggctgca
    99601 agaataaagg aaaaattatt taggaaaaga gaaacttggg aacaaaactt gtttgtctta
    99661 tggtttggaa catttatggc tggtatcgga tttagtttag tcatgccttt tatgtcactt
    99721 tacatagata cgttaggtaa ttatagtacg tcacaattga acttttggag cggattgact
    99781 ttttcatcta ctttcttggt gactacgctg atctctcctt ggtggggaaa attagcccac
    99841 caaaaaggaa gaaaattgat gctgttgcgt gcttcattag gaatggcaat cgtcatttct
    99901 ttgatgggtt gcgtgactag tgtgtatcaa ttagtcgcat tgcgtctgct tcaagggatt
    99961 ttttctggtt atatcagtaa tgccgctgcc cttgtcgcaa ctgggacgcc aaaagaaaaa
   100021 agtggtcaag ttcttggaac tttagcaact ggttctgtta caggaacatt acttggaccg
   100081 cttcttggcg ggatctctgc ttcagcgttt ggctatcgcc ctaccttttt cattacaggg
   100141 atcatcttat ttttcgtctt cttgttgagt ctgtttttcg tccatgaaaa atttgtccca
   100201 gtgaaaaaag aaaacatggt ttctgccaaa cagattttca aagacttgaa atatccacac
   100261 gtcgtcatcg gtatgttcgt cacaacgatg atcatccaag catccaacaa ttccattagt
   100321 ccaatcatca gtctgtacat tcgccaattg ctgcatggac atgggaatgt tacgttgatc
   100381 agcggaatca tcgcttcgat acctgggatt gccactttga tagccgctcc aagatttggt
   100441 cgcttaggtg acaagattgg gagtgaaaga atacttgcga ttggtttggg ttttgcgatt
   100501 ctggtttata tcccaatggc attcgtcacg aatgtttggc aattagctgc cttacgtttt
   100561 ttgatgggta tctctgatgc ttgtctgctc cctgctgtcc aagcattgat cacaaaatat
   100621 tctcctcatc aagctgctgg acggatcttc agttataacc aatctttcca agcaactggt
   100681 aacgtagtag gacctatgat cggctccagt gtttctagtg catttggtta tcgtggcgtc
   100741 tttctttcga cttctcttct tgttttggtc aacttcttgc ttgtacgaca caatacacaa
   100801 gagatccatc agcaagaaac tgcagaagaa aaagcttcag gagtctccat atcttcaggg
   100861 cactctcctt ctcatagaca ataagcgtaa atgaaaaaca ctgcttgaac agttgtcaca
   100921 gactgtcctt gcagtgtttt tgtttaggcc tgttttttca agaactcaga taagaggaat
   100981 aatccctttt ccaaggtttc ttttttacaa cagtaaccta gtcgggcatg cttcggtaaa
   101041 tcaaaggcac ttcctggtac taatagtact cctgtttctt ctaataaatt gatacaaaat
   101101 gtctggtcat cttcttcgat ggctaattta atgaaagaag tagagacgta attgggagcg
   101161 acaagttcaa ctttcggttc attggctacc cattgtttta aaatcacaag gttctctgta
   101221 atgatctttt tgtttcgctc caatattttt tctttattct tcaatgcatg tacagcaaga
   101281 tcatctgaca ggactccgcc gcaaatcatc gtgtagtctc gatactttcg aaaaacttct
   101341 gccaactcag gtccagtagc tgtccagcca atacgaatac ctggaatgga gtaggttttg
   101401 gatagggaat tggttgctat acctttttca tagacatcta cgatcgacaa gaattcacct
   101461 ttatccgtta aaggcgcata tacttcatct atcaatacat atgcatctac tgtacgagca
   101521 atttctgcga ttttttctaa agttttccga tctagcaatg tgcctgtagg attgtttgca
   101581 ctattcaaac agatgatttt tgtttcaggt ttgactaagg cttctagctg ttctaaatca
   101641 aactgccaat cttcttcttc cttcaaatga acaaagtcaa cagcggcacc taaagatttg
   101701 gggatgtcat ataattgctg ataagtagga agcatcgaaa caacatggtc tcctgcttcc
   101761 actaacgcgt atagagctaa aagattagca ccagtcgctc cattcgtttg taagatgttt
   101821 tccggatcga cggtttgata aagagaagcg acttcttgtt tgaaaacatc tgatccttcg
   101881 atccagccat agtccaatgg ttcgtttttg cgtttttcga aaaattcact tacgctagtc
   101941 ccatctaagc caacgagttc gtctaatgtc aatgcttcga tcgagctttg tgcaatatca
   102001 tataccgctt tcttttccca tttatttagc cattcttcta caccgaaatt tgcgattttc
   102061 atgtttcttt cccccttata tcttgatcca gcctacagca agcaacccga ttgaaagcag
   102121 tgcggccaca caaatcagcc caatcattac tttttctttt cccgtaattt gatgtttggt
   102181 ttcacgacaa gcgatccagt agaagcaaaa accaggaaga taggcaatac tacataaaag
   102241 gacataagaa aatcctgcta aaatgatgcc catcaattca aaagctacag caatcccacc
   102301 gatcactact tgttttttga ttccttcttt taaagaaaac tggacttgat agatcccgac
   102361 aagtagataa caaatcaaaa ttgccgctgt acataaagaa taggcaaatt gataggcata
   102421 atcagtaact aagaaagtca ataagaatag attagtcaag attgcagtaa caactaacga
   102481 ataagttggg gcattttttt tattcgtttt tccccaacct ttcggcaaca gaccgtctcg
   102541 agccatcaac atcgttgttt ctgctgggag catcgtccaa gacaaccaag aaccaatgat
   102601 cgaaataatc aagccgatat tgatcagcgc tgctccccaa tttcccacga ttccttttaa
   102661 aatatttgcc atagcaggtt gtgaaatagt tgctaactct tcttgagata agacgccgta
   102721 tggcaagatc gaagcaagga tatagataaa caataaacaa agcagaccaa tgatcgatgc
   102781 ttgctgggct tctgaacgtt tcttcgcgcg gttggctaaa acacttgcgc cttcgatccc
   102841 tacaaagacc cacatcataa ccatcaaaca acctttaatc tgcaaccaga tcgttccttg
   102901 ctgccctgtt ccattcacta gattatcaga tacgttaccc caaaaatcag cagtgaatat
   102961 attcacttta aatgcgataa atacaaatat taaaaatagg aataatggca ctaatttaca
   103021 gatcgtcacg attgtattga tgacgctcgc tccttcaact ccattgttta ctaataaagt
   103081 cagaccccaa ataaaaatac ttgcaaataa aatagaagga acattttgtc cacctttgaa
   103141 tatcgcgaaa aattctccca tggcgctcat cagcatcgtt gcaaaagcaa catttcccag
   103201 ccacgcagac aaccagtaac cccaaccact gatgaagcca cctaatttac caaatgaagc
   103261 ttccgcgtaa ccgaaaatac caccatctag atctggacgt ttttctccta aatgattcag
   103321 cgataatacc aaagctaaaa taccaatccc aacgattatc catgaaagga tcgctggccc
   103381 tggagctgca gaactagcca gatcacttgt gatcccaaac acaccgcttc cgattgacga
   103441 actgacaacc agagcaacca gtccccattt gctaataccc gttgatttct tttccatttc
   103501 catcaccctc acttataaaa ttgaacaaat cacttgcaaa agatttcctt ctattttgca
   103561 actgctcttg cttaacaaaa agcaacttta ttcatattat tggatattat gaacttttta
   103621 cacaagtatt tcaagtataa aatgaataaa aattaatttt attgcaaaaa aacagctatg
   103681 ctatccaaaa gaaaaggtgt ttcttttgtt agccatagct gttttgattc atttaatatc
   103741 gcttaaacgt attgtaaaac ttttgtttct tgatatgcgt ggtcgatcaa accaccaccc
   103801 agacattcca tttcatcata aaatacaact gcttgtccag gagtgattgc acgtacaggt
   103861 tcgtcgaata cgacttctgc atgattatca tccaataagc gaactgtcac aggaacgtct
   103921 tgctgacgat aacggaattt cgctgtacac ttgaattctt ttggcattgg ttcattcgtc
   103981 gtaaagtgga tttcacttgc atccaagctt gtggcataca atgcaggatg atggaaccct
   104041 tgccccacat ataatgtgtt tgtcgctaga tcttttccta caacaaacca gggttcttgc
   104101 gaatctcctc cgccaccgat ccctagtcct tgacgttgac caatcgtata atacatcaag
   104161 ccagcatgtt gccctttgac ttcaccatca agagtgacca tatttccttt ttttgctggc
   104221 agatagttgc tcaaaaattg tttgaagttc ttttcaccaa taaaacagat tcccgttgaa
   104281 tcttttttct tagcagtcgc aaggcctgcg cgttcagcaa ttgcccgtac ttctgatttt
   104341 tccatgccgc ctagagggaa catcgttttt gaaagctgtt cttgtgataa ttggctaagg
   104401 aagtaagttt gatctttatt attgtctact ccacgaagca tgtggaccac gccgttttca
   104461 tcgcgagtga cttgcgcata atgtccagta gctacataat cggcgccaag atccattgca
   104521 taatccaaga atgctttgaa tttgatttct ttgttgcaca tcacgtctgg gttaggtgta
   104581 cgtcctgcgc ggtattcggc taggaaatat tcaaatacac gatcccaata ttctttttca
   104641 aaattcacag aataataagg aatcccgatt tgagcagcta ctttcgctac atccttataa
   104701 tcttccgtgg ccgtgcatac accgttttcg tccgtgtcat cccaattttt catgaatatt
   104761 ccgatcacgt cgtatccttg ttcctttaac aaaagtgctg ttacagatga gtcgacaccg
   104821 ccgctcatcc ctacgacaac gcgtgttttg ctgttgtctg tcatgatatc accatcattt
   104881 caattagatt ctgaaacatc tacgagttga aggtcgcaac ttttcagtat tttacattat
   104941 acgtcatatt tctgaaaatt tcatccctta attatcaagt cgtaaaaata ctgttctact
   105001 ggacctattc agtacacaaa catgatagtc aaccaaaaaa tcactcatct tttgagcatt
   105061 cgatataaaa aattggcata ctcataaaaa gggccagcct ttcggcaata aggaataatt
   105121 ttataaattg tgaaaaacta tttttcaaaa ttgcttctta tcgcttaaag ctacacgtct
   105181 catttacaac cttttctttt tagatgttat gagttgctca gataagtgat tttgtttact
   105241 ctaagaaaaa agggaggcaa aagatacatg aattacttta ccagtgatat gcactttttc
   105301 catgaacgct tattaggaaa aaacgacttt gctccgcggc tctttgcaaa tgtggatgaa
   105361 atgaaccaac agttgatcac tagctggaat gcagtaataa aagaaaccga ccatgtttat
   105421 catctaggcg accttgctat gcatccgcaa tatgaaaaag caaatgcaga aattttggaa
   105481 ctggtcactc aactgcaagg aacaatccac ttgatcaaag ggaaccatga cagtcgcgct
   105541 tttttccgtt atttagaaaa aaatgatcct aggcttactg acggaagtcc caaattttat
   105601 ttttatgatg taggtgccat cgttaaattc gaccatcaac aatattactt gactcattat
   105661 ccgatgttgc tcgggcctaa cgacactatc cgcaatcttc atggacatat ccatcattac
   105721 agcgtaccta tcgagaacga tgtcaatgta ggcgtagacg caccggaaag ggaactctta
   105781 aaggaaaaac taccatttgg cactccgtta tctgagcaac aaatcaaaga aatctatgaa
   105841 gcaaagagga agtacgatag gtcgtgaaaa aactgcccta tatcaagtat taaacaaata
   105901 aagataatta ctcatatttt tcatttgagt aggcttattg ccacaggaac attaggatca
   105961 gaaatggagt tgtaacaaga cttctgtcac aactccattc catactattc gtctttttac
   106021 tcatggtgtt cttttaccaa ttttttctgt aattctttat cataatttcg agcagctgac
   106081 gttaaaaata aaacgattcc gcataataaa atcccgattc cagaaaacaa gaacattttt
   106141 tctaccccta aacgatcagc taatggtcct gcaaataata atccaattgg acctgccagg
   106201 ttcatcaaag agttgaagtt ccctaagaca cgacctaaaa cattcgattc ataactctgc
   106261 tggatcattg ccatcagcag tgtattgaaa tatggcgttg cacaacctgc tcctgcattc
   106321 aagataagaa agtacaagaa cccttgacta tcgtttggac ccaatccgct tgcaccgatc
   106381 gtcaggccca taatgatgta ggctataata ataggtttca ttcggtcttt ccatgttcct
   106441 ataaaactga tcagtgcacc gcctaagagc attccgactg cgtagactac ttccactgct
   106501 cccgcctgtc caacggaacc accaaagtaa tctagcgtca tcaatggata cagactgatt
   106561 gctggcatga acaacagcat cacaaatgca ccattgatcg ttatgtacca taaccctttg
   106621 ttatccatca attgctgcag cccaaatttt gcatctttaa aaatagtaag cggaacttcc
   106681 aatatttcag gagaaacttt tgggatcttc acaaaaatca ataagccgac accaaaaact
   106741 gcccccaata catctaataa aataaggtaa ttgacaggaa cgactgaaaa caaagccgca
   106801 cctagagctg gcgcaatgat gaaattcgct gattggacca ttcctaactg tccattcgtc
   106861 cttgttatgt gagaagatgg aacgattgtc ggcataatcg actgatcgtt ggcatttgga
   106921 atgtttgtgc gaccgaacgc ataaacaaag acacaaaaac tagccaaatc gggaaggatt
   106981 ctgaaatcgt tccgactact gctaaaataa gcgcgaaaat cgctacaata atatccgtga
   107041 caatcaaagg agctttttta tcccagcgat ccactagcgg ttctacaaat gggcttagta
   107101 aaaccatggg aatcattcct agtaaagtag caaaactcaa aattgtcgcc gaaccggttt
   107161 ctcttgtcaa ataccagatg atcgcatatt ggacgaccat actagtgatt cctgacaaaa
   107221 actgtcccga taaaaagaac aaataatttc tcttccaatt cgttaaaacg accgactgtt
   107281 cattttccat aaacatccct cttttctttt tcattttgta tagcgtttgc acaacaatca
   107341 ttatacggga ttcttttttc aaaaaatacc tttttcccta aataacaatc gtttcaattt
   107401 aaatttcttt acttagaaac aataaatcag caaaatatta aaaataacaa catgtgttaa
   107461 atttaaaaat aaaaaagaca actaatttgc gttgtcctta caaagttagt catctaacaa
   107521 taggccgttt caaaactagt tgtcttttta tatccacaga tcattcgact ttttcgaata
   107581 acccacggct aatcatccca tccatcagaa aataaaattt atcttggctc attttctgtt
   107641 ttggatcttt gaaaatattg actgctttca atccatttgc tgtagtgatc gacattttca
   107701 atgtttgaat atcttttgat acagtgattt ttaatttgaa tccttctttg ctttgcggcg
   107761 tggcatctaa tggtcggatc aattgataat tgcctccgtt tgtttctgta aatccattat
   107821 cgcgcaatgt atatcttttt gcatcaggat gcaaacgata gctaattggt gcgcccagta
   107881 ccgttgctgt tgtttcaaat gccatgattt tcacctcgat aaaattagtt tctctttatt
   107941 ataaaagttt atcctgattt ttactaggtt tttcagttaa gttccctaaa aaatgaaggc
   108001 gttctctcag acatctgttc atataagaat gacttcacta gttatttttt gacagcacga
   108061 actaattgtt caaggaaata ctggatgtcg tcttcggtat ttcccaaacc aaagctgata
   108121 cggatggatt cttttagtat cggtgtatct tctccataca ttgcttctaa tacgtgtgaa
   108181 ggctcgatat tacctgctgt acaagctgaa ccagtagaaa tagcgacccc attaagatca
   108241 agcttcatca gtagcaaatc atttgcttcg ccaattaagc gaagattcaa cacatgaggg
   108301 agtttattcg ttaaatcacc atttacttga taagggattt cgtttgcttc taatgtatca
   108361 agaatcaatt gctggaatcc tttatatttg ttgttacgtg cttcacgttc ttcaggtgac
   108421 agcagttcaa ctgcttttgc cataccccag atccctgcta aattttctgt acctgcacgc
   108481 cgcttttctt cctgctcccc gcctaaaagc aatgcaggga tatttactcc gtctcgttta
   108541 tataagaaac cgacgccttt tggcccattg attttatgag cagagatact taataaatcg
   108601 attcctaatt cttctggatg gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg
   108661 aaataagctg gatgatccgc taaaagttca ccgatttctt tgattggcat caaatttccg
   108721 atctcattgt tcccatacat cacagagacc aagatcgtat cttctcgtaa cgcttttttg
   108781 acctcttcga tcgacagatt ccctttttca tctacaggca gatatgtaac gtcaaacccc
   108841 tttgtctcta aatatttcat ggtattaaga acagcaggat gttcgatagc tgtagtgatt
   108901 agatgtctgc cttctttttg acacgagaac gctgtttcta agatagctgt attatcccct
   108961 tctgttcccc cactgttaaa gatgatctcg tgaggttttg cttgcagact gtctgcaatg
   109021 atttgtcttg ctgtttccaa tttttcatgc gcgtggcgac caaatccatg tatgcttgaa
   109081 ggatttccaa acgtgtcttg catgattgcc gtcatcgctt caattacttg tggatgcatg
   109141 ggggtagttg ccgcatgatc cagataaata gttttcactt cattcatccc ttccaaatta
   109201 ttgctaggca cattctagca taaaatagcg aacaaaccaa agaagagctc atttttgtct
   109261 agaggacgtt tcatgggcat agaaaaaagg agctatgaca aaagtcttgt cgcagctcct
   109321 tgaagctgct cacttgtgtc acaacttctt tccctctttt ttttgtttat tcgaattctt
   109381 tttcttccag acggaataac ggactcatcg gtgtattttc atggatgcgt ttgaccgctt
   109441 ctcccattaa ttctgaacat gtaacgattg ttaagttttc tggatgacga ttttcttctg
   109501 tgaaaacaga atcagtgata caaatatctt tgattggtgc attgtctaaa atacttttcg
   109561 catgatcgga caataatcca tgtgacgcac aggcatatac ttcttttgcg ccgttttctt
   109621 ttaatacatt tgctgctcgt gcaagcgttg ctcctgtgtt caagatatca tctaccatga
   109681 tacatgtctt tccttgaaca tcaccgatca cataaccacc ctcagttgaa gcatcatctg
   109741 catgatctac gatggctaat gtgctgttca aatattctga aagactacgt gcacgctgaa
   109801 caccgctgtt ttttggcgaa acaactacga tgtcatctcc cgtcagacct aattgacggt
   109861 aataatgagc aaacaacggc atcgtaaata aattatctac aggaatatca aagaatcctt
   109921 gtacttgaac cgtgtgcaga tctaaagtca gtactcgagt tgcccctgct tcttcgatca
   109981 agttagcgat caatttcgca gtgatcggtt catgaggttt cgccgtgcgg tcttgcctag
   110041 catacccata gtaaggcaat acgacattga tcgttttcgc acttgctcgc ttcattgcat
   110101 cgatcatgat cagtagttcc atatagtagt cattgacagg tgcattcgtt gattgaacga
   110161 tatacacatg atcccctcga atactttctt cgatattgat ggaaatctcg ccatcactaa
   110221 attgcttcac gacgctttta cctaattctg ttccgaacac tttcgcgatt ttttcagcta
   110281 atggacggtt gccattcaaa ctaaaaatct tcaacgtgtc atcctgatac ttattactca
   110341 tgtagtcctc cccaaaactt ctctattctt cgatagttta acatattttt ccaagatttt
   110401 ctgttaattt cagcaaattc ttaaggaagt gaaactgttc cgttactcca actggcagga
   110461 atgactttat gataatcgcc ccagttgtcg tgtactttgt aataacgttc tcctgtttca
   110521 gtttcaaaat aagcgtacgc tgtcacccaa tgattgccat atgtactgcc aagtactttc
   110581 aaaataccaa tcatcacagg tttgccttca cgtatacgtt ttgtagctcg ctgccatgaa
   110641 cctagaagag ttgctcgtgc acgctcatga ttgccattct tctttaaaaa actagaaatc
   110701 cctgtactaa cctgaaaagg aatcgtagga agtcctagtg gttggatctg gctacgcaat
   110761 gccttcgtca aaaccaacga atcagccgta tttttcttgc gaatctcatt tggaagcatc
   110821 cactccttac gatagtcttg ataataggca aggagaactg ctgccgcgta agtaccacaa
   110881 agatagcctg agggggtagc ccatttctta tacctcaaaa aacgttcttc tggcaatccc
   110941 acccagcaat ttgcgagttc ctttcctgac cttttttctt cgatcaaagc gggaatatct
   111001 tcttttctgt tgatccatct agataactcg attggctctt cattgatcag cttccatttt
   111061 ttacaagaaa gaccctcttg ataagtatat tttgctggag aatattctac cggatgtcct
   111121 gattgatcga taatcacata actttgatct tcaaaaacca gagctaaaaa aagcaattta
   111181 ctttttcttg actgcagctg ctgagtgctg gctaattttt taattttttt atctactttg
   111241 aaatataaag gtttatccac tacttgattc agttgggatc gcctcctttt tttcattata
   111301 agtgaaggat tgaaaaaaac aaatcagttt gattgttcga attttcaatt ttttatgttc
   111361 tatttgttaa ttccttcatt ttgtaagata atgggcacac gaaacaaaaa aggagcgatc
   111421 gatgaatgaa caaagaacga ttgaaacaag atttctatga agcagtcaat gaagaatggc
   111481 aaaaaacagc aaaaatccct gcagataaac cagctacagg cggtttccaa gatttagttg
   111541 acgggatcga ccaacttttg atgggagaaa cagacgatct gcttgcccat ccagaaaaag
   111601 tgtcttcaga aaaaatgaaa catttccttg catattatca actggcaaac gattatgacc
   111661 gaagaaatga gttgcatgct caaccacttc aacctataat cgaaaaagtt gaatcattgc
   111721 agtctttcga cgaattgaat gagcaatttc cagaatggct gttggataca ttaccattgc
   111781 catttaatct ggatgtagat gcagatatga agaatgcgca aacgaacgcc ttctttgctt
   111841 atccgccttc tttattcctt ccagataaga cgtattatgc atcagaccat cccaacggtg
   111901 cacaactaat aggcgttttc tttgatatgg ctgttcagtt attagagctt gccggcaaag
   111961 aaaaaagtca ggcagaagca atcacggaac aagcagtgca atttgataaa ttgattgctc
   112021 ctcatgtaaa aagtgcagaa gaaaacgcag attacagcaa aatgtataat ccgcaatctt
   112081 ttgatacatt tattcaatac acagataaac tggacttgaa aaaacttgtc acgggtttga
   112141 ttgggacaac tccagataaa gtgatcgtaa ctgatccagt gtacttcgat catttgaatg
   112201 acatcctgac agaagctcat tttccgttat tcaaaagctg gatgatcatt aaaacagtac
   112261 gttctcttag tggttatcta tcagaagaat tccgccaagt cagcggaatc tattctcgcg
   112321 tattgtctgg aacagacgaa gcaatgcctc agaaaaaagc agcgtattac cttgcctctg
   112381 gacaattcga tcaagtagtt ggcgattact acggaaaaaa atattttggc gaagctgcta
   112441 aaaaagacgt agaacaaatg gtgaaaaaaa tgatcgccgt ttataaaaaa cgtctagaag
   112501 aaaatgactg gcttggggat gcaacaaaag aaaaagccgt cataaaatta aataacttag
   112561 gtatccaagt aggctacccc gataagatcc cagcattatt cgaccagttc aaaacagtac
   112621 ctgcagatca gggtggtact ttgctttcaa atactcttgt tttcagcaaa ctcacattaa
   112681 aagatcgttt cagcaaatgg aacaaaccag tggatcgttc agaatgggaa atgagtgctg
   112741 acacggtcaa tgcttattat catccatttc ggaatgtgat cgtctttccg gcagcgatcc
   112801 ttcaagcacc attttacagt ctagaacaat caagctctgc taattatggt ggaatcggag
   112861 ctgttatcgc tcatgagatt tcccatgcat ttgataataa cggcgcttta tttgacgaat
   112921 atggcaactt gaacaactgg tggacagaag aagatcttac acaatttcaa aacaaagcac
   112981 aagcaatgat cgaagaattc gatggtctgc cttttgctga cggaaaagta aacggaaaat
   113041 taaccgtttc ggaaaatatt gctgatgcag gcggtctaag ctgtgcattg gaagcagcaa
   113101 aaacggaatc agatctttct atcgaggaat tctttgtcaa ttgggcaacg atttggcgta
   113161 caaaagcaaa aaaagaatac caacaacttt tactgcaaat cgatgtccat gcccctgcta
   113221 aactccgagc aaacgtgcaa ccaaaaaacc ttgacgaatt ctatgaaaca tttggtatca
   113281 cggaacaaga cgctatgtat ctagcgccag aaaaaagagt gcgaatctgg taagaggttg
   113341 tgccagaagt ggtcagcttc aagaaataag aaggaattgg ggaaaattgc ttctcaaatt
   113401 tttgtgacat ttcggcttat ttttcgaaga agttacttct ggaataccat gtatcagagg
   113461 ttgtgaaaaa gccgcttagt ttcaagtagt aagaaaaatt ttgtgaaaat agctttccac
   113521 atttttgcat aaatttgact tactactgag aaactggctt ttgaacaccg tttatcgaga
   113581 ggttgtgaaa tcagcgtcct gacctgagta ttagagaaaa aacggaaaaa tagttcctca
   113641 tattttgccg tttgttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag
   113701 aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaatcaaa agcagcaagg
   113761 agaacctcat ctataaaaag atagaaggaa gtgagacatc tattggtctc atttccttct
   113821 atttttactt tcctcagtca attgttttac atcataaaag gagaaattcc acttattttt
   113881 ttcgctgttt catgacataa acaactacac cgattaacaa aattcctcca gctaatgcta
   113941 gcaacgggtt attaaattct cccgtttttg ggagtccttg agtgccattt ttttcacttt
   114001 cttttgctgt cctgtccgga tgagaaggtt ttccatttgg tgattcattt ggtgattcat
   114061 ttggtgattc atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg
   114121 ggggcacatc ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat
   114181 gagcagacca tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt
   114241 ctccttttga aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg
   114301 tttcaacgat ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt
   114361 gatttggtgt gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt
   114421 ctgcattctc cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg
   114481 gttttgtctg atttggttct tgtacgattg catactctgg atattccgaa gatggcgagg
   114541 tttctccgtc aaaaatagca cctacagata atggaagacc atccactctt tccttttctg
   114601 tttgacgatt ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat
   114661 agcgcgtaac aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt
   114721 ttgatacggt atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt
   114781 tcaacgagtg gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct
   114841 caagattctc tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg
   114901 aagaggtata aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga
   114961 taatatctgt tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat
   115021 tgatcaattc atgagggccc gttaaaaaag aaccgcccat tttgaatcca ttaccgttac
   115081 cttctccctc aattcccggt acccagccgt ttcgataagc aactgagttt ttgatgatta
   115141 cttttccgat cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat
   115201 gatacgcgat acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc
   115261 catcggcatc ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt
   115321 tatgcgcggg ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat
   115381 ttccattccg ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc
   115441 ctttttgcat agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga
   115501 acagtgtcat cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat
   115561 ttttctctgc aatcaactta atcgggtttt ctcttgtccc gttaacgcct ttgggaatcg
   115621 tcaatcctct ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg
   115681 cgtatctcaa tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg
   115741 acccttctgg tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa
   115801 gaatctcttt tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat
   115861 ctggcgtaaa actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa
   115921 attcccgatt cgcagtgata gccaagtctt ttgcgttctt aagcacctct cctgttgctt
   115981 tatttttaat tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt
   116041 ttcctgaatg attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt
   116101 cttgcccagg aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga
   116161 tcgtcatatt ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata
   116221 atttttccca gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat
   116281 aatatgcctc taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt
   116341 tcgtggccgg aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt
   116401 tttccaagaa ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag
   116461 ctggcgcagc ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa
   116521 gtcgcatcgt atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga
   116581 catattcgat ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca
   116641 aatgatcttt tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat
   116701 aggtgcaaga ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg
   116761 cttgataata ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg
   116821 tggaagagag ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc
   116881 tttctccgaa aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct
   116941 tttcattttc tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac
   117001 tagttcttgg tacttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt
   117061 cgttcacaga ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac
   117121 tgagcataag gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta
   117181 ttcctcccgc aacaaattag aaaacgttta cttaaagtat tacctaattt atttttactg
   117241 tcaataaaaa acaaaattat aataagtgta ttttataaaa agaaaaaaca taacttaagt
   117301 tgttgataac aaagagattt attaacgtaa aaagaataag atttttttaa aataacgtat
   117361 aaagaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat tttgtacttt
   117421 ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt ggattcaaaa
   117481 tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc tttgaaaata
   117541 tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca gatatctgta
   117601 catacgccgg tcaaataaat atctgtgatc tggcgttctc gtaaacggat gtctagatct
   117661 gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc ctcttgctca
   117721 tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt tcctatcaca
   117781 ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc atgcgcatca
   117841 atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc agtcagcttt
   117901 ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc aacaaaatca
   117961 tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg agatgtttga
   118021 caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat tcgtcaaagc
   118081 tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa aatgggatat
   118141 tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct aatgcttcag
   118201 ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta tcacctgtaa
   118261 cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc atttcactgc
   118321 gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct ggtttgtagc
   118381 caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg atgtcaccta
   118441 cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg ttcacgccgt
   118501 attgttggat caacagactt gtggtaatag atgctaagac ttttcctata ccggaacgta
   118561 cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct gcgcgttccc
   118621 atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc attgcgccga
   118681 taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt gctaaaaaaa
   118741 ccacacataa cattaggata acaataataa gagatttcat caaaaaagag ttccatttgg
   118801 tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt ttgctttctt
   118861 tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct gcttttcgct
   118921 gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg cttcgcgtaa
   118981 taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat agttcccagt
   119041 attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc gcttgttgcg
   119101 cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt gctaaaggat
   119161 tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcatta gcaaaacctg
   119221 gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca gtttcttctt
   119281 ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg atttttccag
   119341 ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgcttgaca agaagcagac
   119401 gatcttgttc gtcaaaagca atgatcccaa caccgccagg atgaaatacc agttctctct
   119461 tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata atttttccgc
   119521 tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc agcatatctt
   119581 ctcctgcttt ctttgtaatc ataactgctt tttttcattt ttcaattttt ctcaaaaact
   119641 ccttattagt ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt
   119701 agtccgatac tctttctagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa
   119761 agaattcaat actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc
   119821 cgtggcttct agcattggtc ttgctatttc tctttggttt ttcttttgtt ctaggacttt
   119881 tattgatagg tgtagtttta gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa
   119941 aacaagaaga aattcctgca ctgactaacg aacttgaaac tcactatgaa aaaagcggta
   120001 tgacagcgaa tgaaatttca ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca
   120061 atcaattgca aatgaatatg cagcagacag ccaaacttaa agcaatcgat ctccgccatg
   120121 atccagtcaa agcagcaaaa gctttattca aagaattggt gaaagaacca aaccgactgc
   120181 atgaagcaag tcagtttctt tatacgcatc tgccgaatct agttgatttg acaaataagt
   120241 ataatgagat caatgaccat gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa
   120301 gcgcgcagat catcgaccag ctgtcagctt taatcgcaca agattatcaa aaattcgttg
   120361 ctgaggatct agatgatatc gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg
   120421 ataataaata tgaaccagaa caaaaggatt aactcttttg ttcataaaag aagactcgga
   120481 acattcagat gatgtctggc tgtttcgagt actttttcta aagtagagac attcatgtaa
   120541 gaatcgagaa cagattcaac aaggaggaat tttttatgga tcataaaccg actgaaacaa
   120601 aagatgttac accagttaac gatacacttg aagatttatt gaacaacccg ttttcaactc
   120661 caacagatac gctgaccact tcacaacaag cggagattga tcagctccaa gagcaacaga
   120721 cagctgctcg tttaattgac aaactaccgg cggaacgcca agaacaagct aaacagctag
   120781 cagcaaaaat cgatgctaat gatgcacagt ctgtcatcag ctacggatcg gctgcccaag
   120841 caaaattaag tgaattctca caatcgatgt tgaatcatgt gcaagcacaa gatattggtc
   120901 ctgtaggaga ttccttaact gaactgatgt atcgtctcca agaagccaat cctgacgaac
   120961 tgcgtgccgg agaagggaac atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct
   121021 atgaagtgac agcaaaatat caaaagattg gtgcacaaat tgacaagatt tcagtgaaat
   121081 tagataaaga aaaagacggt ctgctaaaag ataatctgat gttagaacag ctttaccaga
   121141 aaaacaaaga ctactttgat gcactgaata tttatatcgc tgctggagaa ttgaaaatgg
   121201 aagaacttca aacaacgatc attccagaag cgatgaaaag agcagaagaa actggcgatc
   121261 agatggatgt tcagattgcc aatgattaca cgcaattcct agatcgtctg gataaacgta
   121321 cgcatgatct tcgcttagct agacagatca cgatccagca agcaccacaa atccgtttga
   121381 tccaaaacac aaatcaggcc ttggctgaaa agatccaagc atctatcgct acagccattc
   121441 cgctgtggaa aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa
   121501 ctgctcaacg ccaagtctct gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc
   121561 tgaaaatctc agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga
   121621 cccttcaaac tactcaaaat gatttgatcg aaacaatcca agagacattg cgtatccaaa
   121681 aagaaggaaa agaaaaacgt cgtcatgcag aagtagagct tggacacatg gaagaagatc
   121741 tgaaacaaaa actgttggat ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa
   121801 cgcccaacaa cagtttatta caactgttgt tgggcgtttt tcttatcttt cttctaactt
   121861 ctccattgga gtctatttct cattcatctt gctgtgtcca tagccatagc caaaataaat
   121921 gatcagtcca ataaccatta cgataccgaa cacgatccat gtcacagttt gcagacgaag
   121981 catcaataca atacaagtca agatggaaat gattggcaat agcggaacga atggcacttt
   122041 gaattctcca gcttttggtt tgccaaacat ttttctcaac tttagtaagc ctagactaac
   122101 aagcatcaaa taggcgagcg ccatgatatt cgttagttcc gctaatacat tcaatgggaa
   122161 aaatccggca aagaatacag ctgccacacc agcggcataa gtcgcttttt tcggtgtacg
   122221 atgcttttga tcgatttctg tcataaactt aggaagtaag ccatccttgc tgacagcaaa
   122281 cagtaaacga gcaagtgaat acatcatcga gattgttacc gtcagtagcg ttaaaatggc
   122341 ccctacagcg atcaccgcgc ccactgttcc atgtcctaca tacctcattg caaaagccac
   122401 tgggtcattg actcctagtt cagtatacgg aacgattcct gtcaagatca gcgtcacgat
   122461 gatgtaaagg acagtagcaa tcagtatcga accaatgatt cctcttggca catctttttg
   122521 cgggtttttg acttcctcgg ctgccattgc aacggcatca aatcccaaga aagcaaaaaa
   122581 cacaagagct gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg gctgccaatt
   122641 atcaggttta acgaaaaata ttcctacaat gataaacaac gcgatgatac caaatttgac
   122701 atataccatg gcattattca aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt
   122761 gacgaatatc actacgagca ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta
   122821 cccgccagtt aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac cttgcatata
   122881 gccagaccat ccggaagcaa cagaggaaac cgctagtaaa aattccccga tcaacaacca
   122941 ccctgtcagc caggcagcaa attcaccaaa tacaacatac agataggcat atggcccacc
   123001 aatcactggc actcgcgaag caaattctgc gaaacttagt ccagacaaaa tgacaacgat
   123061 tgcagctaag acaaatgaaa atgacagtgc tggaccagca ttttgattgg ccgcaacccc
   123121 tgtcaccaca aatattcccg taccgatgat cgcaccaatt ccaagcatga tcaaatcaga
   123181 cgtttttagt tcttttttca tctcactcgt atgattgaga ttgatctctt tcttacgaaa
   123241 caaatccatt gaagttcctc cccattttag gttggaaaac agcgattaaa ctgttttcat
   123301 ataccctaaa aggataccac ctaaaatgac taaaaggcaa ccaacaataa cataaatgag
   123361 ctcttttttc gtttttcttt cgccaaggat gaaaatccca cctaaagtag agataatgat
   123421 ccccatttgt gaaagtgaga agccaacagc taaaccaagt gatttgactg ttagaagcat
   123481 gcagatgttt cctaagcccc ataataatcc acagatcatg tttttccaca caaactgatc
   123541 gactttaact ttacggaaag caaagaagct tgcgcctaag atcattccga tactttgagg
   123601 taagataatt gacatcgcat ccaaattcgc ccagttgacg atgatggtat atacaccata
   123661 accaatcgta gagaagatca atgctctaaa acccttacca aaatcaagca ttttattatc
   123721 tgtatctatt tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag
   123781 agctaaagca ataataccgt atacataatc tttcgtttgt gtccattcat ggaagaaaac
   123841 ggctcccgca atcgtattca aaattaattg aaaaccagta gacaatggta accctacaga
   123901 aacacccatg tacttcattc catggaactg tccattttgt ccaacactcc aagctaagcc
   123961 ggataaaaat ccgatcaaga aaatccaagg ggtgattaca ggactaacaa cgaaaaagac
   124021 aactaaagaa aacagaaatg caccgatcgt cattcctagt gtttgttgat tggcatcgcc
   124081 accaatttta ccacttacta aaccaatact accccatgcc aacataggga ctaatgctat
   124141 taaaatttcc ataaccaatc tccttttatt tcacatgata agaaacttat cagcgaaaac
   124201 gttaacaaca caagcacttt ttttcgtttc taacgatttt ctaacgtttt tcattataaa
   124261 aatttaagat tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt gattcagccg
   124321 tcaaatggat acctaatttt tcggccagat tttttgctaa ataaagtcct aatcctgttg
   124381 aatgtgtttt gctaagtcga ccatttctcc cagtaaatcc cttatcaaag atccgtttca
   124441 agtcttccgc tgggataccg atccctgaat ctttaactga aaggctgact ccttgtgctt
   124501 ccttttctat gctcacaatg atgtttccgt gatccggcgt atatttgatg gcattactaa
   124561 gcaattggtt gaagataaaa gcgatccatt tagcatcggt caaaacagta tgatcctcac
   124621 caacaatctc gtagcgaata tttttttgga taaaataatt tgcctgactg cgtatcacag
   124681 gctgaatgat tccttttagc ctatattctt ggatgaggta gtcccgcgaa aaactgtcta
   124741 gtcttgcata atacaaaact tgttctacgt attcatcgat ttttgataat tcattttcta
   124801 gtaatgtata cttttgatcg tcaatatcga actcgatcga acgaactagc aatttagaag
   124861 cggaaagtgg cactttgatt tcatggaccc atgagtcaat ataatctttt tggtcttgtt
   124921 gattactgat tgctgattgc attacttctt ggtgttcacg gatcagctga ttgatatatt
   124981 cttctaccag tttttcttct tctgttcgtc caccgctcaa atagttttgc aaaggggaag
   125041 ttttttgtat gtcggctaat tttccccacc atcttctctt gctcaagtaa ccacccagca
   125101 gataaaaaat cagaaagacg ccctcaatca aagctaaata agcaattgtg ctcaaatcaa
   125161 ctttgatgtt tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa aacagccatc
   125221 ccaagatcaa tgtccactga tctttcaaat atttaaaaaa attcatctta accgctcatc
   125281 ctttcaggga acgatatatc cctgtcctac ttttgtttcg atatatcctt ggatacctgc
   125341 ctgttcgatt tttcggcgta accgattgat gttgactgtc aatgtattgt catctacaaa
   125401 tcgttcgtca tcccacagtg ctcgcagcaa tttttcccga cttaagatct ttccattgtg
   125461 cttcatcaaa ataaataata gacgatattc atttttgctc aaatcaatga tctctccgtt
   125521 gatttccata cttccgttgt caacattcaa cgtaatgcca ttgtggctca tcatttcact
   125581 agataattct gtgtagttgt aagaacggcg aagtaatgca ttgatttttg caatcagtac
   125641 atcgatttgg aaaggttttg tcacaaaatc atctgccccc atgttcatag ccatgatcat
   125701 atccatattc gtattcctgc tggagataaa aatgatcggt actttcgata cttcacatat
   125761 tttttgacac caataatagc catccaaaac gggaagatta atatctagta atacaagctg
   125821 tggttcttct ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc
   125881 aaattgccat ttctcaagtt cctccatgat caattgtcga atgatctctt cgtcttcgac
   125941 taccatgatt tttgccataa aaaatccctc catctttctt cttgctacta caatatcaaa
   126001 gaagcccttg tgaaacaagt tggcttacct ggaaaatgtc attttagcaa acgttaaaaa
   126061 aaactggaaa tatgcgattt aatcgctttt gtcttttcaa tacagcttaa atttattatg
   126121 ttattcttaa caattggtct atgatctgtt atttttcctt agttgttttt gtcttttaaa
   126181 tttccaataa caccaataac cgaaaagcgt tagcactagc aatataccta gaaggagtat
   126241 aagtaatcga taaagatggt atttttttgt ctgttctatc tttgaagaga cttcttctgt
   126301 aaagggaact cgccttcctg tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca
   126361 agtgataagt gtgactagat cctgcttttc tctgattgct aatgtatttg tttttgttgg
   126421 aaggacaaca attttctcaa cgacttcata agcgagcatc cgaccataca cttcaagata
   126481 gaatcggtca tcttgctcca tttcttcaag atccgtaaag agttttttct cagcaatacc
   126541 gctatgggcg gtaatcacgg aatgtgtact ctctcctcca gttggaaaag aggtgccttg
   126601 caggactgtc gctcctctat ctaacaaaag atcattcgtt tcatcaaata aaggcaagct
   126661 aacattgatt ttcggaatat agattgctcc gatcatatgt tgttcgtagt attcttttcc
   126721 tggatttcgg acatttctaa ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg
   126781 gattactgat gtatgagcaa gcgtctgatt tttcttttca tgcactgcta attgttgctc
   126841 atgtttttct tctttatcgc gttcaagctg cttttgataa ttctcgatcc ttctttgatc
   126901 caaataattg ctgagagcat ctgctaaaaa tggatatgta aaaactgcag ctccggcaag
   126961 aaacatcact gccataaata attgtaataa aagactgatt tgggtttgag gtctattacc
   127021 tgttctcttt ttcattttct tcaccttctt atttaaaaaa actgcagcaa tcggttttat
   127081 aaaggttgct gcagcttatt tttgttgctt atacttcaga cttcatttta aatttacggt
   127141 accagctgta cgctccaatc atcaaactta ttccgattag taagaatgcc aagagcccgt
   127201 tcccaccagt agcaggaagt gacccgcgtt tcatattcgg tactctttct ggccctggat
   127261 tttctactcc tacgatctga atttcttcat tatagcttcc ataagagatg ataaattcag
   127321 taaaggctgc ctcacctgtt ggaagtacat aa
//
LOCUS       DAFEHS010000002        90282 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFEHS010000002
VERSION     DAFEHS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.002"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            10..588
                     /locus_tag="LP327_000116"
     CDS             10..588
                     /locus_tag="LP327_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724268.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(828..911)
                     /locus_tag="LP327_000117"
     tRNA            complement(828..911)
                     /locus_tag="LP327_000117"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Leu,seq:gag)
     gene            966..1145
                     /locus_tag="LP327_000118"
     CDS             966..1145
                     /locus_tag="LP327_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724269.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1294..2511
                     /locus_tag="LP327_000119"
     CDS             1294..2511
                     /locus_tag="LP327_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL1724270.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2695..3531
                     /locus_tag="LP327_000120"
     CDS             2695..3531
                     /locus_tag="LP327_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1724271.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3607..4755
                     /gene="nagA"
                     /locus_tag="LP327_000121"
     CDS             3607..4755
                     /gene="nagA"
                     /locus_tag="LP327_000121"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBL1724272.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4976..6034
                     /locus_tag="LP327_000122"
     CDS             4976..6034
                     /locus_tag="LP327_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBL1724273.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6038..7072
                     /locus_tag="LP327_000123"
     CDS             6038..7072
                     /locus_tag="LP327_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBL1724274.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7174..7890)
                     /locus_tag="LP327_000124"
     CDS             complement(7174..7890)
                     /locus_tag="LP327_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL1724275.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7859..8644)
                     /locus_tag="LP327_000125"
     CDS             complement(7859..8644)
                     /locus_tag="LP327_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBL1724276.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8635..9384)
                     /locus_tag="LP327_000126"
     CDS             complement(8635..9384)
                     /locus_tag="LP327_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL1724277.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9682..10206
                     /locus_tag="LP327_000127"
     CDS             9682..10206
                     /locus_tag="LP327_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBL1724278.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10222..11721
                     /locus_tag="LP327_000128"
     CDS             10222..11721
                     /locus_tag="LP327_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBL1724279.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11809..12411)
                     /locus_tag="LP327_000129"
     CDS             complement(11809..12411)
                     /locus_tag="LP327_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL1724280.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12515..13471
                     /locus_tag="LP327_000130"
     CDS             12515..13471
                     /locus_tag="LP327_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBL1724281.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13776..14732)
                     /locus_tag="LP327_000131"
     CDS             complement(13776..14732)
                     /locus_tag="LP327_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL1724282.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14906..15466
                     /locus_tag="LP327_000132"
     CDS             14906..15466
                     /locus_tag="LP327_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724283.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15459..16283
                     /locus_tag="LP327_000133"
     CDS             15459..16283
                     /locus_tag="LP327_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBL1724284.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16293..16910
                     /locus_tag="LP327_000134"
     CDS             16293..16910
                     /locus_tag="LP327_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL1724285.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16928..17611
                     /locus_tag="LP327_000135"
     CDS             16928..17611
                     /locus_tag="LP327_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL1724286.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18294..19577
                     /locus_tag="LP327_000136"
     CDS             18294..19577
                     /locus_tag="LP327_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL1724287.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19593..20480
                     /locus_tag="LP327_000137"
     CDS             19593..20480
                     /locus_tag="LP327_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1724288.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20497..21333
                     /locus_tag="LP327_000138"
     CDS             20497..21333
                     /locus_tag="LP327_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL1724289.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21336..21959
                     /locus_tag="LP327_000139"
     CDS             21336..21959
                     /locus_tag="LP327_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724290.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21956..23248
                     /locus_tag="LP327_000140"
     CDS             21956..23248
                     /locus_tag="LP327_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL1724291.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23607..23704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23820..24401
                     /locus_tag="LP327_000141"
     CDS             23820..24401
                     /locus_tag="LP327_000141"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBL1724292.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24404..25711
                     /locus_tag="LP327_000142"
     CDS             24404..25711
                     /locus_tag="LP327_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBL1724293.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26127..26615
                     /gene="purE"
                     /locus_tag="LP327_000143"
     CDS             26127..26615
                     /gene="purE"
                     /locus_tag="LP327_000143"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBL1724294.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26608..27732
                     /gene="purK"
                     /locus_tag="LP327_000144"
     CDS             26608..27732
                     /gene="purK"
                     /locus_tag="LP327_000144"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBL1724295.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27745..29040
                     /gene="purB"
                     /locus_tag="LP327_000145"
     CDS             27745..29040
                     /gene="purB"
                     /locus_tag="LP327_000145"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBL1724296.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29220..29597)
                     /locus_tag="LP327_000146"
     CDS             complement(29220..29597)
                     /locus_tag="LP327_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724297.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29853..30581
                     /locus_tag="LP327_000147"
     CDS             29853..30581
                     /locus_tag="LP327_000147"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBL1724298.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30581..30835
                     /gene="purS"
                     /locus_tag="LP327_000148"
     CDS             30581..30835
                     /gene="purS"
                     /locus_tag="LP327_000148"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBL1724299.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30837..31508
                     /gene="purQ"
                     /locus_tag="LP327_000149"
     CDS             30837..31508
                     /gene="purQ"
                     /locus_tag="LP327_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBL1724300.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31509..33731
                     /gene="purL"
                     /locus_tag="LP327_000150"
     CDS             31509..33731
                     /gene="purL"
                     /locus_tag="LP327_000150"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBL1724301.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33716..35155
                     /locus_tag="LP327_000151"
     CDS             33716..35155
                     /locus_tag="LP327_000151"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBL1724302.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35178..36230
                     /locus_tag="LP327_000152"
     CDS             35178..36230
                     /locus_tag="LP327_000152"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBL1724303.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36227..36805
                     /gene="purN"
                     /locus_tag="LP327_000153"
     CDS             36227..36805
                     /gene="purN"
                     /locus_tag="LP327_000153"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBL1724304.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36802..38337
                     /gene="purH"
                     /locus_tag="LP327_000154"
     CDS             36802..38337
                     /gene="purH"
                     /locus_tag="LP327_000154"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBL1724305.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38353..39627
                     /gene="purD"
                     /locus_tag="LP327_000155"
     CDS             38353..39627
                     /gene="purD"
                     /locus_tag="LP327_000155"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBL1724306.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39749..40663
                     /locus_tag="LP327_000156"
     CDS             39749..40663
                     /locus_tag="LP327_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL1724307.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40796..42361)
                     /locus_tag="LP327_000157"
     CDS             complement(40796..42361)
                     /locus_tag="LP327_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL1724308.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42354..43805)
                     /locus_tag="LP327_000158"
     CDS             complement(42354..43805)
                     /locus_tag="LP327_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL1724309.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43819..44835)
                     /locus_tag="LP327_000159"
     CDS             complement(43819..44835)
                     /locus_tag="LP327_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL1724310.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45055..46143
                     /locus_tag="LP327_000160"
     CDS             45055..46143
                     /locus_tag="LP327_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBL1724311.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46197..47723
                     /locus_tag="LP327_000161"
     CDS             46197..47723
                     /locus_tag="LP327_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBL1724312.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47725..48930
                     /locus_tag="LP327_000162"
     CDS             47725..48930
                     /locus_tag="LP327_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1724313.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49187..50164
                     /gene="guaC"
                     /locus_tag="LP327_000163"
     CDS             49187..50164
                     /gene="guaC"
                     /locus_tag="LP327_000163"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBL1724314.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50428..51852
                     /locus_tag="LP327_000164"
     CDS             50428..51852
                     /locus_tag="LP327_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL1724315.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52313..53422
                     /gene="pdhA"
                     /locus_tag="LP327_000165"
     CDS             52313..53422
                     /gene="pdhA"
                     /locus_tag="LP327_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBL1724316.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53425..54402
                     /locus_tag="LP327_000166"
     CDS             53425..54402
                     /locus_tag="LP327_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBL1724317.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54427..56070
                     /locus_tag="LP327_000167"
     CDS             54427..56070
                     /locus_tag="LP327_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBL1724318.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56078..57484
                     /gene="lpdA"
                     /locus_tag="LP327_000168"
     CDS             56078..57484
                     /gene="lpdA"
                     /locus_tag="LP327_000168"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBL1724319.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57646..57921
                     /locus_tag="LP327_000169"
     CDS             57646..57921
                     /locus_tag="LP327_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBL1724320.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57927..58694
                     /locus_tag="LP327_000170"
     CDS             57927..58694
                     /locus_tag="LP327_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBL1724321.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58862..60691
                     /gene="typA"
                     /locus_tag="LP327_000171"
     CDS             58862..60691
                     /gene="typA"
                     /locus_tag="LP327_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBL1724322.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60952..62724
                     /gene="recQ"
                     /locus_tag="LP327_000172"
     CDS             60952..62724
                     /gene="recQ"
                     /locus_tag="LP327_000172"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBL1724323.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62838..63140
                     /locus_tag="LP327_000173"
     CDS             62838..63140
                     /locus_tag="LP327_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBL1724324.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63143..64306
                     /locus_tag="LP327_000174"
     CDS             63143..64306
                     /locus_tag="LP327_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL1724325.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64332..67760
                     /locus_tag="LP327_000175"
     CDS             64332..67760
                     /locus_tag="LP327_000175"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBL1724326.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67781..68905
                     /locus_tag="LP327_000176"
     CDS             67781..68905
                     /locus_tag="LP327_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724327.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68895..69332
                     /locus_tag="LP327_000177"
     CDS             68895..69332
                     /locus_tag="LP327_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL1724328.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69316..69654
                     /locus_tag="LP327_000178"
     CDS             69316..69654
                     /locus_tag="LP327_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBL1724329.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69773..70333
                     /gene="rsmD"
                     /locus_tag="LP327_000179"
     CDS             69773..70333
                     /gene="rsmD"
                     /locus_tag="LP327_000179"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBL1724330.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70330..70821
                     /gene="coaD"
                     /locus_tag="LP327_000180"
     CDS             70330..70821
                     /gene="coaD"
                     /locus_tag="LP327_000180"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBL1724331.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70811..71854
                     /locus_tag="LP327_000181"
     CDS             70811..71854
                     /locus_tag="LP327_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBL1724332.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72013..72741
                     /locus_tag="LP327_000182"
     CDS             72013..72741
                     /locus_tag="LP327_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBL1724333.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72857..73102
                     /locus_tag="LP327_000183"
     CDS             72857..73102
                     /locus_tag="LP327_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL1724334.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73099..73317
                     /locus_tag="LP327_000184"
     CDS             73099..73317
                     /locus_tag="LP327_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBL1724335.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73453..73992
                     /locus_tag="LP327_000185"
     CDS             73453..73992
                     /locus_tag="LP327_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBL1724336.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73985..76258
                     /locus_tag="LP327_000186"
     CDS             73985..76258
                     /locus_tag="LP327_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBL1724337.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76296..77342
                     /gene="holA"
                     /locus_tag="LP327_000187"
     CDS             76296..77342
                     /gene="holA"
                     /locus_tag="LP327_000187"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBL1724338.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77431..77685)
                     /locus_tag="LP327_000188"
     CDS             complement(77431..77685)
                     /locus_tag="LP327_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBL1724339.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78085..78354)
                     /locus_tag="LP327_000189"
     CDS             complement(78085..78354)
                     /locus_tag="LP327_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724340.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78824..79279)
                     /locus_tag="LP327_000190"
     CDS             complement(78824..79279)
                     /locus_tag="LP327_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBL1724341.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79417..81345)
                     /locus_tag="LP327_000191"
     CDS             complement(79417..81345)
                     /locus_tag="LP327_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL1724342.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(81542..82375)
                     /locus_tag="LP327_000192"
     CDS             complement(81542..82375)
                     /locus_tag="LP327_000192"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBL1724343.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(82567..83271)
                     /locus_tag="LP327_000193"
     CDS             complement(82567..83271)
                     /locus_tag="LP327_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL1724344.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83294..83920)
                     /locus_tag="LP327_000194"
     CDS             complement(83294..83920)
                     /locus_tag="LP327_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL1724345.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84246..86621
                     /locus_tag="LP327_000195"
     CDS             84246..86621
                     /locus_tag="LP327_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBL1724346.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(86645..87136)
                     /locus_tag="LP327_000196"
     CDS             complement(86645..87136)
                     /locus_tag="LP327_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBL1724347.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87323..88243
                     /locus_tag="LP327_000197"
     CDS             87323..88243
                     /locus_tag="LP327_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL1724348.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            88397..88741
                     /locus_tag="LP327_000198"
     CDS             88397..88741
                     /locus_tag="LP327_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBL1724349.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(88931..89431)
                     /locus_tag="LP327_000199"
     CDS             complement(88931..89431)
                     /locus_tag="LP327_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1724350.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            89959..90042
                     /locus_tag="LP327_000200"
     tRNA            89959..90042
                     /locus_tag="LP327_000200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89993..89995,aa:Leu,seq:cag)
BASE COUNT        29320 a        15557 c        19322 g        26083 t
ORIGIN      
        1 tttttagaca tgaatatctt atttttattg ctatttttct taattttcct tctaacatat
       61 aggaaatgtc aaaactatta tgtgcgtata ttgactgcat taccgttttt cttaaacctg
      121 attatttatt ttggtaatac aatgggtcaa agctttactt acgtcaatgg aaataaaagg
      181 gcaatgattt ggagtagtag taacctgaat aatctcttta cagagttagg aacgaaactg
      241 tcactttttt atccaggtac ttggatagct actttagtag tcttggcatt attactatgt
      301 ttaattgttg gaatttattt aagttttgac aataagaaaa cttctatttt tttagttatt
      361 ttgatgataa tgggattttg ttcaaggtta attatgggat tttctcccac agtatgggct
      421 tccggaatgc ggacgtacta tatactttat gttgttatcg ccatcttagt actaatggca
      481 gttaaagagc taatgaaaag catgagtgta caaaaaaatg aatttatgca atttggatta
      541 accgtgcttg gtatttgtac ctttataata acggtgataa acagataata tacaaaaaat
      601 ccctcgcttt agtattaggt gagggatttt actcgttttt cgattaattg ttttaacact
      661 tgcaagtttc tacgagtagc ttgtttccga ccttcatata ctcacctcag gctgtttaca
      721 tgattgtacg ttctttttgt aaaggataca gtgatatgta tataatggca taaagaaacg
      781 ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga gttgtagtgc agatgatggg
      841 actcgaaccc acacgagcta acgctcacat gaccctcaat catgcttgtc tgccaattcc
      901 aacacatctg cgtaataata acacactaat gatagcgagg ttttacatag ttgtcaagct
      961 caaaaatggg aggacttatt aagatggaga aatatgacgg agaattttca ggactgggaa
     1021 tgatcttagg tgtattgatt ggacttgcat tcggacgttt tttgcttggt tttatgctag
     1081 gaatcatttg tggaattgca atggattggg cagcgaatct atggaatgac tatcatgaca
     1141 attaagttct caacgcttgt ttgtgttttt ttaatactta cgtttttcct aaataagtgg
     1201 caaaattttc taggatggag tatgatattg aaatagtaaa taacaaaaaa tttggaaagt
     1261 tctaactttt tgaaatgagg gattggctaa gtgatgagag aatttgaaaa atccaacaaa
     1321 ttagaaggtg tcagttacga tgttcgcggt cccgttctag aagaagcgga gcggatgcaa
     1381 gaagagggca ttagtatttt aaaattgaac acgggaaatc ccgcaccttt tggatttgaa
     1441 gcaccaaacg aagtcattcg tgatttgatc atgaatgccc gtgactcaga aggctactca
     1501 gattccaagg ggatcttttc tgctagaaaa gcaattgagc aatattgcca actgaaacat
     1561 tttccgaatg tgacgatcaa tgatatctac accggcaacg gagtgagcga attgatcacg
     1621 atgtgtatgc aggggttgct ggacaatgga gatgaagtac tggttccaat gccggactat
     1681 ccgttgtgga cggcttctat tgcattagct ggaggcacac ctgttcatta tatttgcgat
     1741 gaagaggcag aatggtatcc tgatattgat gatatcaaat caaaaataac gagtcggaca
     1801 aaagctattg ttatcattaa tcctaataat cctactgggg cactttatcc aaaagaatta
     1861 ttagaagaaa tcgttgaagt agctcgacaa aataacctga ttatttattc cgacgaaatc
     1921 tatgatcgct tagtgatgga tggtctagaa catattccga ttgctacatt agcgcctgat
     1981 ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc gtgtagcagg atttcgagta
     2041 ggctggatgg tcctaagtgg agataagtca cacgtcaaag gctatataga agggctgaat
     2101 atgttgtcct ccatgcgttt gtgttccaat gtattgtctc agcaaattat ccaaaccgcg
     2161 ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag gcggaaggat ctatgaacaa
     2221 agagaatata tctataatgc aatcaatgat attcctggtc tttcagccgt aaaaccaaaa
     2281 gctgcttttt atatattccc aaaaattgat acaaagcgat tcaatatttt ggatgatgaa
     2341 aaattcgttt tggacttttt acatgaacat catattctac ttgtacatgg tggtggattt
     2401 aattggaacc agccagatca ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa
     2461 caaacagcta agaaaatgag agaattccta gcaacctatc gccaaaaatg attttaaaaa
     2521 ccaaattttt cagtaggagg gccacagaca aacacactga ggctctcttt ctagtggagt
     2581 tttcttaaat aactaaagta tattatttag ttgcaagtct tttcttgaag tttgcgactt
     2641 cctaaagtaa aatacgaata gaatgcgaat acgcatattt tggggaggat acttatggaa
     2701 acgttaatcg agtcctttgg aacgatcatt aaagagattc gaaaagaaca aaaaatgacg
     2761 caaaaaatgc tttcgcatga tatttgttca caaagtgtat taagtcggat cgaaaataat
     2821 gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc aacggctagg ggtaacgatt
     2881 gatcatgtga tgaatctctc tcgaaaaact actcggacaa acgataaatt atttgaactg
     2941 ttggatagtt gtttccaaaa acgtgattat cggaagttag aacaagtact aaaatcttct
     3001 gatatttctg aaaatctgta tcaagcaact gactttcaac gctattatta ttatctcggt
     3061 gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc tttattattt gaaagaagca
     3121 ttaagctact ctagccaaaa agaaagaatc catgtttctg acacagaaat acaactgatt
     3181 agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg
     3241 agtcgaagca tccaactgct gcatcaatca gctgatgaac gatcaaaaag tgaattttcg
     3301 caaatttttt ataattatgg aaatttctta ttccatcaaa atgaaatcga cgaagcgtta
     3361 gaacaggtga atcaaggaat ctattgggct caagagaaga atagctacta ttacctcaat
     3421 gatttatttg tgcttaaaag cttaatatac aagcgtaaag aagaaccaga aagagcagca
     3481 ttctatgaag gcttagcaca agctgtgaaa caaatttcaa aaaatttgta aatggtgtag
     3541 accattcttg acttcttctc taaataagag tactttagag gtagaaaaac aaagggagag
     3601 aatagaatga gaacttttat ctatgctgac aaattctttt tagcgtcagg tgtcaaacat
     3661 gcaggctatc ttgaaattac cgatggactt tttggtgaat acaccaaaga actacctgag
     3721 ggagaagtca acatcattga ccaaagcggc aaatggattg ctcctggttt ggtagataca
     3781 catattcacg gctatatgaa tcatgatgta atggataatg atgcagaagg catcaaagtg
     3841 atgtctgaag gtctgttatc ttgtggagtg acttcatttt taccaacaac attgacgtca
     3901 agcaaagaac gtttaaaaga tgtagctgag acaatcggca aaatgtatca ggaagcacca
     3961 ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct tcacagaaga acacaaaggg
     4021 gcacaaaacc caagttattt cggggatcct gatttggata ctttccatga atggcaagaa
     4081 gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag
     4141 tttgtcaaac aagtgactga agaaggagta gttgtttctc taggacacag taatgctaca
     4201 ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat
     4261 ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa
     4321 catgtctttt ctgaattgat ttgtgatggc catcatgtac atccaaatgc tgcgggaatc
     4381 ctaatggaaa aagcaggaca tgatcatgtc gcattaatta cagactgtat gatggccggt
     4441 gggatgcctg atggaaatta caatttaggc gaatttccag tagtagtaaa agaaggaaca
     4501 gcacgtctag aatctggcaa cttagctgga agtatattga aattgaaaga agcaattaaa
     4561 aatgttgtag actggggatt agcaacacca gaacaagcag tcatgatggc tagtctgatt
     4621 ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca
     4681 gactttatcg tattgaaccc tgatatgaca ttggaagcaa cttatctaga tggcgtggaa
     4741 cgtttccacg cataatataa tttaagttaa ccttaaaact aaacttctta agtttggttt
     4801 taaggttttt tttgtgttct agtaaaaatc actttacata agtttcaaaa gtttctttaa
     4861 tttcctattg ttcaataagt aacaatgtaa agataaaatt aaaaatgtta atacgtatta
     4921 gcaaaataaa agcttttttt attgtgaata aatgaacata attaggagga gaaagatgaa
     4981 cttatttgac atttttcgat cgactttgac agatatgtcg attgtcagtg caatcacttc
     5041 aacagttttt attatattat tagggtttgt ttgtagaaaa aaggggattt tttctgaatc
     5101 tgttggaaaa attctatcga aagtcgtctt aacagtggct ttgccagcac tggcatttcg
     5161 agctttcatg caagatatca atacagaatt actgatgcaa ggaatcaatg tcttgatttg
     5221 gggattagtt atttatatta ttcttattct agtttcaaaa ccttttttct taagctataa
     5281 aggggataag caaacaacgt tgcgtgtcct gacgatcttt ggttctacga cctttttcgg
     5341 aacgcctatc gtcagtgcga tttatggtcc tgtcggcgtg atgtattcct caattttcaa
     5401 tattggctac cgaatctttt tgtattctta tggatacata aaaatgagtg gcttgaagat
     5461 ggaagctaaa aatatcaaag cgatgttcgt caatccaatc attatcgcta cttttgccgg
     5521 ccttttcatt tggctgttcc aagggtatct gccacaagtg tcagttacag gtgcagaagg
     5581 agagataagc caagtagcgt ttttgcgaat tgaccagact gcaccgtggc tatttcagcc
     5641 gatggattat ttagctggtt taagttcacc attggcgtgg ctagcaatcg gcgcaacatt
     5701 aggtgaagta agcttcaaaa aagctgcatc tgaaaaaact tcatggtatt acagtctagt
     5761 caaagtggta tttgtacctg cattgaatat cgtgattcta gcagttttga cggcaacgaa
     5821 tattctacca gtcaattttg aagcactggc aacaatcgtt atcatgatgg ctacaccagc
     5881 tgcaacagtg gcagcagcct atgctatcag ttttgatagg gaggcacttt tagcctccaa
     5941 tgcatcctta ctgtcaacta tttttgctgt ggtactgaca cctgtatgga tcgttgtatt
     6001 acaaatgatt catggcatag gtttattttc ataagaaatg gaggaagaaa gtatgagaat
     6061 cgcatgttac ggtgttcgtc cgaacgagat agattttttt gaaaaattga atatttacca
     6121 ttatgatctc agtctttatg aagaactgtt gacacatgac aatatcgtgt cagcaaagga
     6181 ccatgatgct gtattgttga ggggaaattg tatagcagat gagaccaatc ttgcaaaaat
     6241 gcaggaatat gggattcgtt atgtttttac acggactgtg ggagtcaatc atatcgatct
     6301 acaagcggca gctgattttg gtatgacagt agcccgtgta ccatcgtatt cgccaaatgc
     6361 gatagcagag ttatctttga cttttgccat gatgcttttg agaaatactg cgtatacgac
     6421 gatccgaaca tctttcaaag attttcgagt ggatgagcaa atgtttagtc gtgaaatcag
     6481 aaattgcaca gtagggatca taggaacagg caggataggg ttgacggaag ctgttttgtt
     6541 taagggacta ggagccaaag tgatcggcta cgatattcat cagacagaag ctgctaaaaa
     6601 agtattgacg ttccactcgc ttgaagagtt attagccaaa agcgatattg tcagtattca
     6661 tgtacctcat atcccaggtg aaaacgatca aatgatcaat gaaggatttt tagctcaaat
     6721 gaaaaaaggt agcatcttga ttaatacagc ccgtggagag cttcaagata atgaagctat
     6781 tttaagagct ttgaaaagca atcatttagc tggttttgca accgatgttt ttgcaaatga
     6841 aacagaggtt tttttccgat ccttcaagcc atgggagacg attccagacc ctgctatcca
     6901 gcaattagta gaaatgtacc ctagagttct tgtaaccccg catgtaggtt caaatacaga
     6961 tgaggcatta tcaaatatga tttcgacaag tttcgagaac tttcgtgaaa taatagaaac
     7021 tggaaaaacg aaaaatgagg tatctcttcc taaagcccgt caattaagat aagaaaacat
     7081 ggatattcgt caataaaatg tttaaaaata gtccaagtaa acagttgtta cttctgtata
     7141 cttggactat tttgtatggg ataattcgat gactcaacag taatctgtga gctttttccg
     7201 cccattcttt gtcaattcat aacaggttag cggccggccg atttccaaat atttgattct
     7261 ttcgttcaat atatcttgtt ctactaaaaa attcaaatat ttcttgacgg atatacgtga
     7321 cagtgagctt ttttgagcca gttcttttgt tgaaaaaata gtatcttctt ttagaataaa
     7381 tgcacttatt ttttttaaag tcagttttga cagaccttta ggcaaatcga tgaatgatgg
     7441 tatttcgtga atgggagtgg tgtggttgaa tacttgatcg atcaatttct gatcggcttt
     7501 ttcagtcgtt tggattgtct gagagaaagc aagatacttt tcgatcgcca gctgaaaacg
     7561 ttgcaaagtg aacggtttga tcaaataatc taaggcaccg tagcggatca cattctttat
     7621 ggtttcgata tcatctgcag cagtgatcaa aatcactggg atatcatttc cttgctgtct
     7681 aagccaagat aaaaaatgga taccactttc gccaggcatg tatacatcaa gcagcagcag
     7741 atctgcgttt ttttgtctga aatatttttt tccttcttct acatttctga aactttcgat
     7801 tacgttcata ttagggatgc tttccacaaa agtacgattc aattctgcaa ccattggatc
     7861 atcttcgatt atcaggacat ttgtcatcat tttgatcttc cttttttggt aattctagat
     7921 agacgattgt accgccattt gtttgtttgg aaaattctaa aaatccaccg tggcgtttga
     7981 cgattgaatg gatgagattc aatccaaacc cacgattgat tcccttcgtc gaaaaaccta
     8041 gcgtaaatat tttttctgag atttcttctg atatcccgac accgtcatcg cagacggtca
     8101 cgatgaatac ttcagcttct gtgtcatagt ttaaatgaat ccggacctgt ccgtttcttt
     8161 catgtttttg tagcacggct tcttttgcat tatcaagtaa gttccctaac acttgcaaaa
     8221 gtgaatggat ggagtcatcc atctccattt tagggataaa agaatcgctt tctaacagca
     8281 tttcaacaga ttgttctttg gcttcgttta ttttgcctaa taaaaaaccg gcaattgcag
     8341 gtactttgat cagacttgag acttggccaa tctctttttt ataattttga tttagctgct
     8401 ggataaaatg ggtgacttct tcatacttct tcaaatcaat caaaccagaa atgacatgaa
     8461 gtttattcat gaattcatgg gtttgagctc tcatggagtt tatatattgt ttcgtccccc
     8521 caagctcctc cattaatttc attacttctg atcgatttcg taaagttgcg actgctccta
     8581 aaaaacggtc attgctaaaa agaggtgtaa cgctagaaat agtttctatc ccatttaaat
     8641 acatggattg agtccgaacg gtcgtttgat gctcaatcgt ctctttaaac aaaacgtcat
     8701 atagattatc actgattttt ttcctacttc gaaatccaat agctgctctg tttggtagaa
     8761 aatgtttttt gcaggtgtat tgatcaagag gatagtttta tcagaagaga tggctaagat
     8821 tccttcatct acttcatttt cgataatcgt tttttcactt aactgttctg ctatttcttg
     8881 tggttcaagt ccaagcagta cttttttcat tttttgggca agaaaaacgg cgcctatgat
     8941 gcctacgcta agtccaataa tcaatgaaaa aacaatcatt ttttgtccat ctttgacttg
     9001 attgtcaatc gtgttgtttg ttaagccaac acaaactgca ccaataatct cttgatcata
     9061 ataaataggg acgaaatagc ggaagccgaa ccctaacatt ccttcttttt cagagaaaaa
     9121 ttcttgtcct ccaatcactt ttttcacatc ttcatgactt gagaaaggaa gaccgatgta
     9181 acttgggtct gcgtgagaat atcgaatgcc ttcagggtcc atgacaacga taaaatctaa
     9241 attcgatgca atcatgattt catttgttat tccttgaatc gcatgtgtcg ggtcaccagt
     9301 ctgcagcttt ttttggatga gtttatcatt ggcaacgatt ttagcaatgc tagagatttt
     9361 atctgacaca ctagatcggt tcatcgaaag aataaaattc ctgatcaata tggcagaaac
     9421 aatcaaagaa acgaatgtgg agaaacaaac gacaaggatg atcgtgttct ggagctttaa
     9481 tggttttttc ttaaaaaaag gaaacatagg ctacctcgaa agaatttatt gtgaaaattt
     9541 taacatgaaa acaaaaattt aacaaattgt caacgataaa cacaaccttc ctataaaaaa
     9601 caccactgag tcggtaaaag aagctataca gaattttcaa acattggtat gatagacgta
     9661 gaaatggaag gaggcgtgta tatgataggg ggaatcgcac ttgagacgat ttattggtat
     9721 acattgctga tttgtgcagg attggcagtc ctattgatta tttttggaga tgtattcgat
     9781 tttgatggcc cgcttgatcc gatgcttatt atcccttggt taactttcac aagcttattt
     9841 ggttttctag gtgagcgttt aacagatcaa tcatctttat ttttatttat tgctagtgga
     9901 ggcatatcga tagtgttggt atttttgctt aatttctata tattgatgcc gatgaaacat
     9961 gccgaatcaa cgatgtcgtc ttctgaaaaa actctggaag gacaagtagc tgttgtgatc
    10021 acaccgatcc ctgtacgagg tatgggagaa attcagttga aaagtgttac cggttccatt
    10081 agtcgtccag cttgttttta cagcccccaa gaacagacag ctgagcgggg aacgaaagta
    10141 ttgatcattg agatcaagga aagaatctgt tatgtcatgc cttatgaaag catgataaaa
    10201 gcttaaagga gcgtgtgtta aatggaaata ccagttgttt ttatcgcgat cgcaatcgca
    10261 gctttgattt tactgatgtt gctgatcgtc tttgtagcga agtaccagac agcaaaaccg
    10321 gatgaagcgt tgatcatcag tggaagttat ttaggaagca aaaatgtgca cattgatgaa
    10381 agcaataaca aaatcaaaat tgtgcgtgga ggtggcgcat tcgtcttgcc agtttttcaa
    10441 cgttcgaatc ggatcagtct attatcgagt aaattggacg tctccacccc tgaagtgtat
    10501 acggaacaag gcgtaccggt gatgtgtgat ggtacttcga tcatcaaaat cggttcatct
    10561 gtagaagaga ttgctacagc agcagaacag tttttaggca agacaagaga agaactggaa
    10621 aatgaagcaa gagaagtatt agaaggacac ttgcgctcga ttcttggttc tatgactgta
    10681 gaagaaatct atcaaaatcg cgataagttc agtcaaagcg tacaagaagt cgcttctgta
    10741 gaccttgcaa aaatggggtt gatcatcgtt tctttcacta tcaaagaggt cagagacaaa
    10801 aacggttatc ttgactcttt agggaaacca agaatcgccc aagtcaaaag agatgcagat
    10861 atagcagaag cggaagctct aaaagaaacg cgtatcaaaa aagctcaagc agaaaaagaa
    10921 tcacaagcag ctgaattaca gcgacaaaca gagatcgcag aatcattaaa agaaaaagaa
    10981 ctgaaattag caacctacaa acaagaacaa gatgttgcta aagcaaaagc agaccaagcc
    11041 tacaatttag aaagtgcccg agcacaacaa caagtaatcg aacaggaaat gcagattaaa
    11101 atcgtagaac gtcaaaaaca aatcgaactg gaagaaaaag aaatcacacg tcgtgaaaag
    11161 caatatgatt cggaagtgaa gaaaaaagca gatgccgatc ggtacgctaa agaacaagaa
    11221 gcacaagctc aaaaagtcaa agaagtgact gaagccgaag cagaacgatt ccgtgtggaa
    11281 gcactggcag aagcggaagc caataagaca cgtttagctg gacaagcaga agccgaagca
    11341 gcactagcaa aaggaaaagc agaagccgaa gcaaaacaaa agatcgccaa tgccttcaag
    11401 gaatatggcg aagcagctgt cttgagtatg gttatcgata tgctaccgca attgatgcgc
    11461 gaagcagcac agccgctagg aaatatcgaa aaaatctctg ttgtcgatac aggaagcagt
    11521 tcaggagaaa ccagtggagc gaaccgagtg acaaattatg ccacaaattt gctttcttct
    11581 actcaagaaa cactaaaagc gacaacagga cttgacttga aggaaatcgt agagaatttg
    11641 tcgacacgtg ggactactag ccaagtaaat atgtatgaag cgccaaaaga ggcagaacaa
    11701 aactcgatga aggaagaata aagtagtaca cttcaccgtt caatcaaaaa tggaggaaga
    11761 ctttttaaag ttcttctctc cattttttta ttttattgca aaagaaaatt agtcctcgct
    11821 atttttcttt tgtttttgga tcttagcttc tatctcttgt aaatttgttc ttagcaatcg
    11881 ttctttcttt tggagttcga ccaaatcttc tgatagttct tccttctttt ctgtttcagg
    11941 aggacgaaga aagaaattga taccgattgc aatgaatgtc cctataaaag tatctagtac
    12001 acgttggata gcatacaaag cggattcgcc ttgagggatg ctcaacgcaa tcagcaacaa
    12061 cgtagctatt gcggaaatga taccggaatt attgttaata ccatctgaca cgacgatcac
    12121 aataataact agaataggaa gcaagatcaa ttctaccaaa aaatcattgt gaaaatagct
    12181 tttgacaaaa aaataaatga tagctaatgt accacctaat gtattgccga tgatcctaga
    12241 ctttccaaaa gaaacggttg tagtcaagtc ctgacgcaaa gaaaaaacag cagataatgc
    12301 agcaatcatc gggattcctc gttgagtcaa atgaaaaaac aagatacata aaaaaacagc
    12361 taaggcactt ttgatcgtac gcatacctag tcgaaaacgg ccaaattgca tagagattct
    12421 cctttttggt ctttttacta tcatacctaa acttttgtct gatagctagt ttttcattat
    12481 tcaagtataa ttaaaataaa aaagggggaa gtaaatgcgt ataggaatgc gaacagtcaa
    12541 aacagtgatc agtgtcttct tttcgatggt cacagcgggt agcttatcgc tgttgtattg
    12601 gccagctgca gggatcattg cattattaag tgtaggaaat accaagcgct ctacattgcg
    12661 tacgggcatg aatcgaatcg cagcctttat attagctacg gcaacagcac tgttcagttt
    12721 ttttgctgtt gggtatacta tttttggctt cagtcttttt ttattcctgt tcatcccgct
    12781 tgcctctcgc ttcaaattaa ccgaagggat agtagtaaat tcggttttgg tcactcatta
    12841 tttagtggag gaaaacatgt cttggcagct gatcggcaat gaagcgggat taatggcgat
    12901 tggattagta tttgctttgc ttgcaaatgt ttacatgcca gatcgtacga agcagctgaa
    12961 ggaaaaccaa atacagatcg aaaaagaatt tcgaaatatt ttgagacaaa tggcagaatt
    13021 tcttttatca gaaaataaag aagatgtgca gatcaaatgc gaacatttac tgacattcat
    13081 tcgcgaaagc caagaagatg ccagagaaca tcaagaaaac tactggctca gacagccgct
    13141 atactacgaa acttacttct ctatgagaag agcacaggcc aatgtgatta aagacatgct
    13201 ggaaaatcta gagagaatcc agcaaccagc ttattatgga aaacatatct atggtttatt
    13261 gatttatact gcagaaacat tttcagagtc aaatgatggc agacaaattt taacacgtat
    13321 tgaagaagtt tatgtattgt atcggcaaat gcctctgccg actagtcgcc ctgaattcga
    13381 ggatcgagca gaattgttcc agtttttgca gtcttttaaa agttttattg aaatcaaggc
    13441 agaattctcc caacaaaaaa tccaaaaata gaggttgtga attcagctaa tattcaggtc
    13501 aaaaagctga tttcacaacc tcttctttaa acggtgttct cctgtcaact cctcggtcac
    13561 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgctcct tgtgactcgg
    13621 agctgaacgg taggctcaca acctctttct taaacggtgt tcaaaagctt tcctctgcgg
    13681 taataagtcc agccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
    13741 ttgatgcaga acagcttttt cacaaccttt taaaattaca tatttttcca tagaacttgc
    13801 atttgatcca agatttcgat gacatctttt gtcaagaaga gagagcgatt gggcaagtca
    13861 ccttcaatca tgtgtaccat attctttatt tcgtaattca ttgcttcaga tgtattcccg
    13921 ctttcgatga attcctttgt accatctgta aataagatct ccgcacgatc agcacgcggg
    13981 taatcagcaa tcgtcacata accgttctca aaggcaacga taccgacttt tggcattttt
    14041 gcctgaaaag tcaagctgac agcagcaagt tcattttctt tgttacgcaa gatagtgacg
    14101 gattgttcgt ccacgcctgt ttcaaaagga accattgtag aggcaactac ttcaggttga
    14161 gagctcaaaa agaaacgagc gaaagataca gcatacgtac cgatatccaa cagagctcct
    14221 ccagctaatt ctggattgaa gaaccggttt ttagggtctg gttctttgta actgccgaaa
    14281 ggtgcttgga tcatttttaa agcccccagt tttcctgtat ccattattga gcgtaactgc
    14341 tgataaagtg gcatgttgaa gattgtcatc gcttctgcta agatcaaatt cttttcttct
    14401 gctaagcgca tagcgtcagc cagttctttt ttattcatcg tgatggcttt ttcacataga
    14461 acgtgtttgc ctgcttccaa tgctgccaag atatggtcga tatgctgtct atttggtaca
    14521 gcaatgtaca cgatgtcgat ttcagcatcg tctaacattt cttgatagct tccgtaagct
    14581 tttggaatat tgtaacggtg agcgaagttt tccgcttttt ctgaagttct ggaagcaact
    14641 gctgttagtt cgctcgtctc ttgattgaag ctttcggcaa actcatgtgc gatggtacca
    14701 agaccaacga ttccccattg atattttttc atctgaaaac gctccttcat agttggattg
    14761 agtttcttgt ctgtctatta ttataacaaa aatcacgaag aataaagagg agaggacttg
    14821 aaacattaaa aaaaatgaaa gcagcgattt aagaggtgct ttttgatcaa aacttaagta
    14881 gaatagttaa aaggagggga taaacgtgca tcaagtatat attgatctta ttatgagtgt
    14941 attattagaa cagtttgaaa ctgaaaaaaa ttttttagat gaatacttgc tgatcagtcc
    15001 agaacaatgg caaaaatgga aagatggcga gaaaaatctg ccatctgaag cgatgcagaa
    15061 agtaaaaaat cttttcactg attatgaatg gatgctaacg caaaagattt tgcgtcaaac
    15121 gattattttt cccgaaaaac gaaacgttgc cgttgcagag tacaagcaaa tcaaaacacg
    15181 catcgcgcaa aaatggctta gcagcaaagc agcaaaagtc gagctgatcc ctttgaaaaa
    15241 taatcagcag gctggtagat accttgactt gaaagtaagt attcaatatg atgaatgggg
    15301 ctatgatgat attttgaatt ttcgtctgcc agcagatgtt caaaaacaga tcgaaggaga
    15361 aaaaatcgaa cttctcgatt gggtgaatga aaatctggaa gaaacttacg ttcatgaggc
    15421 gaatggggaa cgtgaagaac aacgaaagga tttttcaagt gaataaattt ttaggatttg
    15481 gagcagttgt gattatgatc gcagcaatgg gctatgttat ttttagcgat gatgattcgc
    15541 atgttcaagc cggcaatcaa gcagtcgaac gaacaagcac caaaacaact gctagtaaag
    15601 aaaagaacag tcaaacaacc agcagcaagg taaaggacaa agaaacaaaa gatcgtacgt
    15661 ccaaacaatt gccagatgtt aaaacagacg attggaagct agttttggtt ggtccagatc
    15721 acaaattaga aaaagaaatt gatgagtcta ctcaattggc atcattaagt aacggatact
    15781 tgatcgacaa acgcattgct ccagaatatg aagcatttgc caaggctgct gaagcagctg
    15841 gatttccatt agtgatggtc tcagcttacc ggtctgtatc atcccaacaa caagttttcg
    15901 aacaaaatgt ccaagatgtg atgagtcgtc aaggtgtatc agaagaggag gcgacaaaga
    15961 ttactaaaga aacgattacg gagccaggat acagcgaaca ccacacaggt ctggcagtcg
    16021 atgtcgtcga tcaaaactgg tacaacagtt atccttctac tgtattggat gcaagttacg
    16081 gagatcagcc tggagctaaa tggatcgcag ataatgctgc gcagtatgga tttatcgttc
    16141 gttatccaaa aggacacgaa gatataacta agatcacata tgaaccttgg cacttacgtt
    16201 atgtcggtaa agaaaacgct gagtatatca ccaaacacca attgacgcta gaagaatatc
    16261 tgaatgaatt aaatgaaaag taggaattga tcatggcgaa aaaaaagtgg aaaaagcaaa
    16321 agcgcaagaa tatccgttgg ccagcagtgg ttgccggtgt agcagtgata ctcttagcct
    16381 ttgttttttc cttgaagaat ctgtcttctc cttttactga taatcagcaa gaaaatgaag
    16441 cgcaatccca tcagcaattt attgaacggc ttgttccgca tgcccaagaa ctacaagatg
    16501 gttatggagt gcttcctagt atcattttag gacaagctat attagaatca aattggggaa
    16561 aaagtacctt ggctagcaaa tacaataatc ttttcgggat caaggcttat ggggatcaga
    16621 agaaagtaag cctggaaaca aaggaatttg ttaacgaaga atggataact atccaaggag
    16681 acttcaaagt gtatgattca tgggaacagt ccatggatga ccatacacaa ctgttcgtcc
    16741 aaggagtaga ttggaatcct gcgctgtatg aaaaagtgat tacggccacc aactatcagg
    16801 aagccgcaca agcattgcaa gatgcaggtt atgcgacaga ccccggttat gcgcaaaaaa
    16861 tcatccaggt catcgagacg tatcaattga atcagtatga ccggagatag caaaggagaa
    16921 gtggctgatg gcagaacaat ttacgccaga aatcatgacg gaattgagaa aagatatcgt
    16981 aagagtaccg gaaatcattt tagaagcaag cgggatcaag atatttggaa aattgatccg
    17041 ttcgatcatc tttactacgg atattgcgat catacgtaat acaaatgcga atgcagtaat
    17101 tgctgtgtat ccttttacac ctcatccagc gattactaaa agcattattg aggcagctga
    17161 cattccagtt ttctccggtg taggtggtgg tttgacacaa ggtgctcgtt cttcctacat
    17221 gagcatgttc gcagaagcgc aaggttcgtt aggggtcgtt ttgaacggtc ctacgccttt
    17281 agagacagta aaagaagttt gtcgagtagt tgatattcca gttatcagta cggtaacgtc
    17341 gaaatataca gaaatagatg aaaagttagc agctggtatt tcgattatca atatcagtgc
    17401 aggtaaagaa actgcagaag cagtacgcca ttttcgtaaa aaatatcctg aacttcctat
    17461 cattgcgaca ggtggtccaa cggaagaaag tattgctgag accattcaag caggagcaaa
    17521 tgccatcaca tatacgccac caagtagcgg agagcttttt agtcgtaaaa tggaaaaata
    17581 tcgggaaact gagaaaaaga atcatgaata acaggtgaga atcaaaagac gaggaagagc
    17641 agatagtggc tcttcctcgt cttttgtact ccagtactat ttgttcagaa ttcaaaaaaa
    17701 agagaaattt ttttgttttt ttacctgtaa tgtacataag aagagttaaa accttttgtt
    17761 attattagca ttctaacaaa aaaatgtctt gatttattga agcgctttct atagaatgaa
    17821 aaacgaataa aaactaaaaa aggcacccga caatttagta gcagctaaat tgtcggggct
    17881 tgaaaagtca gcgggaactg accaatcaag ttgccatagt caatgcttgt gcatcgacgt
    17941 gtatattgta cctaatttca tacagaatta ctagttattt ttctgttttt tagggaagag
    18001 cgcacacgaa gaagcatgct tatttagcta tgtcaaggat attgggcagt tgtctgatat
    18061 ccttttttct ttgcgtaatt atcctcccat cattgaagca agaagtgttt ttagcgatga
    18121 aagaagaatc agcactatct aatttttctt gaaagtattg agttgttcat cctaggcaga
    18181 acgataaggc aagtcttggt ttttattccc cttgaaaaag atcgtttaat aaatatgttc
    18241 tgtataaaaa tacagtatat tgaaaaataa caatggaagt agaggtgcag agaatgaaga
    18301 aaagaatgag aatcatctta gctttaagtg ttatgttatt cttcttgacg gcttgtgggc
    18361 aagaagatga caaaaaaatc acgctgaaca tggcttggtg gggttcgcaa gtccgacacg
    18421 atgcaacagt aaaagtaatc gagctttatg aagaaaaaaa tccccatgtg acgatcgatt
    18481 atgaatttta tgattatgaa ggatattaca caaaattagg tacactagca gcatcagatg
    18541 atatctggga cattttccaa ctgaacaatc aatttcctca atacatcaat cagattgaac
    18601 cattagatga atatgtggag caagggataa ttgatatttc taatgcagat gaatcctatt
    18661 tagcaacgac gacttacgac gggcaattag ttggactgtc taacggggtg aattcgtttg
    18721 cgattgctta caataaaaat atatttgaac aattaaatat ccctgaacca gcttcaaatt
    18781 ggacatggga tgaatttgaa caaatcagct atcaaatcac tgaagaatta ggaatctatg
    18841 ctatttcaag attcgaagat tttatagctg gctgtattat tcaaatacca caagttgaga
    18901 aaggattgaa tttttataat cgggaagatc tatctaacag cttgggattt gaaaatccag
    18961 actatctaac agattttttc cagctgcgta aaaatctggt agacaaaggg gcccatccag
    19021 aaccaggtgg tggagctgca agtacgacgg atctgtcttt tcaggcggtt agggatagag
    19081 aagcggcaat ggtgtttcta tcaagtaacc agttgacaga aatattgtca ggggctcctg
    19141 aagatatgga gattcagttg attaatccac ctcgcagaaa agcagatgga gaatctggta
    19201 tccctttacg ttcttcgcaa atgctgagta tggcaaaaag ctcagcccat aaagaagaag
    19261 ctgctaagtt tattgatttc tttcaaaaca gtgaagaagc caatgaaatt ttaaaaggag
    19321 aacgtggtgt accaatcatg tcgaatattc gagaacagct cgctcgtgca gatgatgcaa
    19381 ctttgcaagc gacatttgat tatttagaaa tgatttccga tatggacaac ggcgaagtga
    19441 atgtctttga tagtgctttg aatccggaaa ttcaagacca atacaatctt ttagttgaaa
    19501 aagtcattta tgaagaagta acagcagaag aagcagcaga gagtctttat gagtttgcgt
    19561 caaacattct aggatagcag caggaggaag gcatgaaaaa agaaaaacta aaacggttct
    19621 tggatcaaga caatgtggtc ggttacgtat ttgcttctcc attcatccta ggatttctat
    19681 tgataacagt gataccgatg atcttgtcac tttattattc gttcaccgat tatcgtttgg
    19741 gaaatccagt ctcatggatc ggcttggata attatataaa gatgctgagt gatgcccgtt
    19801 ttttgaactc catccgagtc acttttctct atgtatttgc atctgtacct gtgaaactga
    19861 tctttggtct attgattgct tatcttttaa cgaggaatat caaaggaacg actttctttc
    19921 gttctttgta ttatgtgcct agtttgatcg gtggcagtat ttctatcgct ctagtttggc
    19981 gggagttgtt ttctaaaaac gggttagtga atattttcct ggaacgatta ggcgcacctg
    20041 cgctttcctg gtttagcgat caaaaactgg tcatgatccc tcttgttttg atgtctgctt
    20101 ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat caaagagatc ccaggctcgt
    20161 attatgaagc ggcagagatc gatggagcga acaaatggca gacgttttac aagatcacgt
    20221 tacctcttct ttcgccgatc attttgtata acttggttat gcagacaatc gcggcgttta
    20281 tgacttttac gcaggcattt atcattaccc aaggtggtcc gaatgatgcc acgaactttt
    20341 atgctcttta tgtgtataac caagcttttt cttttaattc tatggggtat gctagtgcta
    20401 tttcgtggat catgctgttg attatgggag ctgtcacctt tctgattttc aaatcatcaa
    20461 aaaaatgggt cgttgtgtga atgagaggag gaagaaatga ataatacagt aacgaaatgg
    20521 tttcaatacg gttttatttt gctattaggt ctagtcatga tttatccggt cttatggatg
    20581 atcgcaggtt cttttaaaac ggatcaagag attatgagtg gttcgctgaa tttgattcca
    20641 gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg ggtttgctgg aatttcattt
    20701 tttactttct ttaggaattc gtttgtcatc tctgcagttt cgacgatagg gacagttctg
    20761 agctcgactt gtattgctta cgcattatcg agaatcgatt tcaaaggaaa aagattctgg
    20821 ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag tgattttgat tccgcagttt
    20881 attatttaca atcggctgaa tttggttggg acgtatgtgc cgctgatttt accacatttt
    20941 tttggacaag catttttcat ttaccagatc atgcaattca tggtcagcat ccccaaagag
    21001 ttagatgaat cagcgattat tgacggatgc agcaagtata gtgtctttac caaaattatt
    21061 tttcctttgc tgaaaccttc gatcatcacg acgatcatca tccagtttta ttggaaatgg
    21121 gacgacttca tgggaccatt gatttattta aacaagccga gatcttacac ggtatcgatt
    21181 gccatcaaat tatttgcaga cgctggatct agtacgactt acgcgagtat gtttgcgatg
    21241 tctacgttgt cgctgctacc ggtttttctg attttcttat tcttcaataa atatcttgtc
    21301 caggggatca gtacaagcgg tctgaagggc tgaatatgaa aaagccaata gaagagatgt
    21361 ttctttacaa gctcaactac tatgtctgtc ggattttttt aaccagtctt atcagtaatg
    21421 ctcttttcgc tttagttcta ggatgtttgt tcactttagg attagtgctg tctgctcgtg
    21481 ttgtctatct gatttatttt ttgatgatgt atccttctta ttatctatct gtccattact
    21541 tggcagaaaa aaaggtatgt tggaggaaag gaaaaatcaa acggatgtgt ttcatcatta
    21601 cgttgtttca gctgtttgtc attatttttg cagttgattg gtatttatta gtgggaaaga
    21661 ttggagcttg gtatctgatt ccttttctat tgtgtctgtt gctttttgta tcccatagtt
    21721 tttttagtac gctaattgat atctgcaaag aagataaagc agatagtctt gtttatttga
    21781 tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg gtttctgtgg tatttatgcc
    21841 ttttgtatgg actgttctgg tgtatacagc atgatttttt actcactgca ttactgtatc
    21901 cagggctgcc atttgtcaca gtattttgct taaaagaaaa ggagaatgac tttaaatgaa
    21961 gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt tttttttatg aggcaattgc
    22021 ctctacgtat catcaaactt gtgaactagt cggtttttgt gatgtatctc aaacaaggat
    22081 ggattatgca aatcggatac ttgtgaacaa gtatggacag aaaaaagtaa agacatgtat
    22141 gccatcacaa tttgagcaac tactggatga cgtacggccg gctgaagtca ttattacgtc
    22201 agtagatcga acgcatcatg attatatcat tcgtgcaatg aaaaaaggat atgatgttat
    22261 ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa gaaataataa cggtccaaaa
    22321 acagacggga agaagcctgc gagtcacttt taattatcgt tatgcaccgt atcacacgaa
    22381 gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg ttttctatcc acttcgaatg
    22441 gctgttaaat acgcaacacg gggcagatta tttcagaaga tggcatcgaa ataagcacaa
    22501 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22561 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22621 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    22681 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    22741 agcagaaaaa gacagtggat atattcgtga ccgcagtgta ttttccgatg atatctctat
    22801 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    22861 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    22921 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    22981 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    23041 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    23101 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23161 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23221 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23281 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23341 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23401 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23461 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23521 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23581 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    23641 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    23701 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    23761 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    23821 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    23881 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    23941 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    24001 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    24061 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24121 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24181 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24241 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24301 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24361 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24421 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24481 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24541 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24601 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    24661 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    24721 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    24781 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    24841 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    24901 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    24961 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    25021 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    25081 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25141 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25201 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25261 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25321 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25381 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25441 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25501 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25561 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25621 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    25681 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    25741 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    25801 tttgagctaa taccgagggt cagctgattg aacaccgttg aaggaagagg tcgtgagcct
    25861 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaaa agcttctcat gtttgttgaa
    25921 tattgttcct tgtgaccgag gagttggcag gagaacaccg tttatccata aaaaggaatg
    25981 tgacctaact tttgtcacat tccttttttt tatctttaat aaacacgaac aataaaatgt
    26041 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
    26101 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
    26161 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
    26221 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
    26281 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
    26341 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
    26401 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
    26461 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
    26521 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
    26581 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
    26641 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
    26701 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
    26761 cacattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
    26821 gtcattacct atgaattcga aaacgtcagt gtagaggcgt taaatgcgat tttacccatg
    26881 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
    26941 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
    27001 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
    27061 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
    27121 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
    27181 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
    27241 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
    27301 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
    27361 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
    27421 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
    27481 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
    27541 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
    27601 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
    27661 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
    27721 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
    27781 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
    27841 gcttgggcag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
    27901 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
    27961 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
    28021 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
    28081 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
    28141 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
    28201 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
    28261 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
    28321 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ttctactcaa
    28381 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
    28441 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
    28501 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
    28561 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
    28621 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
    28681 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
    28741 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
    28801 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
    28861 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
    28921 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
    28981 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
    29041 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc
    29101 agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac
    29161 ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat
    29221 taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag
    29281 ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat acgataatga
    29341 tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc
    29401 tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt
    29461 cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa
    29521 tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt
    29581 tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa
    29641 ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt
    29701 ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga
    29761 tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt
    29821 gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag
    29881 gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag
    29941 atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga
    30001 ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt
    30061 ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg
    30121 aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag
    30181 gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc
    30241 catttatcaa tgaagagcat atctatttct tacaacttgc aaagccggaa gaaatcagtg
    30301 agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg
    30361 gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg
    30421 cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg
    30481 acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata
    30541 accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata
    30601 tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca
    30661 tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact
    30721 tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc
    30781 aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga
    30841 aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc
    30901 gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat
    30961 ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga
    31021 ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag
    31081 tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac
    31141 tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca
    31201 agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg
    31261 gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga
    31321 ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga
    31381 acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc
    31441 taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga
    31501 ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct
    31561 atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa
    31621 gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct
    31681 ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag
    31741 gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag
    31801 cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg
    31861 gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat
    31921 tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag
    31981 gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg
    32041 atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca
    32101 aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa
    32161 aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag
    32221 aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg
    32281 aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg
    32341 gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta
    32401 tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt
    32461 tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc
    32521 aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg
    32581 ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag
    32641 ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc
    32701 agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc
    32761 tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac
    32821 aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga
    32881 ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa
    32941 tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc
    33001 ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg
    33061 aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta
    33121 tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa
    33181 tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa
    33241 aatcagggga tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat
    33301 tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg
    33361 aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc
    33421 atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc
    33481 tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa
    33541 gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg
    33601 ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg
    33661 gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc
    33721 ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga
    33781 cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc
    33841 aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc
    33901 tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca
    33961 tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa
    34021 atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct
    34081 tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt
    34141 gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt
    34201 gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc
    34261 tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt
    34321 gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca
    34381 gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga
    34441 cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat
    34501 cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc
    34561 gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg
    34621 ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc
    34681 ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc
    34741 tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg
    34801 gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca
    34861 tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac
    34921 gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc
    34981 agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga
    35041 tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata
    35101 tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca
    35161 gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg
    35221 tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt
    35281 taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat
    35341 taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa
    35401 tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata
    35461 tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg
    35521 atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata
    35581 tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg
    35641 atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga
    35701 tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact
    35761 ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag
    35821 aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct
    35881 tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt
    35941 ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct
    36001 catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg
    36061 agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag
    36121 taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag
    36181 tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt
    36241 ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg
    36301 attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg
    36361 tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa
    36421 agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt
    36481 agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat
    36541 caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt
    36601 tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac
    36661 tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt
    36721 agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat
    36781 tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg
    36841 attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga
    36901 acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg
    36961 tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt
    37021 ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca
    37081 atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc
    37141 gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca
    37201 gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt
    37261 tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa
    37321 gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg
    37381 gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac
    37441 ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg
    37501 atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca
    37561 gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac
    37621 atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat
    37681 gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg
    37741 ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca
    37801 caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt
    37861 gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta
    37921 caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag
    37981 ccgactccag cagaagcaaa agcattagtg tttgcttgga gagctgtcaa acatgtgaaa
    38041 tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg
    38101 aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca
    38161 gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat
    38221 ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc tattgatatg
    38281 gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga
    38341 ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt
    38401 aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg
    38461 atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc
    38521 cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta
    38581 aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca
    38641 gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc
    38701 ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa
    38761 aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg
    38821 gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt
    38881 ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt
    38941 ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg
    39001 gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg
    39061 caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg
    39121 ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg
    39181 aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc
    39241 gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt
    39301 acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc
    39361 ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt
    39421 tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt
    39481 ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc
    39541 gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct
    39601 gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa
    39661 acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg
    39721 ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca
    39781 gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa
    39841 agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg
    39901 aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga
    39961 agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa
    40021 aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga
    40081 tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt
    40141 tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat
    40201 gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt
    40261 tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc
    40321 aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca
    40381 agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt
    40441 ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg
    40501 cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat
    40561 gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga
    40621 ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg
    40681 tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat
    40741 attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt
    40801 tatttttgtt tttaaggaaa agctgtgtcc gatattcttt tggagaaatc ccttgttttt
    40861 tcttaaataa ataactgaaa tagttagggt cattataacc aatttcaaaa gcaatatctg
    40921 aaatacgata gtctgattgc tgcaataatt tttttgctaa ttcgattctt tgttctgtca
    40981 gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac
    41041 ttaatgcaac ttgttgagcg acccaagtta aggacatatc tggatcggta aaattttctt
    41101 tgataaaatc caatgcatgg ttcaaaattg gctgatattt gttccttgaa ggctgtgagg
    41161 gcagagcagt taagaaagaa atgaacatat aaactgtcga acggtaaagc gagtcgtcgg
    41221 cgctgattgc taagcgttga ctggtggatg tgcaatgaac catttcttgc gggattttct
    41281 tttctttcag gcagacatgc tggagtagct tagtcaagac aaataaacga tataatcgtg
    41341 ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat gtacttagat acttcgtttt
    41401 tcgataatga agacaaatca agtgagaggt caaagtaaaa gacagaatca actggaagtt
    41461 tcgcttcttg aatcaaatcc tcatagctga taatttgttc tttaagggaa ctggcggttt
    41521 caagtatcat ctgcgtgatc tcaaaagatg caggtaattc gctgattctt tctgcgacag
    41581 gaccaatggc gacagaaaaa ttattttttt gtcccttttc taattcatgg accaacgttt
    41641 gggctacctg ataactggtc tcgatcacat tttcttcaag agaatcaact attaaaaatt
    41701 tgatgaatcg agaggaaaca gcagagaaga tcacttgttg attctcccta aataaaaact
    41761 tcaaataatc actgagatga gcgtaatctt caaagtctgt actgacttgg tttgctgcta
    41821 ataatacggt catttttttc cctgctaaag agcgattgaa agattcagca agtttcaatg
    41881 tttcactgat cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt tttttcaatt
    41941 cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa gcagttactg
    42001 ctttttctat tgcttgtttc aattcgctac ttttaatcgg tttaagcaga taatcatctg
    42061 cctgtacctg gattgctcct ttcatataat cgaaatcgtc gaatccacta ataaatatga
    42121 gacgagtcca aggaagtagt ttccttgctt cttttgaaaa agtcaacccg tccatgaaag
    42181 gcatttggat atcagtcaga atgatgtcgg gctgtaagtc gataattgaa gacagtgcca
    42241 tctcaccatc tccagcttcg ccgacataag tgattggata ataagaacca aggacagtta
    42301 tttgttcccg caagttattg cgaatgaggt attcgtcttc gatgataaaa actttataca
    42361 tgtttgtttc ctccaattgt tttgggagat agagggatca tcagtgtgac agttgttcct
    42421 tctcggtatt tgctgtcaat cgtgataggt ttttgatcat ttccgtaata aagaaggaga
    42481 cgcttgttta cgttgtaaag tccgtagccg atactgaaat ctgaatcgat ccctttggct
    42541 aactctttac gtatttctgc cagtttttct gcagggatgc caatcccgtt gtctataact
    42601 ttgaattgga tcatctcatc aattttgcat gtggtaatac gaataaatcc tttgtgacgg
    42661 acgtacttcg ttccatggta tacagcattt tcaatcaatg gctgcaagat catttttaaa
    42721 atagaaacag gaaaaagtgt ttctggtata tcgatcttat agtcaagcat atggccgtat
    42781 cttattttta agatgactag ataatcattg atatggctaa tttctttttc aagaggaatc
    42841 cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc
    42901 agagcttctt caaagcagtc ttgttcgatc aaagaaataa tggcatccaa actgttataa
    42961 ataaaatggg gagtaatctg agcttgcaat acacgaactt cactttgtgc taaatggtat
    43021 tgtttcaaag cgttctcccg taaagactgg tttagatctt ccgccatttt attcatgcta
    43081 gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc aaggttccct
    43141 gaagaaagtt gttctgacat ctcgatcagc tgattgatcg gttcttggac ctttctctct
    43201 aaaaagcgac gattcttttt cgcatagaaa aaaatggtac taagaactcc aagttccact
    43261 attgttaata gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc
    43321 tccacacgaa caaaatcttg taagatatct gacagcagtt gagtgacaga atctacctga
    43381 ttgatgatct cttcattggt atccataaaa tcttttgcca agatattatt gatcagctcg
    43441 ttttgataat cttccagtgt atcgatcgta cgtagcgata catctaacgt actaatttct
    43501 ttaagtgtat ttgtattatt tttgatctct cgaatatcat tcctaagttc ttctaggata
    43561 ttttcctcat tgaactcatc tacggttact tggccagaaa tcaagtccca catactttcc
    43621 aaaacttttt gatcaacctt caaagcaatt gcatctgctt tttggatatt tgcgatcgtc
    43681 tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa agggataaca
    43741 gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct gttttatgct taaatttttt
    43801 gtcatgatac ttctccagtc aataatttct ggttgggatg ttagctatat tctgctggga
    43861 aaagacagtc tcggaaataa aaatatcttt aggtaatgtt tttcctgttt gaatatttcc
    43921 aaaataacga gtaacggtat tggctacata ccagccgaca tcaggtttac actcgactac
    43981 acaattgacg attcctgagc gtagcttctc gatcatatct tcttgtccat caatagttac
    44041 aatgatcaaa tcttctccag gtttgattga tgtttctttt atcgcagcta aagcaccgtg
    44101 agtcatttca tcactatggc tgtataatac atcgatttcg gataaatcat ttgtttctaa
    44161 atatcgtttc atctggtctt ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac
    44221 aatagtcatc ggaggtttat ggtcgatggt tccatctttt tttagggttg gtttacgatt
    44281 gattgattcc aaaaatcctt cggagcgtag cttggtcggt gtggagttct ctgaaccagc
    44341 aagttctagg attctgattt cctgtttctt tgaatttgtg aagtgattgc tgacaaataa
    44401 acccgcccga tttccttctg ctttgaaact ggggccaatg tgggtgacga aaagctcggg
    44461 atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg atccctgctc gctttgcttc
    44521 ttttaataca gagtcccacc cggattcttg caaaggagta aaaatgatca tatccacttt
    44581 ataggcaatg aacgtccgta tatcttggat ttgccgttcc tgattcatgt agccatttcg
    44641 gtagagaact tggtaatctt ctttttcaag cgcagtgata attgattcag tatgcttttt
    44701 ccgccagcta ctctccgttc ctgattgcga aaaaccaacg actagtgggt caggtgcttc
    44761 ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa
    44821 taaaacacgt ttcactttaa atcactccat ctggatttgt tagtactatc ttatcagata
    44881 aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat
    44941 ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt
    45001 aacgctttct ttattagcta tactcggtgt atcaattgaa atgggggata aaaaatgaaa
    45061 aacaaattta gaaagattgt tgggactatc gctattttga ccgcaagcat tctattaagt
    45121 gcgtgtggca attcaggatc tgcagatgat agtacggggt atgtaggaat cgctatgccg
    45181 accaaatcag cagaaagatg gatcgctgat gggaacaaca tggtctcgga attagaaaaa
    45241 ctgggttata aaacagatct tcaatatgga gaagataaag tagaaaatca agtagcacaa
    45301 atagaaaata tgattacaaa aggggtagat acacttgtga ttgcttctat cgatggttcg
    45361 gctttaactg atgtgttagc taaagcaaaa gaagcagata tcaaggtgat cgcctatgat
    45421 cgtttactga tgaattcaga aaatgtcgat tattacgcga cttttgataa ttttggagta
    45481 ggtgtatcgc aagcagctta tattgaagag cacttaggat tgaaagaagg aaaaggacca
    45541 ttcacgattg aattatttgg tggctcgcct gacgacaaca acgcactaat caattacaac
    45601 ggagtgatgt ctgttttaca accatatatg gacaacggtc agttggtcgt gccatctgga
    45661 cagacgagtt tcagtcaaat cgctacgctt cgctgggatg gttctacggc gcaagcaagg
    45721 atggataatc tgttaagcgc aaattatact gaccaaacat tggatgctgt tttgtcgcca
    45781 tatgatccaa taagtttagg aatcatttcg tcattaaaag gcgtggggta tgggagtgaa
    45841 agtaaacctt tacctgtgat tactggacaa gatgcgacag ttgctggcgt taaatcgatc
    45901 attgccgggg aacaaacaca aacgatcttt aaagacacac gtatcttagc aaaaaatacg
    45961 attgaaatga tcaaagcaat cagtgatgga gaagaggttc cggtcaatga tactgaaact
    46021 tatgataatg gggtgaaaac tgtccctacg tatttagcaa atacagtatc tgtcgataaa
    46081 gataattatc aagcagagct gatcgacacc gattattata aagaatccga tctgaaaaat
    46141 taagaggaac caaacaagga actaaagaga tctaagtcag agagaggata agaattatgg
    46201 cggattgtat tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc cgagcgttag
    46261 attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc attgtgcgga gaaaacggag
    46321 cgggaaaatc aacattgatg aatgtcttga gcggcttgta tccatatggc tcctatgacg
    46381 gggaaatctt ttatgatgga aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa
    46441 aaggcatcgt catcattcat caagaattag cactaagtcc ttatctttca ataaaagaga
    46501 atatttttct cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa
    46561 agaaaacata tgaactgctg aataaagtcg gtttgtctat cgacccgaat cttctagtat
    46621 cccagattgg cgtagggcaa cagcaactag ttgaaatagc taaagcatta tcaaaatccg
    46681 tccgtctgtt gattttggat gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt
    46741 tgctgagatt gatccgagag ttgaaagagc aaggtgtaac ctccatcatt atttcacata
    46801 agctgaatga aatcgtggat atcgcagatc gaatcaccat tttacgggat ggacagacga
    46861 ttgagacact agaaaatgaa atgatcagcg aagagcatat tattcgcgga atggtgggca
    46921 gagatttagt tgatcgttat cctgaaagaa aaccggatat tggagaagtt tattttaaag
    46981 tagaagactg gacggtctat caccctcttg atcacgagcg aatcataaac gaccacatca
    47041 acttgcattt gaaaaaagga gagattgtag gaatagctgg actgatggga gcgggcagaa
    47101 ctgaattcgc tatgagtcta tttggacatt cttatggaag caagatatcc ggacgtatat
    47161 taaaggaagg ggcagagata tcagttaaga ccgttccttc agccattgca aacggactcg
    47221 cttatgtctc ggaggaccga aaatctttag gactgaatct gctgatggat attcgggaaa
    47281 acgtgacgct gagcagtttg gataaaataa gtcaaaaagg agtattagat aaagaaaaag
    47341 aagtaactga ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac tccatccatc
    47401 aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc aagtggctga
    47461 tgacacaacc cgatattctt tttttggatg agccaacccg tgggatagat gttggggcaa
    47521 aatatgagat ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta
    47581 tctcttctga acttcctgaa atattaggga tgtgtgatcg aatctatacg atgaacgaag
    47641 gacgtattac aggagaagtc tcgcgagaag atgcagatca ggaagtcttg atgaaattaa
    47701 tgacgaaaga agaggtgggt taagttggaa caggcaaaaa atcttacgga taaaaaagag
    47761 aaaaaactaa atttgaaaag tagagcagct gatctttttg gtcgctacag catggtgatc
    47821 attttagcgg gactacttat tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca
    47881 ttgaatataa ctaaccttgt tctgcaaaat agccatatat tagtactagc tgcagggatg
    47941 ttattagttg tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt
    48001 ggtgcaattg cgggcatgat gatggtcaat cgccaaatga acccgtggat gacagttatc
    48061 gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag gtttttgggt agcttatgca
    48121 gggataccgg cgtttatcgt gactttagca ggattgctga tgtttcgtgg actgacacaa
    48181 gttgttctag gaggccaatc tctagcacct tttccagctg ttttccaaaa aatgtctacg
    48241 ggttatttac cagacttaag cgaaggaagc agccttcact tcttgacatt gattttagga
    48301 atgatcatca cgatcgtgtt agttgttggt caatggcgaa caagaaatcg tcggaaaaat
    48361 catttatttg aagtggaatc tccaacagct ttcttggtaa aggcggtatt tacagcagtc
    48421 gttgtactag gaataactta tatctttgcc tcttatcaag ggtatccgat tattttgatc
    48481 attcttggtt tgatcgttgc agcatatggg tttttgacaa ataaaacagt agcaggtcga
    48541 cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat caaaacaaag
    48601 cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg cagctttagc tggattagtt
    48661 ttagctgctc gattgaatgc ggcaacacct caagcaggta catcgatgga actggatgca
    48721 atggcggcag tatattttgg tggtgcttcg acttccggcg gcattggaac aatcatgggt
    48781 gcgattgtcg gtggcttggt catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc
    48841 ggggtagact ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt tgtgctagac
    48901 ttgtataata agaaaaaacg aacaacgtag gagaacgccg gttattcaga aaaaagactg
    48961 tgacaagaca tttgtcacag tctttttttc gctaaaagcg aacattcatt gtttcgtttg
    49021 tatagactaa aaataatata tgttttttta ccattcgtat aaattctctt tttgctcttg
    49081 atttttttgc ttaattgagt taaattacat agggcgctaa aaaaagcctt gattaaatta
    49141 ataatgttcg tgttttgctg gatgtgaaca tcaggaggat aatgtaatga aagtatttga
    49201 ttacgaagat attcaattaa ttcctaataa atgtatcgta aatagccgtt ctgaatgtga
    49261 tacaacggtc acattaggta aacatacatt caaaatgcca gtagtgcctg ccaacatgca
    49321 gacgattatt gatgaaacaa tcgcagaatt cttagcggaa aatggttatt tctatatcat
    49381 gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt
    49441 gattacctcg atcagtgttg gcgtcaagaa agaggaatat tcctttatag agaagttggc
    49501 agaagaatcg ctaaatcctg attatatcac tatcgacatc gcgcatggac atgctaactc
    49561 agttattgat atgattcagc atattaaaaa atatttgcct gaaacttttg tgattgccgg
    49621 taatgttggt acaccggaag ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa
    49681 agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc aagacaggct ttgggactgg
    49741 aggctggcaa ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc
    49801 tgacggtggg atcagaacac atggcgatat tgcaaaatct gtccgttttg gtgcgacaat
    49861 ggtcatgatt ggttcattgt ttgccggtca cgaggaatct cctggcgaaa caaaagtcga
    49921 aaatggaatc gtgtataaag aatactttgg ttctgcttca gaattccaaa aaggcgaaaa
    49981 acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa ggatctttga aagatacact
    50041 agtggaaatg caacaagacc ttcaatcatc tatttcttat gctggaggaa gagatctgga
    50101 agccattcga aaagttgact atgtcatcgt gaaaaattcg atttttaatg gagacacaat
    50161 ctaaaaaata aataaaatat taggatgttg tgaaaaagtt gtcctgcatt aagtaataag
    50221 aacagccaaa caaaaatagt tctttatatg ttttgtttgg ctggaattat tatcgcagat
    50281 acaagctttt gaacatcatt attcagaata agggactgtg ataagatttt tgtcacagtc
    50341 ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa
    50401 aaaatagaca atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg
    50461 atttatcgtc aaattatgga acagattatg agagaaatcg aaaacggaag gatcaatcct
    50521 ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc
    50581 gttgtccatg cattggaaga gcttagggag cttggggtac taacgagcaa acgtggcagc
    50641 ggatggtttg ttaatcagac tgaatggggg aaattcgctg ttccaagaat cgattggcgg
    50701 caaatgatct ctcctcgtta tgagcagacg gactggtatg agcaaaaaat aagagaagca
    50761 aagcagatcc aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc agatgagctt
    50821 ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa tcttagcgga agaaaaagaa
    50881 atggatcttt taggctatac tccgttacgt aacaaaatca aacggtattt ggaaagtaaa
    50941 tttgaattgg cattttcccc caaccagctt cttgtgactg gtggcggtca gcaagcaatc
    51001 tttttgattt tacaaacgat tctttcagct ggagagggga tagctgtaga gtcaccttct
    51061 tttttttacc gattacctct tttcaaagct tcaggaacgc gtttatttgg gataccgatg
    51121 gatgaagaag ggatcgactt agaaaaactg aaagaatcga tccataaaaa taaattgaaa
    51181 gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga
    51241 cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc caattattga agacgatgta
    51301 tttacggatc tttcatttct aaaccaagaa gaagtccttc ctatacgaac gattgatccg
    51361 gataatgtgt tatacgtcgg ttcgttatct agggttttag gaaaaacaac gaaaatcggt
    51421 tgggtgatag gacctgagac ttttatctcg caactagctc aagcgcaaga aatgatggag
    51481 ttttcgatga atatccttac acaaatcaca gtggctaatg tatttaatga agcaattgat
    51541 ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg
    51601 ggagattcac aatcgttttt caagcttcat tcaattttag gtggttacta tgcctggatt
    51661 acttgggatg ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga tatgggtctt
    51721 ggaattgcac caagtctact ttttgggcaa gatataaatg gaatcagaat caattacagt
    51781 aggctggatg aaaaccaaat accgttgttt tattcaaaga tgaaaattct agaaaagtgg
    51841 ttaacagaat aatttcacaa agtaaataga ttaaagaata taacagcttg gatttaaatt
    51901 tcaagctgtt tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca
    51961 ttatttgtac gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata
    52021 acaccaacgt ttttaaatag aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat
    52081 gcagatgaaa tataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg
    52141 cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata
    52201 aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt
    52261 caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa
    52321 caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt
    52381 atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc
    52441 cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg
    52501 ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga
    52561 ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta
    52621 tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc
    52681 tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat
    52741 tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa
    52801 caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta
    52861 cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa
    52921 cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa
    52981 agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata
    53041 tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga
    53101 aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg
    53161 tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata
    53221 tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa
    53281 agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga
    53341 tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta
    53401 tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat
    53461 gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg
    53521 aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga
    53581 gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta
    53641 gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac
    53701 gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc
    53761 atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat
    53821 ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat
    53881 gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa
    53941 cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct
    54001 ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca
    54061 atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa
    54121 atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa
    54181 aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa
    54241 gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt
    54301 tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca
    54361 aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac
    54421 aaagtcatgg cttatcaatt taaattgccg gatatcggtg aaggaatcgc agaaggtgaa
    54481 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    54541 gttcaaaatg acaaatcagt agaagaaatc ccgtcacctg ttacaggaac ggttaaaaat
    54601 gtgattgttc cagaaggtac cgtagcaaat gttggggatg tattagtaga aatcgatgca
    54661 ccaggacacg aagacaatga aggggacagt ggtgtagccg cagaatccca aactccagct
    54721 aaaccggctg ctgaacccac tgttgataca gaatcagctg gatcgtcaag cgaaggagtc
    54781 ttccaattta agttaccgga tatcggtgaa ggaatcgcag aaggtgaaat cgtcaaatgg
    54841 ttcgtcaaac caggagatac gatcaatgaa gatgatacat tactagaagt tcaaaatgac
    54901 aaatcagtag aagaaattcc gtcacctgtc acagggacag ttaaaaacgt gattgttcca
    54961 gaaggcactg tagcaaatgt tggggatgta ttagtagaaa tcgatgctcc tggacacaat
    55021 agtgcaccgt caactagtgc gccatcagct gaagcaccaa aagaaaaagt ggaaacatca
    55081 ggttcagcaa gtgtcgttga agctgccgat ccaaataaac gtgtattggc tatgccatct
    55141 gttcgtcaat ttgcccgtga aaaagatgta gatatctcac aagtaactgc aactggaaaa
    55201 ggtggcagag taacaaaaga ggacatcgaa aacttcttgg caggtggtcc aagcagtgca
    55261 ccagcgaaat cagaagcgcc agaagcggca gcaccgaaag aagcagctcc tgcagcagaa
    55321 agcaaaccag ctgcaccagc aaaaccattc aagtccaact taggagactt ggaagaacgt
    55381 gtagcaatga cgccaacacg taaagctatc gctaaagcaa tggtcaacag taagcataca
    55441 gctccacacg tgacattgca tgatgaagta gaagtgtcta aactatggga taatcggaaa
    55501 cgatttaaag aagttgctgc tgcaaatggt acgaaactta ctttcttgcc atatgtcgtg
    55561 aaagctttaa cagcgactgt taagaaatac ccagtattga atgcatctat cgacgatgca
    55621 aaccaagaaa tcgtttataa acattactac aatatcggta ttgcaactga tacggatcac
    55681 ggactatatg taccaaatgt gaaagatgca gaccgcaaag gaatgtttgc tattgcagac
    55741 gaaatcaatg agaaagcaaa attagctcat gacggcaaac tatctgcaga agatatgcgc
    55801 aatggaacaa ttacgatcag caatatcgga tctgtaggtg gcggttggtt tacaccagtc
    55861 atcaactatc cagaagtagc tattttaggt gtaggaacga ttgcacaaca accaatcgta
    55921 aatgctgaag gcgaaatcgt tgtcggtcgt gtgatgaaac tgtcattaag tttcgatcac
    55981 cgtatcgtag atggagcaac tgcgcaacaa gcaatgaata atatcaaacg tcttttagct
    56041 gatccagagc tattaatgat ggaaggatga taaacaaatg gtagttggag attttgctgt
    56101 agaattagat acagttgtaa ttggtgcggg acctggtggc tatgtagctg ccatccgcgc
    56161 tgctgaaatg ggacaaaaag tagcaatcat tgaaagagag tatattggcg gtgtttgttt
    56221 gaatgtcggc tgtattcctt caaaagcact gatagctgca ggacaccatt atcaagagtc
    56281 actagattct gaaatgtttg gagtaactag tgaaaacgtc aaactagatt ttgcaaaaac
    56341 acaagaatgg aaagaaaaca aagttgtcca tacgttgact tctggtgttg gtttcctatt
    56401 aaaaaaacat aaggttgaaa caatcgaagg agaagcgttc ttcgtagatg atcacacact
    56461 acgcgtcatt catccagatt cagctcaaac ttattcgttc aataacgcaa tcatcgcaac
    56521 aggttcccgt ccaatcgaga ttccaggatt taaattcgga ggacgtgtgt tagattctac
    56581 tggcggatta gcattgaaag aagtacctaa gaaatttgtg atcatcggtg gcggtgtgat
    56641 tggtgccgaa ttgggcggag cgtacgcaaa cttaggagca gaagtaacga ttcttgaagg
    56701 ctcaccacaa attttaccaa cttacgaaaa agatctagta aaattagtag aagatgattt
    56761 caagaaaaaa ggcgtaacag tcgtaacgaa tgcaatggcg aaagaagcag tcgataacgg
    56821 tgacagcgtg acagtgaaat atgcagtgga cgggaaagaa gaatccgtta ctgctgatta
    56881 tgtgatggtc actgtaggtc gtcgtccgaa tacagatgat atgggcttag aacaagcggg
    56941 tgtcgaagta ggagaacgag gattgatcac ggtggataaa caaggacgta cgaacgtacc
    57001 taacatctat gccatcggtg atatcgttcc aggggcggca ttagcacaca aagccagtta
    57061 tgaagcaaaa atcgctgctg aagcaatctc tggcaaaaaa gtagctgtag attataaagc
    57121 aatgccggca gtagccttca ctgatcctga attggcttct gtaggtatga cgatcaaaga
    57181 agcaaaagat gctggtatcg aagcaaaagc atataaattc ccattttcag ggaacggtcg
    57241 ggcattatcg cttggtaaaa cagaaggatt catccgtcta gttacgacga tcgaagataa
    57301 cgtgcttatt ggcgcgcaaa tcggtggagt cggcgcaagt gatatggtct ctgaattagc
    57361 tttagcaatc gaatcaggaa tgaatgctga agatatcgcc ttgacgattc atcctcatcc
    57421 atctcttggc gaaatcgtca tggatgcttc ggaattagca ttaggtttac caatccatat
    57481 ttgatttgaa taatacaggc gaaacgcaac tgaaaaatca gttgcgtttt gttttttaga
    57541 attatcaata atattattat gtaaacttaa aaatatgaca acttcttggt ttattaccac
    57601 ttcctatgct acactgaaga ggaatatgaa ataggagagt tgaagatgca agaatatcag
    57661 tatccacttg atttagattg gacaacagac gaaatggtca ttgtaatgaa gatgtgggaa
    57721 gatctggaac gtgcgaatga aaaagggata aacaatgaag cttttttgaa gacttatcag
    57781 cagtttaaaa ctgttgtcaa atccattggt gaggaaaaac gattgggacg ggaatttgaa
    57841 aaagcttccg gttattcttt atatcgtaca gtgcagcaag caaaaaaaaa tccaaacaaa
    57901 actttgagaa tgaacgggtg agataaatgg aacagatgat tgatcatatc aagtcctggc
    57961 ttgaagaagc tggtgagaaa atcaaatctt ctcaacaaac attaacaatc tcgcaaaaat
    58021 caaatcgaac ggatctagta acaaatgtcg atcgaaacat ccagaatttt ctaatcgaaa
    58081 aaatccattc ctttgatcca gaggcgaaaa tattggcaga agaagaaggg tgtaatcaat
    58141 tatcggatct cgatggaaga gtctttatca tcgatcctat tgatggcacc ttgaattttg
    58201 tgattgaagg ggaaaatttt tgtattatgc tcgctgttta tgaatccggc gttggaaagc
    58261 ttggtttcat ttatgacgtg atgagaaatg aacttttttg gggtggaaaa ggaataggcg
    58321 tgtataaaaa tgatgagcca ttgcctagac cagcaaataa atccttatcg gaaggcttac
    58381 ttggtgtgaa tagttatctt ttcggtcata ataaatatgg aatccgagaa attgcggaaa
    58441 gcagcatggg gacacgtatg tacggatgcg ctggtcttga actagtggca atcttgaaag
    58501 gaaaccacat tgcttatatt tcaaacctta gcccttggga ttatgcagcg ggaaatgtct
    58561 tattggaaga gtttggtatg gtaacaagcg gtctttttgg tactcctttg acatttgaaa
    58621 aaagggaata ctatttagca gggacttctt gtgcatatgc agaaatcgca gaccttttga
    58681 aaaaagaagg ctgaaaaaaa ggtagaaatc attcacaaaa atgataaaaa cgagcaaaag
    58741 attaattttc tcgaaaaaat gaaaacattt tactatattt catgaaaaaa gtctgctata
    58801 attagcagtc gagtaaaatt tgccctgtca taaagaacca ataaaaaagg agttagttac
    58861 attgaattat agaaacgata tccgtaacgt cgcaattatt gcccacgttg accacggaaa
    58921 aacaacatta gttgatgaac tattaaaaca atcagataca ttagacgcac atacgcaatt
    58981 gcaagaacgt gcgatggact caaatgcgct agaaaaagaa cgtggtatca cgattttagc
    59041 caaaaacaca gctgtagact ataaaggtat tcgcgtaaac atcatggata ctccaggaca
    59101 cgcggacttc ggtggcgaag tagaacggat catgaaaatg gttgacggcg ttgtgttggt
    59161 cgtagatgcg tatgaaggaa caatgcctca aacacgtttt gtattgaaaa aagcactgga
    59221 acaacatatc acaccaatcg ttgtcgtaaa caagattgac aaaccttctg ctcgtcctga
    59281 gcatgtcgta gatgaagttc ttgaactatt catcgaatta ggagcagatg atgatcagtt
    59341 agactttcct gtgatttatg catctgcact aaatggtacg tcaagcttgt cagatgatcc
    59401 agcagatcaa gaaccaacca tggcacctat ttttgataca attattgaaa aaatccctgc
    59461 tcctgtcgat aacagcgacg aaccattaca attccaagtt tcattacttg actacaacga
    59521 ttatgtaggt cgtatcggaa tcggacgtgt atttagagga acaatcaaag taggagacca
    59581 agtagcatta attaaattag acggaacagt taagaaattc cgtgtaacga aattatttgg
    59641 tttctttgga ctaaaacgtc tagagattca agaagcaaaa gctggagact tgattgctgt
    59701 ctcaggtatg gaagatatct ttgtcggtga aacagttact cctgtggatc atcaagatgc
    59761 gttacctatc cttcacattg acgaaccaac attgcaaatg actttcttag ttaataattc
    59821 accatttgcc ggtcgagaag gtaaattcgt aaccgctcgt aagattgaag aacgtttgat
    59881 ggcagaactt caaacagatg tatccttacg agttgaacca acaaactctc cagatgcatg
    59941 gacagtttct ggtcgtggag aattgcattt gtctatcttg attgaaaaca tgcgccgtga
    60001 gggatacgaa ttgcaagtat ctcgtccaga agttattgaa aaagaaatcg acggtgtaaa
    60061 atgcgaaccg tttgaacgcg tgcaaatcga tacaccagaa gaatatatgg gtagtgttat
    60121 cgagtccttg agcttacgta aaggtgaaat gcaagatatg gtccatacag gaaacggaca
    60181 aatccgtttg acattcttaa cacctgcccg cggtttgatt ggttattcaa ctgaattcct
    60241 ttcaatgaca cgtggttacg gaatcatgaa ccatactttt gaccaatatc tgccaatgct
    60301 tcctggtcaa attggcggac gtcaccaagg ggcgcttgtt tcgatcgata caggaaaagc
    60361 aacgacttat tctatcatga gtatcgaaga acgcggtaca gtcttcgtag aaccaggtac
    60421 agaagtatat gaaggaatga ttatcgggga aaacagccgt gataatgatt tgactgtcaa
    60481 catcacgaaa gcaaaacaaa tgacaaacgt acggtctgct actaaagacc aaacatctgt
    60541 catcaaaaaa ccaaaacaat tgacgttgga agaatctttg gaattcttga atgatgatga
    60601 atattgcgaa gtaacaccag aaagcattcg cttgagaaaa caaattttag agaaaaatgc
    60661 tcgtgaaaaa gcatctaaaa agaaaaaata atctgtaaaa ggatgaagag agaaaccagc
    60721 ttgttataaa aaatagctgg tttctttttt tgtccaactc ttttctgtgg agaagtcatc
    60781 tttgtcaatg gacataaatt cagaaaatac gccgaaagac gtttaaaaga cttttaaaaa
    60841 acttatttct agagagtttt tgtttcataa taagataatg tttcacgaaa ggagatcaac
    60901 ttgtccttcg aagaaaatcg aagtaaaatg gcaagagaaa ggagcgggaa gatgaacgac
    60961 ttattaaaac aatactttgg ctatgatgag tttagacctg gtcaaaaaga aattatccag
    61021 aaagtcattg atcaggaaaa tgtattagga atcatgccta ccggaagtgg gaaatcaatc
    61081 tgttaccagc ttccagcttt actattagat gggcttactg tggttgtgtc ccctttaatc
    61141 tctttgatga aggatcaggt ggacgcggcg aatcaattag gtatacccgc cacttttatc
    61201 aatagctcgt tagatggtta cgaaacagct agacgcttcc aagaaatcga tagacagcag
    61261 taccgtttac tttatatcgc tcctgaacgt tttatcatgc cggattttat ccaagcaatg
    61321 aagcgatgga acgtccgtat gatcgctatt gacgaggcac attgtatctc tcagtggggg
    61381 catgatttcc gcccaagtta tttacagatg gcgaaccagc tagatcaact gccaaatcgt
    61441 cctgtaattg ttgctttgac agctacagcg actgtgcaag tagctgctga cataaaaaga
    61501 cttttaaaaa ttccagaaaa caaccatatt cagactggtt ttgaacgaga aaatctacga
    61561 tttcaagtaa ttaaagatca aaaaaaagaa caatatctaa tcgaatattt aaagatcaac
    61621 aaaaatcaat ctggtatcat ttatgcagca acgagaaaag aagtagaccg tctctatcat
    61681 ttgcttaaaa aattcgattt ttccgtgggt agatatcatg gtggtctaaa tgagaatgag
    61741 cgaacagaaa tgcaagaagc ctttttatac gatcgcttgc agctgattgt agcgacaaat
    61801 gcatttggta tgggtataaa taaaagcaat gtccgttttg ttatccatta tcaaattcct
    61861 ggctctctag aagcatacta tcaggaagct ggtcgggcgg gaagagatgg tctttccagt
    61921 gaagccatcc tgcttttctc tcctcaagat atccaagtcc aaaagttttt tatccagcag
    61981 tcccaaagag aagaagggca aaagcaaaaa gaatatgaaa aattaaaagc gatgacagaa
    62041 tacgtgtata ttgagtcctg tcttcagcaa tacatcctaa actattttgg cgagacaagc
    62101 agtccgtgca accgttgtgg taactgttta gatgacagag aaatagtaga agtaacgaca
    62161 gaagcacaga tggttctttc ttgcttaaaa cgtatgggtg agaactatgg taaacaaatg
    62221 ctgatgaaag tcttagcagg atcgaaagaa caaaagctgc aagctttagg ttttggacat
    62281 ttatctacct atggactgat gaaaaaccaa tcgcaaaaag aaacgatgca gttaattgaa
    62341 tatttgatat cgaatggcta tcttctgacc atcaatggtg agtatcctgt tttgaaagta
    62401 acagaaagag ggatacaagt attgaaagga caagaatctg tttatcgaaa ggaacccaag
    62461 aaagtccagc agctatcgga tgaagaaacc gatacactat ttgaagtgct gcgggaactg
    62521 cgaacggatc tagcatcaga agcaggcgtt cccccgtatg tcgtattttc agattccaca
    62581 ttgaaagaaa tgagccgaat acgtccatca agcagattgg agatgcttca gataaaagga
    62641 gttggccaaa gcaagcttga taaatatggc gaagcctttt tatctcggat aaaaaatgcg
    62701 gactctaacg ttctggataa gtagaaagaa cgaatacgta aaaatgaata aaaacaactg
    62761 gttaaatctg ttttttttgc tccttatgaa aatcaaatat gttataatta aggaattaga
    62821 atgggaggta agcacagatg gattctttgt cgaaaaaact tgcgatccaa gtattaaatg
    62881 atgaagcaga acggatcaaa cgattaatcc gaaatcaaaa caacagtctc tgtatctcac
    62941 aatgcaaagc cttcgaagaa gtggtagata cacagatgta cggcttttcc cggcaagtcg
    63001 gttttgctgt ccgtatagga gtaatcggaa agaacgaggg ccagctgatg ttaagtgagc
    63061 tagaacgaga attgaatcag ttatatagtg aggtttacca agaaatttat gataaaaaag
    63121 aagttggcaa ggaggaatag acttgcctaa gaaagtgaaa aaaaggcatc tattagacta
    63181 tagcatcttg attccctatt taattttatg tgtattggga ttgatcatgg tatatagttc
    63241 tacgtcgtat ctattattag aaaacggaca aaatccgtcc gcatcagtca ttaaccagag
    63301 tattttttgg gtgcttagct tgattgctat cgcactactt tataaaatga aaacagatgt
    63361 tttgaaaaac cagcgtttga tcatggcagc catcgcagtt ttgacgattt tgctgctgat
    63421 tgttgtgttt tttggtaagg agatcaatgg ggcgaaaggt tggctgcaaa ttgcaggatt
    63481 ctcgatccaa cctgccgaat acctaaaaat catttctatc tggtacctgt ctctgacttt
    63541 atctaaaaga caaaacagtg tacaaaaaga ttttcttggg actgtgaaac gtccacttgc
    63601 aatggttatc ggacttactg cattagtcgc tattcttccc gattttggta atgcggcagt
    63661 tatctttttg attattttgg ttctgctttt agccagcgga gtcaattatg tgtatacttt
    63721 gattgtagga gtaggaggct tttgtctaag tacttttacg atctggctga tcaatatcac
    63781 aaacggaaag atcttacccg gacgtttaca gtatatctat aatcgatttg ctatttacca
    63841 aaatcctttc tcagacgaat taaataaagg gcatcaacta gtcaacggct actatgccat
    63901 gttcaacgga ggactatttg gcaggggatt aggtaacagt atccagaaaa aaggattttt
    63961 acaagaagca caaaccgatt ttatttatgc aatcgtagtg gaagaactgg gagtgattat
    64021 ggggattttg atattagcgt tgctattctt tatgattgtg cgcattgtcc ttgtaggtat
    64081 ccgttcaaaa gatccgttca attcgctttt gtgcattggg atcggcgcaa tgtttttgat
    64141 ccaagtattt gtcaatcttg gaggaattac tggcgttatt cctttgacag ggatcacgtt
    64201 cccattttta agtcaaggag gctccagttt gctgatgctt tcgatttgtg tcggctttgt
    64261 cctcaacatc agtgcggatg aaaaacgcaa gagcttaggt ttataataaa aaaagatgta
    64321 ggagtggttc gatgaaaaaa gttttagttg ctaacagggg tgaaatcgct gtacgtgtat
    64381 ttcgtgcttg tacagaatta ggaattaaaa ctgttggaat ctatgcagaa gaagatgaat
    64441 attccgtaca ccgtttcaaa gcggatgaag catatttagt aggacaagga aaaaaaccga
    64501 ttgatgcgta tctagatatt gaagggatca tttctatcgc gaaagaatgc ggagctgacg
    64561 ctattcatcc gggttacggt cttttgtcgg aaaacttgaa ttttgcgcaa cggtgcgaag
    64621 aagaaggcat catttttgtt ggacccaaac tgcatcattt agatattttt ggagataaaa
    64681 tcaaagcaaa agctgcagct atcgaagcgg ggattgcttc gattccaggc acagatggac
    64741 caatcgcttc tatagacgat gccttggagt ttgcgaaaca atacggttat ccgattatga
    64801 tcaaggcagc tctaggcggc ggcggacgcg gcatgcgtgt agctcacgat gaaaagagtg
    64861 caagagaggg ctacgaacgt gcgaaaagcg aagcaaaagc agcctttggt agtgatgaag
    64921 tgtatgttga aaaatacatc gctaatccta agcatatcga agtacagatt ctcggggata
    64981 cgcatggaaa cgtcatccat ctttttgaaa gagattgttc tgttcagcgc cgtcaccaaa
    65041 aagtagtaga agtagcgcct tgtgtctcta tgaatgaaca gcaacgtcaa aaaatctgcc
    65101 aggcagctgt ccaattgatg aaatatgtcg ggtatgtcaa cgcaggtaca gtagaatttc
    65161 ttgttgaagg agatgatttt tactttatcg aagtcaaccc tcgcgtccaa gtggaacata
    65221 cgatcacaga aatgattaca gatattgata tcgtaacaac gcaactgttg attgctcaag
    65281 gtttggatct gcataaagaa atcggattgc ctcagcaaga aggaattaaa ttaaatggtt
    65341 cagccattca atgccgaatc actacagaag acccgttgaa taacttttta ccagacacag
    65401 ggaaaatcga tacgtatcgt tcacctggtg gttttggcgt acgtctggat gtcggaaatg
    65461 cttacgcggg ctatgtagtg acaccgtatt ttgattcatt gctagtaaaa gtatgtacgc
    65521 atggtgctac ctttgaaaca gccattcaga aaatggaacg ttgcttgaaa gaatttagaa
    65581 tccgtggagt gaaaacaaac attccattta tgctcaatgt catcacacat cctgaattcc
    65641 aatcaggaaa tgcaaaaaca accttcattg atagtacagc aacattattt gaatttcctc
    65701 gactacgaga ccgcggaaac aaaacgatga aatatatcgg tgaaattact gtgaatggtt
    65761 ttccaggtat cgaaagtggc gaaaaaccat tttatgaaga accacgtatg cctaaagatc
    65821 taatcacaag aactgattac gtaacggcta agaatgtatt agatgcgaag ggtgccgatg
    65881 ctttggtcga atggatcaaa ggacaggaaa atttgttgtt gacggatacg acatttagag
    65941 acgcccatca aagtctgcta gcaactcgtg ttcggacaaa ggatttcaag cagattgcac
    66001 gtttgactgg agaaggtctt cctgaactct tttcaagtga gatgtggggg ggagcaacat
    66061 ttgatgtggc ttaccgcttt ttgaatgaag atccttggca acggttgaga aaaattcgtt
    66121 ctttaatgcc taatacgttg ctgcaaatgc ttttcagagg ttcaaatgcc gttgggtatt
    66181 ctaattatcc tgacaatgta ttggtggaat ttgtcaaaga agcagcagcg caaggaatcg
    66241 atgtatttcg aatctttgat agtttgaact ggactccaca aatggaaaaa agtatccaag
    66301 ctgtacggga tactggtaaa atcgcagagg cagctatttg ctatactggc gatatcaacg
    66361 atccaagtcg ggcaaaatac aatgttcagt attataaaga catggcaaag gaactagaac
    66421 aactgggtgc gcatatcatt gcaatcaaag atatggctgg gctgcttaaa ccgcaggcag
    66481 cttatcgttt gattagtgaa ttgaaagaaa cgacagatct gccaatccat ctgcatactc
    66541 atgataccag cgggaatgga atcatcactt attctgctgc ttcaaaagca ggagtagaca
    66601 tcgttgacgt ggcaatgagt gcaatgagtg ggaatactag tcagcctagc atgagcagct
    66661 tgtattatgc actggtcaat ggtccacggc tgccagaaat cacgattgaa aatgcacaga
    66721 aattaaacca ttattgggaa gatgtgcgca tgtattacaa accgtttgaa aacggcttga
    66781 atgctcctga aacagaagta tatatgcatg aaatgccagg aggacagtat tcgaatctac
    66841 agcaacaggc aaaagctgtc ggattaggtc atcgctggga tgaaatcaaa caaatgtatc
    66901 atactgtcaa cttgatgttt ggcgatatcg ttaaagtaac tccatcatct aaagtagttg
    66961 gagatatggc actatttatg gtccaaaatg atcttacaga agaagatatt tatgaaaaag
    67021 gcgaaacact aagtttccct gaatctgttg tgactttctt ccaaggagaa ctagggcaac
    67081 ccgttggcgg atttcctaaa aaattgcaaa agattattct taaaggcaga cctgcattga
    67141 atgaacgtcc aggtcttttg gctgaatcag tcgacttcaa cgaagtaaaa aaagaattgg
    67201 cagaaaaaat tggctatgaa ccaaaacaag acgaagtatt aagctatttg atgtatccgc
    67261 aagtcttttt ggactatcaa aaagcatata atcaatttgg cgatgttaca ttactggaca
    67321 caccaacatt tttccaaggt atacgtttgg gtgaaacaat caatgtacaa atcgaacgcg
    67381 gaaaaacgct tatcattcgt ttagatgaaa tcggagaacc cgatatcgaa gggaatcgcg
    67441 tactgttctt caatttgaat ggacaaagac gtgaaatcac tatcaaagat aattcgatca
    67501 tcagtacggt ccaaacccga ttgaaagctg aaccgacaaa ccgagaacaa attggagcaa
    67561 cgatgtctgg ttctgttttg gatgtattag tgaaaaaagg cgataacgtt aaaaaaggtg
    67621 atacgttgat gattactgaa gcgatgaaaa tggaaacggc catcgaagca cgttttgacg
    67681 gagaagtcgc acatgtatat gtgtcttcag gagacactat ttcgtctggt gaccttttga
    67741 ttgaagtgac tgagaaataa taagcgaaag gtggggaaac atgaaaagac tgggtttgtt
    67801 ttggctgatt ctgctattag ttattgtggc attctacatg gaaccagtct tgtttccgcc
    67861 aaaacttgaa gtgaaggaaa actctgatca acaaatatca aaccaaatga caacgtggaa
    67921 ataccaggag cttaaagcaa ctggtttttc tacttatatc ggaaaaccga tcgatcagct
    67981 ggaaaaggat tttggccaag cctatgatcg attaactagc ggatttggtt ttgaaacaag
    68041 atattatgaa aataaagcta gtcatctcac atttgaagcg aacatccaag acagcagagt
    68101 cacaactgtt aaagtactgc aaacagggcc tgctgatatc gcgccgtttt atttagggat
    68161 gacgatgcaa gatcttacga aaatcactac tatctatact aatttcactt ttgagtacca
    68221 tgatacagaa gttggaatcg aactaatgga agaggatatg aattatcgtc cgctaattgc
    68281 ttttgataac gacacttttg ctattttatt ttttgatcaa acaacaaacg gcctattttc
    68341 agttgtgtat ttagataaag atagtctttt gaaactattg ccatatcaag tgtttggtga
    68401 aggccttcca cattatcaag cagataaatc ggctgattgg gaatcaatca atcgcgttaa
    68461 agaacgaaaa agtacgactt tattaaatat gcttagaaga gaagacgaac ttcccatata
    68521 caatcaaaca cttgcctttc aaaaccacag tcagctgcta ctacatgatt ttttgacaag
    68581 gcctgaggaa atattgtcag aggaacgtat ggctgattgg caaaaatcgc taggcagtgc
    68641 tcaaacgaca gtgaaattct cactgacgaa tgatgaattg gagaagttaa tcagtaaaca
    68701 aaaattagaa aatactagtg ggctatttat tcatccggcg attgacgcta ctttttcgtt
    68761 tttatattgg tacagcgatc cttacaacca tgaccgtttc atggacccgg gaacggattc
    68821 tgtaggcatt gctttttcta aagaaaatat gttagtctta ttgcaagaag aaaacggaga
    68881 aagtagtgat agcagtgatc gttaacgaag agctgtttca actggaggat caatgcctcc
    68941 aagtcgctga aatgataaaa aagagtaaaa cgatggcttc ttatcttgaa cataaacagt
    69001 caatggaaga atctgcaaaa gtttgccagt tgaaagaagc gttcttgtct aagaaagaag
    69061 catttgaaaa tgtagcggca tatggtgagt atgcacctga ttatcgtgaa aaaaaacgag
    69121 ctgttcgaaa agcaaaaaga cagcttgata tggatgagca tgttgcagct ttccgatata
    69181 gtgaaacaca gctgcaaagt ctgttggatc aaattgtcca agtacttgcc gaagaagttt
    69241 ctaatgaaat caaagtagat gcaggtagcc cgttttttga aacgaaaaaa cactttggat
    69301 gcggaggtaa ttgtcatgca attagacaat agtgaatttg aaataacaaa acgacgcggg
    69361 ctgatcgtat gggtctacag cttaaaacaa ttaaagaatc tgaaaaagtt cggcttgttg
    69421 cattatgtct caagaagaat gaaatatgtc gtgctttact taaatgaaga gacattggaa
    69481 gaaacagaag aacgaatccg taaattacat tttgttcgaa acgtagaacg ttcttaccga
    69541 ccggatatcg aaatgaattt tgccgaaaaa ataggaaaca aaacagtatt aaaagacgaa
    69601 ggtttcgaaa ttgaagagct aaatacagaa atccgattag ctgaacatct ttaaaaatct
    69661 cttatgagca gtttttaaat aaaaaataac ttggataacg atcgattttt gatcattgtc
    69721 caagtatttt tttggtatac tagtaccagt accatttcta aggagtgagt agatgcggat
    69781 aatatcagga aattatggcg ggcgccgttt aaaaagttta gcaggggcga acaccagacc
    69841 aactacagat aaagtaaaag aatccatatt caatatgatt ggaccatatt ttgatggaga
    69901 gacagtattg gatctgtttg caggaagcgg tggtttagct atcgaagcag tatctagagg
    69961 ctgcagtcat gcggtatgcg tagacaaaaa ttaccaagcg ctaaaaatca tcaaagaaaa
    70021 catcgaaatt acgaaagaac cagagaaatt taccacaata aaacttgatg cggacaaagc
    70081 aatcgaacgg ctagcagcgg agaaacaaca attcgactac ttgtttcttg atccgccata
    70141 tgcaaaacaa aaaataatcg atcaaatcca taaaatggaa gaactgggac ttttttctaa
    70201 aaccgcagtt atcgtatgcg aaacggataa aacagtcgaa ttgccagaaa ctgtggctgg
    70261 attccatcaa ataagaaaac agatttatgg cataacggcc gtaactatct atagaaaaga
    70321 gggaaatcaa tgaaacgtgc attattccca ggaagttttg atccttttac aaaaggtcac
    70381 ttagatacgg tggaacgtgc ggctaaatta ttcgatgagg tagtaattgg tgtttttatc
    70441 aatacttcaa agaaaagttt gtttccccca gaagaaagaa tgacattgat cacaaaagct
    70501 gtttctcatc ttcctaatgt aaaagtcatg catcaagaaa atcaattgac ggtagaaaca
    70561 gcaaaagaga ttggtgcaga tgcgttgatc agaggcatac gaagtatcaa agactttgaa
    70621 tatgaacgag agattgctca gatgaatcat catctatatg atgaattgga aactgtcttc
    70681 ttattagcaa aaccagaata tagtcacgtg agttccagta ttttaaaaga agtactgcac
    70741 tttggcggag atgtgagcag ctacttaccg ccagtgatca acgaagcttt agctggaaag
    70801 cgtgaatcaa atgaaagcta aacaatggtt gaaaacagta ctgattatca tatttggaat
    70861 gttgatcatt ggatttgtag tagttccctt gccgtattat gtagaatcac cgggagccac
    70921 gatcgatctg aaagaactaa ttacggtcaa tgataaggaa gataaaggtt ctgggtcttt
    70981 ctctcttacc tctgttggta tcaggcaagc tacaggtttc ttagctttaa aagctaaaat
    71041 gacccctttt gaagaattga tcagtgaaga agaattgact ggtggggcga caaatgaaga
    71101 atataatcaa atgcaacagt attatatgga ttcttcccaa aatgcagcaa tcgaacaagc
    71161 gttaaaactt gcagacaagc cttataaaat ggaattcaaa ggcgtatatg tcatgagtgt
    71221 agagaaaaca tcgaatttct ttggcaagct ttcagttgga gatacagtaa caaaagtaga
    71281 cgggaaaagc tttcaaagta cagaagcatt tatggattat gtaaaaagcc aaaaagttgg
    71341 acaaacaatc gaaatcagtt atttgcataa tggtgaagaa aaaacagctt ctggtgatct
    71401 gatcgagtta ccaacagata aaaaggcagg aataggtatc acgcttactg accataccga
    71461 gatcgaatca gatacagatg ttcgtattaa ttctggctct atcggtgggc catcagcggg
    71521 attgatgttc actttagaaa tctatgaaca agtcactggt aaaaacttgc gacatggtaa
    71581 gcaaattgca ggaactggaa cgatcaacag tgaaggagaa gttggccgaa tcggaggaat
    71641 cgataaaaaa gtggcaagtg cggataaagc tggcgtagag attttctttg caccagatga
    71701 tacgatttca gacgatgtta agaaagaata tcctgaaata aaaaccaact acgaagaagc
    71761 aaaagctgcc gctaaaaaat tgaattcctc aatgaaaatc gtaccagtaa aaacagtaca
    71821 agaagcgctt gactatctag agaaaatgaa atgaaactaa ataattaaag aggggttgtg
    71881 acacgagttc tgtcacaacc cctctttttc gtcaaaaatc ttcacgataa aaataaaaac
    71941 aaaagaaaaa attgacgtaa ttagatgttg aagggacgat tttgccagag ctcactgttt
    72001 cttcaatttt aaatgaaagg agtaatgaat gatttttact ggtcattctt tgaaaaaaga
    72061 acaatgatag aaaaaatcag caagaaaata ctagcttcac ccaaattgat catttggatg
    72121 tctatgatag ttcttatctt ttgtatcgca agtggttttt tattatcaaa tttgctattt
    72181 tatagagaaa cagaagagac tgctgcaaaa gaaatacttt cttcgtttag ttccagtgga
    72241 gaagatccat cagctgaagg aaacgatgta gaagaggatg ctcgtccaaa ggtcatgtac
    72301 accgatatca aaggaagtgt gaaggagcct ggtatctatt ccttttcttc agaagaacgg
    72361 gtctatgatg tactaaagcg tgcaggtgga ttattagaag aagcggatag cgatcgaatc
    72421 aacttttctg cgaagataga agatcagcaa gtcctatata tccctgctgt aggtgaagag
    72481 ctgccagaac atcttgatca gttggcatcc tcggaaggaa aacagcctac agcagattcc
    72541 gagcctccca aaataaatat caatacagcc actccatcag aacttcagca gattcctggg
    72601 atcggatcag tcaaagcgca agaaatcatt cgatttagag aagaaaacgg atccttccag
    72661 aaagtggaag acctgcagga gatttctggt atcggtgaaa agacagttga aaaactaaaa
    72721 aacttcgtta caattaaata gaatgattca aaatcgactg gcacgagcaa taaatggacg
    72781 ctaatggata ctaagaataa tccaattcta agagcttggc aggacgagga ggtagtattt
    72841 ttggatagga aatagcttga ttgttcgcaa agaaacatta aaaaaaccaa tgctgaatgt
    72901 ctatctccaa aataaaatat ctgggattca tattatgaat acggctgtca gcggcaataa
    72961 cagtcaagct ctacgagaac gctttgccaa agatgtactt agctatacag cagataaagt
    73021 atttattctc attgggacga atgatctagc agagcataaa caactttcta aagaaacgta
    73081 ccaaaaaatc tgcagtggat gatagttcag ctgaaaaaag actatagttc tgatcatgct
    73141 ttctttatca cgccaccagc agtcgatgag acaaaacaaa aatatcggaa taacaagttg
    73201 atcaaagaat atatctctgt tctcacagaa gtagcaaaac gccatgattg tgaagtatcg
    73261 atttttatac ggaatttctt gaacaaatgg attcgaagaa tttttataat gggctaatgg
    73321 atgacggact tcatttcggc agtgaagggt aggatctttt gtcagacagg atgattcgtt
    73381 atatttaaag aatattttgt ttaagcaatg agtacttttt aatcaacttg aaaaaacgga
    73441 ggaatataaa acatgttaga aaaatcttca aaagcatgcg atacgaataa agaaaaagtt
    73501 actgataagt cacataatga acgcattcct tgggaccagt actttatggc tcaggcagta
    73561 ctgttatctt tacgtagtac gtgtacacgt ttagaagtag gagcgacact cgttcgagaa
    73621 aaaaggatca ttgctggcgg ctataatgga gcagtcagtg gagatgtcca ctgcatagat
    73681 gaagggtgtt acgtcgtgga tggacactgc ttgcgaacga tccatgcaga gatgaatgca
    73741 ctcctacagt gtgcaaaact tggtatcccg acggaaggtt cagaaatcta tgtcacacat
    73801 tttccatgtt tggcctgtac aaaggctttg ttgcaagctg gtgtgaaagg gatcaattat
    73861 ctccatgact atcgaaatga tccttatgcc caagcgttga tcgaacaatt aggtgtatct
    73921 gtacaccaag ttgctttaga ctcacagcat ttttcaaaac ttcaaaatga attaacagga
    73981 aaacatgtct agcgttttag aaaggcatgt tcgaaatcgg ttgatttttc cggcaattac
    74041 gtcgatgatt atctgtttgt tgtattacac tgacaattgg ggttatcgtt tgcttcttgc
    74101 cggaattttt ttatggtttt cgctaaaaga aggacgtttt atcgcgtact tgtgtatttt
    74161 gtgtagcata acggtatcca tttcttatag gctacaagag tattcatttt tagaggaaaa
    74221 aacaacatct gcgcaaatta ctgtatttac agatacgatt cgtgcaaatg gtaatttttt
    74281 gacaatggaa ggaaggttat cagagaataa acaaaagctc cagctttctt atcaagtaaa
    74341 cacagaagca gaattgaaaa catggcttaa cttccgagga aagggaatag aattgactgc
    74401 tgaaggcgag ttcatctctc cagcaaaaaa gcggaatctt tataattttg accaagaagc
    74461 ttacttcagg aaccatagaa tttctggacg gttccaaatt caaaagattg aacagatcac
    74521 catgcaaaat cactggcgaa actggcttca gagaaaaaga ggatctttta tctcctttgt
    74581 ccaatctgaa tttccgcaaa aaacaagtgt atatattaat tctttattat ggggctataa
    74641 agatgcagga ttcagagaac cagaagaaat ttttagggag agtggtttgc tgcatctgtt
    74701 cagtctatcg ggtctacaca tccaatttta tttagggtgg ctgtattatt tatttcgacg
    74761 tatcggatgg ccactacacg tcagtattat tccgttgagt ctactaacta tctgttatgt
    74821 ttctattgca ggaagttcta tttctgtttt gcgtgctagt ttgctgtttt tattgcgatt
    74881 gctagtcaaa ttgctgaata agcattattc caccatggac ttatttgcag ttgtcttttg
    74941 gcttctttta ctatttgatc ctcgatttct ttttcatgcg ggtggacagc ttagtttttt
    75001 catgagtttt ttgtttatcc tgatttcttt tgatggagat tggttgaaaa aaacggcctc
    75061 tcacgtcctg tttactatga tcacgatgcc gttgattgta tggcattttt atgagtggcc
    75121 tattttaggt agtcttttta cattactgat tgcaccggta ttcaagtttc tttttcttcc
    75181 tggtgtgtta tttctcacac tcttcaatcg ctggctgccg gaaactttac ttttgttgat
    75241 agaagaggga ttaatcttat ttgagcgaat aatcgatttt tcttcagggt cgtctctcat
    75301 tataggacgt ttgccccttg tgctatctgg gatgtatatt ttcggattac tgatttgtat
    75361 ggataggttg aaagaacaac ctgtgaaaca cttgttttat gttggtcttt tttcttttat
    75421 ttttttgact ttgccatttt tacgcttttc ttcaacgatc gcttttatcg atgtcggtca
    75481 aggagacagt atctttttgc aaagtccttt tcgaaaagaa acgattttga tcgatactgg
    75541 aggtcagtta cactttgaaa gagaaaagtg ggcgcaaggt atgatacgtc ctcctgctga
    75601 aagaaatatc gttccatatc taaaaggtca ggggataagt gtaatcgata aactcatctt
    75661 gacacacgac gatacggatc atgtgggaga attacccacg ttatctcagc attttactat
    75721 caaaaaaatt tatatcggtt ggggcgctgg acgaaatgag ccattacaaa ggcatttatc
    75781 tgaagctcaa aaaaatggta ctgagatcat tgaaatcaaa catggggaca ggatcagcgg
    75841 ttattatgat ctttatgtat tgactccttt tgaaaaggga gagggaagaa atgaagattc
    75901 gattggtcta tggatggaat ataacaatag gcgcttttta tttttaggag atttgaatca
    75961 agcaatggaa aaacaattgc ttttgattta tcctaatctg aaagccgatg tagtcaaatt
    76021 aggtcatcac ggtagccgta cgtcaagtaa cacagatttt ctttcccata ttgaagcaaa
    76081 acatgggatt atatcctgcg gcgtaaataa tcgttatggt cacccgcaca gcgaagtatt
    76141 ggagacacta gaagaaaatc agataaaaac atggcgaacg gatcaacaag gaatgatcac
    76201 ctatcgctgg catccagttt ttcatcctca tgggctagtg gaaacaatga tagactagta
    76261 attctcatct gctcatggta ggataatttt tgaaagtggt gagtaatttg agtatacaag
    76321 cagagttaca aaaaatacgt aaagaaaaac ttgcatcttg ttacttgatt ttaggaacag
    76381 aaaagtttct ccaagatcaa gttcggacag aaattgtcaa aaaaatccaa ttagagggcg
    76441 aagatgactt aaatttttta agttttgaca tggaaaatgc aagtttagat gaggtagtag
    76501 ctgaagcaga gacgttgccg tttttcggag atcaacggtt agtattcgta gagaatcctt
    76561 attttttaac aggtgaaaaa gtaaacaatg gaatcgaaca aaatactgat ttattgacgg
    76621 cttatttgaa agaaccatta gaatcgactg tattagtatt ctttgcgcca tatgaaaagt
    76681 tggatgaacg gaaaaaagta acgaaacaat tgaaaaaaac agcaattatc atcaatgtcc
    76741 aacagttgaa tgaaaaagaa gtacgtcaat atctgatgaa taccttagag aattcctcaa
    76801 tcaaaatgga acgaagtgca attgatttat tcttgaggtt gaccgactta gatttatcca
    76861 aaatgatgag agagttacag aaattggtcc tttatggaca aaatcaacag caaatcacgg
    76921 taagagaagt agaagaatta gttcctaaaa cgttagaaca taatattttt gacatgaccc
    76981 aatatatttt aaaagggaag acggaacaag ctttgcgttt atacgaagat ttagttatgc
    77041 aaggggaaga aacaatcaaa attaatgcga ttttgctttc tcaattacgt ttatttttac
    77101 agacaaaatt tcttgtaaag attggttatc agcaggcaaa tattgctgaa acattgaaaa
    77161 tccatccata tcgtgtaaaa cttgccatgc aggaagtaaa aaaattcgat gaacatttgc
    77221 tggtacagtt gtttgatcaa ttagttgaga tggattatca aatcaaaacg ggacaaatcg
    77281 aaaaagaatt aagttttcaa ctattcgttt tgcgcacagg gcagttgtta agaaatcgat
    77341 aagaagaaag acgagggcaa agcatgcggg aaggtcgttt gtttttgtcc tttttttgac
    77401 aaataaaaaa atcggccgaa gccgattctt ttatttagcg atttttttgc ttaaacgaga
    77461 tttgtcgcgg ttagctttgt ttttgtggat tagacctttt gattcagcca tatcaatagc
    77521 tttaacagct tctttatata acgcatctac gttgtcagca cctgaagcta cagcatcttc
    77581 aaattttttg attgcagtac gcatagcgct tgtttgagat gaatttttca cattagcgtt
    77641 ttcgcttgta cgaacacgtt tgattgcaga ttcaatgttt ggcattagtt tcacctccga
    77701 taacttaagt tcttacgcgt gttaccgcat aattcaacat tcattattat acctaaaggc
    77761 agcgtgcttt gcaatatgaa catgacaagt ttttttcctt aacagtcttt tattaggatt
    77821 ttttttcagt aaaaacggat agtacaagct gaaaaaaatc aaaaagacgc tattgtggga
    77881 ggcaatagcg tcaaaccaaa ctagattttt atttatctat ttaaacaaag tttgaatggg
    77941 aatgtttggt actaagttat tatataacaa agagaaccgt ttgcaaagtt ttttttgaaa
    78001 agggatacga ttccctgttg ttctttgttt ttctattaag aaactgcagt tattttataa
    78061 ccagtatcct ttgatcagta aaggctattc tggtgtaccg attattttaa tgtattcatc
    78121 gatatttttt atcaacattt tttttatgac caactggttc cgacggttcc atttgccact
    78181 tacttccagc acatatttat ttaggggcaa acgtaagata tattttgcca aatagcgatc
    78241 tttaagcagg ccattgatca cacggtcctc aagctgcaag ttgatcccag tcagcatcat
    78301 tggctgttct tgtaaaacgt acgtatcaat taaaagacct tcaaaagttt ccacaagcgt
    78361 cttcctttct tttcaaagta acctgaaaac aggcactaat tcttccattt tgatattcgt
    78421 tgaagtggat gcgatcattc tttcttttgt ccactgtaat ctagtataac aaaaaagagc
    78481 agaagagcca aaaagaaaaa agagcatcga attctatagg aagtctcatt ggcacggcct
    78541 aaattattac aaaacattga ttaaaaaaag cccctgcatc gcaggagcat cccctatatc
    78601 gttttaatag gtcaatcaaa aagcagtatc aaacacattt tgtatcaaat caggaaataa
    78661 tagataagct aagcccagaa tcaaaagtac cgctagaaga aaccagccga ttgttcctat
    78721 gaaatacttt aatttttcga acattgtatc acctcgttgt cagtatatca cggatcagta
    78781 aattgggggt gttaagaatc tcctaaaata gacaaaattt gttctatttt cctaaagcaa
    78841 atacttgtaa gatacagtaa gtgataccag aagcaatgac cggtccggcg gcgattcctc
    78901 ttaagaagac aacacctaaa atcgtgccga aaacaagtgc aacagtgatt tgtggatcag
    78961 aagcaatcaa tcctacaccc ttggaagata agacagccac tagcccaccg caaaaaacag
    79021 ctacccagcc aataggggat ttaaaggctt ctaatagatc acgtagaccg atttctcctg
    79081 ttgcgatagg gattagaata gccgctgtga tgatcgtcac cccaatattg attcctttgc
    79141 cttgaagcca agggaaaaag gaacgggagg ctggtatgag cttcataatc aaaacaaaag
    79201 ctgccgctat catcagactt tgatttttag ctaaaattgc tacaagtaaa ataattccta
    79261 aaaaaagcca actttccatg aaatatattc ctcactttct tcgaaacaat cataacatgt
    79321 ttttagaaag ttacaaaagt aggaattcat ccttcaagac ctattcataa gctgtttttt
    79381 tcataaaata aacaaaatca taaaaaaaca ctttgcttat ttgacagaag acagccgcgc
    79441 tttgtatagt ggacaagact tacatttttt tgaagaatca gaaagatcag gtaaaggtct
    79501 tagtaaacgc aagccattca gaataaccaa tattgtgctt ccttcgtgtc ctacaactcc
    79561 aagaggtaga tttaaaaatt gcaggaagtt agaagcaatc aacaagagaa taacaagaat
    79621 cgaaaaaata atattttgtt tgatgatccg ccgcattttt aaagatagcc gatgactgta
    79681 gacaaattta tttaagtcgc ttttcattaa gacgatgtct gcgatatcca tagcggcatc
    79741 tgtcccttgc cccatagcga cacctaaaga agcggctgct aaagcaggcg catcattgac
    79801 tccatcacca accatcgcat ttacttggta aagttcattc tcttttttga ctagttgtgt
    79861 tttttcttcc ggagtacagc ttgcatagta atgttcaatc cctacttgtt cagcaatggc
    79921 ctttgctgtg ccagaatggt cgccggttat catacttgta tggatgtttt gggatttaaa
    79981 gtaggaaatt acttgttggg tatttgcttt tggaatatct agcagaccta aaacagcaat
    80041 taactgctga tctttagaaa gataaatcac ggttttgcct tgattttcca aacgttcgat
    80101 catttccgca atctctttgc tgattatcat cgtctcagga tcatatgcgt gcttgccaac
    80161 tttccattgc gcacctaaat aggtagtttg taatccaaaa ccagtaattt cttctattgg
    80221 taagtattgg atttcttcag gtattgtgca atcgaaatat tgcaaaatcg catgagcaag
    80281 tggatgcgtc gttttttgtt ccatagcaac aagaagttgt tgtgccaaat ccttttcatc
    80341 aaagaaaaag ctgtcagtta caactggttt tcctttagtg agcgttcctg ttttatcaaa
    80401 ggcaatcgct tttaattcag aaagcttttc taaatggata ccgcctttga ttaaaatccc
    80461 attttttgca gaatttgaaa gagcagctag agaagctggt gtagcagatg cgacaagcgc
    80521 acaaggagaa gccacaacta ataataccat tcctcgataa aagctttcat cccaactcca
    80581 acctaggaaa aagtggggga gaaggatcat tagaggaaca gcaagcaaaa tcaattttac
    80641 ataattattt tcgattcgtt cgataaatga agctgtttga gttggcgtat tctgcgcttc
    80701 ttctactaat tggataatct tagcaaagac agtttcggaa ctcgtttttg ttactcgaat
    80761 cgtgatggga tttccttgat tcagcgttcc tgcatacatc tcatcgccta aatatttttc
    80821 tacaggcaca gattcaccgc ttaatgcaga ctcatcgatc gttgcctgtg tgctttctaa
    80881 aaagccatct atcggaacag ctgctccttt agcgacaaat accaagttat ccacctttag
    80941 ttgagaaaca tctacttctt gcatttgtcc attttctttt aataattggg ctttttgtgg
    81001 ttgaagattc atcaaagcag taatttcttt ttgactttta ttagttgtgt attcctctaa
    81061 tgcaccgctt aaacaaaaga tgaatgtcaa cattgctcct tcaaagtaat taccgatcaa
    81121 acaggcacca atagcagcta aagccatcaa taaatccaca ttcagtgtcc gatcattcca
    81181 caattctttc aagccttccg atgtttgttt atatccaccg atgataatgg cactgatata
    81241 aaaaagtggg gtgatggtaa gagagaatcg gtcgaagata aaaccgaaga tcataaataa
    81301 aaggcagccg attgttgctg caaaagcttt tttctgatta gtcatcgtta ttagacctcc
    81361 ttgtcaaaac aaggatagca taataaaaat gtaattgata ataataacag ctaaatgaga
    81421 acgataatgg ttatcatttg gtgattaagc ttgatgttgt ttttattatt tataatgatt
    81481 attattaaag aaaataaaaa accagaacat ttctcaaata agagaagtgt tctggttttt
    81541 tctacattgc aataaaccaa taaccaatca aaatgatacc aaggataata cgataccagc
    81601 caaaagcggt aaagtcgttt ttcttcaaat agcttagtaa gaatttgata gctaagacgg
    81661 aaaccacaaa agatacgaat gttcctacaa gaagaacaac agtttcttcc attttgaatg
    81721 aattcccttc agctaaatat ttgacgatct tcaagccgct tgcgccaaac atgacaggaa
    81781 tccctaagaa aaatgaaaac tctgtagcga caaaacgcga agcaccaatc aagatagatc
    81841 ctaggatcgt tgcgccagaa cgggatgttc cgggaacaag tgccaagact tggaataacc
    81901 caacaagaag agctgcttgg tacgtaaagt cattcagaga agtccaacga ggtgttttgt
    81961 ttttgttgcg tttttcgaca ataataaagg caataccgta aacgatcaaa acaatggcaa
    82021 ctggtaagaa cttgtggaaa tttgcttcta accaatcatc caaaggcaaa ccaatgatga
    82081 tggctggtat gcaagcaacg atcactttaa accaaagtgt ccacgtatct tttttttcta
    82141 ccgttgattt acttggagaa aatgggttta acttgtgaaa ataaagaacg acgacagcca
    82201 agatcgcccc taattggata acgacattga acatcgacat aaattgtgaa ctggcatcca
    82261 gtttgatgaa ttcatctgct aggatcaaat gaccagtact actgatgggc agccattccg
    82321 ttattccttc aataataccg agaatcactg ctttgataat atttgcaaaa aacataaatt
    82381 ccttccttcc caattaagat acatcttata gtataccgtt attttttgag aaacccaatc
    82441 tgttttttaa tattaagaaa aacttgagaa ataaagcaga taagtacacg aagagaaaaa
    82501 agaagacaaa gccctcgttc agactttgcc ttttatgatt ctctggtaaa aattaaaata
    82561 tccccattat agaaacgagt cgcgactatg tgttttcccg ttatcgatat aagacgtatc
    82621 attgatgccg tggatagaaa atcgaccgtt tgcataagtg acctttgtta cgctgccatt
    82681 taaaagccca gcacgaacag gctgtttagg atcgatcaga ttaagcaaaa aggcgatcgt
    82741 caaaccatga gatacgacta aaacatttcc accaccattt ttttcgcagt gatgggcgat
    82801 atcttcaaat cctgtccata cacggtctcg caatgtttca taagtttccg cccacccagc
    82861 ggtatctaag ttgtatacag cattagcaat ttgttcaaaa gaaacttctg tggtaaacat
    82921 ttcatcatat gttttaaaat tcaatacacg agggatcaca ccccacattt ccatgtcata
    82981 tccaccttca agagacccga aacaccattc tctaatcctt ttttcaataa aataaggaat
    83041 ttcttttccc ttagaatgtt ctcctagaat aatacgggca gtttcgattg cacgaccact
    83101 gtcactagaa taagcaaatg aaaagtcgac attttttaac ccgcgaccta aataatgaat
    83161 gccttcttgt ccttgctttg tgaggggtgt atcacaccag ccttgtactc gttcaattgt
    83221 attgaacatc gtttttccgt gtcgtacgat ataaagtgta gtttctgtca ttttattctc
    83281 ctcctaagtc gttttaacga ttgaaaaaag gatttgtcct tttttcctgt tcaatcgtcg
    83341 ttgcatcgcc atgtcctgga tagactgcaa attcatcagg taatacaaac agctgggtcc
    83401 gaatactatg aagtaattgt tctaaattcc ctgtatacag atcggttcta ccgatacttc
    83461 ctctaaacag ggcgtcgcca gaaacgacaa aatcatcaaa aataaagctg acgcttccta
    83521 tagaatgccc tggggtaggg acgacgcgaa aagacatgtt tcctaaagta taagttttca
    83581 tttcaaattc atattctgca ggcgagacaa taacatcagc aatatcatca tgtctaccga
    83641 gtccagaaag attcaaaatc ggatcaccta gccatttttg ctctagtggg ctgacataaa
    83701 ctgggatttg ataatacgcg cgtaaaggtt ccactgcacc gatatgatcg tagtgagtat
    83761 gggtaagcaa aatcgctacc ggctgttggt ttgttttttt gatttcttgt ttgattcgtt
    83821 ccgcatcttc tccagggtca atgatcaaaa gtgcttcttc gttgtagacc aaataacagt
    83881 tttcttcaat cattcctgtt tttaatcttt cgatatgcat gtgcattcct ccttgatagc
    83941 caaaacttct ttcatccatt agtataacgc attcattgct aggaaacaaa aacaggtact
    84001 tatttctctt tacttatctg aattatcaga ctatatgaaa agtatttgga tattctaaag
    84061 agaaggaaag taagttttca tagcatttta atatgtcctt gttacattag attcatccaa
    84121 aaatccaaat tctatatcct atatccctgc ctaatcagca gggtgttttt ttatgtttgg
    84181 aaagagagct ctttttgcta aactaggttt tttcaggtaa tcttagagag aaagaggtgg
    84241 tggaaatgat caaacagtca aaaatatctg tgcggcactt agtagaattt attttgcgac
    84301 gtggaagtat cgacaatcgt aaaaaaagta accatactgc attagaagga gcgagaatcc
    84361 accgcaagct ccaaaaagat gcgggagaaa actatgaaaa agaagtcttt ttaaaaacaa
    84421 cagttcaatt agatgaatat tacctgatcg ttgaaggccg tgcagacggt attttcaaaa
    84481 gagacggtat ttattatata gatgaaatca aaacctccga accacgattt gaagatttag
    84541 aaccagaaca agtggaactt ttctttcatc aagcaagggt gtatgcctat atctatagtc
    84601 aagaacatga tttacaagaa ataaatctgc aattgactta cttccagacg gcagaagaag
    84661 tgatcacgag aaaggttgaa catcagacaa gagagcagtt agaaactttt tttaaaaagc
    84721 tgactgaaga ttataaagaa tggcttgttt ttcaagaaaa ctggcgcact gtacgcaatg
    84781 cctctttgat ggctttgaaa ttcccccatg atgagtatag gaaaggtcag cgtgaactag
    84841 cggtagctgc ttataaaacg atccgtacga aacagaaatt gtttgtagaa gcacctaccg
    84901 gaacaggaaa aacaatctct acgttattcc ccgctttgaa agctgtaggg gaagaagagg
    84961 gagaaaagat cttttatcta acggctaaaa caataactag acaagtagct gaagatgcta
    85021 tgacagcatt aaaagataca ggagctgaag taaaaagtgt cactttgacg gcgaaagaca
    85081 agatctgctt tttagatgag acgacctgta atccagatca gtgcccatat gccaatggtt
    85141 attacaatcg gattaatgaa ggtttatggg atcttttgaa tcatgagaat cagatcactc
    85201 gtgaagtcat cgaaacttat gcaagaaagc atacactttg tcctttcgaa ctatcattag
    85261 atgtcagtat ctggtgtgac gtaatcattg gggattacaa ttatttattt gatccaactg
    85321 tttatttaag acgttttttt gaagatgaaa aaaacgagga ctatctgttt ttgattgacg
    85381 aagcacataa tttggtgaat cgttcccgag agatgtattc ggcagaacta tcttatgaaa
    85441 aaacgaaacg aagtaaggaa gctataccaa aggaattcaa aaaacttcat cgccgtttca
    85501 acaaactatt aaaagaattt gattcgatcc gagaaatagc aaaagaagat cattgggatt
    85561 atcatcatca aaaagcaccg gctgaatctt tagtaaaggc aggttatcaa ttaagcgaaa
    85621 aaattaaaga atggctggca gaatttcctg agcacccatc acaagaacaa ttgcttcctt
    85681 attattttga tttgcttcat tttttgaagg tcagtgaatt ttatgatgat cactacgaaa
    85741 caacagtcga aaaaacgtat cgcgatctga tagtaaaaga gttttgtatt gatccaagct
    85801 tgtttcttga gcagtcgttg gataaaggaa aaagcagtat tcttttttct gctagttttt
    85861 caccactctc ttattatcag gaaacactgg ggggacaaaa aagtctggct tacaaactgc
    85921 cgagtccttt cccagaagga aaccagcaag ttttcatcgc aaattaccta gagacgactt
    85981 atcgaaaaag ggaacacagt ctggcaaaat tagtagagac gattcatgct tttgctgatg
    86041 gaaaggtcgg gaattatttc gttttctttc cttcttatca atatttagat cttgcggtgg
    86101 aagcattcag acagcaatat cccgacagca atgtgctaat ccaagaaacg gacatgaatg
    86161 aggaagaacg cgaacgattt ttaacaaaat ttcaaacaaa tcccgctgaa acattgattg
    86221 gattttgcgt acttggcggg attttttcag aaggaatcga tttgagaggg gaccgattga
    86281 tcggcagtat gatcgtaggt gtaggtttgc cgcagatgaa tcatgagcag gaactgatca
    86341 aatcatatta tgatgagaag gaacaccttg gatttgctta cgcttatcag cttcctggaa
    86401 tgaacaaagt cttgcaagct ggtggtcgag tgattcgtgg catggcggat caaggaatcg
    86461 ttgtattggc agatcaacga ttttctagtt tttcttatcg acgtttattc ccacaacatt
    86521 ggcaaacagc aagagaagta cgaagtgttg gggaattgga agaaggaata cagcgttttt
    86581 ggttatcaaa agaagcagta aatcaaaaaa acgaggaatg acagacgtca gcctcgtttt
    86641 ttgattaacg atagttagtg aattgcaact cgattggtaa gtcaagttta cgtaataaag
    86701 caatcacagc ttgtagatca tcacggttct tacctgtcac tctaatctga tcttcttgga
    86761 tttgtgcttt tactttcagt ttttcatttt tgatagctgt agtgattttc ttcgcgtttt
    86821 cacgatcgat tccgttgacc aaatcggcag tttgtcgagc tttgccacct agagcgtgtt
    86881 ctgattcgga aaaatggata tttttgattg gcacatttct tttgatcagc ttaccaaata
    86941 atacatcttt gatctgttcg attttgaaat catcatcgct caatacaact agattgctgt
    87001 tctctaattt gatttctact gtagaatcct tgaagtcaaa gcgattggac aattcttttg
    87061 ttgtttgctg aatcgcattt tttacttctt ctgtattgac ttctgataca atgtcgaagc
    87121 tagcatcttt tgccatagga accctcctga tatacatata aatatttacc atacatctat
    87181 cttatcatag gattagggca gattcttacc tgtttgctaa aattcttctc atttgagttc
    87241 gaagtatttt attttaatag ataaactgtt attcttacat ttggaatacc aaagtagggg
    87301 agaggtgctt gtgagtaaaa ttatgactgc gtatcataaa aatgaaagat taaagaaaat
    87361 agtggttatt ttgataaccg gattgaccgc tgcggtcgct ttaaattatt ttttgattcc
    87421 agccaatgtc ttttctgcag ggatgaatgg gatcgctcaa atcatttcct cgcttttata
    87481 tgaatggttt catatcaaga tagacacagg aacatttatt tttcttttga atatccctgt
    87541 gtttgtatta gggtttctca aagtaggaaa acgagcaacg atccttagtt ttgtgaatgt
    87601 tgtatgtgta tcggtgatga cgatgctgct tccagtcgga cagatcactg ataatatttt
    87661 gatgaatgcc ctagtaggcg gtgttttatt aggtcttggc gtaggattat ctttaaaaat
    87721 gggctttaca actggtggga tggatatcat ctctcttgta ctttcacaaa caactggaaa
    87781 aacagtcgga aactacatgc tgattctaaa tggtatcatt gtagtcatcg caggcttctt
    87841 ctttaactgg gaaagtgcct tgtatacgat tatttccatt tatgcaatga ctcaagtaat
    87901 tgatgccatc cacacgagtc atcaaaaagt aacagcaatg atcgttaccg tcaagccaga
    87961 agaggtaaca gaagaactag caaaacggat ggtacgtgga atgacattac tgccgtctgt
    88021 cggtggctat tcaaaaaaac aagggaacat gatcatgatg gtcatcaccc gctacgaaat
    88081 gtatgatctt gatcagatcg ttcatgaaat cgacgacgat gcatttatca atattttacc
    88141 aactcattca gtatttggcc ggtttgcaaa tgaaaacgaa caaaaaatgt atcgaacaac
    88201 tggcgtattg ccagaaataa aaaatcgttc acaaaaaaaa tgaggaatca ggtttatttt
    88261 agaataaatt taatctatta catctaataa gaacattttt ataaatttaa acgagaatct
    88321 tacttctaaa ttctcattta caagcagcta aaaaaagagt atgataaata aaaatcgtgt
    88381 taaggagtgt cgtacgatga gcgaagcaaa ccaagaacgg tctggacaag aaatagaact
    88441 aatcaaagaa cgtattcttg ctgccttgga aatggtgatt gatcctgaat taggaatcga
    88501 catcgtcaat ttaggcttga tttatgatat agaattcaat cctgaaaatg gagaaacagt
    88561 cattaagatg actctaacaa ctatgggctg tccattagca gatattttaa cagaatctat
    88621 ccatgacgca ttaaaagaag tgccagaagt aacgaaagca gaagtcaaac tagtctggta
    88681 tccagcatgg acgacagata agatgagccg ttatgcacgg attgcgttgg ggatacgata
    88741 gaaatcttgg ctgactagct atgagaagca aataggaaaa acaaaaaagc cttttatcct
    88801 ttctagaaga aaagagataa aaggtttctt tgtttataaa attttgtctg ctatattata
    88861 agttaattaa ataaatgtgt tctattgata aaggacattc aaatcccttt ccttttggaa
    88921 tccttctttt ttacatcagt ttgattttcg tatagtattc tttctcatct tcacgataaa
    88981 tcgcagcccc atttttttgt tgtgctttaa gactgccaag atttttttta tttacagaca
    89041 aaagtgcctc atcaatgccg attttttttg cctcttgtag tactaatgct aatccttgtg
    89101 tggcatatcc ttttttacga taattagggg agatgccgta accaatatgg ccagcgccgt
    89161 ttcttaatgt ttcgttcagt ttatggcgca aattaaaaat tcctacatac tcatgttcat
    89221 cactttctaa aataaagacg gttgcgggaa cgtaactctc aggaagatcg attcctaaag
    89281 acgttttttc gcattttttt acataatcta aaaattcagc aaatgtatag ccataagcat
    89341 tgttgataaa tccactctca tttggtccaa agctagtttg tagctgataa gctttcttta
    89401 aatctgcttc ccatagtttg atcagtttca tcgtattcac ctctttaagt aaccttattc
    89461 atttgttttc tactatagca ctattgttgt aaatagccat gcctattttt atttattgtg
    89521 tcatatcgta ttaagaatca tcgaacaata caaatatgaa ttacaataaa aaaattattt
    89581 gaagttcgtt cattcgttac ttatgatctc tagaagaaag aaaacgtgaa ttacttcagc
    89641 gtctagaatt ccagaaggat agtaatcaac agaagaaata atcaatatcc aaatgaaaga
    89701 agatagctga atcgagaaaa agaggaaaaa agaaatgaag aaaaaaataa aatagttact
    89761 aatcccagag tcgttcgttt aacattgaaa tcgatcatat ggataaaaca gattttttca
    89821 ccaaccctca atagatgatg gagttgctcg gatgaattat ttgtgtagat ggcataaggg
    89881 agggcgtaca gaaatgaaaa agaacaaaaa tatgttcttg acaaaaaaca catgtaatcc
    89941 taaactaaaa gatgtgatgc ggctatggcg gaattggcag acgcgctagc ttcaggcgct
    90001 agtgagggtg acctcgtgga agttcgagtc ttcttagccg cataatttgt ttttttataa
    90061 agagaaggta cacctggagc gatgagaaag aaaataacat attatctaaa agaatatagg
    90121 aaaaatttta aacaacggaa ccagattcaa ataaatcaaa aaaaatttgt gtgaaggtgg
    90181 gacagtttaa aacctaatag attgattcaa atagtagatt tatagattct gatacaagaa
    90241 atactggcaa acgatagtta aggaataaag actaaactta aa
//
LOCUS       DAFEHS010000003        83735 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFEHS010000003
VERSION     DAFEHS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.003"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            85..972
                     /locus_tag="LP327_000201"
     CDS             85..972
                     /locus_tag="LP327_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL1724351.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="LP327_000202"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="LP327_000202"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBL1724352.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="LP327_000203"
     CDS             2274..2720
                     /locus_tag="LP327_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL1724353.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="LP327_000204"
     CDS             2993..4345
                     /locus_tag="LP327_000204"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBL1724354.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="LP327_000205"
     CDS             4516..4803
                     /locus_tag="LP327_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724355.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="LP327_000206"
     CDS             5029..5472
                     /locus_tag="LP327_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL1724356.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="LP327_000207"
     CDS             complement(5478..6662)
                     /locus_tag="LP327_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBL1724357.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="LP327_000208"
     CDS             6930..7628
                     /locus_tag="LP327_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL1724358.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="LP327_000209"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="LP327_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBL1724359.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="LP327_000210"
     CDS             complement(8438..8971)
                     /locus_tag="LP327_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724360.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="LP327_000211"
     CDS             complement(9268..11649)
                     /locus_tag="LP327_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL1724361.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="LP327_000212"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="LP327_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBL1724362.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="LP327_000213"
     CDS             12350..12889
                     /locus_tag="LP327_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBL1724363.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="LP327_000214"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="LP327_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBL1724364.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="LP327_000215"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LP327_000215"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="LP327_000216"
     CDS             13996..15162
                     /locus_tag="LP327_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBL1724365.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="LP327_000217"
     CDS             15208..16704
                     /locus_tag="LP327_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBL1724366.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="LP327_000218"
     CDS             16785..17468
                     /locus_tag="LP327_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL1724367.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="LP327_000219"
     CDS             18113..19303
                     /locus_tag="LP327_000219"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBL1724368.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="LP327_000220"
     CDS             19405..20358
                     /locus_tag="LP327_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBL1724369.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="LP327_000221"
     CDS             20427..21776
                     /locus_tag="LP327_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1724370.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="LP327_000222"
     CDS             complement(21959..22282)
                     /locus_tag="LP327_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724371.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="LP327_000223"
     CDS             22411..23574
                     /locus_tag="LP327_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL1724372.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="LP327_000224"
     CDS             23644..24993
                     /locus_tag="LP327_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL1724373.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="LP327_000225"
     CDS             complement(25038..25469)
                     /locus_tag="LP327_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1724374.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="LP327_000226"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="LP327_000226"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBL1724375.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVCEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="LP327_000227"
     CDS             28699..29295
                     /locus_tag="LP327_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL1724376.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="LP327_000228"
     CDS             29417..30565
                     /locus_tag="LP327_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL1724377.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="LP327_000229"
     CDS             complement(30603..31016)
                     /locus_tag="LP327_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBL1724378.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="LP327_000230"
     CDS             31095..31526
                     /locus_tag="LP327_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBL1724379.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="LP327_000231"
     CDS             complement(31625..31858)
                     /locus_tag="LP327_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBL1724380.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32041..33711
                     /locus_tag="LP327_000232"
     CDS             32041..33711
                     /locus_tag="LP327_000232"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBL1724381.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="LP327_000233"
     CDS             33858..34244
                     /locus_tag="LP327_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724382.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..34929
                     /locus_tag="LP327_000234"
     CDS             34231..34929
                     /locus_tag="LP327_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL1724383.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34934..35398
                     /locus_tag="LP327_000235"
     CDS             34934..35398
                     /locus_tag="LP327_000235"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBL1724384.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35395..36306
                     /locus_tag="LP327_000236"
     CDS             35395..36306
                     /locus_tag="LP327_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL1724385.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36541..37080
                     /gene="pyrR"
                     /locus_tag="LP327_000237"
     CDS             36541..37080
                     /gene="pyrR"
                     /locus_tag="LP327_000237"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBL1724386.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37094..38377
                     /locus_tag="LP327_000238"
     CDS             37094..38377
                     /locus_tag="LP327_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBL1724387.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38390..39316
                     /locus_tag="LP327_000239"
     CDS             38390..39316
                     /locus_tag="LP327_000239"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBL1724388.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39335..40615
                     /locus_tag="LP327_000240"
     CDS             39335..40615
                     /locus_tag="LP327_000240"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBL1724389.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40620..41696
                     /gene="carA"
                     /locus_tag="LP327_000241"
     CDS             40620..41696
                     /gene="carA"
                     /locus_tag="LP327_000241"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBL1724390.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41701..44886
                     /gene="carB"
                     /locus_tag="LP327_000242"
     CDS             41701..44886
                     /gene="carB"
                     /locus_tag="LP327_000242"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBL1724391.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44940..45716
                     /locus_tag="LP327_000243"
     CDS             44940..45716
                     /locus_tag="LP327_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBL1724392.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45713..46645
                     /locus_tag="LP327_000244"
     CDS             45713..46645
                     /locus_tag="LP327_000244"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBL1724393.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46642..47358
                     /gene="pyrF"
                     /locus_tag="LP327_000245"
     CDS             46642..47358
                     /gene="pyrF"
                     /locus_tag="LP327_000245"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBL1724394.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47365..47994
                     /locus_tag="LP327_000246"
     CDS             47365..47994
                     /locus_tag="LP327_000246"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBL1724395.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47982..48296
                     /locus_tag="LP327_000247"
     CDS             47982..48296
                     /locus_tag="LP327_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724396.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48334..48975
                     /locus_tag="LP327_000248"
     CDS             48334..48975
                     /locus_tag="LP327_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL1724397.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49018..49710
                     /locus_tag="LP327_000249"
     CDS             49018..49710
                     /locus_tag="LP327_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBL1724398.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49707..50609
                     /locus_tag="LP327_000250"
     CDS             49707..50609
                     /locus_tag="LP327_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL1724399.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQADIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50767..52473)
                     /gene="efbA"
                     /locus_tag="LP327_000251"
     CDS             complement(50767..52473)
                     /gene="efbA"
                     /locus_tag="LP327_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBL1724400.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52663..53286
                     /locus_tag="LP327_000252"
     CDS             52663..53286
                     /locus_tag="LP327_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724401.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53736..54785
                     /locus_tag="LP327_000253"
     CDS             53736..54785
                     /locus_tag="LP327_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL1724402.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54782..55663
                     /locus_tag="LP327_000254"
     CDS             54782..55663
                     /locus_tag="LP327_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1724403.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55666..56436
                     /locus_tag="LP327_000255"
     CDS             55666..56436
                     /locus_tag="LP327_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1724404.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56715..57569
                     /gene="aroE"
                     /locus_tag="LP327_000256"
     CDS             56715..57569
                     /gene="aroE"
                     /locus_tag="LP327_000256"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBL1724405.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57747..58910
                     /locus_tag="LP327_000257"
     CDS             57747..58910
                     /locus_tag="LP327_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL1724406.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59027..59437
                     /locus_tag="LP327_000258"
     CDS             59027..59437
                     /locus_tag="LP327_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL1724407.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59611..61839
                     /gene="topB"
                     /locus_tag="LP327_000259"
     CDS             59611..61839
                     /gene="topB"
                     /locus_tag="LP327_000259"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL1724408.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            62010..62294
                     /locus_tag="LP327_000260"
     CDS             62010..62294
                     /locus_tag="LP327_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724409.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            62454..62690
                     /locus_tag="LP327_000261"
     CDS             62454..62690
                     /locus_tag="LP327_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL1724410.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62687..63085
                     /locus_tag="LP327_000262"
     CDS             62687..63085
                     /locus_tag="LP327_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBL1724411.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63752..64696)
                     /locus_tag="LP327_000263"
     CDS             complement(63752..64696)
                     /locus_tag="LP327_000263"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL1724412.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64992..65936)
                     /locus_tag="LP327_000264"
     CDS             complement(64992..65936)
                     /locus_tag="LP327_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1724413.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66157..67371
                     /locus_tag="LP327_000265"
     CDS             66157..67371
                     /locus_tag="LP327_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL1724414.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67393..69408
                     /locus_tag="LP327_000266"
     CDS             67393..69408
                     /locus_tag="LP327_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBL1724415.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69491..70864)
                     /locus_tag="LP327_000267"
     CDS             complement(69491..70864)
                     /locus_tag="LP327_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL1724416.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70890..71174)
                     /locus_tag="LP327_000268"
     CDS             complement(70890..71174)
                     /locus_tag="LP327_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1724417.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71176..73191)
                     /locus_tag="LP327_000269"
     CDS             complement(71176..73191)
                     /locus_tag="LP327_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL1724418.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73370..73675)
                     /locus_tag="LP327_000270"
     CDS             complement(73370..73675)
                     /locus_tag="LP327_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBL1724419.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73764..74795)
                     /locus_tag="LP327_000271"
     CDS             complement(73764..74795)
                     /locus_tag="LP327_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL1724420.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75002..75877
                     /locus_tag="LP327_000272"
     CDS             75002..75877
                     /locus_tag="LP327_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL1724421.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76029..76871)
                     /locus_tag="LP327_000273"
     CDS             complement(76029..76871)
                     /locus_tag="LP327_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL1724422.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76963..78840)
                     /locus_tag="LP327_000274"
     CDS             complement(76963..78840)
                     /locus_tag="LP327_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL1724423.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78837..80258)
                     /locus_tag="LP327_000275"
     CDS             complement(78837..80258)
                     /locus_tag="LP327_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL1724424.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80261..83650)
                     /locus_tag="LP327_000276"
     CDS             complement(80261..83650)
                     /locus_tag="LP327_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL1724425.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
BASE COUNT        26775 a        14820 c        17289 g        24851 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaaat aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta tgcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34501 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34561 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34621 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34681 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34741 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34801 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34861 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34921 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34981 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    35041 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    35101 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    35161 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35221 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35281 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35341 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35401 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35461 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35521 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35581 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35641 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35701 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35761 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35821 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35881 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35941 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    36001 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    36061 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    36121 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36181 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36241 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36301 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36361 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36421 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36481 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36541 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36601 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36661 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36721 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36781 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36841 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36901 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36961 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    37021 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    37081 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    37141 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37201 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37261 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37321 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37381 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37441 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37501 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37561 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37621 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37681 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37741 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37801 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37861 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37921 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37981 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    38041 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    38101 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    38161 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38221 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38281 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38341 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38401 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38461 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38521 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38581 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38641 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38701 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38761 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38821 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38881 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38941 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    39001 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    39061 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    39121 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39181 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39241 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39301 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39361 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39421 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39481 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39541 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39601 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39661 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39721 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39781 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39841 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39901 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39961 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    40021 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    40081 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    40141 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40201 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40261 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40321 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca
    40381 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40441 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40501 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40561 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40621 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40681 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40741 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40801 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40861 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40921 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40981 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    41041 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    41101 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    41161 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41221 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41281 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41341 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41401 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41461 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41521 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41581 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41641 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41701 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41761 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41821 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41881 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41941 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    42001 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    42061 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    42121 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42181 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42241 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42301 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42361 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42421 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42481 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42541 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42601 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42661 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42721 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42781 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42841 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42901 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42961 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    43021 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    43081 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    43141 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43201 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43261 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43321 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43381 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43441 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43501 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43561 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43621 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43681 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43741 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43801 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43861 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43921 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43981 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    44041 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    44101 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    44161 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44221 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44281 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44341 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44401 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44461 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44521 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44581 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44641 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44701 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44761 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44821 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44881 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44941 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    45001 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    45061 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    45121 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45181 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45241 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45301 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45361 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45421 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45481 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45541 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45601 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45661 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45721 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45781 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45841 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45901 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45961 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    46021 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    46081 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    46141 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46201 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46261 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46321 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46381 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46441 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46501 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46561 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46621 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46681 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46741 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46801 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46861 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46921 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46981 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    47041 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    47101 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    47161 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47221 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47281 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47341 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47401 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47461 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47521 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47581 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47641 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47701 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47761 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47821 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47881 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47941 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    48001 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    48061 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    48121 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48181 taatctcttc tcttttttct attatatata gattttctct attttttggt aagggagctg
    48241 tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag ttatttaatg atatgatatt
    48301 cggaaaaaga aatctcaaag ggaggtaact tttatggggt tgaatgaatt tattttagga
    48361 ttgaacaatt tagagacgat cacaagggca cctggatttt ttaaatatac agagcataca
    48421 gttgcagcac attcgtatcg tgtagcatct attgcgcaag ttctaggaga cattgaagaa
    48481 gcgaatggac aaccgatcga ttggaaactg ctctatgaaa aatcattgaa tcatgactac
    48541 acagagcgtt ttattggtga tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga
    48601 gggatgctgc aaacagttga agaaaagatg acggacgaat tcatcaacca agagattcca
    48661 gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa
    48721 ggagaactat tagccatttc agataaggtt gaccttcttt atgaatcttt tgatgaaatc
    48781 atcaaaaata acccggaaaa agtttataag gagatgttca ttgaggcagt gactacgatt
    48841 aaaagctttg aacatcggcc ttctgttcag tatttcttta aagagatatt tccggaacta
    48901 ttgaaccagg aattttatgg aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag
    48961 aaagaagaaa aataaaaatg atatgagatg cagggggaag tatagttgaa aaagaatatg
    49021 gatgtcgaat ggggctattc tggagacaaa ggtccggaaa attgggcgag tttatgtgat
    49081 tggtttgcga gaggtgcgga atttccattg cagtcgccta ttcatttgac caagggggaa
    49141 gaaaccaaaa tagaaggaga cacgctttcc tttcattatc aaaagcaacg gtttactgac
    49201 aaggaattca aaaacacgat tcatttagta ccttttgacc aactcagtta tgtagagttc
    49261 aagaaagaac gctattattt gacagatatt catttccact tgccttctga acatatcatc
    49321 aacggaaaac aggaagatat tgaatttcat tttgttcata tgaacagcaa aaaagagaat
    49381 ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag gttggctgta taatcaagaa
    49441 aatggggaaa gttggaactt agaaaaacat gagcattggg taaatccgga tgtgttcttt
    49501 ccggagcaaa aaagccattt ccattatatt ggctcattga caactccgcc taccagcggg
    49561 ccaatccaat ggtttgtaat ggatcatgta ggaaaactga accaagagtt tcttctcccc
    49621 ttcgatggtc agtatgctcg accgaacaac cgaccaatcc agcccttgaa tggaagagaa
    49681 atttattatt tcgaggagtt tgagcaatga atattcagca gatgaaatac gtggtcgctg
    49741 tagcaaacaa tggtagtttc agagaagcgg caaagaaaat gtttgtctcc cagccaagtc
    49801 tttcagctgg tatcaaagaa ttagaaaccg agttagggat cacgctgttt actcgaacga
    49861 atcgaggggc atatttaaca gaagaagggc aagacttctt aaatcgtgcc gagaaaatat
    49921 tggtccagct tgatacatta gaaactcact atctaacgag tgaaaagaga gacaattttt
    49981 cgatcgctgc ccagcattat gatttcttag gtcctttgac cgcacagcta ctcgaggaat
    50041 tcaaagacga gatcaaacaa tttcggatca tggagacgac aacagcgaga gtgatcgaag
    50101 aagtcaaaga gcagcacagt gagatcggca ttatttatat gaatgagcat aatcaagctg
    50161 atatcagaag atatttacaa caaggagagc taagtggccg cagcattgga aaattcaaaa
    50221 cgcatatttt tttaaggcaa ggacatccat tagctgaaca cgcagagatc accaaagaag
    50281 aattgttatc ttatcctcaa gttcgtttta cacaggatgg aaataatttc ccgtattttt
    50341 atgaagacct tatcgagatc cctgatcaag aaacagtgat ttatacgagt gatcgaggta
    50401 ctttgatgaa tatcgttctt gaaactgatg cttacgcttc aggatctggt attgtgattg
    50461 gagggattcg cgagcacttg cggttaatcc ctttggcaga aggcgaacta aatgaatttt
    50521 atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc
    50581 ttactcggtt actggaccag cagagctaag aaaagcagat gagaacgctg tttattgaac
    50641 accgttgatc aaaaaagagg ggttgtgaca gaattttgtc acaactcctc tttttgtagc
    50701 aaacaattta taactaattg ctgttgtggg aaagtacgca aatatgattc tagaaacact
    50761 cattgattac ctgttttgtt tcatagctgt aattttttct tcttctggtg tcacgataat
    50821 cgtcttttgg ttttcataaa taacataacc aggcttggcg ccatttggtt ttcggatatg
    50881 ctttacctgt acaagatcaa ccggcacttg ggcagagtat cggtattttg aaaaataggc
    50941 agctaactcg gcagcttctg taattgtttc atctgagggc ttgtcacttt taatgatgac
    51001 gtgcgaaccg ggtatatttt ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat
    51061 tgtcagctgg tcattttgaa gattgttttt accaacaaga atgagcgttc cgtcagaaga
    51121 aaagtattga tcaggctgag atggtttctt acgtttttgt ttttttaagt tcttcttttt
    51181 caggtatcct tctgaagtca attcttcttt aattgcttcg atatccattg ggccggcaat
    51241 ttccagttga gataagacag attcaagata ttggatttca tctttggctt cttggatttg
    51301 ttcgccaatc agcttaacag cgttcttcag cttttggtat cgatggaaat atttctgtgc
    51361 attttgattt ggtgtcaatg ccggatctag tgctattttg attggccgat cttcttcata
    51421 ataattaggt aaaacaactt cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag
    51481 tagttcgcca tttctacggt aattctcagc gttttcggat tctttgagtg tttgttcacg
    51541 ttttttcaat ttatttttat ttcgttttag ttcattttcg atttttcgaa tcagttctcc
    51601 gccttgttgt tttgcccgat ctttctctgc tttttcgtga taatagctat ctaataattg
    51661 actgagtgtt ggataagctg ttacagcaga tgcctgttcg gacaatacct gataagcaat
    51721 cggtgtgaaa aaatctttat tttccgtttc gtaaaaagta ggaataggct gatggctgat
    51781 ggctgagaaa aattcgtccc atacaaccat cttttcatta ggacgttcca gtaatctttt
    51841 ggtcagctct tgagcgatat cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg
    51901 atctagttca gtttgggaaa gccgctgaaa gattttttct ttctcggagc taaatgggtt
    51961 caagacctct tgttttggcg gagcaacata ttctacacca ggaagcaaag aacgataagt
    52021 gttttgagag cttccaatgt gtttgatcgc atccagtatt tttccagttt ctcgattaac
    52081 taagacgatc gtactatgac gtcccattaa ttcaacgatc aatatgatat tttgtagatc
    52141 acccaactcg tttcgtttag caaagtggaa atggatcact cgatcatttt cgatttgttc
    52201 gatgtcttct aaaatagcgc catccaaata ttttcgaagc atcatcacga agttaggcgg
    52261 attatcagga ttctgataat cgatttgggt aatctgtaca cgagcataac ttgggtgcgc
    52321 agataataat aatcgatggt ttttgccgcg tgaacgaatc accagcacga tttcattttc
    52381 ataaggctga tggattttcg agatccggcc agatagtaac gtttcacgca attcgttgac
    52441 catcgcgtgg gtaaatacac catcaaagga catggttcat tcctcatttc ttttcataag
    52501 tatattcatt ataacgaaaa aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag
    52561 tctagaaaaa tcttggacta attacataaa aaacagtgtt tctatcgttg aaatccaaat
    52621 ttttaggtat aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa
    52681 gtagttaaat cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat
    52741 ccaagtaagt attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt
    52801 gcaatgtttt tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg
    52861 tccataccaa ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa
    52921 caaagccggg cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg
    52981 cttgatgttt ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat
    53041 ccgatcgttt atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg
    53101 acatttattt tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga
    53161 ataactgatt ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc
    53221 aaacaattag aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct
    53281 aattgagaca ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga
    53341 acaagtgatg tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa
    53401 ttcaaaaatt cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt
    53461 atcaaaacgt atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt
    53521 attaatggtg agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat
    53581 ctatgctaga catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta
    53641 aagacaatga ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata
    53701 ataaataaaa agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag
    53761 ttgtagtaat tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg
    53821 aaaaagaaag tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat
    53881 acgtgagcca tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag
    53941 aaggattaaa agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga
    54001 gcaagctagc cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa
    54061 tcgcaacacc tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag
    54121 gtgctgtaac ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta
    54181 atgtaacagg tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt
    54241 tgttgccaaa tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat
    54301 accaagtagc acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg
    54361 cggtgccttc cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt
    54421 ttatctatgt tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag
    54481 caaacaaagc aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac
    54541 tagcaacaat cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag
    54601 aacttgcgag tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg
    54661 atacagtgat caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga
    54721 aggaaaaagc caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca
    54781 aatgatcgta tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt
    54841 catgacttat cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg
    54901 tgctgctgtc tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt
    54961 aggcgtaggt gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa
    55021 gataccggtt attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt
    55081 tgtgatgcga acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca
    55141 tcgctttggg ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat
    55201 cttgattgct gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac
    55261 aggagacaac gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt
    55321 aggtttaact ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga
    55381 cggatatgca gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat
    55441 catcggtgaa gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt
    55501 cggcagtatc atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac
    55561 ttatctgaag ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa
    55621 gaaagcactg aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg
    55681 acaatcaaaa atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg
    55741 gatgaaatta accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt
    55801 gctggaaaaa gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg
    55861 atctctttta aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt
    55921 tctcgagttt tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat
    55981 ttgctacttg cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat
    56041 caaaaagaag attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg
    56101 gatactccag cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg
    56161 acgatcacag ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa
    56221 acagctaaag cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt
    56281 ctcatgatca cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc
    56341 caaaaaggga aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc
    56401 gaccttttgc agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag
    56461 tagaaggact tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata
    56521 aacaattaaa catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa
    56581 atagaatatt ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc
    56641 tagacgtttc gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac
    56701 aaaggaggaa taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg
    56761 cttctcacag tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg
    56821 cggtgtattt agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc
    56881 gaacttttga tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt
    56941 atctggatga ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg
    57001 aaaagggtcg tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag
    57061 aagctgggat ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa
    57121 aggcagtcac tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa
    57181 acaaccaaac attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt
    57241 gtaagataaa agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa
    57301 gctgtcttct agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc
    57361 tcttagatat aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta
    57421 gagaaacacg tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg
    57481 gcatgttgat ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga
    57541 tccatgtgat tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct
    57601 cgcataaacg gtctcaagaa aagtaatcct ttatgaatcg aagcatttat ttcgttattt
    57661 tttattaaat tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta
    57721 tcagtaagaa acgaggagga accacaatga gtcggatgga tcgctataag gacatccata
    57781 agaaggcgaa acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac
    57841 gaacgcgtat ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat
    57901 ttgaacattt gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac
    57961 aacaaaaaaa taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa
    58021 gaaagcgatc gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag
    58081 gtatctcatt ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga
    58141 cttcagaaac ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg
    58201 gaagtgatac acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg
    58261 atggtccttc ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc
    58321 caggggtagg acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc
    58381 gtcaaacttt agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca
    58441 aatcgtttga aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa
    58501 aagatctgaa tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg
    58561 ttcttttgca gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac
    58621 gccaacagca agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc
    58681 gtgcacctta tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt
    58741 tcttgacgaa aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg
    58801 tgccagtaga cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa
    58861 ttgataatca aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa
    58921 gatcaagaag aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa
    58981 aaacgctttc ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct
    59041 gaacttcaaa aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga
    59101 agcatcaggt atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga
    59161 agaagcaaaa aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt
    59221 tgcagatacg ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt
    59281 catccagaca agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg
    59341 taagattgag atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga
    59401 tcgatttggc atcaactggc agattaacca agagtgacag ctaaagatga atattttctg
    59461 gaaacatcaa gcaccaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg
    59521 actatacggt atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc
    59581 cgcaaaaaat taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct
    59641 tcacaagcaa aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat
    59701 gaaatcagcg atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg
    59761 gtagatatgg ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat
    59821 ttgcctattt tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt
    59881 tcgatcgtaa aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat
    59941 cgagaaggtg aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa
    60001 aagaactatt tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt
    60061 aagaatctcc aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag
    60121 aacgctgatt ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga
    60181 aaagggattg atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt
    60241 tatcagctgc aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt
    60301 ttaattacga caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa
    60361 acccaagaag agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac
    60421 ggacggattc ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg
    60481 ctatctagtt tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt
    60541 gatgctgctc aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt
    60601 tacattacag aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt
    60661 ttaggaatag aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg
    60721 aaaaaagttc aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc
    60781 tttgcgaagc tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg
    60841 gctatgtttg ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa
    60901 ttaaagctaa aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc
    60961 atcaagtcaa aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca
    61021 gaagtagaac tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga
    61081 acgttgatca cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa
    61141 attctcaaag aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat
    61201 cttaagcaga agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga
    61261 atcactcttt gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg
    61321 cgctgggaag ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta
    61381 gagaatatca aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag
    61441 gtagactttt cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac
    61501 gatcaattag gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc
    61561 gctacatgct tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag
    61621 ctgactaaaa cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa
    61681 ggttttactg gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca
    61741 atcggttttt cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa
    61801 gaaacatcaa gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg
    61861 aatatttatt ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat
    61921 caagcaaaaa cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga
    61981 aaaaagtgca ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc
    62041 aagcatttct aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa
    62101 cagcatattc accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt
    62161 atttttttca taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg
    62221 tggtggtagc ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc
    62281 aatattttgg ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt
    62341 tgtcattttt tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac
    62401 ttggatataa tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa
    62461 aaatgaaaaa gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag
    62521 aagtaaaaga attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg
    62581 atggttctgt acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt
    62641 cgatcatgaa tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc
    62701 gactaaagaa attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat
    62761 gatcggtgta aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt
    62821 attcaatcaa gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt
    62881 agctaaaaaa catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt
    62941 gtttcttcaa ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat
    63001 cataatgatt accactagtt cattggattt cgattcacta aagtccaaga taaccagtta
    63061 tttacgacaa accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc
    63121 atgtcattaa ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata
    63181 aaaatatgga aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt
    63241 gaatagtagg aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac
    63301 ataatatata ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt
    63361 atcaaaaatc gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc
    63421 agtttttctt actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt
    63481 taatcaaata tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt
    63541 taattaacca cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt
    63601 catatgttca tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt
    63661 tgcttaccta ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt
    63721 tttaggattt ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta
    63781 aagcacttgt tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa
    63841 cttcacgaac accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt
    63901 cgtattcacc agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag
    63961 cttttacgat acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa
    64021 tgatctcata agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa
    64081 tacgatggtc tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc
    64141 atgcagcaac ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt
    64201 caacttttaa tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag
    64261 taccgataac acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat
    64321 ctactgggtt tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta
    64381 cgatttggcg catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt
    64441 tttggttgat accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac
    64501 ctgaccatac agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt
    64561 ccccttctgc tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt
    64621 tgatcattgc ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac
    64681 gacttgtttt tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa
    64741 agtatagtct tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt
    64801 tttgatggaa acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat
    64861 atcccctgat aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc
    64921 aacgatataa taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa
    64981 agattttctt cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg
    65041 aataatttga ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac
    65101 gaaaaactcg tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt
    65161 tcttgaaagg aagcatcaaa atacattttc aatttattac taagatagac cggattgtag
    65221 ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct
    65281 aatactctaa gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct
    65341 aataaatgag tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta
    65401 tgactgtatg tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt
    65461 gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt
    65521 atcaaaaaag aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat
    65581 aaggtttctt cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt
    65641 ctttgagtag cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct
    65701 ccattaataa tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt
    65761 gtatgctcgg gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga
    65821 aatagatccc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca
    65881 ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa
    65941 aatgtctaat tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct
    66001 tattagttca cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt
    66061 ctatggttgg gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact
    66121 atatttttga taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg
    66181 aaattttctc tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca
    66241 attcccagta tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg
    66301 actgctccct cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa
    66361 atcggacaaa aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc
    66421 cccctcttct cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc
    66481 tttggattga tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa
    66541 cgagcctcta tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca
    66601 tttgttgcag gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc
    66661 agcttaccta ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt
    66721 caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt
    66781 tatggtattt ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc
    66841 acagtcctga cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc
    66901 atgcaattgg caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat
    66961 cgctatatgc ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact
    67021 gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta
    67081 ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc
    67141 aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg
    67201 ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt
    67261 atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag
    67321 acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg
    67381 aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac
    67441 tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg
    67501 ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt
    67561 cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat
    67621 ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt
    67681 atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa
    67741 tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg
    67801 ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat
    67861 ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat
    67921 ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga
    67981 gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca
    68041 agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact
    68101 ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt
    68161 tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta
    68221 tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat
    68281 gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga
    68341 acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac
    68401 tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa
    68461 tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg
    68521 aatgaattag ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa
    68581 atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc
    68641 atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact
    68701 catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat
    68761 gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat
    68821 gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta
    68881 aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt caaattctat
    68941 gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat
    69001 tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact
    69061 caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat
    69121 gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca
    69181 aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat
    69241 tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg
    69301 attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta
    69361 gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg
    69421 cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt
    69481 tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa
    69541 agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac
    69601 cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg
    69661 tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa
    69721 tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc
    69781 cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg
    69841 tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa
    69901 agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt
    69961 tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca
    70021 ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca
    70081 gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag
    70141 ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat
    70201 ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc
    70261 caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg
    70321 caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag
    70381 ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc
    70441 ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa
    70501 gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta
    70561 gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat
    70621 tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta
    70681 gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga
    70741 tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa
    70801 tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat
    70861 ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt
    70921 tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata
    70981 gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat
    71041 acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca
    71101 agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact
    71161 gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt
    71221 tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc
    71281 caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg
    71341 atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac
    71401 ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat
    71461 aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt
    71521 attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg
    71581 atccgataat tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc
    71641 cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc
    71701 tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg
    71761 cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc
    71821 caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag
    71881 aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc
    71941 tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat
    72001 caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc
    72061 ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc
    72121 ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc
    72181 gtagatggaa gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga
    72241 ataggaaata cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg
    72301 aaacgaagca aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca
    72361 ttccagcaaa aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca
    72421 aagagagaaa aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac
    72481 ttgtcgacta atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc
    72541 catggatgtt tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat
    72601 tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac
    72661 tttggcgtaa taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc
    72721 aagtgactgc atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga
    72781 ccctagcagc ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa
    72841 ttcatttctt atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc
    72901 ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc
    72961 tcttcgctct tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc
    73021 ccagtcgatt gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa
    73081 aggagttaat tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc
    73141 tagactgatt tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt
    73201 cacctcctac ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac
    73261 ttacagttgg atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa
    73321 aaagcaggtg accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat
    73381 catgtttatt ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt
    73441 cgatatcttt ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg
    73501 aaccagataa agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt
    73561 cttctttgtc ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt
    73621 taggggccat tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg
    73681 ttcctcctag tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa
    73741 caaacccgat tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt
    73801 ccttcttcca aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg
    73861 ccattaacta atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa
    73921 attacttttt cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt
    73981 tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct
    74041 tcttgtcgat cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt
    74101 ggacgttctt tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa
    74161 aaatccagat cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag
    74221 ctgatatcat ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt
    74281 ccatataaat agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg
    74341 aaataatcat aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact
    74401 ttttggactg tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga
    74461 cctttttctt ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt
    74521 ttctctacta gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat
    74581 tgactaggtg tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc
    74641 tgatcagtat attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt
    74701 tttttgatat tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta
    74761 ctctttccat tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt
    74821 tttcagtata tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt
    74881 tcagaaaaac acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa
    74941 atcaaaaaga tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat
    75001 catgcttatt gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat
    75061 tgtcgattac atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc
    75121 aaaagctacg tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag
    75181 cggatttaaa gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt
    75241 caatcaaacg attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc
    75301 aaagaaaatg gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca
    75361 tgaagattta gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc
    75421 aaaaggtgat tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa
    75481 gtttttgatc atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag
    75541 cgaggattgc gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat
    75601 gacctatttg aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga
    75661 aatggccaaa caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa
    75721 agtagcgact ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt
    75781 gatatattct caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat
    75841 gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata
    75901 agaaagatag tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc
    75961 tttttcaaac ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg
    76021 aggaatagct aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg
    76081 aggaaaatca gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat
    76141 actttctgta cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca
    76201 agtaagcgat gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc
    76261 cctttttcga ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact
    76321 tgataagcga gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc
    76381 ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt
    76441 gtattttctc cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc
    76501 agcacagtat tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa
    76561 ataccgatcg tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta
    76621 gtaaagggat ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc
    76681 atgttttctt ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa
    76741 tgagagatga tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct
    76801 agtatcccta ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc
    76861 tttggtttca tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat
    76921 ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt
    76981 gcgtctagca aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac
    77041 atagattccc ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc
    77101 aggagaaatc aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg
    77161 gtttgttaat ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata
    77221 ttttagacct gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc
    77281 tttcgccgat acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt
    77341 gtcactatct tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac
    77401 gtcaccatct actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt
    77461 ttgatcattt gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc
    77521 tgctttttcg ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga
    77581 aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata
    77641 atttactggg tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga
    77701 atcattatca aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt
    77761 gttttgcgtt ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg
    77821 aattttttca ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt
    77881 agacgtatca tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa
    77941 aggcgtgatt gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa
    78001 attatatttt ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc
    78061 ggcaaagtca ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt
    78121 ttgatcagtt gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct
    78181 tggtgttcct tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc
    78241 agtaccgatt tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag
    78301 atggatagta tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat
    78361 ctcatataca gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc
    78421 ttctttgatc gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac
    78481 atttccatca gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac
    78541 tgcttgtttt gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt
    78601 gacgttataa actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat
    78661 ttctttcccg ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag
    78721 gatgacttga gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag
    78781 taatggtaaa ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat
    78841 tttttcactc ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata
    78901 ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta
    78961 ttttcttttt tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa
    79021 gttgaaatgg taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc
    79081 tcttttaatc gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg
    79141 gaaatcaggt gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc
    79201 tggaggtaat ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct
    79261 ggtttatttg tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt
    79321 gtactgacac caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt
    79381 gctgtactgc ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc
    79441 aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct
    79501 tctgtcagct tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct
    79561 tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc
    79621 gtatgcaagg aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat
    79681 accttctcaa aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata
    79741 gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc
    79801 ttttctttga cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt
    79861 tcttgatctt tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta
    79921 gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt
    79981 gcttcttcta ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa
    80041 acttggaatg tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg
    80101 tcatttggat gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc
    80161 tgaaccattt ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac
    80221 aagaagagac acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt
    80281 ttttcaaaaa aagataccaa atagaaaacg cacaaccaat catccctact agatagattc
    80341 ctaaacggcc aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta
    80401 aagaaatctg gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca
    80461 cttgaattga cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc
    80521 cttctggtgt aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa
    80581 aggtatctgt ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc
    80641 tgaattttgc tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa
    80701 acgcttttag tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt
    80761 ttcccgtgta aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag
    80821 tatcatatcc ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat
    80881 caaaactggc aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat
    80941 ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc
    81001 cttgcatcgc atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca
    81061 actggatcgt atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag
    81121 aagcgatctt gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg
    81181 tttctgtcag cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg
    81241 ttccgtcgcc atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag
    81301 cgccaataag atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg
    81361 tgaattgttt agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta
    81421 caggtaattc tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta
    81481 gtgataatat ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac
    81541 gttcccacgt atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc
    81601 aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat
    81661 cagctagatt gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag
    81721 ctttagcaga tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag
    81781 ttgtcctgcc gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga
    81841 ttctcacttg gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct
    81901 tgatggtatt tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac
    81961 tcttgactga aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt
    82021 gtccattgct tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat
    82081 ctgttgcatg atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt
    82141 gtcgcatacg agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt
    82201 ggatgccaag accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg
    82261 ttgcgataaa cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga
    82321 ttcttgagat tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt
    82381 aataattgct gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac
    82441 catccgtcag caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg
    82501 catctcttag tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt
    82561 ttacttgatt tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat
    82621 atccttcgct tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg
    82681 ctaaagtatc gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt
    82741 tcatacttcc tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta
    82801 catttccacg agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat
    82861 actttcgtat cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg
    82921 tccgatcatt aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt
    82981 tattgccttg atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat
    83041 aagtgccctg atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca
    83101 cagctggcaa ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg
    83161 atgattcttc ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt
    83221 ctgtctcatc aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt
    83281 ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga
    83341 tttccaacgt aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt
    83401 ccacatcact taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg
    83461 tgttattcgc atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt
    83521 cagctgaaat tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg
    83581 gcacaaagtt tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg
    83641 tggtcgtcat ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt
    83701 attaaaataa acaggcgtta cataatttta tttat
//
LOCUS       DAFEHS010000004        77295 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFEHS010000004
VERSION     DAFEHS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.004"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            598..1287
                     /locus_tag="LP327_000277"
     CDS             598..1287
                     /locus_tag="LP327_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1724426.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="LP327_000278"
     CDS             1274..2482
                     /locus_tag="LP327_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL1724427.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="LP327_000279"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="LP327_000279"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBL1724428.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="LP327_000280"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="LP327_000280"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBL1724429.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="LP327_000281"
     CDS             complement(6585..7280)
                     /locus_tag="LP327_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBL1724430.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="LP327_000282"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="LP327_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBL1724431.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="LP327_000283"
     CDS             complement(8479..8670)
                     /locus_tag="LP327_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBL1724432.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="LP327_000284"
     CDS             complement(8691..9581)
                     /locus_tag="LP327_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBL1724433.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="LP327_000285"
     CDS             complement(9568..10335)
                     /locus_tag="LP327_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL1724434.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="LP327_000286"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="LP327_000286"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBL1724435.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="LP327_000287"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="LP327_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBL1724436.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="LP327_000288"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="LP327_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBL1724437.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14906..15289
                     /locus_tag="LP327_000289"
     CDS             14906..15289
                     /locus_tag="LP327_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724438.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="LP327_000290"
     CDS             complement(15457..16215)
                     /locus_tag="LP327_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBL1724439.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="LP327_000291"
     CDS             complement(16233..17051)
                     /locus_tag="LP327_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL1724440.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="LP327_000292"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="LP327_000292"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBL1724441.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="LP327_000293"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="LP327_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBL1724442.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="LP327_000294"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="LP327_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBL1724443.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="LP327_000295"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="LP327_000295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBL1724444.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21070..21312
                     /gene="yaaA"
                     /locus_tag="LP327_000296"
     CDS             21070..21312
                     /gene="yaaA"
                     /locus_tag="LP327_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBL1724445.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21299..22423
                     /gene="recF"
                     /locus_tag="LP327_000297"
     CDS             21299..22423
                     /gene="recF"
                     /locus_tag="LP327_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBL1724446.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22420..24366
                     /gene="gyrB"
                     /locus_tag="LP327_000298"
     CDS             22420..24366
                     /gene="gyrB"
                     /locus_tag="LP327_000298"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBL1724447.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24386..26857
                     /gene="gyrA"
                     /locus_tag="LP327_000299"
     CDS             24386..26857
                     /gene="gyrA"
                     /locus_tag="LP327_000299"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBL1724448.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27093..27392
                     /locus_tag="LP327_000300"
     CDS             27093..27392
                     /locus_tag="LP327_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBL1724449.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27442..27954
                     /gene="ssb"
                     /locus_tag="LP327_000301"
     CDS             27442..27954
                     /gene="ssb"
                     /locus_tag="LP327_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL1724450.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27980..28216
                     /locus_tag="LP327_000302"
     CDS             27980..28216
                     /locus_tag="LP327_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBL1724451.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28359..30332
                     /locus_tag="LP327_000303"
     CDS             28359..30332
                     /locus_tag="LP327_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBL1724452.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30338..30790
                     /gene="rplI"
                     /locus_tag="LP327_000304"
     CDS             30338..30790
                     /gene="rplI"
                     /locus_tag="LP327_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBL1724453.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31064..32431
                     /gene="dnaB"
                     /locus_tag="LP327_000305"
     CDS             31064..32431
                     /gene="dnaB"
                     /locus_tag="LP327_000305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBL1724454.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33211..34503
                     /locus_tag="LP327_000306"
     CDS             33211..34503
                     /locus_tag="LP327_000306"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBL1724455.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35239..36102
                     /locus_tag="LP327_000307"
     CDS             35239..36102
                     /locus_tag="LP327_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL1724456.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36231..37853)
                     /locus_tag="LP327_000308"
     CDS             complement(36231..37853)
                     /locus_tag="LP327_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1724457.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38102..38623
                     /locus_tag="LP327_000309"
     CDS             38102..38623
                     /locus_tag="LP327_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL1724458.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38809..40539)
                     /gene="spxB"
                     /locus_tag="LP327_000310"
     CDS             complement(38809..40539)
                     /gene="spxB"
                     /locus_tag="LP327_000310"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBL1724459.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40704..41522
                     /locus_tag="LP327_000311"
     CDS             40704..41522
                     /locus_tag="LP327_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBL1724460.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41625..41822)
                     /locus_tag="LP327_000312"
     CDS             complement(41625..41822)
                     /locus_tag="LP327_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724461.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41991..42569
                     /locus_tag="LP327_000313"
     CDS             41991..42569
                     /locus_tag="LP327_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL1724462.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42664..42801
                     /locus_tag="LP327_000314"
     CDS             42664..42801
                     /locus_tag="LP327_000314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724463.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43614..44117)
                     /locus_tag="LP327_000315"
     CDS             complement(43614..44117)
                     /locus_tag="LP327_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL1724464.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44476..45501
                     /locus_tag="LP327_000316"
     CDS             44476..45501
                     /locus_tag="LP327_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL1724465.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45848..46726
                     /locus_tag="LP327_000317"
     CDS             45848..46726
                     /locus_tag="LP327_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBL1724466.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47202..47318
                     /locus_tag="LP327_000318"
     CDS             47202..47318
                     /locus_tag="LP327_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL1724467.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48246..48584
                     /locus_tag="LP327_000319"
     CDS             48246..48584
                     /locus_tag="LP327_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724468.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48678..48908
                     /locus_tag="LP327_000320"
     CDS             48678..48908
                     /locus_tag="LP327_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724469.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49308..49679
                     /locus_tag="LP327_000321"
     CDS             49308..49679
                     /locus_tag="LP327_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBL1724470.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49953..50645)
                     /locus_tag="LP327_000322"
     CDS             complement(49953..50645)
                     /locus_tag="LP327_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL1724471.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50648..51433)
                     /locus_tag="LP327_000323"
     CDS             complement(50648..51433)
                     /locus_tag="LP327_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL1724472.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51670..53070
                     /gene="lacG"
                     /locus_tag="LP327_000324"
     CDS             51670..53070
                     /gene="lacG"
                     /locus_tag="LP327_000324"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL1724473.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53095..53412
                     /locus_tag="LP327_000325"
     CDS             53095..53412
                     /locus_tag="LP327_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL1724474.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53425..55098
                     /locus_tag="LP327_000326"
     CDS             53425..55098
                     /locus_tag="LP327_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBL1724475.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55112..56743
                     /locus_tag="LP327_000327"
     CDS             55112..56743
                     /locus_tag="LP327_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBL1724476.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57615..58805
                     /locus_tag="LP327_000328"
     CDS             57615..58805
                     /locus_tag="LP327_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBL1724477.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            58939..61128
                     /locus_tag="LP327_000329"
     CDS             58939..61128
                     /locus_tag="LP327_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL1724478.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            61125..62918
                     /locus_tag="LP327_000330"
     CDS             61125..62918
                     /locus_tag="LP327_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL1724479.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            63118..63432
                     /locus_tag="LP327_000331"
     CDS             63118..63432
                     /locus_tag="LP327_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL1724480.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            63453..63830
                     /locus_tag="LP327_000332"
     CDS             63453..63830
                     /locus_tag="LP327_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL1724481.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            63840..64613
                     /locus_tag="LP327_000333"
     CDS             63840..64613
                     /locus_tag="LP327_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724482.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            64635..66038
                     /locus_tag="LP327_000334"
     CDS             64635..66038
                     /locus_tag="LP327_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL1724483.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            66220..67404
                     /locus_tag="LP327_000335"
     CDS             66220..67404
                     /locus_tag="LP327_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL1724484.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            67401..67691
                     /locus_tag="LP327_000336"
     CDS             67401..67691
                     /locus_tag="LP327_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724485.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            67688..67909
                     /locus_tag="LP327_000337"
     CDS             67688..67909
                     /locus_tag="LP327_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724486.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            67951..68730
                     /locus_tag="LP327_000338"
     CDS             67951..68730
                     /locus_tag="LP327_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBL1724487.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            68797..69291
                     /locus_tag="LP327_000339"
     CDS             68797..69291
                     /locus_tag="LP327_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL1724488.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            69347..69985
                     /locus_tag="LP327_000340"
     CDS             69347..69985
                     /locus_tag="LP327_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724489.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            70057..70452
                     /locus_tag="LP327_000341"
     CDS             70057..70452
                     /locus_tag="LP327_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL1724490.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            70436..72889
                     /locus_tag="LP327_000342"
     CDS             70436..72889
                     /locus_tag="LP327_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL1724491.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            72886..74913
                     /locus_tag="LP327_000343"
     CDS             72886..74913
                     /locus_tag="LP327_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724492.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            74910..75932
                     /locus_tag="LP327_000344"
     CDS             74910..75932
                     /locus_tag="LP327_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL1724493.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            75949..76878
                     /locus_tag="LP327_000345"
     CDS             75949..76878
                     /locus_tag="LP327_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL1724494.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            77123..77239
                     /locus_tag="LP327_000346"
     CDS             77123..77239
                     /locus_tag="LP327_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL1724495.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT        24510 a        13812 c        15263 g        23710 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtat attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt
    20941 atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata
    21001 aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag
    21061 ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg
    21121 ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac
    21181 actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg
    21241 atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat
    21301 gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt
    21361 ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga
    21421 aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat
    21481 tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct
    21541 tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca
    21601 gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct
    21661 ttctttagta aaaggcagcc cgcagaccag gcggaagttt ttagatatgg aattaggcca
    21721 gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa
    21781 ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt
    21841 gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa
    21901 gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct
    21961 ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca
    22021 agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac
    22081 gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac
    22141 ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga
    22201 tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga
    22261 gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt
    22321 cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt
    22381 ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta
    22441 gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc
    22501 gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt
    22561 gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc
    22621 catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct
    22681 gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat
    22741 gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga
    22801 tcttctgttg ttaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat
    22861 gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa
    22921 acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca
    22981 actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc
    23041 gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat
    23101 tatgacggcg ggattaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt
    23161 ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa
    23221 tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa
    23281 ggcggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc
    23341 cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa
    23401 ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa
    23461 acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc
    23521 atgaaattct tgctggaaaa cccaagtgtg ggccgtcaaa ttgttgaaaa aggattacta
    23581 gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct
    23641 ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa
    23701 tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat
    23761 cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc
    23821 atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg
    23881 tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat
    23941 gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg
    24001 cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa
    24061 caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa
    24121 gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga
    24181 gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc
    24241 cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat
    24301 cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat
    24361 atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt
    24421 caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc
    24481 acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg
    24541 catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg
    24601 agatgtcatg ggtaaatacc atcctcatgg agactatgcg atctatgagt caatggtacg
    24661 gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc
    24721 tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc
    24781 tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga
    24841 tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac
    24901 aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt
    24961 agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt
    25021 attacctgga ccagattttc caacaggtgg tttggttatg ggaaaatcag gaatccgaag
    25081 agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat
    25141 gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa
    25201 attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt
    25261 gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag
    25321 cgcatctgtt atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt
    25381 caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaattcttga
    25441 aaactatatc gaacatcagc gcgaagtcat cacaagacgg acagtctttg aaaaagaaaa
    25501 agcagaagca agggcacaca ttttagaagg tttgcgtatt gctttggatc atatcgatga
    25561 aatcattgcg attatccgcg ggtctaaatc agatgacgaa gcaaaagcga cgatgatcga
    25621 acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt
    25681 gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga
    25741 agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt
    25801 agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt
    25861 cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa
    25921 caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg
    25981 tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc
    26041 tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata
    26101 cgaaattcca gaatacgggc gcacagccaa aggaatacca attatcaatt tactaggtat
    26161 cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca
    26221 ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc
    26281 taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa
    26341 tgtgctactg acagatggtc aatctaatat cattattgga acacataacg gctactctgt
    26401 cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg ttagaggaat
    26461 acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaag atcaagaagt
    26521 attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa
    26581 aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc
    26641 tggattgacc actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat
    26701 tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt
    26761 gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc
    26821 agaacaaaca gttgaagaac aaggatcaga agaatagcca tcaagactaa ttgtgtaagc
    26881 tagcaagaat gaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa
    26941 tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta
    27001 taatcagagc ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta
    27061 agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt
    27121 acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg
    27181 caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt
    27241 tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc
    27301 catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca
    27361 ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa
    27421 cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa
    27481 gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg
    27541 aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt
    27601 tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt
    27661 actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca
    27721 gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa
    27781 caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac
    27841 tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac
    27901 ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat
    27961 ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt
    28021 agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa
    28081 aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa
    28141 aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt
    28201 cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc
    28261 aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt
    28321 atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt
    28381 tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa
    28441 ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc
    28501 gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg
    28561 cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga
    28621 ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca
    28681 aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa
    28741 aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa
    28801 agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga
    28861 acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat
    28921 tggatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga
    28981 tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac
    29041 agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag
    29101 cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa
    29161 gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg
    29221 gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg
    29281 ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt
    29341 tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata
    29401 tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag
    29461 cttacatcgt tatcaacgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa
    29521 tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga
    29581 aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc
    29641 agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag
    29701 agtttccaag taaacctctg ctgacttata tcgaatcttc agcttcttca gcttcagagc
    29761 tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg
    29821 ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg
    29881 cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac
    29941 aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa
    30001 gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata
    30061 gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt
    30121 tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga
    30181 tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta
    30241 ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag
    30301 aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga
    30361 tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa
    30421 ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc
    30481 aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt
    30541 aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga
    30601 cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata
    30661 caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta
    30721 cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt
    30781 acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa
    30841 gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg
    30901 atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt
    30961 ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca
    31021 ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca
    31081 ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct
    31141 tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg
    31201 tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga
    31261 cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt
    31321 aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc
    31381 gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt
    31441 tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag
    31501 tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact
    31561 gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct
    31621 accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat
    31681 cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa
    31741 tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc
    31801 tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg
    31861 tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc
    31921 aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg
    31981 gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat
    32041 tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa
    32101 aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt
    32161 agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga
    32221 acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg
    32281 agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa
    32341 gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt
    32401 ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc
    32461 gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta
    32521 aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac
    32581 ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat
    32641 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt
    32701 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag
    32761 cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc
    32821 ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga
    32881 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    32941 tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt
    33001 tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac
    33061 ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat
    33121 aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata caatggttca
    33181 ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa
    33241 tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt
    33301 gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat
    33361 aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac
    33421 ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt
    33481 gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc
    33541 aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa
    33601 ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta
    33661 ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa
    33721 tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga agaatattat
    33781 gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct
    33841 ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac
    33901 cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga
    33961 agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca
    34021 cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc
    34081 gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat
    34141 tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg
    34201 atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc
    34261 gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat
    34321 gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca
    34381 gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca
    34441 ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc
    34501 taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat
    34561 ctatacgata ataataactt gaacactgtt tattcagaat aagggactgt gacaaaaacc
    34621 gtgtcacagt ccctttttcc gaacaaacgg tgttcaaaag cttgcatcta cggcactaag
    34681 cccaatcaga tgaaaaatat ggagtgctat ttttatctga ttgttcttac tgcttgatgc
    34741 agagcagctt tttcacaacc tctccactaa acggtgttct cctgccaact cctcggtcac
    34801 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgttcct tgtgactcgg
    34861 agctaaacgg caggctcaca acctcttggt aaacggtgtt caatcagctg accctcggta
    34921 ttagctcaaa aaacggcaaa atatgaggaa ctattttttc cgtttttttc tctaatactc
    34981 aggtcaaaac gctgatttca caacctctga ttcacggtgt tccagaagta gcttctacgg
    35041 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    35101 ttgaagctga ccacttctgt cacaacctct tattttgatt agaacttgcg aaaataaacc
    35161 ggttaaagtg tgtctagcgc aagagatttg ctataataga cgataagggt aaaatgtaac
    35221 gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga tctgccatac
    35281 catgtattag caaaagaaaa agttgatttt ttatcaatga atctccaatt gaatgatcaa
    35341 gagttagtag atgacctcgg aaagacattc aattatgacc gctacatgaa tcagctgaaa
    35401 aatggtgcga tgcctacgac atcacaaatc aatgttggcc gatatttaga atttttccgt
    35461 tcttatgcgg aaaaacagat tcctgtttta tatgtggctt tttccagtgg attaagcggc
    35521 tcttacagta gtgcgcttca agcggttgaa ttgctgaagg aagaatatga agaccctgag
    35581 atctatgttt ttgatacaaa agctgcaagt attggggaag ggcttttagt attggaagct
    35641 gtaagattga aaaaagaagg taaaagcatc gaagaaaccc tagagtggct ggaaagtaat
    35701 tatttgaaag tccattcatg ggtaacagta gacgatctga aatatctaga acgaggcggt
    35761 aggatttcca aagcaagtgc tactcttggc agtctattga gtgtaaagcc tattattacg
    35821 gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa caagtcgatc
    35881 cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca aatcgtttat
    35941 gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt ggaagagcag
    36001 ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag tcacacaggc
    36061 tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta actcattctg
    36121 taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat agatgagcat
    36181 cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca ttatgcccat
    36241 aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata tgtcgtatca
    36301 gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa tgtttggata
    36361 aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa gccgtcattc
    36421 aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac aagtacgtta
    36481 gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc agataaaaca
    36541 ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa gcttcttaag
    36601 aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa aatcgttaga
    36661 tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt tgtcacaccc
    36721 caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa tagcgttgtt
    36781 gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa attaaaagat
    36841 atatcatcta aaattttctt tccatcaatc gttacactta cattttctac ctgcaataag
    36901 tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct agaagaaggt
    36961 tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc ttgttttgat
    37021 tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat ttgttcttct
    37081 tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc caaccaaaaa
    37141 tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat atgtgtacat
    37201 actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc aaaattgatc
    37261 aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg ttcgtctagc
    37321 aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac cttttgtcca
    37381 ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag accttgcagt
    37441 aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc tccttctaat
    37501 tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat cgcatctttt
    37561 tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag tacagtatag
    37621 tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg tcccatagta
    37681 acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt tgatttacct
    37741 gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat attcacatca
    37801 tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat caattttttt
    37861 cctcactttt atcattatta gttttgacaa gtcttataga attatatagg ggaaaggtaa
    37921 aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga aaaaaataaa
    37981 aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca gcataatttt
    38041 tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag aaggattgaa
    38101 aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt tatttgtact
    38161 tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg tagtagtctt
    38221 tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca gattaaaaga
    38281 gctaacagga tataaaggaa agatgccctt aataatcggg atcatcgata ttctagttgg
    38341 cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg tttttgcagt
    38401 atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa gaggagtaag
    38461 tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat taggaatttt
    38521 cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg gattctattt
    38581 tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat caaacaaata
    38641 aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct tttttatcat
    38701 ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa aaatgtgaga
    38761 agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc acaacctctt
    38821 ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt tgaatgcttg
    38881 gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg gcaatgggcg
    38941 cttgttattg atcttcacat cgattactac aggacgatct gattttgcag ctgcatcaaa
    39001 ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga gcgcttctcc
    39061 aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt ccgtatcttc
    39121 ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa taattggaag
    39181 ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc catcaccact
    39241 taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc ctggcacacc
    39301 attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt tcatattcaa
    39361 taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg catcgtcttc
    39421 agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt cctctcgatc
    39481 ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg ctaggtacca
    39541 gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg tgtttgcatc
    39601 accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat cgatatcgat
    39661 ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt cgctaccgac
    39721 aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag cgacacgtcc
    39781 agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta gaacggaagc
    39841 gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc cgtttcgcgt
    39901 tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca aagcagctag
    39961 caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg tggagaaagg
    40021 ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag ccacgccttt
    40081 atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt gtggtgtcat
    40141 gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta gttcctggaa
    40201 agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta gaggcacatg
    40261 gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc ctgcagagcc
    40321 gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga gtgcgccgac
    40381 ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata gcgcatccat
    40441 aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc agctttcaag
    40501 aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact cctttagcaa
    40561 tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa atcccaacta
    40621 attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt agtacataga
    40681 tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa ccttggcagc
    40741 aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc gggttggttt
    40801 ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt gcttctggtg
    40861 tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa gcagaagaaa
    40921 atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt gtgtttttat
    40981 atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa gcagaaggat
    41041 tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg cataacattc
    41101 ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat aatccgacag
    41161 ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac ttcccagaag
    41221 gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag gcctttgttt
    41281 acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg ctactagtaa
    41341 cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca atgatttatg
    41401 tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa cgtcattttg
    41461 cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg gcattaggat
    41521 aaataaaaaa aatatatatg atatatcaaa tagggccgcg aacttatatc gcgaccctat
    41581 ttttttagta agttatatca taatgaagtg aatggtatca tacattaata atagtttttt
    41641 ctatgtgtat ttcctttgat ccagcgaaca agccagatga taagtgcgat tgggattaga
    41701 actttgaccg caaaccataa aagcccgctt aggacactga caaaaagact gatgacgaag
    41761 ctagctgcaa ttagcactaa aagaaccatg aagatattta agaacatatt tttttgacgc
    41821 atacaaaacc ctcctcttgt tcattcttta ctatagaaaa agtataacag ggattgttta
    41881 aaaacacctc caactgaaga ctgattttta cttcatctac ttaaatgatc aaacaattgc
    41941 tttttctctg gtttatcatc taaaatagag cggaaaagga ggagaatatc atgcctaaaa
    42001 atcgtcttga agcatttaca gatgctgtaa tcgcgattat catgactatt ttagttttag
    42061 aacttcatga acctaacgga gacacgtggg aggcgctcgc tgcattaggt taccgatttt
    42121 ttatctatat tgtcagtttt atcattttag cgatttattg gaacaatcat catcatttat
    42181 ttcaagttgt acataagata aatggacggg tcttatgggc gaacagtttc tttgtttttg
    42241 cgctttcgct ttttccgttt gcaacttcat ggatcagtga gtacatcaat agcctggctc
    42301 cagaaatgac ttatggtttt gttaccttga ccgctaatat tgcgtattat atcttggcaa
    42361 aagaacttgt caaagcaaat aaaggccacg agaaagcatt gaaggcgatg ttgaataatt
    42421 acaagaaatc gtatctatcg attggtttga atgtgttagg aattgtctta gggtatttag
    42481 tagcgccagt agccgtgctg atcgcaaaca tcgtgattct agctgcttgg atcatcccaa
    42541 gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt aagagaaaaa
    42601 tatagaaaaa ataaataaac gatcaaatat ccgagtgata ctagtattca aagagggatg
    42661 cagatggatc aaaaaagaat tcatgtagga ctgattacta gttttgtcac gatgcttatt
    42721 tatttggatc aaggtcattt gacttggaca gtagttggtg tcgtaggatt tttgacatta
    42781 ttcacagcaa taaaaaaata aacatcttta tgtaatctga tgttctatct atttaagaaa
    42841 agcatgttta cagcagtatg attctgttgt aaacgtgttt ttatttgtgg aaaatttaat
    42901 ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc cacagtggat aacttcgtaa
    42961 gagataaaaa taaaaaatta gtcaaagaag ggcgtataga tttcttttga taggagatat
    43021 acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt gtggtttttg
    43081 gaataaaaaa ataacatatc tcattttacg cttttgttat tttatactta aaaaataacg
    43141 acacagaaaa taccgtgtct cagatttctc tactacttct aaaaactact gtttttttcc
    43201 tttgaaatgc cttcaaaaag taggatcagg cagaatatac tggcagaatc aaggagaagg
    43261 gagcctaaat aaggagaaaa accatagaaa atataaagta attacttata aaacaagctt
    43321 tttatagaaa aataatatac ctgagtaggg tataaattaa gacggaattc ctttctagat
    43381 tgagatattg actaggaaga atgttaagat tttaagcctt taagctaaaa aaaaaccctt
    43441 tttgttaaat aaggggaata tagcctgtca gtttcaaact gcaagtcgtc ctgctgcaat
    43501 aatatagtag attagagatg aaaatagatt tttttaaacc aatgactgaa taaaaagacc
    43561 gtcatagata cacattggaa tgtgtctatt acggtctttt tttacgaacg attttattga
    43621 agacggacag ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa taaaatgtat
    43681 cccaaaataa tggctagacg gtctaactta tttgtcgata gatgtttgtt tagaagttga
    43741 taggcccata aacctatcag tcctcctgtt gtattagtta tcaaatcagt gatgtccgct
    43801 acgccaatac cgaataggaa ttgaatcaat tctgccaaga agcttacaag gaaaataatc
    43861 cctaattttt tagaaaaaga aagtctcttg aaattgacat tcaaaagcac cccaaaagga
    43921 aaaaagacaa tcaagttata agtggtctca cgtaataccg tcgtattttc aaaagaaaaa
    43981 gtagaaaaag ggacgagatt gacattgctg taagaataag tgagaaattg tggaagatta
    44041 aatgatagct tgaataaaat caaccataat agaaagaata aataaatccc taataaaaat
    44101 tttgatacat accgcataga catctcctcc atttaaaaag tataccatcg aaaaccgctg
    44161 aaaaatcata aaaaagtatg aagtttaagg accaacgtag ttatcgtacg aaaattagaa
    44221 gctttttttt gttgggaatc cggtttcttt aacaaagcta agatagcgag ttgcttggaa
    44281 aagttgctat cttttttctg tcaaaacgac tcggaatgtt cgttttttgt tctaatttct
    44341 ccaagttgtt tactttttga tactaagttt atgaaaaatc aagaaatagt tgttatacta
    44401 ggtaatgctg acaaacggga ttggcatttc atcttggttc agcttttttt ccaattacta
    44461 ggaaggaggg atagattgtt caagacttat tttcgtttca ataaaataca agataatcct
    44521 tttcgtatca tgaatctgat gctacttatc tttattgtat tatggttagg ttacataaat
    44581 catgattttt tgatttcaag tttttcatca ttagggatct ttactttttt ctattatcaa
    44641 aacataccga tgaaatattt gctgaaacgt atgtttctga tcggttgcgg actgttggtt
    44701 gctttcgttt tggggatctt atcgacttat gttttatggc ttgaaccatt tgccgtagct
    44761 ttggttgctt tttccagcag attcgtgttg cgattattcc atatttctaa accaggtggc
    44821 ttgttttttg cgatgctttc tgccatggga acgagcatgc agctgccaat cgctcaatta
    44881 ccgatcgtct cactttattt ttttatgggt gtcgtttttg ccctgattgc ggcagtgatt
    44941 acaaaattac tggattcacg gcctgagcag acaattgaaa aagcaacttt aaaagaaaga
    45001 tttcacgaag agcctttagt gatcattgat tctgttttct acagcgctgc cttatttctt
    45061 tccgtttatg tcagtcacgg cttgaatctg cataatcctt attggttgac cttgtcatgt
    45121 gcatcgattt tgctagcaga aaacttggat gcgatgaagc atcgacaagt ccaatatctg
    45181 attggatcga tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc gtttacgcag
    45241 ttgcagacca tttttctgat cacttttcta tatggaatcg cacaatttct agttgctaga
    45301 aattatgcag ttgctaatat ttttcttaat ccgatggcgt tgatgctatc tacacttatt
    45361 cgtggagcat acttgatttc tttgatcgaa tatcgttttt tagggatcgt cataggaagt
    45421 ttcatcggtt taggtgttgc gtgggtaatg acggttggtt tgcaacatta tttaacggtt
    45481 gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc aaaagaggtt atgacaagat
    45541 ttttgtcaca acctctaatt caaggtgttc aaaagccaga ttctcagcat taagtcaaat
    45601 tcacacaaaa atgtggaaag gaactttcaa aaaaatttta tatttattct atcaaaaaat
    45661 tttatattta ttctatcaaa aaattttgtt tatgatggta actgaataaa ctttcctgag
    45721 atggtcaatg ctttccttga aagtcttaca gatattcttt cttcatagaa agtttaagtt
    45781 ttatagttag tgtacaataa aagtatgttt ataccatatg gtgtccggat aatcagtcat
    45841 ttaaacaatg aggttcaaag atatgaaaag gggaaatttg atgaagaaaa taagcaatga
    45901 aatccaaatt ctggcagaaa ctttaactct tcccaaagtc gttatagagc aaatgattgt
    45961 acaagcaaaa aacatccttg atgaggtgtt tgagcaatat agtcatgaac taatggatcc
    46021 taataaagca aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc
    46081 agcattagct atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt
    46141 gaagattcca agaactatct atttggcaac aatgaatgct tttactagat ttattcatga
    46201 acatttccaa gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac
    46261 tagtggcttg atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg
    46321 gtacacggat ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc
    46381 agatttatct cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact
    46441 ttattttcct gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa
    46501 tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt
    46561 attatcagtt gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc
    46621 ccaaatacat gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct
    46681 tgcaggcgag cacattggct ctggcttagg tattcttact tcataagttt agtacttcga
    46741 aatagtttaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    46801 gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
    46861 gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc
    46921 tcttttttag tgttctatcg aacacacagt acgttcaata ggtaaactag ctataacaat
    46981 agatattaaa gttaacggac aagagggcct tttttaggta gctaaaacgt aataatgaaa
    47041 atttatttcc agagatagga gtcaatcagc tagtctgatc gactgtttta acaagttgac
    47101 tttcatttta acttttgata ccatttaaaa cacaaatcgt aattgtttcg atttgtgttt
    47161 tttagcatga ctacaatcaa agaagaaagt gggtcgtcca tatggaaaaa acagatatct
    47221 caagtgcata ccgccgcttg aaaagcccaa atatcaaaac acgaaaacgt gcgctgaaga
    47281 tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga agaaggaaac ttaattaaaa
    47341 aaatagccga aaaacgtttt tcggctattt tcaataatcg gtttttgtgt aaggtttccg
    47401 aaagaactgc tgttgatttt ccatttttaa catatgataa attggtagaa tctttctttt
    47461 caaattgata ccattcccta attccagtta tatagttaac tacagaacca caaagcacgt
    47521 acgtttataa aagtattatc tgtagttatt tttcaattcc ctttcaacct cttattttga
    47581 ccagtagtat tgatcacttt accttttaac acctttgttc gtttttgaaa catttttagt
    47641 caagaatgga aacgtaacta cataatcaaa ggttttgcga aatatttcat gaaatatttc
    47701 atgtagaagc gagcaatacg gttgcaaata accattatcg tttaataagc agttacgaaa
    47761 gctcccaata tctctaacgt tcagctcact ccatttcttt tcttcaaaaa aatcagtgat
    47821 tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa agtaacgccg aaaaaatatg
    47881 caaaaaaata aaaaagcagt tactattgct agaataacat caaggaataa taagttttgg
    47941 ttttcagata atagtcgata agttgtgtat tggaatttta gatacagtaa taaagtattt
    48001 ttcatgaaat tttgtgagaa tcactattac aagtatcttt aataaaatta atggttaagg
    48061 aggaacttaa agtagaatgt aaatgaatag ttgtagtcag tcgattgtga tactagactc
    48121 aaagttgcag gaataagttt ttcaatttgt gattggaaga taaaaaatga aaaagaaggg
    48181 ctttttttta tactttgttg attgtagtgt ttattttccc tctgtttagc caagtaacat
    48241 tttctatgac tcaaccagtt tcaagtgagg aacatgataa cctttttcgt gctacagaag
    48301 agtttaagaa atatcaggaa aatattgttc cggagactcc attagctagt gagccaatta
    48361 ctgatattga tggaaaccaa ataggatggt atatacagta cgaagttatg gtggtaaata
    48421 atgactcaat agtaaaaaat acagtaggct tagagctaac accgattgaa aaggtgagtt
    48481 cattgttaac gtatatttat actgatgaaa gtgacgagac tgagatcatt ttaacggatt
    48541 actcaaaaat tgctactgat aaaaagtata tattgtggat gtagacacta gtgaaatact
    48601 ttatgatata gaaattgatg aaaatttact agatgtagca caagaaatcg atcatcaaaa
    48661 gcaggaagct attcgatatg ctggtagaaa agggaaaaga aaatatttat tacgtgaatg
    48721 tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt caagagtcgc tactcaatta
    48781 actcgactcc gacatttaca gtttacagag aaggatcgat agaaaaaact gttttttgga
    48841 ctaaagaaag tggaatgtct ttagctgaag tagaagagtt tttggattat gttagtatgc
    48901 aacagtaagg gagttcttat tatgaaaaat tttgacacat attatgttat tgttttttcg
    48961 atatctttgc tgttaatatt actaacttta atgcagtata aagagtatcc agagtatttt
    49021 attttaactt ttgtatattt agtaagtagc tacacacttc tatataaatt cagaagttct
    49081 tctagagttt ctaaagtttg tttcttaatt agcttattga ttctataata tttttgattt
    49141 atataggaaa ttaaaaatat tatagaatca acactcagag aacgggttgg tttttaatag
    49201 atttaataag tactgataat gaaatttctc ctggttggga aaacgaagtt ggttaccctg
    49261 tagaagaatt ttttcaagat tactttgcgg agtatgccga taggataatg agagattacg
    49321 taacagataa aactcaagaa gtcatcaatc aatcccagtc attaaatgaa ttatctgata
    49381 aggcgaaaga agaagcagtt gccattattt cttcagggat tagtgactat attgatatga
    49441 gtggtgaaat caattatggc gataaagaca aaattgtacg catgataaaa gcttttatca
    49501 aaacttttga tgtgaaggca gatcttaagt ttaatgatac ttacctagta ggaaaattaa
    49561 ttaatatcct tgggacagag aaagatacgg aaacgtttat ttcctctttg tctatgttgt
    49621 ttacagataa gcatttttca actcagcttg gtggagagga actagtggaa atcttttaac
    49681 cattacgccc agcattaata aaagtatatc aaaaaagtac tcctgatgat ttttaagatt
    49741 ggtttgagaa ataatgtgaa aacactattt ttaatgttaa ttatagatag agatgaacgc
    49801 gtcatatggt taaaagaatg aaaaagtcaa agatacactg ctatatttca ctaaggatat
    49861 agcagtgtat ctttcatgtc gtttcactgg cagtttttta taaaaggagg tgtgcatttc
    49921 agttgctttt ctttggtagt tagttgaatc gtttaagaat taacggatgt ggcagacagg
    49981 tgctctgtcc aaaaaccagt aaaatctttg atggttccat ctttatttag ctgtgcctgt
    50041 tcaaatactg ttttgttttt accgaatgga tcaattgcaa taattgttcc gatccccgca
    50101 cgtttggcag caagtaatcc tgagtaagca tcttcgataa caagacaatc agccggagct
    50161 aagctcagtt tgtctgccgc tttaagaaaa atatccggtt gcggtttccc agggaaagag
    50221 ccgtcgtcat agacaatctt gtcccagtca aaccagcggg ctaaatcgaa aatatcaaaa
    50281 taaaaagcaa cattctcttt gacagtagcc gtggcaatcg ttaatggaat ctgttggtca
    50341 gtcagctggt caagagtcgc ttcaagccct tttgtcaact gaagctcgtt ttcattttgc
    50401 aagcaaagca tacgataata gctttctttt tcttctgaca atacggcgat ctcagaggcg
    50461 gtcaaatgct ctgaaatgaa atgtttcaaa atctccgtat tcgtccgacc atgaatattt
    50521 tttaaaatat cctcgtctga taaaccatct tttgtatgat catgtatcat atgcagccaa
    50581 gcagcttcat gcagatgaga gtccaaaaac attgttccgt tgaaatcaaa tataatccct
    50641 ttcatagtta cgctcctata gtggtatttt cggttgaatc agcaatttgc agatcgacat
    50701 attgtgcata gtgctcttga acctcccgcg agatctggcg atccgtaatg agcagatcga
    50761 aatcatgcaa attataaaac acataaaaat catctcgatt gaatttttta ttatcaacga
    50821 gtagatattt cttacgggaa ttatttagag cgatctgctg tgcttctcct tcttcggtac
    50881 tgtaagtaga gatcgcttcg ttgtgaacgc catttgaact gataaatgct tttgtaaact
    50941 ttagtcttct taaattgtcg ttggtgatcg gtccgacaaa tgcgccggta tttttccgga
    51001 aatcaccgcc aatcagtaga atttctggtc catctgcttg agcaagaata tcaaaaactg
    51061 gataattatt tgtaatgatc cgaattttct tgttcagtaa atgcgtcgcc aatagttcga
    51121 ttgttgtacc tggtcctaag aaaatcgttt ctccctcgtg aatcaaggag gctgctgtgg
    51181 cagcaatttc ttttttttct tgaatctgca gagtggcttt ttcagtgtga gaaagttctt
    51241 gatctaaagt gtaggaaaga ctttgagccc ctccatggac acgcagcagc ttgcccgatt
    51301 tttctaattc atctaaatct ctgcgaacgg tcatatcaga aacatctaat tgattgatga
    51361 tttcattaac ggtaaggatt ccttttttat tgaccatttc tacgatagta agcaatcttt
    51421 cttttttcaa cattgtcttc acctctaatt taaggataat ctatcatata aacggacaac
    51481 agtcaatcta attcgaacaa aagtaaacat ttagggtggt ttttatgttt gttacaatag
    51541 attttattgc tgttttctgg tttttatgat tatttgtgtt tgtttttgtt gacaaatgtt
    51601 ttggatgcgt ttacaatgca atcagttaga aagagcgaaa actgattata gatttgagga
    51661 gagatgtaca tgcttaaatt accagaagat tttatttttg gcggagccac agcagcctat
    51721 caggttgaag gagccactaa ggaaggtggc aaaggcgctg ttgcttggga tgacttttta
    51781 gaggaacaag ggcgattcag tcccgatccg gcaagtgatt tttatcatca atatgcaaag
    51841 gacattgaat tgtgtgaacg ttttggtgta aatggtctaa gattatccat tgcttggagc
    51901 cgcatttttc ctgatggagc tgggaaacca aatccagaag gaatcgcttt ttatcatcgt
    51961 gtgttcgaag aatgtaagaa aagaaacgta acgccgtttg tgacgttgca tcattttgat
    52021 acccctaaac gtttgtttga ccatggagat tttttgaatc gagaaacaat agaagcattt
    52081 gtttcctatg ccattttttg ttttcatgaa ttcaaagaag tcaaggtgtg gagtaccttt
    52141 aatgaaatct atccagttgc aaccaatcaa tatttgttag gtgttttccc accaggaatc
    52201 aaatatgatt ttacaaagat cgtcgcctgt cttcataata tgatggttgc tcatgcccgt
    52261 gtagtgaact atttcaaaga aaacgaactt ccgggtgaaa ttggtgtagt tcactcatta
    52321 gagacgaaat atgcggcaac agacgcgccg gaagataagc atgctgcctt tttagacgat
    52381 gcattgtcga tccgtttctt attagatgct acctatttag gctattattc aactgaaacc
    52441 ttgactgctc ttgatgagat atgtgaagca aatcaggcca gctatcattt tcctgaagaa
    52501 gatttcgttg aattgaaaaa agcatccacg cgtaatgact acttagggat caatcactac
    52561 caatgccatt ttgtcaaagc ttacgatgga gaaaatgcaa tccatcataa cggtacgggt
    52621 gaaaaaggga cctctgttta taaagtgaag ggaatcggag aacgtattta taaagaagga
    52681 attcctcgaa ctgattggga ctggctgatt tatccagaag gtctatatga cttgttgctg
    52741 cgaattaaaa gcgattaccc acattacaac aaaatctata tcactgagaa cgggatgggg
    52801 tataaagatc agtttgaaga tggcattatt atggatcagc cgagaatcga ctatttgaga
    52861 gtctatttgg aatccttaag caaggcgata acagccgggg taaatgtgaa ggggtatttt
    52921 ttgtggtcgc ttatggatct tttctcatgg acaaatgggt ataacaaaag gtatggcttg
    52981 ttttatgttg attttgaaac ccagaagcgt tacccaaaag aatcagctta ctggtacaag
    53041 ttggtcagtg aaaccaaaac gattatttaa taagtgataa tggaggaaga aaagatgaat
    53101 aaagaggaat tacaaatgct tggttttgag atcgttgcat attcaggcga cgctcgcagt
    53161 acattattga aattattgaa agaagtaaga caaggaaact ttgaacatgt tgattcggcg
    53221 ttgaaagaag ccgacgaaaa tttaaccctt gcacataatt cgcaaacaaa aattttagcg
    53281 gaagaagcat ctgggaaaga gatggagatg ggattcattt ttatccatgg gcaggatcat
    53341 ttaatgacca ctttattgct gagagatctg attcaagact ttattgtact gtacagacag
    53401 aatcaaggat aggagaagaa cgctatggat ggaatcatta aacaaatcga aaaagggaaa
    53461 ccattttttg aaaaaatctc acgtaatagt tatttaggag ccgttcgtga cggattttta
    53521 acagcgatgc cagctatttt attttcaagc attttcattt tgattgctgc ggttcctgag
    53581 atttttggtt accaatggcc tgaatctgtc tccacatggc tatggaaagt ctataattat
    53641 tcgatgggtg ttgtagggtt actcgtttca gcaaccactg ctcgctgctt ggctgattcg
    53701 atgaatcgtc gcttaccgaa aaacaaagta atcaacacca cctctgtgat gctggcctct
    53761 gtggtaagtt tcctgttgtt aagcgtctct ccgattgaag gtggattttc aacggaatat
    53821 atggggacaa agggactact ggcttccttt gtcgctgcat ttttaacagt taatttatat
    53881 aagttttgtg tgctgaaaga tattaccatt aaaatgccta aagaagttcc tggaacgatt
    53941 tcgcaaacct tccgagatat ttttcctttc tctttttcag tatttgcggc cgttttaatt
    54001 gatttaggca ttcgacaaat cttccaaatc tcatttgcag aagcaatcat tacggtgctg
    54061 cagccgctat ttacagcagc tgacgggtac ttaggaatcg ctattatctg gggagcgatg
    54121 gcgctgtttt ggtttgtcgg tgttcatgga ccgtcgatcg tggagccggc aattgcggca
    54181 attatctatg ccaatgtgga aaccaatctg caattgttca attccggtga acaagcttct
    54241 aaagtactga cggtgggttt agggaatttt gtaggaacta tgggaggaac tggggcaaca
    54301 ttggttgttc cttttatctt cctactcttt gctaagtcta agcagttgaa ggcagttggg
    54361 aaagcttcct ttattccagt aagtttcgct gtcaacgaac cattgctatt tgcggcaccc
    54421 atgattttga acccttattt ttttattcca tttttgttaa cacctgttgt aaacgtttgc
    54481 ctgttcaagt tttttgtcga tgtattgggc atgaacagct ttatgtacgt tttgccttgg
    54541 gcaactccag caccgatcgg tttggttcta ggaacgggca tgggtatctt agcttttgtt
    54601 ctagcagcgg ttctaatcgt cgttgacttc ctgatctatc taccattttg caaagcatac
    54661 gatgctgtct tagtagagaa agaagcacaa aaagcagagg tgatcgcaac cgaggaaggt
    54721 gcttctgccg atgtacgtga ggaagtacct gtggcagcga gtcaagttat cagcgatgca
    54781 aataaagaaa tcaatgtgtt ggtactttgt gctggcgcag ggacaagtgc gatgttagct
    54841 aatgctttaa aaaaaggtgc atctgaatcg aaagcaccga tcagtgcaat ggcgggtgct
    54901 tatggttctc attacgaaat gctgcaggat tttgatatgg tggtcttagc accgcaagtc
    54961 aattcttact atgatgatat caaagaagac accgatcgct taggaatcaa gctggttgcg
    55021 acaaaaggag cagaatatat cggattaacg agagatcctg aaaaagctgt tcagtttatt
    55081 ctgaaccaat ttcaataaag gaggagccat aatgaaaatg ggaatttggt tgcgtaaagc
    55141 tgttttattt gcagctgtgt taggggcttt gggggctgtt tcaaaaactg ttgaagcatc
    55201 tgccagcgaa tttattcgcg gagcagatat ttcgatcctg tcagatatgg aaaaatctgg
    55261 tgcaaaatat tatgaagacg atatttcaaa agatgcctta gagattttaa aaaataatgg
    55321 tgtcaactat gttcgactgc gtttgtggca ggatccttat aatagtaatg gagaaagtta
    55381 tggagcaggg acaaatgatc tggaaacgac gattgacctt gcaaaacgag caaaaaatct
    55441 gggacttaag gtgctgctgg attttcacta cagtgatttt tgggttgatc ctgggaaaca
    55501 gaatcttcct aaagcatggc aaggtctgac ctttgaagaa atgaatacgg cattgtacga
    55561 ctatacaaag aacgttctgt cggaaatgaa gcagctggac gtttatcctg atatggttca
    55621 gatcggcaat gaactgaata gcggaatgct ctggccttat ggcaagtctt ggggagaagg
    55681 cgggggagaa tttgatcgat tggctgcttt tttgaagtct ggtatccaag ctgttcgaga
    55741 cactcaacct aaaacaactc cagtaatgct ccacttagct gatggtggcg atacaggagc
    55801 atttacttgg tggtttgatg aaatcacgag tcgaggtgtg tcatttgatt taattggagt
    55861 gtcgtattat ccgtattggc acggttcaat gaatgaactt caaaccaata tgaatgcgat
    55921 cagccaacgg tatggtaaag aagtgatcgt agttgaaaca gcttatgggc atacaacagc
    55981 gaatgccgat actatgccaa atgcttttgg tgaagccgaa gcagctgcgg ggggatatga
    56041 accaacaccg acaggacaag cagagtattt gctggatctt gccgatcgga ttcaggcagt
    56101 accgaataat cgcggggcgg gcttcttcta ttgggaacct ctttggtaca acggaaatgt
    56161 gagttgggca acacaagcag gtatgaatta tctcagtgtt caatccacta tgggaaacga
    56221 atgggacaac caagcgatgt ttgattttgc tggaaacgcc ttgccaagct tgcgtgcgtt
    56281 taaacaagca ggggcgcaaa taaatctagt taaaaatgca agtttcgaaa gcgatggacc
    56341 aacgaccagt ccttccaatt ggcaaacctg gtttcaacaa ggaaccgcta agggtacgat
    56401 caaaacggaa gcgggcactt tttttgggga ctacaaacta actttttggc aggatcaagc
    56461 atacgaggca tcagcttatc aaaatgttag tggcataagc aaaggaactt acacattgag
    56521 tgcctatgta atgtctgggg gagagtttga tactaatcag ttatatatca aaaattatgg
    56581 tggagcggaa ctaaatactg cggttgttaa aacaagtgaa tggacaaaaa ttacaatcaa
    56641 agacattcca attacaacag gtgtttgtga aattggggtt tatagcaagg cttcaggaaa
    56701 tgcttggacg aacgttgatt ttgtttcatt ggtgaaacaa tagactgcgg atcgtttcga
    56761 ttcaagatcg gaggttccct tcagccacat cgttagcgta gaaggcagga atcgaacctg
    56821 cacctcgcct ttgccaaaaa taaccagaaa cgatttccag tcatttttac caaaacgagt
    56881 aaagcaccag acttcatcta cactttgtat tatgctaaag agagttcaac gatttcgtaa
    56941 gaagcaatga aaaaattgca aaaagttgtg cagataacat aaagtttgtt taagaaaggg
    57001 taaccttaga attccgtcgt gaatatgatt tgagcactta gatggataga aaaaagttct
    57061 cggaaaacag ttcgtttttc gagaacttct ttttatgata agaatgaaac aaattaactt
    57121 atattatagt ctcttttggt agggaaccat cttattttta atgaaaatca agcattttag
    57181 ctattgaaga atgttgattt atagggattc cccctccata ttatgttggc tctttattcc
    57241 cactctgttt tcaacgtaaa gtgtataaaa cagaatgcga tactatcaag ggagttaaaa
    57301 caatatacag aacagtaaaa cactataaat aaaaataaaa gtgcaataaa agtgcaattt
    57361 ttgcacctaa tcatactctg tttatatatg aaaactagat tctgaatcca atatttggtt
    57421 aacgagaggc gttcgaattt gaacgtcttt tatatattac tttcaaataa atatgaggta
    57481 gaatactatc tattttcttt ttaacccaat tgttataatg gagagtgtaa aatattttgt
    57541 gtaaatagaa aaaaggaagt cccttctgta gaatagagtt accacaacac attcacagaa
    57601 gagaggactt ccctatgaac gattttacta cagaaattct aaagactcta gcgaacaaag
    57661 gcgatttgaa tgaattattc cgtgtccatt tggagaaagc agtcaatacg cttctcaaaa
    57721 cggagttaac ggctttccta gattacgaaa agtatgatcg cattggtttt aacacgggta
    57781 attctcgtaa cggctcctat gaccgtacgg tcaagaccga gtatggggaa cttcatctcc
    57841 agattccgcg cgaccgcaat ggtgagttca agcaacagac tgttcctgct tatagacgga
    57901 cgaatgacac gttagaggag accgtcattc acctcttccg aaaaggtatt accatgtcgg
    57961 aaatcgcaga cttgattgag aaaatgtatg ggcattacta cacgccccaa accatgtcca
    58021 atataacaaa atcatttaca gaagaggtaa cggcgtttaa tgggcgggag cttcatgacc
    58081 gttatgctgc tatttatatg gacgcaacgt atattccgtt aaagcggaaa accgtcgcca
    58141 aggaagctat tcatatcgca gttggcattc gcccggacgg atcaaaggaa gtattgagct
    58201 atgcgattgc accgactgaa tccatcacga tttgggagga aattttattg gaccttcaag
    58261 agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg cttaaagggg atggtaggag
    58321 cgattagtcg gttctatccc aaagctcgtt ttcaacattg ttgtgtacac gtttcccgta
    58381 atatcagtca taaagtgcgt gtcgatgatc gtaaggaagt ctgtgatgat tttaaaatgg
    58441 tgtatcaagc ctcatctaaa gaggtggcgt tggaagcacg tggtactttt gcggaaaaat
    58501 ggaaaaccag ctatccaaaa gtggttgaat cgattctttc gaacgatcac ttgctcactt
    58561 tctatgattt ccccttggcc atacgcaaga gtatttattc tacgaacttg attgaatcct
    58621 ttaataaaca aatcaagaaa tacagccacc gcaaggaaca gttccaaaat gaagagtcga
    58681 tggaacgttt cttagtctcg tcttttgata cttacaacca aaaattccta ggtcgcagtc
    58741 ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat gctaagccaa ctgattgaga
    58801 attagaaaca atctgcagta gggaagaatc atttacacaa aattattgac gctcccttat
    58861 aatgagaact tttactaaca taatttatga taaacttact atataatcat attaattaaa
    58921 tagttaggag atctgcttat gtttatatct actcttcaaa ttcgaaattt tagaaattat
    58981 gaatccgaaa tctttcgatt cgataatgag acaaatacta ttataggtga gaatgattca
    59041 gggaaaacca atgctcttac tgcattgaga atcttattag atgatagtta ttactatagt
    59101 agtaaaacac taaaagaatc agattttttt catggaattc aaaatgggtg gcaagggcat
    59161 tggattatta tctctgcgac atttgaagga atcagcgaag aagaatttga caatgagatt
    59221 tgtgcttcca tttctttaaa ttctgaaagt caaacaatct tagaagaatt gatttcaaat
    59281 gcagataaag gtgtaggtag tatttctcta tttattagac ctaacaaagc tattagaaag
    59341 caattatttg atatttccac tgaattagac tctcatcaat ttaacgaatt tagaaattct
    59401 attcgtctat ccgactatga attttactac acatctaaat caaatctaaa tttctgtatc
    59461 aatgaaaatt atgataaatt ggtaggaaga ctcaatgaca atatagctag taatcctgaa
    59521 gaagacgatg aagctctgtt gggctcaaga attgacatgt cagatatttt caagcatatt
    59581 tcggttgttt atgttgacgc tcttcgagat gttcttagag aaatgaaaaa caatcgaaac
    59641 ccagtcaaaa gaatcatgga aacaattgaa tcaaaaatat catcagataa tgttgagtca
    59701 ttaaaaacaa ttatacagca attaaatgag actattacca gtgttcctga aattaggaaa
    59761 attggcgaaa atattaatcg gcaactaaat agcataattg gttcagtgta ttctcctaat
    59821 cttcttcttt cctcaactat gtcagatgat atgggttctt tagcaaaatt tctttcaatg
    59881 aagccagaac aaaatatcga cttagactta ctagggttag gacatctaaa tatgatttat
    59941 ttagctttga aaatagtcga atttgaagcg tgtcgttcaa gagaactact taatattatg
    60001 ttgattgaag aaccagaagc tcatattcat catcatattc aaaaaactct gttcgaagga
    60061 ttaaatcttc aaaaaaacta tactcagatt ctcatgacca cacattctgt acatttagct
    60121 gaagtgtctg aaatatctcg gatgaatgta ttaaaatcgt ttaatggcaa atctatcgtt
    60181 atgaatccag caaataatct gaactcattt gctaaagaaa agttaaaaaa gaacaatcta
    60241 aatctaataa acgcagtaga gagatattta gatgttaaaa gaaatggatt gcttttttct
    60301 aaaggtgtta tcttagttga aggagatgct gaagaaatac ttattcctca aatagtaaaa
    60361 aaattatttg gtataaactt agacgaactt ggaataggtt tgattaatgt tggtagtact
    60421 tcgtttgaat atatcgctag cttgtttgac gatgatagaa tacagagaaa gtgtgctatt
    60481 gtaactgatt tagatgaaca ggcaatccca tcagaacatc gattgtataa aagcactgca
    60541 caagagaaag gtcaagcaag aaaagaaaaa ttatcctccc tttttaatga aaacaattgg
    60601 gttaattgtt tttatgctaa tacaacattt gaaattgagt ttttagatgt gaataaagaa
    60661 aatattctaa cttatgtcag tccaattata gatgacgcat tcaaagataa aaaaacaata
    60721 actgcttata aagaagaaat acaaagtgaa caatatttag aaagaaattt agcgatgtta
    60781 aaactagcca atcatgttgg taaggggtgg tttgcaattg agttaggtga aaaaattgac
    60841 tttttaatca aaattcctaa gtatattctt aatgctattg cttttgcatg tcaggaaata
    60901 gtagatctct caatttataa aaaaattcta ctccatacaa tcgacttgta cgaggaaact
    60961 acagaattgt cagctttatc agctagttta aaaacaagtg atacagctga tttgaatata
    61021 gcaataattt ctttattaga gtactttggt aaagatgagg atgttgtgta tctaatcaat
    61081 cagatagaaa attacacaaa aatactttat gctgaggttt caaaatgaca aatatcttcg
    61141 aaaaaatcgt aaatgattta aatttagaac aaaaagatgt tgtggaaagt gaagataacc
    61201 tttatgtaaa tgcatgtcct gggagtggga aaacaagggt acttacaagg aaaattgctt
    61261 accaagcaat taaacacgat ggatcactaa aaagaataat tgctatcaca tacactaata
    61321 gagctgctga agaaattaag gaacgcctaa gtttacttag tatagatgat gatattaata
    61381 tttgggtggg gacaatacat cagttttgtc ttgaatttat tatttatcct tttaaaatga
    61441 atctctcaag agtatcaaaa ggatttacaa ttattgatga ttatactcaa agagcttacg
    61501 tcagccaaat taataatatg ctgaatttaa atctcaaaac gtatgaactg aacaaaattg
    61561 atttgtcact ttctcatgat tttaaaatta atgaagttca gtatactcaa gtttctgaaa
    61621 tgtatcacac tatgttggaa aaaaataaag aaattgattt tgatatgatt ttactagtag
    61681 cgtataaaat tttaactgta aacagtaacg ttgcacgtaa tattgctagt aatataaggg
    61741 cgatttatgt ggatgaattt caagatacta gagaattaca atacaatgta atagctaaat
    61801 tacttcaaaa caaccctcaa attcagtcaa tgtttgttgg agatattgac caagcaattt
    61861 atggatcatt agatggtata gctaaatcag ttgaagaact agaaatgcta actagtcaca
    61921 cttttcaatc aaagacgcta catggctgtt acaggtctaa tcagagatta gtagacttct
    61981 actctaattt tcagagttgt ccttacgaga ttgaaagcag agggaataat aaaaacgacc
    62041 gtggttttat aagttatgat tgcaaagtta ctaaggagga tttacccaac ataataaaaa
    62101 atataataaa ggaaaagatt gaaagcggca ttcatgaaaa agatatttgt gtacttgctc
    62161 ctcaacacta tcctctgttt tcactgggtg agtatttaaa gaaagaatta ccgtattgca
    62221 attttgatgc tataaatata tcaccaatta aggtaaataa tcatagttta tttttcaagt
    62281 tagcaatttt gtactttact gaatcaggtg aaaaggttcg aagaagaaag ctaattgcaa
    62341 acgatatttt aattatatta aaaaatgaat ttggaataga agttaaagat tattttattg
    62401 acttagattt attacagcta ataaacagtg tgaaaagaat gtatagtgga gacgacaacg
    62461 gtgttgattt atacaaattt gttaccaaac atatatttgt taatcttaat attgacgaga
    62521 aaaattaccc gtctattttt aagcactatc tattttttat taacgaagta gaagatcgga
    62581 taaaaaataa ttcattatct agtagtgtat ctagttttaa gaagatgttt aaatctcgag
    62641 atggtatcac acttactaca gcacataaag ttaaaggaaa tgagtatgat actgttatcg
    62701 ctattaatct tctaaacggg aaaattcctc attggaatga aatttttaat gtttctgata
    62761 aaggaattag ttcagcgaga aaactactat acgtaatttg ttcaagagca aaaaataatt
    62821 tatttctttt ctcagaggtg ggttatttta cgagtaaagg agacttgctt tatcccactc
    62881 aattattaga taaaatagaa tttgattata atatttaaaa tacctaggta gtcgttgttt
    62941 taaatatagt aggaatcgga cgctcagtac ggagcgtcta tttttttacc caattttgaa
    63001 aggaagtgaa cttatgaaaa taaaaaatca agaatcaaaa ggtcgctctc ccccctttaa
    63061 aaccatcaca caaataagat tcattcaaac ataaaaacag aaaggatagg taaaaatatg
    63121 gaacttaaat atgtcattcc caatatggaa aaaacatttg gacaattaga atatgctggt
    63181 gaagggaaca ttgaacaacg tcgaataaat ggcagaccaa ccattctctc tcgtagttac
    63241 aatttatact caacgattca acgagcagat gatattgttg tcattcttcc ctccgaagct
    63301 ggcgagaaac attttgaatc cgacgaaaaa gtcaaactca tcaacccaca tattaccgca
    63361 gaaggttata aaattggcaa tcgaggattt accaattaca ttatgcacgc agacgatatg
    63421 gtgaaagcat aagaaaggaa aggtgaaaaa atatgagatt agcagaaggt attgtgattg
    63481 aaaaagacca gacatttggc acattgaaat tttccgcctt gcgtcgtgag gttcgtatcc
    63541 agaatgaaga tggtacggtt tccgaagaaa ttaaagaacg tacctatgac ttaaaatcaa
    63601 aaggacaagg acgcatgata caagtcagta tccccgcttc tgttcctttg aaagaatttg
    63661 actacaacgc agaagtggaa ttaatcaatc ctgtggcaga taccgtagcg acggctactt
    63721 ttcgaggggc agacgttgat tggtacgtga aagcagacga tcttgtatta aaaggacaga
    63781 caaaacctca tcagaaatat aaaaaagaag cgtccgaaag tgaaaaatag gtgttgctta
    63841 tgacaaacac accattgatt ttgaaagaaa ttccgaagga tgaagccatt tcttttattc
    63901 ggcaatacca ttattcaaag atactcccta gattgtgtaa atactttcta gggatttttt
    63961 cagaagaaaa attattaggc gttgtggaat taggctgggg tacgcaaccc ctacaaacca
    64021 tacgcaaact ctttcccgac agttcccttc aaaccacaga ttatttagaa attggaaaga
    64081 tgtgttttct tccagaaatg aatcaaacca attattttgg cagtcaagct ctttcagctc
    64141 taattaagtg gttgaaagaa catacggact gtcatttttt atatacactg gcagatggaa
    64201 ttgagggaaa atgtggctat gtctatcaag cgtccaactt cttttactgc gggtatttca
    64261 agacaagtgt ctatcgtgat aagcagtcat gggaaaagat acaccctcga agtgctcgcc
    64321 ttttattaga ggaaaatgct cgctttgaac aggtagagaa aaagcattgg ctttcccagg
    64381 cattttgcga atacaaaggg attgagaaaa tcaatggacg catgtttcgc tatctctatc
    64441 cgttgacaaa agaagcgaaa aaactgttag gacacactct ctatcgacgt cattattatc
    64501 cgaaagaaaa aaatttacgc tttgaaaagc gaatcgctta tcagaaatat gaagccattt
    64561 ctcaaccaac ttttgataaa caggctcgta tctacaatac acaattattt tgataggtgg
    64621 aaaggaggat ttttatgcca ttgcttcatg acagaggaga gcgaatcaaa gccaacgata
    64681 agcacttggt ctatcatttt gtactaattt cattgctggt agtttttcta gtcgtcctac
    64741 tcccttttca ttggagagcg ttacaatcgg tgaactggca agcagttcag ttagatacat
    64801 ttttctatca acttcatacg ccatactttt ggagcagtat tctcactgct ctttttgtat
    64861 gcggacttat cagctggctt tattatcact atcgtataga ttctataaaa cagttagagc
    64921 accgtcaaaa gttagctcgt atgattttag aaaatcattg gtatgaagcc gaagaaactc
    64981 aatcagaggg attctttaaa gatttaccca gcaagaaaac caaacaacga atccgccatt
    65041 ttcctaaaat atattatcga atgaaagatg gactaatcca tattcatgta gaaatcacct
    65101 tagggaaata tcaagatcag cttttgcacc tagaaaacaa actagaaaca ggattgtatt
    65161 gtgaattagt ggaaaaaacg ttgaaagatt cttatgtaga gtatgtccta ctttatgata
    65221 ctattggcaa tcgaattggc attgaagacg tggtagctca agatggacgt gtcaaattga
    65281 tggaatcggt ctattgggag ttcgattctc tccctcacat gttaattgct gggggcactg
    65341 gtagtggaaa atcctatttt attctcacat tgattgaatc tctcctgcat acaaatgcaa
    65401 agctttatat tcttgaccct aaaaatgctg atttggcaga cttaggggcg gtcatgaata
    65461 atgtctatta ccgtaaagaa gatatgttgg catgtatcaa tcaattttac gaagatatga
    65521 ttgatcgcag tgaaaccatg aaacaacacc ctaactataa aacaggagaa aattatgctt
    65581 atcttggttt gtctgccaat tttcttattt ttgatgaata tgtggcaatg atggatatgc
    65641 taggtcgtga aagtaccacc gttattcata aactcaaaca aattgtaatg ttaggacgac
    65701 aagcaggctt ctttttaatt cttgcctgtc aacgtccaga cgcaaaatat tttagtgatg
    65761 ggattcggga caactttaac ttccgtgtgg ctttgggtcg tatgtctgaa atgggctttg
    65821 gtatgatgtt cggaagcgat acacaaaaac aatttttctt aaaacccatc aagggaagag
    65881 gttacgtgga tacaggaaaa agtgtgatta gtgagtttta tacacccctt gtaccaaaaa
    65941 ggtacgattt cttaggagaa ataggaaaag tcattcaaaa aaagcaatct gagcctgtac
    66001 cgcacgaagt gaaaggtaca ggttcagact tgctttgatt ggtgtggcgt tagccacgcc
    66061 aatcctttag ccccctctat ctaacagggg ggcacaaata caactgaaaa caggcaattc
    66121 tcatcaacca atgttgatgg tacaaaggat tgcggaaaat atgaaaggtg taaactcaag
    66181 ggtcatagtt tacatctttt ttagattgga ggtttgcgaa tggaaaacga aaaaatttgg
    66241 attaaggaat tgaaagaaaa gcgactggct tatggcgttt ctcaaaataa actggcggtt
    66301 gcttctcata ttaccagacc ttacctatcg gatattgaaa caggcaaggc agtccctact
    66361 caacaggtga aagaagattt actgaacgct ttggaacgct tcaatcccga taacccattg
    66421 gagatgttat tcgactatgt acggattcga ttccctacca atgatgtggc tcaaattatc
    66481 ggtgaggttt tacgtttgaa tatggattat atgctacacg aagactttgg ctattattct
    66541 tatcccgaac actatcgttt cggtgatatt gttgtcctag tctctcatga tgtaagtaaa
    66601 ggtgttttat tagaactgaa aggaaaaggc tgtcggcaat ttgaaaactt tctattggct
    66661 caacatcgga gctggtatga tttctttcaa gattgcatgg aacataaagg tattttcaaa
    66721 cgcttggatt tagctatcaa cgataaaaca gggattttga atattccaga tttggcaaag
    66781 aaatgtaaac aggaagaatg catttccgtc tttcgaagct ttaaaaatta tcgcagtggt
    66841 gaattggttc accgtgatga aaaaccatac atgggaaaca cactttatat tggctcatta
    66901 aaaagtgaag tctatttctg tatatatgag aaagattatg agcaatacgt gaaaaatgat
    66961 gttcccttag aagacgcaga agtaaagaat cggtttgaaa ttcggttaaa aaatgaccga
    67021 gccacccaag ccatgaaaga tttattggca catcaacagg cggaaaaaac agcctttgaa
    67081 attatcaatc gctatatccg ctttgcagat aaagacgaca ctaaacgtcg gagtgattgg
    67141 aaaactaatg aacgttggga atggtttatc ggtaaaaatc gtggagcatt acgcctaacc
    67201 acacagccag agccttattc ttttgaacga acattgaact ggttacatca tcaagtcgcc
    67261 cctacattaa aaatcgcaag cattttagat gttctcaacg gtacaaccat tatctccacc
    67321 atgattcagg aagccaaact gacagaaaaa catgagaaat taattgaaca acaacatcta
    67381 gccatggagg atttgattac atgagccttt taacaaaaga attaaagaag ttagggtttc
    67441 agtgtggcat tgagtttcaa gcgtatatac aaaatacagg gaaatatacg tctctcatta
    67501 ttgagggaaa acggcaagct ggtgatacca tctataccta tgatttttac aaggtgagat
    67561 tttataataa ttataccaat cgagtaaccg tctatggaga gcatttaacc ccttttcaat
    67621 tattaaggcg tgtaaaaagc tatatctatt atcgagaaaa atatcttaag gaaaggagga
    67681 caatcacatg aattttgggc agaatttgta caactggttc ttggataatg cacagtcatt
    67741 ggtattgtta gcgattgttg tcattggact gtttttaggt tttaaacgag aattttcaaa
    67801 actcattggc ttcttgatta ttgctctaat cgccgtcgga cttgtgttca atgcagctgg
    67861 cgtgaaagat gtgttattaa acttatttaa ccgaatcatt ggtgcttaac agatattttt
    67921 catgataaaa taggaagtat atattttgtt atgaaaggat ttatgaatat gtttacaaat
    67981 gatcaattta aatctctttt aattaaaaca ttagaaagaa agagctcttc aaattattat
    68041 gaaggaggta acgagattgt ttctaaaatg aaaatatcag aggtaacttt agatgagact
    68101 gacgatttta cttattataa aggatataaa aagggctgga atacgctttg cacatatctt
    68161 aaaataagag ttccttttga tgatttagac ttttttgaaa gtcataaaga tttgattacc
    68221 cagacagcaa gctccatata tgataaacaa ggtgataatg tattagtgga tactatttta
    68281 gtaccacttc cagagaatta cgaagtaatt aactttagtc agttgaaaat tagtgatgtg
    68341 gtttcacaag ctattgaaga cgctgagtct tttatgagta atggtgaata tcaacgtgcc
    68401 tttgacagag ttcacactgc ttttcatgga tatttaattg aaatactaaa aaaatatgaa
    68461 attacagttc ctagagacga aaatctatct aaattatata gtaggataca acaattaatt
    68521 gaaaaagaaa ttcaacctac tgaacttgct gatatagtga agacaacgat aaggtcatca
    68581 aatggaatga ttagctctct taatgaagca agaaataggc attcacttgc acaccctaat
    68641 acaaatatta ttggaaagag agaagcaaaa ctaataatag gtatttcaag tacagttaca
    68701 gattatatta gcggatacct tgataagtag cgtcattaac tgattgctac tttttttatg
    68761 actattttta taaaaaggat gtgataagaa ttggaaatga aagtctatgt ggcaaattta
    68821 ggacggtata atgaaggtga attagtcgga gcatggttta ctccaccaat tgatgaggaa
    68881 gaaatggcag aaagaatcgg tttaaatgag gactacgaag aatatgcaat acatgatttt
    68941 gagcttcctt ttgatgtgga cgaatacacg ccgattagtg aaatcaaccg tctatgtgaa
    69001 gccattcaag aaatcgaagg tacacctatt tataatgaac tcaaagaaat tcaaggcatg
    69061 tggtttagta gcttggaaga attactggaa aacaaagagg atattcattg ttatagtgat
    69121 tgtgattcga tggaagatgt cgcccgttat tacgtggaag aaacaggaca gcttggtgaa
    69181 gtcccttcca atttacaaaa ttatattgac taccaatctt tgggacgtga tatggaaata
    69241 gagggtaatt acctagttac ctctcatggg gtatttgaat attgtcagta agtttaacaa
    69301 tccgtgttat aattaaatta ataaatagca cggagaggta taaattatgg taaatgtatt
    69361 tgaaaatctt aaactattaa aactagacat gaaagataaa ggctgggtga tagattcctt
    69421 ttactttcgt tacaaacagc aaaattatat tgtacttgtt aagctatttg aaaaagagga
    69481 aaaagttcca gaatatgctc tgttaaaact agaattttta aaggaaaacg atttttcaga
    69541 tatgctagct gtctatgcta attcggtaaa attatataca gacacgaaaa caataagaga
    69601 atattttggt atcgaataca gtagtaactt gggagatgtt ttatttcaat ttagccaaac
    69661 tcttgctcga tttattccga cagaagttag tgagaagaaa aatgaagacc aaaaagaagc
    69721 catgtgtttc tcattaagtc aaagtgattc agaagatccc agaaaaaaat attgtttttc
    69781 agttagaaga aatcctttaa agggaaatgg tgagctggga aaacgaagtc cttttaatga
    69841 caataaaacg agattgtata gacccagctt atatgaacga ttagggtcag ataccaattt
    69901 aagtttcaga tattcaatga atccagatga tgaagaaact gatgaaggta ttattgcaaa
    69961 atggacaaaa aataaaattg aatagttaaa gcattgaaag agcaatcacc tattttgatt
    70021 gttctttttc tatttttacg gaaaggacgt gaagacatga aaaaaataag aagttacacc
    70081 agtatttggt cagtagaaaa agtgatttat gcgattaatg atttacaact ccctttccca
    70141 attaccttta cgcaaatgac atggtttatc gtttctttgt ttgtcgtcat actctttggc
    70201 agtttaccac cactttctct gattgacgga gcactactca aatacttagg gattcccatt
    70261 gttctcactg ggttcatgtg ccaaaagacc tttgacggaa agaaacccta tggatttcta
    70321 aagtctatca ttacctatgc tctacgccct aaagtaacct acgctggcaa acaaatacga
    70381 ttaaaaaaag aaacaatgaa tgaatcgatt acgattgtaa ggagtgaaca actagatggc
    70441 atatccgatt aaatatatgg aagataatct cgtatttaat catgatggcg aagtattcgc
    70501 ttattatgaa ttaattccct ataattacag ttttttaagt tccgatgaaa aaattcaagt
    70561 tcatgataat ttctgtcaat tgattgccca aaatcgtgat ggcaagattc atgctctgca
    70621 actagcaacc gaatcaagta ttcgagccac acaggaacaa tcgaaaaaag aaatcactgg
    70681 acgattaaaa gagatagctt acgaaaaaat agatgaacag accgaagcgt taatttctat
    70741 gattggtgac aatcaagtag actatcgctt ttttattggt tttaaactct acctcaatga
    70801 acaggaagta tccgtaaaaa atgttgggaa agaaattcaa aaagcatttg cggaattctt
    70861 tcatggtgtg aatcacaaat taatgggcga ttttgtttct atgcctatgg cggaaatcga
    70921 acgcttttct aagatggaac agttgttaga aagtaaatta tccagacgct ttcagattcg
    70981 taaattggag aaagatgatt ttggttatat tattgaacat ctttacgggc aaacaggtgt
    71041 cccttttgat gactacgact accatttacc gaaacgatac gaagataaaa aagtgttggt
    71101 aaagaaatac gatattctca aacccactcg ctgtttaatc gaagaaaatc aacgctattt
    71161 acggattgaa caggaagata ccactactta tgtggcgtat tttacaatta atagtattgt
    71221 tggtgaattg gatttcccta atagtgaaat tttttattac cagcaacagc agtttacttt
    71281 ccccgttgat accagtatga atgttgaaat tgtcgccaat aaaaaagcct tatctaccgt
    71341 tcgcaataaa aagaaagaac tgaaagattt agatagccac gcatgggaaa gcgacaacga
    71401 aaccagtact aatgtggtgg acgctttaga atctgtcgat gaattagaaa gtaatttaga
    71461 ccaaaccaaa gaatctatgt acaaactaag ctatgtggta cgggtatcag caaagaattt
    71521 ggacgagctg aaacaacgtt gtgatgaagt aaaagatttt tatgatgatc tgtctatcaa
    71581 attagtacga ccttttggcg atatgctagg cttgcataat gaatttattc cctcaagcaa
    71641 gcgatatatc aatgactata ttcaatatgt cacgtcggat tttttggctg gtcttggttt
    71701 cggtgctaca caaatgattg gtgaaaattc tggtatttac gtcggttaca atctcgatac
    71761 aggcagaaat atttatctcc aacctagcct tgcaagtcaa ggaattaaag gctctgtaac
    71821 aaatgcatta gcgtccgctt ttattggttc attaggtggc ggaaaatcat tctctaacaa
    71881 tatgctggtc tattattcag tactctttgg cggacaggca accattgttg acccaaaagc
    71941 tgaacgtggt gaatggaaag aaaccttacc tgatattgcc catgaaatca atattgttaa
    72001 tctcacgagt gagaacgaca ataaagggtt acttgaccct tatgtgattt taaaactccc
    72061 gaaagattca gaaagccttg ccattgatat tctcacgttc ttaacaggta tttccagccg
    72121 agatagcgag aagtttcctg tcctgcgtaa agctattcgg agtgtaactc aaagcaaaca
    72181 acgagggttg ttattggtga ttgatgaatt gcgaaaagaa gatacgccca tcagcaatag
    72241 tattgctgac catatcgaaa gctttgtcga ttatgatttt gcccatctgt tattcagtga
    72301 tggcagtgtc acccaatcca ttagcttgga aaaacaactc aatattattc aagtggcaga
    72361 tttggtctta cccgattcca atagctcttt tgaagaatac actaccatgg aattattaag
    72421 tgtggcaatg ctgattgtca ttagtacctt tgctttagac tttatccact ctgaccgttc
    72481 catttttaaa attgttgacc ttgatgaggc ttggagtttt ttacaagtgg cacaaggaaa
    72541 agctctatct atgcgattgg ttcgagcagg acgtgccatg aatgcagggg tctatttcgt
    72601 tactcaaaac gcggacgatt tactagatga aaagatgaag aataatattg gcttgaaatt
    72661 tgcctttcgt tccactgacc tagtggaaat caaaaagacc ttagaatttt ttggtgtgga
    72721 caaggaagat gaaaacaatc agaaacgctt acgtgaatta gaaaatggac aatgtttagt
    72781 ttcagacatc tatggtcgtg taggggttat gcagtttcac ccgatttttg aagagttgct
    72841 tcatgccttt gataccagac cgcctgtgcg aaatgaggtg gaagaatgac acaaaaacaa
    72901 aagaaatttc ttcactatag ttggataacg ctcctttgtg tgggcgttat tttactttta
    72961 ttaggcacgc tgggcaatgc agtacaagcg acaggcttag ttgatgaaac cattgataca
    73021 agcaatgaat actccaagta tggtttgaat caatatcagc ttgattatta tgtggacaat
    73081 agctggggct ggcttccatg gaattggagt gatggcatag gacaatcggt gatgtatgga
    73141 ttgtatgcca ttactaattt tatttggaca atttctcttt atctatcgaa cgccacaggt
    73201 tatttggtga aagaagccta tgccttagat ttcattagtc aaacatccca agcaattggc
    73261 gaaaacatgc agacgattgc aggcattaca ccgaatggct tttccagttc agggttttat
    73321 gtcggtttcc tccttctttt tattctcatt gttggaatct atgttgccta tacaggattg
    73381 gttaagcgag aaacgacgaa agtgatacga gctgttttaa actttgtcgt ggtgtttatt
    73441 ctatcggctt cctttattgc gtatgcaccc gattatattg cgaaaatcaa tgatttttcc
    73501 aaagatgtca gtaccgccag tctgaatatt gggacaaaaa ttgttcttcc taacacggat
    73561 agtcaaggga aagacagtgt ggacatgata cgtgacagct tattctctgt tcaagtaaag
    73621 caaccatggc tattgcttca atatggctcc actgatgtag aagcgttagg ggaagaacgt
    73681 gtggaaagtt tattgtctac cagtccagat acaaacaatg gtgaagatag agaaaatgta
    73741 gtgattgaag aaattgaaga tcatgacaat acctatttaa ccctaccgaa gacgatttcc
    73801 agattaggaa cagtcttctt tttgtttgta ttcaatattg gtatctcgat ttttgttttc
    73861 cttctaacag gaatcatgat tttttcgcaa gtgctattta ttattttcgc tatgtttttg
    73921 ccaatcagtt tcctattaag tatgttgccg agttttgatg gcatgggcag gcatgccatt
    73981 accaagttat ttaatgtgat tatgacaagg gctggtatta ccttgattat taccgttgcc
    74041 tttagtattt ccactatgct ttattcgctg tcagcaagtt ctcccttttt catgattatg
    74101 tttttacaaa ttgtgacctt tgcagggatt tattttaaac tgggcgattt aatgagccta
    74161 ttcactttac aaagcaacga ctctcaaaac atgggcagac aagtgtttcg cagacctcgt
    74221 caaatgatga atcgacaaac cagaagattt caacgcagta tgagtaagat ttttcgtgga
    74281 aaagcagggt caacgaagca aaagacaatg acaacaaagc agaatcaaac caataaagct
    74341 aaccatactc gtcaaaatga acggactaca ccaaagaaag aaacagcact ttccaatgtg
    74401 aaaaagcaac aaggcggtat cgggcaacga ttaggtgaaa agacagccca tgtattggac
    74461 acgaaaaatc gtctagtcaa taaaagaaag caggcaaaac aacagataca ggcgacacca
    74521 acccatgtca aatatgccct tcatcaagga aaagagaatg tcactcataa tgtaacagac
    74581 tttaaacaat ctatggcaga taccaaagct caacgcaaac aacagcgtat gaaccagcag
    74641 gagcaaaaac gaaagaacat tgctcaaaag cgtttggagc tggataaagc aaaagaaaaa
    74701 agacgaaaag aaaacccatc atctcctgtt cagaataacc agcagcatgt tcgcaagcgt
    74761 cagcaaaccg tcacgcctgt tttgtcacaa aacactaaac gtgaaaatca tcagcgacca
    74821 ttgaaaaaag agcaaaaaca aaagacacca ccgaagcaat tcaatcagag gaagctaaat
    74881 aaaagaacgg ataagcgagg acaacaaaca tgaagctaaa aataacagtg atttgtagtg
    74941 tggtcttctc tctcttcttt tttctcttat tattcctagt cattttcttt gcagatgata
    75001 cggacagtgg cgaaaacaac aaggattctt ctattccaca aggcggtgtg accgtttcac
    75061 cagaagtgtt ggcacaccgt ccgttgattg aaaaatatgg taaggaatat gggattgaag
    75121 actatgtttc ttatatctta gccattatgc aagtggaatc gggcggtaca gcagaagatg
    75181 ttatgcaaag ttccgaatct ttgggtttac cgcccaacag tttgagtacc gaagagtcca
    75241 tcaaacaagg ggttaaatat ttcagtgaat tattaaccag tgcagaacaa caaggagtgg
    75301 atatagattc tgttatccaa tcttataatt atggcggtgg ctttttaaat tatgtgagga
    75361 gtcatgggaa aaaatatacc tatgaattag ccgaacagtt ttctaaagag aagtcgggtg
    75421 gtcaaaaagc agactatccc aatcctatag ccatacctgt gaatggtggt tggcgatata
    75481 actatggaaa tcagttttat gttcagttgg tttcacaata cctaaccgac acttccccaa
    75541 cagaatttga tgatgaaacc gtgcaagtca ttatggacga agccttgaaa tacgaagggt
    75601 ttccctatgt ctttggaggg gcgtcgccta ccacctcttt tgattgtagt ggcttaatac
    75661 aatgggtgta tgacaaagct ggcatttctc tccctcgtgt cgcccaagat cagtatgacg
    75721 ctacgcaaga aatctctatg gaagaagccc aagcaggcga ccttatcttc tttcattcaa
    75781 cctataatgc aggcacatat gtgactcatg tggcgatata tttagaggga aatcgctttt
    75841 atcatgcagg cgacccaatc ggttatggcg acttaagcag tcgttattgg caagaccatc
    75901 tgattggggc tagacgtgtt attcataact aagaaaggaa tgaatcaaat gattataaaa
    75961 atcgagcgaa aagaaaagaa ggagaaacca aagaaatcta aaaaaccgag caagcaaaga
    76021 aaagttccga tgattaaagt tggcacgcat aagaaattaa cctttgtttt atgggtttta
    76081 ttgattggta gtgtcggatt tggaatctat aaaaatttta ctgccattga cactcatact
    76141 gtccgtgaaa cagaaatcat taaacagcag attgtcgata ccaaccaagt agaaagtttt
    76201 gtgaaatcct ttgcgaaaga ctacttttcg tggcaacaat cccaagaagc cattgataaa
    76261 cgaaatgaaa aattgaccca ttatttgacg gaagaattgc aggtattaaa tgaagaaatg
    76321 attcgtaagg atattcctac tagttctagt gtgaacgata tacaggtgtg gcaggtttct
    76381 caagtgaatg agaacacctt tgaggtactg ttttctgttg aacaagttat taccgaagac
    76441 aaagacaagg aaactatatc gtctagcttt catgtggttg tccatataga tgaatcagat
    76501 aatatggtta ttatcaaaaa tccgacaatg agtaagaaac ctcaaaaatc ggactaccaa
    76561 cccaaacagc tagaaagtga ccatacagta gatacagaaa cgatggacga aatcactagc
    76621 tttttagaaa cgttcttcca gctctatcct accgccacag aaaaagaatt gacttactat
    76681 gtaagtaatc atgtgttacc tatgattaac aaggaatatg ttttcgagga attggtgaat
    76741 cctatcttta ccagaaaaga taaccaagta atcgtgaatg tcgctgtgaa atatttagat
    76801 caagaaacaa aggcaaccca aatctcgcaa tttgagttga tattagaaaa acaagataat
    76861 tggaagattg taaaataaca aatattggta catgattaca gatactttgt aatcatgtac
    76921 tctttttgat aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa
    76981 gtgactattt aaaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat
    77041 ttgatagcgg gaacaaataa ttagatgtcc ttttttagga gggcttagtt ttttgtaccc
    77101 agtttaagaa tacctttatc atgtgattct aaagtatcca gagaatatct gtatgctttg
    77161 tatacctatg gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta
    77221 tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt
    77281 agctcatgtt gatgc
//
LOCUS       DAFEHS010000005        69095 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFEHS010000005
VERSION     DAFEHS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.005"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(23..391)
                     /locus_tag="LP327_000347"
     CDS             complement(23..391)
                     /locus_tag="LP327_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBL1724496.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(1002..2393)
                     /locus_tag="LP327_000348"
     CDS             complement(1002..2393)
                     /locus_tag="LP327_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBL1724497.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2606..2992)
                     /locus_tag="LP327_000349"
     CDS             complement(2606..2992)
                     /locus_tag="LP327_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBL1724498.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(3048..3671)
                     /locus_tag="LP327_000350"
     CDS             complement(3048..3671)
                     /locus_tag="LP327_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBL1724499.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3735..4403)
                     /locus_tag="LP327_000351"
     CDS             complement(3735..4403)
                     /locus_tag="LP327_000351"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL1724500.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4415..4855)
                     /locus_tag="LP327_000352"
     CDS             complement(4415..4855)
                     /locus_tag="LP327_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724501.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4914..5586)
                     /locus_tag="LP327_000353"
                     /pseudo
     CDS             complement(4914..5586)
                     /locus_tag="LP327_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5771..6169)
                     /locus_tag="LP327_000354"
     CDS             complement(5771..6169)
                     /locus_tag="LP327_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBL1724502.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(6166..6939)
                     /locus_tag="LP327_000355"
     CDS             complement(6166..6939)
                     /locus_tag="LP327_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL1724503.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVLVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6936..7619)
                     /locus_tag="LP327_000356"
     CDS             complement(6936..7619)
                     /locus_tag="LP327_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL1724504.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7786..8673)
                     /locus_tag="LP327_000357"
     CDS             complement(7786..8673)
                     /locus_tag="LP327_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL1724505.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8670..9539)
                     /locus_tag="LP327_000358"
     CDS             complement(8670..9539)
                     /locus_tag="LP327_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1724506.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9676..10962)
                     /locus_tag="LP327_000359"
     CDS             complement(9676..10962)
                     /locus_tag="LP327_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL1724507.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            11235..11813
                     /locus_tag="LP327_000360"
     CDS             11235..11813
                     /locus_tag="LP327_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL1724508.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11813..12742
                     /locus_tag="LP327_000361"
     CDS             11813..12742
                     /locus_tag="LP327_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1724509.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12739..14475
                     /locus_tag="LP327_000362"
     CDS             12739..14475
                     /locus_tag="LP327_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL1724510.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14538..15374)
                     /gene="pheA"
                     /locus_tag="LP327_000363"
     CDS             complement(14538..15374)
                     /gene="pheA"
                     /locus_tag="LP327_000363"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBL1724511.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(15371..15880)
                     /locus_tag="LP327_000364"
     CDS             complement(15371..15880)
                     /locus_tag="LP327_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBL1724512.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15873..17168)
                     /gene="aroA"
                     /locus_tag="LP327_000365"
     CDS             complement(15873..17168)
                     /gene="aroA"
                     /locus_tag="LP327_000365"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL1724513.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(17181..18272)
                     /locus_tag="LP327_000366"
     CDS             complement(17181..18272)
                     /locus_tag="LP327_000366"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBL1724514.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(18289..19455)
                     /gene="aroC"
                     /locus_tag="LP327_000367"
     CDS             complement(18289..19455)
                     /gene="aroC"
                     /locus_tag="LP327_000367"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBL1724515.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19457..20521)
                     /gene="aroB"
                     /locus_tag="LP327_000368"
     CDS             complement(19457..20521)
                     /gene="aroB"
                     /locus_tag="LP327_000368"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBL1724516.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20554..21576)
                     /gene="aroF"
                     /locus_tag="LP327_000369"
     CDS             complement(20554..21576)
                     /gene="aroF"
                     /locus_tag="LP327_000369"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBL1724517.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(22235..23209)
                     /locus_tag="LP327_000370"
     CDS             complement(22235..23209)
                     /locus_tag="LP327_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBL1724518.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(23190..25073)
                     /locus_tag="LP327_000371"
     CDS             complement(23190..25073)
                     /locus_tag="LP327_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBL1724519.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(25091..27316)
                     /locus_tag="LP327_000372"
     CDS             complement(25091..27316)
                     /locus_tag="LP327_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL1724520.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            27431..28465
                     /locus_tag="LP327_000373"
     CDS             27431..28465
                     /locus_tag="LP327_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1724521.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(28532..29545)
                     /locus_tag="LP327_000374"
     CDS             complement(28532..29545)
                     /locus_tag="LP327_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL1724522.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(29623..29811)
                     /locus_tag="LP327_000375"
     CDS             complement(29623..29811)
                     /locus_tag="LP327_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBL1724523.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(30251..30379)
                     /locus_tag="LP327_000376"
     CDS             complement(30251..30379)
                     /locus_tag="LP327_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL1724524.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(30382..31755)
                     /gene="rlmD"
                     /locus_tag="LP327_000377"
     CDS             complement(30382..31755)
                     /gene="rlmD"
                     /locus_tag="LP327_000377"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL1724525.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31866..32921)
                     /locus_tag="LP327_000378"
     CDS             complement(31866..32921)
                     /locus_tag="LP327_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL1724526.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32931..34361)
                     /gene="gatB"
                     /locus_tag="LP327_000379"
     CDS             complement(32931..34361)
                     /gene="gatB"
                     /locus_tag="LP327_000379"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBL1724527.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(34358..35830)
                     /gene="gatA"
                     /locus_tag="LP327_000380"
     CDS             complement(34358..35830)
                     /gene="gatA"
                     /locus_tag="LP327_000380"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBL1724528.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35830..36135)
                     /gene="gatC"
                     /locus_tag="LP327_000381"
     CDS             complement(35830..36135)
                     /gene="gatC"
                     /locus_tag="LP327_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBL1724529.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(36241..38277)
                     /gene="ligA"
                     /locus_tag="LP327_000382"
     CDS             complement(36241..38277)
                     /gene="ligA"
                     /locus_tag="LP327_000382"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBL1724530.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(38294..40528)
                     /gene="pcrA"
                     /locus_tag="LP327_000383"
     CDS             complement(38294..40528)
                     /gene="pcrA"
                     /locus_tag="LP327_000383"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBL1724531.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            40683..42242
                     /locus_tag="LP327_000384"
     CDS             40683..42242
                     /locus_tag="LP327_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBL1724532.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(42353..44284)
                     /locus_tag="LP327_000385"
     CDS             complement(42353..44284)
                     /locus_tag="LP327_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL1724533.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(44302..45216)
                     /gene="pfkB"
                     /locus_tag="LP327_000386"
     CDS             complement(44302..45216)
                     /gene="pfkB"
                     /locus_tag="LP327_000386"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL1724534.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(45213..45965)
                     /locus_tag="LP327_000387"
     CDS             complement(45213..45965)
                     /locus_tag="LP327_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL1724535.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(46125..46334)
                     /locus_tag="LP327_000388"
     CDS             complement(46125..46334)
                     /locus_tag="LP327_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724536.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(46345..47673)
                     /locus_tag="LP327_000389"
     CDS             complement(46345..47673)
                     /locus_tag="LP327_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724537.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            47809..47964
                     /locus_tag="LP327_000390"
     CDS             47809..47964
                     /locus_tag="LP327_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724538.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(48038..48625)
                     /locus_tag="LP327_000391"
     CDS             complement(48038..48625)
                     /locus_tag="LP327_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBL1724539.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(48708..49958)
                     /gene="clpX"
                     /locus_tag="LP327_000392"
     CDS             complement(48708..49958)
                     /gene="clpX"
                     /locus_tag="LP327_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBL1724540.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(50205..51494)
                     /locus_tag="LP327_000393"
     CDS             complement(50205..51494)
                     /locus_tag="LP327_000393"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBL1724541.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(51648..52580)
                     /locus_tag="LP327_000394"
     CDS             complement(51648..52580)
                     /locus_tag="LP327_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724542.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(52737..53396)
                     /locus_tag="LP327_000395"
     CDS             complement(52737..53396)
                     /locus_tag="LP327_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL1724543.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(53399..54424)
                     /gene="sppA"
                     /locus_tag="LP327_000396"
     CDS             complement(53399..54424)
                     /gene="sppA"
                     /locus_tag="LP327_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL1724544.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(54437..54727)
                     /locus_tag="LP327_000397"
     CDS             complement(54437..54727)
                     /locus_tag="LP327_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724545.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            55136..56941
                     /gene="glmS"
                     /locus_tag="LP327_000398"
     CDS             55136..56941
                     /gene="glmS"
                     /locus_tag="LP327_000398"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBL1724546.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(57013..58368)
                     /locus_tag="LP327_000399"
     CDS             complement(57013..58368)
                     /locus_tag="LP327_000399"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBL1724547.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(58392..59561)
                     /locus_tag="LP327_000400"
     CDS             complement(58392..59561)
                     /locus_tag="LP327_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724548.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(59558..60445)
                     /locus_tag="LP327_000401"
     CDS             complement(59558..60445)
                     /locus_tag="LP327_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBL1724549.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     RKNVIQSFLDGMTKGGDRK"
     gene            complement(60634..64326)
                     /gene="nifJ"
                     /locus_tag="LP327_000402"
     CDS             complement(60634..64326)
                     /gene="nifJ"
                     /locus_tag="LP327_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBL1724550.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            64525..65046
                     /locus_tag="LP327_000403"
     CDS             64525..65046
                     /locus_tag="LP327_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL1724551.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(65745..66302)
                     /locus_tag="LP327_000404"
     CDS             complement(65745..66302)
                     /locus_tag="LP327_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724552.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(66481..67092)
                     /locus_tag="LP327_000405"
     CDS             complement(66481..67092)
                     /locus_tag="LP327_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724553.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(67205..67462)
                     /locus_tag="LP327_000406"
     CDS             complement(67205..67462)
                     /locus_tag="LP327_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724554.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(68059..68337)
                     /locus_tag="LP327_000407"
     CDS             complement(68059..68337)
                     /locus_tag="LP327_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724555.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(68371..68622)
                     /locus_tag="LP327_000408"
     CDS             complement(68371..68622)
                     /locus_tag="LP327_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724556.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        19333 a        14926 c        12141 g        22695 t
ORIGIN      
        1 aaaaacaaag tctttatttc tattatttac ctgtaaccgc aaagtagttg ccatcgggat
       61 cagcaaagtt aaagacatat tcttctccaa gctgtaccat atcacctaat gtgactgatt
      121 gcgtctgcat ttttttatat agtccaaaga tatctttact gtaaaacatc aaagatgggg
      181 cactagtagc tgtgccaggt gaatgggact caacaaatgc tcgatcatat aacagaaatg
      241 aggtttgact gtcctcaact cctacttcaa ttataagcgt cccatccatt tcttgacgat
      301 ctttttccgc aaagccaact gctctccaaa acgttgcatt tttttctaca tcgtccacat
      361 aaagcatgat ttttatttca ttcgtaaaca tctgtttcct cctaaacgat ctgttctaac
      421 attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag ctctcaacag
      481 aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt ttttgttatt
      541 tgcgctttca cttttcggaa taaataatgt gtatttatat ggaataaata aaaaaagagg
      601 tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca actcctcggt
      661 cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct ctttgtgact
      721 cagagtgaaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
      781 cggtaataag ctcaaccaaa cgtaaaatat gaggaactat ttttgtttgg ctgttcttat
      841 tacttgatgc agggcagctt tttcacaacc tcttattcac ggtgtttcag aagtagcttc
      901 tcggaaataa gccgaaattt cacaaaaatt tgagaaacta tttttaggaa acttcttctt
      961 atttcttaaa gctgaccact tctatcacaa cctctttttg actatgcatt cacatcgatc
     1021 actttagaat catcattgat gacatcgcga tctaaatctt tgatgcgtgc acttgtcacg
     1081 ataccagcta ccatgctacc gctgacgttt actgctgtac gtgccatatc gattaatggt
     1141 tcaacagaga taactaaccc aacgattgct actggcaagt tcattgcccc taaaacgatc
     1201 agtgaagcaa atgttgcgcc cccaccgact ccagctacac caaaagaact gattgttaca
     1261 attgccacaa gcatcaaaat aaattccaaa ctaaagacat tcactccagc tgctggtgcg
     1321 acgatcgttg ccaacatagc tggataaacg ccagcacagc cgttttgtcc aatagacaaa
     1381 ccaaaacttg ctgagaaatt agctgttgca tcatctactc ctaaagcttt tgtctgagtc
     1441 tcgatattca atggcaaagc tccagcactt gaacgtgaag taaacgcaaa gctcaatacc
     1501 ggaaaggctt ttttgaaata gttgattgga ttgacttttg caccaatcaa aatcagtgag
     1561 tggacaatca aaacagcaag caatgctacg taagaagcaa cgacaaattt acctaaattg
     1621 acgatagctg caaaatcact tgttgccaaa acgtttgtca tcaaagcaaa tactccataa
     1681 ggtgtcaaac gcaaaacaag cgtcacgatc cgcataatga ttttatacag actgtcgact
     1741 aaatctgaaa agaatgctgc ttcttttggc gcttttcttt tcacgcctag ataagcgatt
     1801 ccgacaaatg ccgcgaaaat aacgactcca atcgtacttg ttgcacgact acctgctaaa
     1861 tcagcaaaaa cgtttttagg gataaaggat aaaatctgct gtggaatcga taagttttca
     1921 actgtttcct gacgtgttgc cagttcagag attcgtgctg tttctgcagc tccttgtgtg
     1981 aatttagctc cttggagtcc gaacagcatt gttgaaccaa ttccgatcaa tgctgcaata
     2041 gctgttgttc caagtaaaga agtcaataca gtcgcactaa ttttccctag cttttcagaa
     2101 actttcattt ttgtgaatgc accaatgatc gaaataaaca ctaaaggcat aactagcatt
     2161 tgcaagaatg atacatatcc ttgacctaca atcccgatcc attccattga ttgagtaacg
     2221 actttgtcgt ctacaccaaa gatcaattga agcagaccgc caaagacgat accaactcct
     2281 aatgctgtaa agacgcgtgt tgaaaatttt gtatggcgct tttgcatacg atagaacaca
     2341 tataataatg ccgcaaaaac tagaaccact aaaactgtga tgagtgttgt catttttctc
     2401 ctcctcatat tattaatgac aagtccatcg aaggaagtaa aggttcccga gaatgattct
     2461 tcaaccatcc tcctgaaaaa atttttttca gtgactttta tattttagac gatttttatc
     2521 gaaatgacat tctttttgct tacaattagt aataaaaaaa catacctgtg atagctttaa
     2581 tctatcgcag gtatgttttt actgtctatt cttgtttttc tgattgaaaa ttcaatttta
     2641 ccccaggctg atctaaggcg atctctgtca cctcataaga cattcggcgt acgatatcga
     2701 ataacggtgc tactagctcg tccacttctt cttgtgtgac atcattttga ccttcaattt
     2761 tccgattgag gatatgattg atctgactca cacttccaga taaaacatat gtatcaaaca
     2821 ctaaacggaa ctctaagcgt gctcctacga tgctatgttc tttcggatag ttttcgatcg
     2881 tttcttttaa aggcgagaag cctactttca aatctgtttc tacttcttgg ttcggcaatc
     2941 tcatatcata atgaaatgct tctacaactt ctttttgtct tctgatttcc atataatttt
     3001 atcactcctt tatgaaaatt ataacatatc tcctttgaaa ggggatacta ctttttaatg
     3061 atgctctgca aggtaaggat ttcattcaga tttcctacca cataatctgc cgggatctca
     3121 cccagaaaat gatcgatgtc gtaaagacaa gtagcgactc ctgcattttt ccctgcttcg
     3181 atatctaaac gacgatcgcc gatcatcatc gtgtctgttc tttctaaatg aaaagtatcg
     3241 atcaaataat ttaaagatgc tggatcaggt ttacgaggaa agtcctgatc gatccctact
     3301 acttcctcaa tcagatgtgc caatcgatgt tcttttaaca ggccccatgt tgattctgtc
     3361 aaacggtggg tcaggatgaa atgacgaccg ccgttgtctt ttagcatttc cagtacttgt
     3421 ttcgtttctg gaaatggacg ggaaacttcc gtactttctt tttcatatcg gtgaaataat
     3481 tcattaaact ctctggcatt caagtttgat tcattgatca aataactagt agaatacttt
     3541 ttcattttga agtagatttc ttttttgtcc atactgatac caaaatcttt tagtgcttgc
     3601 catgcaccat ctaccattgc gggatacgta tcaaacaatg taccatcaaa gtcccatata
     3661 taatttttca tgttcctttt gttccattcc tttgcttctt ttttctatct ctcactttta
     3721 gataaaggtg ttctttaaag tatttttttg tccagaattg tcaaatcatc tgctaatgta
     3781 tactggattg gttgtgcatt tgggatatcg atcgtatcca tttgatcttc aggaacatct
     3841 tctaaatatt ttgtcagtgc tcggatgcta ttaccatgac ccacaactag taaatcttgc
     3901 cgatctttta gttttggtgc tagatgatct tgccataagg gaatgatccg attcactgtc
     3961 atttccagac tttctccgta tgggatcaaa tgcggatcca agtgatcata tctgcggtca
     4021 aatctatttt ctgcggttaa cggcggaact tccgtatatc ctctgcgcca tgtttttact
     4081 tgatcttttc cgtatatttc tttcatctta tctttgtttt tacccacaag atccccataa
     4141 tgccgttcat ttagacgcca tgtttttatt ataggaagcc ataactgacc catttcttct
     4201 agtgcgactt gtccggtaag aatcgcacgt ttcaaaacag aggtataaac ggcatcgaat
     4261 tgaagtccgg cttcaagcat tttttgaccg gcaactgctg cttgttcttt tcctttttct
     4321 gttaaggcga catctagcca tcctgtccag taattttcaa aatttgcttc gctttcgcca
     4381 tgacgtaata atactaattt cattcttttc tcccttaaac attcgtttcg aattttatac
     4441 cacatgtttt gcatgtcaca cgtattcgtc cttttccttt aggtgcacgc tgcttttgat
     4501 gacaattcgg gcactcaaaa aaagtataac tttttcgatc cattttgact tgctttttgt
     4561 tttttatctg atgatatttt tctttgactc tatcaagata tttcaaataa cgttgattct
     4621 catttagtct ggggtagatt ttttttgata ggaaacgaaa tatcatccat ccgaaaaaga
     4681 cgatgaacaa caattgtccg attctatagg gcaaccaacg acttagcaaa ctaaagacaa
     4741 gcgcagcgat caatagaccg cgattcaact gatcaaattt cgcatatctt ccctgcataa
     4801 gtttgtgata gttctgctga aatcgtatta gcctttctaa ccatgtttgc atcatattat
     4861 ctcctccatc atctaatatt tattatgaca aaaaaatcca aaaagaaaaa gccttaattg
     4921 gccttttcca ttgcaacttg gtgaaggaca ttccgatttc tggtaaagtc aatcacaatc
     4981 actttctttt tatcttctct gttgactact cgaaatgtgt ccgtcaatgg aagaaacagc
     5041 ttcttagatg gtagctgttg catacgtata aaacgatgga tcatcgtaat caattgagga
     5101 aattgaatag acgtgtgaag cagacctgga tattttttca acagctgtgt cgttttgatc
     5161 agattccgtt ttttactgat cacttttaaa aagcttttta gaatcttttg ctgacgtcta
     5221 aaaatagaaa aagaattttc gtcatctatt tgataacgca tttgtttttg cagttctgct
     5281 gcagtcagat ggatcttccc atcgtttgta tcgatcatta aaccttctgg aaacagttca
     5341 accactgctc gatcccaaga ttcaagagaa agctcgatat aatcttttac tggtaattcc
     5401 aattctacct taaaaaagtg caaaacagcc gacagccctt ctgcttgata gatttctcct
     5461 aatgttagcc cctctagatg gaactctggt gaaaaaatgc agcaatgttt tattttcctg
     5521 caattgaaca tatagaaatg tagatcgctc aatcagagta tcttttaaaa caactaaaaa
     5581 attcattttt ctcccccttt tttcatcatt tttaaaatag catattttga aaagaaagtc
     5641 tgatgcttag agtagaattt cataaaaatt tactatttca taagtattca cgagaaaaat
     5701 acaaacaaaa aagcggaaat aacgctcatc cgccttcttg tttgtgttta tttcctcttc
     5761 aaacgcttgt ctatgattca gacttttctc tatcgttatt ttcatctaaa agttcttgca
     5821 ttccgataat ttctcgatat tgatgaatct tagtgatacc aatgactttg tctacatcaa
     5881 tactaaaaca aataccatta cctggaatat cgatgttgat gccttgacca atcgcatcca
     5941 ttactttatt cgtcaaacta tcgggtacta atgtcaacac aatatctttt tctggcgaaa
     6001 tgtccaaacc taaaaatttc cctgaatcat gtacaccaga acctcttcca tgtaagatcg
     6061 ttccgcctgc tgcacctgct tcttttgaaa agtcgataac atcttcgccc attcctccgt
     6121 caacgatcgt cacaatcatg tgaagattca atgctacaag atctttcatt tgctttcccc
     6181 caatttttgc aatctttctt tttcgattct gtctttttca tcttttcgtt tataaatata
     6241 tcctagtact aaagtggata atattggtgt caatgcaatc attgcaacca caccaaaccc
     6301 gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt gccacgctca ttgataaaat
     6361 aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta tcaaaagcca ttgctaaaaa
     6421 gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg tatcctggga tcaagaaata
     6481 ataaagagag aatccgacga gcaaacgcca catagacaat cctactgctg cgccgatccc
     6541 aattgaaatt accatcagca tgattttcga tcggattctc ccctcgctca attcttcaac
     6601 ctgtttgatc attacatgaa tcgccggctc agcaaaccct actaagaacc ccattaaaaa
     6661 gccaagggga accagtatat aattgtaagg caatcccgca atagcactgc ctaaatgctg
     6721 tcctaccagt acataagcca tattgactcc gtgcaggaaa aggatcaacc caatagtcgt
     6781 tatcaagaaa cctttcataa tgtcaaagaa acgctttttg cgcatacgaa aactaatgat
     6841 attcattaaa acaaatagga ttacgatagg tgccactgcc aaaatgactt ccatggtaac
     6901 gatttcgatt ccatctatca cttgctgcca gaaactcatt ggaagatcac ccccaaaatc
     6961 atcacggcaa gaatcggccc taacgaagca ataccgacca ttccaaaact gtcattttca
     7021 tttttctttt gtttgatcat actagtcatc cctccggcaa gggccaagat aaatggaaca
     7081 gttactggac cagtagtcac tccccccgaa tcaaaagcaa tcggcataaa ttcggaactg
     7141 gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag ccatcaattg ataataggat
     7201 agcttgaata cgacccgaag cagcgcaaag gctagaaata ctccagtgcc gacgctgacg
     7261 atcccaatca gcaacactct gccaatctct ccgtcagata tatcatatac ttgttgtccc
     7321 aaaacttgca cggaaggttc agcaatcgtg atcacgatcc cgatagcaaa tcccaaactg
     7381 accaaaacgg tcaaactttt ctttttgatc atatatttcc ctactagatc tccgacttcc
     7441 atcatagaat aatctgctcc aaacaaaaag agcgtcattc cgatcatcat gattgccgca
     7501 ccaaccagaa atgaagcgag gtcttctcct tctaacggtg caaaaacaaa cgttaaaatg
     7561 acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt ttttagcatg
     7621 tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg agcacaaact
     7681 taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa aagtatcttt
     7741 ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt ttacagagcc
     7801 gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt
     7861 agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag cacccatagc
     7921 agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta agggccataa
     7981 ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg tatttttagc
     8041 cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta atcgagcggc
     8101 ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga tcaaaaaagc
     8161 agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt
     8221 caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa ccatcatcga
     8281 tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct caaatccata
     8341 tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga tcatcgctga
     8401 attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa aattgatgat
     8461 caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat tcgtcattcc
     8521 caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta atgctgtata
     8581 ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga cgttggggcg
     8641 ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac tttcgtttgg
     8701 attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc cgcatatcca
     8761 aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt ggtcgttgca
     8821 tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa tgtcccgatc
     8881 gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt cactttgata
     8941 aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc cgaatcaata
     9001 ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca agtgatcgaa
     9061 agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc
     9121 ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag acttttcatg
     9181 atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt tcgaaacaac
     9241 cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc tagaaacaac
     9301 atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt tttaaaagtt
     9361 gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt gacaggggga
     9421 ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat gattgatatc
     9481 aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt
     9541 cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag acaatatctg
     9601 taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca
     9661 cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg tatctgctag
     9721 taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta cgactgcttc
     9781 aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa
     9841 atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag cttcttgttc
     9901 attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc ccatcagttc
     9961 ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc cagctactcc
    10021 tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt gcagattttc
    10081 tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg ctccttggat
    10141 cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt gtatcgcagt
    10201 tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa gagtggcaaa
    10261 caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct ccccatcttc
    10321 agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa ggtcagaagg
    10381 attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta cttgaatata
    10441 ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg ccatcagatc
    10501 cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata ttttatctcc
    10561 tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa tagcagacaa
    10621 attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat cttcaatcaa
    10681 gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca gtttctgaac
    10741 gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct cataaacttt
    10801 ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga cagtgacttt
    10861 ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac ctgatgctgc
    10921 cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc gctccttttc
    10981 catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa acggtttctt
    11041 tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa atcaactatg
    11101 acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt tctttaaaat
    11161 aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag actattctta
    11221 aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag acgattttag
    11281 atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata gggacttttt
    11341 ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc gtggtcacag
    11401 atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa cagcaacaac
    11461 ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt aaagattctt
    11521 ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat ttttttctaa
    11581 attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc ccaatcacca
    11641 atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa actcgtccat
    11701 tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg aaaaacggcc
    11761 attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat tgatggatag
    11821 cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg gctatgtaat
    11881 caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc aagaagtttt
    11941 tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca atgaaatatt
    12001 ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa agaaccttgc
    12061 ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa gtagttattt
    12121 aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga atttcttcac
    12181 gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa tcaatcgttt
    12241 ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac tttctttgct
    12301 caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt tttctattct
    12361 tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa aagacaattt
    12421 cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag aaatcaattt
    12481 acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca tctttactga
    12541 aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta aagccaagct
    12601 ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg gctataccga
    12661 actgggttat ttctccagag tttttaaaga agagacagga atgacacctt ctcaataccg
    12721 taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt tgccaaactt
    12781 ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt gtttatttat
    12841 catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt ggtactaaaa
    12901 aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt agccgctttt
    12961 gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca tatggaacag
    13021 cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat ccatccaatg
    13081 atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg aaacgacatg
    13141 ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag agtgaatgaa
    13201 cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg cgataagaaa
    13261 gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt ttacggatat
    13321 gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt agtaaatcct
    13381 gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc aaatgaagaa
    13441 aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga aacaacgatt
    13501 tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac attactcagt
    13561 caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat tattttgtat
    13621 aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga tcgacacaca
    13681 aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg cttaaaagct
    13741 actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat caatcaattg
    13801 ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa gaaacgttcg
    13861 cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa tacgctgact
    13921 agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg aatcgacagt
    13981 ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc agtcaaggaa
    14041 gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg cgaaagaatc
    14101 tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa tatgatcctt
    14161 caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca aggattcatc
    14221 acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga taacggcgat
    14281 ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat cggcatcagc
    14341 catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac gatccaatca
    14401 gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa aaatcaatca
    14461 gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt aaataaatca
    14521 atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg ataacagccc
    14581 aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc tactaatgtc
    14641 attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct agttttcaat
    14701 ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc cagcatttta
    14761 tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt tactggagct
    14821 gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg gttcaactca
    14881 ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt ttctaatgaa
    14941 gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc agcagttgtt
    15001 gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg ttgtgattgt
    15061 gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt gcctaaaagc
    15121 tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg tttggaaaga
    15181 agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac gacagccaga
    15241 tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata cgcagtcaat
    15301 tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc tggccctaga
    15361 tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc ttttctggtg
    15421 tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa agtcgttgtg
    15481 ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga atagacgttt
    15541 catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa agaacaatcg
    15601 gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca ccagtcgcga
    15661 taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt cgaaagcttt
    15721 tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc tcaatctttg
    15781 tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg attgttgttt
    15841 ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc tcctgacaca
    15901 agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc agctccttct
    15961 agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc gattcgatga
    16021 tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat gatcagaccg
    16081 tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac cgcatcaatt
    16141 ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat tccttctgct
    16201 tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat gatttctcca
    16261 ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga ttggttctgt
    16321 tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc taagatacca
    16381 gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg aacaagaagt
    16441 cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat ctgttgacca
    16501 gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat ccttccgcca
    16561 aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac gattacggat
    16621 gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc actggctaca
    16681 ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat tggtagatac
    16741 tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc catcactcgc
    16801 cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa aggctgtcca
    16861 gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc aattgattcg
    16921 gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc ttcctcttct
    16981 tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc ttctgcgcgc
    17041 aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat gctgcgatgg
    17101 gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat ttgttgtaat
    17161 aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt agacataatg
    17221 attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag tattcctaaa
    17281 atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc ttttccgatc
    17341 agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa taaatcaaaa
    17401 aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc ttttgcccga
    17461 ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc tatttgtgtc
    17521 atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct caacaaaata
    17581 tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa gcctcctgct
    17641 gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt gactaagcca
    17701 gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg ttctcttgct
    17761 gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt ttcaacatct
    17821 tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag tttagcatct
    17881 gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac aaaccgaaga
    17941 tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc cgtaatgatg
    18001 acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt tgtacggacg
    18061 ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc acttgcaacg
    18121 taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact atcccaacca
    18181 actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc accaatcagt
    18241 ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt agtaatgttt
    18301 caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat cacttgggaa
    18361 ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa cgacgctagc
    18421 tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt cttttgtatc
    18481 gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga ccccgcgaat
    18541 gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat tggtacgtcg
    18601 gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc ctggtaatga
    18661 accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac taaccacagc
    18721 ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta atccagctgg
    18781 aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt tctttgtctg
    18841 atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta cttctgacgc
    18901 ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg cttggattcc
    18961 accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa ttttcttggc
    19021 aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt caagcacatt
    19081 ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc ctggacgcgg
    19141 acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag acatgacagt
    19201 tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac caattgtttt
    19261 gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca tccttcctcc
    19321 tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt ctgacagagg
    19381 taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg attctcccgc
    19441 agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct gtgggaacag
    19501 gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg cttcctcttg
    19561 ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga tcgtacgtaa
    19621 tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca gttggagaca
    19681 agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact ccttctccat
    19741 gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta tgaccaaaat
    19801 ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt cgtttgattt
    19861 ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg tctttcagct
    19921 cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg atttcagcga
    19981 ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca atcaatacac
    20041 cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg actgctgtct
    20101 tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga acgaaatgga
    20161 tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt cctccaccaa
    20221 gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg tataactcag
    20281 ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg actggttcaa
    20341 aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca acgtttgtat
    20401 cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat ccagcctgtt
    20461 ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt acagtaagca
    20521 ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa tcttttccat
    20581 tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt cattcaacga
    20641 ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga tcatgccatc
    20701 tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa ttcctgtacc
    20761 atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg ggacagcact
    20821 taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt cgataaaaat
    20881 gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt cattgatcgt
    20941 accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag cagatagcag
    21001 tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact cagccaccat
    21061 atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt catcagccgc
    21121 ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg gagaagtacg
    21181 tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca ttcgcgcaca
    21241 ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca cgaaactgcc
    21301 gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga attctcgaga
    21361 agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat agcgtgtaaa
    21421 tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc gttctgttcc
    21481 ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt tttttatttg
    21541 ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt tcttttgttt
    21601 acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat taattcctac
    21661 taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta tgacccagat
    21721 aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat cttcaacgag
    21781 ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct ctacgtggct
    21841 gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg tttgtaccca
    21901 cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc agttttgtaa
    21961 gttttctaga tttcataaaa aatcgctcca aactgatgta agaattgtac ctagtataga
    22021 cataaaaaaa tcgactgtca atcattttta tgaaactttt tttaattttc tgatagttaa
    22081 ctttgttttt cagaaaaata acaattggtt ttattgaaga aaatagtcat gaacaaaagt
    22141 atctgagaac ttttttactc tcttgttcat gacatataga ataaagcagc tttgtcttgt
    22201 aaactccgtt tttcaaataa taatctaaag attgctactg aataataaag ccgatttcat
    22261 ggtcctcttc gctgctgatt cggtaaggtg actcaacatc cgctccccag ctattgatac
    22321 ccccgactcc tcttactttt gcaaaaatcg taagaaccgt tcgtctttct ggcggtagtt
    22381 cttctatatg tgttgcctgt tccagttctt ctgctttgaa tggtaaagcg gaaaaagcaa
    22441 atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc ttgatcttta tccgtattat
    22501 ccaacgtttg atttcttgtt agttctaccc atttcgtttc catatgcatg ccgcattctt
    22561 ggggaacaag gtatggggaa acggggagtc ctgccacttt ataaatacct tcgacaccac
    22621 cagccatccg atccggatat gtttcccctg ataggccttc atactcaaaa aattttgctt
    22681 tcgttggcat aatgaacctt aaacccaatg cgggtaattc cggtaatcct ttttgtccat
    22741 aataatgcat attgacaagc attttaccct ctccagcgat ttggtaggaa attgtcactt
    22801 gggtttttgg tactgtgttg atttccagtg taaaaatgat ttcaaccgtt tcagcgtatt
    22861 cattacttga atattgatta tttactggag caatcggcaa atcgatcttt ttattgtcca
    22921 ccagtacttc tatttctttg acttttggat acaatccggc tcctaaccac ataccggatt
    22981 taaatccaaa tccgctacct ctgtcattat cggtcgttgc tcgccaaaaa actggaagtg
    23041 tttcccggta tagccattct tttccgtttt tattgagcga ttccagtccg cctcttgtat
    23101 aagaaaaaat ataatgcgta ttccccgatt tgacacctaa tgtacagtcg ccgaagacga
    23161 ttgcaacttt ttccgtatcc atatgacttt tatttactgt atttttcata ataatatctt
    23221 acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa cgatcccatt tcctgagaat
    23281 tcatagtcac ttggtttatc gtcaaagtca ccgccatagc gcatcacttc tctgcctgtg
    23341 attggatcag tcacttggat cgcctgatct ttaaaatccc atatatagcc tccttggtac
    23401 atttcataac gatcaagcaa cgtcatgtaa gaatccattc cacctaacga attccccatg
    23461 tcatgcatat attcacataa gatgaacggt ttttccgctt tattttcaag gtagtcaatg
    23521 atctcttctg gtggcgcata catgcagctt tttacatctg aaatcgaatc ttcgaatgct
    23581 ggatttggaa aattcccttc ataatggaca agtcgatcgg ggtctacttt tttgaagtaa
    23641 tcgttcattt gtcgaatgtt ttcttccgca taagattcat ttccaagaga ccaaaaaaga
    23701 atacttggat ggttttttaa tgtttcgaaa ttcgtttttg ctcgatcaag aacagccgcc
    23761 tgccactgag gtactgcacc tggtacgttg taagaaggct caaatgcgcc cattttttgc
    23821 catgagccat gggattcgag gttcgtttcc gccatcacat ggattcctgc ttggtcgcat
    23881 aaataatacc aagtgatctg gttggggtaa tgagatgtcc gtacgccgtt gatctttgca
    23941 ttttttatga tagccaaatc tttttccata tcttctatag tgatggcgcg tccttttttc
    24001 ggatgccact catgtcgatt taccccgttg ataatcatcc gttttccatt gaagtagatg
    24061 attttatctt tgatttcgat tttacgaaaa ccgaaggaat agtcaatata ctccgttact
    24121 cttttttctt cgtctatcgt ctccaattca agcttatata aattgggctg aaaatgcccc
    24181 cacagtgcca cattttcaaa tccatcgatg cgaatcgtca tttgctcttt taacggatat
    24241 tgctgttgat agatttcctt cccatcagga tctagtatcg ctacattcag ctgactgttt
    24301 ttggctactt gctcaaaaga caaatctact tcaagtttgc cattttgaca tttgtcatct
    24361 aagcgaggtt tgatccaaag atcttctaaa tggttttgtc ctcgagcaac taagaagaca
    24421 tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt ccaagaatgc agctgtactt
    24481 cttttgtaaa cctccacagc tagaatattt tcttcttcca aataaggtgt taaatcaaat
    24541 tcagacggag tgaacgaatc ttcggcatac ccgataaaat gaccattcaa ccaaataaac
    24601 attgcttgtt ctacaccagc aaaatgaatc gagatttgtt ttccctttag accttcttct
    24661 aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt tagcagctga aaagatccct
    24721 tccgcctctt tctcagggct tgtcgtgaaa gggggacggc gaaatacatg tccctcccac
    24781 ggatacatcg tattgatgta atggatcttg tcataatttg ctaattcaat atgactagga
    24841 acagtgattt catcaaaaga acaggcatca aaatcttttt tataaaatgt ttttggtcgt
    24901 accgaaggat ttggcgaata acaaaaacgc catgatccat ttaaagactg tttaagggag
    24961 gatgtggttt ctacttcttc ccttgttgca taacaaacat gatcgctatg tgcatttgat
    25021 tggttcactc taaaaacttg tggatcatct aaccatttta tatttgcttt catacttttt
    25081 tccttctctc tcataacttt ttgaaatgaa gcagttctgt ttggaaatca ccttgaaaat
    25141 cggggaagta aagtccaact gtcatcaatt cggcccccga aaatatccgc ttcgaggtct
    25201 gttcctgata aagtgcctca tcatctaacc ccatcagata tacttcatgg aacgctggtt
    25261 gagctgaagc tagtatcctc cctaaaaaca caatagcttc atcttgttta ggagacacaa
    25321 acatccatgc ggcaatattt tcttcaaaag gactcgccaa acgataataa tggccatact
    25381 ggatcaccgg acgaatcgtt tgataggata taatttggtc ttttacttgc tttttttctt
    25441 caggagacag ctttgtcaaa tccagttcat aaccaaaaac agcgctcata gctgtttctc
    25501 ctcttgtttt caacgaagta gatctgtcgg tctgatgatt tggtacagct gatacatgag
    25561 ctgtgatgga cgaaatcgga taagcaaggc ttgttccata ttggattttc atacgctcca
    25621 ctgcatccgt gttatcactt gtccatgact gcggcatata ataaataaac ccggcatcaa
    25681 accgtccacc tccaccagaa cagccttccc ataagatttc cggataatca gttgtcaaat
    25741 gttcaagtag ttcataaagc ccgagcatat aacgatgcgc tacttctcct tgtctctgtg
    25801 ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt catatcccat ttgatgtagt
    25861 caagaggaat cgtatctaaa atctttctca tttgtttctc aatcgtctgc cgaacatcta
    25921 accttgtaaa atccagtaca tgttgtgatc tggaagcaga aggcattcta cctggctcct
    25981 gcataagaaa atcaggatgt gttcgataca gttcagaatc aaccgagatc atttcaggtt
    26041 caaaccataa accaaatttc aatcctttgc tatgagcata atctgctatt tcacgtaatc
    26101 catttgtcaa cttgccttca tactcgaacc agtctcctaa tgaagaattg tccgcatctc
    26161 tttttccaaa ccagccatca tctaatacga acatttcgat gcctagctcc tttgcttcat
    26221 caatgatcgc cttgattttt tcactattga agtcaaagta agtggcttcc cagttattga
    26281 ccagaatcgg ccgttcagca aattgatgac gacctcttgc gattctttca cgtagcaact
    26341 catggtggat ctgactcatt ttgttcaatc cctgacttga gtaagaaagg atcatttcag
    26401 gtgtttgaaa agactggcct gcattcaatt cccacgaaaa acgatggctg ttgatcccac
    26461 cgacgactct caattgattg atctgatctt tttcaagttc gaaagaatgg ttgccggaat
    26521 aaacaaattg aagtccgatc gcttctccag tattttctgt cgtatgatga tgaactaaag
    26581 caataaagtt attcatatgg tgactgctcg ttcctcgacg gctttcaaat acttttgttc
    26641 cagataaaac agattgccgt gaaatctgtc tttcattgac atgtgctcct ggcaaagcaa
    26701 tgacttcatc aaatcgccta gtatgggcaa agtctagctg gaaactagcc gctttttcta
    26761 gtttgattgt ttctccggtt tcatttctta tctttacaga tcgtgtgatc actgctctat
    26821 cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt ttctctatcc ttaagtgaaa
    26881 tcaccaacgt ttgtgcctct gacttgtcca tcacataagt ttgaggcaag ccttcgatct
    26941 ctggcttccc atctgtaata taatggctgt catatctcag atccagtaag tctgatccat
    27001 tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat gctgccatga gaagagtatt
    27061 cttgcggtag tgtatctttc gacagagacc ggtcaggatt taagggaaca tttcctgaaa
    27121 atccacgatc tcttcgggga taatttttat tgtctttgta ctgcttgact ggtttgccaa
    27181 aataaatatg cgccaataca ttgctttctt ctatctttat cagataagaa agaaatgtat
    27241 ttcgtaaatg gaagactcgg ctcttttcat caaataagat gaaattttta tctgtttttc
    27301 tatgcgtaat atccatcatt tcactcccaa caaaacggaa accgtttact tttttttata
    27361 attcctgtat actgtttact aaacaatttt gtcaataaaa attttttatt tttttgagga
    27421 gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag gcaaatgtct ctcctgctac
    27481 agtttcccgt gtgttgaata acgatcagac gatccatgtg gctcagacta ctcggcaacg
    27541 gatagtagag gcagctgctg cgttggatta tgacatcagt actcgtaaat atatcaaaaa
    27601 gagaatgcct tcgattggtg tgatctctac gatcggcaaa gcgagcgaag aacaggatat
    27661 ctattacaaa catttgcgag tagggatgga agaagaagct cgacgtctgc atttagggat
    27721 gaatcgaatc tataatctat ctgataatcc taaacaatgg gaagatctag atcagttagg
    27781 tgcggtgatc gttgttggga ctgttacgaa agaatcgatt accgccttgt taaaacaaaa
    27841 catgcaaata gtcgtcgtgg ataaccctga catccagcaa gaggtcgata tggtgtatgc
    27901 agatcttgaa cggatgacta aaaccgtact ggaattgttc ttagaaaatg gtcataaaag
    27961 aatagcctat atcgggggat ataaagtcga tatggatgaa ttcggtggga agcagatttc
    28021 tataaatgaa aagaggatgc gtgcctatca gcattttatg atcgatcatc acttagaaaa
    28081 atatatgtct tatcatctag gtggctggac agaaaaacaa ggtgaggtac ttgcgactaa
    28141 tctattagaa aatgaatccc ctcttcctac tgccattctt gtcgggagcg atccaatggc
    28201 aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa attggagaag agattgttgt
    28261 agcaagcttt gataatatcg agacaatcgc aaacctctcc cctggcttga ccagtgttca
    28321 aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg gcattggagc gaattgacgg
    28381 tatacgggac gaaccaatca ttttgactta tcctgcacga ttgattgtta gggaaagttt
    28441 tatcccaaga acaaaaaggt attgaaagag aggttgtgaa aagccgcttc gtttcttcat
    28501 actcaaaacg aaacggcttt ttcacaacct cttacttata cttattcaca aaatccaatg
    28561 tctgctggta acaattttgc gattctttta gcgtgtaatc aaattggtat tcatgggtaa
    28621 tctctttttt tgtatctgta taaaacaagg attgcacggg tacctttaat tctgtcaacc
    28681 gattttctaa tgcttgacct tgctcctgga atgagtatgc atttccgtca gttatatacg
    28741 ttggcggaaa ttccttagaa acatggtcag ccacactagc ttctttcaat tgctcactcg
    28801 ttttccaatc ttttgttcca atcaacgacc acgcaacggt tttgacgaaa aacttcataa
    28861 atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc aacaggtccg caataagaaa
    28921 tggttccttt cagactatct tttggaagta gctgtttgat tttcatttgt ttagcatacg
    28981 catcattcgt ttgtatagct gcatattgca atgcgatttg tgaacctgca ctatctccac
    29041 caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt ttcttcagct aataaatctt
    29101 tgatcagttc attcacttga agaacttgat tagggtattc tgaatcaggc gctaattcat
    29161 aattcatcgc aaccactgcg atgttggcgt ctgaaactaa ttttgtcgca aattctttga
    29221 cacccgattt atctccgccg acgaatcctc caccatgtac ccaaaataga acaggaacag
    29281 gccccttgct gttttttgga tagtagatgt cataagtatt ctctttatac ttggactcat
    29341 acgtcaaatc tgtttttttc gccacgtttt tttctgcttg atcgaacgtt tttttatcag
    29401 tgatttgaac agtatttgaa aacatatggt tgatcaagaa tgctccaggt ctgggggaaa
    29461 gctggaaggc aagaaagatt gcaaacgcga tcccaagaat gattccgaag aaccatccga
    29521 taaatttaag aattttcttt ttcataaggc tccctcagta gttagtatta agcattctga
    29581 ttataccata gtgttatttc ctatcaagct ctcctagatg atttaatgat tttccccaaa
    29641 cttcttctgc aatgcttttg acaaaagaaa ttttttccat tgttcttcaa acaaaacatt
    29701 ttcttcttct gttgaagcaa atccacattg aggacttaag cacaaacgat ccaaaggcag
    29761 atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg tgctcttcca tttcgccatt
    29821 tttcgttaca gtcaaaatct aagacaacta atttatcttc agagactttc tctaaaagaa
    29881 caaaactccc agcacgttca ctatcgtatt ctaaataata ggcacggaca ttttcttgag
    29941 taaaataatg ggtctgctac gtcgtcatcc cccctgatgc cgccaaaatc gaataataat
    30001 ttccgcgtca aacatgtata tttagtgttg aatcatttga gaatatgaaa ataaaaatct
    30061 ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat ctgagacaga atgacgttca
    30121 ttctgaccca gatttgattt tgcacaactc aacagtcctt attggcaaat cgccaaaata
    30181 atgtagacta actaaaaaga ctaagaaaca ataacaaaag ttaacacttc ttagtctttc
    30241 tttagcacgt ctatttatta aagattttag ggccttttct catgttttta cgatcattct
    30301 taggtgtttc catttgtttg gatcggcctc caccttgttt ctttttgatc aattctttca
    30361 ttttttcttt ttggtccatt tttattctcc tttaacgaat gaagcgacta attcgatatg
    30421 atgggtttgc gggaaaagat ctacaggctg aactttcttc acggcataac ctgcttctgc
    30481 aaataactta gcatctcttg caaaagttgc cggattacat gagatataga cgattcgttc
    30541 tggtgacatt tcgattgctg attcaataaa atgatgatct aatccttttc gtggagggtc
    30601 aactacgatc acagatggtt tgatcccttt ttcttgccat tttttcataa ctttttctgc
    30661 ttttccgact tcataatgaa cattttcgat tttattattt aaagcattga actgtgcatc
    30721 ttcaattgct tctggaataa cttccatccc atacacctta tccactctgt ctgctaaaga
    30781 caagccaatc gtacctattc ctgaataagc gtcgatcaca atgtcttttt tatctaactc
    30841 ggcaaactcg atggctgttt ggtacagata ctctgcttgt tgagtattta cttgataaaa
    30901 tgactttgct gagattcgat aggtttttcc taatagctga tcttcgatat agtctctgcc
    30961 atataaaact ttttctttgt ctcctaaaat cacatttgtt ttttcttcat tgatattttg
    31021 gatgaccgaa accacttctg gcaattcttc atgaatagct tcagcaattt ccttggattt
    31081 aaagaatttt tctttacgag taactaaaac gaccatcatt tcatgggaat aatgaccacg
    31141 acggataatg atgtgacgaa gctgtccttc atttttttct tcattatatc ctcgtacttg
    31201 gaaccgttgc aagatatctc tgactacttt gattgcttga tcgatgacag gatcttggat
    31261 ataaaaatct tcgataggga ctagatcatg gctattcttt ttgtaaaaac cggtggctaa
    31321 ctgtccatcg atacggcgaa cagggatctg tgctttatta cggtagccga atggttctgc
    31381 cattccgacc gttggaagta caggaacttc tggcatctta gcaatcttcg ccattacatg
    31441 ctctacttgt ttttgtttga agacaagctg ttgttcatac ttcaaatggg ctaaaggtgc
    31501 gatacctatg cgaagtaatg cattgttttc tacaggctga cgatcaggtg attgtgtaat
    31561 aaatgtttcg acttttccat atccaaactt attgcctgct tttactacta gtatttctac
    31621 ttcttctcca gggagtgcat tttcgataaa taatggataa ccatcgattt ttgctacccc
    31681 cattccttca taactcaagt cgacgatttc gactgtatat cgttcgtttt tctttactat
    31741 ttgctgttct gtcatatttt gctcctttaa gcctattttt caatcctact gaactctact
    31801 ataaatcagc taattacaca actaaaaccg gcactgacct tactacgttc agcaccggct
    31861 tttgattaat tcttttgatt aggtgtttcg tctgctattt ttccgtcacc gtcgatatct
    31921 tcatccgtta aacgttcgac ttcttttacg aattctttgc tgacaacctc taattcttct
    31981 tcatctgtac ctgtgattgc ttcatcagga atctgatcgg tattggcaaa gaactcaatg
    32041 tgttgatgaa gattttggaa atgcatcggt gcttccccgc cgtattcacc atctaagttg
    32101 atcattactg gtcgattgat atcatcttct gtttcaacga acaaactgct tgttttggta
    32161 tagattattc ttggatcatc gacatgtcga ccgccattca gcatgagtgc tgcaatatgc
    32221 aagatctcaa agatatttgc tgtcttcaca atgattaaag aaaacttccc atcatccaat
    32281 ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt tcgtcagccc taggaaaaac
    32341 atcgaagcat ttccgatata ttccccttca tcatacttca agcgcatttt gattggcttt
    32401 actcgaggga gcatctctgc tcctttcgca agataagcaa gataaccaaa aatactcttt
    32461 agttctgatg gaacttcata agtcaattct gtcaaatagc cacctgcagc gatattgatg
    32521 aagtaactgt tatcagaaag gccgatgtcc atttttacgg tttggtgttt ttttatcact
    32581 tctgctgctg ctttaatatt atctctagga atttttaatg ctcgagcata gtcgttcgtt
    32641 gtaccagctg ggatgatggc cattttagga cggtgttcaa gcggagcgat tccattaacc
    32701 acttcgttga ttgtgccatc gccaccagct gccactacta gatcaaaacc tagttctgcg
    32761 actctacatg cttcattctt ggctgaatct ggttctggtg tagttgcata ggcgctggct
    32821 tcaaatcctg cttcttctaa tgcctgtagg atatccgcca aatttttttt caataattct
    32881 tttcccgagg tggggttata aattacgcgt gcttttttca ttttcagcct ctatcgtttc
    32941 gctaattcat ctttaagtag cttattcacg acaccaggat ttgcttgtcc tttagttgct
    33001 ttcatgattt gaccgactag gaagccaacc gcacgatctt ttccattttt gaaatcatcg
    33061 acagattgtt ggttgttatc caaaatttca ttaatgattg gcagtagttt gttcggatca
    33121 gataattgca cccaaccatt tttttcaact acttcttggg cttctccgcc atttgtgatc
    33181 aattcacgga agactttttt ggcgattttt gagctgatcg ttccatcggc gatcaaacga
    33241 atcattcctg ctaagttctc aggtgtcaat tttgtctcag ctaattccag tttctcactg
    33301 ttcaagtaag cagaaacttc acccatcaac cagttagatg cttgcttggc atctgcacca
    33361 ttttccaaaa cagcttcaaa aaaatcagac atttcttttg taagtgtcaa taccattgcg
    33421 tcatactctg gaagtccaaa ctcacggata tagcgttcgc gacgagaagc tggcatctca
    33481 ggcaaactag cttttacttt ttgaatccat tcgtcatcga tcacgaattt cgggatatca
    33541 ggctctggga agtaacgata atcactagac ccttctttga cacgcatcaa aactgtttta
    33601 ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt ctcctcctga aagaagaact
    33661 tttgcttgac gtttttcttc aaaagctaaa ccttttttca cgaaagataa agagttcaag
    33721 tttttcaatt ctgctttcgt accgaactct tcttgtccat aagggcgaag agaaatattg
    33781 gcatcacagc gcatagagcc ttcttccatt ttcacatcgc tgacttctgt aaattggata
    33841 attgatcgca atgcttctag ataagcgtat gcttcttctg gtgaacgcat atcagcttca
    33901 gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat caacgtaaga ataaccgcca
    33961 ataccatgca tgttttttcc agcatcttct tctaaatgga cacgttcgat ccggattttt
    34021 ttctttttac cttctacttc gatttcgatc catccgtcat gaccgattgg ttgatcaaat
    34081 tgggaaattt gatatgcttt tggattatct gggtaaaagt aatttttccg gtcaaaatga
    34141 gtatctttag agatttcaca gtttaatgcc aaagctgctt tcatcccgaa ttccagcgca
    34201 gctttattca ttactggcag cacgcctgga taaccccagt cgatcacgtt tgtattgctg
    34261 tttggttctg caccaaaatg agctggcgct ggagagaaaa tttttgagtt ggtttttaac
    34321 tcgacgtgga cttctagtcc aatgactgtt tcaaaattca ttcgtctttc cctcctaaaa
    34381 tagctggttg ttgtttatgg aattctgttg cttgttcaaa cgcatatgcc gcttgataca
    34441 tcgttttttc atcaaaataa ttaccgatga tctgcaatcc aactggtaat ccttctgata
    34501 aacctgccgg tacagacata cctggaagtc cagccaagtt gacagggatc gttaagatgt
    34561 cactcatgta cattgtgatt ggatcgttaa tgttctcacc gattccgaat gcaactgttg
    34621 gtgaagatgg cccgatgatc aagtcataat ctgcaaagac tttttcaaaa tctcgtttga
    34681 tcaatgtacg gacttgtcct gcttttttga agtaagcgtc ataataaccc gcgcttaagg
    34741 agaatgtacc taacatgata cggcgtttta cttcttcacc gaatccttct gaacgtgagt
    34801 tgacatatac atcatctaat gatttcacat tttcagagcg ataaccgtag cggataccat
    34861 cgaatcgttg caaattggaa cttgcttctg atgaggcaat gatataataa acagccacac
    34921 cgtattttga atgaggcaag ctgacttctt ccacaacagc acctaatgaa cggaatgttt
    34981 ctgctgcttt caatacagct tcttttacgc caggggccac accttcgcct aagtattctt
    35041 taggtaaagc aatcttcatt cctttgatgt cgcctgtcag tccctctgca aaatcaggaa
    35101 ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc gctgatagct gtcaatgcta
    35161 atgcattatc tttcaccgta cgggtcattg gtccgatttg gtcaagagat gaagcaaagg
    35221 caatcaaacc aaaacgtgag acacggccat aagtaggttt catccctacg atgccgttaa
    35281 aagctgctgg ttggcggata cttcccccag tatcactacc taaagaaact ggaatttgtc
    35341 cagctgcaac tgctgctgca gaaccaccag aagagccacc aggtactttt gttgggtccc
    35401 aagcattttt tgttttcttg aaataagaag tttctgtact tccacccata gcaaattcat
    35461 ccatgtttag cttaccaaca gggatcatat ctgcttgata gattttatcc atgaccgttg
    35521 catcataaat tggttcaaaa ttgtaaagga tcttagatgc agcggttgtc aagatatctt
    35581 ttgttacgat attatccttg ataccaattg gaataccagc aaggacgttg ctttcatcaa
    35641 tccctttttc atctaaagct tttgcctgtt ccaatgcttt ttcttcgttc aatgtaatga
    35701 atgcctcgac ctcaggctct gtttctttga ttcgttcaaa tgttgttttt gtcaaatctt
    35761 gtacagtgat ttctttcgat acaagcatat gatgcagttc ttctagagaa cgattgtgta
    35821 attctgtcat tatgcgccag cctccccatt atcgatgatt gcgggtactt tgataaagcc
    35881 gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg ttccatccag cttgcgcccg
    35941 atcttgtcgc atcacgttta ctgtttcaac cacattcgac gtaaatggaa caccttccgt
    36001 atctacttct tccagcattt caaccatatc gatgatttta cctaattggt cggtaaaact
    36061 tgcaagttct tcatctgcga acgcaagttt tgctaatttg gcaacgtgtt tcacttgttc
    36121 ttcactaatc gccatactac cccttctttc taaacgagtt aaaaactttg ctctctattt
    36181 taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt tgtctgaaaa aactcatttt
    36241 tcattcttct actgagcgac ttttagccaa tgcatctgcc atttgatctt ctgtccaaac
    36301 ttcgattcct aattcttgtg ctttggtcag cttgctgcca gcatcttctc ctgctaccac
    36361 gatatccgtt tttttcgata cgctaccagt cactttcccg ccaagatttt cgatcgtctc
    36421 ttttgcttct tcacgagtaa atcgtgttag tttaccagta aggactactg ttttttcttt
    36481 aaaaggagac tcgacttctt gcaattgggc atttcgaagc cctttgtatt cgaaattgac
    36541 gcctgcttgt tttaattcat tcatcaattc atggacttct tcgttactga aataagtgac
    36601 tacactgtcg gcaatgatat caccgatcgt atcaagcgca ataatcgact catagtcact
    36661 tttactcaat gtttctagat caccgaaatg ttctgctaaa atttttgctg ctttcgctcc
    36721 gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc gaattatctt tgctgttgtc
    36781 gattgctgtc aaaatattat ttgctgattt ttcttttatt ttgtccaacg tcaataactc
    36841 ttcttctgtc aatttgtaaa gatcagcaac atctgctact aattttttat catacatctg
    36901 ttctagtacg cgcggcccca acccatcgat attcatcgca tttcttgaaa caaagtggtt
    36961 caaaccttct ttcatttgag cagggcattt aggattgatg caccgtaagg ctacttcttc
    37021 atccaaatgg acaagttcac ttccacaaac tggacaatgt gtaggcaatt ggtattcttc
    37081 gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact tctgggatga tgtctcccgc
    37141 tttataaatc agaactgtat ctttcaagcg gatatccttc aacttaatat aatcaccgtt
    37201 atgcaggctg gcacggctta ctgtcgttcc tgctactcgg actggtgtca tgatagctgt
    37261 tggcgttact acccctgttc gaccgactgt ccattcgatg ttttcgatta gcgtttcaac
    37321 ttcttctggc ggaaacttgt aagctgctgc ccatctcggt gctttgatag taaaaccaag
    37381 ttgatcttgt agcgagaatt cattcacttt gatgacgatt ccatcgattt catagggcaa
    37441 atccacacgt ttgtcatgat attcttcaat gtatgaccac acttcatcaa tcgaatgaca
    37501 tagacgtttt tcccgattcg tgtggaatcc aattttttct agctcttcta gcgcatcata
    37561 ctgggttttt gcttgcatag gaccgaaatc agctactgtg tataaaaaag tgtccagatt
    37621 tcttttcgct gtgattttag aatctaactg acgcaaagaa ccagctgcgg cgttccgagg
    37681 attcgcaaag atatcttttc cctctgcttc tctgtcttgg ttcaattgga caaatgaccg
    37741 ttttggcatg aaacattcgc ctcttacttc gatgttcatt ggttctttta atttgattgg
    37801 gaccgaccgg acagttttta ggttttcagt gatgttttct cctacagttc catccccgcg
    37861 agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa gaaacggaaa gtccatctat
    37921 ttttagttca cagcaatagt caactacacg tcccactgct ttttgtacac gttgatcaaa
    37981 agctatcagc tcttctttgc tgaaaacgtc gttcaaactg taaagcggaa tatcatgagt
    38041 gactttttca aaaccttcta atacttttcc gcctactcgt tgagtgggag aatcagacgt
    38101 gatcaaatca ggatattgtt cctcgattgc tactaattct gcgtattctt tatcataaac
    38161 atagtcttcc actgttggtt tatcttctac gtagtattcg cgagaccatt ggttcagtct
    38221 tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc gtcatttcat gttccatcat
    38281 ttaccctcca ctactaagct ttttcaattg gcgcaaaggc agccaaaaga cgttttactc
    38341 cttgacttgg gaaagccaca tctagttcta gatccttcga cgtcccgctt actcggacga
    38401 ctgttcctag accccatttt ttgtgtttga ctttatctcc aggtttccag ctgttatttt
    38461 cccctccagt agcactttta gagggaacaa ccgctgtctc tggctgttta tagctatatg
    38521 ctgttttttt agcgaataca ggcgtttttt taggcgtagg acgcatgcct tcgatttcca
    38581 gtaactcctg atcgatttct tctacaaaac gagaaggtcg gttatattgc gtccgtccat
    38641 ataatgttct ggagaaagca tttgttaaat acaacgcttc ttctgctcgc gtgatcccca
    38701 catatgcgag acggcgttct tcctccagtt cgctttcttc catcaatgct cgagataaag
    38761 gaaaaacccc ctcttctaga ccaatcagga atacaactgg aaattctaat cctttggctg
    38821 catgcaaagt catcaatgtc acttgggaag catcttcttc taaattgtca atgtcggaaa
    38881 caagtgccag atcatttaag aagaccgtta gtttttcttc tggtgcatca gcgtcttctt
    38941 cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac cgttaaaaat tcctccaagt
    39001 tttcgatgcg tgcttgtgct tctaatgtat tttggatttt taagtcttct agatatccgc
    39061 tacgatccaa aacctctttc gtcaattctg tcactgtcaa gtatgggatc atctgagtga
    39121 cttcttggat catctctcca aatgcaccta attgctgtcc tgcttttcca ccgatatttg
    39181 ccagatccac attttgcgct gcttcaagca gtggccattc atgaagagaa gcaaaactgc
    39241 gtagtttttc gatagatcct ggtccgatgc ctcgtttggg cgaattaacg atacgctcaa
    39301 aactgattga atcttgagga tttaccagca cattcaaata cgctaaaata tctttgattt
    39361 ctttacgatc gtagaacttg tgccctccaa ccattttata aggaatattt gctttcaaca
    39421 gagtttcttc cattacacga gattgtgcgt tcgtccggta caagatggca aagtcgccgt
    39481 aattccgatg attgcttcga atctcttctt gcatccggtc tacgataaaa cgggtttcgt
    39541 ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc ttctttgttt tctgtccaca
    39601 agttttttgg ttttcgattg ctattgtttt caatgacttg gtttgctgcg ctaaggatgt
    39661 ttttcgtgga gcgatagttt tgttctagca aaattactgc agcatctgga taatcttttt
    39721 caaaatccaa gatgttctgc atatctgcac cgcgccagcc gtaaatactt tgatctgcat
    39781 ctcccacgac gcacaggttc ctaaatctac ctgccaataa attgactagc gtatactgtg
    39841 cgtgatttgt atcttggtac tcatctacat gaatataatg gaatttgttt tgataatacg
    39901 tgagtgaatc aggatgttct tcaaataatc gaatcgtatt catgatcagg tcgtcaaagt
    39961 ccatacattg attatattga agctcttttt ggtaagcagc ataacatttt gccgcaatct
    40021 cttcgtaata actgccttgc atttttgcaa aatcctgagg ggtctgcagg ctgttttttg
    40081 cttgagagat tgttcctaat atacttcgag gatcatattt tttcggatca atgttcagtt
    40141 cttttagtat ccgtttcatc aaggtcagct gttcagaaga atcgatgatt gtaaagttcc
    40201 ggttataccc gataaaatca acatctcttc ttaaaatacg cacacacatc gagtgaaaag
    40261 tcgataccca gacatcttcg cctccagatg cgagaatcgc attgacacgt tctttcattt
    40321 cttttgcagc cttattggta aaagtaatcg ctaggatgtt ccaaggattg acttcttttt
    40381 cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt ctttccactt cctgcacctg
    40441 ccattagcag taatggtccg tcagtgtgaa gaacagcttc tttctgacgg gggttcatcc
    40501 cgtttaataa ttcagctttg gatgacatta acaacatcct ttcttttatc aatcttgtta
    40561 attatagcat tttcttggct ttcattaaac tagctagttc aaaatcattt gtttcttttt
    40621 atggattttt ccttgtcaat tcgttgattt cgcgctaaat taaaagaaag cgaggcgttt
    40681 gaatggaaaa aaaaggaaaa atcaagaaat tagataagac tcagatatat tttattctaa
    40741 aaggaatatt tgttggactg ctgacaggaa tcgtcgttag cctgtttcga ttatctatcg
    40801 aaaagctaag cgatttagtt cgaagtatct atgaaatgag tcgggaacag ccgatttact
    40861 taattggcct agctatttgc tgcatccttg cagcattttt cgtgggttat ctggtcaaaa
    40921 atgaaccgga tatcaaggga agcgggattc cacaagtaga gggacagctt cgcggggaat
    40981 tatcaatgaa ctggttctct gttttatgga aaaagttcat tggcggtgta ctttcagttg
    41041 gtgcgggcct ttttctaggt agagaaggac cttctataca gcttggcgca tccattggac
    41101 aaggagctgg acaattgttt cggagtccat cttcagaaga aaagatttta atttctagcg
    41161 gtgcaagtag cggacttgca gcagcattca atgctcctat cgcaggatta ctctttgttt
    41221 tagaagaaat ccatcactcc ttttctccac ttgtttggct gacatcattt gcttcagcaa
    41281 tcactgctaa cttcgtctct ctttactttt tcggcttgag acctgtttta tatctaggtg
    41341 accttccttc tttgccgttg aaatattatg gttccttagt gatcttagga gtacttctag
    41401 gcatcttagg ttttatttat cagaatgtct tgctggcttt gccaaaattt tacaaccgat
    41461 tgcctttacc agcttatttt cacgggttcg ttcctttctt gctgattttg ccaattggtt
    41521 atttatggcc tagtactcta ggcggaggca atcaaatcgt tttagctttt ggacatgctt
    41581 ccttgccaat cattatgatt atcggtttgt ttgttcttcg attcgttttt tccatgattt
    41641 cttacggtgg gggtcttcca ggggggattt tccttccgat attgacattg ggggctttaa
    41701 tcggtagctt ttatggaaac attatggttg atttcgggat ggatccaatc tacatcaagg
    41761 attttgtcgt tattgccatg gctggttatt tcacagcgat tggaaaagca ccgctgacag
    41821 caattatttt agtgacagaa atggttggct ctcttaatca tctgatgcct ttaggattgg
    41881 ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg taatccaatt tacgaatcat
    41941 tattggaacg tctacttgga aatactcaga ccgcctcact aaaaggtgca aggacaactt
    42001 ttgattttcc agtaacggca gagagttctt tagacggatt aatggtacga gacttcaatt
    42061 ggcctaaaaa tatgctgcta atctctatac gcagaggaaa tcaggaaatc ttgactcacg
    42121 gtgatacgat catgaatgta ggagatattt tggttatctt gactgatgaa gcaaacttgc
    42181 caagaatcaa gcaggaaatc gaggctaaat cttctatcac aaatttgaag caatcatttt
    42241 aacgaacatg gaaaaactat tttacaacaa ctattcatct acactacttg attctatatg
    42301 attctataaa aaaagaagag gctgggaaaa cccagcctct tcttttttat ttctatgctt
    42361 ctgctactgc aggagctttt ctcaataaac cataaatcac accagatacg actgctccga
    42421 tagcaataaa caccaaatac atgaccgggt gactcagtaa gaatacaacg aaaatcccgc
    42481 cgtgaggagc cagtaatttg attccgaaag ctccgactaa tccgccagtt aaagcagaac
    42541 caataataaa acttggaatg gcacgaatcg ggtcagctgc tgcaaatgga atcgatcctt
    42601 ccgtgacaaa cgatagtccc atcacgatgt tcgtcaaacc agcatcttga tctgttttgc
    42661 tgaatttatt cttaaacaaa cgtgtagcaa cgaaaattgc caatggaggt accattcctc
    42721 cagccataac agaagccatc acgacacttc cgccagttgc aacagtcgta gctaaagttg
    42781 ctgtaccaaa cacataagcg gctttgttga ttggaccgcc taaatctgcc gccatcattc
    42841 cagctaataa tgcaccaagt aaagcagcat tcgaaccact catacttgtc aagaaaccgt
    42901 tcaatgcatc attgattgct ttcataggga cattgaccag caacatcaag aaaccagtaa
    42961 tcagtaagcc aaacactgga taaaagagga tcgttttaat tccatccaat gattttggta
    43021 agttcttgaa taatttacgt aaaaatacga tgacataacc agctaagaat ccaccaatca
    43081 gtgcacctaa gaatcctgca ccgcctgtat tagctaatgc accagcagca aatccaacga
    43141 tcaaaccagg gcggtcaccg atacttgaag caataaatcc tgctaatacc ggcagcatga
    43201 aaccaaatgc agcttgtccg atttggttga accagctagc agcttgattg taattcccta
    43261 gatttgataa ttgatcatgt ggcacgccaa taaattggtc gatcatgaaa gaaagcgcaa
    43321 tagcgattcc tccaccaata acgaaaggta acatgtgtga tacgccgttc atcaaatctt
    43381 tgtaaattct ttgtccgata gtaccatccg catttccttc ttctgaactg ctttcttttc
    43441 catcaccatg gaatactggt gcagtaccac taagggctag attgatcaac tcttctggtt
    43501 tacggatacc atcactgaca ggacgattga tcagttcttt tccgtcaaag cgattcatct
    43561 ctactttttt gtctgctgca acgattacac cttctgcacg ggcgatgtct tcagcagtca
    43621 atcggttttt gattccttct gaaccgttcg tttcgacttt gatctccaca cccatttctt
    43681 tcgctttttt cttcaaagca tcttctgcca tgtaagtgtg agcgatccct gttggacaag
    43741 ctgtaacggc aacaacgaat tttctttctg gatgagcaga agcttctgca gcttgtgttt
    43801 cttgttctgc tttttcttct gcttcttttt gctcttcagc tgtttggaac aagtcttgga
    43861 cttcttctgg tgtatttgct tcttttaatt ttcctacgaa atctgcatca atcagcaatc
    43921 gagatagtgc tgctaatgct tgcaaatgtg tgtcgtttgc gccttctgga gctgcgatca
    43981 tgaaaaacag ataagttggc tgtccgtcta aggcttcata atctactcct ttttggcttt
    44041 ttgcgaataa aactgttgct tctttaactg cactgttttt cgcgtgaggc atggcgattc
    44101 catcaccaag acctgtagaa gtttgtgctt cgcgggctag aattccttct ttatatgttt
    44161 cgatatcgga aatccggccg gcatcaaaca ttttttggac catttcatcg attgcgcctt
    44221 ttttatcagt cgcttgcaaa tccatgatca tcgcttcttt gaccattaaa tccttgatat
    44281 tcatgattat cctccttgaa actaatcaat ttttgtgatt tcaacttctg gcagcaactc
    44341 atcaatcaat tcgcgagctg ccaagtcatc agaaaaagct gttgcgcttc cgcaagcaac
    44401 tccccattta aaggcttcga ttggatcttt cgtacggctg taagccccaa taaagccagc
    44461 aatcattgaa tcccctgcac ctactgaatt tttcaatggg cgaactaata cattcgtttt
    44521 ataagttcct tgttccgtga agagcaaagc accgtctcca gccattgaga tcagcacatt
    44581 ctgtgcccct tcagctagta attttttccc atacggaatg atttcttcta cagattggaa
    44641 ggttgtgttg taaagctcag ctaattcgtg attgtttggt ttgatcaaca acggtttctt
    44701 aacaagtgca ttttttaggt catcacctgt tgtatcaatc acgaaatctg cacctttttc
    44761 tcgaatgata tcaatcagtt cttgataaaa tccttttcgc aggctagctg gcgcgcttcc
    44821 tgacaatacg acgatatctc cttgtcctac tgttcctaat gcttgcttca actcatcgat
    44881 ttcttcatct gaaatagatg ggcctaagcc attgatctca gtttcagtgt ctgattttag
    44941 tttgatattg attcttgtat cttcttttac ggttgtgaac aaagactgga tttgttcttt
    45001 ttcaagccac ttcgaaataa attcgccagt gaaccctcct aaaaacccta gtgctgtaga
    45061 ttgagaatcg atccgtttca aaatacggga aacattgatc ccttttcctc caggtaactt
    45121 gaagtcttcc ttcattcggt tcaaatcgcc tagttttaac ccatctacgc gtacgatata
    45181 atcaattgat ggattaagtg ttactgtgta aatcattgta ttgcctcctt aattgttgtc
    45241 ttttcacgga attcattaac gaattctaat ggacaacgct cggttatgat cgtggctgct
    45301 tccaaaggtg caacagaggt gaaggacaac tggttgaatt tggtatgatc aatcaataca
    45361 aatgtctctt cggaattttt catggctaaa cgtttaacag ctgcctcttc tggatccgga
    45421 gtcgtgaatc cttgttcaag atgggcaccg ttcattccca taaatgcttt attaaaatga
    45481 aaatttttta gttgttctat cgcattagat cctaaaacag cattagttgc cagctttatc
    45541 gttccaccta aaataatggt agcaagctcc aaatcaacta aatttgccgc atgttgaacg
    45601 gaatttgtca ctactgtgat ctgttttcct tgcagaaaag gaatcatttc taacgtagta
    45661 gagccagcgt ctaaataaat gacatcttcg tcattaatgg tttcagctgc cgatttggcg
    45721 atttgctgtt tttcgtgaac gtttttgagt gatttttcac tcatattctg ctcaaaattc
    45781 aataattgct ctttcttagc tccgccatga actctttcta gaaacccact attctccagc
    45841 tcttgtaaat cacgtcttat cgttgattca gaagcattca aaaggtttac aagatcttgt
    45901 gatttcacga ttttctgctg ttcaagcaag tctaggattt tttgtctgcg ttcctctgta
    45961 agcattttca cgatcacctc aatacaaata atagcatatt ttttttctac tagcaatata
    46021 aatcagtcaa aaacgttcat aaaaattata aatcagtcaa aaaaaaaata aaaaaccagt
    46081 cagaaagact ggttttttat ttttttgtct atcatcaagc cgttttatcg ttttttattt
    46141 tcgcttcttg tttttttcat ataaacagaa gcaggttttc tttccttatg aagaagataa
    46201 taataaagtc ccatcccgcc aaacatgacc aaaaaaccga tgactgatcg aataggaaaa
    46261 ttaggaacaa aaaagagaca gacggataat aatgatgccg gaaccattat tcgataatag
    46321 agttgtttat ccattttctt cacctcatat tttttgtaaa gtaatcaatt gttctaattc
    46381 tcggtaatct aaaaattgca cacgcagatg ctgctcacct ttcttgacat cgaattggat
    46441 acttcgccgc tttttcttta ttcgtttgaa ggtaacatca gagagtctat gtttgatttc
    46501 ctttaacggt aattctgaca gggattctga aggaacatag attcttaagg ataaaagata
    46561 ctcttgatat ttttcatctg ttacctctac ttctagatat tccctaatat aataacaata
    46621 agcttcgatt ccattatcca aagcaacagt cggatcgata actttatagc acatcatgcg
    46681 tgatcgattc tcgtcaaccc aaggaaaata cgcctgagca tttagtgttc cgcgaacacg
    46741 cctttcttta taatctcctt gttggatatc ataacttttt gttacaaaaa atgaagcatt
    46801 ctttttagga acaggttgca tttgtgcatt ctgatactgg acagattgga tcgtatggtc
    46861 agtgtaaatg tattcaacga atggcggttt caataaaccg acaccattgt cccaagcaag
    46921 atgatcaaac ccaagctggt ccctaaaata ggattgaaac atttgattgt ttggcttttg
    46981 ccagttaaag gggtctcgat aaagctggat acatatgact tcgttttcta attccttcac
    47041 tttttctact tgttggatgt cgagaaaaat cggtttaggg atgatttgat gataatagcg
    47101 actataatac atttcccaac acgaagtgaa acataaacct tggtaaaaaa gatcgtatcc
    47161 tggaaattgg ttgcaatcga ctactacttc cccgttttgt tgtttcattt taggtaaatg
    47221 ttcgatttca tcctttttct caaacaatag ccgttgagag atctgttttg tattgtgata
    47281 gataaactcg ctataagctc gcatataggc gaacacccca tgagcaatca tttttttctg
    47341 aatgaagtct cgaatcaaaa attgcttttc tttgaaaaca gagatagaca atacattttt
    47401 tatcaataat tgattatctt ccaagcgaaa actacattcg ttctcgtcat ttcctacaac
    47461 taaccaactt tcgtctctta ccgatgctag cattattttt tcttgaagct ttcgaagatc
    47521 atacggatcc gcttgttgtg tgaaagagcg gataaatgtt ggctggatca acagacgtgt
    47581 caaaatagcc caatcgctta tttcctgttt tttttcttct tcatttaagt ctgtgagctg
    47641 gatagtcatc aataatttat cttcgccaat cattttctca cctctttcaa atctttttat
    47701 aatttaatta taaccaatga taaagaaaaa ctaaaacttt atgtcttaga aaccaatttg
    47761 gacgattaca tgatagacta agtaaaaaga aacaaagagg tgtttacaat ggggttgaaa
    47821 tttgaacgta cagataattt agataaactt ttcgaatcat ttgctgtcga tccggaaaaa
    47881 acagttgcac caaaagaaga gatcgatcga tttctaaaac cacagccaca aaatgaaaag
    47941 aaaaaagaca agaaacaaaa gtaagttaag ctcaaaaaaa gacttaccag ccaaactttt
    48001 atgaaagagt ttgactggta agtctttttt attcttttta tgattcaatt gcttgttcga
    48061 ttgctgccca cgcttcttct tttccctgtt ttgtaacgga agaaaagaga ataaacgtgt
    48121 cattgcgatc aaaatccaac gcttttttga tttcgctctc atgcttgttc catttaccac
    48181 gagggatctt atctgcctta gtggcaacaa caattacagg gatatcataa tacttcaaaa
    48241 attgatacat ctggatatct tcttgagaag gcttatgacg caaatctact aaagaaacga
    48301 ccgcacgtag ctggtctcta gtcgttaaat atgtctcgat catctttccc cattttgctc
    48361 gctcagtttt ggatactttt gcatacccat atcccggaac atcgacaaaa tgtaatgcgt
    48421 tctcgatcaa ataaaaattc aatgtctggg tctttcctgg cttaccggaa gtacgggcta
    48481 aattctttcg atcaatcaat gtgttgataa aagaagattt ccctacattt gatcgtcctg
    48541 ccagagctat ttctggcaat tgtgtatctg gatattgttt tggtgccact gcacttatga
    48601 taatttcagc ttgatggact ttcatggtct ctctccttaa caaggtgatg aataaagcaa
    48661 gcggctgtcg caaaagccaa aatgactttt acggcagcct cttttcatca acccgctttt
    48721 ttctctgaat ctttatagat tactgttggc tcaccagaca attcagctgc ttcttttgtg
    48781 ataatcactt tttcgatgtt ttcatttgaa gggatatcaa acatcacatc catcatgatt
    48841 tcttcaatga tggaacgaag tcctcgagca ccggtattgc gttcgattgc ttttttcgca
    48901 atggctctca acgcttcagg ttcgaattca agttcggtat tatctagaga aagaagtttt
    48961 tgatattgtt tgactagcgc atttttcggc tcagtcaaga tccgtaccag atcatctgtt
    49021 gtcaatttct ctaaagctgc cataacaggt aatcgtccga taaactcagg gatcaatccg
    49081 aattttagta aatcttcagg aatgatatgc tgcatgacac tttcgtcttc agaaagtttt
    49141 tggttttgtg taccaaatcc aatcgtcttt tcgcccatac gattcttcac aatcgtttcg
    49201 atgccatcaa atgcaccacc tacgatgaac aatatgttcg tcgtatcaat ctgaataaat
    49261 tcttgatgtg gatgtttacg tccgccctga ggtggcacac ttgcaacagt tccttctaga
    49321 attttcagca aggcttgttg gacaccttcg ccggaaacgt cacgagtaat agatacattt
    49381 tcgcttttac gcgcaatttt atcgatctca tcaatataga tgatcccttt ttctgcaagt
    49441 tccacattgt aatcagcaga ttgaagcaat ttaagcaaaa tgttttctac atcttccccc
    49501 acataaccag cttctgtcaa acttgtcgca tctgcgattg caaaaggtac attcaatgtt
    49561 tttgctaatg tttgtgctaa aaatgtttta ccagaccctg taggtccgat caagcagata
    49621 ttgctttttt gcagttcaac atcatctgtg ttttcttgtg cttgctgatt gacacgttta
    49681 tagtgattgt atacggcaac agctaacgag cgttttgcgc gatcttgtcc aatcacgtag
    49741 ttattcaata cttcaagaat ttctagtggt ttagggactt cagtgaattc gcgtacagct
    49801 tcttcataaa attcttcatc aatgatttct ttacataggt cgatacattc attacagatg
    49861 tagacccctg gaccagctac gatctttttc acttcttctt gggttttccc gcaaaaagaa
    49921 cagcgtacgg tctcattgct acttggattg tcatacatgg tcttcacccc ttaacttgca
    49981 aaaagtatgc aagaaactat caaagacttt acagtctttt actatcataa catagtttag
    50041 taaaaagaaa aagtgtttaa caaaggatca actgacctat acctttgcat tttttactct
    50101 taacaaaaaa taaggaactg tgacaaaatc gtgtcacagt cccttatttc gaataaacaa
    50161 ttttctagaa gtagcttctt cagaaatgat ttattacttg atttctattc tttcgcttct
    50221 gtcgctgtac ctgtgatcaa ttcgattgct tttttcattg tcacatcatg ttccagcata
    50281 tcttctgtca acacgcgttt gatttgttcg attggcatgt tgtatgtttc tgataattct
    50341 tggatttctg cattgatttc ttcttctgtt gcttgaagat tttcagcagc tgcaattgct
    50401 tcgatcacaa gatttgttct tgtgcgcatt tctgcttcac cttcgaattg tttatgcaag
    50461 tcttcttctg tagaaccagt taattgatag tacatttcag gagagatacc ttgacgttgc
    50521 atgttgttca agaattcgtc cattgaacga tgtacttcat catgaaccat cacgtgtggc
    50581 aattcaacga tttctgcgtt ttcaacagcc atacggatcg ctgcttcatc ttttgcatcg
    50641 tccgctgctt tttcttttgt ttctactaat tctttgcgat atttttcttt caattcgtct
    50701 aatgttgata cttcgtcatc aacatctttt gcgaattcgt catctaattc aggcacttct
    50761 tttgctttta cttcatggat tgtcactttg aagactgctt cttttccagc aagatcttct
    50821 gcttgataat cttctgggaa agttacattt acatctagac tttcacctga tttatgacca
    50881 actaattgat cttcaaaacc tggaatgaat gagcctgaac ctaattcaag tgagtaattt
    50941 tcgccttttc cgccatcaaa cgcttcgcca tctacaaagc cttcgaagtc gattactact
    51001 gtatcgccat tttcagctgc agcatcttct ttgataacta attcagcttg tgcttcttgt
    51061 tcacgtttga tacgtgcatc cacgtcagca tctgttactt cacggtcttg tttttcaaca
    51121 actagatctt tatattgtcc taattttact tctggtttca ctgttacttc agcagtgatt
    51181 acccagtctt gacctttttc catgctttca acatcgattt ttggttgaga aacaggatcg
    51241 atgccagctt ctttgacagc agcttcataa gcttctggta atacagcatt caatgcatct
    51301 tcatataaag cttcttcacc gtacatacgg ttgaatactt gacgagaaac tttcccttta
    51361 cggaatccag gtacgttcaa gctttttttc actttattaa acgcagttgt taatcctttt
    51421 tggatttcgt cttgcgcaat cgtaaatttt aaaacaccat cattcgtgcc ttttttttca
    51481 aatgttgcag tcatttaatt ccctccgagt ttagaaattc attttataca tcaaaaacgt
    51541 ttcatgcata cctttaaata gtacactaac cctttcaaat tgtaaacata ttgaatggat
    51601 agaatccttg tttttcataa catttttatc tttttcactg ataaaatcta atggaagaag
    51661 atcatttgct gcaaaatttt ttctcttttt tttaaaaaat cttgtttttc atcttctgta
    51721 ccttcataca tcgtttttcc tgtacacgcc tctacagtca gttgaaacca gcgcttcggg
    51781 tcttcgatgt atgtatcata gacaggataa acaattgcag attgcaacgt gaattcttct
    51841 tttaacacgg aactaagcag cgcatcttca ttcccgtaac gatctgaaaa tacacataat
    51901 tgctggtaac ttgcctgctg ctgcggcttt ggcagatctt ttggtatgac tttgactaac
    51961 ttttcttcaa tcgtcaaata gaaaacagga tggcattcgt tcaattgcct aagtgtctcc
    52021 agtaattttg cccttgctaa aatatggacc tctggcgata caataagttt actcgctgtt
    52081 tggataaact catgataagg aagctgttcg atctcatcaa tagacgcaag ctgacgtgca
    52141 ggttccaacg aaggaagttc ctccgattta ttgtggagtg ccttgatctt ctgcctctcc
    52201 atttgtccgg ataattcttc tgcaaattgg attttttcaa tgacccgctg ctcgaaagct
    52261 tcataaaaat ctgtacttgc tatcaatttt ctagcatata aaaaatcttg tgtactgatt
    52321 aacaagtcaa aataaaattc agcaaactcc tcattttgaa gatactgttt ttcatgcaag
    52381 agtgcttgct tcaatgcttc ttttttctcg tccaactcgt aaagacaaaa aacaagcagg
    52441 ctattagcct caaagctttc tttcaactcg taagctcgtt gaaataattc ttttgcctgc
    52501 tgatattgct ggtttgctag tgcttgttgt gccactcgca ttaaattctc atatttttct
    52561 ggaaaatcaa tcggcttcac gcagtgtcct cctcttgctc atattcagct catgttcaat
    52621 agtagcaaaa caaaaaaagc atgacaatta aaaataactg aagcaccctc taaaagctag
    52681 attttagatc tgacttttag aaggtgcttt caactgttac tgctttttat tgctcatcag
    52741 ctgttgactt cctggttgaa cgctttgatc agattgatcg taacaacact tgtattagtg
    52801 aaaaaatcgc ccagatgttg ctttttctga ttaaagactg ctggaagata ccctaacatc
    52861 agcaaaggaa attttagaat gaaccggcaa gcaccttctc taactaaaac ggttgaccag
    52921 ctaagttctt cttcatcaaa gctgatgact cgaataccaa agatcatttt cccaatcgtt
    52981 tggccatgat tgaatttcgt caataaaata aaatacgcta gataaataag taaggctgtg
    53041 aatccgtaga gactcagata agaatctgaa aaattaagcc aacctagatt tttggcaata
    53101 ccaagtgtta aacgcgtaat agctccgata cacaaaagat cgaccaagaa ggcaaacatt
    53161 cggataaaaa aacctgcata gaaatactta ggaaaatcat ttgcttttgg cggttcattt
    53221 cttgtgtccg tatacttttt ccatttacgt tgtttctttt tgatttcttc gggcgtcaaa
    53281 ggctctcttt caaaagagct cagtaccttt tgggaaaagt tggagtcagg tcctacagca
    53341 ggctgctgtt gcttatcttt ttcttgttcc ttattttcat ttgaaagctc tgacatgctt
    53401 actcacctcc gtaataatac attgcttttg gtgattccgc tgtccctaga ttctcaagaa
    53461 cggataagat ctgactagtc tcagaaggct tcaatccctg gaattctgct agtttacttc
    53521 ctaaccatgt actagcaaac ccagatggcg tggaagaata ttctactagt tccgcatctt
    53581 caagctgttg ttcctttcgt atagctttta aagcatcttc aggaaatccc aattcatcga
    53641 ctaaacctac ttcttttgct tggacaccat cataaatcct tccatcagca atttttttga
    53701 cttcttcttc agatttattc cgtccctcgc tgacgattgc tacaaaacga ctatatgcgc
    53761 tgtcgatata tgcttgaagc accgcttctt cttcttttgt ctctggtcgc gttcctgaac
    53821 ccatatcttt caacgctccg cttttaactg ttgtatcttc cacacccagt ttttctagaa
    53881 gtcctgaata attcaatcct gacatgatca caccaattga gccggtcact gtttcttctg
    53941 ttgcaaagat tttatctgct tgagcagaaa tatagtagcc accgctagct gccatgtttt
    54001 tcatgctgac atacattggg atatcatgtt ctttacgaat cgtatccaat ttcttagcga
    54061 tttccgcact ttcgtagaca ccgccaccag gagaatttac ttctaaaaag atacctttga
    54121 ctgttggatc ttcttcaatc gccttcagtt gttccataaa atttttatga ttgtaacctt
    54181 cagaagcaaa taaacctgaa gaccctcccg cagcaatcgt tccatctact gataatttta
    54241 cgattttttg cgtactgtct ccctcttcca acacatttgg agctagttct tcatccccat
    54301 ataaccaact gttcaatccc tcaatttgat cgttttcctg aggcttagtc aaactagctg
    54361 aaactaacga aaccacaaat aatcctgcag caatcagaac tgctgcccaa cgtcttttat
    54421 tcattctttc tcctttctaa tacaagtttt ttatgacgat cacagtttct gactgctcag
    54481 aagtgtcgaa ccgctctgct ttttcttccc gcgcataccc ggaaaacgac tcttttgtta
    54541 attcatcttc taccgtaaac tcacgagctt gagcaacttc caaggcatca ggtgcgattt
    54601 cttggatgct atcccagcca gtatctaaaa atattttcgt atcatctgat acaccagctg
    54661 atgcttcgaa tttggacaat tgttttttta acattcccag tgtcatgatc tcaatttttg
    54721 aatccatttt tactcctcct ctatccgatt actttctatt gtaccgaact cctgaaaaag
    54781 atgatagata aaaacccatt attttttgac ataaaaagga catttgacaa agaatttgat
    54841 atataccaat ttatttttta ttcttttttt gttgacaaga acattcgcaa aaggttatga
    54901 ttttaaatgt ttccagataa aaaacaaaaa ggaggatagt tatacctttt aggatagcgc
    54961 caggtctgtg taaacagatg acgaggagag ggtttatcga aagattcggc ggatggccct
    55021 agggactgca ctctacagaa aatccacaaa aaatgtctgc aaagataaaa caaaaggatt
    55081 ttcctgcagc tggaagcatt catacgaatt cattatagaa agtaggatgt tttttatgtg
    55141 cggaattgtt ggaatgattg gattaaaaaa tgtaacacca ggattgattg acggtctaga
    55201 aaaattagaa taccgtggtt atgattcagc tggtatcttt gtctcagatg gcacaactga
    55261 ttatctggtc aaagcacaag gcagaattca aaatttgaaa aacaaaataa atattgatac
    55321 tacaggaaat ataggtattg gtcatacacg atgggcaact cacgggcaac cttctgaaga
    55381 aaacgcccat ccacatactt cccaaagtgg acgcttcgtg ctcgttcaca acggcgtaat
    55441 cgaaaacttt gaagaactta aaattgctta tttagcaaat gaccacttta ttggtgagac
    55501 cgatactgag attatcgctc atctaattga aactttcgcg aaggatacta caacgcaaga
    55561 ggcgttttta aaagctttgc gggtcatcaa aggttcttac gctttcgctt tgattgatcg
    55621 gacagcacct gatgttattt acgtcgctaa aaataaaagt ccactattaa tcggactcgg
    55681 cgatggattc aatgtgattg ccagcgatgc aatggctatg cttgctcata cgaaggaatt
    55741 cgttgagatt gaagatgaag agatggttac cgtcacatca gaaaaagtta ttattcaaaa
    55801 ttttgcaggt gacatcgtag aacgttcttc attcgaagca caagtagatg cttcagatat
    55861 cgagaaaggt acctatcctt tttatatgct gaaagaaatc gacgaacagc ctattatcat
    55921 gcgtcgtatc gctcaaaaat acgtaacaga agacaatcgt gtctctcttg atcaaaagct
    55981 ggtcgataca ttgtctgata gcgatcgaat ctatatcgtt gcttgcggaa ctagttacca
    56041 tgcggggtta gcaggtaaac agactttgga aaaattgaca cagattcctg ttgaggttca
    56101 tttagccagt gaatttggct ataacacacc tcttctttca aaaaaaccat tctttatttt
    56161 cttgagtcaa agtggcgaaa cagcagacag ccgccaagtg ctggtaaaaa tcaaccgtct
    56221 cggctaccct tcacttacga ttacaaatgt tgctggttct actctttcgc gggaagcatc
    56281 ctttacactg ctattgcatg ctggacccga aatcgcagta gcatcaacga aagcttatac
    56341 tgcacaaata gctgtactaa ctttattagc caaagctatc ggtgataaaa aagaatttga
    56401 aacttctctt gtattcgata tcactcatga attgagttta gttgctaatg cgatggaatc
    56461 ggtcatcgcc caaaaagatt atttagaaga gttggcagaa gtatatctca gcgatacaag
    56521 aaatgccttt tacattggac gtggtgctga ctacaacgtt tctcttgaag ctgctttgaa
    56581 attgaaagaa atcagttata tccaggcaga aggttttgcc gctggcgaat tgaaacatgg
    56641 aacgattgct ttgatcgaag aaggaacacc agtgatcgga atcatcagtg aagaagtgac
    56701 tggtgcgcat actcgaggaa acctgaaaga agtcgaaagc cgtggcgcta aaacactagt
    56761 tattgtctca gagggattag aaaaggaaag tgaccagctg gtgctaccaa ctgtccaccc
    56821 ttatctaact actctaacca cagtcgtacc gactcaatta cttgcatatt acgcaacgct
    56881 attacgggga ttcgacgttg acaaaccacg taatttagcg aaatcagtga cagtagaata
    56941 ataaaagaac aaggaccagt gtcagacatc tgacactggt ccttgttctt ttatttttta
    57001 tgaaaatatc tcttaatcca gtccgatttc ttctttgact actgccgcga ttttatcgac
    57061 atagtaatta actttctcat cagtaggcgc ttctgccatc actcgaagta atggttctgt
    57121 accggaagga cgaactagga ttcttccttc tgatcccatt tcagcttctg cctcttcaat
    57181 cacttttttg attgccggaa catccatcgc accgttttta tcacttacgc ggatattcac
    57241 taatttttgc ggatagattg ttacctctgc tgcaagctct gataatttct tacctgtctg
    57301 tttcatgata ttcagtaact gaattcctga aagcatccca tctccagttg tgttaaaatc
    57361 taagaaaatc atgtgcccag actgctcgcc gccaaagtta taatcgtttt tgcgcatttc
    57421 ttctacaacg taacgatcac caacttgtgt aacgacatct ttcaaaccga ttccttccac
    57481 cgctttgtgg aatcctaagt tactcatgac agtcgtaacg atcgtatctt ttttcaatcg
    57541 atttttttct gccaagtatt tagcacagat aaacatgatt ttatcaccgt cgacaatgtt
    57601 tccaagttca tccaccgcaa taatacgatc gccatcgcca tcgaaagcta gtccagcatc
    57661 tgctcctttt tcaacgacca tctcagctaa acgttctgga tgggtagagc ctacgccgtc
    57721 attgatattt aacccgtctg gatttgtgcc cattgtataa aaatctgttt caagatcggc
    57781 aaacaaacga ttaacacttg ttgaagttgc tccgtttgct gcatcgacac aaatggtcaa
    57841 accggaaagg tcaccgttga tcgtttgtac aaggaattga gaatatttca ataagccttc
    57901 tggaaattct tctacagttc ccaatccttc agctgatgga cgaggcaatt catccacttc
    57961 agcatctaat aatgcttcaa tttctagctc ttggtcatct actagtttaa aaccatctgc
    58021 accgaaaaat ttgattccat tatcttgtgc tggattatga gaagcagaaa tcatgacccc
    58081 tgcgcttgct ttttgcaaac gtgtcagata agcaacacct ggtgtagaga tcacacctag
    58141 ctggaacact tcgataccca ccgataaaag tcctgaaatc agcgcttctt ctaacatttg
    58201 acctgagatt cttgtatccc gccctactaa cacgcgtggg cgttttcctt cttcttcatg
    58261 ctggcttagg acataacctc cgcaccgtcc cagcttaaat gcaagttctg gcgtaagttc
    58321 cttgttggct tcaccgcgta cgccgtctgt tccaaaatat ttacccatag ttgtaaaatt
    58381 actcctttaa cttaattttc tgaggaattt gttctgctca tttgtgttga ttcgcttgtt
    58441 tctgatgttt cttctgtact tgtggtcgag ctgcttgtcc cagattccga actggaagaa
    58501 atggttgttg tcgatggttc cgttcctgaa tcagcagatg aaatacttct actcgtactt
    58561 gtattttctt gtgtccgact gctcgtcgaa gcagagctac tgcttgacgt ttgcaacacg
    58621 ggtgtaatct ccacactaac tgattcgggc ttgatactta acccctcttc tactggtatt
    58681 tcaattttac gagttgttcg atgattgata cctgagacat tgatcggtac agcaatactg
    58741 tcaccgatcg atgcaagcaa atcgctagag ccgcttactt ctgcttccag ttttgaaaaa
    58801 ctgaaatcat agctttctac cccggcagct ggtgttcctt gctgtatccc ataaagtcgt
    58861 accgacttgc ttggcttgat cgaatcagct ctcactgtta cttgcactgg atcagagacg
    58921 atacttaata cttcccctgc tgcattcagc gcctgtattg ggacctcact attaatccca
    58981 tcatctgtga ccatcgaagc gtccacatca gctactactc gtttgatttc tgccaatgtt
    59041 ttgtcacctg tagtgataga gactttttta ggactaacgg aagtatcatc aagttcatat
    59101 ccagatggtg tgaacgttga aggaatcaac gtttccacat cgaaatcttt tattaccttt
    59161 ttctcaaccg ttactgtaat agtcgatggt tcgatctttg ctgtgacagc actgctcaaa
    59221 ttttgaattt ttagcggaac ttcatgtgtt ccttcgccca gcttagtgat atctgctgtg
    59281 acgcggaaca tccgtgtatc ttcgtttgcc tctgcattca actgaactcg gtttgcactg
    59341 gaaagtttga cagtcactgc attttcatat ccatggatat agtagcgctt tgaatcataa
    59401 gaaatcttga tcggcacccc cgtaactgtt tgattatacg cttcatttcc agaaagcgta
    59461 tttggaacgg actggccatt tgcattgaag tacagcgcaa tactgaacag caatgcaata
    59521 aaactgtaaa gaatgcttgt ccgtttctct tttgagatca tttgcgatcc cctcccttag
    59581 tcataccgtc taggaaagat tgaatcacgt ttttcctgtt ttttgttttc tcttcagcaa
    59641 ttagttctct acgcaagatt gccaaatatt cttgctggga taagccagcc atcagttcat
    59701 tatctaacgt gatgctgacg tccccggttt cttcagagac aacgatggta atcgcgtcac
    59761 tgacttcgct tataccgaca gctgcccggt gtcgagtccc gaattcttta gggatcaaca
    59821 tactttctga tagaggaaga taggcgcttg ctactgcgat ctttccttct tttacaataa
    59881 ctgcaccgtc atgcagcggt gtattaggaa taaagatatt gatcagcagc tccccagtga
    59941 tatctgcatc taaagcaatc cctgtctcaa tatactcatc taatccagta tgacgttcga
    60001 tagtgatcaa cgcaccgatt tttcgcttgg acatatattg gatggctttg tcaaaagaca
    60061 agatcatctt ctcatcttcc tgctgttcgc tttttgattg tttgaaaagc gaactgcgtc
    60121 caagatgttc cagtcctctc cggatttccg gctgaaaaat cacgatagca gctatcaccc
    60181 cgtacgtaat gacttggttc atcagccaag acaaggtgtg cagaccaatg atttccgcta
    60241 cgattcggat aacaataaaa atagctacac ctttcagaag ctgaatcgct tttgtgcctc
    60301 gcagcagttg gatcagtttg tatacaagat accagacaac cagaatatca agtatgttca
    60361 ccaaaaaatt cagagagaac aagtcaccgg aaaacatttg ctgccaatag tccaaattaa
    60421 aaagttcccc gatttgaaat gacataaaaa gcctctcttc tcttttgaga tttcctctct
    60481 agtatatcat atgttttaat tttagaggct gatatgctcg tatttttctt gagaaaaacg
    60541 cataattttc aagaaaattg caagtatgcg ttttattttt ctctgcaata aatattttta
    60601 aacttcagag aattattcat tatatttttt ctgttatttt tccttttttt cttttgtagc
    60661 ttgttccatt tttttacgtc gttctgctct ggcagctctt ctagcagctc ttctttcttc
    60721 tgcttcaagg tccgtcgttt tttcttttct agtcttaacg ggtacatcga tagattcctc
    60781 tggttcaagt cgtttcttga ttttttctaa ttctcccgtc agtgcagcat agttgtaaaa
    60841 acgtgtttta gcgtcgtgga cagtcttttc aaataaagct tttgcaagat ttggatttgc
    60901 gttatctagc gaggaaaagc gaacttgttt cctcatgaaa tcaggcatag cagagaaatc
    60961 aggttttttg tagtccaaag tcattgggtt cttgtttttt tcgaccattg tagggttata
    61021 tcgatataat gaccaataac cagaagaaac agcttctttt gcttctttaa gcgattcttt
    61081 cattccgcca agcaagccat gtgtgataca aggcgtatag gcaataatca gagaaggacc
    61141 aggatacttt tctgcttctt caaatgcctt gatcgtttgc atttgattgg cacctgaagc
    61201 aatttgcgcc acataaacat ttccatatgt catcgccatc attcctagat ctttctttga
    61261 agcatacttt ccactagccg aaaatttagc gattgccgaa gctggtgttg cttttgaggt
    61321 ttgaccgcca gtattggaat agacttcgtt atccaaaaca aggatattca cgtctgctcc
    61381 gctggcaagt acatgatcga tcccgccaaa accaatgtcg taagcccatc cgtctccacc
    61441 aatcatccat tggctgtttt ttacgaatag atcctttctc ttgtatatat cctccagttc
    61501 agggaaaatt gatcgttcgc tttcgatcgc agcttgcagt tttgctgctc tttgctgcgt
    61561 tccttcactc tcaaacatgt gtactaacca gtcttccatc aattctcgta gttcttctga
    61621 tacttttggc aatacttctt tgattgaact tgccaaagcc tcgcgacgag tctgtgccgc
    61681 tagaagcata ccataaccaa attcagcatt atcttccaat aaagaatttg accatgcagg
    61741 cccttgacca cattcattgg tcgtataagg agaagcagga gaagcaccgc cccaaataga
    61801 agaacagcct gtcgcattcg cgatcatcat tcgatcaccg aacatttgtg tcaataattt
    61861 tacataaggt gtttcgccac agcctgagca agcgcccgag aattcaagaa gcggttgatt
    61921 gaattgtgta gacaagacag tattctttcc tttgactgga tttgcttttt gtcttaatgt
    61981 cattgcaaaa gcccaattca cagcttgttc tttctgttct tcataaggct tcatcactaa
    62041 agctttttct tttgcgggac aagcctctac acacagacca caacctgtac agtcttccac
    62101 tgaaacttgg atacggtatt ttacgccgtc tgcgcctcgc atttctctca cgataaatcc
    62161 ttctggcgct tcttccattt cctcttcatc tgctaaaaat ggacggatgg cagcatgagg
    62221 acaaacaaag gcacattcat tacacatcgt acagcgatca ctaatccact caggtacttc
    62281 taaagcgacg ccccgctttt ccatagaagc cgttcctaaa ggcatttcgc cagttgtcat
    62341 tccatttgct acaagtgtac caacagagag ttgatttcct tcctgtgcat tcactggttc
    62401 aagtatttct tgtatataac cagtgttcct tttattgatt ttttcaggca tctccaacgt
    62461 tctccaatgg tctgggatcg cgacttgatg aagcagttcg gtcgtccgat cgattgcttg
    62521 tatgtttttt tctacgatag ccattgattt atgtccatag cttttcaggg cttcttcttt
    62581 tagcagcgga agcacctcat caaaaggaat gatgtcagct aatttaaaaa aagccgtttc
    62641 cattgccgta ttgatccggc gtcccaaacc gacttcttgg gccaatctca tggcattgat
    62701 cgtatagaat ctaatgttat tttcagccaa gtaacgtttt aattttaagg gcagatgcgt
    62761 ttctaattgt tcgtcgctcc aaagagtatt caataaaaaa atgccacctg gcttcaatcc
    62821 tttgaccaag tcatagctat gcaggtaagc tggtgtgtga caggcaacaa ggtccgcatg
    62881 ctcgacaaga tacgctgaac gtatcggcgt atctccaaaa cgaagatgag agacagtcag
    62941 accgccagat tttttcgaat cataataaaa atagccttgc gcatatttat cggtgtgatc
    63001 tccaataatc ttgatagctg atttatttgc tccaactgtt ccgtcagatc caaatcccca
    63061 aaacttggcc tggaatgttt gcggttctgt caaatccaga ctttcctttg gttccaaaga
    63121 ttgataagtc acatcatcca cgatccctat ggtaaaccgt tttttgcgta tgcttgggtc
    63181 ttttttcaat tcatcaaaaa ctgctgagat ttggtcggga gtcacatctt tagaccccaa
    63241 tccgtagcga ccaccaataa cagccgggcg aagttcagaa tcatataacg cactttgaac
    63301 gtctaataag agaggttctc cacctgcccc tggttcttta cttcgatcaa gaacagcaat
    63361 tgtttttact gtatgaggca acttttgcac gaatgtttcc actggaaaag gacgataaag
    63421 atggatattc aaaaaacctg ttttccgtcc gtttgcattc aagtaatcaa tcgtttgttc
    63481 aatcgtctgt gcaactgatc ccatagaaac aatgacttct tccgcatcgt ctgctccgta
    63541 ataagtcacc aagtcataag aagttcctcg taatcggttg atctctttca tatatttttg
    63601 aacgatttca ggtaatttat cataataggg gttgatcgtt tcccgttgtt gaaaatggat
    63661 gtctggattc tggttcgtac cactaacagt cggatgattg ggattcatcc ctcgtttacg
    63721 aaatgaagct aatttctcct gattgatcag aggagctaat tcttcatatt ccaaaacttc
    63781 gattttttga atctcgtgac tcgtacggaa tccatcaaag aaattcaaaa aagggacttc
    63841 tccttcaata gaagcaagat gggcaacagg tgctaaatcc atcacttctt gtacactgct
    63901 ttcagctaac atagcaaatc ctgtttgtct tgcagccatc acgtctccgt gatctccgaa
    63961 aatattcaac gcatttgtcg tcactgcccg actagcaacg tggaaaacac ttggcagtag
    64021 ttccccagca atcttgtaca tattcggtat catcaaaagc agcccttgtg aagcggtata
    64081 tgtagttgtc agtgcaccag ttttcaatga tccatggacc acaccagctg ctcctgcttc
    64141 ggattgcatt tctactactt ttactggttg accgaatacg ttttcttttc ctgctgctga
    64201 ccattgatct actaattcag ccatggtcga actgggtgtt attggatata ttgcagcaat
    64261 ctcggtaaat gcataagaaa tatatgcggc tgccgtattg ccatccattg ttttcatttt
    64321 tcgcatgatt catcatcctt gttcatattc tctcttatta tctgttccat tataacgtaa
    64381 aacatgccat ttttttcttt tatttataaa aatattttac atgtatctat ttgaattggt
    64441 catctaattt ttagacaaac agtctaaatc gttgcctttt tttgtcagac atgtataaac
    64501 tataagcgaa agaggtgttt taacatggat atatcagtga ttgatgcaac gaaaacaaat
    64561 acacaaaaag gaatccttta tggttcatcg aatgctccta aaaaaatggt cgaattcatc
    64621 aacttagctt gtccgtattg ccgacaatgg tttgaagaat cttatgagct attagaagaa
    64681 gcagtccaaa gcgggcaatt gcagcgagtg atcaaattgt tcgataaaga aaaagaaagc
    64741 ttacagcgag ggaacgtgat gcaccggtat ctcacaatca gcgatggcca aaaagcgatc
    64801 aaagagatca aacaaatatt cgatactcaa gacgagtgga aacatctttc tctccaagaa
    64861 gtagcggatt ttgcagtaga taaactaaag ctgacagaac aaaaagacga acagctgagc
    64921 caagctgtca tcaacgaagc tgagcaagca catattcgat ttgttccaac tgtcatcttg
    64981 ggtaaagaaa tctttgatga atctatttca atagaggaat taaaagaatt gatccaaaca
    65041 aaataaaaaa ataaggagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    65101 cacgtataaa cggcggaagc agaagcaact ccttcttatt tcttggagtt aaacgcttct
    65161 gtcccagcct ccttattccg aataaatggt gtccaaaagc ttgcatctgc cagattatct
    65221 catttcttat gacaagctaa gcaaattatc tccaccaaat aatttattca tatttttgac
    65281 ttgcgcatac taactaattc ttaaattttc atacaaaatt tctcaatttc ccttaacgag
    65341 taatatttat gtacggtgat tctcactagt atcccaatca ctttttaagt tatcccgatt
    65401 tcaattctac ttcaaaaata gttgctttta tgtatgctgt tagttgatta gatggtcatt
    65461 tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt agcttatacc tatctcgtct
    65521 accaaacgca agatggcaaa cacaaatgca tacgcatctg tatttgccac tttgattcgt
    65581 ataaagctaa caactattaa ctcctctcag ctaatagttg ctctcttttt tattcagatt
    65641 catttttgct gatcacagcc tagtatttca atggttttga acactaaacc aaattgttcc
    65701 attacaacat tcactggctg atgaaatgga ttagtagatt tcgattattc aaagcgattt
    65761 tctattggtg gatcgtgttc ccaactaata agacggattc caaacctatc gtacagatct
    65821 tctaaaccac ttataaattt tgcacctatt tcaggtgttt tattgcgtgg atattctatt
    65881 gttacgccat taaatggata ccaattagga ttaggctctc tttttttctg tacatctccc
    65941 atagaatagt tagcactttc tgggacataa tatattcttc gaccatcctc taattcatct
    66001 aataagatat ttcgtgatcg tctatcactt gctaaatgta gacgccacgt gcgataccat
    66061 gccatgctac ctgcatctgc agaaaatcca tcaaataatg caacaattgt atcttctatc
    66121 acaatccttt ccccatctac ttcatattct aatctaaaat caaactcccc tcgggtaatt
    66181 tctggtcttg gtggcttagg acccaatatt attctactta aaaccgcatt cgttcccaga
    66241 tgaaacacag ctataacaac aataatgatt aatattttgt ttctatttga taaatttctc
    66301 attattcgca catcacctct acattctcac atattagttc gtaatactcc tttaatatca
    66361 aaatatctac tggtcgtaat aatattcgct atgaattcct actactcgta taagaaaggc
    66421 caaatcatca gcttttactt gctgatgatt tggcttgttt ctatttatcg taattgtttt
    66481 ctaatcacca aaattgttaa caatcggttc agatatttcc cagttgatta aggtaattcc
    66541 gtattgtgtc aaaacttctt gcgcttctcc gaaattaagt acagttctta tgtatccact
    66601 ttctcctaat ataaagtcta cctctctttt aaaaccttcc tcgagatttc taagggctat
    66661 atgtggtttt aatgacattg attgtacatt ttcccccata taatattgcg cacttcctcg
    66721 actaaaatag attcctagat cgtccccaaa aggtagatct tctctaccac tagctaatct
    66781 acttctccat cttcgttctc ttttcataga acctgcgctg aactcacacc cgttaaactc
    66841 agctatgaca gtatcttcaa ttatgttaat ctgtccatct agttcgtatt caatcctaaa
    66901 tggaaattct ccataggtga tttccggttc gggtatttta ggtgagatta atgcaaagaa
    66961 aggaaatatt aagcttggca acatcaaaaa aactcctcct atgatcacaa ttcccgatgt
    67021 aatccaaaat ataatttgta caattctcat tgatccattg tattccattt tattccccct
    67081 attactattc atatccgcct atctatatcg atcttccaat gcagagataa ttgcttccat
    67141 accttgattt tttactgagt tacctatgcg agatatcatt ctccactaag gtgcatcata
    67201 ttgtctatct aacatgatta attctccaat aggtgtatac tcctccaaaa acaacattta
    67261 atgcatctcc ctcgacaaca taagctacca tgtttgctgt atatgtgttt gagtctaatt
    67321 cataagcaga taaatttact gtcgctggat taaatataat cgcattcgta tttatagcta
    67381 ctgaatttgc tgcttcagca ccaccttttg aatgtccgat cattgtgatt tcaatagtag
    67441 gatttacgtg gacagccctc accgtttacc ctgaccatta tagaaatcgc ttactttttt
    67501 gaactacgca acaaaggata caatgctcct aacttcactg tctccatttt tgtccactaa
    67561 ggaatataaa aaataggcat taattcattt aattcggtca caacacgtta ccttttaaat
    67621 cattatactg tatctcatac attattatca ataaactatt ataaaaatgc aaaaaatgct
    67681 ttatcaatct attttgtaat tggtcataat atcattgaaa tcataccctg tatcgatttt
    67741 tcctatgaat ctcgtctatt tgtgattgac gaaaaagagg caagacagac actggaatgg
    67801 taattttacc cactattttg ctccaactat ttgtaatcgt acccactttt gaaataccga
    67861 gatgatcatt ttaattgata tgctgattcc caagcaaaac cccaagaaat taaatgctct
    67921 atcgcatcta gcttcttggg ttttgcttat ataaattcca acatttaaat tttttatgct
    67981 gttcggtctc agaaatgccg tttttgctta tatcacaata tcaagatgtc aaattattcg
    68041 tgttgccgat gactttgatt aatttttttc aaattgttta ttaagactaa tgctgtcttt
    68101 ttgcagatac caatgttcga actttttaac attatcatat gatcctgcaa gttttaactt
    68161 cttaccatta gatgttatca aagttataat taaccttgcc tttttcccat taggatcttt
    68221 tattttatga attttctgca ggtaatctct cctaatggca gctattttta cctcatcagt
    68281 gctgataaac cattgatcat ccacatacat actgcctgtt atgttgtgtt tttccatttc
    68341 ttccgagaac gaaaagttta aggctcgttc ttacttccaa agttgaatga gtccatggat
    68401 tggacttagt gtgagaattg agaacaacaa taagaatcca tatcctcccg caaacatccc
    68461 taaggctata ctgtctctcc atccataagt aggattagaa gagaaaagag tcaccgtttg
    68521 taataatatg aaaaggaata atccagcaca aacaccaaag ataacaactc caaacattga
    68581 atttttatac aatgaccatt tatttatagg cttttctttc attttacttc cccctactgt
    68641 tctgttcaca cggaatgaat tcaaagttca taagccctcc tctttgaaca tatgaatacg
    68701 acttgctaac tatactttaa aaaagcttgt tttaagcggt tataggcgag ttgtaatctc
    68761 tattcatctc attagcaaat aacttgccga aaagtctact gaaattaaag cagatagtgg
    68821 aagaattatt tagccaggat agacactatt tgtgaaacac atgtctttac ttttgaaatt
    68881 ctatgatgaa aagccttgga atacagtagc tcttaattgg taatactttt tccattataa
    68941 ataacctctt ttcattttat atttatcccc tctaaaaata atatctaatc taatatttta
    69001 aacttatatt tccctcgctg tgctaataga gcattactaa ataaatagaa actagaaaaa
    69061 agcattacgc ctgccagaat aaaccaatta tttgt
//
LOCUS       DAFEHS010000006        65756 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFEHS010000006
VERSION     DAFEHS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.006"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..552)
                     /locus_tag="LP327_000409"
     CDS             complement(<1..552)
                     /locus_tag="LP327_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1724557.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIF"
     gene            complement(697..2475)
                     /gene="uvrC"
                     /locus_tag="LP327_000410"
     CDS             complement(697..2475)
                     /gene="uvrC"
                     /locus_tag="LP327_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBL1724558.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDAFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(2657..2971)
                     /gene="trxA"
                     /locus_tag="LP327_000411"
     CDS             complement(2657..2971)
                     /gene="trxA"
                     /locus_tag="LP327_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBL1724559.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(3058..5418)
                     /locus_tag="LP327_000412"
     CDS             complement(3058..5418)
                     /locus_tag="LP327_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL1724560.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(5533..6081)
                     /locus_tag="LP327_000413"
     CDS             complement(5533..6081)
                     /locus_tag="LP327_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBL1724561.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(6139..6576)
                     /gene="zapA"
                     /locus_tag="LP327_000414"
     CDS             complement(6139..6576)
                     /gene="zapA"
                     /locus_tag="LP327_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBL1724562.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            6778..7695
                     /locus_tag="LP327_000415"
     CDS             6778..7695
                     /locus_tag="LP327_000415"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBL1724563.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(7951..8862)
                     /locus_tag="LP327_000416"
     CDS             complement(7951..8862)
                     /locus_tag="LP327_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL1724564.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(9265..9843)
                     /locus_tag="LP327_000417"
     CDS             complement(9265..9843)
                     /locus_tag="LP327_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL1724565.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(9863..10531)
                     /locus_tag="LP327_000418"
     CDS             complement(9863..10531)
                     /locus_tag="LP327_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1724566.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(10546..11616)
                     /locus_tag="LP327_000419"
     CDS             complement(10546..11616)
                     /locus_tag="LP327_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1724567.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(11787..13151)
                     /locus_tag="LP327_000420"
     CDS             complement(11787..13151)
                     /locus_tag="LP327_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL1724568.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(13148..14707)
                     /locus_tag="LP327_000421"
     CDS             complement(13148..14707)
                     /locus_tag="LP327_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1724569.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTDNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(14763..17243)
                     /locus_tag="LP327_000422"
     CDS             complement(14763..17243)
                     /locus_tag="LP327_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724570.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(17227..17562)
                     /locus_tag="LP327_000423"
     CDS             complement(17227..17562)
                     /locus_tag="LP327_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724571.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(17576..18610)
                     /locus_tag="LP327_000424"
     CDS             complement(17576..18610)
                     /locus_tag="LP327_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL1724572.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(18700..19380)
                     /locus_tag="LP327_000425"
     CDS             complement(18700..19380)
                     /locus_tag="LP327_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL1724573.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(19664..20767)
                     /locus_tag="LP327_000426"
     CDS             complement(19664..20767)
                     /locus_tag="LP327_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL1724574.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(21396..21866)
                     /locus_tag="LP327_000427"
     CDS             complement(21396..21866)
                     /locus_tag="LP327_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBL1724575.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            22196..23836
                     /locus_tag="LP327_000428"
     CDS             22196..23836
                     /locus_tag="LP327_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBL1724576.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            23990..25314
                     /locus_tag="LP327_000429"
                     /pseudo
     CDS             23990..25314
                     /locus_tag="LP327_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
     gene            complement(25382..25816)
                     /locus_tag="LP327_000430"
     CDS             complement(25382..25816)
                     /locus_tag="LP327_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1724577.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(25881..26717)
                     /locus_tag="LP327_000431"
     CDS             complement(25881..26717)
                     /locus_tag="LP327_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBL1724578.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(27459..28085)
                     /locus_tag="LP327_000432"
     CDS             complement(27459..28085)
                     /locus_tag="LP327_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724579.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(28380..28715)
                     /locus_tag="LP327_000433"
     CDS             complement(28380..28715)
                     /locus_tag="LP327_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL1724580.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(28702..28986)
                     /locus_tag="LP327_000434"
     CDS             complement(28702..28986)
                     /locus_tag="LP327_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL1724581.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(29042..30565)
                     /locus_tag="LP327_000435"
     CDS             complement(29042..30565)
                     /locus_tag="LP327_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL1724582.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(30558..31733)
                     /locus_tag="LP327_000436"
     CDS             complement(30558..31733)
                     /locus_tag="LP327_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL1724583.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(31737..31922)
                     /locus_tag="LP327_000437"
     CDS             complement(31737..31922)
                     /locus_tag="LP327_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724584.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(31888..33582)
                     /locus_tag="LP327_000438"
     CDS             complement(31888..33582)
                     /locus_tag="LP327_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL1724585.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(33579..34052)
                     /locus_tag="LP327_000439"
     CDS             complement(33579..34052)
                     /locus_tag="LP327_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBL1724586.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(34121..34276)
                     /locus_tag="LP327_000440"
     CDS             complement(34121..34276)
                     /locus_tag="LP327_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HBL1724587.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(34410..34790)
                     /locus_tag="LP327_000441"
     CDS             complement(34410..34790)
                     /locus_tag="LP327_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL1724588.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(34794..35009)
                     /locus_tag="LP327_000442"
     CDS             complement(34794..35009)
                     /locus_tag="LP327_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724589.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(35012..35419)
                     /locus_tag="LP327_000443"
     CDS             complement(35012..35419)
                     /locus_tag="LP327_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HBL1724590.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(35685..37160)
                     /locus_tag="LP327_000444"
     CDS             complement(35685..37160)
                     /locus_tag="LP327_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBL1724591.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(37150..37998)
                     /locus_tag="LP327_000445"
     CDS             complement(37150..37998)
                     /locus_tag="LP327_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HBL1724592.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(38035..38397)
                     /locus_tag="LP327_000446"
     CDS             complement(38035..38397)
                     /locus_tag="LP327_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724593.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(38398..38556)
                     /locus_tag="LP327_000447"
     CDS             complement(38398..38556)
                     /locus_tag="LP327_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724594.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(38697..39321)
                     /locus_tag="LP327_000448"
                     /pseudo
     CDS             complement(38697..39321)
                     /locus_tag="LP327_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(39318..39629)
                     /locus_tag="LP327_000449"
     CDS             complement(39318..39629)
                     /locus_tag="LP327_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724595.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(39673..39966)
                     /locus_tag="LP327_000450"
     CDS             complement(39673..39966)
                     /locus_tag="LP327_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724596.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            40160..40807
                     /locus_tag="LP327_000451"
     CDS             40160..40807
                     /locus_tag="LP327_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1724597.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            40868..42013
                     /locus_tag="LP327_000452"
     CDS             40868..42013
                     /locus_tag="LP327_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL1724598.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(42094..42399)
                     /locus_tag="LP327_000453"
     CDS             complement(42094..42399)
                     /locus_tag="LP327_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBL1724599.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(42424..42855)
                     /gene="ruvX"
                     /locus_tag="LP327_000454"
     CDS             complement(42424..42855)
                     /gene="ruvX"
                     /locus_tag="LP327_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBL1724600.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(42852..43118)
                     /locus_tag="LP327_000455"
     CDS             complement(42852..43118)
                     /locus_tag="LP327_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBL1724601.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(43373..44098)
                     /locus_tag="LP327_000456"
     CDS             complement(43373..44098)
                     /locus_tag="LP327_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL1724602.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(44459..44529)
                     /locus_tag="LP327_000457"
     tRNA            complement(44459..44529)
                     /locus_tag="LP327_000457"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44495..44497),aa:Gly,seq:ccc)
     gene            44646..45398
                     /locus_tag="LP327_000458"
     CDS             44646..45398
                     /locus_tag="LP327_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL1724603.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            45414..45893
                     /locus_tag="LP327_000459"
     CDS             45414..45893
                     /locus_tag="LP327_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL1724604.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(46144..47724)
                     /locus_tag="LP327_000460"
     CDS             complement(46144..47724)
                     /locus_tag="LP327_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBL1724605.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(47717..48460)
                     /locus_tag="LP327_000461"
     CDS             complement(47717..48460)
                     /locus_tag="LP327_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1724606.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(48636..49412)
                     /locus_tag="LP327_000462"
     CDS             complement(48636..49412)
                     /locus_tag="LP327_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1724607.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(49660..50496)
                     /locus_tag="LP327_000463"
     CDS             complement(49660..50496)
                     /locus_tag="LP327_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBL1724608.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(50507..51436)
                     /locus_tag="LP327_000464"
     CDS             complement(50507..51436)
                     /locus_tag="LP327_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBL1724609.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            51628..51972
                     /locus_tag="LP327_000465"
     CDS             51628..51972
                     /locus_tag="LP327_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL1724610.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIF"
     gene            complement(52055..52711)
                     /locus_tag="LP327_000466"
     CDS             complement(52055..52711)
                     /locus_tag="LP327_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL1724611.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            52937..54871
                     /locus_tag="LP327_000467"
     CDS             52937..54871
                     /locus_tag="LP327_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL1724612.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(54957..56180)
                     /locus_tag="LP327_000468"
     CDS             complement(54957..56180)
                     /locus_tag="LP327_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL1724613.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(56174..58072)
                     /locus_tag="LP327_000469"
     CDS             complement(56174..58072)
                     /locus_tag="LP327_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBL1724614.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(58066..59184)
                     /locus_tag="LP327_000470"
     CDS             complement(58066..59184)
                     /locus_tag="LP327_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL1724615.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(59267..60100)
                     /locus_tag="LP327_000471"
     CDS             complement(59267..60100)
                     /locus_tag="LP327_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL1724616.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(60093..61010)
                     /locus_tag="LP327_000472"
     CDS             complement(60093..61010)
                     /locus_tag="LP327_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1724617.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(61237..62343)
                     /locus_tag="LP327_000473"
     CDS             complement(61237..62343)
                     /locus_tag="LP327_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL1724618.1"
                     /translation="MVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATTLEVYVA
                     NGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPDIISMAD
                     TSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWASDFTPAL
                     CYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFINMLAQQ
                     NQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMFVAGGWY
                     ATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQQFLTFM
                     LTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            62543..63451
                     /locus_tag="LP327_000474"
     CDS             62543..63451
                     /locus_tag="LP327_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1724619.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            63592..65205
                     /locus_tag="LP327_000475"
     CDS             63592..65205
                     /locus_tag="LP327_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL1724620.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        19493 a        13344 c        11051 g        21868 t
ORIGIN      
        1 aaaaatgaca catgaaccat gttttttcaa ttccaatatt ccatctttta gcaattcagc
       61 atttaccgga tctaacccgc tgaatggctc atccaaaatg atcaatttag gttcatggat
      121 taacgtggcg atcagctgga ctttttgctg atttccttta gacaaagatt ttactttatc
      181 cgtctttttc ccttttacct gaaatttttc catccaaaaa tcgatttttg gttcaatctc
      241 ttttcttgat ttccctctaa gttctgcaaa ataaagcagt tggtcttgaa tcgttacttt
      301 cggataaagt ccccgttcct ccggaagata accgatgaca tcataatctt tctccccaag
      361 atgatggcca ttccataaca cttctccttg gtcttgtgtc aaaaaatcca agatcaaacg
      421 aaaagtcgtt gtcttcccag ccccgttttg gccaattagc cccatgattt ttccatcggg
      481 gatttggaaa gagacatgat cgacagctgt gtagttccca aaagttttaa caagatcctt
      541 gacttctaac atttcttcac tccttcttca agaaatcaca tatatcctta gacaccagac
      601 gtaagcgatt tattatttac caacaaaacg taaacgctct ttttgttgta cataaaatga
      661 taggacactt tactttcttt gtaaagtgcc ctttcctcat tcctgatgta atttatcaaa
      721 tacgttttgt gcgacatttt tcggcagtcc gatttcttgt aattcttctg tcgtggctgc
      781 tgtgatattt ttcaacgatt tgaactcttt aagcagcatt ttttttcgtt ttggtcctaa
      841 cccttcaatt tcatctaatc gggaagcaaa actgttttta ctgcgtagct gccgatggaa
      901 agtgattgcg aatcgatgga cttcatcttg gattcgctgg agtaagaaaa actctggtga
      961 atttcgctca agcggtacta cagccaaatc tataccgaat agcaactcat ttgttttatg
     1021 tttatcgttt tttgccatgc cagcaatagg aatatcgatt cctaattgat tttctaagac
     1081 atcttttgcc acatctactt gtccttttcc tccatcgatc aagatcaaat caggtaaagg
     1141 caacccttcg cgaatcactc gtgaatatct gcgatagatg acttctctca ttgatgcata
     1201 atcatccggt ccgttgactg tttttatttt atatttccga tattcttttc ttgccggttt
     1261 tccatcaatg aatacgacca tcgcagccac aggattcgtc cccatgatat tagagttatc
     1321 aaaagactcg atacgtattg gggtcgggat attcatcgcg tttcctaaac gttcaactgc
     1381 tccgatcgtc cgttcctgtt ttctggcaat cagatcaaac cgttcattca aagcaactga
     1441 ggcattctta ttcgccaatt caactaattt tttcttttct cctcgttttg gttgcagcac
     1501 tttcgtagct aacaaagctt ctacactggg cttgtcgatc gtatcaggaa tcaagacttc
     1561 tttaggaatg aaatgctcat tttcctgata aaactgaccg aggtaagtca agaaatcttc
     1621 ttctgcttcg ttatagaaag gaaacattga aacatctcgt tcaattagct ttccttgtct
     1681 tacgaaaaac acttggacac acatccatcc tttgtcaatc gcataaccaa atgcatctcg
     1741 atccagtaaa tcagtatttg tcattttttg gcgagtcatg attgtctcaa tggcacggat
     1801 ctggtctcgg tattctgcag ctttttcaaa ctccatgttt tcagcagctt tttccatttt
     1861 ctgatggatc tctgactcga ttttttcgtg tcctccgttt aaaaactttt tcacttcatc
     1921 aacgattttc gtatagactt tcggatctac gtcgaaataa taaggacaca gacattgtcc
     1981 caaatgatag taaagacaag gctccttcgt ctgactaggt ttgcattttc tcagaggaaa
     2041 taatcgatcg aggatcttct ttgtctcatt cgccgcaccg acatcgggat atggtccaaa
     2101 atacagtgct ttgtctttta gtactttacg agtaatcacc aaacggggat atttttcatt
     2161 cgttatctta aggaaaggat aggttttatc atcctttaac ataatgttgt atttaggatc
     2221 atttttcttt atcagattga tctctaagag caatgcttcg atattcgatt ctgtaacgat
     2281 gtattcaaag tcttcgattt cactaacaag ccgttccgtt ttcgtattat ggctgccagt
     2341 aaaatatgag cggacacgat tttttaatat tttggctttg ccgacataaa tgatcgttcc
     2401 atttttatct ttcatcaagt aacagccggg ctgatcagga agtaaagcta atttgttttt
     2461 gattcgttcg ttcatctggt actccccttc atattcaatt aaagttatta tagcatgtct
     2521 gtcaaaaaaa caatccagcc tgacaacgta attatactca aaaaatatta atttgaataa
     2581 ccttaactca accgcaaaaa aaccgaggac ggaactggat tcagtcccac cctcagctta
     2641 tttttgctgt aagtttttat aaatattgat cgatcatttg acgtagttgt tcttttgaat
     2701 gaacaccaac agctttttct actacttgac catctttttt caacattaat gttggaatgc
     2761 tcatgatacc gaattgttga ggagtttctg gattttcatc cacatccatt ttagcaattc
     2821 tgaattcttc ttcatcatat tcttgttcca actgatccaa aatcggtgct tgcatacgac
     2881 atggaccaca ccaagttgcc caaaaatcaa tcaatacaag tcctttatct gtttcttcat
     2941 taaatgtctt atctgtaatt acttgtgaca tatcaaagac ctccattttt aaaatctatg
     3001 attagtatag catttctata cttggatagc gaaccatctg cttaccttta gtaagcttta
     3061 cttaaattta acaattgtcg caccatttcc gccttggttt gccggtgcaa actcaaagct
     3121 tttgacgctc cgatggtttt taagataatc agtaattccc ttgcgaagtg cgcctgttcc
     3181 ttttccgtgg acaatagtca cttgaggata accagctaga atggcagaat ccaaatattg
     3241 atctacctca cttagcgctt cttcgtatcg ttttcctcga aggtccaatt gattcggcac
     3301 gtgactacta gattcagatc gtacagcatg gataactcgt tgtgtcggct ctttttgagg
     3361 agcaacaggc gtcagctcgt cttcggacac attcattttt aatataccga tttctacttg
     3421 ccactgattc ccgtttttac gtaaaagcgt ccctctttgg ccgtaagtcg taaccaacac
     3481 ttcgtctccc ggtttcaacg ttttttgttc tttcgccttc ttcaatactt tatttttctt
     3541 caaagtttct tcttgatgta gatttgctaa ttgggtcttt gcatcgatca actgatgctc
     3601 tttcacattt ccttgcccaa tctgctgttg cattttacga atatcagcaa tgattttttc
     3661 tgctttttct tccgcctctg acacgacttc gtttgctttt ttctttgctt tttccatttc
     3721 tttttcacgt tcttcaaaga aatagctgta agcttctttc aaatcatcgt gaagttcttg
     3781 tgcctcagaa acaaaatgac gcatttccag atattcggtc tctgccattt tccggcgatt
     3841 ctctaaatct gtgatcattt cgtttaaatc ttggctctca tcattcatca actgcttcgc
     3901 ttcatcaata acttctgtat caagacctaa tcggctagag atttcaaacg cattgcttcg
     3961 tccaggaacg ccaatcagca aacgatacgt tggacttagt gtatccacat cgaactccat
     4021 gctggcatta atcgtatttg cccgattgta tccataaact ttcaattcag gataatgcgt
     4081 tgttgccatc acataagcag actttttacc aagatcatct aagattgcga ttgctagagc
     4141 agcaccttct tgcggatctg ttcctgcacc gagctcgtca aataatacca gacttttttc
     4201 attgactttt gataaaatat ctacagtgtt cgtcatatga gaagaaaagg tcgatagact
     4261 ttgctcaatc gactgttcgt ctcctatatc cgcaaatact tcctcaaaaa taccgatttg
     4321 actttcttca cctgcaggaa tcggtaatcc tgcttgcccc atcagttgta acagacctaa
     4381 agtcttcaac gtgatcgttt tcccacctgt gttaggtcct gtaatgacga tggcttgata
     4441 gtcttttccg attgtgatgt cattagaaac tactttttcc tgatcgatca gtggatgtct
     4501 tgcttgtttc aagacgacat gattgtcttc actgattccc ggcacgatgg cttttacttc
     4561 tttaccaaat cgtgcttttg cattcatcag atccatttta ccaatcacat aagcattgtg
     4621 gatgatttct tgacgatggg gaacaagttc agcagaaagt tcacttaaaa tacgttgaat
     4681 ctcatttcgt tcagcgattt ggtattgacg caaacgatta ttcaaatcca ccacttgttt
     4741 tggttcaata aataaagtct gtcctgaagc actttgatcg tggactacac cgccaaatac
     4801 accgcggtat tcttgtttga ctgggataac gtagcggtca tttcgcatgg tgatgatcgc
     4861 atcacttaaa tactttgcat ttttcccacg gacgatccca tctaactgtt cacgaacagc
     4921 ttgttcactt cgccgaatgt tctgtcttat gctctttaac tcaggagaag cgtcatcagt
     4981 cacgcgtccg tcttcatcga ttgcttcctt taaacggcgg ctcaaaacag ggatcgtgac
     5041 taactgatct gcccacatat acaaacgtgc aaattcgatt tcactgtcag aaagatcatc
     5101 aatgaaacga ttcaattcgc ttgtcgtaga caatactctg gaaacttgtg caagctccac
     5161 tccattcaag tctgctccga tctcgatccg tttcatatgg ggacggatat tttcaagttt
     5221 aggtataggg atcccgcctc gcagacgttg cactttcaat ccatcctcag tttcttgaag
     5281 ccagtttgtg atcgtttgtt tctcactcac tggtaccaat tctgccagtt cttcttttcc
     5341 ttgtgcagtc actacaaatt gtctgaccat ctgtttgact ttttcaaatt ccaatgtttc
     5401 aaggattcgg ttattcattt tatcacctta tctttcacta tgaaaaaaga gctgtgccgg
     5461 acatacgcta cgcagccttc ttaaataaac tactctgtac aaaaaacgaa atattttcca
     5521 gcactatttt ccttaattca aaatattcgt tacccaaagc tcatacatct tgttggtaag
     5581 aaatggcgtt ctctcaacga taaaacgagc gaaactatgc tcgccgaatt gattttggac
     5641 cacatctaat ggaacaaagg caagtaactg caaaatcaaa aatatagtaa cgtaagccat
     5701 gattagtgac aggatgcctc ctgcaatcca gtctacctgt ctcaagatcg gcacatacgt
     5761 caatccatgg acaaaaaccc ccacaaatcg ggtaagcagc cagccaatta gcaaaataat
     5821 cagaaaagca gttcctgcat aaaaagcttc atctaggtga aaagcaaata cctgatcaaa
     5881 aaatgctaac tttgatgttg gggtgacagc tgggtatgga atgtataatt ccaaatgact
     5941 tgctaatggt ttgtaaaaat gctgggcagc aataaatgac aatacatatc caatcgcgaa
     6001 tatgatttgc aaagcaaacc cgcgacgtgc tccagaaaaa aacgcaatta gcaataaaaa
     6061 taaaatcagc aaactcagca tgctacgttc ctttctcttc taactttttt aaatttttct
     6121 tattttgata aaaggatctt aattagaaga tttttgttta atttgtcctt ttcgtttctc
     6181 gtttaaaatc tgttgtgctt ccacatgatt tttaatggta tgatctgctt gaccattttc
     6241 tttcaatact tcccgagctt cgttctcgat tgcttcaatg cgtttcacac gattttcaag
     6301 ctctgtaaat ttgatcattt ttttctttaa ttctgctgtt tcttcttcca gctccaagat
     6361 tctttcttgt ttcttcaatt gatccgaaag cgcattgaca gccatcaaga ttgctgcttg
     6421 ttcattatcc atttgtggtg ataattgttt caattctgct aactgttcat tgatcagctt
     6481 cgttacgata tccatatgat gcttggtttc ccgtccgata atagtatatg tttggttggc
     6541 aatcactgct ttgtagcgtg ttttttcgtg cgccatacga atcatttcct cctatttgat
     6601 gaaagtttta tttagtgtac catgaagttg gttctttcac cgttctccac aaatattttt
     6661 cacatactta actattatat gataaacttt aaaaaaatgc tatgtctcgc aatctcttag
     6721 aaaacgaaaa gtcgtgagtt gcggtatagt tttatgtata aaaacgagga gaattttatg
     6781 agcaacgtca ttatcaaagc aacaaacgac caacttagtc aaatgaaagc atactatcgc
     6841 tcttatttgt taaataaaca gattccttat actagctttg ctgcaaaaaa agacgggacg
     6901 accattaccg cttatacatc cggaaaagtg atgtttcaag gtacgaatgc agcaatagaa
     6961 gctgataaat ggggggaggc acaggataaa caagccaagc cagcaaaaac agcctcttct
     7021 ttgccaaaaa atatcagtca attatccgtt ttaggtagtg atgaggtcgg aaatggcagc
     7081 tattttggtc cgctaacagt atgtgctgct tatgtgaaac gtgatcagct tgcagaatta
     7141 cgtaaacttg gtgttagaga ttcaaaagag ttaaaagata gccaaatcat ccagcttgct
     7201 gacatcttaa aaaaaaccat cccctataaa ctgcttgtct tatctccaga aaaatacaat
     7261 gagatccaac caaaatacaa tgccgttcgg atgaaagtcg ctttgcacaa tcaagcgatc
     7321 catttgctgc taaaaaaaat cgagccagaa aaaccagaag cgattttgat cgatcagttt
     7381 acgccggaag caaattttaa aaaatatgcg cgtttagaaa aaaatcaatt acaagaaaaa
     7441 ctgtacttcg ttactaaagg agaacaatat catttagctg ttgctgctgc ttctatcatc
     7501 agtcgtgcca gctttttgga agagttacac aaagcatcag ctgaagctgg aatcaccctt
     7561 ccatcaggag ctggaacgaa atctgaccaa gtagcggcaa aactattgga gaaaggcggc
     7621 atgcctatgc ttgaaaaata tgccaaactt cattttgcca atacagaaaa agctttgaag
     7681 ctgctaaaaa aataatggaa ctgtgacaaa agtcatgtca cagtcccttg ttccgaataa
     7741 acggtgttct catgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc
     7801 tgttttttga aattgctcct tgtgacgcgg agttaaacgg caggctcaca acctcttcct
     7861 taaacggtgt tcaaaagctt gcatctgcga taataagccc ggccaaacga aaaatatgag
     7921 gagctatttt tgtttggctg ggcttattta ttactgaaaa taagtggtca atgcttgtgt
     7981 caaacttgca gcaatctgct gctgataact gtctgttact aattccttta aatcactatc
     8041 attattcata taacccatct cgattaaaat agaaggttga agattttctc gaagaacttg
     8101 atagttccca gtggagattc ctcttgaatc cagaggcaga tcagtgaagg agtcagcaat
     8161 tgtctgtgct aatgtttcct cttgtccata gtaataatat gtcgtgattc cagttgcttc
     8221 attcgcatat tccgtagagt ctgcgtgtaa actgataaag acatctgcct ttttttcttc
     8281 actgtagtcg catatatctc caagctgtac taattcatct gtagtatggg tcaaataaac
     8341 cgttgctcca gcatcctcta gcgccttttt tacttttatg gctgtactta atgttatatc
     8401 tttttcttct gtttgttcat cattgcttaa tgccccagta tcatttccgc catgcccagg
     8461 atctaataca atcactgcat cagatagctc ttccggtttt gtttgtatat tctttgtgac
     8521 tttggttcca tcttcgaggg ctaaatatcc agtatagcca tcatttgtct tgatctgata
     8581 agcatttttt gtttcagaaa gaaactccgc cagctctccg ccgtttatag ttgcaagtgg
     8641 ggtagttagc gagtcatctg tatatagtgt acggtcattt tttccgattt tgattaatgt
     8701 ctcttgttct gtatcatcaa atgattcctt tgtctttgta accgtactgt tcgtactgct
     8761 gaccgtttgt ccaactatct ttttttgacc aaaaacatct atttgaaaac agataataat
     8821 gataatgatt gcaaatacaa aagctccaat tatttttttc attcttgact ctcacctcgt
     8881 agatccagac taagggtcaa aacttaaaat cagcttaagt acgcaaaaaa ggggctgtaa
     8941 aagatataac cgtagaaaat aacattactg ctaaaatgat ttttttcatt tttggcatat
     9001 cctgaaatct ccttttgact attcgtggtg attcatctac ccaataaata gtttccttca
     9061 ttctacaaac aattaatttc ttcgttatta aattaaaaca cttcctggaa caccgacata
     9121 ataaataaaa atataccctg ataatcatga gagaataaca aatttgtatt tatgaaaaac
     9181 tacctatata aaaaaaacaa gatataacga tcttcgtcat atcttgcttc ttcacctaac
     9241 tatttttctc cctatataac agctttacgc ttctcctaat acaccattca aatataaatc
     9301 gatcaattgc gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg
     9361 tgctaacact aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac
     9421 agattggttt gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa
     9481 gaaaagatga tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa
     9541 caaatcgacc agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa
     9601 tcccgtttcg cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt
     9661 ttctaaaact gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggttg
     9721 ggtgatccca actttttgtg cgatctctct tgttgaagtg ccgagatatc ccttggacat
     9781 aaacaagtca gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac
     9841 catttttcca cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa
     9901 atattctatc gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat
     9961 ttttgtcttt tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca
    10021 aactagcagt tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga
    10081 ttgcgaccct ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat
    10141 ccacgccaag ctgctcaaat aattcttttg cttctttttg acgatgttca tgtttgatct
    10201 tatctactaa taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca
    10261 aaataaagcc aatttcttta aaacgtagtt taacgcgttc actttctttt tgattactga
    10321 attcttgatc attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag
    10381 tcaaaaaagt acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat
    10441 cgatactcat ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt
    10501 tttctacttc tttaaattct attgctttca tgctcttcct ccttcttatc caattgcctt
    10561 caacggatcg atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac
    10621 cagcatcaac aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc
    10681 aggtagaaca agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga
    10741 taaaatgaat gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc
    10801 tttcattaca ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat
    10861 cacaatagcc gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc
    10921 attatatcca ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg
    10981 taaattactg attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc
    11041 ttctttttcg aagcggatct cttggtaagc attgatggtt gtatagagaa ctggtgagac
    11101 attgaatttg gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa
    11161 cttaactgta tctcctacgg ctagaccata ttcttctttc aaactaatat cgcctactgc
    11221 ctcatcatcg ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt
    11281 atcgataccg aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac
    11341 gactccaggt gtctgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt
    11401 cttagtaatc atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc
    11461 tgcctcccac ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt
    11521 caaaaaaaat accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa
    11581 ctttgaatgc ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc
    11641 attttataca acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt
    11701 cttccgctat ttcattatat gaatttttta ttctcgattg attaatattt attatatccc
    11761 cacacaataa aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct
    11821 ctcttagcta gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtgttca
    11881 gacagaaatc caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata
    11941 tccgcaagca ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt
    12001 atttctaatg tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcatcg
    12061 tattgtggaa atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc
    12121 gcttcatctt ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt
    12181 atcttttgtc tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt
    12241 gccatatgta cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa
    12301 ctgactggga cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca
    12361 ttgactatca cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta
    12421 ggacgagttt gggcagaaaa aatgaccata tctgccttta ttgcttgaga tagaagttgt
    12481 acggatacta ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa
    12541 ttgatttgct tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat
    12601 cttgataaaa cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa
    12661 gcatctgcta attccaatcc aatataacca gcaccgaaga tcactacttc ttttgcttct
    12721 tttaaagcgg caagtacttg cttagattgc actagatttt tataggtgta gacattttca
    12781 gcttcaattt gtttatacaa ggtgacagga gaggaacctg ttgctacgat taatttatca
    12841 taggttatgg tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga
    12901 tcaagttcag ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc
    12961 atgttatggg caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca
    13021 tttgaaacat agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc
    13081 ggttgaagtt ttcttagatt tagggcagct gtgatgcctg catgggaagc accaatgatc
    13141 acgattttca tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa
    13201 attggttcaa ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag
    13261 cggacttctg tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt
    13321 ctttctcgta ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt
    13381 ctttcacgat atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta
    13441 attctaactg cttgatcagc gtccgcaaaa tagatgcata gcgagcaaat aattcaaagc
    13501 gggcattttc ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc
    13561 tttcacataa aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt
    13621 cttggccaaa aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca
    13681 ctgtcaacgc aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt
    13741 ctatcgattg tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc
    13801 ttatcaaaaa ctcctggaca tgcgagttat ctgtatctaa tgtaaagtta ctgatcatga
    13861 aaagataaaa aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac
    13921 gggtcaaaaa tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa
    13981 gattgctata atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa
    14041 gccatatttc aaatttcaat cgtccttctt ctgtaatagt aaaactaaac tctttttgca
    14101 gcagatctaa aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagataag
    14161 gacgtccaaa ccaaaataat tggaaataaa aataacaaat ttgactttct ggaccggtca
    14221 gccgcccgct ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc
    14281 tgcgagacaa agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg
    14341 agccattttc caacagataa agaagtatct gatattttgt agaatagcgc aagtagcaat
    14401 gataataaac atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt
    14461 ggataacaat ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt
    14521 ctttcaaggt agcccgatca actgataagc gggtaagtac gttattaacg gaaatctctc
    14581 ctttttcctc taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca
    14641 aactgaaaag ttccaagtcc actcctccaa gcatttctga ttttaacagc agaagtttcc
    14701 ttctcatcct tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc
    14761 acttagctgg ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca
    14821 ggttgagcat tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca
    14881 acgagaacct ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc
    14941 tgaatcaatt gttttgatcc agaatgattg aagtataatt ttgttccgct taattcttga
    15001 ccttgatcat tcttaaataa agaagtcaat gtccctgtca gttgccaagg agacgtgctt
    15061 ggaagccgag tatcccgcac atcaaaagaa aaatcgcctt ttgcttcata ataacttgtc
    15121 ttcaacggtt tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt
    15181 aattcttgtt tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc
    15241 ttagcaccat ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg
    15301 acggattgta cagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta
    15361 tctcctattt tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct
    15421 gcgatagttg taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt
    15481 gagatcgtga tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca
    15541 ttttcttgaa tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg
    15601 ctaaggtttt ttagctgact atctgcactc acagatggaa aattagccac tgttatttga
    15661 ggaagagtgg tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg
    15721 ttagtaagta catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca
    15781 ggtagtgtag aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt
    15841 tgatctgtcc atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc
    15901 gaaccctttt ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa
    15961 aggagctttc cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc
    16021 ttgtactgct ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc
    16081 ccactgtaca cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc
    16141 agtattgctc cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga
    16201 ctaaatggca actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg
    16261 gtagagcctg gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta
    16321 ttatattttc ctgttgaatc acttgttgag atgtaactat tccacgtatt tatctctgca
    16381 cttgcagaaa atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat
    16441 ctacctgcag ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa
    16501 tcataaaaat caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg
    16561 gtcaaaattt tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca
    16621 ttagctgtaa catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata
    16681 atagcaataa tatctgccaa tagagtattt gtatcccacg accccatccc aaaacgcaag
    16741 gtatggcttc ctgctgataa taaactaccg ctagtaacct tgatgggggc accggcaata
    16801 tacaagtagg cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc
    16861 ggcaaattga tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt
    16921 acatttgtta gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga
    16981 tcatttgaac catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt
    17041 agttggacaa gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt
    17101 tcatacgtgt atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa
    17161 ggtgaaaaga taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat
    17221 gaaaatttat tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa
    17281 ccacgcaaga agccataatt ctataccaat gatcaaataa agaaaacgga tcttttcttc
    17341 attcgtctcc gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg
    17401 ctgttttgat gcgccattga ctgaaccact tggatgcgga actggactta gatcataaaa
    17461 tgtaatcgta cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag
    17521 caaacttgca ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg
    17581 ttgtttctcc ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca
    17641 ctagaactgc agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa
    17701 aaacggaatt ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa
    17761 atcgccattc attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt
    17821 ttgctttatc accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa
    17881 attcattaga cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata
    17941 tcaaattgct gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg
    18001 cgttgacctc gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac
    18061 tttcttgtaa aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg
    18121 tagcattttc tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc
    18181 ccttaaagct ttttgctggg attttcactt ctataggtat agttgtttct gcatgtgctg
    18241 atacattgat ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct
    18301 catctaacgt tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg
    18361 gattgacaga gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta
    18421 gtgtctgatt ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt
    18481 ggttctctgg aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga
    18541 ttgacggaaa aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac
    18601 tttgattcat tgtccaccac atcctttcaa caacaactgc ggtaaaaaat ccttcaccta
    18661 tagtattttc accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac
    18721 gaggttccaa gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt
    18781 gatatttgct ggatcataaa cagaaagcca tgttcccaga ccttgatctt ttgctgctgt
    18841 aaatattggt gtcgctgcac ttgctttact caacccagtt acagtttgac tagttggtcc
    18901 agtggattcg ttatctgacg tacttttaac agtggatgtt ccaaaatcta attcagcgcc
    18961 ttgcaaaact tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg
    19021 tgcttggcca tctgcaccta cacctcgacg atctgatact tggagataag gtgtatcgtt
    19081 ttttgaagcg tctacttgat acgtttttgt tccgctttca atctcgtgtg taccaaatgg
    19141 caattcagga actacatcca atgttaaaga acctgtctgg ccagttgctg gattttccgg
    19201 atctgctggc tctactggtt ttgaaggatc agtagggtta agaggctcaa cggtatttgt
    19261 tggttcagta aaggtaatat ctccttttga agtcgcttga ccctcagcaa aagctggtgt
    19321 tgccaagccg cctagaagaa gtgttgaaag taataatgtc ccaatcatgt tctttttcat
    19381 aataaatcac tcctttttct tagtgaccta agtatagtag agacagtatg caaaaaatcc
    19441 tcatgaattc atccgcttac ttgaaattat gacccttttt gtataaaaga gaaaaaaaca
    19501 ataaaatcgt ttacatgaat attcttcctt ttttattaag tatgaaacaa tatttcaatt
    19561 ttattttttc gtttcaaatt attctattag tgacatcctt gaactataaa aataggagta
    19621 cggcatcatc tgccgcactc ctattttttt cgtcatttat ttcttaaata aaacgattct
    19681 cacgcaattt tatttttttg atatcttcta ctgtttgtgt tccagctaat tgcatgacca
    19741 tttccaattc tgttttaaag aaatcaaaca cttgatggac tcctgtactt cctcctaaag
    19801 acaatccata aataacgggt ctgccaatag caacaagatc ggcacctgat gcgattgctt
    19861 tgaaaacatg ttgtccacgt cgtacaccgc tgtcaaacac gattggtaca cgtttatcta
    19921 ctgcttctgc cacatattgc aacgaatcaa acgcagcagg ccccccatct aattgacggc
    19981 caccatggtt agatacccaa attccaccag caccagattc taatgatcga tagacatctt
    20041 cttctgattg aacacctttc acgtagactg gcaaatcaga atgagcagcg ataaattcta
    20101 catcttttgg actaagtttt tgtttggaag atttatatac agcatccatc gtttggccta
    20161 ctcctgattg ataagcctga acgatcggca ttggcagcgg gaaggtaaac ccgtttctgc
    20221 ggtctgtttc acggtttccg ccaactgttg catccgctgt caatacgatt gcttttgctc
    20281 cattacgctt ggccacttca agaatatcca aattgattcc attatctttg ctcatataaa
    20341 attgaaacca ttgcggtgct ttctccccgc ctgcttctct tatctcttct aaagtacaag
    20401 aagcgtaaga acttgctgta tagatcgtcc caaaatctgc tacacctttt gcagaagctt
    20461 tctccgcctt cacatgagct aatccatgag cagcaactgg tgccatgatg attggtgcag
    20521 tcagcatttc ttcgtcaaaa tgtgtagttg tatcgggtaa ttctacatct cttaatacat
    20581 gaggaataat caaacgatga ttgaatgccc gttcattttc ttgataagta aataagtcgc
    20641 cagcaccact gctgatgtaa ccataaccac cagctggaat cacttgtgaa gccgcgattt
    20701 ctaaatcttc catattgata aaatctacta ttccttcatt tgtacctgct tgatatgttt
    20761 tttccatctc aatgccttct ctctataaaa tttgaatgat gaacaatatg atagcgttta
    20821 cactttttat attactcttc gacattgata caggcaaata atctgtttac atttcagcca
    20881 ttccagatgt cactactctg gaagaaatga ttgtaataaa aatagataaa aaaccgatgg
    20941 gactttgaca aaagtagtgt caaagtccct ttttttgaat aaacggtgtt caaaagccat
    21001 tttctcggta ttaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt
    21061 cttactactc gaaactaagc gactttttca caacctctcg ataaaacggt gttcaatcag
    21121 ctgaccctcg gtattagctc aaaaaatggc aaaatatgag gaactatttt gccatttgtc
    21181 tctctaatac tcaggtcaga acgttgactt cacagtctct tccttaaacg gtgttcaaaa
    21241 acttgcatct gcggtaataa gcccaaccaa acgaaaaata ggaggagcta tttttgtttg
    21301 gctgttctta ttacttgatg cagggcaggt ttttcacgac ctctgattca cggtgtttct
    21361 ttttcatcaa catttttacg aaatactgtg gaagactatt gaaataagtg tggattgttt
    21421 ctttgtgttt caatcgtttc ttgccattct tcatttaatt ttggattacc gttttcttcc
    21481 ttgaactcat aaggtcctaa ttcaaaaatc ccagacagta tttgattcac gatttcatat
    21541 agttcagtgt gatcagacac atgattcttg aagatcatct cattatcacc acgtttgaac
    21601 ctcacattcg ggctctcatg atgaacctct atgcattttt ctcctttttt taactgacgg
    21661 acttctgaaa acatctcata aatatcttct ccagtaaaac cttcagcaag cgccagactg
    21721 tccacaagac cgcctactgt cagttcagag attattgctt gattggaatc taattttaat
    21781 gtatagcttg tcgcttctac tacgccacat gcattgcaac cgccttctga tagttcgctt
    21841 tttctaatca attgaacttc tttcattttc tcttcctcct cttaattttc tacttatgta
    21901 tttttatcac ctgacatagg tgactcctat caacgctatc atgtgctttt ttcctcagaa
    21961 atcctattta tccattccat ctatccctta ttcttctact tttctttctc tagcatataa
    22021 gagatacgca tagtattcaa gcaaggattg tcgatataaa aagaacaaat actgctattt
    22081 tgatttcttt tggctatatt acatataaat aaaatttatc atacctgctc tttttgacta
    22141 tatcatcatc tgacttctat acttcgtttg tattaaaact taggaggaat cattcatgct
    22201 tacagggtta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
    22261 agaacgggca aaatatggaa tcactggtat gcttccaagt actgtccaaa cactggaaca
    22321 acaatctgtt caagcttatg gtcaatactt aagcaaacaa acagatttgg aaaaacgtat
    22381 cttcttaatg aatctattca atacgaatcg gacattgttt tataaattga tgggcgaaca
    22441 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
    22501 caacgaaatt ttcttgaaac cgcaagatgc tgcatttctt tcaatcgacg aaccagaaaa
    22561 catcaaagca agtttgaaga acgcggcaga tggacgagac attcgtttga ttgttgtgac
    22621 tgatgcagaa ggaatcttag gtatgggcga ctggggtgtg aacggtgttg acatcgctat
    22681 cggcaaattg atggtctata ctgctgctgc tggtattaat ccagcacaag ttttgcctgt
    22741 atcgatcgac gctggtacta ataatgaaga attattgaat aatcccttat acttaggcaa
    22801 tcgtcatgct cgtgtagaag gcgaaactta ctatgaattc atcgatcaat tcgttcaatc
    22861 agctaccgaa ttattcccag aattgttatt gcattgggaa gattttggac gaggaaatgc
    22921 tgcagcgatc ttagaaaaat acgaagatca aatcactact ttcaacgacg atatccaagg
    22981 tacaggaatt gttgtcctag caggtgtatt aggcggattg aatatatccg gtgaacaatt
    23041 gaaagatcaa ataattttga catttggtgc tggtactgct ggtgtgggta tcgcaaacat
    23101 ccttttagat gagatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta
    23161 tcaagtagat aaacaaggtt tattgtttga agatacagaa ggattgactc ctggtcaaat
    23221 cccattcgct cgcaaacgct cagatttcac taatgctgat gaattgacaa acttagaagc
    23281 agttgtaaaa gcgatccatc cgacaatcat gattggtact tctacccaac ctggagcatt
    23341 cactgaagaa atcgtgaaag aaatggctgc tcatacacca cgtccgatca tcatgccttt
    23401 gtctaatcca acaaaattgg cagaagcaaa agcaaaagat ttaatagaat ggacggacgg
    23461 aaaagcatta gttggaacag gtatccctgc tgctgatgtt gaatataatg gtgtaactta
    23521 ccaaatcggt caagcaaata atgctttgat gtacccaggt ttaggacttg gtttgattgc
    23581 ttcaacagct acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg
    23641 aatcgttgat gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga
    23701 attttctcaa acgattgctg aaactgtcgc aaaatcagtt gtcgcacaaa acttgaatcg
    23761 tgaagaaatc acggatatca aagaagcagt tgaatctgct aaatgggttc ctgaatataa
    23821 atctttagaa gattaagatc gttatagtaa aagaacatac agaaactttg tatgacgtct
    23881 aaaaagaaaa aagcgactgc ggcaaaacag cggccagcca actcctgctc ttttgtcgct
    23941 tttctttggc ttttttcata tgatacagaa taagaaagga tctgtaaaaa tggcacaaaa
    24001 aacaacaact gcaactgcag ttgaggaaaa gaaaaaagaa ggtttttggt ccacaaaaat
    24061 caccggcgta agtttgccac tttatttgat catggtcctt gtcctgatca ttgcaatggc
    24121 tttagggaaa ttaccgacga atatgattgg agaccaatcg cagttttggt tattttcggt
    24181 aatttatttc attttatcgg gacaaaaatc cctatcgtca aaagttattt gggtggtggt
    24241 tccgtttttg cgatcttcgc ttctgctgcg attgctacat ttgggatctt acctgaatat
    24301 gtagtgaaat ccacagaaaa ctttgtcagc aatatgggct tttgggactt ttatattgct
    24361 gccttgatta ctggtagcat ccttggtatg aaccgtaatc tattgatgaa agcttctgtt
    24421 cgttttattc ccgttgcact tatttcaatg gtcgtctcct tttttgccgt aggactcgtg
    24481 ggcatgctta tcggaaatgg ttttgcaaat tccgttttat acatctcatt acctgcgatg
    24541 gctggtggtg ttggagccgg agccgttcct ttatcaacga tttatgcaaa tgttttaggt
    24601 acggatgctt cttctatcat ttcccgcctg attcctgcga cagcaatgac aaatgtctta
    24661 gctatcatcg gtgcagcatt agttgctcgt gctggacaat caatgccaaa attcaacggc
    24721 aatggtaaat taatggaaaa aggcgatctt ggcgatggaa aacctcgaac tgtgaaacct
    24781 tccattccac agttaggtgt tggtttgatg gtatcattga ctttcttcat cttaggacaa
    24841 attggtaact attttgttcc taaagtccat gcttacgcat ttatgattat tatcgttgtc
    24901 atctgtaaaa tcgccaacct attaccagag tattatgaag atgcagctat catgttcaat
    24961 aacttgattg tcaaaaactt gacagcagct gttcttgccg gtatcggtat tgcgttgctg
    25021 aatctgaatg tattagcttc tgctttgaca tggcaatttg ttgtcttatg tctgacaagt
    25081 gttatcgcga tttcagttgt ttcaggattt gtcggtcgtt tgtttggttt atatccaatt
    25141 gaatcaacta ttactgctgg cttatgcaac aactcgatgg gcggaacagg aaatgtagcc
    25201 gtcttatctg cagctaatcg gatggaactt attgcttttg ctcaaatggg caatcgactt
    25261 ggtggtgcca tcgttttgat catctctggt ttcttgatgc aattactttc ttagtcaaaa
    25321 aaacaaaaat acctgatcat caagtatcgg cttggtgatc gggtattttt ttgataaaga
    25381 tttacaggat tttcaaaaat cttttttgca ttttatcgga atgataaccg atgtgttcat
    25441 aaaattggtg tgcttctact ctgtattctg cagaattaag ccttattgtc gttattcctc
    25501 gttttttcgc ttcttcttct acgccagtca tcaaagcctt tccgatgcct cttttttcag
    25561 catatttaga cacagcaagt gccataatat tgaacattgg atcgctaaaa atcgattcat
    25621 aggactccgc ttgtacatag ccagctgctt ttttagaagt aggatcttca taaaccaaga
    25681 aaaaattttt ctctggctcc ttcagcagtt tcttcatttg tctcaccgta acggttaatg
    25741 gtatatcata ccctaattga tccgcattga tttcacgtag tgcttctgca tcttctaaac
    25801 gcatttcccg taacatccaa atcaccagcc aatttctgat tttttaaaat gaattttttt
    25861 atcatagtat actctttttt tcatttagaa caatatgttt cagtgatttt gacttgcgtt
    25921 agtctagatt ctcctaatgt ttgtaagatg acaaaggcat tgtaacaatt gaccaaaaga
    25981 accacgagga caattacttg aaaaataaag gtcacttttt ttggagacaa aaccctgcta
    26041 aatcttgctc cccacaaacc tccaataaca gcagcgggga taacataaaa caacatagat
    26101 aaatcaaagc gcataaagct acttgttaaa gcaatcgtta ccaattttgc cagttgagaa
    26161 aagaaaatcg tagatagaga atatagtgtt gcttctttta ttggcaatgc aaacatcagc
    26221 atcaacaaag aaacattgac tggtccacca ccaataccta aaaaactagc taaaaagccc
    26281 agaacccccc cacatataag gtaccaagtc catgacgtca aatgaaattt tggctgatga
    26341 tgcttggtgt agaagaacgc aaaaaccaag gtcaataccg tcaaaaaaat ttggatcagc
    26401 tgcacatgct tctcgttttc aaataactgt aaaaaaactt cgaatacaat gttgccgact
    26461 actcctccaa caacagctcc actagatacc caaccgacaa tctgccaatt cagtgatttc
    26521 ccgcttgata cttgtcgcac agtcgaaaca atggacatcg tgaataccgc aactgttgag
    26581 taaaaagaaa tccctgcaac agaatgggcg ccgataagat caagaatcgg tttgatcaac
    26641 acgcctccac ccatcccgga aatagcgcca actgtgttcg ccagaacgat caccacaaaa
    26701 taaatcagac caatcatctt gtatttcctt tctcttgttt atatctttta gcgattgtta
    26761 ctcttttctt ttattataca gtaaaagaaa caaacgaggc ttcgaaagct tttctacttt
    26821 ttaaggaaat ttagtgctaa acgaaaagag gttgtgaaaa agctgtcctg catcaagtaa
    26881 taagaacagc caaacaaaaa tagctcctca taattttcgt ttggttgaac ttattaccgc
    26941 agatgcaagc ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt tcagctccga
    27001 gtcacaagga acaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga
    27061 ccgaggagtt ggcaggagaa caccgtttaa ggaagagact gtgaagtcaa cgttttgacc
    27121 tgattattag agaaacaaat ggcaaaatag ttcttcatat ttcgccgttt ttcgagcgaa
    27181 tgccgagggg cagctgattg aacatcgttt atttggaaaa agggattgtg acaagacgtt
    27241 tgtcacaatc ccttttttta tatcttatta attcattctt ttgttctatt cttcttcttc
    27301 cgccatgaat gagaaaccca tatcaaagaa ctataaaggt tatcaaaaca gcgtttatga
    27361 ctaaatgaat tagagttatc tatcactatt tttcaagcga tttaaaccaa atggcataca
    27421 atatggcata caaaaacttc aataagccgt gggggttttt aaatttcttc ttgttcttta
    27481 aaatcaatga gtggagctac ataatttgaa tccacttttt caaacgtcac tttttgcatc
    27541 gacgaatgtt ttctttccca agataacgta aatagatttg actctggact tataaagtct
    27601 acttcacctt ctagtattac tttcttatgc aaatattcct ctaataaata tagacttgct
    27661 aaagcataca acacattttc caaatttgct tccttataat ttgaagaacg accatgtttt
    27721 actctgttat atattttcca ccaatctggt gtatcttcag gccacccatc tccaaaaggt
    27781 tttagtgaag tcaatgttaa agaattcata accctaacac cattatccaa acacattaat
    27841 tcgctagatt taataacagc tttaaaatca gttatgcttt tattgctact gctatcaaat
    27901 ccgcaaagtt ctttgaaaat aatctcacac tccgaaccaa tcgctagtag tagtttttga
    27961 tattcaatag agaatgtttt gaaattattc tcatcgagca tcacaaaatc tgtggtatta
    28021 ataaactcat tttctaaatg tagataatac ttccaataaa aatttgtaaa ctctgctttg
    28081 tccattaaat cacctctttt gaattataac aaaaaaaaca cgtccacaac agacgtgttc
    28141 tttgatttct acacaatcag cttgccaaaa ccatccaatc agtcctagca tgcctgtatt
    28201 aaaattgaac gttcatcatc tatcatcttt cttagtccag catgtttgca accacaccta
    28261 caattttcag tccaacaata gatttcgact agctaagtct tgcaaaattc aggcagctaa
    28321 cccttcattt actaccttaa ttatactaaa tacccacaac gtttacaagt gtaatcattc
    28381 taagaaatct gtttagctat gatcgttgta taatctttaa acgccgttcc ttcattgatt
    28441 gaaataattt tataactctt gtttttccaa gctactttca tatcgtttgt tagttcataa
    28501 tcttgatcgt atcgaataat aaacgtcaac gttccttcaa gaatggtacc tacactagca
    28561 agcacgtctt ttagcatttg tgaacgcaca ttagcccaac attcaaatac agtttcttca
    28621 ctatccacca tctgcccatc ttcatctttt acccgttttg tctttttaaa cttgactctc
    28681 tcagtcaatt catttacatt cttaatgatt ggcataataa gcacctctca tttgttgtac
    28741 taatgcttgt accgttactg gtatccgttc gctgctggct tcttgacggt tcaaatacca
    28801 atgttgagta agtaatgaca ccgccaaatc aaattgctga taattatcca tatctttatc
    28861 cgttgcgcta ctatcaatag cactttttat atattcgcct gctgtatcaa tcaattgttg
    28921 aataaagtca tcatcaatag tgtgatcgat ccgcatactt ttttttatgc tatccaaaat
    28981 taccatttta tctctcctaa tgaaaaaaag gcagtgggat aaaccgccgc ccaatatttt
    29041 tctatacttt tggtgttaca actggcgtaa actcaatata cactgaagca ttttgatcaa
    29101 ttttcttata atcgttgcga acgatcactg caagtccttg tgagtagtaa tcgaattttt
    29161 cccattgagt cgttacttga tttctacgag caacaaagat agattgtgca atatccccca
    29221 taatcatagg gaatgttcca gctttaggat tggcaaacaa tgtatctgca atcaatacta
    29281 ctggcatacc gaataatgat ttaccactag gtgctgtcac atcgggttgt aaaatgtaac
    29341 gtccatctga atctttcaat gtatctaggt ggtaatatcc gctttggttt agaattacca
    29401 ttttatttaa tgctgggtct aatgccacat tgtatagttg tttcaaatcg tccaacgtag
    29461 cagccgtttt cttagtgaat gtttttaata aatccatgat atgcttattg tcagtgtttt
    29521 ctaccaattt cgctaactga tctttgacct cttgtacaat attcactgct gaatcttcca
    29581 caacctcatt tgataaggca atcttaccag cacgagtttc tactttataa tcaactgaag
    29641 taaacatttc agcgtcaata tcaccaattt cagcaagttc agcttttgtt gctaacactg
    29701 cttgttggtt agttgctact ggatatttac cttgtccatt tgatactgtt tttactgttg
    29761 catattgagc taagttataa tttgaacgtt tcaaatcaaa gacttcaccg attacttctt
    29821 cgggaacaac tgccgctgca tttacagtag tgactccatc acgcacttcg ccttgtgaac
    29881 ggatatattc ttcataccca cgcaattctg ttttttcgtt atcgattaaa gttttttcca
    29941 tgttattact ctccttttgt gctttagaag tggattctaa gctattgttt gtttgttttg
    30001 cttggttgtt actcataaac gattcgtaag aacgcttatt tacttggaca tttgatgaat
    30061 cataagcggg aacggtcacg acactaattt cattaagtgc tttgatttta tctattgatc
    30121 gttcaattgt gccatcttct tttttgtcga atgaatctaa tccaagcacg aacccaaagg
    30181 acatagaatc aacaacccct tttgagatat tctcatacac atcatttgaa taggtcgtgt
    30241 cggttagctc tgcttcaaaa tgtaatcctg tatcatcgac attcaatttc aacgtacctg
    30301 ctttgacact agctagtggc tttgaataat cgtgtccata taataagaac acatttgata
    30361 aatccacctc ttttaatgct tcgggtgtaa tcacttctac aaagccgcct aaatcttttg
    30421 acggttgccc gaatttcaac gcatagcctg aaatagtttt gccgtctgtt ggttgttttt
    30481 cattttcttt ctctactttg ggagaatcgg ctgttagctc agcttcttct gtcagccgtt
    30541 tttccttttc ttcttcatta ttcaactggt gtaactcctt cctgttcttt taattgatat
    30601 ttctccaacg tatctaaata cgtaaagttc aaacttgcta gtaaacgatc gccaccatcc
    30661 atggggggta gccccatttt cgaccgcccc tcattaatcg tgagaagtga ccctttgacc
    30721 tgatccaata cattttttac ttttgtttca ggatcagtct ctagtaactg atcggaatta
    30781 aatcggatat tggtttcaag tttcgtatct aattcactca caaacacatt aaagtaatga
    30841 atcagcgtac tttggatata ctggagatta ctttgaacgg tgctagaatg ttcattttca
    30901 acgcctaaac gctcaatcgg tacaccaaac gccttggcaa tttgttttgt gttccaatca
    30961 ttggaattga taagcttcaa aacatcggtg tttatttcta atgttttgta gtccattgtt
    31021 tcatcaagaa tgatcgttct aagagcatta tctccactac ttgaaccgtt ggcttcttca
    31081 aacttttcac gaattgcgtt ctttgctgat ccgtctaaat ctgacttatg aaccttcaaa
    31141 atacctgacc cactgacacc acgagtaaag aaattgtgca atgtgcggtt gccagcttgt
    31201 tgaattttca gttcatcacg taacgcataa agtggtggta atcctgttaa accatcttgg
    31261 gtgaaatatt tgaagtgcaa aatatcgcta gacttcacac gtcttttttt atcaccaatc
    31321 tgataagaca acgtaccatt atctaattga gtaaccgtta cacttgagtt aggtaataag
    31381 tgaatttctt ctaccttttc accgttgcgt ttaatctctg caaagctatt accgtttagc
    31441 aacatattga ccgccaaagc aaactttaaa tgccaaccat ccatttctga attaggtttc
    31501 tcgtttagta acttcaccag ttcattatca gcttgtggca tattgttttt gaccaattga
    31561 atcggacttg aagcaatatc actagcaata attcgaacgg ctgcaaacac atcactattt
    31621 cttaatgcac gaacgcttgt aaagctggtg gtgtaatcat ccgattggat tgataccaca
    31681 tgttgtagga aaggatctcc cgttgtattt tgtctcgctt taaaaaatgc catcttttaa
    31741 cctcccttct gttctgtatt tagcatgacc gctaccccaa taagcactat gcccagcgct
    31801 aacaagccat agtagacatt tgttagtaag gtgatagcaa ccaacacaca caccaatcca
    31861 agaagtaaaa gaacggtatg aatgtaatta gaaagaaaat tcttcacttg tataaaattc
    31921 attgtctgct ttcgctcctt ccgttgtggt gaagtaatcc attgcaaaca cataggcatt
    31981 gattaatgca gctattggat caattttgtt actgttcttt gatttattaa tctgaatgcc
    32041 gttgttatcc gtttttaaaa tagcgttgtt cactgcatgg gtgagaatcg tatttttatt
    32101 gtgaataacg ttgccttcat aaacttgttc tctaaaagtt cgggtcggaa cgtttagagt
    32161 aatcgttcct tgtcttactt ctaacattgg gtagttggct ttttcagctt tactgataag
    32221 cgaattagcg ttatacgggt cataacaaat ccccatacat tccaaatcat tttcttgaat
    32281 caaatcttga ataaactgaa atacttggtc atagtccacg attccacttt ctaattgagt
    32341 aatggaacac tcaccagctt tttctagttc tcgataaggc aaaccgtcgc gcttttcttt
    32401 gtcttgaagc ccatatttgg tggctacaaa gctatgtgaa tcacaataaa gttcgccgtt
    32461 atctagcggt acgatccatg aaacgctagt taaatcatct gttttagata aatccactcc
    32521 gatataaacg ggcttgcctg taatatcttg tggttctact tcggttgcct gccagtcttc
    32581 agcaatcata tagctgtcct cactcgcttg tctccataaa ttgaagtttt tcaccaatac
    32641 agaattaagg ttattttgtt tcaaagcaag gttcacatca tcttgaatgg ttggaatcat
    32701 tacttttttg atttcttcac tttcaaaaat cggattggct ttgatccatt tctcttgatc
    32761 gtgaatttct tcgggatcgt ccaattccca tatcgctata aagtacctgt cggcttgttc
    32821 ttcgcctttt agcacacgat ctaacaataa gtattcctcg tacataggaa cgtttaaatc
    32881 aagcccagcg gtactaataa cacacaataa gccgttcttt tgttgggtca ttcctgactt
    32941 aatgacgttg tacgttttac gagtttttgc ttcgtgccac tcgtcaagaa ttgcggttgt
    33001 tggtgcaaac ccatctagcg tgctggtttc actagccagt gccatagcga acgaattaga
    33061 gggcaagtgc tgaatcttag agttcataat tttcagttgt ggtcttaaaa acttactaga
    33121 cttgaccacg tttctaagtg aattagacag catatcatag cccaatttag cttgtttcaa
    33181 agcgttactt acaaacaata cttgccttgc ttctgctggt tcttgttcca taatcaacgc
    33241 attggcagcc atgccacttg cgatatacgt tttcccattc ttacggctca tactggtaaa
    33301 cgctcgattg aatcgcctgt aattcccttc tttagtacgc cagccataaa gactgcctag
    33361 aatgaatttt tgaaatccta acatcttgat tgctttaccg tccgtagatg gtagcaactc
    33421 aatgaattta atcgctttgg tggcttgttc ttcatcgaac acataaggga agctatctga
    33481 cttagaacgc tccaaatcgc ttatatgacg ctcacacgct tggataattt tcttgggtgc
    33541 aatcagctta cctgatagga ctttctcaat gtaatcattc atgaattcat caactctttg
    33601 aacgggtctt cgggttcatc atttttaacc ttgttcaaag ctagttttga tcgtgattct
    33661 aaggtcatac ctagtgaggt ggcttgtttt ttcaaatcac tcatagcttg tgattgaacc
    33721 cgaaccgctg gattggcttt ctttactccg ttttcgcatg tgataaacgt acctgatttt
    33781 cttatctcgg cttcggctgt tttcattcga gcaaaggcta aacaataggc taccaatgaa
    33841 ccataatctg cttcactcaa tggataatct ttttttaatg ttggtacgat acgattccat
    33901 tcacttctag cgctcgctgc catccagtca gggggcgtga tatttattaa tggttgctgc
    33961 ttgaacaatt cttctctagc gtccacacgg attgcttttt cttcacttga tatattgcct
    34021 tttgtatcgt gtaatatttt agggtgtccc atgttgtcct cctttcagac aaaagaaaaa
    34081 ggaacgtaag caattaagct cacattcctt cgtttttcga tcagaatttt tctttatcta
    34141 atttatctat ataaatgatt tttccgttct gcatttgaat ggtcacttga ccaaaatgag
    34201 gtaactcaat ggtgcgctgt ttccctgttt tatcagtaat aacaattgta tttttcaata
    34261 aatctcctac tttcattata tactttttca cttgtgatta caagtgtaat cgttatttgt
    34321 aattaccctt atgccttttc acaacggtta tttgtaccaa aatgagtagg catcgattgt
    34381 cagtaagttt cacactatgc ccccttaact caaagggtac tcttttctcg tctttgtctt
    34441 tcattggctg tcttggaatt atggtgtgca tggcataaag aacgaaaatt agtttcatct
    34501 aaccgtctat tccaatcatc ttttagttca attttgtgat ctacaacatc agctttcctt
    34561 atcaagcctt cttctaggca atcctcacat actggatgat ttaaccgata caagtatgaa
    34621 gcctttcgcc atgttctact ttgatagaat tgaaaatatt taccttcgct ttgctttctt
    34681 tcttcacgct taattgccgt ttgctttgct ttatgcttcc cacaatactt ctgcctataa
    34741 tcaatcaatg ttttacatcc agcatgatta cattgttttt taactgccat agattaccaa
    34801 ccaaagcgat ctcgttcgtc agaatcatct tgttcccatt ttgatttgta ttcaatataa
    34861 ggcgaaacta tcttcaagca cgttccgtct ttaaaattaa ctttagtgat tttatcttcc
    34921 tcaaaactaa taaagtctac atcttcttga gtgtttgaat actcaatgtg tttctcacca
    34981 tcgaagtatc taatactttc catatgcata attattctac ctccaataaa ttagttatca
    35041 attcatcatc tgttagtagc aaagccaaga acttatcatg gatgtttaaa aacaaattat
    35101 cctcattcga tgtaagctct cgttgtctcg ctcgatcata taggttaagc agacaattaa
    35161 attcatccat tagctcacct gtatcatcat agttgccact atctaccaat gatcccacta
    35221 ctagcgattg acaccacgct actgactcct gcagccaatc aggttcttct tccattatct
    35281 ttaatacttc tggatcaaat gtggaataat cttctgttac caatcccaca ctaaatgcca
    35341 gcgtgttcat tagaaaccga tccatttcat ctgtatattg aaagttcggt gctttgtttg
    35401 ttgaaataat attgagcatt tactttcctc cttgattcgt aacacgtggt aacacctacg
    35461 taacaggtac ccgttaccac tcaatcccaa cggggacaag ggttctagcg ttttggtaac
    35521 aggtaacggg taaaatccag taacattatt ccccagcata ttagctaact atttcaaaag
    35581 ttttaaggaa ataaggtgtt accccgttac caaattgctg tatcccttgg ggcagtaagg
    35641 gtaaactagt aacgggctag gtgttactga cccgttacta cccgttactt tttgtacttt
    35701 ccagctcgat ttttattttc ataggcattt gacttattgt aaaatcctcg tcgtttattt
    35761 ctttttggca gttgcttccg ttcccaacca ggaatactgc taataatcaa ggcaatcttt
    35821 ttagtttctg catctgatcg accattcaat aaatcctttg cttgtttatc aaaaacagct
    35881 tccaaaatat cagcggtgag tacgctatca actaaataaa actcttgttg catagtagct
    35941 ccattataga aatgctgtaa atttttctca ttacatttac tgtcaggata gttctgataa
    36001 taagaacgtc tcgcccacgc tggagcttct tcccatttat acgggatttc catttccaaa
    36061 tatttagtga ttgcttcttt aattggattc tccatcttgg catcctcttg atagtctttt
    36121 gcaatttcta agacttcttt atcttcatcg ggactaaaat agattcgttg tcctttatca
    36181 aaaagcacct ttgcttcagc taacacttga agaaaatatt catcctttgt tttaaacgga
    36241 ttcatctttg gcttattctt acaaggcaaa ggataaaaac ggcggtttcc tgtatcatct
    36301 ttcaaatact gcccatcatt cgacgttcca ataaatacac attgccttgc ccaagctatg
    36361 acattccgac cataaggagg tctaatttta tcgatcttgg cactgatgaa gttttttatc
    36421 tgttcaattt tagttttgtt cattgaagat aattctccaa gttcaataat tacattgcct
    36481 tgaagctgca tataatcatc tttcttttca cctagcgaat ctaatgaatc ggtgaaaaaa
    36541 tcatccgtgt aaagtaatgc tggaacggta ctcttaccaa tcccttgcgg tccatccaaa
    36601 actggaacga tctcaaactt tattcctggt ttatagatac gtgcgactga tccgactatc
    36661 caacgttttg ttacttctcg tgtgtaaagg ttgtcgtcta cccctaaaag gtcaataaac
    36721 agtgtttcca gtctaggctg ttgatcccat ttaacttttt cgatacgttc ttttactggg
    36781 ttgaaaacat tctttctagc cacatttact actgctttgt gtattttgtt gtcatcaaaa
    36841 actacattgt agttttcttc aatatatgcc gttaaggcat cctcaaaact gtcttcccat
    36901 tctccagcgg tacgattgat ccaaggagaa tcttttaata ggtgaatgta tccgctaaat
    36961 tcgttataag caattgttcc agctaaattg ctatcattgg ttaaaatcaa ttctgcattc
    37021 acaacactgt tacttttaat ctgatcgtct ttattgcatc ttaatcgtga catccaggtt
    37081 ttcaattcaa tgatattgtc ttcacgctcg ctggcttcgg gaaagtctaa aatactatct
    37141 aatttatgct tatgagccat gagctttccc cctcaaatct tttttaatca tcgatcgaat
    37201 aatttgattc acttctttat cttttagcgg tggttcattg tacgcatttg ctgtataaat
    37261 caattctgct aaagtttgat tatctacacc gtgccatagt aaccaaccgc ataacttagt
    37321 taaatgatcg tttctactac cttcgccagc gccttgaaca atcatatcta atttttttgc
    37381 tgtccctgtt tttgctacgg gtcggtattg agtctttatt tcttgcggtt tttttagcag
    37441 taggttcact agccaatcgg gagctggtgc aatattattt agagatacgt tttcaatagg
    37501 ttcatatgct ctcccaacac ttcttgaaat aatcatagtt ggcgctattg gaactccttt
    37561 agtgataata tcaatcccgc ttaaactttt acctttatca tcacgcccga agatatcagc
    37621 aaatgcacct gtttcttgtt ggataattac accatcagga attttaaaga agaaatggat
    37681 tcctttacct ggtgtgattt ccgtataagt agaaggaaat ggatcataca ttgaagcaag
    37741 ttttttataa ttctcaacgc cgtttcgtgt ttcagaatgt tgatctaggt caactaccac
    37801 catttgatga tcttcaagca ttaacccaat atttctagtt gcatcttccg tccaccattc
    37861 attaatttga ttagaatcgt tagttgcatc attccaacca ctagagcctt ttattggaat
    37921 tttagttcca ggagataagg gataaattga ccatcccgac ttttgtaatt ccgttgctgc
    37981 ttgataatta tttgccaata aacctctcct cctttttcaa aatactggta taaatcattc
    38041 agacacctcc atgagcttca actgctcaag ttcgtatttc aacccatccc gaacaaattc
    38101 agcacctttt aattgattct gatactttac ccatgtatta aatgttttca cccttgcttc
    38161 ttgcatatca gtaatgttta accatgcggt taaaatctga ttttttagct cgttgatttc
    38221 tcgacaaatt attttgtgtt ctgcatgttt tttgagtaca tacaaaactg ttagtttttc
    38281 attgaatgaa tttccaaagt aggttttccc gatattattt tctagttcag cgatcccttg
    38341 attaatttcc tctaattcaa ggctatcgag ttggtttaat agttttgttt tgtacatcta
    38401 cttatgccct ccagttctta attgatagtc gtactcgtca taagcagtga agagatataa
    38461 cgctaatcct gcaaagatag ctatttttgc aattaatggt aataagccga aaattccgcc
    38521 gattaaaatg cttcctataa ttaagtttcg tttcataatg gttccctccg attttttaat
    38581 atggtataat cggaacaaag aatatcgggt tttatcccaa ttaagttgcc tttatcctgt
    38641 ccgccaagac tgttatggat aaggcttttt ctttgttctc gatcatgtta atgctcctaa
    38701 gttgtctgca attcagcaga acgattataa attttctcac tcactgaaaa ctgcaaccca
    38761 tatttttctt taacagtcat taattctact gtctcatcta aaataggttc acgatcaacc
    38821 aacatttcag gagtcatatc tttctttttt accattttgg aatgaccata aatagttgat
    38881 acagctttat tggctatcac attcgcttta atgtagtcta ctggttttgg ttcagtaata
    38941 gcgtggctta atttagtcat tgcttctttt tggtgttctt tgtctagcat gcggaatact
    39001 tgaaagcctt caagtcctgt ggactggcgt agttctttga tgatgtcgaa aatccagtct
    39061 tgaaatgcct cggcttcttt tttacggctt ttgaatgcca tgcggtaaat attttttttc
    39121 gttgatgatg tttacatctt gtgttctgcc caatgaatcg atgaccttac tagtagtaac
    39181 cccatcttta ggcaaagagt gaatggctct agtaacctgt ttaagaccta acgcttccgc
    39241 aacatctttc gccactgccc accattcatc attaatatcg acaaatctta taatgtgtcc
    39301 gttccaaatt tcagttttca ttcaacgtcc tccagttcaa gccctagcaa gctcgctaac
    39361 aactcaactt gctgatacaa aatttcttga tactcttcaa tagaaagtcg ttctttaaac
    39421 tctgcttgta ctaaatcgtt caagccacgc aatactccgc ttaccgttgg ttcttgtggt
    39481 agcaattttt gatagtccat ttgttcgtac aaacattcaa acgtttcttc tagcgctttg
    39541 ccttccaagt ttgctaattc tgccgctgtg tcttctgtaa gaattgttga taatactttt
    39601 tcgattgctt tattttgtgt cgattccatt attatttcct cctattaatc cctttgcccc
    39661 cacggcttgc gtttattttt ggtattcttc cataaagcga tcacattcat cttgtgaaat
    39721 tcttacgact ccatcaacta taatcaactt taaaccgtta ggaacatatt tctgcatcat
    39781 agtattgtat gaaacgttca tatacttagc agcttgtgcc ttgttcatat attttggaaa
    39841 ctggtacttt ttttctaata atgaaagtaa cgttccttga gtttgctcta cttgttgaac
    39901 aactcgatcg tcaataattt taaaagctgg atcatcaagc gtgagtttgt caatttttaa
    39961 ttccatattt atcccccctc gttagcatac atttctatac aaagatttta aaaaaatgca
    40021 agaggctctt gaatacattt ctatacctct tataaactca atataccatc ataccttttc
    40081 atttgcaaga attttaacat acttttttga acttttatgt ttcacacata tttaaatcgt
    40141 gatatagtag agaaaatata tgaatggtgg tgaaaatgtg gctagaaaaa aataccccga
    40201 agcagtggag acaggacaaa gaattagact ggctagaatt gaaaagggac tctcaatgga
    40261 acaacttgga ggtaaattat ccccaccggc cagtaaaggt gccgttagta attgggaaaa
    40321 tggctataat cttcccaata acaccaggct taaacaactt tcaaaggtat tagatgtatc
    40381 cactacctat ctattaaacg gatcttatac attaagagat atgcatatga tgcccgaaat
    40441 taagcgaaat gtgcttgaaa agcaattgaa aaaaactatt gaaaaaaata actctataat
    40501 tgattctaat ctttctaatg ctatcaataa catcgaagaa gataacctga ctgtctcaca
    40561 gaagatttat ttaacaaacg caataaaata tcttaaaaca ggatctcgaa ttgaatttcc
    40621 tgacgggagt accccaattg cttatctcgc agctcttcta aatacgcttt tacgatatga
    40681 ctcattaatt aatgattcag aaaaaaatac agatgagaaa aaagagtatc aagaatttta
    40741 tgaaaatgat cttctaaaag agttcacagc tcttgttact gagctaaata aacactattc
    40801 aaaataataa taaactgcct cctccaaccg cccccacggc ttgctacact atggagggaa
    40861 aactattatg gcaacattca agcaatatga aactaaaaaa ggtaaatttt ggctctatga
    40921 agcctatctt ggaatgaaca aaatgactgg caaacctgat aaggttcgta gacgaggtta
    40981 caaaacaaaa aaagaagccc agcttgcctt gtcacgcttg caagtggact acgataaaaa
    41041 cggtattaaa aaagcgaaca acgaaacgtt taaagaagtt tatgacttgt ggctagaaac
    41101 atataaaacc actgtccgag aagccacctt catgaaaact gaaatcaagt ttagaaagtg
    41161 gattcttcca aaatatggaa atcttcgtgt gaatgaagtc acggtaaaaa aagcccaaca
    41221 aattgtaaat agctgggctg aaactactga ccaatacaag gtacttcatt caacttctaa
    41281 gcgtattttt agctacgcta tcaatcttgg gattattgag actaatccac tagacaacgt
    41341 attaatgcct aaaagaacca ccactaagca taaagaaaag gtcaaagttt acagtaaaga
    41401 acaactgatc accttgtttg attacctaaa cagcaaacct gaaacttatc gaaacgactt
    41461 tgataaaacg ttactacgct ttctctttta tagcggggtt aggatcagtg aagcccttgc
    41521 attgaattgg tcagacattg attttcaaga aaatacggtc accatcaata aaacactaag
    41581 ccaatccaaa aatggctata aaatcagtga tcctaaaact gattgtagtg ctggggttct
    41641 tccactagat gaaagaacaa tttctatatt aaaaaaatgg cagattaatc agcggaaata
    41701 tatgctgaca cttggtgtaa ctgatccgac tatgattttt tgtggtatct ataaagaact
    41761 aatcacacac cacgctattt atgcaagatt gattactatt tgtgaaaaag ctatggttcc
    41821 atttctcggt gtgcatgtag caagacacac tcatgcttca atgctcttgg acgctggagc
    41881 tactatggta gaagttcaaa ctagattacg gcatacaaaa attagtcaga cgatcgattg
    41941 ttacggtcac ttggcaaaag aaacgaaaga aaagactgtt gaaaaattag tacaacactt
    42001 aaatattgac tagatggcat acaatatggc atacagtaaa aaataaaaaa aggagaaacc
    42061 ctatcatatc aaggtttctc ctagctttat agtctattct tcttcttccg ccatgaatgt
    42121 attgaatact tcttcaatca tgtcccattc tgcgtctgtt tcgatttgtt ctaactcgcc
    42181 ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt aattctacat cttcatcttc
    42241 tgaaacacca gcaggatata aaagaacata gttacgtcca aattcttctt gtccgtcaac
    42301 tgttagtagg atttcatata atgtttcatt tccttggtca tccaccaatg tgatgtgttc
    42361 gtgtccttca tggtcgtggg aatggttatg ttctgtcata agattacctc ttttctttta
    42421 ttattaaaat aattgcttac ctgagccgtc taaataattt tgtaagatca tcacagctgc
    42481 taatttatcg atgacttttt ttcgtttggc tcttgaagta tttgcctgct ctactagcat
    42541 acgttctgcc tgaacagtgg ttaatcgttc atcttgataa tcaactggca aggagaatag
    42601 ttctttgatc ttttctccgt atgacatgga agcttctgcc cttggtccga tggaattgtt
    42661 catattttta ggtaatccaa ctacaaaccg gtctacttca tattgtgcta ctaattcctt
    42721 caaccgatca aagccaaact cttcttctgc ttcattgatc cgaatgatct ctactccttg
    42781 agctgtccag ccaaaggggt cactgatagc aacacctact gtcttagaac cgacatccaa
    42841 gcccattact ctcataaatt cactccatga tttgagaggt agtccttgac aattacttcc
    42901 atgatctcat cgcgttcgtg acggcgaatc aaatttcgag catcacgata acgaggaatg
    42961 taggccgggt ctccagaaag caagtagccg acaatctgat tgattggatt gtagcctttt
    43021 tcttccaaag cccgataaac aatcgctaat gtttcgctca cttctttttt gcgactatcg
    43081 tcaaaaccaa aacgtacggt ttcatctgta aaacccatga ttctacacct ctttcccgca
    43141 aggtaattcc agcttacctt ctacaacctt attttactcg aagaaaggga aaactacaaa
    43201 caaatattcc aaacttccga ataaattaag aaacactatt tgtatgtttt tttccttttt
    43261 tctgtagctt tatcaaaagt actcttttca taaagtatac gctatatttc tttgaatgta
    43321 tgtttttaca caattttttc ataatattca taaaaaattc gaatagctgc tactaatttt
    43381 ttgtttttgt tgcttgtcct gtctcataat tgatcgtgta gccatcttga acattgtaga
    43441 taaagacatt gaattcaatt tttttgtctt caatgctttc tgcttccaat tgcactcccc
    43501 tggctaccag ttcattcccc tcaaaatgag gagtgacacg gtagcgtaca tgatgatttg
    43561 tttctttgat atatgccgca atatcatttt cgtgagcaag catgtatgga gcattcaacg
    43621 aacgtgtccc tgtcatcaag tttttaatgt tgttattttg accagtcagc tggaaaccaa
    43681 ttaaatggct gcgattatat aaccattgcc cgtcacttaa tttcttttgt ttccaacctg
    43741 tcgggtcaat atacaaaggc tctctctttt cagtagggaa caaatctttt ccaagcatag
    43801 cattggcttg tcctactctg ttcaatttat caatatttga atagctttcc catgttcctt
    43861 tggaaaggct aagatcctct tttgtaaatt ccggctgatt cccattgatt tctatttctt
    43921 gatgttttcc atcatattct ggcacatccg ctacttgaga agcttgtggt gtggaatcaa
    43981 aaatagttgt aatctgctga aagatatctg aatctttttg tgtggtggaa acaccgaatg
    44041 cccctgcaat cacagcagcg acgatcccca cgatcgcctt ggttgttttt ttgctcattt
    44101 ctttactcct ttactaacag ctccctaaat cttaacatat gttcccctat aaataaatac
    44161 tagaaagaaa tagaaacagc aaaaaataaa acctgtttta agatggctta aaaatacaaa
    44221 aaagagacgg attcctcgtc tctaatcagt caccactcta tctgtaaggt atttattatt
    44281 attcaaaaat tttcttcata ctcaagacaa tacactgatt tcacaaccgc ttggtaaacg
    44341 gtgcgcaaaa gcttgcatct gcggtaataa gtccatccaa gttaaaaaca taagaagttg
    44401 tttttgcttg gctgttctta ttacttgttg cagagcagct ttttcacaac ctattttaag
    44461 cgggtgacgg gaatcgaacc cgcgacatca gcttgggaag ctgggatttt accactaaac
    44521 tacacccgca atacaaaaac agtatacgcc tcttttattc cttcctcaag tttttttatt
    44581 ctttacgctt ctggtatgtt ttgtagtaaa ataaaactaa tcaatcaatt attggagaag
    44641 atgctatgac gggaaaagaa ttagatctgt tgctggacac ttgtttactc gctgggaaaa
    44701 tcatgatgga aagcaatgcg gagatgtatc gggtagaaga tacgatgagt cgaattgctt
    44761 tagcttctgg aaatttccgt ttggtcagct atgtcactca aaccggtctc tttattggtc
    44821 ttgatcgtac ctcgaccatc cgaatggaac aaattaccaa ccgttcgatt aatttagaaa
    44881 aagtagtaaa agtaaatgat ctttcacgta aatatgttgc ttcagagcta acattagaag
    44941 aactttactg cgaactgcaa gcaatcgacc gagaccgttc tttctttcct gtctggttgc
    45001 aaatcgttag cgcggcagtg atcagtggaa caattatggt gttatttggc ggaaagatga
    45061 ccgatttacc tgtgacttta gtcattggag gattgggata cgcagcttat ctatatagtt
    45121 tgaaattttt tagaatcaaa tttttatcgg aatttttatc ttctttattg attggcatag
    45181 ccgctatcta cagcgtacgt tttggcttag gaagcaatca ggatctaatc attatcggat
    45241 gtgtgatgcc tttagttcct ggagttcaaa ttacgaacgc tatccgtgat ttgctagctg
    45301 gacattatgt ctcaggagtt tctcgcggaa cagaagcgat gatgactgct acgatgatcg
    45361 gttttgcgat tgcttttatt ttccagcttt tttattagga aaggaacaca agtatgggga
    45421 cgatatttat tcagttttct tttagttttt tagcgactgc ggcatttgcc gttattacaa
    45481 atgtacctag gcgttcattg atttgttgtg gtctttctgg tgcttttggc tggatgatct
    45541 actggatctg tgttcaaata ggcggatctg ctgcttttgg ttcattgctt ggttctttag
    45601 gagtagcatt catcagcgat cttttctcta aacgtttgaa aatgcccgta acgattttca
    45661 atattcccgg gatggttcct ctagtccctg gtggtttagc ttatcaagcg gttcgaaatc
    45721 tcgttactga atcctatcga gaagcggctc attatgcggt ccaagcaatt atgattgctg
    45781 gagcaatcgc tttaggtctt gtattatcag aagtctttaa ccataacatc cgtaatttca
    45841 aagtaaagcg tgacgcaatc atgatgaaac gaaaaaaacg gctttttaaa taaacgaagt
    45901 gtaacaaaga aaaggttgtg aaagagacgt tcagccttga gcgatgacaa aatatctcac
    45961 aaacattgcg acaagtaacc gcaggagcaa taagaagtaa ttatgagtat taaaaaaagt
    46021 tttgtaaaaa tagcttctca catttttaca taaaattgac ttaatactga gaaactgact
    46081 tttgaacacc ctctatctgt aaaaaagggg ctgcgacatg atttttgtca cggcccctct
    46141 tctttattca aattttttcc aaaacgttcg ttgataataa acgatccata gagcggaaag
    46201 tattagaact aaaccgatca caggaatatt cactaaccaa aatggataaa acaaagccaa
    46261 cattccgtat aagacgataa gaataatact gatgatcaat gaacccatca tagttgcgac
    46321 taaacccaaa cttcctgaac ctctgttaaa taattggtta atattggtcc agttcagcag
    46381 cagcaaccga taatctctag cgaaataacg tagtgacaat aagtagcagc cgaataaaga
    46441 accaaatagc aagctaggga taaaaaacaa aggcagctga aaagacagtc cgcttaataa
    46501 tgcgatccca ccagttaaaa aactctgcaa tatccatccg aatctgaact tttcttttaa
    46561 atattggttc atagagattg gcaaagaacg gataaacaga aaattttctt gatccaaaga
    46621 aataagattg ctgataaaag aagtctgatt aactgttaag aaagccaatg cgatccctac
    46681 caggaaaaca acaccgatta acttattatc taaatcagtt aattttattt cgcctcctag
    46741 ggcaaaagca agaataaaaa tgattggagt taacaaggaa gaagaaagaa cttgcataat
    46801 caaattaggt tcttttacta attgagcatt gtaactaaat aaaagctgat gcacattttg
    46861 gttcttttta tgggttcttt ttattttgaa ctcactgttt gaatcagtca gttgctctgt
    46921 aagctttggt actatcagct gttttacagc aaggaaaaat cccagagccg tgatagctag
    46981 tattccccaa cccaataaac cttgttgaga aagtggctga ctggcagcat catagaatgg
    47041 caataatatc gaaatcggat gccgatctgt taactcattt tcaatagtcc ccgtttgatg
    47101 attcatccac atgattccag caaatgcgat cagcatcgag ctgccaagca agatactaga
    47161 aactgttttt ctatgttttt taaacactgc tgttcgagcc aaaccaaaaa caatcaaact
    47221 agccagacaa aaaagaataa tcaaaaatag agaaaacaaa aggattgaga taatgatagt
    47281 aagcaaaatc cctagatcag ctcgccatcc ggttaaaata aacatgacta gaagcggaaa
    47341 aacaaaagga atgatggtca aagcaactac caaaatcttt gctgaaaaaa cctgttcttg
    47401 tccaaaaggc agaggcaaaa atacttgcag gtcatttcct tcaaaaaaga tattataaat
    47461 gactgatata ctttgagtta aacctaacat accaaaaagt gccacgtaat aagtaaagaa
    47521 acctggcaat ttaacaaagt tcatagcaaa catagcaaat ccgtaaatca gtaaaaaaat
    47581 cagccctgaa aaaagatttt gagacaaaag atagatagtt aatcgctttc ccttttttcc
    47641 tttctctctt gctttattcg tgagctgtgg attaacatat aaaagattaa ctcgaattaa
    47701 ttctaataat tgtcgcttat acatacggtc catctcctgt cgcttgcctt ccagccattt
    47761 tcaaataaat cttttctagc gtttcatcag gtgagccatt caataattca ctaactgccc
    47821 cctgataaat caattcacct ttcttcaaaa tagccagttt atcgcataac tgttgtgcgg
    47881 tatccaatac atgtgtagaa aaaatgactg tttttccttt tgcggcatga gccttcatca
    47941 tttctttcaa atcaaatgct gcttgaggat caagtccttg caaaggttca tccaataccc
    48001 aaatatctgg atctggcagc aacgtgccga tcaaaatcgt tttttgacgc attccatgtg
    48061 aaaaacttgc gattgtttca ttttgatgac tatacatatc aaaaagcgct gtcaattctg
    48121 ctaaacgctt ttcttttttt tgtgtgtcta attcataggc agctgaaatc aagtcccaat
    48181 attctccagc cgtcaattgt aaaaaaatat ctggggaatc aggcacataa ccgatttttt
    48241 gtttgatact caatcgattt tccgtcaatt tcattccatc tactaaaatc gttcccgatg
    48301 atggttcaat aatactaact aaacttttaa tcgtagttga ttttccggca ccattatgtc
    48361 ctaaaaatcc aaaaatttcc ccttgtctta tcgagagaga caagtctttt aatgcttgtt
    48421 ttttcccgta acttttactt acattgatga attcaatcat ttctgtactc cttttgtttg
    48481 ttattacttt ttattgtacc cttcgttatt ggtggagtaa atgtcaaaaa gagtgcataa
    48541 caaaaaagga tagaaacgac taggctgcta cttgggttgt tctcttctac aggaaaacac
    48601 tcttctaaca ggtaagatat gagtctttgt tttctttaaa atgattcatt ttctatcttt
    48661 tcttgcataa aattctttag tttatcaata cttctctttc ccatctctag ttgttctcca
    48721 tttgcaaatg ttactatttc gtttactctg ctaatagatg taatgttttc agtattaatg
    48781 atataagctt ttaaatcttt acataaatac tcatattcga gtgatttttt atatttttta
    48841 attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt atttttataa
    48901 cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc gctattacca
    48961 taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt ttctttttct
    49021 ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc ttttatcaaa
    49081 taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg atgagacgtt
    49141 agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag ttcgataccg
    49201 ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata gatttcatta
    49261 tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc aaatttccaa
    49321 ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc aaaaaccaaa
    49381 ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg atattttact
    49441 atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg taattctctt
    49501 cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg ctgcttctaa
    49561 aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg acgtccttgc
    49621 gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac gatacacata
    49681 ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg ggatttcacg
    49741 cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc attcatcatt
    49801 tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg aatctgccac
    49861 acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt tcggtgtttg
    49921 gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt aattataaat
    49981 tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct tatccccata
    50041 cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat aaatactggt
    50101 atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt tacttactgg
    50161 gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg gaaacaacgt
    50221 ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat aaggttttga
    50281 caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt ttttgaccca
    50341 caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa tagctaagta
    50401 gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt acatcatcaa
    50461 ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa ataaataatg
    50521 tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag cgtcctgtaa
    50581 aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag accaaaaagg
    50641 cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac gcatctgctt
    50701 ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct aaaatctttg
    50761 ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca gctgcgatta
    50821 atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa tgtgtcaaca
    50881 catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt tgttcttctt
    50941 catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct ttcaatacag
    51001 cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa aagctgccta
    51061 tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg gaaaaaatca
    51121 cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga actggtacgc
    51181 cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa ggaaagaaaa
    51241 atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc catctcttca
    51301 cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct aataatacga
    51361 aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa accgaatttg
    51421 gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct ttgcaaatat
    51481 acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc ttatttttta
    51541 ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct catacatatc
    51601 acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac tgaagttcaa
    51661 gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa ttgcagtgtt
    51721 tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat tccggcactt
    51781 catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct gactgcgcca
    51841 gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt catcgacttt
    51901 aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct ttcttatgaa
    51961 gaaatcttct gaacaaaata atcgttgtcc aaacagaaaa aagcgattga acaagtgaaa
    52021 gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt tcggaatgcg
    52081 catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc aaatcaacat
    52141 tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc aagattcctt
    52201 tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg ggtatcgtta
    52261 aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc attggataaa
    52321 cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa gcacaactga
    52381 tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc aagttcccta
    52441 caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca aaaaagtgca
    52501 taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa taagaaaaat
    52561 ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt gaagacactt
    52621 ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc agtcttttag
    52681 ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt gtctaattac
    52741 atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca ttgctcagat
    52801 attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc gcagtataat
    52861 ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga attcacaaag
    52921 tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc attttggtgc
    52981 agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg tcgctttagt
    53041 tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa ttgaagcacc
    53101 tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg ctcaggacac
    53161 aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg aacaagttag
    53221 acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta cgccagacac
    53281 acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt tttcagatcg
    53341 aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta aattcgattc
    53401 ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac gtttagcttt
    53461 agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa cgaaccattt
    53521 agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg gtgcattatt
    53581 gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct atgaattaag
    53641 cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt tgaaagcaga
    53701 acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc ataaattaga
    53761 agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc aaagccgcag
    53821 aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa aacaagcaaa
    53881 ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg aagatgccgc
    53941 tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc gtcgtcaaga
    54001 agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga aatcaatcat
    54061 tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca ccatcggcta
    54121 ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag aattgtggga
    54181 tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt tcttattttc
    54241 tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag cacgggttgc
    54301 tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac caacaaacca
    54361 tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg acggcacact
    54421 gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag tcgtcgaact
    54481 ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag agaaaaagca
    54541 ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg ctcctgctcc
    54601 cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc gtagccttca
    54661 acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga tcgatcagtt
    54721 ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca atgagctgaa
    54781 ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat gggaagaact
    54841 aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa accattcatg
    54901 ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta cctttttcac
    54961 caataagaag accaattact agtcattctc actaaagctt ctaaataata gtaatcaccc
    55021 caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga ctgtgttaaa
    55081 aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat aattttccaa
    55141 acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc tattaattct
    55201 aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct gctagaatca
    55261 tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat aaaataattg
    55321 gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata acttaatgca
    55381 aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact ataaccttga
    55441 tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt atgataagtt
    55501 gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg agcagctttt
    55561 ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag attcatgcaa
    55621 caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca agcttgtata
    55681 actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat tgaagcttcc
    55741 ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga atacaaaaaa
    55801 ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa atctatatct
    55861 gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa caaccaatta
    55921 attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata acgtcctttt
    55981 ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat taacctctcc
    56041 gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa ttctttataa
    56101 cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt atcaagcatt
    56161 tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt tggatagtta
    56221 gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct aaactataat
    56281 cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt ccaaatgtac
    56341 cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg ggcaaaccca
    56401 taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata cctgaaaagc
    56461 cagcttcact caccctatag ttttccttgt cttcttctat gattattcct tctttctcag
    56521 gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct aattctactt
    56581 tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt tttgcctgta
    56641 aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct cgtacattat
    56701 ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg gtcgtgatat
    56761 atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct ttcaattgat
    56821 tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa tatttttcat
    56881 tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat gtatgtccat
    56941 tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca cgaataggca
    57001 taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac aataaaaaaa
    57061 tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat gtcaattgag
    57121 ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt tgcatccacc
    57181 atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc aaaagatcgg
    57241 cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg cttcgaccaa
    57301 aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa aaatcaatag
    57361 ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta taaatcaaac
    57421 cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa ctgtctcgat
    57481 accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc agtgctgatt
    57541 ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta aaaaattgcc
    57601 aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata atctggcttt
    57661 tttctcctgt atctaatttt ttccataata attgttcatg atgatataac gctaataaca
    57721 ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa taatcgggat
    57781 gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat cgttcatcta
    57841 attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc attcctgcaa
    57901 ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca cttgctcgat
    57961 aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc tgtctggtcc
    58021 ttaatagtgg tactggatgt ttcattgtat tttcccctct ttttactacc aaatagctaa
    58081 ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat agatcagtgg
    58141 cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga tcccatcttc
    58201 atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga tagcagcttc
    58261 atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa gaccgcatgc
    58321 agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat caatgagagg
    58381 aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt ctgctatagc
    58441 tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata aagcccatgc
    58501 atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta accgaatata
    58561 tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt tcctcactag
    58621 atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg gatcaccagt
    58681 ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa tcatccaacc
    58741 aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc gtccagcaag
    58801 taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact gttgataatg
    58861 tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg aaaaatggta
    58921 cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact tttctcgttt
    58981 tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc accagtcgat
    59041 tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg gaattttatt
    59101 ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa tttttcttga
    59161 ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt tatgaatgtg
    59221 gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac ttatttgcag
    59281 ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag accattggga
    59341 tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca cctgtactag
    59401 aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct gaaagcatga
    59461 ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca ctagtaataa
    59521 tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca ttggcaccat
    59581 cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt cgcatcataa
    59641 acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta ttcgataatc
    59701 ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc accatcctag
    59761 atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt gctaatgcat
    59821 aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca ataacaatcg
    59881 aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag ttttttaata
    59941 gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta ctttgcgttg
    60001 aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac acaataatga
    60061 cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc tccgaattta
    60121 aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat agcagaggct
    60181 tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac gaccaaagct
    60241 gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa attacccaag
    60301 ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat ggggatcgtt
    60361 aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc gtatatctct
    60421 ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac actaagccaa
    60481 atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca ctgtacaccg
    60541 ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa aaaagtcatg
    60601 aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac agttctaaaa
    60661 aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat agctaatagc
    60721 atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga tttaataaac
    60781 aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa tgagtagcca
    60841 attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt cacaataaag
    60901 aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt atctgttgct
    60961 tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat cgttaagtcc
    61021 tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa aattttctgc
    61081 ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg cagcggtagt
    61141 caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg tattttcatt
    61201 gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact ttttctcagc
    61261 tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca gtgcgttggc
    61321 taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt gtggattttt
    61381 ggcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc ctggattttc
    61441 ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag gaaaattcaa
    61501 agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt gaaatgcttt
    61561 ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt ctttgattaa
    61621 ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt catcaagcaa
    61681 agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag attctggcaa
    61741 ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga tatattcttc
    61801 ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg gttgataata
    61861 acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac tcgtttgttg
    61921 acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat aaaatccata
    61981 ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg ctgaagtgtc
    62041 tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg tgatcatttg
    62101 tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg ctccagtttt
    62161 gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt ttgcgacata
    62221 tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg ttacttcttc
    62281 gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca aagctatcac
    62341 caatttgtat cttttttcat gtctatccct tctttctttt caagttctta tataaaacgc
    62401 tttcacttat gtatattaac ggaattagta acacaaatat ttatataaaa aaatatttta
    62461 cttgtcgttt tggcttttca tttttgttat aatagtcatt aattataaat agaaagcttt
    62521 ttatataaaa agagaaagga aagtggttct tttgttaaca aacaaccaat cctccaatct
    62581 tgtcaaaaaa acactaacct attccataga tacccatctt catttatctg ctgctcccta
    62641 ttttcgacta ctaataaatg aaagcgctaa tttatatgct gaatttgcac ataaatctat
    62701 agaactagat aatccttccc ttttattcat tgataaacag caagaagtcc aaatcttttc
    62761 tactcaaaag gaaacacaaa cttttatcat tatccagttt tattttgatg agcaattatt
    62821 agcacatatt acgggacaat ctttttctat agatcaagaa ataacatcta tcattacttt
    62881 tctgaaaaat gaacagttca aaattgatta tctaaaacat agtaagccag ctatttccta
    62941 tgatcaggaa atcaattctt ttttcatact atgccaacag atgagcattc atttgctttc
    63001 cttattagtt acttattgtc tgcaaaaaat tcaattgtct tccttaccaa ggcattatca
    63061 acaggaactt gctttattga caataccttc aaatgagaaa gtcattccta aaaaaaagca
    63121 tattctttat tcagatgaat tagttgatcc aatcatacga tatttagaag aacatctttc
    63181 agaaaatttt ttgcttgaac aattagccca aatttttttt atcagtccag ccagcttaaa
    63241 aaaacatttt agaaaagtga atggaaaaag tgttattagc tattttaaag aactaaaaat
    63301 ggaaaaagca aaacgtctcg tgctagaaaa tgaattaacc tatacagaaa ttgcagatat
    63361 attaggattt cattcgatcc atcatttttc ttcagctttt aaaaaatata cagggttatc
    63421 cccttcaaag tattatctat ccttaaaata gaaaaaaatt tttaggtttt atccacaaat
    63481 tatttctcta tattttctgt ctattcaaaa taaacaataa ttttccggca caattatttg
    63541 ctgtttttta ccataacata gtacgatcaa cgtagaaaag gagatgtttt tatgaaagtt
    63601 gttgtcattg gcggtgtagc tggcggtcct tcatttgcca ctcgtttccg tcgattgaat
    63661 gaagcgcacg aaatcattat ctatgaacgc ggagaaaata tttcttatgc aagttgtgct
    63721 ttgccttatt atttaggagg tgtgatcact gaccgtgact cgctgatcga acgtacacca
    63781 gaaatattga aaacaaaaaa caacatcgat gtctttacta aacacgaagt aacagcaatc
    63841 gatccttcta ctaagcgatt aacagttaaa gacctatcca caaatgaaga aataaaaaca
    63901 gattacgata agttgatcat ctcttctggt gctagaccag attatccgga tattcccgga
    63961 gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga cggatgcgga tcgaatcaaa
    64021 tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc ttggtgcagg tgttatgggt
    64081 ctggaattag ctgagaattt caagcatcgc ggcttagacg tgactttaat cgatcaattg
    64141 ccacaagtcg ctttccctta tgatccagaa attgctaatt tagtttatga caaattgctg
    64201 aaagaaggat tagccgttca tttagaaaca agagttactg agatccgtga taaaggtcga
    64261 gaaatagtat tatcagatgg ctccgtcctt tctgctgata tgctaatttt tgctgttggt
    64321 gtttctccga agaatgaagt ggtgaaagca accggcatac aattatcaga tacaggtcag
    64381 atcattgttg atgaccagtt acaaaccaat ctgccggaca tctatgcgat tggcgatatt
    64441 atcgaaacga ctagtgtagt gactggtcag ccgatccaaa gtatgctttc cagtgcggcc
    64501 aatcgtcaag gacatatgct ggcagatatt ttaaatggta cgcctatgcg ctatcgcgga
    64561 tatattggtg caggtgtcgc aaaaatcttt gatcatacag caagttatgc gggaatgaca
    64621 gaacatgaat taaaagcatc aggcataaca aattataaaa ctgtttttat cactcccttt
    64681 gaccatgcct atttctatcc agaagctaca agattaaatc taaagctgat ttttgatgca
    64741 gatagcggtc gtattttagg tggacaagca tttggagaaa aaggtgtcga taaacggatg
    64801 ggagaacttt ctgtagcaat caccggaaac ttgacagtct ttgatttgcc tgatttggag
    64861 ttgccttact ctccaccgta ttctactacc cgtgacccgt tgaatatagc tggttatgtc
    64921 gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag caagtgatat acccgaaaac
    64981 gatttgaaag aagcgttctt tttagatata cgtgaaccta ataaagcacc ttctggcagt
    65041 atctcggcaa caaaaaatat tccaatgaat gagttacgag atcgaatcaa tgaaatccca
    65101 aaagataaaa aagtttacat tactttcaga agaggattga atacttatac ttctgcccga
    65161 atcttggcag gtttgggtat caaagcgatt ttgattgaag aataaaaaaa cgaccgcggt
    65221 gggtagccgc ggtctaggag ttatttttac ttgaggagta aaaatgaaaa agtatatagg
    65281 gttgttggtt ggtatgtata tagaatagaa gataaatgtg aagaaattgt gcattatttc
    65341 ttcacattta ttaggttata gtttaagaaa gtttaaacat tgcgcaattt aattatttta
    65401 gctgtttctt ttcataaaaa aacatgttgt gactctgaga caaatcatag tccatagtga
    65461 tgtctctaga gtaactacgc ctaatattac gaggcatcat attaatctat cagcactagt
    65521 acctaaacta gcatcccttt cgaatatttt ttcttatata ataaagtttg ctttcactcc
    65581 tctaatctca ccaaatcgac tggcttgcgt tgtgtgatac taggaactga ttggtttaga
    65641 acgacttttc tattgtaatc atatttttcg ccatcctcgt tgtcttctaa tccttcaatg
    65701 aatgatccct aatgtgtgta ttaaataatt taattttttt gttcctattt atttaa
//
LOCUS       DAFEHS010000007        61060 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFEHS010000007
VERSION     DAFEHS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.007"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(15..701)
                     /gene="gpmA"
                     /locus_tag="LP327_000476"
     CDS             complement(15..701)
                     /gene="gpmA"
                     /locus_tag="LP327_000476"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL1724621.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(873..1454)
                     /locus_tag="LP327_000477"
     CDS             complement(873..1454)
                     /locus_tag="LP327_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL1724622.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1587..2270)
                     /gene="rpiA"
                     /locus_tag="LP327_000478"
     CDS             complement(1587..2270)
                     /gene="rpiA"
                     /locus_tag="LP327_000478"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBL1724623.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2589..3002
                     /gene="rpsL"
                     /locus_tag="LP327_000479"
     CDS             2589..3002
                     /gene="rpsL"
                     /locus_tag="LP327_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBL1724624.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3086..3556
                     /gene="rpsG"
                     /locus_tag="LP327_000480"
     CDS             3086..3556
                     /gene="rpsG"
                     /locus_tag="LP327_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBL1724625.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3650..5737
                     /gene="fusA"
                     /locus_tag="LP327_000481"
     CDS             3650..5737
                     /gene="fusA"
                     /locus_tag="LP327_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL1724626.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5909..7096
                     /gene="tuf"
                     /locus_tag="LP327_000482"
     CDS             5909..7096
                     /gene="tuf"
                     /locus_tag="LP327_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL1724627.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7503..7811
                     /gene="rpsJ"
                     /locus_tag="LP327_000483"
     CDS             7503..7811
                     /gene="rpsJ"
                     /locus_tag="LP327_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBL1724628.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7842..8471
                     /gene="rplC"
                     /locus_tag="LP327_000484"
     CDS             7842..8471
                     /gene="rplC"
                     /locus_tag="LP327_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBL1724629.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8499..9122
                     /gene="rplD"
                     /locus_tag="LP327_000485"
     CDS             8499..9122
                     /gene="rplD"
                     /locus_tag="LP327_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBL1724630.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9122..9412
                     /gene="rplW"
                     /locus_tag="LP327_000486"
     CDS             9122..9412
                     /gene="rplW"
                     /locus_tag="LP327_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBL1724631.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9451..10284
                     /gene="rplB"
                     /locus_tag="LP327_000487"
     CDS             9451..10284
                     /gene="rplB"
                     /locus_tag="LP327_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBL1724632.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10327..10605
                     /gene="rpsS"
                     /locus_tag="LP327_000488"
     CDS             10327..10605
                     /gene="rpsS"
                     /locus_tag="LP327_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBL1724633.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10628..10975
                     /gene="rplV"
                     /locus_tag="LP327_000489"
     CDS             10628..10975
                     /gene="rplV"
                     /locus_tag="LP327_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBL1724634.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10989..11645
                     /gene="rpsC"
                     /locus_tag="LP327_000490"
     CDS             10989..11645
                     /gene="rpsC"
                     /locus_tag="LP327_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBL1724635.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11648..12082
                     /gene="rplP"
                     /locus_tag="LP327_000491"
     CDS             11648..12082
                     /gene="rplP"
                     /locus_tag="LP327_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBL1724636.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12072..12260
                     /gene="rpmC"
                     /locus_tag="LP327_000492"
     CDS             12072..12260
                     /gene="rpmC"
                     /locus_tag="LP327_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBL1724637.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12285..12551
                     /gene="rpsQ"
                     /locus_tag="LP327_000493"
     CDS             12285..12551
                     /gene="rpsQ"
                     /locus_tag="LP327_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBL1724638.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12609..12977
                     /gene="rplN"
                     /locus_tag="LP327_000494"
     CDS             12609..12977
                     /gene="rplN"
                     /locus_tag="LP327_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBL1724639.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13014..13322
                     /gene="rplX"
                     /locus_tag="LP327_000495"
     CDS             13014..13322
                     /gene="rplX"
                     /locus_tag="LP327_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBL1724640.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13350..13889
                     /gene="rplE"
                     /locus_tag="LP327_000496"
     CDS             13350..13889
                     /gene="rplE"
                     /locus_tag="LP327_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBL1724641.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13908..14093
                     /locus_tag="LP327_000497"
     CDS             13908..14093
                     /locus_tag="LP327_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBL1724642.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14129..14527
                     /gene="rpsH"
                     /locus_tag="LP327_000498"
     CDS             14129..14527
                     /gene="rpsH"
                     /locus_tag="LP327_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBL1724643.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14559..15095
                     /gene="rplF"
                     /locus_tag="LP327_000499"
     CDS             14559..15095
                     /gene="rplF"
                     /locus_tag="LP327_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBL1724644.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15256..15612
                     /gene="rplR"
                     /locus_tag="LP327_000500"
     CDS             15256..15612
                     /gene="rplR"
                     /locus_tag="LP327_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBL1724645.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15633..16133
                     /gene="rpsE"
                     /locus_tag="LP327_000501"
     CDS             15633..16133
                     /gene="rpsE"
                     /locus_tag="LP327_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBL1724646.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16148..16327
                     /gene="rpmD"
                     /locus_tag="LP327_000502"
     CDS             16148..16327
                     /gene="rpmD"
                     /locus_tag="LP327_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBL1724647.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16371..16811
                     /gene="rplO"
                     /locus_tag="LP327_000503"
     CDS             16371..16811
                     /gene="rplO"
                     /locus_tag="LP327_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBL1724648.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16811..18106
                     /gene="secY"
                     /locus_tag="LP327_000504"
     CDS             16811..18106
                     /gene="secY"
                     /locus_tag="LP327_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBL1724649.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18168..18815
                     /locus_tag="LP327_000505"
     CDS             18168..18815
                     /locus_tag="LP327_000505"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBL1724650.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19006..19224
                     /gene="infA"
                     /locus_tag="LP327_000506"
     CDS             19006..19224
                     /gene="infA"
                     /locus_tag="LP327_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBL1724651.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19257..19373
                     /gene="rpmJ"
                     /locus_tag="LP327_000507"
     CDS             19257..19373
                     /gene="rpmJ"
                     /locus_tag="LP327_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBL1724652.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19391..19756
                     /gene="rpsM"
                     /locus_tag="LP327_000508"
     CDS             19391..19756
                     /gene="rpsM"
                     /locus_tag="LP327_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBL1724653.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19784..20173
                     /gene="rpsK"
                     /locus_tag="LP327_000509"
     CDS             19784..20173
                     /gene="rpsK"
                     /locus_tag="LP327_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBL1724654.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20253..21191
                     /locus_tag="LP327_000510"
     CDS             20253..21191
                     /locus_tag="LP327_000510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBL1724655.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21219..21599
                     /gene="rplQ"
                     /locus_tag="LP327_000511"
     CDS             21219..21599
                     /gene="rplQ"
                     /locus_tag="LP327_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBL1724656.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21871..22710
                     /locus_tag="LP327_000512"
     CDS             21871..22710
                     /locus_tag="LP327_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL1724657.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22686..23555
                     /locus_tag="LP327_000513"
     CDS             22686..23555
                     /locus_tag="LP327_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL1724658.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23545..24342
                     /locus_tag="LP327_000514"
     CDS             23545..24342
                     /locus_tag="LP327_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL1724659.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24545..25291
                     /gene="truA"
                     /locus_tag="LP327_000515"
     CDS             24545..25291
                     /gene="truA"
                     /locus_tag="LP327_000515"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL1724660.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25624..28293)
                     /locus_tag="LP327_000516"
     CDS             complement(25624..28293)
                     /locus_tag="LP327_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL1724661.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28531..28719)
                     /locus_tag="LP327_000517"
     CDS             complement(28531..28719)
                     /locus_tag="LP327_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBL1724662.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            28989..29351
                     /locus_tag="LP327_000518"
     CDS             28989..29351
                     /locus_tag="LP327_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL1724663.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29403..31085
                     /locus_tag="LP327_000519"
     CDS             29403..31085
                     /locus_tag="LP327_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBL1724664.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31202..33238
                     /gene="recG"
                     /locus_tag="LP327_000520"
     CDS             31202..33238
                     /gene="recG"
                     /locus_tag="LP327_000520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBL1724665.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33287..34288
                     /gene="plsX"
                     /locus_tag="LP327_000521"
     CDS             33287..34288
                     /gene="plsX"
                     /locus_tag="LP327_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBL1724666.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34410..34652
                     /gene="acpP"
                     /locus_tag="LP327_000522"
     CDS             34410..34652
                     /gene="acpP"
                     /locus_tag="LP327_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL1724667.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34920..35924
                     /locus_tag="LP327_000523"
     CDS             34920..35924
                     /locus_tag="LP327_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1724668.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35925..36869
                     /locus_tag="LP327_000524"
     CDS             35925..36869
                     /locus_tag="LP327_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1724669.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36869..37831
                     /locus_tag="LP327_000525"
     CDS             36869..37831
                     /locus_tag="LP327_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1724670.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37858..38772
                     /locus_tag="LP327_000526"
     CDS             37858..38772
                     /locus_tag="LP327_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1724671.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38798..40579
                     /locus_tag="LP327_000527"
     CDS             38798..40579
                     /locus_tag="LP327_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL1724672.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40927..41613
                     /locus_tag="LP327_000528"
     CDS             40927..41613
                     /locus_tag="LP327_000528"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBL1724673.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41633..45214
                     /gene="smc"
                     /locus_tag="LP327_000529"
     CDS             41633..45214
                     /gene="smc"
                     /locus_tag="LP327_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBL1724674.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45223..46032
                     /locus_tag="LP327_000530"
     CDS             45223..46032
                     /locus_tag="LP327_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL1724675.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46043..47041
                     /gene="ftsY"
                     /locus_tag="LP327_000531"
     CDS             46043..47041
                     /gene="ftsY"
                     /locus_tag="LP327_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBL1724676.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47228..49495
                     /gene="gshAB"
                     /locus_tag="LP327_000532"
     CDS             47228..49495
                     /gene="gshAB"
                     /locus_tag="LP327_000532"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBL1724677.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49584..50036)
                     /locus_tag="LP327_000533"
     CDS             complement(49584..50036)
                     /locus_tag="LP327_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBL1724678.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50186..51136
                     /locus_tag="LP327_000534"
     CDS             50186..51136
                     /locus_tag="LP327_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL1724679.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51129..52088
                     /locus_tag="LP327_000535"
     CDS             51129..52088
                     /locus_tag="LP327_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL1724680.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52085..52840
                     /locus_tag="LP327_000536"
     CDS             52085..52840
                     /locus_tag="LP327_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1724681.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52863..53816
                     /locus_tag="LP327_000537"
     CDS             52863..53816
                     /locus_tag="LP327_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL1724682.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(54460..54756)
                     /locus_tag="LP327_000538"
     CDS             complement(54460..54756)
                     /locus_tag="LP327_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBL1724683.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            55111..55467
                     /locus_tag="LP327_000539"
     CDS             55111..55467
                     /locus_tag="LP327_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL1724684.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            55477..56376
                     /locus_tag="LP327_000540"
     CDS             55477..56376
                     /locus_tag="LP327_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBL1724685.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            56893..57624
                     /locus_tag="LP327_000541"
     CDS             56893..57624
                     /locus_tag="LP327_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL1724686.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            57769..59796
                     /locus_tag="LP327_000542"
     CDS             57769..59796
                     /locus_tag="LP327_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL1724687.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            59904..61001
                     /locus_tag="LP327_000543"
     CDS             59904..61001
                     /locus_tag="LP327_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL1724688.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT        20127 a        10958 c        13044 g        16931 t
ORIGIN      
        1 ttttatagtg ttttttatag gtagtatttt ttagcaactg ttaagtcatc atttaattca
       61 taaacaagtg gttgacctgt tggaatttca agatccatga tgtcttcgtc agagatacct
      121 tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg cagctactaa aacagttttg
      181 ttgtctagta atgctggagc aatttcatct tgccagaatg gcaaagcgcg ttctaatgtc
      241 actttcaagt tttctccacc aggaatatcg cgtttgtcta acattgcgta acgacggtca
      301 tttgctgcag aaccttcgtc agtttcttcc attaatggag gaagagtatc gtaagaacga
      361 cgccagatgt gcacttgttc gtcgccgtat ttttcagctg tttctttttt gttcaaacct
      421 tgtaatttac catagtgacg ttcgtttaag cgccatgatt tgatttgtgg tacccaaagt
      481 tgatctgaat attctaagat caagttacat gttttgattg cacgagttaa aacagaagtg
      541 taagcaacat caaattcgat tccagcttct ttgattttgc gtccgccttc ttttgcttct
      601 tcgattcctt ctggtgctag atttacatca gcccaaccag taaattggtt caatgcattc
      661 cattcactaa gaccgtgacg agaaaaaact aattttggca tgtttagctc tccttttaag
      721 ataggtttat atttctgaaa ttttcagttc tgttttattg tacaattttt tggaagagat
      781 ttccatcgaa aaattagata agattctatg agaaaacaag tagagaatat aaaaaaacga
      841 acagtgccca cgcaactgtt cgtttttagg tattagatgc ataattgctc gatttctttt
      901 tcaggtaaat tgatatattt ataacatgtt ccaactgaaa caccgcattt tgaagaaata
      961 aattggatcg tttctttttt ttcataatag agacgtcgga tttttttgat cgtttttgca
     1021 tcgatttttg gtcttccgcc gatttttcct tttttacgag cttcatctaa tccaactaat
     1081 gtacgttctt tgatcaatgc gcactccatg tttgctaggt tttccattaa ttggaaatag
     1141 atttttccca tttcgcgcgt atcgattcct tcatcaagac taatcaagtg cagcccgcga
     1201 tctttgaata attgcgtgag ttctgttaat tgccgcgtag atttgcctaa tcgatgaagt
     1261 tggcaaatga ctagtgcatc gccttgttgc atgtcagcga tcactgactc caaatgaaca
     1321 gcagtagaat cattttgttc cacttcatat gtctcatgga aaatttcttg acaaccaaag
     1381 tcagataatt gatcaacctg agttgttaaa tcaacgtcat ttatggttgt gcgtgcataa
     1441 ccaataattt tcatggtgat tcctttctag cagtgatttt ctaactggat ataacagtaa
     1501 aaatgtagca taaagtacgt caaaaagata gttttttcgc aaattttccg ctgtcttttt
     1561 ttattggatg atcaagcagt ttttctttat ttacgacgga ttgcgtcaag aatctcaacg
     1621 cctttttctg agccgacaac tactttagaa accatgttta ggaaaagccc atgttctact
     1681 accccaacaa gttcatctaa ataggtagct aagctttctg gctgttcgat gacttctaga
     1741 tgcagatcaa tgatgtaatg accaccatct gtggttaaga cttctccgtt ttcgtctgta
     1801 cgcaagacgg gttggaatct tttttcttca aaaatatgaa gtagctgttg ggagccataa
     1861 gggatcactt caactgggag tgggaacttc ccaagtttat cgactaattt agaagaatca
     1921 acgatccaaa tcgtttcttt tgagtacgta gcgactattt tctcgaaaag caaagcagcg
     1981 ccgccgcctt tgatcccttg aaaatcagca ctgatttcat cagcaccgtc tattgtcaaa
     2041 tcgactacgg ggacttcatc aatactcttt aacggtattc ctaatttttc cgcttgttct
     2101 tttgtcgcat ttgaagtagt aactcctgtg atacgtaaac cttcttcacg catacggcga
     2161 ccaatctctt ctaccatata ataggcggtt gaaccggtcc cgagacctac gatcatcccg
     2221 tcctttacga atttcgcggc ttcaatgcca gctaattgtt ttaaattcat ctttgcgtcc
     2281 accttttttt agtttgagca tcgtgctttc ctcactattt tagcgtgatt tacggtaaaa
     2341 agataccatt cttttttatt ccaataaagc tttttcaaaa aaaattgcat ctcttgttga
     2401 catgtttttt cattcgtgat attctaacaa aggtgcttta tctacaggtt tctgtgaaga
     2461 aacgtgctga ctgtttatca aagcatattt gataaggatg cagacattgc atttattttt
     2521 tatcacaaaa aaagaaccac ctggatgtgt ggaactagac gcaaatcgtg ggaaggagga
     2581 aacaaggaat gcctacaatt aaccaattag tacgtaaacc tcgtaaatca aaggtggaaa
     2641 aatctaattc acctgctttg aacaaaggat ataacagctt taagaaaact caaactaacg
     2701 taaactcacc tcaaaaacgt ggggtagcaa ctcgtgtggg tacaatgaca cctaaaaaac
     2761 cgaactcagc tttacgtaaa tatgcacgtg ttcgtttgtc aaacttaatt gaagttactg
     2821 cttatatccc agggattggt cataacttac aagaacacag tgttgtatta ttacgcggtg
     2881 gtcgtgtaaa agacttacca ggggtacgtt accatatcgt tcgcggtgct ttagatactg
     2941 ctggtgttaa cgaccgtaaa caaagccgtt ctaaatacgg aactaaaaaa cctaaagctt
     3001 aattttaatt attaaacaaa tgaaacttgc ttctactata tagaagtaat tactatgaac
     3061 aatatttcgg aaggaggagt tacggatgcc acgtaaaggt cctgttgcaa aacgtgatgt
     3121 tttaccagat cctatttata actcaaaatt agtaactcgc ttgatcaacc gtgtaatggt
     3181 tgacggaaaa cgcgggattg cagctaacat tatctataat gcctttgact tgatcaaaga
     3241 atctacaggt aacgatccat tagaagtgtt tgaacaagca atgaaaaacg ttcaacctgt
     3301 tcttgaagtt aaagcacgtc gtgttggggg ttcaaactac caagtaccag ttgaagttcg
     3361 ccctgaacgc cgcgttactc taggtcttcg ttgggtagta aactacgctc gcttgcgcgg
     3421 tgaacacaca atggaagaac gcttagcaaa agaaatcatg gatgctgcaa acaacacagg
     3481 tgcttctgtt aaaaaacgtg aagacactca taaaatggct gatgccaacc gtgcgtttgc
     3541 tcactatcgc tggtaagatt ctctctgttt ggtcggcttt tataagtcga cctcagatag
     3601 tgtccttatt tttagcaact agatagtaaa gagaggagaa acaaggagaa tgaccagaga
     3661 attttcttta caaaacactc gtaatatcgg tatcatggcc cacatcgatg ctggtaaaac
     3721 gactgctact gaacgtatct tgtactatac tggtaaaatc cataaaattg gtgaaactca
     3781 tgaaggagct tcacaaatgg actggatgga acaagaacaa gaacgtggta ttacgatcac
     3841 ttctgctgct actacagcac aatggaaggg tcaccgaatc aacatcatcg acacaccagg
     3901 acacgtagac ttcacagttg aagttgaacg ttcattgcgt gtacttgatg gtgctgtaac
     3961 tgttcttgat gcacaatcag gtgttgaacc tcaaactgaa acagtttggc gtcaagcaac
     4021 aacttatggt gtacctcgta tcgtatttgc aaacaaaatg gacaagatcg gtgctgactt
     4081 cttatattca gtatcaactt tacatgaccg tttgcaagca aacgctcacc caatccaatt
     4141 accaattggt gcggaagacg acttcacagg aattatcgac cttgtaacaa tgaaggctga
     4201 aatgtataca aacgatcttg gaacagagat cgaagaaaca gaaattcctg aagaataccg
     4261 cgaattagct gaagaatggc gcgaaaaatt ggttgaagct gtagctgaaa ctgatgaaga
     4321 acttactttg aaatacttag aaggtgaaga aatcactgaa gctgagttaa aagaaggtat
     4381 ccgccgcgca acagttaacg tagaattcta cccagtacta tgtggttcag catttaagaa
     4441 caaaggggtt caattactat tggacgctgt tcttgattac ttgccatcac cgttagatat
     4501 cccagctatc aaagggattg accctaaaac tgatgaagaa gtagaacgtc ctgcagacga
     4561 cagcgctcct ttctcagctt tagcatttaa agttatgact gacccattcg taggtcgttt
     4621 aactttcttc cgtgtgtact ctggtgtttt acaatcaggt tcatacgtac aaaacgcaac
     4681 aaaaggcaaa cgtgaacgtg ttggtcgtat cctacagatg catgccaact ctcgttcaga
     4741 aatctctgaa gtttacgctg gagatatcgc tgctgctgtt ggtttgaaag acacaactac
     4801 tggggacaca ctatgtgatg aaaagagtct agttattctt gaatccatgg aattcccaga
     4861 accagttatc caagtcgcta ttgaacctaa atcaaaagct gaccaagata aaatgggtgt
     4921 ggctttacaa aaactatctg aagaagatcc tactttccgt gctgaaacaa acgtggaaac
     4981 aggcgaaaca atcatcgctg gtatgggtga acttcactta gatatcatcg ttgaccgtat
     5041 gcgtcgtgaa ttcaaagttg aagctaacgt aggtgcgcct caagtatctt accgtgaaac
     5101 tttccgtgct ggcactcaag ccgaaggtaa gtttgtacgt cagtctggtg gtaaaggtca
     5161 atacggtcac gtatggattg aatttacacc aaatgaagaa ggtgctggtt tcgaatttga
     5221 aaatgcgatc gtcggtgggg ttgttcctcg tgaatacatt ccagctgttg aaactggttt
     5281 gaaagatgct atggaaaatg gtgtcttagc tggctatcca ttagtagata tcaaagctaa
     5341 actttacgat ggttcatacc atgatgtcga ttcaaatgaa actgccttcc gtgttgctgc
     5401 ttctatggca ttacgtgcag cagctaagaa agctaatcca gtaatccttg aaccaatcat
     5461 ggctgtagaa gttgttatcc ctgaagatta tttaggtgat gttatgggtc acgtaactgc
     5521 tcgtcgtgga cgtgttgaag gtatggaagc tcgcgctggc ggccaacaag ttgttcgtgc
     5581 gatggttcca cttgctgaaa tgtttggtta tgctacaact cttcgttctg ctactcaagg
     5641 tcgtggtaca tttacaatga catttgacca ctacgaagat gtaccaaaat cagtacaaga
     5701 agaaatcatt aagaaaaacg gcggaaaagc tgaataattt cttaaaaccg gatagtcctg
     5761 caaagagatt atccaaagag actatatttt taatatagtt tcgtgtaaaa taattatcga
     5821 tgatatgggc atgtggtgta ccttgtgtac atcatatacc tatcagacaa ataaaaaata
     5881 tattgtattt ttaggaggac attttaaaat ggcaaaagaa aaatttgacc gttctaaacc
     5941 acacgttaac attggtacta tcggacacgt tgaccatggt aaaactacat taacagctgc
     6001 aatcacaact gtactatcta agaaaaacgg cggccaagct atggcttacg atcaaatcga
     6061 tggtgctcca gaagaacgcg aacgcggaat cacaatctca acagctcacg ttgagtatga
     6121 aacagataca cgccactacg ctcacgttga ctgcccagga cacgcggatt acgttaaaaa
     6181 catgatcact ggtgctgctc aaatggacgg agctatcttg gtagtttctg ctgctgacgg
     6241 cccaatgcct caaactcgtg aacacatcct attgtctcgt caagttggtg ttccttacat
     6301 cgttgtattc ttgaacaaag tagacatggt tgatgacgaa gaattactag aattagttga
     6361 aatggaagtt cgtgacctat taacagaata cgaattccct ggtgacgatg ttcctgtagt
     6421 tgctggatca gctttgaaag ctctagaagg cgacgcttca tacgaagaaa aaattcttga
     6481 attaatggct gcagttgacg aatacatccc aactccagaa cgtgacaacg acaaaccatt
     6541 catgatgcca gttgaagacg tgttctcaat tactggacgt ggtactgttg ctacaggtcg
     6601 tgttgaacgt ggacaagttc gcgttggtga cgaagttgaa gttgttggta ttgctgaaga
     6661 aacttcaaaa acaacagtta ctggtgttga aatgttccgt aaattgttag actacgctga
     6721 agctggagac aacattggtg ctttactacg tggtgttgca cgtgaagaca tccaacgtgg
     6781 acaagtttta gctaaaccag gtacaatcac acctcataca aaattctctg cagaagtata
     6841 cgtgttgaca aaagaagaag gtggacgtca tactccattc ttcactaact accgtccaca
     6901 attctacttc cgtacaactg acgtaacagg tgttgttgaa ttaccagaag gaactgaaat
     6961 ggttatgcct ggcgacaacg taacaatgga agttgaatta atccacccaa tcgctatcga
     7021 aaacggtact aaattctcaa tccgtgaagg cggacgtaca gttggtgccg gtgtcgttac
     7081 agaaatcaaa gcttaattca cttaagtttt aaagtaaagc aacatttatt cggacgtaat
     7141 cgctcggaac gaaggtctta ggatctttgt tccgagcttt tttatttatt gcaggatcag
     7201 gaaaaaagac aggagtatta taaatagaag aaacacgaaa taaaagagtc agtattttct
     7261 aaatgaaact ttatgaaaac aaaatgtttt ttacaagaaa aagccttgct ttgtgagttt
     7321 atatatatta taatgacaaa ggtgcgttta ctttagtgaa ttgcacagac gtaatcagaa
     7381 tgatttcgtc ggctaagaaa cgagaggttg cgacacgccc ggacgctttg ccacgaacga
     7441 gcgtgacgga aattttcgtg gagctatgtc tacttttcaa aataggcgaa ggagggaaca
     7501 agatggcaaa acaaaaaatt cgtatccgtt taaaagcgta tgaacaccgt attttagatc
     7561 aatcagcgga taaaatcgtg gaaactgcaa aaagaactgg agctagcgtg tctggtccaa
     7621 ttccattacc aactgaacgc agtctttaca caatcatccg tgcgactcat aaatacaaag
     7681 attcacgcga acaattcgaa atgcgtacac acaaacgtct tatcgacatt gtgaacccaa
     7741 caccaaaaac tgttgatgct ttgatgaagc tagacttacc atctggtgta aacatcgaaa
     7801 tcaaattata aaactaaaac aaaaataatg gaggtgtact catgaccaaa ggaatcttag
     7861 ggaaaaaagt gggaatgaca caaatcttca ctgaatctgg tgaattaatt ccagtaactg
     7921 ttgtagaagc tactccaaac gtagtactac aagtgaaaac aatggaaacg gatggctacg
     7981 aagctattca agttggttac caagatatgc gtgaagtttt atcaaacaaa cctgcgaaag
     8041 gtcatgttgc aaaagcaaac acggctccta agcgcttcat tcgtgaattc aaaaatgttg
     8101 agctaggaga atatgaagta ggaaaagaaa ttacagtaga tgttttccaa gcaggagaca
     8161 ttattgatgt aacaggtact acgaaaggta aaggattcca aggggttatc aaacgccacg
     8221 gacaaagccg cggaccaatg gctcacggtt ctcgttacca tcgtcgtcct gggtcaatgg
     8281 gtccagttgc acctaaccgt gtatttaaaa acaaacgtct tgctggccgt atgggtggca
     8341 accgcgtaac tatccaaaac ttggaagttg tacgtgtcga tgcagaaaga aatgttatct
     8401 tgatcaaagg taatgttcct ggagctaaaa aatctttaat tactatcaaa tcagctgtga
     8461 aagctaaata attgtaggaa gaaaggagga actaaggaat gccgaatgta gcattattca
     8521 aacaagatgg aagccaaaac ggtgaaatca cacttaacga agaaatcttc ggaattgaac
     8581 ctaatgaaag tgttgtttac gatgcaatcg taatgcaacg tgcgtcatta agacaaggaa
     8641 cacacgcggt taaaaaccgt agcgctgttc gcggcggcgg acgtaaacca tggcgtcaaa
     8701 aaggaacagg tcgtgcacgt caaggatcta tccgctcacc acaatggcgt ggaggtggcg
     8761 tagtctttgg accaacacca cgttcttaca gctacaaact tcctaaaaaa gttcgtcgtt
     8821 tagcaatgaa atctgtattg tcagaaaaag ttgctgaaaa caacttggta gcaatcgaag
     8881 gcttgaactt cgacgcacca aaaacaaaag aattcaaaca agttcttgct aacttatcta
     8941 tcgactctaa agtattggtt gttctagaaa aaggtaacga ctttgctgct ttatcagcac
     9001 gtaacttacc aaacgtttct gtagtaactt ctgataatgt gagtgtgcta gacgttgtat
     9061 caaatacaaa agtgttagca acacaaactg ctcttactca aattgaggag gtgcttgcat
     9121 aatgaactta ctagacgtaa tcaaacgccc agtgatcact gaaaaatcaa tgcttgccat
     9181 ggatgacaag aaatacactt ttgaagtgga cactcgcgca aacaaaactt tagtaaaaca
     9241 agcggtagaa gctgcttttg acgttaaagt caaaaacgtg aacatcgtga acgtacgtgc
     9301 gaaattcaaa cgtatgggca aatatgcagg atatacaaaa aaacgtcgca aagcgatcgt
     9361 tacattgact gaagattcaa aagaaatcca attattcgaa gctgctgaat aagcactaat
     9421 agataactca ttaaattagg agggaaaaac gtggcgatta aaaagtacaa acctacctca
     9481 aatggtcgtc gtaacatgac aggttctgat tttgctgaaa tcacaacatc aacacctgaa
     9541 aaatcattgt tacaaccatt gaaaaaccat gctggacgta acaacaacgg ccgcatcact
     9601 gtacgtcatc aaggtggcgg acacaaacgc caatatcgcg tgatcgactt caaacgtaac
     9661 aaagataacg ttgttgcaac tgttcaaacg atcgaatatg atccaaaccg ttcagctaac
     9721 atcgcgttag tacactatga agatggagtg aaagcttaca tcttagcacc taaaggacta
     9781 gaagtgggta tgaaacttgt ttcaggtcca gaagcagata tcaaagtcgg aaatgctctt
     9841 ccattagaaa acattccagt tggtactgtt atccacaaca ttgaaatgaa acctggtaaa
     9901 ggtggacaat tgatccgttc tgctggtaca agtgctcaag tacttggtaa agaaggcaaa
     9961 tacgtattga tccgcttgaa ctctggtgaa gttcgtatga tcttagctgc atgccgtgct
    10021 acaatcggtt ctgtaggtaa cgaacaacac gaattaatca acatcggtaa agctggacgc
    10081 tctcgttgga tgcgtaaacg cccaactgta cgtggtagcg taatgaaccc taatgatcac
    10141 ccacacggtg gtggtgaagg taaagctcct atcggacgta aagcaccagt ttctccttgg
    10201 ggtcaaccag ctcttggtta caaaactcgt aacaaaaaag ctaaatcaga caaacttatt
    10261 gttcgtcgtc gcaaaaataa ataattactt tgaccatgtg tcgcacattt tgagaggagg
    10321 ttcaccatgg gtcgtagttt aaagaaagga ccttttgtcg atgagcattt aatgaaaaaa
    10381 gttgaagctc aagcaggggt tgaaaagaaa aaagttatca aaacttggtc acgccgttca
    10441 acagttttcc caagttttgt tggatatacc atcgcagttt atgatggacg taaacatgta
    10501 ccagtttaca tccaagaaga catggtagga cacaaattag gtgaatttgc acctacaaga
    10561 acttatcgtg gacatgctgc tgacgataag aaaacgaaac gctaatttga ggggaggata
    10621 aatccaaatg gcagaacaaa tcacatcagc taaagcaact gcaaaaacag ttcgcacttc
    10681 acctcgaaaa gcccgtttag taattgacct tatcagaggt aaaagtgtcg cagatgcaat
    10741 ttcaatcttg aaattcacac caaacaaatc tgctggaatc attgaaaaag tattgatgtc
    10801 agcagttgcg aatgcagaaa ataactttga cttagatgtt gaaaacttgg tagtaagcga
    10861 agcttttgtt aacgaaggac ctacaatgaa acgtttccgt cctcgtgcaa aaggatcagc
    10921 ttctccaatc aacaaacgta caagtcatat cacggtagtc gtatcagaaa aataaggagg
    10981 gataatcagt gggtcaaaaa gtacatccaa ttggaatgcg tgtaggcatc atccgcgact
    11041 gggatgctaa atggtatgct gaaaaagagt atgctgagtt cttacacgaa gatttaagaa
    11101 tccgtaaatt tatttcaact aaacttgctg atgctgctgt atcaacagtt gaaatcgaac
    11161 gcgcaacagg tcgcgtaaac atttcaattc acacagctaa accaggtatg gttattggta
    11221 aaggcggttc tgaagtcgaa agcctaagaa aagaattaaa caaattaact ggtaaaagaa
    11281 tccatatcaa tatcgtggaa atcaaaaaac cagatctaga tgcaaaatta gtaggtgaag
    11341 gaatcgcacg tcaattagaa aaccgtgttg ctttccgtcg cgctcaaaaa caagccatcc
    11401 aacgctcaat gcgtgctggc gctcaaggga tcaaaactca agtatctggt cgtctaaacg
    11461 gagcagatat cgctcgttca gaaggatact cagaaggaac tgttcctctt catactttac
    11521 gtgcggacat tgattacgca tgggaagaag cagatacaac atacggaaaa ctaggagtta
    11581 aagtgtggat ctatcgtgga gaaattcttc caactaaaaa aaacactgag aaaggaggga
    11641 aataatcatg ttagtaccta aacgtgtaaa acaccgtcgt gaattccgcg gaaaaatgcg
    11701 cggggaagct aaaggcggaa aagaagtagc attcggtgaa tacggtttgc aagctgttga
    11761 ttcacattgg atcacaaacc gccaaatcga agctgctcgt atcgcaatga ctcgttacat
    11821 gaaacgtggt gggaaagtat ggattaaaat tttccctcac aaatcttata ctgccaaagc
    11881 aattggggta cgtatgggtt ctggtaaagg ggcacctgaa ggatgggttg caccagtaaa
    11941 acgtggtaaa atcatgtttg aaatcgcagg cgttcctgaa gaagtagctc gtgaagcgtt
    12001 acgtctagct tctcacaaat taccaatgaa aactaagatc gtaaaacgtg aggaaatggg
    12061 tggtgaatcg aatgaaggtt aaagaaatca gagaattaac cactgccgaa atgcttgatc
    12121 aagaaaaaca attaaaagaa gaattgttta atcttagatt ccaattagca acaggtcaat
    12181 tagaaaacac tgcacgtatt aaagaagtac gtaaatcgat tgcacgcatc aaaactgtgt
    12241 tgcgtgaaca agctaagtaa ttatggaagg aggccattaa gcgtatgact gaaggaagaa
    12301 atcaacgcaa agtttatcaa ggtcgcgtgg tttcagacaa aatggataaa acaattaccg
    12361 ttgctgtcga aacaaagaaa aaccacccta tctatggcaa acgcatgaac tattctaaga
    12421 aatacaaagt gcatgatgaa aacaacacag caaaagttgg cgacatcgtg agaattatgg
    12481 aaactcgtcc attatcagct acaaaacgtt tccgtttgtt ggaagtagtc gaagaagcag
    12541 ttattatcta ataacacgag attttcctat atagattgaa tgcaaaatct gaaaggagga
    12601 tacacaacgt gatccaagca gaaagtcgtt taaaagtcgc tgataactca ggcgcacgtg
    12661 aaattttgac gatcaaagtc cttggtggat ctggtcgtaa aactgctaac atcggtgatg
    12721 tgatcgttgc tacggttaaa caagcaacgc caggtggggt tgtcaaaaaa ggtgacgtcg
    12781 ttaaagccgt tatcgttcga acaaaatcag gagctcgtcg tacagacggt tcatatatta
    12841 aatttgatga aaatgctgca gtgattattc gtgatgataa aagccctcgt ggaacacgta
    12901 tctttgggcc agttgcacgt gaactacgtg aaaacaactt catgaagatc gtttcactag
    12961 caccagaagt tttataatct tgagacactt atcaaaggag gtgcgaaaca gttatgtttg
    13021 ttaaaaaagg cgataaagtt aaagttatca ctggaaaaga caaaaacaaa gaaggcgttg
    13081 tactagcagc gttcccgaaa aaagataaag ttattgtcga aggtgtaaat gtcgttaaaa
    13141 aacaccaaaa accttctcaa gctgctcctc aaggtggaat tgttgagatg gaagcgccaa
    13201 ttcatgtttc taacgtgatg gtggttgact ctactggtgt agctggccga gttggctaca
    13261 aagaagtaga cggcaaaaaa gtccgtgttt ctaaaaaaac cggtgaagtt ttagataaat
    13321 aaaagtttaa ggaaggaggg tcttactgaa tgaaccgcct aaaagaaaaa tatcttaaag
    13381 aagtaactcc atcattgatg gaaaaattta actatagctc tgttatgcaa acacctaaag
    13441 ttgaaaaaat cgtcatcaac atgggtgttg gtgatgcggt atcaaacgct aaaaacttag
    13501 ataaagcagt tgaagaatta actttgatca ctggtcaaaa accaatgatt acaaaagcta
    13561 aaaaatcaat cgctggattc cgtttacgtg aaggaatgcc aattggtgca aaagttacat
    13621 tgcgcggaga aagaatgtac gaattcttag acaaattggt ttctgtttca ctaccacgtg
    13681 tacgtgactt ccatggtgta agtaaaaaag cctttgacgg tcgtggaaac tatactttag
    13741 gtattaaaga acaattgatc ttcccagaag tagattatga tttagtagat aaagtacgtg
    13801 gtatggacat cgttattgta acaacagcaa actctgatga agaatctcgt gagttgttga
    13861 cacaattagg catgccattc caaaaataat taaaggaggc gaactacgtg gctaaaaaat
    13921 caatgattgc taaaaacaaa cgtcctgcaa aattttcaac acaagcttat actcgttgcg
    13981 aacgttgcgg acgtccacat tcagtttatc gtaagttcca tctttgccgt atttgcttcc
    14041 gcgaacttgc ctataaaggt caaattcccg gcgtgaagaa agctagctgg taaaaagtaa
    14101 attcgcataa gaaggaggta aaagatcaat ggtcatgaca gatccaattg cagattttct
    14161 aactcgtatc cgtaacgcaa acatggttaa acatgagtct ttagaagtac ctgcatcaaa
    14221 aattaaacgt gacatcgctg aaatcttgaa acgtgaaggt ttcgttcgtg atgttgaata
    14281 catcgaagat gacaaacaag gcgtgatccg tgttttccta aaatttggta aaaacggcga
    14341 acgtgttatc acaaacttaa aacgtatctc taaaccaggt ttgcgtgctt atgtcaaagc
    14401 tgacgaagta ccaaaagtat tgaacggttt aggtatcgca atcatttcga cttctgaagg
    14461 tgttgtcact gataaagaag ctcgtgaaaa aaatatcggc ggcgaagtta tcgcttacgt
    14521 atggtaatca agtagaatct acaaggaggt gtctttaagt gagccgtatc ggaaataaag
    14581 tcgttgttat ccctgaagga gtaacagtta ctcaagacgg aaacaacgtt acagttaaag
    14641 gaccaaaagg tgaattgact cgtacttttt cagcagatat caaaatgaat atcgaaggaa
    14701 acgaagtaac tttcactcgt ccaaacgaca gtaaagaaat gaaaacaatt catggaacaa
    14761 ctcgtgcgaa cttcaacaac atggttgtag gtgtcagcga aggtttccaa aaagcgttag
    14821 aactaatcgg ggttgggtac cgtgcacaat tacaaggatc aaaacttgta ttgaacgttg
    14881 gttactcaca tccagtagaa atcacaccac cagctggagt aactgttgaa gtaccagcta
    14941 acacacaagt aattgttaaa ggtgcgaaca aagaagaagt tggcgaatta gctgcaaaca
    15001 ttcgtggaac tcgtcctcca gaaccttata aaggtaaagg tattcgttat gttggcgaat
    15061 atgtacgccg taaagaaggt aaaactggta aataatcgtt tagaaaaatt gcatagaagc
    15121 cagcagcagg aaaaaggcat ttggctgaag tttaccttca aaaaaataca tttttacttg
    15181 tgccgcagct tctatttttc gaaacgtatt tactgcttga aatagtagta caataaaaat
    15241 aaagaggtga caattgtgat tacaaaacca gataagaaca aaacacgtca aaagagacat
    15301 cgtcgtgtac gtaacaatat ctctggtact gctgagcgcc cacgcttgaa cgtttttcgt
    15361 tctaacaaaa acatctacgc tcaaattatt gatgacgtag cgggtgtaac gctagcaagt
    15421 gcctctacct tggataaaga aatttcaggt ggaacaaaaa cagaagcagc acaagctgtc
    15481 ggtaaactag ttgctgaacg tgcagctgaa aaaggcatta aagtagtagt ctttgaccgt
    15541 ggtggatacc tttaccatgg ccgtgtgcaa gctttagctg aagctgctcg cgaaaatgga
    15601 ctagaatttt agaagaagga ggaataccat tcatggttta tatcgatcca aaacatttgg
    15661 aattagaaga ccgcgttgtt gcaattaacc gtgtaacaaa agttgttaaa ggtggacgtc
    15721 gtctacgttt tgctgcttta gttgttgtcg gtgacaaaaa cggacacgta gggtttggta
    15781 caggtaaagc tcaagaagtt cctgaagcaa tccgtaaagc aattgaagat gcgaagaaaa
    15841 acttagttga agtacctatg gttggttcaa caatcccaca cgaagtaatc ggtgctttta
    15901 gtggtggtag aatcctaatg aaacctgctg tagaaggttc tggggttgct gctggtggac
    15961 cagttcgtgc cgtattagaa ttagctgggg tagcagatat cacttctaaa tctttaggct
    16021 caaatacacc aatcaacgtt gttcgcgcaa cagttgaagg tttgaaacaa ttgaaacgtg
    16081 cagaagaagt tgcagaactt cgcggcaaat cagttgaaga attaatcgga taaggaggac
    16141 aacaataatg gctgaattaa aagttacttt aaaacgcagt attatcggac gtcctcaaaa
    16201 ccaacgcgat acagttaaag cgttaggtct tacaaagatt aacagttcag tggttaagcc
    16261 ttctaatgaa gcaattaaag gcatgatcaa tactgtttct cacctagtgg acgttgaaga
    16321 aatttaattt agaaaaatca taactagaac attaaggagg tgccaaacct atgaaacttc
    16381 atgaattaaa acctgctgaa ggttcacgcc aagtacgtaa ccgtgttggt cgtggtactt
    16441 catctggtaa cggaaaaact gccggacgcg gacaaaaagg acaaaaagct cgttcaggcg
    16501 gcggtgtgcg tttaggattt gaagggggac aaacaccatt gttccgtcgt ttaccaaaac
    16561 gaggattcac aaacgtgaac cgtaaagact atgcagtcat taatcttgat gtattgaacc
    16621 gctttgaaga cggagctgaa gtaactcctg ttgctttagt tgaagctgga atcgtgaaaa
    16681 acgaaaaagc tggaatcaaa gtattagcta atggggaact aaacaagaaa ttaactgtga
    16741 aagcagcaaa attctctaaa gcagctcaag aagcaattga agcggctggt ggatcaatcg
    16801 aggtgatcta atgtttagac tattaaaaga tgcttttaaa gtaaaagaca ttagatcgaa
    16861 aatcctattt actgtatttg tgttgtttgt atttcgccta ggtgcacata ttactgtacc
    16921 aggcgtaaat gctgcttcat tgcaaaacct ttcttctttg ccatttttca acatgttgaa
    16981 cttggtaagt ggtagtgcga tgcaaaactt ttccattttc tcaatggggg tttcaccata
    17041 cattactgct tctatcgtca ttcagcttct tcaaatggac atcgtgcctc gatttgttga
    17101 atggtcgaaa caaggggaag ttggtcgtaa aaaactgaat caagcgactc gatatctgac
    17161 attagtattg gcatttatcc aatctgtcgg tatcactgct ggattcaatc aatacgctca
    17221 attaggtttc gtgaataatc ctaacgtagc aacttacgta acgatcggtt tgattctaac
    17281 agccggtacg atgtttgtta cttggctagg agaacagatc acagaaaaag gtatcggaaa
    17341 tggcgtatca atgatcattt ttgcaggtat catttctaga ttgcctgaaa gtatcagaga
    17401 aatttatgaa gattacttta taaatattga tccttcagac atatggaaat ctgctatttt
    17461 tgtggccatt ttactagtag cggtcttgtt gatcgtgaca ttcgtaacat ttttccaaca
    17521 agctgaacgc aagatcccga tccaatatac gaaacgcgta gctggcgcgc ctacaagcag
    17581 ctatctgccg ttgaaagtaa atgcagctgg agttattcca gttatctttg ctagttcgtt
    17641 tattgctacg ccaaatgctg ttttacaagc attgagcggt agattcggtt ctgaaaactg
    17701 gtacaaagtg atgatggaga tctttaacta taatacagca cctggtgcta cgatttatac
    17761 gattctgatc gttgccttca ctttcttcta cgcctttgtt caagtaaacc ctgagaaatt
    17821 agcggaaaac ttgcaaaagc aaggaagtta tataccaagc gtacgccctg gtaaggggac
    17881 tgaagagtat gtctcaagat tactgatgcg tttaagtaca gttggtgcag tcttccttgg
    17941 tttagtggca ttgcttccaa tcattgctca aatggtttgg gatcttccac aatcaatcgg
    18001 attaggcggt acaagcttac tgatcgttat cggtgtagct ttagaaactg cgaaacagtt
    18061 agaaggctta atgctgaaac gtaaatatgt tggattcatt gagtaagaaa gcgtgttgag
    18121 gtttcccctc aacacacgtt ctcacacatt tattaggagg gaaaacaatg aacctcattt
    18181 taatggggct gccaggcgca ggtaaaggaa cacaagctga aaaaatcatt gatgcttatg
    18241 gtattccgca catctcaaca ggagatatgt tccgcgcagc gatgcaaaac gaaacagctc
    18301 ttggcctgga agcaaagtct tatatggata aaggtgcatt agttcctgat gaagtaacaa
    18361 atggaatcgt gaaagagcga ctagctgaac cagatacaga gaaaggcttc ttattagatg
    18421 gctttccacg tacgctagat caagcaaaag ctctggacgc aatgctaaaa gatttgaata
    18481 aaaaaattga tgctgtcatc gatatccatg ttggtgaaga aatcttggtc gaacgtctag
    18541 ccggccgatt tatctgcagc aattgtggag caacttacca taaaattttc aaccctacaa
    18601 aagttgaaga cacatgtgat cgttgcggtg ggcatgaatt ctatcaaaga gaagatgata
    18661 aacctgagac ggttaaaaat cgtctggcta tcaacatcaa aaatagtgaa ccaatcttgg
    18721 cttattataa agaacaaggt ttgctaaaca ctattgatgg tgctcgtgaa atcgatgctg
    18781 tatttgcaga tgtacaaaaa atcattgaaa aatagcacga ttgcccgtag ccgacttgca
    18841 ttatgcagtt actcttgtca agttcaataa attatgatag aatatgtcag tccgacttct
    18901 agagaatcct ctagatgatc cattttgaga attttgaggt gggaactgca gtttccgcca
    18961 ttttatcgtg tgaaaatgcg aaacaagtac aaggaggtac tgtgcgtggc aaaagaagat
    19021 atgattgaag tcgaaggtac agtcgtcgaa actttgccga atgcaatgtt taaagttgaa
    19081 ctagaaaacg gacaccaagt tcttgctact gtttcaggta aaattcgtat gcattacatc
    19141 cgtatcttac ctggtgacaa agtaacggtt gaattgtctc catacgatct taaccgtggc
    19201 cgtattactt atcgctttaa ataattgtac tccgtaaaac tacaggaggt attatcatga
    19261 aagtaagacc atcagtaaaa ccaatgtgcg aacattgtaa agtaattcgt cgtaaaggac
    19321 gcgttatggt gatttgccca gcaaatccaa aacataaaca acgtcaagga taattggagg
    19381 tgctataaat atggctcgta ttgcaggagt agatattcct cgtgataaac gtgtagtagt
    19441 ttcacttact tatatctatg gtattggtaa cactactgct aaaaagatct tagctgatgc
    19501 tggtgtatct gaagatgttc gcgttcgtga attaacgaat gaacaaacag atgctatccg
    19561 tgcagaagta gataaattaa aagttgaagg ggatcttcgt cgtgaagtga acttgaacat
    19621 caaacgtttg atggaaatcg gttcttaccg tggaatccgt catcgccgtg gattgcctgt
    19681 tcgtggacaa aacacgaaaa acaatgcacg tactcgtaaa ggcccagctc gcgctatcgc
    19741 tggcaagaaa aaataattct taagtgaagg aggttaaaac ttcatggcag caaaaaaagt
    19801 gaatcgtaaa cgccgtgtga agaagaatat agaagcaggt attgcacata tccattctac
    19861 attcaataac actatcatca tgatcactga tactcacgga aatgcattag cgtggtcatc
    19921 agctggttca ttaggtttca aaggaagcaa aaaatcaact ccttttgccg ctcaaatggc
    19981 agcagaaact gctacaaaag cagcaatgga acacggatta aagactgttg aagtaacagt
    20041 taaaggacct ggttctggac gtgaagcagc aattcgttca ttacaagcta caggtttaga
    20101 agtgactgca attcgtgacg tgactccagt tcctcataat ggtgcccgcc ctccaaaacg
    20161 ccgtcgtgtt taatgagtgc cgctcatttt gagttttatc agtaacgtca ttgaacaaga
    20221 cacttttcgt tttgaaaggg gtaaagataa gaatgattga gttcgaaaaa ccaagaatcg
    20281 caaaaattga tgaagaaaaa gattatggca agttcatcgt tgaacctctt gaaagagggt
    20341 atgggactac tttagggaat tccctacgtc gtattttatt atcttctttg ccaggtgctg
    20401 ccatcactaa tattcaaatc gatggtgtac tgcatgaatt ctctaccatc aaaggtgtta
    20461 gagaagacgt cactcaaatc atcttgaaca ttaaaggctt agcgctgaaa atgtatggcc
    20521 aagaagaaaa aacccttgaa atcgatatta ccggacctgc tacagtaact gctggcgata
    20581 ttatcgttga tagtgatgta gaaattctta acaaagatat gtacatctgt agtgtctctg
    20641 aaggagctac gttccatgct cgcttaacag tgaaacctgg acgtggttat gttcaagcag
    20701 atgaaaataa aaaagaagat atgccaattg gcgttcttcc agtcgattca atctacacac
    20761 ctgttcgtcg tgtgaactat caagttgaga acacacgtgt tggacaccgt gaagattttg
    20821 ataaattgac gatggagatt tggacagatg gttcaattga accgttggaa gcaatgagtt
    20881 tgtctgcgaa aatcatgact gaacatttgg atatttttgt gaacttaact gatgaagcaa
    20941 aaaatgctga aatcatggtc gaaaaagaag aaacacaaaa agaaaaaatg ttggaaatga
    21001 cgatcgaaga gttagacttg tctgttcgct cttacaattg tttgaaacgt gcaggaatca
    21061 atactgtaca agaacttaca aataaatctg agcctgaaat gatcaaagtc cgtaacttag
    21121 gacgtaaatc attagaagaa gttaaagcaa aattgcacga cttaggttta ggtttacgta
    21181 aagaagatta aaacctttta cgaaggaggg aaaccaacgt gagttatcgt aaactaggac
    21241 gcacatctag ccaacgtaaa gcgatgttgc gtgatttaac aactgattta ttaatcaacg
    21301 aacgtattgt gactactgaa gctcgtgcga aagaagttcg ctcaactgca gaaaaaatga
    21361 tcactttagg taaacgcgga gatttgcatg cacgtcgtca agctgcggct ttcgtacgta
    21421 atgaagttgc cagcgtgcgt gaagaaaatg aggacatcgt cattgaatca gctttacaaa
    21481 aactgttcaa cgatattgca cctcgttacg cagaacgtca aggcggatac acacgcattt
    21541 tgaagacaga accaagacgc ggtgatggtg caccaatggt tgtcttagaa cttgtctaaa
    21601 atcacactaa taacatcact ttatagatga ttcttttaga gtgttatgat gttggaagat
    21661 atttttccga gtctagctca gcgctgctcc tcaagcgaat atcgcttggg gagtaagcac
    21721 tcatcataaa gtgttttttt ataccttcaa ttacctgtca tgacagatat gtttgaaggc
    21781 ataaaaaaac attttaagcg agaaataatt gaaaattttg tctttttttg atagtgttag
    21841 taggaacttt aggatgaaga aggagtaatc atggagccaa ttattgaatt agaaaagata
    21901 aattataaat accagccaga tgatcttcgc cccgcattga aagatgtctc ctttacaatt
    21961 gataaaggtg agtggatcgc tattattgga cacaatggtt caggaaaatc gacacttgcc
    22021 aaaacgatca atggattgct tctgccagaa tcagggatcg tgaaagtggg aaatcaaata
    22081 ttggatgaag aaaatatttg gacgatccga cagatggttg gaatgatttt tcaaaatcca
    22141 gacaatcagt ttgttggttc gacagtagaa gatgacgttg cttttggtct tgaaaatcaa
    22201 gggattccca gagaagaaat gctagtgcgt gtgaaagatg cacttgagaa agttagaatg
    22261 gctgagtttg cttcacgcga gcctgctcgt ttatcaggag ggcaaaaaca acgtgttgcc
    22321 attgccggcg tggttgcctt acgacctgat atcattattt tagatgaagc aaccagtatg
    22381 ctagatccag aaggtcgtga agaagtgatc tctacgatca agaagatcaa agaagaaagt
    22441 caattgaccg ttatatctat cactcatgat attgatgaag cagctaatgc caatcggata
    22501 ctagtgatga gacaaggcga acttgtccgt gaaggtacac caaaagaaat tttttctgct
    22561 ggtcctgaac tgatcgactt aggtctagat ctaccatttc ctgaaaaact gaagagcgca
    22621 ctgaaagaac gtggggttga tgtgcccagt gaatatatga cagaagaaag gatggtggat
    22681 tggttatgga catccgtttt gaacaagtag acttcactta tcagccgaat acaccatttg
    22741 aacaaagagc cttatttgac atcaatatga cgatcaaaga aaacagttat actgcattag
    22801 tagggcatac cggaagcggg aaatcaacct tacttcagca tttgaatgcg cttgttaagc
    22861 caacaagcgg tacagtccat attggagaac gagatattca gccggatacg gataataaga
    22921 atttaaagcc catccgaaaa aaagttggca tcgtctttca gtttccagaa gcacagctat
    22981 tcgaagaaac ggtagcaaaa gacattgctt tcggtcctaa aaactttggt gtcagcgaag
    23041 aagaagcact agtcctagca aaagaaacat tagaacaagt tgggctggat gaaagctatt
    23101 tggaacgttc gccatttgaa ctttcaggag ggcaaatgcg tagagtagca atcgctggtg
    23161 tgcttgccat gaggccggaa gtacttgtat tggatgaacc aacggcagga cttgatcccc
    23221 agggcagaaa agaaatgatg gagatgtttt ggcgtcttca taaggaacag cagatcacta
    23281 ttgtattagt tacgcattta atggatgatg tagccaattt tgcagattat gtctatgtgt
    23341 tggaaaaggg acgaatcgtt aatagcgggg agccgcaaga ggtctttcaa aacattgaat
    23401 ggctaaaaga aaaacaacta ggcgtaccta ctgccaccga gtttgcagaa gaattaatgg
    23461 ctaaagggat gaactttgct accttgcctt tgacagctga agaattagca gacgctatcg
    23521 tccattatgc tggaggaaat gcccatgatg aataaattga tatttggtcg ttatatgcct
    23581 ggagattcct tgatccatcg attggatccc cgagctaaat tattagccag tttttatttt
    23641 atcggaatca tttttttagc aaataattgg gaaacgtacc ttttgttggc atttttcaca
    23701 ttactttctg tcttcttgtc aaaagtagat ttgaaatttt ttatccgagg cattcgccca
    23761 ttgatctggc taatcctctt cactgtagct ttgcaggtct tgtttacaag tggcggagaa
    23821 acttattgga gttggggtgt tttccatatc actgaatttg gtgtgatgaa cggcctcttt
    23881 attttctgtc gattcgtgtt gatcattttt atgtcgactc tactcacgct tactacaccg
    23941 cctttggaat tatcggatgc gattgagtac ttgcttcgtc cattgaaagc cgtacggttc
    24001 ccagtccacg aaatttcatt gatgctgtcg attgctcttc gttttgtgcc tacattgatg
    24061 gacgaaacag aaaagatcat gaacgctcag cgcgctcgcg gtgttgattt tggtgaagga
    24121 aacctagttc agaaaatgaa agcagtcatt cctttgctga ttcctttatt tgtcagcagc
    24181 ttcaaccgtg cggaagatct tgcgactgct atggaagcaa gaggctacaa aggtggggaa
    24241 ggaagaacga aatatcgtgt attgcattgg cataatagag acacgatggt tatcattgct
    24301 tttgtattat taactgtgct actgatcttt ttaagaggat aaacgttcag tgggccgcta
    24361 tctattcttt tcttgctgat ctgtttgcaa gaaaggatgc aagcagtgta tgacaagaaa
    24421 tattagggtc tgtggcagac taaggtcaca ggcctctttc ttttatctga aaagaaagaa
    24481 cgaaaaaatt ttatagaaaa atcattattc cagataacct tttaggaaca aaggagagaa
    24541 aaacatggta cgttacaaag caatcattgc gtacgacgga acaaatttta atggattcca
    24601 aaaacagcca aatgggcgta ctgttcaaga agaagtagaa aaaacattac aaaaaatggc
    24661 aaatggaaaa gaaatcacgg tctttggttc gggaaggaca gatgccggag tacatgcgat
    24721 cgggcaagta atccattttg attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc
    24781 gctagatacc cagtcaccag aggatattgc tgtaagacaa gtagaaattg tttcagaaga
    24841 cttccacgca agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa
    24901 accacgcagt ccttttcgac gccattatgc aagttatttt ccttacccac ttgatttgga
    24961 aaaaattcag cgggcgcttc ctgatttatt agggactcac gatttcacct ctttttgtgc
    25021 atcaggaagc tcgatagagg ataaaatacg tacgatctac gaggctaaga tggaggtaaa
    25081 tgaaacaaaa gacgaattgt tgtttacatt taggggaaat ggcttcttgt ataagatgat
    25141 acggatctta gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg aagatagtat
    25201 cccagatatt attgcgaaaa aagatcgtaa tgcagcagga cctacagcac accctgaagg
    25261 actttatttg tatgaagtga aatatgaata agaggttatg atagaagtgg tcagcttcaa
    25321 gaaataagaa ggtatttacg aaaattactt ctcaaaattt tgcaaaattt cggcttattt
    25381 tcgaagaagc tacttctgga acaccgttta agaaagaggt cgtgagcctg tcatttaact
    25441 ctgagtcaca aagagcaatt tcaaaaaaca gcttctcatc tttgatgaat atcgtgactt
    25501 gtggacgagg cgttgataag tgaatcatct ttttgttacc tattttttct gtaaccaaaa
    25561 aacgatggaa cgttgttttg attgcccttg gtgcggacag tctgaacagg ttccatcgtt
    25621 tctttatttt ctacgtcttc ttctgtgtgt gtttcgtggt tgttcttcta ttgctggatc
    25681 aatcgtgcca taaaaggggc gattgcggtc aaggtaccag acataaagaa taaacaatgc
    25741 aatacatagg acaaaaagga ttgcacctat cgtaaatcct tcaggcaagt aactgaaagt
    25801 gacagtatgt ttacctgctg gaacttttat catcaagaat gcgtctttga aattgctgac
    25861 cttgactggt ttcccatcaa ttttagcttt ccatcctttg tcataaggaa tagttgtcac
    25921 caacatggaa tcttcagcgg tatttacagt cccagaagct tttctgccag tggcttccat
    25981 ttcgacacct ttttctttga tctgattcat tgcggcttga taggctacaa catctagacc
    26041 aacgacttta ggctccatga aacttatttc tttcgtccca taaaaacttg cagtaaatgt
    26101 cactgttgtc tcttgaggat aatagccaag atcatagtat tgaccagaaa tgttgatttg
    26161 cgtttttcgc gatgtaccat tgacagtgat cgtcgcacta gaactttcta attgcgcaaa
    26221 attggttggg aacaaactta agtaagcttg tgtatttgca ggcacatgga cttcccaagt
    26281 gagctctttt ggctggttat ctgctgtttc tgagtaagtg acgccagtcg ttgtctttgt
    26341 gatcgtcgta ttcaactgat tcttcaaagt aggtgtatag aatgtaaaat actgttcatt
    26401 tagaccagac aatgtattga tcaagtttgt ctgactggtc aaattatctg tcactggctg
    26461 ctcaacatca ttaacggaca acggtgcagt aaatgctagt ggcaatgcat tgacattctg
    26521 atacagtgta tagtcgccgc tttgaccagc ttggcggaag ccatatttac tgaacgtgct
    26581 ttcgttttta gaaatgttat atttgattcc tgtaaaccca tccatcagca aagtattatt
    26641 gttataacgg atattcaaat ttgtccctct tgaacggaaa cctaatttat ccaggtaaga
    26701 ggaagaatta cgatttctta tagaagaaaa caaactgata ccactgtaac catagttgat
    26761 cgaatcgttt gcagatactg gatccagatt ttccaagcgg taaaacgagt cggaatcttt
    26821 ttttgtttca tttacaagag ctttcaagtc aggataagga tcagtatata aactgcggga
    26881 agcataattc caatcatcaa gaatgccatg gatcatactg ttcgtattga ccagtgcttc
    26941 agcagacatg atcaataata acaatatact cagatgattg aatgaaagtt ttttatgctg
    27001 ataaaatccc acagcaaaag cataaatccc taagaaaacc actgtcaata caaaggaagt
    27061 catcgtcaca taggtgtagc ttccttcagg tttgatgccc catgccaccg caaagagagc
    27121 agctacgacc aaaatgattc ctaacaacag cccaccatcg tcttgttcta tcttttccca
    27181 tccttttcct gcaagcagga gcactaagaa agagaacaga taggcataac ggaataaaaa
    27241 catattcggt gcatgcatac catgccagaa caaattcaat ggaacaagat aaaaacttgc
    27301 gataagtatg gcgaaaagac tgccgtacaa aaatttattt ttcaacggta ctttccgact
    27361 ggcaaaataa aacagacaga ggactaatgg tagcagacct gcgtaaacga acggaatcga
    27421 tccgtatttt gtcgtgtcat aaacaccaat catatttttc atgattagat ccagataaga
    27481 agtcgcttct gttttcaacg tagtgatctg actcagtgcc tcgccatttg accgaagatc
    27541 cagcactgct ggcaatacaa tgatcattga agcgccgcct gccagtaaag aagtgatacc
    27601 ataaggaaca atcgtttttt tatattctga ccagtttgtc aatagacggg cgataaaata
    27661 taagaaagaa aatacgccaa tcataaaacc catataaaaa ctggataaaa acaagagtaa
    27721 ataactgata aataaaagtt tcggccgttt aagatccata acacgatgaa tccccaaaat
    27781 cactaatgga agataaacaa atgcatccag ccacatgatg atctccgaat gtgcagtgat
    27841 aaatgagatc aatgcgtaac tgacgctaag tgcaacatgc ccccagcgag gaatcttgaa
    27901 cgtacgtgca agaaaccaaa aagccaatcc ggcacagccg accttaagca aagtcagaac
    27961 atacagtgca tctggcatca tctggtttgg aaaaaagaac accagtggcg tgaagatccc
    28021 gcctagatag taagaaatta aagataaata attcaatcct agagaagcat tccatgtata
    28081 aaaaatactt tgctttccaa gaagaacatt tcggaagctg gcatgaaaat tagaaaactg
    28141 tgaaaaggca tcacttgcca aaatacttct gctgcttcca ggatagattc cgatacttag
    28201 ataaacaagt gctaaaatca aaaacggaat caggaaacta gctgctgcgt agcgtccgtt
    28261 ttctctaaag aatgacttta aatttttttt catatttatt ttccaccaaa tttctatgaa
    28321 gttgtattca gtttatcata tccttgtttt tctctgctta aaaagagtct agttttcaca
    28381 aaaaaataaa atttagaata aataacttca taatttgttc gtataacaaa aataaacgca
    28441 aaaaaagggt aggaatcaaa agtcccaccc ttaaaaattt ttattaaata aaaaaccgtt
    28501 cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc ctgatttcaa
    28561 agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc gaactttttg
    28621 taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt tgtttccact
    28681 ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct cctttgcctt
    28741 gatttggagc aatagaataa ttcagctcat accagactaa catatcatat agaatgggaa
    28801 tttgcaagta cattttcata gatacaattg acaattgaga agaataattg taaattaaac
    28861 aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc cttttatttc
    28921 aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc cgttaaggag
    28981 gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt accaatgatg
    29041 taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta ggaatggcca
    29101 gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat tactcaagag
    29161 gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg attatcagct
    29221 atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa tacaatctag
    29281 aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt gttcgtgtac
    29341 tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg aggattatct
    29401 aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag gcaggtgcga
    29461 atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct gtgccagatg
    29521 gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca gtagtagatt
    29581 caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg ctgatgggtg
    29641 cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct aagcaaattc
    29701 cagatgttgt ctcattaaat gcttctgatc ttgctgcagc cttcactcat ggagttgaaa
    29761 ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta gctagagaag
    29821 cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa gtaatgacag
    29881 ctgttgtaaa aggagcaaag cgtgctttag ctaaaactcc agacttgctt ccagtcctaa
    29941 aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat gagggctttt
    30001 taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct ccagcagaaa
    30061 tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg gcgacagaag
    30121 atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga cctacagtcg
    30181 atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga gattctttac
    30241 ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac ccaggagaag
    30301 tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac aacatgcgct
    30361 tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg aaaaaagaaa
    30421 taccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc caagagctgt
    30481 ttgaaagttt aggcgctaat tatgttatca gtggtggaca aacaatgaat ccaagtacgg
    30541 aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc ttgccaaaca
    30601 ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc ccagtagcag
    30661 ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc aatgatcaag
    30721 cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt gtgagcggtc
    30781 aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa gaaggagact
    30841 ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca gcaacagcgt
    30901 taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc ttggcaggtg
    30961 atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa aaacatcctg
    31021 atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta ttttctgctg
    31081 aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa ggccttcggt
    31141 tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taaaggaggg aggaaaaatt
    31201 tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa aaagagcagc
    31261 tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt atccttttcg
    31321 ctacgaagat attcaggaaa gaaatctaaa cgagatccag gatcaagaaa aggttacttt
    31381 gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa aaagccgatt
    31441 gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta atcaacctta
    31501 tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat gggatgcaaa
    31561 gcgtaaagcc ttgaacggga tgaagatttt aggttctcaa acaatggatg acttttcgcc
    31621 gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa tcaaaaaagg
    31681 gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg tggagaaata
    31741 tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag accacgaaga
    31801 aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc aaatgcgtct
    31861 tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc tttatgatgt
    31921 ccaaaaattg aaacaattta ctcaaaagct accttttgaa ctgacaaatg cacaaaaacg
    31981 tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc gacttttgca
    32041 gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg ctaccgttac
    32101 agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac aacatatgga
    32161 aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgatta cggggtcaac
    32221 gaaagcaaaa gaacgacgtg agctccttga aaaactagag caaggggaaa ttaacgtcgt
    32281 gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag gtctagttat
    32341 tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg aaaaaggctg
    32401 gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag ctattacagc
    32461 atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta tcccgatcga
    32521 aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga aaaaagaact
    32581 ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag aagcattaga
    32641 tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac caaaatatga
    32701 agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca tggaagcctt
    32761 caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag gggtaaacgt
    32821 gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg cacagctgca
    32881 ccaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct tagttgctaa
    32941 tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca ccaatggttt
    33001 tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg gcttccgtca
    33061 atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg ttttggaggt
    33121 agccagagaa gaggctgcag ccctttggca aataaaggat tggcagatgc ttcctgagta
    33181 tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt tcgattagca
    33241 gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga aaattgccgt
    33301 agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg ctttagcgca
    33361 gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa tcaagaaata
    33421 cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca atagtgatga
    33481 tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg ctgcacaagc
    33541 agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg cgctactagc
    33601 agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct tgatgtctac
    33661 cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg gcgctaacgc
    33721 agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt atgcacgtaa
    33781 agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag aagcaacaaa
    33841 aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa gcttgaattt
    33901 tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag tggtcacaga
    33961 tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga atatgatgaa
    34021 tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg ctatgctttt
    34081 aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg gcggtgccgt
    34141 tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc ccgatgctgt
    34201 tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg gacaattagt
    34261 cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcgatc cgttccattg
    34321 aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt aggtaattga
    34381 caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt aacaaagtag
    34441 cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat gaattgaaca
    34501 tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta gagcttgaag
    34561 atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca gtcggtggcg
    34621 cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag aaggataaaa
    34681 agctgcatgc agctttttat ccttcttttt ttaatctttt tgacacgtaa aagtcatttt
    34741 tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcggtt ttctatataa
    34801 ttattaataa ttcattacac aaagataaga ataacctttg ttataaaacg atttattctg
    34861 tgtaatgtcc ttttttatta agaaaaactt ccatttagga agcagagagg ggtattcgat
    34921 tgtccgataa ccaattatta gaagttcaga acctacatac aggattccga ctcaaagatg
    34981 actattacga tgcagtagat gacgtttcat ttactttaga caaaaacgag attttagcaa
    35041 tcgttgggga atctgggtgc gggaaaagta ccttggctac tacgattatg ggattacttg
    35101 atcctaataa cacaaaaatt acaggtgaga ttttatataa cgatctgaat ttaatcgacc
    35161 tgaacgaaac gctctataat aaaatccgcg gaaatgatat cggaatgatt ttccaagatc
    35221 cgttgtctgc attaaatcct ctcatgagaa tcgaagacca gatcaaagaa ggactttcct
    35281 atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc actagaattg ctagaacaag
    35341 tagggattcc taatcctgcg agagttggac gccaatatcc ccatgaacta tcaggcggta
    35401 tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc gtcattgcag
    35461 atgaaccgac gacagcccta gatgtaacca tccaagcaca gattcttgat ttactgaaag
    35521 accttcagga agaaacaaaa acagggatca tcttgatcac tcatgactta ggagtcgtag
    35581 cagaaatggc tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa gtagcaggtg
    35641 taaaagaact gtttgatcat cctaagcatc cttatacacg ctctttactg aactctatac
    35701 cacaagaagg aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt ccttctctga
    35761 aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat tccatggata ccagcaagcg
    35821 atcatgaaga agagcctatc cttcatgaag tcgcaccgaa ccacttggta cgttgctcct
    35881 gctataagca tttccacttt agagacgaaa aaggagaggt gtagatgaca gaattaatca
    35941 caatcaaaga cctgaaagtt cattacccta ttcgcagtgg attcttcaat cgagtaacgg
    36001 atcatgtata tgctgtagat ggtgtggatt ttattatcga aaaaggaaag acatatggtt
    36061 tagtgggaga gtctggctct ggaaaatcta ctacgggaaa agccgttgtg ggattagaaa
    36121 aggtaacagc cggacaaatc atgtatgaag gaaaagatgt aacgaagcga agcaaccgca
    36181 aaaaaatggg ttataacaag gatgtgcaga tgatcttcca agattcaatg tccagtttga
    36241 atcctaaaaa gcgagtgttg gatatcattg ctgaacccat ccgaaatttt gaacggttaa
    36301 gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga tatcgttggg atgccggaag
    36361 atgcgctata taaatatcct catgaatttt caggcggaca acgtcaacgt ctaggagtag
    36421 cccgtgctgt tgctaccaat ccaaaactga tcgtagccga tgaacctgta tctgctctgg
    36481 atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa gaatacggcc
    36541 tcagctattt attcatttca catgatttag gtgtagtaaa acacatgtgc gacaatatcg
    36601 caattatgta caaaggtcga tttgtagaaa tcggtacaag agaagacatt tataatgacc
    36661 cgcgacatat ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat gtagaaaata
    36721 gggaaagcca caaagaaaat cgacgaagag tagaacaaga gtatatccaa aaccaaaaag
    36781 aatactatga tgcgactggt cgagtctacg atttgcgtac gatcacaact acacacaaag
    36841 tggcgttgaa agatggaggt gcaagctaat gtggaaaacg attctgcgac gtgttttatt
    36901 gatgatcccg caagttatta ttttaagtgt tctgatcttc ttattagccc aagcgatgcc
    36961 gggggatcca tttaccggtt tgatcaatcc aaatcaggat ccggcagtga tcgaacagat
    37021 gcgacaagca gctggcttga atgacccatg gtatgtccag tacttccgtt ggatcggaaa
    37081 tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa cgctctgttg ctgacttgat
    37141 cggcgaacgg atcatcaaca ctttatatct atcgatcttg acagttgtcc ttacttattt
    37201 gattgctgtg cctctgggga tgcttgctgg ccggtatcaa aattcaatct tggataagat
    37261 tgtcgtaatc tacaacttct ttagttttgc tgttccttta tttatcttcg gcttgatcat
    37321 gctatttgtt tttggttatc gtttaggctg gttcccaaca agtggatcac aaacactagg
    37381 tttgcaaggt ggatttatca ctcaatggat agaccgtttg aaatatattc tgttaccatc
    37441 aattacccaa gcatttttgg cgactgccgt cacaatccaa tatctgcgaa gtgaagtaat
    37501 cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac cgacgaataa
    37561 agtattcagt cgacatattt tccgaaatgc agcgttgccg atggcttctc aaatggggta
    37621 tgaaatcact tcgttgattg ctggttctgt agttatcgaa aagatttttg gttatccggg
    37681 agtcggaaaa ctatttattg attccatcgg ccaacgggac tataccgtga tcacatcctt
    37741 agtattgatt ttaggaatcg cgacgctagt ggggacatta ctttcagata tcatcatgag
    37801 tatcgttgac ccgcgtattc ggattcaata aaaccaatca gaaaggagaa tggatcgatg
    37861 agtgaaaaaa acaatacagc agttcaagaa agtattcctc ctatggggat acgaatgatt
    37921 gctcgagaat ttaaaaaaga taaaattgct attttttcat taggtttgct agtggtttta
    37981 ttattagtga tctttatcgg tgcgatgctg accgatcaag acaaagtgat gtatgttagt
    38041 atcttcgaca aatacgctgc accaggaaca gtttcgacac aaggacaaaa gttcattctg
    38101 ggagcagatg aaggcggacg tgatgtatta ggacaattga tcataggtgc tagaaactct
    38161 gttacgatcg gatttgcgat cacgatcctt acttcgatca tcggggttgg attagggatc
    38221 atctctggct actacggagg gatcattgac aacatcttga tgcggatcgt tgattttatc
    38281 atgattctac cgatcatgct gatcattatc gtcttcgtat cgattatttc cagatacaat
    38341 gtctggtcct ttatattcat catgagtgca ttttattggg tagctaaagc ccgattattc
    38401 cgaagcaaga cattatctga agcaagaaga gattatgtca gtgcctcaaa aacaatggga
    38461 accaatgatt ttaaaatcat gttccgcgaa atcatgccga atctaagttc attaatcatt
    38521 actaatttga cgatgaactt tgcggcaaac atcggtatcg agacaacttt gactttctta
    38581 ggtttcggct tgccggcaaa tgtacctagt ttaggtacat tgatcggtta tgcaagtaac
    38641 ggagaagtct tagtgaatcg gcaatggatc tggttacccg catcaatcct tattttagtt
    38701 ttgatgttga gtatcaatta tgttggacaa gcattcaagc gttctgcaga tgcgcgccaa
    38761 cggttaggct agtatagaag cgaaggggga aataggaatg aagaaaacga tttttggatt
    38821 tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg ggcggaggta cagatacctc
    38881 tggaaacaac tctggaggca acggcaataa caaagaggaa ttaacttttc caattgcaac
    38941 aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg caacggacag
    39001 tcagtttaaa ggattgttcc agtgggagtt ttaccaagat tcatatgatg cagcttttat
    39061 ggctccttct catgaaccgc tatttgcaaa cgatgacgat ttcagaatca cagacgacgg
    39121 agctgcaacg cttgatctag atgaagatac gaaaaaagcg acaatcaaag taaaagacaa
    39181 cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat gtgattttcc cttacgaaat
    39241 cattgggaat aaagattaca ctggtatccg ttatgacgac actttccgta acatcgtagg
    39301 tatggaagaa tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa aaatagacga
    39361 caaaacagtt gaaatcgaat ataaagaagt aaacccaagc atgcttcaat cgggtggtgg
    39421 tatctggtca tatgcagcac caaaacatac gttagaaggt gtagcaatca aagatatgga
    39481 atcaagcgat caagtacgta aaaatccagt gacttttggc ccttattaca tgagtaatat
    39541 cgttgcagga gaatcagtag aatacttgcc aaacgagcac tactggaatg gtacgcctaa
    39601 attgaaaaag atcacgatga aatctgtacc gatggcaagt gcaactgaag cattgaactc
    39661 taaattatat gatatgattt tccaaatgcc aacagatact tacgatacct ataaagaaat
    39721 tgctggctat acaaacttag gccgtgaaca aacttcttat acttatctag gattcaagtt
    39781 aggtaaatgg aatgcagata caaagagtgt ggaatacaat ccagattcaa aaatggctga
    39841 taaatcatta cgtcaagcaa tgggctatgc attagataat aatgcagtag gtgaacgttt
    39901 ctatgcaggt ttgagaagca atgcgacttc attgattcct ccagtattcc agtcatacca
    39961 tgatgatgag atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac tattagatga
    40021 tgctggctac aaagatgtgg atgacgatgg tttaagagaa gacaaagatg gaaataaact
    40081 aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg caaccattgg cagattacta
    40141 tatccaacaa tggaaagaaa ttggtttgga tgtccaatac acaacaggtc gtctgatcga
    40201 attcaattct ttctatgata aattagaaaa cgatgatcct gaaattgatg tttaccaagc
    40261 tgcttggaat acaggaactg atccaaatcc aagtggacta tggggaccaa atgctgcgtt
    40321 caactacact cgttatgaat cagaagaaaa tacaaaattg atcaacgata tcagttcaag
    40381 caaagcattt gatgaagagt accaaaaaga agcatatcaa aaatggcaac aatatgcatt
    40441 tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa gtattgccag tcaacgaccg
    40501 tgtcaaagac tgggattggt cttatgatgt accaaaccca tgggcaaatg tttctgttac
    40561 agcagattca cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc ctttttcggt
    40621 tttttgatat ttttctgttt atttctctaa tattcaggtc aaggcgctga tttcatgact
    40681 tctccaataa acggtgttca aagtccagtt tctcggtatt aagtcaaatt tatgcaaaaa
    40741 tatggaaagc cattttcaca aaattttttc ttcatactcg aaactaaacg aatttttcac
    40801 aacttctcgt caatcactag gattattaaa gctttactta ccttttgacc gaaccatttc
    40861 aaatttgaca aaaaaccagt ataataagga agtatcgaaa aaatatccat aaaaggaatg
    40921 tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa actataacat cgtttttcac
    40981 gatctaaatt tgcttgaaca agcttttacc cattcatcct atgtgaatga gcatcgcaat
    41041 ctgcaattat cggataatga gcgacttgaa tttttaggag atgccgtttt agaactgatg
    41101 gtttctcagt atttattccg tttatatcct catttgccag aaggaaaatt gacaaaaaca
    41161 cgagctgcga tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg tcactttgat
    41221 caatatgttt tgttgggcaa gggagaagag aattcaggag gacgtacgcg accagcattg
    41281 ctttgcgacc tattcgaagc ttttttaggc gcgttatatc ttgaccaggg attcgacacg
    41341 acccatgcat tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc tttttcacat
    41401 gagatggatc ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga tgtatcaatc
    41461 gaataccgat tgatcaatga agaaggtcca gctcatgaac gagtattttg gatcgaagtt
    41521 tatgtagatg atcaattgat tggcactgga caagggaaat cgaaaaaatt agcagaacaa
    41581 gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag tgagggaaag ctttgtattt
    41641 gaaacgaatt gaaatagccg gttttaaatc atttgcagac aagacagtca ttgattttga
    41701 aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga aaaagcaata ttacagaggc
    41761 tatccgctgg gtattaggag aacaatctgc taaaagtctg cgaggcggaa aaatgccgga
    41821 tatcatcttt gccggttccg atacccgtaa gcagctgaat attgcagaag tcacagtgat
    41881 tttagacaat agtgatcatt atctgccatt ggagtacaat gagatcagtg taacccgccg
    41941 ttatcgacgg actggggaaa gcgagttttt catcaataaa caaagctgca gattaaaaga
    42001 tatccaagaa ttgttcttag attctggatt aggaaaagaa tcgttctcaa tcatttctca
    42061 aggaaaagta gaagcgattt tcagtagtaa accggaagac cgtcgtggga tctttgaaga
    42121 agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga agctttttga
    42181 aacagaagac aacctcagcc gcgtccaaga catcatccac gaattggaag aacagttaac
    42241 tcctttggcc gcccagagtg aggcagcaaa ggagttttta cggctaaaag aaacactgac
    42301 tcaaaccgat gtctctttga tggttgctga aatcaagaca gcaaaaaaag attgggacaa
    42361 caaacaagca cagcttgcga aattcaatct agaattaggc aaactatctg agtcgatcca
    42421 agaacaagaa tccattttgg cgaagcagcg taaagaaaat gcgcaagccg atcgtctgat
    42481 cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa ctgaagcaga cagaaggaca
    42541 aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag aatatcaaac
    42601 ttcattagca gaagcacaaa aaaaggttaa acattttgaa aagcttcaag aaagcttgat
    42661 gaaagcagca gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc tcataaaaac
    42721 acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa ttattggcag aattaagaga
    42781 tcaatatgtt gatttgatgc aagaacaagc agctgtagga aacgaactga agtatctaga
    42841 acgtcaatat atacaagaaa cagcaaaaag caagcagacg ttagccaagc aatcggaagt
    42901 agaagcatca gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa agcaggcgca
    42961 gctaaaatca tctttaactg aaacaaaaga gaagttagaa atgatccaac aaaacgggaa
    43021 aaagttccaa gaagcattag caaaagaaca gcctaaaatg tatcaattaa tgaatcaagt
    43081 ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc caagaaaact attttggttt
    43141 ttaccaaggg gttcgattgg tcttgcagca taaacagcag ctttcgggta ttgtcggagc
    43201 agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta gctatcgaaa cagctttagg
    43261 tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag ccatcacgta
    43321 tttgaaacag caaagaggag gaagagcaac attccttcca ctgacgacaa tcaaaccaag
    43381 acaattaccg gctcatatat tgacacaagc agcagctgta gaagggttta tagggattgc
    43441 cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc tgctaggaac
    43501 gatcctttta gccaaagact taactagtgc caatgcaatc gcgcaaacga tccgctatca
    43561 atatcgagtg gtttccttag aaggtgatgt gatgaatgcc ggaggatcca tgacaggtgg
    43621 agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat tgaaacagtt
    43681 gactagtgaa tttgaagaag cagataaaca actccaagca caagaaaaaa aagttcagga
    43741 gctgcaacaa gaaacagctc gtttagccga agaacaagaa gttttgcgta cacgaggcga
    43801 acaattacgt tttgaagaac aagaagcgac aaatcagcta caaaacatca taaatgaact
    43861 tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag acacgtgaac tgcagcagtt
    43921 tatcgaagat tacgaaaagc aaacgaacga attgaaagac aaacaaacag atcttgaatc
    43981 gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg accagatgga
    44041 agccagaaga gcacaagtac aaagtcaaaa agcacaagaa caagcagatc ttgctgtctt
    44101 gaaagagcag tttaatcatc tgcagatcca gctccgcggt gcccgagtcc aaaaagcaga
    44161 ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg acattgacag cagacttttc
    44221 tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt aacgaattat ctgctcagcg
    44281 agaaacatta aaagccgagt tggcaaaagc caaagagcag cgagatagaa ggcaaaagga
    44341 gatcgataag cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag cacgactgtc
    44401 ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa atgattttag ataatcattt
    44461 gacctatctg cagtcagaat atcagctgac atttgagaaa gcttcccaag actatcaaga
    44521 aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac agattgaaaa
    44581 actgggacca gtaaacttaa attcaatcga acaatacgaa caagtcagtg aaagacatac
    44641 attcttagca acacagcgag atgatctgtt agcagcgaaa aaccaattgt ttgaaacgat
    44701 ggatgagatg gatgatgaag tgcggacaag attcaaagaa gtatttgaag ctatccgtca
    44761 agaattcaaa attgtctttc caaatatgtt tggcggcggt aaagctgaat tagtactgac
    44821 ggaaccaact gatttgctga aaacaggtat tgagattgaa gtacaacctc ctggcaaaaa
    44881 actgcaaagt ctaagtttgc tttctggagg agaacgagca cttacagcca tcgcgttgct
    44941 cttttccatt atccgtgtct gtcctgttcc gttttgtatc ttagatgagg tagaagcagc
    45001 gttagatgaa gccaacgtga aacgttttgg tcgttacttg agcgatttcc aagatgacac
    45061 acaatttatc gtagtcacac accgcaaagg aacgatggtc gcagcagatg tactgtacgg
    45121 agtgacgatg caggaatccg gtgtatcaaa aatcgtttct gtacggatgg aagatatcaa
    45181 tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa attagtagca
    45241 atcgatttgg acggtacatt attaaatgaa caaaagctga tttcagatga gaataaacaa
    45301 gcattagcac aagcaaaaca gcaaggggtc aagatagttc tatgtacagg acgcccctta
    45361 gctgcgatgg cgcattatct gcaagaatta ggactggtag atgaaggtga ctacagcatc
    45421 actttcaacg gcggtttggt acaaaaaaat gatactggtg aaatcatgga aaagaaagtg
    45481 atggaagtat cagatatcca acgcttatac aaactggctc agcaattgga tctgccacta
    45541 gatgttttgt ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa gcaatccttg
    45601 tacaatgtgt tgaataagct gctgcaattc caaccagcgg atataagcga tattacagat
    45661 gaatttgtgc ttaataaggc agtaatcgct tatgaccaag aagaattaga tcctaaaatc
    45721 aaagaaatac cagcagaatt ccatgaaatg tatgagatca tcaaaacaag aagtatgctt
    45781 ttggaattta tgccaaaagg tgttacaaaa gcttatggta tttctttatt ggcaaaagat
    45841 cttggtttga aacaagaaga aatcatggcg atcggtgatg aggaaaatga tttaccgatg
    45901 atccaatatg ctggactagg cgtagcgatg gagaatgctg tgcctttcgt caaagaagca
    45961 gcagatttcg tgacagccag caatgaagaa aatggcgtag ccaaagtgat ccaaaaattt
    46021 gtattagagt agggggagtc aaatgggatt ttttgataaa atcaagcgtg cttttactgg
    46081 cgaagagaaa acagaagaag ttgaagtaca ggagaagtac gacaagggtt tagaaaagac
    46141 gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc aattttcgaa cagtcgatga
    46201 agactttttt gaagaattag aagaaacact tatcggtgca gatgtaggtt ttgaaacagc
    46261 gatacgaatt accgaagcat tacgtcaaga agtgaaactc agaaatgcga aaaaatcggc
    46321 agaagtccaa aatgcgatca tcgagaaact tgtggatctc tatgaagcgg aaggaatcaa
    46381 tgaagtaaat gaactaaata tccaagaaga cggactaaca gttattttgt ttgtcggtgt
    46441 taatggtgta gggaaaacaa caagtattgg taaacttgcc catcagttca aaaacgaagg
    46501 taaaaaagta ttgatggcag cagcagatac cttccgcgct ggagcaatcg accagttagt
    46561 tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag gcgatccagc
    46621 agctgtcgta ttcgatgctt tggaaagagc aaagacagag caggcagatg ttttactagt
    46681 tgatactgca ggccgactgc aaaataaagt gaacttgatg aacgaattag aaaaaataaa
    46741 acgagtaatc cagcgggaat taccagaagc accccaagaa acacttcttg tattagatgc
    46801 aaccactggt caaaacgcga tggttcaagc aaaacaattc aaagaaacaa cagatgtgac
    46861 cggtcttgtt ttgacaaaat tagatggtac agcaaagggc ggtatcgtac ttgctatccg
    46921 taatgaattg catttaccag tgaaattggt cggtcttggt gaaggaatcg atgacttgga
    46981 gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa aagaagaata
    47041 acccatgtct aaaaagcagg agctgtgaca aaagttgcag ctccttgttt gaatgattgt
    47101 ttttaacata gctgtgtttt tgatgccttc atttttatac ttatttgtaa gcggttctca
    47161 aacaatttcg ttctttttct tagacttttt gattataatt agattcatat gaattggagg
    47221 tcacttaatg atgaatttta agcaattatt attgcatgtc aatgcgcgcc cttttatcga
    47281 ccaagctcga tttggtatag aacgggaagg acagcgggtt gatcttgcag gaaatctagc
    47341 aaaaaccgat catccagcaa tctttggcga tcgatcctat cacccctata tccaaacaga
    47401 ttttagcgaa acacaaacag agatgatcac ccctgttacc gattctattc ccgaattatt
    47461 tcagtatctc gctgctgttt atgatgtgac tgctcgttct ataccgaaag aagagatgat
    47521 ctggccatta agtatgccac ctgccttacc ggaaaaagac gaagagatca ttattgcaaa
    47581 attaaaaaat ttcgaagatg tcttgtatcg acgttattta gcaaaagaat acgggaagcg
    47641 aaaacagatg gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt tgctaagaac
    47701 attgttcagc catcaggaag aatttcaaga tttttccgaa ttcaaaacag aactttattt
    47761 gaaaacagcg agaaacttta tgcgctatcg ctggatgatc acttatttat tcggtgcttc
    47821 accaatgagt gagaagaact actttttaga tgaatcacat ccgcaagaac ctgttcgcag
    47881 tattcgcaac agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt cctatgcttc
    47941 tatgaaacag tatttagcag atatcgagcg aatgatcgaa gaagggaaac tttcggagga
    48001 gaaagaattt tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag atttagcaac
    48061 aacaggtgtt cgttatattg aattgcggaa tatcgatttg aatccttatg caagattggg
    48121 tatcaatcca gagcaagttc ggttcttaca attattcctg atgtacatgt tatggacaga
    48181 agaaaaagaa gactgtgacc aatgggtagc agaaggtacg actcgaaata acaaagtagc
    48241 actagaacaa ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa ttcttgaagg
    48301 aatgaagcaa atgctggttg aattggattg gttggattct ctttatttag tagaggaagc
    48361 attgactcaa atggatcatc ctgaacaaac gttagcagcg aaactctatc aagaagcaca
    48421 gctatcgagt cagcaagaag ttgctgtggc attgggacat caatattata aagaaagcca
    48481 tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa ctttctactc agatatttat
    48541 gttcgatgcc atccaaaaag gtgtccaagt caaagtatta gatgagtcgg atcaattttt
    48601 gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat gcaaacatga caagtaagga
    48661 cagctatatc gtcccactta tcatggaaaa caaaacagta acgaaaaaag tcttaaaaga
    48721 agctgggttc cgggtaccag gaggcgcaga attttcatcc atggaagaag cggtaaaagc
    48781 ttatccgaga tttgccgagc aggcatttgt gatcaaacct aaatcaacca attacgggtt
    48841 aggtatcacg attttcaagg aaggagctag tctggaagat tatcaagcag gtttagccat
    48901 tgcttttcgt gaagacagtt cggttttagt agaagaattt atgccgggaa cggaatatcg
    48961 tttctttgtg atcgacggag aagtccaagc catcatgttg cgagtccctg ctaatgttat
    49021 tggtgacagt atccgaacag tgaaagaact tgttgaagaa aaaaatagtg accctttgcg
    49081 tggaacgaat catcgtgcac ctttagaatt gatccaatta ggtgaattag aacaattgat
    49141 gttgaaagaa caagggttga cgatcgaatc agttccccaa gccaatcaaa ttgtttatct
    49201 aagggagaac tcaaatatca gtacaggtgg tgattcgatc gatatgactg atgaattttc
    49261 tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta agattagtgg
    49321 tatcgatttg attataccag ataaagaaat cgatcctaca actgataaaa aagcatatgg
    49381 gatcatcgaa gcaaatttca atccagccat gcatatgcat gtttatcctt ttgcaggaaa
    49441 aggcagaaga ttgacgatga atgtattgaa attgttatat cctgaagtat tttaaaagaa
    49501 aaagcttttg acagattgtc ctatttgatg tcaatctgat caaaagcttt ttttgcatgt
    49561 aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt tttctttttt
    49621 ggtttttctt tttcagctgg cgaagagttt tcttctgggt attttttttc gatatcaatc
    49681 acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc aggatttgca
    49741 gtgacgtaat cgttgactac agtgaaacgg ctattgtttt tcgttgctag ctggatatat
    49801 tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagcatc tggatgtttt
    49861 ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc aacagtcata
    49921 ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc agggttcact
    49981 aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg tgccataaaa
    50041 aataccctcc ttcttctctt tgtctttctt acgattatag catattccca ataataccta
    50101 aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag aatgaaaatc
    50161 attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat tattagttat
    50221 tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag aactgctcca
    50281 gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa cgatttgttt
    50341 gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt tgacccaaaa
    50401 taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag gtattttagt
    50461 ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag cattttgctt
    50521 tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttcctcc agaaaaatca
    50581 attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag ttgctacttt
    50641 ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag ggaattttgc
    50701 gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt tgctcttggt
    50761 ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg cagtaaatct
    50821 gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat tggctagtgc
    50881 agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc cgaatcttgt
    50941 ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt tagcaggtag
    51001 tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt atgaaatacc
    51061 agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc tgataagggg
    51121 acgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt cttgttttat
    51181 ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt gcatgtgcat
    51241 tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc agtgccacaa
    51301 tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg ggacttgagt
    51361 cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg acgatgcttc
    51421 agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg ctgagtctat
    51481 tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta ctgatgtcag
    51541 ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc atcatggatc
    51601 cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat atccagagtc
    51661 agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta tttcgatatc
    51721 gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc gggatagaca
    51781 cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg acatcaacgg
    51841 ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt tatcgtgtct
    51901 tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct atgctgtttc
    51961 tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca gtgagtgtcg
    52021 tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa aggaagagta
    52081 tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag tggttaaaga
    52141 tgtacagtta acgattcctg aaaagaaatt gactgctttt atcggtccaa atggtgctgg
    52201 aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag gtgaaatcta
    52261 tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa aattatcgat
    52321 cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag tagaatttgg
    52381 acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg ttcaggaatc
    52441 actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc tttcaggcgg
    52501 tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt atattttact
    52561 ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc agattttaaa
    52621 aaatttagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata tcaactttgc
    52681 tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga cagttattta cgcaaggaac
    52741 gacccaagaa atgataaaaa aagatgtatt ggatgaatta tacgaaatgg acatacggat
    52801 ttgtgagatt gaaggccgca gattctgtct ttatttctaa aactaacatt aggagagaag
    52861 agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta gcagcatgtg
    52921 gaaatagcaa caacaccagt caggcagaca caaaaaaaag cagcacacaa actgaaacta
    52981 cattgacgat aacagatagt aatggagacc aaatcgaggt gccaaataat ccagaaaaag
    53041 tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt ggagataagg
    53101 tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa aaagtagaat
    53161 cagcaggtgg aataaaagaa ccagacttag aaaaaattaa tcagcttcag ccagatctaa
    53221 tcatcatttc tggccgacaa agagattttc aaaaagatct aagtcaaatt gctccgacca
    53281 ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat gtcactgctt
    53341 taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa ttatctagtg
    53401 caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta gtcacacttg
    53461 tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc atccatgatt
    53521 tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt caaagtgttt
    53581 cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac agaacaaaag
    53641 ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta gcacaaacga
    53701 atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat ttaagcgggg
    53761 gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt aaatagcata
    53821 aataacagag gattaaactc tcatactatg taacaataat gtttcttaca ttcttaaata
    53881 gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta ttgatgattt
    53941 tacggatggt ttttatttat ttgagaaatc ttttttcaac gtgaattcat tatctacatc
    54001 atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt ttttcagcat
    54061 caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat aaaacttaaa
    54121 tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc tgctgcttct
    54181 cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca gcgttcttat
    54241 tactcaaagc aaatcgcagt tgtcacaatc tcttctaaac gatgttccat ctgctgcttc
    54301 tcggcaataa gcccactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta
    54361 ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa tcagctgacc
    54421 ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg tctcagcaag
    54481 cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct tactccaccg
    54541 ttttaataat tgatagaaat aactctcttc tgctttgatt ttatactcgc ttaatatcaa
    54601 tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg ctttggtttt
    54661 atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat tttgtgtttc
    54721 ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa agaagtcttt
    54781 tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa aagtaattct
    54841 ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta tttaagaaag
    54901 gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca agaaactgaa
    54961 cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa cgaatgactt
    55021 actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct gatgaataaa
    55081 gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa agaagcacaa
    55141 gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat cgaaaacggt
    55201 gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt tcgattgatc
    55261 attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc tgatacacca
    55321 gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc atccacactt
    55381 gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt gagaaaaggc
    55441 aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc gatcatcagc
    55501 gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct ttggcaagtt
    55561 ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa taaagtagac
    55621 cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac atttcatttc
    55681 ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt tccagaccca
    55741 cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt agggatgaat
    55801 ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt acgaatgaag
    55861 cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga ggtcaatgca
    55921 gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg
    55981 attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc tgctccttat
    56041 gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga attacttgac
    56101 cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt tgaagataaa
    56161 tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga atggcagctg
    56221 acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat gaaattacgt
    56281 gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca tttgcaagag
    56341 gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa tgtgtaaaag
    56401 aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat cgtggatatg
    56461 ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat ttgtaactcg
    56521 cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa aaaaaatata
    56581 atatacattt tttatttaat tataattaat attgattgaa gtctaggcag tcaagtcaca
    56641 ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag ctgtatttct
    56701 agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct gataatcatt
    56761 tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt cataagtatc
    56821 gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt aaataaagaa
    56881 agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat gttattcagc
    56941 atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga atataacaca
    57001 gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt tgatccagag
    57061 aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg aacaggaggg
    57121 gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat ttctactgcg
    57181 gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa accgacatat
    57241 gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt ctctcaacca
    57301 gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa atttacgaaa
    57361 gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa ttctgaatta
    57421 acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg aacaggtgtg
    57481 gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc ggtacagctt
    57541 tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt agtttggaca
    57601 ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata aatacgtagt
    57661 catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa atttgtttgg
    57721 atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt gaaaaagagg
    57781 aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat catagggtac
    57841 tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag caaactcact
    57901 tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga ggatttaagt
    57961 atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga caaacaagga
    58021 ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat tatttctatc
    58081 gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa tgggaagtta
    58141 ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa taagaagatt
    58201 agtttactct cggattttac atttaaatct tatttatact taggaaatca acgagataga
    58261 gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag taatcccagt
    58321 caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc tggccaacct
    58381 tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg ttcttctgat
    58441 cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc ttttgtcacg
    58501 cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt agctcctaca
    58561 tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga gcaaagaagt
    58621 cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga tctcattagg
    58681 cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac tttttttcaa
    58741 gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc tgatgtaaaa
    58801 gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa gataactatc
    58861 agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca gactagtatc
    58921 gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa tcaaattgat
    58981 gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga ttattttatt
    59041 gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat tcctgaaaaa
    59101 gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca atcgaatgaa
    59161 ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta taaagataaa
    59221 gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg agagaagtat
    59281 aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc gaatacagaa
    59341 ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg caaactttat
    59401 ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag tagaccatta
    59461 agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc ttttggaagt
    59521 tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga gcttccagaa
    59581 gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc tgttacgatt
    59641 ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc tacagctcat
    59701 cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt tgaggggaaa
    59761 ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa tataacaaaa
    59821 ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca gagacaagat
    59881 tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga ccaaaacaac
    59941 aattatttat aacactccta gttattttag gggcactttt taacaatata tcagtgtcag
    60001 ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc gacaagagaa
    60061 aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa gcggaattga
    60121 gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca accaccaatt
    60181 caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc ctgatctttg
    60241 atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa agtgtccaaa
    60301 ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg gctggtggga
    60361 tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat caaagcttat
    60421 cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca ctgaacgaag
    60481 ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca agaaatgtca
    60541 tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt attgaaacaa
    60601 aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa ggattgcaga
    60661 ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg ctaaccccag
    60721 gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga aaattttcga
    60781 gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa aaagatttta
    60841 caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt aaaaaagaca
    60901 ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta ttatgcatac
    60961 ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat aacaaatagt
    61021 aaatttttat tattgtagga tggttgggct ctttttataa
//
LOCUS       DAFEHS010000008        59542 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFEHS010000008
VERSION     DAFEHS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.008"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(298..1608)
                     /locus_tag="LP327_000544"
     CDS             complement(298..1608)
                     /locus_tag="LP327_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL1724689.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1620..3038)
                     /locus_tag="LP327_000545"
     CDS             complement(1620..3038)
                     /locus_tag="LP327_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL1724690.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3278..4846)
                     /locus_tag="LP327_000546"
     CDS             complement(3278..4846)
                     /locus_tag="LP327_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL1724691.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4850..5260)
                     /locus_tag="LP327_000547"
     CDS             complement(4850..5260)
                     /locus_tag="LP327_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL1724692.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5257..6180)
                     /locus_tag="LP327_000548"
     CDS             complement(5257..6180)
                     /locus_tag="LP327_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBL1724693.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6177..7055)
                     /locus_tag="LP327_000549"
     CDS             complement(6177..7055)
                     /locus_tag="LP327_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBL1724694.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7042..8379)
                     /locus_tag="LP327_000550"
     CDS             complement(7042..8379)
                     /locus_tag="LP327_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL1724695.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8382..9485)
                     /locus_tag="LP327_000551"
     CDS             complement(8382..9485)
                     /locus_tag="LP327_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL1724696.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9559..11067)
                     /locus_tag="LP327_000552"
     CDS             complement(9559..11067)
                     /locus_tag="LP327_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL1724697.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11033..11725)
                     /locus_tag="LP327_000553"
     CDS             complement(11033..11725)
                     /locus_tag="LP327_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL1724698.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11747..12721)
                     /locus_tag="LP327_000554"
     CDS             complement(11747..12721)
                     /locus_tag="LP327_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1724699.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13021..13893
                     /locus_tag="LP327_000555"
     CDS             13021..13893
                     /locus_tag="LP327_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL1724700.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14351..16549)
                     /locus_tag="LP327_000556"
     CDS             complement(14351..16549)
                     /locus_tag="LP327_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1724701.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16781..17773
                     /locus_tag="LP327_000557"
     CDS             16781..17773
                     /locus_tag="LP327_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1724702.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17833..19254
                     /locus_tag="LP327_000558"
     CDS             17833..19254
                     /locus_tag="LP327_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBL1724703.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19247..20746
                     /locus_tag="LP327_000559"
     CDS             19247..20746
                     /locus_tag="LP327_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBL1724704.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20785..22179
                     /gene="uxaC"
                     /locus_tag="LP327_000560"
     CDS             20785..22179
                     /gene="uxaC"
                     /locus_tag="LP327_000560"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL1724705.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22338..23021
                     /locus_tag="LP327_000561"
     CDS             22338..23021
                     /locus_tag="LP327_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBL1724706.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23023..23859
                     /locus_tag="LP327_000562"
     CDS             23023..23859
                     /locus_tag="LP327_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL1724707.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23870..25624
                     /locus_tag="LP327_000563"
     CDS             23870..25624
                     /locus_tag="LP327_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1724708.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25900..26241)
                     /locus_tag="LP327_000564"
     CDS             complement(25900..26241)
                     /locus_tag="LP327_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL1724709.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26309..28480)
                     /locus_tag="LP327_000565"
     CDS             complement(26309..28480)
                     /locus_tag="LP327_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL1724710.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28649..29548
                     /locus_tag="LP327_000566"
     CDS             28649..29548
                     /locus_tag="LP327_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL1724711.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29619..30476)
                     /locus_tag="LP327_000567"
     CDS             complement(29619..30476)
                     /locus_tag="LP327_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL1724712.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30461..33163)
                     /locus_tag="LP327_000568"
     CDS             complement(30461..33163)
                     /locus_tag="LP327_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL1724713.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33176..34465)
                     /locus_tag="LP327_000569"
     CDS             complement(33176..34465)
                     /locus_tag="LP327_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBL1724714.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34615..35661
                     /locus_tag="LP327_000570"
     CDS             34615..35661
                     /locus_tag="LP327_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1724715.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35663..37816
                     /locus_tag="LP327_000571"
     CDS             35663..37816
                     /locus_tag="LP327_000571"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBL1724716.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38289..39740)
                     /locus_tag="LP327_000572"
     CDS             complement(38289..39740)
                     /locus_tag="LP327_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1724717.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39737..41461)
                     /locus_tag="LP327_000573"
     CDS             complement(39737..41461)
                     /locus_tag="LP327_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL1724718.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41454..42068)
                     /locus_tag="LP327_000574"
     CDS             complement(41454..42068)
                     /locus_tag="LP327_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL1724719.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42413..43870)
                     /locus_tag="LP327_000575"
     CDS             complement(42413..43870)
                     /locus_tag="LP327_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL1724720.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(43911..44831)
                     /locus_tag="LP327_000576"
     CDS             complement(43911..44831)
                     /locus_tag="LP327_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL1724721.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44843..45790)
                     /locus_tag="LP327_000577"
     CDS             complement(44843..45790)
                     /locus_tag="LP327_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1724722.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46269..46988)
                     /locus_tag="LP327_000578"
     CDS             complement(46269..46988)
                     /locus_tag="LP327_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL1724723.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47037..48683)
                     /locus_tag="LP327_000579"
     CDS             complement(47037..48683)
                     /locus_tag="LP327_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL1724724.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            48862..49452
                     /locus_tag="LP327_000580"
     CDS             48862..49452
                     /locus_tag="LP327_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL1724725.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49544..51049
                     /locus_tag="LP327_000581"
     CDS             49544..51049
                     /locus_tag="LP327_000581"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBL1724726.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51133..52485)
                     /gene="celB"
                     /locus_tag="LP327_000582"
     CDS             complement(51133..52485)
                     /gene="celB"
                     /locus_tag="LP327_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL1724727.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52485..53003)
                     /locus_tag="LP327_000583"
     CDS             complement(52485..53003)
                     /locus_tag="LP327_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724728.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53019..53345)
                     /locus_tag="LP327_000584"
     CDS             complement(53019..53345)
                     /locus_tag="LP327_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBL1724729.1"
                     /translation="MAEKINSETLQVPSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53358..55337)
                     /locus_tag="LP327_000585"
     CDS             complement(53358..55337)
                     /locus_tag="LP327_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL1724730.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55358..55672)
                     /locus_tag="LP327_000586"
     CDS             complement(55358..55672)
                     /locus_tag="LP327_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBL1724731.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55840..56133
                     /locus_tag="LP327_000587"
     CDS             55840..56133
                     /locus_tag="LP327_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBL1724732.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56211..57323)
                     /locus_tag="LP327_000588"
     CDS             complement(56211..57323)
                     /locus_tag="LP327_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL1724733.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57476..57700
                     /locus_tag="LP327_000589"
     CDS             57476..57700
                     /locus_tag="LP327_000589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724734.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            57710..57889
                     /locus_tag="LP327_000590"
     CDS             57710..57889
                     /locus_tag="LP327_000590"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724735.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58083..58253
                     /locus_tag="LP327_000591"
     CDS             58083..58253
                     /locus_tag="LP327_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL1724736.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58677..59537)
                     /locus_tag="LP327_000592"
     CDS             complement(58677..59537)
                     /locus_tag="LP327_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL1724737.1"
                     /translation="MDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKA
                     NRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIE
                     KEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFI
                     SIKFRKKRNI"
BASE COUNT        17798 a        12175 c        10414 g        19155 t
ORIGIN      
        1 cttttttaaa agtttttttc gatttcgttt ttcgaaatct ttcagcaact catttatcat
       61 aacttatcgt ttgttatttg tcaagaactt ttttgaagtt tttttcgata gaatcaagct
      121 tcgcaagtgg ttctttttaa cgacttagtt atcttatcac gtctaaaaac atgtgtcaac
      181 tcttttttgg aattttcata aaatgtttca atgaagcgcg acaacgttgt ttaatatatc
      241 attaaaaaaa ataaaggtca agctaaaata ttatttaagc ttgaccttat tataatttca
      301 acgattgtca aaagtattgt ttgcagctgt gttgccagtt tctatagata aatcacagct
      361 tttgaaacat aagttttgta ctgcttcgat tcgcccgaat tcttttgcag ttatttggca
      421 atgagaaaag cagacatctt ctataggctt ttcaggaaat gctactaaat caaaagctcg
      481 ggaaggaagt gagtaacctg gagaaaggca tgcctgtacg tttttgatgt gtatgtcttt
      541 gactttcgtc atttgctttg attcaggtat ttcttcagcc actgcttgcc aactttctgg
      601 catttgagca aggtcattgc tattttttct attgtattca ttatgccagt ccattatcca
      661 agaaaaagga aattgtacat caatcatatt cagattttct accctgatat tttcgatcac
      721 tccccctctt tcttttgaag atttcattcg aataccacag tctgactgaa aaaaatcgat
      781 atcgtgaata tatacgtcac taactccagc actaacttcg ctaccaatag ttacaccgta
      841 tcctgaacgg attttacatc tagcaacctc gattcttgat gattttcgat tgactcttgc
      901 accattgcca tctctgccag acttaatagc aatacaatca tcaccacagg ccaaatcaca
      961 ttcatagaca cgcacatttg ttgaagaatc aatatcaatc ccatctgtac ttggtccatc
     1021 attgttgcgg atgattactt tttctactaa tacatcgtta gaataagtga tttgcaagtt
     1081 ccaaaaacct gatttctgaa aaatcaaatc acgaacaaca acatgttctg catgatacag
     1141 taaacaagcg cgtggacgtt taatagcata atccgcaatc caacgaagtc ccttttgatc
     1201 gtattcagct cttgtcccgc ttttttgatc ttttccccaa tacagctccc accaatggtc
     1261 tccttgaccg tcgattattc cttttccttc tataagaata tctttcgccg agagaacatt
     1321 caaaatcgct gctggccatt tcatttctac gccggctact cgtgtatcga tttctggaaa
     1381 tgcttcgata tctgttctcc ccttgaggac tgcaccttct tcaaagataa gatgactctt
     1441 atcttttaaa aaaagggctc cgactaaata aatgcctttt ggaataaata caggtaagtg
     1501 ttctttcgca ccttgatcga ttgctttttg cagtattatt gtatcatctt gttgagtgtc
     1561 gtttgtttta aagttcaaag cattgataat cccttttttt gtccgcaata cgtttctccc
     1621 tatctttcat tgattttttt caattcttca ccaataaata cagtttttga acgattcagt
     1681 gggtaaccta ctagcaaaat gattgcggat acaaataagc agaatgctgg taacaagttt
     1741 gccatcgtat aaatcctttc ttgtactaaa actgattgga tggctccacc tgttgtagac
     1801 gattggtaac caatgagtgc cagcataaat ccgcctaccg ctcctgcaca tgcttgtcct
     1861 acttttctcg caaaagaatt tactccatat actgttccat cttctcttaa gcctgtctta
     1921 tattgatgat aatcaatcac gtcagtaatg aatgcccaga ccatcaaatt gaataaacca
     1981 gatcctaaag tagcgataaa taaaagaatc agatataccc atgcatttgt tatatgaagg
     2041 aaatacatca gcaagtacat acatgaagaa gcaagtaacg caaatacgct tgcttccttt
     2101 ttaccaaata attttgtcag ctttgtagca aatggtgcta aaactactac tgttccgtaa
     2161 gtaaataaaa gagcaacaga cattgcttgt ttattatgga agtaatcatt gaataaataa
     2221 gtcatattcg tcccagctaa aatctgattg atcacaatga aaatgtcaac aatgactaaa
     2281 ataatcagtg ctcgattagc taacaaacct tttaccaact gagaagcaga tactttttcg
     2341 tttttgacaa ctcttactct ttcagtagtc aatttacaag ttaacatata tgcgacaaat
     2401 gctattgcag cacatccgat tgaaatccaa aagaaatgat tccctgaaac gacttgttgt
     2461 ccactcttgt tatgggtata cattaaaatc ggaatgaaga atcctgtagt tgatccgcca
     2521 atcgcagaac caatgcttcg gaacgtggac aacgaggtgc ggtcattagg atttgagcta
     2581 attgctgctg ccattgctcc atacgggata ttgattgttg agagaaaaat cccataaaca
     2641 atgtatgaaa caaaaatata agcaatcttc accgtcattg gaagatgttg gacgaatggc
     2701 aaaaacaaaa tgacagtcac taagcaaaat ggatacttca tttttctgat ccaaggaata
     2761 aatcggccag tttctgttaa ctctgagaca tcacataatc ttccaaccat cacatcagca
     2821 aacgcatcca aaattctcga aatcaagaat aacgtaccta cgattgcccc tgaaacgcca
     2881 agaacattcg tataataaat catcaagaaa ctattgacta aacctaaaat aaaacagtta
     2941 cctagatcac cgaacatgta gcctatctta tcggaaatcc caaattcctt tttttctttt
     3001 tttattgtat ttgctgtaac agcattttgt gaaagcattt gaattcctcc tacctcttct
     3061 agtaaacgtt ttcaaaatta taactctttt tttcttgttt ttatattcac aatgttggct
     3121 ttttattgca ttatcttgac agataaaaaa caaaaaataa tctaaaaaca ttgatgcata
     3181 agattgataa ctttttttac ataacaatat atacaaacaa aaaaattcac taactagtga
     3241 aaataagtta gtgaacctca tttattaaag aattaaatta cagatgatct tttccatcat
     3301 acttgaaaaa ctcaaaatca gcataacttc ctcttttttt atttagatca tgcactgcta
     3361 tcccgataaa atttccagta aaccctcctg agagtataca catgtttttt tgcggaccta
     3421 ctttcttcca ttcttcagtt gatacgtctt tcaaatgaaa ctgtgctgtc tctttcttta
     3481 cagtgacttt caaaaaagtc ttcttttctg tgagtggaat cgtccaatct tctatttgtg
     3541 ctactccgtt ttctgattct atcatacgta ggcattttcc cttgtcctca ttccaagtaa
     3601 cataacaata aatataattg gattcattta agtacagtac cagtccagcc atctgattaa
     3661 aattattagg catgtattca aatgctgtct ccgcatcaaa agtaaaatcc gtttgccgaa
     3721 tagctaacaa atgatgttcg aaggtagatt gaagagattc accggaaatc agacgtaaaa
     3781 aacctggtct acattttgta tcacaccaat cagcatttgg caaaatgcgt ctggcattcc
     3841 agttcttctt tagctctcct tcaaatgtat ccataaactg atgatctttt tcttgttgta
     3901 cctcttcatt tgtctcaatc attatttctt ccatcggagt tgtctggcta tctttaagtc
     3961 ttaaccaatc gtcggacgtc caatacactt cttgaatagc ggtttctctt cccaaaatag
     4021 ccgcaccgtt taatggtctg gtacaaagat atgtcatgta ccatcttcct gacggggttt
     4081 gtactaagct cccatgtcct gtacattgca atgtagaagc aggtttgtca ctagctgtca
     4141 gcataggaaa accaggatca agctcgtaag gaccagtaat attctttgct cggcaaactg
     4201 taactgaatg tcctcttcct gtaccgcctt ctgccgtcat caaataataa tacccgttac
     4261 gaaaatacaa atgtggtgcc tccgttttcg ctaattctgt acctgcaaat attttataga
     4321 tgggaccatc aagcttcttc ttctctgcat catattcttg taatacgata ccgctggatt
     4381 tattcggtgt gtccagacga taatcccata gttcattgac aagccatttt tttcccgttt
     4441 caagatcatg gaacaaagaa ggatcaaagc cgttactatt taaataaatt ggttcggacc
     4501 aaggacctgt gatctcagat gcagtgatca aataattgtg gacatcttta aacggacggc
     4561 ctgcactttt gacatctgtg taaacaagat aaaacaatcc atcagcataa ctgatctgtg
     4621 gcgcccatat acttccattt gtaggatttc cttgcaaaga tacttgagtg gtcaaaatat
     4681 ccgttgtatg tgaccagttg actaaatttt tcgactcata tactcggatt cctggaagcc
     4741 attcaaaaga ggagacaata atgtaatacc gctctttcac tcttaatata ttaggatctg
     4801 gattgaatcc tcttaaaata ggatttttca ctttgatagt ctgcatgatt caggctcctt
     4861 ttctcattct tactcgatat aaactgaatg ccccagctgc gaaataaaaa aatgcaaaag
     4921 gaaacgcaat aaactgagtg ccaatcaaca atatgaatcc tgttataaaa tacaaaccag
     4981 tggcagttgt taagtatttt cctttagcag tcaaaaacaa tgctactgct aaaaaagcaa
     5041 gtgaaataaa caacacaacg gcaaacagac cagtcatgtc ctgtaataat ccaaatgtat
     5101 tttctaaaga caaagttgaa tgatctacag caagcgactg ataacttttt ttaaactctg
     5161 acgcagacat attacttaaa gtaactgcta acccgccaaa gaaaatcaaa gatagactgc
     5221 caccgaccat tcctagcaat agttcccatt ttcttttcat ttctgaaccc cctgcttttc
     5281 taaatgtatc cattcaggtc ttttgatcga tgtttcataa ttgttattac gttcagagaa
     5341 tttgacagtc tgttcatttt tcttgtcatc ccaattatcc caaccagttg ggtgaacatg
     5401 cccacctatc ttacacgctt caaataagac atttgcatac ggacgccacg gtcttcctaa
     5461 atagacgttt ctagtatcag gttctgcagt caaggtacag ctttcaaaac gataccctgt
     5521 tttttgattt ttcggcgtag atgctgctgt gatgtatcca ggatttttac tggctaagct
     5581 atggatatga cactgatgaa aaacggcatc tgccccacca aatatgaaat ccaccgttcc
     5641 ttcaatccag caatattcat aggtctgacg acaatactca ggattggcag aagccggtgt
     5701 acaaaaagga gtaccatctt tttgcaaatc aggcaacgga cccgtgcata aggtatcctg
     5761 ataaccagat agtcgacagt tttttaaaga tacgcgatct cctgaattga ataaagcaac
     5821 agcctgtcct accttttctc ctggacctgc tgtattttcg atgtgaagat tttctaatac
     5881 gacattcgat ccttctaaga aaaaagtagc acttcggaaa gtccctaact ggctaccgtc
     5941 aagatactcc tgttttgcaa aacgattacc ggagataact actgttcctg ctcccacaag
     6001 tttaaaattg gataatctgc tttctatata ttcatcatat cttccttctt ttactatcaa
     6061 gacctttgga tggctatccg gcaggcctgc caaataatcg attccttctt ggatagtttc
     6121 aaaatccgtc ggggtatcta tagcattctt tccaattatc agtttgtctt tcatcctcat
     6181 aaatgctccc ttatgaattg tttccaaata tttctcattt cttgtgtttc catatgaccg
     6241 cctgttaaag aaaaagaaga aaagttttct ggactaccca ttgattgata ctttttgtcc
     6301 agcccttttc tcagcttcag tacgccttcg tccaagcaca tcgtatcgtt ttttccaact
     6361 aagctaagac gagggcgcgg agcgattttt tcttggattg ctaacgttgt ataagctgtc
     6421 aatagatttg gcacatagta ataaaaccca tgatgatcta ggcggcgatg tagcaaaagt
     6481 atttcgatgt ccacctgtgc cgctagatca atacaaactt tgacacgaga atccaatgct
     6541 gtcacccacc agcttaataa gcctcccata gaaagaccaa tcgttgcgag ccgttgagta
     6601 tctgtgtctt cccttgtctc caaataatcg atcaagctga tcacatcata aatacgcatt
     6661 ccccataacg tttttcctgt taataaaaat tgtttgaaca actcgctttc agaaatacct
     6721 ccgcgttctt caaatcccca agcatcaatc gaccaaacag catatcccat tcctgtcagt
     6781 acttctgcaa aagatggaga agctaagtaa ggagcaccat ggatcaattc acttttcccg
     6841 actgaaaaat ctccgccgtg agaatgcagg taaaggaccg gcggaaacgg tccttctttt
     6901 tctggataag catagagggc tggtactgtt tccaattcat ttaagtacaa ttgatagtat
     6961 tcaagcataa aaccgttttg tttttctcgg tataatcgtt ttccactagg ggtatgtctg
     7021 ctagatggag cacctaaaaa atcattcaac tgcataagca aagtttttac gaacaaccag
     7081 ttcatccata ttttctgatt gatttgattg gaatttctca atactgattt ctttagtatt
     7141 ttctaataga aatgctggtc cctcgtgatt ttcaatcgac atctggttaa aggaaacctt
     7201 acccgcaaag ccaatataaa atccttgtct gctcatttcc tctattcctg tcatcatagc
     7261 aggaacaccg ggaactgcat cttgggccat cgagacatcg atattgtcaa atgtgatttc
     7321 ttgtatgaat tgttccgcta atccataaat aaaacctgca gaagcatgga cattccttgc
     7381 cgtgatattc gaaaaatgga tccttctgaa tgctggtgtc cgttcatcta tcggataagc
     7441 ctttttttcc catacatacg gttctttccc tcgcggacca cagaaataat aaagattcag
     7501 tataaatgga cacatcacat tatccatgac tatattggat actcgaatat cttcaacgat
     7561 cccgcctcga cctcgtctag atttcaatcg tattcctcga tcagtttctt ggaagataca
     7621 atttgaaatg gtaatattac gaatactacc actcatttca cttcctaata cgacaccgcc
     7681 atgtccatgg accatcgtgc aatttgtgat cgtgatattt tcgcaagcga tcctttcgta
     7741 cgtatcttca gtccctgcct tgattgcaat acagtcatct cccacgtcaa tatggcaatt
     7801 actaatacgc acatttttgc atgattctgg atcaattccg tctgtatttg gtgaatccgc
     7861 tgggttcaag atcgtcaagt tatcaaacgt tgcattactg caaagaatcg ggttgatcgt
     7921 ccaacttggt gactggatca atttaatatc tttcacagta attcgatgac agttatggaa
     7981 actcatcaat ttaggacgag gataagcgag attatctggt tcatttctaa aagtatgcca
     8041 ccattttttt ccatttccat ctaatgtacc gaaaccagtg acagagatgt tctctacatt
     8101 ttgagcatag atacaggaag cataaacttt tcgatggact ccttcccaac gtgaatgaac
     8161 aacaggataa tcttttggat cgtcagagaa tttcagaacc gctcctgcgc ttaaatgaag
     8221 ttcgacattg ctttttaaaa ataaagctcc tgttagaaat tcacctgctg gaacaactac
     8281 ggtcccgcct ccgtcacttg ctgcagcatc aatggcttgt tgtattgctt cagtattcaa
     8341 ttcatcaatt gatgcgccaa atgtcaaaat atcatacata tttataatgc ctccacttca
     8401 atcaatgctt gcaagaatgc accaacgcct ttttgatcat tacaaataat tggttcactg
     8461 atgtagtaag catatgaacc atctcgctga tctgctccgc ctaaccctgc tacttgacag
     8521 tttttattca agttcagcca gccttctttt gtttccagaa caaattcatc aagtaatcct
     8581 tgatgtgatt tcttcacttg tggaagccat tcttctttgt tcagcagtcc caaacgtatt
     8641 cctttagcca ttgcgtaaac aaacatacta ctgccggaag cttccagata atttcctttc
     8701 cgttttgttt ctgttgttac ttgataccat gtcccgctgt tttcttcttg atatggaagt
     8761 aatgcagcca gtgtcttttc tagaatttct ttcagcaaag aacaatctgt ttcttcatga
     8821 agtaattcat aggtatctgc tgcagccatc aaataccagc caatcgatcg gctccaaaaa
     8881 tgatgggata gacctgtttt tggatcagcc catggttgga ccttcttttc atcccatgca
     8941 tgaatcagca aatttgtttt tggatcgaat agatgtttat aactgatttc aaactgtctc
     9001 aaaacatctt ggtaatcttt ttcttcacta aaattctgga taaattcagc ataaaaagga
     9061 gaacccatat ataagccatc caaccaaatt tgataaggat aaatttcttt atgccaaaaa
     9121 gctccttcgg atgtgcgtgg atggtttgcc aactgctggg aaaggagttc tgctgctttt
     9181 ttatattttt cttcgcctgt ttctttgtac aataaaaaga aaagctttcc tgtatttaca
     9241 tggtcgatat tgtattcttc aacgctgtag ccacggatcg ttccatcttc ttgcacgaaa
     9301 tagtcgatat tgtcttttat gaaatcaaaa taaatttttt cttgggttcg ttcccaaagt
     9361 aattggaacc ctttaagtac gacgccatag tcataagacc attttccttt ataccacttt
     9421 tcttcatata agcgaggtgt tctttccatg atactttttg ctaatttctt tgaccagttt
     9481 tccattattt gctctccttt aaaaattaaa ataggatttg ggacaaatac caagaagtat
     9541 ttgtcccaat cgtctatatt actgtgccgc tgctttgtat gcttcctcgt actcttgtgt
     9601 gattcgatcg cctccttgtg ctttccagtc ttctacagct tttttgaatc catcttcgtc
     9661 aagctgtccc atgatgtatt gcaccgtagc atcaatgacg attttttcaa tttctgatcc
     9721 tacttcattg ttcgtcggtg aatccaatgg cacagaagga tccattacag caaattcttc
     9781 attttctttg atcaattgat tagagaattc tttttcaggg tctgtatctt tgaaagagaa
     9841 cgtcacttca ctaggacgag agcttgataa cggttgcacc tcttgttgcc aaagatccat
     9901 atcgataaat tcgatagaac catcatcatt caattcataa tgcgttcctt cgataccgcc
     9961 agtcatcaat ttatacacat cttcttctaa aagatcatca acaaattgaa gtaatttctt
    10021 caattgtgct tcgtctttta cttgagattt gggaaatgct agcaagccgc caataccatt
    10081 tccttctgac caaacatgat aaccgccgcc ttcaccatct gagatcttgt ttacaggcac
    10141 taattccata tcatcttgaa taccttgacc catcgtcttc agattcttga tatccaccat
    10201 tccagtgtag attccagttc ttccttgtgc aaattgttct tgctgatcag tcttttgggt
    10261 aactgcaaag tcttgtgcca aatagccatt ctcaaataga tctcttgaaa aattcaaagc
    10321 tttcatatac tcttctgtat caaattctgg agtgaatttg ccattatcgt ctacagccca
    10381 gccatttggt gctccaaaat aagaagtgaa aagtttaaag ctggtataac gaatatcact
    10441 aggcgcacga tcgccaaaac caactgtgtc gtccacacca tttccatcag gatcgttttc
    10501 tgtaaattgt ttagctactt cataaagctc atccatggta gttggaacat ctagtcctag
    10561 attatccaac cagtcttttc gaatgactaa gccagcacga gcgtagtctt tttgtatcgg
    10621 tacgccgtac aaggcaccgt cgatactcgc agcttcgatt ctatcttcag aaatttgact
    10681 taaattttta tagtctttta aatattctga tacatcccag aatacgcctg attttaacgc
    10741 attgcggaca gaagagtttt tcaacatcgc aatttgaagt gtaacgatat ctcctagttc
    10801 atcagaagcc aatgcagttg ttaatctttc ttcttttgaa gcatcaggaa tccagttgaa
    10861 tgtcagttcc gtatcggttt tttcttcgat caagttcaca atcgtgtctg taggcggtga
    10921 agcatgatgc aaaatattta accaagtgat tttagctttt ccatcttctg tctctgaaga
    10981 actgttactt gaactgctgc cgcatgcggc aagtgctaga attcctgcaa aactaagacc
    11041 taattttacg agcgttgctt ttttcataaa ttttctcctt cactgtttta ataatatttt
    11101 tgttttcttc tgttttttct gtatatgagt tcttccattc caaatggcaa aatatagaat
    11161 ttgctgtcca cgtgactgcg gaaaagaaga gactgccacc gataaaggtt aaaacacctg
    11221 gcattgtttg gatgatcatc agataaatcc ctaccatact taatatcaac actaatgagt
    11281 aatgaagatg tgagaatccg aataaaaatg ccattttcat tttgaaaaaa tatcctttcc
    11341 aatcataatg agccatgatt gggaaaatat agagtgctga gagcacatat atgaacgcag
    11401 caatatacaa agcgatccta agataccaat tgtgtatgaa agaaagatcc acaagtaaaa
    11461 caatcccgat ggcgccatat atccagctca tcagcaaggt ttcacgatag ttttctttaa
    11521 aatatgtgaa gtacgttttc gtcaaaggaa acgtttcttt tgagcgcagc cattttctaa
    11581 tcactgcaac catcgcatac gatgcaggac cgaaagtaaa aactcccaaa cctgcgatga
    11641 ctaaaccgcc ccacagaaaa ttcaaataaa ccaactgtaa aatacgattt agccttccgt
    11701 ttattttatc taatgactgc agcatatctt tccctctctt tctatcttag taaacgccgt
    11761 cttctcctag ctttttggca atcttattag ctaaaattac cataaacaat cctacgaccc
    11821 ctttaaacaa tcccattgct gtactgaaac ttaactgtcc attcttcaat ccagctgtat
    11881 aaatataggt gtcgaaaatt tctgcaacgc ttcgattcaa tgaattcaat aacaagtaca
    11941 tatgctcgaa ccctaagtct aatgtctgac cgatttttag aatcagcaag gtaatgatta
    12001 ctggacgaat tgccggcaat gtaacatgcc atgttttgcg taagcgtcct gctccatcca
    12061 tttctgcagc ttcatacaac tgtgtatcga ctgcagttat cgctgaaagg tagatgatcg
    12121 tagaccagcc caactctttc cagatcactt gtccgatata tactgtgcgt gtccattcag
    12181 gggatgttag aaaactgatt ttttcaaatc ccattgttgc aaggaattca ttcaataccc
    12241 ctccgtcaac attcaaaaat acataagtaa tcgaaacaat gattacccaa gacatgaagt
    12301 gagggatgta aatgatcgtt tgaatcccgt ttttcaatcg tttgttcgtt acttcattta
    12361 atagtaaaga caaaatgatt ggcatcggaa aaaacacgaa gatatttaat ccgaacaaga
    12421 tcaacgtatt tcttaataat gtaaagaatg tcggttctgt aaacaaacga atgaaatgtt
    12481 tgaaaccgac ccaaggactt cccattattc ctaaaaatgg ttgataatct tgaaaagcaa
    12541 taacgagtcc ccccatagga agatacctaa aaatcaagaa ataaaggact cccggcagaa
    12601 tcatcacata taaaaattta ttcgttttga ttcctgcaat tatttttttt cgtcttctga
    12661 gcttttcttc ttttttcgat agcgtcagtt gaaatggttc ttgtttcttt acactttcca
    12721 taagcccctc cttagttttg tgaatgtttg ctgcttatga cttcatgata gataactatc
    12781 tgaattacgt atataatcat ttttaccgta agtaaaaacg cttacatagc aatgattttt
    12841 gtgctataca aaacgataaa gtgtattttt tcacaaataa actacgcata gcaaaccttc
    12901 tatttttttc tatctgacac ttagcatagg aaatgattat atacgtaatc tttcaccgat
    12961 gataaattga acatatagaa acagaaacat ttaatttatt ttgaggagga gcctatctat
    13021 atgaaaaaga aagtctctat aggagaaaga agttttacac ttttcaacaa tacgtttcta
    13081 attttgctag ctttgatctg tatcgttcct tttttaaata tcatcgctac atcttttgct
    13141 tctacacaag aagtcgtcgc gaaaaagttt atcctttttc ctactacctt ttctttagac
    13201 gcgtatcgct acattctttc tacgccgacc atttttagag cacttgctgt ttcgattggt
    13261 gttacaggtg tcggaacgat cgtcagtatg tgtgcaactt cactgatggc ctacggtctt
    13321 tccagaaagt atttgtttgg tcgaggtttc gtcaactttc ttgttgtatt ttctatgttg
    13381 ttcagcgggg ggatgattcc aacgttctta gttgttcgtt ctctagggct ggtcaattct
    13441 tactgggcca tgatcctgcc tgtggctgtt aatgcaatga atatgatcat tatgagaaac
    13501 tttttccaag ccttgccgga tagtttggaa gaatcagcaa aaatggatgg ttgcacagat
    13561 tttggtgtat ttttcaaaat tatgctgcca ttagcgttac cgtctattgc tactatctcg
    13621 cttttttatg ctgttactta ctggaacact tatatgactg ctattttgta tatcaacgat
    13681 tcatctaaat ggccgataca gattctttta cgccaaatcg tcattgtatc aagtgggatg
    13741 caagcagaaa gtagtgccgt tgatgtgatt ccaccagcac aaacagttaa aatggctgtt
    13801 atcgttattg caacagtgcc tatgctgatt gtttatcctt ttgttcagaa atattttgtc
    13861 aaaggagcac tcgtaggttc tgtcaaaggg tgattatgta ctgaacgcaa aatggtagtt
    13921 gtgtcagaag tggtcagctt caagaaataa gaaggcattt acgaaaattg cttcttaaat
    13981 ttttgtgaaa tttcggctta tttttcgaag aatctactcc tggaacaccg tgtatcagag
    14041 gttgtgaaag agacgttcag ccttgagcga tgataaaaac tctcacaaac attgcgacga
    14101 gtaacccaca ggagcaataa gaagcgattt tgaaaaatag cttcccatat ttcacaaaat
    14161 tatgccttat tgtcgatagc ctgtctcttg aacgccattt attcgtaaaa aagaactgtg
    14221 acaagacttt tgtcacagtt ctttttgcgt gcttagcctt gaaatttaac tatccataaa
    14281 tacaaatact agggttattc taacttcttt tattgcttga atcagtccac aagaaaaaca
    14341 gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat tccttctttt
    14401 taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca atctctttta
    14461 cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga ttttgaacaa
    14521 aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag ttagggttat
    14581 aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct gtgtagacga
    14641 tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct tgagagcatt
    14701 ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg ggatgataat
    14761 tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa tctactccca
    14821 ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact tcccgactca
    14881 aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa ttcttcactt
    14941 cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc ggatatctaa
    15001 taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt gaacttggac
    15061 cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc ttgctttcat
    15121 tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag tattctttta
    15181 cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct gcatcatctt
    15241 tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc agcattcgat
    15301 tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct
    15361 ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa tagcagtcat
    15421 tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt tctactcgat
    15481 ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta actaagtgtg
    15541 cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc aaatctagtt
    15601 ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga gaaatcggac
    15661 gggcaaatct gtccgaaaat aagtaggaaa gaagaccaat gagtcctacc agtgttaaag
    15721 acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca ctagagtcaa
    15781 cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac gttttatctt
    15841 tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat acagttggtt
    15901 gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa
    15961 gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct atcgtagcaa
    16021 ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt
    16081 tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt tgatactttt
    16141 caatttcctc ttcggtcagt tgactcaacg atccattgat tactccccat ttaccagcca
    16201 aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa tttagttctt
    16261 tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg aatatgtccg
    16321 agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta attttccatt
    16381 caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa ctcgattcga
    16441 tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt acggctccta
    16501 ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt ttcttcctct
    16561 cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat ctaattaaat
    16621 gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt aatttttaac
    16681 gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt aatatgatga
    16741 atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga tcaaagatgt
    16801 cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata acagcagttt
    16861 aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga attattcgcc
    16921 aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac tatttttctc
    16981 aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag gcatccattc
    17041 agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg atttaacaga
    17101 aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg atgatcagcc
    17161 tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa
    17221 tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc aggcagtaga
    17281 ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga aaaattttcg
    17341 ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt
    17401 caatccagcc tattttttaa caggagatta cagcttagaa agcggcctcc aaaacatgga
    17461 atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg acgacatagc
    17521 gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga acatttctct
    17581 gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg
    17641 taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt tgatcgaagg
    17701 caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc gtgagtcgat
    17761 caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc ataacacaga
    17821 aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga agcacctgta
    17881 aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg gcagattcag
    17941 cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa
    18001 ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt acaaggactc
    18061 agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca aatgatcaat
    18121 ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt ggcctatatc
    18181 gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt aacagatact
    18241 cccccacaga gcctcccggg caaactgaca gcctttttat accgacgttt caaattaaac
    18301 aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa
    18361 gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt tgagtggtta
    18421 gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc
    18481 gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat ggtcaaagca
    18541 gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca actaccattc
    18601 aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg tgaacgtaag
    18661 gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt ggcggaattg
    18721 gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga tcagttattc
    18781 aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat ttatgctgac
    18841 caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc gatttcatta
    18901 aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta tatcgaagaa
    18961 caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct tatgtatcga
    19021 ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac tgatgcttgg
    19081 tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat
    19141 ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa gatcgatcaa
    19201 aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc ttgaaaccct
    19261 gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa cccctattcc
    19321 aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag atatcccgca
    19381 aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca aatatggcta
    19441 tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca ctcataatgt
    19501 aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc atccaagtca
    19561 ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa aagtagggat
    19621 tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg ctgaactgat
    19681 cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata ttacgatttt
    19741 aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc gagaaatttt
    19801 agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat taggttgtga
    19861 gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa
    19921 atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc tattagaaga
    19981 gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta agttgaatgt
    20041 cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc ctttacttgg
    20101 tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag aagtgccaga
    20161 aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag tttttgactc
    20221 aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg aaccagttta
    20281 cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag ataagtcttt
    20341 aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa
    20401 aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg tagctgcctc
    20461 tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag ggactccgtt
    20521 tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg aacgcaaagg
    20581 tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag taatcttaga
    20641 atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa
    20701 tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt cataaaacag
    20761 tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt aaaagacgat
    20821 tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga ttatcactgt
    20881 cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac agaagcttgg
    20941 ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa
    21001 atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa
    21061 attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt cttcggtatt
    21121 gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa tgaaattttg
    21181 gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt
    21241 acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca tgaagaacca
    21301 gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga aaaagaagga
    21361 tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac cacctatcaa
    21421 gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg atgtcgtgtt
    21481 tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt attagaagag
    21541 atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga tgcttatcgt
    21601 acagaaacac tgatacattt gacccacttc tatcattctc ataattggac gatgcagctg
    21661 cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg gcccgatacc
    21721 ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt attgaaccgt
    21781 gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc gaatgactat
    21841 cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa actgcagtta
    21901 ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt gacacaattt
    21961 gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag aagtttcctg
    22021 tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg cgaaatggtc
    22081 atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca agcgattagt
    22141 tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag taacagtaac
    22201 gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta gagaagaaga
    22261 cggaccaaat ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt
    22321 atgaaagaag agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa
    22381 aagatgaaaa caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac
    22441 ccgagatcca gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg
    22501 aaggaaggtt agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat
    22561 ttggacataa cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt
    22621 taaaaaatct gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac
    22681 tgacatctgt gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg
    22741 gagactatcc aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg
    22801 tgttctcaag atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct
    22861 acctgcatct tatacctgat acttgtaaaa attacccaga aggactaaaa gacaataccc
    22921 actttagtcc agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat
    22981 cccgtctgcc tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta
    23041 ggcatccgtg cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca
    23101 cagcttcact tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa
    23161 ttaactgaca tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat
    23221 atcgaacttt ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt
    23281 gcacaagcat tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag
    23341 atgattgcta cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa
    23401 gaagcttatc agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt
    23461 ggcgtaactg ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct
    23521 aaacgtttaa tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat
    23581 ttgatgcata aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat
    23641 ttacatggtt atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc
    23701 cgcatggtac ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa
    23761 tatgagcgtc ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa
    23821 gcaatagaac acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg
    23881 aatacagaaa agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta
    23941 tcagggaaat tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc
    24001 atcttggtta atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc
    24061 ttctcagttg caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa
    24121 tatattgacg aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc
    24181 tggactaaga gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga
    24241 attgcaatct ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt
    24301 gtcctctcaa gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg
    24361 tatcacgctt tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc
    24421 cgcattcgtt actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca
    24481 agaaatggaa gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg
    24541 agctcatggg ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta
    24601 tgaaaaagga ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag
    24661 ttttcagata tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg
    24721 catccaaccc ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt
    24781 ttctggaatg atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat
    24841 tggcgaactg ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca
    24901 tttggatgga aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc
    24961 gattatcgat catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc
    25021 atctggctct ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc
    25081 aggaaaaatt tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa
    25141 ttatttagcc gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat
    25201 cacttatggc ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca
    25261 actttcttca ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg
    25321 caacaagctg tctggtggcc aaaagcaacg tatctccatt gcacgagcgt tgatacgtca
    25381 gccaaaaatc attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa
    25441 aatccagcaa gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg
    25501 gctttcaacg atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga
    25561 aataggtact tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga
    25621 atgacaaaaa ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg
    25681 gtgttctcct gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt
    25741 tttttgaaat tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa
    25801 tggtgttcaa gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga
    25861 acgtctcttt cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg
    25921 aactggtgtc atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt
    25981 ttgcatcaag tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt
    26041 cattgcatct tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg
    26101 catcgcttgg aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta
    26161 cgcttctttc aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc
    26221 gctatcgtag atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt
    26281 aacatggggt tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt
    26341 tagccgcttc accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc
    26401 cttcttttgc ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat
    26461 tagaatttcc tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg
    26521 catctgctac actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt
    26581 tgaagacttt cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt
    26641 cttcgtaacc catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt
    26701 catttgcttt tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat
    26761 aacctgcagg attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg
    26821 tcatttcatc ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga
    26881 cgatcaccgc tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac
    26941 catcattggc aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca
    27001 gtccaccaag ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt
    27061 ctgctttatc atatcctttt tgtaaaccga tctcatgcaa aagccataca gtaggcaaat
    27121 tcaaactttg tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat
    27181 tatgcgcatc gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag
    27241 cttccagtgc tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca
    27301 tctgtgtagc aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt
    27361 gtacaccacc tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg
    27421 aagcatttgc tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat
    27481 cctgatctaa agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac
    27541 catactcgtt aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag
    27601 aatctgaatt atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt
    27661 gttcttgttc ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc
    27721 gatcattcgc attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc
    27781 ctgccaaagt agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc
    27841 tggaagcgtc ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg
    27901 tcaagatttc ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg
    27961 cttttcgatc aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga
    28021 tcgtacttcc tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg
    28081 caatcccttt gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta
    28141 ctgcatcttg gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt
    28201 acagtgtgcc agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg
    28261 attttaacgt ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta
    28321 agacacagat caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc
    28381 gctttcgcca tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca
    28441 aagcagcttt tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat
    28501 ttgattcaat cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta
    28561 gttcaatatg tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg
    28621 taagtttttt cttaaatatg ttttaaaaat ggtacaatta gtctgcgatt gcactgaaag
    28681 gagtacactt gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg
    28741 cgacttgctt gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg
    28801 taaaaacgtt tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga
    28861 ccagatcaca ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga
    28921 tgcgaagcac atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc
    28981 aggaatgaag actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc
    29041 tgcttatctc gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac
    29101 aagcggtgcc gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt
    29161 ggaacaaaaa atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga
    29221 tatgaccatc accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga
    29281 tgataaaaat ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca
    29341 aacacaagtc tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc
    29401 tcataaggga catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact
    29461 gatgctccat gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg
    29521 taaagcttta cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat
    29581 aatgataaga ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct
    29641 gctaacagca aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat
    29701 tcacttactt gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt
    29761 ttttgaagga gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct
    29821 aacgtcaacg tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca
    29881 ggatgatctt gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct
    29941 gctgctaatc cttctaaaca atctttatga taaggacaag taccttcata atggtcatct
    30001 ggatggcgtt tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct
    30061 tcattgatca cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt
    30121 tttccttgtg ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc
    30181 caagcagcag gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac
    30241 ccttttttgg gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc
    30301 gggccgaatg agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc
    30361 tgatccaatg tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca
    30421 ccatctgaaa ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt
    30481 tttcctcctc gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt
    30541 gcttcgttgg ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt
    30601 ccaatttttc taaatgacta gacatattga aacctcttac tactacttta tcactaaact
    30661 tgctgcgttt caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt
    30721 ttgaaatcaa tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg
    30781 cgtatgcatg caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat
    30841 tgaaccgatg ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta
    30901 gttctcccac actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt
    30961 cattcaaacc aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg
    31021 cttgctgatg ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct
    31081 tcaccacttc aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca
    31141 agacacgtaa gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt
    31201 tactatcttt tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc
    31261 gttctgcttt tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt
    31321 ctgcagaaac agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat
    31381 ctactaatac ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga
    31441 aaggttgttt aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat
    31501 tattcaactt tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg
    31561 tcaaatgtag agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt
    31621 cttctgcttt cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt
    31681 tttctaatgg taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt
    31741 tcggcaaatc ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc
    31801 cactttttgt ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt
    31861 gttcaggtat tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac
    31921 cagttttatc cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc
    31981 cggtcgtatc gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa
    32041 cttcatctgc tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac
    32101 cgcaaatact gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat
    32161 catggggata gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt
    32221 ctagctgtcg ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat
    32281 tggctaaagt ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta
    32341 agtcttctgg cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa
    32401 actcgtaatc tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta
    32461 ccggttgatg atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg
    32521 catccgctaa tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat
    32581 tcccgttgct ataccaattg gcaaatagca gaccaaagat tgctgtttga tcaggtcctt
    32641 tccaccacat ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa
    32701 agccaattgg ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta
    32761 attgaggcgt ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg
    32821 ttccccattt acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc
    32881 tgataagaaa atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga
    32941 ttgcttgttg gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa
    33001 tcgtttgccc atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta
    33061 gatcgtctat caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac
    33121 gatcccagtg gctatgagaa atgatatata ctttcttttt catttacgat acctcctact
    33181 tttctactct aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag
    33241 aaaaccattc tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt
    33301 gtgttcctgc gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg
    33361 acgttgtcat tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg
    33421 aggatccaat tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca
    33481 atgttcttct tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg
    33541 cggcgatcag attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta
    33601 cttcatatgc ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc
    33661 cctcttggat ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta
    33721 tcgtttgaaa aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta
    33781 ccagatatcc ataacggcaa gtatcatcgc taggacggaa accagaccat gtcattcctg
    33841 taaaagctgt ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt
    33901 ctcgtacaaa tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga
    33961 tttttttgat accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt
    34021 aaagaagata ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc
    34081 agatccaatc gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt
    34141 tgaaggcatt cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc
    34201 tgatcatgtc agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg
    34261 cagtagagtc tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt
    34321 cattttgctt tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg
    34381 cccagtccgc atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg
    34441 attttggtat atctttataa gccactaata aaaccttctt tctatttgat atatctttaa
    34501 agattatact tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact
    34561 ttttcatcca ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgaat
    34621 caaccattgt accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta
    34681 cctgtagaca gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga
    34741 atcacttcca aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc
    34801 gggaaaggca gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa
    34861 acacgacgga ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc
    34921 aagggattga accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac
    34981 tttctacaaa ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat
    35041 gctcttgata cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct
    35101 gtcgtcatat tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat
    35161 ttccaaggcg gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct
    35221 tatttgtttg gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt
    35281 gaagcattgg acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca
    35341 ttagatgaat tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac
    35401 gatgttgtcg ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa
    35461 gatttttcaa ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg
    35521 acaaccatcg cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact
    35581 tggttagaga caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga
    35641 caatcaacaa aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc
    35701 gaatcagcca aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat
    35761 gaattatttt gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga
    35821 ccgcgttttt caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag
    35881 tcatatgctg tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca
    35941 aagttcttac cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt
    36001 acgttatggc atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga
    36061 ttacagcaaa aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt
    36121 tatagatcct tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga
    36181 tttttctttt tattttatat tgagatttga atatccttta acaattgatg agttagctgt
    36241 ttccaacaaa aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat
    36301 ccgatttggg acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca
    36361 aaaccaaagc aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga
    36421 tcgtatacag ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata
    36481 tcgggtgttt ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca
    36541 ctatgatacg actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg
    36601 ggatacgtat aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga
    36661 aatgctggaa ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc
    36721 tcctgatgaa cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc
    36781 tgtaaaagat atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc
    36841 tacggttcaa agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata
    36901 tggctatgtt cctaatcact accacgaatc agtcaatcac acattagact atgcttatag
    36961 cgatttttgt attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata
    37021 tcagcagcaa tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc
    37081 aaaagataaa gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta
    37141 tgcagaagga agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat
    37201 cggaaaatat ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc
    37261 cccaacgttt gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc
    37321 gatagaattt ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt
    37381 cagttatata ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac
    37441 cttcgacgat tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg
    37501 gtatatcttc aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat
    37561 cggtatgcca ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat
    37621 tgaagcaacg cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacaa
    37681 agaatatacg aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta
    37741 ccaattaggg atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt
    37801 atcaaactat aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    37861 gccaagcaaa aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa
    37921 gcttttgaac accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag
    37981 agacaaatgg caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca
    38041 gctgattgaa caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa
    38101 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
    38161 actggctttt gaactccgtt tatcgagagg tcatgaaaaa gctgccttgc ctcaagtaat
    38221 aagaacagcc caaaattaga tttctagaca attttttata agttatttca ttacattggc
    38281 tttttctttt atgattctcc actgttttct cggtactctt taggggatct gccgacgatt
    38341 ttcttgaata atttagagaa atacgtggtg ttgttgtagc caatctcact tgctatctca
    38401 ttgatgtttt gattactgtg caaaagcagt tgtttcgctt tttccatacg taaatggttc
    38461 aaataagctg aaaaactttt tttcgtttct tttttgaaca gctgtcctag atacatgaca
    38521 tttaaatgca ggttttctga cacttctttg agtgtaagtg gttcttgata atgctggtgc
    38581 aggattacga ttactttttc cacattgtca ctgtactttt tctgtctctg atgctttttg
    38641 atgaatgaca gaagtagctg ctgaagttct tgcaccgttt ttgcctgatg gattttttcg
    38701 attccttgta aatattcctc atcttccaga tcaatcaatt cccgttgaat ctccataaac
    38761 agtaaaaaac tggaatatcg tatatcttcc ggcatcatgg ctgctaactg aaactgttca
    38821 aaaaaatcag tgatcatttt ttgagcgact tccaattgtt tattttgcag cacgcgtctg
    38881 aaacttgaaa aatcgatggt ttgatctttt gtttctgctt gtactgcgta aaaaacatgc
    38941 tcgtctttat ctccataaaa ttgatgcaac tgtagattgt ctttcgcttg ttggaatgtt
    39001 tcagggattt tgtccaaaga atctgtctct tccccgatac tgatcagcca ttcctgatca
    39061 ggaaagtttt cttgaacaaa acaacagaag ccatcaactt catctgcctc caacaacaac
    39121 gtgatcaata cacggtctcc gtaatttcgt tgatagtaaa agatttgttg ccgttcttgc
    39181 aaccatctat tgataagagt atcatgagtt cttgccagtt gaatcaatac tatcctatgc
    39241 tgcttctcac ttatttgtcc tattatttct tcttcactta tttcatccaa ttcttcattc
    39301 aaccattgac tgaataacag ttcttgatag atttcttgtt gatttttttg ttcattttgc
    39361 tgatctaacc gtgttttgat tttcttgagg ctttcaacca attcgaactt attcacgggt
    39421 ttcatcaaat aattcacggc gcctaattgc atacctcctt tcaaataatc gaattcttgg
    39481 tatcctgata aaatcataaa ttcaaaatta tgacgttctt tttgagccgc ttcaataaat
    39541 tccagtccat ttatttctgg cattgtcaca tccgttacta ctaaatcaat ctgtcgctgt
    39601 tcaagtacag ctagtccttc tttggcattc tctgctgttg ctactatttg aaagcctaaa
    39661 ctttgccaat caattatctt ttttaaccca ttcaaaatca tgtattcatc atctaccaat
    39721 aacacttgat acacgctcat ccctccttga atgtaataga aacggtcagt ccacctgtct
    39781 cattattctc aagcgacata taataactgc tgccgaagct ggcacgtaaa cgttcattca
    39841 cgttttgcag accgatagaa ccgtgaagtt ccactttagg atgagacagt ttttcttcaa
    39901 tctgttttag ccgtttttca gtcatccctt ttccattatc tttgatgatg atccttaccc
    39961 gtttgccttc ctgcaagact ttgacactta gcgcattatc gaatcgagta aaatcaatac
    40021 cgtgtttaaa ataattttca actaatggct ggatcacaaa tttaggtact tcgatctttt
    40081 ccaaatcagg atcgatcata aaatgatagg ctactcgatt aggataacgc atttgataca
    40141 aatagacata tttttcgcaa aagtccagtt cttcttttaa agtgatcgtt ttctcttgat
    40201 tcgtattgtt tcgaagcaaa gcggaaaacg catacacaac atctgctaat tcttcacttc
    40261 cttcgctgat tgcatacatt cggatatatt ctaatgtatt atataaaaag tgtggattga
    40321 tctgtgcttg aagcgcccgc atatgtgcgt cttgctgttt gatttctaat ttgtagatgt
    40381 cttctacaaa ctgattgatg ctatccaaca tttcgttgat accgattgaa agttccttca
    40441 gttcatattc cgtgtttgtt atgtcgatcc gcgtctctaa attccctgct gctgtttgat
    40501 ccatggcctg catgacgata gatacttgct gtgaatatcg tttgaatgtt ttataaagaa
    40561 agataagcag tatattgtct aaaataatga taccaatgat cagcggggca agattttttc
    40621 ccgtttcgat ttttacactt tttttatcta gtacagctaa tatacgataa tcatcagaca
    40681 gttcttggtg ttgaacaaaa ttgttttttt ccagatcgtc aatcggcaat tgtgaatttt
    40741 tcttcagact ctctgtgatg atcatttctt gtctttcaaa cgtttcttta gctattttat
    40801 tatcatggaa tgtgtatagt cgatttgtcg ttccagaaat catgtataaa gagagtccat
    40861 tgaatgacgt tagattttct aaaatcatat ctagttcttt cttttgaaaa ccaataaaaa
    40921 tacttcctga ctcagctcct ccttgtgtga taggcaactt gatataaaag gcgtctagta
    40981 attttggtgt cccctttttt atttccccac ctttgttttc tctagaagat tcataatact
    41041 ccggtagttg attcaacacg atatatatcg cacttagttc ttcataattt gcgtagagat
    41101 ctttgatttg atttgggaaa gaaactaatt catcggtact gttttgctgt tcgtatacat
    41161 acatcagata ctgatctatg ggttttgtta agtaggtcgt cacattttcg atcttttcgg
    41221 aattccctac aagctggttt ttgatgatcc tactcaaggt tgttttatca ttcagcattc
    41281 tgcttgtgct ttgaacggca tcacttgtca tgttttctgc ctgtttttga ttttgttccc
    41341 aagtatggat actaaaaata acggtagcag tcatcgttac accgatcatg ataaccgcgt
    41401 atttccgcaa cagacgattt agtaaggttc cttttgaaaa tagaatccga aacttattca
    41461 taactttcca actgtttatc taattcttca cgccaatgtg ttgtcatcgt accattccaa
    41521 accgtcaaaa gaccaatgac accaaacagg atcaatcctt tgaattgcca agtcacgcct
    41581 aaaattatac cgctaccaat aattattttt agaaaagacg aaaagctgga aaaaaccgaa
    41641 ataaaactta gctttagcag attaggaaca gagatcgtat attcactatc taaaatcata
    41701 ctgtattgat aagtagcaaa taggcagagc aaggcaaaaa gtaaaataaa atcaatagct
    41761 aaaaaaatga gccctttgat ttgaacagat aaatataaat tgtaactcaa taaagcagta
    41821 ccagataaaa ataaactaaa tagcagattg cctcttaaaa aatttctttt atatatattc
    41881 cagcctcttt ttattgtgat ttctttatac tcgaatccgt acaaataaaa tagttcattg
    41941 actgttttcc atgccgcacc taatccaaag atgatcccgc cggcaaatgt atatatccag
    42001 aaatacaagg ttagttttat aatgacccag atccggataa ataactcttc caaaaccttc
    42061 cctaacatat cttctcctcc aaacattaag tttatacttt ctcattatac aaaaaaaggt
    42121 tgggcaatca gcaccttgac ctgattatta gagaaacaaa cggaaaaata gttcctcata
    42181 ttttgtcgtt ttgtgagcga ataccgaggg ttagctgatt gaacaccgtt taccaagagt
    42241 ttgtgaaata gccgcttagt ttcgagtagt aagaaaaatt ttgtgaatat ggctttccac
    42301 atttttacat atatttagct taataccgag aaactggctt ttgaacaccg tttattcgga
    42361 acaagaggtt gtgacacgtc ttttgtcaca acccctttgt ctttattcat tattattctt
    42421 gtcggaactc atcatattgt ttttgcattt ctttcaatac cttatcatag ccagccttgt
    42481 ccaaagcgtc tttcaatttc ggtaatgttt catctggatc aacagtccct gtattcaatc
    42541 catctaaata ttgattcatg acattagaaa tattacttaa ttctgttttg acactgtcag
    42601 tgttgaagct gaatcctaaa atcggtgaag tttctgcatc agcaatcgat tgatcacgtt
    42661 tggcaatcat gtcatccgta atagactctt gtgtatatag aatcttgttg tttcctgtat
    42721 tccaagcaga catatgtgta tttggctgat aaccatctag caatttgatt ttatcatctg
    42781 aaccatcgac tttttcccat gcttctccct cgatccccca aacaagccca ttcaataatt
    42841 ctggatcact gttaagcagc cctagaagtt ctactgattt ttctttattt tttgaagtat
    42901 ttgatacgac aaaatttgcc atttgtgctt gagctgtaga ttttagtgga acagtaattg
    42961 cttttgagac aagttcttgt tttgctgcat ttgtcaaaat tgtatcgcca taatcatacg
    43021 gtccttgtgt ttctccacgc attagccaag tatttccttc tagtggataa tccttattgc
    43081 tcgttgcagc gtcacttgga atgagtcctt gtttgtacca gctatgcatt gtacgtaaca
    43141 gtccgatata cgtttcatta tcgtattggt tgatgatttt cgaatcatct ccatccagat
    43201 caatagcaaa tggtaacgta ttccctaaag gataatcaaa gttcccttgg actttgaagc
    43261 cttgtccaat ggcaaaagct gccgtgtttg gttctttttc atggaattcc ttcaagactt
    43321 tttctgcatc agcgtaagat tggattccat caattgaaag atcgtattta tctagtaaag
    43381 ctttgttaaa agtaagtact tgttgagcaa agacatttcc attgactggg aaagcatata
    43441 attttccatt gaccagattt cctttaatat atgcttcatc taaatcatta taagcttttt
    43501 cagcgtactt aggcgctaat tcagtcaaat cagcaaaggc tcctttttgg gcattggaaa
    43561 catagttgtt cgcaaaggcg atatcataat tttccccgga agaaatgatg acgttcattt
    43621 tcttttcgta atcgccccag ccaatgtaat tgatattgac ttttacaccg atctcttttt
    43681 cgattcgttt gtttgctact tccatcaatt gatcgaaatt atctggttta tctccgattt
    43741 gatacatctg caaagtgtcc gaattgctag cttctgcctg gtctttagaa ccagaacctc
    43801 cgcatgctgc taatgttccc aacaacaacg ctgaaacacc tgtaaaggct agtttcttcc
    43861 ataacttcat ttttcttcct cctaatttta tgatttatat ttttgtttct ttattctttt
    43921 actccgccga ttgttagacc tttgacaaaa tgcttctgga aaaatggata gctgatcgca
    43981 ataggcaacg tcgagatgac tacgatcgcc attctggcag attctcctgg aactgaggct
    44041 aatccgcctg tcaattggct tcctgctcct gaattttgtg tcagatattg aatattactt
    44101 tggattttca tcaaaagata ctgtagtgga actagcttat catcttggat gtatagtaaa
    44161 gcattgaacc agtcattcca gtaacccaat gcggcaaata aactgattgt cgcaatacct
    44221 ggaacagcta acggcaaaac gatctgaaca aatattcgca gttcgctagc tccatcgatc
    44281 cgtgccgatt caataatcga atcagagacc gaacgtttaa agaatgtgcg catcacaata
    44341 atattgaagg gactgacagc cattggcaag atcaatgccc aaatcgtatc ttttaactga
    44401 agaagatttg tcataacaag ataattggcc accatccctg gtgagaacaa catcgtaatc
    44461 aaacaaaaaa cggtaaaaaa acgacggaat ggaaagtttg accgtgaaat cgcataagca
    44521 tataaagaag tagccgtgct attgatcagt gtacctaaaa cagtaacaag tacagtgacg
    44581 aaaagcgctt ggaaaatttt atcctgcatc tggtcgaaca agtacgtata accaaatata
    44641 ctgaactttt ctggccaaaa actgtacccg tgagtcgaca atatggactc atcggtaaaa
    44701 gagatggcga taacaaatat aaatggcaaa atacaggaaa tagcaaagag tgcaatcatt
    44761 atattagaga aaaaattagc cgtcttattg aaagagcgga cttggactct ctccactttc
    44821 ttttttttca tttttttcct ccttagaata atgcagaatc gctatcaaaa cgtcgggcga
    44881 tcgtatttgt gatcatcaac aagacaaagc cgacaactga ttggtaaagt cctgcagcag
    44941 ccgtcatacc aaaatctcct gtggatgtca ggccattgta gatatacgta tccaaaactt
    45001 gagtcacatc atataatgca ccggaattcc taggaatgtt ataaaacagg ccaaaatcag
    45061 ctcggaaaat gtttcctacg gctaaaatcg ttaaaatcgt cattagtgga ataagttgtg
    45121 gtatcgtcac atgtttgatc tgctgccatt tgctagctcc atcaaccata gcagcttcat
    45181 aataagtcgg atcgattccc ataacagatg caaagtaaat aatgctgtta taaccaatcc
    45241 ctttccatgt accaatgaac accaagataa acggccaata tttcggttca ttataccaat
    45301 tgacagcttc tcctccatgt ccagtgatcc actgattgaa tattcctttg tcaggactta
    45361 aaaaagcgta aacaaagtaa ctgataatga cccaagatag aaaataaggt agtaaggaca
    45421 tcgtttgata aactttaacg agtcgtttat ttctcaactc gctcataatg attgcaaata
    45481 cgactgagat cagcagattc aaaaggataa atgctacatt atataaaatt gtatttttag
    45541 tgatgagaaa tgcttgatcc gaagaaaata aaaatttgaa attttcccaa cccacccaag
    45601 gactttccct caagctagcc aagaacccat ctgctgaaat atgaaaattt ttgaatgcta
    45661 cgacattcgc cattacaggt atgtaaaaga aaaaaatcat ccaaagcata cccggtacag
    45721 ccatcaggac aagtgcttta taacgccata gattcataaa aaaatgattg aacccctttc
    45781 gttttttcat ctaaaaaccc ctttcgattc attcgcaacg taattatagc gctttcaata
    45841 atgagaataa ataaacaaag tatagggaaa atgaaacaaa gtataggaga ggttgtgaaa
    45901 tcagcgtttt ggcctgagta ttagagaaaa aaacgggaaa atagttcctc atattttgcc
    45961 gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaga ggttgtgaat
    46021 tcagcgtttt ggcctgagta ttagggagat cgtgtcctta ctatctaact ctgagtcaca
    46081 aggaacaatg gggactgtaa caaaagtctt gtcacagtcc cttattccga acaaacggtg
    46141 ttccagaagt agcttctacg gaaataagcc gaaatttcac aaaaaagcca tccgaaagtc
    46201 agacggacaa gtctgctttt cagatggcgc tacaactact ctgcttattc ttttagctat
    46261 ccattcaatt atttttctct cacttgttct agctcttctt tggtcatggg tcgatattgc
    46321 cctttattca aatctttagg cagccaaaat tttcccatac ggattctttt taaatataag
    46381 acttcttttc ctactgcttt gaccattcgc ttaacttggt gaaactttcc ttcatgcaaa
    46441 atcagacgga taattgaagt ttctgtctct tgatctactt gatcaatttg gagttttgcc
    46501 ggcaatgtct gctcatcgcc atcaataatc agtccttctg caaataaacg gatatcttct
    46561 tctgtcatta taccgctaac ctcagctaga tattctttct ccacatgttt tttaggtgat
    46621 agtaactgat gggcaaacac cccgtcattc gttaaaatca agaggccctc tgtgtcttta
    46681 tctaatcggc cgactggaaa aagatcttcc cgataatcct ggtcttccaa taaatcgatt
    46741 acggtttgat catgattatc ctgagtagca gaaatgactc ctgcaggttt atgcaaaatg
    46801 tagtaaaaat ctttttgata cgtaatgatt tctcctaaat aagtaaccgc atcttcgaat
    46861 tcatttactt gcgctttatc gcttttcacc acttgtccat tgacttcgat cgttcctttt
    46921 ttgattaatc ctttgacttc tttgcggctg ccaagtccca tatctgctaa aaacttatct
    46981 aaacgcatgc gtttccccca tttcaaaaga gcatcaatct agctcttctg attcacttat
    47041 tttattttta atttatgtcg taaacgcgct attcctggtc ctagtagtct ttctgccaag
    47101 cggattttca aagctaaata accatacact aaagccccta tacctgctac tacaagaatc
    47161 aacaatagag actggaattt gctatcttcg tttaaaaaag caccaaaaat catctttgtg
    47221 atccacgaag ccaaaagcat aatcaaggta agaataaaga tcagcaatgt cctgcgccag
    47281 acaaaacttg gtttaaagcg ggctacttca tggattctct ttaagatcaa ggtacaagac
    47341 acagcaaatc cgatcattgt cgccaataaa gggccgtata cttcaaataa acgaatggct
    47401 gggtattgaa gaaggagttt cactagaaac ccaataacca aataattaat tgccgccttg
    47461 ttttcaaaca ttccttgcag catgttggaa actagcatat atagtcctag gaagagtcct
    47521 acaaaacttg cttggatcag tacattactt cctagagaat ccggtgtata aaataatgta
    47581 tttagcggat aggcaagtaa cataacacca aatgtcgccg gaaacataaa aaatgaaaac
    47641 aactgtaaat ttccgcttgt caattttgcc agacctactc gatctttcaa cgtaacagct
    47701 tctgtaatca acggtaaccc agtagacgcg atcgatgttg ccaatgcaat aactaccatc
    47761 gtcaacttat ctggattggc actaaaaatc gaaaaaagat ccagtaactg tgcattggaa
    47821 tactcggtgg atgcagacat gatcttcata aaagtgaact gatcgactaa tttgaagatc
    47881 gttacccctg aacccacaat aataaacgga atcgcttctt taacggtatc taacaacaat
    47941 tccttcgttg caatatttat tttgttttcg ctgtagtgaa taaacgccga tgtatacgct
    48001 tgatgtttct tcaaaaaata caaaaggaca agaatacttg ctagcatacc gataaatgcc
    48061 gcgaaagttg attgcgtgac cgccgtcaca tagtcaccat ccatgacttt catgatgatg
    48121 aatgtagcca atagcatata aaaaatacgt gccacctgtt ctacaatctg cgacagcgca
    48181 taaggcatca tttcttgatt gccttgcaaa taccctcgta tcacactcat acaaggaaaa
    48241 accaatacag cgatactcaa agaacgcatc gttggaatca gttcttctcc gccaccagaa
    48301 gcatgagcta gccaaggaga ggcgatgtac atgaacaaag caaacaacag gcctaacccg
    48361 cccatcatct gtaatgcacg gataaataat cgtcgtgaag tgccatattc attcaatgaa
    48421 ttatagcgtg cagtttgttt tgcaatcgca gcaggtattc ctgctgtaga gatcagtaga
    48481 aacaatgcat atatattgta tcccatattg aataatccat tggcagcctt agcattttcg
    48541 cccatccaag cataccaggg aatgatatag acagcgccta gcaatcttga aatgatatta
    48601 ctggcggtca tccaagcaga gcctcgtgcc attttttctt gcggtgttaa ttgatctaat
    48661 gctggttttc tttgattatc catcggtcag attccaactt tctacaaaac attactatca
    48721 ttttaattgg taaaacagaa actggcaatc gattttatat aacttttata agaacaattg
    48781 tttttttgaa ttcggtttcg tttttttggt aaagtaaaga aatatacagc cttttatggc
    48841 taacatttat aggagatatc aatgacacaa gaaataaaaa aactacccgt tatattttta
    48901 ggtcttttct ttatcacagt tggattgaat tggttcttgc tccctcacga tatagcttcc
    48961 gccggagtgg gcgccatcgg acatttagtt gaaacggcct tttctatcca acgctcagtt
    49021 actgtttgga gcatcaatct tatgatgctg cttctagccg ctgtcctatt aggaaaatca
    49081 gtatttttta agactgtagc gggaagtctg ctttttcctg ttgttttaga gattgttcca
    49141 aaagtggaaa tgatctcctc acatttcttt tcactgattt caggaagtct tttgttcagt
    49201 cttggggttt acacattgta cacagtcggg gcctcaaatg gcggtataac gataccgccg
    49261 attattttcc acaaattttt tcggcttcca atgccgcaag gacttttact taccaatagt
    49321 cttatcgtct ttttgaatta ttatgttttt ggattgttgg agacactatt cgttctccta
    49381 tctattacag tcagttcatt gtttatgaaa atactcatcc gcttgtcccc tgtgtcaaga
    49441 aaatttgaat aaagctggat tttgtcatag cggaaacaaa cgatatatta aaaagagtgt
    49501 gctataataa accaagaaat tgaataaagc gagggatcaa tcaatgagct tctctttaga
    49561 agacatccgt catttactat taaaagaaaa tctattaaaa gaatttgttt caacaagagg
    49621 ctggcatctt gatgtaccag aaaaagctga atttcttcag ttaagttatg attcaagaaa
    49681 agctgatgct tctactttgt ttttctgtaa aggaatgaat ttcaaagaag aatatttgga
    49741 ttcagcaatc gaacagggta ttcaatatta tgtatcagaa caaccatacg aaaattcagc
    49801 agtaggaatc atcgtaacgg atatccgtaa agcaatggcg cttcttgcta tggcttttta
    49861 cgactatccg caaaataaat tgaaagtgat cggattcact ggaacaaaag gtaaaacaac
    49921 tgctgcgtac tttacaaaag caatcttgga tcacacaaca aataaaaaaa cagcattgct
    49981 ttctactatg aatacaacgt tagatggcaa aacatatttc aaatcccatt tgacaacgcc
    50041 tgagtcatta gatttatatc gtatgatgtc agaagcagtt gaaaatggta tgactcatct
    50101 agtgatggaa gtttcttcac aagcttacaa aacacaacgc gtatacggct tgactttaga
    50161 tgtcggaatc ttcttgaata tctcgcctga ccatatcagt ccgatcgaac atccgacatt
    50221 tgatgactat ttctattgca agcgacaatt gatcttgaat tcaaaaacag ttgttttaaa
    50281 tcatgaaagt gactattttg atttacttag ggaaacagcc gatctattcc agattccaac
    50341 gatcacttat ggtcgttcgg aagaaagcaa ttatcaagtg atccgttcag ataaaggtac
    50401 tcacggtttt actttatctt ctcatgaaga tcgcttagct atctgcgata ctgcttatga
    50461 tattttgctg gctggaggat tcaaccaaga aaatgcagcc agtgcgatca ttgccgcagc
    50521 actaacaggt gcctccagtc aagatgcaca agacggatta aaagaagctc gtgtaccagg
    50581 ccgaatggat caattgatcc aatccaatgg agcgcatgtc tatgtggact atgcacataa
    50641 ttatctaagc ttgaaaacat tgctgaaatt tgccaaaaat gaacatccag atggccgtgt
    50701 aattgttgta ttaggcagcc caggaaacaa agcaatctcc cgtcgacatg attttggaaa
    50761 agtgctatca gaaacagctg acatcgcctt tttgacggca gatgacccag catttgaaga
    50821 tccgaaaaaa atcgcggaag aaatcaatga agcaattact aatccagatt tgatcgttca
    50881 ttatgaaatg gatcgtcctg aagcgattcg acgcgcttta gcagaaagcg ctccgcaaga
    50941 ttcagtcgtt attgcgggaa aaggcgtgga tccctatcaa aaaatcaatg gtgtagatga
    51001 accttatgaa ggggattatg caatcgtgaa gcggttgatt gaagaataat ggtatttata
    51061 tactagtggt ttcccatttc taagaaaaaa agggactgtg attttttgtc acagttcctt
    51121 ttttcttaga aatcatttta acttttcact agctaattct gctgcctttt ctctttcttc
    51181 atttaataat ttattatcat agaatttgat aaatgggaac cagataatga atgcgatgat
    51241 tgcacaaatg actgctaaca cagcagcttt ccaatcacca ccactaccaa caaatgctcc
    51301 gatcccgact ggtgaaggcc acggcatctg tgcaagcatc ggtttgacga tttccagccg
    51361 aatggcaaaa taagcgagtg tagcagaagc catcggagct aagaaaaacg gaaccgctaa
    51421 atagggattg tagacgattg gcatcccgaa aatgattggt tcattgatat tgaataatcc
    51481 tggtacaagt gaggcttttc ctaacacttt cagctgatca gatttcgcga ggaatgcaat
    51541 aaagatgatc aatcctaaag tcgcacctga tccaccaacg gttacataag aattgttgaa
    51601 ttctcctgcc aacggataat ttgcgccttg tgcgttagct gccatattag ctaatacgat
    51661 cggggtcaag aaagaagtga tgatattcgc tccatggatc cctacgatcc acaatgcatg
    51721 gatcaagaaa taaatcacca tgacaccaag ccatgtattc gtcaactgcg taacaaaccc
    51781 aaatgggata gcaatcattt tgaagatatc cgttcctagt gcaactaata atccattgat
    51841 gatcatcacc acaaacgcaa tgacaaatgt tgggatcaga gcagtgaaag aacgagaaac
    51901 tcctggcgga acaacatctg gcatttttat gacccaattt ctcttgacac acatacaata
    51961 caattcgacc gcaataatcg acataataat agctgtaaag attcctgaag ttcctaaacg
    52021 ttcgacaaaa gagcccatcc taataccatt tactacagtt tctgtctcat tgatagaagc
    52081 aatcaagctg attttaccat cagtcatgat caattcaggg atacacataa agaatgcaaa
    52141 aacagatagt aatgcccccg tgagcggatt aacatttaat ttttgttcat ctcgttcaat
    52201 tgacgttaat tcataagcaa acacaatggc aaagtaaata gataaaatcc ccatcgtcat
    52261 tgtattgacg atcatgtaca agtcactaac tttgaaaaaa gttgcattaa aaaatccttc
    52321 taaaaatggg aacggtaatg gcaaaatatt taaaaccaaa aacatcgagc ctacaatcgt
    52381 gaaaggaata gaagccatcc ccgctgccat gacagcccga acgattttaa gttgcgcaat
    52441 tttccccata ggtcccatca ggtatttctg caaaaaatca aacattagtt attctccttt
    52501 cctaaatata acgctttttc atattgttta atacggatat actgttggtc ttggttgagt
    52561 gaaattttct ttaaacgata ctgtataaat gcactggcaa aaataccgct tacaacaaga
    52621 atgaaaacaa ataactgtcc tccattgtta tttgccataa aaggatacaa gaatgtaaat
    52681 cctgaggtaa acaacactgg aatcactaat aaattaaaga cactctggaa caaaaaatac
    52741 cactttgtcc atggaacgtt cgtttggtgt tttcgataaa gctttacttg ttctcctact
    52801 gcaggcaaaa tagctaaaat cagaatcatc ggtaaactac ttcctaaact tttagctgat
    52861 agaagcagaa tcatccattg catgttcatg aaaaagaaaa aggcagtcag gtaacgtacg
    52921 attagataac gattgaaata catgtttttc aaactaagtt gctttttttc taattcgatt
    52981 ttccttttta ctgtatcctg cacaatgatc cttcctttct atttattttt ttgatacaaa
    53041 taattcattt cctgtgcaac ttccaataat gtcatcgtcg tcatcagatg atcttgtgca
    53101 tggaccatga taatttccat ttctattttt gtcccgctgg catattcttt cagaagttga
    53161 gtttgtgact gatgcgcatg aagaatctct tcatttgctt gtcgcaactt ctcttctact
    53221 tcttcaaaca tctctttacg catcgagcga aatgcttcat gtatcagtgt ccgtgcattt
    53281 ccgctatgga aaatgatatc aaaagaaggc acttgtagtg tttcactgtt tattttttca
    53341 gccatctgtt ttcctcctta ttcgatcagt ttgataaatt tactgctgaa attctcaaaa
    53401 ataggttcag ctaagatttc ctgctgtact tttagctggt caactaacct aacgattgct
    53461 ttagtcacga ccgtaattcc ttcattttcg acataagaag gcgaaactaa aaaaacaaaa
    53521 tgaatttctt gatattgatc ccactgcatc ccagaaggaa tcaatgcaac ggctaccttt
    53581 gtttcgattc caactggaac agcgggatgt gggactgcca ctgtatcact gaatatgatt
    53641 tgtcccatat tttcacgttg ctggatctgc tttcgcattt ctcttatata tccttcttct
    53701 tcatttatgc tcagcttttg cagcaaatca tgaagaattt cttccttttt cggttcgtac
    53761 gtataagtct tgaaaaattt ttcagacaac tgttcggtgt aaatctgctt tttttgctgt
    53821 agagaaaatg ctggtatcgg tctatctgag gaatgatagg gtatagattc tccaactacc
    53881 tttcgtattt tacgaacatc atcttcattc aaaaaaatac tgacatgtaa aactggaatt
    53941 ttgaaaaaca ttgtcgaaag atcaatagaa gaaataatca ggtcgattcc ttttaaagtt
    54001 gtttcattga tctcataata gccttttaca cttaccactg taatattttt accgaattca
    54061 ctgactacgc gattctttaa tagttgggca ctgccgtaac ccgttgcaca gataatcaat
    54121 gcatgtagtt tatgcgcagc tctttctttt tccaatgccg ccatgagatg caaagccaaa
    54181 taggcccatt catcttcatt gatggtatag tttttcaaag ccggcatgtt gctgaaatgc
    54241 ctttttactg cgtcaaagac tttgggattt tctttcttta tctcctcagt caatgggttc
    54301 tcaatgcgaa tacctcggct caatcgaatc atcatcggtg acaaatgatc gagtagtcca
    54361 ttgaaaagct ggagatcctc tactaatgaa aaatgaaatt cctgagacaa tcttttcaga
    54421 actttcgcta attcctttga tagttcctga tcattttgat tttctttgtg attcgactta
    54481 gccatgagat gtaatgtcaa ataagcaatt tcttcttcgg gaaacgatac acctgcgatt
    54541 acctcaatac gttgaacgat ccgctgcgcc acttgatact ctactcgctt agaaacttct
    54601 tgttcgatgc ctaattcctt gatttcaaac ccttctcgga gccgtttgat ccccaaagcc
    54661 aagtgtaaag tcagattctg tatgataaaa tccgaaactt ttaacttagc ttctcttgtt
    54721 tcatcgagaa tgataatgac tagctcttca aaactgatgt ctgggaaaaa acggctttgc
    54781 cctaaatatt ctttcaatga atttccataa tggtttccaa aaaaagtatc catgataaaa
    54841 tgccgtcgat ttctttcttc cccttcgatc catgtccctt gaccatgctt agaaacgatt
    54901 tttagtgaat atggaatcaa tcgctccttg atttcttgca tgtcctttgt caaactggaa
    54961 cgactgatat aaagctcttc tgccaaatca tccataaaaa gaatggcatc ttccaaaagc
    55021 aattggttca atatatactt ttgtcgatct tcggactcat aaaatccttg cggttctcta
    55081 gcatatccag gtactatttc ttgccgactc aaaaacaagt caaatgtcag tctttgtaaa
    55141 atatgcagtt ggtagccata acctggttta gcaatgatct tacctccgtt tttttctact
    55201 aactctttga tccgctgcaa gtaattacgg attgtccgat cagataataa caattcagaa
    55261 gctaaacgtt gactggtgac atactccttc ttgtgatcaa tcaaatatct caacagcaat
    55321 ttttccttct gtttcattat catccactcc tattccatca tatgttctct tcgatcacct
    55381 ctgctaactt ctggatgcct gtagggattg ggacatacgc ttggaaaggg atactaacaa
    55441 caggctttcc aacagattct cctaattttt tgaatttatc caaaaacatc gtcgtttgcg
    55501 ggctgatcag aaataaatca aaatcactat tcttaatcat attatcgcct tctgtagctg
    55561 aaactgcatc tacgagtacc tcttctcttt tttgcttaaa gaaatccgtt gtctttgagg
    55621 ccatcatcga ggatgacatt cctgccgcac aaataatcaa tgcttttttc ataaatcaat
    55681 cactcctttt atttttataa accaagcata tcttgtaagc gttttctatt caaaatatat
    55741 atttccgtag cattgcggaa aaaatgtgta tcatatcttg aataataatt tctggcaccg
    55801 tgatatacta gtgatagaat tttttcagga ggggatttag tgagtatcac agtgaatatt
    55861 ttgtatacaa gtacaaatgg acaagccaga aaatttgcag aagaaatggt cgaaagaaac
    55921 attgtcaaag cgattcgtgc agaagaagga aacgagaaat atgactatta ctttccacta
    55981 gaagatccag aatctctttt actgatcgac cgttggaaag atgaagaagc aatcgaaaag
    56041 catcacaaat ccgaaatgat ggctcaaata aacgaactcc gtaaaaagta taaattaaaa
    56101 atgaaagtcg aacgtttcac tgatttaccg taaaaagata cacatggaat gatagcaatt
    56161 tatcatccat gtgtatcttc actttttcct tattttttat tcgatctcat ctaatgattc
    56221 aaaccgtgtt tttctcttat ttttcggata agtatttcag tcaccccaat atacatgatt
    56281 cctaaacata ccccaaggat attctcaaaa atgcggatcg ttactgctcc tggaaccccg
    56341 aataaactgg cagtgattgc caaagcacca aaagaattaa aaactgtttt atatctatag
    56401 gtagaacaaa cacctaaagc tagaccacct aatataccga cccagttaaa aggaataaat
    56461 tggctgatac caataaataa aacacatcca atcaatgagc caatgattcg gtctactgct
    56521 cgttctttga aaccaatcga catcaatcca tagctagata caatagaaga aaatgcaaac
    56581 cctgcccaca tcaatcgctg aaaagggaca tactctcctg caacgatcaa aagactaatg
    56641 ccaaatgcat agatgagcat ccaaattttt tcctgcgaat acatgccttt caaaaaaatc
    56701 tctttaaaca agcttttgtc tttgttcttt tctcgatgtt ttctgtaaag gagaactgaa
    56761 aaccaacaaa aaaacaaaaa taataagcta cttgtttgtg taaagaaatt cttgttcaac
    56821 gaatctttag gtaacgaata aacaataaag agatatgaaa agccgtacaa agaagcattc
    56881 cccattttgg gatcactagc ggttgccatt aatatgctgc ttagtaaaat aaaatgaatc
    56941 aacatgccca taactgacca tttgatcagc tggacatacg gtgctactaa tagtgagaac
    57001 atgacgattg ctaaacctat gatcgaatgg gaaatcttat aaccaaagtc aacaaaacga
    57061 atactcatca acatacagaa taaaaccaca gcgtatggtt tatgcttcga ttcaaataat
    57121 gttgtaatta taatgataaa acaaatggca aacaatacga tgaataatga tcgtaaaaac
    57181 aatgaacaaa tatacatgca cttttctttt ttagaagctg cttctcgtat tttttgtttt
    57241 aaaataaacg gatctagttg tagagactga taaaaatcca cctgttgtcg tcctttcttt
    57301 ttacttttat tttttacagt catctatgac aggttgtaaa tactatggaa catcatatgg
    57361 cgttgtcatt ttcttgtcaa ctatttttcc tattcatgtt tttataaaaa aacatatcgt
    57421 tttcttgtat gattaaatca ccacaaaata aacaactgag agaacgatat gctttatgaa
    57481 tggttctatc aaaaaaattc tgagatcaat agacaaaaat ttgatgatta cagaggtctc
    57541 ttacgaaacc ttacagaaga aaaaaacatt gatcgttgct gcagttctct tccctgctcc
    57601 atgtgtctgt agaaattgtg gttctgctgt ggtagatgga aataggaaat taatggtggt
    57661 gaaaaacaag aagaaggaaa ccattgtcca ctttgaataa tataatcata tgccaatggt
    57721 tatacgccta aaaaagcagc gctatacctg taaaaactgt cgaagccatt ggaacgctca
    57781 aagttatttt atccgtccaa ggcactcgat ttcaaatcat gtcagacata aaatcacttc
    57841 tttactgact gaaaaagtgt cgttattttt tatttcgaaa agttgttagg tatctttgac
    57901 aaccgttatt cgcacattga aagagttgag aagctattta ccaaagtctt ccaaaagaat
    57961 tctgtcttga gtgttgaggg ttgtgaattt cgttctcacg tttccgttga ggataaactg
    58021 agttttattt ccgcggatgg cgagacagaa aaattaatcg agaaaattta cctacacaaa
    58081 aaattcctcg attaacaaac tatttcctta gctgcactaa tcctaaagaa atagaattct
    58141 tagttacaga catgaacgcc acctactttc agctcaccaa acatgttcta ccaaatgcga
    58201 aattggtgat tgatcgatta cttggttttt ccacgccttt caaggaggcg taacctttat
    58261 tcacgagttg gttgaagctt tccgagacaa aagacccgac ttgtttttct ccttattgac
    58321 aaagagccat taaaactaga gaaaaaatta caactgatcg ctgattattt gtagaaagaa
    58381 aatcaaagta ttacccaata aacttgagtc attaggcaaa aaacactgcg aaactcaaga
    58441 atgattttcg cagtgttttt gtatattttt tattaactag tattactaca aaggtttatt
    58501 tttcacttct caacgcagca caaaattgtc tttacttatt tactggttgg gttcaacctt
    58561 ctctactccg atactaatat ttgtttgtat accccttcta aaatcggtag tttccattca
    58621 attgatgggt actagttgat tttaaaaact atgcttattc aattttttat tttagctcat
    58681 atatttcgtt tctttctaaa ttttatactg atgaaaacaa gtagcacacc caatattgac
    58741 aatataaact ggctaatgat ggtttctcca gtttttggaa gatgcttatt tccactgctt
    58801 gttggattgt ttcctcttat atctgagcta tcagcaactt ttctattctc ttttgttaaa
    58861 aagatcgcca cttcttgttc tttttcgatc ttgaaagtta ctggtgtcgc atccaattca
    58921 taccccgttg gcgcttgagt ttctaccagc tgatatgctc ctggtgctaa gccatctatc
    58981 gccagttttc catctgctcc tgtggttaga cctgactgca gaacggttcc attggcatct
    59041 tgtaactcaa aaacagctcc ttgcaggact tctccgcttt ggtcatcgat ctttgtcaac
    59101 acgacaccgc ctggtgtaag tcgattttct ttcgtcaatt ctaccaccgc tgtctgactt
    59161 ctttcgatct caaactcgat tggtgtcgca tccaattcat accccgttgg cgcttgggtt
    59221 tctaccagct gatacactcc tggtgctaag ccgtcaatcg ccagttttcc atctgctcct
    59281 gtggttagac ctgactgcag aacggttcca ttggcatctt gtaactcaaa aacagctcct
    59341 tgcaggactt ccccgctttg gtcatcgatc tttgtcaaca cgacaccgcc tggtgtaagt
    59401 cggtttgctt tcgtcaattc taccaccgct gtctgacttc tttcgatctc aaactcgatc
    59461 ggtgtcgcat ctaattcata ccccgttggc gcttgagttt ctaccagctg atatgctcct
    59521 ggtgctaaac cgtcaattgc ca
//
LOCUS       DAFEHS010000009        53224 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFEHS010000009
VERSION     DAFEHS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.009"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            380..3187
                     /locus_tag="LP327_000593"
     CDS             380..3187
                     /locus_tag="LP327_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL1724738.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3378..3869
                     /locus_tag="LP327_000594"
     CDS             3378..3869
                     /locus_tag="LP327_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1724739.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3972..4961
                     /gene="manX"
                     /locus_tag="LP327_000595"
     CDS             3972..4961
                     /gene="manX"
                     /locus_tag="LP327_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBL1724740.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4994..5797
                     /locus_tag="LP327_000596"
     CDS             4994..5797
                     /locus_tag="LP327_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBL1724741.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5818..6732
                     /locus_tag="LP327_000597"
     CDS             5818..6732
                     /locus_tag="LP327_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBL1724742.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6815..7189
                     /locus_tag="LP327_000598"
     CDS             6815..7189
                     /locus_tag="LP327_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBL1724743.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7328..7573
                     /locus_tag="LP327_000599"
     CDS             7328..7573
                     /locus_tag="LP327_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL1724744.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7745..8809
                     /locus_tag="LP327_000600"
     CDS             7745..8809
                     /locus_tag="LP327_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL1724745.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9055..9480
                     /locus_tag="LP327_000601"
     CDS             9055..9480
                     /locus_tag="LP327_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL1724746.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9473..9922
                     /gene="nusB"
                     /locus_tag="LP327_000602"
     CDS             9473..9922
                     /gene="nusB"
                     /locus_tag="LP327_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBL1724747.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10019..10873
                     /locus_tag="LP327_000603"
     CDS             10019..10873
                     /locus_tag="LP327_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBL1724748.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10874..12211
                     /locus_tag="LP327_000604"
     CDS             10874..12211
                     /locus_tag="LP327_000604"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBL1724749.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12216..12440
                     /locus_tag="LP327_000605"
     CDS             12216..12440
                     /locus_tag="LP327_000605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBL1724750.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12442..13332
                     /locus_tag="LP327_000606"
     CDS             12442..13332
                     /locus_tag="LP327_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL1724751.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13341..14159
                     /locus_tag="LP327_000607"
     CDS             13341..14159
                     /locus_tag="LP327_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBL1724752.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14266..14715
                     /locus_tag="LP327_000608"
     CDS             14266..14715
                     /locus_tag="LP327_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL1724753.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14733..16415
                     /gene="recN"
                     /locus_tag="LP327_000609"
     CDS             14733..16415
                     /gene="recN"
                     /locus_tag="LP327_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBL1724754.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16470..16586)
                     /locus_tag="LP327_000610"
     CDS             complement(16470..16586)
                     /locus_tag="LP327_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBL1724755.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16659..17615)
                     /locus_tag="LP327_000611"
     CDS             complement(16659..17615)
                     /locus_tag="LP327_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL1724756.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17786..18154
                     /locus_tag="LP327_000612"
     CDS             17786..18154
                     /locus_tag="LP327_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBL1724757.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18324..18755
                     /gene="mraZ"
                     /locus_tag="LP327_000613"
     CDS             18324..18755
                     /gene="mraZ"
                     /locus_tag="LP327_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBL1724758.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18776..19735
                     /gene="rsmH"
                     /locus_tag="LP327_000614"
     CDS             18776..19735
                     /gene="rsmH"
                     /locus_tag="LP327_000614"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBL1724759.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19740..20141
                     /gene="ftsL"
                     /locus_tag="LP327_000615"
     CDS             19740..20141
                     /gene="ftsL"
                     /locus_tag="LP327_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBL1724760.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20142..22334
                     /locus_tag="LP327_000616"
     CDS             20142..22334
                     /locus_tag="LP327_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL1724761.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSVYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22360..23322
                     /locus_tag="LP327_000617"
     CDS             22360..23322
                     /locus_tag="LP327_000617"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBL1724762.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23336..24697
                     /locus_tag="LP327_000618"
     CDS             23336..24697
                     /locus_tag="LP327_000618"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBL1724763.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24723..25811
                     /gene="murG"
                     /locus_tag="LP327_000619"
     CDS             24723..25811
                     /gene="murG"
                     /locus_tag="LP327_000619"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBL1724764.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25826..27046
                     /locus_tag="LP327_000620"
     CDS             25826..27046
                     /locus_tag="LP327_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBL1724765.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27202..28527
                     /gene="ftsA"
                     /locus_tag="LP327_000621"
     CDS             27202..28527
                     /gene="ftsA"
                     /locus_tag="LP327_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBL1724766.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNIKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28550..29791
                     /gene="ftsZ"
                     /locus_tag="LP327_000622"
     CDS             28550..29791
                     /gene="ftsZ"
                     /locus_tag="LP327_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBL1724767.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29808..30485
                     /locus_tag="LP327_000623"
     CDS             29808..30485
                     /locus_tag="LP327_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBL1724768.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30499..31098
                     /locus_tag="LP327_000624"
     CDS             30499..31098
                     /locus_tag="LP327_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBL1724769.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31113..31400
                     /locus_tag="LP327_000625"
     CDS             31113..31400
                     /locus_tag="LP327_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBL1724770.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31449..32231
                     /locus_tag="LP327_000626"
     CDS             31449..32231
                     /locus_tag="LP327_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBL1724771.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32252..32959
                     /locus_tag="LP327_000627"
     CDS             32252..32959
                     /locus_tag="LP327_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBL1724772.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33221..36019
                     /gene="ileS"
                     /locus_tag="LP327_000628"
     CDS             33221..36019
                     /gene="ileS"
                     /locus_tag="LP327_000628"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBL1724773.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36068..37594)
                     /locus_tag="LP327_000629"
     CDS             complement(36068..37594)
                     /locus_tag="LP327_000629"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBL1724774.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37609..38256)
                     /locus_tag="LP327_000630"
     CDS             complement(37609..38256)
                     /locus_tag="LP327_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBL1724775.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38440..38769)
                     /locus_tag="LP327_000631"
     CDS             complement(38440..38769)
                     /locus_tag="LP327_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBL1724776.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38946..39674
                     /gene="sfsA"
                     /locus_tag="LP327_000632"
     CDS             38946..39674
                     /gene="sfsA"
                     /locus_tag="LP327_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBL1724777.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39690..40703
                     /locus_tag="LP327_000633"
     CDS             39690..40703
                     /locus_tag="LP327_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL1724778.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40703..41980
                     /locus_tag="LP327_000634"
     CDS             40703..41980
                     /locus_tag="LP327_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL1724779.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42043..44745
                     /locus_tag="LP327_000635"
     CDS             42043..44745
                     /locus_tag="LP327_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL1724780.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44897..45214
                     /locus_tag="LP327_000636"
     CDS             44897..45214
                     /locus_tag="LP327_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1724781.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45244..45564
                     /locus_tag="LP327_000637"
     CDS             45244..45564
                     /locus_tag="LP327_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL1724782.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45672..47132
                     /locus_tag="LP327_000638"
     CDS             45672..47132
                     /locus_tag="LP327_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL1724783.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47200..47421
                     /locus_tag="LP327_000639"
     CDS             47200..47421
                     /locus_tag="LP327_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724784.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47452..47634
                     /locus_tag="LP327_000640"
     CDS             47452..47634
                     /locus_tag="LP327_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBL1724785.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47634..48047
                     /locus_tag="LP327_000641"
     CDS             47634..48047
                     /locus_tag="LP327_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBL1724786.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48170..49351
                     /locus_tag="LP327_000642"
     CDS             48170..49351
                     /locus_tag="LP327_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1724787.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49422..49586)
                     /locus_tag="LP327_000643"
     CDS             complement(49422..49586)
                     /locus_tag="LP327_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724788.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49740..49813
                     /locus_tag="LP327_000644"
     tRNA            49740..49813
                     /locus_tag="LP327_000644"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49774..49776,aa:Arg,seq:cct)
     gene            complement(49882..51021)
                     /locus_tag="LP327_000645"
     CDS             complement(49882..51021)
                     /locus_tag="LP327_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL1724789.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51320..51955)
                     /locus_tag="LP327_000646"
     CDS             complement(51320..51955)
                     /locus_tag="LP327_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724790.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52068..52703)
                     /locus_tag="LP327_000647"
     CDS             complement(52068..52703)
                     /locus_tag="LP327_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBL1724791.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(52737..53198)
                     /locus_tag="LP327_000648"
     CDS             complement(52737..53198)
                     /locus_tag="LP327_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBL1724792.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        17558 a         8993 c        11251 g        15422 t
ORIGIN      
        1 tttataaaaa taaaagttgt agtggaggct acgaagttta ctttgtcttt ttacacttaa
       61 tatttttttg ccaatttgga atcaggatgt tttgataact atcgttttca attgatggaa
      121 gctatattgt ttgctatcag tgaaataatt atatggtaaa cagtgttctt tttcttgttt
      181 ccattataaa aatcgtatcg ggatttttcg ttcttttaaa ttgaacattt ctatctatcc
      241 gcgtgttatc aacaaaacaa aatcaggtaa atagttgacg aaacagaaat aaatacatag
      301 tattatgttt tgaagcctaa gtacaatagt ctatagaaaa ggagcgatac cattggcaaa
      361 acgcattgac cgaatttata tgtatgtcaa agaaaaaacc gctcatttaa caaaggcaga
      421 atatgatcaa ggagtaacaa cacaggaaat tgctgaagtg ttagggattc agcgaacaaa
      481 tagcagtaaa gatctgaatc agctagtgcg cgaaggaaaa ctgataaaga cagacggacg
      541 accagtacgt tacatatatc aaacactcgt tacgcacgta aagccagtta caaaacatgt
      601 agtcagttac aaagaaacgc cggtctctga agaaaaaaat acactccaaa tagatactaa
      661 ggatatcttt gcaaaaatca ttggagctaa cggcagcatg aaaaattcag tggaacaggc
      721 aaaagctgcg attttgtatc cgccaagagg attaaattgt ttgattacag gacctaccgg
      781 atcaggaaag acttattttg cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat
      841 agatgaggaa acggaactga ttgttttcaa ttgtgctgat tatgccaata accctgagct
      901 tttgatgagt catttatttg ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa
      961 aatggggatt atcgatcaag cggatggcgg tatgctgttt ttggatgaga ttcaccggtt
     1021 gccgcctgaa ggtcaagaaa tgatctttta ctttatggat catggaactt acagtcgtct
     1081 tggggaaaca acgaaatccc atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga
     1141 cccaggttct tctctattag aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc
     1201 ggcttttgaa aaacgtcctg caaatgagaa agtcgattta gtaaaaatca tgattgccca
     1261 cgaagcaaac cgcacgcagc gaaagatttc tttgacagaa gatgtagcaa aagcattgat
     1321 cggcagtgtg acctatggaa atataggaca attgaagtca aatatccagt tagtatgtgc
     1381 acgaggattt ctaaatcata tgcaatcacc cgaaatctcg atcacaatcg atgatttgac
     1441 agaagggatt cgtagcggat tgattcaatt agcaagcaac cgcagtgcaa tggcagagct
     1501 ttctaaaata ttggagccaa agataactgt agcaccaaat gatacgatca tgaaaatcca
     1561 atcggactcc tatgagttac cttataatct atatgatatc atcggagata aagcagcttt
     1621 actgaagtcg gatggtttgg atcaagaagc aatcaatcat ttcatttcta cagatatcaa
     1681 catccatctg aagtctttct ataaagacca tggtttttct tttaatgcag acagcaaatt
     1741 agcagaattt gtagatccga aaatcataga agtgaccaat cagatttatg taatggtcaa
     1801 aagatcttta ccatatgagt ttcagcaaaa ctttatttat gctatgagct tgcatatcag
     1861 ttcttttttg aaaaaaatca acttaggaga agaacggcat acgaatgata acatccgtga
     1921 gatggctttt gattttccag atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga
     1981 aacgtatttt caagtggaga ttccagaaag tgaagactat tacttgaccg ttctactcgt
     2041 ctcgttacgg gcaaatcaag caagcggcag aatcggtgta gtcgttgcag ctcacggaaa
     2101 cagcacagca agcagtatgg tccaagttgt ccaacagttg ttagatgcag agaacgtacg
     2161 agcagtagac atgcctttgg atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa
     2221 tgtacaagaa gtagatgaag gaagcggggt catactctta gtagatatgg ggtcccttgc
     2281 gtcgtttaat tcgcaaattc aaagagacac tggaattcca gtacgtactg ttgatatggt
     2341 aacaacttct ctagttttgg aaactgtacg aaaggtctca gtattaggga cagatctaga
     2401 tatgttgcat gaatcattga aaaattttcg aggttacgca gaaatatctt ctgtagaatc
     2461 tgttcaggaa gcaaaaccta ttcataagaa aaaagctgtt ttggctatct gcgcatctgg
     2521 cgaaggtaca gcacaaagaa tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga
     2581 aacagaatta gaagttctag ctttgtcgat tgtggaagtg aaagaagaac tgccagttat
     2641 ccaaaaagaa tatcagatca tagctactac tgggattact gatttgaata tccaagcccc
     2701 atttattcct ttagaacgtt ttattgatca aaatatcgaa gtcattttgg atcagttgct
     2761 gatggaatca gaattggagg aaactgaatt catctcccta gatgaagagt cagctaaaaa
     2821 tacgtgtgtt gagtttatct ctgataactt catctttatt aatggtagta agttaatcga
     2881 tccaatgtgg caattttcta cacaaatctc tcagacgacg ggaatcggcg atgaagaata
     2941 tggattcaaa atcaacctag tgatgcacac agcaggaatg atcgaacgta ttattcggaa
     3001 tgaaccattg actgtggaag aaaacgaatt gacaaataca acgaatgatc ctctttatag
     3061 ccaattggca gcctcagttg ttctgttgga agatcagata aaagtaaaag tgccgataga
     3121 agaaatgtat tatctgctac gtctagtcca taatcagtta gataaaaaag aatacactgt
     3181 gccatgaata cacaaaaacg atacactttt atagtgtatc gtttttgtgt attggttaag
     3241 aatcctttaa attcaattag tgtatcgtaa aaaagttggc acgctttttg cttatagtag
     3301 tgtgtgctag gaggtggttg ttcacacaga aatgataggg gtttctgtga agtagtgagg
     3361 aagaaggagt tttgcagatg gatattcgat tggcacgtat agatgatcgt ttaatccacg
     3421 ggcaagtcgc aacagtttgg tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg
     3481 atgtagtcgc tcaagataaa ttgagaaagt ttctactaaa agaagctgca ccgccgggga
     3541 tcacagcaaa tgtaataacc gtgcagaaaa tgattcaaat ctatcatagt ggattattat
     3601 cacgagcaaa agtgatgctt ttattcacca atccccaaga tgttgaagcg gttgtccgct
     3661 gcggtgtaca gttgaagtcg ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa
     3721 tgattaccaa ttttgtttca gtaaatcaac aagatattga ttcctttcgt tacttgaaca
     3781 gtcaagaagt ggagttagag attcgaaaag tacctgctga caggaaaatc cttttgatgg
     3841 aactactagc gaaaatcaaa aaggtgtagc aagaatggtt gttggaaaag gtcaaataat
     3901 tctgttgtaa tggttagcaa ttattaatat ttttgaattt gttacaacaa aagtaaatag
     3961 gaggtataaa tatggtaggg attatcctag caagtcatgg acaatttgct gaaggaatct
     4021 tacaatccgg ttcaatgatc tttggcgaac aagaaaacgt caaagccgtg attttgaaac
     4081 caagtgaagg tccagatgac ttgagagcca aattggaaga agcggttgct tcttttgata
     4141 atcaagacga agtgttattc ttagttgatt tatggggcgg aacaccgttc aatcaatcaa
     4201 acacattatt cgaagagcac aaagataaat gggcaatcgt cagcggtttg aatttaccaa
     4261 tgttgattga agcctacgca tcacgctttt caatggaatc tgctcatgaa attgcagcac
     4321 acatcattga aacagcaaaa gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag
     4381 aagcaccgaa agcagcagtt gaagacgcac aacctaaagg agcacttcca gaaggaactg
     4441 tggtcggaga cggaaaaatc aaatatgtat tagcacgtgt tgactcacgt ctgttacatg
     4501 gacaagttgc gacagcttgg acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag
     4561 atgcagtatc aaaggatgat ctgcgtaaga gactaatcga acaagctgct cctccaggtg
     4621 taaaagcaaa tgttattcct attagcaaaa tgatcgaagt agcaaaagac cctcgttttg
     4681 gaaataccaa agcattgctt ttatttgaaa atcctgaaga cgtattgaca gctgttgaag
     4741 gtggcgtaga catcaaagaa ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg
     4801 ttgtaagtaa agtattgtct atgggacaag aagacgttga agcgtttgag aagcttgaac
     4861 aaaaaggtgt gaaattcgat gtacgtaaag tacctaatga ttcacgcgat aacatggatg
     4921 atattttgaa aaaagcaaaa gctgaattag caaaagcgta ataaataata ttaaaattta
     4981 taggaggctt atcatgtcta ttatttcaat aattttagtc gtattagttg cctttctagc
     5041 tggtatggaa ggtattcttg atgaatttca atttcatcaa ccactagtgg catgtacatt
     5101 gatcggttta gttaccggta acctcgaagc tggtatcgta cttggcggta cgcttcagat
     5161 gatcgcttta ggttgggcaa atatcggtgc cgctgtagca ccagatgctg cattagcatc
     5221 agtagcatca gcaattattt tagttttagg tggacaaggt gttaaaggtg ttccttctgc
     5281 gatcgcgatt gccgttcctt tggctgttgc agggttgttc ttaacaatga tcgtacgtac
     5341 aatcgctgtt ccaatcgttc atttgatgga tgcggctgct gaagaaggaa acatccgtaa
     5401 ggttgaaatg tggcacatca ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc
     5461 agcagcgttg ctatttatcc cagcacaaac tgtgcaatca ttccttgaat caatgccagc
     5521 atggttaaca gatggtatgg ctatcggtgg gggaatggtc gtagctgtag gttacgcttt
     5581 ggtaatcaac atgatggcta caaaagaagt atggccattc ttcgttattg gtttcgttgt
     5641 agctgcaatt tctcaattaa cattgattgc tctaggtgct ttaggtgttg cattagctct
     5701 tatttacttg aacctttcta aaatgggtgg ttcatcaaat aatggaggcg gaagcaactc
     5761 tggtgaccct ttaggcgaca ttttgaatga ctattaatct tgaaggagga aaataaaatg
     5821 gcagaagaaa aaatcaaatt atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc
     5881 ttcattcaag gttcatggaa ctacgaacgt atgcaaaacg gtggctgggc attttctatg
     5941 attccagcaa tcaaaaaatt atacaaaaca aaagaagaac gttctgcagc attaaaacgt
     6001 cacttggaat tctttaatac acacccatac attgcttctc caattttagg ggttacattg
     6061 gctctggaag aagaacgtgc gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt
     6121 aaagtaggta tgatgggtcc tttagctggt gttggtgacc cagtgttctg gtttacagta
     6181 cgtccaatgc taggtgcact aggtgcttca ttagctatgg gaggaaatat ccttggacca
     6241 atcatcttct tcttaggttg gaacattatt cgttgggcat tcatgtggta tacacaagaa
     6301 tttggctaca aagctggttc taaaattact gatgaccttt ctggcggatt gttacaagac
     6361 gtaacaaaag gtgcttcaat tttagggatg ttcgtattag ctgcccttgt acaaagatgg
     6421 gtatcaatca aattcttacc agttgtatca actgtaaaat tagataaagg tgcttacatc
     6481 gaatgggata aattgccttc aggtggtgaa ggaatcaaga gtgcatttga acaagttaat
     6541 aatggcttag ccctttctcc tgaaaaggta acaacattac aagacaactt agaccaattg
     6601 attcctggtt tagctgcatt aggattaaca ttgttatgta tgtggttgct taagaaaaag
     6661 gtttctccaa tcgttatcat ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc
     6721 ggacttttat aatccataaa aatatagctc gggcgttgtc ccgagctttt tttatatagt
     6781 atactgttca taaacaaata tctggaggga gaaaatggtt caatcaatca atacaaaagt
     6841 agacttggta attgatgcta cttcgtttac gggtctgaca gattacgggc aaatcatgat
     6901 aggtgataat ggttttgagt tttaccatgc gcgagataga cgcaaatata tccaaattcc
     6961 atgggaagaa gtggattatg tactagcatc agtcatgcta aagggaaaat ggattcctcg
     7021 atatgccatt caaacgaaaa aaaatggtac atacacattt tcttccaaag aacctaaaaa
     7081 agtgcttcgt gcgatcagag aatatgtaga tgaagataga atggtacaat cattgagttt
     7141 ctttgatgta gtgaaacgtg gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa
     7201 aacttctaag aggaggtttt ctttattttt ttactttgca gaagaaaata tgaaatagca
     7261 aaaccaatta tttgtcccct ttaaatccca tatgctaaaa ttgattagca aagaaggagg
     7321 ttatattatg agttggttat ggacattgat tgttggagcc gtgatcggag caattgcagg
     7381 tgcgatcacc agtaaaggaa attcgatggg ctgtgtaacg aatatcattg ctggactagt
     7441 cggttcaagt attggacaag cgattttcgg cgaatgggga ccaaaagctg caggtatggc
     7501 acttattcca tctattttag gcgcagtgat tttggtagca gttgtctcat ttttctttgg
     7561 aaaacgatca taagttttta caaaatagta aactaaaagg agttgcagcg agactgtgac
     7621 tcctttttgg ctttatttat ttaaaataaa aagaagtttt ccggaaattt ttatttaact
     7681 gtcgtgtacg aagaaaagtt tgctataatg gaaaaaagcg tgcaaagaaa gagggagttt
     7741 cattatgatg ctacgtgtag aaaaattgcg caaaaaaatg caagaagaaa atttggattc
     7801 atttttggtt accagtccgt acaatcttcg ttacttaacg aactttacag gtacgacagg
     7861 attagctgtt attactttgg aaaaagcatt ttttattact gattttcgtt ataccgagca
     7921 ggcggcagcg caggctcaag ggtttgaaat tattaaaaat gtcggaccta tttttgatga
     7981 ggtagcaaac ttagtacgca aagaagaact gcactcatta gcttttgagg aaacaacagt
     8041 ctcattttta gaatattcgg ttttagaaga aatcatcgat gcggaattag tacctgtttc
     8101 acagatgatc gaagaactca gagaagtgaa agatgaagaa gaaatcgcta ttatcgaaaa
     8161 agcatgtcat attgcggata tggcatatga tcacatcctg aaaatgatcc aaccgggcat
     8221 gactgagatt gaagtcgcaa atcaactaga cttttatatg cgttctcttg gcgcttcagg
     8281 tgtttctttt gatacgatcg ttgctagcgg attgcgttcg gctatgccgc atggtgtagc
     8341 aagcaaaaaa atcatcgaac aaggtgactt gatcacattg gactttggtt gctattacga
     8401 agggtacgtt tccgatatga cacgtacatt cgctattggt gatcctgggg aaaaattaaa
     8461 agaaatttat caaatagtct tagatgctca attagcagtt attgatgctg caaaaccagg
     8521 aatgactggt gtccaattag atgcagttgc tagagattat atttcaaaac atggttacgg
     8581 ggaagcattt ggacattcta caggacatgg gatcggttta gagatccacg aaggaccaaa
     8641 cgtttcttct cgtgcagaaa agcaatttgt accaggaaat gtgatcactg atgaaccagg
     8701 gatctactta ccaggaatcg gtggagtacg tattgaggat gacttgttga tcacagaaga
     8761 tggaaatcgt gtgttgactc gttcaccaaa agaattgatc atcttatagt ttcatcaata
     8821 gaagaagtaa gcgtaaaaat cagtgttcac agactttgtt atagaaaatc actcgattta
     8881 atgatacact atagaagaac ttttaagctg taagagataa taaagttggg tgttttctcc
     8941 aattcatagc agtttgcagc tgatcggcgg cagttttctt accacgtgat cactatgccg
     9001 catcatagag aattcggatg tatctcgtgc ttaggcaaag ggaggaataa ataaatggca
     9061 gatgaaaaaa atttgatttt aaacgcaaac caagatttag gagagatcgt gatcgctcct
     9121 gaagtcatcg aagtaattat cgggattgca gcttctaaag tagaaggcgt atacggaatg
     9181 cgcggaactt ttgccagcaa tgtcactgaa ttattaggac gtgcagctca cggaaaaggt
     9241 gtttacttag caaacgagga agatggcttg aaagtagatc tttactgtta tctagaatat
     9301 ggtgtttcag ttcctaaagt agcaatggaa atgcaggaac gggtcagcca gcaagtcctt
     9361 ttcatgacgg atatcgattt atctgaagtc aatatccatg tagtagctgt tgttcctgaa
     9421 aaattagaac aaccaacaat cgaagaactt tttgaagacg aagaggaaga gaatgaataa
     9481 agaattatca agacacgaga ttcgggaaat ggcattgcag gctttatttc cattagactt
     9541 caatgcagac ctaacaaaag aagatgcgat tttcaacgca atcgaattag accaccgcga
     9601 catgatcaac gaagatgaat cagagtttgt tcctgtttat cttgatacgc tagttggcgg
     9661 tgtctgtgca aaaaaagatg aattggataa agtgattgaa aaacatttaa aaaacaattg
     9721 gcacatcaat cggatctcaa aaatggattt ggtgatcttg cgtatcgcga tttttgaaat
     9781 gacttatgtg acagatgtac cagcggctgt tgctttaaat gaagcaatcg agttggcaaa
     9841 aacatttagt gatgaccgtt cacgtaaatt cgtcaacggt gttttatcca atgtattgaa
     9901 agaattagaa gctggggcgt aagtcctagc ttttttctat gaatcatcat aaacctgtta
     9961 taaatcggga aaattctatg gtagaatgaa aacacaaaaa atatctggga gtggttagat
    10021 ggcagaattg attaacggaa aagaattagc tgaaaaaatg caggcagaga ttgctgaaaa
    10081 aatccaaaaa ttgaaagata atggtatcca tcccggatta gtcgttttat tggtagggga
    10141 aaatccagcc agccaaatct atgtaagaaa taaagaacgt gtagcaaaag aaatcggaat
    10201 ccattcattg gtagagcgtt atcctgagac gatttctgaa gacgaactac ttgcagaaat
    10261 tgaaaaatac aatcaagatc ctagttttca tggtatattg gtccaattac cattgccaaa
    10321 gcatatagat gaagaaaaga tcctgttagc tatccgccct gaaaaagacg tagacggttt
    10381 tcatccgatg aatttaggca gactccttgc gggtaaaccg gacaagattc cttgtactcc
    10441 atacggcatc atgaaaatgt ttgaagctta ttctatcgat ccggcgggta aacgagcagt
    10501 agttatcgga cgaagcaata tcgtcggtaa accgatggct cagctactct tgatggcaga
    10561 tgcaactgtt actgttgctc attctaaaac cgaaaatctg gctgaactgg cacaagaagc
    10621 agatatttta gtcgtagcaa taggacgtgg acattttgtc acaaaagaat tcgtaaaacc
    10681 aggtgctgta gtgatagatg tagggatgaa tcgtgatgct aacggtaagc tgatcggtga
    10741 tgtgaaattt gatgaagtgg agcctattgc aggctggatc actcctgtgc caaagggtgt
    10801 agggccaatg actatcacga tgctgatgta tcaaaccgta aaatctgccg aagcttttgg
    10861 agcaggtgaa tagatggcac aagaatattt aactgtcact acattaacga agtatttgaa
    10921 aagaaaattt gatgccgatc cttatttaga acgcgtatat ttaacaggcg aaatatcgaa
    10981 tttccgttta cgtgccaatg cccatcaata tttcagctta aaagatgacc atgcaaaaat
    11041 atctgctatt atgttcaaag gagcattcca aaaattacga ttccagccaa aagaaggaat
    11101 gaaagtcctt gtcgttgggc gtatttcatt atatgaagcg ggaggttctt atcaaatcta
    11161 tgtagagcac atggagcctg atggaatagg tgctttatat caagaattgg aagaacgaaa
    11221 agaaaagctg atgaaggaag gcctttttca agggcctaaa aaaatgctgc cacgtttccc
    11281 aaagcgaatc gcggtcctta ccagtccaag cggagcggtt atccgcgata tcatcacgac
    11341 ggtgaaaaga agatatccaa ttgtccaatt ggttttgttc ccaacagttg tacaaggaaa
    11401 ccaagcagcg gacaatgttg tcaggaatat ccagagagta gaagagatgg gcaatttcga
    11461 tacaatgatc atcggtcgtg ggggtggttc gattgaggat ttatggccgt ttaatgaaga
    11521 gcgggttgct cgggcgattt atcaagctaa tacgccggtg atttcatctg taggacatga
    11581 aacagatacc acgatcgctg atttagtagc agatgttcgg gctgccactc caacagcagc
    11641 tgcagaatta gctgttcctg ttttgtcaga agagttgatg cgcattcaag aacggcaagc
    11701 tagattgcag caggcgtttg tacgccagat ccagcgcaaa caagaacggt tcgagcgtgc
    11761 tcgacgttcc tatgtatttc ggcagcctga aagattgtat gaaggtcaga ccatcaaatt
    11821 agaccagttg aaacagcgat tatatcaagc tacacagcaa atttatcatg cgaaagaaaa
    11881 acaaacgatc gcattaagcc accaattgga acaagtggct ccgctttatc gagttaaagc
    11941 agctcgccaa gaaactgctt atctagaaaa acgcttagaa gagaaaatcc aacaatatat
    12001 gaaacaacag cagcagcgtt ttgctcaagc tgttcaatct cttgatttgt tgagtccatt
    12061 gaaaatcatg ggaagaggct acagctatac gacgttaaat gatcaagtag taaaaagtgt
    12121 caccgagatt catcaagggg atttgctgac gatccattat caagatggaa tcgttaaagg
    12181 acaagtacaa gagatagaca aaggagaata aaaatatgga aaatccaact tttgaagagt
    12241 cactgcaaga attagaaaaa attgtcatgc agttagaaca aggagacgtt ccattagaat
    12301 cagctctaga ttcgtttaaa cgaggaatgg aattaagcaa acattgccaa gatacattag
    12361 ctaaagcaga agagaccttg acgaaaatga tgacagaatc aaatgaagaa gttgattttg
    12421 acggaaacga ggaaagctga gatgagatta actgactttt cagctattca tctacctgct
    12481 gtagaaaaag aaatcttgtc ttttttagat gagcatacaa cggatagagg actctatgat
    12541 gcgatgagtt attcagtgaa agcaggcggg aaaagaatac gtcccttatt acttttaacg
    12601 gccgttgctt cctttgatga gcctattgat gttcccgtct atcaagtagc agctgcttta
    12661 gaaatggtgc atacgtattc cttgatccac gatgatttgc ctgcgatgga caatgatgat
    12721 cttcgtagag gaaaacctac caatcataaa gtattcggcg aagcattagc tatattggcc
    12781 ggagatggtt tgcttacagg agcattccag ctaatcagta tggcacactt aggtaattct
    12841 cctaagcttc tgctgcttca gcaattagct gtttgtgcgg gatcacaagg tatggttgct
    12901 gggcaagctg cagatatcga gggcgaatcg aaaaagcttt ctttagaaga attagcattt
    12961 attcatgaaa gaaagacagg gcatttgatt cgttatgcct tgctggctgg tggcatatta
    13021 gcgaagcaac ctgaagaaat cttattgcta cttcaacgat tagccgaaca tttaggttta
    13081 gcttttcaga taagagatga tctactcgat gtgattggta caacgaaaac attagggaaa
    13141 acagctggta aagatgaacg tatggaaaaa aatacttacc cgcgtttatt aggcctagaa
    13201 aagacacgtg aggcgttgga gattgagctg ttatccgcca acaagatcat agacaaatta
    13261 gaagaggacg ttttttcttt tgatggcggg ctgatgcgcc agatgatcaa gcaatttgat
    13321 gtggagaaat gagggaaaga atggaaaaag aacgcgtaga tgtattagcg gtcaaacaag
    13381 gattatttga aacaagagaa caagccaaac gttcagtgat ggctggtttg atttacaacg
    13441 aaaaaaacga gcggtttgat aaaccgggag aaaaaattcc agtgagtagt gaattaagag
    13501 tcaaaggtaa aaaacttcct tatgtttctc gagggggatt aaaattagaa aaagcactca
    13561 aagcatttga tctatctgtt gaaggaaaaa cattgttaga tattggtgca tccactggcg
    13621 gatttactga tgcagctttg caaaatggcg caaaaatgag ttatgcttta gacgttggct
    13681 acaatcagct agcttggaaa ataagacaag atcctagagt cgtcgtaatg gaaagagtga
    13741 attttcgtta tgcaaaaccc gaagacttta ccttaggaat tccagaagtt gctgttatcg
    13801 atgtttcttt tatttcacta aaactgatgc tgcctccatt acatgctatt ttaaaagaag
    13861 agggagaagt gattgcttta atcaaacccc aattcgaagc aggcagagaa gctgtaggga
    13921 aaaacggaat tgttcgtgac cctcagaccc atcaaaaagt tttagaagac attttgtcat
    13981 ttgcggcaca aggtggatat gatgtgctgg aattaagtta ttctccgatc acggggggag
    14041 aaggaaatat cgagttcttg gctcacttga ggaaagtacc cgaaagcgga acaatcaatt
    14101 cagctattaa tatggcagaa gtggtttcaa atgcacacga acagtttgac cataaataaa
    14161 ttgtaactgt gaaaatcaag tgagaagtat gcggattctt ctttattata ccaaggaaat
    14221 aagctatcat tgattaagaa gaatatgcta aggagcgaag acgtaatgag aaaaaaagat
    14281 cggcatcgtt tgattactcg attgttgagt gagcaagata tccgaaagca ggaagaattt
    14341 gttgaaatcc tgaaaagcaa aggaatatct gttactcaag ctacgatttc cagagatata
    14401 aaggaactga aactgatcaa ggtaccggct tcagatggcg gctaccgtta tagcatacca
    14461 gctgaaacaa acgaagatgt aaatactaaa ttggaaaaac tattaaaaga cgctttcgtg
    14521 gcagttgacc aaatggaaaa atttgttatt ttgaaaacat tgccgggaaa tgcttcggca
    14581 gcagcaaatt tgattgataa acgttacaag aaagaattat tttcgattat caatgatgac
    14641 gataatgttt tgatgattac acgaactgaa gaccaggcag ttttattgaa gaaagacttc
    14701 ctgcattacc tttaagttga ggagcgaatg tgatgctaca agaaatcagt attactaact
    14761 ttgcgattat tccggaattg cggctttctt ttcacgaagg aatgaccgcg ctgaccggag
    14821 agactggtgc gggtaaatcc attattatcg atgctttagg tctgttagca ggaggaagag
    14881 gttcaagcga ttatatacgt caaggagcag aaaaatgtgt gcttgaaggg ttgtttgaac
    14941 taccaaaaca agaaggattc tctgaactga tggttgaatt agggatcgaa acagatgagg
    15001 acaatctgat tgttcgacgg gatatgtcct taactggaaa aaatgtttgt cgagtgaatg
    15061 gtcatattat cacattagct aatctgagaa gaatcggcag ttatttagta gatatccaag
    15121 gacaaaacga acatcaggaa ctgctccagc ctgaatcaca tcttgcttta ttggatcgtt
    15181 ttggagatgt agctttccaa cagaagaaaa agagttatca gcaagaatat atctcttatc
    15241 gtgagctgga aaaaagggta cgaaaagtgc agcaaaatga aaaatcctat gttcagagaa
    15301 tagatatgct ccatttccag caagaagaaa tagctgctgc tgatttgcaa gtcggagaag
    15361 aagagaaatt aaaagaagaa agagaaaaac ttagcaatta tcaaaaaatt gctgatggat
    15421 tagctgcagg ttacggagca ctgacgaata atgaacaaaa cagtgtagat ggtgttggct
    15481 tggccgtttc agaactgcaa gggatcgccc atctagatgt ggagtatgaa gcaatttatg
    15541 aaaatatcca aagtgcttac tatttgctgc aagatgcaat cggcgatatg agtcgtcaga
    15601 ttgatttatt ggagttagat gagagtcgtt tagaagaagt tacgcaacgt ttagaattaa
    15661 tccgacagtt gaaacgtaaa tacggcgaat ccattgagtc aatattggct tactatgacg
    15721 aaatcaccga agagctagct tcttctgatt tttcagaagg tcaattggac aaaatgaaag
    15781 aggaactaga gcaaaaagag cttctacttc agcaacaagc cgcagatctt catgaagcgc
    15841 gtaaaaaaat cgcaaaagaa ttggaaaaat ccattctgca tgaattgaaa agtctgtata
    15901 tggaaaacac tgagtttgag gtacgtttct taaaagaaga aaatagacaa ttgaatcatg
    15961 atggatttga ccaaatcgaa ttttacatta cgaccaatcc aggagagcca ctgaagcctt
    16021 tggtgaaagt cgcttctggt ggcgaactgt ctcggatgtt gcttgctttg aaaactattt
    16081 tttcttctga gcaaggtgtg acaagtatta tttttgatga agtggataca ggtgtgagcg
    16141 gaagagttgc acaggccatc gcagataaaa tttctcaaat atcgaaatac tctcaagttt
    16201 tgtgcatcac tcatctgcct caagttgctg cagtcgccga ctatcaatat tatattgtga
    16261 aagcagtgat tgatggacgt acgcaaactt ctgtttcaga gttgaaaaca aaagaaagag
    16321 aagaagaaat tgcacgtatg ctggctggaa gtgagattac aaaactcaca ctcgaacatg
    16381 caaaagaact attgaaactc gcaaaaagga cctgagccac ctcgcagtgg ttcaggtcct
    16441 tttcagcagc tgatagctat attttatttt tacatgacac tcattacagc agtcaataca
    16501 acagatccaa tcgttaaaat cagcattgtc caaatgacaa attttgttac tttagcaaaa
    16561 gtacttgttt gttttttttc acgcattgtt ttcaacctcg tcattaatta gttgattcaa
    16621 gtgtacaatc ttttttccat ttccacaaga gggctttttt ataaaaactt cccttttttt
    16681 aaataatagg tagttacgat acaaataaat aatgttaata gaactagcca tataaaggca
    16741 tcatcccttt cagcaatagg taacttaatg ttcattccat agatactgcc gacgattgtc
    16801 ggtatagtta aaataatcgt taaagaagtt aaaattttca taatattatt cagattattc
    16861 gatacgattg ctgaaaaagt ctgattcatt tgttctacta gttttaattg aatgcgagaa
    16921 gtagtcattg cctgctctgt ttccaccaat acatcacgta aatgattttt caaagcatga
    16981 ttttcttgga aaagtttatt ttgcgctagt tctttcaatg catctaaatt agcagaagtc
    17041 gcttctctga aataaacgag gcttttttgg atatccatga tttgataaag ttgtttgttt
    17101 tcagtggata cttgaagttt ggcttccaaa gtatcgcatt gagattgaag cgtagctaaa
    17161 taactgttgt aagagagtgt gacctgccaa atgaattgaa agaagatatt gagattaggc
    17221 gaaatatcac tttttggaaa ggtctggcta aagactttct ttaaaaacag tggttcatga
    17281 ttcgtaattg tgataagctt ctttttgggc gtcaaaataa tagagagcgg gtatgtatcc
    17341 acttgaaaat aaccgctagg acttgtttta gcaaaaggat attgtaacaa taataacgct
    17401 ggctttgtca actttgattg attgaattct tctgtacggg aattttctgc atcgtccaat
    17461 atggaagtga tgtaatctga cggaatttca aattgatgtg tcagctgatg aatctcttct
    17521 tgattaggac gttctacaca ataccagatc gtttggtcag tttcctttcc ggtattacgc
    17581 agtttctcat tatctaatgt aaaatatttg atcatgattt tcctcctgtt gcctgtttct
    17641 tttattataa cggataggga attggaatac atggaatgtg tttgtaagat atagaatata
    17701 actgcataaa tttaaataaa atgaaacagt tgtgtaaagt atatacagta tacttgaagc
    17761 aacgataaaa aaggagttta cgttcatggg cacattcaca ccacttatct tgttttggta
    17821 tatttacccg gtaatcgttt tgtttggttg ccaatttctt gtaaaattat tttcgttaaa
    17881 tcgtcgtttt aaaataaaag cccctgattt ggcgattcct tttttgtggg gcggtatgca
    17941 tgcattgtct agaaatacag ggacaatttc tatcttaccg tttttcttta tttctgtttt
    18001 gctaatggga atttgtattg ctgttttcca agcatattat tatgaagaaa ttatttatcc
    18061 gcgttatttc aagatgactt ggcgtttggt ttttctgttg acaatcgtct tgtacgctgt
    18121 cttgatcatt gtgaacatac tttcttacct atgaaaaaaa tagaaaagct gtaaaaagtc
    18181 ttttaaaagg ctttttgcag cttgattttt aaaaaaatgt ttatgaataa aaaaagttgt
    18241 tcccggattt cccagaaaag gtggtagaaa gtgggggatt gtggtagact aattttagtt
    18301 agtggaggaa tggggggctt tcaatgttga tgggtgaatt ccaacataat attgatgcaa
    18361 aaggacgttt aatcgtgccc tcaaaacttc gtgaagaact tggcgaaaag tttgttctga
    18421 cacgcggatt ggatggctgt cttttcggct atccgatgag cgaatgggaa aatctggaag
    18481 ctaaactgaa cgaaatgccg ttggctaaaa aagacgcacg tacttttgtg cgattcttct
    18541 attcggctgc cactgaatgc gaacttgata aacaaggacg aatcaatata cccagcacgt
    18601 taagaaatta tgcagctttg acaaaaggtt gcgtagtcat cggtgtatct aaccgaattg
    18661 aaatttggga tgagacacga tggcaagatt tttctgcagc agcagaagag aactttgacg
    18721 aaattgctga aagtatgatt gatttcggac tataagaata ggaagaggct gactaatgac
    18781 tgaaacattt caacactata cagtcatgtt gaaagaaacg gtagatggat tgaacatcaa
    18841 accggatggc gtttatgtag actgtacact cggcggtgcc ggacatagtg aatatttatt
    18901 aagccaacta agttctaaag ggcatctgta tgcgtttgac caagatcaaa aagcaatcga
    18961 tcacgccaag ctgcgtttag cttcttatgt cgaacaaggt atggtcacat ttatcaaagc
    19021 gaacttcaga gaattagaag aacgcttgag tgagcatgta gataaggtag acggcatttt
    19081 gtatgatcta ggggtttcct ctcctcagct agatgaagca gaacgtggat ttagttatca
    19141 tcaagatgca ccgttggata tgcgcatgga tcagtcagct cctttatccg cttatcacgt
    19201 aattaatgaa tattcctacc atgaattagt aaagattttc ttccgctatg gcgaggagaa
    19261 attttccaaa caaattgctc gtgaaatcga acgagtacga aaaacaaaac caatccaaac
    19321 aactggtgag cttgtcgaaa tcattaaaca agtcatccca gctcccgcaa gaagaaaagg
    19381 cgggcatcct gcaaaaagaa tttttcaagc tgtgcggatt gcagtaaatg acgaactcgg
    19441 tgccgaagag gcctctttag aacaggcaat ccggttattg aaagtcaatg gaagaatcag
    19501 cgtaatcaca ttccattcat tggaagacag aattgtcaaa agtatgttta aagaatatag
    19561 tacggtacaa gatctaccac cgggacttcc tgtcatacct gacgaattcc aaccagaact
    19621 gaaagtcatt actcgtaaac caatccttcc ttcagaggaa gagctaacag aaaacaatcg
    19681 atcacgcagt gcaaagttac gtattgcaga aaaaataaga atgaaagagg agtagaatga
    19741 tggcagaatt aaagaaaatg caagattacg aatatgattt acctgaattg gtggatcagc
    19801 cggaacaaca aatgcctgat tcttcttcga atcaagaaat cattcttcca caatcaccgg
    19861 caaaacgatt aaagcagatt tctgctttgg aaaaaattgc tgtggcaagc gtggcgattg
    19921 ctattgttgc tttatgtgtt ttaacagtta tgctgcgcac aaatatcagc ggagctgaaa
    19981 aagaaatcac gtcaatacaa acagaaatca atgataaaaa gcaagaaaaa acgagtttag
    20041 aacaagaaaa aaacgaactt tctcgtactg aacggattaa acaaatcgca gaagaaaaag
    20101 gattgtctat taatgacgac aatttaagga aagtgaagta aatgagtctt aaaaataaaa
    20161 ttcggcggtt tatggaaaag aaaaatctaa atccgatgaa caatcgcaaa aaagtaggta
    20221 tcattttatt tgctacgagt attggattgt tctttttatt tgccgtccgt ttctcgtata
    20281 tcgttattgg aggtcatgtt gctggtacct cattagctga aaaaacaaaa cagttgtacc
    20341 aaggaagtga agtagtaaaa gcaaaaagag gaacgattta tgatcgtaac ggtgtggcgc
    20401 tcgcagaaga tgcctcctct tattcaatta aagcgatact ctcaaaaaca tatacctctg
    20461 gggataaaaa actgtatgtt gaagaaaaga attttgataa aatagcagaa atcctgcata
    20521 agaatctttc tatagacaaa aaagatgcac tgaatatttt agaggacggc gcaaaaaaag
    20581 agctgtatca agttgaattt ggtagctatg ggaaaaatat ctcccaagaa accaagcaaa
    20641 acatcgaagc ggacatgaaa aaagaaggtg tcgctggtct ttattttgtc gaccatcagg
    20701 ctcggatgta tccaaatggc gtcttttctt ctcattttat tggatacgca gttccagaca
    20761 aagatgagaa tggcttagtt ggtaaactag gtttggagtc tgcttacaat gatatcttga
    20821 gcggtaaaga tggtaagatt atttatcaaa aagataattt tcaaaatcct ctgccaggta
    20881 cagtggcaga agaagaaaaa gctgtcgacg gacaagatat ctacacgact ttagacagca
    20941 gactacaaag ttatttagaa acactgatgg atcaagtaaa tgaagaatat cagccagaag
    21001 aattgacagc agtcctgatg aaagcaaaaa ccggagaaat cttggcaatg ggacaacgac
    21061 ctactttcaa tcctgaaaca atggaaggat tgactggaaa agatgctatc tggcgtaatt
    21121 tcttggtaca agatagttat gaaccaggat caaccatgaa agttttcact actgcagctg
    21181 ctatcgaaga aggagaattc aacgagaatg aaaccttcca atctggaaaa atccaagtag
    21241 aagatgctac gatcaatgac catgactttg gtgaaaaagg tgtgctgacc atgcgtcaag
    21301 ctctttcatg gtcaagtaac gtcgggatgg tcatactgga acagcgatta ggcggacgat
    21361 ggtataacta tttacaaaaa ctgggatttg gacaaagtac tcattctgga ttagatgatg
    21421 aagtaaacgg tgctttacct acattaaata ttgtcgaccg agcaatgagt gcttatggac
    21481 aagcggtagg tgtgacgaat ttccaaatga tgaaagcttt cacttctatt gcgaataacg
    21541 gtacaatgat tcaacctcga tatatcagca aagtggtgga tccccaaact ggagaagagc
    21601 gaacaactca aacagaagtt ctagggcaac ctttttcaaa agaaacaaca gaaaaagtcc
    21661 gtgaatatat gagagatgtt gtcgaaagcg aaaactacgg tagtgtttat ggtgtatata
    21721 gtgtacctgg atacaatgta tcggcaaaaa ccggaacagc ccaaatcgct tctgatacag
    21781 ggggatatca aacaggagat acagcctacc tgtattcgat tgtggagatg gttccttctg
    21841 aagatccaga ctatgtcttg tatctaacaa tgaaacaccc taagacatat gatcgaatgg
    21901 cattggctaa aatcgcaaat ccattgatga aacgggcaat ggattttaaa gaaactgaag
    21961 aagattcaga tacagaaaca aaaacagaaa aagtatccgt agccgattat cgtaatcttg
    22021 aagcggacgt tgcagcagct gatgctcaaa aaagtggact gcaacctgta gtgatcggta
    22081 atggtaaaaa agtacaaaaa caatctactg ccaacggtga tcagttgatc tctggagaaa
    22141 aactgatcct atatactggt ggtgataagc tgatgcctga cgtaacagga tggtcaaaag
    22201 cagatatcat gaagctgggg aaaatcctcg gtattgaagt tagttttgat ggagatggtt
    22261 attgtgtcaa acaagaactt gctccatatg aaaaaatcac tgaagacaaa cttagtttta
    22321 ctttagaaga ataagaagag aaataggaga gaaaagagca tggaatggac acaagcattg
    22381 attcccattg tcagcagttg tgcaatgaca attgccgcaa tgccgctatt tattggttat
    22441 tttcaaatga aaaagcaagg tcaggcgatt cgtgaggagg gaccaaagtg gcacaatagc
    22501 aaagccggaa ctcctacaat gggcggctta gtgtttctga tcggctctat tttgacagga
    22561 atttgggttg gagcttggca aaaacagcta actcctactc ttttcatctt gctttttgta
    22621 ttagcgttat acggagtaat cggctttctt gatgatttta taaaaatatt caagaaaaga
    22681 aacatgggac tgaactcgaa gcagaaattg attggacaga ttctaggggg aatcatcttt
    22741 tatcttgttt accgttctga aggttatcca ggtgtattga atttcttcgg actagatcta
    22801 cctttaggaa tcatttatgg tattttcgct ctcttctggc ttgttggatt ttcaaatgca
    22861 gtaaatctga cagatggcat cgatggatta gttgctggct taggaagtat ctcttttgca
    22921 gcttacggtc taattgcttg gcatcagcat cagtatgatg tattaatcat ttgcttaagt
    22981 gttttaggag gtttactggg tttcttcgtc tacaacagaa aacctgccaa gatttttatg
    23041 ggagatgtcg gttcattagc gttgggcgga cttttagcgg caatttctat aatgctaaac
    23101 caagaatgga cattgctttt gattggtttg atctatgtta tggaaacagc aagtgtgatg
    23161 cttcaagtta cttcctttaa gcttacagga aaacgaattt ttaaaatgtc tccaattcat
    23221 catcactttg aaatgtgcgg atggtcggag tggaagattg atacagtttt ttggttgatc
    23281 agcattgtga cttcgttgat cacattatgg atcgtttggt aggagatgac gggaaatgaa
    23341 aaaaatatca acatatgaaa ataagaaagt gcttgtctta ggactcgcaa aaagcggagt
    23401 aagtgcggcc aaattattac atgaattggg cgcacttgtc accgttaatg atggaaaacc
    23461 ttttgacgaa aatccggaag cacaagagct gctttcttta gggataaaag taataacagg
    23521 tagtcatcca atcgaattgt tggatgaaga attttcattg atggtcaaaa atccagggat
    23581 tccctacagt catcctttag tagcaaaagc ccaagaaatg ggtattccgg taattacgga
    23641 agttgagcta gcttacgaag tagcggaatg cccaattatt gggattacag gaacgaacgg
    23701 aaaaacaacg acaacaacga tgacaggact actactgaat gcaggagcag atcaaggaat
    23761 tgcacgttta gcgggtaata ttgggtatcc agcaagtggt gtagcgcaag aagcaaaatc
    23821 agaagataaa attgtgatgg aattgtctag cttccaactc atgggaatca cagatttccg
    23881 ccctcatata gcggtgatta ccaatatcta tgaagcacat attgattatc atggtacaag
    23941 aaaagaatat gtcaaagcta aatggaacct tcaaaaaaac atgacagaaa aagattatct
    24001 gatccttaac tggaatcagt cagaactaca agaattagcg caaagaacaa aagcacgcgt
    24061 actgcctttc tcgacaaaag aagtgttgga agacggtgtg tatgctgacg attactcaat
    24121 atactataaa aaagaaaaaa taatggaaat atctgaactt ggcgtaccag gtaagcataa
    24181 tgtagaaaat gcactagctg ctatttctgt agcaaaattg tatggtatat caaatgaagc
    24241 tatacgagaa acgttgcatt ttttccatgg cgtaccacat cgtacgcaat atgtaggaga
    24301 aatccaaggc agaaaatttt ataatgattc aaaagcaacc aatattttag cgactaaaat
    24361 ggctctaagt ggatttgaaa cgtctaaggt cgtgttattg gctggcggat tagacagagg
    24421 aaatacattt gatgaattaa ttccttcatt aaaaggaatc aaagcaatgg tcgtatttgg
    24481 ccaaacaaaa gaaaagttga tggatgctgg aaaaaaagca ggcatcgaga caatagtaac
    24541 agccgattct gtagaacaag cagttccttt agcattagaa aacagtacag atggtgatgt
    24601 agttttatta tcaccagcga atgccagctg ggatcaatac ccaaattttg aaactagagg
    24661 aaacagattc atggaagctg tgaaccgatt aaaatagaaa aagtagaagc gagcgataaa
    24721 aaatgaaaat tttggttaca ggcggaggaa cagggggaca tatttatcca gctcttgcct
    24781 ttgtcaatta tgtaaagact caagaaccga atgctgaatt catgtatgtg ggagcaaaac
    24841 gaggtctaga aaacaaaatc gtccctgata caggaatgcc tttccatacc ttggagattc
    24901 aaggatttaa acgaaaaatt tccatgcata acatcaagac ggtccgattg tttcttaaga
    24961 gtatccgtca agcaaaaaaa atcctcagtg attttcaacc agatatcgtt atcggtactg
    25021 gaggatatgt ttccggagcg gtagtgtacg ctgcagccaa aatgggtatt ccaacagtta
    25081 tccatgaaca aaacagtgta ccaggtgtta ccaataaatt tttaagccga tatgtggatc
    25141 ggatcgctct ttcatttaaa gatgcggctt ccttttttcc tgaaaacaaa gcagttcttg
    25201 taggtaatcc tcgagcacaa gaagtagcag atactaaaaa atcagatatt ttgaaaacat
    25261 ttgatttgga tccagaaaag aaaacagtct tgatttttgg cggtagtcaa ggagcactta
    25321 aaatcaatca agcagtatca gtttttttaa gtgaatatat tccttcttct taccaagttt
    25381 tgtatgcttc aggagataga tattatgaag agatcctaaa taaaataaat cctgaaatac
    25441 aaaatattag tgtccagcct tatatcaaaa atatggcaga ggtcatggct tccagtgatc
    25501 tgctggtggg gcgtgctggt gcaacttcga tcgcagaatt cacagctctg gggttaccgg
    25561 cagtgttggt tcccagccca tatgttacga atgatcatca gactaaaaat gcaatgagtc
    25621 ttgttcacgc aggagcagca aaaatgattg cagataatga actaacaggt gaatcactaa
    25681 gtcagacagt caacgaaatc atgggtgatg aagaattgca aaagcaaatg tgccgagctt
    25741 ctaaagaaca aggaatccct gatgcttcaa aacgtcttta tgacttagta aaacaaataa
    25801 tcaaaaaata aaggcggtgg tcttagtgag taagaaaaaa gaaccaaaaa aattgtctca
    25861 tccgtcttct gatgaaaaca atttgactcc ttggcaaaag gctaaccgaa aatatctggc
    25921 agaacaagag atgaaaaacc aagagccaaa aaaagaagac ataacattag atacacccct
    25981 ttcagattct gaagaagaaa aaacggttca agaggaacag caagaagaaa gccagtccga
    26041 gaaaaaaatc gaattttttg aagagatcaa gcaaaagaaa aacggtggac catataacgg
    26101 ttcttttttg aaccggctgc ccaatttaaa aagtcaacgg cacaaagtac tatttcgtcg
    26161 gttagctttg atcatcactg tattgggtat accgctgatt tttttgatct attatgtttc
    26221 tccttatagc aaattacaag cagttgcagt atcaggcaat aagacagtca acagccaaga
    26281 aatcatctct gatacaaaat tgtcattagg agaaaatgtt tggggacagt attttcatcg
    26341 atctacttat atcgatcgcc ttaaaaaagc acagccaaga atcgaaacag caaatataca
    26401 tttcaaaggg atgaatgaat tcgatttgga tgtaacagaa tataaagaga ttgctttgat
    26461 cgcaaaaaac aatcaatact acccagtgat tgaaaatgga acagtattag atgaaaaagt
    26521 agcaaatcct acaaaaaatc tccctatctt ggaagagttc aaagactctg caaaaatcaa
    26581 agaattaaca aagcaataca atcagttatc atcagaatta caaaaagcaa tttctgaaat
    26641 caagtacacg ccaagagcaa gcaataaaaa tctgatccaa ttgaatatga atgacggcaa
    26701 tcaagttatt gtaaatatca ataatcttgc gaaccaaatg aaatattatt cgcaagtagc
    26761 caaagatatg gatgaaaaag gtgtgatcga catggaagtt ggtatcttct cgcatcctta
    26821 cggtagcact caagaaacgg aagaatcggg ccaatcggaa gaaacaactg aaagtgaaga
    26881 aaatgcggat caaacaaacg aatcaagtga agaggcagaa acgcaaaaag cacaaacacc
    26941 tggatcgtct agcacaaacg gatcatctga ttcaaatgat tcaagtgaaa caagtcaaat
    27001 agaagataat ccaagtacaa ctccgtcaag tttaaatgaa aattgataaa acaactttaa
    27061 aaagtaagaa tgtactttct aaaaaccagt aatttatggt agaattggaa gcagtgaata
    27121 tatctattaa taagtatatt ttaaaacgga cgctagttgt tcgcttgtat atggaatagt
    27181 aagtaggagg gaatcccatt catggcaaaa acaggaatgt atgtaggcct tgatattgga
    27241 acgacatctg ttaaagttgt cgtggctgaa tatatcgaca gccaaatgaa tattattgga
    27301 gtaggaaacg caaaatcaga agggattaac cgaggcatta tcgttgatat cgataaaacg
    27361 gtccaagcga tacaacgagc agtacgacaa gcagaagaaa aagcaggtat ccagatcaaa
    27421 ggcgtaagtg tcggtttgcc agcaaacatg cttgaagtag aaaattgcca gggaatgatt
    27481 gcagttaacg gagattcaaa ggaaatcacc gatgaagatg tcagaaatgt ggcttcagct
    27541 gcccttgttc gttcaatacc tcctgaacgt caaatcgtgt caatattgcc acaagatttt
    27601 actgtcgatg ggttcgaggg aatcaaagat cctcgtggaa tgatcggcgt aagattagaa
    27661 atgtacggtc tgttgtttac aggacctaaa acgatcatcc ataacatacg taaatgcgtg
    27721 gaaaatgccg gattaatcgt caacgaaatg gtgattactc ctttagcgct gactgaatca
    27781 attctttctg acggagaaaa agattttggt acgatcgtca ttgatatggg tggcggccaa
    27841 acaacaacgg ctgttatgca tgataagcaa ttgaagttta ccaatcttga ccaagaaggt
    27901 ggcgaatttg tcactaaaga tatctctatt gtattaaata cgtcatttaa caatgcagaa
    27961 gcgttgaaaa taaactacgg ggatgcatac ccagaacgga catcaccaga tgaagaattt
    28021 ccagtagatg tgattggtca gtcagaacca gtgaaagtcg atgaacgcta cttgtcagaa
    28081 attatttcag ctcgaatgga acagattttc aacaaagcaa aagatgcatt ggaccaaatc
    28141 gaagcattag agttgccagg tggcgtcgta ttgacaggcg gagctgctag tcttcctggt
    28201 gttgtggact tggcacaaga aatcttcggc gtaaatataa aattgtatgt tccaaatcac
    28261 atggggctga gaaacccggt atttacaaat gtaataagta tcgtggatta ttcagcaaac
    28321 ttgagtgaag tgtatcaatt ggcaaaaatt gctgttacgg gtgaaacctc agcagctcgt
    28381 caaatggtag ttgaacaaga aacaacaaac acatatgaga gctatgaagc acctgaagaa
    28441 acaatctacg atgagccgga accaaaagag tccggagaaa atgtaaaaaa taaaatcaag
    28501 ggtttcttta caaatatttt tgattaattc atcctgggag gaataagata tggaattctc
    28561 aattgataat aatatcaatg acggcgcagt aatcaaagtc atcggagtcg gtggtggcgg
    28621 tggaaatgct gtaaaccgaa tgattgaaga aaacgttaaa ggtgttgaat tcattacagc
    28681 caacacagac gtgcaagcat taaaaaattc aaaagctgaa acagttatcc aattaggccc
    28741 caaatatact cgtggtttag gtgctggttc tcagccagaa gttggacaaa aagcggcaga
    28801 agaaagtgaa caatccttac gcgaagcttt agacggtgca gatatgattt tcatcactgc
    28861 aggaatgggt ggaggaaccg gtacaggtgc tgcaccaatc gttgcaggta tcgcgaaaga
    28921 attgggcgca ttgactgttg gtgtcgtgac acgtccattc acttttgaag gaccgaaacg
    28981 tggccgtttt gctgcagaag gtatcgctcg tttgaaagaa aatgtggata cattattaat
    29041 tatctcaaat aaccgtttat tagaagttgt agataaaaag acaccgatgc ttgaagcttt
    29101 ccgtgaagca gataatgttt tacgacaagg tgtacaaggt atctctgatt tgatcacagc
    29161 gccaggttac gttaaccttg actttgctga cgtgaaaaca gtgatggaaa accaaggtac
    29221 agcattgatg gggatcggtg tggctagtgg agaagatcgc gtagtagaag caacgaaaaa
    29281 agcaatctcc tctccattgc ttgaaacatc tattgatgga gctgaacaag tacttcttaa
    29341 catcacaggc ggtttggata tgacattgtt cgaagcacaa gatgcttcag atatcgtagc
    29401 aaatgcagca actggcgatg taaatatcat tctaggtaca tctatcaatg aagaaatggg
    29461 cgatgaaatc cgtgtgacgg tcatcgcaac aggtatcgat gaatcaaaaa aggaacgcaa
    29521 gtctagccga ccagctagac aagcacaaat gcaatcacct gctcaaaaga ctgtattaga
    29581 tatggatcaa gctaaaccaa cttcttcaga agaagaaaac agttttggcg attgggatat
    29641 cagacgtgaa caaaatgttc gtcctagagt tgatgattcc aattttgaca atatcgaaaa
    29701 aaaagaattt gatacattca accgcgaaga aacaaaatct catggcgatg atgaattaaa
    29761 tacgccaccg tttttccgtc gtaaaaaata aaggagagga agagcacatg attgctgaca
    29821 acttgcagaa aatcaagcaa gaaattcagg attcgtgtgc tcttgttcat cgtgatccga
    29881 aagaagttac attagtagct gtaacaaaat ctgttgaatc cgctcaagct gaagaattgg
    29941 ttagagctgg cgttatgaat tgtgcagaaa accgagtaga taaattatta gaaaagaaac
    30001 aagatttatt tgattttcct gaaataaagt ggcatttaat tggtaattta cagcgcagaa
    30061 aggtaaaatt gattgtaaat gaaattgatt actttcatgc gttggacagt ttaagattag
    30121 cagaagaaat ccagaagcgg ttagagaaag aattatcctg ttttattgaa gtaaatgttt
    30181 ctggtgagga atcaaaacat ggaatttcac cagatgagct tttaccgttt gtggaatctt
    30241 tagctgcctg cacaaaaatc aaggttgttg ggttgatgac tatggcgcct aaagaagctt
    30301 ctgatgagca aatccataaa gtgtttggta cgctgaaaga attgcgtgat cttgttcaag
    30361 caaaaggtta tgcccatgca ccatgtagtg aattaagtat ggggatgagt caagactttc
    30421 aaattgcgat tgaagaaggt gcaacattta tccgtgtagg aacagcgcta ttcaaagacg
    30481 aataaaggag gaacaaaaat gccgctttta aacagaggag caattgctaa ttttttcgga
    30541 ttagctgatg aggaagagta cgaataccaa gattatcctg aaacaacaaa aaaggctgta
    30601 cagccaacta aaaaaaatac aacacagaat caatcaactc attctgtatc tgctgcgaat
    30661 actcatgaaa aacaaaaaac ggcgacttca cgtcctgtaa cgcatgaaag tattcagccg
    30721 gctgaaaaga aaaaagtaat tgaactacat caatcaccta caaaaactgt agcgaaagaa
    30781 agaacagctt caagaacctc tacacaacct aaagcaaatc ttgctggaaa aatcactgta
    30841 atggaaccaa gagcttattc tgaagctatg gcgattgcta aacgaatcat tgctggagaa
    30901 gcaaccttag tgaattttca tttagtagaa gaaaaacaag cgagaagaat cgttgatttt
    30961 ctaacaggaa cagtttatgc actagatgga gatatcaaac gtgttggaga tgagattttt
    31021 ctttgtacac cgactgggat ggagattgac agttcaaccg ctcaatcttt tgcagatgcg
    31081 aatttgtttg atctgtaaga ggaggaacta cgatttatac tattgcacta ttacttcatt
    31141 tacttcaacg ggcagcctat ttctatagca ttatcttgat ggtatatgca ttgctctctt
    31201 ggtttccagg cgggtatcaa tcagggctag gacgattatt agctaagatc tgcgaaccgt
    31261 atatcagttt gttcgatcgt ctgcctttga ggattggacc cttggatttc acaattttag
    31321 gggcaatcct gatcttgaac ttagccgtac aaggtttagc acagatcgtt atatggataa
    31381 ctgtccattt agtttattga ttgaagcaac aaaatcttta ttctcagaat agataggagg
    31441 ggaaaatttt ggatgcaaat gtataccagc actttcgggc agatgaacgt acatttatcg
    31501 attcggtagg cgattggatt gaacaggttc aaggacaata tgcaccctat ctgaccgaat
    31561 ttcttgaccc aagacaatcc tatattttag aaacactgat tcgacaaagc agtgatttgc
    31621 gtttcatgtt ttatggcgga tatgaacagg cagaaagaaa aagatgtttg attttccccg
    31681 attattacga acctgtagag gaagattttg aaacagcatt gtatgaaatc cattatccta
    31741 gcaaatttgc cagcctaagt catgggaaaa tccttggaac tttagtggga acggggatca
    31801 aacgctctta ctttggcgat atcatctcgg acggtgatcg ctggcaagta tttattgcaa
    31861 atgaggtgga tcattttgtt gctgcacaag taacaaagat tggaaaggtt gctgtacgct
    31921 tggaaccaat caaatacaca gaaatgatta tgccgaaaga tagctggaca caagaaagag
    31981 gaacagttag ttccatgcga ctagacagtg tgatctctga gatttataat atttccagac
    32041 agcggtccaa gcagctgatc gaagctggta aagtaaaagt gaactggaca gaaaatacaa
    32101 gacctgattt tgtacttgaa ctattagata ttatttcagt cagaggttat ggcagagtcc
    32161 agatccaaga acttgagggg aagacgaaaa aagaaaagta cagggtactg ttcggtgtat
    32221 tacgtaaata aaataaaaag gtggtttact catggcttta actccattag atatccaaaa
    32281 taagacattc ccgacaaaaa tgcggggata taatcaagac gaagtagatg atttcttaga
    32341 tttagttgtt cgtgactatg aggaattgac tcaaagaaat cgagaattag aaaaagcagt
    32401 caaacattct gaagaaaaac ttgagtactt caatgaatta aaagatgctt tgaaccaatc
    32461 aatcatcgta gctcaagata cggctgataa agtaaaaaca agtgcaagca aggaatccga
    32521 agtgatcgtt acttctgcac aaaacaaagc agatgagtta gttgcgaacg ctgaaaaacg
    32581 tgcacatcaa ttaacaactg atgcagaaga aaaagcaaga aaaatcttga cagatgcaac
    32641 agaaaaagca agacagcttg cgacagaaac tgaagatctt aagaaaaaaa cacgagtatt
    32701 ccatcaacgt atcagcttga tgcttgagtc tcaattagaa caagtgaaaa gtccggaatg
    32761 ggatgaaatt ttacaaccat tctccagcta cgtgactgac agccatgaag tgatcaaaga
    32821 agttttagca aaacagcttg acaatgaaaa tgatactgat gtaaactcag acacaggagt
    32881 catagaagca cctatgacgg catttgatac acaggctatt gatatctcga tcgtgcctgg
    32941 tgaaaatgaa aacgagtaga acagaaaagc actttgttat tcttaaagcg agccgatgag
    33001 agtggaagtt cggtaagaca ctgcatggtg aaagatcctc tacttgagac ctgcttttcc
    33061 gaaattagag taagaagcag cggctgacct cgttattggt cacagagagt ttgcactaga
    33121 tagatgcaac taaatttggg tggtaccacg gcatgttcgt cccttgcggc tgaacatgct
    33181 tttttgtgtc cggaaaacaa ataaaggagt gtatggttga atgaaaatga aagaaacctt
    33241 gcaattagga aaaacaaaat tccctatgcg tggtaatttg ccaaaccgag aaggcgagtg
    33301 gcaaaaagaa tgggaagaaa atcgaatcta ccaaaaaaga caagagttga acgaaggaaa
    33361 acctagcttt gttcttcatg atggacctcc atatgccaat ggtaatatcc atttaggaca
    33421 ttcattgaat aaaatcagta aagacattat cattcgttca aaatcaatgt ctggctatcg
    33481 tgcaccatat gttccaggat gggatacaca tggattgccg attgaacaag tattggctaa
    33541 taaaggcgtg aaacgtaaag aaatgtccat ggcagaatac ttggaaaaat gtcgtgaata
    33601 tgcactttcc caagtagata agcaacgagc agatttcaaa cgcctaggag ttgcaggaga
    33661 ttgggaacat ccttatgtta cattagatcc ttcttatgaa gctgcacaag tacgcgtatt
    33721 tggtaaaatg gctgaaaaaa gctatattta caaaggattg aaaccaattt actggtctcc
    33781 gtcaagtgag tcttctttag cggaagctga aattgaatat aaagatgtaa aatctccatc
    33841 tatttttgta gctttcaatg tcaaagacgg caaagatatt cttgatgaag atacagcttt
    33901 tgtcatttgg acgacaacac catggacatt gccagcaaac caaggtattg ctgtaaatcc
    33961 aaaatttacg tatgtattgg tggaagccga tggccgtaag tttgtcgtag cgaaagattt
    34021 gctagaaaca gtccaatcag aaatcggttg ggaaaatgta acagttctaa aagaatttgc
    34081 tggacaagaa atggaatata tgactgcaag tcatccattc tatgaacgtg aatcacttgt
    34141 gattttaggc gatcatgtta ctttagacgc tggtactgga ttagtccata ctgctccagg
    34201 acatggggaa gatgactata tcgctggtag caaatataaa ctatctgtag tgtcacctgt
    34261 agactcaaaa ggattgttta cagaagaagc acctggattt gaaggtatct tttatgacaa
    34321 agcaaatcct atgatcactg atctattaaa agaaaaagga gctttgctga agctagattt
    34381 ctttactcat agttatcctc acgattggcg gacgaaaaag cctgtgatct atcgggcaac
    34441 tccacaatgg tttgcatcta tcgataaatt ccgtcagaat attttggatg aagtagaaaa
    34501 agtagagtgg gttatcccat ggggtaaaac acgtttgtat aatatgatcc gtgaccgcgg
    34561 cgattgggta atttcccgtc agcgtgcatg gggagtacca cttccgattt tctatgctga
    34621 aaatggagaa gcaatcatca caccagaaac aatcgaacat gtggcaaaat tatttgctga
    34681 acatggatca attatctggt ttgaaaaaga agcgaaagaa cttcttcctg aaggatttac
    34741 ccacccaggt tctcctaatg gcgaatttac aaaagaaaaa gatatcatgg atgtctggtt
    34801 tgattctggt tcttctcatg aagctgtttt acgtcagcga ccagaattat cattcccagc
    34861 agatatgtat ttagaaggat cagaccaata tcgtggctgg ttcaactcaa gtatcacgac
    34921 tagtgtggct atcaatggtg tagcaccata caaatcagtt atttctcaag gatttgcttt
    34981 agatggtgaa ggccgtaaga tgagtaaatc tttaggaaac atcattgcac cagaaaaagt
    35041 gatcaaacaa tttggtgcag atatccttcg cttatgggta tctagtgtag attatgaagc
    35101 agatgttcgt gtttctatgg atatcttaag tcaagtggca gaagtatatc gtaaaatcag
    35161 aaatacgatg cgtttcttat tagcgaatac agaagatttc gatcctaaaa aagatactgt
    35221 ttcgtacaat gatttacgtt ctgtagacaa atatatgacc gttcgattga accaagtaat
    35281 caaagaaatc cgagaagaag ggtacgacaa atataacttc atgcatattt accgtacagt
    35341 tatgaacttc ttgacggttg atctatcgtc attctacctg gattttgcta aagatgtagt
    35401 ttatattgaa gcagaagatg attatcaacg tcgttgcatg caaactgttt tctatcaaac
    35461 agctgtagct ttgacaaaat tattgactcc tatcattccg catacagcag aagaaatctg
    35521 gtcattcctg aaagaagaag aggagtacgt tcagttaagc gagtttcctg gttatgaaga
    35581 gtttgcaaac caagaagaat tgatggatac ctggactgct ttcatggact tccgtgataa
    35641 tgtcttaaaa gcattggaag aagcaagaaa cagcaaattg attggtaaat ctttggaagc
    35701 aaaactaact gtttatccaa aacaacaagt tcgtgaaatg ttaaaagctt tggatgcaga
    35761 tattgcacaa ttattgatcg tttcaccaga ctactttgaa gttatggcag cagatgaaca
    35821 agtgccagat aacgctatgg tatttgatga tgttgctatt acagtggaaa aagcagacgg
    35881 ggaaacttgc gatcgctgtc gccaagttcg taaagatgta ggagtagatg aaaaacttcc
    35941 tcatctatgc ggtcgttgtg caaaaatcgt tgaagacttc tatcctgaag ctgtagctga
    36001 aggattcgaa gaaaagtaaa agggaaaagg gggtgtgaca tgttctttgt cacaccccct
    36061 tttgttatca atcgtttaat tttccacgtt cacgatacca gttatctggt tgccagatcc
    36121 attcgaatcc gtctttttca agtagttcga aagctgcttt aggtcccatt gatccagctt
    36181 tgtagttggg aaaatccggt tgtgtttggt cccaagcttt tcgaatgaca tccacgatat
    36241 gccaagattg agcgacttct tcccaatgag agaagttcgt tccatcacca ttcaacgcat
    36301 ctaatagtaa tttttcgtaa gcttctgggc tattttcaac catttctgca ctatttctaa
    36361 attccaattt gattggttcc gtttcaaatc cttgccctac ttctttccca ttcaaggata
    36421 aagaaaatcc ttcagttggc tgaatataga tcgttagaac attaggtgga agtgtcgtat
    36481 cgtcacatgg ttcatcgaca gatgttttga atacattaac aggaacttgt ttgaatacga
    36541 tgttgatccg agtccctttt tcagtcaaac gtttgcctgt acgtacatag aaaggaacac
    36601 ctgaccaacg gaagttatcg atagtgaatt tgcctgcgac gaatgtttca gtctctgatt
    36661 gagcatccac tttgtcttct tcgcgatagc ccttgaattg tacaccatcc aattcacctt
    36721 gagcatattg cccgcgaacg aagttttcta acacttcttc ttcgttataa atgcggatag
    36781 ctttcaatgc tttgatcttt tctgtacgga tttctttttc ggaaaatgct actggcggtt
    36841 ccattgcaag taatgctaca acctgtaaaa tatgattttg caccatgtct ttaagtgcgc
    36901 cactatgatc gtagtagccc ccgcgatctt ctacaccgag gttttcgcca aaagtaattt
    36961 ggatattgtc gatatagcga ttgttccatt gagcctcaaa aatattattg gcaaaacgga
    37021 tagctgagat gttttggatc atttcttttc ctaaataatg atcaatcctg aaaatatctt
    37081 gttctgggaa tacttctctg atttgctggt tcaattcaaa agcagactgg taatcagaac
    37141 cgaacggttt ttctatgatc aaacgattga agccattttt tgtcataata ttttgtgatt
    37201 tcaaatgaga aacgatcgtt ccaaaaaatt gaggtgccat agccaaataa tagacacggt
    37261 taccgtttaa ttgatagtgt ccatctaatc ggtcagacaa ttcttttaat gtctgataat
    37321 gcgttgaatc atttacatca tgggattgat agtaaaaatg gcttgcaaag gtagttgctt
    37381 cttcatctga aggattcaat gcacggattg tttctttgac aacttcacgg tagtgctcat
    37441 cgctccaggg acggcgagcg gttccgatta cagcaaaatg ctcgcctaaa tttccttttc
    37501 tgtaaaggcg gaacaaagac gggtacaatt tacgctgggc taggtcacct gtaccgccga
    37561 atatcgtaaa caatacattt ttttcattag acatgtggat acactccttt atattttatc
    37621 gacaaaaatc gtgctggccg ccttaaagct gacggggatt ttctttctat cattttggat
    37681 agtgatcggt ccttcataag ctgcgatgtc aacgatctca tattcttcgt tgatattcag
    37741 gtcaatagtg actaagtaat ccaacaattc tttttcatca agcactcgtg cgatacgcac
    37801 ttttgtaccg acaggatagg actccaatgt ttgtcggcgt tcttcatgga caacttcctc
    37861 ttttttagga atcgatcctc catgaggaca atgttcagga tgattcaaat agttttctaa
    37921 atgatctgca agatgttccg aagtcacatg ttccaatact tcagcatcat catggacttc
    37981 attccagcta tagtctaaat gttcaactaa aaaaacttcc cataagcgat gtttgcgaat
    38041 cagactgcta gctttttgca gtccgcttgc agtcaattgt acaccttgat aaggggaatg
    38101 ttctacaagc tcttctttca caagtttaga aatcatttca ctgacagatg cagctgagac
    38161 atctagacta gatacgattt gtttattgtt gactttcgtt gtatctccac ctagttctaa
    38221 aatgattttc aaataatctt ctcgatttgg tgtcatattc gatcatcctt tcatggtaaa
    38281 aaaccatcat tagtaagtgt aacaaaaaaa acgttaattg actatttttc taaataaaaa
    38341 acaaaggatt tttagacgtt tttacaacaa aaaacgaaag atgatagaga agactcattc
    38401 tttcgtttat aaaggatata tatatacatt gggagggatc tatttttcag ataacaactt
    38461 atatgaagtg tctgcagatt cgtcataaag agtttgttcg ggataagaaa agataagata
    38521 aaaaccatac tgatcacgta cgactaaacg atattcatct atttctacca cagatgtgcc
    38581 aagagactga ccgttgatca aaaacgtgta atcagatgta atctgtaatt gttgtttttg
    38641 ctggctatca ctaaagaacc atgtaccttg atactttgag agtccttctg acttttccgc
    38701 ttgttttttc tttttcaagt aaataaaact tgagagaacg gtaatgagtg aaccaagaaa
    38761 atagatcatt ttttttatca ccaaatcact cctttatgag aaaataatcg catagacata
    38821 ttctttcttc tttatttcat tgtaccgaat aaaagctaaa atttctctaa gactttcgac
    38881 tggttttttt actgtttttc tacaaattat gataggatac atagtaggaa gaaaggtgac
    38941 ataatttgaa gtatcagcaa gtggaaatag ctttttttat tgaccgcccg aatcggttta
    39001 tagcacattg tatgaaaaaa gacggagaaa taattattgc gcatgtcaaa aatacaggta
    39061 gaggaaaaga ggtcctgata ccaggcgctg aagtggctat tgtatttgct cctggaccta
    39121 aacggaaaac cgcctatgat ctgattgctg taaaaaagaa gggtgactgg tttaatatcg
    39181 atagtcaatt gcctaatcgg ctggcaattg atggtatttt agacggcacg atccttttac
    39241 caggattaac tggtccattt acggtataca agcgtgaagt aacttatctt cactccaaat
    39301 ttgatattta tttagagacg gtggtcggac aaaaagcatt tgttgaagtc aaaggtatga
    39361 cacttgaaaa taaagcgatt ggtgcttttc ctgatgcgcc cacactcaga ggattgaaac
    39421 atataggaga attaacgtat gccgcacaag atggttatgc cgcttatgtc ttgtttattg
    39481 ctcagttcga acatcttcat ctggcaacga tccatgaaga aatgcaacca gcactagctg
    39541 atatggttcg acatgcgcag caaagcggag ttcaaatcct cgcctataat tgtcaagtat
    39601 cggcagatga ggtaactgtc aagcaggcca ttccatttga tgtagatatc ccttttgaag
    39661 atcctataca atagaaagaa ggtaaaataa tgatcaactt aggtattatc ggaacaaatt
    39721 ggattactca tcaatttgtc caagcagctt taagcacaaa aaaatatgaa ttgacagcgg
    39781 tatattctag acatttggaa aaagcacagg aatttggttc tcattatgaa ggggatatcg
    39841 aattcgcaac agatttgaag acttttttca atattactca tatggacacc atttatattg
    39901 cttctccgaa tagtcttcac tttagtcaag ctaagcaagc aattttggca ggaaaaaatg
    39961 tgatagttga aaagccagct ttttcgacgc caaaagaaat ggatgaaatc atccatttgg
    40021 caaatgaaca caatgtgtat ttttttgaag cagcaagaaa tatccatgaa tcaggtttta
    40081 aaaagattgc cgattactta ccattaaaag acgagataat cggtgcaaat ttcagctata
    40141 tgaaatattc ttcgcgctat gaccaagtat tagatggaga agaaccgaat atcttctcga
    40201 ctcatttctc aggtggtgca ttgatggatt taggtgttta ccttgtatat gcagctgttg
    40261 cttggtttgg aatgccaaaa gaagtccatt atttcccaag aaagatttct actggtgtag
    40321 atggtcttgg atgggggatc ttacgttatg agaattttga tgtatcgatc cagccaggta
    40381 aaatcggtga ttcttattta ccgtcagaga tttattttga ctcaggaacg ctagtgatga
    40441 atggagtcaa tgcaattgaa caagcacaat ttcatgatcg cgcgcataaa gaaatcattg
    40501 atttggcggt aacagctgca gaaaatccaa tggcagatga agcagaggat tttgccaatg
    40561 tcatggctca tccgaaagat ccagcttggg gattgttgta tgaagagtgg atcgagctag
    40621 ctagaaatgt gaaccaagtg atttatgatt taagaaaaaa tggcggcatc aaattcgatg
    40681 cagataatga ggaagatttt taatgaacga atttgtacaa gtatttcccg aaaattggca
    40741 gaaaaaatat gaggataaag ggtttatctc accttctctt attcaacgag cagtttttca
    40801 gccactaagt aataaacaaa gtatagtagc ggtttctcct acaggttcag gtaaaacact
    40861 ggcttatctt tggcctctac tactaaacgt ggaaccaggt gaagcaagtg cgttggtcat
    40921 ttttgcttct tctcaagaac tagctattca agtagcagat gttgcacgtg aatggggaaa
    40981 agacaaagag ttgaaagtcc aatctttggt gggcggagcc aacgtcaaac gtcagattga
    41041 gggtttgaag gaaaaaccag aaatcttgat tggtacacca ggaagaatat tggaattgat
    41101 gaaagcaaaa aaaataaaag cacatcaagt gaaaacaatg gtatttgatg aagcagacca
    41161 gctatttgac gctgggaaca gccaaatcat tgatcaaatt cttcatcaag ctccaacaga
    41221 gtatcagcta gctttttttt cagcgacagc agatcgttcc cttgaaagca ttgaaaaaat
    41281 cactgggaaa acgtttgaga caattgacgt gacagcagag gacgattctc gaaaaggact
    41341 gagacattac tttttacgtg taccgatacg aaagaaagat gactacttga gacgtctcac
    41401 gcatatagaa gattttcatg gtctgatttt ctttaatcaa ctgactgaat taggcgtaat
    41461 ggaagaaaaa ttgctgtata gaggtgtacc ggtaggaagt ctggcctctg atcagaacaa
    41521 gctactccga aaagcagcgc tagaacaatt caaaaagaaa atggtcagag cacttttgac
    41581 cacagatgtg gcggctagag gtttagatat tacaggtctt ccttatgtag tgaatgcaga
    41641 agttccgtta agtgaagaag cttatttgca tcgttcggga agaactggtc gaatgggtaa
    41701 tgaaggaata gtaattacac tcgtacaaga taataatcta agagatttaa aacggctttt
    41761 acgtccatca aatataacac ttgaagagat ttttttatac ggaggtaagc tgcaaactga
    41821 gcagccaacc aaagaagatg cagctcctgc agtaaataaa aagtatgcga aaactttttc
    41881 tggaaaggag cagaacccag aatataacaa agaaaaaata agaaaaaaag ttaaaaataa
    41941 atcaaaaaaa gagagaaata aaggagcaag gcgcaaataa gcgccttttt atgctatgat
    42001 tatgaaaacg ggaacaaaat tggttgccag aggaggggtt atgtgaaaga acgtcaagaa
    42061 gagatcttgc agctattgac agaaaaccaa caaggattga cagcaagtga tgtagctgag
    42121 cgcttaacta tcgatcgaag caatgcaagc cgatatttaa gtgaactata caaggcccat
    42181 catattgtaa aaaccgctgg ccgaccagtc gtttactctt tgccaacgga aaagtcaaaa
    42241 tctgatgaag tgcatgtcga ctcttctacg caagtgactt ttgaaacact cgttggagaa
    42301 aatgattctt taaaagtcag catacagcag gcaaaagctg cgattcttta tccaccgcgg
    42361 gggttgcata cgattatttt tggagaaact ggtacaggga agtcgatgtt tgctgaatgt
    42421 atgtatcatt ttgcaatcga ttcagagatg ctttctgcag atgcgccatt tgtttctttc
    42481 aattgtgcgg attatgctca aaatccgcaa ctgctctttg gacacatctt tgggatcaaa
    42541 aaaggggctt acacaggagc agctcaagat agtcccggtc taatagccaa agcagacggc
    42601 ggtatattgt ttttagatga gatccaccgt ttaccgccgg aagggcagga aatgctgttt
    42661 actttcattg ataaaggtat ttaccgtccg cttggagaaa gtgcacaagt acatgaagca
    42721 tctgttcaga tcattggggc gacgactgaa tcttcagaaa gtttcttatc tacgttcaat
    42781 cgacggattc ctatggcaat caccttgccg aatcttgctt cacgttcact ggatgagcgt
    42841 tatgaaatca tctctctttt tatcaaacaa gaagcgaatc gtttgaatca acgtatcgat
    42901 atcgaaaaag aagcgattct tgccttcatg ctttatgatg cggaaggaaa tatcggtcaa
    42961 gtgaaacgag acttgaaact agtctgtgca aaatcttttt tacattaccg tactcatcat
    43021 gagaaaaagc taatcattcg aaaagaagaa ctttcccttc aagtacaaaa aggattactg
    43081 aaaatcaagg aaataccgga acgtctcgac cgattcatgg acagcaaaag tcagtatttg
    43141 acatttgaac caggatttgc tgatgttgtc tggtcacaag atccagaacg aaacatgcag
    43201 gtctataacg atattgagga aaaagtcact tctctttccg aaaccggtgt ggagaacatt
    43261 gatctagaga ccctcatttc aaaagatgtg gatgcttatt tccagacata tgtcaaagaa
    43321 ttgactaaaa gttcgattcc caaagatctt ttgccagatg aaatctggcg gttaaccaat
    43381 caattgtatg atgtggctga aaaacggtta gaccgtattt ataatgaaaa agctcgtttt
    43441 gcttttgctt tgcatctgca aagtacattg gatcgtgtga gagaaggtca tatgatcgtc
    43501 cacccagatt tgaataacgt acggaaaaat tttaagaggg aattccaggt agcaatcgat
    43561 ctttcaacga tcattgaaga agaagaacaa gttgagattc cttttgatga aatcggcttt
    43621 attagtatgt ttctttctat caatctagga gaaagtgagc ctgttaaaga aaacaaagta
    43681 gatgttctca tattgatgca cggcagacag actgcttcaa gcatgttgga gatggcacaa
    43741 gaactattag gaacaaaagt agggaaagct tttaatatgc cactggttat ggaagttcaa
    43801 acaatgtatg aagaagtgtt acaatatgtc aaaaactatc aagagcattt gacgaatggc
    43861 ctactcttgt taacagatat gggatcatta aacaccttca gtgatttgat ttatgaagag
    43921 actggcattc ggacgaaagc tatcacgatg acaagtacca tgattgtgat agaagcgata
    43981 cggatggcaa gtgttggaag aagcttagaa gatatttatc aaaatatcca gctttccttt
    44041 gaatcgatcg tacgtgatca gttcaaagcg gaaaagtctg aaactttaca gcgtaaaaaa
    44101 gccgtaatcg tgacatgctt tactggtgaa ggagtagctg ccaaattata tcagcgtatt
    44161 atgccagtga ttaatcaaaa taaggtagaa ctgatccaga tgcagttttt agaacgtgaa
    44221 tcattcaaaa aacacatcga tgccttattg gaagaatatg agatcaaagc aattgcggga
    44281 acagttgatg ttgattatca aaatatccca tttttttcag cctatgacgt ttttgatgat
    44341 gaaaaactaa atattttaaa gcgtatagct gatgatgaga tcccgacagc taaaattgtc
    44401 gattcacttg ctggatcatt aagcgatgtc ttttctattg atgaactgat ccgttcattg
    44461 caaaagacag ttcagcagat ccagaccgat ttgtcgatta tcgtggaacc tggagtagat
    44521 actggaatcg ttatccattt agccttcatg ttagacggat tgatcaaaca agaaagccat
    44581 cggcaatttg agaaactgtc tattttctca aaaatttatc gtttggaaat ggatatcgtt
    44641 cgggcaaact tgatggcact agaacgaaaa tatcaggtaa aaattccaga agatgatatc
    44701 gcatttctta cacaaatgtt tcttgaaaat aaaataaaaa aatgaacaca ctaatcatac
    44761 tgtttacaca ctaaacatct gtgtgcgcac tataaaaaag agctaaaaag catgaaaacg
    44821 ctatttcagc tctttttttt attggcatgc ttcttgcgtt atataaaagt gtaaaaaaat
    44881 aaaaggagtg ttaaaaatgg caaaaaaaac aatcatgtta gtttgttcag cagggatgag
    44941 tacgagttta ttggtcacaa aaatgcaaaa agcagcagaa gaaaaaggga tggaagccga
    45001 tatttttgcg gtctctgcct cagatgcaga taataattta gaatctaaga atgtcgatgt
    45061 attattatta ggtcctcaag tacgtttcat gaaagctcaa tttgaacaaa aacttgcgcc
    45121 taaaggtatt ccattagatg tcatcaatat gcaagactat ggcatgatga acggagaaaa
    45181 agttcttgca caagcagaaa atctaatgaa ataaatcaag tatgcagttg ggggtataac
    45241 aacgtggaag atcaacaaaa tctagaagcc atcatggggt taatcatgtt tggcggtaat
    45301 gcaaagagcg atgcaatgga agcgattgcg gctgctaaaa tcggtgattt tgagttagcg
    45361 gataggaaaa tcgctgatgc agaagaatca cttatacaag cgcatcatgc acaaacagac
    45421 atgttgacac aagaagcaca aggaaatcat atgcaagtga cattattgac tgttcacagt
    45481 caagatcact taatgacatc tattgcgttt actgatttag caaaagaaat catcgaactt
    45541 tatcgtcgcg tatacagcga ataaatacaa cttctttttt gaacggaaga agaaaaattt
    45601 ttgaacggat tttttatttt gttataagga gaactctctt tatataaaaa acttatagga
    45661 ggaagtaaaa aatgaacgga ctatcaagtt ggatggagaa atacatcctg ccggttgccg
    45721 caaaaatcgg agcgcaaaaa catttagttg ctttgcgtga tgcttttatc ggcatactgc
    45781 ccgcaacaat ggcgggatca gtcgctgtaa tgatcaatgc aattattcgt gatttaccgc
    45841 aacagtttaa cagtacctat gctgacagtt tagcagcaga caaaggaatc tttgccgtat
    45901 tgaatgtcat catcggaatc aatggttttg tctggaacgg tacactagca atcgccggat
    45961 tgatctttgc cttttcttgg gggtataatc ttgcccgagc ttatggagta aatgatctag
    46021 ctggggggat cgttggttta gctaccttga ttcaaggtat ttcctttggt ccagcattat
    46081 cagcagcatt agatgtgaat gttccttcaa atgtcgcaga tgcaatcaat agttctgaga
    46141 ttggtgcaac gattgcagat ggcacactgt cagttggatt atggggttgg ctaaaactag
    46201 atcatttgaa tggaaacgct tattttacag ttatgatcat gggagctatt tcagtcatta
    46261 ttttttgtaa attgatgctt gccaacatta cgatcaaaat gcctgattca gtaccaccag
    46321 cagtatctaa agcatttgca gccattttac cagcgacgat cgctttatac gtcattgcta
    46381 ttatcaatta tatcgtaggt cgtgtagctg atgggcaact gatcattgat ttggtgcaaa
    46441 aatatattgc agaaccattc ctaggattat ctcaagggtt gggagcagta ttgatcgtca
    46501 cagtatttgt tcaagtattc tggttctttg gtattcatgg atcaaatgta ttggcaccag
    46561 tgctagaggg aatctgggga caagcacaac taatcaatat tgatattttc caaaaaggtt
    46621 ataacggaaa aactggtaca ccagccgtgt tagatgcaat cgaagatgga aaagcttata
    46681 tgtgggtccg cggatcgttc gatgcttttg catggtttgg cggatcaggc ggaacgatcg
    46741 tcttactgat agctatcttg attttctcta aacgtgcaga ttatctaaca gtagctaaat
    46801 tatccattgg gcctggtatc ttcaatatca acgaaccagt gatgtttggt ctgccaatcg
    46861 tattgaatgc aatcatgttt atcccattct tgattgctcc agtagtagca acagcaattg
    46921 gatggacagc tacttatctt ggcctagtcg ctccggtatc gcagcaagtc acatgggtag
    46981 taccgccgtt cttattgtca ttcttagcaa caggagcgga ttggcgggca ccgatcgtaa
    47041 cattagtatg tatggtcgtg actttcttga tttgggcacc atttgtcatc tctgcaaaca
    47101 aaatggaccc tgccgcaggc gaacaacaat aagatgaacg tgaggagcgg gacgctttaa
    47161 gtttcgttcc tcataatgaa ttttcgagga ggaactgaaa tgattacaat tgccagtgca
    47221 atgcaacaag atgaaatcaa agaattgtta gaaggataca atgaagaagg aatgactttt
    47281 acatttaaag aaaaacaagg aatcaagctg ttctttgaaa caagtgcaga agatccggaa
    47341 gttgcggcta aaaaagcaaa ggaactaatc aaagcacagc cttggggaac agtcctttat
    47401 tttcaatcaa cagctgttta gaaaatggtt taaaaagata ggatggttga gatgataaaa
    47461 aacggtttat ttttatgtag tattggttct ttgatcgtgt tgtatagttt gaatccacat
    47521 tcgcaaaggt atgatttgta tagtatggtc acaggtcttt ttctaacagg attaggaatc
    47581 tatttcttca tgaaggggaa aaaaagagaa gaagcaaagg agaaaaataa ctaatggaaa
    47641 tcacgaaaaa attaaatatt ccagcatcgt ttttttacga taaagttatg gactctgtcc
    47701 ttttcgatgt acgtaaagcg accggcaaat cagtaactag aaaacagctg aaaaatttgg
    47761 agtacgtcaa acaattctcg caaaataacc gggctcgcat aaaaatcgaa gaagtcattg
    47821 aaaaccagtc ttataagttt cgtacatcga caacgaagaa tgaattcgtc gttcattatc
    47881 aaatcaaacc attggacgat tcgacctgtg aggttcatta cacagaaaaa atggagtctt
    47941 atggcatgct tcaaaagttg aatgatatgc ttttaggaac aatcttgatg tattttaaac
    48001 ggagacgttt caaaaaaatg ctagatatga tcgaagcaac gtattaaatc aatgaaacat
    48061 ggtactattt agttattgaa aaatgaagta agctagaagg aatttttcat aggaagatag
    48121 atctttcctg cgaatcctca ctagcttttt tgtaggaagg ggcagcaaaa tgacaaacaa
    48181 agtaagagag ctaggtgtat cttcgattga ggaaatgttt gatttagcca cttatgcatt
    48241 taatggggca gatacacctg aaaggcgaga acgttttcgt acacttgcgg agaattcttg
    48301 gaattacgga ttttttgatg aggaggatcg gctaacaagt caagtaatgg cgaccccttt
    48361 tccagtgaat ttttacggac aagagtacct aatggccggc attggctatg ttgcttctta
    48421 tccagaagca aggggacaag gcgggatcaa tcagatcatg gaaaagatcc ttgaagattg
    48481 ccgtgatcgt aaagtaagct tatcttatct ggcgccattc tcctatccct tctatcggcg
    48541 ctatggctat gaacaatcat ttgataaaat cagttatcaa ctaaacagtc gtgatgtgcc
    48601 gtttatcaaa aaaaaatctg gaaaaataaa gcgtatggat tttgaagatg caaaagctgc
    48661 aatcagaaga atttatgaac agatgccgga aaaccaacga gctggtctga agcgcgaaga
    48721 ctggtggtat acgtataaat tcaagcaaaa aggcaattac cagtttgctt tgcattttga
    48781 tgataatgag gaagcaacgg gatatatcgt ttatcagctt gcaacgtctt cctttattat
    48841 tgcggagtgg ggatttttgg atcatgatgc tttttgcagc cttgttcgct tcgtaagttc
    48901 tcacaatggt gcttttgaaa catttgaata ttcatgcggc tacggcggta atgatcaaaa
    48961 ttatctatta gaaaatccat ttgcttctgt aatgatcaca ccttatatga tggcgaggat
    49021 cgtagatatt tctttgtttt tggaaaatta tccatttaaa aagagacaag ctgcatttgc
    49081 cctacaaata gaagaagatc attatgcgaa atggaatgaa ggaatcgtgg aagtaacaat
    49141 cgaaaatgga aaaaatcatc tctcaaaagt agaacaaaca aaactcccaa tcgttcgagg
    49201 aagtatccaa catatcacgc agctattatt agggtatcgt aaagtggaag agttggtatt
    49261 ccaagaaaga atacaggtga caaaagaata tgaagaaacg ttaagctcaa tcttgccaca
    49321 gcaacagcca attcttaatg actatttttg ataaaaaacg ggtgatgaca atatttttgt
    49381 caccaccctt ttgtttttca tttggcattt gaaggaaaat gttaaagtaa atcctctttt
    49441 ttcgtaaatt tgaatttttt gttttctttt gttttagggt tttctttttt gtggtcttgg
    49501 attgcttctt tcgtaatctt ttcccgattt tttttagatg aatctaagcg ttctgcgtat
    49561 actttttctt ttggcttatc attcattgga aaggacctcc ctacattatt tcattctacc
    49621 tctagtttat catctgttaa ggactagtgg aaacgttttg cctaacttat aagctgattt
    49681 ttttagcaga taagcttgca agtaaaaaac aaacaagcta caattgattt attgaaattg
    49741 ttcctgtagc tcagtaggat agagcaatcg cctcctaagc gataggtcgc tggttcaatt
    49801 ccagtcagga acgtataaat aacccctgta tcctttgcgg tacaggggtt tgttttgtat
    49861 ttgtgccaaa aatgtgccaa actagttatc atttttatat aaactatcga acacagtatt
    49921 gattttttct ttatcctgat cttcaagctc tcgaacaata tgaagataag tagacattgt
    49981 agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta agtatattta ctccttgata
    50041 aagtaaaata gatgcatgtg tatgccttag tccatgcaaa gtaaattgct tttcaatccc
    50101 taattctttt aattttttgc gcaaatattt gctgacggcg tttgatgaaa ctaatccatt
    50161 ttttaagttg aaaaaaacaa aattatgagg gtttttgatt tcaaaattct cgaataattc
    50221 tttttgattg attttaaact tttttaagag atcaatcgta tgtttatcaa tggaaatttt
    50281 cctatttgaa gtttcgtttt ttgtttttcc ccattcattt aatttataat tccatgtttt
    50341 attaatagtg atgatttgat cttcgaaatc gatatcttcc catgttaatc ctagaagttc
    50401 agcaaatcgc attccagtgg caccagcaac taacacgagc ataggagaag agtactgcgc
    50461 agaaaggttt tcttctacga gtttcattaa agatttaaat tgatcataat ctaaatattt
    50521 atcttctttt ggcttcaatg aatcttttcc tttgattact gcttttctag ttggatcaaa
    50581 cggaattaat ccttcttcta cggcatcttt caacgacgct ctaatatggt tattaaactt
    50641 aataacggta gattttacat ggtctttagc atacttattt aaaaagcgtt gatatcccac
    50701 tctatctaaa tcagaaatca ataccgctgg catatatttc tttatgttca ttaacgtatc
    50761 ttcgtatttt ctataagtga taggagatac tgttccttct ttataaagtt gcatccaatc
    50821 ctcaaagtaa tctgataata atagattttt gcgatcccct ttaaggcctt tgttcaagtt
    50881 atattctaat tcgttggcgg catcttttgc ttctcctttt gtcttaaatc ctgattttct
    50941 tatcttcgtg tattttccgt catctttttt gtatgaaatt tcgtattgcc aactatttcc
    51001 acgtttgact aatcttgcca taattgaatc agctctcttt ctctgataca ataggaacta
    51061 taaagaagcc cattgtttgg gttttattat tttttgcaca tcctttttgc ttgccggcgc
    51121 aaggatgtgc tatttgtcgt tttaatggta taattggatg tgtattagat catccaatca
    51181 gaggtgttta ttatgattaa agtgattttt aaaagaataa ctgaacttcc agagtttaga
    51241 ttatttgttg attattggta tgtctggttt tttattgttg cattgctgat aggtctagat
    51301 gtcatattag aaagaaaact taagtaagag ttgagttaat aaattgaatt cctaaatgag
    51361 atattttgta gcccatattt tgtttgccga catcacctaa tactatggag ctaatcgttt
    51421 tttctaacag accaaaatca gataatctat ttaagctacc atctattagc tcttgtgaat
    51481 agccagacat gctgatattt ccagtgaaat tttcgtttcc acgactcatt aagcatgcta
    51541 acaattgaat ctcaatcgat gttaattgat tcagtacaag aagataatac tcctcgttat
    51601 cccatgaagc gttgttaatt cgaagtaaac aattcttata aagattttta tacatatcta
    51661 ttttattttg tgcctttgat ttttctacct cgtcatttat cccttcgaat attccaatga
    51721 gttggtcctt gttctctaag ttgaaaacat cttctggaat ttgatcgcga atgttttcta
    51781 atgattcgtt taaatttttg tagaatttct cgatgcgttt gaaacgcttc tcgtttttag
    51841 acccaaaata aagtgactgc acaacgccac caatataagg aattgctcca ataccgcctt
    51901 caactattaa ttcaagatat tcttttttgg ttagtttttc attgctatta tccatagtta
    51961 cctcctattt tgttatttcc tttacaagca ttgtgactcc tgatggcact cttttgtata
    52021 ggttttatta ttgtgcacaa tctaactctt agtggggcgt ggttcattta ttttttactt
    52081 actttactat taatcatatc ccattgagca taggattttt ctatgttcga tcctcttgaa
    52141 ctaatttctc ctgtaaaagt tactttatcg ccctttgtaa aggtatcagc attgtttaag
    52201 tccttcgcaa aaattacata tgaaacttta ggtgttgcag aaatatcatt ccaactttta
    52261 tcatcgtact ctttgtctgc tataatggct attctagtag tcattgattc gataacagtt
    52321 ccttcaaaag tatatgtttt accactaata tatttagtaa aagctgtatt ttgctcagta
    52381 gctggagtaa tagaataata actatctaag aaagcacccc agtcatgatt atttgtctct
    52441 aaaaattctt caaattgagt taggctttta ttgctgctgt tagatgtagt agtttgagta
    52501 tcagatgatg agctttcctg tttgttattg tcatttgcct cgtttgatga ggaaagcgag
    52561 aaacctacgg tagtaaaaat aatcccgata ataaggagaa tcataggtgt tttcttattt
    52621 gtttttttga taaatcttac aattaacatg attaaaccta taataaataa aacaatacca
    52681 gtaaaaccaa atataccgtc cattaataga tcctctttct cttttgatat tgaatattat
    52741 aaaccaagca attctttttt cttaaaatcg aattcttctt gattaataat cccttcatct
    52801 aacaactctt tatacttttt aatttcatct gcacttgaaa tttgtgtttc tttggaatta
    52861 ttatctaata tagattgttg cttctctata tattttttta aattttccat ttcattccaa
    52921 tatttttttg taaacataac ggtgttttca tctttcgtgg ccgctaatat acctccgcga
    52981 ctttcgtttc cgcctagcaa ggtaaattgt atataaccat tactcatacc tggttttttt
    53041 aattgtacgg ctgatatatt tttaaagggg atagtttttt ctcctttaat accttgatta
    53101 actaaattta taaatccttt tctaataatt gaaatagaat catcagttac tttaatcaag
    53161 gtttttcctg gagattttat taaaatctct ttttccattt tttcttcctc atttctgtga
    53221 tatg
//
LOCUS       DAFEHS010000010        51141 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFEHS010000010
VERSION     DAFEHS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.010"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            212..604
                     /locus_tag="LP327_000649"
     CDS             212..604
                     /locus_tag="LP327_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL1724793.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="LP327_000650"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="LP327_000650"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBL1724794.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="LP327_000651"
     CDS             3165..4775
                     /locus_tag="LP327_000651"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBL1724795.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="LP327_000652"
     CDS             5067..5936
                     /locus_tag="LP327_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBL1724796.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="LP327_000653"
     CDS             6101..7372
                     /locus_tag="LP327_000653"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL1724797.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="LP327_000654"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="LP327_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBL1724798.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="LP327_000655"
     CDS             8763..9026
                     /locus_tag="LP327_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBL1724799.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="LP327_000656"
     CDS             9471..9920
                     /locus_tag="LP327_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL1724800.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="LP327_000657"
     CDS             9977..11395
                     /locus_tag="LP327_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL1724801.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="LP327_000658"
     CDS             11395..12255
                     /locus_tag="LP327_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBL1724802.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="LP327_000659"
     CDS             12429..13202
                     /locus_tag="LP327_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL1724803.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="LP327_000660"
     CDS             13349..13714
                     /locus_tag="LP327_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBL1724804.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="LP327_000661"
     CDS             complement(13862..15070)
                     /locus_tag="LP327_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL1724805.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="LP327_000662"
     CDS             complement(15089..15367)
                     /locus_tag="LP327_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724806.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="LP327_000663"
     CDS             15521..16216
                     /locus_tag="LP327_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL1724807.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="LP327_000664"
     CDS             complement(16658..17455)
                     /locus_tag="LP327_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBL1724808.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="LP327_000665"
     CDS             complement(17452..18636)
                     /locus_tag="LP327_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL1724809.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="LP327_000666"
     CDS             complement(18661..19908)
                     /locus_tag="LP327_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL1724810.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="LP327_000667"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="LP327_000667"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBL1724811.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="LP327_000668"
     CDS             21888..22202
                     /locus_tag="LP327_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBL1724812.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="LP327_000669"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="LP327_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBL1724813.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="LP327_000670"
     CDS             22887..23531
                     /locus_tag="LP327_000670"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBL1724814.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="LP327_000671"
     CDS             23546..23875
                     /locus_tag="LP327_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBL1724815.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="LP327_000672"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="LP327_000672"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBL1724816.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="LP327_000673"
     CDS             24863..25687
                     /locus_tag="LP327_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBL1724817.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="LP327_000674"
     CDS             25680..26027
                     /locus_tag="LP327_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBL1724818.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="LP327_000675"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="LP327_000675"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBL1724819.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="LP327_000676"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="LP327_000676"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBL1724820.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LP327_000677"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LP327_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBL1724821.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LP327_000678"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LP327_000678"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBL1724822.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="LP327_000679"
     CDS             31657..32451
                     /locus_tag="LP327_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1724823.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="LP327_000680"
     CDS             32441..34483
                     /locus_tag="LP327_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1724824.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="LP327_000681"
     CDS             34764..34913
                     /locus_tag="LP327_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBL1724825.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="LP327_000682"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="LP327_000682"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBL1724826.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="LP327_000683"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="LP327_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBL1724827.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="LP327_000684"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="LP327_000684"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBL1724828.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="LP327_000685"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="LP327_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBL1724829.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="LP327_000686"
     CDS             39330..40412
                     /locus_tag="LP327_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBL1724830.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="LP327_000687"
     CDS             40412..41215
                     /locus_tag="LP327_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL1724831.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="LP327_000688"
     CDS             41285..42025
                     /locus_tag="LP327_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL1724832.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="LP327_000689"
     CDS             42036..43040
                     /locus_tag="LP327_000689"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBL1724833.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="LP327_000690"
     CDS             complement(43085..44929)
                     /locus_tag="LP327_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724834.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="LP327_000691"
     CDS             45141..45659
                     /locus_tag="LP327_000691"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL1724835.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="LP327_000692"
     CDS             45787..46470
                     /locus_tag="LP327_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBL1724836.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="LP327_000693"
     tRNA            46779..46852
                     /locus_tag="LP327_000693"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="LP327_000694"
     CDS             47011..47370
                     /locus_tag="LP327_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBL1724837.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="LP327_000695"
     CDS             47493..49124
                     /locus_tag="LP327_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBL1724838.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="LP327_000696"
     CDS             complement(49186..49827)
                     /locus_tag="LP327_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL1724839.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50013..50089
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="LP327_000697"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="LP327_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBL1724840.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="LP327_000698"
     CDS             50444..50788
                     /locus_tag="LP327_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBL1724841.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="LP327_000699"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="LP327_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBL1724842.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT        16406 a         8862 c        10661 g        15212 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatttacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaac tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc c
//
LOCUS       DAFEHS010000011        50045 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFEHS010000011
VERSION     DAFEHS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.011"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="LP327_000700"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="LP327_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL1724843.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="LP327_000701"
     CDS             complement(322..891)
                     /locus_tag="LP327_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBL1724844.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="LP327_000702"
     CDS             1077..1979
                     /locus_tag="LP327_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL1724845.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIVLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="LP327_000703"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="LP327_000703"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBL1724846.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="LP327_000704"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="LP327_000704"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBL1724847.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="LP327_000705"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="LP327_000705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBL1724848.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="LP327_000706"
     CDS             8547..8732
                     /locus_tag="LP327_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBL1724849.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="LP327_000707"
     CDS             complement(8794..9990)
                     /locus_tag="LP327_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL1724850.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="LP327_000708"
     CDS             complement(10160..11596)
                     /locus_tag="LP327_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL1724851.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="LP327_000709"
     CDS             11889..12698
                     /locus_tag="LP327_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL1724852.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="LP327_000710"
     CDS             complement(12884..15037)
                     /locus_tag="LP327_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBL1724853.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15432..16280)
                     /locus_tag="LP327_000711"
     CDS             complement(15432..16280)
                     /locus_tag="LP327_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL1724854.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16504..18444)
                     /gene="thrS"
                     /locus_tag="LP327_000712"
     CDS             complement(16504..18444)
                     /gene="thrS"
                     /locus_tag="LP327_000712"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBL1724855.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18781..18960)
                     /locus_tag="LP327_000713"
     CDS             complement(18781..18960)
                     /locus_tag="LP327_000713"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBL1724856.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(19007..20830)
                     /gene="cadA"
                     /locus_tag="LP327_000714"
     CDS             complement(19007..20830)
                     /gene="cadA"
                     /locus_tag="LP327_000714"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL1724857.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21108..23387
                     /locus_tag="LP327_000715"
     CDS             21108..23387
                     /locus_tag="LP327_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL1724858.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23480..24127)
                     /locus_tag="LP327_000716"
     CDS             complement(23480..24127)
                     /locus_tag="LP327_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724859.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24306..25025)
                     /locus_tag="LP327_000717"
     CDS             complement(24306..25025)
                     /locus_tag="LP327_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1724860.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25156..26307)
                     /locus_tag="LP327_000718"
     CDS             complement(25156..26307)
                     /locus_tag="LP327_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL1724861.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26524..27264)
                     /locus_tag="LP327_000719"
     CDS             complement(26524..27264)
                     /locus_tag="LP327_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL1724862.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27268..27591)
                     /gene="rsfS"
                     /locus_tag="LP327_000720"
     CDS             complement(27268..27591)
                     /gene="rsfS"
                     /locus_tag="LP327_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBL1724863.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27648..28241)
                     /gene="yqeK"
                     /locus_tag="LP327_000721"
     CDS             complement(27648..28241)
                     /gene="yqeK"
                     /locus_tag="LP327_000721"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBL1724864.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28225..28875)
                     /locus_tag="LP327_000722"
     CDS             complement(28225..28875)
                     /locus_tag="LP327_000722"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBL1724865.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28885..29196)
                     /gene="yhbY"
                     /locus_tag="LP327_000723"
     CDS             complement(28885..29196)
                     /gene="yhbY"
                     /locus_tag="LP327_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBL1724866.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29212..30321)
                     /gene="yqeH"
                     /locus_tag="LP327_000724"
     CDS             complement(29212..30321)
                     /gene="yqeH"
                     /locus_tag="LP327_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBL1724867.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKENKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30318..30845)
                     /locus_tag="LP327_000725"
     CDS             complement(30318..30845)
                     /locus_tag="LP327_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBL1724868.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(31025..31810)
                     /locus_tag="LP327_000726"
     CDS             complement(31025..31810)
                     /locus_tag="LP327_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBL1724869.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31803..32672)
                     /locus_tag="LP327_000727"
     CDS             complement(31803..32672)
                     /locus_tag="LP327_000727"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBL1724870.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32673..34052)
                     /locus_tag="LP327_000728"
     CDS             complement(32673..34052)
                     /locus_tag="LP327_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBL1724871.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(34060..34479)
                     /gene="fabZ"
                     /locus_tag="LP327_000729"
     CDS             complement(34060..34479)
                     /gene="fabZ"
                     /locus_tag="LP327_000729"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL1724872.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34504..34980)
                     /gene="accB"
                     /locus_tag="LP327_000730"
     CDS             complement(34504..34980)
                     /gene="accB"
                     /locus_tag="LP327_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBL1724873.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34983..36218)
                     /gene="fabF"
                     /locus_tag="LP327_000731"
     CDS             complement(34983..36218)
                     /gene="fabF"
                     /locus_tag="LP327_000731"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBL1724874.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36237..36974)
                     /gene="fabG"
                     /locus_tag="LP327_000732"
     CDS             complement(36237..36974)
                     /gene="fabG"
                     /locus_tag="LP327_000732"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBL1724875.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36986..37903)
                     /gene="fabD"
                     /locus_tag="LP327_000733"
     CDS             complement(36986..37903)
                     /gene="fabD"
                     /locus_tag="LP327_000733"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL1724876.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(38010..38234)
                     /locus_tag="LP327_000734"
     CDS             complement(38010..38234)
                     /locus_tag="LP327_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL1724877.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38287..39249)
                     /locus_tag="LP327_000735"
     CDS             complement(38287..39249)
                     /locus_tag="LP327_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBL1724878.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(39259..39699)
                     /locus_tag="LP327_000736"
     CDS             complement(39259..39699)
                     /locus_tag="LP327_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL1724879.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            40005..40970
                     /locus_tag="LP327_000737"
     CDS             40005..40970
                     /locus_tag="LP327_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL1724880.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(41027..41257)
                     /locus_tag="LP327_000738"
     CDS             complement(41027..41257)
                     /locus_tag="LP327_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBL1724881.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(41343..42230)
                     /locus_tag="LP327_000739"
     CDS             complement(41343..42230)
                     /locus_tag="LP327_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBL1724882.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(42319..43542)
                     /locus_tag="LP327_000740"
     CDS             complement(42319..43542)
                     /locus_tag="LP327_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL1724883.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(43559..44605)
                     /locus_tag="LP327_000741"
     CDS             complement(43559..44605)
                     /locus_tag="LP327_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL1724884.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(44769..46496)
                     /gene="ptsP"
                     /locus_tag="LP327_000742"
     CDS             complement(44769..46496)
                     /gene="ptsP"
                     /locus_tag="LP327_000742"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBL1724885.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(46496..46762)
                     /locus_tag="LP327_000743"
     CDS             complement(46496..46762)
                     /locus_tag="LP327_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBL1724886.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            47241..49475
                     /locus_tag="LP327_000744"
     CDS             47241..49475
                     /locus_tag="LP327_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL1724887.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            49623..49943
                     /locus_tag="LP327_000745"
     CDS             49623..49943
                     /locus_tag="LP327_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL1724888.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        14304 a        10738 c         8749 g        16254 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agctgctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgttt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaacaa gaagtattta atattttttt
    15121 cataaaatct ttcatcattt cctatccttc cgcctattta cattcatgct tcattgtacc
    15181 caaaaacttc ttaataaaaa agaaaaaatg ggcggagcaa gaaattcttt acctaatttc
    15241 aagataaaat caactttttg tttcacagca atcacttaaa gttacatatc caactatttt
    15301 tttcattaaa aaataaactt atttttgaaa attatgtttt cttaagtcgt gaaacacttt
    15361 gatggatcat ttatttgtgg aaaactacgt tttgttattc taaaaaactt atataaaaaa
    15421 tagtaaggtt tttatttcga atccataaaa tattcttgga ttgcttctcg tgtttttttc
    15481 actttttcgc taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta
    15541 gcaaattggg ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta
    15601 gatgtttgta cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac
    15661 gcaatgattt cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt
    15721 ctacctgagt ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg
    15781 agcaagacgt ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc
    15841 agaatatctt ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc
    15901 gattccaaac actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct
    15961 tttatcgcta cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt
    16021 gcttctatat cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt
    16081 ttgaagtccg tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca
    16141 ctcaacccgg aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta
    16201 ccgataatgt agttggccaa ctttttttct gctgaagaat aatcttccat catactttca
    16261 atacgactaa ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata
    16321 ccatatcaga cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc
    16381 aacagaatat cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga
    16441 atacctgtta tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac
    16501 ctattagttt tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt
    16561 caaatcttct acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc
    16621 caattccttg tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat
    16681 cttatagccc attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa
    16741 acgttctttg atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt
    16801 tgcttgtata ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc
    16861 gacaaaacgt tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt
    16921 ttccccgtct tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg
    16981 tatagttgat aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg
    17041 tccgtaaaag gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc
    17101 ggctttcagc attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt
    17161 attgtttgga tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata
    17221 tactgccacc atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac
    17281 aaatgtatga ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc
    17341 tgatttttca tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata
    17401 agaatggatc gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac
    17461 aagcatttcg ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca
    17521 gtgtccagat gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta
    17581 acctagacta acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc
    17641 ttttggaagc cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag
    17701 ttcttttcct agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc
    17761 tttcaaagct ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt
    17821 tgagtttcca cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc
    17881 agtggatgga acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt
    17941 aatcacttct tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa
    18001 aatttctaat gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac
    18061 gattttcatc atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc
    18121 attatctgta tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt
    18181 tggatagagg cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc
    18241 aagtgcatct tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg
    18301 agtcaaatca acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt
    18361 tgaaatgctt tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc
    18421 atctggaaaa gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga
    18481 tcatcaagct gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa
    18541 agttcgtggt tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc
    18601 gaccgccctt gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa
    18661 agtttcagcc tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat
    18721 caatcaatta tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac
    18781 ttattttttc cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac
    18841 atggatgtgt tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat
    18901 catattttct aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat
    18961 aatgctctct cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg
    19021 tcttgggtac tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa
    19081 tagagaccgt atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg
    19141 tacttgcaat taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg
    19201 ctattctcat cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt
    19261 tcaagatgac cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa
    19321 taccgatatc tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa
    19381 caggtctttg atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac
    19441 gggcatgtac agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat
    19501 ccccggtgat catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg
    19561 ctgcttctgg gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg
    19621 ttacatagat tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc
    19681 caatctttac ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca
    19741 gttgactaac aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga
    19801 tatggctaga ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc
    19861 cattgactgg ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat
    19921 caaaagcaat cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga
    19981 tcccgttttt acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca
    20041 gtggacaagg tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc
    20101 accaagaaat accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag
    20161 ctaaacggac aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt
    20221 gatactggct gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta
    20281 aagaccctga cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca
    20341 aagaagattc atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt
    20401 cgccaggctt gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca
    20461 attttccttc tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt
    20521 ctttacccgc tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca
    20581 atatcatcaa actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg
    20641 ctaatacatc tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca
    20701 agaaggcgag aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa
    20761 aaacaaattc acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga
    20821 aatgtgtcat tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc
    20881 agctgcctct ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt
    20941 aattgaaaac aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat
    21001 attcagcaag gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta
    21061 tgctatactt ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac
    21121 aactagaaca aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc
    21181 tggatacaaa acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta
    21241 atcacaagat tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc
    21301 aacatgagca ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc
    21361 aaacgttgtc tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc
    21421 gtgaaacaga aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa
    21481 tcgttattga ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg
    21541 cattttatga aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta
    21601 aaatccaaga aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact
    21661 ttttgctaga gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga
    21721 tacaaaaagc acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag
    21781 gaatcgccgg aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc
    21841 ataatcgcaa gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt
    21901 ctgattacat ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat
    21961 tcaaaaatta tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag
    22021 aaagtggttt tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct
    22081 tagtcgatca gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg
    22141 atatgaaaat aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag
    22201 aaacaaatga acaattacct ttacatcata gactttctct attacaaacg aagctcctga
    22261 aaaaattagg cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg
    22321 aagaacagct ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg
    22381 agcgttcatt acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc
    22441 atcgagggat catgaattat caatttgtca atataacgaa acaatatctc cactttctac
    22501 aaactgtgcc actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac
    22561 atttgacaac tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac
    22621 tattcttttt actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata
    22681 tctttattga tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt
    22741 tcccaaaagc aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa
    22801 cgatcgttgg atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa
    22861 cgacaagcta tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag
    22921 aacatcaagt agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa
    22981 cagatgatat caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc
    23041 ggtattggcg tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc
    23101 aactgccaga cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga
    23161 agcgttccat tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt
    23221 tcttgtggga tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca
    23281 ccatgtgtac acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt
    23341 tgttaaacaa tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac
    23401 tgctatcatt aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa
    23461 ttcaatcatt taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta
    23521 atttgacaag cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa
    23581 ataccttgct atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt
    23641 caatgaattg gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct
    23701 ctgatacacg aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct
    23761 tctcatttct ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta
    23821 gaaagaaagc ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac
    23881 gaaaaagcag cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca
    23941 tacactctaa ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt
    24001 caacgccgaa tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta
    24061 actcttctac tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg
    24121 cttccatatt cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat
    24181 gctctcatca taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac
    24241 aatctgttag tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg
    24301 gttgcttagt cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc
    24361 agtgcatcaa tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt
    24421 tgagggatca tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct
    24481 tcttgtatac tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc
    24541 attacatcgc gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct
    24601 tctcctgcaa aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca
    24661 cccatgttcc cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca
    24721 ttgttcgtca atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc
    24781 aattcagagt atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata
    24841 tgttttggta cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca
    24901 tgcggatcag gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa
    24961 atccggctat tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt
    25021 cccatatttt cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc
    25081 attatacttg tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca
    25141 tttttagtca attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt
    25201 gcctcccaat tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt
    25261 ttttccgatt cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc
    25321 atcggtaaat ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt
    25381 tttgatattc agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt
    25441 tgtttttacc tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat
    25501 gcgcgattct attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga
    25561 taacaaccga taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga
    25621 aagatcttct tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc
    25681 atggcgaatg gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc
    25741 tgcactttta cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt
    25801 tgcataactt aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata
    25861 cagactgcga aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc
    25921 ttgaaatgcc tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta
    25981 atcaaacgaa gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa
    26041 gatttttact gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag
    26101 ctccactact aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat
    26161 gatagccggt tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc
    26221 tcctgttatt tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta
    26281 ctctacgata atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag
    26341 aaatagagac aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat
    26401 tctcgcaaac acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg
    26461 ttcggatata taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa
    26521 ccgctagtct ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc
    26581 tgtaaaatca gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata
    26641 actttccaat ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga
    26701 cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc
    26761 tggataacta tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg
    26821 aaaaacctca tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc
    26881 taacgcttga tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca
    26941 cagcgaatca gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat
    27001 attgccttcc acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat
    27061 caacatctct tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac
    27121 cgctaatgct ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg
    27181 acgtttcgaa aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat
    27241 gaaagcaaat gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg
    27301 agcatctgac catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg
    27361 tacgatcaca tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg
    27421 ttttacttca tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat
    27481 ttgacgttcg ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg
    27541 aacgtctaaa gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt
    27601 taattgttct ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct
    27661 acccagtaat tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa
    27721 tgggccaatg tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg
    27781 gctcgtgcat cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa
    27841 atgattttgt caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga
    27901 agaatctctg catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca
    27961 tgccagattg cattattata attcaataac tccggatcaa aaccgtctct tacgattacc
    28021 attttgaact cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta
    28081 gctttttcag gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca
    28141 agcacatgct tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct
    28201 ctagaataag cagtatattc attactatag tccatattca tacaaccctt cattttgaat
    28261 ataatcaatt actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg
    28321 acgtattttc gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg
    28381 agtatccgtt gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc
    28441 atcgatttta taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata
    28501 atctgtatcc ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc
    28561 accacgagct aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc
    28621 caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg
    28681 ataagaaggc atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat
    28741 cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt
    28801 cttctctaag aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc
    28861 ttgcgtattc atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata
    28921 cttttctttt gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc
    28981 gcaatctatt tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg
    29041 aagtaacgat actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat
    29101 tgctgaattg atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct
    29161 acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa
    29221 tgctttgcgt ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc
    29281 tgttacagtg atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa
    29341 ctcaaaacga acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa
    29401 tccacctaca tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt
    29461 tacatcattt gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct
    29521 tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgtt
    29581 ttcttttaac ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg
    29641 gcgatggata attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat
    29701 tttatccaag gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt
    29761 atttttgatg atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata
    29821 gacatcccgt ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga
    29881 tttcttagca ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc
    29941 cttcatccat tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt
    30001 cccaactaaa agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc
    30061 attgaagtca aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt
    30121 cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct tgggtctcat tataatggcg
    30181 taatctgaaa caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt
    30241 ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat
    30301 acattgtaat tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct
    30361 aatagatagg ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct
    30421 gttgcgataa tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata
    30481 tcagtcataa tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc
    30541 tttgctatgt tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca
    30601 aatttttcaa tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg
    30661 attcctgcat ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta
    30721 ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt
    30781 aattgggcag gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa
    30841 aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg
    30901 tattatactg catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag
    30961 catttcttta caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa
    31021 acaactaata ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc
    31081 gaacaagttc cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc
    31141 gtggcaactg ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc
    31201 gtagttctgg cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt
    31261 tccacaaaat cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca
    31321 tccagacttc atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga
    31381 tgatggcgat gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct
    31441 ctccttcgcc gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg
    31501 taatcaccgg tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc
    31561 cttcaggatg gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt
    31621 gaataccgat cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt
    31681 cgtcatctgc aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa
    31741 aaaaatccaa gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg
    31801 tcttatccac gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc
    31861 tgtttcaatt ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca
    31921 gccttttgga aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca
    31981 gcaaaaccga tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg
    32041 gcagttactc ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg
    32101 ctatgccgtt tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct
    32161 tgcatccgtg cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct
    32221 cgttcgaata atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa
    32281 ttaggatcca tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg
    32341 actgcttcat gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga
    32401 aatgctagcg gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca
    32461 ataatcaaag ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat
    32521 actttttctt caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc
    32581 cacatgttat caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc
    32641 cgaatatatt tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt
    32701 gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact
    32761 gcaggatgac taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata
    32821 aattcactta aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg
    32881 attatttttg caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca
    32941 ctgtctactc gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga
    33001 gaaggagcaa aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct
    33061 tggaatacta cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt
    33121 ttttttacta aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga
    33181 gtattcattt ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg
    33241 gcattttcat aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt
    33301 tttgtttgtg agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt
    33361 aaagaacaat ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga
    33421 acttctatat ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat
    33481 gccgctgctg cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga
    33541 actttccgaa tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca
    33601 attctgtctg ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga
    33661 atgactggga catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca
    33721 tcgatcgttg cacttttagg accaataaat gtgatattgc actcttcaca catggaggca
    33781 aatcggctat tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg
    33841 attgcagcgc tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca
    33901 atgcagattg cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat
    33961 atcgctacgg tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca
    34021 ccgcggttag ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa
    34081 ttccgcttcg gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga
    34141 agctttcact ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt
    34201 acggaatttt gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag
    34261 agaaagtaat gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat
    34321 aacaggttct tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt
    34381 agcaacgatc cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata
    34441 acgatgtgga atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt
    34501 tgcttatttt tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc
    34561 tacaaggatt tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat
    34621 cgcttcaata atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc
    34681 tggtttgtcg ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat
    34741 tccatcaaat actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc
    34801 aggtatgaca ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat
    34861 gcctcgactt gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa
    34921 ttccgttagc gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa
    34981 aattattcct cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa
    35041 ttactcattg cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca
    35101 atctcaggat cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc
    35161 agcaaagttg ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat
    35221 ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt
    35281 gcttctgcct ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt
    35341 tccggctgta caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca
    35401 tctggagtag gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct
    35461 ccatagattt tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct
    35521 gctccttcac ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta
    35581 ggatcagttg attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa
    35641 atcgttgctt ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg
    35701 aatgcttccc caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata
    35761 ccttttgcac ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt
    35821 acgaacattg gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg
    35881 attgttggaa gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt
    35941 ttttctttat ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc
    36001 gaaaacatat ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct
    36061 ttgacttcac ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg
    36121 atgccattcg tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt
    36181 gaagtgactc catagccagt aattaccact cgattcattt gtttgctctc cttttcttat
    36241 ccgtgcatga ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt
    36301 gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt
    36361 tgcttttccg cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg
    36421 aatcctggcg caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat
    36481 tttgttaatc ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca
    36541 atcaacccag atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt
    36601 ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg
    36661 aaatcttctg catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact
    36721 aacacatgga tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca
    36781 taatcagaga tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct
    36841 atcttttctt ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca
    36901 aaagctaagg ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt
    36961 ccttttacat ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct
    37021 tattctcaac acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata
    37081 cttttccggg gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac
    37141 tctcatagaa acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt
    37201 tcttcattat ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa
    37261 tctgacttag gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag
    37321 gcccgctgac atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag
    37381 cttcgtctac tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg
    37441 caggagatac gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg
    37501 gcgcattcat cactgcaacc attttcccgc ttccagctgg ggcagcttct gtcatataag
    37561 cgcctctttt tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta
    37621 atgccgaata ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct
    37681 tcaataagcg ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct
    37741 gatccaaacg ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac
    37801 ttgcttcatc aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt
    37861 gatattgtgc accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat
    37921 ccagaatata tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa
    37981 tgtcccagcc cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact
    38041 aaatcttgaa ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca
    38101 atttcattga tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca
    38161 gtagtcaatt tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt
    38221 ttttcgaata ccataataat atattcctcc gatattgttt gctttttatt tttataattt
    38281 aacagtctac aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa
    38341 tattactttc tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa
    38401 gatagggatg cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc
    38461 ttgcggcatc tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa
    38521 aagcagataa tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg
    38581 tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc
    38641 ttctgaatag aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt
    38701 accgtcagct gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat
    38761 cccacctgct ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt
    38821 agatagtgtt tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc
    38881 agccatgctt aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc
    38941 atgagttgct cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg
    39001 cgacattgtt gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat
    39061 caatttttcc gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg
    39121 ttcacgaata cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag
    39181 atcattatta gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa
    39241 ttgttccatt aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc
    39301 atccagcaag gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc
    39361 acgatgaaaa tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt
    39421 tacaacgcgg cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt
    39481 gttaatagca gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt
    39541 tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc
    39601 tttgaattga gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact
    39661 aacaagataa tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag
    39721 tttgattatc aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct
    39781 taaaagatca tttttttact atatgagctt tttttaagat tttactcatt taaaacaact
    39841 gataaatcag catttataat ttcttttatt agaaaaatca tctatctcac atattttgat
    39901 tatcaaagta ttttctgtga acagaactat agaattccct aaaacttgtc cattatttca
    39961 aaatttgtta tagtcttttt attcaaagcg taaaaggaga tcctatgtta aaagaaaaaa
    40021 tgaaaactct ccccatgctg ccgggtgtct atcttatgaa agaccgagat ggtaaggtca
    40081 tctatgttgg taaagcaaaa cggctcaaaa atagagtaag cagctatttt catcagaata
    40141 aacagcattc aaaaaaagta ttacgtatga ttcatcatat aacagatttt gatttcgtcg
    40201 tagtagacac ggaattagat gctttattgc tcgagtgtca attgatccaa cattaccgtc
    40261 ctttttataa ccgacaaatg aattatttca gtaattataa ttatgtacat attaccaata
    40321 agggatttgt attaacagat actcctacgg ctcgaacata cgggcctttt cgtctctata
    40381 aaaaaatgcc gtctattttg cgtataatgg aagaaactta tcaaatgcct tggttgtctg
    40441 aaattagttt gttagctttg cgagtccagc ttcctgacct acaggaaatg acttttgaac
    40501 agaaaaaaaa ggaactccaa gggctgtttc aaggccgaaa taaaaagcta ctgacttact
    40561 tgaaaaaaag acaacaacat ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc
    40621 aaaaagatat cgaattagtt acttatttta ttagacggat ccaggaacaa aagcagtttt
    40681 taagaacacc atcgctcacc ttcagcatgc cattagcagc agatgagagt cagaaaaagc
    40741 actacctcgt ttgttatggt caacttgcag aaacaatgat tacctctggt gacgtttctc
    40801 ctgacttcta ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag
    40861 aagaaatcga tcctgtacaa atcttgatca gttaccgcaa aaaattagaa aaagaacagt
    40921 ttgaaattga actgttaaac aaaaaagaag ctgaaaagca actaaattag ttgcttctcg
    40981 gcttctttta ggcatcttca acttcaaatg tcaattatcc taaaatttat tggattaagt
    41041 catagatttc catcgctacg atatcgatat tatcaaattc gtattgctga tgagtatctg
    41101 tttcagttaa ttcaaatgta tcggtcgctt tatcataagt tactgtacac ttctctacgc
    41161 cttctttttc aaaacggcga acttgcagtt cattatctgc tgactcgacc atagcctcca
    41221 gtcgttttat gattgcgact aattgtgaat gtgtcatcgg aggtcctcct tttcttattt
    41281 ctgttatatt gtatcaaaag catagcctac ttgcattgat tttctctaaa aaaactcgtt
    41341 ttttatgatt ttttttctgg tatttttcct tcaggccacc aaagtttgat caaagcttgg
    41401 gttccgtcat tctcaaaacc tttatctgcc aactcttcat atagtttcaa tgcttgggta
    41461 gttgctggca atacaagatc cattttttct gcttcttcta atgcaatctt taaatctttg
    41521 ataaaatgtt tgacaaaaaa accaggtgtg taatcttctt ttaaaatacg aggagaataa
    41581 ttgcttaaag accagttagc tgcacttcca cctgaaagcg tctcaatcac tttctttaaa
    41641 tcaagtccgt ttttttgtgc atagacaagc atttccgtca ttcctgtcat cgtacctgca
    41701 atcatgatct gattagccat ttttgtatgc tgacctttcc cagcagaacc atgcaatgta
    41761 aatgtcgtac caaattcttt gaataaaggc agtacttgat cataaacggc ttcttctcca
    41821 ccgaccatga tcgtcagtgt accattcttt gctcctaagt caccaccgga taccggtgca
    41881 tctaacgcat ctgcgccttt ttcttttgct gtttttgcaa ttttttctgc caggcttggt
    41941 gtacttgtag tcaaatcaac taaaatatgt cctgagatat ctgcagaaaa aatccccgac
    42001 tgtccatagt agatttcttc tacatctttg gggtaaccta ccattgtaaa gagaatatta
    42061 ctttgttctg ctaccgcttt tggtgtatct gcccagatag ctccttctgt tactaaagga
    42121 tcagcttttg cttttgtccg attatagaca gctacttcgt gtcctgcttc tagtagatgg
    42181 cgtgcgacag cacttcccat gacgcctgtt ccgataaaac caagtttcat tttttcgacc
    42241 tcctaaaaat caaaaatcct tgcttctatg ctagtatacg ctagaaacaa ggattttttc
    42301 ataaaaaatg tttgaatttt atcttttaaa tgaatttaat tttattttga ttcgttcaat
    42361 cgagtcttct tcttcttttc ttcgcatctc ttcttcatga taaacaacca tgtcttgata
    42421 aaattcgatc atagcttcgc cgaaatgtgt agatgaaata tcataaagtt tagcttctaa
    42481 aacttttgga tctttaggaa cttcactgga aatataatca ataagtgctg gtgcaaagtc
    42541 ttcgtcggtt tcaaaagttt tccctaatga aggatgatcg aacaatcggt tcaaataagc
    42601 atttccttcc acgacacaag gtgttccggc tgccatcgct tctgtataag taagcccttg
    42661 tgtttctgaa gtagaagcac tgacaaaata atctgccgct ttataataaa gtgctacatc
    42721 attgttgtcc acttctcctg tgaactgcac aaattcttct aactgcagtt ctgagatcaa
    42781 ttcctctaac tcttctacat aaggtccttt tcctacgata actaatctgg cgttaggata
    42841 agcggagatg acctgaggaa acccattaat caaggcttgg atatttttct cataagaaat
    42901 cctgcttaaa gaaagaatca tcaaatggtt ttcctgaaga cctagtgact cccttaattt
    42961 gctgatatcc tgctgagtga tatcagaacg tttgaatttt tcaatgtcga taccagtagg
    43021 aatcacgcga agcggtgcta ttacaccata gctgcgtaat gtatcaataa cacgttcact
    43081 tggacaaact acacctgtcg tatgatttgc aaataatcta gacaagtatt ttacatggga
    43141 ttgcctgatc acttttcctt taccgatata atgaaggtag tcttcataca tcgtatggta
    43201 agtatggatc agaggtattt tcaacttctt agctaccatc ttcccaagta tccctgctcc
    43261 gaattctgta tgggtatgaa taagatctag ttctagttct ttggcaacca aataagcata
    43321 ccacatgcct ctaacaacaa ctcttcgatc cttgaaagaa ataaatggta cacttggcat
    43381 tcgaatcaca tctttttcca acttatccgc attaggatcg gtcgttgtaa aaatatagac
    43441 ttcatgccca tgtctttcta attcttgttt taatgttttg atcgatgtag caactccgct
    43501 gacttgcgga aagtaggtat cggtaaaaaa gccgattttc atgtttttcg cctccaaact
    43561 agtccttgac ctgatttact ttttttaatt tgctagagac tttttcattc agtacacttt
    43621 cgtatacatt tttaagttct tcaccgattt tattgatact tcgctctttt gctgtttgat
    43681 aaccggcttg tgtcgtactc ggtagttctt gattggtaat ctgttggatc agtcggataa
    43741 attcttcgtt tgtcttcccc atatagcagt tcttgctatc ttctaaccag ccattataca
    43801 ctggaatatc tcgaacaagt acgttttgtt tgcttgctaa agcttctaaa acaacgatcc
    43861 cttctgtctc ttcataagaa gggaaaaaga atagatctgc acctgaatac gcaccttcta
    43921 tgatctctcc acgaatgtaa ccaggaaaca ggacattttc aggatgatct tcttttacaa
    43981 ttttccggat gttccgagga atcgagtaca tgggaacatg accaaaccaa ataaatgtgt
    44041 attcgggcat cttcttagca atttctacga aatcaatgat tcctttacgt tcgaaaaaca
    44101 aaccgacaca aatgattact ttttgatccg gagacagttt gaaatattct ctgaactttt
    44161 gttctttcat tggatcaggg tgatatttct ctaagtcgat cccattggaa atagcttgaa
    44221 tcggcacacg gataccatag ctttccaaca actgttttga ataaggtgtt ggcgtgatca
    44281 gatagtccgc tttttggtac aatcctacca gatatttttt tacgatagga gaaagctggt
    44341 tcgatccgat aaaggaattt cggaaatctt cctctgtaga atgcgcatga tagacaactt
    44401 tttttccttc tcttctggct ttattgacca tattatgact attgattccg tatgtattga
    44461 tgtgcaggat gtcataatct tctttttcat ctagtgtata agagataccg acttccgtca
    44521 gagcacgttt ttgatgatca agcgcacgcc cgattccaga ttttgcaagc atcttttcac
    44581 tttcaaaata taataatacc ttcaccttac gtcccccttc acttatatag tataacatag
    44641 cctacaaact gtcactcaaa cttaagccct atttttgtct attcttactg acaaaaacgt
    44701 tttcttaaaa aagaacaaaa aaactggcgt agctctttta caagccacac cagcttttga
    44761 tacgttgttt attttacata ttcatgaaca agttcaacga cttcttccat cgtatcgcat
    44821 tctttcaatg cacgatcagc aagttcagcc attttcgctg tgtccaagcg tttcattaaa
    44881 ctacgcgttt taagaataga tgtcgcactc attgagaact catctagacc catacctact
    44941 agcaatggaa cagctgtttg atctccagcc atttctccac acatacctgc ccattttcct
    45001 tcagcgtgtg ctgcatcaat tacatttttg atcaaacgta aaattgatgg gttatatggt
    45061 tggtacaagt aagaaacgcg ttcgttcata cggtctgctg ccattgtgta ttgaatcaaa
    45121 tcgttcgtac ctacagagaa gaaatctact tcttttgcaa acttgtctgc taagactgct
    45181 gctgctggaa tctcgatcat gatgccgact tggatcgtat cagatacttc cttgccttca
    45241 cttatcaatt tttgtttttc ttcttcaaag atcgcttttg ctgcgcggaa ttctttgagt
    45301 gtagctacca tagggaacat gatacgtaaa ttaccatgaa cagatgcacg aagcaatgca
    45361 cgcatttgtg tacggaacat ttcgtcgcct tgttcagata aactgatacg caatgcacga
    45421 tatcctaaga atggattcat ttcatgaggt aattgcagat atggtagttc tttatctcca
    45481 ccgatgtcca ttgtacgaac aactacaggt tttccttcca taccttctaa tacagctttg
    45541 taagcttcat attggtcatc ttctgttggg aagtctggag aatccatata caggaattca
    45601 gtacggtaca gaccaactgc ttctccacca ttattatgga caccaactag atcttttggt
    45661 gttccgatgt ttgcagctaa ttcaaaatgc ttgccgtcag ctgtgacagt ttctgcattt
    45721 tttaattttt cccattctgc tttttgtgct gcgtaatctg cacctgcttt ttcaaattca
    45781 gctttttgtt catctgtagg atcgatgatt acatcgcctt cgatcccgtt gacagccaaa
    45841 atatcgcctt ctttgacttt agcagtaatt tcttttgtcc caacgattgc tggaatttct
    45901 aaagaacgtg ccatgatagc agaatgtgaa gtacgtccac caatgtctgt tacaaacgct
    45961 tttacaaaat tgcgatctaa ttgtgctgta tcacttggag tcaagtcatg tgcaacaacg
    46021 accacttctt cgttgatcat tgatggattt ggtaaagtaa cgcctaataa gtgcgctaaa
    46081 atacgtttag caacgtcacg aatatctgca gcacgctctt gcatataagc attatcttcc
    46141 attgcttcga acataccgat atacatatcc gttacttctt ttagcgctga ttcagcattt
    46201 acgctgttgt ctttaatatt ttgcttgatt tgacctacca tttctggatc tgaaagaacc
    46261 atcaaatggg catcaaatac ttgcgcttct gcttcaccta agctttgcgc tgctttttca
    46321 cgaatttgct gcaattcttc agtagattta gctaatgcag catccaaacg agcttcttca
    46381 gccgcagtat cttctactgt tgccttactg aatgttaaat ccggttgaac tagcaggtaa
    46441 gcttttgcaa cggctacacc gtcactagcg gcaatccctt ttagcatttc aaccattatt
    46501 cagataagcc ttctttcttc attgtttcaa cgattgctgc cattgcatca gcttcgtcag
    46561 caccttctgc agtaatagta acatcagaac cttgacctac acctaaagac ataacgccca
    46621 tgattgattt caagtttact gatttacctt tatactctaa gtttacatca gagttaaatt
    46681 tgcttgctgt ttgtactaat agtgtagctg gacgagcgtg gatcccagtt tctgctacta
    46741 cgtgaaattc tttcttttcc atattaatcg gtctccttta agtgagtgtt gtttttatgt
    46801 gagggttttc ttttttagta attcagagaa tcctaacaaa tgccaccctt tcatctgtaa
    46861 agattaacat tttttttcgt tagatacaac agttttcatt caatttcaga taaaagtatt
    46921 caaattattc cggattcttt ttcatccttt tcttcacatt ttataacaaa cgtgtttatt
    46981 tcctttttct tgtcgctttc tcttctcctg acgtttatca ttctttcctt tgaccctctt
    47041 tgttttcata tgaaacgaat tacttgcttc cctttttatt tttagtataa taagagtcaa
    47101 aggtcaggaa tggtcaatag agatttcacc ttttctacct cataaatctc ttaaaaaact
    47161 aggggttatt cttttacctc atgaaaaacg agatgtaaaa tatggatgta cccatttttg
    47221 tgaaaggacg tgaatgattc atgctttgtc aaaactgcgg caagaatgag gcaacgattc
    47281 acttgtatgc cactgtcaat ggtcagcgga cacaactcga ttactgtcag agctgttatc
    47341 aaaaaatcaa aaatcaacaa aatggaggtc tgactaatat ggcccaaaat gatccatttg
    47401 gatttggcag tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa
    47461 attatgagca aacaccacca acacaatttg gcggaaacaa caacttcaat ggtggtcagc
    47521 cacctcaagg caatacagaa ggtttattgg gagaatacgg aatcaatatc acagaacaag
    47581 cacgacaagg tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg
    47641 aaattttaaa tcgccgaacc aagaacaatc ctgtcttgat tggagaacca ggtgttggta
    47701 aaacagcagt tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat
    47761 taatggacaa agaagtcatt cgtttagatg tcgtatcttt agtccaaggt actggtatac
    47821 gaggacaatt tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg
    47881 tgatcctatt catagatgaa gttcacgaaa tagtcggtgc tggttctgct ggtgatggaa
    47941 atatggatgc tggtaatatt ttaaaaccag ctttagctcg aggagaattg caaatggttg
    48001 gtgctactac cttaaatgag taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta
    48061 tgcaacctgt acgggtagat gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt
    48121 tgcaaaaacg ttatgaagac tatcatcatg taaaatatac tgatgaggcc atcgaagcag
    48181 ccgcaacttt atccaatcgt tacgtccaag atcgcttctt gccggataaa gccatcgatt
    48241 tgctagatga aacaggttct aagaaaaact tgacgatcca aatcgttgat cctaaaacaa
    48301 tcgagaaaaa actgcaagaa gcagaagaac aaaaagtttt agcttctcga gaagaagatt
    48361 tcgaaaaagc agcttattat cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc
    48421 agctcactga agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac
    48481 aacggacagg tattccagtg ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact
    48541 tagcagatga cttgaaagct catgtcatcg gacaagataa cgctgtagat cgagtagcga
    48601 aagcgattcg ccgaaaccgt gttggattaa acaaacagaa tcgtccaatc ggctccttct
    48661 tatttgttgg accaactggt gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac
    48721 tattcggttc acaagattca atgattcgtt tcgatatgtc tgaatatatg gaaaaacata
    48781 gtgtctctaa attgattggt tctcctccag gttatgtcgg atacgaagaa gctggtcaat
    48841 taacagaaaa agtacggaga aatccttaca gtcttgtctt gttagatgaa gtagaaaaag
    48901 ctcatccaga tgttttgcat atgttcttgc aaatcttaga tgatggtcga ttgacagatg
    48961 cacaaggaag aacggtcagc tttaaagata cgatcatcat tatgacaagt aatgccggaa
    49021 ctggtaaagt agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt actcgttctg
    49081 tattgaacca attaaacaac tactttacac cagaatttct gaaccgtttt gacgggatca
    49141 tcgaattcag tgcattatca aaagaaaact tgatgacaat cgtcacacta atgctagacg
    49201 atgtcaacca aatgctggca gcacagcaac ttcacatcga agttcctacg aatgtcaaag
    49261 aaaaattagt tgacttaggc tatgatccat ctatgggtgc tcgtcctctt cgacgcacga
    49321 tccaagaaca aatcgaagat ggtatcgctg aattttatct tgatcaccct tctatccatg
    49381 agttgaaagc aaaactggat aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt
    49441 tggctaaaga atcagccgaa gaaacggctg aataaatcca gttttatagc gtgagacggt
    49501 atttctttga gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt
    49561 ataaaaaaga aattataata agagaaacaa gcaattaaaa atacataaaa gaggtgagcg
    49621 ccatgaaatt ggtaaatgta acaaacagcc attcccgtct tgtattaaac cagcttgaaa
    49681 acactgatgc ccatctagtc aaggtctaca cagccggaaa taccactatt gtctacactg
    49741 aagcccctga acataatgaa attctgctga tcaatgacaa acgtaaaatc cagccgaaag
    49801 aaatcgaaga tgctaaaaat tatttcttag ataaaatcaa agacgccgtt taccacgagg
    49861 atgaaatcaa agtaatcgaa ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat
    49921 taggcgctac attagccgtg taaacaaatt ttgaacataa taattaaaga gcgtgggaca
    49981 aaagtaaaga ggctctttgt caaataggac tgatagagtg agataagaac atctaaggta
    50041 gaatt
//
LOCUS       DAFEHS010000012        49882 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFEHS010000012
VERSION     DAFEHS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.012"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..347
                     /locus_tag="LP327_000746"
     CDS             <1..347
                     /locus_tag="LP327_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HBL1724889.1"
                     /translation="SHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRESFGRTKLFLES
                     GLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYIPMFNQQPPTLE
                     EIFKLKAGEIDA"
     gene            340..1602
                     /locus_tag="LP327_000747"
     CDS             340..1602
                     /locus_tag="LP327_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1724890.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            1765..3663
                     /locus_tag="LP327_000748"
     CDS             1765..3663
                     /locus_tag="LP327_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBL1724891.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(3962..4681)
                     /locus_tag="LP327_000749"
     CDS             complement(3962..4681)
                     /locus_tag="LP327_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBL1724892.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            5018..5350
                     /locus_tag="LP327_000750"
     CDS             5018..5350
                     /locus_tag="LP327_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724893.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            5337..7340
                     /locus_tag="LP327_000751"
     CDS             5337..7340
                     /locus_tag="LP327_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBL1724894.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            7359..7829
                     /locus_tag="LP327_000752"
     CDS             7359..7829
                     /locus_tag="LP327_000752"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBL1724895.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            7850..8434
                     /locus_tag="LP327_000753"
     CDS             7850..8434
                     /locus_tag="LP327_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBL1724896.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            8449..9435
                     /locus_tag="LP327_000754"
     CDS             8449..9435
                     /locus_tag="LP327_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBL1724897.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            9425..9736
                     /locus_tag="LP327_000755"
     CDS             9425..9736
                     /locus_tag="LP327_000755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBL1724898.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            9758..11539
                     /locus_tag="LP327_000756"
     CDS             9758..11539
                     /locus_tag="LP327_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBL1724899.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            11532..12908
                     /locus_tag="LP327_000757"
     CDS             11532..12908
                     /locus_tag="LP327_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBL1724900.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            12915..13550
                     /locus_tag="LP327_000758"
     CDS             12915..13550
                     /locus_tag="LP327_000758"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBL1724901.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            13580..14935
                     /locus_tag="LP327_000759"
     CDS             13580..14935
                     /locus_tag="LP327_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBL1724902.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(15127..16521)
                     /locus_tag="LP327_000760"
     CDS             complement(15127..16521)
                     /locus_tag="LP327_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL1724903.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            16613..17584
                     /locus_tag="LP327_000761"
     CDS             16613..17584
                     /locus_tag="LP327_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBL1724904.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17581..18363
                     /locus_tag="LP327_000762"
     CDS             17581..18363
                     /locus_tag="LP327_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBL1724905.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(18532..18903)
                     /locus_tag="LP327_000763"
     CDS             complement(18532..18903)
                     /locus_tag="LP327_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL1724906.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            19037..19480
                     /locus_tag="LP327_000764"
     CDS             19037..19480
                     /locus_tag="LP327_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBL1724907.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(19523..20332)
                     /gene="yidA"
                     /locus_tag="LP327_000765"
     CDS             complement(19523..20332)
                     /gene="yidA"
                     /locus_tag="LP327_000765"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBL1724908.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(20351..21718)
                     /locus_tag="LP327_000766"
     CDS             complement(20351..21718)
                     /locus_tag="LP327_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL1724909.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(21702..22532)
                     /locus_tag="LP327_000767"
     CDS             complement(21702..22532)
                     /locus_tag="LP327_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBL1724910.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            22695..23132
                     /locus_tag="LP327_000768"
     CDS             22695..23132
                     /locus_tag="LP327_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBL1724911.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            23208..23828
                     /locus_tag="LP327_000769"
     CDS             23208..23828
                     /locus_tag="LP327_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBL1724912.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            24017..24523
                     /locus_tag="LP327_000770"
     CDS             24017..24523
                     /locus_tag="LP327_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBL1724913.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            24540..25439
                     /locus_tag="LP327_000771"
     CDS             24540..25439
                     /locus_tag="LP327_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBL1724914.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            25526..26410
                     /locus_tag="LP327_000772"
     CDS             25526..26410
                     /locus_tag="LP327_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL1724915.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(26602..26673)
                     /locus_tag="LP327_000773"
     tRNA            complement(26602..26673)
                     /locus_tag="LP327_000773"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26638..26640),aa:Arg,seq:ccg)
     gene            26733..28067
                     /gene="rpoN"
                     /locus_tag="LP327_000774"
     CDS             26733..28067
                     /gene="rpoN"
                     /locus_tag="LP327_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBL1724916.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            28216..29307
                     /locus_tag="LP327_000775"
     CDS             28216..29307
                     /locus_tag="LP327_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBL1724917.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            29348..30349
                     /gene="gap"
                     /locus_tag="LP327_000776"
     CDS             29348..30349
                     /gene="gap"
                     /locus_tag="LP327_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL1724918.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            30459..31649
                     /locus_tag="LP327_000777"
     CDS             30459..31649
                     /locus_tag="LP327_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBL1724919.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            31753..32508
                     /locus_tag="LP327_000778"
     CDS             31753..32508
                     /locus_tag="LP327_000778"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBL1724920.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            32624..33922
                     /gene="eno"
                     /locus_tag="LP327_000779"
     CDS             32624..33922
                     /gene="eno"
                     /locus_tag="LP327_000779"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBL1724921.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            34322..35461
                     /locus_tag="LP327_000780"
     CDS             34322..35461
                     /locus_tag="LP327_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBL1724922.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(35528..36103)
                     /locus_tag="LP327_000781"
     CDS             complement(35528..36103)
                     /locus_tag="LP327_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL1724923.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(36108..37082)
                     /locus_tag="LP327_000782"
     CDS             complement(36108..37082)
                     /locus_tag="LP327_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBL1724924.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(37106..37150)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            37365..38333
                     /locus_tag="LP327_000783"
     CDS             37365..38333
                     /locus_tag="LP327_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL1724925.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            38446..38724
                     /locus_tag="LP327_000784"
     CDS             38446..38724
                     /locus_tag="LP327_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL1724926.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            38842..39078
                     /gene="secG"
                     /locus_tag="LP327_000785"
     CDS             38842..39078
                     /gene="secG"
                     /locus_tag="LP327_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBL1724927.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            39181..39936
                     /locus_tag="LP327_000786"
     CDS             39181..39936
                     /locus_tag="LP327_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL1724928.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            39939..42296
                     /gene="rnr"
                     /locus_tag="LP327_000787"
     CDS             39939..42296
                     /gene="rnr"
                     /locus_tag="LP327_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBL1724929.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            42318..42782
                     /gene="smpB"
                     /locus_tag="LP327_000788"
     CDS             42318..42782
                     /gene="smpB"
                     /locus_tag="LP327_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBL1724930.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            43084..43803
                     /gene="atpB"
                     /locus_tag="LP327_000789"
     CDS             43084..43803
                     /gene="atpB"
                     /locus_tag="LP327_000789"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBL1724931.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            43856..44071
                     /gene="atpE"
                     /locus_tag="LP327_000790"
     CDS             43856..44071
                     /gene="atpE"
                     /locus_tag="LP327_000790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBL1724932.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            44162..44686
                     /gene="atpF"
                     /locus_tag="LP327_000791"
     CDS             44162..44686
                     /gene="atpF"
                     /locus_tag="LP327_000791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBL1724933.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            44673..45215
                     /locus_tag="LP327_000792"
     CDS             44673..45215
                     /locus_tag="LP327_000792"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBL1724934.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            45238..46794
                     /locus_tag="LP327_000793"
     CDS             45238..46794
                     /locus_tag="LP327_000793"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBL1724935.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            46808..47710
                     /locus_tag="LP327_000794"
     CDS             46808..47710
                     /locus_tag="LP327_000794"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBL1724936.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            47733..49139
                     /gene="atpD"
                     /locus_tag="LP327_000795"
     CDS             47733..49139
                     /gene="atpD"
                     /locus_tag="LP327_000795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBL1724937.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            49153..49575
                     /locus_tag="LP327_000796"
     CDS             49153..49575
                     /locus_tag="LP327_000796"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBL1724938.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        16496 a         8832 c        10648 g        13906 t
ORIGIN      
        1 ctagccacaa tatggataat gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg
       61 gaaaaatggt tttgaatgga aaagtacatg agattcgtga atcatttggc cgtacgaagt
      121 tattcttgga atctggttta agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag
      181 tgatccttca tgaagacaat acgttagaga ttaccttaga agatcctgaa gctgggaaag
      241 aaatctttac tcgtgcgact cagttcggct acattcctat gtttaaccag caaccgccaa
      301 ctttggaaga gattttcaaa ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt
      361 accaaagatg tctatttaaa aaatgttaag tcgatttctt tcttgatcat gatattagtc
      421 ccttttatca tgctgggtat catttatgta gcaggaaact ttgctcaaca aaatagtgat
      481 gtcaataaaa tcggcgtatt ggctgataat gagcaaatca gtcagcaaat gagccaagtc
      541 aaaacagatg attttgagtt caaagcagtt gattctgaag aaaaagcaca aaaagagctt
      601 tcggaagaaa aaatcgatgc gtatttagta gtctcaactg agaatgataa gataaatggg
      661 atgctttata gcgaaaattc attaggacaa agtacacaac tactgataca acaacaactg
      721 actggcttgc aatcgatgct tcgagcaagt caattaggca tttcaccaga agaagtagct
      781 agcttgagcc aacaagcaag tttgactcga caaaaagtca attttgattc aaaaggaaaa
      841 atgacagttg gagaagataa ctcgacagtc caatatgtcg taagttatgt agcaacgatt
      901 gtcttgttca ttattatttt gacgtatgcg cagatcattg cacaagagat cgcttctgaa
      961 aaaggcacgc ggatcatgga agtgatcctg tccagtacga ctgcacaaaa gcatttttac
     1021 ggaaaactaa caggtgtttt acttgttgca ttaacgcaaa tggcattata cggtattatt
     1081 tttggtatag gatacagtca attcaaagat atggaaatgg tcaaaaatat attagacggt
     1141 atctcattgg ataacttgat tggaccattc ttgtggtatt ctcttctatt catgatcttt
     1201 ggcattttga ttttttctgt tttagcagct ttatgtggct ctttggtcaa caaagcagaa
     1261 gatacagcaa aagcaatttt gccagtgact tatctatctc taggcggata tatgcttgga
     1321 ttgatactag gagcttcaga tccaaacaat atcgttattc ggatcacttc gtatattcca
     1381 ttcttatctt catatattat gccggttcgc ttagcaaatg agacagtcgg tatgggtgga
     1441 gcattgattt ccttgttggt acttgtagcc gtaacattct tattgatgat cttgtctgca
     1501 aacatgtaca aatcgaatgt actcgtgtat agcgaaggcg gattatggag ttcattgaaa
     1561 caatcgattt caattatgag aaatgaacga aaaaaagcat aaaatataga aaaatataga
     1621 aggaagctgg acatttattg ttcccagctt cttttttgta tagacctatt tctatttttt
     1681 tattatattg gatatatata ttcattgaaa atagagcata acaactttat aatgttgagt
     1741 ataccaattt ttggaggcgt tttaatggac cgctatttag atttactttc agagaagttc
     1801 cctacaacgg aagatgtcat cacggaaatc atcaatctcg aagcaatttt gcagcttcca
     1861 aaaggaacag agttattttt gagcgatata catggcgaat ttcctgcttt tgatcatatt
     1921 ctgcgtatcg gttcaggaaa tcttaaagaa aagatcaaag accttttctc tgaacaaatg
     1981 acggaaacgg agatcagcca attcactatt tttacggctt atccagaata tgtgttgacg
     2041 actgattggt acaaaaaaca agataaatcc cagctgatcc atcgtttgat cgatctttta
     2101 cggtttacga cagtcaaata cactcgatca aaagttcgaa aaggattgcc gaaagaatat
     2161 agctatatca ttgaggaact cttgtatatc gatgatcgta tcaacgggaa aaaagactat
     2221 gtaaaaaaaa tcattgaaca acttgtcttg atgaaggaag aagaaaaatt tttaacaaaa
     2281 cttgcgcaga cgatccaaaa atctgtcatc gatcatttgc atatcgtggg tgatattttt
     2341 gatcgaggaa ctcaagcagc caaagtaatg gatcgaatca tggatttttc ctctgtagat
     2401 atccagtggg ggaatcatga tattttgtgg attggtgcgt attgcggctc tgaggcatgt
     2461 ttattgacgc ttttacggat tgccgcacgc tacaactatt tatttgaatt agagaaagaa
     2521 tatggattaa atttacgtcc attattttct tttgcccatc aaacctatca gaagaatcca
     2581 gtatttaccc ctaaaaacag gaaagatcta tctgaaagag agcaagaaga attagaaaag
     2641 atccatcaag cactttctat cctccaattc aaatcagaac atcagttgtt gaatcgacga
     2701 cctgaattta aaatggacga tcgtaaattg ttggaaaaaa tcaactatga ggaatcaacg
     2761 atcgttttag aaggtcagct ctatgagttg aaagggactt gttttcaaac gatccagcca
     2821 gatgatccta aaaaattctt ggaagaggaa caaaaagtga tcgatagttt gatgtattca
     2881 ttccagcact cgttaagaat gcaaaaacac atgacgtttt tgatcgaaaa agggggaatg
     2941 tatctcacga acaatcagca tgtactgttc catggctgta tacctgtaga tgaaaagggg
     3001 cagctcttag catttgaatt agaccaatct tatcgcggaa aagaactgct gggatttttt
     3061 gaaaaacaga taactgaaag cgcaaaagat ttgactgcaa atgaagattt ggcgaccgat
     3121 ttgatttggt atgcgtggag cgggccattt tcgcctttat tcggcaagag taaaatggca
     3181 acttttgagc gctattttct cacagagcct catacgcacg tcgaaaaaag caatccctat
     3241 tatcacttgc gagatgaaga gtggttcagt gaaaaaattt tggcagaatt cgatgcaaaa
     3301 gaagaaggtt cagcaatcat caatggacac acgccagtaa aagtaaaaaa aggagagtca
     3361 ccgatcaaag caaacggaaa agcatttgtt attgacggag gtctatccaa agcttatcaa
     3421 aaaacaaccg ggattgctgg ctactcatta ctatgcaatt cttacggttt ccaaatcgtg
     3481 acacattatc cgttcttgcc tattgaacaa caatttgcaa aaaaatctga tcaaacgaat
     3541 gtaaaaaaag tgatcgagca gcagctccca cgcaaactta atcggaatac gacaaaagga
     3601 atggaattac agcaacagat tactcaactc caaaggctgt tggattatcg aaaggacgat
     3661 tgagaaaaat gctagccaac attgctcaat agactaaaaa gcgactgcaa caaaaggcgt
     3721 gtcgcagtct ccttcggaat tagctcaaaa aacggtaaaa tattagaagc tatttttctg
     3781 tttatttctc taatactcag gtcagggcgc tgatttcacg acctcttcct taaacggtgt
     3841 tctcctgcca actcctcggt cacaagtcac aatatccaac aaacatgaga agctgttttt
     3901 tgaaattgct ccttgtgact cagagttgaa cggcaggctc acgacctaat cagaaaaacg
     3961 attaaaattc attcacaaag aaatcgaata aacgctgttc agatgaatct acacgatgag
     4021 aaagttccgg atgccattgg acaccaagga ttttgtgcat attatcttta ctttggactg
     4081 cttcaatgag tccatcgttg gaaaaggcga tggcttttaa caaaggagaa agatctttga
     4141 ctgcctgatg atgataggaa ttcacaaagt atgaatcagg gagaaggtca cttaataaac
     4201 tgtccgaaac gattttgact tcgtgagttg caaactgcgg ggcagtgggc tgttgttcgt
     4261 gttttacgga ccattttgga tataatgaaa gatcttgata aagtgttccc tccaacacga
     4321 cattcaatag ttgaagtcct cggcagactg caaaaatcgg ttttctttgt ttcaatgctt
     4381 ccaagatcaa tgcctgttcg aattggtctc gttgaatatt tgtttccgtc agttttggat
     4441 gagggtcttc catgtaaagt tgcggactaa catcctgacc gcctgcaaga atgagtttgt
     4501 cgattttctc aattagttga ggcgctgaat ctggagacat gactggcaga ataagaggta
     4561 atccttgagc atctaaaact gcatccacaa atccctgcgg tgtatagctt acttgatttc
     4621 cttggaaagt atctgtcgct cgaatcagtt gattcccggc aatgccgatg attggtttca
     4681 tttttattcc ccctaaagta gactaattgt aacaaggatt taaaaaaatt taaactatgt
     4741 aagacttgcc cctatactta tctgttcttg ctgtgttact atctgtaaag agaattttcg
     4801 tgtgaaattc atgacgatag ttgaaaatat tgcttaattt gttataataa aaagtaagca
     4861 gaaataaaaa ggttgggcgt aagcttcaag gatctagttc ttcaaggggt gagaaggaca
     4921 ttttattgaa ggggcctgcc tatactaagt tatctttaga ggtacatact ttcaatgaaa
     4981 aaagaaagca tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt tagaagcttt
     5041 ggaacaaatc cgaaaagcag aagaagaaaa tgagaagcgt gctcgaaccc tacggcagca
     5101 gttgaagacc tatgcagaag aaaaagagca agaattggag ataatccaaa cgaacagtcg
     5161 gcaacagctg aaacagttgg tagaagaaaa agaacaattc gaaaagcaag aattagaaaa
     5221 agaaaaaatg aaattgaaag cagaagcaga caaggtgcaa gcaactttga aagaccaata
     5281 tcaaaagcat cagagtcaag caatcggtgc aatcattgaa agggtgaaag aaacttatgg
     5341 ccgtcactaa gatggaaaaa gtgaccctca tttcagataa aagaaatcag gagaggattt
     5401 tacaagcagt acaaggaatc caaaatgttg aaatccggga tctgtatcaa aatagaacaa
     5461 acaaccagtg ggtagaaaaa tattttccag ataccacgat tattgataaa gaagcaaaat
     5521 taagcgcttt aggcaatcgg ctgaaagaca tccaagaagc tatccaattc atacaacacc
     5581 acggcgatag ccgtcaaaag aaactccatt tgaaacatca agaactttcc ttacaagact
     5641 tggaagcaaa ttattccgaa gaaaatttca tgaataaatt agaagaagtc cttgatttaa
     5701 aagaaaagtg ggaacagcta actgaaaaac gtgatcgttt agaagaagag gaagaatggc
     5761 ttcttcattg gcaacagctg gatattgctc caagtatgta tgcaagtaag acgaccattt
     5821 ttgagatggg gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa ctaagtaagc
     5881 ttgatgacgt ttatatggaa gaaatagatt atacgccaaa acaagtttat ctttcgtatg
     5941 ttgctttaaa aaaatcagtt gctgctatag aagaaattgc tgggaaatac gggttcaata
     6001 aagaaacgta tccttatgaa caaaatccta aagaaaaatt gcaagaaacc aaacagcaat
     6061 tatccattgt tcacgagcag caaaaaaaga taacgacagc gttaggtcag tgcagcggct
     6121 atatccaaga atttgaatgg gtagaagaag tcacacttgc cgttgcagaa cgggaaaaag
     6181 taaaagaacg gtttatccat gccacttatc tgatcgtttt gcaaggttgg gtagatgcag
     6241 aagaaaaaca gaatttgatg catgtgttaa acgaaactgt atcagaagaa gatatatacg
     6301 ttagtttcga aacaccaaac tcagaagaga ttcagcagga agtccctact aaattgaaaa
     6361 accatccgct tatcgaacct tttgaattgc tgactgaaat gtacagtctg ccaaaatatg
     6421 aagaagtaga tccaacgcct tggatgacac cattttatct tgtcttcttc gggatgatgg
     6481 tcgcagatgt tggttacgga ttgctgatgc tcattgggac aatactagct caaaaactat
     6541 ttgttttacc ccgcgggatg aaacgtttcg taaaattctt tgagattctc tcggtaccat
     6601 cgatcatctg gggattgatc tacagttcat ttttcggaca ggcattacca aaagaactat
     6661 ttggtatccg cttgcctttc ccaatcctat ctacgacgga tgacgtcaat acgattttga
     6721 ttttatccgt gattttcggt ctgatacaga tcttggttgg attattcgtg gcagcaaagg
     6781 aaaatatcag gaggaaagat tacttgagtg ctgtcagtga tggttttgcc tggcaaggga
     6841 tcctgattgg gatcgttatt gcattgattg gtgtcatgtt gataaaaaat acagcatttg
     6901 tttatcttgg cgcggggcta tcgatcattg ctgctttatg tatcttaatc gtgccgatta
     6961 tccaatcgcc gtctaaagtc aagggtgctg ccaaaggagc gtataatctt tacggattga
     7021 ctggttatat cggtgattta gtcagttata cacgtttgat ggcactaggt atttcaggcg
     7081 gaagtattgg cgcggcattc aatatgctag ttgctttcat gccgccagcc gctcgattca
     7141 gtgtagggat ccttttgatc atcgctttac acgcactgaa tatgtttttg acactgttaa
     7201 gtgcctatgt ccatggtgca cgattgcagt atgtcgaatt ttttggaaaa ttttatacag
     7261 gaggcggacg tgcctttgac ccgctgaaaa cggcggaaaa atacgtcaac atcaatcata
     7321 agaaaaaaaa caataaataa ttggaggatt tttatacaat gatggattac ttaattagtc
     7381 aaaatggtgg aatggtattt gcagtattag cgatggcaac agcgacgatt ttttcaggga
     7441 ttggttcagc taaaggtgtt ggaatgacag gtgaggcagc agcagcgtta acaacaagtc
     7501 aacccgaaaa attcggacag gctttgattc tgcaattgct tccaggtacg caagggttgt
     7561 atggattcgt tatcgccttt ttgattttta tcaacttagg aaatgatatg tcagttgtac
     7621 aaggattgaa ctttctaggc gcttctctgc cgattgcttt tacaggtttg ttttcaggta
     7681 tcgcccaagg gaaagttgca gcagcaggta tccaaatttt agcgaaaaaa cctgaacatg
     7741 ctacaaaagg aatcattttt gctgcgatgg ttgaaacata tgcgatttta ggtttcgtta
     7801 tttccttcct attagtattg aatgcctaag cataaaggag gaaaagcaga tgaacgccat
     7861 tgaaaaaatc atctctcaga tgaatgaagc agcagagcaa gaaagagcgg cgttagagca
     7921 agaagagcga atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaaa tcgaaacaga
     7981 acatcaaaaa caaaaagaaa aacaaatcga attacttgaa aaaaaatacc gtcagcttag
     8041 gaatcgccaa caagtagaag tacgacaaga aaatttgaat gcgaagcagg aatttttacg
     8101 acgattgttt gcagatgcaa tagctgaaat ggagaattgg gatgaatctg aacaaatcca
     8161 gtttatcaaa aattcattgt attcattacc tttgacaggc aaagtaacat tcattgctgg
     8221 agaaaaatca gcagcttatc tttctcagac attgctggat gaatggaaca atgaacttcc
     8281 ctttatgatg gttttaagtg atgaaactgt tgctgatcaa gcgggttttc tgataaacga
     8341 tcaaggagtt cagtataatt ttcttttcag cagccttgta caagatatcc aaggaacgat
     8401 gagttttgag attgccaatc aattattcga ataaggaggg atcagtgtat ggaataccac
     8461 gaactaaatc ccttgattcg cggaagagag cttgaacttt tgtccaatga gacatttgaa
     8521 cggatgattc aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc cacgatttac
     8581 agtccatata tttatgacgg atatgaatat gacttcgaac agaatctttt aaaggagcag
     8641 agccgtctat ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt ttggatctat
     8701 acgatgcgct atactttcca taatctgaag gttttgacaa aagccgagat gacaaatcaa
     8761 aatctggacc atttgtatat cgacgatgga ttttattcat tagaaacctt gaaggatgcc
     8821 attcatacgc aagtttcaaa tgaactccct gaaagcatta tggacagtat acgagaagta
     8881 cgtgattatt ttgaagagtc taccattctt cagggaattg acgtgattta tgatcgacag
     8941 tttttgacag agcagcgacg gcttgccgat aagctaggat atcaagaatt gctgacagaa
     9001 gtcattgctt ttattgactt gaccaatatc acaacgatgg ctcgaggaat attgcagaaa
     9061 agaacatatg ggttcatgac gacggtactt tccagctcgg gaagtatcga aaaagaagta
     9121 tttttgaact ttgtagataa agatatggaa gattacacga attttctatt agagacagat
     9181 tatgctgatt ttctgcgccc tgtcatccgt gagggaaatc ttgatctcgt aaagcttgaa
     9241 cagttgaaag atgattatct aagttcattg tatcaagaag cacaaaccca ggctttcgga
     9301 ccactgccac tactagcctt tttgaacgca aaagaggtag aaagcaaaaa ccttcgttta
     9361 ctggttatcg gcaaaagaag ccatttcgaa ccagaaacaa tcagaaagcg ggtgagacaa
     9421 gtctatgacg cataagatcg gtgtagttgg cgacaaagat tctgttttgc cattcaaatt
     9481 gtttggcttt gatgttcgtt atggtacaac caagcaaacc gttcgtcagg caattgaaga
     9541 aatggcaaag aatgagtatg gcgtgattta tgtcacagag caatgtgcag cattgagccc
     9601 agaaacgatc gaacgttaca aggaacaaat gatacctgct atcgtattga ttcccaatca
     9661 tcaaggatca ttagggatag ggctggaaga aattcaaaaa aacgtagaaa aagcagtagg
     9721 tcaaaatatc ttatagtatg aaaaggagat agaaattttg caaattggaa aaatcataaa
     9781 agtctccgga cctcttgtaa tggctgagaa catgtctgat gccagtatcc aagacatgtg
     9841 tctagtggga gatctaggag ttatcggaga aattatcgag atgcgtggcg atgtggcgtc
     9901 tattcaagta tatgaagaga cttctggaat tggtccaggc gaaccggttc gctctactgg
     9961 tgaagcttta tctgttgaat taggaccagg gatcatttca caaatgtttg acggaatcca
    10021 acggccatta gataccttta tggaaatcac acaaagtaat ttcttagggc gcggagttca
    10081 attgcccgca ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag aagccggaga
    10141 aacggtgagt gctggagatg ttatcgggat cgtagaagaa acaaaagtta taaaacataa
    10201 aatcatggtg ccaaatggga taaaaggaac gatcaaaaca atcgaaaaag gttcattcac
    10261 tattgatgaa acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt tgacgatgct
    10321 tcaaaaatgg ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc cggatgcacc
    10381 catgatcact ggacagcgag tgattgatac attcttccca gtgacaaaag gaggagcagc
    10441 agctgtacct ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa ttgcaaaatg
    10501 ggccgatgtg gacatggttg tctatgtggg atgtggcgag cgtggaaatg agatgacgga
    10561 tgttttaaat gaattccctg aattgattga ccctaataca ggagaatcgt tgatggaacg
    10621 tacggtattg attgctaata catcaaacat gccagtagca gctcgagaag cttctatcta
    10681 tacgggaatc acaattgcgg aatatttccg tgacatgggt tatgacgttg ctatcatggc
    10741 agactccact tcacgatggg cagaagcact tcgtgagatg agcggacgtt tggaagaaat
    10801 gcctggtgat gaaggttatc cagcttatct tggttcacgg cttgcggaat actatgaacg
    10861 atctggaaga gtcattgcat taggatcaga aggtcgcgaa ggaagtataa ccgcaatcag
    10921 tgctgtatcc ccgtctggag gggatatttc ggaaccagtc acacaaaata cattgcgggt
    10981 cgtaaaagta ttctggggtc ttgattccag tttagcccaa aaaagacatt ttccttcgat
    11041 cgattggata caaagctatt ctctttattc aacagaagta gggaagtata tggatcatat
    11101 ccttcaagct gactgggcat ccatggtcac cgagggcatg cgtattttgc aggaagaaca
    11161 gcagctgaat gaaatcgtgc gtttggtagg gattgattcc ctttcagata atgaccgtct
    11221 gacattagaa gtggcaaaat ccattcgtga agattatttg cagcaaaatg cttttgatga
    11281 tgtcgataca ttcacttcaa gagagaaaca attcaaaatg ttgaagatga tcttgacctt
    11341 tggtgaagaa ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga tcatggacgg
    11401 taccgtatcg attcgtgaaa ggatcggacg aagcaaatat atcccggaaa cagaattaga
    11461 aaaactagat caaattattg gtgaaatcaa gcaaacgatg caaacgatcg tttcagaagg
    11521 aggcatgagc aatgattaaa gaatatcgta cgatcggtga agtcgtcggt ccattgatgg
    11581 cggttgaaaa agttgctggt gtcaaatatg aagaattaat cgaagtccgt atgcaaaatg
    11641 gcgagattcg tcgtggacaa gtattggaag tccaagaaga taaagcaatg gtccaaatat
    11701 tcgaaggaac aagtggtatc aatttacgtg actcttctgt acgattttta ggccatcctt
    11761 tagaactagg tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg ggacgaccga
    11821 aagataacgg accagagatc ctgcctgaaa agcatttgga tattaacgga gaagtaatca
    11881 atccagtagc tcgggattat ccagatgagt ttatccaaac aggtatctct gcaatcgatc
    11941 atttgaatac attagttcgt gggcaaaaat taccagtatt ttctggctct ggtcttcctc
    12001 ataaagaatt agctgctcag atcgcgcgac aagcaacggt actcgattca tcagatgatt
    12061 tcgccgtagt ctttgctgct attggaatca cttttgaaga agctgaattt ttcatggaag
    12121 attttcgtca aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta gcaaacgacc
    12181 ctgcaatcga acgtatcgct actcctcgta tggctttgac tgctgcagaa tatcttgctt
    12241 atgaaaaggg aatgcatgtt ttggttatca tgacagacat gaccaactat gcagaggcat
    12301 tgcgagagat ttctgcagca cggcgagaag ttccaggaag aagaggatat cctgggtatc
    12361 tttacacgaa cttagctact ttattcgaac gagcaggaag aatccgcgga ctaaaaggat
    12421 cggttactca aattccgatt ctaacaatgc cagaagacga taaaactcat ccaatccctg
    12481 acttgactgg ttatattaca gaaggacaga tcatcttgac tagagagctt tacaagagtg
    12541 ggattcagcc accaattgat gtactcccgt ctctttcgcg gttaaaagat aagggaactg
    12601 gacccggaaa gacaagagaa gaccatgcag ctacgatgaa tcagcttttt gcagcctatg
    12661 cacaaggaaa acaggcaaaa gaattagccg tagttttagg tgaatctgct ttatctgatg
    12721 tagacaagat ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc aatcaaggat
    12781 tctacacgaa ccgttcgatt actgaaacac ttgatctagg gtgggaactg ctatcgatgt
    12841 tgccaagaac cgaattaaaa cgaatcaaag acgacatgct ggacaaatat ctgccagaag
    12901 ggaagtgagt tctgatggca cgattgaatg tgaatccgac ccgcatggaa ttgactcgat
    12961 tgaaaaaaca gttgacaact gccacaagag ggcataaatt attaaaagat aagcaagatg
    13021 aactgatgcg tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt caagaaatgg
    13081 aagagtctat gacagaagca atgtctgatt ttgtattggc aaatgcttcg atcacggaac
    13141 ctttcatcga agaattattt gttttgccgg cggaaaacgt tgatttgtct atcgtagaca
    13201 aaaatatcat gagtgtaaaa gtacctgtga tgaattttga atatgatgaa cagctgacag
    13261 acacaccttt ggaatacggg tatttgaatt ccagtgccga actggatcgg tcaatcgatc
    13321 ggtttactac cctgcttcca aaacttttgg aactgacaga aatcgaaaaa acttgtcaac
    13381 tgatggcgag tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa tatatgacga
    13441 tccctcaatt agaagaaacg atctattata ttcgtatgaa gctggaagaa aacgagcgcg
    13501 cagaagtaac aaggttgatc aaagtaaaaa atatgggtac cactgactaa gtggtatctt
    13561 tttttaaaag aggaaaacga tgattaaaca aagcaaacaa ataaagaaac gtttgccggc
    13621 tgttcagctt ctcgcattag gattttttct cctgatttta gttggcggcg gcctacttac
    13681 cctaccattt tttagtaata gtgggcatgg gacaaatttt atcgatgcgc ttttcactgc
    13741 aacttctgct gtctgtgtaa cgggattgac aacattgaat acagcagagc attggaacag
    13801 tgctggtcaa tttttgatca tgcttttgat tgagatcggc ggtcttggtt ttatgatggt
    13861 tccgattcta tgctttgcat tgataaagaa gaaagtcagt ctcagcacac gtattatatt
    13921 aaaagaagcg ctgaatctag aagaagtatc aggtgtgatg aagctcatgc tttacatcct
    13981 aaaattggcc tttgttatcc aattgattgg tacggttgct ttatcttttg tatttgtgcc
    14041 tgaatttggc tggttgaaag gtatctggta cagtttgttc catgcaatct ctagtttttg
    14101 taatgcaggg tttgatttgt taggagacag tttagccaat caccagcaaa atgtttattt
    14161 gatcatggtg gtatctgctt tgatcatttc tggtgggtta ggcttcatcg tatggcgtga
    14221 tttactggaa tatcatcaga aaaagaagat gacgattcat tcaaaaatcg cattaaccgt
    14281 tacaggtacc cttttattag gcggattcgt tgtgttcttt ttcacagagc gcaatggggc
    14341 aacgctagtg gaagggaata tgtttcaacg attggcaaac acctttttta tgagtgtgac
    14401 acctagaacg gctggatatt attcagttga ttacctccat atgagccatg caggtctgat
    14461 tttaacgatg tttttgatgt acatcggcgg aacatctggg tcgacggccg gcggattgaa
    14521 aacaactacg ttaggtgtgt tgctgatcca gatgcgttcg atgttgaaag gacgtacgag
    14581 agcggaagca tttggccgta ccatccgaca aggcgcagtg ttgcgggcac ttactttatt
    14641 ttttgttacg ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga ctgaaacgat
    14701 acctaaaact tctggcatcg aatatattgc ttttgaggtc ttctctgcgt ttggtacagt
    14761 aggtcttacg atgggattga cgccagactt gactgcaata gggaaattga tcattatagc
    14821 gcttatgtac attggacgag ttggtgtatt gactgtggtc ttctctctga tgacaaaagc
    14881 acaaagaaca caatcaaaat acaaatatcc agaagaaagt gtcatgattg gataatttca
    14941 tcttctgaca gaaacctagt gatttagtaa aataagaaaa taaaataatt tttcttgctt
    15001 cttggatact ttaagtgcaa aatgttcgtt tattttttga ggcactaccc gttgttgaac
    15061 ctcgggtagt gcctcttttt tattgaaagg aatcaataag atttgattcc tttatttatc
    15121 aataggttat aattcatatt tttttatttt ttcttttacg atacttagtc cagaagctaa
    15181 aatttcaagg ttattagagg atagggaaat tcgaacgaat gcatctggtt gcgcacgtgt
    15241 agtaaagcgt ctggaatgat aaagtcggat accgttagca agaaagtcat tctcaattat
    15301 cttagggtca atctcaggat caactggaag aattcgatag taagggacac gattcgttgg
    15361 tttttctact ttgaaaagag aatcaaaaag ctgattggct ctttttgtaa gacttaattt
    15421 agtttctaag atcctatttg cttcatttga atggaggatt tgagtgatga cttccacatc
    15481 taatccagaa gttttcacat ttatattaaa aattgcttgt tctatcttgc tttggacatg
    15541 tactggaact acaagataag caactcgcaa tccagaacaa ataaattttg tcattccagc
    15601 taagtagatt gtttggttag gtaataaatt ttggaaagga ggaatgatcg tatgttgata
    15661 gtaagttgtt aaaaatgaat ggatatcatc ttcaataacc catagacctt ctttttcaat
    15721 cacttcggct aattgctctc gtcttttgtg agagatttga aaccctatag gattattaca
    15781 agaaggcata agaaagacac catgaatctt cttttttcta cattcctgaa tcaatatatc
    15841 aggtcgcatt ccttgctcgt caaaatcaat tggaacgatc tctaaatgat ataactgagc
    15901 caattcaatg aaattagcgt atgtatatcg gtctgttgct attctatcac ctggagagaa
    15961 aagtgcagaa agtgtgatag ccaatccatt ttgaacaccg gaaacaatag atagagattc
    16021 taaggacgga atgactcctt gagtttttaa ccaatttgct gcagtagtca attgaaaatt
    16081 tgtgcctttg ggatcttggt aattaagcaa accaacaaga tttgggttat tactagttga
    16141 ttgaataaac gggacaacga atttattaca ttcttcaaaa gaactaacca gaccaaaatc
    16201 aatgatatgt tcggttactt cattggtaga gatggtgatg gactcaaaga cattgggaga
    16261 aataaatgtg ccacttccaa tattcgtata cagcaaacct ttctcaattc catatttata
    16321 agccttgcca acggtagtaa aatttaaatc taaataatca gctagctctc tttgggaggg
    16381 taactgagtg tttttctgta atttattctt taaaatatct tgttctaatt gttgaatcaa
    16441 cgatcgataa taaggacgct tcaatttttg tttgtctggt cgccaagtta acgggtaatt
    16501 gtcataagaa ttaataggca ttttctccac tccttaaaat tgtataccat acaattttac
    16561 cattgattgt attgatttta gaagttaatc ttttgtgagg agggaattgt ctatgaaaat
    16621 tattaatttt gaggaagtag tagcttcttt tagttttgaa gaagcgattc aagtaatgaa
    16681 aaaatgtttt tttgattatg aaagtggtaa aatcagccaa aatcctcgat tggtggaaat
    16741 tttacctgac ggaaaaggca aaaatatgtt tgcgatgatg cctgcttatt tgggggagaa
    16801 cagatatttt ggcacaaaag tcattaccgc ttttccggat aatcatcagt atcatttacc
    16861 ttctcattta ggagagattt tcttgtttga tgctcaaaac ggactaccca aagcattgat
    16921 aaacgcaaat gcagtcacct ggattcgaac agctgctgta tctgcgctgg caacagatta
    16981 cttagcaaat ccgcatgcag aaacacttgc tttaattgga gctggtcaac aagcattgtc
    17041 gcatttgact gcgatttcta ccattcgtcc catcactaca gtgtttgttt acgatatttt
    17101 agaagaaaga cgaaatactt ttatcgctaa agctaaaaaa cagtatccta aaataacgtt
    17161 tataaatgca atatcagtag aagatgcagt acgtccagcg gaaatcattt gtacgttgac
    17221 ctctagtaaa gaggcatttc ttcaaaaaga gtgggtaagg gaagacgcac atatcaatgc
    17281 tgtgggcaca tttactcctg atacaagaga gataagtaca tcgttgatgg aaacttctca
    17341 aatctatgta gatgattact cagctgcctt aacagaaagc ggggacctgc ttatagccat
    17401 ggcagaagga gatttagagg aatcttgtat tgttggtaca ttaggcgaat tagtttctga
    17461 gaaaaaaaca ttttataaag gagaatcgag gccgacgatt ttcgacgcag ttggtctagc
    17521 tattgaagat ctttgttgtg cagaatatat ttataacaaa ttagagaaag gggctttaaa
    17581 atgaacttag attattacgt agttgatgca tttgcagatg aggtgttcaa aggaaatcca
    17641 gcagcggttt atgtgctaga agaatggctg ccagaaggta cgatgcaaaa aattgccatt
    17701 gaaaacaatt tatctgaaac agcatttaca gtgaagaaaa accaagaatt tgagcttaga
    17761 tggtttacgc cagatcgaga aatcgatctt tgtggtcacg cgacattagc aacagcattt
    17821 gttttattta actattacaa aatacctgat gaaacgatta aattttctac tcaaagcggt
    17881 aatctatttg tgacaagaat taaagattat tattacatgg attttcctag tattatgccg
    17941 aaaaaagtgc cgatattgtc tgaatatgaa gaagcgattg gggctaagat taaagaagcc
    18001 tacttagcac gtgatttatt tttcgtatta gaagatgaaa aaacggtggc taaattgcag
    18061 ccagatttta cagctatcaa aaattttgaa ttaggaatag gagtcatcgt gacagcagaa
    18121 agtgtgcaaa aagactttgt ttctagaacc ttctttccaa aattaacaat caatgaagat
    18181 ccagtatgtg gatcagcaca ttctaatctt attccttatt gggcagaaag aatgaataaa
    18241 acaaaattgt cagcttatca aatgtcatca aggggcgggg atctagaatg tgaattaaaa
    18301 gaagatcgtg tgataatagg aggaaaagca gttttgtttt ctaaaggtac ctcgtttatt
    18361 tgatttcttt caaatgaatt aaaaataaat tggtggagag tggcaacctt atagctacaa
    18421 tggttgttaa aaaaagacgc aaactttttc aatttaatga aaagtttgcg tctcatttta
    18481 attattggtg tttgtttttt attcaatcaa gagtaaaatg ccccatacta actatcctcg
    18541 gatgacttcg attttataac catcagggtc aatgacaaag tagtaagaag gcgcagtgcc
    18601 aggtaatcct tttaagtcag taatctcaag accggcagct ttatgttgct cgtgtaattt
    18661 ttctagatca tcagctgcta tagcgatatg accatagccg tctcctagat catacgcacc
    18721 atgatcatag ttgtaagtca actctaattc atagtcatcc ccaggaagtg tcaaataaac
    18781 aagtgtaaat ttgttttctg gaaaatcacg gcggcggctt tcttcaaaac caaatgcctt
    18841 ttgataaaat tcgattgatg cttcaagatc tttcactcga acacatgtgt gagccatttt
    18901 catagattca tctctccttt tccttttact caatcataac aaaaagaagt aaggaaatag
    18961 ttgatttgtg cttgttcatg agaaatttct catgtaaaat ggatagaaaa taaaactgtt
    19021 aaggtggaaa ctattcatgg ataaaccaat ttttggtgaa aaaaagcaat ttgctgatta
    19081 tcaaacccgt tatgcagcat atattgtcat agaaagagat cagcagattg cagtaatcga
    19141 ggctccaaat ggtgcttttt ttcttcctgg cggggaaatt gaaggagaag agacgaaaga
    19201 agaagcgatt gtccgtgagc tgatagaaga aatgggaatt gtggcagaaa tttcttctta
    19261 tctgggagaa gctgatgagt atttttactc gaattatcgt gcaacttatt attataatcc
    19321 aggatatttc tttgttgctg attcttggaa gcgtatagga gaaccaactg aaaagaccaa
    19381 taaaatttgg tgggtgagcc ctcaagaagc gcttgtgaaa ttgaaacgtg gtagccaccg
    19441 atgggcagtc gagagatgga tcgaaaaaaa taaaaactga aagaaagcga gtagatcagc
    19501 tttctctcag tttttttatt tattacagta catatttttt gattgcttca gctacaccgt
    19561 gctcgtcgtt tgatgaagtg atgacatttg ctgcttcttt gacaagagaa acggcatttc
    19621 ccattgctac tcctagacca gcatattcaa tcatggataa gtcgttctcg ttatctccga
    19681 tagccatgat ttcatcttga tcaataccaa ggtgctgtgc gagattcgca actgccgcac
    19741 ctttacttgc ttctttattt agtacttcga agtaataggg cgcacttttg accgttgtgt
    19801 atttatcacg gaaagtttgc ggtaaacgcg caattgctgc atctaaaatt tctggttcgt
    19861 cgatcatcat cattttgacg atgttcatgt ctggtgtcat ttcttcaggt gttcgatatt
    19921 ttagcggcat cttgactaaa cttgcttcgt ggatcgtata agcgctgata tctcgattag
    19981 gcgtgtagat cgctttgtca tcgatcgaat ggagatgact gccgacacgg cgtgccatca
    20041 cttcgatctc taggaaatct tcatgtgtca gtccgtgacg ggcgatgatt ttacctgatt
    20101 tcgtttgttg tacgagtgcc ccgttataag taataacgta atcattatct tgaaaaaggt
    20161 tcaactcgtt taactgttcc tggacacctg gtaagggacg gccagtgcat aaaacgatcg
    20221 aaacaccttt tgcaacagca gcggcaatcg cttcttttac ttcttttgtt acttctcttt
    20281 tctcatttaa taaagtacca tcgatgtcga tagcgactag tttgatagac ataatttcct
    20341 ccaatatatt ttaatttatt tcaatcaatg cgccattacg gatatggctg gcaaattctt
    20401 gataagtttc gtcaaataaa tcataatgat cacgcaagga cggatcaacc atttcttttg
    20461 gaaagaaaaa tcgctcatct ccttgttctt gtcccgctaa tgcagcaaca agctgactaa
    20521 cctgtgataa ttctatcaaa gttccgtcat ttcgcaaaat ctcgatttgt gtgcgatgtc
    20581 tgccttgttc tgggcgataa aaatcataag gtagatcgta gctagaattc acagcagtgt
    20641 aataaacagg attatatccg accttttcta ctaacaaagt gagttcttgg actaattttg
    20701 aatcccggtt gccagaaaaa gtagcagatt tcagtggttt acgattcaaa aaacgtttag
    20761 ctaaatcatt taaaatagga tcagcagatt cagaccattg agtaaagtaa gtgcccaaga
    20821 ccccatcatc aagtttgagg tattcttcta aagtaaagct tccgtcaaga aatggcagaa
    20881 gtaattgaga atggagttca aaactgctgg catcttcttg atacaactcg tgtgcgcgat
    20941 gaagcagatg ctccaaaatt acttccatac cacgggaaac aggatggaag tacacttgca
    21001 catacatttg atatcggcta acgatataat cttccactgc atgcatgcca ctcatggaaa
    21061 aagcgatccc gccttcataa ggacgaatca cacgtaatat tcgggtaaga tcaaatgttc
    21121 catattctgt tccagtgaaa taagcatccc gaagcaaata gtccattcga tctgcatcga
    21181 tctgactaga aatcatttgg acaacttgtg ggttggggta agttttttgg atcacactag
    21241 ctaccttttc aggaaaacct tcttctactc ggttaagaat ctgatacact tctgtttctg
    21301 gagaagtgat gatctctaca gtgatggctt catgattcgt atggaagata tgttcaaatg
    21361 tatgagagta aggaccatga ccgacatcat gcaacagtgc cgcacataaa gtcaccagcc
    21421 gttcctgatc atcccagcct ccgttgccta ttttttcttt tgaaaaattt cgagaaaaga
    21481 tatcgcagat tcgtcttgaa atctcataaa cgcctaatga atgagtaaaa cggctatgtt
    21541 ctgctccgtg aaaagtgaaa gaagaagtgc ctaattgttt aatgcgtcgt agacgttgga
    21601 cttctttcga attgatcaaa tctaagatta cttgatgctg gacatggata tagttatgga
    21661 ctggatcacg aaaaactttt tccataggca gcatttgata tttataaggg gttgtcataa
    21721 tgtagctcct ttatgttatt acgttgttcc attctctgga tttggttgtt ccattgctcc
    21781 agctggattt ctttttttag ccagtcggta gaatcctgta aatcacttgc agaatacagc
    21841 ttttcaaaag cttttgcaaa tgcttctttg acttctttta caaaaagggg ttggtcaagc
    21901 agttcttcaa gtgttgccat tgtagcagga tccactgcag gatagccatt tgtaccaaag
    21961 tcttctttta aagcagattg ataaaatcgc ttcatgactt gcccgcgttt ttcctgatta
    22021 ccgttgacac ttaagtacat cataacagaa atcccatttt tgatccgacg ctgagcgata
    22081 ccggcaaatt ttttgccatg gatgcttaaa tcataagttc caggacaata agaatgggtg
    22141 atttcgaatg cttgtatcgg tgcctttgga aaagcgtatt tagtcaatag gagcatttgc
    22201 tcgtaagcgt catctatggt tatttttttt gtgtcacttt gggggaaaat caaagaaata
    22261 ttcaaaattc cttcatctga tacgatccct aatccacctg aatttctgat cactggagcg
    22321 taacctgcat ctttcagtgt ttggatcccg tcacttagaa aaggagttcg aacatccttc
    22381 atacctaaga tgactgtgca gtcatattgc cagaaatgta aaaagatttg ctggtgttct
    22441 ttagaatatt ccgttaatat atctgttaat gcaaaagggg aaaagtcgtt tttttgataa
    22501 atgtgctgat ctagtcgaat cgcagggttc attcaaccgg ctcctttgta taaattcata
    22561 ttttctacaa tattatactc taaaatcaaa gagttgtttt ggaaaaaagg ttttctgtaa
    22621 aaatggacaa gttggttgac aaaacaaaag aaactaggaa caatgtagct atgagaagta
    22681 aaggagaatg aataatggaa ttatcacaag gaacaccgat ttcaatcaaa ttgcgtacaa
    22741 aagtaaaaca gcaaggggaa gtgcaagatt tttattttga tttgaaaggg caaatggtga
    22801 caattggcga tacgctgtac atacgttata aagagattca ggaggagacg ggcgatgaga
    22861 ttcctgtaac gatcaaatta gttccagatg gtcatgtaca gctgatccgt gcaggagaaa
    22921 tgcggatgcg tttgaaattt ggctataaag aacggttaga aacatcctat cgaacaccat
    22981 acggtatgat ccagattgct acttttacga aagaactgca tgttagtttg aaagaccgtc
    23041 ccacaggagg gaaggttcga atcgattatg atctgttcat gggaccggaa aaaatcggcg
    23101 aatattattt gaccttggat tttacagcgt aaatataaaa gaaaagtttg attttttgtg
    23161 tctgattttg tatgataagg agtagactgt gaaaggacgt gtcctgattg gaaattagtg
    23221 tatttgaagg tgcaaacaaa agcgaattat caatgatcga agtagcacac gcgatcttag
    23281 aacaacgtgg cgatgtcatg gatttttctg atttagctaa ccagatccaa aactatcttg
    23341 ggaaatcaga cagtgaaatt cgtgattcac tcgcacaatt ttatactgac ttgaatattg
    23401 acggcagctt tatctcatta ggggataatc gttggggctt gcgttcatgg tatgctatcg
    23461 attcgatcga tgaagaagta aaccatggaa tggacgaaga ggatgaagat actccacgtc
    23521 gccgtaaacg caagaaagtc aacgccttta tcaatgatga tgaagacgcg atcgattaca
    23581 atgacgatga cccagaagat acagatctga cagaagaaga tgacgatgat ctgtttgatg
    23641 acgatgatga tgaagatgaa gaaatcgctg cttataattc tgacttgcaa gaaatcggag
    23701 cagatgaccc gacagatgat gaagaagact tgccaggaaa tatcgaagaa gatttaagta
    23761 tcatagacga agacgatgat gatgaagatt atgaagagga agaattttct gactctgaaa
    23821 aagaataaac attattttct tacgagaggt tgtgacagag acgatcagcc tatcagaata
    23881 aggaggtgtg acacgatttt tgtcacacct tcttttttgt atataaaaat cccaagtaaa
    23941 tacataaaaa ataaaaaaat tgaaactttt agcgcacctt gtacgtcata ttaagtgagg
    24001 cagaaaggag tggagaatga tttttactgg atataagaaa aaacggcaga tcgaaaaata
    24061 ccgtcacctg ctgaatggac tcatcgaaac tgattatcaa aaaatgttcc gtatcacgat
    24121 gaattacgta catgataaag aagaggcctt agatgtgatg caagatagtt ttcataaagc
    24181 cctgaactat tttgaaaaag aaaaagagat cgagcatttt agtgcatggt tttatcggat
    24241 attgatccgc acggcattag atagttggcg cagacaaaaa aggacgcctg tggacttatt
    24301 tgaaattcaa gatatccccc aacagttacc tttagaaact tccgttgcag agctccatat
    24361 gattttagat aaaatagaca gtccggaaaa agagattctg atcctctact tttttgaagg
    24421 atttcgtttg aaagaaattg cgactatctt ggatatgaat gaaaacacag ttaaaacgaa
    24481 aatgtaccgc tcattgaatg ctttacgtca aatcctagaa tgaaggagag aagagtgcca
    24541 tgtattccca aaaagaaaat ttaaaaaaac taaagaaaac ttataaagaa caagcaattc
    24601 ccaaagggct taaagaggag atccgccagc gttttattat cgaagagcaa tcatttataa
    24661 aagcaaagcg gagaaagaga cgtctccaag ggttcggagt atctttggct atcgtaatga
    24721 ttacaacaag tagccttctt tttaatagtc aagttcgaag ctttgcagaa gatttaccca
    24781 tcttaggatc agtcattgaa ttgatcttag gcgaacgctt tactgatcga tctgaaaaaa
    24841 tcgatattca ggtaccgaag atcaatacac aaaatgagaa agaaaataaa acaattcatg
    24901 gattaaatca aaaatacttt cgcgaaggac aagcagattt tgaaaaagct gaaaaagaat
    24961 atggaaattt tgaaacggat cattaccaag ttttgggaga ttatcagaaa atcgtagacg
    25021 ataaccggtt tctcgtgatt gaaaggcaga tcacccaaac agcagcagat agccatgttg
    25081 aaaaaagata cgacacaatc gacaagaaaa acagtgtcca gctctcactt ccacttttat
    25141 tcaaagatga cacctatcta tctgttttga caaaagaagt caaacggcag atggccgaac
    25201 aagtcaagga agatccatct aagtactatt ggactgagca agatattcaa gaagggacga
    25261 tagaaaaacc aacacttgtt acgcctacac gaagttttta tttgaacaaa gagcatcaat
    25321 tagttttgac tttttctcaa tacgaaattg ctcctggtta tatgggtaca ccagaatttg
    25381 tcattcctaa gtccgtgaca aaaacgattt tggcatcgga agattatttg gatcattaga
    25441 cgagaagtag ttgcgactag cagtttcaat tttcaagtaa gtacaagtaa ataataagaa
    25501 gagaaaaaga gaaagaagag atatagtgaa aagaaattat ttagttatca gtattattgc
    25561 tttatccgtg cttttgtttt tcgaaattac ttcggtggcc tccttgatca ccgaagtaaa
    25621 aggacaaaca cactcttcgg agcattcgtc acaatctttc aaacaatcag aacaatcaac
    25681 cattaaaagc caatcaactg aaacagctaa cgtcaaagaa gccattcaag aatcagcgga
    25741 aaaggtacaa agtcagtcat cacaatcaac acaagacagt acaaaaacga gccgagatcc
    25801 ggctgtcaaa agcgtcgcaa tcagctttga tgatggacca ggagcgacca caacaccgca
    25861 gcttctgcga atcttaaaag aaaaaaacgt gcatgccact tttttcgtct taggagaaaa
    25921 tacggcgcag catcctgaaa tcgtgaagca gacagctgaa gcagggcacg agattggcaa
    25981 tcacacatac gatcatcaga atctggccac tctttctgcc cagtcgatga cggaggaagt
    26041 gacgaaagca gacactgaga tcaaaaaagt cactggcaaa acccccactt ttgttcgtcc
    26101 gccctatggt tctgtcacga atgtaggagc aacgatcatc cagcgaccga tcatcgaatg
    26161 gtctgttgat tcagaagatt ggaagacaag aaatcctgat ttgattttgc agaagatcca
    26221 agcaacagta tacgatgggg caattatttt attccatgat atctatcctg aaacgattcg
    26281 tgccgttcct caagtgatcg attatctgaa agagcaaggg tatcgcatca cgacagttgg
    26341 ggatttatta gggcatccaa cggctgtcga gaactattat ggcagaaacg atcatcggcc
    26401 agttcagtaa gtgagacaag tatctttaat aaataacaca ttttaataag tagttgaaca
    26461 cataactacc ttacttgcaa aggcttaaca atatctatac aggtacaaca aaaaactgtc
    26521 caccaatttt ctttggcgga cagttttcac ttaaaaataa agaagcaact attcgtaaat
    26581 tatgctaaaa tagcagtaga acgcgtcctg agggaatcga acccccgtct caagaaccgg
    26641 aatcttacgt gatatccact acactaagga cgcaagcact gggataattg taactaaaca
    26701 gataaaaaat tacaagggga ttggacaaaa aaatgaaatt tgagcaaaat ttttcacaaa
    26761 aacagcagca gacacagaaa ctagccatga cgcaacagtt gcaacagtcc atccaagttt
    26821 tacaatataa cagtgaggag ctgttggcat tcgttgagaa tcaagcaatg gaaaatccat
    26881 tggtggaagt tgttgagccg gaatggcagc cagattacat aaaagcttct tcatcttctt
    26941 atgaaggaga agagacaaat tacctaaatc agattcctga tacaaaaggg tctttatttg
    27001 attcattgat cgaacaagtc catttgaatt accgagatac ttttttgagg aaaatcgttc
    27061 tttatcttgt agaatacatc gatttgaacg gatttttgac gattagttta gaagaagccg
    27121 gaaaagagat tggagctacg cctatccaaa tgctcgatgc gttgactctg attcagcaat
    27181 tagaaccagc tggagtaggt gcacgtagtt tgcaagaatg tctcatgctt caaacagaac
    27241 gagatgatta tgcgccggaa ttagcttata tcgtgttgga agaatgtttt gaggaactgg
    27301 tggagcgaaa atggaaagaa atcgcacaac gtttttctgt tgatctacat gctgttcagc
    27361 agatattcga ctatttacaa acgttgagtc caagccctgg ccggattttc gaccgtagca
    27421 gcgaattgta cattattcct gatgtccggg tattagtgga cgacgataaa aatgtacaag
    27481 ttatctcaaa ccgaaaaaat cagccaaata tacgctttca agagagttac ttcaaacaaa
    27541 tgtcacaaca agcagataaa gaaactgaaa attatttaaa agaacggaaa caggaattcg
    27601 aatggttgaa gaaaacgatc ctacaacgtg gggatacaat tttgcgggtt gctcaagtca
    27661 ttgtttcccg gcaaaaagct ttctttatcg acaaggaacg tccaatcaag cctcttacgt
    27721 taaaggaagt tgcaactgag atagatgtgc atgaatccac ggtcagtcgt gcagtcaacg
    27781 ggaaatattt ggaaacagac ttcggtgtat ttgaattaaa aaaatttttc actacacgaa
    27841 ttcctacgaa tagtacagaa cagacagaag acctttcagc agatacagcg aagaaaaagc
    27901 tgcaggaatt agtcgatcaa gaagacaaaa acaagccgtt gtctgaccag aaattggttg
    27961 aattgctgaa aaaagatgag atagcgatct caagacgaac agtagcaaaa tatcgtgatt
    28021 tattaggaat cccgagttca tcaaaaagaa aacgttatga taactaatca gtcaaaagac
    28081 tagaaaaagt atttcacttt ttctagtctt tttagttgaa aaacgagagc aaaactgtta
    28141 gacttacagt cgtgaggttg agatttccct ttattgatgt gaaattcgaa tagttaattt
    28201 ctttttttat cgtatatggg tcgttttaag tctacattgg acgtaaagtg tccaactaag
    28261 gagatcgcta tgctggatga aataaaaatg attgaatctg ttgcaccaga tattatcgaa
    28321 gtgttgcaag aacgctataa aattttacga aatatctatt ggatgcagcc gattggacgt
    28381 cgaagtttat cggaaagtat ggggatcaca gaacgagtat tgcgaacaga aacagatctt
    28441 ttgaaaaatc tgaatttgat cgacacctca aaaagcggta tgacgctgac aactaagggc
    28501 gaagaagttt atcaaagcct tgaaaacttc atggatcagc ttttaggtac gcatcaaacc
    28561 gaacaacagt tggcagaata ttttggtaac caacgctgta tcgttgtttc aggaaacagc
    28621 gaagagcaga ccaaagtggc tgatgcattc ggcgaagcgc tgagcgaagc cctagaccga
    28681 ctacttccag aaggcgagaa tatcatcgct gtgatgggag gaactactat ggctgtggta
    28741 gccgaacaat tgtctaatct ggaaaataag aaacggcata acctgtttgt ccctgcaaga
    28801 ggcgggattg gtgaagcgat aactgtacag gcaaattctg tcagtgcaag aatggctgct
    28861 aaagcaaacg gtaaccaccg tgcactttat gttccggaac aattaagtct ggcaacttac
    28921 aattctttac tcaatgagcc atctatccaa gaagtcttaa acttgattag tgaagcgaat
    28981 tgtgttattc atagtattgg gcgtgcatta cacatggcag cacgtcgtaa gatgactgaa
    29041 aaagaattag tcatgctgaa acaagcaaat gctgttgcgg aatcttttgg ctactttttc
    29101 aatgaaaaag gtgaagtcgt ttataaggtc cctagaatcg gcatagaatt gcaagatttg
    29161 gaaaaagtcc cgatcatcat ggcaatcgcg ggcggaaaat caaaagccaa agcaattcga
    29221 gcttatatga aaaatgcacc aaaacaaacg tggctcatca ctgatgaagc tgctgcaaat
    29281 gagattttaa aaggggtaac cctttaaaat aaatattttt ttgattttca taaggaggaa
    29341 atcttaaatg acagttaaag taggtattaa tggttttgga cgtattggac gtctagcatt
    29401 ccgtcgtatc caagatgtag atggaatcga agtagtagca atcaacgact tgacagatgc
    29461 taaaatgttg gcacacttgt taaaatacga cacaactcaa ggacgtttca acggaacagt
    29521 tgaagttcat gaaggttcat tcaacgtaaa cggaaaagaa gttaaagtat tagctaaccg
    29581 caacccagaa gaattaccat ggggcgaact aggcgtagat atcgttctag aatgtactgg
    29641 tttcttcact tctaaatcag cagctgaaaa acatttaaca gctggtgcta aacgcgttgt
    29701 tatctctgct cctggcggaa acgatgttcc aacaatcgtt tataacacaa accacgaaac
    29761 attaactggt aaagaaacag ttatctcagg tgcttcatgt actactaact gtttagctcc
    29821 tatggctaaa acattgaacg acaaatttgg tgttgttgaa ggattaatga caactatcca
    29881 cgcttacaca ggtgaccaaa tgactctaga cggacctcat cctaaaggtg acttccgccg
    29941 tgcacgcgct gctgctgcaa acatcgttcc taactcaaca ggtgctgcta aagctatcgg
    30001 tttagtaatt cctgaattga acggtaaatt agacggagct gctcaacgtg ttcctgtacc
    30061 aacaggttca ttaacagaat tagtaacagt tcttgaaaaa gaagtaactg ttgacgaaat
    30121 caatgcagca atgaaagaag cttcaaacga atcttacgga tacaacacag acgaaatcgt
    30181 ttcttcagat atcgttggta tgacttacgg ttcattattt gatgctacac aaactaaagt
    30241 aatgacagtt ggcgacaaac aattagttaa aactgttgct tggtacgaca acgaaatgtc
    30301 atacactgca caattagtac gtactttaga atacttcgct aacttataag atagacaaac
    30361 gtacaagtgt aaattataga catgtacatt taaaagcggg gaagcaatcg cgcttcttcc
    30421 gctttttttg ataataagtt tattcaggag gaaataaaat ggctaagaaa acagtgaaag
    30481 atatcgattt aaaagacaaa aaagttcttg tccgtgttga cttcaacgtt cctttgaaag
    30541 acggcgtgat cactgacgac actcgtatca aagcagcatt gccaacaatc aactacgttc
    30601 tagaacaagg cggaaaagca atccttttct ctcaccttgg acgtgtaaaa acagaagaag
    30661 ataaagaagg aaaatcttta gctccagttg caaaacgtct aggcgaatta ttaggtaaag
    30721 aagtaacatt cgtacctgaa actcgcggag aacaattaga agaagcaatc cgcaacatga
    30781 atgacggcga cgttgtcgta ttcgaaaata cacgatttga agatattgac ggtaaaaaag
    30841 aaagcaaaaa tgatacagaa ctaggtaaat actgggcttc attaggcgat gtatttgtta
    30901 acgatgcttt cggtacagct caccgtgcac atgcttctaa cgtaggtatc gcttcaactg
    30961 gtatcccaac agttgctgga ttcttgatgg aaaaagaaat caagttcatc ggtgaagcag
    31021 tagaagaacc aaaacgacca atgattgcaa tccttggtgg cgcaaaagtt tctgataaaa
    31081 tcggcgtaat cgaaaacttg attcctaaag cagataaaat cttgatcggt ggcggaatga
    31141 cttacacatt ctacgaagca aaaggaatca agatcggtaa ctctctagta gaagctgata
    31201 aagtggaact agcaaaagaa ttaatcgaaa aagcaggcga caaacttgtc ttgccaatcg
    31261 acaatgtctg tgcaccagaa ttttcaaatg atgttgaaac acaagtaatc gaaggagaca
    31321 ttcctgatgg tttgatggct ttagacatcg gcccagcatc agtaaaatta tttgctgata
    31381 cattacaagg tgcaaaaaca gttgtttgga acggacctat gggtgtattt gaaatgagta
    31441 actttgctaa aggtacaatc ggtgtttgtg aagcaatcgc taacttagaa ggcgcaacaa
    31501 caatcatcgg tggcggagac tcagccgcag cagcagaaca attaggattt gctgacaaat
    31561 tcacacacat ctcaacaggt ggcggcgcaa gcttggaatt gcttgaaggt aaagaattac
    31621 caggactagc agcaatcaac gataaataaa cgaccaagtt tgctcaattc tgacgagtaa
    31681 tcgatcaaaa ggtgaaggga gttttcatga ctccctagcc atttttcaat aaattaagga
    31741 gtgtgttcct caatgcgtaa acctattatc gcaggtaact ggaaaatgaa caaaactgca
    31801 tcagaagcaa aagaatttgc tgaagcagta aaaaacaaaa tcccttcaaa tgacaaagtt
    31861 gattcagtca tcggatctcc tgcattattc ttacaagaat taatgacatc agctgaaggt
    31921 acagaactaa aaatctcagc acaaaactgc tactgggaaa actcaggtgc gttcactggt
    31981 gaaacttcac cagcagctct tgctgatcta ggtgttcatt atgtgatcat cggacattca
    32041 gaacgtcgtg aatatttcca tgaaacagac gaagacatca ataaaaaagc gaaagctatc
    32101 tttgcaaatg gtatgacacc aatcctatgc tgtggcgaat ctttagaaac ctatgaagca
    32161 ggcaaaactg ctgaatggat cgaaggacaa attacagctg gtttagctga tctttcagct
    32221 gaacaagtgt caaacatggt tattgcttat gaaccaatct gggcaatcgg tactggtaaa
    32281 tcagctgacg cacaaatcgc tgacgaaatt tgtggagttg tacgtcaaac agttgaaaaa
    32341 ctttatggaa aagaagtatc agaagcagtt cgtatccaat acggcggttc tgtaaaacca
    32401 gaaaatattg ctgaatacat ggcaaaagaa aatgttgacg gtgctttagt cggtggcgca
    32461 agcttgcaag cagattcatt cttgtcattg ttagacgctg taaaataatt gttgaaaatt
    32521 tgcgaaaaat gcaagaaatg tttgcactaa tgagcaaaat taactaaaat cgtatatgag
    32581 ggaaatccct taggaaaaaa caaactcaaa ggagagacaa aacatgtcaa ttattactga
    32641 tgtttacgct cgcgagatct tagactcacg tggtaaccca acaatcgaag tagaagtata
    32701 cacagaaagc ggagcttttg gccgcggaat ggttccttca ggtgcttcaa ctggtgaata
    32761 cgaagcagtt gaattacgtg acggagacaa agctcgttat ggcggtaaag gtgtaactaa
    32821 agctgtcgac aacgtaaaca acatcattgc tgaagcaatc attggctacg atgttcgtga
    32881 ccaaatggct atcgacaaag ctatgatcgc tttagacgga actcctaaca aaggtaaatt
    32941 aggtgcgaac gctattttag gtgtttctat cgctgttgca cgtgcagctg ctgactacct
    33001 agaagtacct ttataccact acctaggcgg attcaacaca aaagtattgc caacaccaat
    33061 gatgaacatc atcaacggcg gatcacatgc tgataactca atcgacttcc aagaattcat
    33121 gatcatgcct gttggcgctc caacattcaa agaagctcta cgtatgggtg ctgaagtatt
    33181 ccacgcatta gcttcaatct tgaaagctcg cggattagca acttctgttg gtgacgaagg
    33241 tggatttgct ccaaaccttg gttcaaacga agaaggtttt gaagtaatca tcgaagcaat
    33301 cgaaaaagct ggctatgtac ctggtaaaga cgttgttctt gctatggatg ctgcttcttc
    33361 agaattctac gacaaagaaa aaggtgttta cgtactagct gattcaggcg aaggcgaaaa
    33421 aacaactgac gaaatgatca aattctacga agaattagtt tctaaatatc caatcatctc
    33481 aatcgaagac ggattagacg aaaacgactg ggacggattc aagaaattaa ctgacgtatt
    33541 aggcgacaaa gttcaattag ttggtgacga cttgttcgta acaaacactc aaaaattatc
    33601 tgaaggtatc gaaaaaggaa ttgctaactc aatccttatc aaagtgaacc aaatcggtac
    33661 attaacagaa actttcgaag ctatcgaaat ggctaaagaa gctggctaca cagcagttgt
    33721 atctcaccgt tctggtgaaa cagaagattc aacaatctct gatatcgctg ttgcaacaaa
    33781 cgctggccaa atcaaaactg gttctctatc acgtactgac cgtattgcta aatacaacca
    33841 attactacgt atcgaagacc aacttggtga agttgcagaa tacaaaggtt tgaaatcttt
    33901 ctacaactta aaaaacaaat aattcttttc taatgaagat acatgatcga ctattgaaag
    33961 atagtcgagt atgatcactt agttaagtaa tcgaactctc tacttgctga aatatcagca
    34021 ggtagagagt tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag
    34081 aaaagatcat ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa
    34141 ggatagaaca gtagaatagg aatctatgag caagtcctct cgtataaata aaataggagg
    34201 gaagtagcag catatttttc ataaagtggc tctctttctt catttttact ttggttgctc
    34261 ctatttgaaa attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta
    34321 gatgaaaaaa gaagaaaaaa tcgcgatttt acaagagatc atccgaatta aatcagtgaa
    34381 tggaaacgaa ggtgaagtag cagcctattt gaataagctt ttggcaaagc atgacataac
    34441 aggagagatt gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg
    34501 ccaatcagga aaagtgttag gcctatctgg gcatatggat gtcgtggcgg ctggtgatga
    34561 atcgtcttgg acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg
    34621 tggtgctaca gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa
    34681 agagtcagga aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt
    34741 gggtgaacta ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt
    34801 gatcatcgga gagccaacta attacagttt gatgtatacg cacatgggat caatcaacta
    34861 tacagtcacc tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc
    34921 aatcaatcac ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga
    34981 aacaatcgag aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg
    35041 gaatcaagtc aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc
    35101 agaataccca aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca
    35161 agagacagac tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga
    35221 tccagattct cctttgattc attgcattca gcaacaattc agccagccat tacctttagt
    35281 tggagcggca gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt
    35341 tgtcgtcttt gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat
    35401 cgataattat ttggatatga ttgaaaaata tcaaggaatt attttatcct acttagcgta
    35461 agattttttt aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc
    35521 tgtttattca gcctattttt ctttttgaat atatcaagcg ttgtggcaac atagtgatga
    35581 ctaatgaaac aacgacgata gacaacaaac gatcaaggta gtctgtacct gcctgtacca
    35641 agatcacact tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg
    35701 atcctgagga ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc
    35761 caggtaccga caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga
    35821 gatatcttgg caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat
    35881 acagagaaaa aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca
    35941 ttccaggtac aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga
    36001 ttggcaaacg aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag
    36061 ccatcactgt aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt
    36121 aacctttcca aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac
    36181 tttcggaaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
    36241 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccttgagt ttccacagca
    36301 gtgaagcttt tgaaaccttc acagatggcc ccctcgataa aataagcaac agctaacgga
    36361 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
    36421 gtgatcgctt ttagatactc tccctcttca ggattcattt gacagcagta ttcgaggata
    36481 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
    36541 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
    36601 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
    36661 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
    36721 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
    36781 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
    36841 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
    36901 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
    36961 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
    37021 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
    37081 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
    37141 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
    37201 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
    37261 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
    37321 gataatagtt agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
    37381 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
    37441 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
    37501 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
    37561 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
    37621 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
    37681 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
    37741 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
    37801 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
    37861 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
    37921 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
    37981 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
    38041 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
    38101 catcagctac gagacagctt ggcagcaacc atctgccttt attggtcatt catggtgaaa
    38161 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
    38221 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
    38281 cctattggac agtgattttt tcatttattg aacaaagaat aacgatgaat taattttctc
    38341 ttcttttttg taagtaattc gaggataaat aattgtttat agatcaggag actgctaaaa
    38401 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
    38461 gaaaaaaatc gtcttgcttt attcaatgat gaacaacaag agatcggaga aatgacatgg
    38521 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
    38581 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
    38641 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
    38701 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
    38761 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
    38821 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
    38881 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
    38941 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
    39001 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
    39061 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
    39121 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
    39181 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
    39241 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
    39301 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
    39361 tatgatattt tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
    39421 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
    39481 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
    39541 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
    39601 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
    39661 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
    39721 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
    39781 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttcg atggtatcct
    39841 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
    39901 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
    39961 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
    40021 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
    40081 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
    40141 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
    40201 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
    40261 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
    40321 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
    40381 cttattcaga agaagaaata caagaaacgg atctttacgg cgttgttatc ccgaaagata
    40441 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
    40501 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
    40561 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
    40621 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
    40681 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
    40741 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
    40801 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
    40861 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
    40921 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
    40981 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
    41041 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
    41101 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
    41161 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
    41221 tttcttttga ggatcgcgaa gcaaaaatcc ttgttgatgc tgaaggacac ccaaatgata
    41281 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
    41341 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
    41401 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
    41461 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
    41521 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
    41581 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
    41641 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
    41701 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctac ggaacttcct gaaattgcag
    41761 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
    41821 agaaagcaga attcatgatg gacaaagtgg gggaagaatt cgatgggatc atcagctctg
    41881 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agagggattg atccatatca
    41941 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
    42001 gaactggtct gacttttaaa atcggacaga aagtgcgagt aaaagtagtc aaagctgatc
    42061 cagatgaacg agcagtagat tttgaacttg tttccgcaga agaggttaca gcgttggaac
    42121 ggccaaaaac acgaaatgga aaatcaggaa aacgcgaagg aaaacaagca ggaaaacgcc
    42181 cagaaagaaa tggcaagaag aacaaacgat atagcgatag taagcaaacg ttcggtaaga
    42241 aaaaagggaa gaagccattt tacaaaggtg ttaaaaagaa gaaaaagaaa aaataaaatg
    42301 agttggagga aacagctatg ccaaaaggcg agggaaaatt aattgcacaa aacaagaaag
    42361 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaaccg
    42421 agatcaagtc gatacgaaac agccggatca atctaaaaga tggatttatt cgcgtccgca
    42481 acggagaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
    42541 atcatgatcc gttgcgcacg agaaagttat tattacacaa aaaacaaatc atccggcttg
    42601 aaaatgaatt gaaaaatact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
    42661 gctatgccaa ggtattgatt ggtctggcga aagggaaaaa atcttatgat aaacgggaag
    42721 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
    42781 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
    42841 ttagcattta atctgataaa acatgattat cataggtttt ttcttagatc aggagcaccg
    42901 tgtataaaga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
    42961 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
    43021 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
    43081 ggattggatg aacgctcgct aacgttccac attggaccaa tttggtttga tggtacagtt
    43141 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
    43201 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
    43261 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
    43321 tttacattgt ttttatttgt ttttgttgca aacaacatcg gtttagtaac aaaaatcgtt
    43381 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
    43441 ttggcattta taatgatctt acttactcac ttatttgggg tgaaaaagtt aggcttcaaa
    43501 ggatatttcg taaattcgtt cttgaaacca tatagtttta tgtttccgat gaaactgatt
    43561 gaagaattta caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
    43621 gaagttttgc taaccttgat tgcaaaaatg gtaataagct taggctggtt ctcattgccg
    43681 ttagcaattc cattagagat ggtctggatc gcattctcct tgtttatcgg aagcatccaa
    43741 gcatttgtct tcgttacttt atctatggtt tatatgagcc ataagattga agtggaagaa
    43801 taatattcgt tttaaaaaca aactactatt tatctaattt aggaggaaaa caattatgaa
    43861 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
    43921 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
    43981 ccgtacaaca atgtttatcg gggtagctct agtcgaagct gttcctatct taggggtagt
    44041 tgtcgccttg attctagttt ttggtgttta attgaatcag tattaaggca gaaacaattt
    44101 ggtttgttac tgctttttta catgaaaact agaagtactt caaagaaagg aaggctcgcc
    44161 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggaa atgtcatcgt
    44221 tgtcagcggc tcatttttga tactactggc tctcttgaag cattttgctt ggggaccaat
    44281 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttggatt ctgcagaaca
    44341 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
    44401 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
    44461 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agtaaagccc aagctgatat
    44521 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gatgttgcag aactatctct
    44581 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcacg aatcattaat
    44641 caatcaatat atcgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
    44701 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaataaagca gaagaaattt
    44761 atcaagatct cgtaagctta cgtcaaatct atgaacaagt acctggatta ggtaatatgt
    44821 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgttagtg
    44881 gctatgatgg aatcgttaaa aactttttag aagttgttta tgcgtataac cgaatggatg
    44941 atcttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa ggtttgctgt
    45001 tgggaagtgt cacaacagca gttccacttt cagacgaaca acgaatacaa ttagaaaaga
    45061 atgttgcaaa aacaatgaac tatcaatcag tcgaattaaa acaaatcgtt gattcttcta
    45121 ttattggtgg cgcgattgtt gaagctaacc atcgagtaat cgacggaagc attcgcacgc
    45181 aattagaaaa tatgcgtaat caattaaata gatagtacaa agaggtaggt gaatcgaatg
    45241 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
    45301 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
    45361 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga
    45421 atggcgcaaa acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
    45481 attcgtgaag gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag
    45541 gccttgattg gacgggtagt taatccgcta ggtcagccaa ttgacggact aggtgaaatc
    45601 gttactgaca aagctcgtcc agtagaagca atggcaccag gcgttatgca acgtaaatct
    45661 gttaacgaac caatgcaaac aggtctaaaa gcaatcgacg ctcttgtacc aatcggacgc
    45721 ggccaacgtg aattagttat cggtgaccgt aaaacaggga aaacttccat tgcgatcgat
    45781 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
    45841 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
    45901 atcgttgtga atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct
    45961 ggtactgcaa tgggtgaaga gttcatgtac aacgggaaac atgtcttgat catttttgat
    46021 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
    46081 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
    46141 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    46201 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    46261 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    46321 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    46381 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    46441 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    46501 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    46561 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    46621 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    46681 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    46741 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    46801 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    46861 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    46921 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    46981 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    47041 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    47101 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    47161 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    47221 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    47281 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    47341 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    47401 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    47461 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    47521 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    47581 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    47641 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    47701 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    47761 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    47821 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    47881 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    47941 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    48001 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    48061 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    48121 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    48181 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    48241 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    48301 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    48361 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    48421 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    48481 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    48541 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    48601 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    48661 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    48721 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    48781 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    48841 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    48901 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    48961 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    49021 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    49081 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    49141 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    49201 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    49261 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    49321 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    49381 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    49441 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    49501 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    49561 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    49621 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    49681 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    49741 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    49801 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    49861 gaataaggga ctgcgacaag ac
//
LOCUS       DAFEHS010000013        48955 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFEHS010000013
VERSION     DAFEHS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.013"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(148..492)
                     /locus_tag="LP327_000797"
     CDS             complement(148..492)
                     /locus_tag="LP327_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL1724939.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(485..919)
                     /locus_tag="LP327_000798"
     CDS             complement(485..919)
                     /locus_tag="LP327_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBL1724940.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1057..1794
                     /locus_tag="LP327_000799"
     CDS             1057..1794
                     /locus_tag="LP327_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1724941.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1787..2998
                     /locus_tag="LP327_000800"
     CDS             1787..2998
                     /locus_tag="LP327_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1724942.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3202..3984
                     /locus_tag="LP327_000801"
     CDS             3202..3984
                     /locus_tag="LP327_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBL1724943.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4054..4716
                     /gene="trmB"
                     /locus_tag="LP327_000802"
     CDS             4054..4716
                     /gene="trmB"
                     /locus_tag="LP327_000802"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBL1724944.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4814..6568)
                     /gene="ade"
                     /locus_tag="LP327_000803"
     CDS             complement(4814..6568)
                     /gene="ade"
                     /locus_tag="LP327_000803"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBL1724945.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6700..7629
                     /gene="tadA"
                     /locus_tag="LP327_000804"
     CDS             6700..7629
                     /gene="tadA"
                     /locus_tag="LP327_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBL1724946.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7586..8635
                     /locus_tag="LP327_000805"
     CDS             7586..8635
                     /locus_tag="LP327_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBL1724947.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8632..8910
                     /locus_tag="LP327_000806"
     CDS             8632..8910
                     /locus_tag="LP327_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBL1724948.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8885..9355
                     /locus_tag="LP327_000807"
     CDS             8885..9355
                     /locus_tag="LP327_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBL1724949.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9372..9674
                     /locus_tag="LP327_000808"
     CDS             9372..9674
                     /locus_tag="LP327_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724950.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9691..10062
                     /locus_tag="LP327_000809"
     CDS             9691..10062
                     /locus_tag="LP327_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBL1724951.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            10034..10393
                     /locus_tag="LP327_000810"
     CDS             10034..10393
                     /locus_tag="LP327_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724952.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10565..11572
                     /locus_tag="LP327_000811"
     CDS             10565..11572
                     /locus_tag="LP327_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL1724953.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11597..12781
                     /locus_tag="LP327_000812"
     CDS             11597..12781
                     /locus_tag="LP327_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBL1724954.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13218..14801)
                     /locus_tag="LP327_000813"
     CDS             complement(13218..14801)
                     /locus_tag="LP327_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL1724955.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15379..15522)
                     /locus_tag="LP327_000814"
     CDS             complement(15379..15522)
                     /locus_tag="LP327_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBL1724956.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15745..16224)
                     /locus_tag="LP327_000815"
     CDS             complement(15745..16224)
                     /locus_tag="LP327_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL1724957.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16297..16581)
                     /locus_tag="LP327_000816"
     CDS             complement(16297..16581)
                     /locus_tag="LP327_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724958.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16659..16853)
                     /gene="ssrS"
                     /locus_tag="LP327_000817"
     ncRNA           complement(16659..16853)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LP327_000817"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16925..18211)
                     /locus_tag="LP327_000818"
     CDS             complement(16925..18211)
                     /locus_tag="LP327_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBL1724959.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18349..19020
                     /locus_tag="LP327_000819"
     CDS             18349..19020
                     /locus_tag="LP327_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBL1724960.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18986..19474
                     /locus_tag="LP327_000820"
     CDS             18986..19474
                     /locus_tag="LP327_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBL1724961.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19487..20434
                     /gene="prmA"
                     /locus_tag="LP327_000821"
     CDS             19487..20434
                     /gene="prmA"
                     /locus_tag="LP327_000821"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBL1724962.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20435..21199
                     /locus_tag="LP327_000822"
     CDS             20435..21199
                     /locus_tag="LP327_000822"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBL1724963.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21432..23645
                     /locus_tag="LP327_000823"
     CDS             21432..23645
                     /locus_tag="LP327_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBL1724964.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23656..24102
                     /locus_tag="LP327_000824"
     CDS             23656..24102
                     /locus_tag="LP327_000824"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBL1724965.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24273..25532
                     /locus_tag="LP327_000825"
     CDS             24273..25532
                     /locus_tag="LP327_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBL1724966.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25621..26568)
                     /locus_tag="LP327_000826"
     CDS             complement(25621..26568)
                     /locus_tag="LP327_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL1724967.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26962..28254
                     /locus_tag="LP327_000827"
     CDS             26962..28254
                     /locus_tag="LP327_000827"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBL1724968.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28269..30038
                     /gene="aspS"
                     /locus_tag="LP327_000828"
     CDS             28269..30038
                     /gene="aspS"
                     /locus_tag="LP327_000828"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBL1724969.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30147..31022
                     /locus_tag="LP327_000829"
     CDS             30147..31022
                     /locus_tag="LP327_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL1724970.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31288..33171
                     /locus_tag="LP327_000830"
     CDS             31288..33171
                     /locus_tag="LP327_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL1724971.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33246..33530)
                     /locus_tag="LP327_000831"
     CDS             complement(33246..33530)
                     /locus_tag="LP327_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL1724972.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33520..34254)
                     /locus_tag="LP327_000832"
     CDS             complement(33520..34254)
                     /locus_tag="LP327_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBL1724973.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34383..35006
                     /locus_tag="LP327_000833"
     CDS             34383..35006
                     /locus_tag="LP327_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBL1724974.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35198..36319
                     /locus_tag="LP327_000834"
     CDS             35198..36319
                     /locus_tag="LP327_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBL1724975.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36326..39454
                     /locus_tag="LP327_000835"
     CDS             36326..39454
                     /locus_tag="LP327_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBL1724976.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNELLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39473..42667
                     /locus_tag="LP327_000836"
     CDS             39473..42667
                     /locus_tag="LP327_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBL1724977.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42813..43520)
                     /locus_tag="LP327_000837"
     CDS             complement(42813..43520)
                     /locus_tag="LP327_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL1724978.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            44285..44683
                     /gene="spxA"
                     /locus_tag="LP327_000838"
     CDS             44285..44683
                     /gene="spxA"
                     /locus_tag="LP327_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL1724979.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            45015..45668
                     /locus_tag="LP327_000839"
     CDS             45015..45668
                     /locus_tag="LP327_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBL1724980.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            45894..46892
                     /locus_tag="LP327_000840"
     CDS             45894..46892
                     /locus_tag="LP327_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBL1724981.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            47068..48876
                     /gene="pepF"
                     /locus_tag="LP327_000841"
     CDS             47068..48876
                     /gene="pepF"
                     /locus_tag="LP327_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBL1724982.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        15918 a         8558 c        10284 g        14195 t
ORIGIN      
        1 tttatatatt aactatacca tatcccattt cctttaaaaa cgttgtcgtg gtagtttcaa
       61 acaaaattca aaatttcatc acattcctag gaaatacgca aacttttctc acttatatag
      121 ttaaatctgt tgagaaaata atcattatta tatttgttca ggatactctg gcagatcagc
      181 ttttagtttt tctacttgtt cattgatttg tgtgatacgt ggttcggctt ggaatttaaa
      241 atcttcgata tcttttttga ttgcttcttg gaaagcagga atcacagatt cagctgtttc
      301 ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg ttatttaaat catttgtcag
      361 agttgttgct tcttccaatt cttcgatcag tttctttctt gtttcattcc cgctcctggg
      421 cgccaataac agacctccga cagtgccagc agcagctcca aataacagtc ctttgaaaaa
      481 gtttttaatc atcgcaggac acctgcttta cgattgtttc ttgaatagct tttagttctt
      541 ccgatccata actatcttga tgattgacaa agtgaataga aaaatcatcc tctttgctgt
      601 agcgagggac taaatggata tgtgaatgaa aaacagattg ataggctagt tcacggttgt
      661 tattcaagat attaagacct ttcatttctg gaaaagcttt ttccaatgct tgagcaatct
      721 taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc atattcaaaa atatctgcta
      781 catgacgttt aggcacaact aatgtgtgtc ctttcgttgc ttgagagatg tctaaaaaag
      841 catagacctt gtcgtcttca tatattttat agcttgggat ttcttgattg atgattttgc
      901 aaaaaataca gttttccata taaaacatcc tttctataaa gaataaatac ttcagcttaa
      961 ctttaccata agttaaagct attgtaagga aaaaaacaaa ataccgtcat tttcttcgtg
     1021 gtataataaa taaaaataaa tggcggagga aaaagaatga gtttaattat cgatcatgtc
     1081 acaggggggt acggacatct ccctgtgtta aaagatatca gtttcgatgt acagcctgga
     1141 gagatagtag ggttgattgg actgaacgga gcagggaaaa gtacgaccat caagcacatt
     1201 atcggtctgc tggaagcttc aaaaggaacg atagaaatca atcatctatc cttaaagaaa
     1261 aatacggaag aatatcgcaa acaaattggg tatattccag agactccttc tttatatgaa
     1321 gaattgacat tgagagaaca tatcgaagtc actgcgatgg cctacgatat acctatggat
     1381 atagcaatga aaagagcaga aaagttgttg gagacttttc ggttatctaa tcgtcttgac
     1441 tggttcccag ctaatttttc aaagggaatg aaacagaaag tgatgatctt atgcgccttt
     1501 ttgatcgagc cttctttata tatcatcgac gaaccatttt taggcttgga tccactagct
     1561 atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag aaggagccgg tattttgatg
     1621 tctacgcata ttttagctac tgcagaaaaa tattgtgatc gttttgtggt cttacataac
     1681 ggagagatac gagcagacgg ttctcttgag atgttgagaa aagaatttca gctgccggat
     1741 tcttctttgg acgaaatcta tattgcttta acaaaggagc aggcacatga gtagtttttt
     1801 tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa tatatgcgtt atgtattgaa
     1861 cgatcatttt gttttagttt gtcttttctt gattggcggt gtgggattat attattctaa
     1921 ttggttgaaa accctgcagc caccgtttgc aattggaggg ctcataatct ctgtattttg
     1981 gatgctctgt ttattcgtgg gtaagatggc tacttttgca gaggcagcag atattgtttt
     2041 tttacttcct aaagaaaaag aaatgcaaac ctatttggaa cgagcttttc gctattcttg
     2101 catttttccg ttgatctttt tgatattggc atgcggattg gccatgccat tagtggtggt
     2161 tagtacggag caatcctttt cgatgttttt cgtctatctg atcatattat ggagtttgaa
     2221 attcagtcat ctgcaaatca agagagcaga attgtttcga ctagatcaac aagttattcg
     2281 ttattggaaa ttaggctggt ttttttcttc tctgatcttt ttgtttctat cattttattg
     2341 gctaatatgg gcaggaatag caggtgcatt agtacaggca agtctatatt actggttttt
     2401 gtggaagcaa caagaaaaag gattggattg ggagaaaatg gtgcatgtag aagaacatag
     2461 gttgcatcag atctatcaat ttatcaattt attcacagat gttcctgaaa ttacggcaaa
     2521 gatcaaaaga agacgctatt tggatcctat attgactcat attaaaagag aatcaaaaaa
     2581 cacttatctt tacttgtttg caagaagatt ggcaagaggt tctgatttta gtggtcttta
     2641 tttccgatta gtaattatag gtgggttatt gattgccgga gtagtcgact tttattttag
     2701 tctgattatc ggtttcttat ttttatattt gatcggtttc caactgttac ctttagcgaa
     2761 ccagttccgc tatatggttc tcgttcagct ttatccaatc cataagaatc agaagaagca
     2821 ggcgattcag tatctactca catgggtatt aattatcgcg tcggttattt ttggtttgat
     2881 tgctgcagtc gtattagatg ggcagcagcg atgggttccg ctagcggctt atttgattgt
     2941 tactatgctc tttgttaaac tatatgtacc aactcgtttg aagaagatgt cagactgaaa
     3001 acaaagctgt gtagggtaaa aatgagaatg aaaaatagag cgttgacagt aagaatgctg
     3061 ttcgttctat tttttttgag aaaagctggt tttttgaatg gcaattaaat gaaaaagaac
     3121 gtatatgtgc ttgacgtgta gtgttcaggc gactagaata ataagtactg caaaacaaag
     3181 agtaagagga cgggagaatg catgatggag tttcagttag accaagagtg gcggttacgt
     3241 cccatcaaag gcgacacagg aaaaacatac ataggcatgc gtgatgagga caaagtcttt
     3301 attaaacgaa acacgactcc gatgttggct gctctttcaa gggaaggaat tacaccaaaa
     3361 cttgtttgga caaaacgaac ggggaatggc gatactttga cagcacaaga gtggctggaa
     3421 ggtcgcttgc tgactcctaa tgagattggt caaagaaacg acgtgattga tgttttgtac
     3481 catttacatc attcaaattc tcttaaaacc atgctgaccc gtatcggtgg tcgtgtcatg
     3541 acgccggaga agatgctgca aaaatatgaa aaagaattac cggaagaact ccagaaaaat
     3601 caatttctgc aaattgtcca tcgttttctt cgtgagcaga tcccgtcaat agataaggaa
     3661 aacttatcag tagtacacgg agatgtaaac catcgtaatt ggttagtatg taaaaattat
     3721 ttatatctag tcgattggga ttcaatcatg tttgcagacc ctgcggtgga tattgcaacg
     3781 attttaggaa attatgtgcc actgtctagc tggaatcagt ggctgatcag ttatggcata
     3841 cgcccgactg acgaaatgct tgaaaaattt cattggtatg cggccatgaa tttgctccaa
     3901 gaaatcacca gatattgcaa acgtgaagat catcgacgga tgaacgaaga aattttacaa
     3961 ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc tgcggagatt tggccagacc
     4021 tttttaatta gaggaaagga atggagataa ataatgcgtg tccgaaatcg tccgggagca
     4081 gcggaaatgt tagcggctca cccgaatttt gtaattagtg atccaacatt gtggaaggga
     4141 aaatggaatg aattgtttga aaacgatcat ccaatccaca ttgagattgg tatggggaaa
     4201 ggccagttca tcactggaat ggcaaaagcc catcctgaaa tcaattatat tggagtagaa
     4261 atgcaggtca gtgttgtttc gattgcacta gacaagttga tcgaacaacc gcttcctaac
     4321 ctgaaacttc tacatgtgga tggatcagcg ttgacagagt atttcgctga cagcgaagta
     4381 gatcaaatct atctgaattt ttccgatcca tggccaaaaa aacgtcatga aaaacggcga
     4441 ttgacatata agacttttct tgctgtagat gaacagattc ttcgtccaaa tggagaaatc
     4501 catttcaaaa cagataatca aggattgttt gagtattctc tggcaagttt ttctcaatat
     4561 ggcatgatcc tcaaacaggt atggttagat ttacatcaaa gtcaatttga aggcaatatt
     4621 atgacggagt atgaagaaaa attttcttca aaaggtcaac gtatttatcg agtagaagct
     4681 caatttcaag ataagaccaa aaagaaaagt ctgtgaaact aactttctgc tttcataaaa
     4741 acattaatat ccataaataa atcccagaaa atgaacatta cagatgtaat gtattttctg
     4801 ggatttattt gatttaaaat gtatttattt catcggtttt tccacaaggt ggaatcaatt
     4861 ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt caattcgggt atgacagata
     4921 atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt agctgctcct agttgatgtg
     4981 ctttagttat taattcattc aactgttcat agacgatcga aaaaggttcc tgtgtcatga
     5041 ttccgcctat aggaagagaa acacaagcta tttcctgctg tttgttgaca acggtcatcc
     5101 cgccaccttt ttgaatcaaa agattagcag catacagcat atcctcgtcg tttgtcccaa
     5161 cgacaactag attatgcgag tcatgggcaa tcgttgtcgc tatcgctcct tgtttcagct
     5221 gaaatccttt cactatgcct aatccaatgt ttcccgtttg atggtgacgc tcgatcaccg
     5281 caatctttaa ttggtctttt tcgattgaag gaatgaatcg ttcgccttgt cggtccactt
     5341 tttctaccaa atctttagtc actagactat tcgggataat ttcgatcaca tgggcttgtg
     5401 aagaagaaag aagcagctct aacgaatgtg gatttaatgg ttgagcgttt agttttggca
     5461 gacaacacgt atatgaattt gaatgatcag caaataaatg gtcttggagt tgcccagatt
     5521 gaacaactaa tgttccctta tagaaaactt catgaattgg cagcttggta agatcgtctg
     5581 ttaagaagaa atcagcttga tacccagggg caatcgcacc tagtgttttc aaatcaaaac
     5641 attctgctgc attcaatgtt gccatctgaa tggcttgtaa tggatcgatt ccaagtgcaa
     5701 tagcttgacg gacgatatga tcgattgatc cttcttttac taaatcattg atcagtttat
     5761 catcggtgac aaagaagcat cttcttgaat tttcaggtgt gatcgccggt agtagcgatg
     5821 ccagatcttt tgcaacggtc ccttctcgaa ccattaaata cataccaaga tccaaacgtt
     5881 ctttagcttc ttttgcagtt gtagcttcat gatccgtccg aatacctgct gcaggataaa
     5941 catttaactc ttccatgccg ataccagctg catgcccgtc aatttttttc ttatgctggt
     6001 gcatcaattg gatcttttct aggatgtctg tttcattatt tgctactgct tgatagttca
     6061 tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa aggggctaat ttttttgcat
     6121 ttaaatttgc accattggtc tcaaaagggg ttacaggtac acaagaaggt agcataaaaa
     6181 agatattcat gagtgtttgt cttgcatctt ctatcataaa ctcaagacct gtactcccag
     6241 caatgtttcc tatttcatgg ggatctgtca ctacagtagt aactccatga agcaaggata
     6301 cttttgaaaa ttcgcttggt gataaaagtg agctttcgat atgcacatgg ccatcgatga
     6361 atcctggcac tagatattgc ccattagcat tatgtacgct ttttccttcg tattcaccga
     6421 ttcctacgat cattccttcg cagattgcta catctttttc catgatttct ttcgtaaaga
     6481 cgttgacgat cttagcatgc ttgatcacta ggtcagcagg ctttttttta cttgctactt
     6541 cgatatattt ccttaatgtt gtttccaatg gagtgcctcc caagataaat ttttataagt
     6601 ataacataaa ttcggttttt gccaatattt ttttgtaaat tcattttatt tgaaacaaaa
     6661 agatcaaaaa gacgtatgtt ttttgtggag gggagaataa tggaaataaa acgattatcg
     6721 gatcaaatga taagtgaaag ttttgccaaa catgcccaag acgtatatat attgccagct
     6781 tcagaagtat tttacatcta ttttcgctat ggaaaaaaac gaacattctg gaagaaagtc
     6841 tcttcggcag atggacagca actgatctca agatttaagt atttgggaca gatggatatc
     6901 ggagaaaaaa gaaaagctca gttaggtgca tttacttatt ctttagaaaa acaggaagtt
     6961 cgtctgcggt tgtcttctgt aggagattac cttcaacgag agagtttagt gattcggcta
     7021 ttatacggta cttttgaaac accccatcgt tgctttagtt ctcgagattt tgcaactatt
     7081 ttacagaaaa ccgtcagacg tggattatac cttttcagcg gaccagtcgg atcaggcaag
     7141 acttctctaa tgtatcggtt ggctttggaa gaaaaattgc aagtgattac tatcgaagat
     7201 cctgtagaga tagaagaacc gttttttctg cagctacaga tcaatccgaa aatagcacag
     7261 acttatgata cacttttgag actcgcatta cggcatcgtc cagacttgct aatcattggc
     7321 gagataagag atgaatcaac tgcacatgct gcggtaagag cagcactaac agggcaccgg
     7381 gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat tggtcagaat ccatgaactg
     7441 acaaagaaaa aagaagagct atctgaatgt ttagctggtg tcatttatca agaacttttg
     7501 gaagatgcca atcaccatcc ttctgtttta tggagttatc aatttatgga ggaagaatca
     7561 gtaaaaaaat catgggagga aagttatgaa gaagctcagg aaaatatttg gcagtcagaa
     7621 ctattctaaa ggattaacaa gaagccagca aaaccagttt gcacaaacca tgggagaact
     7681 gcttttatct ggttttagtc tgcaagaatc cctttattta ttactcacga tccgttcgtt
     7741 tccacctgtt attttggcaa atatccaaca tcagctaaag gaagggcaag cgtttagtga
     7801 ggtactttcg caaataggat atggagcaga gcaactggta cagatcgagt tagctgaacg
     7861 tcatgggaat ctgccgacaa ctttgttgga aattgccaaa cagtttcggc ttgttgaagg
     7921 atttcgcaat gatttgaaaa agctgcttgc atatccgatg ctattgctca cctttttgct
     7981 aggtatcttg tttagtatgc ggttgttcgt tttgcctcag cttattggtt caggaatggt
     8041 catgcaagag cattggggta ttcagctgat acaagtcttt cattggtatg ttctactatt
     8101 catcctagcg gttactctat tggctttact agtaaggtat cgtctgatga agttgtctcc
     8161 tgttgatcag agtgaatgga taagcagaca attattgatt ggaccgatat attccttgta
     8221 tcaatcggct tatttgtcac tagagattgg aaagcttttt catgaaggtt tcgaattaag
     8281 gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt ctgattcagc atcttgcctc
     8341 taagttgatt caaggattgg aggcaggtat gtcattggct gagcagttcc agaattatcg
     8401 ttttctatca gaagaattca gtaaaattgt tttacaggga gaggcaaagg gaagtttagg
     8461 taaagaactg attttttaca gcgaatggac aagaaaacag cttttcaaac gtcttcatca
     8521 ctggatgcat tttattcagc caatcatttt tttgggcgta gcggctttga ttttattagt
     8581 ttatgccgct gttttattac ctgtttacgg aagcatagag gaggttttgc catgaaaaag
     8641 agaaaaatat cttattatag cggttttaca ttgatcgaaa tgctaatcgt cttactgatc
     8701 atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc gttataggaa tcatgtagac
     8761 caagaaagtc gtgaagcgat catccagttg gtagacacac aaaaagagct ctatgcgctt
     8821 caaaataatg gaagagttcc tactgtggag gaattgttga atgaaggcta tatcaaacga
     8881 gaacatgcag aaatctatca gcggccttga aggtaaggtt ttagcatcag gatatacgtt
     8941 gtttgaatca ctcttactga ttttattact tacaacgatc ctttcttttc cagttctcgc
     9001 attttctgca tggaagaatg aattggcagt ctatcaattt ttcggccaat ttgaaaaacg
     9061 aatctattct acgcaaaaaa tcgctattgt gaatcagatc caaacaggct tttattggaa
     9121 tagtgaagag aatcaaatca tttttcatgt tccgaatcca gataaaataa attgggaagt
     9181 attagatatt cctgatgaga ttaccttgaa acgccatgct tctattactt ttgctgctag
     9241 aaccgggaat gaaagttcac taaaagctta tcaattttat tgggaagaaa aagagcggac
     9301 gattacgtat caattccaga tagggagtgg tcgctataca aaaagaattg aataaaacag
     9361 gatttgtttt gttggagtcc cttacagcgc ttatgatcgc ttctgtctgt gtctacttag
     9421 tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa aagtggggag gaactgctat
     9481 ttattcgaat gatttatgaa gatatcaaga cgcagcggat gtatcacaaa agaccagagc
     9541 gaagggaaga aatgatccca tacgatatcc aaattgtcga taataatgga gtaatcgaaa
     9601 aagtgtttgt caatatggag aaagaagcga tcagtattga aaaaacaagt aattccagca
     9661 tttacgctga ttgaatgtct aattggactg atgatattga gcagtctatt gctcacattc
     9721 aatcttttga ttcagcaaac catccaagta agtgaatttt tgcagcgtaa agatcaaaaa
     9781 gagtggctaa tatttttagc acaattagaa gaagaactca agagcagtca caatgtctct
     9841 gtgaaaaatc agcaactgca ctacacgatc gaggataaac agtatatcat cgaaagatat
     9901 caggcaatga tacgtaaaag aagaacttcc ggcggacatc aacctatgtt aacaagtatc
     9961 actgagcttc aattattgga aaaagaacac acaatttctt tttatgtaca ctttgaaaat
    10021 ggagaagatg gatatgccat atggacaaaa aacgatcagt agaaaatgtt ataagggctc
    10081 tattttattt tctgtcttgc ttttatttct tttatgtacg tttttattga tggcagcatt
    10141 agaaagctac cgtttgtcaa tggatcttaa tatacgaatc aaaaattatt atatagcaaa
    10201 aataatgaag cagatggctt tatcggaaca gtctacgctt gccgaaagtt cggaaggagt
    10261 tttttactac acaactggat ctgtaacata tcagtgggta caacagtccc tattcttaga
    10321 ggtggaagtc tcaccgttta tctttcggtt cacagaagaa gtaaaaaatg atacagatac
    10381 tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt tctgttataa cactgttttt
    10441 aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg aaaacgattg aaattttgtc
    10501 aattgttcga tagaattaaa caggtgaaaa aattgttata atatatctag aaatgaggtg
    10561 caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa aacctggaag ctattcagct
    10621 tttacagaat gcattgggaa cttccttttt agaagcctat gtcgaaaatg cagaaaattt
    10681 aatagatgat tatcaagtac gtgtggtgga tggagtgccg acaaaagaga caacgcaacg
    10741 gatcacagct ctttatgaag agttgaagaa actttctttc gagccagaag aatggcgcag
    10801 actgtctcaa ttacttttat tgaagggcag ccagacagaa catttgcagg cgaaccatca
    10861 actcactcct gatagtatcg gttttttatt tgtttttctt attgaacaac tatacacaaa
    10921 taaaaaagca ccggttaaga ttctggatat tgcggcagga atgggcaact tgttattaac
    10981 agttctttta aacctatcga atgcggggta ccaaacagaa ggaattggtg tggatattga
    11041 tgatacattg ttggctgtcg cagcttctac aagtgatttg acacaagcaa atgttcaata
    11101 ctttcatcaa gacgggttgc aggaattatt aatagatcct gtcgattttg caatcagcga
    11161 tttgccaatc ggttattatc cgaatgacga aaaagcaaaa gagtttttaa caagtaccga
    11221 agaaggccat agttatgcac atcatttatt gctagagcaa tcaatgaaat atgtgaagcc
    11281 ggatggtttt ggtttgtttt tgatgccgag tggattttta gaaacggatc aaagtgaaga
    11341 aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga atgattcaat tgccagatga
    11401 attgttccgt aataaacaat cgcaaaaaag tattttgatc ttacagaaaa aaggaccaca
    11461 agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct tcattaaaag aaccagagaa
    11521 ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca tctgctctgt aatcattcaa
    11581 cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat gctgggagtt caagtttgaa
    11641 atggcaatta tatcaaatgc cagaagaaac cgtagttgcg aaagggatcg tcgaacgtat
    11701 cggtttacaa gattctattt ttaccatcaa gtacggcgat aatcaaaaat tcgaacaaat
    11761 catggatatc gaaaaccacg aagtagcagt aaaaatgctg ttagatcagt tgattgaact
    11821 gcaaatttta agctcttatg atgaaatcac cggagtaggg caccgtgttg ttcacggagg
    11881 agaaacttat ggggattcag tagtgatcga tgagaaagta atggaccgca ttgctgaatt
    11941 agcagaattt gctcctttgc ataatcctgc aaacttaatg gggatcaaag cgtttaaaaa
    12001 gattttacct gacattttga gtgtggctgt attcgataca tctttccact caacaatgcc
    12061 aaagcaaaat tatttatata gcttaccggt gtcttattat gaagatttca aagtaagaaa
    12121 atatggcttc catggcacaa gtcatcgtta tgtgtcggaa cgtgcggcaa aattattgaa
    12181 caaaccgatc gaagaactaa aaatcatcac ttgccactta ggaaatggtg tatcgatcac
    12241 agcagtcgat ggaggtaaat ccatggatac ttctatggga ttcacaccat tagctggggt
    12301 gacaatgggt acacgttcag gtgatatcga tccagctgtt ctcccatatt taatggaaaa
    12361 attagagatt gatattgatg aaatgatcaa tgttttgaac aagaaatctg gtctgctagg
    12421 cctaacagga ttgtctagtg atatgcgtga tcttgaaaag aactatgaaa aagaacatat
    12481 tcgtttagct tatgacattt tcgtggaccg catccgaaaa tacatcggcg gttatgtgac
    12541 aacgatgaat ggtgttgatg caattgtctt tactgctggt atcggcgaga atgacggaca
    12601 tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt tgtgaaatcg atccagcgtt
    12661 gaatgaattg cgtggtgaag aagtagatat ctctacaaaa gaatcaaaag taagagtatt
    12721 agtgatccca acagatgagg aactaatgat tgcccgtgat gtggagcgtc tgcgcggcta
    12781 agccaaagca ttcgttagga taaaagatga aacgcatgca agagggaatt cttgcatgtg
    12841 ttttttttgc ggagaagacc gcaaaatggt aatttaactt caatttccct tcttttttac
    12901 aggatcttct tagggaattt tgcttggaaa agtggtcaaa agggaaaaga ctggcatttt
    12961 atttattgat tttcaggaaa atcccaatat tttattttca ttcatcttta taagggacat
    13021 tacatatgta aaaagaaaaa acatttttag atcagcctcg tgggctgtcg actttattct
    13081 tcgctttatg tttagctaga aaaccgatcc gaaacattca ttgatcgaat aataaaaact
    13141 tgattttcca cctgtacttt gcctgaatcg tgaaagcaca ggtatgaaaa tcaagttttt
    13201 tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg gctggcggct gatctgctcc
    13261 tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa agtagcggtc atggctgact
    13321 gctaggagcg ttccttgata gtcttgcagc atttcttcaa tttcttctcg agcatagctg
    13381 tcgaggtgat tggttggttc atccaaaatc aggacgttga ttttttctaa aaagagtttc
    13441 agcaacgcca aacggatttt ttcaccaccg gataaatctt tgatccgttt acccacatcg
    13501 ctttgataaa agccactttt ggctaattgc cgtcgagcga cttcttcgtt tccagtcaat
    13561 gtgatggcgt aagccagcaa acggctatcg ggatcggcaa actgcagctg ctgcggcaaa
    13621 taaccgattt gcaagctact acctaatttg atggtgccac cgtaaggctt ttcctcaccg
    13681 agtaataatt tgagtaaggt tgattttcct gcaccattgt cgccgatgat ggctaaatga
    13741 tctccacgat agactgcaaa actgctgtct ttgaatagta cttgatcagc gtactgctgg
    13801 tgcaagtttt gaatgatgaa aacttctttg ccagattttc caccattaga caagctttgc
    13861 agccgatttt tcgggggagg tggcggtttg acgagtgtca gtttcgccag gcggcgttcc
    13921 agttctttgg cttttttgaa gaaggactca ttgtctcctt catgtgccca ctgacgaaaa
    13981 cgacgaatca tgactttcaa ccgctggact tctttttgct gcagttcgta attttttgtc
    14041 agctcagcca agcgagcttc tttcaagact cgaaaacggc tgtagtttcc gggatactca
    14101 atcaatgcac cttcttccag ttcccatgtt tttgtggtga cttgatcgag aaattgccga
    14161 tcatgagaaa cgaccagaac cgattttttt gtattctgta aatagttttc caaccagcga
    14221 atactgttaa gatctaaatg attggtcggt tcatcgagta aaagtaaatc ggcatcggct
    14281 gctaaaattt tggctaaagc gactttaact cgttcgcctc cgctaagttc tgttaaccgt
    14341 gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg ctgcaatccg gtcttctaat
    14401 acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac cgtaacgggc cagacacttc
    14461 tctaagtttg tatctggttc tgtcatcaat gcttcgtagc gactcatttg ctgtttgagg
    14521 tcctgtatct cagcggcaga atggagcaag tagtggttga cggtcgcttc ggaaactgta
    14581 agttcttgtt cgaccgtccc gatctttaag ccttttttgc gactgataac accgctgtcg
    14641 acaccttctc tgccggtcaa gatttgtaaa aaggttgatt tgccagttcc gttgataccg
    14701 actaaggcaa ttttttcacc agcttcaatt tgacaggata aatcctcaaa taatggattc
    14761 ccgccgattg tttttttaat agaaattgct tgtatgatca tgatctctct ccttgagacc
    14821 actcaaaaag gccatggtta tccatggcct gtgttattta aactcagatt atcatgagaa
    14881 aaataccttg agatggccat tgattttacg tgaattcaac tgttttggac aaacttgtcc
    14941 cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc gatatggtat cgttggtaat
    15001 tttcattact tgttatttta gtcatgcgca tcatagtatt ttccctcctt cgtgtagttg
    15061 tcttttccag tgtacctgaa agtgtcagaa agttcaacta gcgctgacat ttacttaata
    15121 tttatcgatg tgtaattctg tcagagagct ttttttatct gactgagggt aatgtctcgt
    15181 ggaccacatc attgtattct gagctaaacc agcgttcagg cgaaatcata tggtgtttga
    15241 taaaaaactt tgtctacatg acttcctttt gccatttgct taataccgtt gcattaaaca
    15301 gctcttctct ttggaaatct caccaacatc gcgttcagga gtatcgttgt ggttgcaatg
    15361 gcaagcggta gcctttcatc aattcttgaa tgatttaccg aatgtagcac ttgccttctc
    15421 gcggtacccg aacccgaaat aaaattttag tttggcgata aaatcggtat ccgccatcga
    15481 tacttcatat tttttcgtat cagattcatc ggaaacttcc acattgacga accaataagc
    15541 tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac taatcgccct ggtgttcact
    15601 tttttttatt ttacttgaac tcattgactg ttttcccttt attttaaaca ttataaagac
    15661 cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt tttgtgcaaa aaaacctgcc
    15721 gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg tctttagctg gcaattggtt
    15781 ttctaaatca gttcggacaa caagcacatc gcaagctgcg tttctaataa catattcaga
    15841 aacagaacca ataaataaac gttctacagc attcaatcct gtggctccta acataatcaa
    15901 atctacttga ttatcttctg ggatttctct ggcaataatt tgttttggtg agccatattc
    15961 tacgacagag gttacattat tcaagccagc tttcttcgca ttgctttcat aatctgccaa
    16021 tgtttgcttt gccatttcag tagcttgttc tgcaagcatt ccatcaaagg atgaaacggt
    16081 ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt tctccgttat ttcggatagc
    16141 tacattgacc gcctttttaa aagctaattc agcttcatca gaaccatcta cggcaaccat
    16201 gatttttttg tattgttgta acataagcat tcactcctca agtcaatcgt attgtactct
    16261 tttattgtag aacactttag ggaaaaagag aattatttac tgctttggtt cagcaatttt
    16321 ttttgcaaat cggatactga aggtggcaga aacaataatt acgatcagta aaaagaccaa
    16381 ggcaataaag cgttgattga aaaaagctgt taaaataccc attacgccta aaacagcata
    16441 tgcagctccg taaaattgta ggaatccttg atttttatca tttttgttga tcaatacaaa
    16501 aaagatatcc tgtttattaa ataaataata gctaacgaat aataatacta aagtggcgat
    16561 aatcattaaa atcctaatca tgaacaaatc ctccaaaata agcaacaaat agaaaaagca
    16621 atttgccaag agcaaattgc tttattcttt aaaagatggt aatctccgtt aatgccgttg
    16681 tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg gtcccatggt cgtagcttcg
    16741 aactaccaaa tgataaggca tgttaccagc agtgacacaa gacgggtccc aagatatcaa
    16801 taaaaatgtt cggtcaacac acaaagagga caattcgaac atcacagatt tcccacctct
    16861 atagtagcat atgaatttaa aaagagcaat gagagagctc gcagaaacag aaccgttttc
    16921 ttgtctagtt ttttggagga tgctttttcc actcttttat acggtaatac tgttgaccca
    16981 gagcctgttc atatttccct gtatcttttg gctgataata ctgagcattc ttcagtttat
    17041 ctggtaaata ttgctgattg acccaagcct gatcaaaatg atgaggatac tgataaccca
    17101 cgccacggtt gagtgatttc gcacctttgt aatggctatc tctcaaatga tcaggaacat
    17161 ctccagcttt gccctctcga atatcagcaa gtgctgcatc taatgccatg taggcagaat
    17221 tggattttgg cgacaagcaa agatcgacga cgacgtctgc tagtgggata cgtgcctcag
    17281 gcaatcccaa tttttcagca gctaatacag cgttcactgt acgagccgca gcagcaggat
    17341 tacctaaacc tatatcttca taaccgataa ccatcagacg ccggcaaata cttgccagat
    17401 cacctgcctc aactaaacgg gcgagataat gaagtgcagc atccacatca cttccacgaa
    17461 tcgatttttg aaaagccgaa ataacgtcat aatgagcatc tccatttttg tcatgggtaa
    17521 gagcttttct ttggacacat tcttcaatga tcgacagtgt aagatgaatc cttccttctt
    17581 tatctgaagg agtcgatagt gtagccagtt ccagcccatt caatgcactc cgcaaatcgc
    17641 cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc taactggatt gcttgttgtc
    17701 caaggccgcg ttctttatcc cttaaagcat gttcgactgc caactggatg tcttgttctg
    17761 ttaatggttt gacttcaaag atctgtgtac ggctgcggat cgctggattg atggtaatat
    17821 aaggattctc agtagtcgct ccgatcaaga tgattcggcc actttctaaa tgaggaagca
    17881 gaaaatcctg ttttgtttta tctaatcggt gtacttcatc caataagagg atcactgtac
    17941 cactcatttt tgcttcttcc gctacaactt gtagatcctt ttttgaatct gtagcggcgt
    18001 ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc acttgcgata ctcgtttttc
    18061 cagtaccagg aggtccatat agaatcatgg aagagagcat gcgagcatcg accatgcggc
    18121 gtatgatttt tccttccccg actagatgct gctgaccaac gacttcatcg atcgttcgag
    18181 gtctcatgcg ataagccaat ggctgttgca taggattttc ctccttcttg tttttacgtc
    18241 ccttaattat atcatattta gcaagatgcc tagaaagaac agccttctgt tgttttgttt
    18301 tcatagctta tccaactaat attgtgtatg ataaagtagg tgagaaaaat gacggataaa
    18361 caaaagatag atgaattttt ttggaataaa acgacgccag aagtggcacg ggatttattg
    18421 gggatgtatt tggagcatga cacaccagaa ggaagattag ctggatatat tgtagacgcg
    18481 gaagcatacc taggtccaga agacgaggcc gcccatagct atggtttaag aagaacaccg
    18541 cgtgtgcggg caatgtatga aaaaccaggt acgatctacc tttacacgat gcatactcat
    18601 cggattttga acatcattac tcagccagaa gggattcctc aaggagtaat gatacgtgca
    18661 atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc gtggcggaaa aactggtcca
    18721 gatatcagca acggtccggg aaaattagtg gaagcattag caattcctca agaactttat
    18781 ggtcagtcta tagcagatag ttcgttacgc ttagtttttg agaaaaaaaa gacaccaaaa
    18841 aagatcaatg ctttgcctag aatcggtatc ccaaacaaag gtgtatggac agaaaagccg
    18901 ttgcgttttg ttgtgtcggg aaatccttat atctctttac aaagaaaaaa ccaaatcgag
    18961 aaagattggg gttggaggaa agaaaatgaa aaagaaggat ctattaacat atttagatga
    19021 aataattgaa aaacaagtga cagactatga tgtagcttta gattgggata caaaaaatca
    19081 tacgatcgag atcgttgttc gcttgtttgc agaaaataaa gcacaatttg agattgatga
    19141 tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt gaagacggcg tacttctttt
    19201 taatcctcag aaatctgttt ttgatgaacg caattatcta gcagtaatcc cttatgaagg
    19261 aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg gaatatctaa atgaagtatt
    19321 agctcaaggg caaagcgatt tgcttgattt tttggatgaa gacgatgacg aagctgtatt
    19381 cgagcttcac tgggataatc agcaactagc agaattagta gaagtaaaac agcaaaatga
    19441 agcagatact tatttaccgt acccaagtta ttaaggagtg acagatatga aatggaatga
    19501 agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt gctaatattt tgatggaggc
    19561 aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt gaacattttc agtcggatca
    19621 atatggtgag attttagata aaaatgattt tacatcgctg aaagaaggcg cactagtcat
    19681 ggcttatttt ccggaaacga ctttcttacc ggaaatcttg ccttttatca aagatcgtat
    19741 cacacaatta cctgagtttg gtctagctat tggaaaaaat aaagtaacag ttagtgaagt
    19801 agaggaaagc aactgggcaa cagcctggaa gaaatactat catccggtgc gtattacgcg
    19861 aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca gaagatcctc tagaaaaaat
    19921 tatcacacta gatcctggta tggcatttgg cacaggcact catcccacga cttccttgac
    19981 attgacagca ctagaaacag tcttgcgtgg tggtgaaaca ttgctagatg tgggaactgg
    20041 gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca aaagatgtct atgcctttga
    20101 tttagacgaa gtagccgttc gttcggcgaa agaaaatatg gatatgaatg aaatagccaa
    20161 agatgtccat gtatcagcta acgatctttt gacaggaatc gagattgaaa gtgatgtaat
    20221 cgttgcgaac attttagcag atatcatctt attgatgatc ccagatgctt ggcgtttact
    20281 gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa gagaagaagc aaatggttct
    20341 ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt ttccagcaaa aagactggta
    20401 tgcaattatt ttgaaaaaac cagaggtgga atagatgcag cggtattttt taaaagaatc
    20461 atatgaatca aaagactatt ttgttttaga aggggaaaac tatcaccaca ttgtacgggt
    20521 tatgaggatg gaacccgagg atgaagtttt tttggcattc aatgatcaaa tagccattgt
    20581 tgcagaaatc acggagatta ctgaagaggc tgtttatctg aaagaaatta aaaaagaaga
    20641 aaatcaaaag gaactgccaa gttcgattac catagcgagt ggctatccta aaggggaaaa
    20701 gctggattgg atcgttcaga aatccactga attaggagcg caagcttttc ttggtttccc
    20761 ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt atgaaacgac aacaacggct
    20821 tgaaaagatt gctcaagagg cagcggaaca atcccatcgg cagatcgtgc catcagtaga
    20881 gttattggaa acgaaacaag atttatttga gcggctagat gattatgacg tggtcttggt
    20941 tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat gcagccaaaa ttcttagtga
    21001 aacaaaacca gatagtcgaa tcttagctgt ctttggacca gaaggtggat tatcccgcga
    21061 tgaagtcgat tattttgtag aacacaaagg gatactttgt ggattaggac caagaatcct
    21121 tcgtacagaa acagcacctc tttatttatt aagtgctgct agctatcaat gggaattaat
    21181 gaatccttct ttcagctaac gttcttcatt aataaaagag tatcgacaga aagaattgaa
    21241 aaaatagtca acaatttgta taatagaaac aattatttat acaatggata cgaaaaatcg
    21301 tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa acgtataact gcacggattt
    21361 tgggacaatt atcaaacaga tatttgtccc gattcccgtt ttaccgaaaa aagaaagggg
    21421 gatcgattcc tgtgacaaaa gaagaaattt taacgggacc cggtgtcata aagctcgtta
    21481 gtcaatacat gggtcccgaa catgtagcat ttgttgagaa agcatgcgaa tacgcgactg
    21541 ctgcacatga cggacagttt agaaaatcag gcgaacccta cattattcat cctatccaag
    21601 tcgcaggtat attagcagat ttaaaaatgg atccccatac agtggctaca ggcttcttac
    21661 atgatgttgt tgaagataca gaaatcactt tagaagatct gagagaagaa tttggcgatg
    21721 acgttgctat gttagtggac ggcgtaacca aattagggaa aataaaatat aaatcccacg
    21781 aagagcagct agcagaaaac caccgaaaga tgctgctagc aatggctcaa gatttacgag
    21841 ttatcatggt caaattagct gatagactac ataacatgcg tacgttgaag cacctgcgag
    21901 aagataagca aagaaggatc gctcaggaaa ctttagaaat ctatgcgcct cttgcacatc
    21961 gtctagggat cagccggatc aaatgggaat tagaagatac agcacttcgt tatctaaatc
    22021 caaaacagta ttaccgtatc gtccacttga tgcagacgaa gagagaagaa cgtgaaaaat
    22081 acgtaagtgg tactgttgaa gatattcgaa tagcgacaga agagctgggg atttttgcag
    22141 aaatctatgg acggccaaaa cacatttatt caatctatcg taaaatgaaa gatcagaaaa
    22201 agcagttcaa cgaaatttat gacttgctag ctattcgagt gatcgtagat tcaatcaagg
    22261 attgttatgc tgtattagga gcaatccaca caaaatggaa accaatgccc ggcagattta
    22321 aggattatat tgccatgcca aaagctaata tgtaccaatc tttgcatact accgtgatcg
    22381 gtccggcagg aaatccggta gaaattcaaa tccgaacaca ggaaatgcat gaaatcgctg
    22441 aattcggggt tgctgcacac tgggcctata aagaaggaaa aaatgaaaaa gtagaaccag
    22501 atggtatgac gaaacaatta agctggttcc atgagatact cgaacttcaa gacgaaagct
    22561 atgatgcttc tgaatttatg gaaggcgtaa aaggagatat ctttagtgat aaagtctacg
    22621 tcttcacacc aaaaggagac gttactgagt taccaaaagg atccggacca ttggactttg
    22681 catacagtat ccatacagat atcggtaaca aaaccactgg tgcaaaagta aatggcaaaa
    22741 tggtgcagct tgattacaaa ttgaaaaacg gagatatcat tgagatcatg acttctccaa
    22801 attcatttgg cccaagtcgc gactggttga aattagttgc tactagcaaa gcaagaaata
    22861 agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa tgtgatcaaa ggccacgaat
    22921 ccgtggtcaa atgtattaca gatctaggat ttacgcctaa agatattttg acgaagaaca
    22981 aactgcaaga agcactcgat cgttttaatt atcaaacaga agatgatctc tatgcagctg
    23041 tagggtatgg agaagttagc cccttgacga tggccaatcg tctgactgaa aaagaacgta
    23101 aagaacaaaa aatcgagcag caaaagcaag aagcagaaga aatcatgaat cagccgaaaa
    23161 aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt tgtcattcaa ggggtagaaa
    23221 acttactaat tcgtatcagt cgctgctgta atccgattcc tggtgatgat atcgttggtt
    23281 atatcactaa aggcagaggg atatccattc atcgtcgaga ttgtccgaat gttcagcctg
    23341 acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg ggaagataca tcgaatacac
    23401 gaaaagagta tgatgcagat ttggaaattt acggctataa tcgttcaggc ttattgaatg
    23461 atgtacttca aacagtcaat gcgctaacga aaaatctcaa cagcgttgaa gcacggacga
    23521 ataaagataa aatggcgacg atccatttga cggttggtat ccagaattta tcccacctaa
    23581 agagtatcgt ggataaaatc aaagcagtac ctgatgtcta cagtgtacgc cggacgaatg
    23641 gataggagga cagatatgag agcagttgtt caacgggtaa gtaaagctgc tgtgtcagtc
    23701 gatcagcaag aaattggaaa aattggctac ggcctacttg ttttattagg cattcatgaa
    23761 accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa ttgcacaaat gcgtattttt
    23821 gaagatgaac aaggaaaaat gaatattagt gtagaagatg tgggaggaca aattttaagt
    23881 atctctcaat tcacactgtt tgcggatacg aaaaaaggaa atcgaccaag ctttgtggaa
    23941 gccgcacgac cagaaacagc tattccttta tacgaatcat tcaatgaagg aatcagaagt
    24001 cgcggcatca cagtcgctac cggacaattc ggagcagata tgtcggtttc tctcgtcaat
    24061 gatggacctg taacgatcat tattgattct aaaaacaaat aaagtaatct ggttttcact
    24121 agtaaaaact atgaaacacg tcgcttaagt tagactgata aactctaact taagtggctt
    24181 ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa aaaaagttaa gcttaaatta
    24241 tacaagttat tttttatagg aggttgctga agatgaaaga agtaccagta gtagatatca
    24301 aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa catgttggag gagttacgcc
    24361 atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa taaagaagct atcacgatct
    24421 acggatcatc agcagcaaaa gtattttatg atccaaggaa tttcaaacga aaaggtgcta
    24481 tgccaaaatt agtattgaag acactttttg gtcaaggtgg agtacagact ttggatggag
    24541 ctgctcatca ccatcggaaa aatattttta tggatctaat gacaccggaa agaatggaag
    24601 attatcatcg tatcttagat aagaatctga cacaagcact tgaagcacag catggtcaat
    24661 ttgaactatt tgatttaagt aagatggttt tctttacatc tatttgcgag tgggcaggta
    24721 tcaatttatc agcgatttct aaagatgagg tcgaaaaatt agcagaatat caaatctcta
    24781 tgatcagcgg aacgtttact tctccgattg atcacataaa aggtgtcgaa aatcgcaaaa
    24841 aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg acaaaatcct gtggctggaa
    24901 aagaaaatgt ggcattatat gcttttgcca atgccacaga tttagatggt cagcttttac
    24961 ctctggaagt ggcagctgtt gaattattga acatcatccg tccaacggtt gctctaactg
    25021 tgtgggcggc attgatggga catgcacttt tcagtagacc agatttgtat cagcaattaa
    25081 aaaatgactt ttcgacgtta caagatccgt ttattcaaga aatgagacgc tattatcctt
    25141 tcttcccaat gctacctgcg atttcgctga aagaagtaga agtagatggt tatcgtatac
    25201 cagaaggcag ttgggttatt ttagatttat atggaacaga ccatgatgaa cgaacagttg
    25261 aagcgccaga ttcctttatg atcaaacggt atgtagggaa agcaaaagat atttcctata
    25321 aagaagaata tgagatgatt gcacaaggcg ggggaaattt tcgacaaatg catcgctgtg
    25381 caggagaatg gatcactctt catagtctac gtgtgttttc cgatcagcta gtaaataagt
    25441 ttgagttctc tgtacctgaa caagattgga cgattccatt caatcagttt ccgacttatc
    25501 cgaatagccg agcgctactt tacaagaatt agattagcat aaacttatcg aaaaaaagga
    25561 agatcaaaca gtgctgatct tccttttttc atattactat aaattgataa ggtcaattga
    25621 tcaaaatcca cgaaatgctt catcaatggt ttgataatct tcattggata aatgcaaatc
    25681 taaagcttgg acattgtcag atacttgctc tggacgctta gctccaggta taactacaga
    25741 cactcgtgga ttcttcatgt accaagctaa aacaagttgc gcaggagtgg cttggtatct
    25801 tttagcaatg gggcgaagta catcgacagc ggttaaaatt tctctgaaac gttctccttg
    25861 gaaatctgga tttctttttc gaggatctcc ttcaccaaat tgtttttcct caccaagttc
    25921 atattttcct gtcagcaaac cggaagcaag aggaaaatag ggaacaaaac tgattttgtt
    25981 tttttcaaga taagggaata attctttttc agcctgccga tggatcaaac tgtatttatc
    26041 ttctactaca tcgacatatc catctgcatt cgcttctttc aattgttcta acgtgaagtt
    26101 tgacacaccg actgctcgga tttttcctgc ttcttttaat tcgtgcaatg tagcaactga
    26161 ttcatttttt ggtgtgcttt catccggaaa gtggatataa aaaatgtcga tgtaatcagt
    26221 ttgcagacgt tttagtgcat cttccaccga ttgtttgaga aactcaggag aattatcaaa
    26281 tgttcgccct ttatttggta catgagcagc ttttgtagcg atgacgacac gtgagcgatc
    26341 atattctttt tccttcagta cctcgccaat cagttcttcg gaacggccgt ttccgtaagc
    26401 ataagcggta tcgatcaaat tgatcccgct attcaaagct gtacgaacta cttgttttcc
    26461 tgtctcatct tccaaaccgt caaaaagatt atgtcctcca acggcatttg ctcctaatcc
    26521 aagctgttct gcgtagactt gtgaatgtcc gattcttatt tctgccatag ctgattcctc
    26581 ctttttgtct agtctaaaac aaaataccaa aaagctgaac taaaacgttt ttgtgagggg
    26641 aaatagtcag atatctcttg actttgagac tggtttttag taggttataa gtaatatcga
    26701 atgtcaatga aagagcgagt aagtttttga aactttacaa gagaggatca tcgaggctga
    26761 aaatgatcta aagtttcatt agcctaaaga cactctggag atggacgagt gaaaagccgt
    26821 cccgcgtacg agcgttattc gtaagaggaa agagaaatct ttcaaaatta ggtggtaccg
    26881 tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg tgattttttt atttacatag
    26941 ctaagaaagg gagagaaaac tatgagtttc caaagaccaa aaggaacaaa tgatattttg
    27001 ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag cacgtttatt attcagagat
    27061 tatcagtata atgaaatgcg tacaccgatt tttgaacact atgaagtgat atcccgaagt
    27121 gtaggggata caacggatat cgtttcaaaa gagatgtatg atttttatga caaaggggaa
    27181 cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg ttcgttcttt tgttgagcat
    27241 aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt tttatatggg accaatgttt
    27301 cgctacgaac gccctcaaaa aggacggtta cgtcaattcc atcaaatcgg agtagaagca
    27361 tttggatcag aaaaccctgc aactgatgtg gagagcatgg tgatggcact tgatttcttc
    27421 aaacagttgg ggatcacgca aatccggcta gtcatcaatt cattaggtga taaagaaacg
    27481 cgtcaagcct atcgtcaagc attgatcgac tatcttttac cttttgaatc agagctgagt
    27541 gaagactcta aacgccgcct tcatgaaaat ccattacgcg tattggacag taaagacaaa
    27601 cgagatcaaa aatttgtggc agaagctcca tctatcttag attatctaag cgagcctgct
    27661 caaaaacatt ttgataccgt tgtttccatg ctggatgcat tggctgttcc ttatgaaatc
    27721 gacagtaata tggttcgcgg actagattat tacacacata cgatttttga aatcatgagt
    27781 gacgcaccaa aaatgggggc acaagcaacg atttgcgcag gcggacgtta tgacaatcta
    27841 gtggaagaac taggtggtcc ttcaacacct ggcttcggat ttgcaatggg tatcgagcga
    27901 ttactcttga ctatggaggc agaggaagta gttatccctg catttaatga gttggatgct
    27961 tacgtggtcg cactcggaga cgaaacaaat atcgaagcat tgaaagtggt ccaagcgatt
    28021 cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc gtaaagcaaa agcgcaattt
    28081 aagacagcag ataaagcgaa tgccaaatta gtattggtga tcggtgaaga tgagcttgct
    28141 agtcaaacga ttaatgtcaa agcaatggca agccgaaaag aaaaaagttt tccattagaa
    28201 gagatatatg aacattttga tgaagtctat gatgaaatga ctttattcga gtaggaggaa
    28261 aaaaataaat ggcacaaaga acgatctatt gcggattagt ttccgaacaa caattagaac
    28321 aaacagttac attaaaagga tgggtacaaa aaagacgaga ccttggggga gtaatcttta
    28381 tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt taatcctgaa atcaacaaag
    28441 aagcatggga aatcgcggat aaatgtcgca gtgaatacgt gatcgaagtg actggggaag
    28501 ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc cactggtgaa ttcgaagtga
    28561 ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc gccatttcaa attgaagatg
    28621 atcaagcaat cagtgacgaa atacgtttga aatatcgtta tttggatctg cgtcgtccaa
    28681 aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa agcgattcgt cattatttag
    28741 atgataatga ctttctcgat atcgaaacac cttacctagg gaaatctaca ccagaaggcg
    28801 caagagacta cctagttcct tcacgtgtcc atgctggaca tttctatgcg ttgccgcaat
    28861 ctcctcaatt gtttaaacaa ttgctgatga atgctggatt cgaccgttat tatcaaatcg
    28921 ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca gcctgaattt acgcaagtag
    28981 atattgaaac aacgttctta acagcagaag aaatccaaac ttacacagag ggattgattg
    29041 cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt gccatttcct cgtatgacct
    29101 atgatgaagc aatggctcgc tacggcagcg acaaaccgga tacacgtttt gatatggaat
    29161 tgatcgatct aagcgataca gtcaaagaag tagaattcaa agtcttccaa atggctcttg
    29221 aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc tgcagatcgc tactcacgaa
    29281 aagacatgga tcagttaggt caatatgtag gacaattcgg agctaagggg cttgcatggc
    29341 tgaaagtaga agaagacgga ttgaaaggtc caattgctaa attcatggga gaagcaacag
    29401 aagcaatcat taaagcaaca gatgcaaaac caggtgactt gctgatgttt ggcgcagata
    29461 aaagtgaaat cgtcgctgct gcattaggtg ctatccggac acgtttagga aaagaattag
    29521 gactaattga tgaatcgaaa ttcaatttct tatgggtaac tgattggcca caatttgaat
    29581 tttcagaaga agaaggtcga tatgtctctg ctcaccatcc attcacgatg ccaaaagaag
    29641 aagatatccc gttattggct gaagaccctg caaaagtcta tgcagaagct tatgatatcg
    29701 tcttgaacgg ttatgaacta ggcggaggtt ctatccgtat tcatactcgt gagcttcaag
    29761 aaaaagtact agaaactcta ggctttacaa aagaacaaat ggaagaacaa tttggtttct
    29821 tgttaacagc tttagattac ggattccctc cacatggcgg tattgcatta ggattggacc
    29881 gttttgcaat gttattagcc ggagaagata atattcgtga agtcattgcc tttcctaaaa
    29941 acggaaaagc agcagatgtg atgagtgatg caccaagtat ggtttcgcct ttacaattgt
    30001 tcgaattgaa tattgatgta acgaatatag aagaatagcc gataaaaagg taaaaaggat
    30061 ttgtcgaata cgcttctttt gacaaccttg atttaattga aaaacacttt ataatgaagc
    30121 cataacatat aaagcggggc ttttccatga aaaaaacatt cgaaaattgg gcttatcatg
    30181 attacacgac caagctttcg gtatctatcg tttatgcgtt gatcgcatca gtcgcattga
    30241 atttctttta tcagccagga catatttatt caagtggtat tactggtcta gctcagattt
    30301 tgacgacttt gtcaaaatca ctgataggat tcgaagtacc ggtatcaaca actttgtatt
    30361 tactgaatat acctttgttt gtattggcgt ggttaaaaat cgggaagaag tttacaattt
    30421 tcacttttat cacagtcact ttgacctccc tttgtatcca tattttaccg gaaacagtcc
    30481 tatctaccga tccaatcatc tgtgcgttgt ttgggggggc tgtgatggga ctaggtattg
    30541 ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga tatcgtgagt atcacgattc
    30601 gtaaaaaaac aggaaagtcg attggttcta tttcgatcta cttcaatgca ttgatcgttt
    30661 ttgttgccgg aattctattt ggctggcaat atatgtttta cagtacgttg tctattttcg
    30721 tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa gaagatgcag gtaatgatcg
    30781 taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa ctgtatgcgt agagggatca
    30841 cgatcatcaa tgaagcagaa ggtgcttata agcatgataa gcagacagta ttgatgacga
    30901 tcgttacacg atacgaactg cctctactta gagaagcaat gaaagcttct gatccacaag
    30961 cttttgtaag tattgcagaa aacgtccaga tacttggtcg tttctatgag gatggcattt
    31021 agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga cagacgttcg tttgtctgca
    31081 gtttttttct accatagtag aatcagcaaa gaaaaactgc ttttttattt gaaattttct
    31141 taaagttgaa aaaaagcatg tttatttttt taaatttatg ggatgataga aaaaaatagt
    31201 agagaaagga cacgattcta cttgctattt tcacttaata cccgtatatt acgaatgaaa
    31261 aaagtttatg aaaagttgga atattgaatg gaaaaacaaa aaaggttaaa aaacagccgc
    31321 tacatcactg ggtttgatgg gctccgttca ttagctgtgg tgggtgttat cttctatcac
    31381 cttctgccaa ctagtatgaa aggcggctat ttgggcgttc cgattttttt cgtcgtatct
    31441 ggttatttga tcacggatct gctgcggcag gaatgggaac aaaatggaaa aatcaatatt
    31501 tggcaatttt atgtcagaag aatgaagaga ctctatccgg cacttgtttt tatgcttgtg
    31561 acagcttcgg cttatatcac tttgtttcag cgagggctgt taaacaatct tcgaggagtt
    31621 gtggtcagca gcttactgta tgtcaacaac tggtggcaaa tcaataacgg tttatcttat
    31681 ttcgatcgat ttgctaatga gtcaccgttt acgcatatct ggtctttagc agtagaaggt
    31741 caaaactatt tggtttggcc gatcttgttt gtattgttga tggtatttgt tcgtaaaaga
    31801 aaatggatct tttatactgt gttaggtaca tccttcctct ccgccatctt gatgatgatc
    31861 ttgtatacac caggaggcga tccaacccgt gtttattacg gtaccgacac tcgtctgttt
    31921 tctatatggt tgggaagtgc gcttgcgttt gtttggccaa gtacgcgttt aaagaaaaac
    31981 atcccgaaac aagcgaaaaa tattctgaat atcgctggag gaatctcgct tttggcactg
    32041 attctttgct tcttcttttt agatgatcat tacaatttca tctattacgg aggaatgctc
    32101 ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag cacaccccgg agcaagtttg
    32161 aatcgatggc tgacaaatcc agtatttact tggattggca aacgaagcta tggcatttat
    32221 ctgtatcagt ttccagttat gattttttat gaagcaaagg tgactgatat tgccgatcat
    32281 gttttgttgc atactttagt ggaaatcaca ttgattctaa tcatcagtga actttcttat
    32341 cgatatgtgg agcgtccgct agcacgtttt gattatggtc gtacattcca agtgatgaaa
    32401 ggatggttca gcaaaccgat catcagtaag agcaaaccat gggttgttcc aggcacatta
    32461 cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga aaaacgctgt aacagccgat
    32521 caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat tagcagaaga aaccagaaac
    32581 aattcagaaa acaccactag ttcggttgac caagcgatcc ttgataagta cgagttgtcc
    32641 aaggcacaag ggaataaggc acaaaaatta gaactaacgg catttggtga ttcagttatg
    32701 ttggatgctg caacggatct acaagaactt tatccaaaag tagtggtaga tggagatgtc
    32761 ggacgacaat tatatgccag tgtaccgtac attgaaaaat taaaggaaca agatttgttg
    32821 aaagacactg ttctgatcgg actaggaacg aatggctcat tctcggaagc tcagtttgat
    32881 agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg tcaatgtccg agtaccgacg
    32941 aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga tgacgaaaaa atacgacaat
    33001 atgacgctga ttgattggta cagctacagt aatgaccata gtgactggtt ttatgatgat
    33061 caagtccatc caaatcctga agggatgaca cattacattc acttagtcag cgaagccttg
    33121 ctaggaggaa gttcaagtac gaacagcagt gagacaaacg tgacagaatg atagctacaa
    33181 aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt tttatattta ccgtgttttt
    33241 aacattcata tagattcagg aagcaaaacg gttgagtgtg tctgtaaata acgttctttt
    33301 tgttttcggg tgagtttttt gaatgcagct tctgcttttg ttgcttcgct tcttgaagaa
    33361 aatacttctg catggatcat cttaacaggt cttctttttt gaagacgagt atatttagca
    33421 ccagtaccat tgttatgttc ctctaatcgt ctagaaagat cggttgtata tccaccataa
    33481 aatgtctgat ctttacagaa taatacgtaa aaataatgtt catttgccat agagcatctc
    33541 acgaatctct gcagtgtatt ctccatccat attgtaagtg atcaatggtg gagcgacttt
    33601 gaatccgtct aatttgcctt ctttgattcc ttcaactaac aaaatatttg cctcttttcc
    33661 aggttttggg taaacaaatt ggatcctttt aggtgcaatt cgatagtctt ccatgacctg
    33721 aatgatttct aagaaccgat caggacgatg gaccatagta aagcgtccat ttgtttttag
    33781 tagcttactt gaaatatcga tcacttcttc taacgtcgta tgaatctcat gacgggcgat
    33841 tgccaggtgt ttattagggt tcgttttatt tgttggcagc cctttgaaat aaggtggatt
    33901 acagacgact aagtcacaag aattatgtcg aacgacagat aagctgtctt tcagatcgat
    33961 ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg atactacgtc ttgccatgtc
    34021 tgctagacga ggttgaagtt cgatagcgtc gatagccgcc tgggttcgtt tacttaaaaa
    34081 aagtccaact gcgccatttc ctgcacaaag gtcaacaatc ttcccttttt taggtacacg
    34141 ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa aacactttgg aactttggat
    34201 aatcttgata tcttctgcgt ataattggtc gattcgttcg tcttcgtgaa gcatggattc
    34261 cctccaaaat gaaaaaaata gtcgtctgta ttatactatt tttattaaaa agaaccaagg
    34321 tggcttgtca aacctctatt ttttatgcat actatgacca taagaaggga aaggaagaga
    34381 ctatgtttta tcgatttatg cgcggggtag ttcgagtact attagcctta gttaatggaa
    34441 atgcccgcta tgagaataaa gaagttttac cgcaagatga gaattacata ctagttgctc
    34501 cccatcgtac ttggtgggac ccattatatc tggcggtagc tgcagcaccc aaggaattca
    34561 gctttatggc aaaagaagaa ctgttcaaaa atccagttct gcgatttata ttaactcgat
    34621 ccaatgcttt tccagttaaa agagaaaatc caggaccaag cgtcattaaa acacctgtaa
    34681 agattttgaa atcgacaaat ttgagtttga tcatgtttcc cagtgggaca agacatgcta
    34741 cagaacttaa aggcggaatg gcattgattt ccagaatgtc taaagcgaaa atcgtgccag
    34801 ctgtatatca aggtccgttg acacttgggg atctgttcaa gcgtcagaga gtgacagtgc
    34861 gtttcggcga accaatcgac ttatcagata tcaagaaaac ggataaagaa ggtatggcgg
    34921 agatcgaacg acgaacacaa gcagcttttg acaaattgga caaagaagtg aaccctgatt
    34981 tcaaatatga agtcaaaaaa aaataaaatg ataagtttat gcgtgggcgg tcttttgaat
    35041 ctactgtcct gttcagttca gactttttca atgaaaacga gggagagacg tcaagtctgc
    35101 cctctttttg cgttccaata tgtaaaatag aagacaggtg tcatgtaatg gatcaaagtg
    35161 ttataataat tagaaggaca ttttttgagg tgattgaatg cgttttttac atacagccga
    35221 ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt gaagaccagc gtcatgtact
    35281 atcttctata ctagatatcg cgaaagaaga aaaagtagag gcaatcgtga ttgccggaga
    35341 cttatatgat aggagtgtcc cagctgttga tgccgtagaa ttatacaatg aattgatggt
    35401 cgattggaat ttaaaggaaa agatcccgat ttttgctata tcaggaaatc acgatagcag
    35461 tacgagactt tcagcgggta cgccatggtt cagttattcg aactattttt tgcgaacaca
    35521 gctagcagaa gcatttgaac cagtcgagtt taaaaacgta caatttttct ttcttcctta
    35581 ctttgaacca attgcagcga gattatattt tgaagatgac aatatccgaa cgatcaagca
    35641 agcggtagcc aaggtgatag ataaaatgca ggagctattt gattcagaaa aaaaacaagt
    35701 attgattagc catttttttg taatgggaag tgtaaaatct gattcggaaa cgaaaatcga
    35761 agtaggcggg ttggattcga tccctggtga actacttaca ccgtttgatt atgtagcact
    35821 aggacatttg cataatcaag ctgctttgag acaggataat gctcgctata gcggttcccc
    35881 attgaaattc tctttatctg agatcaacca aaaaaaaggc gtatggatcg ttgacctgac
    35941 aacaggcctg gagttgaatt ttagggagat cgaacccctg catgatattg aagaaataac
    36001 agcgagtttc aaagaattgc tggatcctgc cttttatcag tccgtcaatc gggagaatta
    36061 catctatatc tatctcactg atagaacgat catcccgaat atgatgaacc agcttcggca
    36121 aatatatcct cgtattttgg ggattgaacg ggcaaacggt cgagcagaac aaacaaagcg
    36181 caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta gcagaagcat tttttagtga
    36241 aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg ctcggagaaa cgttgcaaga
    36301 aatcatggaa atggaatgat caagaatgaa accgaaaaaa ttacaactta aaaattttgg
    36361 tccttttatc aatgagaccg tagatttttc acgtttgaca gaagcaccgc tttttttgat
    36421 cagcgggaag acaggagctg gcaaaacgac gatttttgat gggatcacct tcgcactttt
    36481 tggtgaaact tccggaagac ttcgctctgg aaaagagatg cgttctttgt ttgctacacc
    36541 agacgaagaa acaagcgtga ctttttcttt tgaacatcag cagatgagct atgaaatcga
    36601 acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac ggaacaaaaa aacaggcagc
    36661 caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa ataaaacaaa tcacaaaacg
    36721 tacagaagca gatcagttga tcagagaact gatgaatctc gatgcgaaac agttttctca
    36781 aatcgtttta ctaccccaag gagaattccg taattttttg gtttcatcaa gcagtgataa
    36841 agagactgtt ttacgtcatt tattcggtac tcaatttttc caacagttca atgagctatt
    36901 aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat ttagagcaag agctgcatct
    36961 tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca gcagcagggt ttgaagccgt
    37021 tttagcccaa tggacaaacc atcaggcaat actcgaaaaa caaatcacgg aagaaaaaga
    37081 agctctaacg gaacttcagg aaaaacaaaa gcaactagaa aattcctact atcgttttga
    37141 aagcttgaaa aaaagttatg aagaaaaaac gatattaata aacaagcaaa aggaattagc
    37201 tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt gggtattatg aatttacgca
    37261 acgattagtc gaaccaatcc agcgcctaca agaaataaaa gttgaaaaaa tagaactgtc
    37321 acaaaaaagt agtgagcaaa caaagaaact agcgatgact acgcagaatt atgaagagtg
    37381 gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa atcaaagaag ctgctcaaaa
    37441 actgcaaaac aaacagatga tggtgccaat tgtcgaatcc ttacgtagta aaaaacaaga
    37501 cgcagaacaa ttaacaattt cattggataa agaagaattg aatgccgaga agctgtatca
    37561 aaatctcgct atctatcagg aaagaactcg aattcttcaa gaaaatttac tggatcagga
    37621 aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa tatctagatg aaaagcagca
    37681 agaaatagaa aaacaaaaag caaaagacac tgggtcgctc gcacagataa gagaagaact
    37741 caaaaaagca actgaagaaa taacaaaaaa tagtcagcta ttgaaagaac agacagaaca
    37801 agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt gctcgtcttc agcttttttt
    37861 gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa caccccatac aacaattaaa
    37921 acacgaacag ccaaataagg aagatatctt aaaagtagaa caacaactgg cagaagctga
    37981 gtctgctgta gaaagaactc agcaaatcat tgccaaaagt gaagcacatg aaaataatct
    38041 tcaacaacaa ttatcagatt tagaacaaaa aatcatggca acgaatatac aattggctga
    38101 acaagaagaa cacttgcaac aggctttgaa aaaggcattt cctgagataa cagaacaaaa
    38161 aacaaaaaca actcttgtag taatcgaaca atctttagat agacgaaaaa gcaagatggc
    38221 caatgcaaga atagagctgg aagaaataag acaacaagca gaaaagctcg aacaacaatt
    38281 agctgtctac aaacagcagc agacagaaaa aaataatcag ctccagcaac tgcaaggaga
    38341 aatcaaaagt ttagaacaac aattgcaaga tacggatagt aactgtttag aagaggagat
    38401 acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa ctagctgttg agcagaaagc
    38461 aggagtgcaa ttgttgcaag atcttcatac gcttcggaca cagcttgatc ttcaacatga
    38521 acaaatagaa aaaatagatg aaaaaataga aaaggaacaa gaaagaatag ctaaaaagtt
    38581 agaaagccag tcgtattttt caacaattga agaaatctgt gcatttgcac aacagcaagc
    38641 acaatataaa gaaaaccaac gtacagttac ttcttatcat gaagagcaat tagtgacagc
    38701 tgaccggctg cgtcaattag aaaatatcca gctaactgag acattcccag atacagaaga
    38761 gatcagccaa cagttagaag aaataagacg tatgatcgaa ataaagcaag aaaagatata
    38821 cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac gatgaatgtc tttcgattta
    38881 tcacacaagc cagacgaaat tagatgagct tacgcaatta cagcagcttt cgcaaacatt
    38941 caacggagaa aatcctaaga agactagttt ggaacggtat gtgcttcagg tttatcttca
    39001 agaagtgtta caagtagcaa atgatcatct ccaacgttta acgaaaaatc gttatcaatt
    39061 tgagctagcg gataccattg gcagttatcg agggaaaacc ggattagaaa tcaatatcta
    39121 tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg tctggcggtg agagttttat
    39181 tgcagcactt tctttagcat tagctcttgc cgatgtgatc cagacacaag ctggtggtgt
    39241 agcaatcgat gcattgttta ttgatgaagg atttgggtca ttagatgaag aagctttgga
    39301 aatggcaatg gaagctttag aaacgatcga aagtgaagga agaatgatcg gcatcattag
    39361 tcatgtaaga gaattgaaag aacgagtatt acaacaaata agaattgata cagaaggtag
    39421 cggacaaagc aaaatacgct atcaacttgg ataaaaaggg agattggtaa ctatgaaatg
    39481 gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac ttgcaagaag gccgagtgtt
    39541 atcggttgtc cctgaccaag aagaaaacgt gtggcatgca gaagtcttgg gcagtgaatt
    39601 gtatatggtt gatttggatg gtacagcaaa agagatagat tactgccaat gtccctattg
    39661 ggaggaacat aaatattgta agcatacagt tgctgtggaa ctttatttac ggtcgaaaaa
    39721 tctatctcgt atcatgaaaa aagaccagcc aatgctagaa ccggtaaaag caacaagtga
    39781 gggagaaatc attgcaaaag gtttttctcg tctaaaaaca caaaacaatc ctcaagcggt
    39841 gcaacctttg attattgaat accaagtaga aacgattgag actaatcagt atcacccaga
    39901 gctttctatt ttggcacttt acttgcgtat cggttcattg gcatcaccga aaaaaacata
    39961 tatcgtaaaa aacatctatg attttttaca agcttacaca gaaaaagaaa cttacactgt
    40021 taataaacag taccagtttc gtctagataa acgggcgttt caaaaagaag atcaaaaaat
    40081 actcacgcat cttgcaggaa gtgctcagac gcatcaatta ttaggtacga acggcttaca
    40141 agtcaaagga aaactagata aaagatatct gcttctccca gtagaacaag cccgcttcct
    40201 gttagagcag atgaatcaga ctacacgttt gcatctgcag ataaatgaac aaaacgatca
    40261 aggaatacaa ttttccgatc aaaacaaacc tttatcattc aaagtggaaa aatttggaga
    40321 agattatttg ctgcaagcat tgaatgattt tgattttttc tttatgcatt atcagtgggg
    40381 agttatccaa gggacatttt attctctgac gaaatatcaa caaacggttt atcaaacttg
    40441 gaaacaattg attcgccgat tggagaaacc agaagtagtg ttcaagaaaa aagaattacc
    40501 gtctttgttt aaagagatta tccctctgct gtcagagatc ggcgacgtag ctattgcccc
    40561 tgcagtagaa gaggaagtag cagatatccc cgtcgaattt attttctttt tccgaaaagc
    40621 aaaaggaaaa atccaagctc gagtcgattt cgtctatggc gaagtcatct attcgactga
    40681 cccgaagcat gaggtctcga cagaacaggc ttttcaggtg ttacgtgaca cagtagaaga
    40741 gaaacgagta cttgatctat tcagaatgta tcgctaccag aaaaatgaaa ctggttatga
    40801 acgtccattg ccaagcgggg aagaactata tgcctttttc cgcacagaac tatctgtctt
    40861 ccgtcagttt ggtgaagtca gacttggaag aaaattgcga gagttgtatt tagatgcgca
    40921 taaacatcaa ccgaaaatcg aagtaaccga gaccggttca tggctcgata ttcgattcga
    40981 tgtgacaggg atagaggagc aagaaatcga tcatgtcctg caaagcttgc tgagaaatga
    41041 tgctttttat acattagaaa atggtcaggt tctttctttt gattcagaag aatttcaaca
    41101 aacaagccaa gtgttgcaac aattacggga atcgatacgt acagaagaag gaacgatcca
    41161 tgtacctaaa aatcaaggat tgatcattca aaatcaattg gaaaaaagca atgctacttt
    41221 tagtgaatcg ttccaaacga tggtacaaga tctcattcat ccagaaagat accaggcaca
    41281 gcttcccaaa ggattgaatg caacgatgag agattatcaa aagcaaggtt ttcgatggtt
    41341 gaagatgtta ggtcattatc agtttggcgg tattttagcc gatgaaatgg gacttgggaa
    41401 aacactacag acaattgcct ttttactttc ggaaaaagaa gaacggaaaa gcttctctgc
    41461 tttgatcgta gcgcctgcga gcttaattta caactggcag gcagaggtga gaaaattcgc
    41521 cccttcttta agtatccaag taatcaatgg gaataaaaaa gaacgtgaag aattactggc
    41581 taaagacaca gatatccgag tgacttctta tgccagctta agacaggatc tagctaatta
    41641 tcagtcgcaa aaaattgatt atttgattct tgatgaggca caaatggtca aaaatagcag
    41701 tacgaaaact gcacaagcat tgagagaatt agctgtacct cagcggtttg cactgagtgg
    41761 gacaccaatc gagaataatt tagaagaatt gtggtcattg tttgcaacga ttatgccagg
    41821 atttttcccg actaaaacaa aattcaggga acttgccgca gaagaaattg cgcaaatgat
    41881 tcgaccgttt attttacggc gagataaaca aactgtgttg aaagacttgc cggaaaaaac
    41941 agagatgaat ctatattctg cacttacaga agaacaaaaa actgtttatt tagcttactt
    42001 gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa gcattcaaga aaaaccggat
    42061 cgggatactt gctggtctga caagactgcg acaaatttgc tgcgatcctc gtctgtttat
    42121 tgaagattac caaggcggtt ccggcaagct cgaacaagta aaagatctac tggtagctgc
    42181 caaagagaac aaacgaagag ttttgctgtt ttctcaattt acagggatgc tgactatctt
    42241 gcaagaagag ttagccgagc tagggatctc gacattttat ttaagaggca gtacgaaacc
    42301 acaagatcgg ttaagtatgg tagacgcatt taatgctggc gaaaaagatg tttttctgat
    42361 ttccttaaag gctgggggaa ctggattgaa tctcacggga gcagatacgg tcatcttgta
    42421 tgacctttgg tggaatcctg ccattgaaga acaagctgct ggacgtgcac atcggatcgg
    42481 acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa ggaacgattg aggaacgcat
    42541 ggattcactc cagcaagaaa aacgcgaact gttccaaaaa gtgatccaag gtaatgaaga
    42601 acagttgacg aaactgactg aagaagacat ccgcatgatt ttaagtgtag gagaaatcga
    42661 ggagtaagag actcgaaaaa gcgttcagta atgggtaaga aagcctttct aaagaagcga
    42721 ttgtttattt gatttaaaaa atagcaagga ggctgggatt ccattttatc ccagcttcct
    42781 tgctatcatt tttttatttc tatttttaaa ggttatacaa agattgtctt cattgtctct
    42841 aacagctgat caaaagaaga aatctcgata tcgcaaattg gagtctgttc aattttgcgt
    42901 tctcgatggt tgaaccaaag cgcttgccag ttagcacttt ttgcacctag aacatcattg
    42961 tcgtaattat cgccaacgta taaggtctct tctggaagca tatgaaattc tttctcagct
    43021 agctgaaaaa tctctttttc tggtttttgg aaacctgttg cttgagaaat gagcatattg
    43081 cgggaaggaa tccaattgtt caattgaagt tgcttcagtt tttttgtctg atggtcagtc
    43141 ggcccatttg tgataattcc taatgggaca ttcttttcct tcaagaaatc gagggttttt
    43201 ttgacctcct catgcaagca gatattatca agttcttcct cgtaaatttt ttgaaataag
    43261 aggccatctt cttccgtaat atgagggtag tctaaatctt ttagtgattg gctgatccga
    43321 tgtgctcgca tatattctag tgtccaatct cctgccatta cttttgggaa gttttcatca
    43381 ctatgatggc ggaaccggat atatagtgga tgcatatctg catctgaaac aagcggaacg
    43441 actcttttga ccgcgtttcg aaatggctgc tgctgatcat acatcgtatc atcaacatca
    43501 aacacaactg atttaatcaa aattttacac gcctttcggt tttcattgat tttttcacaa
    43561 atccttgact attgtaacag aaaaaattgt ctaaaaaagc tgataaataa aggtttttct
    43621 tactatattt ctatttcctt gacagaataa ggtttttata tttcagtttt tttcactaaa
    43681 aatcgtgaaa cacgtcaata agtttgatga acaaattaat cattatggta gaagtcgatt
    43741 gatttatttt aatgcaatct ctgtggaatt ttcgaaagat ttattttttt aatacagtct
    43801 caatataact attcaaacat aaaataacgt tatttgattt atttgtttat agtaaatttt
    43861 atttagtaat cattgcttta aaatactacg cttatataac agagaaaata agcgttataa
    43921 taaagaacag tattatttaa aatgacagaa aacattacta ttaaagaaat aaaaaacata
    43981 aaaaatctta agaaatcggt ttcatttagc ttttgagata aaataatagt acagttttat
    44041 acgctgtttc atatgtttag aaaatttgaa ttagatttga aaactctaat ttttaaatga
    44101 taaaaaaagc gagaatgctg gttttactgt atattaacct tagataactt tcattttttt
    44161 aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat tatgtataat agaaacagat
    44221 gggagattat tatgatcttc tgttacaaaa aagtgattct tatcacgaaa aggagtggtg
    44281 cacgttgtta acattatata cttccccaag ttgtacttcc tgcagaaagg ctcgtgcgtg
    44341 gttgcaagaa catcagattc catttgttga acgtaatatt ttttcagaac cattgaacag
    44401 ttctgaatta aaagcgattt tacaaatgac agaagacgga acagaagaaa tcatctcgac
    44461 acgttccaaa gtattccaaa aactgaacat ggatttagat gatctgccgt tacaagaatt
    44521 attggaactt gtgcaaaaca atcctggatt gcttcgccgt ccaatcatga ttgatgataa
    44581 acgtctgcaa gttggtttta atgaagatga aattcgtcgg ttcctaccgc gcgatgtacg
    44641 acaattagaa ttacgtcaag cacaattgat ggctggatta tagaaggata atacgaaact
    44701 tggaaaagac tattacaaag atagtttttc caagtttttt tgtttttaca aaaagtattc
    44761 ttgattagat gtataaaaag gtagtagaag atgatgattg cacgaaaaat agagagggaa
    44821 aattagctga aaagtcggct atatagtatt cgtctgccaa aaatgttata gttaaataag
    44881 atagcttgaa aggttgaaaa atccgctggt tttattaaat tcgcttaaca gcgagatttt
    44941 ttttctttac atttaagctg atttcgctac aatgaatcta tattaaacat tgtggcaaag
    45001 tgaggtgtag caaaatggaa atggaacata ttaatgaaaa tacgatccgt gtactaattg
    45061 gtcatgaaga tttagaggaa cggggcgttt catttttgga tttacttggc aatcacaaag
    45121 aaatcgaaaa tttcttttat agtattttgg aagaagttga tatcgaagag gagttccgag
    45181 gaagcgaagc tgtcactttc caagttttgc caaaaggaga cggattagaa ctgtttatca
    45241 gtaaaaatct tccggatgaa gaaatttctc aatcagaaga ttctactgtt gattcagaag
    45301 atgtgagtga ctttcttcgt cagcagatta ttggcagcga tccggaggaa gaattatctg
    45361 gaagcgccct agaagaacag gaaagacaag caacttttgc ttttgaagat tttgaacaag
    45421 tgatccaact tgcaacagat gtcgaactag agtctgcttg gactgatttg tatttattcg
    45481 aagagcgcta ttacctagtc gttcacttct ggatggaaaa tctcaaccaa gcggatgtag
    45541 agaatcagat tgctcgaatt ttggagtttg ctgaaaagag cgatcgtaca ccagaaacat
    45601 tgaatgaata tggacaatgt ctgatggcgc gtaatgctat cgagcgaacg aaattttatt
    45661 ttgtataaat cagcattata gtaagagact gtgacaaaaa ttgtgtcgca gtcttttttt
    45721 cgtataaatg tgttcaaaag cttgcatctg cggtaacaag cccaaccaaa cgaaaaatat
    45781 tgggaactat ttttgtttag ctgttcttat tacttgatgc agaatagctt tttcacgccc
    45841 tcttttttcg tgtaaataaa ttcttagacg taaataagtt cagagggaga aaaatgctgc
    45901 ttgcaatgaa tgaatataat aagtgctgtt ttgctaaaca tgcagaaaaa ggaaagaaat
    45961 attattgtcg tggctgtcga aaaagagtga ttttgaaaaa gggaaagaaa aaatgtgctc
    46021 attttgccca tcaaaaatca gataactgtt cggtctttag tgaaagagaa tcaaaagagc
    46081 acttacagtt aaaggaatgc ttcatggatt ggctgggaca gtcagtagaa ccagtatttt
    46141 tggaagccta tttgccgagg ttgcggcaaa ggccggatat tttgttggcg aatctagcaa
    46201 tagaaatcca atgttctaga ctgtctcacc agagatttat tgaaagaacc caaaattatt
    46261 tgaataacag ttaccaagta tggtggatac ttggtaattc ttttcttgga caatcgcaat
    46321 tctcattgat agaaaaaagt tgctgctact ataatagaaa aagaggagtg cattgctgga
    46381 aagctgatct caagaaacaa aagctttatt tgtatcatca tattacagag actgtatcag
    46441 ggcatatttc cttttgcagt agctgctgga ctttttctag tagggacctt aaagaaatat
    46501 tcactagtaa cgaaattaaa atgaaccaaa tgaaaaaaat agaaaaagtg agtgaagatg
    46561 ggaagaaatg gctgacacgc caacttattc acaagcaaaa gaatacagta tccatacaag
    46621 aacaatgcta cttgcgtcat aaacatcttt tgcatctatc atcgtggatc tatcaaaaaa
    46681 gtcggttttt cttttattta caagaacagg tttttcttta tcgaatgttg tacgaagaag
    46741 ccttggaaaa ccagaaagtt cctgatttca actcatggct ttgccaaata aaagaacata
    46801 aaagggaatg gctttttcct atgattgatg aagagatggt ctatcagcag ttttttaacg
    46861 aatgcataca cttgtcttct ttgaaaaaat agtaagtata aaaataggct aggaatcggt
    46921 tttctatgta aaacaagatt tagttatgct aaaattgatg gaagcagaca catgaatgag
    46981 tagaaaagtt agatgctgtg acatgtggat gttagactat gatctatttt gatagataga
    47041 gctaagggaa gaaaaggagt ggatttcatg gaagtaaagc agttgccaaa acgagaagaa
    47101 ttgcctgaaa atttaacttg ggacttgacc aagatctttt caagcgacca agagtttgat
    47161 gagaaatatt tggaattatc agaagagtta aaacaatctg aaaaacacaa aggaacactt
    47221 gatcaaggcg cttctcaatt tttaaatgcg attgaattcg tattgagggt ttatcgccaa
    47281 actgaagtca tttatgtata tgcgcacctt aaaaacgatc aagacactgg aaatacagat
    47341 taccaagcgc tttatgcaag agcaagcagt ctgttttcga aagttagtga agccgtttcc
    47401 tggtttgaac cagaaatatt gcaattgtca gatgaccaga tttggcaata tttcaaagaa
    47461 gaaccaaaat tggaagtcta tcgccattat atccagcaaa tagtagataa tcgagcccat
    47521 gtcttatctg ctgagcagga atctcttctt gctggagcag gtgaaatctt tgatgcttca
    47581 agtgatacat ttgctgtttt gaataatgca gatctagttt ttccaacgat tgaaggagaa
    47641 aatggtgaaa tagtccaatt atctcatggc gtgtatggtc agttgctaga aagcacggat
    47701 cgaagggtgc gcgaagcagc atttaagggg ttgtacagtg tttacgaaca atttagaaat
    47761 acatttgctt ctactttagg cacacatata aaaggacata attttaaagc gaaagtccgt
    47821 aattacagct ctgccagaga agcgtctttg agcaataatc atattcctga aagtgtatac
    47881 gatactttgg tagacgtggt aaacaagcat ttgcctttgt tacatcgata catggaatta
    47941 cggaaacgtt tattagaagt ggaaaaactg cacatgtatg atctttatac accggtctta
    48001 ggggaagctc caattacctt tacgtacgaa gaagcaaaag aaaaagcttt agaagcactg
    48061 aaaccaatgg gtgaagaata catggccatc gtagaaaaag cattctctga acgttggatc
    48121 gatgttgtcg aaaataaagg gaaacgaagc ggtgcttatt cttcgggaag ctatgacaca
    48181 aatccatata ttttattgaa ttggcatgat acgctggatc agctatttac gcttgtccac
    48241 gaaatgggac atagtgttca tagttatttc actcgttcga accagcctta tgtgtacggc
    48301 gactactcca tctttttagc agaaattgct tcgacaacga atgaaaatat cctaacggag
    48361 tatttattgg aaacagaaaa agaccctcgt gtacgggctt atgtactcaa ccattacttg
    48421 gatgggttta aaggaacagt tttccgccag acacaattcg ctgaattcga gcatttcatg
    48481 catacggaag atgaaaaagg tgtgccattg actagtgaat acctaagtga tagttatggt
    48541 aaattgaatg caaaatatta tggtccagca gtcgaagaag acccagaaat taaattcgaa
    48601 tggtcacgga taccgcattt ttattataat tactatgttt tccaatactc gactggcttt
    48661 tctgctgctt cagcactggc gaagaaaata ctaaaccagg aaccagaggc actagagaac
    48721 tacttggctt acctaaaagc aggtaacagc gattatcctg tggaagtaat gaaaaaagca
    48781 ggagttgata tgacacaggc tgcatatatt gaagatgcaa tgtcgatgtt tgaacaacgt
    48841 ctaaatgaat tagaagaatt gatcgatcgt ttataaggct ttctattttt gattttctcg
    48901 aatagaaacg aaaaaaatac gtcggctctc ttgtaaggga gtcgacgtat ttttt
//
LOCUS       DAFEHS010000014        48145 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFEHS010000014
VERSION     DAFEHS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.014"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..363)
                     /locus_tag="LP327_000842"
     CDS             complement(<1..363)
                     /locus_tag="LP327_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1724983.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYAVCIIKRDK"
     gene            complement(538..2673)
                     /locus_tag="LP327_000843"
     CDS             complement(538..2673)
                     /locus_tag="LP327_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL1724984.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2756..4411)
                     /locus_tag="LP327_000844"
     CDS             complement(2756..4411)
                     /locus_tag="LP327_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL1724985.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4413..4727)
                     /locus_tag="LP327_000845"
     CDS             complement(4413..4727)
                     /locus_tag="LP327_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL1724986.1"
                     /translation="MNLLFQSIKSITVPELKEKILENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4897..5157
                     /locus_tag="LP327_000846"
     CDS             4897..5157
                     /locus_tag="LP327_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBL1724987.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5171..5485
                     /locus_tag="LP327_000847"
     CDS             5171..5485
                     /locus_tag="LP327_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBL1724988.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5970..6524)
                     /locus_tag="LP327_000848"
     CDS             complement(5970..6524)
                     /locus_tag="LP327_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBL1724989.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6650..8578)
                     /locus_tag="LP327_000849"
     CDS             complement(6650..8578)
                     /locus_tag="LP327_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL1724990.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8884..9600)
                     /locus_tag="LP327_000850"
     CDS             complement(8884..9600)
                     /locus_tag="LP327_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL1724991.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9601..11424)
                     /gene="glpO"
                     /locus_tag="LP327_000851"
     CDS             complement(9601..11424)
                     /gene="glpO"
                     /locus_tag="LP327_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBL1724992.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11438..12934)
                     /gene="glpK"
                     /locus_tag="LP327_000852"
     CDS             complement(11438..12934)
                     /gene="glpK"
                     /locus_tag="LP327_000852"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBL1724993.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13070..14530)
                     /locus_tag="LP327_000853"
     CDS             complement(13070..14530)
                     /locus_tag="LP327_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1724994.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14520..15815)
                     /locus_tag="LP327_000854"
     CDS             complement(14520..15815)
                     /locus_tag="LP327_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL1724995.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16147..16824)
                     /locus_tag="LP327_000855"
     CDS             complement(16147..16824)
                     /locus_tag="LP327_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBL1724996.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16854..17945)
                     /locus_tag="LP327_000856"
     CDS             complement(16854..17945)
                     /locus_tag="LP327_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBL1724997.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18046..18480)
                     /locus_tag="LP327_000857"
     CDS             complement(18046..18480)
                     /locus_tag="LP327_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL1724998.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19064..20521)
                     /locus_tag="LP327_000858"
     CDS             complement(19064..20521)
                     /locus_tag="LP327_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL1724999.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20518..22413)
                     /locus_tag="LP327_000859"
     CDS             complement(20518..22413)
                     /locus_tag="LP327_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL1725000.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22471..24246)
                     /locus_tag="LP327_000860"
     CDS             complement(22471..24246)
                     /locus_tag="LP327_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL1725001.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24296..25792)
                     /locus_tag="LP327_000861"
     CDS             complement(24296..25792)
                     /locus_tag="LP327_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL1725002.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25986..27608)
                     /locus_tag="LP327_000862"
     CDS             complement(25986..27608)
                     /locus_tag="LP327_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL1725003.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28058..29371)
                     /locus_tag="LP327_000863"
     CDS             complement(28058..29371)
                     /locus_tag="LP327_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL1725004.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(29616..29840)
                     /locus_tag="LP327_000864"
     CDS             complement(29616..29840)
                     /locus_tag="LP327_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725005.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29860..30282)
                     /locus_tag="LP327_000865"
     CDS             complement(29860..30282)
                     /locus_tag="LP327_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBL1725006.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(30424..31134)
                     /locus_tag="LP327_000866"
     CDS             complement(30424..31134)
                     /locus_tag="LP327_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725007.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31152..31648)
                     /locus_tag="LP327_000867"
                     /pseudo
     CDS             complement(31152..31648)
                     /locus_tag="LP327_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(31685..32137)
                     /locus_tag="LP327_000868"
     CDS             complement(31685..32137)
                     /locus_tag="LP327_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL1725008.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32272..32877)
                     /locus_tag="LP327_000869"
     CDS             complement(32272..32877)
                     /locus_tag="LP327_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL1725009.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33327..33947)
                     /locus_tag="LP327_000870"
     CDS             complement(33327..33947)
                     /locus_tag="LP327_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL1725010.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33949..34818)
                     /locus_tag="LP327_000871"
     CDS             complement(33949..34818)
                     /locus_tag="LP327_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBL1725011.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34841..35248)
                     /locus_tag="LP327_000872"
     CDS             complement(34841..35248)
                     /locus_tag="LP327_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL1725012.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35267..36556)
                     /locus_tag="LP327_000873"
     CDS             complement(35267..36556)
                     /locus_tag="LP327_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL1725013.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(36583..36885)
                     /locus_tag="LP327_000874"
     CDS             complement(36583..36885)
                     /locus_tag="LP327_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1725014.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(36910..39003)
                     /locus_tag="LP327_000875"
     CDS             complement(36910..39003)
                     /locus_tag="LP327_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL1725015.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(39492..40220)
                     /locus_tag="LP327_000876"
     CDS             complement(39492..40220)
                     /locus_tag="LP327_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBL1725016.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(40366..42987)
                     /locus_tag="LP327_000877"
                     /pseudo
     CDS             complement(40366..42987)
                     /locus_tag="LP327_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(43101..44432)
                     /locus_tag="LP327_000878"
     CDS             complement(43101..44432)
                     /locus_tag="LP327_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1725017.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(44398..45876)
                     /locus_tag="LP327_000879"
     CDS             complement(44398..45876)
                     /locus_tag="LP327_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1725018.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(46181..48016)
                     /gene="tdc"
                     /locus_tag="LP327_000880"
     CDS             complement(46181..48016)
                     /gene="tdc"
                     /locus_tag="LP327_000880"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL1725019.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT        13753 a        10357 c         8038 g        15997 t
ORIGIN      
        1 tttgtctcgc ttaattatac aaacggcgta ttcaaaagat ttatttactt ttttgtatag
       61 atttcgattg ttccacctag tcaatacaaa tgaggtaatc aggaaactga aaaaaatcaa
      121 tacaaaaaag ccgattaaga aatacagcat tttcttttct gataaaaaag cgtacaagga
      181 aaaatccttg gctctttctg gtctcgttaa tgggaaaaag acccatataa aaggaagaac
      241 aacgatcatc catcctaaaa gagccactaa cgtttgccaa atctttaacc agtaattacc
      301 ttttgaaaaa tacttatctt tcaccaaaat tgatgcaact tcttctcttg ttactttttt
      361 catatcctca cctcacgttt ttttgttacg acgtaggagt ttaccataca tttcatttct
      421 tttcacataa agtgttccta aatttttcct aagaaaaaga acaaaacatt tctcatcagc
      481 aaatgaaaaa tattttgttc tttttatcag ttgttctttt tattttttat ctcttaatta
      541 tgttgctctt tccaatacga caatgctttt cattcctcta gatatcgccg tgtacatttg
      601 tctgcgtctt ctttgctcat ctccatcgtt ttcaaatgga tataagatta catgatcaaa
      661 ttccagtcct tttgctagac tcaaaggaat gatttggcag ttttctaatc catatgaatt
      721 ttttttaatc gattcttgta gcgtttgggt ttcctcttct gtttgggtga taactgccgc
      781 tttttctcct tttagagaaa ccaaaatctc agctagtatt ttcaacaatt cttcagagga
      841 tcgaaaagag cggtattcgg gttcttttcc ttgcggtcgg atcgatacga tgtcgatctt
      901 ctcttgatcc acagcatagg ttttgaaaag ctctgtgatt gctccgctag aacggtaact
      961 atttctaaga tcataccgat ggatcggcag atggtagtca tcgaaacaac gcataatatc
     1021 agcaaaagta gtggaagaat taaagattgc ctgattctcg tcacctacta aagtaaagtg
     1081 agtttttgga aataattcga tcaatagacc taattgtgcc agggtataat cttgtacttc
     1141 atcaatcaat agatagcgaa atgctggtac atttaccttt tcaacaagta aatgacgaat
     1201 agctaatata atgactgctt catcaacagt acgtgtgggt tgccaagcat aagctgagcc
     1261 atatcgtttt tcataaattc tttcaaacaa ctgatgctca tccacccatg ccatttcttc
     1321 gatttgtctg gttatttttc gatatttttt ttgtaaaagt ttccttgcat aagctacaat
     1381 actttgttcc gaatcatcag aaatcaattt accgaataat tccaactgcc tatcttctgt
     1441 caacgagagt acctgattct gcacagcagg actttttgct tgtttcaaaa gatgattctt
     1501 ccattcgctc atcagtgcct ttttcgttgc ttgaagccta tcgatcaatt taggatgagc
     1561 aggaactttc tcatatattt tttcgatcgt ttttttagaa aggactgttt ttccattttt
     1621 agtaagtcca cggataaaat tcggatgatc taaaaacaaa ggatctgatt gttttagatt
     1681 atcgataaac tttttgctac gcaaacgttc tgtctgctcc gatacatttt ctcccgtgat
     1741 acgtttgaaa taagcctctt caccttctat ttcctctgct gaaagctgtg aaaccagctg
     1801 tatgatggtt agattcagtg gatttctttc acctaatgat ggtaggacat tcgaaatata
     1861 gtcgataaac cgatgattag gtgatagaat cagcaagtca tcggatgtca tcgtatctcg
     1921 atataaatag agcagatacg caatacgctg catgatagca gaggttttac cacttcctgc
     1981 tacgccattt accaagatat tttgactttt cacatcccga atgattgtgt tttgttccga
     2041 ttggatcgta gcagtgatag ccttcatttc attggtctca tcttggccta agacttctaa
     2101 taatacgtca tcttgaatag ctacagacgt atcaaaaaac ttgaccaaac gatctctttc
     2161 caaaagaaat tgtcgtcttt ctttaataac cgtatcgatt ttattttgac gaaccgtata
     2221 agaagatgga ccaatcatgt tattgtaaaa aagttcggca attggtgaac gccaatcata
     2281 aacaagattt tcatctgcat catctgtgaa atttgctgta ccgatataaa aggattcttt
     2341 tggctcatta tccagaaagt ctacaacgac ttttccaaag tagggacttt tcaataaacg
     2401 ttcgatcttt gctagctgat tttctgcgtt tttctgtttg atattcatct gatcgatttc
     2461 tctatttttc atttcaatca tcgaaaaagt atccaggtta tctaagtaac tgtcaaaatt
     2521 caatcgaagc tcgccagcca tttctttcaa cgactggttt ccttctattt ttgccgtatc
     2581 catcgattcc cgcagttcgt tttttttatc aattagcttt tgatacacgt tcttcagata
     2641 tgcttcttct aactgttttt gtgtgttgac catacacatt ctcctctttt tttaaagtgg
     2701 tggaaccgat tagtttacct cttttaagag tcgaatgcaa gctttttaac gagtatcatt
     2761 tgttaggata tatcagttct tcaggtcgga cagtttgata taaagaaaat gcaccatcaa
     2821 ggttttttac gtggtagcca gactgcttca agattctttc cgctagatag ctacgtaatc
     2881 cgctatgaca gctgacgata tattcttgtt ggctgtctaa ttcatttaat cggtcccgaa
     2941 gttcattcaa agggatatgt ttggcttcct tgaatttgcc cttttccaat tcttctgctg
     3001 ttcgcacatc taataaaatt tttccttttg ctagttctgt cggcaattca tgccattgta
     3061 cggtttcact aagcccttct gcaatgttca ttgctgcata tcctagcatg tttactggat
     3121 ctttggctga gccaaatggc ggtgcatacg tcaattctaa ttccggtaaa tcgaagatcg
     3181 tcaaatgccc tttgattgct gttgctaaaa tatcgatccg tttatccaca ccttttgccc
     3241 caacaccttg tgcaccataa atctcgccag tctctggatg gaagatcaac ttcagtaaaa
     3301 tatctgttgc ttctggataa tagcttgcat gatcttttcc tgatacatga atgacttcaa
     3361 aagacagttc gttttctttg gctgttcttt cacttaaacc ggtggatgcg gcagtcattc
     3421 caaacgttcg tataattgct gttccaatac cgccttgatt tcttcgagaa attccggaaa
     3481 tcgtgtccgc aacttgtcta ccttgacgat tggctggtga agcaagagaa ataagtgcat
     3541 cttttccagt gatttgctgt tttacaacga tagcatcccc caccgcataa atatcaggaa
     3601 tattcgtttg ataacgttcg tctactagga tacccccgcg tatgcctaat tcgattccag
     3661 cggtcactgc tagctggtta tcaggacgaa caccaatgga taaaataact aaatcagcag
     3721 ggatggtacg cccatcatcc agtcgcaatc gtttcccgtg gtcttcaaat ccagcgacag
     3781 catgagaagt aatcacttct actccttgat gagacaattc tttttctata aaagctgcca
     3841 tttcttcatc cagtgttgga agaatatgtg gtgcttgttc gatcaccatg acttctaaac
     3901 ctcttctttt taggttttct gccatttcca atccgatgaa tcctgcacca acgatcacgg
     3961 cacgctttgg ctgtttttct aatgcatgca tcactttatc gatatctggt acatttctaa
     4021 tagaaaaaac attgtcggct tctgttatcc ctggaatcga tggaacaact ggttttgcac
     4081 caggagataa aaccaatgca tcgtactgtt caatgtgttt ttgaccctca tgtacgactt
     4141 ctaccgtttt gttttcggga aagatggctg tcacttcatg atttggacgc acatctaaac
     4201 aaaaacgagc ggataatgat tctggcgttt ggacaagaag attttcccgt tcactgattt
     4261 ctcccgaaag ataataaggc aagccgcaat ttgcaaaaga aacatacggt cctttttcaa
     4321 aaacaacgat ttctgcatct tccattaatc ggcgaagccg tgtagcagca gacattccac
     4381 ctgctacacc tccgacaata acgattttca tcttatttcc ctcctactgt tcgatcaaac
     4441 cattgattca tgccaccacg tacattcact acatcataac cagatttctt taattcttta
     4501 gctgcttgtt tactcctcat ccctgattga caaatcacat agacggtctt ttctttgtct
     4561 ccatcatatc tgtttatact ttgtaatgga acatttttag ctccttttat atgcccgccc
     4621 cggtattctg caggtgttct aacatcaagt aacgctggat tttccaaaat cttttccttt
     4681 aattctggga ctgttatcga ttttattgat tgaaatagca aattcatttt ctgcacctca
     4741 tttacatttt tataaatacc tcatggggta tattaaacag gagctttatt aatatgtcaa
     4801 ccagaatgac tcttcatttt tttcactact ttttatattt gcataaaata ccgataaggg
     4861 gtattatcta attataaaaa tagaggagaa tgtgttatgg agattgataa taaaaatatc
     4921 ctgaaccgtt tgaaacgtac agagggacaa attcgtggta tccaaaaaat gatcgaagac
     4981 gaaaaagaat gtatggatgt catcactcaa ttaagcgctg ttcgttctag tattgatcgc
     5041 gtaatgggca tgatcgttgc agataatctg aaaaattgct ttgagaaacc tgatagtaat
     5101 ccagaagaac aagcggctaa actagaacaa gcgatcaaga tgattattaa aaagtaaagg
     5161 aatgatttca atgaaagtcg ttttccatat tgatgaatta gaaaaatggt cagaaacagg
     5221 gaaaaacgta aaaaacctga taaaagcttc tccagaaacg acgattgtcg tatctgtaaa
     5281 tggcatcgcg attaccggtt atctagactc tgccaatgca gaattcttag atttgcaagg
     5341 agtcgttttt cacgcttgcg ccaatgctat gcgtgctaat catatttcag aaagttcttt
     5401 acctgaacaa gtaatcgttg taccagcagg tgtccttgat ttagttgaac tgcaatccca
     5461 aggttatgct tatatcaagc cttagtcagt agaggttgcg acagaagtga tcagcttcaa
     5521 gaaatttcgg cttatttttc gaagaagcta cttctggaaa accgtgaatc aaaggttgtg
     5581 aaaacgctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt
     5641 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt
     5701 tgtgaaatca gcggtttgac ctaagtatta gagaaaaaaa cgggaaaata gctcctcata
     5761 ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt taccaagagg
     5821 ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca acttctcctg
     5881 tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt tatcaaaaaa
     5941 aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt tcggtactcg
     6001 acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa aatgactctc
     6061 acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac atagatgaca
     6121 gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt agatagcccg
     6181 attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa caacagccaa
     6241 agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa tttatctgca
     6301 aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc aatgaccccg
     6361 tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata aaatgactgt
     6421 ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga taaaagattc
     6481 ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc gccctacctc
     6541 ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat gatttcactg
     6601 tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat cacttgctgt
     6661 tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact agtactttat
     6721 gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta taatcgataa
     6781 acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc accagacgaa
     6841 cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc gtatcttcac
     6901 ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc cgatcacctt
     6961 ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct gtcttaggaa
     7021 gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct gcttgatgga
     7081 tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt tctcctgttt
     7141 ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc atagcagccg
     7201 gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg gtcgtggcaa
     7261 tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt gcagcccctt
     7321 catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc ctttttatct
     7381 ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac gcattttcta
     7441 aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc tctgtccctt
     7501 cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg actgtcttca
     7561 acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga tagtctttac
     7621 cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg ccttgcttga
     7681 taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct cggctaaatt
     7741 ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa tcgatcactt
     7801 cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct tcactgattt
     7861 cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt ggttcaataa
     7921 aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta tttttatagc
     7981 tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct tcttggatca
     8041 tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt tctttttcga
     8101 ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca ctgactttat
     8161 tattttttat tttctggtca atcaactctt ctacatttat caaatcagga agatgtttgc
     8221 tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt gtgatcatcc
     8281 gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga atcaaccctt
     8341 ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga acatgctgac
     8401 tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag acagtttgta
     8461 ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca attgctcttg
     8521 cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg ttgtccatat
     8581 tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag agataaataa
     8641 aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac aaagatattg
     8701 ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat tggctattcg
     8761 acttactgat tagataccag ctacactcac aaaacatacc ccattcttct aggtcttatt
     8821 tattttccac ttaactatag caagacacat tttggttgtc aaactttagt atgttttata
     8881 gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca ttggacctgc
     8941 gattggtacc catgaatatg cccaatctga agttccttta ttagctattg gcaataccgc
     9001 atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg ttggccctcc
     9061 taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg ttccaggtgc
     9121 aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac caattaattc
     9181 tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg ttcctaaaat
     9241 cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa caagaatggc
     9301 ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat tccaactaat
     9361 tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg ggcccatgaa
     9421 tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga tgacaatcca
     9481 gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac catctccaag
     9541 caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat cactgttcat
     9601 ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt ctaaacgttt
     9661 agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg catcgatcac
     9721 aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg tacgacgaat
     9781 gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta accgagctga
     9841 ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa agatacgtcg
     9901 ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta aaccggctgc
     9961 tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat cgaagtttcc
    10021 acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt attcttcttt
    10081 taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat cggtgatttt
    10141 cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac ctctggaaat
    10201 cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt cttctacttc
    10261 agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt tattgaagct
    10321 cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat taccaatcaa
    10381 cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat ttgcttctgg
    10441 atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga ctttcggatc
    10501 cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt tttctcttgg
    10561 aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg gttgcggtac
    10621 aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca ttttaggaga
    10681 gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac tcgtattgat
    10741 cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag ctttgacgcc
    10801 aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta ctagataagc
    10861 cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat tgttgcggaa
    10921 atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt ctctttccaa
    10981 tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat ttgccagttt
    11041 gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg ctccttcgcc
    11101 ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa tactttgtac
    11161 gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct tcagataacg
    11221 aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa aatcctgcat
    11281 ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac cggcaccagt
    11341 gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt gaatgctatc
    11401 ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct tgggtagctt
    11461 ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc atttgaactt
    11521 caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc caataaccga
    11581 cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt tctgctcgtt
    11641 ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg tttgctgcac
    11701 cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc gtatcgatga
    11761 tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct tcttttgtcg
    11821 taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa ggagcaccta
    11881 gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt ttcgctgcgt
    11941 cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat tgaatcgaag
    12001 aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt atgccgtaac
    12061 caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta ttcatgacaa
    12121 taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc atttgtccga
    12181 ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt ccataaaaat
    12241 gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt aacatcactc
    12301 gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg atattgaaca
    12361 acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg gtcagtttcc
    12421 atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct cgttcttgcg
    12481 cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag gcatcgatca
    12541 ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag ttatctgcaa
    12601 ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt cctgtatctt
    12661 tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca atctgatttg
    12721 gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa ttccagattt
    12781 catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac tctttttgag
    12841 aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta gttgtacctt
    12901 ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt atatgcaaac
    12961 gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt caaaatttta
    13021 ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat caattctttt
    13081 cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt gacgcaaatt
    13141 ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc accaaatcat
    13201 attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc agttctctga
    13261 tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg attccaatca
    13321 ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac tggatcatca
    13381 ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc gctgtttcct
    13441 gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc tgttccaaaa
    13501 gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca ttgttgatct
    13561 gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga ggacgataat
    13621 gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac tttttgctcc
    13681 gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa acaaaaaagc
    13741 tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta agatgcatca
    13801 cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat ttcttttctt
    13861 tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc ccgagccagc
    13921 aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg tccaatccta
    13981 gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga tcttccggga
    14041 tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg tataattgac
    14101 cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg atctttcgaa
    14161 aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat acttgctgat
    14221 gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa aacaatagaa
    14281 ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca tttcgctgta
    14341 cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa tattgatcaa
    14401 acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct gttccccctt
    14461 gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct aataatgctt
    14521 caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac acggtattct
    14581 ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc ttttactgca
    14641 attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg tgctccaaga
    14701 atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt tattgctgta
    14761 agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct ctcttcttct
    14821 gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg acgataggtt
    14881 ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc tgtacggaca
    14941 gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt tacttggatc
    15001 gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc tacccagatc
    15061 gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg gcgagaatga
    15121 ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac aatagcggtt
    15181 tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc ttgatttttc
    15241 atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag aattgacttt
    15301 cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc tatttcgatt
    15361 cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt ttgctgaaga
    15421 gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc agaaatattt
    15481 cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa tttttttcct
    15541 cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt gtaaacatgg
    15601 attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt ttcactggta
    15661 acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt cgattgcttt
    15721 tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc cgcatgaatc
    15781 cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc ttataaggtg
    15841 ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt caaccttatg
    15901 atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag gtctgcttct
    15961 caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg tttcaaaaaa
    16021 tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa taggaaacag
    16081 aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata gacacatcat
    16141 ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag cagattgagg
    16201 attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag ctgctccaga
    16261 ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta cttcattcgt
    16321 tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc cagctaaaat
    16381 agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac aaacagctga
    16441 gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta ttgcttctgg
    16501 aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat catttgcatc
    16561 cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt catcttcata
    16621 ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag gtacatatcc
    16681 gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat cataaaaatg
    16741 agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact tttctacatt
    16801 tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt gcgttattga
    16861 taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt atatgcttct
    16921 tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc tgcccagtca
    16981 ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa tacctgctct
    17041 acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc ttgaatcgtg
    17101 ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc tattcgatca
    17161 tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg ttcaagaagc
    17221 cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga gaaacgatac
    17281 cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt tgttaataat
    17341 tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc tcgtctattc
    17401 gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa ctcgattcca
    17461 tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat tgtttcaacg
    17521 atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc tgcaactgca
    17581 cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg tccaccgtgg
    17641 aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag ctcacttaat
    17701 cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt tccgatatct
    17761 tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg tttataataa
    17821 gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt cataggcgac
    17881 agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg atcaaattgt
    17941 gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca tctatcatta
    18001 aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt ttttttgtaa
    18061 cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat ttcccaaaac
    18121 tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag caaattggcg
    18181 tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt tgacgtatcc
    18241 tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat agagcatttg
    18301 tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa taaaaccata
    18361 agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca acatgcgatc
    18421 tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg gcttttccat
    18481 ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt caaaaaacac
    18541 ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat cttcaaagaa
    18601 tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg ttgtgaaaaa
    18661 gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
    18721 aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag aggttgtgaa
    18781 aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttgt
    18841 cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga gaggtcgtga
    18901 gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc tcatgtttta
    18961 tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt cgtaaagaag
    19021 gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg atttcgccat
    19081 ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt ttctttgtgc
    19141 cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac atttgtgaag
    19201 tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa tctactccat
    19261 cttcgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg cggtaatcgt
    19321 cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt tcagctatga
    19381 acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa cccttaggat
    19441 caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct ccagttatta
    19501 agtttccttg gaccccttcc aaattttctg gatgagcact gactgcacta gacatgtagt
    19561 agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca cctacttcta
    19621 tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga taatatcctc
    19681 ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt ttttgaatat
    19741 catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa ccgacttgtg
    19801 aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata aactgatggt
    19861 gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt actcctacgc
    19921 taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa tattttactt
    19981 tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga ataagctttc
    20041 gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa tgggaaagag
    20101 aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca taaaaaaata
    20161 atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc catgcgatag
    20221 acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct ttaaagtgat
    20281 gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga tcctgcgccg
    20341 cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct ttaggtaaat
    20401 cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca tcccatgcac
    20461 cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga aattttttca
    20521 tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat gactagtaat
    20581 gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat catatccctc
    20641 tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta ctttttttcc
    20701 atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa taccgatatg
    20761 gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg ttggaaaaac
    20821 tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa tgatcgcaaa
    20881 cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata aatcaattat
    20941 ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt cgatttcatc
    21001 cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga aataactagc
    21061 aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac ttcttgtagc
    21121 aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag ctccacatat
    21181 acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag caggttcagt
    21241 gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt tcttattttt
    21301 cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag tcatacatgc
    21361 tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg gtacaagtcc
    21421 ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag caccagataa
    21481 aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac ttgcataatc
    21541 tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac aaatgaatgg
    21601 tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct caatataaga
    21661 aagaacccat acagctaaaa ttgctggaat aaccgtactt gtatatcttg ctagaatcac
    21721 gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa tcgtaggata
    21781 aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact tttttgcagc
    21841 agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg cattcaaaat
    21901 gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg cgagtactcc
    21961 tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag cggcgatcat
    22021 gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac tagatgcatc
    22081 atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg catttcctac
    22141 tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta gttgctctac
    22201 cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg ttgcacaatg
    22261 gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt tcgctaattg
    22321 tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc ttcttacaag
    22381 ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc taatagcgaa
    22441 cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca ctgcttgaaa
    22501 gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac gcttgatctt
    22561 cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg tttctacttg
    22621 gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa ctaggtcaga
    22681 actaggagag attagttgct gaatggaatc tatcttttta tggattttta tttgattttc
    22741 ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta tggtcagctt
    22801 ttcacgcaaa atctgataaa tctccgaaga ttcaaactca tctagaacac taggtagata
    22861 aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg catcatattc
    22921 cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc attctttgat
    22981 ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac taattggctg
    23041 gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt cggcaagata
    23101 caaaaccaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta ttgggaagaa
    23161 acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta caaaatgatt
    23221 catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg ttagcaaaaa
    23281 ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa tcgtttgctc
    23341 aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca tcaaataatt
    23401 taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag aatagggtag
    23461 ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg tatacctgtt
    23521 tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc gagaaaatcg
    23581 tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt ttaatgtctt
    23641 ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca accttgcgtg
    23701 ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat tttcttcagc
    23761 aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat tgcgctcgat
    23821 ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa agaaaatgtc
    23881 tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca caatagttga
    23941 agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta tgatttgctg
    24001 tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg actggataat
    24061 gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg ttttcttgtt
    24121 ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat ttcttaatac
    24181 tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc tctttgttat
    24241 aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt aaaaatcatt
    24301 tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt ttataatatt
    24361 caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt ctatccacat
    24421 aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata tcgattggtc
    24481 cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca atttcctgaa
    24541 gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat tcatctggtg
    24601 cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa cggtctgtca
    24661 attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa ggttcgggag
    24721 atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct acgcgcattt
    24781 ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa tctaccgtgt
    24841 tagcttgcaa gtactgttcg tctccatcta acattactgg tgcacagtct tcttccttcc
    24901 atatcctgta aatataagct ggataatgcc ctcggcacat gacatcggag aaaaaccatg
    24961 aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca tatgcataag
    25021 tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg attttcttta
    25081 ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga taaatctctt
    25141 tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta accaatccag
    25201 cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc caatatttca
    25261 ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag tcgattaatt
    25321 ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca taatgactaa
    25381 gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg tcataaaata
    25441 cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt gcccatgaga
    25501 tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc tcttcaaatc
    25561 gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct aaagcaatct
    25621 cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg atcccgataa
    25681 gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca cattgattag
    25741 cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc ataactatct
    25801 ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa acaagatatt
    25861 ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt tttaatattt
    25921 atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc atctaagcaa
    25981 acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac tttgccgtct
    26041 ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg tggaacagtg
    26101 aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc ttcatatatg
    26161 cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc tccccagacg
    26221 atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa aacagaatct
    26281 tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac tcgaagccat
    26341 ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg cgtcctagca
    26401 ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa tccagaagaa
    26461 atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat atcatcgatt
    26521 gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc tttcatcata
    26581 tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc atttccccaa
    26641 cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca tccttcatca
    26701 cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact caagggtttc
    26761 aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa ttggaagacc
    26821 atggataatt catttctttt aggattggaa taaagtttag cgatttcggt agttgctccc
    26881 catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt catttcctga
    26941 agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc tttgccaatc
    27001 atatcgatca cgtccatacg aaagccacca atacctttat cgatccagaa attcatcatt
    27061 tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg acgcttgcta
    27121 tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga accactgaat
    27181 attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat ataatagtcc
    27241 cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc atcagaagta
    27301 tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc ttcaatcagt
    27361 tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt actgatatca
    27421 tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc atcaatacct
    27481 aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat accatcttga
    27541 tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg ccaccatttt
    27601 tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat catttatttt
    27661 agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg ttatcggtaa
    27721 cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga aaggattgat
    27781 ttattcaggg aagaatgctg atttcacaac ttctttataa acggtgttca ctcagctgtc
    27841 cctcggtatt agctcaaaaa acggcaaaat ataagaaact atttttttcg tttttttctc
    27901 taatactcag gccagaacac tgatttcaca acctctttat aaacggtgtt cactcagctg
    27961 tcccttggta ttagctcaaa aaaaggcaaa atatgaggaa ctattttttc gtttttttct
    28021 ctaatactca ggccagaaca ctgatttcac aacctcttta tttcacattc aatatccaca
    28081 tgaatcccat gaatacaaag aacagaccat acatgatggg agatacttct tttcctcttt
    28141 tagctgctaa catggtaatt ggatagaaga taaaacctaa tgcaatccca tctgaaatac
    28201 tataagtcaa aggcatgccg atcaagatca agaatgaagg gatcgcaatc tctaagtttc
    28261 cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc taccataatc aatgctggtg
    28321 cagttacttg tgaagtaaca actgatagta atggagaaaa gaacaagcct aacaagaaga
    28381 aaatccctgt agtaatagct gtgatacctg aacgtcctcc aacggcgata ccagctgatg
    28441 attctacaaa tgcaccaaca ggagaagtac ctaataagga acccgcaagc atagctgttg
    28501 aatcagaagc taaagctttc cctacacgag gcatcttgtt gtctttcatg aaacctgctt
    28561 ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa agtcacaagc aagaatgtca
    28621 gcactacgac ccacatttga agagaattga tgtcccctac atgttttaat gcaactaaaa
    28681 atgttggttt caaacttggt gctgcggcaa caatttcaga aggcattgga atcaaacctg
    28741 tgaccagacc taaaatcgtt gtagctgcca tcccgatgaa gatgccacct ggtacacgcc
    28801 gaacaagcaa gatagcagtt acgactaatc caaaaatcgt caaccaagta gaaccaacgt
    28861 tgagtgatcc taatcctact aatgtagatt cattcgaaac gatgatccca ccttcgctca
    28921 gaccaaggaa agcaataaat agaccgatac cgccagaaat cgcatatttc aaatctgctg
    28981 ggatcgcatc aatgatcatt tcccgcagct tgaagattgt gatcaaaata aaaatcaaag
    29041 aagcaacgaa tacgccggct aatgctgttt cccatggaat ccccattcct aaacaaacag
    29101 aataagcaaa aaaagcattg atcccaagag caggtgccgt tccaattgga taacgggcta
    29161 atacccccat taaaatacag ccgatcgcac tggctaatgc agtcgcagta aacactgctc
    29221 cttcatccat tccagaagca cctaatacag ttggatttac aaataagata taagccattg
    29281 agataaatgt tgtaaatcct gccagtacct cacgttttac cgtggtgttc aatttttcta
    29341 tttcgaagta ggaaagtatt ttttccttca tcttccatcc ccctaaatcc gaacattata
    29401 aataaagtcc gttaaaattt cgatttatta ataaaacaac gtgttttatc atatactaaa
    29461 acaaaacata agtaaacaaa aaattcggat tttaagcaaa atccgaacgt tccatctgat
    29521 taaatccctt tatctcaaag ttcatgaaca ttcactttgg attacaagaa tttctttatt
    29581 gtgttaataa agtttcttca ttctagtctc aaaaatcaat agtgtgattt gattttacca
    29641 tttcgtttct gttcttcttt gatgaattct tttgtatcga caggattcag attatttagt
    29701 ccgccaaaac ctgtatcgac catttttccc cagccttttg tcttcttttt tcctgatttg
    29761 agattctttg ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa ataaccttct
    29821 aatcttccca ttagatccac tgtttctcct cctttttatt caatcagacg actcagcttt
    29881 tcctataaca aaataattgc cttcagggtc cgaaaaattg aaagtcaaga cgccattgtt
    29941 gtctgtgatt tctcctggtt cttccatgtt ctcgtagata gtttcaaatt catccgtaaa
    30001 aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa aacttctgca caaatacttt
    30061 tgggaatacg cctaattcta attcttttga gatgctgagt acaatactct taaattcttc
    30121 tggcaattcg attgtttcag aaatttctgc accaattttt tctacaaaga atcggcaaat
    30181 catctctacg tcattgacat aaagcatcac acgtgttttt tgtatcatcg tttgaccctt
    30241 ctttctattt gattttcttt cctgtctata aactgattcc attatatcac gaaagcgcaa
    30301 acaccttgtg tttgatacta ttcaagaaaa ataaagcaat agtttctagg attgctttcc
    30361 tccacaaact ttttttacaa aaaaaaaacg gcaatgaatg ccgctcttct tatttcgatt
    30421 cttttataat tctgcttctg attcatcttt ggttagtgtg tgtctaacac tagttacgcc
    30481 aaatgtttga tcgatttttt ccttcaacgc ttcgattgcc tgtgcgccac gtgccgcttg
    30541 ttcagcattg atggattcaa tgaccagcac cgctgcttgt gtttcttccg tcagcatcgt
    30601 tctttgggct tcacgccagt tgagacagcg acagatcgct ccttgttcat catagtagat
    30661 gatttcttcc ggaagtgccg gagcatcgga atctgcacct aaaggcagga atgattcgtc
    30721 accttgggct tttcctaaat gcagatttcc tgcgatcttt tcgatatctt ctccgccgca
    30781 aggtacgcca aaagacaagg aaatactatt atagatatct acaagcggat tgattgggaa
    30841 aaattcccgt tcttgatttg cacgtttcag taaagcttca atagaagaac gtgcaccttt
    30901 tttagtcttg aatgtggtaa atgcctgacg ccactgagca atgacttcgt tttggctgaa
    30961 tgtttcttct gtaaggtaat ttcttgcttc ttttgctgcc tcattcaata attgatgata
    31021 cgtttcttca ctttcttttg ttacgtggtt atcgatacca tttacggata agatttcgat
    31081 ggttgcttca ggaaatactt cccagaaatt ggtatccact attacttttt tcatctgttc
    31141 acacctcttg attagaataa ttgcttgatg aatagatagt ctgtttgggg atcatctccc
    31201 atataaaaaa catgttcgct gtatttgata aatccttttt tacgataaaa agcgagcgca
    31261 acatcgttgt gttcccaaac accgagccaa atggctgttt tttgcttttc ttttgctttt
    31321 tcaatagctg tatctagtaa aaatgtaccg ataccttgat gtttaaattt ttttcgtaca
    31381 tagattcgct cgatttctaa aaaatgatca gccatttcct cagattgact cgttcctgta
    31441 tttagtttca ggtaacccgc aggctcttga tcgatactca ctaggaaaaa atctgtttct
    31501 ttatctttta gctcttctgt aagtttttct agtgcataag cttgtgtcaa gtaatcagct
    31561 aaatgctccg gtaattatct tttccaaatg tatctgtaaa tgtttcgata ctaatttgtt
    31621 gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc gtatttattc acggcttctt
    31681 cctttcaata gatcctttta ttgccttttt tgacaaaatt ccaatcttcg ctaacattct
    31741 tgcatacttt atttaaaaga tattccaatt gttttgcttc ttcgtcagaa agaccattta
    31801 atgcgactgc attggagtag ttgttttctc gaatgatgaa aggatagatt tctgctcctt
    31861 ttttagtcgg atagagtttc ttgatttttt tattttcttt atcttccaaa cgttcgatca
    31921 tcccattgga ttctaatttc ttgattgctc gggcagtcgt tgtacgatct actttgatca
    31981 tttctgctag tttttcttgg atgattccag gattttcgca aatacgtact aaataaagat
    32041 actgtcctct tgttaaatcg acttctttga attcgatatt gctaatagag tctagcgctc
    32101 gggcaatcat tccgatgtct cgaaggattt cagccatttc atcccacctt tattttgttt
    32161 gtaagactag tttacctctt tttgttgcaa atgcaactat ttttaataaa aaaatcaagt
    32221 tcttttttaa taactagtaa agtatattct tttcttcttt tacgaaaaag ctcaatcaca
    32281 atagatctgg tttttgtttt ctaaaataag taactgtttc ttcgttgttc gcttttctga
    32341 tagctttggc aactcttcta tactaaaaaa tcccatgttg gaagtttcat tattctcaat
    32401 aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca agtttataat actgaaaaag
    32461 ctgtggaata tctttttgtt tattcgtatc aaaaactgct cgcaactgag tagtttctac
    32521 tgttaatcct gtttcttcat agacttcttt acgcacattt tcttcaggag acaatcctat
    32581 ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga ttagtaggac
    32641 cttgccatct tttttgatat aggcacgaac atctactttt ggtgttggat gtccttctcc
    32701 aatcgttgtg agcgctttta tttcttcgaa agtttctttt cctatttcac tgatcagttc
    32761 caatgaaatc gtccggatct cctcaaaacg ctctttatca aatacatctt ttccataata
    32821 tagaccagct tgagaaagtg cgagtagtct tttgtattga gccaacttct tgttcattct
    32881 cttcccttct tttttagcaa atgtatgact atgtaatcaa gtatagaaga ctggtttaat
    32941 tacggcaaat aaatataatg aaatctgcaa taaataaaag aatgttaaaa aactcgagaa
    33001 caaaagacat tcgacttctc tggggctgtg aataaatggc gttcaaaagc ttgcatctgc
    33061 ggtaataagc cccatcaaac gaaaaatatg acgagctatt cttgtctggc tgttcttatt
    33121 aattgaggca ggacagcttt ttcacaacct ccccactaaa cggtgttctc ctaccaactc
    33181 ctcggtcaca agtcacaata ttcatcaaac atgagaagct gtttctttga aattgctcct
    33241 tgtgactcgg agctgaacga caggctcacg acctctcggt aaacggtgtt caaaagccaa
    33301 tttttcgata ttaagccatt tctttgttac ttattgtcat tgatttcttt ttctaattta
    33361 tccaatagtg gagtgtaatc cacttgagta tagtctgtta catcaaattc atatagtgtt
    33421 cctaactgga atttgtcata ttttcggtcg gcgatgatct ttttgaatgc ttctttcgta
    33481 atatgattgg tccctttcgt tcgatcttcc aatacgtcac catagtgata atgatccatg
    33541 atatagctca aatgacgatc tgattcacga tcacgtttgt accgacgttg ccataacact
    33601 tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc cgtatctatc tgctaattct
    33661 tggagtcgtg ctttttgctt atcactaaaa ggatactcgg acacaatcac tttctttcct
    33721 gcttccatat acaaatcaag tgctttgtaa tagaaaggat aaactttcgt cctttcttgt
    33781 tccgcccgtt cttccaaatt gttaaacccg atggattctg catagtattc cttgatttca
    33841 tcgagcgtga tggcatacat atctggaaat cgttcccgta gtttattcat aagatatgtt
    33901 tttcctgtac caggactacc agccagaaga atgaaatact ttttcatctc atttatttcc
    33961 taagttaatt ggactttcca agttcataaa cagtccaatc ttatgcttga caatttcttt
    34021 tactgcttta tttgctggaa tcacattatg gcgaagagac ttgttgattt ctgtttcact
    34081 aaaggtttct ttagctactt tcgtccagtt gactaaaagc tccgttccaa cattgaattt
    34141 gcggatcccg ttattgatca attcaggata atcttcttct ttcacacctg ttcctccatg
    34201 gataactagg gggacatcaa ctactgcatc gatttctttc agcaagggaa tgtttacttc
    34261 tgtttttgat ttgaattgtc catgattcgt accgatagca atagctaatg catccacgcc
    34321 tgtagcttct acaaatttca ctgcgtcttc cggtcttgta tataccttgt cattttcagc
    34381 gacatggatt ccttcttctg taccgccgat tgttccaagt tctccttcta cggaaacacc
    34441 gtgcgcatgg gcatattcta ctactgcttt tgtcttcaag atattttctt tgaaaggtag
    34501 atgggacccg tcatacatga cagaagtata tccgctatcg attgcttctt tgatatcatc
    34561 aaagtctcgg gcgtggtcta agtgtaagac tgcatcaacc atttcttctt ctgcaatagc
    34621 tgtcattaca ttgactaaca catgcatgcc gatatattta gcggtatcga cacttgtttg
    34681 aataatgatt ggcgaaccca tttcttttgc tgcgcctagc atggcaggaa gcatttctag
    34741 gttatgcgta ttaaaagctc caactgtaaa attcaactct tctgctgttt ttgttacttc
    34801 tttcaatgtt gtatacatct tttattgcca ccttatcttt ttatttgttt cctttgatcg
    34861 ttacttgttt tgcaatttgt cgcatctcat ccattttgcc catgtagtaa tcgatccctt
    34921 gatcatcacc agcacgagca gcatctgcac gcttctggtt atagaggctc atcagttttt
    34981 tatacccatc tccgatcgtc tgtttatttt ccaagctctt ttctaaatat tggatggctt
    35041 gatcgttctc atgcaattca gaaaatttaa gtccaacttg ttcataaagt tccgctaatt
    35101 tatgcctatc ttgggtagtg gaaatctctt gtttcaattg tgtaatctct gattgaatct
    35161 tttcggtcgc ttctggcgat agggcttttt cttcttttgc cagaactttt ttcggttctt
    35221 tttctttttt cttaaaaaat ccaaacatca ttttccctac tttcaattaa aacgctttca
    35281 aaactgggct taagatccag gcatataaga gtgctgcggc tactgtatct acatcggttg
    35341 ctgtagcatt gatgaatccc atcgaattaa agatcgttac tagcaaagcg ggcaataatg
    35401 taataaagaa accatgagca ataccgccaa tgatcgctcc tcggcgtccg ccaactgcgt
    35461 tgccaaatat ccctgctgtc cctccggcaa agaaattggt tagcatacca ggtaaaatca
    35521 tcgctaagcc gaacatgggc aaagtaaaca tggcaataat cgtaccaatc gttgtcgtaa
    35581 tgaaacctaa aatgacggca tttgggctat aaggaaagaa aactggacaa tctaaggcag
    35641 ggatcgcatc aggaactaat ttcatagcta tccctcgaaa tgccggcacg atttcaccta
    35701 acagtagacg tacaccagct agcaatatat acacacccac tacgaattgg atggcttgta
    35761 aaaaggcaaa catgatatag ttttgatctc ctgataattc agaagcgaat ggttcgcctg
    35821 caaataaaac agtgatgata tatagcggga ccatcacgac catgacagac aaataagtat
    35881 cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc gatcgacttt ttattttcgc
    35941 ctttctcacc aaaaagctta gcaacaccgg cttcaaataa gtacccgatc gtacaaaaat
    36001 gacctaaagc aatatcattt gatccagtga ttttacgaat gatcggttgc gctactgccg
    36061 gcatagcaat ggcgaaacaa gctccgataa aaccaccgac caagatcaat acgatccctc
    36121 gcagaccggc aaaatagcca aagactgtcg tcatcgttgc catccataaa attgcttgac
    36181 cagttaaaaa aatgtacttc cattttgtga aacgagccaa aataatattg aatacaaaaa
    36241 tggctaagaa cgtcagtgca atatctctac ctaaacccaa atcattcatt gcttgatcat
    36301 tgatagattc aatagaaggg atgatccctt gcatatgaaa tccttcagta aagattttcc
    36361 cgaaataagt taaacttcca acgatgatgc tcgaaccggc actcaatact tgaaatccta
    36421 ggagtgtttt taacgtgcca gagatgactt gtcctgctga tttcttctgc agtagtagac
    36481 ccaacatagc aatcaatgca atggtgatcg atgcctgagt caaaatattt tcgatgataa
    36541 aattgataac gctcatgttt ttaccctctt ttctttttgt atttagataa ctcccttatc
    36601 ttgcagaact ggggtgattt tttcttcaat ttcatttttt gaaacaatat ttttcaaata
    36661 gatcatcgcc gttttaggat caactgcgaa cttttcgaac tgcgaacgaa aattttctgc
    36721 tgtgatgatc acatctggtt tcatggaaac tgcggaagaa atatcacaat ggtccagttt
    36781 ggcttctact cctaatttgt tcaaaacatc ctcagcagac atctgtgctg caaaactact
    36841 tcctaaacct gcgccgcata cgaataaaat gttcaatttg ttcatctttg ttctcccccg
    36901 tatcatttat tatttctcaa ctactgaaaa aagcagttgt ttgaaatctt cgatatttgt
    36961 gcattcaatc aatcgatcca acttctcttc gttgttgatc aattcaatta gttctttcat
    37021 gatgttcaga tgggagtagg agtctactgc tgccaaacaa aaaataatct ttactggatc
    37081 catttcagga ttaccgaaat cgattggttg ctcaatcgta gctacgctga ctcctaattt
    37141 attgacccca tcttctggtc gggcatgggc tagtgccatg tgtttgccaa tcacgatata
    37201 cggtccgtaa tcattcactg cacgaatcat cgcttcgaca tagcgctctt cgacaatgtt
    37261 ttctttaacc aatggtctag cagctagttc aatcgattcc tcccaagtag tggctttttc
    37321 tttgatcatg atattgctgt ccgtcaaaat atcttttaac ataggctgga tctccctttt
    37381 gttgatttct aaattgtttg tttcgaataa tttttctaac ttgccataga tgattcgatc
    37441 tactcggcca ccactttctt tcatgacatc tattatttga gtgaataact ccgtcgattc
    37501 atgactgtta ggctctaacc gctgtagttc ttcatttctt ttcaaaaagt cttctataac
    37561 caagatattc gtctcagtta aaatcggatc gacaactaaa agcggcttat ttcgataatt
    37621 caattgaaag gtgctgaaaa ccagattgac atccaattta tctactaatt cgatttcttt
    37681 tgaacttaaa actgcagtaa tctcgatctg aggaaacttt tctttcagat tctctgctaa
    37741 cagatttcca gtagccatcc catggttaca gacaacgact gatttataaa cataattcaa
    37801 gtctcgcttg attacactgg cgatcgtcga aaagtggatg acaagaaaag ctatctcgtc
    37861 ctccagtatc ttgccaccaa ttacttcttc gatcgtagga acaaaatgag tgactgcgcg
    37921 gtagattggg aaataatttg ttcggatgtt ttctttcaat ggattcatga cttggatatg
    37981 attcttgatt cttacaatca gtgcagccat gtgcttatag agattttcga ataaggcttc
    38041 ttcctttaga tggaaaggta cgtgtgtctc gttctctaca aattggatca attggatcgt
    38101 tagtaattga gcttgtaccc actcgattga attggagata tctctagcat tgaacgtctc
    38161 aatagtgaaa cgaagataat ttctttctac ttgcgggaca ttgtccaatt gaaattcctc
    38221 gatcatgtgg tcaataaatg cagaaaagct actgtctttt atttctgtat ttttccaatt
    38281 aatggcagag ataaattcat gtttcctgat tcgctggatc cagatcaatg taaaaagcag
    38341 gatttgtttt ttataaatat catcttcttg tttcaacaac ttattggaat agatctcctg
    38401 tatttttttg atttcctcca aaggaaaata atggagaaag attttttgag ccggtgttaa
    38461 ttgtgcatct tgattcataa agaagtcggt cattcccaga ttctgattga taaaatcata
    38521 gatcatcgta cggatagatc gttctgaacc tttataaacc agtccttgct ttccataact
    38581 gacgatctct atcccataac tgaccagtgt tgctcgtaac cgtctcatat cttcatctaa
    38641 tgtactttta gagatttgaa attcttcttc ttttttgttt agatggatcg ggattcggct
    38701 gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc cgattcaaaa agccgtcttt
    38761 cacatctttt atttcagttt ctaatcgagc ttgcttttcc ttttccagct tcaacaaaaa
    38821 acctttgttg cgaattgtac ggatctccgg aagcttttta ctgctcaacc agatattcgc
    38881 ttcaagaact tcctgacgaa tcgtccgctg actgacattg aactcacttg ctagatgttt
    38941 tgcttttact ggatattggg ctgtcatgat tctgtgaagt atttcaattg ttcttgtttt
    39001 catagaaatg atctccttct gatttcaagt atatggttcc gcttacataa taaaaagtag
    39061 atatttctgc cacttgaagt ggaaagaaag taagcaaagc aaacaaaaaa taagccatat
    39121 gctttttcat ctcctcaacg aaaaagacat acgcctatta ttacaaaaat tttgcccatc
    39181 tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa caactaaact
    39241 aaaatagtgc ttcctatttt ttgtttggtt gttcttatta tcgcagatgc aagcttttaa
    39301 acaccattta gtggagaagc tgtgacagag atgttcagcc ttgagcgata agaagcgatt
    39361 ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta ttgccgatag gctatctctg
    39421 gaacaccgtt tattcagaaa aaggagctgc gacaaggctt ttgtcacaac tccgttcata
    39481 aatatttcct gtcaatccag cataatcggt tgaataaaat cggcttgcca tcttttttct
    39541 ttctcagaaa aatcaaaata ccatatctga gaaaaatact gtcgttgtgt cttacttcct
    39601 gagatcaatc gcccatcttt tcgatcgact gaataatcac agcatctgaa atcaattcgg
    39661 atggcaaaac tattttcgtg gatctgtcga taatttccta cttcctgaat agttattttt
    39721 ttagtatgat ttcttacacc gtctgctttg tttttttgca gaactttctg atgttcggca
    39781 aacagcttgt ctgtataaca gcttctggct ttttcaggag tttcttcttc ccaagcggat
    39841 tggatcttca tgaatatctt ttggatttcc aaaaactgtg ctttcttttc tttagccgtt
    39901 cctgacatgc gggacaatat ctccgatttc agccttttct tttgtctttt attcttgacg
    39961 atagaaaaag cagaaacccc tcctactgac aaaacagcta atgcaagaaa ccccgtttgc
    40021 tcataactag aaggacctgt acttggacca taatacccat aagaatctct gccgtaagaa
    40081 cgactgccaa aataactgcc cccatatgaa cgaccgccaa atgaacctct gcccgaactt
    40141 cgaccgctag atgtacctcc accatgacca cccgcgacag cgaatccttg ggtcggcaga
    40201 gcaaacaaac ataggcccac taacaaaatg attgctaaat aattgatttt ttcattgtct
    40261 tcctcgttat cttttattac aaaaaactat ttttatcata gccttgctaa aaggaagagt
    40321 caataaccag atgcataaac tgttttttta gcaattttgt cttatttagt cattgagtaa
    40381 catcggacag atgtatgtgt cggcagtcca taattcctct atcttcagca tgaacaagct
    40441 tgcagacaag agatctgcgc ttcctccagg agagatttgg ttttcctgac aaaagtgcaa
    40501 cagtgctgta aatggacgat ttggtaaagc actttgacta gcagcatgag caagtttttg
    40561 catttccact aacgttttct gatcactgcg atgcaatacg catgtgtcat ctagcgaact
    40621 catcaaaact aacaacatat gcatttgttt ctgtacatat gaatcattat ctagctcctt
    40681 agctgcttgg attgcaagcc tgatatgcgg ataccctaat atcgcttctt catatgcacc
    40741 atttacgcca tatttttgct tcaccctatg accatgagtg gtttttttat gacgatattt
    40801 tgtatcagga aaagcagcaa gcttgctgac atcatcaagt attttcgata aagaaaaagg
    40861 ctgttccata ctgatttttt gtgcaatgac actgatcaat agccccattg cccaaatcgc
    40921 tcctttatgg gtattgatgc catttgtagc aatcagcatt gccttttccg cttttcgccc
    40981 aatctcagca attctttctc ttaactcctg atcaatagca taatgccatg ctatttgagc
    41041 catttcttca aaaaaaggag ttagcgtctt agcagactta tccaaaatgc gccgaccttc
    41101 ttttacggct tttgcgatcg tatggaaatt atcatctcct aaaaccatat cagctgcctg
    41161 tttgcttact tcactacctt tgattcccat agcgattcct atatcggctc ttttcaaagc
    41221 aggtgcatca tttacgccgt ctccggtcat tccgacgatt tctccatttt tctgcaaagc
    41281 tttcacgatt cgcagtttgt gttctggtgt cgttcgagca aagacgtcga cttttggtac
    41341 ttgtcgaatc aactcttctt cagacataag atccaaatcg atcccttcta aaactttagc
    41401 agtatgtttt aagccaattt gttccgcgat cgcttttgct gtatctttgt gatcccccgt
    41461 aatcattttc actgagattc ctgcttgttg gcattccttt acagctttga ctgcactttc
    41521 ttttggcgga tcaatcaacc cagcaatccc tgcaaaagtt aattgggtta aagactcatg
    41581 cgtgagttcc tgtttcgaat ctactttttt gtaagcaaat cctaaaacac gctgaccttt
    41641 tttcgcaaac gctgtggctg tttgacccca tgcttcttat tctgaagaag aaagagttga
    41701 tttagccaat aatacttctg gcgcaccttt gacgtaaatc acccctcctt tttcctccgc
    41761 atgcatcgtt gccatatatt tgtattcaga gctgaacgga atttttgctt ctattttttt
    41821 caaaggcaga tcggctgttt cggtgtggcg aatcagtgca agttctgtcg gattgccact
    41881 taaattttct gttttctgtt cttctttcgt ctttaattcc tgacaatttt tcataataaa
    41941 cagtacgcga gtttcttcct ttgctgctac atccacaact gtcatttcat tttttgtcaa
    42001 tgtccctgtc ttatctgaac agatcactgt catagaacct aatgtttcta cagaaggcag
    42061 tcctttgatg atcgcctttt cttttgccat ttcatgtact ccgacagata ggatcatggt
    42121 caacacagct ggcaatcctt cgggtaccat tgacaccacc aaagcgatca tagctgaaag
    42181 aaggatatgc caatccatgc catagcggaa tgttgtaaaa acaactaaga acaagatcaa
    42241 acaaagaatg ccttgaaaaa tttgtttgtt caatcggttg atcttcttga tcagaggcgt
    42301 cgtttgttgt tcgaccgatt gcagtgcttg attgatttta ccgatttctg tcgtgtttcc
    42361 tgtttctaca acgacaccga ctgcagaacc agattgtaca agcgttcctg aaaaagccat
    42421 atttttttgg tcaccgattg attcttctgc tgccaaaacg gaatgatttt tttcagatgc
    42481 ttctgattct cccgtcaaaa tcgattcttc tactagtaga tcatgtactt cgatcaaccg
    42541 aacatctgct ggaacgacgt ctccggcacg taagacaatg acatctcctt ttactagttc
    42601 tttcgcaggg attttttctt gcatcccgtt cgatagtact atcgcttctt gtcccatcag
    42661 ctgcttcaaa ccattcaacg attcttctgc ttttctttct tgccaataac taacgagtcc
    42721 gttgacgatc actacgaata aaataatact tccttcgatg tattcgctcg aagcaaattt
    42781 caaaaaagag gcaatgatca acacgatcat cagcagatca gtaaagtgtt ttgctatttt
    42841 tttccatcgt tttgtctgct gtcttgcttc tatctcatta tatccttgat cagtcaggcg
    42901 tttctgccgt tcttcttgcg acaagccttc tgttgatgtt tgatagtttt tcaatacatc
    42961 ttctaccgtc aatctgtacc agttcatcct atcccctcct gtttaattga tactgttatt
    43021 atacgcaggg aagttttttc aggcgtatcg attatttcag gggggtctga aataattcgt
    43081 tttgcagaat gtatctgggc ctaactgata tttctattgc ttattctttt atatacaaaa
    43141 tttaagaata caaaccagat aaccaataaa aaaggaataa agcgtgtcgt gctttcgaat
    43201 aacaagctga aagcaattga agtaaaaaac accaacaaag caagatcttt atatctttgt
    43261 gtagtgttcc gtttcttttc tttaataaat tgaacatgtg cacacaagat cgaagaccac
    43321 acaaatagaa aacaactcgt tgtcatgcct gccaaaagct gaaaaaacaa tcgactatca
    43381 gctacaaaaa aatgtagaca aagaccgaca aaaaataaca tccctgtaat taaaataccg
    43441 tgtaaaggta cgcctttctt agaaagcgca caaaaccaat ggggcaaact tttctctttt
    43501 gctgtttgat ataaaatcct gctagtactg tagactgcac tatttgctga ggacaaagaa
    43561 gcaaccaaaa tcattagatt gacaattgct gctccgcttt ggcaatggat cacttgcagc
    43621 atagtcacta aaggactttc ttttaaatct aaacaatgcc atggaacaac aaataaaatc
    43681 gacaaaatcg ttcctatata gaaaaagagg atacgccaag gcagacagtt gattgcattt
    43741 ttcaaattct tttgtgggtc actcgtctca ccaactgtta atccgatcat ttcaattcca
    43801 gcaaaactga ataaaaccat aggaaatgaa gcaatcattc cagcaaagcc aaacgggact
    43861 aattcattga gctgtccaaa atttgctttg attccaatag actgggtatg aaaatctgag
    43921 acactcaatc ctatacccaa taagataaaa aggaaaatcc ccaagatctt gatgattgag
    43981 aaaaaagatt ccaattcacc aaaccacttg actgcaacaa ggttgactgc cattaagatc
    44041 attagtgcgc tacctccgct tatcaaagct ggtacatgta caaaccagaa atggatatac
    44101 aatcctattg ctgttaattc cgcaagtcca gctccaagcc agcaaagcca ataagtccaa
    44161 ttgacaaaaa atgcccactt tttgcctaaa taatagtcaa cgaaatcact gatagagtta
    44221 aatgtactat ttgctaaaaa taaatcaccc atgacccgca tcaaaccata aatagccaaa
    44281 ccgcatatca aataaacgag aataacagct ggccctgctg atgcaattgc ctttgctgaa
    44341 cctaaaaata atcctgttcc aattgcgcca cctaatgcga ttaactggat atttcgatta
    44401 gttagagtcc gtttcattgc cggtttttcc atcaagttca cctttttctt tttcaagcag
    44461 catggcgact tcctgttccc aagaaggttt ctttattttt tcaatacaga gtggtactac
    44521 aaacaaaatg acactgatgg ctgtgactgc aattagattg atccatgtcc cagctttcat
    44581 cgtcagtccg acagaaaatc cgattgccgc aaataaaaca ataacgacag tccaaactcc
    44641 aagattgctc tttttctctt tttttccaat tctaaatggt cgattcaact ttgcatgtgt
    44701 ataacgcaat ctcaaaactg cgatccccat gatgacatag acgatacagt agataacagt
    44761 agttgaattt acgagcatca aaaatgcttg attgacgcca ggaatcagca aataaaaaag
    44821 tgcaaagatt gagataatga cagactgagt caaaataacg gatttggata cgtcaaattg
    44881 attcgtcttc caaaaattga attttggtgg atacgcacct tttctagcac tttctgtcac
    44941 tgtctttgct ggtcctgagg cccaagcaga taattggact gctacaccaa tgaagacaag
    45001 taaactaaac agattcacaa taagatgtgg caaaccaaga atttggcaat aaatcgaaat
    45061 agattgcgcg atattattca attccatttg tccttttggt acgacatttg ctacaactaa
    45121 agcattcaaa atattgacga aaaatataaa aattaacgcg gcaaatactc cgcgaatata
    45181 cgttttaact ggatttttca accgagtgat atacacagag gacatttcga taccagtaaa
    45241 aataaacatg atcggcgcaa aataaacaaa tgaggatgca gtcgtagtat ctggtattaa
    45301 tttgtcccat gaaaaactgc ctaatgttcc ggttggtacg atcccaactt tgatccacgc
    45361 agcaaaccct aataagagca tcatcgtcaa aggcacgtac aatcctaacc aaacaccgac
    45421 ttttccgccg atttttgcca tatcaaattt caaattcaaa aaagtgatga tccaataaac
    45481 gactaaaatg acaactaatg taaatttgct gttcaaacca agcggcacat tatcgataat
    45541 attaccaaac aacggagcaa gtgctgaagc gaccatcacc atcccaggaa acatttggac
    45601 ccataacagc catgagacaa cgaaccccca tttttttcct agagcattcg atacccaaag
    45661 ttctggacca cctttttggg gaaacattcc ggcaaattca ccagagatca atgagattgg
    45721 aaaagcgtac aataatactg caaccagcat gtaaaaaatc atcgtccagc tcgttgctgc
    45781 tacatctgga atatttcgta cacttgctac aagggcgcag gtcattccga taaaggtcat
    45841 ggtcgtgatt tgctttgatg aaccagtatc tttcatcttt attccttctt tcaatagcga
    45901 catatgtttt tgttttcttt tatttttgtt caagaacaga aatgacaagg tttcatcgat
    45961 tcagtacaaa acccagtatc accgaaacat ccattgaacg atcttagtga actgggtttg
    46021 atacatgaaa cgaacgatcc aacttataga acgttttata aaaaagaata tcttggcgag
    46081 gaatatatga aaaacagggg gaaatacaat tgttttgatg aagcaaataa ttgtctgttc
    46141 tccatatgtc tcttgttatt ctttcacaca aaaatcagtt ctattggatg atttcgttta
    46201 gtttcttttc cattgctttg gcgatttctt ttgtataaaa attgaaaatt cgatcatcat
    46261 tcaaataagg agtcatgata gccgcacgta gcagagtgat tttctgttct tttttccatt
    46321 cttctttcgt tagacccatt ctttcaacaa aacgaatcgg agaatcaccg taatcttctt
    46381 gtgtaaatgt agtatgagaa gtaataaacc gttcaccata aacatcgcca tccatataag
    46441 aagaccgatc gaacattttt tcatttaatt cattgatagc gttcaaatct gtacagcctt
    46501 gttctttgaa tacccaattg accatattaa aatcaggatg attcaatgga tagacttcga
    46561 ttgtttttcc tttaacagta aaagtcagat gatcgagaaa ttctctgaat ctttgagctg
    46621 cctcgattga agccccaatc aattgaccat atccggtcac attcaatggc aatacacgat
    46681 gtgctgtcca gactgcggcg gcggttgctc cagcttttga tccttccaaa atataagctc
    46741 ctaacatatc tggagccttt actgattttt caaacacata tggcgcgaaa taagaaatga
    46801 tatttctcat atttttatgt ttgattacga ttcctccagc agcataaggg acatagccca
    46861 tcttatgagg atctatcgtg acagaatccg cttctgaaat agctctgaac ccttcatata
    46921 cctctttgtc aatcgttacg cagtggtgga aaacatgatg ttcttcaaag aattcagcta
    46981 aggaagcata cggcacaaat tctcctgctt cgtttagaaa cagtgaacgc gcatagccgc
    47041 catatgcggc atcaacatgt aaatagaaat agatcccttc atcttttaac ctttctctta
    47101 attggacgat tttatctaca ctgtctactt gaccttcttc tgtcgttcct acgacagcaa
    47161 ccacacctag aattgggatt ttttgttcag ctaattcacg aattgttttt tccaaagcat
    47221 taatatccat tcgataatcc tcttgaactg ggatagcaac catctgatct aagcctactc
    47281 cgcaaatatc taacgccttc atccaagaat agtgttttgt ttgaggcacc agccatttcc
    47341 ctaatctttg gatatttttt ccgctacggg aagaagctgc tttgacctca tccatttctt
    47401 cgtccgtaaa gctttcggtc atttccaaaa tttcttcaac agataaattc aaaagttccc
    47461 actcagacat ttttttcact tgttcaggca gcacttcctt gacagctaaa ggaatcgatt
    47521 tgatgcagcg ggcgtaccat aatccttcaa gattagcaat agaaccatct gctgcaatgt
    47581 gtccccatcc gtctgtcata ttgaataatg aagcaaaatc ctgtcctact tctgcttcca
    47641 tttgcgaagt tgccatagaa gattccaatg caacattatt tgggttccac agcatcgcat
    47701 agttgtaagc taataaagca ggcatcaatg tttcagcatt catttgtccc cagtaacggc
    47761 ctgctgaatg ccatgggatc gatcctgcgc gtaatcgctg actgacttca tccaagacct
    47821 ctatcatatg gtcgcgggtg gctagataat ctggagtgag cttatctcct tcgctaattg
    47881 caggcatatc tgaaggaata taattttctc tccatccaag atgttcatct accatcttgt
    47941 tcaacaacat tttataaact ggtccatttt cagctttgtc gcctataaag acggccttga
    48001 tatccatatc tttcattttt cttccctcca tattttgttg gtcaatgtct cgcagataca
    48061 tgtgtttatc tgtaacacct cttcactata gcgccaaaaa tgagaaattt ttttttataa
    48121 catcaaatcc acgtagattt ttaca
//
LOCUS       DAFEHS010000015        40578 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFEHS010000015
VERSION     DAFEHS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.015"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(119..280)
                     /locus_tag="LP327_000881"
     CDS             complement(119..280)
                     /locus_tag="LP327_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725020.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(299..1321)
                     /locus_tag="LP327_000882"
     CDS             complement(299..1321)
                     /locus_tag="LP327_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBL1725021.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            1449..1802
                     /locus_tag="LP327_000883"
     CDS             1449..1802
                     /locus_tag="LP327_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL1725022.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            1814..2980
                     /locus_tag="LP327_000884"
     CDS             1814..2980
                     /locus_tag="LP327_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL1725023.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(3072..3692)
                     /locus_tag="LP327_000885"
     CDS             complement(3072..3692)
                     /locus_tag="LP327_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBL1725024.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(3884..4180)
                     /locus_tag="LP327_000886"
     CDS             complement(3884..4180)
                     /locus_tag="LP327_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL1725025.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            4384..5034
                     /locus_tag="LP327_000887"
     CDS             4384..5034
                     /locus_tag="LP327_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL1725026.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            5050..6777
                     /locus_tag="LP327_000888"
     CDS             5050..6777
                     /locus_tag="LP327_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725027.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            6774..8666
                     /locus_tag="LP327_000889"
     CDS             6774..8666
                     /locus_tag="LP327_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725028.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            9370..10239
                     /locus_tag="LP327_000890"
     CDS             9370..10239
                     /locus_tag="LP327_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBL1725029.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            10448..11473
                     /locus_tag="LP327_000891"
     CDS             10448..11473
                     /locus_tag="LP327_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL1725030.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            11512..12462
                     /gene="manA"
                     /locus_tag="LP327_000892"
     CDS             11512..12462
                     /gene="manA"
                     /locus_tag="LP327_000892"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBL1725031.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            12556..14259
                     /locus_tag="LP327_000893"
     CDS             12556..14259
                     /locus_tag="LP327_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL1725032.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            14261..16294
                     /locus_tag="LP327_000894"
     CDS             14261..16294
                     /locus_tag="LP327_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL1725033.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            16305..16745
                     /locus_tag="LP327_000895"
     CDS             16305..16745
                     /locus_tag="LP327_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL1725034.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(16945..18072)
                     /locus_tag="LP327_000896"
     CDS             complement(16945..18072)
                     /locus_tag="LP327_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725035.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(18075..18752)
                     /locus_tag="LP327_000897"
     CDS             complement(18075..18752)
                     /locus_tag="LP327_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725036.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            19016..20782
                     /locus_tag="LP327_000898"
     CDS             19016..20782
                     /locus_tag="LP327_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBL1725037.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            20797..21234
                     /locus_tag="LP327_000899"
     CDS             20797..21234
                     /locus_tag="LP327_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL1725038.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            21247..22398
                     /locus_tag="LP327_000900"
     CDS             21247..22398
                     /locus_tag="LP327_000900"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBL1725039.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(22460..23158)
                     /locus_tag="LP327_000901"
     CDS             complement(22460..23158)
                     /locus_tag="LP327_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBL1725040.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGASGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(23151..24503)
                     /locus_tag="LP327_000902"
     CDS             complement(23151..24503)
                     /locus_tag="LP327_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBL1725041.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(24726..26126)
                     /locus_tag="LP327_000903"
     CDS             complement(24726..26126)
                     /locus_tag="LP327_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBL1725042.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            26287..27024
                     /locus_tag="LP327_000904"
     CDS             26287..27024
                     /locus_tag="LP327_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBL1725043.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            27149..27628
                     /locus_tag="LP327_000905"
     CDS             27149..27628
                     /locus_tag="LP327_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBL1725044.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            27625..28392
                     /locus_tag="LP327_000906"
     CDS             27625..28392
                     /locus_tag="LP327_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725045.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            28622..29191
                     /gene="lepB"
                     /locus_tag="LP327_000907"
     CDS             28622..29191
                     /gene="lepB"
                     /locus_tag="LP327_000907"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL1725046.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(29295..29897)
                     /locus_tag="LP327_000908"
     CDS             complement(29295..29897)
                     /locus_tag="LP327_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL1725047.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            30003..30472
                     /locus_tag="LP327_000909"
                     /pseudo
     CDS             30003..30472
                     /locus_tag="LP327_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            30578..31162
                     /locus_tag="LP327_000910"
     CDS             30578..31162
                     /locus_tag="LP327_000910"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBL1725048.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            31165..32238
                     /gene="prfA"
                     /locus_tag="LP327_000911"
     CDS             31165..32238
                     /gene="prfA"
                     /locus_tag="LP327_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBL1725049.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            32231..33070
                     /gene="prmC"
                     /locus_tag="LP327_000912"
     CDS             32231..33070
                     /gene="prmC"
                     /locus_tag="LP327_000912"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBL1725050.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            33063..33242
                     /locus_tag="LP327_000913"
     CDS             33063..33242
                     /locus_tag="LP327_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725051.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            33264..34289
                     /locus_tag="LP327_000914"
     CDS             33264..34289
                     /locus_tag="LP327_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBL1725052.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            34464..35708
                     /locus_tag="LP327_000915"
     CDS             34464..35708
                     /locus_tag="LP327_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBL1725053.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            35861..36808
                     /locus_tag="LP327_000916"
     CDS             35861..36808
                     /locus_tag="LP327_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL1725054.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            36942..37571
                     /gene="upp"
                     /locus_tag="LP327_000917"
     CDS             36942..37571
                     /gene="upp"
                     /locus_tag="LP327_000917"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBL1725055.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(37710..37952)
                     /locus_tag="LP327_000918"
     CDS             complement(37710..37952)
                     /locus_tag="LP327_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725056.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            38073..38145
                     /locus_tag="LP327_000919"
     tRNA            38073..38145
                     /locus_tag="LP327_000919"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38106..38108,aa:Thr,seq:cgt)
     gene            complement(38264..38902)
                     /locus_tag="LP327_000920"
     CDS             complement(38264..38902)
                     /locus_tag="LP327_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL1725057.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(39025..39657)
                     /locus_tag="LP327_000921"
     CDS             complement(39025..39657)
                     /locus_tag="LP327_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL1725058.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            40008..40307
                     /locus_tag="LP327_000922"
     CDS             40008..40307
                     /locus_tag="LP327_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL1725059.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        13551 a         7161 c         8180 g        11686 t
ORIGIN      
        1 aatactcctt ttccgtcgag tctaaccatc tcggcggttt tttatttgta gaaaagaaca
       61 tcaggataga cggaaaagtc ttgtcctgat gttcttttca gatagtatgg aaagctgatt
      121 agttttctac attctgtaca tattgttcaa agtagttctg taaatcggct ttgagtttct
      181 gatattcctc ttcgctatac tctttatctt cgattttccc atcaaagaaa ggaatattta
      241 gttcttcccg tagtctgtct aatacgtctt gctgattcat ttcattctcc ttttcttact
      301 aacgatcaaa ataaacacag acacattctg gtacatagac atcttttttc agtaattcca
      361 gtaatcctgt ttcaggatga cggcgatata atgtcagatt atcggtgttc tgattggcac
      421 acacgacaaa attattgctt ggatctagtg cgaaatcgcg gggaaaatcc ccttctgttg
      481 agatgatttg cttgttttga atgtgggaac catcttcaaa aactgcgaat acagcaatcg
      541 agttatgtcc tcggttagaa gcataaagga acttgccatc atttgaaatg cggatagctg
      601 cccctccatt ttcaccgtcg aattcttctg gcaaggtcga tattttctgt aattcttcaa
      661 aagcacctgt ttctttatta taagaaagca cgcggactgt gctgtctaat tcaccaaata
      721 aataagcgaa ttgtccattt ggatgaaaga caagatggcg agggcctgtt cctggctctg
      781 cagtgaaggt agttacaaga ttcaatttgc cgttttcgga cacatcataa gtatacacac
      841 gatctgtacc aagatcacat acgactaatc gttggtcagg agtcagatcc gtatagtgga
      901 catgcgcatg atcttggttt ttatgaggac ctgtcgcttc ttgatggaat attgaatctg
      961 ctgctttcag ttcgccatct cctaatatat ggtagacatt tacttcacct ttatgatagt
     1021 tcgccccgta tagtaattga cgtgcttcat caaccgctac ataacacaaa ggagcgccag
     1081 actctgttac cgcattcaaa aaatgaaaat ccttatcgta cgcaccggca ccacctaatc
     1141 catccacact agtaactgtg taaagattgc cagctttact ttttgctaca taggtagggc
     1201 tattttcttc aacagctaat gaaaggttgg ctaattcttc tttttctgta tcaaggtcga
     1261 tcgtatagat tcctttactt tcgcgacgcg tataggttcc taaaattatc ttttcgatca
     1321 tatgagctga ttcctcctaa ttgtaatgaa atagttttcg tttttattct atcacataat
     1381 tattccacac ggctaaagaa tgagaatgtg ttatactgtt tttgaaagca attaaaggag
     1441 agagtgcaat gattcaggca aagattacag aaattgggac agaagcaata aacaaggaag
     1501 aacccatgct gattttgttc gataaaacag caacagctgc cttacgaaaa tattcggtga
     1561 ttcaagaagt atcaaaagac gaaccttttt ctttgaaagc aggcggaaca attgcttttg
     1621 atgatcaggt atatacgatc gattatgtcg gtccgatggc caatgggaat ttaaagacag
     1681 tagctcacgt atcattgatt tttgatgctt gtccagaaga aggacaaatt gcaaatggaa
     1741 tctatcttca tccgcataga ctgcctgcta ttggtttggg aagccaaatt ttttataact
     1801 aacaggagga attatggaag gaaaaaataa gaagaacaga ccggtatcgt ggttttggcg
     1861 ctggttttta aataatcaag tagtcacagc cttacttgtt gtattgttaa tattgctgat
     1921 cgtcttttta tttacaaaag tttcatattt gtttgcacca atctggcaat ttttagcaat
     1981 cgtaggtctg cctatcattt tagcaggaat cttgtattac ttgatgaatc cggtagttga
     2041 ttactttgaa aagcgaaaag taccaaggct ttacagtatc attgggctgt ttgttttggt
     2101 tgttgcacta atcgcatggg ggagcgtagt gatcatccct aagattcagg aacaaacagt
     2161 gagcttcatc agtaacttcc ctcaatatgt agatacgatt gataataaat taaccgagat
     2221 tttgcgggac ccgctgttca accaatttcg cgaacagttg gaaacagcag gtgataagtt
     2281 catgaattcg gcaggcgata tgatccaaga tatatctaag agtacagttc agagtcttgg
     2341 aagttttgtc ggcgcggtag cgactatttt agtggctctg ctgacgatgc cgtttatctt
     2401 attttatcta ttgaaagacg gtaaacaatt agcaccttat tttgtcaaat ttttaccgac
     2461 acgcatgcaa aaaccgacgt tgaatgtgct gagtgaaatg aatagtcaag tttcttctta
     2521 tataagagga caactaactg tggcttttgc tgtggcaatc atgtttatga ttggttttgc
     2581 ggtcattggt ttagagtatg ctgtcacgtt ggggattatt gcgggttttt taaacctgat
     2641 cccttactta ggatcatttt tagcgatgat tccggctatc tttttagcca ttgtagcagg
     2701 tccattcatg atcatcaaag tactgatcgt ttttgcagta gagcagacaa tagaaggacg
     2761 cttgatctct ccattagtac tagggaatca gttagcaatc catccagtaa cgattttatt
     2821 agttctgctg acttctggga aattgttcgg aatcatcggt gtgatcttgg ggattcctgt
     2881 ttatgcagca gctaaagtag tgattactca tgtttttgaa tggtacacaa caatctcttc
     2941 tctttatgaa gaaaatccgt tgaaaaagca gaaagaataa caaaaagaga cagatgggaa
     3001 aatatccaag gcacaaatgc tgctttgaag attttttcct ctgtctcttt tgtatgcatt
     3061 tatctcaatt tttaaaatga tttacctaaa gcagcagctt tatctaatgc attttgcatc
     3121 aactcatctg cacggtcagg gtcataatcg attccttcga tgaatagctg atctacttga
     3181 tcgatgccta caaagtttaa aatacctttt acatattgag aggcaaagtc ttgtccgttg
     3241 tatacaccgc cattagattg gatgtgcagt gcttttttac ctgtcactaa tccttctggc
     3301 ccgttttctg tatagcggaa cgtttttcca gctacattga cggtatcgaa ccaagctttt
     3361 aattttgttg ggatatttag attccataag gcgtttgcga taacgatttt atcagcagca
     3421 aggaactgat cagtcaattc attgaaacga gcaatttttt gttgttgact ttctgataag
     3481 gaagtgaatt ctgcaccagc acgtaatgct tcccaacctg ataataattc ttcatcgatt
     3541 tccggaatat catcgcgata aagttccaaa acagtcactt catcatctgg atgattttct
     3601 ttataacttg ttaaaaaaga cgtcaatgcc ttaactgtgc gcgattcttc tgcagttagg
     3661 ggatgacctt tgactactaa aacagatgcc atatgcttac cttctccttt tattgctatg
     3721 attgttataa ctatgactaa caaaatcagt ttactcgaaa aagaaaaggc tatcaagtca
     3781 tattgataag gttgaagaat aaataactaa aaaaagacag ggtataaaag aggctgtgac
     3841 acgatttttt gtcacagcct cttttgaatc taacaagcaa tattcaagca acactcgact
     3901 gaagagcttc catcccacca atgatataaa cagcacgaaa accattagca gtaagttgat
     3961 cggctaatgc ttctgatcgc ctgcctgtat agcttagaat ataataaagt ttctttggat
     4021 ctagattttt taattgtctt ggcaagatag cagcggggat agaaagcgag tggcttaagt
     4081 ggttttttgt gtaatctaag gtatttcttg tatctaaaac gatgctgtca gcatcgagag
     4141 gcaatttatt gaagtcttgg acactaattg attgggccat ttatctcatc ctttgtttag
     4201 tatgtggcta ttttaatggg caactctcat taattcaaat ggaaggattc ctcttatcac
     4261 ttttttaatg tatcggatga taatgaaaaa aactatgcaa atgtcttgca aaacaggtag
     4321 gaggtgttat aatgacatag cgtcatatga caatgtgtca tcttgaaaga aaggagaatc
     4381 catatgccga cacagacatt tttccatttg cctaaagaaa aacaaaaaag actaatcgag
     4441 gctgcgagaa tcgagttttc tagagtaccc ttaaaagaag cttcgattgc taatatcgtc
     4501 aaattagcgg atatccctag aggcagtttc tatcagtatt ttgaagataa agaggatctt
     4561 tatttttatt actttgagac aatgagacgt gacagctcta aagatatgtt acaactaatg
     4621 aaagaatccg atggtgatct attcaaagga ttcgaatcct atttttctaa aatgatttat
     4681 gagattttgg aaggcgagaa tgcttctttt tatcgtaact tgtttatgaa catggattac
     4741 cgtgcctcaa gaaaagtcgc tcctcatacg agtcgccaaa aaacgcctca cgatccacat
     4801 aaagaacaca aacaagatgc aaaagagttg cttgaattga tcgatcggac gaagctgaaa
     4861 gtaaaaaatg atcatgaatt ggaattactg atccaattag tgatgaatac tgtatttaca
     4921 acaattgcgc attcgtatcg cttattttca acatctgaag attactcagt agagcaagca
     4981 atcagtgaat tcaatttgaa actttcttgg ctgaaaaacg gagtatataa atagaaagaa
     5041 gggaactaga tgctaaaaat cgtaaaacgg atctccttga cttcagcaat tgcagctgca
     5101 gttttcatgg tgatccaagt actggcagat ttatatttgc cgacacttac ctccaatatt
     5161 attgataaag gggtagcgca aggagatgtc gattatattt ggcatacagg ttttgtgatg
     5221 atcggctttt ctttgatcag tatcttagct gctatcgcaa atacattttt tgctactcgt
     5281 gaatcacaga aactgggtaa gcaattgcgt acggatgtct ataaaaaatc agagagtctg
     5341 acaaaagatg cgtttgataa atatggaacg gcctctttga tcacaagaac gaccaatgat
     5401 gtgactcaaa tccaaatggt tacacaaatg tttttacgta tgatgatcaa tgcaccaatc
     5461 acgttgattg gcgcgagcat acttgcttat caaaaagatc atcagttgac aaaaatcttc
     5521 ttagttgtca tcccagtaat gattattttg atcggcggaa tcatgtactt tgctgttcca
     5581 ttattcaaaa gtatgcaaaa gaaaactgac cgtttaaacc tggtatttcg agaaggattg
     5641 acaggggtca gagtgattcg agcattcgat aagacacgct ttgaagaaaa tcgttttgat
     5701 ttagcgaata aagattatac aaatacggca atcaaagtta atacgatcgt cgcattgatg
     5761 atgccaatga tgacattgat catgagcggt acaaacgtag caatcacttg gtttggtgga
     5821 cactacatag cagatatgac tttggaagtc ggtaatctga ttgcatttat gacttatgcg
     5881 atgcagatct tgatcagctt tatgatgctt tccgctattt ttatcatggt gcctcgtgca
     5941 caagcttcag ctgaccgtat taatgaagtg ttggatgaaa aaatcggtat ccatgaccct
     6001 gaaaatccta aaacagtttc ttttgctgga aaaaacgcaa cattggcctt caatcatgtc
     6061 aattatcgat atcatggagc agaaaaactt gcattggaag atattgattt ccaagccaag
     6121 tcgggcgaga tcgtcgcaat catagggggg actggttcag gtaaaacgac cttagtcaat
     6181 ttgatccctc gattatatga catcgaatct ggttcgatcc aaataaatgg aacggacatt
     6241 agtgacatga ctcaatataa tttgcgagaa ttaatgggct ttgttcctca aaaagcagtt
     6301 ctgttctctg gaacgatccg agacaatatg cagtatggta agccggatgc aacagatgaa
     6361 atgatttgga aagcattaga aatcgcacaa gctaaagatt ttgtttcaga gatggaagat
     6421 ggcttagata gccatgtgga acaaggagga ggaaacttct ctggtggaca aagacagcgt
     6481 ctggcaattg cacgggcatt agtcaaaaca gcagatatct atgtgtttga tgattccttc
     6541 tcagcgttgg atttcaaaac ggatgccaat cttcgccaag cattgaaaac gaatatgaaa
     6601 gaaagtatca ccgttttagt tgctcaacga gtaagtacag taatggatgc agatatgatc
     6661 cttgtgctag atgaaggaaa actggttggc aaaggaacac atgaagaact attagcaaca
     6721 aacgaaactt atcaagaaat cgttcattca caactaagag aggaggactt agcatgagta
     6781 aaacaaacac acgtccctct catggaccag caggcggtcc aggacatggt ggacctggca
     6841 gaaatctcgg tgtaaaaggt gaaaagccta aaaatttctg gggaacggta cgccgattgt
     6901 ttggctatat gtctaaacgt atggttgcga tcatcgctgt atttgtacta gcaatagcag
     6961 ccgtgatctt ccaaatccaa acaccaaagg tcttggggaa agcaaccaca gaaatattca
     7021 aaggtgtaat ggctggcgct caacagatgc aacaaggcca acaagtaaca aaatttccga
     7081 tagactttga taaaatcgcg cagatcattg cgacagtaat cgtgatgtat ttgatttctg
     7141 ctgtattcaa ctttttacag caatttatta tgacacgtgt ttcccaacga acagtttatg
     7201 aattacgtca tgatttggaa gccaaaatga atcgtgtacc gatttcctat tatgatacac
     7261 atacaaatgg ggatatcatg tctcgagcaa tcaatgatat ggataacatc gcaagcacgc
     7321 tgcagcaaaa tttgactcaa tttgtaacta gtgcagtcac attgattggg gtcatcgtga
     7381 tgatgctttc aatcagctgg caattgacgt tagtcgcgtt attgatgatc cctttaagct
     7441 tgatcatcgt gatgatcgta gcgccgaaat cacaagtgtt tttcgcagat cagcaaaaaa
     7501 gtttaggttt attgaataac caagtagaag aaacatatgg tggtcatacc attgttaaaa
     7561 cattcaatca tactgaaaaa gaccaggaag tatttgaaaa agaaaatcag aatctgtatg
     7621 ccgctggttg gaaagctcaa tttatttcgg caatcatcat gccgttgatg aatttcgtta
     7681 aaaatctagg ctatgtgttc gtcgctgtac taggcggagt aaaagttgca aatgggaaca
     7741 tgacattagg ggatgttcaa gcgttcttgc aatatacaac acagttctct caaccaatca
     7801 ctcaacttgc aagtttgatg aacactatcc aatcaacagt tgcttcagcc gaacgggtat
     7861 ttgaagtgtt ggatgaagaa gagatgtcca atgaaaaatc tggccttcca gaagaaaaag
     7921 atacaccata caaagttcgt tttgaaaatg ttcaattcgg ctacacacca gataacttgc
     7981 taatgactga ttttaacttg gatgtcaaac ctggtgagat ggtcgctatt gtaggtccga
     8041 ctggtgcagg gaagacgaca ctgatcaacc ttcttgaacg gttctacgat gtttctggag
     8101 gtagtattcg ttacgatggt gtagataccc gtgatctttc acgtgatgaa ttgcgcgcac
     8161 agttttcaat ggtactgcaa gatacttggc tgtttacagg aagtatctat gacaatatca
     8221 aatatgggaa cgacgatgca acagacgaac aaatcattga ggctgctaaa gctgctcacg
     8281 tagacgattt tgttcgtaaa ttgccagaag gatataacac tgtattaaac gaggatgcaa
     8341 gcaatatttc gcaagggcag cgtcagctaa tcacaattgc gcgggcattc ttagcaaatc
     8401 cagatgtcct gatccttgat gaagcaacat ctagtgtaga tacacgaaca gaaatactga
     8461 ttcaaaaagc gatgaaccgc ctattggaaa accggacaag tttcgttgtt gctcaccgct
     8521 tgtctacaat acgcgatgca gacaacatta ttgtgatgaa tcatggttcg atcgtagaaa
     8581 caggaaacca cgatacattg atggaaaaag acggattcta tgcagatctt tataacagtc
     8641 agttttctga agaagaagct tcatagaata gtacaaattt attatttttt ttaggggctg
     8701 tgacaaaagt cgtgtcacag tccctttttc cgaataaacg gtgttcaatc agctgtcctt
     8761 cggtattagc tcaaaaaacg gcaaaatatg agaagctatt tttccgtttt gtatctctaa
     8821 tactcaggtc aaaccgctga ttttacaacc tcttccttaa acggtgttct cctgccaact
     8881 cctcggtcac aagtcacaat agtcaacaaa cctgagaagc tgttttttga aattgctcct
     8941 tgtgactcgg agttaaacgg caggctcaca acctcttggt aaacggtgtt caaaagccag
     9001 tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt
     9061 cttactactc gaaactaagc gactttttca caacctcttc cttaaacggt gttcaaaagc
     9121 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatagg aggagctatt tttgtttggc
     9181 tgttcttatt acttgatgca gaacagcttt ttcacaacct ctttttataa gagtttttac
     9241 tcattaattt acaaaaaaca agcaaaatgc gaattttctc acattttttt taacaaaatc
     9301 aaagttattg cttgaaagaa gaagaatcca tcgttaaaat aaagcttgac gtagagggga
     9361 gcaagaaata tgaaagattt tcaattgggg gctgccacct atttaaaaag tgtagccatt
     9421 cgggtgaaag accgagataa aatgattgat ttttacaaga acgttgttgg ctttgatttg
     9481 aaaagagaag aaaacgaatt agccatttta ggaacagaag aagctggaag tgaaatgttg
     9541 ctgctggaag aaagtcctag agcgaatgat cacatggggg tgatcaaaaa gctgaaccgt
     9601 ttttcattga ttatccctag cacagaagaa atggcagata tcctttgtcg tatccgtaaa
     9661 atgaattatc caatcaaaga agctttagaa gatcatggac gtatggggat tttgctcgta
     9721 gacccagaag agaatgaatt ggaaatttac catgaagaga ataagaacga ccaaagagaa
     9781 attccagaaa caatggatca agaagcatta ttaagaaaat ctgaagaaaa attcagtaaa
     9841 ctatcaacag gttcttattt tgaaaaaatc catttaaatg ttactaactt aacaaaagaa
     9901 cgccaatttc ttaatcatgt acttggtttg aaggtacata atgaagaaga gggcatccat
     9961 gtattgaatg aaggagattt ccatgttggt ttgacagaag ctagcggagg cgcaattgat
    10021 ttgcctacag atgaagtgat tggacttgat tttctgaaat tcatgatcga tactgaaagc
    10081 atggaaggac tgattgctca cttgcagcaa ctatcagttg atttcttctt agacaaaaag
    10141 cacacagtac tcacaatcta tgatgctata ggaatcgaat ggtggttcgt cagtgagcca
    10201 aaagaaaaga ataaaactga cattcgatgc gacgcttaac tttttctttc ttaacgattg
    10261 tcgcatgtct cgtcatttta tgaacggtca tactatcgct tattctagag atagaaagag
    10321 aaaatagtat accgggggaa actgcgcggt ttttcgcgca gtttttttgt gtaaaaaagg
    10381 ttgattcctc atagattaag ctttaagctt ggactgtaag aatctgatag aaaagcagga
    10441 gaaaaagatg tatccacaca cgaaacagct tattttgaaa aagatggaag aaggatttta
    10501 tccaggtgta gcagtcgcct tcataaaaga aaatcaagta gaaacattta cagccggctt
    10561 tgcccaagtc gttcctgaaa agcagctgat gacagaagat ctgttgtttg atgcagcctc
    10621 actgacgaaa gtcgttgcaa ccacttcatt agttttacgt ctattagaag atggaaagat
    10681 agaactcgat caaccgcttc ataaatatct tcctgaattt gaaaatcaga caatcacttt
    10741 gcgtcattta ctgacacata cttctgatat caaaacatgg ataccaaaca gagataagct
    10801 atctcaggaa gaattgatcg ccgcttattt gaagctagaa ccaggtgagc aactaggaaa
    10861 agaagtaaaa tatacagatg ccggtacgat tcttcttggg ttgatgctag aggaaattta
    10921 ccaagaaact gcagtggaaa tctttcaaga acaagtattg gaaccattag gaatgatgaa
    10981 cagcttattt cttccttttc cttccaaaaa aatcgttccc acagaacaac tgccttcggg
    11041 tgaggttttg cgtggacaaa ctcatgatcc gaaagcaaga gttttggctg aacatgcagg
    11101 taacgcgggt ctctttattg atttgaaaga cagcattact tttgtccaaa tgtatttgaa
    11161 caagggaaaa atccgtaatg gtcgtttttt agaagaagag acgattgatt ctttactgca
    11221 agatcaaaca ccgaaaaaaa atggctatcg ttctttagga tgggacttaa aaaaggatgt
    11281 agaagacggt catgcgattc tttttcatac tggttataca ggtacgtttt tcttgattga
    11341 tataaaaaca caagaagcgt tgatattttt gtcaaatcgc gtacatcctg tcgatcatcg
    11401 tgcggagtat ctcatatgtc gagatgaaat tgttgaaaac tttttgaaag agaaagcggt
    11461 attaccgaaa taatgctata atagctatga aaaatgagag gagtttgaat tatgcaggaa
    11521 cctatgtttt taaaaccagt ttttcaagaa aaaatttggg gaggaagtcg tttacgttca
    11581 gtctttggtt ttgatatccc taacgataaa atcggagaag attgggcgat cagtgcgcat
    11641 cctcatggtg tcagtgtagt agaaaatggc gaattcaaag ggaaaagatt agatgaacta
    11701 tggtcagaac acaaggaatt gtttggccat ccttcagaac cagtgtttcc attattgatc
    11761 aagatcttag acgcagaaga tgatctatct gtacaagtac atccagatga tgcctatggg
    11821 atgaaacatg aaggtgaatt aggaaaaact gaatgctggt atatcatcga tgctgaacct
    11881 ggtgcagaaa tcatctatgg tcatcatgca aagacaagag aagaactagc tgagatgatc
    11941 aaagatgggc gctgggatga ccttttgaag aaagtaccag ttaaaaaagg tgatttcttt
    12001 tatgtaccaa gcggaacgat ccatgctatt ggtaaaggaa tcatgatttt agaaacacag
    12061 caaagttctg acacgactta ccgcgtgtat gactacgaca gaaaagatgc gcaaggacaa
    12121 acaagagagc tgcatatcca acaatcgatc gatgtaacaa ctgtccctgc gaaaacacca
    12181 gaattacaaa tcaaagaagt ccgaaaaggg aattcatcga tcgtcactta tttagaaact
    12241 gagtttttca atgtatatga gtgggatatt aaaggaatca catctttcaa aaaacaagcc
    12301 ccttatacat tgatgacggt aatcgaagga gcaggagacc ttgtcgttga cggaaaaaca
    12361 tatccattag aaaaaggaac aagctgtatt attccaaatg gcgtttctga gtgggcggtc
    12421 caaggtgaac tagcaatcat tgcgtctgaa ccaggcaaat agttcccaga tagctgaaag
    12481 cagcagacgg tttttcgttt gctgcttttt ctacataaaa tccgtccatc aataaataga
    12541 agtctggtga aaaagatgga atacaaatgg aaagcaaaca acagcagtcg cgtgtctatg
    12601 aaacaactga gaaaagctgg acatcactta aatgaaatga tcatgccaaa tctaagtctg
    12661 tttatcgctt ggggaatctt gtcattagca acaaaaggtg tgtctggcga tttgcacacc
    12721 acattagaag aggtatgcca ttggatgatc cagctgcttt tacccatctt gatcagttat
    12781 tcaggcggga aatcggtagg gggacaaaaa ggggcagtcg ccggtgcaat tgcttcttta
    12841 gggttgatcg ctcgttccaa tgctccacaa atcgtaggag cgatgattat cgggccgctt
    12901 gccggcatcg tatatgaaca atttgtttct agattccatg tgaaattcaa ggctggatat
    12961 gaaatgcttg tcagcaacct actagtagga ctgactggta gcgttatttg tataattgga
    13021 atcgttttta ttgaaccagt tttaaatagt gttcacttgt tgctcgcctc cgttgtgtct
    13081 tggctagtca caatgaatct gttaccactt gtcagtctga tcttagaacc tctaaaagta
    13141 ctatttttca ataatgcaat caatcatggt gtattgacac ctttaggaat cgaattctca
    13201 caaacagtcg ggtactcttc cttatttttg atggaggcaa atcctggacc tggtttaggg
    13261 atcttacttt ctatcctttg ttttgcagaa aaacaggaaa aagtaaatgc gagtggagca
    13321 ttgatgatcc atgcgattgg cgggattcac gaaatctatt ttccatttgt cttgctgcga
    13381 ccgtatctat ttttagctgt aatggctggt ggggcaagcg gcacgttgat atttcaatgg
    13441 ttagatacgg gattagctgc acctgtatcg cctgggtcac tactcgtgat ttttgccaat
    13501 gcgccttatc cacaactagc tggggtaagt atagggatag caatcagtac atttgtgacc
    13561 ttcatttgtg cagcgatact attgaaatta ccaaataaag aaaaagagaa caaaacggtc
    13621 aaagaaaggg gagaaatctt ggaaccatct atcattcatt ccgtcatttt tgcttgcgac
    13681 tcaggtatgg gatcaagtgc tatgggagcc gccctgctga aaaagaaatt agcagcagaa
    13741 aaaatcgatc tttctgtcgg atacagctct atctatgaat tgaataacca agtaaatcag
    13801 ctggttatcg tgcaacatga actagcgggt cttgcaagag aaaaagtacc tcaaagtcaa
    13861 atctatgtac tggatcactt tttggaaaca gatatcttgc tgtcacgaat aaaagaagcg
    13921 atccaatttc caaagcggga agaaaaaaaa gagatagcag atatcacaga acaaaaaacg
    13981 tcgcctgcta atattgtatt tttgtataaa aacaacagac gcggctcaca aactatgggc
    14041 atgacgattt taagtcaatt agcaaaaaaa gcgaaaaaac agtttgttat ctcaaaagaa
    14101 tcaatagaaa aaatcaaaat ccaagaagaa acgatttata tcgcacaaag agaactagct
    14161 gtaactgaac agctagaaga aaagatccct cggctagttc tagttgaaca ttgggtagct
    14221 acgaatgaat acgaaaaact cgtgaaggga gaaatataag atgtatttat cgcccagaga
    14281 aaaacaatta ttagcagaat ttctcagtag cccgtcacct gtatctatcc aaaaaatgat
    14341 gaatttattg aaggtcagta aacggactat ttaccgcgaa ttagataatc ttgaagtctc
    14401 attgaaatcg gtaaatgctt ctttgaaaaa agcagctaga ggctcatttg tcattgaggc
    14461 atcagaagaa gtattagagc aattaaaaga aaatacagat gggaaaagta atgacttatc
    14521 cactgctaaa cgacaacacg cgattttagc cgagttgtta ctgacgacag agccgttgag
    14581 tcttgctcat ttcatggaat catatcgcat cagtaatacc actttttatg cagatatcaa
    14641 acagttggaa gaaagtattc gccaactgcc tttggaaatc attcggaacc agggatacga
    14701 gatcgaagga ccggaaaaat accgccggct tctgacagcg aatgtattag aattagaaat
    14761 caatgaatat gaactttttc atagtatttc tttcgatgca tcactgaatt atttttttcg
    14821 attcgttgat ccgcaacatc tatctcttgc acgcaaagta atcggggaag aattgatcca
    14881 aaaaaagacc aatctaagtg atagaaagct tgaacatttg gttttgatgc tgacattgac
    14941 gatggagcgt gtgacgaaaa atcatttact gacaaatgag acatataata gtctggcaaa
    15001 caaagaatta ctacagacag ctaaacgatt gttttcacgg atcgcttctg agacaaaaca
    15061 actttatcca gtaaatgaga tcgtcttttt tgcaggatta ttaaatgact tcacgaactc
    15121 atttgatcag aatttctttg aagagacgtt cgatacacag cttgcttatt tagtaaaaca
    15181 actgattcaa gaagtaagtg aagagacgaa tgtccatttt tatgaagatg aaactttgta
    15241 caaaatgctg ttgactcatt tatctggtgt gttttcaaga gccgttttac aagaagagac
    15301 actgaccaat ccgattttag aaaaaatcat ggttcaatac gaggaagttg cgaatgcaat
    15361 ccgtaaagca gtagagcgga tttttcaaga aaaaaaatta tccgaagaag agatcgctta
    15421 tatggtcctt cattttgcga attcattgga acgaagtccg aaagaaagca atatcagtat
    15481 tgcaggattt tcgccaagtg gattagcttc tactagcatg ttggaactac gattgaaaag
    15541 gtattttccc ttcatccaac aaattcattt ctatcggatt gctgatttaa aaaaaatcga
    15601 tttacaagaa gaatatgatg ttgttatttc tacatctatt ttaccaggat acggtggaaa
    15661 atatatttta gtctccccgc tactgctaga agatgaagtc aagcagctaa aagaagcttt
    15721 ttcgcatatt gagaagaaaa aaaggcaaaa aattacgtat aacagcaata acgagtggat
    15781 gatagaagat acttatgagg aaacaatgca tttcatggat caaatcaatc aattactcgg
    15841 tcgttttttt atccaagaac tggataatcc tgagacgatt tccgaaacga ttcgcagctt
    15901 atttccttat tttcctgaag gactagtaac ggatgaagag gtggttcatc gccggctgct
    15961 taagcgatat gaacaagcac ctatcggtat tcctcataca aatatggggt tattccatac
    16021 atccagctca ttgatttcta aaccgatctt ttgtattttc aatttgaaac atccgttgac
    16081 cattgaaggg atggatagac agccagtcga tttgtcccgt atgttagtta tgctggcgcc
    16141 ttcgccaatc caagaaaaag acgggattct tttaggaaag atcagtggag caatcatcat
    16201 gaatgacttg aacaccgaga tcttccgctc aggaaataaa gaaatcgttt atcagttgtt
    16261 agcgaaaatc ctgattgaag aaataaagaa atgaaggtga aaagatgata cagctaccag
    16321 cagaaaaaat attgctcgat caatcctatc aaaattggaa agaagcgttg accgagatcg
    16381 gccagtggat gacccaacaa gggggaatca aaccggcata tatccaatca atgatcgaaa
    16441 gacaagaaaa agcgagtgtc tatatcggga atttcgtagc cttgccacat gggactgaca
    16501 aagatgaaga agtagtagaa gaaggaatct atttagtcca agtgcctgat ggtgtggact
    16561 ttggaacaaa agaggaacca aaaatcgcga cactcttatt tgtagtggct ttaaaaaaag
    16621 aacatcagtt agcggtgctc caagatttag cctttttctg ttcagacatc gaccaagtaa
    16681 tggcgctcag tgatgcggaa accattgaag aagtccaaat gatttttgaa catggacata
    16741 tataaccaat gttcacagta aaaaaagctg ccttgcatca agtaataact tcaaccaaga
    16801 taaaaataag aggagctatt tttgttggga ggttcttatt actgcggatg caagcttttg
    16861 aaaactattt attctagaaa accgtttatt tgaaataggg gacggtgaca aggtttttgt
    16921 caccgcccct attatttttg tgttttaata gctctttttt atatagatag tgcccatacc
    16981 tatgaataat aaaacgatac tccatatacc aagtgtcaag ataccattca tgaaagtaac
    17041 ctgttctgaa gctagtggcg agaaaatatc tcctcctatg atcacatttt gaaaatcaac
    17101 ataagaagtt ggcaagtaat gactgattga ttctatccat ccaatcgaag aaaacaagcg
    17161 atactctgct aagaaaagac aaagaagtgt acctaaaaga ttgactagag aatgttcaaa
    17221 taaagcagat agaagaaaac tgattgtact taaaaagagc acccacaaca acaggaacag
    17281 aaagctctgg aaaagatact catacatcgg caggattcgt atctcctgac caatgatcgt
    17341 tcctacagga taacggaagt ctcccaaacc atttttgatt attactagta tagagacgat
    17401 caaaaatggc atcacgagac ttagcatagc tgcaccataa taaatggtaa aacggatgct
    17461 atgtttcttt accatactat ccggtaatag attgactaga gagattacct gctttctttt
    17521 ttcataagag atgatggaag aaagaaacaa ggcggttata cataaaatca aaaaactaga
    17581 aatcattcct gaaaaaatat tttcgtagta atttgctaaa gagagatgcg ctggcaagtc
    17641 aaaaatagaa tatctttgga tctgatgttc tttcatataa cggagcaact cgacacgttt
    17701 ttgttgttca atgattggaa ttccttgtct ttgaccagaa atcagtcgat ccagtatatc
    17761 tttttcataa tgataagttg cttcgaccat ttgctggaca ttccctgtgt tttttgcatg
    17821 taagattgct tcgatcagtt tgttgctttt ctctaaactt tccacaaatt ctttctcatt
    17881 ctcatcttct ttcaacgttt caatcaaacg ttgcatctct tcatagttat gattcagttc
    17941 atcgaattct gtataagcaa gtttatgatc aaaaaaaagt gggaatgcaa agagcacgac
    18001 caacaataga ggaaataaaa agtttacttt gttttttagt aagagattgc gttcaaatgt
    18061 aagtaaagac atagttacac ctcatataat ttacgataaa tagctaaaat ctcttccata
    18121 tttgaggatt ctgtgacgat ttctccattt tttagaaaac atgctttaga acaaatcgat
    18181 tggatattcg cgatagcgtg ggaagaaaga agaatcgtct tcccctcttt ctgccattgt
    18241 ctgatgaggc tattcaagat atcaatactt cctgggtcta atccgttcaa cggttcatcg
    18301 aataacatga cagatgaatc actgatcaag taagcagcaa gcaagacatg ttgtttcatt
    18361 cctagagaca tttttccaat cggcaaagta aggtactcag tcatcttcaa tcgttggaaa
    18421 acagtttggc attctacttt gctctgccaa tagtatttga tcaaagtcaa ataatcaaca
    18481 gccgatagat agtcggataa ctgattcgtt gattctagaa aaaagaaaga ccggcgataa
    18541 tctgtataag cagtaggaga gatgttgtga agggacaatt gatcataagt tgttggcagc
    18601 agttgggcaa tgccattcag caaagttgtt ttgccaaatc cgttcggggc aatcaaagca
    18661 gctacttctc ctgattctac taaaagattc aattgtcgaa tcacacgttt tttaccacgt
    18721 ttcatgttca tgtttttgat cagtaatgtc atagataggc ctccaatata aaatttaata
    18781 acataataga atggaaaaca tctaaattaa atgctaacaa aagttattgt ttgcatatta
    18841 taaaaaaatt aaaataacag ttttatttaa aattgaagtt ttctgatacc gaaatctttt
    18901 tattttgctg acatactcaa acctttggca caaagtttcg tgccaaagta cgactatttc
    18961 tctttttagt aagcggatac aattagagta tcaaaaggag agagggagag acaaaatgga
    19021 acggacgatt cccaaaaaca acatttcgct aaaagcgagg gtacaaaaat taggcagtac
    19081 tttatcaagc atggtcatgc caaatattgg agcactgatt gcctggggtg tattaacggc
    19141 tctatttatc ccagatggtt atttaccaaa tgaggctttc gctaccatgg taagcccaat
    19201 gctaacttat ctgattcctt tattgatcgg ttatactggc ggaaaagtaa ttgctggtga
    19261 ccgcggatca gttgttgggg caattgcaac gatgggtgtg attgtcggaa cagatattcc
    19321 aatgatgcta ggcgcgatga tcatgggacc tcttggtgga tatgccatca agaaatttga
    19381 tcagctattc caaaaacgga tcaaatccgg ttttgaaatg ctagtcaata atttttcagc
    19441 aggtcttatc ggttttggat tagcgttatt aggtttttct gcgattggtc cagttgtcga
    19501 tgcattgact caagcaatgg caaaaggtgt agagatcatt ttatctgccc atttgattcc
    19561 attgacaagt atttttattg aaccagcaaa aatcttattt ttgaataatg caatcaatca
    19621 tgggattttg acaccattgg gaactgaaca agtagcagag gctggaaaat ctattctatt
    19681 cttgcttgaa gcaaatcctg gtccaggact tggcgtatta cttgctttta ctttgtttgg
    19741 caaaggagca gcaaaatctt cagccccagg agccatgatc attcactttt taggcgggat
    19801 ccatgaaatc tattttcctt acgttatgat gaaaccgtta ttattcttat cagttatagg
    19861 cggaggaatg tcaggaagtt tcattttcca attgatggat gctggactaa gagcgccagc
    19921 ttctccaggg tcgatcattg cgattctagc gatgacaccg ttcaattctt accttcctgt
    19981 tattttgggc gtgattgttg ccgcagctgt ttcgtttggt atttctgcag taattttgaa
    20041 agcggatgcg aaagatacaa atgaagactt cgaaaaatct gttcaagaga caaaggcagc
    20101 taagcaagct tccaaaggtg cccaaggtat tcctcaagga gctacattct ctggcgtaca
    20161 aaaaatcatt tttgcttgtg atgcaggaat gggatcaagt gcgatgggcg cttcgatctt
    20221 gagaaaaaaa gtacaggaag cagacttgcc tcaaacggtg accaatcaag caatcagcca
    20281 actgacagat gactcgaata cattgatcgt tactcaggtc gaactacaag aaagagcaaa
    20341 acaaaaagca ccgagcgcgc gttttgtctc agtagaaaac tttttgaatt cacctcgata
    20401 tgatgaaatc attcaagaat taagcagcaa gcagaaaaca tcagtttctg aaattgagaa
    20461 tgagccagca gaagagtttc tttcagcaaa catcaaacaa atcaaagaga ttctacttgt
    20521 ccacgatgat cgttccggat cagcaacaat ggggatgacc gtgctcaaag atatattaga
    20581 caagaaccat ttaactatcc cgttaagaaa agtcaatatc caagatttaa aagatgaacc
    20641 tgattcactg atcgtagcca aagaaagttt agcggaaaaa gtacagatgg ctgcacctca
    20701 atccattcat ttatcagtca acagcatggt caatccgcaa aaatacgaat cgattgcaca
    20761 atatttgaaa cagacagctt aagggaggaa aacagcatgc aaagtttgcc attcgacatg
    20821 atcgagctca atcgatcttt taaaaccaaa gaagaagcga tccgctattg cggtcagatg
    20881 cttgtagaag cagggtgcgt tgatgaggaa tatatcgatg ccatggtaga tagagacaaa
    20941 atgttatctg tttatatggg gaattttatc gcgattcctc atggaacaga tgaagcaaaa
    21001 aaatatgtga aaaaatccgg catctgtgtc gttcaagtac cagatggtgt agattttggt
    21061 accgaagaag aagaaaagat tgcgactgtt ttgttcggta ttgcaggagt cggagatgag
    21121 catttgcaac ttgtacagca aatcgcactt tattgcagtg atatggacaa tgttgtccag
    21181 ctagcagacg cgctcagcaa agaagaagta acggaaaatc tagcaattgc ttaaggagag
    21241 agaaaaatga gagcagtaca ttttggtgca ggaaatatcg gtcgaggatt tattggagag
    21301 gttttggcag ctaatggatt tgagattact ttcgtagatg tgaatcacac gatcattgat
    21361 gctttaaatg aaagaaaaga atacgaaatc gaattagcag atgaatcaaa agaaaaaatc
    21421 actgtaaaaa atgtcaaagg aatcaacaat caaacaaatc cagaaaaagt aatccaagaa
    21481 atcgctgaag cagatttagt tactaccgcc attggcccga atatcttacc ttttattgct
    21541 gagttgattg caaaaggtat ccaagaaaga gaaaaggaag gaagcacaac atctttagat
    21601 atcattgcct gtgaaaatat gattggcggt tcagcatttc tagaaaaaga agtgtataaa
    21661 tatcttccgg aaacatcatt tactgacaaa tatatcggct ttccaaatgc agcagtcgat
    21721 cgaatcgtcc cattacaaca ccatgaagat cctttattcg tccaagtgga acctttcaaa
    21781 gaatgggtca ttgacgatag ccaaagaaaa aataaaaaaa tccaattaaa aggggtttta
    21841 tatgtagatg atctagaacc atatatcgaa cgcaaactat ttagtgtcaa tactggtcat
    21901 gcgactgtcg cttatacagg tgccttgtta ggttaccaga ccattgatga agcaatgcaa
    21961 gatgcgttag ttgtggcaca attaaaatca gttttgcagg aaacaggaag tttgctgatt
    22021 gccaaatggg ggtttgatgc agagcagcat catgcgtata ttgaaaaaat cattcatcgc
    22081 ttccaaaaca aaaatatctc tgatgcgatc acacgtgtcg ctcggacacc attacgtaag
    22141 ttaggttatc aagaaagatt tacacgtcca gtgagagaac tacaagaaca taacttgact
    22201 tgcccacatt tgacggcaac aatggggatt atttttaatt actatgatcc tgaagatgaa
    22261 caaagccgtc aattgcatga aatgaagatc catgaaaact tggaacaatt gattcaagaa
    22321 gtcacaggaa tcaatgatcc gaaaacaatc gggaatatca agcaaaacgt gaaccgttat
    22381 gccaagcaag tcgcttaaaa taggatacga aaaaggggaa tgtgacgaaa tttttgtcac
    22441 attccccttt ttcgaataac taggctgttt gtccttcaag atagggcaga tccaattctt
    22501 taccgtaacg ttgttctaaa gctaggcgga tcaaacgagc tgccaggttc tcatttcttg
    22561 caaggatagg tccatggaaa taagaaccga agacatttcg gtaaatgaca ccttcagatt
    22621 ggtcttcacc attatttcct tgaccttgta ccactttccc caaaggtcgt tcaccttcac
    22681 ctaagaaagt ccgaccattg tggttttcaa atccataata agtttcattg aattcttcgt
    22741 tatggataac gatatcaccg ataaaccggt tattttcctg acttaacgtg tagtgatcca
    22801 gtgcactgat tccttggatc ttttctccac tagctcccat gtagtagtga cctagcaatt
    22861 ggtagccacc acaaatggct aacatgacgc catcattttc gatatagttg atcaaagaat
    22921 ctttcttttt ctggatgtct tttgaaatga ttaattgctc aaagtcttga ccaccgccaa
    22981 agaaaaccaa gtcatattta tcagcatcaa aaggttcatg tatactaacg atctccgtat
    23041 ggcaagaaac ccccattttt tctgctatat atttcaacat caaaaggttt ccgttatcac
    23101 catatgtgtt catcaaattt ccataaaggt gggcgatatt gagttcataa ttagccacgg
    23161 ttcattcctc ctttgataaa tccttgagca gctagttctt tacgaagctg caatacagca
    23221 gtgtaagtag ctaaaatata gacatgctca gttggcaatt ctttgatttt ttcgattact
    23281 tctggtaatt cttttgtttc tgtcaattta ttttctggaa tcccggcaac ttttagacgt
    23341 aaagtcatat ctgtatgacg gtctcctcca gaaataacag ctgggatatc catatcggca
    23401 aagttttcaa aattaccatc ccaaatccag ctgacatcga ttccgtctgc gtaattggca
    23461 tttaataaag ccactaaaga aaatggataa ggagctagac caatcatatc gattacttga
    23521 ttgataccta caggattttt gacaagtact aatgtacatt ttttcccatt gatattgatg
    23581 acttcttgtc ggccgaatac tttctcgtca tatcctaaac cttgtttgat tttatcagga
    23641 tcgacattga agtactctgc cactgcagta gctgctaaag cattgtagac attatacata
    23701 ccgccaactt cgattccgta ttcatgacca tcgatcacga aatctgctga cacattgtcc
    23761 atccgtacca tatcagttaa tcgataatcc aattcaggac gtttgaagtc acaatttgga
    23821 cagtaatatt tccctaagtt tgcataagtg atcattttat aatgcaagat gtgatgacaa
    23881 tttggacata agacaccgtc tgtattgtaa tgagccattt gttcacgatc ttcttcatgg
    23941 tcaaagccat aatattttct aggattgact gtttcgatag aattgaagat tggtgaatca
    24001 ccgttactga taatcgttgc atttggtgac tttgcggcac cgtctacaat cattttatag
    24061 gtcgtataga tttctccata ccgatccatt tgatcacgga aaatatttgt aaataagaac
    24121 agttctggtt tcatatattc tgtgactttg ctcaaacttg cttcatctat ttctaggaca
    24181 gcaaactttt tctgtccttt ttttggtttt gcctgcaaga aagtcgatac aattccctgt
    24241 accatattcg caccagtagg gttggttaga acttcatcga attgctggcg caaaatattg
    24301 actgttaaag cggtagtcaa tgttttaccg tttgttccgg tgacaacgac gacttggtag
    24361 tcttttgcca aactatctag tatatgtgga tcgactttca gcgctagttt acctggtaaa
    24421 cttgatccgc ctttaaaaaa agtttttaat gcccattgcg acgtttttcc aactgcaatc
    24481 gcaaaatgac tgcgtattcc cattatgtaa cctccaaaaa cgatttgctg acgaataaat
    24541 tcaactaatc ataccatatg ttgaaaaaaa ggttaaattg cttttcttat tcttaagaaa
    24601 aggttacggc tgaaagtgca tgaaatcgga caaaatcgca tgatttaaca aaaaaacagc
    24661 ttgaaacaaa atgattgttt caagctgctg tgattttatt attccctctt acctatattc
    24721 ccattttaac cgataataat tttctcgctt ggatattcat agcctagatc tcgtgtttct
    24781 tttggcacga ataacatcaa tgtatatagc attccgatcc gcccaataaa catcagtgta
    24841 gcaatcgtga ttttgcccac aacagacaag tctccggtaa tccctaaaga taaaccagtt
    24901 gttccaaacg cagaagttac ttcgatgatg atcgagatca gcgtttgttc ttctgtagca
    24961 gataaaaaaa cgatacaaaa gaaacacata ccaagagaaa gcataaagac aacgactgat
    25021 tttcgcacat catcttgatc gattctccgt ccaaaaatat tgatgttatc ttcacttttg
    25081 aggaaggaat atagatacaa accgatgatt gctacagtag ttgttcgaat cccgccgcct
    25141 acagaactag gactgcatcc gataaacatc agtaaagaaa aaatgatcag tgttgtgatt
    25201 tgaaaatcac ccaaatcgtg aatctgcaga ccagcattac gagtcgtaat cgaataaaac
    25261 atcgaattga tccatttgac actcatattg gaatcttgga ataaatggtc cttttccaat
    25321 agataaatca agacagttcc cgaaacaaac aagataacaa aagctaacac agctaatttg
    25381 gtaaacaagg aaaaacgaaa cggaagcttc gcattggaac gtttgtataa tagccattcc
    25441 cggcactcca tcaatacagg aaaaccaatt cctccgacaa aaatcaaaaa catgatcagg
    25501 atcaagaaaa aatagtcatg tgcaaagggc ttgatggaat ccccagtgac gtcaaaccca
    25561 gagttcgtga cagccgatat cgcctgataa aaaccgtaaa aaacggcatc acgccaacga
    25621 tcaaaatagc ctctgtaata aaaataaata gaaaagaatg tcccaaaaag taattgaaac
    25681 cagattaaaa tagcaaaagt gatccgaatc agtcggacga taccagacaa acgcggttga
    25741 ttcatatccg tcatgatcag ctgtcgctgt ttcagtgtga tccgtcgttt agaaaaaatg
    25801 acaaaagcag tagaaatcat catgatcccc aaaccaccaa cttgaaataa tatttccagc
    25861 agtataatac cgttatcatt gaacacagaa ttgatatcaa aagtcgatag tccagtcaca
    25921 ctcactgtac tgattgccat gaacagcatg tccacaaaag aaacatggct atctggttcg
    25981 cgaaaaatcg gcatataaaa tagaacgagt gataagaccg tcatcaatat ataataaaaa
    26041 acgatgattt ggatcgatga gaaatgattg gcagcaaact tctgtcctcg ccgctgcagc
    26101 cttcgaaaga ataaatcaac atgcacagta ctatcctcct gacaatagtc gtttctacca
    26161 gtatacaagg ggaattagga atactcaaag aaattatcaa acagataaca atacagcttt
    26221 cgattaaaat agaaatggct tataataagg aaaatcctag aaaaaagaag gaggaggaat
    26281 aacaaattga ttaatgctca acaaatcgta gaaaaaatga aaaatcaaaa aataaattat
    26341 gatacagttc tgaaaaaaat gatcatgcaa tgggagaaag aagaagtaaa gcctaagata
    26401 ttaatacata catgctgtgc tccttgcagt acgcacacat tggaattcat gtctcaatat
    26461 gcagagatca cgttattttt ttcaaattcg aatattcatc ctaaaagtga gtatttgcga
    26521 agattacatg aacaaaaacg atttgtagac caatttaatg agaaaactga ccatcatgtt
    26581 gcgctaatcg ttgatgaata ccgtccaaat gaattcacca aaatggtttt aacaaaccat
    26641 cttgaaaacg aaaaagaagg tggtgctcgc tgcagtgcat gcttcaatat gcgattagat
    26701 cttgttgcgc aaaaagcgca agaactagga tacgattact ttggaagtgc cctgaccatc
    26761 tcgccaaaga aaaatgcagc tttgatcaat cagatcggaa tggatatcca aaagatttac
    26821 gggacacaat acttgccaag tgatttcaag aaaaacaaag gctatgaacg ctctttgcaa
    26881 atgtgcaaag agtatgatat ttatcgccaa tgttattgtg gttgcgtgtt cgctgccaag
    26941 cagcaaggat gcgatctgaa agaaatcaat cgggaagcaa aagcttactt aaaaacatca
    27001 accgtagttt ttgaggaaaa ttaaagaaat ctttcggaaa aacgtaagaa aaaaataaag
    27061 aatttatggt agaaataagg tagactgtat aatttgtccg tagtaaagta aggaaacaag
    27121 aataaatttc aatgaaggaa gagataagat gcgaaaagta gcacctattt tgaagccaat
    27181 cgctgtatta acaatctcga cgattgtcgc gactactaca ggtattgtga atagttccag
    27241 ctaccgtgca ccaagttcaa gttctagtac tgtagaagaa acaacaccaa ctacttcagt
    27301 ctcgacatct gaaccaacaa caacggaaag tttgaatgaa accactacga caagtacatg
    27361 ggacgataca acaactagtt cgtcgtatga tgagacatat acaactgaca gctcaagcga
    27421 aacaagtaat acggtaccag atggcactcc atcggattca accaatccaa gtacaagtcc
    27481 ggatacaagt gctagctccg aaacgatacc aagcagtacg actgtagatg ataacacgca
    27541 aaatgatgat gcagcacgta cagcacagca aaaccaagat tcaggaaata cccaaacgta
    27601 taaccagcaa gcaggtgatt ctcaatgaca tttttactag accttatcaa cggggtgcat
    27661 aaattccttg gctatttaga catcagtcct aaatatctta accgtggata tacgatttta
    27721 agtgtgattc ccacgcttta tttattgaga attgtttatg gactttggca aaaccaaaac
    27781 tatcttcaat ttttcttata tggacttgca tttctagtgc ttgtttattt tactgtttta
    27841 aatgtatttt attacttttt agataaaaac acgaaagccg atgtcactca gctttttgtg
    27901 aagtatttac ctgatgaagc gttcaacatc cagacagaag aatcgataga taatggaaat
    27961 tcgatcgata aagtgaatac acaagaggtc atggtttctt atgacgaaga ttatcaattg
    28021 aaattagccg agaatatgcg gtatttgatt ggaaatggtg agatcaaaac aaatgaactc
    28081 ggtcgcatgg atggatttct ggtcgataga aatacattgt atccttatta ctatgtcaag
    28141 aaaacaggag agaaagagta tgttctaaat attggacgaa gctactcgga catggaaaaa
    28201 gtagggacca ttcgacttga ctcaacagaa gacacattgg aacctgtcgg attgttcatc
    28261 gtcggcggtg attttgtcaa agacgggtta cgctatcatg agccttatcg tctgaaattg
    28321 attgcaaaaa aggcacagtc gcaggaagaa ccagtggttt atagtcgtag ccaaagaaga
    28381 agacattcat aatgtgatga aacgccgtgt atcagtggtt gcgataaaaa cgtttattcg
    28441 gaataagggg gatgtgacac gtcttttgtc acgttccttt tttatataac gagaaagata
    28501 agacgaacac atcacttatt ctttcctttc atttataaac attgtagaat aaagaaagta
    28561 caacgaagta ttgtgaaaaa acgtaataaa taacgaggaa aatacaagta ggaggtaagc
    28621 tgtgaaagaa tttttgaaaa attgggggat tttgatcctt gtattagcgg ctattttatt
    28681 agctagagtg tacgtgttta caccagttac tgtaaacggg cattcgatgg accctacatt
    28741 aagtgatgga caacgattga tttcatctaa gatctcaaac tatgaacgaa tggatattat
    28801 tactacaaag gaaccggggg atgaagaacg gatgattgtt aaacggatca tcggtatgcc
    28861 tggagataca gtcaagatgg aaaatgacca gttgaccatt aatggcaaaa agtatgatga
    28921 accttatctg gatgagttca aaaaagaatt ttcagaagat aagttacaag gagaatatgc
    28981 ttatagctca ggattccaag cacaagcaga aagttcatcc acctttacga gtgactttga
    29041 atatactgtg ccaaaaggaa agtatcttgt tctaggcgat aaccgcttga tttcaaaaga
    29101 cagccgaatg tttgggttag tcgacaaaga tatgatccaa ggaaaagttg ttttccgtta
    29161 ttggcctctt tcagaaatta aaatcatgtg atgaaaaaat aaaaagaagc tgtgacatgt
    29221 tttttgtcac agcttctttt tccgaaaatt ccttcttatt tcttggagtt gctcactttt
    29281 tttgcaatct cattttaagc tttttggctg atggataaga aaagcgaagc gtttttgttc
    29341 ataaatagct tttccccacg atggaaggta tgactagtag cagagacaaa gcccgttttt
    29401 tccaatcgat tattcaattc ttcttggcta aagccattat ggactttcgg atgactaatt
    29461 tgttcattct tgtcaaagtc tacgatgatc aattgaccgc caggagctaa aatttcataa
    29521 agttttgtaa gaatattctc cgtatcagga atgtgtaaaa gtacaagtga tagaaggatg
    29581 agattagctt ggacagcagg aaattcgaca gaagcatcag cgtggatcgt tcggacattt
    29641 ttcagatctg ctgcttggat cttttcttct gccattttca gcatagtttc tgaagcatcg
    29701 gcaatgatca gttctttgaa tctttccgca agcggtaaac taacaagccc agttccgcct
    29761 ccgtaatcca gcaggacttt ttggtggctg tcgcgcggta gataagtagt tagttcttcg
    29821 cgaataatct tggctaatgc ttgacggtca ggggaatcat aatggttagc gatggaatca
    29881 aaaatatttt ttgtcatcaa atagctcctt acgtaaaact ttgaaatttt aacttattat
    29941 agcacgtgaa aagaaagaaa gcatgctata ctcaattcaa aaagggagaa tctagaaaga
    30001 atatgccgat atcgtttgta aaagatagag aggaaaaagg aaagtgtgtc cgagaaatat
    30061 tactagactt gcctgagtgg tttgggcttc ctgaatcaac ggaaaaatat atcgaggaat
    30121 caagtaaact acctttatgg tgtgaaaaaa gaaaagaaga atatttaggc tttatcactc
    30181 tttcacagac aagtgaagat actgccgaaa tctattctat tgtatgggaa taaaaaaaga
    30241 ctatcatcac caaggattag gaacaaaact ttttaaagaa gcagaaggtt acgcagcaaa
    30301 acattacaaa tatttgcaag tgaagactgt agacgagggg cactactcta tatatgatca
    30361 aactatttgt ttctacgaat cattaggatt ttcaaggttg gaagtttttc caaatttatg
    30421 ggatgaatgg aacccatgtt tagttcttgt caaaaaattg gaacaaaagt gagcaataga
    30481 tttaatacta atctgtgctt gatgaaaatt ctcactgtaa aaaatgctga aaatcaagta
    30541 taataccata ggaacaaact ggaaggtgaa aaagagcatg gcacaactat tttttaagta
    30601 tggcgcaatg aatagcggca agacgatcga gatcctaaaa gtcgcacata attatgaaga
    30661 acaaaataag ccagttgtct taatgacaag cggtattgat acaagagatg gtttaggcgt
    30721 tgtttcaagc cgaatcggac tgaaacgtaa tgcgatgccg atatttgaag aaacagacgt
    30781 atttgacgta attcagaaaa tggaaagcaa accctattgt gtgctgatcg atgaagcgca
    30841 attcttaaaa aagaaacatg tcattgcttt tacgagaatt gtggacgaat tggatattcc
    30901 ggttatggca tttggtttga aaaatgattt tcgaaacgag ttatttgaag gatcaaaata
    30961 tttattgctt tatgcagaca aaatcgaaga aatgaaaaca atctgttggt tctgccacaa
    31021 aaaagcaatc atgaatttac attatattga tggaaaccct gtttatgaag gcgatcaagt
    31081 gcaaattggc ggaaatgaag cttattaccc cgtttgtcga aaacattact ttcacccatc
    31141 aatgattaca gaaggagaat aagcatgtac gatcaattac aagcaattga agaccgttat
    31201 gaagagcttg gagaactttt aagtgaccca gaagtgattt ctgatacaaa gcgttttatg
    31261 cagctttcaa aagaagaagc aaacacgcgc gaaacagtcg aagtttatcg tcgttataaa
    31321 gaagtagtac aaggaatcac cgatacagaa gaactgcttg gtgaaaaact ggatgatgaa
    31381 atggctgaac tagcaaaaga agaattatct gaattaaaaa aagaaaaaga agtcttagaa
    31441 gaaaaaatca agatcttgct tttaccaaaa gatccaaatg acgataaaaa tatcatcatg
    31501 gaaatccgtg gagcagcagg cggagacgaa gcagcattat tcgctggcga tcttttcaat
    31561 atgtaccaaa agtatgctga agcacaaggc tggaaaacag aagtgatgga agctaacgtt
    31621 accggaatcg gtggatataa agaagtgatc atgatgatca ccggagacaa cgtatattcg
    31681 aaattaaaat atgaaagtgg tgcgcaccgc gtccagcgtg ttccttcaac tgaatcgcaa
    31741 gggcgtatcc atacttccac cgcaacagtt gtcgtgatgc cagaagctga agaagtagag
    31801 atcgatattg aagacaaaga tatccgtaca gatatctatc atgcttcagg tgctggcgga
    31861 cagcacgtca ataaaacagc atctgctgta cgtttgactc acttaccaac agggatcgtt
    31921 gttgctatgc aagatgaacg ttctcaaatc aagaaccgtg aaaaagcaat gaaaatcttg
    31981 cgtgctcgtg tgtacgataa gatccagcaa gaagcacaaa gcgagtatga tgccaatcga
    32041 aaatcagcag tcggaactgg ggatcgttca gaacggatcc ggacgtataa tttcccgcaa
    32101 aatcgtgtga ctgatcatcg tatcggatta actatccaaa aattagatca gattttagca
    32161 gggaaattag atgagatcgt tgatgcattg atcatgtatg atcaaacatc aaaactggaa
    32221 gctatgcaaa atgggtaaaa tgacctatcg agaagtcctg tcacgggctt cttctttttt
    32281 agaggatcat gggaaagaag catatagtat ccaattttta tttttggaac gaaaacaatg
    32341 gaaaaaactt gattggctcc ttcatatgaa cgaagagatt tcagaagaag aacagcggtt
    32401 gatcgaaacc gatctgcgac ttctgcttgc agatcatcca cctcagtatt tgctaggata
    32461 tgcagatttt tatgatcatc ggttaaaggt aactgaagca acactgatcc ctcgaccaga
    32521 aacagaggag ttagtcgaat ggtgtttaga cgagacgcct ggtgtaccgt tagaagtgat
    32581 agatattggg acagggactg gagcaattgc tattagtttg aaagcagcaa gaaaaaattg
    32641 gcatatatct gctgtggatc tttcagaaga agcattagaa gtagcgaaag aaaatgctca
    32701 gaaagaagga acgaaaatca gtttttatca tggagataca ttaggaccag taatggatca
    32761 gcaatttgat gtgattattt ctaatccgcc gtacatcagt cggaatgaat gggaactgat
    32821 ggatgaaagt gtccggtcat tcgaacctaa aatggccctt tttgctgaga acgatggatt
    32881 agctatttat gaaaaaatag cagttgaagc accaagcgta ctaaaatcaa atggaaagat
    32941 ttttttagaa atcggatttc gccaaggtga agcagtaaaa aaaatatttc agcaagcatt
    33001 tccagataaa aaagtggcga taaaaaagga tctttttggc aatgaacgaa tgatccaagt
    33061 ttatggttga agttgtggat gaaaaatttt ttatttgttt ttttattata aaaaaacact
    33121 tatttgacaa ggatgtaaac gactttttgg tttatcaaca agtttatcca ctattgacaa
    33181 atagattatc cacaaacagt ggaaaacttg aggataaact cagacaaaat aagaatgtat
    33241 gaaaaagggg ttgtggataa actgtggaaa cgaaacgata tgatgaaaca gaattgcaag
    33301 aggcagccga agcactgaaa gaaggagaac ttgttgcttt tcctaccgaa acagtatacg
    33361 gattgggcgc caacgctctt ttaccaaacg ctgtcaagaa agtgttttct gtaaaaggga
    33421 gaccgcaaga taatccatta atcgtccatg tcgcctcttt tgaacaagta aaagagtatg
    33481 tggacaactt tcatccggca gcagagaaga ttgtgaagaa cttctggcct ggaccattaa
    33541 ctttgatatt taagatcaaa aaagacactt tgccctcagt tgtgacggga ggattatcca
    33601 cagctgcatt tcgtataccg gataacaaaa aaacactaga agtaatcgaa ctttccggtg
    33661 ttccgcttgt cggtccaagt gcaaatacgt cgggaaaacc aagtccaacg actgcagatc
    33721 atgtatatca tgatctacaa ggaaaaatta ccgggattat tgatgatgga gctacgcgaa
    33781 ttggtgtaga atccactgtt cttgatttaa gtgatccaac agccatgcct atgattttac
    33841 gaccaggtgc agtaacgaaa gaacaaatcg aagcagtcat cgaatctcct gttgctattg
    33901 atcagcatct tgtaaaagaa aatgaaacac caaaagcccc tgggatgaaa tacaaacact
    33961 actcacccga tacacgtgtt ctgatggtca gagatggcga ttggtcaact gcagttcaat
    34021 gggcaaaaaa taagaagatc cgtgcaggtg tcatagcaag ccccgaaatt gcggatcaag
    34081 tgcggacaga tactgcagct gtttatatgt ataacgacaa ctcagtagaa gcggcggcta
    34141 aaggattgtt tgcaggtcta agaggattag atgaaccaac acttggatta gatttgattt
    34201 ttgtacaagt gtatccagaa actggattgg ggaatgctta catgaatcgc ttgaaaaaag
    34261 cagcaggaca aaattatttt gaaaaatgat caattggtta ttgtcgcatt cttttagtat
    34321 gatacaatgc tatcaagtaa atcttctatc agtgaacgaa aaactaagcg taactaatcg
    34381 taatagaaga gatacgaata tttggcaatt tgtttttgat gggaaaaata aaattgtagg
    34441 aaaataaaaa ggagctgttg tttgtggtag attacaaaac gtttgaccct gatctttggg
    34501 ctgcgatcgc caaagaagaa gaaagacaag aacataattt ggagctgatt gcctctgaaa
    34561 actttgtctc cgaagcagtg atggctgcac aaggaagtat cttgacaaat aaatacgcag
    34621 aaggttatcc agggcatcgc tattatggtg ggtgtgaatt tgtcgatatc gttgaaaatt
    34681 tagcgattga ccgtgcaaaa gaattattcg gagcgaaatt tgcgaacgtc cagcctcact
    34741 ccggctctca agccaacacg gctgcttatc tcgctttggt agaaccaggg gacacgatct
    34801 taggaatgga tctttccgca ggcggacatc taacacatgg atcaccagtc aacttcagtg
    34861 gcaaaaccta tcactttgtt gcttacggtg ttgatccgac aacagaagtt attgattata
    34921 atgtggtacg tattttagct agaaaacatc aaccaaaatt gatcgtagca ggtgccagtg
    34981 cttacggacg aacgatcgac tttgcgaaat tcagagaaat cgcagatgaa gtaggcgcaa
    35041 aactgatggt agatatggca catatcgcag gattagtagc agcaggactt catcctaatc
    35101 cagttcctta tgcagatatc acaacgacta ccactcataa aacattgcgt ggccctcgag
    35161 gcggcatgat tttgacaaac gatgaagcat tagcaaaaaa aataaatagt gcggtatttc
    35221 ctggtattca aggcggccct ctagagcatg tgattgcggg taaagcagta gcattcaaag
    35281 aagcattaga tcctgcattt aaagaatata gtgaacaaat catcgccaat gcaaaagcaa
    35341 tggtcaaagt tttcaatcaa gcaattggaa ccagagtcat ttcaggggcg acagataatc
    35401 atctgatgtt gatcgatgtt cgcgagttag gtataaatgg aaaagaagca gagagcattt
    35461 tggacagtgt gaatatcact gtaaataaaa actcgattcc ttttgaaaca ctaagtccat
    35521 tcaaaacaag cggtattcga atcggcacgc cagcaatcac tactcgcggg ttcaaagaag
    35581 aagatgctgt aaaagtggct gaactagttg tcaaagcatt acaagcaaaa gatgataacg
    35641 ctcaactaga cgaagtcaaa acaggtgtac gcgaattaac tgaaaaattt ccgttgcata
    35701 aaaaataaaa atttaaattc cattagtaaa cagaaaagtt caatgttggg cttttctgtt
    35761 tattttttgg gaaaacggat tctttgacaa tatcggtgat gctctctagt atatgaggtg
    35821 taattttagt cttcacacta aatctcaggg aggaaaagtc atgactgatc gtatctcgtt
    35881 agaaaaggaa gcagtagcat tcagtgatgc aaatgcgcct catccgcgta tctatgaact
    35941 agcaccggaa gaaggaagag ttttactaga agaagtacaa gaatcacctg tgaacaaata
    36001 ttccgtcgat attgaagact tgatggtgga tactggcgaa tggggaaaac tcaatattcg
    36061 tctcgtacga ccagaaggga atatggataa attaccagtt attttttata ttcatggagc
    36121 aggctgggtt tttggcagtg ctcaaaccca tgataaattg atcagagaac ttgcggttcg
    36181 tacgaattcc gtcgttgtct ttccagaata tagtcgatca ccagaagcaa aatatccaac
    36241 agcaattgaa caaaattatg ctgtgttgca acagctgaat aaattagcca agtcaaaaaa
    36301 tctggatctc gagaatctaa tcgttgcagg agattcagta ggtggtaaca tggctacagt
    36361 catgacgatc ttaacaaaag aacgaaaggg cctcccaata tccaagcaat tgctgtatta
    36421 tccagtcaca aacgctgatt ttgatacaga ttcctatcat gaattcggag aaaactattt
    36481 tttgacaaga gaaggaatga tgtggttttg ggaccaatat acgacgaatg aagaagagcg
    36541 tgctgaaatc acagcttcgc ctttgagagc aaatataaat gaactaaatg atcttccttc
    36601 agcaatgatt ttaacgggag aagcggatgt attaagagat gaaggtgagg cttatgcgcg
    36661 caaacttcgt gatgcagggg ttcctgttac acaagtacgc ttccaaggaa tcatccatga
    36721 ttttgtcatg gtaaatgcta tggatcagac aaaggcaacg cgtgcagcaa tggcattatc
    36781 tacttcttgg atcaagaaaa aagaataagc tttgcttgtc cgcaggaaaa cttcttttca
    36841 aaaacggatt ttcttaggaa aaatggcgag tttgactagt caatcacttg attttgtttt
    36901 acaattaaaa agaaacgaca gaacaaagga gagcttaaaa aatgggcaaa tttcaagtga
    36961 tcgatcatcc attgattcaa cacaaactaa ctattattcg ggacaaaaac tgcggaacaa
    37021 aggtttttcg tgaagtagtc gatgaaatcg ctatgttgat ggcatatgaa gtatcacggg
    37081 atatgccatt ggaagacatc gtgatcgaaa caccgatcac tgaaacaaca caaaaaacgt
    37141 tgtctggtaa aaaagtagcg attattccaa tcttgcgcgc aggtattgga atggttgacg
    37201 gtatcttgga attgattcca gctgctaaag tgggacatat cggtctttat cgagatgaag
    37261 aaacattgga accacatgaa tatttcgtca aacttccaga agacattgat gcacgtcagt
    37321 tgtttgtagt agatccaatg ttggcaacag gcggatcagc aatcatggca atcgatgcat
    37381 tgaaagaacg tggcgcgtcg aacattaaat ttgtttgttt ggtagcagca cctgagggag
    37441 taaaagcttt gcaagaagcg catcctgata ttgacatcta tacagcttct ctagatgaaa
    37501 aattgaatga acatggttat atcgttcctg gactagggga tgcgggtgac cgtttgtttg
    37561 gtacaaaata agtaaatacg aaaaagggtg tgacacaact tttgtcacat ccttttttgg
    37621 tcatttttaa cgtttgatgc agaacagctt tttcacagct ttcgtttgca tataaaaaaa
    37681 ccatcccaaa aatgcagaga tggtcaaatt caaatctttt ttagttcttt gtagttaacg
    37741 attactacac ccttactttg cgtcatcaag ttcgcatttt ctgcagtttc atcaatctca
    37801 acaacagcgg aatttttcaa ttgctttgaa atcgttccag tttgcgactt gccgtgaatt
    37861 gtgaacatta cgtgagtatt gatcttgaat ttttcaccaa tttccgatgg tacgatagtt
    37921 gtatcaaatt ttccaaaagt tgccattttc ataaaacctg cctttcacat caattatacc
    37981 acgttttatg aaaaatatca tgaaattaaa ctaattttcc atcggactct ttacaatcag
    38041 ccaaatagag tatataattt cttttggata aggccagctt agctcagttg gtagagcaac
    38101 gcactcgtaa cgcgtaggtc gtaggttcga tccctatagc tggcattaaa agagaagaca
    38161 gatatatcaa ggatttttaa tccattgata catctgtctt tttgtttaaa ggaaataaag
    38221 tatgctttct tagcaataga gcatgtatta tttctataaa aatttaggag aataaaacga
    38281 ctagcaatat agctgtgaaa tcaaatatca catgaactgc ccaacttgta atgatcgagt
    38341 tgcttttaat ggcagcttgg ttgaataaca atcttgccac cccttgtatc aaaaggatgt
    38401 gtgctacagt gtgtaaaata tttccattat cataagtact gtaatggata aatccaaaga
    38461 tgattgctga caagaaacta gcgacaggaa tgctgaactt actagaaaaa atagcgagaa
    38521 aatacactga gaacaattct tctccaagta tcatgatagg caaaataata aataataacg
    38581 ggctgaaagc ctcatttatt ggattgcctg ttaaacccca tttcaaaatt tgttgcaaaa
    38641 ataccgagac tgcggcactg attactacat tgataaaaaa atatttggct atatttttga
    38701 caggagcgac aggtttcgag aacatttttt tcgtatcttt tttaccaaaa acaagtgtaa
    38761 atgctataaa tccagtgata gttaaagaaa aaatgctaat aatggatggc gcaaatagca
    38821 accctaacaa accgagtaca acgacaatag tgcccaatat ccctttttta agttctattt
    38881 tttttcctaa aatgttattc atgataataa ctcctactat tcagtttttg aagatagagt
    38941 cagtatacat gaagagttaa aataggcaat gtttttttat ggatatttgt attgctaaaa
    39001 atttatctta tatattcatt ttgattatac tacttttatt tgtaaaaatt ttctagcaaa
    39061 accaagtgct tgcaactgat taagcgtaca attttttaga tgttccgagc gtacttcaat
    39121 catttcaaag gagttgctac ttaaatcgat attttttaaa tcagtatcaa aaaagctagc
    39181 attttctaaa tcattagtag aaaacattat tttagtgagg gaacagtcta caaaatctgc
    39241 gcttgataag acagagtgct caaaggtact ttcttttatg tttgaaccag ataaatttac
    39301 atagtccatt ttacaattct cgaataacgt gttcccaata taactttcca tcaatttagt
    39361 acctgttaat ttacaattga caaatttcac gcgatgcaac atgctttttg tcaatataca
    39421 gcctgaaaaa tcacagtttt cgaatataca gtcggtaatc tccattcttt gaaaatcatt
    39481 ggaagcaaaa aatgcatact tgaaaataga tttgtaaata attggatggc agattatttg
    39541 gttttcgatg acctcttttt gtgaaaggat attatacagt tcaaaatctt cgttttcata
    39601 gatatgaaaa aaggatcctt tattcaagct tgcttcatta actttaggaa aaatcatatt
    39661 taattacctc cattttcata aagtcatatt aacaaaagct aaaccaactt acattagcag
    39721 aaaatgagat aggttttcgc ctttttcgat aacgatagtg tcgctatctc ttgaaacgtt
    39781 cttagaattg ccctgagaga cacaaattag gcgtaattct aataaaaatc ttagaataaa
    39841 aatttttact gatttaattt agaatgtttt taaaataaaa atcgttgcca taacagtgta
    39901 ttgaaaggtt ttaacaatcc agtaatgatt taaaaaaatc tagatgtagc gtatactttg
    39961 tttatagaat gtagtgggaa gaaatatatt tttgggagga aatcgtgatg tctaaaagtg
    40021 aagaaataga aaacatggta ctaaaattat atgatctcgt tttaaatcca gagatcaaag
    40081 aaaaggaacg tatacttttg atagacgcaa agacaggctt agaaaaaggt cagtattatc
    40141 ctaaagtcat aaataatttg gaacgtagtt tacgtccgtt agcaatcagg ggagaattgt
    40201 ctaaaccagt atcaccgttt tatatggaga tatcaacaat aggaaaattt gaaaaagaat
    40261 taggtcgagg aatggcttcg gcaccgatta cttttgggca tttataaaaa cgagttattc
    40321 atctcaatga attaaaaagt atagtcaatc tcataatgca ggctaggttc atttatttga
    40381 acagagtctg tattttttta ttctaaataa agaaacagat gtttctttat attgtatttt
    40441 atcctttaat taacacaaaa acagctagta taaactaata atgttaatta aaagtgtatt
    40501 ttatgaaact atcgttattt attaatgttg catttttgta agttaaaaat tggaaatata
    40561 gattgaaaaa atgaaagc
//
LOCUS       DAFEHS010000016        38339 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFEHS010000016
VERSION     DAFEHS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.016"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            162..392
                     /locus_tag="LP327_000923"
     CDS             162..392
                     /locus_tag="LP327_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBL1725060.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="LP327_000924"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="LP327_000924"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL1725061.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="LP327_000925"
     CDS             1803..1979
                     /locus_tag="LP327_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL1725062.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="LP327_000926"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="LP327_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBL1725063.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2594..3061
                     /locus_tag="LP327_000927"
     CDS             2594..3061
                     /locus_tag="LP327_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1725064.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3146..4030
                     /locus_tag="LP327_000928"
     CDS             3146..4030
                     /locus_tag="LP327_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL1725065.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4144..4380
                     /locus_tag="LP327_000929"
     CDS             4144..4380
                     /locus_tag="LP327_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBL1725066.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4468..4548
                     /locus_tag="LP327_000930"
     tRNA            4468..4548
                     /locus_tag="LP327_000930"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4502..4504,aa:Tyr,seq:gta)
     gene            4555..4626
                     /locus_tag="LP327_000931"
     tRNA            4555..4626
                     /locus_tag="LP327_000931"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4587..4589,aa:Gln,seq:ttg)
     gene            4751..4921
                     /locus_tag="LP327_000932"
     CDS             4751..4921
                     /locus_tag="LP327_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725067.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5141..5848
                     /locus_tag="LP327_000933"
     CDS             5141..5848
                     /locus_tag="LP327_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL1725068.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5908..6552)
                     /locus_tag="LP327_000934"
     CDS             complement(5908..6552)
                     /locus_tag="LP327_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBL1725069.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6679..6966
                     /locus_tag="LP327_000935"
     CDS             6679..6966
                     /locus_tag="LP327_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725070.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7074..7394
                     /locus_tag="LP327_000936"
     CDS             7074..7394
                     /locus_tag="LP327_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1725071.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7475..8788
                     /locus_tag="LP327_000937"
     CDS             7475..8788
                     /locus_tag="LP327_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL1725072.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8785..9504
                     /locus_tag="LP327_000938"
     CDS             8785..9504
                     /locus_tag="LP327_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBL1725073.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9632..10183
                     /gene="raiA"
                     /locus_tag="LP327_000939"
     CDS             9632..10183
                     /gene="raiA"
                     /locus_tag="LP327_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBL1725074.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10405..12939
                     /gene="secA"
                     /locus_tag="LP327_000940"
     CDS             10405..12939
                     /gene="secA"
                     /locus_tag="LP327_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBL1725075.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13065..14166
                     /gene="prfB"
                     /locus_tag="LP327_000941"
     CDS             join(13065..13136,13138..14166)
                     /gene="prfB"
                     /locus_tag="LP327_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBL1725076.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14271..14957
                     /gene="ftsE"
                     /locus_tag="LP327_000942"
     CDS             14271..14957
                     /gene="ftsE"
                     /locus_tag="LP327_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBL1725077.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14941..15834
                     /locus_tag="LP327_000943"
     CDS             14941..15834
                     /locus_tag="LP327_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725078.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15986..17434
                     /gene="cls"
                     /locus_tag="LP327_000944"
     CDS             15986..17434
                     /gene="cls"
                     /locus_tag="LP327_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL1725079.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17771..18628
                     /locus_tag="LP327_000945"
     CDS             17771..18628
                     /locus_tag="LP327_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBL1725080.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18743..19663
                     /gene="pstC"
                     /locus_tag="LP327_000946"
     CDS             18743..19663
                     /gene="pstC"
                     /locus_tag="LP327_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBL1725081.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19663..20547
                     /gene="pstA"
                     /locus_tag="LP327_000947"
     CDS             19663..20547
                     /gene="pstA"
                     /locus_tag="LP327_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBL1725082.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20562..21368
                     /gene="pstB"
                     /locus_tag="LP327_000948"
     CDS             20562..21368
                     /gene="pstB"
                     /locus_tag="LP327_000948"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL1725083.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21384..22142
                     /locus_tag="LP327_000949"
     CDS             21384..22142
                     /locus_tag="LP327_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL1725084.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22155..22832
                     /gene="phoU"
                     /locus_tag="LP327_000950"
     CDS             22155..22832
                     /gene="phoU"
                     /locus_tag="LP327_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBL1725085.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23070..24101
                     /locus_tag="LP327_000951"
     CDS             23070..24101
                     /locus_tag="LP327_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL1725086.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24098..24865
                     /locus_tag="LP327_000952"
     CDS             24098..24865
                     /locus_tag="LP327_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL1725087.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24965..26533
                     /gene="liaX"
                     /locus_tag="LP327_000953"
     CDS             24965..26533
                     /gene="liaX"
                     /locus_tag="LP327_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBL1725088.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26830..27150
                     /locus_tag="LP327_000954"
     CDS             26830..27150
                     /locus_tag="LP327_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBL1725089.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27150..27500
                     /locus_tag="LP327_000955"
     CDS             27150..27500
                     /locus_tag="LP327_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL1725090.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27667..28605
                     /locus_tag="LP327_000956"
     CDS             27667..28605
                     /locus_tag="LP327_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBL1725091.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28620..29450
                     /locus_tag="LP327_000957"
     CDS             28620..29450
                     /locus_tag="LP327_000957"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL1725092.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29494..30519
                     /locus_tag="LP327_000958"
     CDS             29494..30519
                     /locus_tag="LP327_000958"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL1725093.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30534..31472
                     /gene="galU"
                     /locus_tag="LP327_000959"
     CDS             30534..31472
                     /gene="galU"
                     /locus_tag="LP327_000959"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL1725094.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31597..32037
                     /locus_tag="LP327_000960"
     CDS             31597..32037
                     /locus_tag="LP327_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBL1725095.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32040..32642
                     /locus_tag="LP327_000961"
     CDS             32040..32642
                     /locus_tag="LP327_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL1725096.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32705..33808)
                     /locus_tag="LP327_000962"
     CDS             complement(32705..33808)
                     /locus_tag="LP327_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL1725097.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            34018..35019
                     /gene="ccpA"
                     /locus_tag="LP327_000963"
     CDS             34018..35019
                     /gene="ccpA"
                     /locus_tag="LP327_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBL1725098.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35374..35769
                     /locus_tag="LP327_000964"
     CDS             35374..35769
                     /locus_tag="LP327_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725099.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35769..36020
                     /locus_tag="LP327_000965"
     CDS             35769..36020
                     /locus_tag="LP327_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725100.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            36063..36458
                     /locus_tag="LP327_000966"
     CDS             36063..36458
                     /locus_tag="LP327_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725101.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36455..37711
                     /locus_tag="LP327_000967"
     CDS             36455..37711
                     /locus_tag="LP327_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBL1725102.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37723..38046
                     /locus_tag="LP327_000968"
     CDS             37723..38046
                     /locus_tag="LP327_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725103.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            38039..38305
                     /locus_tag="LP327_000969"
     CDS             38039..38305
                     /locus_tag="LP327_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725104.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12859 a         6515 c         8262 g        10703 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaacaatt tggaaaatag cttcttatat tttgcaaaat tgtgagttat
     2461 tgccgaagtg ttggattttg aacaccgtca aaaaaaggtt gtgaaaaata cgggtagcac
     2521 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2581 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2641 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2701 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2761 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2821 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2881 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2941 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     3001 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     3061 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3121 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3181 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3241 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3301 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3361 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3421 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3481 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3541 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3601 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3661 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3721 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3781 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3841 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3901 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3961 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     4021 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     4081 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4141 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4201 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4261 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4321 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4381 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4441 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4501 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4561 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4621 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4681 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4741 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4801 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4861 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4921 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4981 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     5041 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     5101 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5161 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5221 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5281 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5341 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5401 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5461 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5521 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5581 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5641 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5701 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5761 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5821 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5881 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5941 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     6001 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     6061 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6121 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6181 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6241 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6301 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6361 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6421 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6481 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6541 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6601 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6661 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6721 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6781 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6841 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6901 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6961 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     7021 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     7081 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7141 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7201 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7261 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7321 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7381 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7441 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7501 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7561 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7621 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7681 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7741 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7801 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7861 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7921 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7981 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     8041 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     8101 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8161 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8221 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8281 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8341 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8401 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8461 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8521 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8581 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8641 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8701 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8761 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8821 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8881 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8941 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     9001 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     9061 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9121 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9181 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9241 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9301 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9361 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9421 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9481 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9541 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9601 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9661 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9721 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9781 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9841 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9901 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9961 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
    10021 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
    10081 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10141 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10201 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    10261 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    10321 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    10381 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    10441 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    10501 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    10561 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    10621 gcaaaacgtg tattggggtt atatccacac cacgtacaat tgatgggtgg aatcgtgctt
    10681 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    10741 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    10801 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    10861 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    10921 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    10981 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    11041 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    11101 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    11161 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    11221 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    11281 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    11341 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    11401 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    11461 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    11521 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt
    11581 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    11641 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    11701 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    11761 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    11821 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    11881 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    11941 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    12001 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    12061 cgaatcaaat ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    12121 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    12181 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    12241 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    12301 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    12361 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact
    12421 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    12481 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    12541 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    12601 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    12661 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    12721 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    12781 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    12841 aaacagccaa tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg
    12901 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    12961 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    13021 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    13081 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13141 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13201 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13261 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13321 ttgcaggaag aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta
    13381 gatgaaaaaa tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac
    13441 aacgccatcg ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt
    13501 atgcttttac gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13561 gattatcaag caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13621 aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13681 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13741 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    13801 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    13861 cctacaggaa cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa
    13921 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    13981 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    14041 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    14101 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    14161 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    14221 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14281 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg
    14341 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    14401 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14461 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14521 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    14581 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc
    14641 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    14701 aacgtgtctc tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac
    14761 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    14821 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14881 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    14941 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    15001 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc
    15061 ggtgtattca tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac
    15121 gttgatgtct ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    15181 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    15241 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    15301 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    15361 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    15421 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    15481 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    15541 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    15601 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    15661 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    15721 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    15781 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    15841 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    15901 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    15961 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    16021 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    16081 tcaaacatgg gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16141 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16201 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16261 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16321 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16381 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16441 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16501 agggggaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16561 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16621 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16681 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16741 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16801 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16861 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16921 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16981 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    17041 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    17101 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17161 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17221 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17281 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17341 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17401 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17461 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17521 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17581 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17641 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    17701 ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag
    17761 gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt
    17821 atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc
    17881 tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt
    17941 catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt
    18001 cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt
    18061 agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt
    18121 aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag
    18181 tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac
    18241 ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca
    18301 agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt
    18361 tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac
    18421 agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag
    18481 agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca
    18541 agataacatc gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga
    18601 ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac
    18661 ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa
    18721 cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa
    18781 acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt
    18841 gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac
    18901 tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg
    18961 agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac
    19021 ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga
    19081 gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat
    19141 tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat
    19201 ttttggcggg actgggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt
    19261 gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga
    19321 agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc
    19381 agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc
    19441 cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggtc ttactactcc
    19501 tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt
    19561 tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa
    19621 tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca
    19681 gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca
    19741 gctttactac tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc
    19801 gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt
    19861 tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat
    19921 ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc
    19981 ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa
    20041 ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct
    20101 cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca
    20161 ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg
    20221 acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct
    20281 gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca
    20341 ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt
    20401 tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct
    20461 gccgtgttga ttattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga
    20521 ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga
    20581 atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc
    20641 tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa
    20701 ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt
    20761 tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag
    20821 gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg
    20881 tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga
    20941 aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac
    21001 aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg
    21061 gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc
    21121 tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac
    21181 ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag
    21241 ctcgtatcag cgactatact gcctttttct atttaggaaa agtaatggaa tatgacaaaa
    21301 ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt
    21361 ttggttaaga cggaggcctc aacatgacaa aagagattat ttcatctaaa gatcttcatc
    21421 tgtattatgg aaagaaagaa gcactaaaag ggatcgatct aagcttcaat caaggggaaa
    21481 tcactgcgat gatcggtcct tctggttgcg gaaaatccac ttatctgcgt tctttgaacc
    21541 ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag
    21601 acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc
    21661 agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa
    21721 aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt
    21781 ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc
    21841 agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg
    21901 atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag
    21961 aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta
    22021 tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga
    22081 agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat
    22141 aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc
    22201 agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca
    22261 tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca
    22321 tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa
    22381 ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg
    22441 atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg
    22501 aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc
    22561 tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa
    22621 gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc
    22681 ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg
    22741 gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg
    22801 agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt
    22861 aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga
    22921 acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat
    22981 gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc
    23041 ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat
    23101 tttttatttg tgtgcttact tcatgggtta ttcagcatca agccgcttta tatgaagtac
    23161 ctgtcatcga gatagaaaaa gtacgaataa aagaggatca tcaattgatt acaggaaaac
    23221 tgatgaacac ggatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc
    23281 aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg
    23341 aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca
    23401 ttggcgccat gttgcttatt ggtggaaaac agggtctatt tgctttagga agtctcatca
    23461 tcaatatcgt tttactttta cttttgttgc tcatctttac taaaacagaa gggatttctc
    23521 tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag
    23581 atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag
    23641 cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg
    23701 aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa
    23761 cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta
    23821 agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag
    23881 atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat
    23941 cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat
    24001 cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct
    24061 cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt
    24121 tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt
    24181 aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg
    24241 gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt
    24301 tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt
    24361 ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt ttacatcggt
    24421 agaactagaa gaattatctg gcttagataa gactttagcg attgatttta gattacttac
    24481 gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc
    24541 tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca
    24601 ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc
    24661 attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca
    24721 gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt
    24781 gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa
    24841 gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga
    24901 atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat
    24961 ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga
    25021 agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa
    25081 agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga
    25141 taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag
    25201 acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac
    25261 ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga
    25321 gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga
    25381 agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga
    25441 tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca
    25501 atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga
    25561 tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt
    25621 aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga
    25681 atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt
    25741 ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac
    25801 gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa
    25861 aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga
    25921 tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc
    25981 taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac
    26041 attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt
    26101 aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa
    26161 catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga
    26221 aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt
    26281 gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat
    26341 cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta
    26401 cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc
    26461 agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga
    26521 tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt
    26581 tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagt ctatctcttg
    26641 aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt
    26701 tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa
    26761 agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag
    26821 aggtgagaca tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt tttgacaggg
    26881 actttagcag gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat
    26941 gtattcctga gcttagttgc ttttggaagt ccaattattt tatacatttt attagctttg
    27001 attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa
    27061 aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa
    27121 atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata
    27181 cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt
    27241 ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt
    27301 tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca
    27361 atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt
    27421 cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg
    27481 aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt
    27541 agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg
    27601 gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta
    27661 gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc
    27721 gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca
    27781 ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc
    27841 agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga
    27901 agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc agctccagaa
    27961 gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact
    28021 tctcgtttat taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt
    28081 gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg
    28141 ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gccatcgatt gatcgcagac
    28201 gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc
    28261 ctggaacatc tgattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt
    28321 gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca
    28381 aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg
    28441 ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa
    28501 gtagccgcga tgaatttccg tgcacgtaca atgggctttg acgcaacgaa aacatttgaa
    28561 gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata
    28621 tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg
    28681 cattgatcat cgtatcaggt atcattatcg ctatgtggct tagcagcaga gaggctgttc
    28741 gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca
    28801 ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa
    28861 tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg
    28921 cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact tttttagata
    28981 tcgctgcacc aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc
    29041 acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat
    29101 tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat
    29161 cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt
    29221 tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta
    29281 ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat
    29341 tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa
    29401 atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc
    29461 gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga
    29521 ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg
    29581 atttggggac atcatgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt
    29641 tatttaccag atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca
    29701 gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca
    29761 aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta
    29821 gaacagggaa cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa
    29881 agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc aaaacatgac
    29941 atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg
    30001 ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg
    30061 gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc
    30121 gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta
    30181 gcaatgggtg ccaacccgct gacctttatc ggattaagcg gaataggcga tctagtcgtt
    30241 acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag
    30301 cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa
    30361 gcagcgatgg aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat
    30421 gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat
    30481 ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag
    30541 ttaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag
    30601 ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag
    30661 aagcgttgaa atcaggtatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa
    30721 tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga
    30781 ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat
    30841 ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac
    30901 catttgtcgt tatgctaggc gatgatataa tggaagacga agtaccattg acacagcaat
    30961 tgatcgatga ctatgagcgt acacacgctt ctacgattgc cgttatgcaa gttcctcacg
    31021 atgaaacttc aaaatacgga atcatcaacc caggggaagt actggaagat gggttataca
    31081 atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta
    31141 ttgggcgtta cttattgact ccagaaatct ttgatgtatt atcagaacaa caaccaggtg
    31201 ctggaaatga gatccaacta actgacgcaa tcgatacgtt gaataaaaca caacgcgtat
    31261 ttgcccgtaa attcacaggt aaacgttatg acgttggtga caaatttggt tttatgaaaa
    31321 caagtattga atatggtctg acacatccag aagtaggggc acctttgcgt gaatacatca
    31381 tgtctttagg taaaacatta gaagcagaag gaagcaagat caatcataat ccaaaaggaa
    31441 taacagaaat caaagaagaa aagaaaaaat aatctttttt tctaacgagc ttcagctgct
    31501 aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat
    31561 ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga
    31621 ttgatcgcag caatcgcctt tgcgatactt gtcgttttca tcgttcgcgt attgttgcaa
    31681 gtttccaaaa ctttagaaaa agtagaccag actgtcgcag aagcaaatac gacaattgag
    31741 atcgtcacac aagacgtaga tcttttatct agacaagtag aaggcttgct agtcaaaagt
    31801 aatgagctgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca
    31861 gttgctgatt taagtgaaag tgtatcggaa cttaatcatt ctagtaaaaa catcgtgacg
    31921 aaagtcggcg gaatcggaaa aacgacagct aaagcgactg ttgccggaaa agtcggtgga
    31981 acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataagaa
    32041 tggctaaaaa aggtggattt ttattaggtg cagtagttgg aggtacagcc gcagcaatcg
    32101 ccgcattatt gttagcaccc aaatctggta aagaacttcg ggatgaattg gctgctcaag
    32161 cagatgattt gatggacatg gcttcagatt acacagatat ggcaaaagaa aaatcatctg
    32221 agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc tcaacaagca ggggatctag
    32281 caggcaatgt taagcagaag gtaagtgaat cagcagagga agcaggaagt gaatcagatg
    32341 atatcttgtt aaacctcaaa gaacaatcag ctgatttatc taatcgtttc aaaaaaacag
    32401 cagaagatct gaaaaagcca gcagacgaat taggtagtat tgctaaagat acttcagaag
    32461 atatcatgat cgatgtaaaa gatgctgcag ataaagcagg agagacggtc aatgaaggaa
    32521 tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat gttgaaaatg gcaaaagaag
    32581 atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac agattcgact accaatcact
    32641 aagattactc atttctagaa taaaaaaggt gagagcggac cagctctcac cttttttatt
    32701 ttcattactc taaaacaagt aattcttttg gtgtagtcgt gaatggcaca caaccatcac
    32761 ttgtcacata cacacaatct tcgatacgta cccctacttt ttcaggaata tagattcctg
    32821 gttcaagaga gaagcacatc ccttcttcaa tcactaagtc gtttccttct acaagagaag
    32881 ggaattcgtg gacggttgtt ccgattccgt gacctaaacg gtgattgaaa tattcgccgt
    32941 agccatagct gttgatcacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg
    33001 ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt gatgttatag attttttctt
    33061 ggaactcagt tggtttttga taagcaactg tacgagtagc atcactgcaa tagccattcc
    33121 aaataacgcc aagatcgaat aacactagtt catttggtga aaccttcgtg cttcctggtg
    33181 taccatgagg gcttgcagca tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg
    33241 aaacaccttg tttttttaat tggtattcga tttctgcgat aattgcttgt tcagttacgc
    33301 cttcttttac tgttttgaag ccaatttcaa aggcaacatc ggcccattta ccagcttcga
    33361 tcagtttgtc tttttcttct accgttttga tcagttgcag acgctggata atagaagtta
    33421 gatcggtgta agaatctgta cctgtcaaga tatcagagat agcatcgaag cgatcgacag
    33481 tcagagcatt cttttcaata cctacattac caggttgccc atagcgtgcg cgaagctctt
    33541 ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc acgtacatcg tacgcccagc
    33601 tgctgttttg agcatcttca acttccagag caggagtgaa aaggaaagga tcttgttcca
    33661 gagggataaa aagagctagt acacgctcgt gaggatcact ttcatatccg gaaaagtaag
    33721 cgatgtggcc agggtcgctg atataagcca attctacttg gtgttgtgcc atccattgac
    33781 gtagttcatt gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat
    33841 tcaataattg taccactgat tgattaaaag accaacgttc ttaaaactga aaaaagtaaa
    33901 aattttcatg aaaacagaga taaaacggtt gcaattgttg agattatttg atatcctatt
    33961 ttagaaatgt aagacgtatt ttcagaaaac atataaaata tgaataggag aacacaaatg
    34021 gaaaagcaaa caatcactat ctatgatgta gcgagagagg caaatgtttc gatggctact
    34081 gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag caacaagaaa aaaagtcttg
    34141 gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg cacgtggctt ggcaagtaag
    34201 aaaacaacaa cagttggggt aatcattcct gatgtcagca atatgttctt tgcctcttta
    34261 gcacgtggta tcgacgatgt ggcaacaatg tacaaataca atatcatctt agcaaactca
    34321 gatggcaacg atcaaaaaga agtcaatgta ttgaataatt tattggctaa gcaagtggac
    34381 ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc gtggagaatt ttcacgttca
    34441 aaaacgcctg ttgtactagc tggttccatt gatccagatg aacaagtagg tagcgtaaac
    34501 attgattatg tgtcagcaac aaaagatgca gtaaataaat tagcaaaaag cggcaataaa
    34561 aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca atggtcaaaa ccgtctaaaa
    34621 ggctataaag aagcattaag cgaaaacaat ctaccatata gcgaaggatt gatttttgag
    34681 gcacaataca actttaaaga tggtctgtct ctagttgacc ggatccataa tagcggtgca
    34741 acagctgtct atgtttcaga cgatgaatta gccattggta tcttggatgg tttattagac
    34801 cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa gcaacaattc tttactgaca
    34861 gaagtagctc gtcctcgttt gacaagtatc acacaaccat tatacgattt aggagcggta
    34921 tccatgcgcc tattgacgaa aatgatgaat aaagaagaag tcgaagaaaa aacgattatc
    34981 ttgccttatg gaatcgaaga aaaaggatca actaaataat aaaagatcaa tcatttaaag
    35041 gttgttccaa aagtggtcaa cttctggaac aacgtttata cgagaaaggg ggttatgaca
    35101 aaattttgtc ataacccctt tttttttgtg actttaaaaa aagagaaaat ggcggaaaca
    35161 aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga tgctacattt tgttcactac
    35221 tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa agtagaaaag tagataagta
    35281 actctaaaaa atccaaaaaa atccaggctc gattgcgtac ttatcttaaa ggagaaacaa
    35341 tccatcatat ataacccgaa aggagacttc tatttgccta agaaaagacg aaagaccata
    35401 gtcatcattt tgaccattct tactatagga atcggaggga aattttacat ggacaaaaga
    35461 gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac agtccgtcaa agttctgaaa
    35521 aatacctttg cggatatcaa ggaagtgaag attgaaaaat ctgctcgaaa tgaaatgacg
    35581 ggttcatacc ggatagttat tctaatgact aataaacaag atcaatcgat ttatttttct
    35641 tatagttttt ggaaagaaag aaatgagata ggaagttacg gaatagttga tgaaaaaaag
    35701 caaaaagaag gaaatacgct caataaagtt aaagtaactt actctaatgg aaatgaggaa
    35761 agtatttaat gattactgac aaacaatata gtgaattaag tgatgctgtt tactggctag
    35821 atcctaaaca tagagactat gtacctgaaa tgcaagaaaa tttaagtttt aaaattaacg
    35881 gcactatgta taaaatccta aaaatcaaaa actccttcga cggcatgcag gcgatggctg
    35941 tagcgccaat cgtccatagc aaattggaga aaaatttcaa aaataaaaaa attcctgcaa
    36001 actttcgcgt acttaaataa tgaaggtgtc aatacttaca taaaaagaaa ggaggcacgt
    36061 tcttgtcaag gaaaataaga aaactcagct tagccatttt attaatcgtc atattaggga
    36121 tcggagggaa agtctatatg gacaaaagag aagcacaaag gcagaaagat ttactcgcag
    36181 tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc ggatatcagg gaagtgaagg
    36241 tagaagaatt taaaaaaaac cctgttaccg gaagttatgg tgcacttgtg acaatgacaa
    36301 ataatgaagg gaaatctgtt tattttgatt atagtttttg gaaggagaga aatgaacttg
    36361 gtggttttgg cattgagaac agagcggtac aaaagacagg agtcactata aaaagagtaa
    36421 aagtaatttt ttctaacgga caggaggagt tagtatgaca ataaacgata agcattacaa
    36481 tgatattagt gagaaggttt attggctaga tcccaaatat ccccgctaca atgagggata
    36541 caaaaagaac tctgttaaag aatttgctgg tatggaattt caaatcctac aaatcaaaga
    36601 ctcattggac ggcatgcagg cgatgtctgt agcgccaatc aagaacggta aagtggatct
    36661 ttctcaagta gtgattgctt ttgcaggaac gaacttaagt gattggaaag atttggaaac
    36721 ggatgcaaga agtatcatga atctctacgg gaatagacca ggagcaagtt tttctgaaaa
    36781 agcaacaaca aacaagattt tatccgttag tggattgaaa acagcggggc aaatcaattc
    36841 agcgattgca tttgctaatg aagtaaaaaa ggaatatgga gaagctgcta tttctactac
    36901 cggccattca ttaggtggat tcttagcatt atatgtagca gcagagaaac aatggaaaaa
    36961 tgtcggcttc aacgggccgg acccgtatga tctcttatca ccagaagcaa aaaaatgggt
    37021 ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac gccgatttta tcggtaattt
    37081 tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg gaaacagcat tcttgaatct
    37141 gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa aatggaaagc tgattattcc
    37201 tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa gaaaaccaag cgacccagtt
    37261 gttttcctat cagttgcaag aactgaacaa catacgtaaa aaactacgat taagtggcgg
    37321 ccagctgaca gttaatgaac aaatttactt agatgacaca caagctaggt tagtagtgga
    37381 aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt atccgcatca atcaaagagg
    37441 aatttttgag ctagagaaaa aatggcaaga aggcttgcgg caagcacgtg ttgtagcccc
    37501 ttcgttgact gaacaggaaa tcatagaagc attagctagt gtgggcgcca caaaagaaaa
    37561 cttagtcgat tctgcgaaag aagtctatct gctacgaatc aataaggcaa gacgtatggg
    37621 ggaggccttt gatacattgg cgaaggagat ccaagacaaa atcaatgaaa tcgtggcacg
    37681 agatcgagaa ttagcacagc aattcaatta aaggagaagt agatgaaact agaagaattg
    37741 gaaagagatt atcgaagtga acgaagacaa ttagaagctc aagaagatga attgatccac
    37801 tttaagaatc aaggattacg attgatggag caagaggagc agcgactgca tcatgtatta
    37861 aagaacttag ctttagataa tgagcctctg tttcaagcaa gacgaagtta ttcgaattat
    37921 caggagcatt atcaacaagc cctacaagta gaacgacgaa aactctatca gcagcaagat
    37981 gaactagaac gttcctatcg tcaaaaaatc agacaaataa ctatggagaa atcatcttat
    38041 gaatgaattt ttaatgcaat tactaaaaga attagagaaa aatctacatc gatcaaatgt
    38101 acgttcagtt ggtatgggga tatctcagca aatttgggca gaaaaacgat tgacagatca
    38161 aaactaccaa atgactgcag aagaagctgc tcaagaattt tctaaccgac atgtacactt
    38221 gaaaggatca gtaaaagggc aatttgcaga aaaactaact gaattattcc aaagacaaga
    38281 aaagaaatta aaacagtttc gttagtaaag ttatatagaa aaattttgtg aaaatggtt
//
LOCUS       DAFEHS010000017        37670 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFEHS010000017
VERSION     DAFEHS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.017"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            142..1209
                     /gene="rlmN"
                     /locus_tag="LP327_000970"
     CDS             142..1209
                     /gene="rlmN"
                     /locus_tag="LP327_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL1725105.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1402..2505)
                     /locus_tag="LP327_000971"
     CDS             complement(1402..2505)
                     /locus_tag="LP327_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL1725106.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3058..3606
                     /gene="aac(6')"
                     /locus_tag="LP327_000972"
     CDS             3058..3606
                     /gene="aac(6')"
                     /locus_tag="LP327_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL1725107.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            4152..5813
                     /locus_tag="LP327_000973"
     CDS             4152..5813
                     /locus_tag="LP327_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL1725108.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(5883..6230)
                     /locus_tag="LP327_000974"
     CDS             complement(5883..6230)
                     /locus_tag="LP327_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBL1725109.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            6314..7255
                     /locus_tag="LP327_000975"
     CDS             6314..7255
                     /locus_tag="LP327_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725110.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            7256..8299
                     /locus_tag="LP327_000976"
     CDS             7256..8299
                     /locus_tag="LP327_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725111.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            8305..9375
                     /locus_tag="LP327_000977"
     CDS             8305..9375
                     /locus_tag="LP327_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725112.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            9365..10318
                     /locus_tag="LP327_000978"
     CDS             9365..10318
                     /locus_tag="LP327_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1725113.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            10330..11340
                     /locus_tag="LP327_000979"
     CDS             10330..11340
                     /locus_tag="LP327_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL1725114.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(11625..12446)
                     /locus_tag="LP327_000980"
     CDS             complement(11625..12446)
                     /locus_tag="LP327_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL1725115.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(12543..12821)
                     /locus_tag="LP327_000981"
     CDS             complement(12543..12821)
                     /locus_tag="LP327_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725116.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            13089..15098
                     /gene="metG"
                     /locus_tag="LP327_000982"
     CDS             13089..15098
                     /gene="metG"
                     /locus_tag="LP327_000982"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBL1725117.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(15577..16164)
                     /locus_tag="LP327_000983"
     CDS             complement(15577..16164)
                     /locus_tag="LP327_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725118.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            16339..17661
                     /locus_tag="LP327_000984"
     CDS             16339..17661
                     /locus_tag="LP327_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1725119.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            17816..18592
                     /locus_tag="LP327_000985"
     CDS             17816..18592
                     /locus_tag="LP327_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL1725120.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            18585..19154
                     /gene="rnmV"
                     /locus_tag="LP327_000986"
     CDS             18585..19154
                     /gene="rnmV"
                     /locus_tag="LP327_000986"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBL1725121.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            19147..20031
                     /gene="rsmA"
                     /locus_tag="LP327_000987"
     CDS             19147..20031
                     /gene="rsmA"
                     /locus_tag="LP327_000987"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBL1725122.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            20482..21111
                     /locus_tag="LP327_000988"
     CDS             20482..21111
                     /locus_tag="LP327_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725123.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(21235..22344)
                     /gene="ugpC"
                     /locus_tag="LP327_000989"
     CDS             complement(21235..22344)
                     /gene="ugpC"
                     /locus_tag="LP327_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBL1725124.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            22553..22975
                     /gene="mgsA"
                     /locus_tag="LP327_000990"
     CDS             22553..22975
                     /gene="mgsA"
                     /locus_tag="LP327_000990"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBL1725125.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            23298..23834
                     /locus_tag="LP327_000991"
     CDS             23298..23834
                     /locus_tag="LP327_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL1725126.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(23874..25637)
                     /locus_tag="LP327_000992"
     CDS             complement(23874..25637)
                     /locus_tag="LP327_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725127.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(25640..27355)
                     /locus_tag="LP327_000993"
     CDS             complement(25640..27355)
                     /locus_tag="LP327_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725128.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(28134..28358)
                     /locus_tag="LP327_000994"
     CDS             complement(28134..28358)
                     /locus_tag="LP327_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725129.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            28549..31326
                     /locus_tag="LP327_000995"
     CDS             28549..31326
                     /locus_tag="LP327_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL1725130.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            31407..31826
                     /locus_tag="LP327_000996"
     CDS             31407..31826
                     /locus_tag="LP327_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBL1725131.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            31838..32308
                     /locus_tag="LP327_000997"
     CDS             31838..32308
                     /locus_tag="LP327_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1725132.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            32325..33104
                     /locus_tag="LP327_000998"
     CDS             32325..33104
                     /locus_tag="LP327_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL1725133.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            33091..33912
                     /locus_tag="LP327_000999"
     CDS             33091..33912
                     /locus_tag="LP327_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL1725134.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            33929..34978
                     /locus_tag="LP327_001000"
     CDS             33929..34978
                     /locus_tag="LP327_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL1725135.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            34991..35995
                     /locus_tag="LP327_001001"
     CDS             34991..35995
                     /locus_tag="LP327_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL1725136.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            36628..37647
                     /locus_tag="LP327_001002"
     CDS             36628..37647
                     /locus_tag="LP327_001002"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL1725137.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        12298 a         6641 c         7682 g        11049 t
ORIGIN      
        1 ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa gttctattcg
       61 atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta gcgaaacttt
      121 gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac gaatgaagaa
      181 ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca agtttgggaa
      241 tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc gaaacaattg
      301 atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt cgtccaagaa
      361 gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat gattgagact
      421 gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt aggatgtaat
      481 atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct gactgctgga
      541 gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa tcttggcgaa
      601 cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta cgacaatgta
      661 atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc tcggcacatt
      721 acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa cggcttgcaa
      781 gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg
      841 atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta tttagagaaa
      901 acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga tagaccagag
      961 catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata tgtcaacttg
     1021 attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa agctgatgta
     1081 ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat
     1141 ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt
     1201 gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag ttttcttaat
     1261 taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta taaagctttt
     1321 tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga cagcctgctt
     1381 gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc tttcttgtgt
     1441 agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg tgatttcata
     1501 aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt ctaaatcggt
     1561 cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt tcataaacac
     1621 atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca gtacaccata
     1681 actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg taccgtaata
     1741 gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa ccaatccggt
     1801 agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat cgtcggcact
     1861 atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt ttccgatttg
     1921 gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag tagattcgac
     1981 ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg cttcggcaat
     2041 aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat
     2101 tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat
     2161 agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta cagtatcgca
     2221 agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt catgctggat
     2281 caactgttca atatttccat aagttgaatt acccccgtac cagagactgg cgacaacctc
     2341 tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg cagtcttccc
     2401 tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt tttcatacgc
     2461 atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca tttccttctt
     2521 tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg ctgtagatgg
     2581 agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac accttgaaat
     2641 catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac aataatgaat
     2701 ttttgttcct cttagtattt ctaccgattc cctttgcttt tttgtaatta tctaaaaatt
     2761 ctaaaaatta agattattat atgaaaacag gttttatttg tcaattcata attggttttt
     2821 tttaaagcaa aagataatct atatcatgat gaacaaacag agttttttcc tccaattgat
     2881 ggctttcctg atctttccta ttttgaataa agtgtttctg tataattttt ttattttata
     2941 agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag
     3001 aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg
     3061 ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg
     3121 agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg
     3181 aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt
     3241 gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc
     3301 cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc
     3361 agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt
     3421 caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa
     3481 catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca
     3541 aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct
     3601 caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac
     3661 gaaaattact tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg
     3721 aacaccgtgt accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa gaaaaatttt
     3781 gtgaaaatgg ctttctacat ttttgcataa atttggctta ataccgagaa actggctttt
     3841 gaacaccgtt tattcagata agggactgtg acaagacttt tgtcacagtt tctttttgtt
     3901 tattcttttt tatctttgta tggcaataat agacaatata acgaaaatta tttaatggct
     3961 tttggaagac catggaaaac cttgtacaaa gctatttttt ctgaaaaatt ccttgaaacc
     4021 tcacgaaaaa atcattattt tatgaaaaag acaaaaaaaa gtgttgattt tttaataatt
     4081 attcattaaa ctcttaaaca acttcagaaa atattaatta ttctgaaaat tccaataaag
     4141 gggtacgtaa aatgaaaaag aaatttacat ttggtttggt tgcggtatgc ggccttgtac
     4201 ttgctggatg ttatggcggc agttcagcca caagcagtag ttcagcaaaa agcggttcag
     4261 cagatggagg aggcgtattt aacttagtcg tgcctcaaga aatgcctaca gctgacttat
     4321 cagttgcaac agatacaatc agttttacag cattgaataa cgtttatgaa ggaatttacc
     4381 gtcttgatga agacagcaaa ccacagccgg caggagctag cgagttggca gaagtcagtg
     4441 aagacggatt aacttacaaa atcaaattac gagaagacgc aaaatggtca aatggtgatc
     4501 ctgttacagc agcggattac gtttatggct ggcaacgaac agtagaccca gctacagctt
     4561 ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga agacattaca gctggaaaga
     4621 aagataaatc agaactaggg atcaaagcag ttggtgacta tgaattagaa atcaaattga
     4681 cgaaacaaac accatatttc caatatctgc ttgctttccc atcatttttc ccacaaaaac
     4741 aatccgtagt ggaagaaaat ggggatgcct atgcatctgc aagtgataaa gctgtttata
     4801 atggaccatt cacattagaa gattttgatg gaccaggaac agatacagaa tggacttata
     4861 agaaaaatga tcattactgg gacaaagata cagtaaaact ttctgaaatc aaagtcagtg
     4921 tagtaaaaga atcttctact gcattgaact tattcaaaga tggccaagca gatgacgtga
     4981 tcttgtctgg tgagttagca cagcaaaacg caaacgatcc agcttatact tctgttaaag
     5041 aagcgcggac aagctatatc gaattcaatc aacgtgaaaa gaattcacca ttcaacaatg
     5101 tgaacctaag aaaagctatt tcttattcta tcgatcgtga agctttagta aaacaagtac
     5161 taggtgacgg atcagttgtc tctacaggat tgatcccatc agatatggta aaaagtccaa
     5221 caacgaacga agactttgca aaagaagctg gaaaattagt ttcttatgac aaagataaag
     5281 caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt tgattcttta gaatttgatt
     5341 tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga atatgttcaa aactcaatcc
     5401 aagaaaactt ggatggcgta acagtcaaac caacacctgt tccattctct gtacgtcttg
     5461 accgctcaac ttcgggtgat tttgacgcag tactaggcgg atgggctgct gactatgcag
     5521 acccaagcag ctttactgat ttgtttgtca caggtaattc atacaaccgt ggtcgctgga
     5581 gcaatgcaga atatgacgaa gcagttaaag cagcttcatc aacagacgct ggcgatgaac
     5641 aagcccgttg ggatgatttc caaaaagcaa tcaagatcat ctctgatgat atgggtgtcg
     5701 ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga aaaaatcaaa ggtatcgttc
     5761 accatgcggc aggcgcttca tgggattaca aatggacata cgttgaagaa taatcgttaa
     5821 taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc gcaacctcgt tctcatattc
     5881 acttatccta acaaagaaaa aagcatgaaa atagcagaaa gagcaattag tattccggca
     5941 atgatcagcc aaaagctgta cataccgtag ctggcagacg agagcgagga aaattctggt
     6001 tttttctttc ggttccttgt tcgtgcaaga tggaccgatc gctgaaaatg atcgaagaaa
     6061 cccgaagaaa agacccataa aaaaccgccg ataatcagaa acggcaatga ccataagaat
     6121 agatcattag ataattgaat gagtgtaaag gaatggttga tgagattttt tattaaaata
     6181 accagaatca gtagaaaagc tactgcgtag gggatagatt tttttttcat ttgattgctc
     6241 ctttattttt ggttcattat ctaataatgt agcgaaacat ctgtcagaag tcaaaagcta
     6301 gggggaaaca ttagtgaata gttatatcaa atatgtatta aaacgtgtct ttttcatgat
     6361 catcacgctt tggttaattg caacaatcac ctttttcttg atgcaattgc tgccgggcac
     6421 gccatatacg aatcaggaac gtttgagtcc tgaaacaatc gagatgctaa ataaacaagt
     6481 cggtttggat aaaccagtta tcgtacaata tgggatttat ctttctaatt tgcttcaagg
     6541 agattttgga atttccttcc aatttaaaaa ccagccagtc gcacatttgt tagctggaag
     6601 aatcggacca tccttgcagt tagggttaca ggcaattatc tttggaacag tcctagggac
     6661 gattttagga acgatctctg ccatgaagca aaatacatgg gcagatacat cttctacact
     6721 cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt gctgttttat tacaatatat
     6781 ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg gatggctttg tgtacacaat
     6841 cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac tctgctcggt ttatccgtac
     6901 ggaaatggtg gaagtactgc atagtgatta tgtggaatta gcaaaagcaa aaggattgag
     6961 ccgttgggaa atcgccttta aacatggtct tcgaaatagt ttgattccat tgatgacttt
     7021 attaggaccg ttagctgtag cattgatgac cggatcactg gtggtagaaa atatctttgc
     7081 tattcctggt atcggggaac aattcgttaa gtcaatcaca acaaatgatt atcctacgat
     7141 catggctgta acgatccttt actcatttat gctgattttt gttattttga tcgtggatct
     7201 attgtacggt ctagttgatc caagaattcg tgtttcagaa gggagccgag gataaatgga
     7261 aatgatacca aagcgttatg aaacagttgc agatatccct gcggatgaat ttcttccatt
     7321 acaaaaaaat accgaagaag aacgtgaaca gattactgca ccttctttga attttatcca
     7381 agattcatgg cgccgtttaa agaaaaacaa agctgccgtt atttcaatgg ttttactatt
     7441 ggtcatcatt ttgatctctg tgatcacaat ctttgtttct cctcatgatc caactgccca
     7501 aaacgtggct tatatcaatt tgcctcctcg tattccggga atcaatatcg atggattgaa
     7561 tggaaaagca atggttggtg gtgaacttgt tgataaatat gctcaagcaa acgtcccttc
     7621 aaacgtaaat ttctttttag gaacagacgg tttgggacgt gatgtattaa gccgcttatt
     7681 tatgggaatg agaatctcgt tgctgatcgc atttattgct gcattactag atgtaacgat
     7741 tggtgtagct tacgggttgg tttctggttt gttaggcgga cgtgtcgata acgtgatgca
     7801 acgttttttg gaaatcttat caggaattcc aaacctcgtt gtcatgattt tgatgttggt
     7861 cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg gccatcacaa actggatacc
     7921 aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa gatcaagaat atgttttagc
     7981 tggattgaca ttaggtgaat caagaatgaa aattgcattc aaacatattt tgccgaatat
     8041 ctctagtgtc attattattc aaatgatgtt tagtattcca tctgctattt tcttcgaagc
     8101 ctttttaagc tttatcggtt taggtcttac gcctccgtca gcttcattag gaacgatgtt
     8161 gagcgaaggg tacaagacat tcctgtatct gccttatcta ttatggattc cggcagtaac
     8221 tttatcggtt atcatgattg gctttaactt acttgctgat ggattgcgtg atgcctttga
     8281 tcctaaaatg aaagagtgaa gacgatgacg aaaattttag aagtgaaaga cttagaaatc
     8341 tcttttgata cgtatgctgg aaaggtacga gcgattcgcg gtgtcaattt tcatttgaat
     8401 aaaggagaaa cattggcgat cgttggggaa tctggaagtg gaaaatcagt gacgaccaga
     8461 agcatcatgc gcttgctgtc aagcaacgcc aatatagatg ctggtcaaat cctctttaaa
     8521 ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa aaatccgtgg taaagaaata
     8581 gcgatgattt tccaagatcc aatgacctct ttagatccga cgatgcccat tgggaagcaa
     8641 gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa aagagggaca aaaagctgca
     8701 ttagaattgc ttaaattagt tggaataccc gaagctgaga aacgtatcaa cagttatccg
     8761 catcagttct caggaggtca gcgtcaacgg atcgtgatcg cgattgcact ggtatgttat
     8821 ccggaaatcc tgattgctga tgaacctaca acagcgcttg atgtgactat tcaagcacag
     8881 attttggagc tgctgaaaga aattcagaca aaaatcgata catcaattat ctttatcact
     8941 catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg ctgtcatgta tggaggaaga
     9001 atcgttgaag ttggaacatc ggaagaaatt ttctacaacc cgcaacatcc ctatacttgg
     9061 ggactgcttg gttcaatgcc gacattagat agtgcgaacg atcgattgta tgctattcct
     9121 ggttctccac cggatttatt gaacccgcca aaaggagacg cattctatcc aagaaatgaa
     9181 ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt ttgaactatc aaaaacacac
     9241 aaggcagcaa cttggctact tgctccgcag gcacccaaag tgacaccgcc agaagaaatc
     9301 caacgtcgct gggctatctt taaagagaaa caaaaacata cttacggtca aggaggaatt
     9361 gctgatgcca aataaactac ttgaagttaa aggattgaaa cagtatttca atgtaggaaa
     9421 aaagaatgaa gtacatgctg taaatgacat cagctttcac atttatgaag gagaaacatt
     9481 cggtttagtt ggagaatctg gaagcggaaa atccactact ggccgtacga tcattcgctt
     9541 gaatgaacct actgatggtg agatattatt cgacggtcaa gatataacga agatcaaaga
     9601 taaaaaaggt ttgacgaaat tccggcatga tgttcagatg atctttcaag atccatacgc
     9661 ttcgttgaat cctcgtatga aagtcagaga tatcattgcc gaaggaattg atgttaatgg
     9721 actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat gatctattga gaactgttgg
     9781 attgaatcca agccacggta ctcgttatcc tcatgaattt tcaggtgggc aacgccagcg
     9841 tattggtatt gcacgagcat tggcagtaaa accgcgtttt atcatttgtg atgaaccaat
     9901 ttctgcacta gatgtgtcga tccaagccca agtggtcaat ttgttgcaag atctccaaaa
     9961 agaacatcaa ttgacctatc tatttattgc acatgatttg tcgatggtta aacatattag
    10021 tgaccgaatc ggtgtaatgc ataacggctt gttattagag atgggaacaa gtgatgaaat
    10081 ctataatcac ggtgttcatc cttatacaga aagtttgtta tctgctattc ctctaccaga
    10141 tccagaccat gagagaaaac gcagaagaat caaatatcaa cctgaaccag atgacggaca
    10201 agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc tatgcaactg agcaggaagt
    10261 cccatattac gcaaaaaaac taaaacgaca aaaggaatct cttctggttg ctaactagga
    10321 gggacataga tgctaaaagc ttttaaattc cgtatgtatc cgacagaaga acaaaaacag
    10381 caattgattc gtacttttgg atgtgctcga tttacctata accatttgct gaaaaagcgg
    10441 cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc tcacacccgc tacattaaaa
    10501 aaagaatacc cattcttaaa agaagtagac agtcttgctt tagcaaatgc acagcttaat
    10561 ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca gcttcccaaa gcttaaaacg
    10621 aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa cccgtacgat ctatctggaa
    10681 aatggctact taaagttacc aaaacaaaaa gaactaatca agataaatca gcatcggcca
    10741 gttgaaggat cgattcgctc cgctacgatt tctgctcgat ataatgaaga attttatgtc
    10801 gcactacttt gtgatgtttc gccagtaaaa aaagaatctt tagctaaatg gatcgggatc
    10861 gcctatcatc cgaagacatt gatccaaaca tctcgacctc tagaagtaac tttaccaaaa
    10921 tttcaccaga cagaagaaaa gctgcaacat gcacagcgga aattgaatgt gaaagtccgc
    10981 tccgctcacc atagaaagat caggttagat caggcaagca attatcagaa acaaaagaga
    11041 aaagtaatgg atctttatct gaaacaaaag aaccagcgag aagattatct tgaacaatta
    11101 tctgggaaat tggtcaaaca atatgactac ttatttgtag aatcttttcc aaaagaagag
    11161 gcacatgctg atttttctat tcacgactgg cataaattaa taacaaaatt gcgttataag
    11221 tcccaatggt ataataaaaa atttcttttg atcaataccg acggagcaga agaaagcaat
    11281 tccgtaagaa agagccaagt agtagaaaaa atgggcagac attcggttat caagggataa
    11341 aaaatgaatg aaactgatag gagcgttagg gttagccttg ttaataaaaa gtcttaatga
    11401 cttttgtttc taggaatttt tcttttgaag aaaagctcac taattatgac aaaccaataa
    11461 aacaatagca gaaaaagttc gtacgagctt tttctgctat tgttaatttt ttattcagaa
    11521 agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa ctcacaaaga gttgtttttg
    11581 cgttttttct tgaatgatta ggtctgaatc ttttgctcac aaccttattc atggtgcccc
    11641 ggcttgagct gatagatttt tgaaggacgg ccgattccta caggacgttc accgatctca
    11701 tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat cgatcgtttt ataatcgatc
    11761 cctaagaatt tagcaaatac ttttcgagct tccgtaatcg tgaaatcttt acctaaaact
    11821 tgtagaactt gcggctcgtg ttccatttta ttgactacac ggttgaatgc tttcaagatg
    11881 atttgactat ggtcaaaagc caaagtatct ttgccagaag actctccagt gactaaatca
    11941 agcgagatag ccacttctcc gctagataaa tggatcttgt tactatgacg ttctagtgtg
    12001 aaccagcgta cttccttggc atcatctccg gcgattagcg gttcttctcc aataaaggct
    12061 aagtagctga ctgtgacgac ccagcctcgc ggatctcgat taggcgtact gaatgtatgc
    12121 agttgctcaa tattttcttt tgaaataacc acaccagttt cttcctttgt ttctcttaag
    12181 acgctttctc cagttgactc atccttttgg acaaagccac caggcaatgc ccatgaattt
    12241 cgataaggat gcccttttct ttggatcaac aaaactttca gctgatcttc ttctttgtta
    12301 tagcacatca gtacgatatc gacagtcaaa gagggcttgt catattctgg acgttcctgt
    12361 ttttgatacc agttaagaaa ttcttcttca cttgctttat gttcataata taatttttct
    12421 tcttctttag aagcaaaacg gttcatctga ccacctccat tgacagtaaa agtacttttt
    12481 accttattgt agcagactct gaaaaaaaac caaggattta cttgaaaaat ctaggttatc
    12541 atttaaatat gaaaaataaa tggaagtatg agattgaata aaaagaaaag cagataaaac
    12601 ggcaatactt tataccgctc ttcacgaatg gcaaagaagc cacaaataac gatatatacg
    12661 tcaatgataa aacgcaaaat ccaagctgct gtcataaaac tgttcaaatg cccatggagg
    12721 ttaaaataac catacatacg aatataaaaa tagctgaata ttaaatgtgc taatgaaaga
    12781 aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca tgattgttcc agtcccttac
    12841 taagatagat agtctttatt ataccacgtg tgaggggaga aataaaaagc cgttttttag
    12901 tagatatgaa acaaaacaaa aacccgcatt tattcaacct attcatcctt ttaaaggata
    12961 gattgaacgt agatagaagt cgtttatcca gtctttttca ttttgaaaaa aagctaacag
    13021 catgcttttt ttatggtata ctagcaagga aacgaattcg catcatgcga taaaggagga
    13081 agagccccgt ggcagaaaag gaaacttttt atatcactac gccaatctat tatccaagcg
    13141 gcaaacttca tatcggcaac tcgtatacta cgattgcatg tgacgcaatt gctcgttata
    13201 aacggttgat gggctttgat gtgttttatc tcactggagt ggatgaacat ggtcaaaaaa
    13261 tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga atatgtagat aaaatggcgg
    13321 ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta tgataaattc attcgcacga
    13381 cagatgatta ccataaagca gctgttcaaa aaatatttga tcgtttatta gaacagggag
    13441 atatttactt aggtgaatat gaaggctggt actctgtttc tgacgaagaa ttctttacag
    13501 aaacacaact tgcagaagtg tacaaagatg acgaaggtaa cgtgatcggt ggtaaagcgc
    13561 caagtggaca cgaagtagaa ttggtaaaag aagaatcata cttcttccgc atgagcaaat
    13621 acgcagaccg tctattggaa tactatgaag aacatcccga attcatccag ccagaatctc
    13681 gtaaaaacga aatgatcaac aactttatca aaccaggatt agaggatctt gcagtttcac
    13741 gaacaacttt tacttgggga gtaccagtca agagtgatcc gaaacatgtg gtgtatgtat
    13801 ggatcgatgc attatccaat tatattactg cactaggata tggctccgaa gatgaaagtc
    13861 tattcgaaaa atactggcct gcagatgttc agatggtcgg taaagaaatc gttcgctttc
    13921 atacgatcta ttggccaatc atgctgatgg cccttgattt gcctctacct aaaaaagtat
    13981 ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc taaatcaaaa ggaaatgtcg
    14041 tttatcctga aatgcttgtg gaacgttatg gtttagatgc actccgttat tatttgcttc
    14101 gtgctgttcc gtttgggtca gatggtgtct ttacgccaga agattttgtt tcccgtgtca
    14161 attatgatct ggcaaatgat ctagggaact tattgaatcg aacgattgct atgatcaaca
    14221 aatattgtga tggactcgtt cctgactatg cctcacttgt cactccattt gatagtgaac
    14281 tgtctacgac ggctgctaac gttgtcggac gttaccatga tgcaatggaa aaaatggaat
    14341 tcaatacagc attggctgaa atctgggtgc tgatttctcg tgcaaacaaa tacattgatg
    14401 aaacagaacc atgggtatta gcaaaagacg aagaaaagaa agctgaattg aatagtgtca
    14461 tgatccattt agctgaatct ttgcgtatcg cagcgatatt acttcagcca atcatgacag
    14521 aaacgccaga gaagatcttc aatcaactag gattggactc tgaaacaatg aatcttgaag
    14581 gattacactt tggcgaattt ccttcaggaa caaaagtagt tgcaaaaggt acaccaatct
    14641 tcccacgttt agatatggaa gaagaagttg cttttattca agaaaaaatg tctgaaggta
    14701 ctcaaacaaa cgaagataca gtcaaatggg atccagaaga aacagaactt gtttcaacga
    14761 aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga actaaaagta gcagaagtca
    14821 tcaactgtca aaaagtagaa ggggcagata agttgctgca attccgacta gatgctggag
    14881 acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt ttatcctgat ccaagcgaat
    14941 tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc acgaaagatg cgtggacaaa
    15001 tcagtcaagg aatgatcctt tctgcagaag caccagatgg ctctttacaa gtaatcgaag
    15061 caccaaaatc catgccaaat ggctcagaaa tcgcatagaa attcaaaggg ggtatgacac
    15121 tagttgtgtc ataccccctt tttccgaaat ttgagaagca attttcgtaa attccttctt
    15181 attacttgaa gccaatcata taatacagaa acagagaact atccaaaata aatcctagaa
    15241 aaagaagtac gagcttcgct gatttttgtt aatttagaat aatactaata ggaatcgtaa
    15301 aaacagaaaa aaagcataat gataaagggt cgggcagatt ttaattgatt catctggtac
    15361 tcttttttat tgtccttaca ttcataaagt ttgaatgcaa tgactttatt acatctatat
    15421 ttatatctaa cagtagcgtt gatgtgttaa ttataagttc tatcacttta tgttcaatta
    15481 gtttgtgctc ttatatgtgt gattgaaaat atagataaaa aaacaatcat cggctttgac
    15541 ttcgatgatt gttttcttta ttttgtaaaa atcctatcac ttgattttat ggtaaccttt
    15601 tatttttgtc ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg ggatcgaaaa
    15661 gacataccag aaaatcgtac gtccgatacc ataatgttga tagagctttg tcaacatcac
    15721 gtgtaacaag atcaacccta aaagcaagga gccagctata aaccaatgat tatcccaatg
    15781 catgacttca ctaagtgcca tcactccaaa aggatagaaa taaaaataga tcccgactaa
    15841 ggtcatccgc cagttctttg taaatgttcc gatattcaat aaaaagaaaa tcgctgaaag
    15901 caaaataccg ataggcggaa aaagcccaac caaaaaggaa tacaacatcc cccacaagat
    15961 catcacagga cctttgatca atgcaacgat tgcgatgaac aaagtcagca atattagttt
    16021 ttgtgataac gttgcaacag tcattaatcc aaataaaatt aatgtgaacc aatgccaatg
    16081 ggactgctgt tgttctttac gggaaaaatc gacccggttg gataaataat ctgctaattt
    16141 cggataagaa cgcttaaaat ccataaactg ttcctccttc gtggaaaagg taggtttagt
    16201 atacgaaaaa taaaaaagaa atacatccgt cttaagaggg agtttttcac tgaaagtgga
    16261 gaaaaaagaa tggtaaatta aaatattctc agttatactg aaaattatac caaatttcaa
    16321 agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct gtttggcgca
    16381 ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac tgctgctgtt
    16441 actgctagta ctcagtctac aagtttgaca ttgctagctt ggcttttggg tggtttctta
    16501 acgatctgtg ctggattgac agtggctgaa ttagctacag caatcccgga aactggtgga
    16561 gctgtaaaat atattgaagc tgcgtatgga aaattgccca gctttttatt aggatgggcg
    16621 caaagcttga tttactttcc tgctaatatt gctgcattat cgattatctt tgcgacacaa
    16681 ttgacgaact tattacaatt atctaccgat tatcttttgt tgattgcagt gatcaccgct
    16741 gtttctgtga cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt acaatcagtg
    16801 acgttgattg ttaaattaat accaattgct gtgattgtca tatggggatt attgacccca
    16861 ggacaaggaa ctgtccagtt atttccaatc gaagctggaa aagatgttac ttttgtcgaa
    16921 ggattgagca gtgcgttgtt agcaacgttg tttgcttatg acggctggtt aggtgtcggt
    16981 gcaatggcag gggaaatgaa acggccggaa aaagatttgc ctaaagcaat tattcttggt
    17041 ttgagttttg taacagtcgt ttatctattg atcaattttg tttttttgaa aactttgccg
    17101 attgatcatc ttgccggtaa tttgaatgca gcttctgaag catcagacgt catttttgga
    17161 ggaatcggtg gaaagctggt cactattggg atcttgatct cggtatacgg tgcattgaac
    17221 ggttatacac taactgggat ccgcgtgcct tatgcgatgg ctttagaaga cgagctacct
    17281 ttcagtaaac agctgacaaa gctttcaaag aagtttacgg ttccttatgt tcctgcggtt
    17341 ttccagttag cagtagcttg tattatgatg agccttggtt ctttcgactt tttaacagac
    17401 atgttgattt ttgtgatgtg gctattcagt ttactgattt ttatcggtgt ctttatttta
    17461 agaaaaaaag cgccggaatt gccacgtcca tataaagtac cactttatcc aatcgttccg
    17521 attattgcga ttctcggtgc tattttcatt ttaggtatga cgatgatgac gcaaacgaaa
    17581 ttagcattga tcggtatagg tgttaccttg ataggtatcc cagtctatta tcaaaaaaag
    17641 aaaaaacgct cagaagagta aaatatttca acaacagaca gaaaaagtac gaactaggtc
    17701 ctctagatct tcgtgctttt tctatttcaa taaaacaagt tcttctattt tgatttttgc
    17761 tacttctgca agtagtgctt atgtgctaaa gtaagaatga aaaggagaga taaacatgat
    17821 atttgattct catacccatt taaatgctga acagttcaat gaagatattc cagaaacgat
    17881 tcagcgagca caagaaatgg gggtaacgaa aatggcggtc gtgggcttcg atacgccaac
    17941 gattgaaaag tctttgcaat taagccatga ctatccaaat atatacagta ttatcggctg
    18001 gcatccgaca gaggctggca gttatacgaa agaaatcgaa aataaattac aagaacaatt
    18061 gactttacca aaggttgtag cattagggga aattggcttg gattatcatt ggatggaaga
    18121 cccgaaagaa gtccaagata aagtatttcg cagacagatt gctatcgcaa aagaaatgaa
    18181 cctaccaatc agtattcata ctagagaagc acttgaagac acgtatcaaa tcctaaaaga
    18241 agaagatgtt cgagatatcg gtggaatcat gcatagtttc agtggtgatt atgaatgggc
    18301 aaaacgattt cttgacttag ggatgcatat ttcatttagc ggtgtcgtca ctttcaaaaa
    18361 agcattagac gtccaagagg ctgcagcaaa tgtaccaatc gaacgattac ttgtggaaac
    18421 agatgcccct tatctggcac cagttccttt tagaggaaag cgaaatgaac caggctatac
    18481 tcgttataca gtagaaaaga ttgctgaact tcggaatctt ccgacagaag aagtggctgc
    18541 ccaaacatgg acaaatgcgc accgattatt tggtctaagc gaacatggat aagatgaaga
    18601 tccaagagat tatagtcgtt gaaggaaaag acgatacaag aaggctgcgg gaagtagttg
    18661 atgcagatac gatagaaaca atagggtctg caatcaatga agagatcctg atgcaaattg
    18721 aacatgccca agagactcgt ggagtgatcg tttttaccga tcctgatttt tcaggagaaa
    18781 aaataagaaa aatcattatg gaagctgttc ctcaggcaaa acatgctttt ttgccgcgca
    18841 cacaagctaa accaaagaaa aagggaagcc ttggtgtcga gcatgcaagt gatgctgcta
    18901 ttttagaagc attgaaaaag gtcgtgacgc cagacatttc ccaaacggct gtttctgaaa
    18961 tcactagaca agacctttta gactatggtt taatagctgg ggcacaagca aaagaaaaac
    19021 gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa cggcaaacaa ttggaaaaac
    19081 gtttgaagat gtttcgtatt acgccgaaag aactcgccca agcaatgaaa atagtggagg
    19141 aacaacatga gtgaatatcg tgaaattgcg acaccctcta gaacaaaaga gattttattg
    19201 aaacatggtt tttcatttaa aaaaagttta ggacaaaatt ttctgactga accaaatatt
    19261 ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata cgaacgtgat cgaagttgga
    19321 ccaggaatcg gtgcattgac ggaacaatta gccaagcatg caaaacaagt cgtagcattt
    19381 gagatcgatg atcgtttgat ccctgtattg gctgatacga tgcaacccta tgacaatgtg
    19441 actattgtac atcaagatat tttaaagacc gatttgtcaa cagctgttag agaaacattt
    19501 catgaggagc ttccgttaaa agtcgtagcg aatttacctt actatattac gacaccgatc
    19561 atgatgcatt ttcttgaatc agatttggtt gtggatgaac tagtcgtcat gatgcaaaaa
    19621 gaagtagctg atcgtatctc tgctgaacca ggaacgaaag cttatggttc attatcgatt
    19681 gctgttcaat attatatgga agccagttta gcattcatcg taccaaaaac agtatttgtc
    19741 cctcagccaa atgtagattc agcgattttg aaactgacaa gaagagatat accggcagtc
    19801 gaagtgacag acgaaaaagc atttttccga ttgacaaaag cggcatttca gcaaagaaga
    19861 aaaacgcttt ggaataactt gcagcattcg tatggcaaag atgaccagac aaaagcatgg
    19921 ttagcaaaaa gcttggaaac agctggaatc gatccaaaac gtcgtgggga gactttgtca
    19981 ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa atatgcagta aaaaaagagg
    20041 ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa
    20101 atagttcctc atgtttttcg tttgtttggg cttattaccg cagatgcaag cttttgaaca
    20161 ccgtttaaga aagaggttgt gggcctgccg tttaactcta agtcacaagg aacaatttca
    20221 aaaaacagct tctcatgttt ttgagttagt tggtcttatt acttgatgca agcttttgac
    20281 cactattatg ccgaacaagg agatgtgaca caaattttgt cacatctcct atttttgttt
    20341 cttgatcaaa aaattcttat acttgatact cacactcatt ttgtatggat ttagaaaaga
    20401 atatgtgtct tgacagttat tagtaagtga gttatactaa gtcgacattt atgtgtcaag
    20461 acatattagg agagagaaaa aatgagaaaa aattcagttc gaaaactaac gatttctgct
    20521 ttattgattg ggatgggggt cattatccca atggtcatgc ctaaaatcat gattccacca
    20581 gcttccttta ctttggcgtc tcatgtgcca ttatttattg cgatgttttt tacaccagga
    20641 gttgcagtgg ctgttgcgct aggtacgaca tttggtttct ttttaacaac gccggttatc
    20701 atcgcattgc gagcattgtc acatcttgtg tttgcgttga ttggggcttg gtacttgcaa
    20761 aagcatccga gtatcgtttt aaaaaatgga cagtttactt ttgctaattg gcgtttccaa
    20821 gggttcaatt ttgtgatcgg tgtgattcat tctttagcag aaatgctagt agtcagcatc
    20881 ttttacttta ttggtaatat gcctgaaaca tattattctc aaggatattt ttatacagta
    20941 ttcatcttga tggggattgg tgggcttatc cacagtttag ttgattataa tattgcttat
    21001 tttatagcag gaacactcag caaatatttt gatatccccg tatttacggc agcaaaaaaa
    21061 gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa cgacatatta gacatgatct
    21121 ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc ctctaagatg agagtttttt
    21181 gtcctaaaaa gagctgcacc aacatcttct ggcacagctc taacaggggg caatttatgg
    21241 aagtttgatg acttgttctg tttctttatc aaagaagtgg cctttgctga tgttgaaggc
    21301 aaggtcgact atttcgcctg gtttatggaa atctcttgca tctacttttg agatgaactc
    21361 agtagaacca gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg aaacgacaac
    21421 ttctgatttc actacggctt caggtgaagc ttcaagggct acttgttcac tgtggatgtc
    21481 ttctggacga ataccgaaga taacttcttt tccttcataa cctttttcaa ctaatacttt
    21541 gttttttcct tcaggaatac gaagcttaag cccatgtcca tctgtgataa caccattttt
    21601 caatgtcaca ttaaagaagt tcatggcagg agatccaata aagccggcaa cgaacatatt
    21661 gacgggtgta tcataaactt ctttcggtgt accgatttgt tggataaatc cgtctttcat
    21721 gataacgatt cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa catagatggt
    21781 cgttgtttca agacgctggt gaagtttggc aatctcagca cgcatggcta cacgtaattt
    21841 agcatctaaa tttgataatg gctcatccat taagaagact tttgcatccc gaacgattgc
    21901 ccgtcccaat gccacacgtt ggcgttgtcc accagataaa gcagctggtt ttcgtttcaa
    21961 gtattctgtc agacctaaga tctctgctgc attgtccaca cgttttttga tgtctgcttt
    22021 atcatattta cgtaatttca agccaaaagc catattatca aaaacagtca tatgaggata
    22081 caatgcgtag ttttggaaaa ccatggcaat atcacggtct ttaggtgcca cgtcattcat
    22141 gacttttccg ccgatattta gttctccttc agagatatct tccagtcccg cgatcattcg
    22201 caaagtagtt gactttccac aaccggaagg accaacaaaa acgataaatt cacggtcttt
    22261 gattgataga ttaaaatcag ttacagaata attttctgca ttgtcatatt ttttatatac
    22321 gtttttcaga gccatttcta ccataatacg ttccacactt tcttttttag ttttattaac
    22381 tatttaaagt ataaatgaaa gcgttaacta acttcaatgt catgttgaac aagaattttt
    22441 tctttttttt gtcaatgtgc tagttttaag ctttaataaa tatccataag aaataaaaac
    22501 tgatacactg gagtaaataa gtatgttaac aatatcaaaa ggagtgtctc ttatgaaaat
    22561 cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg aaattagcaa cagcgtataa
    22621 aacaatattg gaaaaacatg aactttttgc taccgggacg actgggcaga ggatcacgga
    22681 agcaaccggc ttgccgatcc atcggtttaa atccggaccg ttaggcgggg atcagcaaat
    22741 tggagccatg atttcggagg atcagttaga tttggtgatt tttcttcgag acccgctggc
    22801 tgcacagccc catgaaccag atgtaacagc acttatccgt ttatctgatg tctatgagat
    22861 tccgttagct acgaatatcg gaacagctga ggtgctacta agaggactgg aagccggctt
    22921 cctagatttt cgaagaatcg tccatgaaat agacaagcat ccactttcat tttgatggat
    22981 aagcgtgtgg aaaaataacg tttaatgttc gcctatatgt agctataaaa gctaaacaaa
    23041 ctgataatat gcgatataat acgaacgtat tggataatat aaacaataat attagttttt
    23101 cgtatttgca gtaaatgtga aaatatgcta tagtttctgt gggaaaagat cccaaaaata
    23161 atacagagta gaaaataaag cgttaagtgc taagaggatg ggatgttgcc tcttggacga
    23221 aggacgtaat gttcgcggtt ttattttcgc attcgctgca tgatacgatg tagcagctct
    23281 taaaggagat gctaaaaatg aagaaacacc ggttagacgc acgtatgatt gctattatgg
    23341 gcttattgat tgcattgatg gtcactttgt cccgactggt agcaatcgag accccattta
    23401 ttaaaatcag tgtcacattt attccacaag tgatcatggg cattcttttt ggtccttttt
    23461 ggtcagggat aggcgcagtt ttagctgatt tagtaggaat ggccctcttc tcgaaatctg
    23521 cattctttat tggatttaca ttgaatgcat ttattgaagg agcaatctac ggattctttt
    23581 tctatcgaaa agagatcact tggaaaaatg ccattctggc tacgcttagt gtcacgctga
    23641 tcatcaactt atttttgaca ccattatggc ttgcgttgat gtaccatgtg ccattgttca
    23701 gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat atggttaccg attcaatcaa
    23761 ttgccattta ttacgtagga cgtagtattc cttacaaaaa aattttaaga agtttagcga
    23821 tccacgctaa ataaaaaaca agcagtcttc ccatcccaga agactgcttg tttttattgt
    23881 gctgtgaatt gtgcttgata gagccgtcgg taataaccat ctttttgaag cagctcatca
    23941 tgggtgccca tttcgacaat agctccctga tccatcacta atatctggtc cgcatttttg
    24001 attgtggaca ggcgatgggc aatcacaaaa ctggtttttc cttccatcat tgctaagaag
    24061 gcttgttgga tttttttctc tgtcaatgta tcgactgagc tggtagcttc atctaagatc
    24121 aacatagggg gattactgat catcgttcga gcaatcgtca acagttggcg ttgtccatct
    24181 gatatcttta acccttgacc gccaatctta gtatctaatt tttccggcaa acgttctaca
    24241 aattcataca tataagaggc ttttagggca tcattgattt ctgcgtcact tgcttccgga
    24301 ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata gccaagtatc ttgtaacacc
    24361 attccaaaac ttttacgtaa tgtatcacgt tggatagagc ggatgtcatg gtcatcgact
    24421 gtaattgttc cttttgttgt gtcataaaaa cgcatcagca aattaacaag tgtggatttt
    24481 ccggcccctg tttttcctac tatagcaatg gtttgacctg gataagcagt aaaattgaaa
    24541 tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa catgatcaaa agagactttt
    24601 cctttgatat ttttgggttc caaggcatag gcatctggca aatcaggctt ttcttctggc
    24661 tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag cggtttgcag ttgtgtcgtg
    24721 attccagaaa gttcgataaa tggtttagaa aactgacttg agtagatcgt aaagctggaa
    24781 atcacaccaa cagtgattga cgaaccactc ttcaatgcca ataggccgcc aactaaacca
    24841 atcgataaat aagcaaggtg atctacaaaa cgagaagacg ggtttgtcaa ggaagaagaa
    24901 aattgtgccc gttgtccttt ttcatataga tcagcattta gcagttcaaa ttgctgttga
    24961 ctagcttctt cttgctgaaa agctttcacg attttctggt taccgattcg ttcggtcaca
    25021 aagcccgaga tatccccaat aatcctttgc tgtctatcga aatttgtttg tgaagcacga
    25081 gcaacgatcc aactcacaag gaaaataatt ggtgtagaac aaagaacgac caatgtcaga
    25141 agtggactta gacgcatcat aaaaataaac gaaagaatga tgattgccac tccagaaaat
    25201 aattgattga aggccgcaga aattgcaatg gaaatattgt ccatatcatt tgtaaatcga
    25261 ctgacgatat ttccgtggga gttttgatca taataacgaa gtggcaatcg attcagatga
    25321 gcaaatgcat cttttcttaa ttttgcaacg gataagtaag ctaaccgatt gcttaacact
    25381 tggatgagcc attgactgac tacgttgatt aaaacgatac caccgaatag aataagtaaa
    25441 tggaacagtc gattgaagcg gacctgtcct tctccaatca tttgatcgat actttgacca
    25501 atttgtaacg tcatcacaac tgttgcaatc ccattgatga ttcctagtat cacagctagc
    25561 caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc ggcggagcgt ctgcaaggat
    25621 aattttttta ctttcataac tattcttcct cctgtgatgc gacgatttcc tgatattctg
    25681 ctgattgagc aagtaattct tcatgggtac cctgagcagc aatcttacct tgatccatca
    25741 ctaagatatg atgtgcttct tgaatagagc ggacacgttg ggagataata atcactgtac
    25801 tgccaagata agtctgtagc gccttacgca aatccaagtc tgtttgataa tccaacgcac
    25861 ttaaagagtc atctaaaatc agtaatgctg gcttggcaat caaagctctt gcaatcgtca
    25921 gtcgctgttt ttgtccgcca gagaaatttt tcccattttc catgactttt gtatctaaac
    25981 catttggaag ctgacggaca aattcttctg cctgagcgat tttcagagct tcccaacatt
    26041 ctttttctgt ggcgtctttc ttaccccatt gaaggttttc acgaatagtc ccggtaaata
    26101 gtacagaagt ttggggaaca agagcaattt gctgacggag atgatccagc tgccaagaac
    26161 gaacatccat tccgtccatt ataactgtac cggaactgac atcatagaaa cgcggaatca
    26221 attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat gattccaagg actgtatttc
    26281 gcttcagctg aaatgtaata ttttggagag aaagaccaga atcaggagta taacggaagt
    26341 caacatggtc aaaagcgata attgcttcag aagaaaaatc gggagtagta ctgacctcag
    26401 cttcaagaat cgaattttct gtgtctagca cctctttcac ccgatttcct gaagcttcgg
    26461 cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag tgccaaaagc atttgattca
    26521 tataattgat caaagcaaga acttgtcctt gttgcaagct accaatattt actttgatcc
    26581 cgccaactgt cagcaaacag atgatcccga cattcatgat caaggttgta gcaggtgata
    26641 atagggcaga gatattggaa acacgtaaat aattgttcgc tagatcattt gtgcttttat
    26701 cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg gatgacacga acaccgctaa
    26761 gattctgact aacgtgttca ttgagctgat ctagtttttc ttgcactttt tggtagagag
    26821 gaacggtttt tttgatgata aaaaataaaa tgattgagaa aataggcaat aatgctaaga
    26881 aaaacagacc tacttcccag tcgatcacaa aagccatgac cactgacccg atacttaaaa
    26941 acggtgctct gatcaataaa cgaatgacca ttgctaaagc tagctgaagc tgattgatgt
    27001 cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg attcaactcg ttgtgagaga
    27061 aagtattgat ttttttgatt agctgattac gtaactctgt accaaatcct tgtgaagcta
    27121 ctgaagcata gtattggcaa atgatagcac aaatcagacc aatcacagac atagcgacca
    27181 tccataagcc catttcaatg atttttcctc ggtcattcat ttttagtcca ttatcgatca
    27241 aggaagccat cattaaaggc aggacaagtt cgaagacagc ttcaagaaat ttgaagaacg
    27301 ggcccaaaat gatttgctta cgatagtctt ttgcataacg taataactga atcatatgat
    27361 tcctcctatt atttatttgt ccacaattga aaaaacaagt actaccacat tgtagcttag
    27421 gaaatttaga atgaaaagag aacaagcctt ttcataaatg aaagttttat tatgaagaag
    27481 ctttagaaat tgaatagact atttttttta aaaaaaagaa aatgagaaaa catgccagtc
    27541 tcttcattca taggaacgtc tattttactt aatttttcta taaatgaatt ctgcataaaa
    27601 aatatatcaa gtaaatgttt atagatgaaa ctctatttac ttctttttta atttgttctt
    27661 gcaatctttt ttgctacgta atttttgtaa aaaagtgggt tgagagaaat ctaattgatt
    27721 ttcaaagaag gagtatgaaa atgagaaaaa tatttattcg tttattgtgg gtactatctt
    27781 agaggaataa ataatagtgg agaaaactat ggtttggcgc agattctatc aggtttgatg
    27841 gaacgaagac aacctcccga catcaagtca aaagctactt gagattttga tctgaaaata
    27901 aaaaaagaaa acaaggtagg aacatcaatg tttctacctt gttttcgtaa tcaatgggaa
    27961 aaaactaaga gtggtgtacg taatcaataa aaaggatggt gacaacagta cctctttact
    28021 ctctgaaagc atcataactc gtttttcctc attatgccaa taaattgttg ttataccaca
    28081 tataaggatt tgtaatagct aaaaacagac aggaaagttg aaatctgtga atcttatttt
    28141 gtatggaagg atttatagaa cgcaaaaatc aagataaaga tgaataaaaa tcctgtttcg
    28201 gctatatatt gcgtatctac gccaataaaa cgagtttgtt ctgtcatagc catataaagc
    28261 aaaggcaaaa atacaactac agcgagataa gatccgatga tttctcgaaa agttttccag
    28321 gaagtatgtt tcctgttttt atatttatcc gatttcattg aatagtcata gcctccttat
    28381 tctttcatat gttgaaattc cagtataaaa actggaagga actgtacgtt ttttagaata
    28441 aaaattataa gggataagta ggctgctgtc tataataaga tatatttttc aattctttat
    28501 taaagatttt attgacaaaa tgaatccgtt ttctatgatg gaggaggggt gaataagttg
    28561 ttgaagaagg aaattttatg ttacttagag aaccagacag cctttttcga tttagaacat
    28621 atgaacgagg tctttacagc acaaaggtta gctgatcatt ttggggtaaa aagaaacaca
    28681 gtcagtcatt atttgaacca gttaaatgat gagcaactac tagtcaaaat caattctcga
    28741 ccagtgattt atttccacaa agaagctttt gaaaaacaat tttttaagtt acgcacgaat
    28801 ttttattttt ctatcgatga tctcaaagag gaacagcctt ttttcgcgca accaaaagac
    28861 ttattctctt ttatgatcgg atacgacgcc agtttgaaag aaagtatcga acaaatcaaa
    28921 acagctcttt actatccaga tggcggatta ccacttttga ttacaggcga aagtggcaca
    28981 ggtaaaagtt atttagttaa tttggtctat cagtattgtc tgcttcatga tctgctagaa
    29041 gattctgcac cattcatcac ttttaattgt gcacaatatg cagataatcc tgaacttttg
    29101 actagcaatt tgtttggaca tgttaaggga gcgtacactg gtgcagaaga aaataaaaaa
    29161 ggggcttttg aagcagcaga tggtgggatc ttgtttttag acgaggtaca tcgcttaaca
    29221 ccagaaggtc aggaaaaact gttcacctat cttgatcaag gtgtgattta tcgtatggga
    29281 gaacctaatt tgtcacgccg gatcaaaaca cgtctctttt ttgctacgac tgaagagatt
    29341 actagtcatt tcttaacaac tttcattcga agaataccta tccgaatcga actacctgca
    29401 cttaatcagc gtagtcgtaa tgaacggcta gaactgattt actctttttt tctcgaagaa
    29461 caaagaaaaa tggggagccc tttgtcagtc agtggaccag ctttgtcact tttaacaagt
    29521 cagaaattat ccggaaacat aggagagctg aaaaatatcg tcaaagtttc agcagcaaaa
    29581 gcatatgctg aacagcgtga acagcctgaa atccatgtca cgatccatca tttgcaaaaa
    29641 gaactacttg cgcagcctat tactaaaaat acagcacagc aaggaatcta tattacgcaa
    29701 gaccagacct tagagcaatt aatggagaag caacagcccg aacagcagcg aatcgttaaa
    29761 agttttgagc aaatcctaat tgatttcaag aaaaataact gtctgctata ttcttgcgaa
    29821 ggaaaattaa agcaagaagt gattcaatta tttgattttt tgctgtttga gaccagtaga
    29881 caagaaaaac acgagctgct ggtttattgg acacaatcta tccgagagac ctttcgacaa
    29941 atggaaactg cctatcaaat caaattcgat ggaaacagtg tctatgcctt gagttactat
    30001 ctctatcagc gaagcactat caaatggtta ccggaagaac atgatattat ccaactaatc
    30061 aaagaattag aacaacaagt ttctcagtct tatccatcta gttatcacta tgtccaacga
    30121 attctggaat taagtaagat caaattagat attgaagaca caagtatgga tcggatttta
    30181 ctgacgatct atctaaaaaa agcgaaatgg tcaaaagaaa aacgactgcc aaaagcgatg
    30241 attgctgctc acggttatgc aactgctagc agtatggcaa atgtagtaaa tcgtttatta
    30301 caagaggatc tatttgaatc atttgatatg ccactagatg tcacaccaca gcagattgct
    30361 tcagaaatcc ttgattattg cgaaaacagc gatgtttcaa atggattgat catattagta
    30421 gacatgggat ctctgaaaga aatccatcag ttttttaaaa aacagttatc cgtaccgctt
    30481 ttgattttga ataatgtgac gacaccttta gcaatcacag taggggaatg cctgcaaaaa
    30541 aatgtatcat tagaagagat agcagaagag actgttcgac agatccagcc agaatggcga
    30601 ctgctctatc cagaggaaaa caaacccaaa gcactcatta ctacttgctt cactggaatc
    30661 ggtacggcgg tccacttaag tgaactgcta gaaaaaagct tacctacggc ttgtcagttg
    30721 aaaatcatcc catatgaata tcagcaatta aaagataaga aaaacagtga cccattattt
    30781 tccatatacg aagtcgtagg aatgattggc accacagatc ctgatatcac caatatcgac
    30841 tatctttcat tagaagacct catctcgggg gaaaagatgt ctgtactagc tgaatggttg
    30901 gacactacta tgaatgcttc agagaaggaa atattcaatc agcgcatcat tcgtaatttt
    30961 tcattggaaa aagtactgga ttccgtgact attctagata cagagaaggt gatgggagaa
    31021 atcgatcatt ttatgagaga attagaggtg cagttgaatc gaagcatcag caatcccaaa
    31081 aaattagcgt tgtatgtcca tgtgagctgt ctcattgaac gattgatccg acaaatgcca
    31141 atcgaaacct atcaaggatt agagaaatgg aagcagtgtc aaaaagaagg gttatctgct
    31201 attaaatctg cgtttagtgt cattgaagag cattatagtg tcatgatccc caattctgag
    31261 attgcctata tctatgatat tttgagtgaa gatacagagt ttctctcgat agaagaagaa
    31321 ttttgaacat aagcaagttg ttgccactaa ttttggcatg caacttgctt ttttagtatg
    31381 tgtaagtgaa aagaggagat gaaaaagtgg atagagaaat tatcttagct tcccatggaa
    31441 aatttgcttc agggatatta gattcattag agctgatcta tgggaaaaat cattctatta
    31501 ctgtattcga ttgctatact gatgagaatt ttgatttagc ggaaacagta aggcagcttt
    31561 ttatgaggta caaagataaa gaaatcattg ttctgacgga tctttttggc ggaagtgtca
    31621 acaacgaatt cttacaatat atcaatcggg atcatgtcta tctcgttgcc ggattgaatt
    31681 tgccactatt gattgagcta gtatctcggt tggacgctga agtagagaca actgttttga
    31741 tccagcaggt gctaactgat tcaaaaaaaa tgatccagtt ttgtaacgag cggctcaatc
    31801 agaaaataga agaagacgaa ttttaggagg aagaaaaatg attgtattga caagagtcga
    31861 ccatcggtta ttacacggac aagtagcttt ttcatggaca caaacaatag gcgcagactg
    31921 tattttgatt gctaatgatg atgtacctac gaatgagata cgaaagacaa cgatcaaatt
    31981 agccaagcca caaggagtca aactagtgat caaatcaatt gatgactcga ttgccgcgtt
    32041 gaaaagcggg gtgacagata aatataagtt attcatcgta gtggaatcga tcgaggatgc
    32101 atataagctg gcagaagcgt atccagaaat caaaaccatc aacttaggag ggatcaaggc
    32161 aaaacctggt actcgcagca tcggaaaagc ggtaaatatt ttaccagaag aggaggaacg
    32221 cttgaaacga ttaattgaca aacaaataga agtagaaatc cgtcaagtac cagcagataa
    32281 aaaagtggat gtaagagaag ttttgtaaga aaggaagaac ctcaatgatt gtacaagcag
    32341 tcttattagg aattgttgca tttgtcgcac aatcagaata tgcactaggg acatcgttgc
    32401 tttctcgtcc gattgtgacg gggctgttta caggaatcgt gttaggtgat gtaaaaactg
    32461 ggatcattat gggcgcaact ttagagttag catttatcgg atcgttctca gtaggtgctt
    32521 cgattccacc tgatgttgtg accggtggga ttttagggac ggcttttgct attacagctg
    32581 gagccggtac agaaaccgcg cttttactag gtttgccgat tgctacgttg acattgatat
    32641 tgaaaaatat ttacttaggg cttttgattc caattatgaa ccataaagca gacaattatg
    32701 cagaagaagg aaattataaa ggagtcgagc atatgcactt gcttgccggt atcggtcttt
    32761 ccttgatgct tggtgttgtg gtaatggttt cttatatggt gggaagcaat acgatcggaa
    32821 atctattaaa tatgattcct gacttcgttc aaaaggggtt gtcagtagct acagggttga
    32881 ttccagctct tggttttgcg atgcttgctc gtttgctgat caacaaacaa gttgcaccat
    32941 attttttctt aggttttgct atagcttctt acttagagat tcctgtgacc ggagtagcta
    33001 tttttggtgc gattttagca gttgtggtag tcaatattat gaatgtccgt caactaccac
    33061 ttcaaacaac tgcaggggag gtagaagaag atgaagaatt ctgagatcaa aaacagcaaa
    33121 ataacgaaaa aagagctaaa tcacgtattt tggcgttctt ttcaaatgga gttttcttgg
    33181 aattatgaac gtcagatgaa tatggcttat gtttatgcaa tgattccgat tttgaaaaag
    33241 ttatatcagt cgaaagaaga aatggcagca gctttgaaac ggcatctaga gttttttaac
    33301 acgaccccac atatcgttac actgatttta ggaatcacgg ctgcaatgga ggaagaaaat
    33361 aaggatgatc ctgaatttga tgtgactgcg atcgatagta tcaaaacatc tttgatggga
    33421 cctcttgcag ggttaggaga ttctttcttt tggggaactt tgcgattgat tgccacaggt
    33481 gtgggtacct ctttagccat gcaaggaaat attttaggcc ctatcttgtt cattttgatt
    33541 ttcaatattc cgcacattct gtttcgctat ctagcaacag gttggggata tcgcttagga
    33601 acgggttttt tgaaaaagat ccaggcaaac ggtatgatgg aaagtttgac attaggcgcg
    33661 tctattattg gactgatggt cgttggagca atgactgcca cgatgatcac catcaatatt
    33721 cctttaaaaa tcggttcagg agaaaatgct gtgactgttc aaagtatttt tgatgatatc
    33781 gttccaaata tcttatgtct tggagcattt ggcatagtgt tttatctatt gaagaaagaa
    33841 gtgaaaccat taacgatctt gatcggacta gctttctttg gaatttttgg ttcgtggata
    33901 gggatttttt aaaaaaggag cagagaaaat gtttacgatg caagattata tctatgaaga
    33961 aaaggaagta ctaagtacga tcttgaaaaa gaatgatttt tcgacaagag aacatatgaa
    34021 aaaaacagtg aatttactga tcttagcaac gggttcatct tataacgctt gtttagcagc
    34081 gaagccagca ttggaatctt atggtgatct tactgtcgat attcaagaac ctttttattt
    34141 tttcaattat ggaaaacttt ctccttcaat cgatactgtg attgctgtgt cacaaagcgg
    34201 gaaaagtgct tctactgttg aagcagtaaa aatgatacaa aaacaagggt tacctgttgt
    34261 tgctatcaca aatgacgtgc aaagtccact tgctttagaa gctgcacaaa ttattgattt
    34321 aggtgccggg gtagaatcag taggcttcgt tacaaaaggc tattctgcga ctgttttaca
    34381 gttactactc ttagggatag gaattggaat aagtaaaaat aaaatcagca aaaaaataga
    34441 acaagactac atgcaacagt tgcgcaaaat aatcaaccat ttaccagcga ttattcaaaa
    34501 aacggaagaa ttttttgatg gatatcaaag tttatttcgt ttagcacagc gtttcgtcac
    34561 cattggatat ggtccaaact ggggaacagc taaagaagca gagacgaaat tgactgaaac
    34621 aatccgcgtt ccgtcacaag gatttgagtt agaagcttat atgcacggcc catacctgga
    34681 ggcagatgct tcgcatttgc tattctttat tgaaggggaa agtgtaaata aggaacgttc
    34741 gcaaaagttg caaagatata tgagtcggta cgttggagag actctcacca ttaccacaaa
    34801 aaaagcaaga aacgagaaaa cacttggatt agcaatagaa tgtgatgaat atctgtctgt
    34861 attagcgctt gtcgttccgt ttcaactatt cgcttataaa accgcagtag ctaaaggtat
    34921 tgacttgaac aaaaagattt ttgaagactt tgatacagtc ttgaaaagta aattataaga
    34981 ggtggaaatc atgctgaaat tcaatgaaga agaacaaatc aaagcaataa aaggcgcact
    35041 agctttacgt ccgcaagtag aagaaatcat cgataaactt tatgcggaaa aatttgacgc
    35101 tgtctattat ctaggaatcg gtggaacgta tgcttcctcg atgcaagctg tcacttatat
    35161 gaatgggaaa agcaacttgc ctgtctttgt acagcatgct gcagaatatt acactactgg
    35221 aaacaaaaga ttaacaaaag attctatcgt tattttgtcc tctgttaccg gtacaactca
    35281 agaagtagtg aaagcagtag aacaaatcaa gaaagtagga gcgacactga ttggatttat
    35341 cgataaagcg aacagtaagt tgtctcagct atgcgatttt gtggtcactt atccagctcc
    35401 tggaacagaa caaatcaaat tttttatggc agcagatcga ttgatgtatt tgaatggaga
    35461 attcgaagct tattcagaat actatgaaca gctcgaacgt tatttaccaa cagggttagt
    35521 ggaagcagag aaaaaagcag atgcatttgg cttatcattt gcagaaaaac atcgacgtga
    35581 tagtatgcat tattttattg gagcagggaa tcaatgggga gctgtttatt catatgccat
    35641 gtgttactgg gaagaacaaa gctggcttcc atcgaaatct atccatgctg ccgagtttct
    35701 tcacgggacg ttagagatcg tagaagaaac aaccccagtc acattatttt taggcgaaga
    35761 tgaacagaga gtgttatctg aacgtgtggc taaacttttg ccaaaaattt gcagcaatta
    35821 tacattgata gacacaaaag actatccagt agaaggtatc agtgaaaaat atcgcggacg
    35881 tgttttgtct ttcttattga tgcatgtagt gacacaacgc atcgatgctc atgtggagca
    35941 gttgaactgc catccattag agatacgccg ctactatcgt cagtttgatt attaggatag
    36001 actggataaa aagaatataa aaaataaaga gagcagcgat aactgctccc tttatttttt
    36061 taaaatattg gcatcaatta cgttataaaa cacatttttt gcattatcta cagttcaact
    36121 tataatctgt ttaatgggac atgtgggtgc caaccatttt ttgttatgca atagtactat
    36181 ttagttgttg catggatccg tagtaaaagt ccaagtacaa cgagttgcgc cagtttacat
    36241 agcgttttgt atccagaatg ttgactaaaa caaaagttcc tatgtcaacc gtcaacagag
    36301 acaatctgtc cgttgcagga cctgacagat ttagaaatgt tcaatctgtc tcttatgtat
    36361 tttaaatcaa tactcactag gagaagtttc ctaacctcct aatagtaatg ctggtagtaa
    36421 cattgtcaaa actgcttcct cttaggaaca agcaaagcat aacgagtgta aaaataaaat
    36481 gtagactatt gtaacatatc attgattatt tcaagtaaaa acgataaaag aagtaaacaa
    36541 acacttaaaa acaatggaaa ttagtcccaa ctttaagaaa tgtatactat aatggaaatt
    36601 atcaattatt tatttggagg aatatccatg aaacaaaaaa tcgcagttct tggtcctgga
    36661 tcttggggca ctgctttagc acaaacattg gcagaaaacg ggcatgatgt ccgtatttgg
    36721 ggaaatgtac ctgaacagat tgatgaaatc aataagtatc atacaaatca gcatttttta
    36781 cctgatttga cgatcccaga atcgattatc ggatataaag aattggcaga agcagtggat
    36841 aacgcagatg ctattttatt tgttgttcca acaaaagcga ttcgttcagt cgcacaggaa
    36901 cttattggta aaatgaatac taagccaatc attatacatg caagtaaagg actagaacaa
    36961 gatacgcata aacgaatctc agaagttttg gctgaagaaa ttccagaaga aaaaagacag
    37021 gcaattgttg tattatctgg tcctagccat gctgaggaag tggcagtaca tgatatcaca
    37081 acaattacag ctgcaagtat tgatcaaaaa gcagcagcct atgtacaaaa actgttcatg
    37141 aatgattatt tcaggattta tacaaataat gatgtgattg gcgtagaaac tggtgcagca
    37201 ctgaaaaata tcattgcaat tggtgccggc gctatccatg gattaggatt cggagatgat
    37261 gcaaaagcag caatcatgac tcgaggattg gcagaaatca gccgtcttgg tgtcgcgatg
    37321 ggtgccaatc ctctgacatt tatcggtttg agtggtgtgg gggacttgat cgttacttgt
    37381 acaagtgttc attctcgcaa ttggcgtgcc ggtaacttgt taggacaggg acataagctg
    37441 gaagaagttc tagaaaacat gggaatggtc gttgagggtg tggcaacgac aaaagcagca
    37501 gtagagctag ctcaacagct aggtgttgag atgccaatta ctcaaacaat ctataatgtt
    37561 ttgtataatg gagaagatat caaaaaagca gcaaaagaaa tcatgttacg tgacggaaaa
    37621 atggagaatg aattttctct tcgttaaata gatgatcaat agttaatcgt
//
LOCUS       DAFEHS010000018        35714 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFEHS010000018
VERSION     DAFEHS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.018"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            310..474
                     /locus_tag="LP327_001003"
     CDS             310..474
                     /locus_tag="LP327_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725138.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            471..698
                     /locus_tag="LP327_001004"
     CDS             471..698
                     /locus_tag="LP327_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725139.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PKEVDKCKLVYCGHGYWKDEYIQQNIERYIK"
     gene            763..1338
                     /locus_tag="LP327_001005"
     CDS             763..1338
                     /locus_tag="LP327_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725140.1"
                     /translation="MKLKDYIREGYNIVTSPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNSPAVLRAKPLQNAYAIERFSCEFTGYKIVVPIMTEETKRKVMQQTKKR
                     IDELALDDRSNKNLLEIKLKDTDSVPEVYYKGQRMDEMPRGLIDITYHWHTEDDEYQV
                     PRGVNDITIEYYDSPNNKYLDRKIIGHKRDM"
     gene            1339..1656
                     /locus_tag="LP327_001006"
     CDS             1339..1656
                     /locus_tag="LP327_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL1725141.1"
                     /translation="MKEARPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(1814..1978)
                     /locus_tag="LP327_001007"
     CDS             complement(1814..1978)
                     /locus_tag="LP327_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HBL1725142.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2080..2601
                     /locus_tag="LP327_001008"
     CDS             2080..2601
                     /locus_tag="LP327_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBL1725143.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTDELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2588..4378
                     /locus_tag="LP327_001009"
     CDS             2588..4378
                     /locus_tag="LP327_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL1725144.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKTSLISGLSTYMVA
                     YDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFGKPKRSSINYAPAFSKME
                     PRASDSRKLDGLNTHFGIFDEIHEFTNYKLINVIKKSRGTRKQPLIVYITTAGYVLDG
                     PLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPRLWIKANPNICLMNFVGM
                     LDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKRNNKTIDIETLKGKKCVG
                     GFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDNNQERIKAWEKVGDLTII
                     PGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELENAGFETNEIRQGFLSLG
                     GPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWMPSKQSKSRKIDGFAASL
                     NSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            4394..5614
                     /locus_tag="LP327_001010"
     CDS             4394..5614
                     /locus_tag="LP327_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL1725145.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKISRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRAEKAKGYRYKFNIAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            5649..6326
                     /locus_tag="LP327_001011"
     CDS             5649..6326
                     /locus_tag="LP327_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBL1725146.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRSYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKADAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLIIKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            6323..7516
                     /locus_tag="LP327_001012"
     CDS             6323..7516
                     /locus_tag="LP327_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL1725147.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTASGSRVYEKWSD
                     IKPLTALDTEDGEIPANDNPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRTDGSYLLQKDVTSATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAEG"
     gene            7531..7857
                     /locus_tag="LP327_001013"
     CDS             7531..7857
                     /locus_tag="LP327_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL1725148.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAVQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            7861..8196
                     /locus_tag="LP327_001014"
     CDS             7861..8196
                     /locus_tag="LP327_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL1725149.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFTKDFT
                     TIIAKEVSK"
     gene            8193..8588
                     /locus_tag="LP327_001015"
     CDS             8193..8588
                     /locus_tag="LP327_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725150.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            8585..8956
                     /locus_tag="LP327_001016"
     CDS             8585..8956
                     /locus_tag="LP327_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725151.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            8972..9532
                     /locus_tag="LP327_001017"
     CDS             8972..9532
                     /locus_tag="LP327_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL1725152.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTHASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDGRGY
                     VFMTAVESTDMTLQKFKDLVNNKAGK"
     gene            9657..9968
                     /locus_tag="LP327_001018"
     CDS             9657..9968
                     /locus_tag="LP327_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725153.1"
                     /translation="MIELQLKLDGKKKIFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQMASKK
                     R"
     gene            9992..10183
                     /locus_tag="LP327_001019"
     CDS             9992..10183
                     /locus_tag="LP327_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725154.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVENGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10183..14568
                     /locus_tag="LP327_001020"
     CDS             10183..14568
                     /locus_tag="LP327_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL1725155.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKSAGNEAG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGTVAGLASQA
                     VSGVVNSFMGLTDEAVEASDSMDKFVQTMQFAGIDNSKIEESKTVMKDYADQTVYELG
                     DVMNTTAQLAANGVKDFDGLTQAIGNVNAVSGGNADTFKSVAMAMTQTVGAGKLTTEN
                     FNQIADAIPGASGKIQQALKEMGAYTDGDFRNAMANGEISAEELNKAFMQLGMTDVAK
                     KAAGSTATIEGAVGNLQASAVNMINEIINAFGKGNITSLISDIGKGIDNLTTKIKPAM
                     ESIKNVAQPVFDMLKKGADIATLAFGTLFGTLDGDQAKQGFDILTKIFPPEMVTFAQD
                     TIEKIKNAVKTLFDVFKGNADMEDVLAKLGFSPETISKIKDVFDNLKTYFDGTMTTIK
                     NGIEIGWQIAKGIFDTLSPYIMPIIEKIGGAFSKIGKSMTTFWNENGKQITEAIKNFF
                     TFIQPVVKIVMDLVMGFIDNIIGLVEGIMNVIQGAIKIFTGLFTGDFSKMWEGVKQLF
                     WGSIQAVWNWIQILFFKRILEGVKGLWTGFSGSIKGLWESTKTFFTEGISKTWDKLVN
                     WVMNLLGKVGNLKTTFGNFIQNMWNSVKNFFSNGVGDTWNKVVGWVKNIFNKAIELKN
                     NVSDAIGNLWKGIKDTFRRGIDTVFNWFSELPNKMKDAIIGGKDALVDAFKSIFNAAL
                     KAIGKPVNAIINGASWVLEKLGADKLKEWEVPQYAKGTPAGGHPINGPMMVNDGRGAE
                     TVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNLWN
                     SVKNVAGNVGNTLKNVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFQVTSGYREGDPYS
                     HGKHNAIDIALPGVVNGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGVHD
                     DWRTWPDGDHYDHVHLNGVRDPQGGLVSGGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPRAINNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIYD
                     PLSNILASIRYALSRYGSLRNAYRGHGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLAIAQDKLGVKPKSVNNSSDSSGTLETLVSLMIQQNNLLSKLLAKDTSVKL
                     DGKAIADNTNGYLGNQLKRSLYTTG"
     gene            14579..15409
                     /locus_tag="LP327_001021"
     CDS             14579..15409
                     /locus_tag="LP327_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBL1725156.1"
                     /translation="MNGYLIDFRFIKNQEVVSLKEKLGIECISFVRKAPQLNVEYQEF
                     SGSNGSREVEKSFKSFTIEVEFYAEFKNMYDYQLKETELYAFLFDDEGYYVFTDREPG
                     KKYFVRPNSVEVNEVGLRYATYKTTFTVFRGCSESMASTLSEFSLSNEWQFSQGLVAE
                     DCKYTHRTSNFIIYNAGDFAIDPREHALKITLEGESEGNVTIFNKTTGERFIYYPEFS
                     TLLGQTLTLDRVYPKLNGVNCGIDTNLGLITLAVGTNEIEIQNVTRVESKWDFNFLYK
                     "
     gene            15418..16437
                     /locus_tag="LP327_001022"
     CDS             15418..16437
                     /locus_tag="LP327_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL1725157.1"
                     /translation="MKDIFIQDYEKTKKEILTEYDKSTFTENWQENETWEISFTIVKT
                     KFNELAFDLVDYENSVFFNGQEFIVKQMGVSAEGAAIAKTVTATHIYYTMQDGFQYDT
                     ITGTRSINQLLAHVFKPDNRGFTWNVVDPNKKFLPVEQENFGNGNYLKLVEEILKDYD
                     AIVIPDNKNLTFFPRSEYGKKTEEQIRYKYNTDSVKFDIDTLNLKTQIKGFGKKKEDD
                     TYYFTPITYTSKRSEKWGIRVQSPVSDDRYTVSGNMLERLKQDLQDYPTITGTVTMKW
                     RVEPNKGDYVAFVYEPLGVNTYIQVVGIKTYPAIPNKPPEITLSNTKKTMTSILAEMA
                     KKGVI"
     gene            16438..17811
                     /locus_tag="LP327_001023"
     CDS             16438..17811
                     /locus_tag="LP327_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725158.1"
                     /translation="MELEKLKNNRISNEWKQTFNDNVDYLENLEKNLDEQHKSTNSRI
                     DNLVLHSGGDSPNEVVDARINAEGTIYPTLYSRLLALDNLFNLNYTELKTRQDNQQGQ
                     LNQLNVSVGTLMGAYGETLDLYVAKTGSDQSGDGTEKNPFLTIQAAVNQIPLLTSSRV
                     TILIGDGVYLEDVVIRNLKAVSITLRSRQSVTDVTSDLSVKVRSISFISSLGYQQVNG
                     IEFVDQVNISGQLKCAIYSEQSSYLAVWNCRFAETTYGKSNRCLFATGGSKIATNNNY
                     YLNQNCIAEARNLADINIDPSDQGTGNDYGIIADNGTARIKVVGSKVKANRIAEVRNQ
                     GNVVTGKIIRQITNDDISDRDNITNVNGTIKREGDTVTIAIKYECNNYPSDASNTRNV
                     ILVPAGFQRDQSYPAYHPLALYRNETQPAGARAGLTQASRVVAYSGNGSSYISGTWVT
                     NDPIPNI"
     gene            17827..20019
                     /locus_tag="LP327_001024"
     CDS             17827..20019
                     /locus_tag="LP327_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBL1725159.1"
                     /translation="MFKINESIIVIQAEATNHNNTDVVFWSHDRGTAKLRMKLVRKNG
                     VPQSLPEGTTVPIRLMFRSATAEGGYGKHDYLATIEDRVNGIVSIVLEDNILGYVGKV
                     EGSVYIDFPNDRSLDTAGRFTFYIKRSPIDDSTPELEDYYFNGFSQTIDKIEKILADG
                     KLEIEQKIAESETQIDAKVKDTNDKITKANQDVATLNTNIDKANDRIDQTNQQIGDLG
                     KLKKMYSNSIDFGDYDYSGRPNLFLNLDFSKLSRNASAITEPPAYVKDGGSHFVLDFS
                     DPSANGVDRSVYIPAVGRLEKGATYIATVPIMISDDFSTDYGASPIYPYCVLTNNVAT
                     RVTSLSPDNSCRGKWGLIKKTFTVPANMSDGEFTYFQVISTKTQVGKLYIGYDIKIEK
                     VTSTSDTATPYQPNLLDAPYYLSKVALGENIADPTVKFPITSSSDGIYSKNAPEDFVL
                     GETYTVTIGATKPASQTIRVYLAQKQFSDFKPVEGLVDTWVATVSITQLGENAKGVYL
                     FQTPNTSLGSCTIKWLKIEKGNTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEK
                     GLNDSVSLTEPETVLGLKNFKDGAQIDGVDVATVKNLGSVLQSGSGLTTIQSDKQSAF
                     DEFSYEFERIGDRVFFHARVKTNSTTAVASHVNLLEIPLGFQFPIGWTIVGIPLSVVQ
                     WTTPQGEISALAITDLTGKQVVKFATNRVGNHYISGEWRTADSYPETK"
     gene            20031..20477
                     /locus_tag="LP327_001025"
     CDS             20031..20477
                     /locus_tag="LP327_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725160.1"
                     /translation="MKDIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGFRADGEPL
                     TIADQMFDPKLNQWIVLANALDHNDLNNLKAMYEALEHENGNLKQLNAELMLNDVAIK
                     QENTALKEKADSLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            20479..20616
                     /locus_tag="LP327_001026"
     CDS             20479..20616
                     /locus_tag="LP327_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL1725161.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20654..20947
                     /locus_tag="LP327_001027"
     CDS             20654..20947
                     /locus_tag="LP327_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725162.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20971..21168
                     /locus_tag="LP327_001028"
     CDS             20971..21168
                     /locus_tag="LP327_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL1725163.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21179..22198
                     /locus_tag="LP327_001029"
     CDS             21179..22198
                     /locus_tag="LP327_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL1725164.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            22388..22461
                     /locus_tag="LP327_001030"
     tRNA            22388..22461
                     /locus_tag="LP327_001030"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22422..22424,aa:Ile,seq:cat)
     gene            complement(22645..22717)
                     /locus_tag="LP327_001031"
     tRNA            complement(22645..22717)
                     /locus_tag="LP327_001031"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22682..22684),aa:Lys,seq:ctt)
     gene            23098..23667
                     /locus_tag="LP327_001032"
     CDS             23098..23667
                     /locus_tag="LP327_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBL1725165.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            23654..24514
                     /locus_tag="LP327_001033"
     CDS             23654..24514
                     /locus_tag="LP327_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL1725166.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            24704..25408
                     /locus_tag="LP327_001034"
     CDS             24704..25408
                     /locus_tag="LP327_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1725167.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            25413..27248
                     /gene="walK"
                     /locus_tag="LP327_001035"
     CDS             25413..27248
                     /gene="walK"
                     /locus_tag="LP327_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBL1725168.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            27245..28561
                     /locus_tag="LP327_001036"
     CDS             27245..28561
                     /locus_tag="LP327_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725169.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQNPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            28562..29431
                     /locus_tag="LP327_001037"
     CDS             28562..29431
                     /locus_tag="LP327_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBL1725170.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            29486..30295
                     /locus_tag="LP327_001038"
     CDS             29486..30295
                     /locus_tag="LP327_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL1725171.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(30398..31027)
                     /locus_tag="LP327_001039"
     CDS             complement(30398..31027)
                     /locus_tag="LP327_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBL1725172.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            31197..32489
                     /locus_tag="LP327_001040"
     CDS             31197..32489
                     /locus_tag="LP327_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL1725173.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(32565..33227)
                     /locus_tag="LP327_001041"
     CDS             complement(32565..33227)
                     /locus_tag="LP327_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL1725174.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            33452..33736
                     /gene="groES"
                     /locus_tag="LP327_001042"
     CDS             33452..33736
                     /gene="groES"
                     /locus_tag="LP327_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBL1725175.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            33787..35412
                     /gene="groL"
                     /locus_tag="LP327_001043"
     CDS             33787..35412
                     /gene="groL"
                     /locus_tag="LP327_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBL1725176.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT        12113 a         6198 c         7825 g         9578 t
ORIGIN      
        1 aatgggcaaa ataaaaaata gtagcagcca agaataattt tatagtgtca ctgtggcgga
       61 agcagagaga cgcaaaggtg gaggtaggaa gcgtatggcg cactatcggg accttgtaca
      121 aaactagaaa ggactagtac gtgagtggtg cgattccact ccagtgactt taagcaaccg
      181 aggggggtat gaactcgtgt ggtgcgagcc ctagggagga acaggataac cgcctgtgtg
      241 taggttgcta ttacatatta gatcactcgt tgagtgatct ttttattttg cgcaaaggag
      301 gtaacaacaa tgtatagacc acaatactta gaacagaagt atgaagtaat cactgtgcaa
      361 aatggtaacg gtgagatagt acgaaagtat agaagaccaa taaagagcga tacatataaa
      421 cgaaaggaaa acaatgaagt tatttctttt cgcagaagga gaaaagccaa atgagaaact
      481 actggtatat atcgctaacg aatgaatatc ctcgaaccat tgatgattgt tcagtgcgtg
      541 ttgtgcgttc tgtacaaatc aaagggaagt actctattgt cgaaatgcta agagaagcaa
      601 caccaaaaga agtggataaa tgcaaacttg tttattgcgg tcatggctat tggaaagacg
      661 agtatattca acaaaatatt gagagataca tcaaatgaat gataactatg attacatcaa
      721 gttgttagca agtaattatt agatgttttt aggaggaaaa ctatgaaatt aaaagattat
      781 atcagagaag ggtataacat tgtaacttca ccaaatctag cttacaaaat tcaagaagat
      841 tatcccaatg ctttagtgtt tactgataga gctttagatg ccattcctat agggaagtta
      901 ttagtagatc gtttttatag caatagtcca gctgttctta gagctaagcc acttcaaaac
      961 gcttatgcta ttgaacgctt ttcttgtgag tttactggtt ataagatagt agtaccaata
     1021 atgacagaag agacaaaaag aaaagtaatg caacagacca aaaagaggat tgatgaattg
     1081 gcgttagatg atagaagtaa taagaatctt ctagaaataa aactgaagga tactgactca
     1141 gtaccagaag tttattataa aggtcaacga atggatgaga tgccgagagg tcttattgat
     1201 attacttacc attggcacac ggaagacgat gagtaccaag tgcccagagg agttaacgac
     1261 atcactattg agtattatga ttcacctaat aacaagtact tagatagaaa aatcattggg
     1321 cacaagagag atatgtaaat gaaagaagct agacctagag acgagataga caaactatac
     1381 aagaccaaac gatggcgaga cctaaggcaa gtagtaatag ctagggactt cggcatgtgc
     1441 caagagtgca agcgtcgagg gcggaacaca aggggcacga tcatccatca catagtcgag
     1501 gcgagggaag acctgtcact gttctggtcc gtagataacc ttgaatgtat ctgtgtagct
     1561 tgtcacaata gagagcatcc agagagatca ggcgggaaga agaaaccaaa acctaaatca
     1621 catatcgtta aaatgtattc aactcctgaa agataagttt gcagcgaaat gaaggtaagc
     1681 ccccctactc taaaagatta aagagtaagg cttgagaaga acggtgctgt ccttccttcg
     1741 taaaaagacc gcttttcaag ttttttggag aaaaaggaaa aagccgacca atttaagccg
     1801 gctttggacg aagctatttc ttagtccatt tgtttccttt ttgagaagta ggaggtaatc
     1861 ggtcgcctgg atcaattttt acttctcgtc cgccttggac atttccacca cgaggaccca
     1921 cttctttata ggttcctttt ggtttgttgt cttcgccggg tttatagagt tctcccatag
     1981 gaatccctcc ttaacaaatt tcggcacagc actgccgata acctaattat aaggattgtg
     2041 ataacgattt caatctatct tttgaaagaa ggtgatatta tgccgcaacc agcgaagagt
     2101 gcaaagttac aattattaaa tggaaaccca aacaagaaga ataccgacga actccgtaag
     2161 cgagcggctg cagaagacaa attaaaaatg gctactgaca aaatcaaacc gccgttatgg
     2221 ctagattcgc taggaaagga tacctttgag tttatcgctg atgaattgct gtctgtggat
     2281 ttaatcagta atccggacgt ccatacaatg gctctctact ccaattggta ttcgcaatac
     2341 gtttctttag aaaaacagct tcgaaaacta caacgagagt acaagttgaa ctatgcgctt
     2401 gcgaaaaagg aggcagaggc gagaggtgag ccgtttaatg aacctaatga attaattggt
     2461 aatccgctct ctcggcagat ggatacagcg tctcggaatc tccgttcttt tggcgctgat
     2521 ttaggactat caccagcagc cagagctaaa ttagctatta agatggctga tgatggtggt
     2581 gatgacgatg acgacttcta atattttgga tatgtcttac acagaacgtg tggactattg
     2641 gcaaagctat cttgaggagc aagcttcttg gggtggcttt ttaaaatgtc catatccgga
     2701 attgttaact acttggtatg cggaacgatt aatcgatgga agcataccag ccagtaaaga
     2761 aaatattcaa gctgctaaac ggcatatgcg tgatttgcag cgccaaggaa cagatgattt
     2821 tccttggatc tttgacgaag aaaaaggtca ccggcctatt agatatatcg aaaaaaaatg
     2881 taaaccaact gaaggcgact ttggttcgtt tgttttgcaa ccttggcagc atttcataat
     2941 tggatccatg tatggatggg tacatcgtga tacaggagag cgtcgcttcc gcgaggctct
     3001 tatttttgtt ggacgtaaaa acgggaagac aagtcttatc tcgggccttt ccacatacat
     3061 ggtcgcttat gatgatgaac aaggcgccaa cgtttacgta ttggcaaatg ctcgtgatca
     3121 agcaagcttg ttgtttgata aggccgcaga aatggtcaaa caatcgccgg cgctctttaa
     3181 gaaatttggt aagcctaaac gatcaagtat taattatgct cccgcctttt ctaaaatgga
     3241 accacgcgcc tcagatagcc ggaaattgga tgggctaaac actcattttg gtatttttga
     3301 cgagatccac gagtttacga attacaagct gatcaacgtt atcaagaaat caagaggaac
     3361 cagaaaacag cctctgatag tttatatcac aactgctgga tatgtattag acggtccgtt
     3421 gatgtcttat tttgagcaag gtgtggactg tttggaacat ttggaagatg acatcgatga
     3481 acggactttt tattatctgg caaaacttga cagtgcggaa gaggctgatg acccaagatt
     3541 atggatcaaa gccaatccga atatttgtct aatgaatttt gttggcatgc tagatgacta
     3601 tgttaaggat aaaaaagatc caaaagaata tgctgactgg atcactaagc aatttaactt
     3661 gttttccgat attgatgagc tgtcatttgt cgatatgcct accattaaac gaaacaataa
     3721 aaccatcgat attgaaacgc tcaaaggtaa gaagtgtgtc ggtggttttg acttgtccga
     3781 aacggaagac tttaccgcag ccgttttaga atttccgctt gaaacaggcg aggtattcat
     3841 tttgcaacac acatggatcc cacaagctag atttgatcga gataacaatc aagagcgtat
     3901 caaagcgtgg gagaaggtgg gagatttaac gattattcct ggtgattacg tcaattatga
     3961 atacgtctta aattggtttg tagaaaattc gaaaatctat gacattgtaa aaatcaatta
     4021 tgacaaggcc aaggcgctac gattaaataa agaactagaa aatgcaggat ttgaaaccaa
     4081 cgagattagg caagggtttc tatcattagg cgggccaatg caaaacttca aggaaatgct
     4141 attggacggt aaggtgattt tcaacaattc caagctttac agatggtatc tatccaacgt
     4201 caagctggtg atggatcgca actcaaactg gatgccgtct aagcagtcca agagtagaaa
     4261 aatagatggt tttgcagcaa gtttgaacag ccacgccgaa gtgttgaata tgttggttaa
     4321 tcctgtcgga accgggaaag taacctatta ctcgatttcc gatttaatga atatgtaaga
     4381 aaggtgtgga ggaatgagta ttttagatcg tttgcgttct tttggccgag caaagccgaa
     4441 agcgagcaaa caagagtatt ttttgaatga cccgggattg ataccgtatt tagtcggaaa
     4501 agatgaaata tcagaaggga ttttttccgt aattagccgt gtatcgaacg tttttgcgtc
     4561 tctccctctc aaaatgatag atgtggagtt tggccaaccg gacgactgtc ctgcatacaa
     4621 cttgttgagc gaaggccctc gatattttac aaagtttgat tttttccggg acgtggaagt
     4681 tttgagaaac taccaaggga atgcgtatgt gcagattttc cgaaatatca atggagaagt
     4741 agcagatatg gcgttagtaa aacctggtgc ttgccatcca gtgattgata tggatagcgg
     4801 ggagctttac taccaagtaa ctgcgactga caaaggcagt tacaagcaag ttatctatgt
     4861 acattacatg gaaatgctcc actttaagca acctaggttt ggcggcttgg aaggtacaga
     4921 ccccacaaaa gtattaacga ataccctcgg atatgatcga gaagtccgaa aaatctcttt
     4981 aagtcagctt aaaggaagta atgaagggct aaaagttaag tttgctagca atatggatga
     5041 agaagctaaa aaagctacag ttaaaaacat tgctgatttt tatcgacaaa acggtggact
     5101 acttgtggaa gaaaacggtg tagaaatcga acgtttacaa cgagagctgg tagacagcaa
     5161 gcttttagat actgataaaa tatctcgctc cagaatcgcg atggtctaca acgtgccgga
     5221 acatttcatc gggaataacc agtcgagtta ctcttcacag gaacagctca atatggagtt
     5281 tttgacatac aatctagtac cgaccgttaa tcaatatgaa gcggaactaa ataagaaaac
     5341 actatcgaga gctgaaaaag ctaagggtta tcgatacaag tttaatatcg caagtttgct
     5401 aagagctgat acacaggcca gagggcagtt ttaccagatt atgcgccgag gtggagcata
     5461 ttctgccaat gatgttcgcc gctttgagga cttgcagcct ataaataaaa ctggtatgga
     5521 tgattaccat atttccggag acctatatcc aatcgatatg gatccaacat taagaaaaac
     5581 aacctcgtct aaaagcgtag ccgaaaacgg ttaggctttt ttagtttgca ccgaagggag
     5641 gtgaaagtat gaaaaaagtg acgttaagcg gcgatgtcgt ggataacgat accgcgtggc
     5701 tttatgactg gtttgggatc aattgtatct caccagggaa aatttctgcc gctcttacag
     5761 aagcagcggg ggatgaagta gaacttgata tctcatcgaa cggtggggat gtcctagcgg
     5821 caagcgaaat atataccgct atccgctcct atccagggaa ggtatctgga aatgttgtga
     5881 gcattgcggc aagtgctgcg agtgtaattg cttgtgcttg cgaaccgctt agaatctcac
     5941 ctacggcaca catcatgatt cataacgcat gggtgaccac tagtggcaac gctgaggaat
     6001 taaaagccga tgcagaaatg ttaagcagtg tggatgagtc tattgttaat gcttacgaga
     6061 tcaaaacagg actcgatcgg aaaaaacttg ctgatttaat ggcgaaagat acttggttaa
     6121 atgctcaaac agcagtagcg gaaggttttg cggatgaaat tatgtttgca gaagcaccag
     6181 taacggtact caatgcctct caaccggtta ttccaaaaaa cgcagtaact aagttgaaaa
     6241 atttaataat caaagcggaa gcaccgcaag aagaaacact cttgcaaaaa aaactaaaag
     6301 ccttaaatgg agggaaaaac gaatgaattt agaacaatta aaaaatgcgt gggtcgaggc
     6361 gggaagtaaa gtctctgatt taaacgcgca actcaatgca gcattggttg acgatgaaaa
     6421 aacagaagaa gatgtagtaa gtttgcaagc acaagtaaaa gcagcacggg ctaaacggga
     6481 cggattgaaa gagcaagtgg caaatatgga agccgaacaa gtcttaaacg tcaaaaaaga
     6541 accattagat aaaaaagatg aaaacttgaa aaacaagttt atcaaagact ttaaagcgat
     6601 ggtcaatggt gatcctgcta ttatggctac tttgacatct aatacggatg aatctggtaa
     6661 tgctatcgga ttgactattc ctgtagatgt gcaaacgact attcatactt tggttcgtcg
     6721 gtttgactct ttgcaagaat acgtaaacgt tgaaaaagtg accactgcta gcggttctcg
     6781 ggtttatgaa aaatggtctg atattaaacc actgaccgct ttggatactg aagacggtga
     6841 aatcccagca aatgacaatc ctgcacttta cttaattaaa tacttgatca aacgctatgc
     6901 aggtatttcc acagtaacta acagcttgct aaaagatacc gccgaaaaca ttttggcatg
     6961 gttgtctaaa tggatcgcga aaaaagtagt tgttactcgc aatacaaaaa tcttggcagc
     7021 tattgatgga atcaaagcgg cacaaaagaa agatgttaca gatgttgatg gaattaaaga
     7081 tatcgtaaac gtccaacttg atccagctat cgaagctaca tccatgttta ttacaaacca
     7141 agatggcttc aatgtattag ataaagtgaa acgtactgat ggatcttact tgttgcaaaa
     7201 agacgtaact tctgcaactg gatatacttt cttgggtaaa ccaatcaaga aaattgcttc
     7261 tcgtttcttg ccaaataaag ggacacaagc tactcctaaa tatccactgt acattggtga
     7321 tctgaaagaa gccgttacat tgtatgatcg cgaaaacatg agcttgctga caacgaatat
     7381 tggtggtgga gcttttgaaa cagacaccac taaagtacgc gtcatcgatc gcttcgatgt
     7441 gcaactagtt gatgatgaag cggttgtttt ggctacattt acaactattg cgaacgagac
     7501 accagcggaa ggttaaggag ctgattcctt atgattcttg atcctaaaat ggatttaggc
     7561 gaaatcaaaa acgcactaaa gattgatacc gatgatgacg atgtggaagt aagccgtgcg
     7621 gtacaagctg caattgcata cattagaggg gctatcggga atgataagcc ctctttttat
     7681 aagcaagaaa atgaaacggt tgatctgatt aatttagcta tcctgcaatt agcagatcat
     7741 tattacaacg ctcgatcagc aaccgtgagt gggaacttgc gagagtacga tttaggtttt
     7801 acaagcctaa tcttgcaact caaagcaagt tatttgcttt ttgtggagga ggagtagcgt
     7861 atgcccctta ttcaaacagg aaatttaaat caacgcatca agtttgtccg agatacgacg
     7921 gttaaggatg aggacgggca agttgtcccg acttctacaa ccattcttac ttgctgggca
     7981 agtgtgcaga cacaacgcct gaacgatatt aagacgtcga ttggtacggc tttggaagga
     8041 acactgacgt tcattatccg ctaccaacaa aaatcagagc taaccaatga tatgaaagtg
     8101 cgttggaatg gaaaaacgtt tgaaatcatc acaattacaa agggcgagtt tacaaaagac
     8161 ttcacgacaa tcattgcaaa agaggtttca aaatgagtgt agaagtcgat gcaaccgaag
     8221 tgtacaaagc gcttagggaa gtaaaagcaa acgttcaacg agtggaaagc ccagcactta
     8281 gaaaggctgg ggagtacgct caagaaaagt tacgacaaaa cacaccttac tgggatggaa
     8341 cgaagtcaaa cggtaaacgt ggttcgtata tgcaagaaca tgctaagaac catgtggtta
     8401 caagctcggt aaaaaacgga ttgatagaag tcggctatga caaagatgtt tcttggcgga
     8461 tgcactttat cgagtttgga acaatcaaac aacgtccaaa aggtttcgta caaaaaacac
     8521 aaaagcaaat cgaaaaacaa gtaacacaaa tcattgctga cgaagtaaaa aggaggctag
     8581 gactttgaaa acggcagtat cacaagtcta ttcaattctg aatagcaatg aaaaaacaaa
     8641 gaacattgat ttttacacca atagtgttcc ggaatcggct caaacagtac ctagccttcc
     8701 agttggcaga attacagaga tatccggcaa ctatgaagat tttgcaagca acaatccttt
     8761 gaccattcaa tttaacgtac aggtagatgt atgggtgtca accatgaaag aggttgatgc
     8821 cttttatttt gcccttgatg aggttatgag ggggaatggt tggcaatgcg catacacgga
     8881 acaaacagat gacgaggatt tggaaggtgc aaagcggatt atcaaacgat atgtagcaaa
     8941 tatttcacta aattaaaagg agagaaaata gatggcaaca gtaggatttg agagcgtcat
     9001 ttttggcgta aaaacaggtg caggcggcac tctaaaagaa ttagtagctg ataagtcgaa
     9061 aggtggagcg atcgaagcta aaattactgg attaggcgca acttctaaca caacacacgc
     9121 ttcaaacgta ccgttcttca ttgcaagtaa aggggtttcg tcgccaaaag ttacgcttga
     9181 cgtggcagac ttaatggata acggcattta cagcgaaatc attggtgcta aaaccgtgga
     9241 tggtgtaaat gtaattggtt cagaaactga agcaccttac gtgtcggtag tcatggttac
     9301 agcgaacaaa gaaggaaaac gcttattcat gggattgaca aaaggaaaat tcagtcatcc
     9361 agatatcgac atgaaaacag ctgaagacaa aggggtagaa ttgcaaaccg attctatcga
     9421 aggggaattc atttctgatg gacgtggcta tgtatttatg actgccgttg aatcaacaga
     9481 tatgacctta caaaaattca aggacttggt aaataacaaa gcggggaagt agttaaccct
     9541 gcatctacac caatgacaga tacaggaaca ccaaaagaac cagaaccaaa aattgataca
     9601 caaggttagc cacttttggc tagccttatt ttttgtaaaa acaaggagga aaacaaatga
     9661 ttgaattgca attgaaactt gacggaaaga aaaaaatttt caaacaacaa gatatttccg
     9721 cacgtgcaat gcgtgagtgt atcaaatttt acgagaaagc ggaaaaagca gacctaactg
     9781 atttagaagc aattgattca atgattgcaa ttacagcaga tattttccaa gatccagcag
     9841 ttacatttga tgctatttta gacggtttga ctgcgagcga gttagtaccg gcattagaaa
     9901 gtgtttttga acaaatcaat gaactgggaa acaatgaaaa aaagcagatg gcgagcaaaa
     9961 agagataagt ttttctgaag ctaggaaagc aatggatcaa atctacaaag atttaatcga
    10021 agcaggttgg acgatgagag atgtggacga agccgactat cattatttgt tacacctttt
    10081 tggagaagtg gagaatggcg aagaatatgt agatggtgct gatttcatca aacaattttt
    10141 atcggctgaa gacttggtaa aacttgagga aggaggtaaa taatggcagg aaaaggacaa
    10201 ccggcaggaa atatcaagct agggattagt ttagatagca ctaattttgg taacacgctg
    10261 gacgaaatca atgcgaaagt caaacaagct gagtcgaata tgcgtgccaa tctaaaggct
    10321 tatgattcag caggacgttc atacgaagca cttagtcaaa agacgaaaga cttatctacg
    10381 gttatggaag ggcaaaacgc caaagtaaga gaattaacaa agcgccgtga tgaagcgatt
    10441 agcaagtatg gcgaggaatc gaaacaagtt gctaacctta acacacagat aaacaatgct
    10501 accgcaaaat ataatgctta cagtcgccag ttgaacgaca caaaaaaaga attggtgtat
    10561 tccaaaacag ccgtcaatga tttatctaat gaaatcaaag aaaatgaacg acaaatgaac
    10621 gccgaagtaa aagcgttgaa gtctgcaggt aacgaagcag gagcttttga agcaaaacaa
    10681 aaagggctag ccaaacaaac ggaattatcc gagaaagcta tcgaagaaca gcgcaaagtt
    10741 gtgaaactga tggctgatga gtttggcgat tcagcaaatg aaaccgagga tgcaaaaagg
    10801 gcattagaaa agttagaacg acaaagccaa atatccagta gacaattaga agcactcaaa
    10861 agctccagcg atcaatcagg aaaaaaaata gaagattttg gcgacaagtc cacaaggtca
    10921 gctaggaaac tggacgggct aaaagacaaa ttaggctcgc taaaaagcgc attttcgttt
    10981 ggtacagttg ctggattagc ttcacaagca gttagtggtg ttgttaacag tttcatgggg
    11041 ttgacagatg aagcagtaga ggcttccgat tcaatggaca agtttgttca aacaatgcaa
    11101 tttgctggta tagataattc aaaaattgaa gaatctaaaa cagtgatgaa agattacgcc
    11161 gaccaaacgg tttatgaact aggcgacgta atgaatacga ctgcacagtt ggcagccaac
    11221 ggtgtgaaag actttgatgg tctaacgcag gcaatcggta atgtcaatgc cgtttcaggt
    11281 ggtaacgcag atacgttcaa atcggtagca atggcaatga cacaaactgt cggtgctggc
    11341 aaattaacaa ctgaaaactt taatcagatt gcggatgcca ttccgggcgc ttcagggaaa
    11401 attcaacaag cattgaaaga aatgggcgct tatactgacg gagatttccg caatgctatg
    11461 gcaaacggag aaatctctgc cgaagaattg aataaagcat tcatgcagtt aggtatgact
    11521 gatgtagcta aaaaggcagc aggttccaca gctacgattg agggggcggt cggaaatctg
    11581 caagccagcg ccgtaaatat gattaatgaa ataattaatg ctttcggcaa aggcaatatc
    11641 acttctttga tcagtgacat aggcaaagga attgacaact taacaaccaa aatcaagcca
    11701 gcaatggaaa gcataaaaaa cgtcgctcaa cctgtatttg acatgttgaa aaaaggggct
    11761 gacatagcaa cacttgcttt tgggacgctg tttggaacgt tagatggaga tcaagcaaag
    11821 caaggctttg atattctaac taaaattttc ccaccagaaa tggttacttt cgcacaagat
    11881 accatagaaa aaataaagaa cgcggttaag acattgtttg acgtattcaa aggtaacgca
    11941 gacatggaag atgttctagc taaattagga ttttctccag aaacaatttc taagataaaa
    12001 gatgttttcg acaatctcaa aacatatttt gatggtacta tgacaaccat aaaaaacgga
    12061 atagaaattg gatggcaaat tgcaaaagga attttcgata ctttgtcgcc atatataatg
    12121 cccattatag aaaagatagg cggagctttt tctaaaattg gaaaatcgat gaccacattt
    12181 tggaatgaaa atggtaaaca gattactgaa gcaatcaaga acttttttac gtttatacag
    12241 cccgttgtaa aaatcgttat ggatttagtc atggggttca ttgacaacat aataggttta
    12301 gtcgaaggga ttatgaacgt cattcaaggt gctatcaaaa tcttcactgg tttatttaca
    12361 ggcgattttt ctaagatgtg ggaaggagta aaacaattgt tttggggttc tatccaagca
    12421 gtatggaact ggattcaaat actattcttc aaacggattt tagaaggagt aaaaggtttg
    12481 tggactggtt tttcaggttc aataaaaggg ctatgggaaa gcactaaaac tttctttact
    12541 gaaggtattt ccaaaacttg ggataagcta gtcaattggg taatgaattt gttgggcaaa
    12601 gttggcaacc tgaaaaccac gtttggcaat ttcatccaaa acatgtggaa cagcgtgaaa
    12661 aacttcttca gcaatggcgt tggagacact tggaataagg tagttggctg ggtaaaaaac
    12721 attttcaaca aagcaattga attgaagaac aatgtttctg atgcaatcgg taacctgtgg
    12781 aaaggtatca aagacacatt ccgtagaggt attgatacgg tattcaattg gttttcagaa
    12841 ctacccaaca agatgaagga tgccattatt ggtggtaaag atgcccttgt tgatgcgttc
    12901 aaaagtattt tcaacgcagc acttaaagcg ataggtaaac cagttaacgc gatcatcaat
    12961 ggagcttcat gggtactaga aaaactgggt gctgacaaac tcaaagaatg ggaagtacca
    13021 caatacgcaa aaggtacacc agccggtgga cacccaatta atggaccaat gatggtcaat
    13081 gatggacgtg gagcagaaac agttatcaca ccagatggta gagcattcat acctaaagga
    13141 cgtaacgtag tattgaatgc accaaaagga acacatgtct tgacagcaga agaaacagcc
    13201 caactccaag gttcaaaagc accaaaatac cgttacaaaa aaggtactaa cttctttggt
    13261 aatctgtgga atagtgtgaa aaatgtggct ggtaatgtag gaaacacact aaaaaacgta
    13321 gtcggcgatg tatgggattt tgtaacagac ccgggagcgt tggctaggaa agtgttaaat
    13381 ggtcttggcg tactggaagg gcttgtcaaa tatcctttag atgttggtaa aggtattcta
    13441 agcaaggcta ccgaagcatt gacgaacaaa atcacagaac tattcagcag tggcagttta
    13501 gacacttcaa tgggcatgca aggcgtttac aaatacttgg cggacgttgc agttgcagta
    13561 atgaagaagt ttccaggctt tcaagtaaca tcaggttatc gtgaaggcga tccatactca
    13621 cacggaaagc acaatgcaat tgatattgcg ctaccgggag tcgtgaatgg ttcccctaga
    13681 tatacagaag cagccaatta cgcatttgag aagtttgcaa acaaaatcgg ctatgttatc
    13741 acaaatggta aggttcgtga ccgttcagga caatcaggta caggtgtgca tgatgattgg
    13801 cggacatggc ctgatggtga ccactacgac cacgtgcatt tgaacggtgt aagagatccg
    13861 cagggcggac ttgttagcgg tggcgatagc gttggtggga gtggcgtaga acgctggcga
    13921 aatgtagcaa ttagagcgtt gaaaatgact gggcaataca gtactgcaaa cttgaacgca
    13981 ttactaaatc aaatgcgtac agagtcaaat ggtaacccta gagcaattaa caactgggat
    14041 attaacgcta aaaacggcac accttctaaa ggattactac aagtgattga cccaacattc
    14101 agacagtatg caatgcctgg attcaacagt aatatctatg acccactatc taacatctta
    14161 gcttccatca gatacgcgtt gtcaagatat ggctcactaa gaaatgctta tcgtggtcat
    14221 ggttacgcaa acggtggaat tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac
    14281 aagccagaaa ttattattcc gttagataag gctaaacgat caagagcgat gcagttgctt
    14341 gcgattgctc aagataagtt aggagtaaaa ccaaaaagcg taaataatag tagcgattcg
    14401 agcggaacgt tagaaacatt agtttcactg atgattcagc agaataactt gctatctaaa
    14461 cttttagcaa aagacacaag tgtcaaactt gatggtaaag caattgcaga caatacaaat
    14521 ggatacttag gtaaccagtt gaaacgttcg ctatatacaa caggttagga gggataaagt
    14581 gaatggctat ttaatcgatt ttcgcttcat aaaaaatcaa gaggtagtat ctctaaaaga
    14641 aaaattgggc atagagtgta tttcttttgt acgaaaagca ccacaactaa acgtagaata
    14701 ccaagaattt tcagggtcaa acggttcgag agaagtcgaa aaaagtttca aatcgttcac
    14761 tatcgaagtg gaattttatg ctgaattcaa aaatatgtat gactatcaac taaaagaaac
    14821 tgaattatat gcgtttctat tcgatgacga aggatattat gtttttacag atagagaacc
    14881 gggcaaaaaa tactttgtcc gtcctaactc agtagaagtg aatgaagttg gtctaagata
    14941 cgcaacttat aagacgactt tcactgtttt tagaggttgt tccgaatcga tggcttccac
    15001 gttatcggag ttttcactgt ctaatgaatg gcaattttca caaggcctag ttgcggaaga
    15061 ttgtaagtat acgcaccgaa ccagtaattt tatcatttat aatgctggcg attttgctat
    15121 tgatccacgt gaacatgctc taaaaatcac tttggaaggt gaatcagaag gcaacgtgac
    15181 tattttcaac aaaacgacag gggaacgatt catctactat ccagagtttt ctacgttgct
    15241 aggccaaact ttgactttag accgtgttta tccaaagttg aacggtgtaa attgcggaat
    15301 tgacacgaat ttaggtttga taacgttagc ggttggaacg aatgaaattg aaatacaaaa
    15361 tgttactaga gtggagtcaa aatgggactt caattttttg tataagtagg tgggaatttg
    15421 aaagatattt ttatccaaga ctacgagaaa acaaaaaaag aaatattgac tgaatacgat
    15481 aaaagtacat ttactgaaaa ttggcaagag aacgaaacgt gggaaatttc gttcactatt
    15541 gtcaaaacaa aattcaatga attggctttt gatttagtcg attacgaaaa ttcagtattt
    15601 ttcaatggac aagagtttat cgtaaaacaa atgggcgttt ctgctgaagg ggcagcaatc
    15661 gcaaaaacag ttacagccac gcacatttac tacaccatgc aagatggctt tcagtacgac
    15721 acaatcacag gaacacgttc tatcaaccaa ctgctagcgc atgttttcaa acctgataac
    15781 cgtggattta catggaatgt tgtagatccg aacaagaagt ttttgccagt tgaacaagaa
    15841 aacttcggga atgggaacta tttgaaactg gttgaagaaa ttttgaaaga ctatgatgcg
    15901 atagtgattc cggacaacaa aaaccttact ttcttccctc gttcagaata tggtaaaaaa
    15961 actgaagaac aaatacgcta caaatacaat accgattccg tgaaatttga tattgatact
    16021 ttgaatttga aaacgcagat aaaaggattt ggcaagaaaa aagaagatga cacttactac
    16081 ttcacgccaa tcacatatac aagtaagcgg tcggaaaaat ggggtatacg tgtccaaagt
    16141 ccagttagtg atgatcgtta caccgtttca gggaacatgc tagaacgttt gaaacaagac
    16201 ttgcaagact atcccacaat cactggcaca gttactatga aatggcgtgt agagcctaat
    16261 aagggcgatt acgtggcgtt tgtctatgag ccgttaggtg tcaataccta cattcaagtc
    16321 gttggaatta agacgtatcc agcgatacca aataagccac cagaaatcac attgagcaac
    16381 acaaagaaaa caatgacgtc gatactcgct gaaatggcga agaaaggagt gatttagttg
    16441 gaattagaaa aattgaagaa taaccgaatt tccaatgagt ggaaacaaac attcaatgac
    16501 aatgtggact acttagaaaa tttggaaaaa aatttagacg agcagcacaa atcaacaaac
    16561 agtcgtatag ataatctcgt gcttcattca ggcggtgatt ctcctaacga agtagtggat
    16621 gctcgaataa atgctgaagg tacgatctat ccaacgcttt attcgagatt attagcattg
    16681 gataaccttt tcaatttgaa ttatacagaa ttaaagacaa gacaagataa tcaacaaggt
    16741 cagctaaatc aactaaatgt ttctgttgga actcttatgg gagcatacgg cgaaacgctc
    16801 gatttatatg ttgctaaaac aggaagtgat caaagtgggg atggtacaga aaaaaatcca
    16861 tttcttacaa ttcaagctgc tgtaaatcaa ataccgttat taactagttc acgagtaact
    16921 attttgattg gcgatggagt ataccttgaa gatgtcgtga ttcgcaatct caaagcagtt
    16981 agcattactt tgcgcagtcg ccaaagtgta acagatgtaa cgtctgactt aagtgttaaa
    17041 gttcgctcca tttcatttat tagctcttta ggttaccaac aagttaatgg aatcgagttt
    17101 gtagatcagg tgaatatttc aggacaatta aagtgtgcaa tttattctga acagtctagt
    17161 tatttagctg tctggaattg tcgttttgct gaaactactt atgggaaatc aaatcgttgt
    17221 ttatttgcca caggaggttc aaaaattgct acaaacaata attactacct aaatcaaaac
    17281 tgtattgcag aagctagaaa tttagctgac attaacattg atccaagtga tcaaggaact
    17341 ggaaatgact atgggataat tgcggataac ggaactgcta gaatcaaagt tgttggttct
    17401 aaggtaaaag cgaatagaat tgctgaagta agaaatcaag ggaatgtcgt tacaggtaaa
    17461 atcattcgcc aaatcactaa tgatgatatt agcgatcgtg acaatattac aaacgttaac
    17521 ggtactatta aacgtgaagg agacacagtc acaatagcaa tcaagtatga atgcaataat
    17581 tatccttctg atgcttctaa cacaagaaat gttattttgg ttcccgctgg atttcaacgt
    17641 gatcaaagtt atcctgctta ccatccgctg gctttatatc gcaatgaaac ccaacctgct
    17701 ggcgcaaggg caggcttaac acaagctagt cgcgtagtcg catactcagg aaatggatca
    17761 tcttatattt caggtacatg ggtaacgaat gacccaatac caaatattta acaaaggagg
    17821 atatagatgt ttaaaattaa tgaatcgatc attgtgatcc aagcagaagc aactaaccac
    17881 aacaatacag atgttgtctt ttggtcgcat gatcgaggaa cagctaagct tcgaatgaag
    17941 ttagttcgga aaaacggcgt tcctcagagt ctcccagaag gaactacggt tcctattcgt
    18001 ctgatgttca gatctgcaac ggcagaaggt ggatatggaa aacatgacta tcttgccacc
    18061 atcgaagatc gcgtgaacgg tattgtgtct atcgtattag aagataatat tttaggatat
    18121 gtcggtaaag tagaaggtag cgtatatatt gattttccaa acgaccgctc gttagataca
    18181 gctggtcgtt ttacttttta tatcaaacgc agtccaatcg atgatagtac gccagagctg
    18241 gaagattatt atttcaatgg tttcagtcag accattgata aaattgagaa aattctagct
    18301 gatggaaagc tagagattga acagaaaatt gcggaatccg aaacgcagat tgatgcgaaa
    18361 gtaaaagaca caaacgacaa aatcacgaaa gccaatcaag atgtcgcaac tctcaatact
    18421 aatattgata aggcaaatga tcgtattgat caaaccaatc agcaaatcgg cgacctcggc
    18481 aagttgaaaa agatgtacag taacagcatc gacttcgggg actatgatta ttcgggaaga
    18541 cctaacttat tcctaaattt agatttttca aaactaagta gaaatgcttc agcgattaca
    18601 gaacctcctg cttatgtcaa agacggcggt tcgcattttg tacttgactt tagcgatccg
    18661 tcagcaaacg gcgtagatag aagcgtatat attccagctg taggacggtt agaaaaaggc
    18721 gctacttata tcgcaactgt gcctataatg attagtgatg atttctcaac agattacggc
    18781 gcttcgccaa tctatccgta ttgtgtatta actaataacg ttgcgacacg tgtcacttct
    18841 ctatctccag ataatagttg taggggcaaa tggggtttaa ttaaaaagac atttactgta
    18901 cctgcaaaca tgtcagacgg agaatttact tattttcagg ttatctcgac taaaacacaa
    18961 gtcggtaaac tttacattgg ttatgatatt aagattgaga aagtaacgtc aacaagtgat
    19021 acggcaactc cttaccagcc aaatttactc gatgcgccgt attatttgag taaggtggct
    19081 ttgggtgaga acattgctga tcctacagtt aaatttccaa tcacaagtag tagcgatgga
    19141 atctattcaa aaaatgcacc agaggatttt gttttaggtg aaacttatac tgtgacgata
    19201 ggggcgacta agcctgcctc acaaactatt cgggtttatt tagctcaaaa acaatttagt
    19261 gactttaaac cagttgaagg attggttgat acatgggtcg ccactgtgtc aataactcag
    19321 ctaggggaaa acgcaaaagg ggtatatctt tttcaaacgc caaatacttc tttaggttca
    19381 tgtaccataa aatggttaaa aattgaaaaa ggcaacactc gaactccgaa tattagtcag
    19441 tttaaatact tcggtgaagg cttgaaagac agcaacaatc ccaatgatta cagttgggat
    19501 gtcacacctg aatatactga aaaaggcttg aatgattctg ttagcttaac cgagcctgaa
    19561 actgttttag ggcttaaaaa tttcaaagat ggggcccaga tcgacggagt agatgtggca
    19621 acagttaaaa acttaggtag tgtgttacag agtggatctg gtttgactac tattcaatca
    19681 gataagcaaa gcgcatttga tgaattttca tacgaattcg aacgtattgg tgatagggta
    19741 ttctttcacg cacgagtaaa aacaaactcg acaacagccg tagcttcaca tgtgaatcta
    19801 ctagaaattc ctttaggatt tcagtttcct attggatgga caattgtagg gattccgttg
    19861 tcggttgtac agtggactac gccacaagga gaaatcagcg ctttagcaat cactgattta
    19921 actggtaaac aagtagttaa atttgcaact aatcgagtag gaaatcatta tatttcagga
    19981 gaatggcgaa cagctgatag ttatccagaa acaaagtagg agggaaataa atgaaagata
    20041 tttggaaata tggacgtact ggcggagagt acgcaggaaa agtattggac gacatgcttg
    20101 tatccgttcc ttacacggat cagccaccgc tcgaaggatt ccgtgctgat ggcgaaccgc
    20161 taacgattgc tgatcagatg tttgatccta aactgaacca atggattgtt ttagcgaacg
    20221 cgttagatca caacgattta aacaatctca aagcgatgta cgaggcactg gaacacgaga
    20281 acggcaatct aaaacagcta aatgccgagc tcatgctaaa cgatgtagca attaaacagg
    20341 aaaatactgc attgaaagaa aaagcggata gtttagcaca aatcaattca aaaacgatgc
    20401 ttgcttcgtt ccaaaatagc aaagacattg cagaaattaa agagcaacta aatccagctt
    20461 caaagggagg tgagtagtat gtttagtttt agcgatgtga aaatgatgta tgattggggc
    20521 tgttttactg acgatcaagt tcgactattc gttccactat gcattacaga cgaagaagca
    20581 gataaaatca ttagcaaaga agagagcgca tcttaattga tgtgcttttt attttgattc
    20641 aaggagttgt cacatgatta atttaggaga atggggaaca atcgcaggat caatcactgc
    20701 gattgtttct ttgattttat tagtaataag accagtcact gcatctttct cgaagattac
    20761 tgaaactctt tcaaaagtaa gtcacaattt agatttgctg actaaagatt tagaatcgag
    20821 caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac
    20881 acatgcagaa aaattggtgg aacacacgca acaaattaaa actttattta gagaaagatc
    20941 tcggtaaaaa aagaaaggag ttaagaagaa atgatcttac ctgataaata ttatcaagtc
    21001 attaaatgga cagttttaac agtattgcca gctgcatctg tattagtagc gacgttaggg
    21061 aaagcatatg gatggaatgg aacagatatg acagttctga ctatcaatgc agtagccacg
    21121 tttttaggtg ttatcactgg tgtgtcggct tataatttga aaaaatagga ggaaacaaat
    21181 gaaaaagaaa attactatta ctgcgatgag cctattaacg gctctttttt tattgccaat
    21241 taatgggttt gcctatacga ttaacaatga atttaatttg ggcccaaacg aaggtagctc
    21301 acaagtagca aataatcagt acattttact gcatgaaacg gctaatgaaa cagcaacagg
    21361 acgcaatgaa gcacagtata tgcaacgttc atggactagt gcttacactg cttatattgt
    21421 gggagacggc ggaattgttt atcaagtcgg tcaacctggt tatgtacagt acggtgctgg
    21481 ttcgtatgct aatgccaaca gtcctgtgca gattgagtta cagcacacac atgacaaagc
    21541 aacgtttgaa aagaactata aagcatacgt tgaactggca agagattcag caaagaaata
    21601 tggtattcca ttaacattgg acactcctta taaccaacca ggaatcaaat cgcatttatg
    21661 ggtaacacaa aatatctggg gagatcatac tgatccgtac ggatatcttt ctgaaatggg
    21721 cgtaagtaaa gaaaaattag catatgattt agctcatgga tttaccgatg aaaatccaac
    21781 gacttcagat gataaaccag tcattgatcc aactagagca ggtgcggcaa atcctacact
    21841 gacagatgga acaaattacg cccacattga tcagtttggg gaaatcgaaa acgcaaactt
    21901 gcatgtggct ggatggcaca ttgctaacta taaatacgag tatattttca ttatggacta
    21961 caatactggg aaagaattag ctcgagtaag agctgatgga atttatagac cagatgtaaa
    22021 ccaagcttat aatacttctg gaaatgttgg ttatcatgta tctttcaata tgcgtaattt
    22081 tcctagtaag aaagtctatg taatgatgcg ggcaacgaat gatccagagg gaaacactaa
    22141 aggcggtgcg caagatttcc atgacaaacg ttggtattta aatattccta aacgataaaa
    22201 atagctcctc gttgaggagc agtacataac tatattgaca actataaaaa ttattcgata
    22261 aaatagtgat gttatcgcat atcttcacta tcacccataa atagtcacac tccaagctat
    22321 gcgataacag gtttgttgcc acacattcta ctggttgatt gtttatggct ttatgtggca
    22381 acaaccagta cccttagctc agttggtcag agcagacggc tcataaccgt ccggtcgtag
    22441 gttcgagtcc tacagggtac atatatacca aaagcactca tcaatttaat tggttgagtg
    22501 cttttttatt gttattaaaa attaaaaaac taaaatacat acccgaattt catacccgaa
    22561 aaataaaaaa tgccaccacg tacactcttt tcgaaactac ctaagtagcc aagaatgtac
    22621 tggtgacgtg ggtttggcac ccaatgggcc gtgaggggct cgaacccgcg acccgctgat
    22681 taagagtcag ctgctctacc aactgagcta acggcccgta caaaaagtac ttaataatag
    22741 tagcactaag aattttaaaa tgcaagacta catttaaaga aaatcagaaa aatctttact
    22801 tccttatagg aaatcattca tagattattc acatttgctc gctattatat aagcatacca
    22861 acaaacaaag ttcttgcaag agaacaaacc ctaacacttt tttcattttt tactcctttc
    22921 ccgccgtctc cccggcgggt ctcctgttct taacgataaa agacaagaag gcttagcctt
    22981 cttgtttttt gttttcatgg aatcccgcta atcaaatcac ctagttaaag caactagatt
    23041 gaatgtttat cataagtctg atatcatgtt tatgaaatga aaaaaggagc ggttatgatg
    23101 gaaaaaatgc gtcagcaatt aatggagtgg gggaaggaac tagaaacatt ccgtctgcct
    23161 cactgggaag aactccctga cttagaactt tatatggacc aagtcagaac gttagttgat
    23221 cgttatcttt cacctgtgat acaaggagaa aagcatccat tgcttacctc ttctatggta
    23281 aacaattacg ttaaactagg tttgatacca gcaccagtta aaaaaagata taacaaagaa
    23341 cacgtagcat ttttacttgc aatcactacg ttgaaacaag tattgactat tcctgaaatc
    23401 aaagaaggga tattgttcca aggaaaaaca gtaggaattc gtgaagccta taatctattt
    23461 tgcgatgaac aagaagcagc tgtgtggatg gtcagccaat tagcacaagg taaatctcat
    23521 ccgcaaaaat tcgaccaggc aacccctgta gaatatattg ctgtaagagc ggcaacttta
    23581 tcgtttgcaa tgaaacttct tgcggaaaaa acgattgttc tagaaaccga atatttaaaa
    23641 gaggagaaaa caaatgaaaa acaataaaat cgcaatttta gtagattcag gtacggatgt
    23701 gccagaagaa ttgataaaaa aatacgagat gtatatgatc ccattgaaaa ttatttataa
    23761 agatcaggta tatacagata aaattgacat tacctctgaa gagatctatc aacgtttgcc
    23821 agaggaaatc cctggtacat ctttgccgga tggtgagtcg atcacagaaa tctttgagca
    23881 aatcaaaaaa gacggttatg aaaaggtact tgcggtaaca atttccagtg gattgagcgg
    23941 gacatataat gtggttcgtt taattgcaca acagcaagaa gaattggaag catatgtact
    24001 agatacgaaa aatattggta ttggtgcagg tttttctgcg atccaagcag caaaatggct
    24061 ggaagaagga gtagaatggg atcaactgat cagtaatctg aatgagttag tcgaaagaac
    24121 aaaagttttc tttaatgttg cgacattgga atatcttcaa aaaggtggac ggatcggttt
    24181 agttgcttct attgtaggaa ccgctttaaa gcttaaccca atcatctcat gtaatgaaga
    24241 agggatttat tacacagtag gaaaagctag gggacgtaaa aaaagtttgg ataaaacggt
    24301 tgcttacgtc aaagaaaaaa tcggtaatgc aaaggtcttc aacttagctg ttgcccatgg
    24361 ggatgcgaaa gaagaagcaa ttgaaatgga agcgagactt aaaagagagt tcccacaagc
    24421 aaaccaaatc tactttggcc agatttctcc ggcattagta gtccacactg gtccgggatt
    24481 attgggtgtc ggtgtacaag ttttagaagg ataaatcaaa aaaaggaaac actgaggtga
    24541 aaatcagtgt tttccttttt tattgaagtt agaacaaaaa ataataaagt attttgcact
    24601 ttcattttaa ttttctcttt tgttttatag gcaaagatga aacaaaagca gaaatacggt
    24661 ataatgagaa ggtatgacaa taatatataa gaggtggctc tttatgaaaa aagttttagt
    24721 tgtcgatgat gagaagccga tttcggatat cgtcaaattc aatttagcca aagaaggcta
    24781 tgatgtttat actgcatatg atggggaaga agccttggaa aaagttgctg aggtagaacc
    24841 tgatttgatt ttacttgatt tgatgctacc taaaatggac ggcttagaag ttgctcgtga
    24901 ggtacgcaaa acttatgata tgccgatcat tatggtaaca gccaaagatt ctgaaattga
    24961 taaagtccta ggcttagagt taggcgccga tgattacgtg accaagccat tttccaaccg
    25021 agaattagtt gcacgtgtca aagcaaatct tcgtcgcggt gccacggctg cgaaggaacc
    25081 agaagaggca gcacctgcgg aattgacgat tggtgatttg acgatccatc cagaagctta
    25141 catggtgacg aaacgaggag aaacaatcga gttaacacat cgtgaattcg aactactctt
    25201 ttacttggcg aagcaccttg gacaagtgat gacaagagaa catttattac aaacggtttg
    25261 gggttatgat tattttggag atgtacgaac agtggatgta actgtacgtc gtttgcgtga
    25321 aaagatcgaa gataatccaa gccatccaaa ttatttagtg acacgtcgtg gtgtcggtta
    25381 ctatttgcgt aacccagaac aggagtaatt ggatgaaaag aaaagtccgc ttttttcgat
    25441 cagtcaattt taagatcgct attaccttta ttctgatttt attgatctcg atcgaaatta
    25501 ttggtgccta ctttatacgt ggtttagaac gttccacgat caatacattt attaaagata
    25561 tgaatcaaac ggtggaatca ttagcaacaa caatcagccc ggaattgaat cgaaaagata
    25621 atgcagatga tgaagaggta aatgcgaata tcaaaagatt tattgaaaac agtgcgacta
    25681 gcgatatcat cgaaatacgg gtcgttgacg aaaaagggat catccgagga acaaccgatg
    25741 tcaatgaaca aagcgctgtg ggaaagaaaa atgattatgt tgatatcaat gatttcacga
    25801 ctaaacgtta tgttgcttta gataacgacg ataaacgggt gaatatcaat gttcaaccga
    25861 tcctgtcccc gaccggcgat gccgttatcg gggctcttta cgtcaaaagt aatatcgaac
    25921 aaaaatattc ggaaatcaat aacacagcag tcattttctt tactgcctca ctaattgcgg
    25981 catttatttc aatgatcgta tctgttttag ttgctcgttc gattacacag ccgatcggtg
    26041 aaatgagaga acaagccatt cgaatcgcta gaggagacta cagtcggaaa gtcaaagtgc
    26101 atggacaaga tgaacttgga cagctggctg ataccttcaa tcagttagct gaacgtattg
    26161 aagaagctca agatacaatg gaagctgaac gcaatcgttt ggatagcgtg ctatcccata
    26221 tgacagacgg cgtcattgcg acagatcgca ggggtaaagt cattacgatc aatgatatgg
    26281 ctgtttcctt gctagatatc aaaaacgagg aagcaatcgg acaatcgatt ttgacacttt
    26341 tagatatcga agaagaatat acgttgcgga aattattaga agatccaaac gaaatggtac
    26401 ttgaccgttc aaccagtaca ttggaaagtg atcagatgat cttgcgagta gacttttcta
    26461 tgatccgtcg cgaatcaggt tttatcagcg gattagtagc tgtcttgcat gatgtgacag
    26521 aacaagaaaa aacagaacgg gaacgtcgtg aattcgtctc taatgtttcc catgaacttc
    26581 gtacaccgtt gacaagtatg cgtagttata tcgaagcatt gagcgaaggt gcgtggaaag
    26641 acgaggaagt agcaccaaat tttttgaagg taacgctaga tgaaacagac cgaatgattc
    26701 gaatgatcaa tgacctctta aatctatctc gcatggatac aggtaatacc cagcttcaat
    26761 tagaatatgt caatttcaac gagttagtga attttgttct tgaccggttt gatatgatgg
    26821 tgggaaatca ggaaaaaaat tataagatcc gtcgtgaatt tacgcaaaga gatctttggg
    26881 tcgaagtaga tacagataaa atcatccaag tagtcgataa tatcatgaat aacgcgatca
    26941 agtattcacc agatggggga acgattactt gtcgcttatt agaaacgcat aataacgtgg
    27001 ttttaagtat caccgatcaa ggattgggga ttcctaaaaa agatctgaac cgagtgttcg
    27061 agcggttcta tcgagtagat aaagcacgtg caagagcaca aggtgggaca ggacttggat
    27121 tagctatttc tagagaagtg attaaggcgc atcgaggcgc tatttgggca gagagcaagg
    27181 aaggaaaagg gtcgactttt tatatctcgc tgccatatga accttatgag gaggaatggt
    27241 gggaatgaga atctctgaaa aagtcgttcg tataggactc attttgatgg ttgcgttaag
    27301 catatacttc tcttatgcaa tctggctgag cccagcagga aaaagttcaa tcaacgtaga
    27361 tgagacaaac actcaaatga tcgagtctca aagcaatcga aaaacttccg aagtctttct
    27421 tccgcttcat gttacatggc accattcggg aatgatccaa gaaaccaaca gtgaaaatct
    27481 cgtgtaccga ttgcaattga tcatcgataa agcaagattt ggaaagcttt ctgaagtagt
    27541 ttcaggagac aaatcacagt ttgaacaggt gaaaaatctg aactctggtt ttgagttggc
    27601 ctataatgcg ccttttttgc taagagaata caaggaagca tttgatttag atttagatgt
    27661 gtcgaacttg gcaaaagaaa gcgaaaatat gtattttact agaatacaat tcgatcaagg
    27721 aaataaaaaa atccgatttt tgaattattc gaaccatctc gtatatgaag caaatatttc
    27781 gttgaattgg acagatatca ataaagaatt aacagcaaaa gacacaaaat ggacagaaat
    27841 gacaacagaa ccatcgacga tcgacacgca atatgctacg aaacattcga tcaaactgaa
    27901 aaagtatagt tatatcctgt cacagcaacc ctatacactt tttagaaatg cttttttcca
    27961 gaacccagat agcgtgaaaa ataatgatga aagcaatgaa ttgatttttt acggtgggaa
    28021 tgaaacaatg tctatccatt cggaaacgca agtagttgat tttcgtggag agctgccaga
    28081 gaaaaaagaa aatgctagtg tattttctga aagtttcccc tatattagca aattaggcag
    28141 ttctttaggg aacctcagat attttgaccg cagtgacaat acgatcgatt atcggatatt
    28201 cgtggaaggc ttcccggttt ttgggacaga cagcagagga cagattggtg tggaagttgg
    28261 cggtgaaacg aatggttctg tgcaagtgaa tatccaaacc agtttgaata cgattcaagt
    28321 gccgatacct tcagacgaag aagtagaact tgcaagtacg aatgaaatca tccagcagtt
    28381 aaaagctaag ggagcagata gcaacaagat tggttcagtg atcgttggct atacatggca
    28441 aaatgtcaaa gaaacgaatc gtgtcgttga tctcttacct gaatggtatg tgaaatataa
    28501 agataactgg tattcagcta acgaccttct ggaaaaatta cctggattgg aggcgaatta
    28561 attggatttc aaaaaaatcg aatggatatt tttcgtcgct tttttagggt tgaatgtgtt
    28621 tttattcagc agttatcatg acgcggtata ccaagaaaac aatgtcattc gctctaatca
    28681 gactgaatca atccaacaac gtctggctag cgacgatatc acttacacgg ggaagatttc
    28741 gtctgagaac aagcaaggct attatcttag cgcggagcag accaatctta gcacttcatt
    28801 agcgaattat cgcaaggctg gtaaaagcgg cttgctggca agtggtgtgt ttttagacgg
    28861 tgatgttttg acgaaatctt tatccagttc agatgctgaa aagaacgtga tcgatccaga
    28921 taaaattagt agttccttgg aagatttttt atctaacaaa gacaatattt tattcggtaa
    28981 tgattatgtg tacacgaaaa acttttctca tttagaagaa gaaccactag aaatccaagc
    29041 aagccaagag tatgaaggca tcccattcaa tgatgataca gcaaaaatcg ttctttcttt
    29101 agaaaaaaac gataatgatt atagcatcac gaaatacatg cagacgcatt tatctgatat
    29161 cgagcagctg cgcgaaaaga cggaactgta tacggaagag gaagcaatca atacattgta
    29221 catcaataac aaaataccaa gaggatcaaa aattttatgg cgtcagctag cttattcctg
    29281 tattctgaaa gtccgggaaa aaaatgtgta tgttcctgtt tggcatgtag cgatcgaaac
    29341 aagcgataaa gtggtccaaa ttgagagtgt caacgctttc agcaatacga tcgtgacaaa
    29401 caatactgta ccaaaagtgg aagatcaata aaaaagcgag tataatgaaa aaagtttaca
    29461 aagtaagaag ggaagtagga gtcaaatggc tgaagaaaaa ccagctttta aaatcagtat
    29521 cctagcaagt ggaagttcgg gtaattcact ttatatcgaa agtgaaaaaa aacggctttt
    29581 ggtcgatgca ggacttagcg gtaaaaaaat cacttccttg atggctcaaa ttgatcggac
    29641 accggaccaa ttagatggaa tacttgtgac gcatgaacat cgagatcata tacatggcgt
    29701 gggtgtcttg gctcgtaagt atcatttgga tgtttttgcc aatcagcaga cttgggaagc
    29761 gatggaacca ctgattggaa atgtaccagt ggaacaaaaa catctatttg agatggggaa
    29821 agtcagaaca ttcggcgata tcgatattga gagtttcggc gtctcccatg acgcagcggc
    29881 tccgcaattt tatcgtttcc ataaagaaaa tcggtcattc gtcatgttga cggacaccgg
    29941 atattgcagt gattatatgc gaggtgtcat tgaaaatgcc gatggttatt tgatggaaag
    30001 taatcatgat cttgagatgt tacgcatggg accttatccg tggaatttaa aacagcgtat
    30061 tttaggagat agaggacatc tttccaacga agacggtgca cttgtcatga ctgatgtgat
    30121 cggtaatcgg accaaacgaa tctatcttgg ccatcttagc aaggaaaaca atatgaaaga
    30181 acttgctcat ttaacaatgg aaaatgtttt gaaagaaaaa gattttggcg ttggacatga
    30241 ttttgaaatc tatgatacag atccagatga agcaactgag ctattttcga tttaaacagt
    30301 tcaatcaata aaaatataaa aagcagaaga taaaatcaat cagggttggt tttatcttct
    30361 atttttattt ttttagcata ttaaaaaaat aaccgtatta tcgactgact acactgatgt
    30421 gctgcttttc atgtttctca tccagtatct catctactaa tgcaatcgcg taatctgcca
    30481 tgctgatcgt actgtcgcca gcagcgtttc tttgtaaata atcatcactt aacaaataag
    30541 tacctgtacg tttgccatgt ggatcaaata aagaagctgg actgatataa gtccaatgta
    30601 gatttttaga ttgtttcaat tttaacaatc cttcgtacat attgaaagct gttggataga
    30661 agggtgcatt tttaggtact tggtcaatca tacgagtcgt cttttctgta tctaagaata
    30721 acgaagaggc acccccaacg acaattaagc gtgtatcggt tccttcaagc atctcaatca
    30781 aatgggacaa agaagtttga tgcatttctt cttttccttt gggagcgttg aaagcatcaa
    30841 cgacaacatc aaaagatgca agatcagtag ttgtcaactc aaataaatcc ttttgtataa
    30901 acggaacatc tgatggcaat ttttctggat gacgcacgat tgccgtcacg tctaaatctc
    30961 tggacaatgc ttcgtctaaa ataaatgaac ctgcatggcc agtagctcca atgagtgcaa
    31021 ttttcataaa aaacctcctt ttttattcct gtccattgta agcagttgct gaatattcgt
    31081 caaagaatcc gatttttgaa aagctcagtg cgcattcctt tcatggttcg tgatcattgg
    31141 gtatagttaa attaagaaaa ctgattgata aaaatagaag ttgaaggaga taagaaatga
    31201 aagagtggtt agatcgtgcc tttttaccaa tgataacatc tgttgtgaat cttagaccct
    31261 taactgcttt gcgtaatgga atgacttaca cgatcccaat aattattgtt ggctcgctgt
    31321 ttttcttatt tgctcatttt cctgtggatt acatcgctca gtggatagac aaaacaggat
    31381 tttctatgta ttttacacaa gtataccagg ctacatttga tgtgatagcc ttttggatcg
    31441 tttttgccat tgcatacgct tatgcaaaag aagaagacat cgatcgctta ccagtcggca
    31501 taacagcctt gtctgcttat ctactgctta ttcagccact aaatactcag atagatactg
    31561 tctggacagg tgctgaaggg atactaggag cagttacaac tgggctcatc atcggatggg
    31621 gctatgcacg tttagtaaga cttgcgaaag ggtggaagca aagagagaag atacctgaaa
    31681 atgtttggca gtcttttact tcattgattc caatcattgt tatagtcact tttacgctta
    31741 tcggttcagc ggtttttcaa cagttgttca gaatgactgt tgttcaagga gtatggacat
    31801 atgtccagct tcccattcaa tcgttcatag acacgttgtt tggtgtgctt ttattaggtt
    31861 tttttgttcc gtttttatgg ttatttggtc ttcatggatc tgcgatggtc aatgggttgg
    31921 ttagtcccgt cttgcaagcg aattcattag cgaatgcaga aatcttagcc tcaggacaag
    31981 agttgacggt ggcaaatggc ggacatatcg ttactcagca atttctcgat cagttcatga
    32041 cggttacggg tgctggattg acgttagggg ctgtattttt tatgacggtt tttgcgaaat
    32101 cgaagaaata cagagaatta ggaaatcttg ctcttttacc agcctttttc aacatcaatg
    32161 aatcaattat tttttccaca ccgatcgtga tgaacccgat gatggctgtt ccttttattt
    32221 tcgcaccggt cgtttcagga ttgattacct attccgcttt atattttgga ttcgttcctt
    32281 tatttacagc agtccaagtc ccttggacaa caccgccgat cctctcgggt ttcttagtag
    32341 gcggagtgcc tgcagcgatt ttacaaggta tcgtgttgtc aattagtttt ttcatttatt
    32401 tcccattttt gaaaaaaata gattctgcaa actgcaaaaa ggaaagacag acaaaaaatg
    32461 atggagcagc agaaaagatg aacgactaaa aaacaaaaat actcctgtcg ctttagattg
    32521 ggaaaatcac caatctagga tgacaggagt atttatagtc atgtttatac tagccctttt
    32581 tggacagcta attgaacaag aacaactaat gtgttcattc ctacgtgtgt gatcatcgat
    32641 gtccagatcc gaccagttgc tttgtactct aggctgaaga agaaacctag gaaaaagtag
    32701 attagcagat gaccatcatt atgagcaaac gcaaacagca aagagctgat cactgctccg
    32761 acccaaaaat ttgaatatcg actgatgatc cccagtatac tgcggcggaa tacgaactct
    32821 tccatgatgg gtccagcaac agttgtcgcc aaaataaaag cgggtgcttc catgatcatc
    32881 tggatgatat tttgtgtatt ctcggaaggg gttgctttgc caaacaatac agattctaac
    32941 atgacagcga tgctttgcag aatcaatgcg acaaagattc caaccaatcc ataaataaca
    33001 atctttggag cagaagcttt ttttaccgct gattcgatcg ctaagggttc acgttgctta
    33061 aaataaaaaa tagctaggac aacggcgcct agtatataca tgactgcagt tgctgtgatc
    33121 aattcagacg tagattttac aagtcctatc gaagctagga tcaagggtga gataaaaatt
    33181 agaccatagc aaaaaatgct gaggaagcta tattttttta aagacatgta attcctcctt
    33241 ttaagggata acaagcatgc aaggcaagct tatatcgatt gatttcaatt atatcataat
    33301 ttcaataagc agctgtcaga ataaaatttc ttttctttga tttaaaagct tgcaaaaaag
    33361 agggagaatg agtattatta aaaatgtagg ttagcactcg aaggttaaga gtgctaacag
    33421 attaaaaaaa gagttatcgg agggatttat cgtgttaaaa ccattaggtg atcgcgtcat
    33481 tatcgaagtc gcaaaagaag aagaaaaaac tgtcggagga atcgttttag cttctgctgc
    33541 taaagagaaa ccccaaactg ggacagttgt tgcagtcggt gaaggccgtt tgttagaaaa
    33601 cggtgaaaaa gttcctgccg ctgtaaaagc aggcgaccaa gttatgttcg aaaaatacgc
    33661 cggtacagaa gtaaaatacg aaggaaaaga atatctgatc gtcgcaggaa aagatatcat
    33721 ggcgattgtt gagtaaattt ggaagataaa aaaataatca aaaataatca atgaggtgaa
    33781 tcaatcatgg caaaagaatt gaaatttgca gaagacgcac gcgcagctat gttacgcggg
    33841 gtagataaat tagcagatac agtgaaagta acattaggac ctaaaggccg aaatgtcgta
    33901 ctagaaaaat cttacggctc tccattgatc acaaatgatg gtgtgacgat cgcaaaagaa
    33961 atcgaattag aagatcattt tgaaaacatg ggtgcaaaac ttgtatcaga agtagcatca
    34021 aaaacaaatg atattgcagg agacggtaca acgacagcaa ctgttctgac acaagcaatc
    34081 gttcgcgaag gtttaaaaaa cgtaactgca ggtgctaatc ctctaggcat tcgtcgcggg
    34141 atcgaattgg caacaaaagc agcagtcgaa gaattgcata atatctcaac tgttgttgat
    34201 tcaaaagaag ccattgcaca agtagcagct gtttcttcgg gttctgataa agttggacat
    34261 ttgattgctg atgcaatgga aaaagttgga aacgacggtg tcatcacaat cgaagaatca
    34321 aaagggatcg aaacagaact agatgttgta gaaggtatgc agtttgatcg tggttactta
    34381 tcacaatata tggtaacaga taacgacaaa atggaagctg ttctagaaaa cccatatatc
    34441 ttgatcacag acaaaaaaat ctctaacatc caagatattc tgccattgtt agaacaaatc
    34501 ttgcaacaat cacgtccatt gttgattatt gctgacgatg tagatggtga agcattgcca
    34561 actcttgtat tgaacaaaat ccgtggaaca ttcaacgtag tagctgtaaa agctcctggt
    34621 ttcggcgatc gtcgtaaagc aatgcttgaa gatatcgcta ttttgacagg tggtacagta
    34681 attactgacg atctaggttt agaattgaaa gatgtaacaa tcgaaaactt aggaaatgct
    34741 tctaaagttg ttgtagataa agacaataca acgatcgtag aaggttctgg agaaaaagaa
    34801 gcaatcgaag ctcgtgtgca attgatcaaa aaccaaattg ctgaaacaac ttctgatttt
    34861 gaccgcgaaa aattacaaga acgcttagct aaattagcag gtggtgtagc tgttgttaaa
    34921 gttggtgcag ctactgaaac agaattgaaa gaattaaaat tacgcattga agatgcattg
    34981 aatgctacac gtgccgcagt agaagaagga atggtctcag gtggtggtac agctctagtg
    35041 aacgttatca gcaaagtttc tgctgtagaa gcagaaggag acgtggcaac aggtatcaag
    35101 atcgttgttc gtgctttaga agaaccaatt cgtcaaatcg ctgaaaacgc tggttatgaa
    35161 ggatcagtga ttgttgataa attgaagaac gtggaactag gcactggttt caatgctgct
    35221 actggagaat gggtaaacat ggttgaagca ggtatcgtag acccaactaa agtaacacgt
    35281 tctgccttgc aaaatgccgc ttccgtttct gccttgttat taacgacaga agcagtcgtt
    35341 gcagataaac cagaaccagc agcgccagca gctcctgcaa tggatccatc aatgatgggc
    35401 ggtatgatgt aagaaaaagc aacttgctta tctacaactg atcattagtg aaagacagat
    35461 aaaaagaggc tggaacagaa gcgtttaact ccaagaaata agaagaaatt cacggaaatt
    35521 gcttttcaaa tttttgtgaa tttcagctta tttccgaagg agttgcttct gcttccgctg
    35581 tttatacgtg tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctcttttt
    35641 tgtgtgtaat tataatgaaa atagttattt ttaatagtta taatatttta tctattaaaa
    35701 gtaaatatcc attc
//
LOCUS       DAFEHS010000019        35584 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFEHS010000019
VERSION     DAFEHS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.019"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            266..2032
                     /locus_tag="LP327_001044"
     CDS             266..2032
                     /locus_tag="LP327_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBL1725177.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2134..3393
                     /locus_tag="LP327_001045"
     CDS             2134..3393
                     /locus_tag="LP327_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL1725178.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3540..4841
                     /locus_tag="LP327_001046"
     CDS             3540..4841
                     /locus_tag="LP327_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1725179.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4844..5695
                     /locus_tag="LP327_001047"
     CDS             4844..5695
                     /locus_tag="LP327_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1725180.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5708..6523
                     /locus_tag="LP327_001048"
     CDS             5708..6523
                     /locus_tag="LP327_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBL1725181.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6674..7840
                     /locus_tag="LP327_001049"
     CDS             6674..7840
                     /locus_tag="LP327_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL1725182.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8070..8717
                     /locus_tag="LP327_001050"
     CDS             8070..8717
                     /locus_tag="LP327_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL1725183.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8801..9745
                     /locus_tag="LP327_001051"
     CDS             8801..9745
                     /locus_tag="LP327_001051"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBL1725184.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9880..11331
                     /locus_tag="LP327_001052"
     CDS             9880..11331
                     /locus_tag="LP327_001052"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBL1725185.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11352..12188
                     /locus_tag="LP327_001053"
     CDS             11352..12188
                     /locus_tag="LP327_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL1725186.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12188..12847
                     /locus_tag="LP327_001054"
     CDS             12188..12847
                     /locus_tag="LP327_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL1725187.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12973..13662
                     /locus_tag="LP327_001055"
     CDS             12973..13662
                     /locus_tag="LP327_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725188.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13668..14624
                     /locus_tag="LP327_001056"
     CDS             13668..14624
                     /locus_tag="LP327_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBL1725189.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14668..15180
                     /locus_tag="LP327_001057"
     CDS             14668..15180
                     /locus_tag="LP327_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1725190.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15436..15984)
                     /locus_tag="LP327_001058"
     CDS             complement(15436..15984)
                     /locus_tag="LP327_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL1725191.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16073..17254)
                     /locus_tag="LP327_001059"
     CDS             complement(16073..17254)
                     /locus_tag="LP327_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBL1725192.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17490..18998)
                     /locus_tag="LP327_001060"
     CDS             complement(17490..18998)
                     /locus_tag="LP327_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBL1725193.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19157..19348
                     /locus_tag="LP327_001061"
     CDS             19157..19348
                     /locus_tag="LP327_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725194.1"
                     /translation="MKKRQKKENAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19551..20468
                     /locus_tag="LP327_001062"
     CDS             19551..20468
                     /locus_tag="LP327_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBL1725195.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20461..21534
                     /locus_tag="LP327_001063"
     CDS             20461..21534
                     /locus_tag="LP327_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725196.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21682..22200
                     /locus_tag="LP327_001064"
     CDS             21682..22200
                     /locus_tag="LP327_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725197.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22181..22573
                     /locus_tag="LP327_001065"
     CDS             22181..22573
                     /locus_tag="LP327_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBL1725198.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22649..23584
                     /locus_tag="LP327_001066"
     CDS             22649..23584
                     /locus_tag="LP327_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBL1725199.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23717..24496
                     /locus_tag="LP327_001067"
     CDS             23717..24496
                     /locus_tag="LP327_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL1725200.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24489..24806
                     /locus_tag="LP327_001068"
     CDS             24489..24806
                     /locus_tag="LP327_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL1725201.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25050..26660
                     /locus_tag="LP327_001069"
     CDS             25050..26660
                     /locus_tag="LP327_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL1725202.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26817..27665
                     /locus_tag="LP327_001070"
     CDS             26817..27665
                     /locus_tag="LP327_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL1725203.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27896..28375
                     /locus_tag="LP327_001071"
     CDS             27896..28375
                     /locus_tag="LP327_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725204.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28408..30636
                     /locus_tag="LP327_001072"
     CDS             28408..30636
                     /locus_tag="LP327_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL1725205.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30765..32408
                     /locus_tag="LP327_001073"
     CDS             30765..32408
                     /locus_tag="LP327_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL1725206.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32481..33026)
                     /locus_tag="LP327_001074"
     CDS             complement(32481..33026)
                     /locus_tag="LP327_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBL1725207.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33049..33534)
                     /locus_tag="LP327_001075"
     CDS             complement(33049..33534)
                     /locus_tag="LP327_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL1725208.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33524..34336)
                     /locus_tag="LP327_001076"
     CDS             complement(33524..34336)
                     /locus_tag="LP327_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBL1725209.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34475..>35584)
                     /locus_tag="LP327_001077"
     CDS             complement(34475..>35584)
                     /locus_tag="LP327_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL1725210.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11645 a         6401 c         7114 g        10424 t
ORIGIN      
        1 aatatacttc tacgccaaaa actgttaaaa aacttttact ttagaaaatt ggagaaattg
       61 atcgcttaag ataaattcag gacactaaat agtacaggaa gtagactaaa acttttaaaa
      121 gttttaagtc tatctatttg gcaagtttaa aaaataaaaa ttcggtatca aaaaactgtt
      181 accgataaca tttttgaaag cgttgtcttt tgcttctcta tcacttatag tgatgacgtc
      241 atattaaaac acaaggagga aatcaatgga tacagctgca atttatcatc gaccggaaag
      301 tgaatatgca tttctatata cgaatacaag atttcgtatt cggcttcgta ctaggaaaaa
      361 tgatatcaaa aaagtatatg tgctatgtgg agatccatat accataacaa ctgaaaaatg
      421 gtatcaaaaa caacgaccaa tgaaaaaatg tctaagtacc aatgttcatg attattggga
      481 aattgaagtg acgagcgaaa caagtcgctt acagtatgcg ttccatgttg ttggggaaga
      541 taacacggac tgtttttacg gtgatcaagg aatttttcct tatcgagaaa atgtgattac
      601 tgaaccaaat ttttattttc gaatacctta ttttcatcaa atcgatcgat tcactattcc
      661 agaatgggta aaagaaacag tatggtatca aatttttccc gaaagatttg caaacgggga
      721 caaagcaaat gaccccgaac acactttacc ttggggaagt aaagatccag accgagaaga
      781 ctttttcggg ggagatttgc aaggtgtgat ggatcatttg gattacttga cggaccttgg
      841 catcaatggc atctattttt gtcccatatt taaagccaca tccaatcata aatacgatac
      901 gattgattat tatgaggtgg atcccgcttt tggcgataaa aaacttttaa aaaacttgat
      961 tgatgaagca cataaaagag ggatacgtat catgttcgat gctgtattca atcatatggg
     1021 tgtccattca ccacagtggc aagacgttct caaaaacgga gaaaaatcga tctataaaga
     1081 ctggttccac atccgttttt tcccagtaga ttcttatcag atgactgact taccagaaac
     1141 tgctgaaaat atcccatatg atacttttgc ttttactcca ttcatgccga aactaaatac
     1201 agcaaatcca gaagtgcaaa aatatttgct ggatatcgca acttattgga taaaagaatt
     1261 cgatattgat ggatggcgtc ttgatgttgc taatgaagtc gatcatcatt tttggaaaaa
     1321 attcagagaa gcagttacag aaatcaaacc agacatctat atccttggag agatttggca
     1381 ttcctctcaa gcttggctgc aaggggatga attccatgca gtgatgaatt atgcatttac
     1441 agattcgatt aaagattatt ttgcaaaaaa gaaaatcaca gcttcacaaa tggtcagtgg
     1501 aatgaatcat cagcaaatgc tttacagaga tcaggtgaac gaaggaacat tcaacttatt
     1561 agattctcat gatactgcca ggatcttgac cctttgccaa ggtaacaaag aattgatgaa
     1621 gtctgtgatg gcctttatgt ttcttcaaaa agggtctcct tgtatctatt acgggacaga
     1681 aatagggatg actggtgaag atgacccgga ttgccgcaaa tgtatgatat gggagaagga
     1741 ggaacaagat ctagagctat tcggttttat aaaagaactt gtttctctaa ggaaacagtt
     1801 gtctaagatt atttctgaag gttctactca gtggctaata gtaaatgatc gtgaagataa
     1861 gctctatttc acacggaaac tagagggaca gatcatctat gtttacttta atcagtcaaa
     1921 agaaccttgg gtagtagaac aggaaaatga ggtgattctt agtcaaaatt gtcagttgtt
     1981 agaagacggg aaagcagaga tacgacaata cggatttttg atcatgtgtt gaaaactgtt
     2041 accggtaaca acaagaaagc gtttgcaaat gaagatttgt gcccattata ataagggtgt
     2101 aacagcacgt tgaatacaaa ggaggaaatg aagatgaaaa aaagaagtgt gaaacagttg
     2161 ggtttagggt taatctcttt aggaatctta ggcggattag cggcttgtgg caatggtaac
     2221 tcttcagcag aaggggaagc cgatgaagga aaaacgttaa cagtttccgt tgattctgga
     2281 tataaagatt atataaacga aataaaaaac actttcgaaa aagaaaacga tgtgaaaatc
     2341 aagctagtag aaaaggacat gttcgatcag ttggaatcat tagcgttaga tgggcctgct
     2401 ggaaagggac ctgacgttat gatggcagca tatgaccgga tcggtgcgtt aggacagcaa
     2461 ggacatctgg cggaagtaaa actgggaaat gaagcagctt acgatgaaac agacaaagcc
     2521 caagtgactt atgatggaaa aatctacgga gaacctgcag taatcgagac ccttgttttg
     2581 tactataaca aagatttagt cgatacagca ccatcaacgt ttaaagattt ggagaatcta
     2641 tccaaagaca gtcgttttgc ttttgagtca gaagcaggaa aaaatacagg atttttagct
     2701 aaatggactg acttttatta ttcctatggt ttaattgcag gttacggtgg ctatgtattt
     2761 ggtgatgatg gcacaaatcc ttcagatatc ggtttgaata atgcaggtgc tgtagaagga
     2821 atctcttatg caacagattg gttcaaaaat gtttggccaa agggaatgca agacataaaa
     2881 agtgcaggag acttcgccag ccagcaattc atgtccaata aaactgcagc aatcattgac
     2941 ggtccatggc aagcgcaaac atacaaagaa aacaatatca attatggtgt agccaaaatt
     3001 cctactctaa ataatggaca gccttatcaa ccgtttggcg gaggaaaagg atgggtagtc
     3061 agcaattatg ctaaaaacaa agacctctct caaaaatggc ttgattatgt aacgaatcag
     3121 gaaaatcaag aaaaattctt tgaaatggta aatgaaatcc ctgcaaacca acaagctagg
     3181 gaaacagcaa aggctaaaaa cgatgaatta acaacagctg tgattgaaca atatgaaaca
     3241 gctcaagcga tgccaaacat cccagaaatg ggcgaagttt ggacaggtgc agaaaacttg
     3301 atgttcgatg cagcatcagg ttcaaaaaca ccaaaagaat ccgcagatga agcagtaaaa
     3361 acaatcagtg aagccataga acaaaaatac tgattaaaat gataaccgtc catttttata
     3421 aaaaatggaa caggtgcttc agataaaagt ggcaagagcg agatggaaaa ttatcttgct
     3481 cttgttgcca ttatcttaaa agcagtttgc ggtttagcaa gtttagaggg ggcattcaga
     3541 tgcagcaaga taaaacaagc aatatcaacg aaaaaaaaat cacgctttgt tcacttattc
     3601 cagggatggg gcagtttgta aatggccaag cttttaaagg tatcgttttt ttcgccatat
     3661 tcgtcctatt cattggtcag atgcttctgg gcggatttca tgcattggta ggattggtta
     3721 ctttgggaag tataccaatg gaagatcatt cattatttat tttgattgaa ggcactttgc
     3781 aattcatcat tacaatcatt ttcttgattt tttacggttt gaatctttta gacgcttatc
     3841 gagtagcgaa gatgcgtcaa gagggcaaaa aaatcaatcg gacgatgaag gaagtcatac
     3901 ataatatagg tgatgaaggt tttccttatc tattgacact tcccgcttat cttctcatga
     3961 tttttacgat cattttccca gtcctagtta cattgttcac tgcatttact aattatgact
     4021 tcaatcatat tccaccagcc agtttaattg attgggtagg ggctaaaaac ttttttagta
     4081 tcttcttttt aagctcatat cgtaatactt tttttgcagt cttttcgtgg accgtgattt
     4141 ggactttatg tgcaacaact ttgcaaatca cccttggtat tctcacagcg gttgttgctc
     4201 accagaaatt tattaaattc aaacgtattt tcggtatcat tttcttatta ccatgggcgg
     4261 tcccagcttt tattactatc atgagttttt cgaatatatt caacgatagc gtaggtgcca
     4321 tcaatactca agtgattcct ttcataaacc agcttccttt tgtagatatc agtgcaatct
     4381 cttggaaaac ggacccgaac tggacgaaag tagcaattat cttagtgcag ggctggttag
     4441 gatttcccta tatctatgtg atggttacag gaatcttaca atcgatttca gaagacttat
     4501 atgaagcagc aaaaatcgac ggagcaaatg catggcagcg cttttcaaac atcacgcttc
     4561 ctgctgtctt cctagtggca gcgccaacat ttattacaca gtatacgggg aatttcaata
     4621 atttctccat gatctatctg tttaacaatg gcggtcccgg aagcgtaggc ggaggagctg
     4681 gttctactga tattcttatt tcctggatct ataaacttac gactggaact tctccgcagt
     4741 attctatggc ttcagcaatc acgttgatta tctccgtcat cgttatctgt gtgtcattgc
     4801 tggtatttaa aaagacaaat gcgttcaata tgggggatta acaatgaaaa aatttcattc
     4861 gagtgctcga ttccaaaaac gactttctca cttttttact tacttttttc ttttaatact
     4921 gtcagtcatt atcatctatc cgcttttgat cacagcaagt actgccttca agtcaggaaa
     4981 tattgcagct tttagtttag atctttcaag tgactggact ttaggaaact ttactcggct
     5041 attcagtgaa acactttacg ggacttggta taaaaataca ttgatcattt ctgtcctaac
     5101 aatgatcgta caagtgactg tagtaacttt ggcgggctat acttatagtc gctatcgttt
     5161 tatcggccgc aaatcaagtt tgatgttctt tttagtgatt cagatggtac cgactatggc
     5221 agcattgact gccttttatg tcatggcttt cttattagaa gcgctcgata agtactggtt
     5281 cttgacaatg atctatatag gcggtggaat tccaatgaat acttggctga tgaaaggata
     5341 ctttgacact gtaccaatag atttagatga atctgctaaa cttgatgggg cagggcattt
     5401 caggattttt ttccagattg tattaccgct tgtcaaaccg atgctcgccg ttcaagcatt
     5461 atgggctttt atgggacctt ttggggattt catgttagct aagttcctgc ttcgaacacc
     5521 tgaaaatcag actgttgctg tcggcttaca aacatttatt tctgatgtga caaaccaaag
     5581 agtctctctt tttgctgctg gggcaatttt gattgcacta ccgatttgtc tattattctt
     5641 ctttttacaa aagaactttg ttaccggcct gacatctggc ggaacaaaag gataggagca
     5701 gagtatcatg caaacatttc cgttaaacta tttttccgca ttacgtacgc caactcaagt
     5761 gtttgcggga cgcaaactat tatcatggcc taaatttttc ttgatatttg tatttttagt
     5821 ttccttgatg gtcatgcctg tgactctttt ttatgccaat cagatccaag cgattccatt
     5881 agaacaattt ctatccgttc attcgctgat cgatgaacaa ggaacccaaa aattttctga
     5941 acttgaatta tctgaaactg ggttacaagc atcgcaacaa acgatagctg tgacccctga
     6001 aattttggta ggtgtgtctt tatctgagaa acaacaatcc gatcatggaa cttttattga
     6061 ttttgagaag gaacaatggg tgatccaaca aaaggataaa tctggtatac gccgctatac
     6121 gatgaattat agcccatctt tccaaccaga ttctgtacgt acgcccgaag atttccaacg
     6181 atttttagaa agagaatttt atgccagtaa tcgtccaaca atcattttat cttatagttt
     6241 aagtcttggt cttgtcctat ttgtcatgac gagtctcatt ttattcgggg cttccttttt
     6301 cttgtggatg acaagaaaaa gccaactttc atccattcat acattcaaag agagtgcaaa
     6361 tttgatgctg aatgttatgg gaataggttc catggcagca gcagttgtag ggtttatcca
     6421 ctttgacttt atcttgatgc taagtgtcca aacaacgata actgtcttgc tgcttttatg
     6481 ggtttttgtc aaaacaagat tcaaagataa gcgtgtcgaa tgacaagttt aagcgattta
     6541 tgcgacaatg aatgtgaaaa aaatgaaaaa taaagtagtt tttgaaaaat ttctgaaaaa
     6601 attaaacttc ttagttgatt cttttctttt ttagtattaa cgtaataggg tagttttttt
     6661 aaggaggaaa atcatgttag agaaaacttt atacaatcaa ttattttcac gttctttctc
     6721 gctccctgtc gaagtaaatt attgggacgg tacaacgaaa caatacggag atacggacac
     6781 tcctccccaa atcaaaatca atatccatga agaaatccca atgaaagaga ttacaagtaa
     6841 cgcatctttg gcactaggag aagcctatat ggatcatcga atcgagatcg agggaagtat
     6901 ccaagcattg atcaacgatg cttatcagca agcaaacagc tttttacgag gaaaagatta
     6961 tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca
     7021 tgcgcattat gacttaggta acgactttta taaactttgg ctagatccaa ctttgactta
     7081 ttcttgtgct tatttcaata cacctgaaga tacgttggaa caagcacaaa tcaataaagt
     7141 ccatcatatc ttagacaaac tctttatcaa agaaggcgaa acgttgcttg atatcggttg
     7201 tggctgggga acattgatgt tcactgctac caaagagtat aatgtcaagg caactgggat
     7261 cacactaagt gaagaacaat acgatttgat taccaaaaga ataaaagaag aacatttaga
     7321 agataaatgc cgtgtattat taatggatta ccgcgaacta aaaggcgaaa cgtttgatca
     7381 tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag gttatttcaa
     7441 agtggtaaaa gatttattga aaccaaaagg aacagctttg atccatggaa tcagccgaca
     7501 acaaggcggt gcgacaaatg cttggatcaa taaatatatt ttccctggcg gttatgtccc
     7561 tggtgttact gaattagttg ataatattac aaaaaacgat ttgcaactga ttgatttgga
     7621 aagcttacgt cgtgactacc aattgacatt agaacattgg acaaagaatt ttcatcaagt
     7681 ccaagatcaa gtcacgaaag aaatgggtga ttccttctat cgaatgtggg atctatattt
     7741 acaagcttgt gcagcttcat ttgaatcaag taatatcgat gtgattcaat atttgcttgt
     7801 tcaacctgga aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt
     7861 aaagactatt taaaaagaag ctaagtttta tgatgctcca gaagtgaact tttttgtcta
     7921 acttctagga ttgtctctaa agcttagttt ctttttcttt atattttcat aataaaagtt
     7981 tggttcttta atttttaaac atgacagaaa attttttctt cgttatactt gtaatagaat
     8041 actattttat aacaaagggg atcgaatcaa tgaaaaaaac tgttatttat actagtttat
     8101 ttgcagcttt gtttttagct gcttgtcaat caaataccaa tcaaggcaca tctgggaata
     8161 cgtcttccag tagcgaacag cgctcttcac aggtatcgga ctcctcaaca aaagcaagca
     8221 catcttcgtc tagccagaca aaaagttcta gtgaagcaag tactcagtca gatatcaaag
     8281 tgtctgcagc tcgtgcgatc gaattgttcc aagataaata cccagatact gcaattacta
     8341 gtttggaatt agatagtgat tggggatctt acttctacaa gatagaaggt gtcgatgatc
     8401 aaaaagaata cgaagcaaga attgatgctc aaaatgaaaa aatggagaca gagaatccag
     8461 aaactctaga tcgagatgaa caaaatggtc aaaagaaaaa agaagatggt ctggatgttg
     8521 ataaattgat cacgattgag gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta
     8581 cggcaacaga ttgggatttg gataaagatt taggtactac ttattgggaa gtcaaagtaa
     8641 aagacggcaa tcaaagtaca tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa
     8701 ctgaacaaga tgattaagaa tttcttcgga aaacaagagt tgatttttct tttagtcatg
     8761 atacactaga caaaataact tgtttgaagg agcgatttac ttgagtttag aaacgacctt
     8821 tgccaatcat atctttgcca atcctttgat gaatgcatct ggtgtccact gcatgactac
     8881 ccaggaatta gatgaactgg ctcattcaga agcaggagca tttatcacta agagctgtac
     8941 gatcaatgaa cgaaaaggaa atcccgaacc acgctatttt gatgttcctt tagggagtat
     9001 caattctatg ggacttccaa acttaggttt ttcttattat ctagagtatg ctcttgcgta
     9061 tgaaaaagtg caggaaaacc aaaatcaacc gttatttttt tccattgccg ggatgagtgt
     9121 ccaggaaaac ttggaaatgc ttgagaaaat agaaaaaagt gggtttaatg ggatcactga
     9181 attaaattta tcttgtccca atgtgcctgg aaaaccacaa ctagcttacg attttgaggc
     9241 cacttatgaa acactgaaag aagtattcag catcttttct aagccgctag gaatcaaatt
     9301 gcctccttat tttgattttg ctcattttga tcagatggct gatatcttga atcagtttcc
     9361 attgacctat gtcaatgcta tcaacagtgt gggcaatggt ttgtatatcg atacagaaca
     9421 agaagcagta gtcatcaaac caaaagaagg gttcggcggc attggcggag agtatatcaa
     9481 gccgactgct ttggcgaatg ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat
     9541 cattggtaca ggtgggattc ggacaggaca agatgcattt gaacatctgc tatgtggcgc
     9601 aagcatgtta cagattggta ccgagcttca taaagaaggg ccggaaattt tttctagaat
     9661 cataaaagaa ttaacgcaga ttatgtcaga aaaaggatat acgtcaattg acgaatttaa
     9721 aggcaaatta cgcaccattt catagcaaag agacgaaggt catgattgat cttcgtcttt
     9781 ttttataaaa aacactcgtc tgttcattcc tgtgcaagcg tactcattgt atgttagacg
     9841 aacttgtggg aaaataacag aggcaaaaag gaggataata tgaatgatgc aacagaatta
     9901 gcaaaagagc ttaaagaaaa agaatttagt tttcaagaac tagttacgga tatacagaaa
     9961 aaaataaaga agcaaaatca tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt
    10021 aattcccacg agctattgat tccccagtca gaaaaagggc cattggctgg tatcccattt
    10081 cctttaaaaa tgctcggtca agaaaaaaag ggatggctag ctacttcagg atctcgttta
    10141 tttgaaacac accgagcttc tcatacatct aattatgtgc ggcaagcaga agcgataggt
    10201 ctagttcctt ttggacaaac caatgcgcca gagtttggtt tcaaaaatat tacagaccca
    10261 gtcatctatg gcccagcaag aaacccttgg aaccttgaat gtacaccggg cggatcaagt
    10321 ggaggagctg ctgctgcagt ttcatcagga atcgttcctt tagctggtgc tagtgatggc
    10381 ggtggttcta ttagaatacc cgcttcgttt tgcggattga ttgggctgaa accttcgcga
    10441 gggacgatgc cagttggtcc atatgcttgg agaggatggc aaggagctgc tattgatttc
    10501 ggcttgacag tttctatgag agacacggag gctttatttt atgggatgag aagtatccat
    10561 tccggtgcgc catatcaagt atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg
    10621 ttgaagatag ctttttgtgt tgattcaccg atccactcac agattagtga agaggcagta
    10681 cttgctgtaa aaaaagctgt agatttcctt ggaaaagaag ggcatgattt gacagagatt
    10741 ccttacccac tagatggtcg taaactgatc acgtcttatt atcagatgaa tggcgcagaa
    10801 acagcagcaa tgatccgctc gattgaaaca ggcatccagc gagctgtcag aaaagaagaa
    10861 cttgaaccca tttcttggac tttgttccaa tatggtcaga agctctctgc cgctacttac
    10921 atcgattctc tgcatgtatg ggatgaagca gcaattgtca tggaaaacct acatcaagaa
    10981 tacgatctat tcctttcacc aacgactgca tttaccgccc ctaaaatcag cgaagatttg
    11041 caaagtgatg cgataagagc acagatggaa cacatcagtg attgttctga agtagaaggt
    11101 ttagacgttg tttcttccat gtttgaggag agcttgaaga tcacacctta tacacagttg
    11161 gctaatctga ctggacaacc agcaatcagc ttgcctactc atttgacaaa agaaaacatg
    11221 cctctaggaa tccaatttat ggctgccaga ggaagagaag atttgctatt tcaaatcggc
    11281 tatctatttg aagcaaatca tcagttccat ttaccaaatc catggaaata gcagaaacaa
    11341 ggagaagaag aatgaagaaa aaaagattgt ttgctctaat aactagtgcc ttttttattt
    11401 taagcgcttg cggtacagct gccgctaatc aggaagataa cagctggagt aatgtagaaa
    11461 aaaagaaaga attagtagta gcgacttctg gcacactgtt tcccgcatct tattataatg
    11521 atcaaaatga attagttggt tatgatgtag atttggcaaa agaggtggct aaacggttaa
    11581 atgtcaaaat ctcctttaaa gaatacaacg tggatggaca aatcacttct gtttcaaaaa
    11641 gagaatcaga ttttgctgcg aacgattttg gtatcacaaa agaaagagaa aaaaaattct
    11701 ccctttcaga gactttcaaa tattcatttg acagtatgat cgtacgaaaa aaagatgatt
    11761 caggaatcca ctcacttgaa gatcttaaag ggaaaaaagc agctggcgaa cccaatacta
    11821 gctatatgcg tttagcggaa aaacacggag ctaaattagt cacatatgac aatgccacga
    11881 atgatcagta tttaagcgat gtagcaaatg gtcgaacaga tgttattcta aatgattatt
    11941 atctgcagaa aatggccgta tctgctttac cagatatccc tgtaaaaata ctggagaacg
    12001 tttatttcaa tgaaaacgaa agtggttttt tattgaaaga aggaaatgat gctctgaaaa
    12061 agaaaatcga tggcgtatta aaagagatga aagaagatgg gacgacaaag aaaatatctg
    12121 aaaaatattt tcaaactgat gtttcagtca aaccgaaaga aacgatggct gagtcggtga
    12181 gtgagtgatg tactttcctc taattgattt ttccctcatg tggacttcac ttcctttcgt
    12241 actgcaaggc ttggtataca cattagggat tggcttcgtg tcgtttcttt taggaaatct
    12301 ggtagggttg cttttgacag ttttaggtct tttggattgg ctgcctctga atgtatttat
    12361 tcgtttttat ttatcgttct ttagaggcat tcccgcacta gtcctgctgt ttctgttata
    12421 ttttgggtta ccttatcaat taagtgcctt aacggcttca gtcatctgct ttacgattac
    12481 cagcagtgca tttattggtg aaatctatcg aggatcacta gcaggcgttt cttcaggtca
    12541 gtgggacgct ggtttagctt ctggtttttc tttctttggg gtattgaaat atatcgtttt
    12601 gccgcaagct tttcgaatct cagtaccggc actcagtaac gtagctatgg atctagtgaa
    12661 aggtacttcg ttagcagcaa caatcacagt gccagaaata tttcaaaaag caaagatcat
    12721 aggcggtcgg acattcgatt atttttctct ctatatttta gttgcactga tttattgggt
    12781 catttgtatc gggatcgaaa aagtgcaaca gtatttagaa aaacgttcta tgcatcagtc
    12841 aatctgaaaa atagaaatgg tgctagcgtg gctggcacca tttctatttt ttgtttcttg
    12901 tatattctgc ctagtatttt aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg
    12961 gaggtagaaa acatgaagac cccgctaacc atcgaaatag aatcctcgtt ataccaatat
    13021 tgcatcgagc aaggcggctt ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt
    13081 gtagatacgc ttagctgctt gacaaaattt gatggaagcc gagaatggcg atgctatgag
    13141 ctgaaagtga caaaagcaga ttttcgctct acagccaaac tgtcatttgt tggacattat
    13201 aattattttg ttttaccaag taaactatat gaagaagtaa aaactgaaat ccctacagga
    13261 ataggtgttt tagtgtatcg tccgtacacg gttgaagagg agatgcctgc ctctggtaca
    13321 tttctgattg cgaaaaaagc agcaaagaaa gagcttgctg taccggaaga agctttgaca
    13381 aaccgcttca tggcttcttt attcagggaa gtaagaaaag caaagcaaat ggagcgaggc
    13441 gtaaaatatt ttccaacaga tcaattgtac aaagaattga aaagacgttc agaaaatcaa
    13501 gatccatttt ctaacgaacg atattatgaa cggtttttag aggaaatagc tagtcaggca
    13561 atcaatgagt tgcaagaaga agttatcgcg cttcagcatg attatgaata tttgaggcaa
    13621 caacagcaga tccgtcggtt gcctacagag ccattggagt gaaaaatatg tactatcctt
    13681 atttaagagg aaaacaattt gacctattag cattaaaaga agcactgtcg cgaggattgc
    13741 tctccaataa aatccaacca gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg
    13801 tgatcgaatt atttcaaaaa aagcaccatc gaatcgctgt gatccaaaat ccccaagtag
    13861 gacaattcaa attattcgat cagcatgtgc acagttggac agtcaaagaa aatagctctg
    13921 tcgtccctgc acagatctta acgccagaaa atctcagtga agtactggat tcacctccag
    13981 cgtttcttgt tttcgatgga cagcactacc caaaagatac agaggtgtgg aaacagttgg
    14041 caggagttga ttcgaaattt ttgataccag atactagtcg ctttcgcatc tggctgcccg
    14101 aaaacaaaat tgtgatccgt gactcttttc aaacgagaaa gcatgttgaa agttatgcag
    14161 ataaaacaga tgactttttt agtgatgact atcttttttt ccacgcagac ggatatattg
    14221 gattttctga tttcacgatc gaaggtagcc gttatttcga taaagggttc cctagccgag
    14281 cgcttgctat ccatctaacg tatattgatg cttacggcaa tatacgcgtg aaacattttg
    14341 tctctgacag taacgactct gctaaagacc aagcattgaa gtttcttgaa gcaggagata
    14401 aaatgaaaaa atggatcatg cgtcaccatc atcaactctt gattacttca ggaatgattg
    14461 aattattggc tctttaccag caacaaaaat tccctggact gggagtgttg aaaaagtggt
    14521 cacttatgca tcatttggaa cttattagtc acctgctaga tcatccaaaa gattggctta
    14581 aatattattc aaaagttcag gaactatatg aagtgatcca atgattagat aatgtaaatg
    14641 aaaaaggaat agaaaagagg aaactggatg tatattcggc aaacaaggaa agaagaagta
    14701 gaagaagtgg tagaaatcat taatgccgca gcggatttca ttgcttcgca aggctctcct
    14761 caatggcaaa acgggcagaa accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa
    14821 gaaagctatg tattaatagt ggaagatcaa attgctggaa cagctgcatt agtctcaagc
    14881 gtggatcccg tttacacaaa aatagatggt agttggaaag aatcaactgg tccttattta
    14941 tcgattcatc gtgtcgcttt agcagaaagt actcgaggaa aaaaactagc aaaaccgttc
    15001 ttagaacatt tgttgacgat tgcagccagc aaaaatatcc aagatatccg tatcgatact
    15061 tatccagcta atgaaccgat ggaaaagacc atctattctg ctggattcac ttatcaaggg
    15121 atgattgaat ttccttttga acacggcgaa agaaaagcat atcaaataat catcaattga
    15181 ctataaaatc atctgagcaa agataaagac attaacacta ctgaaaaaaa caatcatatg
    15241 atagccaaga ggttgtaaca gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa
    15301 atgcttctca aatttttgtg gaattttagc gtattttacg aaatagctgc tttctgaaac
    15361 tcccttcatc gagaggtcgt gacatagctt ttgtcacgac ctctcggctt atcctacgag
    15421 tattattcga tacatttata tcaataatcc tttttttatt ttatctgcag cttcttggaa
    15481 cgctccatcg tttgacacga ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc
    15541 taaatcttct acataaaccg ccagtctggc tgaaatctgt tcagatgaat ctccacgttt
    15601 tttcaggcgc gattgcaatg tttctttaga gatcgtaata aagataggac ggactaccgt
    15661 cccgaaatac tcatataaat gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc
    15721 tactgtcagt ttctttttaa tttctgcttt actggaacca tatttatgtt gagcatattg
    15781 gtcccactct gcaaactcat ttcccttgat catctgattg aatgtattgt cagaaacaaa
    15841 ataataatcc tcttgatctt tttccccagg tcttggtgat cgattcgtgt aagtaatgat
    15901 cttttgttcc ggctgaaaaa tagcttctgc gagtgaagtt tttccagaac cagaaggacc
    15961 gataaataca tagcaataat tcattgtcat ttctcctttg atgattgaac aagtgaaaaa
    16021 gctttggaaa atatttccaa agcttttaaa agtaactttg cttttttgta tattatacca
    16081 gcaagccagt cacaatagca cttaaaaagc tgactaaagt cgctgttagc aaaattttca
    16141 atccgtgttt tgcgacaaga tttccttttt cttcatttaa tcccttcatc gcaccagaaa
    16201 tgatcccgat agaagagaag ttggcaaatg aaacgaggaa gacagatata attgctgttg
    16261 tacgctcagt aaaatggaaa ttaccatttg ctaattctgt catagcaaca aattcatttg
    16321 taaccagttt agtcgccatg atactaccgg catccactgc ttctttccat gggattccgc
    16381 tgatgaaggc aagaggggca aagatgtaac ctaggatctc ttggaaagtg atgccgaata
    16441 tcccgtggaa gatagcattg atcatggcaa tcaaagcaac aaaaccgata agcatagcag
    16501 caaccgtgat cgccacatgg aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg
    16561 tctgttcttt gttttcttca atgatcagct catcttcttt ttcttctaat acatagggat
    16621 ttacaattga agccagaata taaccgccaa ataagttcaa aacaagggca gtgatcacat
    16681 atttagaatc gatcagtgcc atataagatc cgacgatcga catagaaact gtagacatag
    16741 ccgatacact catggtgaac aaacgtttct cagataggaa gggtaattcc ttttttatgg
    16801 aaataaaaac ttccgattgt cctaaaatag cagaagctac tccattataa gattccaatt
    16861 tgcccatccc gttgatttta cttaatccta gaccaacagc tttcataaat aatggaagaa
    16921 tcttgatata gcgtagaata ccgataatag cagaaataaa tacaattggc atcaatacgg
    16981 aaagaaagaa aggtgcactt cctttatttg ctacaccggc aaaaacaaag tcaacacctt
    17041 gcccagcaaa agcaagaaga tgatcaaaca tttttgccaa tacagaaaca accgctgtac
    17101 caaaagttgt acgtaataat acgaaaccaa gaacaagctg taaaacgaac atcacaatta
    17161 gtggtttcaa tttgattgct tttttgttac tgctgatcaa aaaagcaagc cctaatgtga
    17221 caaataaacc aataatacct aataaataac tcaaataatc actcctattc caaattccgt
    17281 tttattttac ctgttttgca aataaaatac taatctttag tgaaattttc attacttttt
    17341 tagagtgaaa cgcattcatt ttccattaga aaataaagcg aacaaaataa aaggtttaaa
    17401 aagtgtaacg tcttagtaaa gtctataaaa aaacgaaact atcgattaag ctaatagttt
    17461 cgttttcaac atttataagt aaactttttc tattggtaat atagattttc ggaagggtag
    17521 acaggtgcgc aggtaatatc gattcctagt tttttcaatg tttgttcatc atttttattg
    17581 accatcacag tagagtgggc ttgtgctccc tcaagttcag ctagtttatc ataagcaact
    17641 tgagcgattg gattcgttac tgcactgatt gataaagcga tcaagacttc attgatgctt
    17701 agtgcatcca aagaggaatg aagtgtcttt gatttcaatc cttgtattgt ttcaagaact
    17761 aatggtgaca ataaaggaat gtcatctgcg atatgggcta aatgttttac tgcatttaag
    17821 ataactgccg ctgacgcatc catcaatgaa cttgtacgcc cggtaatgat gacttcatct
    17881 ggtaattcga ttgcaatcac agcagaagga tcttttgtag gactttgcgc ttgaatctta
    17941 tctgcatagt catgggcagg gactaccacg tttcggtctt caggacgtaa accgacttct
    18001 tccataatca gtttgattcg attaactgct tcttcgtcga tcaatccttt tttgaagtcg
    18061 cattctgttt ggaaataacg acggatgatt tcttgtttag aggcttcttg gacaacggta
    18121 tcatccacga tcccaaatcc tacacgattc acacccatgt cagtaggaga ttgataaaca
    18181 gattctttac cagtaatttt ttcaataata cgtttgatta caggaaatgt ctcaacatcc
    18241 cgattatagt tcacagctac tttattgtag gcatcaaagt ggaaggaatc gatcatattg
    18301 acatctttta gatcgactgt ggctgcttca taagcaatgt tcagcggatg tttcaacgga
    18361 acattccata ctgggaaagt ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt
    18421 tcatgataca gttggttcaa ggaagtagcg agctttccac ttcctgggcc aggagctgtc
    18481 actacaacga ttggtttagt tgtttcaata tactcatttt ttccaaaacc atcttctgag
    18541 acgatttttt ctacgttgac tggatagtct tcgatttctg agtgtttata gaccttgatg
    18601 tttcgtcttt ctagtttatt gataaagact ttcgtggcag gctgtccact atagcgagtg
    18661 ataacgacac tgttgattaa taagccataa ccacgtaatt catcgattaa ccgcaagata
    18721 tccatatcat aagtaatccc ataatcacca cggatcttat tacgttcaat atctccagca
    18781 tagacgcaaa tcagtatttc agcttgatct ttcaattttt gcagcaattt gattttcgca
    18841 tcttcttcaa atcctggaag tacacgtttc gcatgcttgt cgtcaactag cttcccacca
    18901 aattctaaat ataatttgtc atagtggttc actcgttcca aaatatacgc tgactgctct
    18961 tcaatatatt tttctgaatc aaaaccaatt tttttcacta ggttccctcc ttaagtacta
    19021 aagcattttt tcattttttt gaggattcgt caactggaag agcgaaattc ctcaaaaaaa
    19081 caaagaaaat ggtttaaacc aacttgtcta gatagttatt cattggtatg atagtttttg
    19141 ataaggaagg tgctacatga aaaaaagaca gaaaaaggaa aacgcatata aacactatat
    19201 ccgttcgatt tttaccggtt atgaaagaat gcttgaagat cccgaattgg aacaattgac
    19261 gtttacttat ctaaacgaag aaacgcagct aactcgtgat gagcatcagc gcattcattt
    19321 cacaacaaga gatctgccta gtaaataaaa aataagataa aggtcatcgg attcgattct
    19381 gtcctatcag atggaaagac tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag
    19441 cctgttttat ggagcattgc ccgctctttt ttataacaat tttttacaga aaatggtact
    19501 attgaagtat taataagtga aaaacagatg agtaagagga ggcaagtagt atgaaagagc
    19561 cagatacaaa tgcaaattat gagccagacg aactagagga gacgaatgcg gatcaggata
    19621 caggacaaga atcgtcttct ttttacgata tcaaagaaaa agcaagcatg aatctttccc
    19681 aattattaga agacttacag gcttttgaaa aagctgtccg cgaagaagat atccctgaga
    19741 tctaccgttt atacaatggc cgattgaatg aagaattaaa aagaagctca aatgaaaatc
    19801 acgagatcga tcaattgctg atgcgtaaga tccatgatcg ttttttacag gaatttcctt
    19861 ttatggaaca agtagaaaac atctcgccaa cgatgagcta ttacaaaatc ggcacttatt
    19921 accatgatcg gcccacggtc ggtattgatg caagtctgcc agaaattttt gttttgccta
    19981 agatcgatga agagtgggag aaatattccc aaccggagtc aacggactat gacaaaaaaa
    20041 tcaatgagct agacgcaaag gtgatcactg ctcagacaga aattgaacgt ttagatgaac
    20101 aaataaaaga aatcaatcga caaatgactg atttgaacga caataaagga ttcttgaatc
    20161 gtaaaaaaat cgaggaagaa attcaagaat tagagaagaa gaagcaagta ctatctaatg
    20221 aaaaattagg ctggcttcct tatattgaga cgcctgaaac aatccagcag caaaaagaag
    20281 cattgaagca agaagcaaga gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa
    20341 ggcaaatcag acgctatttc ggtagcaaag agggattcgg tcaagcaatc catgaattct
    20401 tgatgaatta tcttggcaat gagaatccta ataatcaatc taatgaagga ggttccgagt
    20461 atgaatgaac acgatgaagc agaatggaca aaaaatgaag aacaaacaga agatcatgaa
    20521 gaaacgaaca cttcattgga agaacaaaca agtaatcctc tggatgacac agaagaaatc
    20581 cctatgctag aggaaacaaa aatacacgaa aaagaaaaag taactccgac gaatacgaat
    20641 actgcagaaa acacccatgc ttacgaaata actgaaaatc ccgggtattt agaaggaaaa
    20701 gagcaagatc atcaagatat cgtcagtttg attgaaacag cggaagcaga attagcggat
    20761 ttacgtatga gaaaagattt gatcgaatca gattttccac aattattaga atcatatgat
    20821 cgttttatat tgaatgaaga accaatctcc acgatcggaa agacaagcct gatcgaatac
    20881 ttcacgtatg aattactgaa accgttgaat gatattggtc tggaacggtc aaattcagct
    20941 tatgacacat ggaaaacaag acatttttcg atcaattata cgttgaatgg acaaaacgaa
    21001 ttagtctttt cctttgtatt accgaccatc gatcaacgat accaagtgga atcgatgagt
    21061 ctcctaaacg tatcaccaga gacaatggaa atcaatgtcc aagatgaccg ggtgctttct
    21121 ttgatccgac actggtcagt ggatcgagta ttctctgcag ggcagatcac gatattcaat
    21181 cacaaattga atcaattatt agcccatgca agaacacttg gttttaccgt taatcagaca
    21241 ttactagaca atacgaagcc acttcgtttg aatcttcaga gcgaatttga attgacgaat
    21301 gaagtattgg atgatatttt tatcgtggca atgaaaaatc catcctacga tatggaaaag
    21361 atcaacgaaa ctacttatca agtacgatta gataaaggac agtctttaac gataacaaca
    21421 aacgaaaaag atcaaacagt cttgtcgatc tcatctggag actacccacg atctgtcatt
    21481 gatttcttta tcaattacga attcttggtc ccattaatgg tgagaaagtc ctgattggat
    21541 aagttggatt accagatgtc attcagataa ttctgttcca aaggacttgc ggcagttatc
    21601 cagaataatt gtcacaagtc ttttctgtat gtactaaatg gacgtgaata acttgctgat
    21661 gaaggaggga tttcattagg aatgtccaca aataaaatag cacagcttag cttattgtct
    21721 gctgcttgtg tagcaggtag gatcgctttt caatttattc cgaatttcca gccaatgact
    21781 gctatttttt tatttatagt actttactta agcttaaaag atgcgttaat tgtcatgagt
    21841 ctttctattg cgatcagcag tttttatttt ggtgttggac catgggtcat cggccaatgg
    21901 atcagctata ctgttgttct gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa
    21961 aggaatctat ggttaaaggg agtctgtttt tttctggcgg gcatggttta cggaataggt
    22021 atgaccgttt ttgatacgct gttatatcgt ttgcctcaac cttggatcta ttaccttcaa
    22081 ggggtgtcgt ttgatttgat gcacagtata ggcaatgttg tgttctttct tgtttttctg
    22141 ccagtcgtca aacgttttta caatcattca ggaggaaaaa atgaaaaaaa taatttatag
    22201 tttgcttgca atgagtgtca gtatactcat tttgacaagt tgcagtacaa cctatacaca
    22261 atcggcttcc aaccaaaagg aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca
    22321 agaaaatggt caaaactttt cggaaaaaga agtgttcttc aaaaaaggcg atgatttact
    22381 gagtattttg aaacgaaatt ttgaagtcaa agaagataac ggcttcatca cttcgcttga
    22441 agggcactca caggatgaga aaaatcaact ctattggatg tttactgtgg acgggaaaag
    22501 tgctactact ggtgccaaag agatcaaatt gaatgatgga caatcggtca ttttcaattt
    22561 gtctaaacta taagtttcat aaaaaactgt taaagggcga atattgccat aattcagtga
    22621 gttcagttga tgaaagaagg aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc
    22681 cgtaattgct gctctagggg ctaatattct agttgctatc agcaaatttg tcggctatgc
    22741 aatcagtggc agtgccgcca tgctaaacga aagcatccac agtatcgttg attgcagcaa
    22801 tcagattttg ctgttattcg gtgataaaca agccaataaa ggacagagcg agttgcatca
    22861 atttggtgaa ggacgagcaa aatacttttt cagtacaatc gtcgcaatga tgcttttctt
    22921 cggcggtggg gcacttgggg tgatggaagc ttttgaaaag ttgacgcatc catcccatga
    22981 agtgggtaat ccgatcattg ttatagcaat actgttattt ggtttggtaa tcgaaggaag
    23041 ttcgttacgc gtggcaatga aagaaatcaa agaattgaat actgaaaaac tttccacgat
    23101 gcgctttttg cgcgaaagtc gacacagtga aatactgatt atttttacgg aagacttgtg
    23161 tgctgtaatc ggtttggtct tggctttagc aggaacaata ttgacttatg caaccgggat
    23221 tgctgcattt gatgcgatca gcggattgct gatcggtttc ttgctgatgg gagccgccat
    23281 tttcctagca aaagaatttt atagtctgat catcggtgaa agcgtgacag caagcgattt
    23341 atcaaaaatc aagttagctt ttgaacgtcc tgagatcagt cggctgatcg atataaaaac
    23401 agttcattta agtccaacgg agattttagt tgcagcaaaa gcagatatcg ttggtaacaa
    23461 agagaatgaa gcttactcgg tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc
    23521 tgacaagaag atgtatatat acattgaaac agataaatat gacccaaatt attctcgtaa
    23581 ataaaacgtt tattcatgaa ttgaagcaaa aatgagaaaa atataatctt gtttttaaat
    23641 aaaacggcta caatttagcg aatttatgtt acaatgagtc cgctgtttat ttgttgtaat
    23701 taaaggagat attttaatga acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc
    23761 agtgatgcca ctttgtataa gctatatacc agtaggatta gcttgcggag tattgttgca
    23821 aaaagttgga tttgatcctc tcttatcagg actgttatcg attctggtct tttccggtgg
    23881 cgcgcagttt ttagttgcat ctatgcttac gacgcaagca tcatttgcaa cgacattatt
    23941 gatggttttc tttttagaat tacgctatac attattagga gcaagtcttg ccggttttat
    24001 gaaaaaagaa aagcggacgt ttttggctgt cttttctcaa tcactgaatg atgaaaacta
    24061 tgcggtgaac tacttaaagt tttcaactga cccttcctgg aataaaagaa aggctttata
    24121 tgtcaattgg ttctcaatga ctgcatgggc cggtagcaat atgatcggta attttttcgg
    24181 ctcggtgatt cgagtagatg ctgaccttgt ccattttgct ttgacggcca tgttcatttt
    24241 catgtttatc atgcagatga aaagcgcact actgatcttt acaggtttat tctcaggtgt
    24301 attaggggtc gtattcatgg tattattcca aaatactttc ggattgattg cagcaacagt
    24361 cattgcttca tttgccggat ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa
    24421 actaagagac cgcggtaaag atgtcaatga gccgctcttc aacttcccgg aacaggaggc
    24481 caatcaccat gactaaacta tatttgctac ttttagttgc ttgtctgttc atcgtggcat
    24541 atatcccgcg cttgttcccg atgctttatt ttactcatcg aaaagtgcca tcctggttca
    24601 gtgactggat gaaatatgta ccggtggcat tatttgcggc gttagctttt aaagatgttt
    24661 tcattactca tgaacatctg gatattgctt ggaatatcaa aatagctgca atgattctag
    24721 tagcaggcgt ggcttacaaa acacgttcga tggcgttgtc agttttgaca ggacttgcgt
    24781 ctgtcttttt gctgtctatg ctttaaactg ttttatttta ttaaaaacag agattgcgac
    24841 ataagttgtt caaactgatc tatccgaact gcatttgcaa cagtcaattc tggatgcaag
    24901 tttattcgta gggataaaaa agattagtgt tggcttatga tgcaagctct tttttatttt
    24961 aggaattaga gagggatttg tccttctatg attttttgct ataataggga agatgtttat
    25021 tttatgaaag aatagttgag ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt
    25081 ggctaacctt tgctaatctt ctatgtaaag ttttaggggt agtctatttg attccctggc
    25141 taagtatgat ggggaacaat caagatggta tgcttgctac gacattgtat aatgttggct
    25201 atcttcctta tggattgttc ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa
    25261 aaaaagtagc tgtagccaca aaagaaggag ataacgctgc ttgtcggctg atttttagaa
    25321 gtacgatcaa tatcatgttt gtcatcggta tcgcctctgc tgcactgatg tatttatttg
    25381 ctcctttact agccggaatc agtccgattg ctaacgttaa taatgggata ctagctatca
    25441 gaagtttatg cccatctttg attgctatcc caattcttag tgcaatgaga ggatatttcc
    25501 aaggaaaaaa tgatctgcgg ccttatggaa cttctttgat tatcgaacag gctgtgcgag
    25561 tgatcgttat cttggctggt acttattact tgcgcgtatt gactgacgga accatattag
    25621 aagctgtatt gatcagtaca gtcgcttctt tcttcggtgg gttagctgcg attatccata
    25681 tgtatatcgt cggacgcaga aaagacttct tcgaacttaa agattttttg atttccagcc
    25741 gttatttcga aagagaaaac cgatctgcct ctgtctctat cattcgggaa acactaccgt
    25801 ttatttttgt cggctcagtc attactatcg ttcaaatgat cgaccaagta acgatgaaac
    25861 cactgcttca ctttttccgt ccagagatcg cagatcagca actggaattc ttattcagcc
    25921 gtgcttctgt caatccaaat aaattgacat tgatcctgat ctccatggta ggaacagtag
    25981 ctataagcag cttgccaata ttaagcacga tgaagaaaaa agatcggata caaatcgaaa
    26041 aaacagtagg agatagtttt tcgattgcgt tattgattct tcttccttca ttgactggaa
    26101 tgtctttatt agctggacca ctatacacat tgttttttgg ctatgatcct gaaagtgtcg
    26161 gatatttcca aatggctttg ctagcttctt tgtttttctc tttattcacg atcctatcta
    26221 cgatgcttca atctttgaat caccatcgtt ttgcgatccg attaactgta gaagcaatta
    26281 tcctgaaagt catcttccaa gtaatcggat taggattagt tggcggatac gggatgagct
    26341 tatcaaatgg cttagcattc ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag
    26401 aataccgaat agcaccttta gcaaagatca gcaatttttt cttaaaaact ttccgctcca
    26461 ccctgattat gcttgcagta tgttttgccg ttttcttttt gctgaatcag cgattgtcaa
    26521 tggaatcaaa aagctatgca atgatttatt gtgtggctgt aggaagtatt ggcggcttag
    26581 tatttttatt ttctcaattc ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc
    26641 atagcagaag caaagaatag gttaactaat cgttttttgc tgtcacttga aaaacggcaa
    26701 ctcgtatgat tacgcttgtc gtttttctat tttccaattt tagtttcata tctaatctta
    26761 cttatgatat agttataagg tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga
    26821 aacttgcagt agttacagat agttcggcat atctgccgga acgtatcaag aaccatcccg
    26881 acttattcgt tattccaatc cctttgatta tggatgggaa aatttataac gaaggaatcg
    26941 atatcgaagc ggatgaatat tatggacttt taaaaaacag taaagatttt cctactactt
    27001 cacaacctgt cgtaggggaa gttttgggtt tatatgagga attgaaagag aaagggtatg
    27061 acacgatttt aagtatccat ctttcttcag ggatttctgg ctttgtcaac acattatttg
    27121 cgatgaaaga tgacatcagc ggagtaacgg tcattcctta tgactctaag atcactagta
    27181 tgccgatggg acacatggta gaagctgcct tagacttaaa tgatgaaggt cagtcccttg
    27241 atgaaatact tgcacatatc gatcgaatcc gagataatac ctacgcttat ctgattgtag
    27301 atgatttgaa caatttagta cgaggaggac gtttgacaaa tggtgcagcg ctgattggca
    27361 gcttattaaa aatcaaaccg atcctgacat tttctgaagg gaaaattgtt ttattcgaaa
    27421 aaatccgttc aagtaaaaaa gcatttgctc gagctgaaga aattattggt agaagaaata
    27481 acgaaataca accgccagtc aaactttacg tgatacatgc aaataattta gaagtagcga
    27541 ttgcggagaa agagaaattg cagaaaaaat atccaaatgc agaaatcgaa attggtcatt
    27601 tcggaccagt cattggtaca catttaggtg aaaaagccat cggcattgcc atttcagcgc
    27661 aataattcct tttattcaaa aaacatacca aaaatattcc aacatcatct tggactttct
    27721 acatgttact aatgagcgtg taaaagaacg agatggtgtt tttatttcta tcaatgaagc
    27781 tctttttctt tgcaatcctc acttcaatcc tctataatgt cgcatatagt ttagtaaatg
    27841 agtaaactac taatttactt atttactaaa aataaaagga tgaggagtta aaataatgga
    27901 aataccaaca aatttaaaac ataaaccagt aatcgttgta gaagactatg ataaagttga
    27961 tggaagaaat gcattaaata cggatgcaaa agggctttca ctgggccttg ctcagtggaa
    28021 tgatcgtgga aaagtcgata tctcaggcaa ggtatggcga catactggag aaaaatggtc
    28081 acgacagtcg gaagaattac caatcacacg tattttagat ttagcgatct tagctgcaca
    28141 aggaagttta tacttccaag atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc
    28201 agtcgtagac atcattggat tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa
    28261 tccaaagatc gatgaagaca tcattttgta ttacgataca ctacaaaaag acggagaact
    28321 gattggggaa cgattccgta ttttaaaacg tcttttagat gagctaggat attaatagac
    28381 agagatgatc aaaaggggaa gagaagcatg gagaatttct cactaacaga aaaccgaacc
    28441 ttttcacggc tagaaaagca aatgatctgg aagaaaccga aaagccatcg gacaagtgca
    28501 gaagaatggc ggatcgcctc cgaaatcaaa gggaattggc aagatcctga aatgaaaatc
    28561 agtaacgtac ttttggaagg agatgccggg tcggggaaga cacaattagc caaagcacta
    28621 tctgctgatc tgcaacttcc ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca
    28681 gatgttttcg gagccttatt acctgtagta gattctaata aggaacaaga ccaagaatta
    28741 ctagaagcta tttaccaaac agatacactt gaagctgtac tgacactgat ccagaaccat
    28801 tacggcattg atcctttttc tgctaaagaa aaattagcag agctagttca aaggatcgaa
    28861 gctgattatc cagatgcaat ccattacagc tattaccctt ctgaaatcgt tcgagcgatt
    28921 gaaaaaggat atttattaga gattcaagag ccgactgtca tccgagatgc ctctgttctt
    28981 gtggcactta actcagcgct cgaaccaaat gggatgttga atttaccgac aggaatcatt
    29041 cgtcgccatc cagattgcgt cattgtgatc actacaaatc gaaattacca aggaaatcgc
    29101 ccattaaatg aatctttaag agatcggatg caacatgcgg aaaaaatgga tcttcctgcc
    29161 ttagaagtca tggcagaacg tgcgatggcg aaaactatga tccaaaaaca agaacttctc
    29221 ttaaagatgg cagagattat ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa
    29281 ggtgtagcag gaatgcgttc ttatttttat tgggcaaata cctataagca aggacaagat
    29341 ttactccagt cgatttatcc aaaagttttg tataaattat ccactgattc agatgaattg
    29401 gcaattcttg aacaagcttt gtctgacagt ggattgctac tggaacttca ggacatattg
    29461 agaaaagaaa aatggggtca tcctcaatct gagcatacaa aaggaagaac cattagcgcc
    29521 gaagaagcaa atgaacgaaa catcactgaa aatgctgaag aaattctgcg cttagatgaa
    29581 aagaaatcgt tgtctgctga agaaccaaat gagggagaag agaaacaaga agaaacaata
    29641 gaaaaaaacg agattcctcc tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa
    29701 aatcaaaaag aacaacaaac aggagatgat tctcaagaaa tgtctgcttc tgacatggaa
    29761 gcacatgata aggaaatcaa gaagcaactg aataaagaag cgcgacaaat catgaaggaa
    29821 acgattcatg aaaaagaagg tctcatcgtt caccgaccag ctttccaagg aaaaacgccg
    29881 gaagcacgtg agctacacat gcaagtaatg ccaatagtag aatcacttag ccggcaaatt
    29941 ttagagttac tggataatga acaaacagag acgtaccaaa aaggaaaata cgaaggtcag
    30001 cggttcaatg caagcaaagt agcatatggt gatttgagaa attttgacaa aaagaacccg
    30061 ccacatgagc aaccttcttt agcggtagct gtacggatcg atgaatctgg gtcaatgata
    30121 cgggatgatc ggatcgaagc ggcaaaaaaa gcagcgattg ccattgccga attttctaag
    30181 aaagtcgata tcccattgtt gatttacgga gatacagcag accgttcgtc aagagaaaag
    30241 acttctttgt tttcttataa agaatttgaa gacggatttc aatggttaaa tgaaaaatta
    30301 gtcactatga aaccaaggca aaacaatcgt gatggtgctg cacttcgttt agcagcagaa
    30361 aaattaaaca gacaatccgc aacaaccaaa ctgttgctta atattagtga cggtcagcca
    30421 aaagcgttgc cggattatac aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa
    30481 gaatatgaac gtcaaggaat cctatttgtc tccgcagcta ttggccaaga caaagaagaa
    30541 atcaagtcga tctacggtga aagtcgtttt gtagatatta ctgacttgaa tgaatttccc
    30601 aagcaaatga ttcaactgat tgcccgctat ctatgataaa ataaagctca acgaacgcac
    30661 ttagcagatt cgttgagctt ttcgtttcta gttaccggta acaaggatac ttctaaaata
    30721 gaatcggtta taatcaagag ggaccgttaa aggagtgaaa cgatatgata caagaacaaa
    30781 aatggtggaa agaagcagta ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg
    30841 gcgatggtca aggagacatc aatgggatcc gtgaaaaact taattatttg aaagatcttg
    30901 gtatcggctt tatctggatc aatccgatct atcaatctcc atttgtcgat aatggttatg
    30961 acgtaagtga ctatcaagat atagaagaga gattcggaac gatggaagat tttgatttat
    31021 tgctaaaaga agcacatgaa ttagggatca aagtgattat ggatcttgtc atcaaccata
    31081 gttcggatca gcatcaatgg tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg
    31141 attattacat ttgggtcgac ggggtagatg gcaaagaacc gaataattgg acatcgattt
    31201 tcggcggttc tgcttgggag tattctcatg aaactggaca gtattatctg catgtttttg
    31261 caaaagaaca accagactta aattgggaaa gcgagaaatt gaaagaagaa ctattcaata
    31321 tgatccgatg gtggttggat aaagggatag atggatttcg tttagatgcg atttctcatg
    31381 taaaaaaaga tgaatattca gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg
    31441 tctcaggaat cgaagaacat ttgaccgatt tgaaacatgt ttttgaagag tacgatatca
    31501 tgactgtcgg agaagcaagt ggtgtcactg cagaagaagg accacaatgg gtagggaaag
    31561 acggttattt cgatatgata ttcgaatttg atcatatcca tatctggcag caagaaaaag
    31621 aagggcaatt agatgtcctt aaattgaaac ttgcattaag cgcttggcaa acttcattgg
    31681 atggtatcgg atggaacgct ttatatatgg aaaaccatga tgttcctcgt gccgtttcag
    31741 tgtttggaga tactcgtccc gatttttggg ctatgtctgc aaaagcaatc gctatgatgt
    31801 atttcttttt gcaaggtacg ccatttattt atcaaggaca ggaaatcgga atgacgaata
    31861 tgccctttga atcgatcgat caagttgatg cagtagattc gaaaagactt tataaaaggc
    31921 tgcttgcgga aggtaagaca agagaagaag cattggatat cattcgtgaa acaacgagag
    31981 acaacagcag gacacccatg caatggactt cggaacaata tgctggtttt tctacacatg
    32041 aaccttggct gatcactaat ccaaatacca aaacgatcaa tgtagaacaa caagaatatg
    32101 aaccagagtc ggtcctgcaa ttctataaga acatgatccg cattagacaa accaataaag
    32161 gtctgatata cggttcttat aaagaatatc ttcatgaaca tccacaactt tatgtctatg
    32221 aaagatattt agaagatgaa caatatctta ttatggtaaa cttaacagaa tcacttgctg
    32281 actatgaact tccaaaagaa gcagatcagt cctggacatt actcttgtca aattcctcta
    32341 gcggagaatt tgaagctaaa ggcatactag caccttatga agctcggtta tataaaacaa
    32401 acaagtaaaa cttcctcgtt aaagttcatt agttggactt tgcgaggaag tttttcagat
    32461 tgctctgtca attactcatt tcaataattc gctctaggac caccgatata tttaggacga
    32521 gagcgaaggg ctgtcacgtg agcttgtcca agggttttca aacgaggtct gacaggtact
    32581 tggacatgtt tgatatgcat accgattgat gtgtcaccga tatcgatacc ggcttgtcca
    32641 actacgtgtt ccacttcgac aggatcattg aactggtcaa aagcagcaac agaacaagca
    32701 cctccagcat gtagtgcagg aacaacagag acaatttcga aattttttgc ttctgccact
    32761 gttcgttcta ctactaatgc tcggttcaaa tgttcacaac cttggacagc tagcgcaatt
    32821 tctttaggat ccaacaactc ttttaacgta cggatgatcc attgaccaac ttccgcactg
    32881 gaattttttc caatatgtcc tccaacgact tcgcttgtgc tgcatcctaa aacaaaaaga
    32941 tcaccttttt tcaagtgtgc ctcagccaat atgtcattaa ctatttcagt caattggtct
    33001 tttaattctt tttctgacaa cttcattttc taaaaccttc tttttcaatt aatattgctt
    33061 ccgtgcttga atacgttgaa gacttgcagt tataggaaag gcaagtccca ttccaatcag
    33121 attttggaag atgtttcctg gtatagaagc cactccagcc ggccaatcat ataaaatcca
    33181 accggcgata ccatagccga tgatcataac taagcttcct aataaaagcc ctaatcccca
    33241 catacgagta gaagatttgc tgaaagaacc agcaacatat ccttgtaacc cgtggatcac
    33301 taatgaaaaa actgcccact gcggatatcc tgaaatcaaa tcgatcaata gaccactgga
    33361 agcaccaaca actaatccac ccgttggacc ataaagaaaa gcagcggtat agatcccggc
    33421 ctcgcataat gtcacaaatc cattcgtagc cggtacaggg atcaaaattg ttaatgaaag
    33481 ggcaactgtc aacgcaatca gcatagcata gatcgttaat tgcttagttt tcatttctca
    33541 ttcctccttt ttgaataccg ccagaccaga cacttccggt atgatcgttc atcagcacgc
    33601 cattagaaat acaatgggca acaaattttt tactttctgc tactgctttg gtaaaatctg
    33661 cccctaacac aagattcgct gttattgcag aagaaaaggc acaaccggcc ccgtttactg
    33721 ttgtagtgtc gattttttta tgagaaaata cctcataatg atcaattgaa tagagaagat
    33781 ccacagcttc atctccagcc atccttgtgc cgcctttgat cacaatgttt tttgttcctg
    33841 aagcagtgag ttctttagcg agatggatca tttctgttaa cgaagaggga agagcttgac
    33901 cagaaagtaa agcagcttct gccaaattag gcgtaatcat cgtaacaagt ggaaaaagtt
    33961 cttcgatgag ctgcgaaata tacaattgat tgatcacagc ttcagtttct ttgaatgcca
    34021 aaacagggtc taaaatgatt gggatatttt gttttgtttt tatgaagtct cgaacaacca
    34081 tgattgcttc acttgaattc aataacccaa ttttgatacc atctaatgaa gtcattttat
    34141 caatcgaaac taactgtcgt ttaagcaatt ccaaagatat agattccagc ataaatcgct
    34201 cattaatatc tagtgcccct atagaagtca aggcgcttag tccgaataga ccaaaattct
    34261 caaaagtttt taaatctgtt tggattcctc caccagcaaa tggatcagaa ccgccaattg
    34321 tcaagattct tttcataaca ttttcctcca tgaataagag tataacgaaa ttttcaaaaa
    34381 agaaaaacag ccaattggca tatttttatc tcatccaatt taaaaaaaca aaaagaaaag
    34441 aaagaccaaa atggatcttt ctttccttaa atagttagga cttttgtgat tttaattctt
    34501 tctcgatttg aagccgttgc actttcattg tagctgttcg cgggattgca tcaaaatcac
    34561 ggatgatagg gacgttcaag cgaggaagtt catggacttg ctcccaccat cgattccaat
    34621 ccatttcttt atcaggagct aaagcaataa tcggttgtgg agaatgattt ttatccctta
    34681 caatcactac ttctgccaaa aaatctaacg aatctaataa aaagtcttca atagctaatg
    34741 tactattgat cgtttcgatc aaatccactt gccgatcttt caaaaatagg tggccatgct
    34801 tatccataaa accgtaatct ccgctatccc accaatcgcc ataaacattt tcctggaacc
    34861 gtgcatcttc cttgtaataa gtcaaagccc gtcctttaga cagtaattgt atatgaccat
    34921 ctgtgttttc aggtaggaca ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt
    34981 ccagtccaac gcccatatct cttgcatcac ttgttttcaa actctctagt gtatgagcct
    35041 taagaatcat tggtccgcat tcactttggc catatacttg aagaaatacc gcatcctgtg
    35101 ccatcgacgt tcgcaaaaat gtcttcattg tctggttgtt tatagcatca aaagtggagt
    35161 gatagtatcg gatacccgaa aaagcttctg gatgctcctt agcagtaaaa cgccactgaa
    35221 caaagttatt cggatgggtc tctagtgcaa ttggccggtg agcttccaac atatgaacaa
    35281 cttttgaact ttgtgcgttt gctaaaggca tcataggaaa tcccatggac atcaaagaag
    35341 agataccaat attgaatctg gaatgcactg gagagatgtg aaaagcgatt aatttctttt
    35401 ccgaaatttt tgtgaaaact gttttttgcc atttcgtccg ccatcccata gaatgagcag
    35461 aatgacaaat aagtttaggg ataccagttg taccagaagt atgggtcata taagaaattt
    35521 catttttgtc taaaaatgat tgagaaacag acaaagcagg ttgttccatt aaataaagaa
    35581 tagg
//
LOCUS       DAFEHS010000020        35201 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFEHS010000020
VERSION     DAFEHS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.020"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..188)
                     /locus_tag="LP327_001078"
     CDS             complement(<1..188)
                     /locus_tag="LP327_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725211.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGDI
                     LTLNLTTKTFVDAAEDYP"
     gene            complement(444..2036)
                     /locus_tag="LP327_001079"
     CDS             complement(444..2036)
                     /locus_tag="LP327_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725212.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
     gene            complement(2056..2478)
                     /locus_tag="LP327_001080"
     CDS             complement(2056..2478)
                     /locus_tag="LP327_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725213.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            complement(2496..2783)
                     /locus_tag="LP327_001081"
     CDS             complement(2496..2783)
                     /locus_tag="LP327_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725214.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            complement(3240..3470)
                     /locus_tag="LP327_001082"
     CDS             complement(3240..3470)
                     /locus_tag="LP327_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725215.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTAERK"
     gene            complement(3492..4298)
                     /locus_tag="LP327_001083"
     CDS             complement(3492..4298)
                     /locus_tag="LP327_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725216.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIISEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            complement(4309..4953)
                     /locus_tag="LP327_001084"
     CDS             complement(4309..4953)
                     /locus_tag="LP327_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725217.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQANIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDNHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            complement(4969..6171)
                     /locus_tag="LP327_001085"
     CDS             complement(4969..6171)
                     /locus_tag="LP327_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL1725218.1"
                     /translation="MENDTNIVMEHLKRRAKRRLFLWLFGTSAGLITILITVFVTLFL
                     ILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKL
                     LPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFP
                     NSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMSI
                     ITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGGE
                     FRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQ
                     EFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPL
                     PFLNSSKK"
     gene            complement(6245..6580)
                     /locus_tag="LP327_001086"
     CDS             complement(6245..6580)
                     /locus_tag="LP327_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL1725219.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
     gene            complement(6580..6843)
                     /locus_tag="LP327_001087"
     CDS             complement(6580..6843)
                     /locus_tag="LP327_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HBL1725220.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            complement(6902..7279)
                     /locus_tag="LP327_001088"
                     /pseudo
     CDS             complement(6902..7279)
                     /locus_tag="LP327_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            complement(7410..9287)
                     /gene="ltrA"
                     /locus_tag="LP327_001089"
     CDS             complement(7410..9287)
                     /gene="ltrA"
                     /locus_tag="LP327_001089"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL1725221.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            complement(9975..11669)
                     /locus_tag="LP327_001090"
     CDS             complement(9975..11669)
                     /locus_tag="LP327_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBL1725222.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            complement(11832..12425)
                     /locus_tag="LP327_001091"
     CDS             complement(11832..12425)
                     /locus_tag="LP327_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725223.1"
                     /translation="MRVKGQNSVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(12426..12758)
                     /locus_tag="LP327_001092"
     CDS             complement(12426..12758)
                     /locus_tag="LP327_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725224.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(12810..15188)
                     /locus_tag="LP327_001093"
     CDS             complement(12810..15188)
                     /locus_tag="LP327_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725225.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMTDKDKSSLSDLQKDRLRALQYRLELDDNGKEVPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNI
                     NSENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKIGLPKAQNLPMKDSRL
                     NQFKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREA
                     APEPIQRVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP"
     gene            complement(15185..17797)
                     /locus_tag="LP327_001094"
     CDS             complement(15185..17797)
                     /locus_tag="LP327_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBL1725226.1"
                     /translation="MRIRETTKRKDKPFNLEKKKYLSHFYRYVPAGERLPGKHDKRVK
                     QKLMYSGLVYLPLDYLVTRIPEAFSRFEIIQGSLQGVNLFQLQEMPQVFHFDSAGFWN
                     PLTPATSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPDTKGSFPGYGGIPITHYKKYWYIQTDTVNTCIIGTSRSGKGQIEVLATIDNLSRAE
                     KQSSLVVNDPKNELYVASKDELEARGYDVYAYNLTEPLQSMSDNPLALIVKYWQRGDV
                     DTATQLANTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYTKLTPNN
                     LVELLSEIGGFNYQDPTNEFKQLNALDEFMNQLPPGNLAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRIGVEFYHDGKK
                     FHEERVKVGYKEFCEINFDCHLEQGDQVCLKYDHQGKEYVAWYEFSQKVLKDEKGNIV
                     YRKKRGETHLPEWEKEAVLTLDESKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYKQLRAKYGEDNADIIKENCQTHIYIMSSNTDTIEEFSQNVGNKTVEQENST
                     INALGMNTHINRNVDSDRILPPERIRSLLKGETIVLAPLKREDLKGNKIRPYPIFNSE
                     KTNSPYAYQYLEDFDTLGRTIHDIDIYCTHADLDLKALQIDYDYFLPDEDARDLYHGL
                     HEQLEEKIEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNEEISEII
                     GKRLLLEADKAVRE"
     gene            complement(17797..19854)
                     /locus_tag="LP327_001095"
     CDS             complement(17797..19854)
                     /locus_tag="LP327_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725227.1"
                     /translation="MYFTIAGNTKKDKELIKKATKEVGKRTSFLPKTMKYSVIETEEQ
                     LHVLGKVLEQESQSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     DESKEKETDNVDEQADIPTQKSIFEEENEVMDQLDGDPFEETMEETTEVEIDPVEANE
                     EPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNQLFLNE
                     LYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEERQQ
                     ETDQEINEYAAEQSSNWEKAKEELNKEEQQVIEEEVQKIKERYEKKRDNAYSLCVEKI
                     NNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTTTE
                     VLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQDLA
                     EQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQLQPMPSVAAVQTPMNN
                     QTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQ
                     EQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKTKEGSSDSTVQSNSSESSESN
                     EVK"
     gene            complement(19923..20225)
                     /locus_tag="LP327_001096"
     CDS             complement(19923..20225)
                     /locus_tag="LP327_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725228.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAGVAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGIILAAASIVVSLFGMFGQTPQGL"
     gene            complement(20243..20443)
                     /locus_tag="LP327_001097"
     CDS             complement(20243..20443)
                     /locus_tag="LP327_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBL1725229.1"
                     /translation="MAKAKTPFEKAASKILERRGVSETQWKQEVLEKAQFDLLRGKDK
                     DLEGYIYQREREKLVFDEIIKH"
     gene            complement(20483..20949)
                     /locus_tag="LP327_001098"
                     /pseudo
     CDS             complement(20483..20949)
                     /locus_tag="LP327_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21138..21344
                     /locus_tag="LP327_001099"
     CDS             21138..21344
                     /locus_tag="LP327_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725230.1"
                     /translation="MYADKNEIEWLLFESGISVKDIYMESGVARTTVIDLINKTSSID
                     KMRFDNAVKLTKMAKMRKNLYENK"
     gene            21484..23550
                     /locus_tag="LP327_001100"
     CDS             21484..23550
                     /locus_tag="LP327_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBL1725231.1"
                     /translation="MKLLNPKTVIAGTFAVTLAATLSLAGSVDAASTSVMYRLYNPNN
                     HEHFYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEV
                     KWLTKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWK
                     YEGIGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDS
                     KATQKQVDSAKNTLDSAYKGLKKKPAPTVNKAELQALYNRLKSTAKGDFTEESWNNFQ
                     TALSNAKKILDDSKASQAQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLL
                     KEEHVSVKAGTSYTAKAKTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVE
                     VKARPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPTFTNFVLDPRESSSNTVTLN
                     NVTSNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDNFSTSWKNMTDQNYFL
                     CRNDDSSVTVDKNGQRIINHVDQNRTITFKYKNISLNDLNNYVRQKELAWLNSYRQQN
                     GVAPMQFNDVVQKAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYL
                     YVDEILSDTGAVNAMIMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFIS
                     ANPIFK"
     gene            complement(23586..25676)
                     /locus_tag="LP327_001101"
     CDS             complement(23586..25676)
                     /locus_tag="LP327_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL1725232.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVSGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNIL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHATQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVECGSNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASANFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTTLVVSLLL
                     LVLATFSYVVLKFN"
     gene            complement(25673..25933)
                     /locus_tag="LP327_001102"
     CDS             complement(25673..25933)
                     /locus_tag="LP327_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725233.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQPYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVVVGISLVIYKKEGMKG"
     gene            complement(25946..26707)
                     /locus_tag="LP327_001103"
     CDS             complement(25946..26707)
                     /locus_tag="LP327_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725234.1"
                     /translation="MRNRKWLFVCAAALLSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKNLTTDQIESQLKDRVKKLSSDQLKLVSNGETKTIGQQT
                     GVFEFSVEVQANQKQAYYIVNETSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(26723..27475)
                     /locus_tag="LP327_001104"
     CDS             complement(26723..27475)
                     /locus_tag="LP327_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL1725235.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAAGVLLLMPAEASVAKYQQNQ
                     QIVAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEKN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(27490..29466)
                     /locus_tag="LP327_001105"
     CDS             complement(27490..29466)
                     /locus_tag="LP327_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL1725236.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVTSETSPYSVFKWGDTA
                     GQGLSFVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYFGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(29519..30190)
                     /locus_tag="LP327_001106"
     CDS             complement(29519..30190)
                     /locus_tag="LP327_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL1725237.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(30272..31438)
                     /locus_tag="LP327_001107"
     CDS             complement(30272..31438)
                     /locus_tag="LP327_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBL1725238.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(31444..32133)
                     /locus_tag="LP327_001108"
     CDS             complement(31444..32133)
                     /locus_tag="LP327_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBL1725239.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(32260..32499)
                     /locus_tag="LP327_001109"
     CDS             complement(32260..32499)
                     /locus_tag="LP327_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725240.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(32566..32805)
                     /locus_tag="LP327_001110"
     CDS             complement(32566..32805)
                     /locus_tag="LP327_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725241.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(32919..33260)
                     /locus_tag="LP327_001111"
     CDS             complement(32919..33260)
                     /locus_tag="LP327_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBL1725242.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(33247..33576)
                     /locus_tag="LP327_001112"
     CDS             complement(33247..33576)
                     /locus_tag="LP327_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725243.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(34167..34499)
                     /locus_tag="LP327_001113"
     CDS             complement(34167..34499)
                     /locus_tag="LP327_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL1725244.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
BASE COUNT         9810 a         7199 c         5458 g        12734 t
ORIGIN      
        1 aatggataat cttctgccgc atcgacgaac gtcttcgtag tcagatttaa cgttaaaata
       61 tcgccatttt gatacgcttt tgccgctttg tctgtgacga caaaagctct gcctgtttga
      121 atttctgtca caaaaacgag attgtctctg cttttttcaa caacaactcc aatcgttgaa
      181 taagccattt cttcttcctc ccttctcttt cttttgatat aatgtagtcg caaatcgcac
      241 cactcattga attggcggaa aacaattcat tgaaaaaaag agtcctggac aattacgttc
      301 aggactcttt tacttctttt attcacttaa caactaacat tctgtgagtg aaatagactc
      361 ttcaatgctt ttacacggga agttattgat cccctcttca ttcatttccc aagtagtata
      421 ataaggcgct aactttactg tctttactgt ctttcttgtt ctatttggat tcttcgttgt
      481 tgctcttcgt aggctttact tgcgtggtat ttttcttcat gggcattaat cgttcgattt
      541 aattgacgca aactccgttg cgtgacaact ctttgttgga attgtttcct gcgcttcatg
      601 aattcttcgt aactatattt gtattcattc gttggtaact gtttaagcgt tcgaaaggat
      661 cgttcattgt aaatagattg catctgatcc atactttcta ttaaaggttt tatttgacta
      721 ttctgtttct tcagttctgt tagaatggca tttcctgtgc gctttttcaa atcaaagaga
      781 gcattttttt ctgcatcctt tgattctctt ttgctttccc cataaagact ttttgcttct
      841 tctttcaatg cttttgaaat ctgtatatat tcttgatatt caggttcttt tgttaaaaca
      901 tatttggtta actcatcgag tttctctctt gtgtatggtg aaagtttggt tcgtttttca
      961 tccccataat gccattccga acgtttgtca ggtagggttt tatatacttc atttaataag
     1021 ttcattgcgt gtcgatcgta acgcgctggc tcttttacca cttttgttaa ggtgttacgc
     1081 aaatcacttt tccgcgtcaa taaatttcgt ttctccatac caatcattcg atccttttca
     1141 taagaaaaac gttctaactc atgtacataa ttggtcttca ttgcgtctaa tgttgcttgt
     1201 cttctcttac ctttcggtac gatataactc acaccatttt caacctccgt gtaaggttta
     1261 cgcgtatttt ctttttcaac aactgcaaaa tgaatatgga tatgtttggt attacggtga
     1321 atcgttgcca tccagtagcc tgtttcgttc agttgctctt tctcgaataa tgtctccatc
     1381 atttttcttc cagctgcttt taatccttct tcatctaaag tatctgtttt gggatcgtac
     1441 aatcctattt tttcaagata cgaattatca tgagagacaa catcttgata aatgattgag
     1501 cctttttgtt gtcctctttg gaagatttgc ttaatctcct tcagatcact tcctttcact
     1561 tcgtctgaag ccatagaaaa aactccagga aggtttcctg aagctttttt gtgtggctct
     1621 gtttgatatt tttttattgc ttggttcaag cgttggtatt ctttttgttc actttgcgtc
     1681 agttctttct tttgttctaa cgcatagtga cgggtcatat aagaaagata tttggtaaaa
     1741 tcttgttcct ctaacttgtc aaagttgatt ccatttccca tcaaagattg agcttcattc
     1801 gacgtttcgc tgaattcttt atccgtctta ctctccgaaa aagtttctcc ttcaggaata
     1861 taaaaatcct ctaaagcatt cttgattcgt tgcagttcct gttcttcttc aggcgtacgt
     1921 cgttccattt cttccagcgc tttttctctt gtcatataat caatgtatgt tgaaaaagat
     1981 tttttattgg gcaaggtaaa ttgacaagtg agaatgactg caggcgattt attcatcata
     2041 ttccccttct actttttatc tttttaaaaa ctttgttgga tcaaacgggt ctactctggc
     2101 atgaacagat tcatttattg atttcttgat tttaggagaa tacttcttcg tttgattcgc
     2161 ttttattttc ccttccacac gctcttttgc ttcttgatag acagttgaat ttgtaccaat
     2221 taatattcct gtatctttca aatgaagatc cgcaaactct gaaaccattt ctattaaaat
     2281 agaaatttcc tttcccattg cgttcaactt tgcgttcgtc atattattat ttttttcaat
     2341 ttctggatcg taattcatac ataaaaatcg aatggcttca gaatagtttt ttaccgtagg
     2401 ctgagtttcc ataatatgtt ttaaaacttc atcgtgcgct tcataaaatg taatatgctt
     2461 ttctatcgct ttaaccatag aacattatct ccttcctaat gtgcgattaa tatttcaatt
     2521 aagttcttga cctcgttcgt gtatgcttcc aaaacttttg tattatgcag ctgaacatct
     2581 gtaaattttt ccaataaacg atcaaattga tctttcttgt agtccattaa aggacgttca
     2641 aagtcgtttt caatcaatag ttttagataa tcatttcgac tccatttttg accagtttgt
     2701 tctgttaatt ctacgcatct atgatcaatt tcttgaacat attttttatt aatacctctt
     2761 actttaatct ctttaatttc cattttcacc ctccgaaaaa agtatggata gtctatggag
     2821 tctatccata tgctatccat gtatagctgt tttactatcc atacactatc cataaatcga
     2881 atattttgta cccacttttg ttttagagaa acgtaatagg cacagcctat tggaacactt
     2941 tttttaaaaa gtgttcccac gtttccttat gctctttaaa aattaaggtt atttgtgtat
     3001 ttagacaata aaaaatcctt ctaactttgt cgattagaag gaaatatttt gtcatatgaa
     3061 aaatgaattt aagaaaaagt gtatgctata attgatacgc tgtcttcctg tttggttgaa
     3121 atcttcagcc agaaaggagg agtgttaaat gttttatgtg cttgatgttt gtgttacggt
     3181 gatatagctg atccataaac atttttgata tacttttttc acataaaatc ctcctgtttt
     3241 tatttccttt cagccgtaaa atatttttta gcagctacat atagagcttc aaacgatctg
     3301 aattttaaag tatctaaatt ctttcgacca ttcttcaact cagatattgt tgtgttagat
     3361 aaattcactt ctcgacatat ttctttagct tttacaactt tgagataggc tttaatcaat
     3421 actttttgct cagcgaaagt atctttgtca gccaagtttg ttcgcttcat tttttcactc
     3481 cacctgtcct tctaaatctt ttcttttgtc atacgatcaa ttttttcttg aagtttttga
     3541 atgatctctt cttgatcttt cagttgcgtt ttcgatgttt caatttcttg ctggtagcta
     3601 ttaatcttgt tttcagtatt ttcctgatca tctgcgactt gataaggcaa ttgatctttt
     3661 agcttcttca ctaattcttc attcaaatca atggttgctt ggaaagattc catttgtttt
     3721 tgagctgctt tttgttcttt cttgtatcct ttcactttgt aattgatcgc gtgttgttca
     3781 taatcttcat ctttcacttg atctttaacc ttatcttcgt gcacgtaata aatcagatct
     3841 tgtggttctt gcatatccac ttttggatca attttttcag aaataatggt taaacgcatg
     3901 gcgatatatt cttctggtac atgcgttagc ttcactgcaa aataatcatc agacaccttg
     3961 atcactttgg ctcgtattgc ttgataatcc cctttagctg tctttacttc tacgcgataa
     4021 ttaagattag ccagttcttt taacaagctt tgatcttcat cgtcgatcgc aaattcaaaa
     4081 actaattgct ggttcgctcc tttttctttt gaaaccattg tcagctcttt cgtgtttaaa
     4141 aaggcgttcg tgcttacctc atctggtata tctaccttct gattcgtata aatcggacta
     4201 gcaagaatca aaaagccgag aatcataaag attcccagcc attgaagata actttttcgt
     4261 tttttgaaac gttgtctttc tccttttttc ttactcattt tcttcctcct aacgagtgaa
     4321 ataactaaag tctatgactt tccattgacc atctacttgt ttttcagtga cagttaatcc
     4381 accttttatt ttttgtggtt tgacatcttt gaattgcgga ttgccgattg tataatcaac
     4441 aaatacgaca aaattttgtt cctcttttac actgcttgtt tgagcgtaag tttgcacgtc
     4501 acctaatgta aaggagtaac ttccgtaatc atcatgttgg tgattatcgt ctgcaggact
     4561 taatttgtcg atcacttctt gggttgcgac atcttttagt ttttccgtta attcatttct
     4621 gcttaatgat ttatcaatgg ccaattgacc gaactcttct gccgtctttc tttgtgcttc
     4681 agttacttcc tcttttgctt ttgtgccatc ttgttgcttc agctgatcga tttcttggtt
     4741 tttctcattg atggtttgat ttaacttgtc gatgttcgcc tgtgcctcag aggaattttt
     4801 tttgagcttc tcagacgcgt ttttagccac ttcttcaatt ttataggtat ttgctgcttg
     4861 ttcctctttc agctgccaaa acaacgcaat attgccgatt aaactaacgc taattgcagc
     4921 tgttaagacg atttttaacg ttttattttc catatttttt ctcctgatct attttttact
     4981 gctattcaaa aatggtaacg gatcgatcca tgtaccatca tcaatgaatg catgtccttg
     5041 tgcttgacgc caatccattc gagtaaatcc taaatgcaag tgtgctgtat ctcgtatccc
     5101 aattacttga ccaactttca cttgatcgcc tactttcact cgagaatttc ctgtactatt
     5161 tgcaaattct tgatacacca tattcaagcc atcgtaatta atcacgatca cacacgcgcc
     5221 caaacctgaa ataccaggat tgccaacgta cacgactttt ccgccatgaa ccgcatgaat
     5281 ctcacttcct ggatgatcaa ctgaaccaaa atctaatcca tcatggaagc cgttcggacg
     5341 gaattcaccg cctggattcg ttccaaataa ttgaccaccg ctgaaggaac ttttatctaa
     5401 actgctgcca gggaatggat tcgtccattc accagtaatt gtgccattgt tattgccacc
     5461 ttcaccccaa tgtttgatgg cgaattcatc atattcggtt aatttattag aagtgataat
     5521 tgacatacaa cctgtataat acggactatc ttcagtaatt cctgcagcgg cataaccacc
     5581 tcgtcctaag atcaataagc ttgcgccagg gcttttattt cctacacatg cattaaaatt
     5641 tgattgtgtg gctaccataa agccgtaata ggagaatgca gcttttgagt tgggaaactt
     5701 catgtaccag cccccttcaa tgccaccaat tcctcgtgca gttcctttag gaaacagaca
     5761 accatcaaac caagtaatcc caaaatagtt gagatcattt cttgcggacg ccgacgtacc
     5821 aaacgctgat tcataaccgt attgcgaaag aatcacactt ggtaataatt tatatttcac
     5881 tgctgcacct acggcatatt gcgcaatctc atcagaaaaa gggcctcttc ctttgatttc
     5941 tttataaatc ggtaagcctt ccgaatactc tcctgtaaaa gcttcgccac cactacttga
     6001 atcggaatcc gaattatcaa tcgaacccgc cgcaagtatt agaaaaaggg ttacgaacac
     6061 agtgatcaaa atagtaatca aacctgcaga agtaccaaac aaccacaaaa ataagcgtcg
     6121 ctttgcacga cgcttcaaat gttccataac tatatttgta tcattttcca tatagtcagt
     6181 actcctaact ttttaatttt aaacaacaaa aaagcgaaga aataataatt attcttcgct
     6241 gcttttaata aaataattta attttttcaa tacattcttc taaaatttcc tttggcacag
     6301 cttccacaaa ctgaaaacct ctactctcaa tgtcaatcat tcgatggtgt tccaaaagaa
     6361 tttgtccttt tattttttgc gtaccattca aatcaacatg tacaggaaaa ggcttcgctt
     6421 ttgttgttat cggtgcgaca ccaatcatat agttcaaata tttattaggt ttagataaaa
     6481 taatacatgg tcgttttccc cgttgttcat gtcctttggt aggatttaaa tcaatataaa
     6541 caagatctcc ttgcgataaa tcatgatagt tctttttcat taaatgatct ccctaccttg
     6601 catatttccc cattcatatt tgtcttcttt tcctaagggt tctccttcat aattggcgaa
     6661 caattcatct aaagtttctg gctttttatt tttaggagtt aaaacaattt ttccttctgt
     6721 taccaccatt tcgaatttta aattttcagt attagtttct cctagaactg acaattcttc
     6781 ttttccaaaa cgaatacctt gtgaatttcc ccattttttc atttcgattt cttttactaa
     6841 catggaatcc ctccttctct cacacattag tatatacttg tatacaccaa aatacaagaa
     6901 gttactttcc accatcaaac aatttcaatt gacgtttgtt cggactaaaa tgaacacgaa
     6961 tggtttcttt tgagccaaaa ttgatcacag cgtctccttg attttgttga ggaatggatt
     7021 ctagttctga gttcgtcatt gatttaccaa ctaatccttc tatatgacgt aaaatagatt
     7081 catccatacg cataaagatc ttcgtagaag aaaattcaaa gactttcttc agtttcgaga
     7141 agttatcaga agacatattt tctggcacca tttcttgtgg ggattgtgta gctaaaacag
     7201 tcattgcttc tactttacgc atttctcgtt gaaagtttgt gacataattt accgtttcaa
     7261 taaaatctgg attaatcgat actgataggt aagagtttga tattatctcc gtcttttccc
     7321 ccagtccaca ccgtacgtgc gactttcacc gcatacggcg ttccctcgtc tataaaagac
     7381 attctgtttt cccgtcctac aaatatttat taagatattt cttcgttacc aacttttaat
     7441 ctcaactcat tgagtttttc tttttgtttt ggatttaatt ttagatattt tagaagttcc
     7501 tcaatcgttt cactttgtgt agcgtgtatt acccgatgaa cctcctcagt aacaatcact
     7561 aggttttcat agctatcact tttgctttca tgataaggga taatatgatg acaatgcatt
     7621 tcagataaga taagctcctc acctgttact gcacatcttc ccttttgagc gatgaatttt
     7681 gaaattcgat tgtccgcaaa ttcaatcgtt gctctcttac taagcactgg attgctacac
     7741 aaccacgata tcttccattc ttctacggtt tgttgcttct tatgaatggc ttccctacct
     7801 tcttcggtgt atttattaat atttcttttt ttacacatcg gatgtctgtg ttgaatatac
     7861 cctactggta atattggaaa ccctcttata tatcgtattt gtcctgaccg taggtaaggc
     7921 ttgtatcctt cgtactttcc gtattgtcct tcttttgaga ttccttgttc tcgtagtcgt
     7981 atttcaaaac ttggtctgag ttctcttgca atttttcgca ttgctacact acaatgagtc
     8041 gcaattttga agtaattatg aatcccaata accatactgt tatagactcc aatatagata
     8101 aaggtatctt tgctattagg acttttttga attcgtttga tttgtttttt tagtttcact
     8161 ttaatcatgc tttgtgcttt tgtacctata tgcgtattaa caaccttttt gttacctttg
     8221 gtcactaatt tcatcgtaaa gcctaaaaat tcactttctt cttttgaaag atttgtgatt
     8281 tttgactttt cttcagatat tggtaacttt agacgctcct gtaaccacat tttagttgca
     8341 tagaaaactt ttttcgcttc attataattt ctacagaaaa ttttaaaatc atctgcgtat
     8401 ctcactaggt atatttcttt tagagttgta ctctttctga gcttgtgata gatattacct
     8461 ttatcccaag ctccattttt cctaaaatga tgttttagct ctttggcatc tttttcctcc
     8521 cactgatttg ctatccacca gtcaaattcg ttgagattga tgttcgcaag taatgggcta
     8581 agtattccgc cttgtggtgt tccttttgtt gggtatatca acttcccatt tggaagtact
     8641 acgggtgctc ttaacatttt cctaatgatt aagagaagct gtttgtctcg tatacccata
     8701 gaccagattt gtctcatgag tttagtatga ttgacttcgt cgaagaaacc tttgatatct
     8761 acatctacca catattccat gtgactttga ttcattcggg ccatacaatc tgctattgcg
     8821 tgttctgctg atctatttgg gcggaaacca tagctgtgat tattgaattt tgcttcacat
     8881 attggttcta atacttgtaa gatgcattgc tgcgcgattc tatcccatat tgatggaata
     8941 cccaacggtc ttaatttacc attatattta ggaatttcta cacgccttac tttccgaggg
     9001 ttataatgtg cgaatcgctt cctaacaatt tctacgaatt cattacggtt gagattttca
     9061 atatctttaa tcgttactcc atcttctcct cttgtcacac taccgtgatt tctcttgata
     9121 ttacgatatg ctaggacaat gttgttatca gaaatgataa tctgcattag attcttgaaa
     9181 ttatctccct tcaaactttt ggagtatatc ttatcgaaga tttccgtcat gccataatat
     9241 tctgcatgcc gcaacttcct atcttttgga cttgtttctt ttgtcatggt ttatcacgct
     9301 ccaattggct cgtgaccttt ttagtcatac gagaatccca tggttgaacg ggtttcatga
     9361 cttttacaga cgaatcgagc ctgttcctcc agttccatta cagaaccttc atcagtcgtg
     9421 actctacttt caccatcact aaagtaagtt atttgtcatt cacatatttc ttactaccac
     9481 ttcatcgagt atgcactctc catgttgatg gcttcccctg ttcccttata tctatccaca
     9541 tgccttaact tagatgctag cttttgcccc ctaacttctc ctacgcctgt aacgcagcat
     9601 ggtctttcat aacaacgact tttactcaac cacaagttag cacaatgagt gattttttgc
     9661 tcactgtctc tcccatttct aggagctatg cctttcggac agtacattcg ctagttcgtc
     9721 agtacgtttg tacatactca ccatattaag tttttttgga cacccgactt atcggataca
     9781 ccctttacct ctaaagaact ttcgtggtat tcactatttc ttttacctta cggtatcctt
     9841 cagccgactt caccgagctt cacacaacgt tggtctgcat aaactacttg ttgcatgtcg
     9901 gagtatcagt ggaagccctt tgtggcgtta cccactcatt tgacttctcg ttataagagt
     9961 tattacaata gtttttagac tacttgcaac ccacagcttt tcactgcaga acaggtcgca
    10021 ccattatggc actcatcaat catcgccaag aagcgtttga agtcttgttc ttccatttgt
    10081 ttttctttaa gtaaacgtcg ttgtcttctt ccttgaatca atgcctgatt ccaaatcaac
    10141 gtcatagcca tgtacatttg acattgttgt acccctcgat ctaagcctgt aatagagtca
    10201 atgtcaaaaa agaccaactg ttgttggtca aggtgtcgaa tcgtactgtg accatcaaaa
    10261 atcatgccat acgtctcaca taaggattct acgatggatt ccatttcttc ttttcgattc
    10321 tttttctctt gggaagcacc tggtaattcc agttgtccta aaaagtctaa aaattctttg
    10381 aacgtcggat aagcttctgg atctttccct gtgacttgcg tctccgtatt tgaacgcgaa
    10441 tttgaggaag taaagccgcg tggtactgtt ccatactctt cataaaaacg atttaagtag
    10501 gtcttaaatt ctgctttcat cgttcctttt agctgaggac tcactaactg tacctgatag
    10561 accactttgt ctaagtgtgt ttgataggac gctgcttcat tgatcttcaa atctttgact
    10621 gatccatcgt catataaact ggatgcttca tcatacgtcc gtgtggcaaa tatctctaaa
    10681 ggattgatca ttccgtcaga tccgtctaaa cgcaccataa taccgttata ttcctttaag
    10741 aaaggaataa agtcttttcc cttttcaaag ccccaaataa agtctcctgc agctaaatgg
    10801 gcttccgcga tcattttaag aacggtcgat ttaccagctc ccatcattcc tagaagcaag
    10861 gtattagtag acaaccgtac actattttta tacgtcgcat tccagacaaa tgggccgcct
    10921 gtcatggttt ccccatattg tgtccctcct ggatctttta gcgtttggtg gtggaacggg
    10981 aaacctccgc caatgtttaa cgatggtagt gattgccctt tgatatttcc gaaccattca
    11041 gcttgttcat cgaacatggt aaacatcgat tgccaatctc gtttggtttt atgcagataa
    11101 gtcaccgcac gataatccat ccctttgaga ttttcgatca gttcctgcgc tcgttcatct
    11161 acttcttgaa tcgtgtttcc gtaaacatgt actctcactt tgacttgctt agaaatttcc
    11221 ccattagaag tcaattttct agcatattcc gctagttcca ggtattcttc cgcgctttta
    11281 tttcggtcag tcgcttttcg ttcattatct gatcgatcat ttaattcatc taaggcacga
    11341 ttgatttgat caatcacttt ttgcttttca tctgtgctaa catcgagtgt ggccactgta
    11401 tgaggattgt tcatgagatt ggccaaccaa aaaagcgtag gattttttgt gtagtgataa
    11461 actgtcacac atcgtgaata accattacca tacgtcacat attcttcatc aaaatttaac
    11521 ccaccttgtg gcatcacttg ttttaaaaaa tctaaatcat accctttgga ttttaaaatt
    11581 tctatttccc gatttcctag ttgctttaat ggatcaagta ctttttgatc attttgtggt
    11641 gaaaaagctg ttttaattgc ttctaacatg tacagtcacc tcttcttgtt tgattcttta
    11701 cttggttaaa agattgccct tttcccaact tacccaggct taaagtgtga ggaaattttc
    11761 tagtggtctc ttccttcgga acatcttttt ctaaccatat caacggatat ttcattctct
    11821 cttctccatt cctactcatc attcatattg ttcattcgat acagaatctt gatcaattct
    11881 tttttgtaga gtggttttaa ccctaaaatg ccactagatc gcattaactg acggtttctg
    11941 gtttgtaaat ccttgattgt ctttccttca atgacgaaaa agaagcttaa gtcaggacgt
    12001 tcttgttctg ctcgtttcaa ttcctggatt tcctctacta atttcttttc atattttctt
    12061 aatcgctgta gatttcttcc taaggattgt tccatacgat tcttatctaa taactgccgc
    12121 ttttgtacga tttgttccgt taaatccgtc tctgtggtca aactaactaa cttaattgtt
    12181 ggcacatacg tacgcatgac ggtggtcaac tgattcattc gttctagttt ctcttgttca
    12241 tttaatcctt ccacgtctgt agaaactaca cgataataac gttgaaaatg atcttcttta
    12301 gtaactaaga ctccctcttc tgtacaggat ttataaggaa ataaatccaa gatagatcgt
    12361 tcttctggtt ttccaaactg aaaaatggat ttcttatcaa ctgaattttg tcctttgact
    12421 cgcatttatt catcctcctt atacgtacca tgataatttc ggttaggatc taacgcatga
    12481 taatggttct tgtccgtaaa aaaaatagac tcgataatcg caaaaatttg tcgttgacct
    12541 gggttattat agggtcgcca aacaatcact agagctatcg caatccataa gccataattc
    12601 aagagtgcaa aaaagagtgg taaatggaca aggttattga ctattttcgc ccaaaaaaag
    12661 caaccaccaa gcgttaatag atccaaaaag aaaaaggcaa aaaacttcca ttccccacta
    12721 aatcttggag gatttttcat gttaatatat ttatccataa atgatttctc cttttcatac
    12781 atagaaaaaa ggcctaactt gtaggccttt tatggtttga tatcctgttt gatatccctc
    12841 aatagttgtt ctttcaattc attgttcctg tttttagttt ctcttgtcac atttattttt
    12901 gttggtgcag taaatgccac atttgctggt ttgtaacctg tgtgaactct ttgaataggt
    12961 tctggtgcag cttctcttgt cacaacattt tgaacggacg cattccctgt ctccccactt
    13021 tcagataaat tgtaagaaac agttgtcaga tttccatttt caggcaaacg acttggtttc
    13081 atttgaacat tacctgcagt tgattggtga acaaagcttt gatcattctc cttgaattga
    13141 ttcaaacgac tatctttcat tggtaaattt tgtgccttag gtaaaccaat tttcgttgat
    13201 ttttcagttc caacttcgac cccattatca tttggcagat cgatggaatc atcatccgtt
    13261 tgtacttctt gatttcgttt tggtaaatct gaaatgcgct tttcattttc agaattaatg
    13321 ttagatttat aacgatattg ttctggatct gtttttggta attgaccatt ttgtccttct
    13381 tctgaatttt cgttagaatg caactcatct tggtgatctt tggctgaagg aatcgctgca
    13441 ccttcttgat tttctttttc ggttgttgta ttagctttta ctgctgcatt gacttgtcct
    13501 tcaccatacg catcttttag tccttctttg acttctgaag ctttttcttt ttgtttttct
    13561 agtgcttcgg atggattctt cacagcttca gccaattctt tagctctcgt gccgtctaat
    13621 ttctcttcta aggtgtcttt ggctttttct gtaagatctt cagctgttcc ttttaaccca
    13681 cgttcattga cgtaaccaag accttctgcg gctttagaaa caccattttt cgctttcccg
    13741 aacatttcgg aaactgtttt cttactatta ggtacagttg tagctttatc gtctgattgt
    13801 tcttccccgt tatcaggggt aacatctttt cgatggccaa atccattttt acgaatatca
    13861 tttaaatggc ttaaaccact cttaccagca cttgcaaggt ttttagctcc gctacccaca
    13921 tttttcgcaa tatttcctaa ggcaaacatg ctaagaagag aattgctgcc gtttttcaga
    13981 ctcgtatcca caccgaaaat tttcgtaact gattgagagc ctttgaataa cgcaatggtt
    14041 agtacaatta aagcaacgac atacaaaaat ccattgattt ttcctgccgc atgcaaatca
    14101 ccgatacctg aaagcattaa tgtatagatt cgtaattcta aaatcgtaaa cgccaataac
    14161 aagaaagact gagaaatatc ttctatagca cttttcatcc gttgtcctgt ctcaaagtcc
    14221 gttgacgcgg caatcactaa atagaatttt ttaaatacca gctcaataat gcaagtaacg
    14281 atcgtaaata ggataaataa ataggctacc gacaaagaag attcaccagc taaaataatc
    14341 cctggactac taatgtatcg acgataccct gaagtataaa catatttggc taagccttca
    14401 tcactgattt gaacaggaac ttcttttcca ttatcatcca gttccagtcg gtattgtaac
    14461 gctcttaacc gatccttttg taaatctgat aaactactct tatctttatc cgtcatttct
    14521 ttagcttgtt taggagtaat aatcccattg atattcgctg ttttaaaatt tttcgctgtt
    14581 aaagcatttc ttttatctgt tggtagggat tctactttat tcgggtcagt tgctagtaat
    14641 gtacttacat cgatcacatt attttgaata atttgaaaag caggtgaacc cgttttatct
    14701 gctttatttg ctgcagtgac gtctccataa aagtttttac tgatatttaa cccttcatcg
    14761 attaaagaac taccaccaaa aatcaagatc atggccataa ataaattaac caagatattt
    14821 ttgacttttg gtggatgttt actcataaaa aatttcaagc ctaaataaat taatgtgacg
    14881 acacaaaaaa gagccaatac cttactaatc aaactttgaa acagttcatt aacaccagcg
    14941 gcgttcaata agtcttttaa atcaaagctt tgataaattg ttttttctag taaagtattg
    15001 agattatata accctttgat caacccccat aaaaaagtat tacaaatcca actgaacaca
    15061 tcttgatact gcagataatt accataatct tgtaagactt tcgttacggt tttgggatct
    15121 gcatcccctt caccaaataa acctgaccac cagtcggcta tgccagaaaa aattcctcca
    15181 atgatcattc tctcaccgcc ttatcagctt ccaataacaa ccgtttccca attatttcag
    15241 aaatttcttc atttttgatc aacttttcaa catattcttg aagtcgatct gctgcttgtt
    15301 cttcgtaaaa ttgaattaac aatttttttg ccgtctcatt ttcaagagat tgaacaattt
    15361 tataaaacat attttcttcg attttttctt ctaattgttc atgcagtcca tgatagagat
    15421 ctctagcatc ttcatctggt aagaaataat cataatcaat ctgtaatgcc tttaaatcga
    15481 gatctgcgtg agtacaataa atatcgatat catgaatggt tcgtcctaat gtatcgaaat
    15541 cttcaagata ctgatacgca taaggactat ttgttttttc agaattaaaa ataggatagg
    15601 gtcttatctt gtttccttta agatcttcac gctttagcgg tgcaagaaca atggtctctc
    15661 cttttaaaag actcctaatt cgttcgggag gcaaaatacg atctgaatca acattacgat
    15721 tgatatgtgt gttcattccc aaagcattga tcgtactatt ttcttgttcc actgttttgt
    15781 tgccgacgtt ttgagaaaat tcttcaatcg tatctgtatt tgaagacata atataaatat
    15841 gtgtttgaca attttctttg atgatatctg cattatcttc accatatttc gcacgtagtt
    15901 gtttatatga ttgaataaac agatcccata acatattacg acctgccgta accgtcatga
    15961 ttccttccat gttgtccatc ggtggcatat ttccaaattc atctaaaatg aaatgaattc
    16021 tacggtgaca tttatcacct ttggtcttaa cacactgtgt agataattct ttatatagct
    16081 gactaagaaa aatgctaaca atgatatggt tagatggatc ataatcaggt gttaacataa
    16141 aaatagccat tggtttatcg gtataatgca tccgaataga ctctactagt aagttacttt
    16201 tactttcatc cagtgtgaga actgcttctt tttcccattc tggcaaatga gtttctcctc
    16261 gtttttttcg atagacgatg tttccctttt catctttcaa tactttttga gagaactcat
    16321 accaggccac atattctttt ccttggtgat catattttaa gcaaacttga tccccttgct
    16381 ctaaatgaca gtcaaaatta atttcacaaa attctttata acctactttg acacgttctt
    16441 catgaaattt ttttccatca tgatagaact ctacaccaat acgttgattt aatagacttt
    16501 cgtgtaattt caaatctaaa tatttgggaa aaccaaccga tttataatca atggttgatt
    16561 gagaagtcat tttcgcaatt ttcgttaggg taaatgggtt caatcctgta attgccgtcg
    16621 ataaaatatt cccttttgct ttatcagatc caattttcgt tgctccatat tgtttttttg
    16681 ctagatttcc aggtggcaac tgattcataa actcatctaa agcattgagt tgcttaaatt
    16741 cattagtagg atcttgataa ttgaatccac caatttctga aagtagttca acgagattat
    16801 ttggtgttaa tttggtgtaa tcccctgttt gatccgcttg ttctaacaac gcaaaaataa
    16861 gcgcattgac agctttttgc gcattttcgt tgaaatattt attgtcagaa gcattcgcgt
    16921 cataataaat cgtatttgta aatgtattcg ctagttgcgt agcggtatcc acgtctcctc
    16981 gctgccaata tttcacaatt aaagccaaag gattatcact catggactgt aatggttcag
    17041 ttagattata agcataaaca tcatagcctc tggcttctaa ttcgtctttg ctggctacat
    17101 acaactcatt tttaggatca ttgacgacca atgaactttg tttttctgca cgagataaat
    17161 tatcaatagt cgctaatact tcaatctgcc ctttccctga tcgagaagtg ccaataatac
    17221 aggtattgac ggtatccgtt tggatatacc agtacttttt atagtgagtg ataggaattc
    17281 caccatatcc aggaaaagaa cctttcgtat ccggtatctg aggatagatc tgttggattt
    17341 cttttagcgt cattaaccga ttatcccctt tttgtccatc ggctaacgga cgaaaccaca
    17401 tatccatttt caagcccata agtacagctg gaacaacaca aatcgtagaa acaactaagc
    17461 gtgtacctaa actcgttgct ggggtcaaag gattccaaaa accagctgaa tcaaaatgaa
    17521 agacctgtgg catttcctgt aattgaaaca gattcactcc ttgtaagctt ccttgaatga
    17581 tttcaaatcg actgaaagct tcaggtattc ttgtcactaa ataatctaaa gggagataga
    17641 ctaaaccgct atacattagt ttttgtttga ctcgcttatc atgcttacct ggtaatcttt
    17701 cacctgctgg tacatagcga taaaaatgtg atagatattt ctttttttct aaattaaaag
    17761 gcttgtcttt tcgtttcgtt gtttctcgaa tccgcattat ttcacctcat ttgattctga
    17821 actttcggaa gagtttgatt gtacagttga atcgcttgat ccttctttgg tcttactaga
    17881 atcactctct ttactgtttt ccatacttga ctctgttgtt gactgattgg tacgattcaa
    17941 caattcttct aattgttgtt catactcttt tcttgcttgt tcctgggctt cttccttgat
    18001 tgacgccgtt gtggctacag aatcagcctg tgaagattga ttgatatgat taaaagcaaa
    18061 tactgtccct ccacctagaa gcaagcttaa acttgcaata catcccacca tccatccgtt
    18121 aaaggaacgc tgtggtgaag gagttagatt tgtttggttg ttcattggcg tttgaacagc
    18181 agctactgaa ggcattggtt gaagttgtgc tagataattt aattgtgctt gtcgcaatgt
    18241 atccatttgt tcttgacgtt ccttacgagc agctgcttct ttttcctttt cttgctctaa
    18301 tcgttgtttt tctatcgcca ttttttgttc cgcaagatcc tgttcccgtt ttcttatttc
    18361 taattgttca cgctcttttt ctttttgccg ttgtttttct tcttgttctt tcaattcttg
    18421 ttgttgaatt tcatactctt ttttccattt tggaatttgt acttgtaatt cttgctggat
    18481 ctgttcgtgt ttttccataa gaacttcagt tgttgtttga tatagcttat tcagttgctc
    18541 atttaattct ttgctaatct ttagcttatc tgtacgcaaa ccgcggtaga actcttcttt
    18601 tcgttcttta atacgttctt ttaacaagtt ttctttttct ttcgataacg cttcttgatt
    18661 cgtcttagta aaattgttga tcttttctac acacaaggaa taagcattgt cgcgtttctt
    18721 ttcgtaccgc tctttgattt tttgtacttc ttcttcaatc acttgctgtt cttctttatt
    18781 tagttcctct tttgctttct cccaattgga agactgttcc gccgcatact cattaatttc
    18841 ttgatctgtt tcctgttggc gttcttcaaa ggtggctaat agatcttctt gtgcaagctc
    18901 atgccaattt gttgattgga cagactctaa ctgttctaaa agtgtgcctt tcccctgtga
    18961 aattaattct tctttactct ctaaaaaagc ttgataggtt tggggtaatt gatatttcga
    19021 ataaagctca tttaaaaata attgattttt ttctttcatc cattcatgct ctggcggata
    19081 ttgatccaaa atataatcaa atacagttgc cttttgtatc tcagtttcgt ttttctctac
    19141 ctcttcactg ataagtggtt caatatattc ctgagaatcc ccttcagtcc atgttggctc
    19201 ttcattcgct tcaactggat caatctctac ttcggtcgtc tcttccattg tttcttcaaa
    19261 gggatctcca tccaattgat ccattacctc gttctcttcc tcaaatatcg atttctgagt
    19321 cggaatatca gcttgttcgt caacattgtc tgtctctttt tcttttgatt catcctttgg
    19381 aaagattggg cgttcccatg gaacttcttc gtttacttcg acttcactca cttgattcac
    19441 ttttctttgg tcgctctcta gctcttcttc taacacttgt agcaattttt catttcgata
    19501 agactcttcc acactcatgg tttcatccgt ttccttaaac acatcaaggc ggatcaaatc
    19561 catcaatgta ggatagaaac cttctgaaat ttgataatcc agcggtccaa aaacgtattc
    19621 tgtaccagct ttctctattt ttccttctgt gtcatgtaac gtgagagtca acttaacaga
    19681 agattgactt tcttgttcca gtactttccc taacacatgt agttgttctt ccgtttcaat
    19741 gaccgaatat ttcattgttt ttggtaaaaa agaagtccgc tttcctactt ctttcgttgc
    19801 tttttttatt aactctttat cttttttggt attgcctgca atcgtaaagt acatgagtat
    19861 tctcctttca taaaaaaagg agtaaaaagt acatagctac tttttactcc ttcattttgt
    19921 ttttataatc cctgtggggt ttgtccaaac attccaaata acgaaaccac aatagaggca
    19981 gcagctaaaa taattcctac ccccatcaat acataaccga cgtgcatctt cccgttttca
    20041 gcacctcttc gtccaaataa gaagaaaagc cccccaataa caatggaagc aacggccgca
    20101 acaccagcaa aaatcatcag attatttttc aaactagttg cggcagtttg taatgctcta
    20161 aaaatatcaa ccgcagctac tactttcggt tgtgttaaaa aattcattgt aattgattca
    20221 aacataagat tgttcgctcc ttttaatgtt taataatttc atcgaaaaca agtttttcac
    20281 gttcacgttg atagatatat ccttctaagt ccttatcttt tccccgtaat aggtcaaatt
    20341 gtgctttttc aagaacttct tgtttccact gtgtttcaga aacaccgcga cgttctaaga
    20401 ttttagacgc ggctttttca aatggcgttt tagcttttgc cattgtgatt cctcctttag
    20461 gataaataaa aaagactagc catcactcgc tagtcttttg taatttgtat ttatttgatt
    20521 gtgatccatc atgaattaat ttctctttcg ttcgtttttc atccttacta gatccaagaa
    20581 aaaccatttt tttacgccat ctttctcgta aggaacaacg ataaataaag aatctggttt
    20641 attgataaag tcgctaatac ctgcagaatc tactattttg tatctttcta aacctttata
    20701 gtctttttgc ctcttgactg ttatttgtac aggatctgga tgccatacac tttcagaata
    20761 ttcccatgta gtatcgtcta taattttcaa attattaaca agaccaccta cttcttcttt
    20821 ccaaacagtt cctggttgca ataaactatt atcttcttta gcttccgatt tcttattact
    20881 tgaacatcca cttaaaataa taaaaaatat tgcaaacata gagacaaacc atagttgctt
    20941 tttcttcatt ttattccttc tttcgttgtt ttattttagt atatcaaata acgaaagtag
    21001 caacaaacct cattttgata cacggggaac ggatactcat aatttcccac ctttctatag
    21061 aaacttttcc tcttgatatt gtgatttatt acaaaaaaga gctataataa agaaaaaaat
    21121 caagagggag cattgcaatg tacgcagata agaatgaaat cgaatggtta ttatttgaaa
    21181 gtggaatctc cgttaaagat atatatatgg aatcaggtgt cgcgcgcact acagttatcg
    21241 acttgattaa taaaacctcc tcaatcgata aaatgcgttt tgataatgca gtcaaactca
    21301 ctaaaatggc gaaaatgcga aaaaacttat atgaaaacaa ataataagaa accaatgctt
    21361 attcaaaagt attggtttct tattgtattt taatcggtat accgtcattt actcttgact
    21421 ttgtcggtat accgacatat aattaacttg taaaaacatt taatctattt aggagttgaa
    21481 aacatgaaat tattaaaccc aaaaactgtt atagctggaa catttgctgt cacactggct
    21541 gccacacttt ctcttgctgg atctgttgac gctgcttcaa ctagcgtcat gtatcgcctt
    21601 tataatccaa ataaccatga acatttctac acctctagca caaaagaacg tgatcactta
    21661 gtgaaaatta agtggggtaa ttatgaaggc cccgcttggg aagcaccgac tagcggtggt
    21721 catcttgtct atcgtttata caataaaggc cttcgtgatc accattacac agctagttgg
    21781 gatgaagtga aatggttgac taaaaattac ggctggactt atgaagggcc tgcctggcgt
    21841 tctgccgaaa aaaataataa accgatctac cgccttttcc ttcctggtgt aaaatctggc
    21901 tcacatcact acactgcaag ttggaaagaa gtacaatggt taaccaaaga ctatggctgg
    21961 aaatacgaag gtatcggttg gtatggcgct gacacctcaa aacccaaagt gaataaatcg
    22021 gctttagaag ctctttatac gagcgttaag ggaacgaaaa aaggtgacta taccgacaat
    22081 acctggaacg cattccaaac cgctttaaac aatgctaaga aagtgttaaa tgactcaaaa
    22141 gcgactcaaa aacaagtgga tagtgccaaa aatacattag attctgctta caaaggatta
    22201 aagaaaaaac cagcacctac tgtgaataaa gctgaattac aagctttata caaccgatta
    22261 aaatcaactg ctaaaggaga ttttacagaa gaatcttgga ataattttca gactgcttta
    22321 tctaatgcta aaaaaatact agacgattct aaggcttctc aagcccaagt aaataatgct
    22381 aaaaatagtc ttgattctgc atttaaaggg ttacaacaca aacctaaacc ttcagaatat
    22441 gcggtaaccg tccgtcatat gaatcgacaa actggaacgc tgctaaaaga agaacatgtc
    22501 tcagtaaaag ctggtacaag ttacactgca aaagctaaaa ccgacttgac taactttgaa
    22561 gcaccacagt accaagtaaa tgggaatgaa acacaaacac aaacgattaa cacaaatacc
    22621 acatttactt tttactatga tgaagtcttt ctagttgaag tgaaagctcg tccaaataaa
    22681 gtgtcggata ctgatccgaa tatccaagaa ctctataatt atagtaagag ttatcctgtc
    22741 gtttatggac aaagcatcac aattgaagca cctacgttca ctaattttgt tcttgaccct
    22801 agagaaagct ctagcaacac tgtgacttta aacaacgtta caagcaatca aggaatcaat
    22861 tttgcctata ctcatcaata caacgtcact gtgaaccata taaacgttga cacaaacgct
    22921 gttttatcta ctgagacaca aacggtttac gagggagata atttctctac ttcttggaaa
    22981 aatatgaccg atcaaaacta cttcttgtgt agaaatgacg attcatctgt taccgttgat
    23041 aaaaatggac aacgtataat taatcatgtt gaccaaaatc gaacgatcac tttcaaatac
    23101 aaaaatatct cattaaatga tttaaacaac tatgtgcgtc aaaaagaact tgcttggtta
    23161 aatagctacc gtcaacaaaa tggcgttgct ccaatgcaat ttaacgacgt cgtacaaaaa
    23221 gctgccgata tacgtgcaaa agaattacaa gtaagcttca gtcattatcg acctggcggc
    23281 ggtactttcc aagatctctt agaaagttta ggctgctacg gcgctaaagg agaaaatatc
    23341 aatagtttct acctttacgt tgacgagatc ctttcagata cgggagcagt gaatgccatg
    23401 attatgtgga aagatagcgc tgctcataac gcaaacttac tttacaacaa tcaaagcatc
    23461 gttggtttat cccacgctta tgaagtttct gcagacggat cactttcttc agataatgta
    23521 tttattagtg ccaacccaat tttcaaatag aaaaacaagg ctgattttca gccttgtttt
    23581 tttgcttaat taaacttaag aaccacataa ctaaaagtag ctaacacaag taatagtaag
    23641 ctgaccacta aagttgttac taaatgtgta gaacctgttt taggaacaat aacttgtggc
    23701 aagttaggat caaccccaat tgtcacaaca tttgttttat tagaaacatt atctactgct
    23761 actacggctt ggttatcaat atttttttct gattgtagtg gggttttagc tgtaaatatc
    23821 agttggattt ctcgtccatc taaaagattg ttacttaaga agttagcgga agcttcccac
    23881 ttcagcgttc ttgaattgct atcataggtt agtgtgcctt cagaagtaat atcttttcct
    23941 ttactcttat ctattacttg cgcagaacct tctttatatg tcaaacgagc ttcaattgga
    24001 tcttgaatac taaattgttt gtagtgatct gacgcagcgt ctacgccttt tgtgtgccat
    24061 ttttgtgtga ctagatattg tacttcagat cctgcgttta aaggagctgt cggtgtaccc
    24121 ttcttaccgt catttcgtcc gtttacatct tttccatttt gttgcacttc tttcactgga
    24181 attttaggga aataaacggt aatgatattt gtatcctttt ctactttatt aatgatcgac
    24241 gttccctgat ttttggctac actattttga ttggccaatt cattgacttt tacattgatc
    24301 actaattcgt aagtttcgcc gttatacttc attcgatttt ttaatgtatc agctttcgct
    24361 gcatatttta cctggtgttt tgttttgtca taggtcactt caccagatac atcgaatttt
    24421 ttatttgatc cacattctac gtgtgcatcg acgtaattta cttcttttgg taaattatca
    24481 attaactcaa atcgatcgta aaccgctagt aaatccactc ctaaacgatt cactttttgt
    24541 ttgattcgat attgaatcgt gtctcctact tgcaacattt tatcgttcac gttatttcca
    24601 tttttatcaa cccctgtttt ttcaggttga tcaggaacaa cactaaagat tgtcgcagaa
    24661 gataaagtaa accaggtttg aacattagaa gaaccaatca caaacgtcgg atttgcttca
    24721 gatattttaa aggacaccgt accttgacca aacttcggat ctcctaattt atcatgcaaa
    24781 ttattgaaat cattatccgc ttgtgtcagc ttagagcttc ctcctgggtg gccacctacc
    24841 acgttcagat tgttgtagaa agatttaaat tctgaaagaa ccgtatcttt tctgacatac
    24901 caatctgtcg tattcatttt gtcatagtgg gcatattctc ctttaagatc cgtgcttttt
    24961 cctcctgcag ggttcaaaga gttaaaactg ataaatgtat ctcctgaaaa ttgaacgggg
    25021 ctaccgtcct catatgtggc tactagctgc acattcattt gtgttgcatg gtaaacaatt
    25081 cctgagaaaa gattgtctga gatccaaagt actgttcttt ttttagattt actcattgca
    25141 ccattcgtat ttttatagcc atcgtctcga aaatgagtct ttgtatagtt gttatctaga
    25201 atattccatg gcaacgtatc tgaatataag tggatattag atagaactag cttgatattg
    25261 acttttttcc cgttatacgt accgattttt ccgtagtcaa tattgacttt ggtgttggtt
    25321 gttacgcctt ctaaaacaag gtttgtttca ccgtattctt tttggaaaga aggatagcgt
    25381 atatttttgt ttccctccac taatcctacg tagtcgtctg taaaaccacc tttgttggca
    25441 tcaacatgat atttgtaaac tttggacgct ccgcttacag taatatttgt ttttgcatta
    25501 ggtacatctt ttggaataat aaaattattc tttacgttct ggattggatt tgacggatcg
    25561 acaataactt tttcagcaaa gacattggct gtttttaacc caaacaatgt acctaacatc
    25621 atcattatgg gaaaaacata ttttaaccgt cttgtccacg agtgtttaaa attcatccct
    25681 tcatcccctc ttttttataa atgactagtg aaattccgac aacaacgatc aaattgatcg
    25741 tagtaaatag tggattccta atgagctttt ccataatatc tagcggcatg gaagcagctg
    25801 gtatgtttaa aatcctcatt aggtatggtt ggtaacttgg taaaatagat aattgagaaa
    25861 ataataaacc gcagtacaaa aagactgcgg ttaaacaaga aagattgaat aataataatt
    25921 tgatcagttt catcttattt cttccttaat ctctagaaaa caagtgagtc acaaaatccc
    25981 aacaattttc aaagaaattt ttctttacgc cagtagaaac gatttttttc acttcttctt
    26041 taggttgcga agcttctgat ttaggataga tccacacatc ctttaagaat tcaccgtttt
    26101 tatcagaaac aggtgttaat agcaaaatat cctcgctatt agaaatgttt tcaggacttg
    26161 tttcattgac aatataataa gcttgtttct gatttgcctg tacttctacc gagaattcaa
    26221 agacacctgt ttgttgacca attgtctttg tttctccatt agaaactagc ttcaattgat
    26281 cactggataa ttttttcact cgatctttca attgggattc aatttggtca gtagtgagat
    26341 tcttaacatc catcttttgt aaaatatccg atacatcata aacagaaaac ttcgcgccat
    26401 ttactccttc ttgtttttca agcttttgcc cctggttgtc taattgttcg gaagaaataa
    26461 tcttttgaac gtgaatggtc tgtttgacta tttgatcgtc ttcagcaatt ttaggttctt
    26521 ctgaagaatc aactgttgac gaatcgacgg tttcatcgct gctttgaatc gtgctgctat
    26581 ctgacgtttg gattgtttgt tctgtgctat ccgtgctttc ttgactgtct gcaaacgtca
    26641 actgtgtgta ggcagtgaaa gaaagaagag cggctgcaca aacaaatagc cattttctat
    26701 ttctcatgtt ggttaccctc ctttattttt tctttttata aacaaatagc gaaccaatga
    26761 tcatgactga aagaaaaaca gtgcttccta aaagccattt ccaagataat ttttgtttct
    26821 tatttttttc atcttttaat tctgattttg taaaggttac tcgtttccca tacactagga
    26881 aacggtgtgt gttgattccc ttaggcgtac atgtcattag cgttactcga tcttcattgg
    26941 gatttggttc taagtaagtg acaaagtttc tttgtagttc gtctttttgc acttcctcaa
    27001 tccgatcgat ttgataagcg tgctgctcac catcaacttt gatataaaac ttttctcctt
    27061 tttttactga aggtaaatca tcaaaaagat tatctttata aagtccacta tgacctgcga
    27121 ttaaaggatt tttaccgttt cctcctgtaa tatcaccagt tccttccgta atacctacac
    27181 cattttctaa aattttgtcg gacgttccgt cataaattgg tagttttaaa gaaatacttg
    27241 gaattgtaag aataccaacg ggatcgccta actcaatctt agcaacatcc aaagaagaat
    27301 ccgtttctgc tgccgttcct gttcgatcga ttgcgacaat ctgctgattt tgttggtatt
    27361 tcgccacgct cgcttcagca ggcatcaaaa gtaagacacc tgcagctgaa agtaaccaga
    27421 aagcaatgat ccatttaccc atgattcgct ttttcttagg cttcctgata atcattggct
    27481 ttgatccttc taggctttct tcttaaagta aacaactgca gaaagtgccc ctgctcctag
    27541 caacaagcaa gagatcaata catacgtttg gaaaccgtta ctaccagtca ttggtacgga
    27601 agctttttga ttgtttttga tcgtgattcg gttttcttct ttgtatgaat ctttgtctac
    27661 tttgaaagag atctctttcg ttaatttttg atagttttca ggcgcttttg tttcttccag
    27721 tgaatattca ccttcagtta aaccggtgat ttcaaagtga ccttctttat ctgattttag
    27781 gatcgctgca tttgctacat cgtctaccca tactgcttct ttgacaccgt ctttatccgt
    27841 atcaactaat ccaccaaaat attttccttc tgcgttttta gcagcaaatt ctgcgccttc
    27901 aagcggtgtt gctttgattc ctgttccaaa tagtccgcta tcgactttta agaattttgc
    27961 accataagtc acaacgtcag ctttcgtttt ttcgtggtgt tcttcctgat taaatggatt
    28021 gttgttataa acgaaatcca cgctatttgt taatggattg gcaacagctg cgctgtcatt
    28081 tacatacatg ttataagtca tttgaagatc ttgtccgtgt aaacttgcta cagtatcact
    28141 tacttggcca ttattcacga taaaatcgat cacaaagcca ttttctgaat tttggatttt
    28201 ataatctgta ttttctttta ataccacaga tccatcttta tttgttactt tgatatttgc
    28261 tttagcagcg acaaaagaaa gtccttgtcc tgctgtatcc ccccatttaa atacactgta
    28321 aggagaagtt tcgcttgtca caccgtttac tgtgataccg tctgcgccac ctgcgatatc
    28381 tgtagggata tgaattgtac cttgataatt cactgcgtca ccaatttgga aagaatgttc
    28441 ttgtcctgtt ccatttgtca ctgttttatc gatcactggt gctttatagt tcacatatga
    28501 agcttttaaa tcgcttgtca caccgtcatt cgtaatttca aaggttaatt tattgtgcgc
    28561 atcgttgcga gcgtctgcgc ctaataaata ttgatctgct gttggttctt tatctgatcc
    28621 cactacaact tctgaaggaa cttccacaaa atagtatttt cctaacgtca aatcagtcgc
    28681 agtcagcttt ccttgatcat ccgatacaag atctcctaat tttgttcctt ttccaggttc
    28741 gcccttgtat aactcaaatt ttgccccttt taaaggtgtt tgtttgcttt gagctgtgcc
    28801 atcgtcacca aatttcgtta attcaaaact caacttagaa acaatatttt taggatataa
    28861 gtgaatgtct tttaaaaaac ctgaacctgt attatctgtc attggcgcaa tcacaaccat
    28921 tggttttgct ttttgtgtga ctaatccagc agcagattta ctttcataca ccacatatac
    28981 attccctttg gaattgacat aggctggttg atccgcaaaa gtgatctcac cattttcatc
    29041 tacagctgaa gtaattttat tggttgcatt cttgacgtac tcactgttag atcccttgtc
    29101 ttctacatct ttaacgattt cgtccacttt cgcgttggtt agatcagaat cttcagtcgg
    29161 taatacttga tccgtaatat ttcctaacgt aaactcaaca tcaccataat ctgccttgtt
    29221 gtatttcgtt acacctgtag gatattgatc gtgcgtataa ccatcgtttt tgatcccgtc
    29281 aatatcaaca ttcaattgcg ttccttgatc gtacattaat ttatgaaccg tgatattgac
    29341 cttctctggt ggcgtttttt gcgtgacatc ttctgcatgt gcttgatacc cctgaaagca
    29401 gcttgcaatg attcctaaca ttccaacaag cattccagct ttttttacat accgatttat
    29461 tgccattttt tactctcctc atttcatatt tcaacgactc gcgttgatta aaaccttgtt
    29521 attgtagctc tccctgtacc acgtatcttt cttttccttg tgacgtataa acacaagtaa
    29581 acaacgttaa taagggtttc tctcctttct ttggcaaaga caagaaagag ttctcttcag
    29641 gaacgccatc gattaattta aagtactcag gtacgataaa tttacgcgtt accttgtaac
    29701 gatagacgcc atcgttacta actaagtcca tcacctcccc taatgacaac ttttgaatat
    29761 cagaaaataa aacacctggc atttccatgt tatgacctgc taggacatag tttcctttac
    29821 ccatttcggc atcttcataa aaataagtag ccgctccaag actcaaagtg tattgatttg
    29881 cccctttaaa aattggcaat gctaaaccaa gtgcaggaat ctgaatttgt gcaaatactt
    29941 gtttattgac gcccatttcg attgcttctt tttgtatgga caacatttgc cccaaagaca
    30001 tatcattatt gacgttcgtt gcatcctcct tataactctt tgcgtgtgca ttgatttgat
    30061 ctacgggcat ctttttttct atattttgct taaacacctc tccttcatgt ttttggtagc
    30121 taacatagcc aaacacactg caaacgacga ttaaagcgac tcctaaaaaa ccaaataacc
    30181 attttttcat ccatctgcct cctttcagac aaaacaaaaa acactttttc acaacgaaaa
    30241 agtgttttct cttattacta ttctatttca tttatctggt ggttttatat tggtgtgtag
    30301 ccattgctaa cattaagaat acaattggca cccaactgat tgtcgttaat gtttcttcca
    30361 tgaacgattg acacatgatc gctaaaaaag cccacaagaa tgttagttgt ccatttgtca
    30421 gctcacgttt tcttacgagc aaccataaga aactaagcaa aagaataatc gatagaacaa
    30481 aaccaatcaa tccatagtga ttgacgagtg cgtgatacgt attatgatta cctagaatct
    30541 gatttcctct atggaaaaag aaaggtttca tccctacaaa tatttgtcct gttttttcgc
    30601 ctaatgtttc atagaatttg cgccaaattt cttctcgtcc tgtaaataag tgaactttgt
    30661 ctgacattgc agcaaaataa gaaatcggta aagctagaac cgaaactgct gcatacatcc
    30721 aaaaggacaa gtgtgtattt cgaaaaacct tcttaggtaa aatttctaag ataatgaaga
    30781 taacaagcgc atataatgct gctttcgatt ggcaaaccca aatcgttgcc ccagcaagaa
    30841 tcaacgctgg taatgatagc aatcgcatgt accattttcc aaatgaacta gcaaatcctg
    30901 tgataagaag ggctaatgtc attaaagaac tacctatcgt atttgtattg atccaaatat
    30961 ctgataattt gttcgatgga ttccaaatac tttgtccttc tggtactggt atctcagtaa
    31021 atactcgata aaccgttacg agtaaaaaga catagaatac tacaagatac aagctacgat
    31081 caattacagt aaatttcgtc cattttacaa tcaaaataat agcaataatt gtcggaacga
    31141 tcagatcacc atagaaagta tctcctcggt agcttctaaa aaaagaaaag aaaaggtaaa
    31201 aaagaatgcc tgctgcaatg aagaatagat cgtttctctt taccttgtac agattcaaaa
    31261 gaaacaaaca gcctacgaaa aacgtcacta actgtgcagc attatcataa ataaataata
    31321 tcgtaggagg atttaacccc accgtccata ttaaatatag aatggatacc aacagtaaca
    31381 gaaaaatggc actcatttta ttgatcaatt tttctacaag ttcttgaatg tgactcactt
    31441 cccttattta ttgatttttt ctacattgac taattcacac tggatcacat cgcacttttt
    31501 catattattt gcttgaaagg ctaacagtgt gagtagtggt tttttacctg cttttggcaa
    31561 ggataaaaag gaattgtttt ccttagattg aactacctgg ctgtaatcag aaatttttcc
    31621 tcttttggag acttcatact gatacttgta atgagcatcg attagatcaa tttcttctcc
    31681 ttcagataaa tcagtcatat cggataataa aacgcttggt gtatctacgt tataaccagc
    31741 aagaatataa tttccatttc ccatttcagt atctagataa taagtagaag ctactcctaa
    31801 actaagcgtg tcgctattcg ctccttgaaa aacagggaga ataaatcctg tagaagaaaa
    31861 aacaagtttt ccagcaattt cacctttgag atccttttct aatgcctgtt tttgattagt
    31921 aatcagttgt ctcttagaaa tatccgttaa actggttaaa ttatcttgat aggttttagc
    31981 ctgttcctga atatggattg tttttgttac accatctact ttttcagcga ttttgtcctt
    32041 ttgataacct tgataagcaa aataactaaa aagaagaaaa acaatgatta cgcccacgta
    32101 aggattaaat aaagttttat tgtttttatt catctatcta ccttctatat atgaattaat
    32161 ttacctacat ctaaccaaga taaaccatgt taaattttac tattcttttt attcaataac
    32221 aatgccacat cataattgat cattttcata tcataatcgt caagaaactt ctaataatag
    32281 ttggcaattg gaacctgttt ttacataagt tcgcctcaac tgttcaaagt ttttcattga
    32341 acctaacaaa caagctttct cttttcctgt tatcttcata tcattgatat attgcatgac
    32401 agataatctt ttaaggtgtt tttgtttcat catttttagc aacttttctt ccatttcttt
    32461 cgacactttt tttctgtttt cgatttcttc ataattcatg agttttccta cctttcttgt
    32521 tttttgcaaa aataaaaaag cctatgatca catacgctct acacttcagt cttgggttat
    32581 ctttttcttt cgtttaatta ctggccgtgc tggtttcaaa tccttgattc cattatgggt
    32641 tctccaataa tcgaattctt ttcgtaatct ttctttttcc atatctgttc ctttagacag
    32701 ggatttgaat acatctttag gtgaataacc acgagctaac agctgtacat accttccttt
    32761 tgtcaaagta tccatatcca tcgcctctaa aactggtcgc gccatctaat tttcctccta
    32821 aataaattcc ttactcaatg cctaaggtaa tactaccttt ttattattcg tcgtgactcc
    32881 ttctaagcct ttatctgtaa aatttattcc tccagttttt attcttcttt atccgctaca
    32941 aattcaatga tttcaacgct gacaatataa tcactcaaat atgaccaagt atcttcaaca
    33001 gataattcgt ctccattatc atccgtaagc gtcattttaa accattcatt accttcatca
    33061 tcttcaggta gcaagccgaa tccaattgga ttatcagtca atttttgatc gtataaatca
    33121 tcctcgtcta atgctaaaac atctaatcct ttacaatttc cgcctcttct aaacgtcaca
    33181 aatcctttgt aatcccaacg ttggaaagaa accttaatcg tatgtgttcc ccatcggcta
    33241 ttcggatcat aatttttcat ttgttgtaac ctcctcataa tcagttagta ctataaaggt
    33301 atctttgttt ttatccaaaa aatctaataa atcttttgta gaagatatct cttcatcaac
    33361 ttcaccataa taaatacctt cgataattgt tgctatatct gaaaaactaa tttttactgg
    33421 acatttatcg tctaccgcat tcaataccga caaaattgct tctttttctg caatctctcg
    33481 tttgaatttc tttggagcta atcgcatgac actcaagtca ttttcaagct tttcaatcca
    33541 tgcagtttta ctcattttcg caaatttttg aatcattcct ctaccacctt tgctatctac
    33601 taaattagtt ttacttcata acgaatattt ttatcgtttt tcgttcactc aaaaatatat
    33661 aaaataaatc gactcgttta tggcgtccga aacgagaaac cctagtagaa aacggtaaat
    33721 gctcgattga tccaaagatc aacgtcttcg caagtatcta catgtactgc agcacacgac
    33781 acaatttctt gtgcgtcctc cccattacga gaagacctac tccttattcc atgagtttgc
    33841 gtcaaagcct agatccctta gtagctgctc atgattaatc taggcgctat tctaacttgt
    33901 ctaacttcta aattccctct tgtttactct gtgcatatta tttacacaaa ttcatttcac
    33961 cactatccaa acaagggcat ttactcgatt gaccaatttt ttacgttacc aatcgtaaaa
    34021 acctaccccg tcaccccccg aaaggaattg ttgatagatc aagtctactc cttatttttc
    34081 tagtgattct aagttccttt agaagtctcg acaatatttt caagcaagcg aaaaacgata
    34141 aaatacaagt tatgagaatg atcaattcag ccgatcaaac gccccatccc ttttgcggta
    34201 caacgcaaga ctccattttt tcttcgacca atcagtacac cgttttggtc ataaacatgt
    34261 ttgattgttg gatatagctc ttttaagggg ataggatctt ttaaaattat ttgcttttgt
    34321 ttcccaatgt aataaatcat ttcctctgag actttgtctt tctggattat atatttctta
    34381 cactgcacaa cggttttact ttggccagtt gatagtttct tcgtgatttt ctctgtagcg
    34441 gtacaccgtt taccgtagtg acaaatatct ttgagactca aaattcctgt atatgacata
    34501 aaataaacca cccaatttct ttaaatattt ttaacaagtt tttcttgtta tttttcaaaa
    34561 atagagaatt gattttcgtt catgacaaac aaaaaagagg gaacgcatcc aaacatgcgt
    34621 tccctctaat ttcagcgttc gtcatacggc aaagagccac acggacattt ttataacatt
    34681 gtgcttaggc atagattcat cgtaatatca cgtattttta accttaacac agccaaaaaa
    34741 agtggtatca cttttgaata tctgttatac taaatccatt gcgggtaata ccgtaacact
    34801 gaaaggttaa cgcattaagg gccaaatctt aatgccgtgt atgaacgctg acgaaggtta
    34861 ccagccttcc gagttcatac cgcctttctt ttttttgtat tttcaattgt ccaaatttat
    34921 catccaaaag tgcagaacga aaatcaattc atcacaagca tagcagacaa ttaaatttat
    34981 acaaaaaatg taagcgaata atattcgtat tgcctgaaaa gaagtaatat caagattttt
    35041 agcttgttta aataatgaaa ttatctctct tcgcgtatta ttataactca aaaaaaagga
    35101 gtatgcaaac atacgttccc ttttctatat ttgaaattaa ttatttcttt taacacttat
    35161 gacgtaaaag gctacgtttg tgatttttat cataatattt t
//
LOCUS       DAFEHS010000021        34448 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFEHS010000021
VERSION     DAFEHS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.021"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..705)
                     /locus_tag="LP327_001114"
     CDS             complement(<1..705)
                     /locus_tag="LP327_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL1725245.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
     gene            1393..1551
                     /locus_tag="LP327_001115"
     CDS             1393..1551
                     /locus_tag="LP327_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1725246.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            1623..2843
                     /locus_tag="LP327_001116"
     CDS             1623..2843
                     /locus_tag="LP327_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL1725247.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKTVFEAFQKGLKN"
     gene            complement(2936..3328)
                     /gene="rpsI"
                     /locus_tag="LP327_001117"
     CDS             complement(2936..3328)
                     /gene="rpsI"
                     /locus_tag="LP327_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL1725248.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3344..3787)
                     /gene="rplM"
                     /locus_tag="LP327_001118"
     CDS             complement(3344..3787)
                     /gene="rplM"
                     /locus_tag="LP327_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBL1725249.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4306..4773)
                     /locus_tag="LP327_001119"
     CDS             complement(4306..4773)
                     /locus_tag="LP327_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL1725250.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4893..6431)
                     /locus_tag="LP327_001120"
     CDS             complement(4893..6431)
                     /locus_tag="LP327_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBL1725251.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6511..7197
                     /locus_tag="LP327_001121"
     CDS             6511..7197
                     /locus_tag="LP327_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBL1725252.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7398..7925)
                     /gene="msrA"
                     /locus_tag="LP327_001122"
     CDS             complement(7398..7925)
                     /gene="msrA"
                     /locus_tag="LP327_001122"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL1725253.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(8009..8686)
                     /gene="pgmB"
                     /locus_tag="LP327_001123"
     CDS             complement(8009..8686)
                     /gene="pgmB"
                     /locus_tag="LP327_001123"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL1725254.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8683..9813)
                     /locus_tag="LP327_001124"
     CDS             complement(8683..9813)
                     /locus_tag="LP327_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL1725255.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9813..11996)
                     /locus_tag="LP327_001125"
     CDS             complement(9813..11996)
                     /locus_tag="LP327_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL1725256.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11993..13018)
                     /locus_tag="LP327_001126"
     CDS             complement(11993..13018)
                     /locus_tag="LP327_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL1725257.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(13063..13890)
                     /locus_tag="LP327_001127"
     CDS             complement(13063..13890)
                     /locus_tag="LP327_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL1725258.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13887..14954)
                     /locus_tag="LP327_001128"
     CDS             complement(13887..14954)
                     /locus_tag="LP327_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBL1725259.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14980..15816)
                     /locus_tag="LP327_001129"
     CDS             complement(14980..15816)
                     /locus_tag="LP327_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL1725260.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15813..16682)
                     /locus_tag="LP327_001130"
     CDS             complement(15813..16682)
                     /locus_tag="LP327_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1725261.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16752..18035)
                     /locus_tag="LP327_001131"
     CDS             complement(16752..18035)
                     /locus_tag="LP327_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL1725262.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(18049..19734)
                     /locus_tag="LP327_001132"
     CDS             complement(18049..19734)
                     /locus_tag="LP327_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBL1725263.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            20009..21034
                     /locus_tag="LP327_001133"
     CDS             20009..21034
                     /locus_tag="LP327_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1725264.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21629..25303)
                     /gene="rpoC"
                     /locus_tag="LP327_001134"
     CDS             complement(21629..25303)
                     /gene="rpoC"
                     /locus_tag="LP327_001134"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBL1725265.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(25361..28978)
                     /gene="rpoB"
                     /locus_tag="LP327_001135"
     CDS             complement(25361..28978)
                     /gene="rpoB"
                     /locus_tag="LP327_001135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL1725266.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29706..30674
                     /locus_tag="LP327_001136"
     CDS             29706..30674
                     /locus_tag="LP327_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL1725267.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30692..31597
                     /locus_tag="LP327_001137"
     CDS             30692..31597
                     /locus_tag="LP327_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725268.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31594..32400
                     /locus_tag="LP327_001138"
     CDS             31594..32400
                     /locus_tag="LP327_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1725269.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32536..33525
                     /locus_tag="LP327_001139"
     CDS             32536..33525
                     /locus_tag="LP327_001139"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBL1725270.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33842..34366)
                     /locus_tag="LP327_001140"
     CDS             complement(33842..34366)
                     /locus_tag="LP327_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBL1725271.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
BASE COUNT         9818 a         7325 c         6376 g        10929 t
ORIGIN      
        1 aataaatata gtatctccat aggctttttt taaattctct tcattaaata cagattctac
       61 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
      121 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca attcttttaa
      181 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
      241 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
      301 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
      361 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
      421 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
      481 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
      541 tccctgatga gggataatat ttaacattgc tttaagcaat gtagattttc cagcaccatt
      601 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
      661 taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta
      721 aatgataatg attatcacga tcatatggta tacaaaaatt atattttagt caaccctaaa
      781 ttttataatt tagatttttt tctgttgctg aagattagaa tgacaaaact atttgaaact
      841 acgcgtatag taagtagctt tcaaaatata taaaaaagga gcgttctcaa atgagaacat
      901 gctcctaaaa ctactttatt ggcgtatgga aaaattatac ttaaagtgga tagcgggttg
      961 aataagttga taagcatcaa aacatttata aaaattattc aagactatgt ctgttccata
     1021 tacaaatatg aaaatcagta aactccattt aacttttttc gtagtgtgtc tttttataca
     1081 tgtttatcgt tattaatagt gaataggaaa aaacctattt ttgatcattg acaattaaat
     1141 acaatttttg aataacgatc cctgctattc gagctagaaa ctcaagtacg ccatgacctg
     1201 attagcgaca aatacttaga gatttctttt tgttctggga aaacaaaatg ccatgacaat
     1261 agcagcaaat aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct
     1321 atgtgcttac ccaaagcaat tcaaaaattc taatcttttt taggagatga ttttataaaa
     1381 ttgagagtct acttgaataa aaaacaaacc tgtgagtttc taggaattga taataatact
     1441 ttagataagt ggattcgtat atataatttt ccagtaatca gaattgacgg tatagttcgc
     1501 tttgacatag aggatatcaa agattttatg aatacattta aaacaatttg aaaatttctt
     1561 ctcgccgcat atactaagaa tagcgaaata tactgcctta ctttttaagg gagtgaggaa
     1621 ttttgcctac aaaactttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat
     1681 tcagagaaat cactggaatt acatcagaaa agtatcaaaa agtatttcca actaggcaat
     1741 tggctattaa agccgaaaat gcaatgaaaa ggaaaattga aacagtttta agagaggaga
     1801 atgcaaattc actagaatta aaaggaaaaa taaaatttaa ggaattctat gaatcaaaat
     1861 ggcttcctag atatgaacta ggtcagacta ctagatcaaa aagagctcca agttacgtaa
     1921 caattagtaa tactaaagat atattccgat tgcacatcct tccaatgttt ggtgagtatg
     1981 caatgaacta tctaaattca aatactgaga taatatcaga tgaattaaca aaaaaatcga
     2041 aagaatacgc aaatataaaa attatcaaag gatatgtccg ttctatgttt gatattgcag
     2101 aaatactaaa ttatattgaa tttaaccgaa ctaccaaaat tattcaatgt atcactgcac
     2161 ctaaaaaaat tgctttagaa gaaagaagga tacgggaagg taatcaagca ttatcgtcag
     2221 aagagcttat tgcttggata gaagcagtaa acactgactt gaataatcac ttgcttactc
     2281 ttcatgacta tactcttttc atgcttaccc tatatcttgg tgatagaaaa agtgagacgt
     2341 atgctttgca atggaaatat atcgactttg agaaacaaac ggttcgcctt aaacatgctt
     2401 tggataaata tcaaaaaaag aaatttacaa aaggtcgaaa ggataccgta attcaagtac
     2461 ccgaaattgt aatggcatta ctaagcaaat ggaaatcagt gcaagctgag caattgttga
     2521 gattaaaaat taatcaaaca ccagatcagt atctctttac ctatactaaa ccatccggag
     2581 aagtaaattg ccctgttcac gctgattacc taaactatcg aatcaattca attaaaagac
     2641 gacatcctga attggctcat ttatctccac ataagcttcg tcatacctat gcaactatag
     2701 ctagacaagg cggagcaaat atgaatcaaa tatctaatgc tttaacacac tcagatattt
     2761 ctactacaaa gatatacgta aacacaccag atgtagttga taagactgtt ttcgaagcat
     2821 ttcaaaaagg attaaagaac taggatgaat gtgttctcga atgtgttctt aaactaaaaa
     2881 aagacacttt ccaaataatt gaaaagtgtc ttgaaacatt gatatacaaa gaatattaac
     2941 gttttgaaaa ctgtgaagct ttacgagctt ttttaagacc tggtttttta cgttcgacca
     3001 tacgtgcgtc acgagtaagt aagccagcac gtttaagagc taaacggaag tcaggatcaa
     3061 cttctagcaa tgcacgagcg ataccatgac ggatagctcc tgattgtcca gcgtatcctc
     3121 cgccgtttac gttaacaaat acgtcatatg cgccttttgt ttcagtaaca gcgaaaggtt
     3181 gattgattac ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct tttttattta
     3241 cagtaatttt gccagttcct ggtactagac gtacgcgagc aactgcgttt ttacgacggc
     3301 ctgtgccgat atattgaact tgtgccaatg aaattccctc ctattagatt aagtttgtga
     3361 tgtctaaaac ttctggttgt tgtgctgcat gtggatgttc tgctccaccg tatacgttca
     3421 attttgtaaa ttgtttgcgg cctaaagtgt ttttaggaag catacctttt acagaagttt
     3481 cgatcaaacg acgagaattt ttagcacgca attcgccagc agtgattgat ttcaatcctc
     3541 ctgggtagtt gctgtgacgg taataaattt tgtcagtcgc ttttttacct gttaatttca
     3601 ctttgtctgc attgatgacg atgacgaaat cgccagtatc caaatgaggt gtgaaagttg
     3661 gtttattttt accacgtagt acagatgcaa caactgttga aagacgtccc aatggaacat
     3721 cagttgcgtc tactacatac catttacgtt ctacttcgcc tggtttggcc atatatgttg
     3781 tacgcacgtt attttcctcc aatttaagtt ctgatgtttg acatacactt cgagtttccg
     3841 gggctctttg tggggcaaac aataccattt aacatattac ccgctaatct agtcaatgtc
     3901 aacgtttttc tgcttgattg aaaaatttat atgaaacttt acttcttttc tttcttttat
     3961 ataaaagggt cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca aaagtcttgt
     4021 cacaacccct tattttgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
     4081 atatccataa aacataagaa gctgtttttt aaaattgttt cttgtgactc ggagctgaac
     4141 ggcaggctta caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg tattagctca
     4201 aaaaacggca aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa
     4261 cgctgatttc acaacctctt tttcggtcaa gttcaacaac agtttttacg cgtcgatctc
     4321 tggcgctttg cccaaatgag cttggatcat ccagatacgt ttttctgtgg ctgttttgaa
     4381 ttcgataaag atatcttgtg ttggatcgtc gccttcttct ccagaaactt cgattccttt
     4441 ttggtaaaga tctgctagat aacgatagcc tgcaacaagt gtttccaaac gttctggaat
     4501 agtacgatcc cagcggccaa tttcatcagg gatttttgtg ttatctgcaa attcacgcaa
     4561 tgtagaataa ggtgaaccgt ctaatgtgat caagcgttcg gatacttcat ctaactgagc
     4621 gttcaaatca tccatgaact catccatcat tgggtgcaat gtaaggaaac ctggtcctct
     4681 catgtaccaa tgtgtttggt gaacaacaac agacatttgg cttagatctg caactaattg
     4741 atttaatact tctttggttt gttggaattt catctttctt gcctcctaac attttcttac
     4801 acttaaaata atagtagact ttcaatgaaa agtacaacaa aatcatttac ctgagaacga
     4861 ttttcagttc gcttttttat ccgtaaagcc aatcattttt cagaatcagc taattcttct
     4921 cgcagttgag caaattgctg ataagtctgt gctaaatgct tttgtgtcga aacaaataat
     4981 ggatagattt tctgatattt tttatgatca gtttcttgta ctccaacttg ctctttcaat
     5041 tctatcaatt cctgtgcttc actgatactt tggatctttc cgatgcttct ccagcctaaa
     5101 atcacagctc ctaatgcgga tgcttctgtg acagtaggga aaacaagcgg gtaacccagt
     5161 acatcagcca tcatctgacg aaaaatcgga gattgagcaa acccgcctgt agcacggatc
     5221 tctgtgagct gattttcctg gcaaactcct tttaaaatcc gataaaggtt gagacagatc
     5281 ccttctagaa tcgccttcat catttctttt tctgtatgtt gccgctgtaa accgataaaa
     5341 ctgccaaacg catctgcgtc ccaaagcggt gctcgctcac caagtaaata gggatgaaaa
     5401 atcagaccat tggcacccgc tggcacaaaa gcgatagccg aaagcagtgc atcatatgga
     5461 ttttctcctt tttctttagc agattgaacg acttcctgta aaaatgtatg ccctgcccaa
     5521 tcaaagatgc cagcaccatt gctagtcgct cctcccacta cccaatgttt ctgatccaga
     5581 acataacaaa acgtctccat ttctggatgg aactgaggct gagtagtaat aaaacgaagt
     5641 gctccgctcg ttcctacggt caaagcggcg atcgtatcac caaaagcacc taaccctaga
     5701 ttagaaagcg gcccgtctgc ccctcccaaa acaaatgata tatcatttgg caaacctagt
     5761 tctgctgctg tttttgatgg tagacctgtt acttgatagg tggaaggaaa gacttgcggc
     5821 aattgctcta ttcgaatgtt caagtagtca agcacttctt ctgcccattg gaattcatgg
     5881 atatcaaaat atccagttcc tgaagctgtt ccgtagtcgc tgatcagttc gccaaacaat
     5941 ctataaaaga tatagtcttt gataccgaca aaataagcag tttttttgaa taattcaggt
     6001 tggctgtttt gcagccactt gattttgttc aatggtgtca ttggatgaat cggcgtccca
     6061 gtctgccgat aaaattcttt gccaagcacg gtatatttca gtttttctgc ttcgtccgct
     6121 gctcggttat ctgcccaagt gatcattcga gataacggtg ttttctcttc atccaacaat
     6181 atcaggccat gcatcgcaga ggagaaggag acaaattgaa tgattgtgtc tgaaggcaac
     6241 tgtttaatta cttcttttat aacggtgcag acaccttgaa agatcaattc ctgatcttgt
     6301 tctgccatcc cttgtgtttc ttggatcaaa gggtagcgaa aataagcgga tgctgcttct
     6361 tgggcttcat ctgtgaaagc aactgctttg gtttgtgtgg ttccgatatc taatccgatt
     6421 gaaacgttca ttttttcacc tcaaatttta tgtatgtaca aaaatttagt ttaagcttgc
     6481 gtatcttggt tttgaaaagg gggagatggc gtgttacttt attttatcat tggttgctgt
     6541 atcggatcat ttttatgttt aaccgcccag cggattccgt taggtcactc tattatttat
     6601 cctagatctc attgtgtcaa atgctgtcgt tcgctttctt ggtatgaatt gattccgatt
     6661 ctctcgatca tcgtccaacg atttcgctgc cgctattgca ggtgtcggtt gcccttctac
     6721 tatttgttgg cagaagcctt atgtggtggt ctttttgcct ggttctttac tttttcttcc
     6781 gcaagaaatt tttcgacgtt catatggatg ctttcggctt tgctattttc gttgatggat
     6841 ttattttact tcatggtcca ttctcacacg ctattctgtt cttgggcaat tttatggatt
     6901 ttttggttgc aggccggcgt cttccaatgg caaagtgtgc tgttaacgtt tatcgtaggc
     6961 ggtgtgtgcc ttcgctatgg tcaatcctat ctcggagctg gcgatactct gttgatgctt
     7021 tcttggagtg gtggcttgtc tctagaagag ttgcttcaga tcttgtttct tgctagtctg
     7081 tttggaatat ccgttttttc tgtttacttt atctgtcata aaaaaaagtt ggaaaagctg
     7141 cctttcattc cttttttgag cagtgcgttg ctgattgtcc ttcatcttag gtattgaaaa
     7201 aaggttgtga caaacgactt gtcacaacct ctgatatata gtgttctctt accaaatcct
     7261 tgtgacttgg agctaggcga caggttcaca acctctccac taaatggtgt tcaatcagct
     7321 gaccctcagt attagctcaa aatgctggtt ttacaacctc tgatatacgg tgttccataa
     7381 gtagcttttt cggcttctta tttcttgacg ccccaatgtc gacgaaggaa acctgcacgt
     7441 ccagaacctt ctcgataaag attgtaatgg agcttgtttt tcttgtaata atcctgatgg
     7501 taatcttctg caggataaaa ttcgcttctt ggttcgattt ttgtcacgat tggcttatcg
     7561 aatcgcccgc tgtcttgcaa tgcttgtttt gaacgctctg caatatttcg ttcttcttca
     7621 tcattatagt agatcactgg acgataagaa tcaccgcgat cagcgaactg tcctaatgcg
     7681 tcagtcggat cggtttgctg ccaatagatt tccaccaact gatcataaga aataacttct
     7741 ggatcatatt cgatctctac tgcttctgta tgccctgttg tgcctgttgt tacttgttca
     7801 taagttggat ttggtacatg cccgccagta taaccggatg tcacagaaat gatccctggc
     7861 tgctcttcaa atggtttgac catacaccaa aagcatccgc ctgcgaaaac tgcttttttt
     7921 gtcatataaa atcccccttt tttctcatca taccttgatt tccaaaagga ggataacgtt
     7981 ttgcttgtga cagaaaaaac ggaaactatt atatacgtga tacggcttct gataaatgcc
     8041 aaaacgaacg tacttgatca aaaacggtta aatcaggaat cacatattca gcagaaggaa
     8101 gatttatgtt ttcacctatt cccaatacat gcatgttacc agccaaagct gcttgacaac
     8161 cagcttctga atcttcaatg accaagcatg catttggcgg tacattcaat tgctgtgctc
     8221 ctttgagaaa aacttctgga tcaggttttg cttttgatac ttgcgtgccg tcaataagga
     8281 catcaaaata acttgtcaaa ttcgtttttt ctaaaatgag gcgtgcattt ttgcttgctg
     8341 atcccaatcc gatcttgatg tttgtttttt tcaagtactc aagaacagct attgcaccag
     8401 gcaaaacgga tgtttcatcc attttttgaa tcgcttgaag atatagattg tttttttgct
     8461 cagcaaaggc tgatttctct ttttctgaga atttctgttc catttttcca aatgacagca
     8521 atcgctctag tgactcggtt ctggatattc ctttcagttg ctcgttttct ttctcagtga
     8581 atggaatgga cagctggtta gctaaaacca gccacgcttc gtaatgataa cgagctgtgt
     8641 ctacaagaac gccatctaaa tcgaatagtg ctgctttgat tttcatcgat tacctccttg
     8701 aaaatgaacg acttgaagta aagatgcgat gtttttagct gtgcgatgaa gattatcagt
     8761 ggcttgctgt aaagcttctt gtaaaggcac agctgcagga aggatcggaa aaacggccga
     8821 aatgccaaca tctgctgctt tttcgcttcc tttaccaaca cttccgcaaa tagctatcac
     8881 aggaacgccc tctggtactc ttctagcaat cccaactggc gcttttcctt ttagactttg
     8941 tccatctaga cgtccttcgc ctacgattac gaggtctgct tgctgtaccc gtttatcgaa
     9001 ctccagtaaa tccaatacgt agtcgatgcc agagactatt ttccctgaag caaatgctaa
     9061 cagtcctgca cccattccac caccagcact actgcccgat gcctcgatta cttgaggggc
     9121 aaatctcata taaaaatcat gcatggcttg gtcagccact gctaattcat tttctttcaa
     9181 gcctttttgt ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa gattagtaac
     9241 atcagtaatg acagtgattt tggctttaat cagctcaata ggcacgttcg ttttatcaac
     9301 tgtagcaatt ttacctagat tttctcccag tgcttctact ttttgattat cttgatcgta
     9361 aaaagcataa cctagacctt ctgccatgcc gatgccgcca tcgttggtac tacttccgcc
     9421 aacgccgatg atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca gttctcctac
     9481 tcctcgcgtg gataacctaa gtggctgttg ttggtttagt gggacatctt tcaatccgac
     9541 gatttctgcc atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga tttctttgtt
     9601 tttgccaaat gcattagtaa cgactgctgt ttgccgcttc aaccttaaac tgtctgctaa
     9661 tgatgaaacc gttccttctc ctccatcgcc tataggtagc agatcatatt cagcctgaac
     9721 aaaaacttct tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg tcagactttc
     9781 tttgaaagaa tctggcgcaa tcagaatacg cattattttc ccctccgttc caagatcagt
     9841 tctttttcca gctggtattg gtctccattg acaaaaatag gtaatgtttc tttcgtctct
     9901 ttttttatta ctatttgttt ttttgtcaaa acaaatagca acctgtgatt tttccaaata
     9961 aaaggaaaag aaattttttc ccatgctgaa ggaagtcttg gagaaataga aagagttcca
    10021 ttcgttactg taatacctga aaaaccaaat ataactgcta gccatagtgc tcctaatgaa
    10081 gcagcatgaa ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat caggcacgct
    10141 tcttggaata aatgatacgc ccattccatt tcttggatac ggcaagcctc tatggcgtga
    10201 atcgctttac tcaacgatga gtcgtggatt gttttgtttt catagtaatg caaattctta
    10261 gaaacgattg gttgagaaaa atactcaggt aataagtata acagcatgac aagatctgct
    10321 tgcttgctga cttggagttc atttacttcg ctacgggcat aatcaagtaa aatagactgg
    10381 ctgccaggac gttctttgta agtggtcaaa tcaataactg gcttactgag atacgtgtca
    10441 tcttgcggaa tcagctgttc tttgttaggc actggcaaat ataacttatc taaaaaattt
    10501 tgacattctt tcaaaaaaag cggatcgcca gcttgatatt taacagctaa ggccacatta
    10561 taatgcgcca gatagttcgt atatgcatta ttgtcgatat attctgtgta ttcatctgga
    10621 ccaatgactc tgtgtatctc taaatgatca tcgactttta cagctctgct cagccaaaaa
    10681 gctgctgttt catgaatgag cgtttttccc tttgtttcta aaaacgattg atcctctgtt
    10741 gcttggacat actcttgaac ggcataagca aggtctgcac tgatatgatg ttctgcggca
    10801 gctgaagcaa cttcttgtct tgttccggtt ttgatattca atgcagcaaa tttgggtgtc
    10861 tcttcctgac cagtgtgtgc gctttcccat gggaaaagtg caccttgata gtgataatct
    10921 cttgcttttt gccgtgcgcc ttctaatcga tcataacggt acagcagcaa attctttgct
    10981 acttcaggaa actgatataa aaagaaaggc aggataaaaa tttccgtatc ccagaaaaca
    11041 tgccctttat aaccttcgcc agttagtccc ttcgccccta cactaacact tggatcattg
    11101 cggggagtca taatctgtaa atggtaagca gcgaagtcca tcgctaactg atcgaaagaa
    11161 gaatcgcctt ggatcgttat ccgatgctga tgccaaaaat ctgtccaata agacatcgac
    11221 tgctctaaaa tttctgtata actaacgctt ttctgggctt gggcaatact tttcttaaac
    11281 gggtctgttt gctgaagaga ggtagagagc acgatgatct tctccagcgt aaaggacgta
    11341 ttttttctga tcttttctga aaaggaagcg gtgatccggc gattttttgc ttggaataca
    11401 cctgctttat tgaatttagc tgcaatccca atatcaattt tactttctgt tgtctggtag
    11461 gatgcactga tcaaatcgtc accaaatact cgcagttctt tttcaaccaa ctgctgtgta
    11521 ccaaaattgg tttgctgtcc atcgataccc gttttcacat ctacttggca gtcttcggtc
    11581 aacggcttga tgaccacttt cagggctgcc tgatggagat tattttgatc agcaacacga
    11641 caagtaatga cttccacttt ttttccttga gcagtttcaa tgagcgcata gcgtttaagc
    11701 tctcccattt ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt ctcaagagaa
    11761 aaatgttcac cgtcgatttt tatttcccat ctgatgacat ctgggagatt cactaactcg
    11821 gctgattctg ctcctaaagg tcggttatat acaccagctg caaacattcc ccttacttga
    11881 ttccgatagt cttcctcctg gcttcctctg ataccaaaaa atccatttcc aatggtcatc
    11941 tgactcgcat acttattgat atactctaaa tcaaactgtt tttcttctac tttcataagg
    12001 taataacttc tcctgtttct gccgaacgat ataaattttc caccagattc tggatgacat
    12061 atccttgcca gccatctgca atcatctgag tctggtcacc gagcgcccga ttcacaaaat
    12121 gagccatact tagttcatgt gggtcattaa ctggcgtggt cttttgtaag gaaaccagct
    12181 ttccatctac atcttcgaat agttcgcctg gatacagatt agcacctgcc tgatcgccac
    12241 aaaaagccac attcagtttg tcttctctga tattcaaagc aaacgaagta ttcagttgga
    12301 tgatcttgcc gttttctaat tccagtgtgc caaaaattgc atcttctact gtgaatttgc
    12361 ttggatccca tttcccgaat ttccctgaat tttttttcgt accaattttt tggaagctgt
    12421 acgcccgaac gctttttact ttaggaaatc cagtaatata catcgctgta tcaagcatgt
    12481 gaatgccata atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata aacgttcccc
    12541 agcctggaac accagaccga cgaagcgcat tcgcttctac gtaataaata tccccgagaa
    12601 catcaagatg attcttgatc attgttgctt cgtcagaaaa acgatgctgg aatccataag
    12661 ccaaaatctg cccgttttta tcggctgtct ctgccatctt tcttgcttga tcagctgtca
    12721 tagctggtgg tttctcgcac atcactgaag cattagcttc gagggcagcc aatacatgtt
    12781 cgaagtgaaa gcgattgggt gtacagacac tcacgatatc gggagaaatc tcattcagca
    12841 tttgtgtaac tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag tcttctgctc
    12901 gctggctgac tggatcaacc acagcggcca attctaagac atctgatctt ttttgataat
    12961 aagcagcatg tactttttct gctacttgtc cagcgcctat gattgctact ttcttcattt
    13021 atttctctct cctttgctta tttacttatt gttattcttt ctctattctt tctctaaatc
    13081 atttaaatga cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt cctgataaat
    13141 tttcacttca ttttctcctc gcaaacgaca ttcatatacc atataacctt catactcatt
    13201 ttgaagtagc gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg gttggtagcg
    13261 atgattatct gctaaatgga tatgacccac atactctttt tgcgtagtca atgcttttga
    13321 aatatcatct tcttcaatat tcatatgata aaaatcagcg atgatctgca catgtttcaa
    13381 ttgattagag tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat ggtcttggta
    13441 tcggttcaaa ggctccaaat aaataaccgt acctgtttct tcggctactt tatctaaata
    13501 acgaagtgat tcgctgactg ctttatagtc tccttctttg cttcggggtg aaaccatcgg
    13561 cggtaatcga taagtgaaca ttccccaagc tgctgggata acgattcctt tgcctccaac
    13621 ctcgcttagt gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac gccgttcttc
    13681 gataaagtca ccgatccacc catcgtagcc gttacaagct gtcactactg ggatccctgt
    13741 ttctttgatt gcctgtttga ctgtgtcgat attttctatt aacaacttac cgtcgatttc
    13801 gtattcttca aatccacagt ctttgataaa tctaaatttt tctaaaatgt ctcttgggaa
    13861 aaatgcttga tcttgcgtac cgaatttcat tgttctcctc ctcggacttg aacgcccatt
    13921 ttgatacttt cttctgggtg ctggtctaca taacgcatgt aagcttctgc gctttctgca
    13981 aatggcacaa ctggatcaat gatttcttca caatccaagt aaccgttcat caataattcc
    14041 caacatgtgt cctcgattcg tttacggttc catcttgggt aatctgggtt tggttcacta
    14101 gatgctcgag caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc aagattaaac
    14161 ccagctggaa aaggtttagc aaaggcaaca taagaaatga tcccgccata gccgatccct
    14221 attaaagaag cttgtaatgc ggcagcactg ccactcgttt caataactga atctactcca
    14281 cgtttatctg tcaattcctt tagacgaaaa ccgacatctg ctttcatagg atctagcgtg
    14341 taatcggcac cgtgtttttc tgctaattct ctacgctgcg ctatgggatc gatcccaacg
    14401 atggtagtag cacctgcttt tttagctaat tgaatcgcaa tttgcccgat agcgcctaaa
    14461 ccgaagattg ctacagcatc acctgctcga acatgtgctt cccggatacc acctaaagcg
    14521 aattgagcag gatcgtaaca tactgcattt ttccattgat ccaaccgatc aagtttacgt
    14581 agtttataat tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc ataagtgcag
    14641 actaactctc ctttttgata ctctgtcacc aaactgcccg tttcttcgat cgtaccgaca
    14701 atcatatttc ctaattgaaa ttcgccaaat accacaccgc gttggcttcc ttcttctctt
    14761 ggcaagaata ggcgccattc ttctgaaaat tcttcatcga taaatggact taatcctcta
    14821 aagtcgacaa tttctgttcc tggttttgga gtagcgaaaa ttactcggat acgtacttcg
    14881 ttcttttcga tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg ttctaatgca
    14941 actaattttt tcatcttttc tttcctcttt cttttctttt tatcctttta cgccgccttc
    15001 gactaaatca cctttaataa atcgttcaga taacgcatac ataatgatga ctggtaaagc
    15061 ggtcaccaat gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac tgaataatgt
    15121 gttcaaaccg attggtaaag taaatttttc ggaggtcgac aggaaaatcg aagcaaataa
    15181 ataatcattc cacgaaacca taaagcaata aacgaagaca gaaatgattc cagaaatagc
    15241 caatggtata atgatccgaa taatgatctg gatccggcta agaccatcga tcattgctgc
    15301 ttcttcaata tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata tcgcagtagg
    15361 aagtgtttga acgaccatcg tgataatcag tgctgtattg gtatcataca aacctagacc
    15421 ggaaatgatt ttaaataatg gaacaacgat caaaataccg gaaaacatat aaacggtgta
    15481 aaagctagca ttgattttcg agcgagcttt gaagcgtagt tttgacaaag cgtatgcccc
    15541 gctgatccca atcactactg cagttacgga ggctatcaag gagatcacta aactattttt
    15601 gaaatacatg atgaatggaa aaatctcagc attgaaaata tcctgataat gttcaaaagt
    15661 ccattctttc ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat ccttgaatga
    15721 tgtcatcagc atgatcaaaa atggaaacaa attgacagct aatagcaaca aaatcgtcgc
    15781 gtaaaataag attttcctgc tctttttatt tttcatttca agtccacctt ctttcgtgag
    15841 agcaagacga ccgctgcaat aatgatgaac aatagaatcg atatagctgc tgcttttcca
    15901 agatcattga acgcaaaggc tgtctgatac aaataaacgc ctaagatatt gactttattc
    15961 gtcagtagat agacatctgt aaacatatag aacatccaga tagatcgtaa aacaatcaca
    16021 gttgctagta ctggtttgat tgccggcagt gtcacgatct tgaacttctg ccaagcattt
    16081 gcaccgtcca tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa aatagccaaa
    16141 aatgaaataa acgcatacgg atagtatctc cagatagcaa acaaaacgac cataaagaag
    16201 ctagcaactg gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa cctatctact
    16261 aacagatcat tgacgattcc ataactatta ttgaacatat atttccaagc aaaaatcaaa
    16321 gaaatagatg gcgtaacata acttaataaa atcagtgaac gcaccgtttt cctaaatctg
    16381 aacttgcggt taagcaaaat agctacggct aatcctaaag ctgtactgcc tgctacaact
    16441 aagaatgtgt aagcaactgt taaacctaaa gaacgataaa attcactatc acttaaaatt
    16501 tcaagataat tgcttaatcc gataaaaacg gcgtctgagt taggattcag cggcatatcg
    16561 aaaaaactga tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc taataaaagc
    16621 atgcttggtg ctaaaagtcc catggctaat tttaagtcag aatgtttcct tggacgattc
    16681 attgaaacct ccccctgata atctgccaat agctatctac ttttttcgaa caccattggc
    16741 agaacttctt gttatttcgt atttttttgt gcgtcagcta aagcttgatc gacatcttcg
    16801 tttccgacag ttacttggtt cactgctttt ccgatcacac ctgagcttgt gatatctccc
    16861 attttcgtga aattcttttc cccgactagt ccaaagactt gtacttcatc aaaagcacta
    16921 gcaatctcag atggcagctc gccaaaagac ttgatgactt cattttcttt gtaaccatcc
    16981 agttcgacta cttgtttatt cactggttga gcaccaccag gagacatcaa gacccacttt
    17041 tccatgtttt tcggttgaga taagaaagct gtgaatgctt cagcagcctc tttttgtttc
    17101 tcgtctagac cagaagaaat cgtcaatcca gatactgtgc catagacagc ttgatctttt
    17161 tgtgttggga tagcaaaacc gatattttct gagttccccg cttcataaac agaaggcaaa
    17221 atgtaagttg aatagattgc cattggtgct gttccattca tgaaagcatc tttgatttct
    17281 gtcacatcat tagagcctgg catcgtgtat tgggataagt tttgataaaa cgacaatgct
    17341 tctctcatct tttccgtatc gatcgttacc tcgccttcat catccaaaac atttgctcca
    17401 tttgacaaag caaattgaga gaacgcttgc tcactcattg ttccttcagc agtaggcata
    17461 gcaattccat attgtttatt ctctttatct gtgaaatgtt gagcaatttt taaaatatca
    17521 tcccaatttt ctggttctga aaaaccagct tctgataatt tttctttgtc ataccaaata
    17581 ccttgaaccc agccgctaat tggtgcagca atatagcttt ttccatcttc tgaacgaaca
    17641 aggtttttgg ctccgtcata ataattatct tctccagctt cctcgataac gttttggact
    17701 gccgtctgat caatcagttc atctttatcc atcactttag caaaatcctg actgacttcc
    17761 attacttctg gtaatttacc ggaacgagct aacgtaacga tttttgtatt gaacgctgat
    17821 tcttctaccg gcacttgctt gattttgatg tctggatttt ctttttcaaa atcagcaacc
    17881 agttcattga tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa ttgaatcgtt
    17941 gtctgttgtg attcgttaga gctttgactt tcaccacaag cagtaagaag aaatccagta
    18001 gctagcagac aggtagtaac aataattttt ttcatttttt tccacctctt attttattat
    18061 tttcagccac atatattcat acggctggaa tatcaacttt cgttcttgac tgcaatggat
    18121 aatttgtcca gaaaacaggt ctaggtaatc tccttttggt aaaagataac ctgctgattg
    18181 attggaaaaa ttatggataa ctaagacttt ctcttccttc gcttcgcgaa taaaactgat
    18241 aaaagaatct gctgagtcca gtaagtaaat caaactattc ggatggaagg ctttttcttt
    18301 ttttcgaata gatagcatct gggtcaattc accatatact gctgttctta atggatctgt
    18361 ctgcaactct ttctcaatat ctgctaacaa aaatttttgc cgattgattt ttcgattctc
    18421 tcctgttttt tctacgcctt tgtaatcatt tcgacttccc agcacacttt gaatataaat
    18481 tgccggcacg cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc gtttgcttct
    18541 ccattccgtc gtatctgttg gagaacttaa cgcatctaga tacgtagcgt ttatctcata
    18601 tggactggtc gttccatctg ggtttttctt gtagtttact cgggcaccct ctttcttcag
    18661 ctgttccact aattcaagga tcgttttttc cggcagaata cctcgtaccg gattcaatcc
    18721 gataccgtca tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg cgaaatcgat
    18781 ggtttttaac cattctttca acttacttgt attacccgaa cgaatcgctt gaagggtcaa
    18841 aggtggtagc gaaaactgat agaccatctg tgcttcattc tcgccattcc caaaataact
    18901 gatattatct ttgtgtggca catttgtctc agtgatcaat accgttcctg gagctgcttc
    18961 atcagcgatt gcacggaata attggatgat tttatgcgtt tctggcaggt gtatacaaga
    19021 agttcctagt tctttccata aaaatcccac agcgtccaag cgaataaatg aagcgccttt
    19081 ttctaagtaa aacaaaagaa tatcgatcat tttcaataaa acttctggat ttttaaaatt
    19141 caagtctact tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc cgtttttgtc
    19201 aatgaacgtt gtaagtaacg ggctggtcct tggtctagtc acgctgcttg tatctgtatc
    19261 cagtggcaaa tcaataaaaa aatctttgta cgtatcattc aagtttaaat attcctgaaa
    19321 ccaagtactt tcggcagaca tatgattgca gacaaagtcg aacattaacc gtgtttcgct
    19381 tctcatttct tctatatctt cccatgtgcc caaacggtcg ttgattgcct gataatttac
    19441 aacggagaaa ccatcatcag atgtatatgg gaaaaatggc aagaaatgta caactctaaa
    19501 tgtatctgcc aaatactttt gataaaattt cttaaatgta cgcaaagttg gttgctgctc
    19561 ttcaaaaaat tgatccccat aagtaatcaa gaaaatatct tcttcgtccc aatgcagttt
    19621 gcctgcctgg cctttcttct gctgataaac actgactttt tgttcaatca tagaggcggt
    19681 tttctcagac aaatctgagt cgcgataaat cgcttgtaaa agttttgtat acatcggatt
    19741 cccctttctt ttttgggagc gttcccaacg atttgatcat attatggaaa acgcttactg
    19801 tcaatagcat ttttttaaat aattttgata tacacaaaaa tacaaataag aaagcttatg
    19861 tatcaataat taatagcttc ttaaaaaaat atggtctctc atgatcatgc aagctattcc
    19921 taaattttca aaatcataga caaaaaagaa tgtctattgt actatggaat tattctaaaa
    19981 cgctcccaaa aaggaggtgc atataaaatt gaaacctact atttatgaca ttgcgaaaaa
    20041 agctaatgtc tcaaaatcaa ctgtctccag agtattaaat aaccagccaa atatctctga
    20101 taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg aattatgaac cgagcaaaat
    20161 tgcaagagga ctgtcaggaa atggttttga cgcgattcta gcattatctc atcgtacttc
    20221 tcatacgacc tatggcaatc cttttttttc agatgttatt caatccattt ctgccgtttc
    20281 tgaacaaaat aacttcgact tgattctaca aacggcaaaa acaccagaag aagagctaga
    20341 aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga attattattt taagctctac
    20401 gatggatgaa acacttcttt ttgaattgga tcaatacaat attcctattg tggtaatcgg
    20461 aaaagtcgga aacgactaca cgaacatcta ctcagtggac acggataatt tccaggatag
    20521 ttacgatttg gtggaaacac tgatcaagaa tggacaccgt catatcggct gtctccatgc
    20581 tccgacagaa gtttatgtat ccaatgatcg tctcaatggt taccgtcaat gcttatttga
    20641 tcaccaccta gatatccgta atgaatggat tatagacggc ggctttcgct tagaagatag
    20701 catggcagca atgaatgatt taatgaagct acgagaaaga ccaactgcga ttttttctac
    20761 cgatgcatta aaatcattga gtatttacaa agtggcagaa caatctggaa agcagatacc
    20821 tgatgatctt tcgatcgcag gattcagtga tcccttattg tcctccctat tttcgccaca
    20881 gttgactcgg atagacattc cgacaaatca gttaggaatc aaagccacag aacttttatt
    20941 ccaactaatc caccaccaat accctgaaga aaaaagaatc attctttcaa ccgagattac
    21001 tctcacagat tcgatccaac aacaagaaat gtagaacaga attataaaag aacagaaaca
    21061 ctgaggataa gtcagttact ctgttctttt ttcaatcact catccctcgt taccccctcc
    21121 cggtatttta gcgaattttt cttattttcc atctgtttta gatagctgtg gataactata
    21181 atgtttgtgt taaactattt tttccacagg gaagaaaaaa ctatttttaa ccacgttgtg
    21241 tataagtttt tatagttcgt ttttttaaac acatttaccc ttatttcaat ccatgttatt
    21301 tggggatact tatctctaaa acataaatag aataacagac atgtctactg gttttgtttt
    21361 attcgaggaa ttattcaata atagctagca aaatattaaa aataaccgct ctatcttccc
    21421 atcaatttat tttgcattca aaaaatgatc tatctttact tttgttgatt gacttctctt
    21481 ggaagatgat cgttccgata ccttaattgg atacgaaccg atgttgttaa gatcctttcc
    21541 cacttttctt ctagttagac aaattatttt agacaaacca aaaaagaggt tgtgacaata
    21601 atttgtcaca acctcttttt taaaatatct attttgtttg attgttcatt gcgtcttctg
    21661 cagccatttg cgcttcaata tctgaaatac tgtagacatt ttcgcttgct acaccgactt
    21721 cttttggttc catgtcacga taacgttgca taccagtacc agctgggatg atcttaccta
    21781 taataacatt ttctttcaaa ccaagaagag gatctttctt accgcggatc gcagcatccg
    21841 tcaagacacg agttgtttct tggaatgatg cagcagataa gaaactattt gtttctaatg
    21901 atgcttttgt gatacctagt aagacaggtc gagaagttgc aggaacgcct cctgagacta
    21961 gcgtattgta gttttgatct ttaaagtctg cgatgtctaa taatgtacct ggtaagatat
    22021 ctgtatcacc tggatccatt acacgaactt tacgcagcat ttgacgaacc attacttcga
    22081 tatgcttgtc gccgatttct accccttgca tacggtaaac acgttgtact tcacgaagca
    22141 agtagttttc aacagataat acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc
    22201 cttctgtcaa tggtgcacca cggtggatca tatcgccttc tgctactttc atacgtgctg
    22261 tataaggaac agtatacgta cgtgtatctg tttttccttt gattgttact tctttggtac
    22321 gtgttgcagg atcttctgag atatcgatga catcccctgt tacttctgtg atcactgctt
    22381 gccctttcgg attacgtgct tcaaagattt cttggacacg aggaagacct tgagtgatat
    22441 cgtctccggc aaccccacct gtatggaatg tacgcatcgt caactgagta ccaggttcac
    22501 cgattgattg agcggcgatc gtaccaactg cttctccgac ttcaacgtca gagccagttg
    22561 caaggttgcg tccgtaacaa tgtttgcaga caccatgttt tgtattacat gtgaagacag
    22621 aacggattgt cactgtttcg acacctgcat caacgatttg ttttgcgatg tcttcggaaa
    22681 tcaattggtt tcctgcaatg atcaattcat ttgtttcagg atgacgtact tctttacggg
    22741 tgtaacgtcc cactaaacga tcttctaatg attcgatgat ttcatttccc tcgcggatgg
    22801 cttggatttc aagacctcgg tcagtaccgc aatcttcttc acggatgatt acatcttggg
    22861 ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg
    22921 tcatcccttt acgagccccg tgagttgaga taaacatttc caagacggat agtccttcac
    22981 ggaagttcga aatgattggc aattccatga ttcgaccatt aggagcggcc atcaaaccac
    23041 gcattccggc aagctgtgta aagttggaaa tgttaccacg agctccagaa tcagacatca
    23101 tgaagattgg atttttcgct tccaaacctt ccatcagttt ttgttggatg ctgtcttttg
    23161 cagcattcca aacagcaatg acacgttcat aacgttcgtc atctgtgatc agaccacgac
    23221 ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt
    23281 cattcagtac actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt
    23341 accctaagtc tttcatacgg tcaagcatct tagaagtttc tgttactttg aatcgtttga
    23401 agacttctgc gatgatgttt cctaagtttt tcttcttgaa cggtaatact aattcttgct
    23461 ctttgatatg tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga
    23521 cggtcaagtt gaagtctgtt ggttcattca gataagggaa ttcaggaggc atgatctcgt
    23581 tgaagatgat cttaccaact gttgtgatca tcgtacgttc tttttgccat tcagtaaatg
    23641 gtttttcacc taatgagtta gggttcacac cgatacgtga atgtaaatga acatatccgt
    23701 tgcgccatgc gataacggct tcgttttgat ctctgaagac cattccttcg ccttcgcgtc
    23761 cttcttcttc cattgtcaag tagtagttac ctagaaccat atcttgagaa ggagtaacaa
    23821 ctggtttacc atctttaggg ttcaagatgt tttgagcagc taacatcaac atacgtgctt
    23881 ctgcttgtgc ttcttcattc aatggaacgt ggacagccat ttggtctcca tcgaaatcgg
    23941 cattataagc ttcacataca agtggatgca aacggattgc gcgtccttgt actaaaactg
    24001 gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt
    24061 gttccttgat tacgtcttct aaaacatccc atacttcgtc ttcttgacgt tcgattttac
    24121 gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac aagctcacgc atcacgaatg
    24181 gtttgaataa ttcaatggcc atttcttttg gaagtccaca ttggtacatc ttcaagaaag
    24241 gtccaacaac gataacggaa cgacctgagt agtcgacacg tttacctagc aagttttgac
    24301 ggaaacgacc ttgtttccct ttcaacatat gagaaagaga tttcaatggg cggttacctg
    24361 gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa tgcatctact gcttcttgca
    24421 gcatacgttt ttcattttga acgatgattc caggtgcatt caagtctaac aaacgtttca
    24481 aacggttatt acggttgatt acacgacggt aaagatcatt caagtcactt gttgcaaaac
    24541 gtccaccttc taattgaacc attgggcgta aatctggtgg aataacaggg ataacgtcca
    24601 taaccatcca gcttggttga tttcctgaag cgcggaatgc ttctaaaata tccaaacggc
    24661 ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc aattcggctg
    24721 cttctgcatc tagatcaaca ttatcaagta attgtttaat cgcttctgca cccatagcag
    24781 cttggaattc ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt
    24841 gttttttctc taaattcgta tttcctggtt cgatgactac atatgaagca aaataaatga
    24901 tttcttccaa agcgcgtggg ctcatgtcta atacaagacc catacgtgaa ggaatccctt
    24961 taaagtacca gatatgtgaa acaggagctg ctaattcaat atgtcccatg cgttcacggc
    25021 gaactttcga acgagtaact tccactccgc atcgatcaca aacgattcct ttataacgga
    25081 tacgtttgta ttttccgcag gcacattccc agtcttttgt aggaccaaaa atccgctcac
    25141 agaacaaccc ttcgcgttca ggttttaagg tacgatagtt aatcgtctca ggttttttta
    25201 cttcaccgta agaccagctt ctgatttttt cgggagaagc caaacctatt tgcatacttt
    25261 cgaatttatt tacatcgatc aaaaggggtt ccctccattt catttaatgg caaaccgcaa
    25321 gacggctgat cttcgaccgc cttgcagctc atagaccgga ttagtctaaa ttatcttctg
    25381 cggtttcaaa gttttggatg acatcgtctt tttcatcttc cacttgttca gcagctttct
    25441 tttctaattc tttggcagtt tgctgttcgg caaattttgt caaagcatca acagtgatca
    25501 aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga
    25561 cacgcatgtc cagacctagt gattgcaatt ctttcaccaa tacgcggaat gattccggta
    25621 cacctggttt tggaattggt tcacccttga cgattgcttc gtatgttttc acacgtccga
    25681 ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt ataagccgca ccgtaagctt
    25741 ccagtgccca tacttccatt tcaccaaaac gttgtccacc aaattgagct ttacctccca
    25801 atggttgttg tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga
    25861 ccatgtgagc aagtttgatc atgtacatca caccgacaga cacacgtcca tcaaatggtt
    25921 cacctgtacg tccgtcgtac agaacagttt tcgcatcgct ggccataccg gcttcacgaa
    25981 ctgtttccca tacgtcttca tcacttgcac catcaaatac tggcgttgct acgtggatac
    26041 ctaactgacg tgcagccata cccaagtgta attccaatac ttgcccgatg ttcatacgtg
    26101 aaggcacccc taaaggattc agcatgatat cgattggtgt tccatcaggt aggaatggca
    26161 tatcttcttc cggcataata cgggatacaa cccctttatt tccgtgacga ccggccattt
    26221 tatccccttc atggatttta cgtttttgaa cgatataaac acgaactaac atgtttacgc
    26281 ctggtgacaa ttcatctcca gcttcacgag taaagatttt cacatcgtga acgataccgc
    26341 cgccgccatg tggtacacgt aatgatgtat cgcggacttc acgagctttt tcgccaaaga
    26401 tcgcatgcaa tagacgttct tctgcagata attctgttac cccttttggc gtgactttcc
    26461 ctacaagcaa atcgccatct tggacttctg caccaatacg gatgataccc atttcgtcta
    26521 aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct tcaggtccta
    26581 attttgtatc gcgggcttct gattcatatt cttcaatatg gatagaagtg tatacgtcat
    26641 cttttaccaa acgacggctc atgatgatcg catcttcata gttgtaccct tcccaagtca
    26701 tgaaaccaac taaaacgttt tggcctagag ccatttcgcc ttgttccata gatggtccgt
    26761 ctgccagagt atcccctttt tcgactttct cacccaaatg aacgattgga cgttggttgt
    26821 agcttgttcc ggagtttgaa cgacggaatt ttgttacatc gtatttatct aatgcaccgt
    26881 tgtcgcggcg tacgcggatt tcagaagcat caacgtattc aacgacaccg tcatgtttac
    26941 ataacaaagc agctcctgag tcatgcgctg atttgtattc cataccagta ccaacccatg
    27001 gtgcttgagg attgatcaac ggcactgctt gacgctgcat gttggcaccc atcaaggcac
    27061 ggttggagtc atcgttttcc aagaaaggaa tacatgctgt cgcaacggca actacttgtt
    27121 taggtgatac gtccatgtag tctactttat caatagatac ttctaagttt tcactttgag
    27181 cacgagccat aacaacgtct tgagcaaatg ttccatcttc attcaatggg ctgtttgctt
    27241 gggctacgat gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac
    27301 gacctgtttc acggtctaca cgacgataag gtgtttcgat aaatccgaat ttatttactt
    27361 tcgcataact ggacaaacta ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg
    27421 gacacatacg gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac
    27481 gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt gtgtgtcaat tcgccaagag
    27541 ggtttgtttg atccatgaac tgtgacaact gagaagaacc aaagaattct ttgatacttg
    27601 ccacaactgg acggatattg atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg
    27661 acattctttc acgaaccaca cgttccatac gtgctagccc gatacggaat tggttttgta
    27721 aaagttcacc gactgaacgg atacgacgat ttcctaagtg gtcgatatca tcgacattgc
    27781 cgataccttc catcaagttc aagaaatagc tcattgaagc aatgatgtcg gctggacgaa
    27841 ccgtttttac agctgcttct ggatagccgt ttccgatcac gtttacttca cgttcagggt
    27901 ctcttggtga aaatactttg atgacttgga cagtcattgg atctgtgacc acaccgtctt
    27961 ctgatggata gtaagtcact gaatttaggc cattatcaat gaaaggagct agtgtttcca
    28021 tgacttggtg agtcagaact gttccttttt caacgatgat ctcgcctgtt tctggatcaa
    28081 ctaatgtttc agctaatgtc agattcaata gacgtgtttt caaatctaat tttttgttta
    28141 ctttgtaacg accaacgttt gctaagtcgt aacgttttgg atcgaagaaa cgagcattca
    28201 gcaagttacg tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt
    28261 ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg
    28321 tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc
    28381 ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt acggtcgata cgtacataag
    28441 agatgtcttt tgcatcagtt tccatttcta gccaagctcc acggttaggg ataacagtag
    28501 aaccgaatcc ttcttttccg tttttgtcga cttttccatg gaaatagaca cctggtgaac
    28561 ggactaactg agaaacgatt actcgctctg ccccgttgat gatgaatgtt ccttgttctg
    28621 tcattaatgg gaaatcaccg aagaatactt cttgtgcttt gatttcgcca gtctcgcggt
    28681 ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt
    28741 ctgctactgt atactttggt tctttcaatt cataatctac aaattccaaa gatagattgc
    28801 cattgaagtc atcgattggc aaaatgtctt caaacatttc tctaagtccc tcatctaaga
    28861 accattggta agagtctgtt tgaatttcga tcaaatttgg taattccaac acttcactga
    28921 ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc
    28981 tcttcacccc tctaaaatgt cattcaaaaa gactacccga tcaattgtta caataatatt
    29041 tcaaatatta tttttaggta acaacagaca ttttttgcgg cttttaggca aaaaaaaacc
    29101 aaaaatagaa cattctcttg aaaccttact tcgtgagctt ctacttttgt ttccttatat
    29161 taaagccgaa acggacaata tttcgtttct gccttttctt catcagatag tttttgcaac
    29221 ttcatatact acacttattt tctataaaag tcaacacttc ctggcgtttc ttctgctttt
    29281 tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa ttatctgaca attcttaatt
    29341 gacagacctt tttatctcgg ttatacttta gaaaattcat tttgtggagg aacttctatg
    29401 cgaaccaaca tcaaccaaat cgcaccaaca caactgaata acttttactt tagctacttt
    29461 agataggttt gtattcatca tgatggatac aaacgttaaa cgacagtttg tatctatgat
    29521 ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc
    29581 agtgaaaata caaagtaaaa gtatcaagca ttctactttt tcaatttatt ttccgacttt
    29641 tcaatccatt tggacatttt gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa
    29701 aagaaatgaa aaaaaatata atcatcggat taggcgctgc cctgctacta gctttaggag
    29761 gttgcgcaca aaaagacaat tccgcagagg catcagatgg acaaaaaaca attggcgtat
    29821 tgcaacttgt acaacataac tcgttagatg ctgcctataa aggattcaaa gaaggattag
    29881 cagaagaagg gtataaagaa ggcgataatc taacaatcga ttatcaaaat gcccaaaaca
    29941 atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa agaagatcct gatttgctac
    30001 ttggaatcgc cactccagca gcacaatctt tattaaatga aacaaccact atcccgatga
    30061 ctgtcacagc ggtcacagac ttggaacaag cccatttagt cacatccaat gaaaaacctg
    30121 gcggcaatgt gactggcacg acagatatgg tgccaatcga aaaacaaatc ggcttactgt
    30181 taaatatcgt gccagacgcc aaaacaatcg gcatcatgta taacaacggt gaagcaaact
    30241 ctaagatcca agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag
    30301 tactgacagc aaattccaca aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg
    30361 tagatggaat ctatattcct actgataaca cgttcgcaag tgcggcaagt gttgtcggtg
    30421 aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg
    30481 gcggtctagc aacagtagga atcgactacg aaagcttggg caaacaaaca gggatcatgg
    30541 cagcaaaaat cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt
    30601 tggaactgta tgtcaataaa gacatggcca aagcattagg catcaatcca gacagcatca
    30661 aagaagctga ataaaacaca ggaggattct catgacaaat ttgctctctc ttttcttatc
    30721 atctgcatcg caaggggtct tatgggccct acttgcgatt ggtgtatacg tcactttccg
    30781 tatcttggac attgccgact tgactgctga aggaagcttt cctttaggtg cagggatcgc
    30841 ggcagtaagt attacaaatg gctatcatcc attgacagct tgtcttctag gctttttagg
    30901 gggtgcggca gcagggcttg tttccggact gcttcacaca aaattgaaaa ttcccgccct
    30961 tcttgcaggg atcatcacca tgactggttt atattctgtc acatcccgtg tgatgggtgc
    31021 gccgaacatc tctttattgg gtcagccaac gatctttcat tgggtcgagt cattcggcat
    31081 aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt
    31141 acttgttttg ttcttacgta cagaaacagg acttgctatt cgtgcaactg gcgataatct
    31201 ggcaatgagc gaagcaaacg ggatcaacac agataatatg aagatcatcg gttatatgat
    31261 ttctaatgga tgcattgctc tttctggctc cttacttgcg caaaacaacg gctttgctga
    31321 tttaaattct ggtattggaa cgatcgtaat tggacttgct tctattatca tcgcagaagt
    31381 tttgttacgt aaccagccac tatttctccg tctcttgact gtcgttttag gtgcggtcat
    31441 ttatcgtttt attttagcgt tagtcttcga actgaatgtt gaaccaagtg attcgaaact
    31501 tgcttctgct ttcgtgctag tgatctgtct ttctttcccg cagatccagc aaaaattcca
    31561 tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac attggaaaat
    31621 ctccatcata cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa aggcattaat
    31681 ctttccttag atcccggcga cttcgtgacg atcatcggtg gaaatggggc tggtaaatct
    31741 actttgctga acagcatcgc cggtacattt tctgtcgatc agggtcaaat tttattgaat
    31801 ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga aatccatcag tcgcgtattc
    31861 caagatccta aactagggac agccgttcgt ttaacggtag aagaaaatct tgctcttgct
    31921 atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga ccgaacattt
    31981 ttcaaagacc agctggttcg gctaaatttg gggttggaaa atcgtttatc ctcagaaatc
    32041 ggtttgctgt ctggaggaca acgtcaagct attacgttat tgatggccac attacagcgt
    32101 ccagaactga tcttattaga tgaacacact gcagcgcttg accctgctac ttccaaaacg
    32161 gtgatggaat tgacagaaaa gctgatcact gagcagaatc tcactgcttt tatggtcact
    32221 catgacatgg aagacgcgat ccgctatggg agtcgactga tcatgctgca tcaaggaagg
    32281 atcgtcgtag atatccagca agaggaaaaa cagcagctca cagtgcccca attattagcg
    32341 ttgttcaaac aaaacagcgg cacggcatta aaagacgatc aagtactttt atcttcataa
    32401 tcagaaagag gatgggacaa aaaatgaacg cccaccctct tcttttttct ctttccttct
    32461 ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa actattttgt tctctccttc
    32521 ttcctgatag aatggatggg aaaggagctt gatgccatga caacgaaaga caaggtactt
    32581 caattattga aaaagacacc agattttctt tccggagaaa agttggcaca gcaactggaa
    32641 gtttcccgga caagtatttg gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt
    32701 gagcacgggc ctacagggta tcgttacctg ccctctgacg tactagatgc aaaagaaatc
    32761 cagcaagagc tacggtcgtt tattcctgat ttgaaagtac aaacgatgtt gacttctgaa
    32821 tctacgatga aagatgccaa actggcagca gctcaaggag attctgtttc gaagttattt
    32881 gttgctgata cacaagaaca agctcgtgga cgttttggac ggcctttttt cgctgaacct
    32941 ggacgaggaa tctacatgag tctcttgctc catccagaca aacggtttga tgaattgcct
    33001 caatatacga tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt
    33061 tctgcttcga tcaaatgggt caatgatatc tacatggatg gaaaaaaagt ctgtgggatt
    33121 ttatctgaag cagtcagtga tatggaaagc ggcaggatca gtcatatcat cattgggatc
    33181 gggcttaatt tttctgttcc tcaaaatcat tttccagctg atctccaaga aaaagccatc
    33241 tctgtctttc ccgacggaca agctagtgtc acaaggaatc agctaatcag caaaatctgg
    33301 caagaattgt tcacttatct ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa
    33361 tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt
    33421 gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa caaatgaaca gaagatgagt
    33481 ttgtcttctg gcgaaatcag tctcacatct atccgtacgc aataattatc agatttcaca
    33541 aaaggacacc tgatctcata cagtgtcctt ttttctgaat tctcttcaaa agactctcct
    33601 tattctatca gctgacagtt tctctatcct ctagaaaaag gtattctaaa aatatctata
    33661 aagcatggta cactttcttt aaatgaatca atgcaaaaaa caagagagtc atcagtttac
    33721 acgatcaaaa gagatagcag gccgcttcct ttttcaatct tatagataga ccatgggtaa
    33781 cgataaaaac aaaaaagaac ttgcgactta ttttgccgta agttcttttt tagctagtgc
    33841 cttaatctct taatcccaac aaacgtctcg catagatcac ctgaccagag tgcatagcag
    33901 catcatcaat aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt
    33961 caattacttc atctagctcg gaaggttcca gccttcttaa gtaagaaata gcaaaatcag
    34021 cagctcctcg caagtatcct agcagatctt ctatttgatt agcttggatc tgctttgctt
    34081 gttccagact atgcttccaa tcttgttcgt tatctgctat cgtcatcgga aaccggcttt
    34141 cccagtcttg actatgccaa attggagaag ttcctgctaa atcagcaatc tggaaatcta
    34201 attctcgagc ggtatgccaa gccagccaag tcattgattt gatctgcggg gctctatctt
    34261 ccactggaaa acgattggcc tcctcaatag agacgccttc aaacaaacgt tcaaaccgat
    34321 cgtatgcccg atccacactc tcgcatagca tatctaaata atccatttat tttcctcctt
    34381 tttatttatc ctacttactt tttcctaatt ctaggatacc tacttatatg ttactacaca
    34441 cccttatt
//
LOCUS       DAFEHS010000022        33949 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFEHS010000022
VERSION     DAFEHS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.022"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="LP327_001141"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="LP327_001141"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBL1725272.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="LP327_001142"
     CDS             1013..2260
                     /locus_tag="LP327_001142"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBL1725273.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="LP327_001143"
     CDS             2336..2680
                     /locus_tag="LP327_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725274.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="LP327_001144"
     CDS             complement(2797..3123)
                     /locus_tag="LP327_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725275.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4697)
                     /locus_tag="LP327_001145"
                     /pseudo
     CDS             complement(3496..4697)
                     /locus_tag="LP327_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4814..5329
                     /locus_tag="LP327_001146"
     CDS             4814..5329
                     /locus_tag="LP327_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL1725276.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5528..5974)
                     /locus_tag="LP327_001147"
     CDS             complement(5528..5974)
                     /locus_tag="LP327_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL1725277.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6021..7499)
                     /gene="msr(C)"
                     /locus_tag="LP327_001148"
     CDS             complement(6021..7499)
                     /gene="msr(C)"
                     /locus_tag="LP327_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL1725278.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7853..8482)
                     /locus_tag="LP327_001149"
     CDS             complement(7853..8482)
                     /locus_tag="LP327_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL1725279.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8466..8930)
                     /locus_tag="LP327_001150"
     CDS             complement(8466..8930)
                     /locus_tag="LP327_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL1725280.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9417..10685)
                     /locus_tag="LP327_001151"
     CDS             complement(9417..10685)
                     /locus_tag="LP327_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL1725281.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10916..11653)
                     /locus_tag="LP327_001152"
     CDS             complement(10916..11653)
                     /locus_tag="LP327_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL1725282.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11720..12289)
                     /locus_tag="LP327_001153"
     CDS             complement(11720..12289)
                     /locus_tag="LP327_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL1725283.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12417..13289
                     /locus_tag="LP327_001154"
     CDS             12417..13289
                     /locus_tag="LP327_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL1725284.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13631..14503
                     /locus_tag="LP327_001155"
     CDS             13631..14503
                     /locus_tag="LP327_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL1725285.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14500..16458
                     /locus_tag="LP327_001156"
     CDS             14500..16458
                     /locus_tag="LP327_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL1725286.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16709..17560)
                     /locus_tag="LP327_001157"
     CDS             complement(16709..17560)
                     /locus_tag="LP327_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL1725287.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17683..18150
                     /locus_tag="LP327_001158"
     CDS             17683..18150
                     /locus_tag="LP327_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL1725288.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18413..18895)
                     /locus_tag="LP327_001159"
     CDS             complement(18413..18895)
                     /locus_tag="LP327_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL1725289.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18995..19858)
                     /locus_tag="LP327_001160"
     CDS             complement(18995..19858)
                     /locus_tag="LP327_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL1725290.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20456..21304
                     /locus_tag="LP327_001161"
     CDS             20456..21304
                     /locus_tag="LP327_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725291.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21475..21816)
                     /locus_tag="LP327_001162"
     CDS             complement(21475..21816)
                     /locus_tag="LP327_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBL1725292.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21902..22609
                     /locus_tag="LP327_001163"
     CDS             21902..22609
                     /locus_tag="LP327_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL1725293.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22596..22919
                     /locus_tag="LP327_001164"
     CDS             22596..22919
                     /locus_tag="LP327_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL1725294.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23008..24045)
                     /locus_tag="LP327_001165"
     CDS             complement(23008..24045)
                     /locus_tag="LP327_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL1725295.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24492..25475)
                     /locus_tag="LP327_001166"
     CDS             complement(24492..25475)
                     /locus_tag="LP327_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBL1725296.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25891..26661)
                     /locus_tag="LP327_001167"
     CDS             complement(25891..26661)
                     /locus_tag="LP327_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725297.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26654..27589)
                     /locus_tag="LP327_001168"
     CDS             complement(26654..27589)
                     /locus_tag="LP327_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725298.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28183..29832)
                     /locus_tag="LP327_001169"
     CDS             complement(28183..29832)
                     /locus_tag="LP327_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL1725299.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30027..30923)
                     /locus_tag="LP327_001170"
     CDS             complement(30027..30923)
                     /locus_tag="LP327_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL1725300.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30916..31596)
                     /locus_tag="LP327_001171"
     CDS             complement(30916..31596)
                     /locus_tag="LP327_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1725301.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31597..32379)
                     /locus_tag="LP327_001172"
     CDS             complement(31597..32379)
                     /locus_tag="LP327_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725302.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32372..33289)
                     /locus_tag="LP327_001173"
     CDS             complement(32372..33289)
                     /locus_tag="LP327_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1725303.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33292..33576)
                     /locus_tag="LP327_001174"
     CDS             complement(33292..33576)
                     /locus_tag="LP327_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725304.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT        10144 a         7118 c         6065 g        10622 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttc
     4441 aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg
     4501 taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt
     4561 tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca
     4621 actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga
     4681 ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt
     4741 aaatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag
     4801 aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc
     4861 acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca
     4921 gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga
     4981 cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga
     5041 atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta
     5101 tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca
     5161 gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa
     5221 acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact
     5281 ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag
     5341 aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt
     5401 ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc
     5461 tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag
     5521 tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga
     5581 tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag
     5641 agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca
     5701 ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat
     5761 ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa
     5821 tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt
     5881 gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg
     5941 taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca
     6001 cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct
     6061 tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac
     6121 aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca
     6181 ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca
     6241 atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc
     6301 tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt
     6361 ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat
     6421 acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct
     6481 aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa
     6541 aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt
     6601 cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt
     6661 ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc
     6721 gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc
     6781 ggtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt
     6841 ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct
     6901 cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata attccccgaa
     6961 aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa
     7021 tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt
     7081 cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca
     7141 taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc
     7201 tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt
     7261 gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc
     7321 ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca
     7381 ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt
     7441 tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata
     7501 tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa
     7561 atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact
     7621 aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata
     7681 ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa
     7741 agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta
     7801 ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga
     7861 acgtagtgat cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc
     7921 ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca
     7981 actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta
     8041 acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc
     8101 ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg
     8161 gcatcttccc tgattcgtaa gcttggttat agatttcttc gccatattcg taacattgca
     8221 tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga
     8281 tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt
     8341 gcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt
     8401 gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg
     8461 aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa
     8521 agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa
     8581 gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctc gattttcttc
     8641 tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc
     8701 gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc
     8761 tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg
     8821 atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac gattttttga
     8881 tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc
     8941 tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa
     9001 gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga
     9061 aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt
     9121 attgattctt atcaaactat agaagatagc tcttctgcag tagaaaattt tacaccgcca
     9181 aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat
     9241 gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg
     9301 gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg
     9361 tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa
     9421 taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc
     9481 ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa
     9541 attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt
     9601 aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat
     9661 aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag
     9721 cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta
     9781 tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc
     9841 agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa
     9901 cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg
     9961 gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc
    10021 aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca
    10081 ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca
    10141 aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg
    10201 gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt
    10261 gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca
    10321 tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc
    10381 ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct
    10441 gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa
    10501 attgtatttt ctgagacagt agctgactgc aaagtactct ctgtatcgtt ttcaattcct
    10561 gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact
    10621 gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa attctttgct
    10681 ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa
    10741 gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttgat aaatttgctt
    10801 tttctacata aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg
    10861 gtcgtttgaa taactattta acaagtaatt ctctttggat attatccgtt atggattata
    10921 cttcctgtgt cgtcggacgg ataatcatgt tactgacaga catccggtct ggcgtttcta
    10981 tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa tccccattct ttttgcgctt
    11041 catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag
    11101 ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt
    11161 ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa
    11221 catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg
    11281 gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc
    11341 attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca
    11401 atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa
    11461 aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg
    11521 cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata
    11581 gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg
    11641 ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc
    11701 tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga
    11761 ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa
    11821 tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat
    11881 ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat
    11941 ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc
    12001 cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct atactaatat
    12061 gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac
    12121 taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg
    12181 ctgctaaagg ctcttcgttt tcagcttgga tctgatggat cacttcaaag agcggatccg
    12241 ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc
    12301 cttttatcct tactttataa cggacggctt atcatgtcta atacttataa aacatactta
    12361 actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg
    12421 atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag
    12481 caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag
    12541 aacttggtgt gacacttttc actcggggct cccgagagat acagttgacg gattctggtc
    12601 gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt
    12661 taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa
    12721 tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc
    12781 atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt
    12841 ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc
    12901 aagatcgttg gggacttttg atacatcgaa atcatccttt agcaaagcaa accgtcatcc
    12961 atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc
    13021 aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact
    13081 tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg
    13141 ggatcatcaa tacttatggg acggaaaaaa tattcatccc actcgatccg ccattacaag
    13201 cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt
    13261 tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt
    13321 taacaacgaa taagataagc aaaagatcca acgattaaat aacataagtt tcttaattta
    13381 tttcttcctt ttatttctga taattccagt aaattactat aattataaaa aaagaattga
    13441 caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt catgtgacta
    13501 gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg tttttcgtgt
    13561 ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa
    13621 gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg tagatgacgg
    13681 tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa gaaatgattt
    13741 gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt
    13801 gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa cgatcattga
    13861 tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg ctttgaccga
    13921 ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca acaaactttt
    13981 aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg cagtagagta
    14041 tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata tcgcaatcca
    14101 tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg aagtgacgat
    14161 tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc attcagaacc
    14221 attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac aacgaaccca
    14281 tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac gaaaataccc
    14341 agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat accagctcgc
    14401 gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa gaagcgcaat
    14461 cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata tctacaacga
    14521 atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt actagtaggg
    14581 atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt acaagctggc
    14641 ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc actagggatg
    14701 tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga agttccagta
    14761 aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga atcagttgat
    14821 ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt gattgcggca
    14881 gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc
    14941 aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc agtgctactg
    15001 ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat tttgagttta
    15061 ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc tacaggattg
    15121 catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga tattggtaaa
    15181 tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg atttttccca
    15241 atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc acgtccagaa
    15301 aacaaagcaa aaacagcttc cttgatgcta gcagcagctt ttgcttcatt ctttactggt
    15361 gtgacagaac cacttgaatt ttcatttatg ttcgttgctt ggccattata tgtcttgcat
    15421 gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac agcaggattt
    15481 gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat tgcgaatcag
    15541 ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt tggctttaac
    15601 tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga tctttcagat
    15661 gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag caaaatctac
    15721 gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac acgccttcgt
    15781 ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac tggcgtacct
    15841 ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga agtacaattc
    15901 gttgcagatg agatggcaaa actacatggt ggtgctgcag cacgacctgc acaaacaaac
    15961 accgtggtta aaacagaaac atttgccggt gaaacgatcg aacaagatat ttacgctatc
    16021 gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg
    16081 atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc aatcgctggt
    16141 acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga tgctggtatc
    16201 gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc attcacttta
    16261 tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc
    16321 gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa tggcgacaaa
    16381 gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa aatcggctct
    16441 gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata aacaaacagg
    16501 agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc
    16561 aactccgcgg taataagtcc agctaaacga aacatatgaa gagctatttt ttatttgact
    16621 cttcttatta cttgatgcag aacagctttt tcacaaaatt tttcttcata ctcaaaacta
    16681 atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg
    16741 cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta
    16801 actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga
    16861 atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact
    16921 ctttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg
    16981 ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat
    17041 attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg aattgaccag
    17101 tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa
    17161 atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta
    17221 atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag
    17281 ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag
    17341 agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat
    17401 aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac
    17461 gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac
    17521 caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact ccctattctt
    17581 tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt
    17641 ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa
    17701 tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt
    17761 tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg
    17821 agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt
    17881 gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca
    17941 cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa
    18001 gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag
    18061 atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa
    18121 aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat
    18181 cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt
    18241 tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc
    18301 aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat
    18361 ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt
    18421 tattgactac cggtatccag atttctccca tatatttccc gttggtaact cccatttcga
    18481 ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa
    18541 aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgcttacaa
    18601 caagatacgt tccttttggg aatgggatga ttcgcgtagt gtttggaaca tcctgactcg
    18661 tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat
    18721 aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct
    18781 caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca
    18841 caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc
    18901 ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta
    18961 cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc
    19021 aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag
    19081 cttcgactaa cccataatct gaagaaggaa gccattctgc atagattttc ccccaagtat
    19141 cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta
    19201 tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt
    19261 gcatcaaact gcccttttct tccaagcggt ctccatcaaa tccataagaa acgttgatta
    19321 cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg
    19381 tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg
    19441 ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc
    19501 ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctactga
    19561 tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt
    19621 catatccata tctaaacgcc gtttctgtca ctcttgatcc atctagaaga tcgcgattag
    19681 ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa
    19741 agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa
    19801 gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat
    19861 ttatcttcct ccttgattgc ctttttatca tattatcaaa gaaagtcaat tacttgatga
    19921 atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa
    19981 gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa
    20041 ataaattttg gggaagacag attctaagta aacaaagcgg taaaaatatc ttttattccc
    20101 ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag
    20161 aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta
    20221 tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca
    20281 tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag aaaaggagaa
    20341 tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag ctactttttt
    20401 cttaaacgtt tctttatact aaatataaat aagtgtttca tgaaaggaga actagatgaa
    20461 taattggctt gagtattttc ctgaaaacgt tttggaacgc ggttacagct atcatctgca
    20521 cggttttgtc cgtcatttga actatacaag caagtacttg tccgcaacag tttctggcac
    20581 ggaagattat aaggtagtga tcacttggga tgaaaagacc aacatgactt gtgattgtct
    20641 atatgccata gaaggaaaaa aatgcaaaca catggctgct gtcttattcg cttacgaaga
    20701 acgtccaata aaaaaatcca actactcact ttccgaactc tcgtcccttg tttcctccgc
    20761 tagttcctct cttgtacggg agcttttaac agagattctt atagaacatc cgcactttat
    20821 cgaacgcttc aaagttaaga tgccatttca tgcaataaat tattcagata aattaacaac
    20881 tatcatccat aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa cagcgaagtt
    20941 catcatggag atgcgaaagt tcatccaaga agcagttgaa tcgcttattc agcaaaatgc
    21001 ctatcttccc gcattcgaat tgatcaatga agtgattgct actctcgaaa cattctattg
    21061 ggagcctgag gatgaacgaa cccttcttct gatagaggac tgttattatt tatggaaaga
    21121 attattagca gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt tcgtctgcca
    21181 agtcgatcat actgacgcct cttattcaaa acgctactct ataaaaatcc tgaaagaaga
    21241 ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaac taaagaaacg
    21301 gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg ataacaacca tcctaaatcc
    21361 acaagaaata cagcagacct tgctttaaaa acacgaatca tgcaaaggat gaagtaaaga
    21421 agacttaacg cgtacccaat attagtcatt tgactattag gtacgcgttt tgttttataa
    21481 gtcatgacaa atttccttcg ttgctggata gattttccgg cattgttgat agattttatg
    21541 atacttttct acattttgta agagaggttc cgctgcatct gtatattgga taaaattctg
    21601 acaacaactt tcaggtgtgt cgaaccaccc taaccctagt gcagcaatca ttgttgcgcc
    21661 taatgcaggt ccttcctcca tacttaaaca atagaccgtt gcatcgaaaa tatcagcttg
    21721 gatttgtagc catgcaagat ttttcgcgcc tcctcccacc gaaacaatac gtgtaaatct
    21781 gcgacccgct agttctttac gtttcaatcc caccattgac aaatgctcat taaaaattta
    21841 gaatattcac aagtttaacg acaattatga aaatttatgt cgtgctaaag gagttttgtt
    21901 attggatgaa aagttagata taaaaactgc gatcaaagac acgttaccta ccgttttcgg
    21961 ttatatcggt attggacttg catttggtat cgttgggaaa gctgccggat ttcatccact
    22021 agtcgtcacg ttgatgtccc tgctggtcta tgctggttct gcccaattta tcacagtcag
    22081 catgcttgct agtcacagcc cattgctttc catcgttttc tcgacctttc tagtcaattc
    22141 ccgaatgatt ctgatgagta tgacgattgc tccttatttc aagaaaaatc ggctgcttca
    22201 aaatctgctg attggcacat tgctgacaga tgaaagtttc gcactgggaa tgaataaatt
    22261 gaactatact ggacaaaagt taaactttcg ttggatgaat acagctaatt ggatttctta
    22321 tttgacgtgg gtgggttctt ctcttgttgg cgcactttta gggaatttta ttactgatcc
    22381 gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt atcgggttac tttatcttca
    22441 agtcatttct gatagaaaca cctctaaacg attacagttg atcctgattg gtctcacttt
    22501 gattctggta tatgtcggac tgatttttat cccaagcaac ttagtgattg tagttgtaac
    22561 actgattggg tgcggattgg gggtgtggat caaacatgcc ttcttttgag tttacattgc
    22621 tgactatttt aggttgtact ttagcaactt ggctttctag ggtcctacca tttgtcttac
    22681 tgaaaaaatt cgatttaccg caaccgttgc ttgagtattt aagttttgtc ccgatcgtca
    22741 ttatgtctgc tttatggttc agcagtctat ttacccaaaa tatcggacat ctgccgcaga
    22801 tcaatctcga aaatgctttg gcttcagttc ctacactttt agctgctatt ctttcaaaaa
    22861 gcttattggt gatcgtcttg gcaggaattc tttccctttc gttgattcgc ttattataaa
    22921 taaaaaaaca agagcttatg gcaaattact gtcatgcgct cttgttttta tcaaagtcaa
    22981 ataagaaaaa aatcggcgta ttcttgctta tccgttgaac ggaccactaa caacgacttt
    23041 tcctaacata tgtcctgttt ctacgtcttt ggttgcttgt ttcaagttct tcgcattcat
    23101 cccatctgaa tagaccttgc tcaatgtact gcggataatc ccttcatttg ctaaagcagc
    23161 aatgtgcgct aaagcttctc cttgcgtttc gatttcatag ttatagtctg ttttagcgaa
    23221 catatactcc cagtcgaagc tgatagattg gtttttccaa acacctatat ctaacggctc
    23281 tttgatccct acgatcgttc ccacatgtcc aaatggtcgg acaaggtgct tgatttgatc
    23341 aaagtagcgg gtaatatcga ataatacagc gacatactct acttgatcga ttcctaattc
    23401 acgaaaactt ttacttaagt cgtgatgata atcgattgga tagtctacac ctgttttttt
    23461 cagccattca aaattcttcg gacttgcagt cgcgtagact tctaggccag cccaatgcgc
    23521 tagttgattc aaaatcgaac cgacgccccc ggatccgttg atcactaaga tttttttccc
    23581 gcgattggca ttttcttctg gagttagacc aaatttttca aataacaatt cataagcggt
    23641 caatgaggtc aacggcaagg ctgcagcttc ttcatctgaa agattttttg gtgcaagagc
    23701 gacaatacgt tcgtcaacta gttggtattc ttgattgctt cctgctcttg ttgttgtacc
    23761 tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact tcgtcaccta cagcaacaac
    23821 tttcccgact ccatcaaaac ctaagatacg taaagcattt cttatcccgt tatcttggcg
    23881 aagtttcgta tcgacaggat taacagatac tgcgttgact tttaccagaa gatcccgtga
    23941 actgggtaca ggtatctgtt ctttttcatc aataaagctt tttggatcat cgatcggcaa
    24001 cccttcataa aaaccgacta ccttcattaa ttccttttct gacatacgaa ttcctcctta
    24061 gcttcatcaa gttggagcat ccatacagtg ttcatttcaa tatatatagt atgctatttg
    24121 ttccaccttt tttattcatc ataattattc tcttacttaa agtaaactat ttgcactttt
    24181 ttggttaaaa aagaggttgc gacaagactt ttgtcacaac ctcttataaa cggtgttctc
    24241 ctaccaactc ttcggtcaca agtcaaaata ttcatcaaac atgagaagct gttttttgaa
    24301 attgctcctt gtgactcaga gttaaacggc aggctcacaa cctctgatat aaacggtgtt
    24361 caatcaactg accctcggta ttagctcaaa aaacgacaaa atatgaagag ctatttttgt
    24421 ttggctgttc ttattacttg atacaggaca gctttttcac gacctctaat tcacggtgtt
    24481 caagcaagaa atcattgttc actgaaaagc gttggatgtt ctttttcaaa ggtctctaag
    24541 atctctcgta cttcatcagt agatttcgta ttcatcaact gaactcgcaa gtcatttgct
    24601 cctggaaatc ctttgatata gattttaaag aaacgatgta gtcctgtgat ggaacgcggg
    24661 atgatttctg aataatgatc ttgcagatct agttgcagac gaagtagtcc gatcagttct
    24721 ttttctgtat gttccctcgg gtctttttca aatgcataag gattcttgaa aatccctcgg
    24781 ccaatcatga tgccatcaac gccatatttc tcggccaatt cttctcctgt ctggcgatcg
    24841 gggatatctc cattgatcgt gattagtgtt tgtggagcga tttggtcacg taaagcaatg
    24901 attttaggaa tgacttccca atgtgcatct actttactca tctctttacg cgtacgcaaa
    24961 tggacagaaa gattggctat atcttgatga agtaggtgac tgatccattc ttccatctca
    25021 gacatttccg tatagccgat acgcgtcttg acgctgacag gcaaaccgcc agctttagct
    25081 gcttcaatca attgtgccgc tacttctgga cgaaggatca agccactgcc ttttccacgt
    25141 tctgctacat tagggactgg gcagcccatg ttgatatcga ttccttggaa gcccatttct
    25201 gccatagcaa tgctcatttc tctaaaaaat tctggcttgt ctccccaaat atgggcaacc
    25261 atcggctgtt catcttctgt aaatgttaat cttcctcgta cactgtcttt tccatctgga
    25321 tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga catccggtgc tgctgcatgc
    25381 tttacgacat ggcgaaatac aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa
    25441 ggctttggca gctctttcca aaaattagta gtcatttatt tgttactctc cattctatca
    25501 agcagtgaag tcgcaatctt tctcaaaaac attgtcatta tactaggaaa caaaaaaaga
    25561 agcaaagtga acagcttaaa agatgtttct gattgagcaa aaaaaagact gtgacaaaaa
    25621 tcttatcaca gtccattttc cggaataaat ggtgttcaaa agcttgcatt tgcggtaata
    25681 agtccaacca aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat
    25741 gcagaacagc tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta
    25801 ttcgctcaaa aaacaacaaa atatgaggaa ctattttccc gtttgtttct ctaatactca
    25861 ggtcaaaacg ctgatttcac aacctctttt ctaacttctc aatcctcttc ctttattgat
    25921 caaataccaa gtaacggcgt acaaaacaac gatgaataac acaagtacga tcatcgatgt
    25981 tgctactggc acatcgatcg ttccaaggaa tccgtaacgg aaaccggaga tcatataaac
    26041 gatcggattg acttttgaaa cagcttgcca aaagggcggt aacatagaga tggcatagaa
    26101 aacgccacct agataagtta acggctgcaa gacaaatgta ggcacgatcg atacatcatc
    26161 ataagactga gcaaaaatcc cgttgagcag tcctgctaat gaaaacaaga ttgccgtcat
    26221 caataatgta atgatgacca tgaaccatga atacacatga agtggtacaa agaacaagga
    26281 aatgatcgtt acgagagagc cgacgagaat actgcgaccc acaccaccaa tcacaaagcc
    26341 ccaaataatc acatgcgtag gaacaggtgc taccaagatt tcttcgatat ttttctgaaa
    26401 tttttgagag aagaaggacg aagaaacatt ggcatacgaa ctagtgatag cagacatcat
    26461 aatcaaacca ggaacgatga attccatata tgaaaaacca cccatgtcac cgatccgccc
    26521 accgatcatt tttccgaaaa taacaaaata caaggatgtc gtgatcactg gcggaaccag
    26581 tgtttggacc catatacgca aatagcggtt cgtttccttg atagctaaac tttttaatgc
    26641 agtaaaataa agattaaaca tttttctcct ccgtatgatt attttcttct gtgattttca
    26701 agaacagttc ttctaaacga ttcgacttat tcctcataga aagaactttg attccttgtc
    26761 ttgtaagctg atcgaagatt ccgttcaccc cttgatttcg ttcaacttcg acagcgattg
    26821 tctgctcatc ttcaaatgta caagcatatc cttcaatcac aggtttggta tcatatggcg
    26881 ctaaatcaaa gataaatgtt tcaaattgca gttttgacag aagctctttc atacttgtat
    26941 tctcgatcag ttcacccgat tggatgattc cgatgttgcg acaaagcatt tcagcttctt
    27001 ctagataatg cgtcgtcaaa atgatcgttg taccattttc gttcaattct cgtagaaact
    27061 cccacatctc gcgtctcaac tcgatgtcaa ctcctgctgt tggttcatcc aagatcagca
    27121 aacgtggttc atgcatcaat gccctagcaa tcatcagtct tcgcttcatt cccccggaaa
    27181 gcatacgtgc acggacattt ctcttttccc aaagattgga ctgcttcaaa tatttttcgc
    27241 tgcgtttcat cgcttctttg cgagagacac cgtagtagcc tgcttgattc acgacgattt
    27301 gctggaccgt ttcaaaaggg ttaaaattga actcttgtgg aaccaatcca atctgttgct
    27361 ttgctcgaac tagatccttg tccagatcat aaccgaagac attcactttt ccagatgttt
    27421 tattgactaa agaagtaata atcccgatcg ttgtagactt cccagctcca tttggcccta
    27481 atagcgcata gaaatctcct tctttcaccg aaagatcgat tccccgcaat gcttcgaccc
    27541 cacttccata gatctttttc aaattattta tttcaagcgc atttgccatt tatttcctat
    27601 ctccttatag acatgattct taattgtatc atggaataca gcagatgaaa aaacaacttg
    27661 gcttccacac aaaaacacac ctgctaacaa agaggagatg tgttttctag ttcatgatca
    27721 tcttcgatag aataactatc taaacaactc tggaactttt aaaatcataa aagtaccgac
    27781 agcaactact acgatgacat atagaagatc cttataactg aggttcttgt cttttttatt
    27841 catgttaagc tttcctccgt ccttttatcg aaagcttttt tcttttattt taaccgaaat
    27901 aaataaaatg gaaatagtta ttttcaattt ctgttttcgc tgtttgtttt acggagaact
    27961 ttgccatgga acgtgcgtct cgtcgctgat gctaagtttt ttcgataaaa gctgctagaa
    28021 ggaagacagc gtggtttatt ttgtatcgtc ttttgctaaa tcttccaaga tcatctggac
    28081 agcagactcg ttcccattga attctgccac ttttttggca tcaatgtact tcttttgata
    28141 atcgtctttt tggacccacg catcatgctt cgtttgataa agttaatcaa acggatacca
    28201 ctgacgctgt tcatcgaaca acaaactttc tttttgggcc tgttcaacta tggatttagg
    28261 ataattcaaa actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa
    28321 tttgaaatca aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc
    28381 acaataattc tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga
    28441 tggatactcc gcaagccacc gaacgactga agcggaagaa gcaatccgtt cgatcgtatt
    28501 tgttcctttt aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc
    28561 cagcaaacgt ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc
    28621 atcttcgatt gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat
    28681 cgctgtttgg atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga
    28741 ttttcctgat gcattggaac ccgtgactaa tgtattcttc tgccagttga ctggattaac
    28801 taccgcttct tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa
    28861 ctctggtcgg caagtgatcg gcatataggt gcgataattc aaaacggctg ctgcaacttc
    28921 caattctccc agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt
    28981 gagaacgaaa tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat
    29041 caattctgct tcactcccgc tcttcattcg aaatgaaaaa cctaatttag ggatgtctct
    29101 taatggttta gtttgctgtt tcagctcttt ttgaagtggt gtcgtcattt ttgctaactg
    29161 attggcggct gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt
    29221 ttgtttcttc actagataat agaccgtatt gaatactaag ccaccgattg ctagatagat
    29281 tcctagtact tgccctaaaa gaagcaaacc tacacccacc agcggaagca agccaagaaa
    29341 aataaataaa cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc
    29401 aaaattatga tctttttttc ctaaacgagc gaattgatat tggattcttt ctctcagttg
    29461 aggattttcc tgataaaaag caattagttt ttctaactga taatcttctc caaaatcaaa
    29521 gctacgtaaa cgctgataca acgcttctga acctacacta gaataggtac tgttgatccc
    29581 ttcaaatact tcgaacatat ccaagtcata ccaagtaaga tcgtcgattt cactatccca
    29641 tgaacggaat tttttctccg tgagccacgc atccttcaaa ctttcttctt tatcaaatct
    29701 cggttgataa ggaatttttc cccaattact acgaactgtt tcctttactt tcaaacgtga
    29761 aaaaatgtct aaaacaacta ataaaataac aactgcaata ataattaaaa cgataaattc
    29821 tctttggccc atatccctct ccgttccttg atttgttagt tttagtatag cactgttgcg
    29881 ctatttgaaa agggggtaca aaaataactg tcttcgttac ctaattcgta tcaatcacac
    29941 caaaataaaa acaccttcca aaaaagaaga tgtctttaaa acgagataca attttttgtc
    30001 attcacttca ggtaaattag agccaattaa accctccgca acttgacgga gatggagaag
    30061 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
    30121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
    30181 cgctggaaga ctttttcaac agaggtttgc gctcctggtt tgataggatt ggccactgtt
    30241 aatcgaatga atgaatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
    30301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
    30361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
    30421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
    30481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
    30541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
    30601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
    30661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
    30721 tcattttcat ccacccatgt attcagtgca tctactaggt ccattagatt cttttctcga
    30781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
    30841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
    30901 agaagcaaca gccacttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
    30961 agcatgtgcc tttgctagtt tccctcgaag attggaaata tgcatattca ctgttttctc
    31021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
    31081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
    31141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
    31201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
    31261 taacaacagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
    31321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
    31381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
    31441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
    31501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaagaatgaa cagcttcatc
    31561 gtcttcgata atcaaaatag tttccagcag gaacacctac ttaatgtcag atttttggaa
    31621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
    31681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
    31741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaacgaaggc
    31801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
    31861 tgtcaaagcc actggaacag ataatgtcgc taatatcagc agaagcaata caggaaatac
    31921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
    31981 tgtgtaagca atcatggcta gccccgtaat caaaaccacg ccaagtgttg tgatcaaaag
    32041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
    32101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
    32161 cagaagaatc catgcagcca ttttttccac cattaaaatc cctgttgcac cgttgatgac
    32221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
    32281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
    32341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
    32401 gtaagaaata atcttccagt ttttgcccaa ctaggtggat accatccaca ggaacttttg
    32461 cctctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
    32521 cacggctaga ttggacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
    32581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
    32641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc gtagtgagtg gcgatctgtg
    32701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
    32761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
    32821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
    32881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
    32941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
    33001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
    33061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
    33121 aacccataat agttctcata aaggtagact tacccgctcc attctcccca atcaaaccat
    33181 agatcattcc acgtttgatt tcgatcgata ctttatctaa tgctttggca gcaccgtatt
    33241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
    33301 tttcttattg ataagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
    33361 aagaaagtgt aaattttcca attgaatttc aagaaaaatc agactgtctc gcagacgtgg
    33421 ttgagttgtc tgccaatcaa agagcgcacc aaacagtgct aatccaagtg gactggccac
    33481 catggtaatc gttgttatcg ttgacatcag acgtccttgt atatgcgttg gaacttctcg
    33541 ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac ggacttatga
    33601 aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt tatgacaaaa
    33661 gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc ctcggtcaca
    33721 agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt atttcttgaa
    33781 gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga tgatacttta
    33841 tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc tacctattaa
    33901 catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgcca
//
LOCUS       DAFEHS010000023        32262 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFEHS010000023
VERSION     DAFEHS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.023"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(169..1188)
                     /locus_tag="LP327_001175"
     CDS             complement(169..1188)
                     /locus_tag="LP327_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1725305.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1210..2217)
                     /locus_tag="LP327_001176"
     CDS             complement(1210..2217)
                     /locus_tag="LP327_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL1725306.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2210..2875)
                     /gene="pgmB"
                     /locus_tag="LP327_001177"
     CDS             complement(2210..2875)
                     /gene="pgmB"
                     /locus_tag="LP327_001177"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL1725307.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2868..5162)
                     /locus_tag="LP327_001178"
     CDS             complement(2868..5162)
                     /locus_tag="LP327_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL1725308.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5422..7587
                     /locus_tag="LP327_001179"
     CDS             5422..7587
                     /locus_tag="LP327_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL1725309.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7891..8724
                     /locus_tag="LP327_001180"
     CDS             7891..8724
                     /locus_tag="LP327_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBL1725310.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8826..10526)
                     /locus_tag="LP327_001181"
     CDS             complement(8826..10526)
                     /locus_tag="LP327_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725311.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            11033..12193
                     /locus_tag="LP327_001182"
     CDS             11033..12193
                     /locus_tag="LP327_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL1725312.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12209..12652
                     /locus_tag="LP327_001183"
     CDS             12209..12652
                     /locus_tag="LP327_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1725313.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13135..13665)
                     /locus_tag="LP327_001184"
     CDS             complement(13135..13665)
                     /locus_tag="LP327_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBL1725314.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13770..14321
                     /locus_tag="LP327_001185"
     CDS             13770..14321
                     /locus_tag="LP327_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL1725315.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14474..14947)
                     /locus_tag="LP327_001186"
     CDS             complement(14474..14947)
                     /locus_tag="LP327_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL1725316.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15085..16557
                     /locus_tag="LP327_001187"
     CDS             15085..16557
                     /locus_tag="LP327_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL1725317.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16554..17264
                     /locus_tag="LP327_001188"
     CDS             16554..17264
                     /locus_tag="LP327_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBL1725318.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17616..17900
                     /locus_tag="LP327_001189"
     CDS             17616..17900
                     /locus_tag="LP327_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBL1725319.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18181..18600)
                     /locus_tag="LP327_001190"
     CDS             complement(18181..18600)
                     /locus_tag="LP327_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBL1725320.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18602..19186)
                     /locus_tag="LP327_001191"
     CDS             complement(18602..19186)
                     /locus_tag="LP327_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBL1725321.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19233..20708)
                     /locus_tag="LP327_001192"
     CDS             complement(19233..20708)
                     /locus_tag="LP327_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBL1725322.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20889..21200
                     /locus_tag="LP327_001193"
     CDS             20889..21200
                     /locus_tag="LP327_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725323.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21315..23651)
                     /locus_tag="LP327_001194"
     CDS             complement(21315..23651)
                     /locus_tag="LP327_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1725324.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23793..24461
                     /locus_tag="LP327_001195"
     CDS             23793..24461
                     /locus_tag="LP327_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1725325.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24464..25503
                     /locus_tag="LP327_001196"
                     /pseudo
     CDS             24464..25503
                     /locus_tag="LP327_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
     gene            complement(25740..26621)
                     /locus_tag="LP327_001197"
     CDS             complement(25740..26621)
                     /locus_tag="LP327_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL1725326.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26780..27508
                     /locus_tag="LP327_001198"
     CDS             26780..27508
                     /locus_tag="LP327_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBL1725327.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27664..27981
                     /locus_tag="LP327_001199"
     CDS             27664..27981
                     /locus_tag="LP327_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL1725328.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27974..28582
                     /locus_tag="LP327_001200"
     CDS             27974..28582
                     /locus_tag="LP327_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBL1725329.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28637..29428
                     /locus_tag="LP327_001201"
     CDS             28637..29428
                     /locus_tag="LP327_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBL1725330.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29566..30078
                     /locus_tag="LP327_001202"
     CDS             29566..30078
                     /locus_tag="LP327_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBL1725331.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            30104..30976
                     /locus_tag="LP327_001203"
     CDS             30104..30976
                     /locus_tag="LP327_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL1725332.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31152..31514
                     /locus_tag="LP327_001204"
     CDS             31152..31514
                     /locus_tag="LP327_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL1725333.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31523..>32262
                     /locus_tag="LP327_001205"
     CDS             31523..>32262
                     /locus_tag="LP327_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725334.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVKPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILG"
BASE COUNT        10052 a         5723 c         6633 g         9854 t
ORIGIN      
        1 gccagaatga ggttcattct gacccagatt tgattttgta caacccatca gtccttattg
       61 acaaagagcc cttttttaca gataaacaat gtgttagaag tagtatcttc ttttacataa
      121 aaagctgacc ataggataaa tggtcagcgt atttagtgag tcagctattt agtatgggtt
      181 ttctaaagga acgactgtct ctcttttgat caagcgatga gggatgacca aacgtacgcc
      241 actgctcaat tcttcatgga tcaattcgcg cagcttttgc atagcgacac gtcctaattc
      301 ggagatatcg atatcgatac ttgtaaggta agggtgtgtt aatgttgaga agatcgagtt
      361 gttgaagcta atgacggaga gtgtctctgg aatgacatag ccatacattt gcgccaactg
      421 catcgtgcga agggcgaaga tatcgtcaat gaccaccaat gctgtggctt cgttttcttt
      481 caacgttttc tcaaaggaca gataatcctc cggatgctct agtactacac tggataacgc
      541 aggaagacca gccatcatca ttgctttttg ataaccgaaa tatcgttcga aatacaaact
      601 ttcatgagtc gtgtttgtca caaacaggat attcttatgt ccattctgga tcaaaaattc
      661 tgttgcttgt ttacctagaa gctggttatc attatctaca taaacgattt cgttctcatg
      721 atgataaggc tgtccgatca aagtgaaagg gatgtggttt tggtataaat aatcaatgac
      781 agtatcttga ctatctgaat acactagaat aaaaccatct acttgttttt gtaaatgcat
      841 acgctggacg ttctctaaca gcaattcaaa atttttagca gtagcgattg ctgtagtcat
      901 atcataattc cgcgcttctt cgttgattgc ttccatgatc tcaagataaa atgggttacc
      961 gatccgctct tttgaatcaa gagggggtaa gattacgccg atggcactgg aaatgcgttt
     1021 tcctagattg cgggcagtca tgttaggaac ataacctagt tcatccataa tttttcggac
     1081 tttcttcttt gttgtctcgg aaatactagg atgatcgttg atgacacgag agacggtcga
     1141 ggtggcaaca ccggcttttt tcgccacatc ttttacggta atagccatga taaaaacacc
     1201 tttctcttgt tattgtgcag agaaccgata aatcgtctga ctgatccgtt cttgaccagg
     1261 atggaggttg atcgatccaa atttggggaa atgaacgata tcagggattt cttgcggttc
     1321 gattgctaat ccgtagtttg aatgcatctg ttttccattg acagagaaat cagcttcgaa
     1381 tccagtcgta gaaaataata ccatactttt attagaagtt gctatagtca tttggcgacc
     1441 agaatgcttc tcgaataatt gtaaggaagt tttggaaagc ctaggatgat gcaaaagaaa
     1501 tacatcatct agtccatctg gataacatgc aaggatctct tgcagtgttg gggattttct
     1561 aaagtcgatt ggcagttttt ctttttttgt ccaatttcca gtaggcagtt tttcttgatc
     1621 taattcaagc acaccatcta aaaagaccgt taactgatgt tcatagatgt ctcttgttcc
     1681 atttcctgaa aggttgaagt aggcatggtt ggtcggattg cagatggttt caacaaggct
     1741 actagcagag gttgtcatct ctaattcgtt ttttgttaaa cggtaagtga tcgtagcagt
     1801 gattggtcct ggataggtag aaaattcatc ttttaagtaa aagaccacac ctattgatga
     1861 tggagttttg aaaacttcta cttcccaaaa ttggaaagac cagcctttcg tgccaccgtg
     1921 gatatggtgt gatccgcagt ttttctccaa tctatgctca ccccaagaag catcccggat
     1981 ccttccggct acaggtccga cagttgctcc aaaaaagctt ttgtctgtta gaacatcttc
     2041 gaaacgatcg taagacaata aaacattttc tgattttcct tctttgtctg gtgcgaagat
     2101 ttggtgcagg cgggcaccgt aattgagaag aacgacttct aagtagtcgt tcttcaatgt
     2161 gattttcttt atttcagtct ctggaactgg tacgacggag atctctggat tatccatggt
     2221 catgccactt ttctttcaag taatcgatcg taagaaaagt cgtatcaggg acaagcggga
     2281 tctcctctcc aaggtcttca gcacgtccca cgccaattgg ttgagcgcca cttgagagga
     2341 tggcttggat accggcttgc gcgtcttcga tcccgataca ttcttctgct tttagtccga
     2401 cagcttttgc tgcaaggagg aagatatctg gcgcaggttt tccgtttgtt gcttctgccg
     2461 ggtcggcaat agcatcaaag taaggtgtaa gacccatttt ttcaagtagg aatggcccgt
     2521 ttttacttgc agaagcaaga gcaatcttga ttttttcttc ttttaaagaa tccagtaaag
     2581 gcacgacccc ggggaagaca tcttttggtt cgatcgtctg gatcatctct aaataataat
     2641 cgttttttct tttcgctaat tctgcaaatt cttcctttga aaaagagtgt tcttttccgc
     2701 cataagctaa aagcaaagag agtgagtctt ctcggctgac gccttttagc tgttcgttga
     2761 atgtgtggtc gatcgtaatg ccgatttcct gaccaagctt tcgccaagca cgataatgga
     2821 attcagcagt atcggtaatg acaccgtcta aatcaaacaa taccccttta aacatttgct
     2881 tcctcctttc ttagcgttat ttctagacga tccgctaaag aataagactg ttcatagacg
     2941 taaatttcta gaggagaacc ttctaataac tcgaaagtaa cttgttcatc tgtgactgag
     3001 atgaacaata aacgtccgcg ataattgata tggaaagaat aagctttcca agtttttggc
     3061 agaaatggtg cgaatctcaa ctgatcatgg gcagttttca tttgagcaaa tccttggaca
     3121 atcgacaacc agctaccagt catggaagtg atgtgcaagc cgtcttctgt gtcgttattg
     3181 taattatcca aatccaagcg agctgttcgc tggtacattt ctacagcttt gctttccatt
     3241 cctaattctg ctgctaaaat cgaatggata cttggcgaaa gggaagactc atggacagtc
     3301 attggttcat agaaatcaaa gtttttttgc ttttcttctt ttgtaaaagt atcattgaag
     3361 aaataaatcc cctgtagtac atctgcctgc ttgatgaagc aagatctaag gattttgtcc
     3421 caagaccaat gctgattcag aggaagatcg cttgtgggaa tatcggatac aggcatgaga
     3481 tctttatcta agaatgtgtc atgctggaca aagattccta attcttcgtc aactgggaag
     3541 tacatgttct ctgcgatatc tttccatttg gctagttctt cttcgctgat agtgatagca
     3601 gcttcttttt gataagttaa ataattttct cgcgtgtaac gcaagaccca agctgcgatc
     3661 gtatttgtat accaattgtt attgatattg ttctcatatt cgtttgggcc ggtcacaccg
     3721 tggatcatgt atttatttgc tcgttttgaa aaatggacac ggtctgccca gaagcgtgcg
     3781 atttctgcta aaacttccag tccttcattt ttcaaatatt ccgtatcgcc ggtgtaattc
     3841 gtataattgt agattgcata ggcaatcgcg ccatttcggt gaatctcctc gaatgtgatt
     3901 tcccactcgt tatggcattc gacaccagtg aaggtgacca taggataaag cgcgcctttt
     3961 aatccttgtt gctgagcgtt gtggattgct tgcggcagtt gattatgccg atatttcagc
     4021 agattcttag ttacttcagg tttggctaaa gcaagataaa gagggacagc atacgcttct
     4081 gtatcccagt aagtggctcc gccgtatttt tctcctgtga atcctttggg gccgatattc
     4141 aatcgttcat cttcgccgta gtaagtagag aatagttgga acaaattgaa tcgaatacct
     4201 tgttgtgctt cttcgtctcc ttcgatgacc acatcggcaa gcttccatcg ttctgcccaa
     4261 gccgctgttt gctcttcttt tgcttggata taattgtttg tctggttcgt taattctgag
     4321 acagcttttt ctgcttgctg cgcttctgga atatctctac tagtaagaac gataatttct
     4381 ttttccactg accatgtctc accttgagat aattctgcag acaagcaacc atgtaatgct
     4441 aaagtatctt cgtgatagtc agggatgact gattttccat taacgaaatg gcgcattgct
     4501 gcagtaacag taaattgttc aatcccaaag ttgtttggaa tagttttagt cgtcaaatag
     4561 ccgatttctt ctatagttcc acgatcaatc tcattccaaa aatgttcatc atagttgctg
     4621 tcttcgtttt gaacatttcc atccaatttt gatacgagtt tgactgttcc gctaccttca
     4681 agcatttcta tagttaaata gatataagct gattcttttt tgacaatgct cagaaaacgt
     4741 tcgaaggaaa tgcgtacttt attagtagaa gtagtgattg tgaatgtacg agataataca
     4801 ccatttttca tatctaattc ccagtaaaag tcttctggtt ccaaagaagc caagtcgatc
     4861 agctgatcgt tgatcgaaat atctatcgcg ataaagttca aggcgttgat cacttttcca
     4921 aagtattctg gataaccatt tttccaccag ccgacacgtg tcttatcggg ataccagaca
     4981 cccgccaaat aagtaccttg atggtggtcc cctgagtaag tttcttcaaa gttcccgcgc
     5041 atccccatat aaccgtttcc aatacttgtt aaagattctt gcagtcgaag atttttttta
     5101 tctaattgtg tcgtgctgat tttccatgga tcaatagcaa atagtcgttt gatctgtttc
     5161 atttttctcc tcctaatgag ctgttttata cgttcataat aaacgcaacc gattgcacaa
     5221 gtcaatagac aaaagaaaat atttcataga aaaaagctta aaaaaataaa tttttaggag
     5281 aaaattatag aagggaacac acgtattata ggttttatag aatggtatag acagaaaaat
     5341 aaaaaataaa gcatttacaa aaaagaaaac gcacgctata atacgtttaa cgaaaccgat
     5401 tgcgtaaagg agctaaacgg aatgaaaaag atatttagtt ttgaattttg gcaaaaattt
     5461 ggaaaggctt taatggttgt ggtagcagtt atgccggcag ctgggttgat gatcagtata
     5521 gggaaatcaa ttcccttgat caatcctgat tggacgccgc ttgtcacaac gggcggagta
     5581 attgaaaata ttggttgggc aattattggt aatcttcata ttttatttgc tttagcaata
     5641 ggaggaagtt gggcaaaaga aagagcaggc ggagcatttg cagctgggat ctcatttatt
     5701 ttaatcaatc ggatcactgg ggctatcttt ggtgtcacaa gtgacatgct tgcgaatgaa
     5761 gatgcattta cccacactct gttcggtacg aagatcatgg ttaaaggatt ttttacaagt
     5821 gtattggaag caccagcttt gaatatggga gtattcgtag ggatcatcgc tggatttgtc
     5881 ggggcaatgg cttataataa atactataat tatagaaaat tgccggatgc tttgtcattt
     5941 ttcaatggaa agcgctttgt tccgtttgtt gttatcttat ggtcaacaat cgtggcattg
     6001 attcttgctg tggtgtggcc aaacgtacag gctggaatca ataattttgg tttatggatt
     6061 gcagaatcac aagaaagcgc accgattcta gcaccgtttt tatttggaac attagaacgt
     6121 ttactattgc catttggatt gcatcatatg ctgacgattc ctatcaacta tacacagctt
     6181 ggcggatcgt atgagatttt atcaggcgca caggcgggga cacaagtatt tgggcaagat
     6241 ccgttatggt tggcttgggc cactgattta gtgaatctta aaggtgcagg ggatatgtcg
     6301 caatatgagt ttgtgttgca taactggacg ccggctcgtt tcaaagtagg acaaatgatt
     6361 ggttcttcag gtatcttgat gggaatggct tttgcgatgt atcgtaatgt cgatcctgat
     6421 aagaaagcaa gatataaatc aatgtatttt tcagcagcgt tggctgtctt tctaacaggt
     6481 gtaacagaac cattggagtt catgtttatg tttgctgctg tgccattata tgtcatttat
     6541 tcagtgatcc aaggggctgc atttgcgatg gcagatatct tgccattgcg ggtccattca
     6601 tttgggaata tcgaattgct gactcggaca ccgttagcta tcaaagcggg tctaggtgga
     6661 gatcttatca acttcgtgtt gtgtgtgatc gcttttggtg ttgttacgta tttcttagca
     6721 aatttcttga tcaaaaaatt caactttgct acaccgggac gtaacggaaa ttacgacaat
     6781 gacagtgaag aaaccgttgt atctaacagc ggaacaggaa cagcagatca gcaagtggtc
     6841 cagatcattc atttgctggg aggaaaagaa aatatcaaag atgtcgatgc ttgtatgact
     6901 cggttacgcg taagtgtaaa tgatcgagaa aaagtaggga ctgaagaagc ctggaaacga
     6961 gcaggggcga tgggattgat cgtcaaagac aatggagtcc aagcagttta tggaccaaaa
     7021 gcagatgtct tgaaatcaga tatcgaagat ctgctgcaat caggagcaga gattccagag
     7081 ccagaaatgg tttctacagg aaatggacaa gcaggtagta agcaatactt aggtatagaa
     7141 caagaactag tttccgcagc ttctggagaa gtgattcctt tatctgaagt aaaagatccg
     7201 gtattctcac aaaaaatgat gggtgacggc tttgctgtta ttccaacatc aagagaagtt
     7261 gttgcgccga tcgctggaaa agtgacaagt attttcccta gcaaacatgc aattgggctg
     7321 gaaacgaaag atggtatcga agttttgatc catatgggaa tcgatacagt ccaaatgaat
     7381 cagccagctt ttgagattgc tgtcaaagaa ggccaagaag taggaacagg aacgaaatta
     7441 gctgagatgg atcttgatgt gatccaaaac gaagaaaaag atccaacgat catgatcgta
     7501 tttacaaacg ataaagtaga agaagtagtg atcaagcaat taggtacagt gacagctggt
     7561 aaagtgatcg gctccattaa aatgtaagga gaggttgtga aaaagctgtc ctgcatcaag
     7621 tcataagaac agccaaacaa aaatagctcc tcctattttt cgtttggttg ggcttattac
     7681 cgagaagcag cagtttgaat actgtttagt ggagaggtcg tgaaaaagct gccctgcacc
     7741 aagtgataaa aacagccaaa caaaaaatag ctcctggtcc attgagctta ttactgagga
     7801 gttagcagga gaacactatt tgctggagag gttgtgacaa aaattttgtc acaacccttt
     7861 tgtttcgaaa tgaagacaag gagaaaacca atgaaattca tgacactcaa cacgcatagc
     7921 tggcttgagc cagaaccaga gaaaaaattg caagaattag cagataagat cctgttagaa
     7981 gattatgaag tgattgcttt acaagaaatc aaccaattac tggaatctga agaagtagaa
     8041 cctgcgaagc taatgaagtt ctgtccggta aagaatcaag tacctatccg taaagataat
     8101 tttgcctacc gtttagttca gcttttaaag gaacacggaa aagagtattt ttggagttgg
     8161 gaaatgagcc acatcggtta tgataaatat gaagaaggaa atgcactgct tacgaaaaaa
     8221 acattggaaa gtcaggtcct taccgtctcc caatttcaaa agaaagaaga ttatcagaca
     8281 cgcaagatct tgattgggaa aacaaagata gatgatcagg acatttttgt ctcgagctgc
     8341 cacttttctt ggtggacaga taaaaaaagt ggcttttatt ttgagtggaa aaacttggaa
     8401 aactactttt tggaaactcg cgtaccactt tttttcctag gtgatttcaa taatccagta
     8461 gactctcaag gttattatac tgtaagagaa agttgccttc ttcttcaaga ttcgtatgtt
     8521 gtcgcgaatg aaaaaggaaa agcagctact gtggaaaaga aaatagatgg ttgggaacaa
     8581 aatacagaaa aattgcgtat agattttatt ttcgtccctg aaggaatgca ggttaaaaaa
     8641 tatcaacgga tttttgatgg gatcgatagt ccaatcatca gtgatcacta cggggtggaa
     8701 atcgaattag atgtgaacga ataaaaaata taacatataa cataaaaaaa ctgattaaaa
     8761 taaaactctc caaacgatta taccgtttgg agagtttttt gatcggatgt atttatccct
     8821 tttgcctaag cacgtaaaag cttgtatcta tgataaataa ataaacatag caaggtaaac
     8881 cagccaatca aagtaagaga aagaatgaaa tagaaataga ttggcgtttg ataatgaagc
     8941 gtgagctcgt tgattccttt ttgctgcttc acgattgggg tcccgatatc tgttacttca
     9001 taatcagtca atgtttttcc attttgctgt agcacagtcc gatcgtagac gataacaggg
     9061 atatgaacag ttttgtcccc agtgtttttc cacttgattg tcaaaagacc gcctggatgt
     9121 gtttttataa aagttgtttc gttttctagt atttcttcgt tataccggtc gtatttattg
     9181 tcttttgtct ccttatagat tggcagatag tcaggtgtgg ttttttggaa aacgaacaag
     9241 ctggtagaca tatctttgct gaagaatgtt ttccttgctt cttccggcgt atcgaataga
     9301 taagtatgtc gactgacaaa tgtttcattt tcccactttt ccagatgctg gtataagttc
     9361 tggatcgtct gtgctgaaaa aataagaatg ccaaatatac ttaaaagacg gaaccacttt
     9421 tgttcagacc actggttcag tgaaagggca aggtaaagta gaagcaatag cgtaaatggc
     9481 acgaaaaatc gaaatggaaa ctggatcaaa ctaataaaag gcacattttt ttcgatcaat
     9541 gtcgtccatg gaatcaagct agtagaaaga aaaaagaaaa caagacttgt gtacaacacg
     9601 attttttgga ataaggtgtt cttttgttga aagaaaataa aaagcaaaaa acctgataat
     9661 atcaatatag gaaaggcctt agggtaatgg agccaataac tgccatccag atctatcgcc
     9721 ttttgactca tttctctgtt aacaaaggga ggaagaatct tattcccact gtaaacaata
     9781 gctaatcctg cccaaatatt tgctgtcaga acaaaataaa gcaagaccga cttaatcagt
     9841 tgaagcagtg cttttccttt ttcagtctgg ttaaaaaagt aataaagata aaaagggata
     9901 tacatcaaag ctaacatgat cgaagacagt aaatggatct gtgtcataag tcccactgag
     9961 acagcgactt tgataacagg gaacttttta tgaaggacga aatcagcaat agggatcata
    10021 cataaaggat aaaaacaagt cccccagcta gtgaatcctt ggttgataga ccagtattga
    10081 atagaatacg tactcatgaa taaaacggct actggtacac ttacccaatt tcgaacattg
    10141 acatatctca gtaaaatcag catagaaaat cctgaaataa gataaagaat gaaatttgaa
    10201 agaatctgat aatgaaacca gctacctgca agcaacacga tcaatccttg taaataagcg
    10261 aaaaatgggc cgtaaacggc attcacgatc ctgccacttt gctggaatcc ataaagggaa
    10321 ggaaaataat ggaaatcccc atatttaatt tgttgcgcgg catcatagaa ccgattgtaa
    10381 tggaatgggg catcagcacc caaaattagt ttatgtgtga ataaatgagg catgagaaat
    10441 gccaaagcaa tgaaaaaaat tgctaaaaat gggccatatt ttttcaattg tcgaactcct
    10501 ttgcaaactt tagataaatt catcatatat cctatgcgag aggtaggcaa gaaattagaa
    10561 aattcatgaa aatatttatg aattttacaa gaaaaacata agtgaaagtt ctaaggagag
    10621 aggatgcgac ataagttttg ttgcatctcc ttgttttgaa acagaatttt tcttaatact
    10681 cgaaacaaag tagctttttc atgacctctc ggtaaacggt gttcaatcag ctgcttctcg
    10741 gtaataagct cactagccta gaaaatatga gaagctattt tctgactagc gctcttatta
    10801 ctcaaagcat aacgcagttt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    10861 tgcggtaata agcccaaccc aacaaaaaat atgagtagct atttttttgg gctgtactta
    10921 ttacttgatg caggacagct ttttcacaac ctcatcggct ttcattttct atttttttcc
    10981 tgaacccata taataaaaga aagaacaaaa aaacttgcaa gggaggacac atgtgaagca
    11041 gagtaagcaa aaacagtctc gtcgactttt gtttcgtttg tttttattaa tatgtatcgt
    11101 cgtagggtta ctgatcgctt tatatccatt ttatgtggat tctttaaata gttttattga
    11161 tcaaaaacgt atcgaacaag tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa
    11221 gaaaatggag gagaaaaacc aacgattaac tgaccaagga ttcaatcctg gtactgatcc
    11281 tttcaatgaa aagaacagaa atgaaagtac aacatccagt caattagagg aatggctgat
    11341 cggaagtatc aatataccta agatccaatt aaatatttca ctttatgatc ggctcaatgg
    11401 taggatcctt gaaaatggag caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa
    11461 ttcgacgcat tcagtgatct ctgctcacag cggtttacct aatcgacggt tatttaccga
    11521 attagatcgt ttagaaaacg gagacacgtt tattttgact gttttggggg agaaattggc
    11581 ttatcaagtt aaaaatatac aagtggtact gccagatgac acgtctgttc taacgatttc
    11641 agaaggagaa gacttagtta ccttgcttac atgtacgccg tatatgatca acacgcatcg
    11701 tttgctagtg acaggacatc ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa
    11761 aggaaatcag gaaagaacca tgcggcaatg gtttattctt gcaggaacga taatagctgt
    11821 agtcatactg ttagtgttta tcggacgact aatctatcag tatagattaa gtaaaaaagt
    11881 gcttgatttt tcatttatta ttagtgattc gtcagggaaa ccggtaaatg gtagttcatt
    11941 cattttgaaa cataagaaaa aaacacttac tagaaatggt gtgccgtttt cagtacaaag
    12001 cgaccatcat gggaaagtca agctggatca attgcctggt ggaacttatc gaatcgtttc
    12061 agctgatgat cctaaagttg ctgtttcttt tggtattaga aaagtaaaac aagagaagat
    12121 atactttttt gaggggcgaa aacttgtaaa agagcttcaa aaaaatggtt tttattttaa
    12181 aatcaatgaa taagtaagga gagaaaaaat gatccgcaca acaacaaaat tgtctgccaa
    12241 agaactggaa tctgttctat cgatttggct gcatgctaac ttggattctc atgaattcat
    12301 tccatcaact tattgggaga atcaagtaga atctgttagg aaagctttac cagatgcagt
    12361 gatttactta gcagaaatag atcaagaaat tattggtttt gctggggtga ttgatcagtt
    12421 tatcgcagga atctttgtga aaagggaata ccggcaacaa agaatcggta cgcaactaat
    12481 gaaaaaaata aaagaaacca ataaaacact gactttgaca gtctatcaaa aaaatcaatc
    12541 agctattcat ttctatcttt cacaaggttt tacaattcaa acacagcaaa aagatgaagc
    12601 agtaaatgag gaggaacttg tgatgaaatg gaatcaaaac gctgattttt gatgaacaga
    12661 ataacgaaat aatgctccca gcctaatttt ttgctgggag cattatttca ttttgaagtc
    12721 actaatttaa ctgtgtgaat atgcagttgt tacaatcttt gataaacggt gttcaatcag
    12781 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt tccatttgtt
    12841 tctctaatac tcaggtcaaa acgctgattt cacaacctct tgataaacgg tgttcaaacg
    12901 gctgcttctc ggtaataagc ccactagcct agaaaatatg aaaagctatt ttctaactag
    12961 cgttcttatt actcaaagca taacgctgat ttcacaacct cttgataaac ggtgttcaat
    13021 cagctgaccc tcggtattag ctcaaaaaac ggcaaaacat gaggaactat ttttccgttt
    13081 ctttctctaa tactcaggtc agaacgctga tttcacaacc tctctttcac aaccttattt
    13141 tactcgttta taattttcat cgaagtattt tccgctacgg atatcatctg gcattccatc
    13201 gtatggttct tcgctaataa catcttcatt gtctatacct gcatcaccca tataagtgat
    13261 cgtagcaaat tcaggaacaa gtaattttgg ataagtttca tatttttcac gagctgcttc
    13321 catttctgcg atatgttcgt tttggaaagt aacagtgatt tctggatgtt cttcaaccca
    13381 tttagggaaa aagatcattc cgtgaagttt cttttcattt ggcataaagt ccaatgccac
    13441 gtctgtccct gttggctctg tccaagtgat tttgaatacg ccgtccgtca atttaacgat
    13501 atcagcttct tggtctttta cccaacggcc tttgaccatt ccgccatgga tacggtaatc
    13561 aacagtatgg tcatttttgg cataccattc atattcccaa ccattgtcat acgtatagat
    13621 aaagtgagtt ccaataaaat cttccaattc tttaaattct ttcatgttta agcatctcct
    13681 tataaattaa aacatgtaat aaactacatg ttttcgatta ctcatttatg atagaatgga
    13741 tttaaagatt tgtcaaggag ggaaaactga tggcaagaaa aaatacactt cgatatattt
    13801 tgttagggtt attaagtaag aagaagatga caggatatga attgaatcaa tcgttcaaaa
    13861 atgaaatagg agaattttgg caagcaaaac atagtcagat ttatcctgaa cttgcaaaaa
    13921 tggaagagca aggaatcatt cagcatcaag tagagatcac aggtgaaaaa ttggaaaaga
    13981 aagtttatga attaacagaa gaagggaagg aacaattggc agagtggatt catacaccca
    14041 ccaatgaact accggttaat cgagacgaat ttgttctgaa attgtatttc gtcaaagacg
    14101 tgaatgatcc ggcattagta gaaattattc agcagcagcg tgaccttcat gaagaaaaac
    14161 gacttcatct attagagcga aaaaaaacga tctttcctac tgaaaaagaa aaacatgaaa
    14221 attacggaca ctacctgatt ttagcgcatg cgatcaatcg ggaaacagaa tatgcaaatt
    14281 ggctaacaga tgtattagaa gaaagtaacc gaaaaaaata aaaaaatatc cgaacagtga
    14341 tggagaattc ctttctatat gaaagaaacg cagatcaccg ttcggatatt cttatgttca
    14401 aacaggcctt ttttcaagta tttatgtagc atctacttat taaaaagctt ggtacttagg
    14461 agattagtgg cgtttagagt tcttttaaca actgttcgta gtcagctttt tcatcgacag
    14521 gaaggagctt gtaagttaat tcgtctgtct cgttgtcatg aatgatgtac atcttagcat
    14581 tctcttcttt gaagttaccg atttgtaaaa tcgtatagcg ttcgtcgatc aattcgatat
    14641 ctttgattgc ttttgaaaaa actttacgga catggtgcat tttcttttgg cggatttctg
    14701 ccttttctgc agcttttgac aaagtaaatc cgtcatctaa atattttttg atcagttcta
    14761 cttttatgac tgtaggtaaa cgatattttc taggacaatt cgcatcagct tcgatcgaac
    14821 gaatgaatcc cttttgttcc caatacctta attgacgtgg agacacacct acgatatcac
    14881 ttaattcact gatgccaaca accaattgat cattttccaa caaacgttta agggaagccc
    14941 caaccatttt tgtcactcct tttgtcctct tgcttccctt aattatatcc gtattaactt
    15001 atgtgtcaag taggttgaca aatagcgaat ttaggactaa agtattctag tagtttcaga
    15061 aaaaattcag aaagaaggag aaatatgagt aacaaccaac ccgtagatat ttacggaaaa
    15121 ccatataatc ggacactttt agttgttgtc ttattaattg gtacattttg taccgttttg
    15181 aatcagacct tattgacgac agcttttcct acattgatga aagctttcga tatttcagcg
    15241 tctgatgtgc aatggttaac gactggtttc ttgctagtca acgggatcat gatcccaata
    15301 acagcatggc tgatcaataa attcagttca agaaatttat atctttctgc catgacgatc
    15361 tttttgatcg gaacgattac ttgctttaca gcacctaatt ttagtacatt actaatcggt
    15421 cgtttgatcc aagcttgcgg tgtgggagta tcgatgccat tattgcaaaa tatcatgcta
    15481 tctattttcc cgccagaaaa aagaggatca gcaatgggga tgtcagggat cgttattgga
    15541 cttgctcctg ctttaggccc tacattatct ggttatatca ttgataacta ccattggcgt
    15601 gacttgtttg gtatggtact gccaatcgtt gttcttgtct tggttttagc attcttccta
    15661 atgaaaagtg ttattccatt gtcaaatcct agcattgata ttttatccgc tattttatca
    15721 acgattggat tcggtagttt actttacgga ttttctagtg taggagatga cggctgggga
    15781 agcttgaaag taattggttt cttagttgtt ggtgtgattt tcatcgcctt gttctcatgg
    15841 cgtcaattac atttggaaca tccattcttg gaacttcgtg tattcaaatc atctacattc
    15901 acgattgcag ctgttttagc tggtgtaaca aatatggcga tggtaggggc agaaatggtc
    15961 gtaccattgt atattcaaaa tattcgtgga gaatcagcat tccattctgg cttgatgttg
    16021 ttgccgggtg cattgatcat gggggctatg atgcctgtga cgggtgcgat tttcgataaa
    16081 tacggcgcga aacgtttagc catcacaggg atgttcattt tgacagcggc aactgcacca
    16141 tttgctttct tgacaaaaac gactccagtt ttatatatcg tgatccttta tgcgatccgt
    16201 atgtttggga tctccatggt catgatgccg gtaacgactt ctggtatgaa tgcattgcca
    16261 aataacttga tcagtcatgg gacagcggta aataatacat tccgtcaaat cgcaagctcg
    16321 attggtacag ctattttgat cagtgtgttg acgaatgtga caaaagcaaa tatcccaagc
    16381 aaatctgtat tggataatac gccattagct tataaagatc aggcaatcaa tgccacactg
    16441 tcaggttacc atgccgcgtt tatcgtagcg atcatctttg ggattcttgg ctttattatt
    16501 gcctttttat tgaagaaaaa agaacctgtt ctaatgaaag cgggggataa tgcatgatta
    16561 ttggagtttt gattgtcagc ttgcttgctt ttgctttaac gaacgtgttc gctaaaaaaa
    16621 catggcaaac ctttttatct ttgattttcg gactaatctt cgtcgcttca ctaagtttga
    16681 tcgtcgctaa tttgtccaat cactttggaa tggaaaaagt aacggaaaca aaaacagaaa
    16741 aaattgtttc cagtgctgat tcccaaggag cagatatgct tttgtataaa gctttaggga
    16801 atggaaaaga aaaagtgtat ctttaccgaa caaatgaaaa acaagaaaaa ccaaaagcaa
    16861 ctggtacgga taacgaaaca aataaagtag aaaagacaga cggcgatgct gaaaaagtga
    16921 cgaaaactac ttattgggaa tacaaaaacg acatgtataa attctggttc aatattgctg
    16981 ataacaatca cgaatatgac agccgagtga atacattcaa aatcccagag acatgggtag
    17041 aattaagtac ggaccaagca gccaagttag ctgaattagt gaagaaacag caaagtacga
    17101 tggaaagtga agcaaaagct tatgttcaag acggtatggt aaaagcaatg actgaaaatc
    17161 caaaaatgag caaagcagag caagaacaac gtacgaaagc cttagctgct gaattccaac
    17221 aacaagcatt cgcaaaatta gtgaaagaag caaaaggcga ataatatttt gaacaccatg
    17281 taggaggttg tgaaaaagct gctctgcatc aagcagtaag aacaatcaga taaaaatagc
    17341 actccatatt tttcatctga ttgggcttag tgccgtagat gcaagctttt gaacaccgtt
    17401 taaggaagag gttgtgagcc tgccgtttaa ctctgagtca caaggagcaa tttcaaaaaa
    17461 cagcttctca tgtttgttga ctattgtgac ttgtgaccga ggagttggca ggagaacacc
    17521 gtttattcga aaaaaggaac tgcgacaaga cttttgtcac agttccttct tttttttgct
    17581 atgctatact agatgtaaaa aataggagaa ataaaatgag ggaaatttat tcttttgaat
    17641 caaaaatcta tgcttccgaa gtcggcaagg gcggggcata tattattttt ccgtatgata
    17701 taagaaaaga attcggtgct ggaagagtta aagtccaagc aacttttgac aatgtgccgt
    17761 atgaaggcag tatcgtgaat atgggcgtga aaaatccaga tggttcagtc tgctacatcc
    17821 ttggactacg aaaagatatt cgtaaaaaca tcggaaaaga aatcggtgat attgtagcag
    17881 ttacagtgaa gcaaaaataa aagaggttgt gacaaaagac gtgtcacaac ctcttgataa
    17941 acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaacc
    18001 catgtttaca aaatttttct tactactcga aacgaagcgg ctttatcaca acctcttgat
    18061 aaacggcgtt caatcagctg tccctcggta ttagctcaaa aacggcaaaa tatgagaagc
    18121 tatttttcca tttttttctc tcatactcag gtcataacgc tgatttcaca acctcttctt
    18181 tcataatggt ttttcaccat ctttataata aactggttct ttgatatgga aatctatttt
    18241 ttccaaccaa tctaaaatct tgtaacctaa ctcttctgac atcggaaaag aggcgttgat
    18301 ttgtgcattg gagatatggt caacatggat accggaagtg atcgtacaac ttcccggaac
    18361 ggcagttttg atgatcacag ctattttttc ggctaagaca tcgtctttat ggaaccgacc
    18421 atcgtggcta gggaaacgta cagtttgcat ctctgtatca cgagaaaatg tagtgactgt
    18481 tccgatatgt ggtgtatctc cgcctgttaa agtgatcaat aagtctttac cgattacttt
    18541 aacagcagct tggatcgtga aacctagttt ggttacttca actatcgttt ctttttccat
    18601 attaatcacc ctgccaacgt tttgaatgct catttacaat ttcaaattga tccaaaagtt
    18661 tcattgtatg gtgattaaca agatcttgga tcgtttgagg ctgattataa aaagcaggaa
    18721 ttggtggtat gatttgcaca cctagtcgtg ctaatttagt caagttttcc aagtgaatgg
    18781 catttaatgg cgtttcacga ggaacaatga ctaactttcg ttgctccttc attgttacat
    18841 cagcagcacg tgcaatcagg ttgtcgccta aaccaacaga gatacctgcg actgttttca
    18901 tacttgccgg tacgatgatc attccatcgg tcaaaaatga tccgctggcg acagcagcac
    18961 ctaaatcttt gtttgaataa tggtaatcaa acaggctttc gaattcagtt agtgataagt
    19021 cggattccag ctttaagttc tgttttgccc atggactgat gattccgtgt gtttctatca
    19081 tcggatattc ttttagtttc ttcactaggt tgatggcata aatggtgcct gaagcgccac
    19141 ttacaccaat cacgattttt ttcttccgtt gtgccaattg ttccattttc cgcactcctt
    19201 tttatgatgt caaaacagtt gcagatcatt cattatttca aatatttttc ccaatcttct
    19261 acttcttgga attttgcacg tacaaaatgt tctttcatat cgaaaggaac tgtaccgtca
    19321 aagatagttt ttgcacttat cccacggaaa cgaatagagc caggattata ttctggtaat
    19381 tctgatgggt ccaatggatg gttcctcatt cctgggatca cgatgatatc tttatctcct
    19441 tggaaacgag tgttgaaagt ccaaactaca tcgttcatgt cgaaaatatc aacatcttca
    19501 tcgactaaaa tgactgtctt caattctttg aaggctgata aagcaagaat agctgcttga
    19561 cgctgaatac cttcatccgc ttcgttttct ttacggattt gcatgatggt catcaattta
    19621 ccaccacctg caggaggatt gtagacattt actacttttc ctggaatagc tttatcgacc
    19681 aaggcaagaa tacttgcttc tgtaggaata ccggccatag atacgtgttc ttcactggga
    19741 ccaatcgttg tttgcatgat tggattgtct tttcggtgag taactgcttt gactttcact
    19801 acttgtacag ctggattagc atccccagta tagccaggaa attctggcat tgcttttcct
    19861 gagttcgtgt tgatatcttc ttgcattgtt tcatttggca tgatttctgc ttcaatgatg
    19921 aattcagcac gagcaatacc cacttcatcg actgctatac catcaacgat ttgaataggt
    19981 tcgttacgca aagctcctgc acaccatagt tcattgtagc caagtggagt ggtaggtggt
    20041 tcgaaagtcg tgccaatggc aattgctgga tctaaaccaa tattgatagt gataggcatt
    20101 ggtttattca aggcttcgaa ttgtttttgg aagtgaccaa tgtggcgtcc gccaggcatg
    20161 atgtacatac cgatcgtatc tttatcttct agaaccatac gatggatcgt tacatcgctc
    20221 attgtttttt ctggatctga tcctaagact acacccattg tgatgtatgg accagcatct
    20281 tcaggagtat ttgtaggtgc aggcaaaatg ttgcgaatat cgaagtctgc atcggtagct
    20341 agatgaacga cttcttgtgc tggagcgtct gctttgtcta ctttcacagg tttgattgga
    20401 ttttctacag cgtctttcaa gaaacgacca agtgttcgat aatcatggtg caaaatttca
    20461 cctacacgtt tacgactagc cattgttccg atgttcacac gtgtgccggg gaaacctttg
    20521 atattgttga aagtcatagc tggtccttct tgagtcggac gttttactgt accgccagca
    20581 ccgatataac ggtaaacacc agataattct gcttcaggat cgatttcaat atctgtttcg
    20641 tgatattgac ctggaagtgt ttttagttct tcgatgactt ttcttaaatc atacggttga
    20701 tctgtcatga gagattcctc cttagttgtc tttacctatc tatagtaaaa ggtgaaaaag
    20761 aggaaaacaa ttcaacttgt tcggtaaagt attctacaat ggaatggatg cgtttttttg
    20821 ataatagaat aaactatgat atacttatca ctaaaagaaa gcgcatactt agtgttggag
    20881 gaaggaaagt gaaaaaaatg gaggagtcat ttcctaaagc agtaaaagtg gaaaatatag
    20941 cgaatatttt gaaagttaca tttgaaaacg gagaagtaaa atacgtgaaa tctcattgga
    21001 cagaagagat aaccgatgcc ttgcaatttg gaaaaaaagg aagaggaaaa agaaaaaatc
    21061 ttctcgcact ttctcgtaat atgtggattg ggactgaagt aacgattgaa gcagatggaa
    21121 cagtattcat aaatggaaaa gacaggtata cgccagaaga gttgtggtac aaaggaaaga
    21181 aaagtatacc agaattataa aaaataatta aaaaaaacag cgcgaaacaa ggaacatatt
    21241 acttcctcgt ttcttgctgt tttctactat ttcagcgctc ttatgactca aatctaaatg
    21301 tggttgtcac aaccttattc tgcacgcaaa gcgatagcgg cgtctttttt cgctgccatt
    21361 cgtgcaggaa gatgaccgcc aagcatcgtc aatatagtag agacgatcac tagaataatc
    21421 gcatggacag gatttagttg agcaacattt tttagatccg tcatgttgta taagatgcta
    21481 ttgataggga acgtagctag ccatgcaata aacacaccta atatcccaga agaaatacct
    21541 aaaatacacg tttctgcgtc aaatacgcga gtgatgtctt ttttacgtgc acctaaagct
    21601 tttagtacac cgatttcttt tgtccgttca atcacagatg tataagtaat gatactgatc
    21661 atgatcatac ttgttactaa agagatccct gcaaaagcaa taaggacata agtgatagca
    21721 tccatcaatc cgccagtcag ttcagtcatc gttccagcta aatcagtata aatgatttta
    21781 tcttcatctg atttgccttt attgtaatca tctagataat ccaagatctt ttctttgtct
    21841 tcaaaattgt tcggataaat catgatactt gaaggcaagc tgtctccacc taaataacta
    21901 agcaatgttt gtttggcatt ttcgtcaact ttttcagtag ttaagacatt tacaccactg
    21961 tctctttgag ctttgacgat ttctgattct ttgttttcgt tgacgatttg agtcgttaat
    22021 tgatcgctat aagcgattcc tggagataag agattcattg tagaagaaga tttcacgcgt
    22081 aggatccccg agatcttcaa ttcatcagaa gcattctgat acatcgcatc ataatctgta
    22141 ttagggataa aattcccagt tggaagtttt gtatagtaag tattgttgtt tactaatttg
    22201 aatgttgtac caacgatatc atcaaaatca agtttttcgt cttctttcac atcaaatcca
    22261 agattcttca atgcgttgat attagtatta ttattgccgt ctacgatcag cacgacatct
    22321 gttgctgagg ctggataact tccggcaagt aaagaataat tgtcttttaa aaagttttct
    22381 ttgctagtgt caagctgtgt agggaaactt gacactccca cacctgtcat cgctgacatc
    22441 gtactagata aagaaagaga ttccgtatca ggattttgat tcgagaagct gacaggctgt
    22501 actttgccat ttacatcacg taaaagattg attcctgtag tacgggtaaa tccgatatta
    22561 ttacttaagt taggatcgat gtctgttacg taatcgacgt atgtttgatc aatcttattc
    22621 gtgtgctgtg ctttatcttc ttcgctgact tttgcagtaa tcgttttgct gtctggataa
    22681 tccgctttgc ttgcacccaa tccggcatcg tcacgagtct ggttagtcgt taccttagaa
    22741 atagtaatcg gaaatttcgc catcgtttca gcttgcgtgt tatcgatttg tttttggaat
    22801 ccagaagaaa gggacagaac gatcgcgatt ccgatgatcc caatactgga agcaaaggag
    22861 gtcaagaaag tacgtccttt ttttgttcgg atattgttga aggaaagctt taacgctgtc
    22921 caaaaactca tctttgttct gcgtaagtta aattgatcat cttttggtcg ttcgatgtga
    22981 ggatgggaat ctgttaagat cttaccatca gaaaattcaa tgattcgatc tgcgtattga
    23041 tgggccagtt caggattatg tgtgaccatg atcactaatt tttctttcga taactcttgg
    23101 atcaattcca tgatctggat acttgtttct gtatctaacg ctccggttgg ttcatcgcac
    23161 aaaaggatat ctgggtcatt ggctagtgct cgagcgatgg ccactcgctg catttgtcca
    23221 ccagataact gatttggctt tttatgcata tgatttgtca agccgactcg gtgcaatgca
    23281 tcttctgccc gttttcgttt ttcgtcttta gaaacaccac ttaatgtcat cccaagttca
    23341 acgttttcaa taatgcctag atgaccaatc aagttgtaac tttgaaaaat gaaaccgata
    23401 gagttgtttc ggtaggcatc ccagtcactg tccttaaagt ctttagtaga ctttccgttg
    23461 attaccatat caccggaatc ataattatct agtccaccaa tcacattcag catagtcgtt
    23521 ttaccagaac cactaggacc taaaatcgct acgaattctt tttgtctgaa agcgacagac
    23581 acaccatcca atgctttggt ggtggtttcg ccgactttat aatatttttt tatatctttt
    23641 aattgaagca taatcaccct tctcccgtat tgaaatattc ggtatactaa taagtatatc
    23701 agaacaatga tcgtagtata cgaaataaat gttaactgaa gatgaactac aagaaaaaat
    23761 ctagaaattt ttcaaaaaac agagggagat caatgaatcg tattttagta gtagaagatg
    23821 atccgcaatt acagttgctt taccgttctg tgttggaacg agcgggcttt tcagtcatca
    23881 ccgcattgaa tggagaggat gcgctgaaaa aactagaagc gacacgtata gaattaatca
    23941 ttacagatat catgatgccg aaaatggatg gctatgactt gctagactca ttacgtagca
    24001 ccagatttga cacacctgtt ctaatgatta cggcaaaggc cgattttgag gataagaaac
    24061 gcgggttcaa attaggagca gatgattata tgacaaaacc catcgatgtc aatgagatga
    24121 tcctacgtgt ggaagcattg cttcgacgtt caaagatcaa ttacagcagt agcttgataa
    24181 tcggacaaac aaaattagag ctagatgctt atacagtgtt acaagatcag cattcgttga
    24241 ttctgcctca aaaagaattt ctgcttctgt ataaattact gtcttatccc aacaagatct
    24301 tcactcgcca acagttgatg gatgatattt ggggattaga tacgaataca gaagaacgaa
    24361 cgatcgatgt acatatcaaa cgtttaagga cccgttttga aaagacggaa gattttcaaa
    24421 tcatcacagt acggggacta ggctataagg cggttattta aagatgaaaa aaaagctatt
    24481 ttcacaatta tggatctatt ttgctagtat tgtcttcatt tcgatcttag tgacactgct
    24541 gtgttttctc ggttttattt tcttattgtc tcgacattct atctcgcctg ctgaatcggc
    24601 tggaaaacca acgttatacc cgttattcgt ctttgcgggg ttcagtatgg tcgtgggtac
    24661 aggaatatcg atttttgttg ggcggcgtat cctgcatcca atcagtgcgc tagggacaaa
    24721 tatgagtctg gtagcaacgg gagatttttc gattcgaatg gatgaacagc agaaagtagc
    24781 agaagtacag caactgtata aagatttcaa cgttatggtc caagaactca atagtatcga
    24841 gacactgagg aatgactttg tgtccagtgt atcccatgaa ttcaaaacac cactagcaac
    24901 aatccaagga tatgttcaat tgttacaagc cccaaatctc tctgacgaag aacgccaaat
    24961 atttttatat cggattatcg aaagtatcac gcagctttca caattaacag agaacatctt
    25021 aaaattgaac aaactggaaa accagcgtat ccaactggaa aagaaagagt atcgattaga
    25081 tgaacaaatc agagaagtaa tcgttttttt tcagcctaaa tgggagaaag aacagctaga
    25141 attggatatc gaacttgctg cagtaaatta cacaggcaat gaagagtttt tgtaccaagt
    25201 gtggctgaat atcatggata acgccataaa gtacaatcaa atcaatggac agattcatat
    25261 caaactattt gagacagcca cagagattgt actggaagtg accgattcag gtgtaggaat
    25321 gaagagacca gagatcgaat gtttgaaaaa ttttatcaag gagatacaag tcgtcaaatt
    25381 tctgggaatg ggctaggact ttcactggtt aaaaaaattc tagaactgca tgatggacgt
    25441 attgattata gcagtatcga aggagtagga acgacagcaa tgatacggtt aagtaaacaa
    25501 taggaaaaag aataaaaccc ctaaaaattc tattgaaggt ttttaggggt ttttagtcgt
    25561 ttttttgtga ttagggaaat gttcttacgg ttcttttggc ataaaaatcg ctgctttctt
    25621 attgaactac gagaaatgtt agaggtgtca ttgaaacaag gacatgatgc aagaaagaga
    25681 gcgaaagtca tgcgtcgctc tctttttttg ggtggataga tggacagaaa atgtagggct
    25741 tagttttctt cttgatttaa atactcccaa aatgtatctg aagccaaaga atgctcaccg
    25801 actttacgag taaatgcaag ataactctct gttgttggag aaatagggag aatcacagta
    25861 ttttctttta atagattctt gactgttttt tccataacaa tagcaatccc catgtcattt
    25921 gcaatcatat tgatgatcaa gtcgattctt gctcctttat aagagatttt tggttcaaat
    25981 ccagcttcgc gacacagatc tagtacaggt tgatacaagt tcgtagtggg gccaaggagc
    26041 aaaaagttct cctttttcag ttcgcttaga tccaatttct ttttactagc taaaggatga
    26101 tttttaggta gaactgctac aaaatcgtct gtttcagtag gcaaccactc aatcgaactt
    26161 ggtggggcct ggaaacttct agcaaagtgg atcgtgtcac tatgttcatt ggcgtcattg
    26221 taagttaatt cgacactttc cacttctttc aactggactg taaattcagg atggcgtttc
    26281 aaaaactcgg tgattttgga aaaaccttta taattgatca ttgtcggtat cgttaagata
    26341 tttaatgaaa gattgttgtc tttcgtatag gtttcaagca cttcttgcat agcatggtat
    26401 tgttctacga cattttttac atatgggagc aataagcttc cagcttcggt caattcgatt
    26461 tttctatggg cgcgttcgaa taatttgacg tctaattctg tttctaacga aagaatctgt
    26521 ttagaaatat ttccttgggt ggtgaattgt cgatcagcag ttttggtgaa attcattgtt
    26581 tcggctaaat caagaaaaac ttcgaattta tggatatcca tcatattcct cctacttatt
    26641 ccaataaaga atactttaac acaaaaaatg aattggtgta atcttctctg tctgttacaa
    26701 tctttcttgc agcgaaagca ataattacag aaatttttct gatttttttc gcttcgtaga
    26761 aaaagagagg agttttttga tgccaactgg agttttgatc aatgtaggtt ccgttttgtt
    26821 tggcggacta attggcggat tagtaggaaa taaattaagt gaacatttta aagcacagct
    26881 gacgatggtt tttggtgtgt gttcgatggg gatgggaatc tattccattg cgccgatgaa
    26941 aaatatgccg gctgttattt ttgctttagt gataggaaca gcgattggtt tgattgttca
    27001 tttaggtaat gggataaata agggggctgc gctgatgcaa gtacctattt ctaaaatctt
    27061 ccctagtgaa aagcttggta tgacccacga tgaatttatc agcacattag tgacggtcat
    27121 tgttctattt tgtgcaagtg gtacggggat ctatggaagt ttagattctg ggatgacagg
    27181 agacagtacg attttgattt ctaaatctgt ccttgatttt ttcacagcag ctatttttgc
    27241 gtgtaattta gggtatgtcg tttctgtgat agcaattccc caattcatta ttttttatat
    27301 tttgttctta ttggcaaaat ttatttatcc attgactaca ccagacatga tcttagattt
    27361 taaagcttgc ggcggattct tgatggtcgc taccggtttt agaatgatca acgtgaaaat
    27421 gttcccagta gctgacatga tacctgcaat gatcgttatc atgccactga gctggatgtg
    27481 gacaaattgg attatgccac tgttataata aaaatcaaag agaagccatt ttttgctgac
    27541 taaaaaggat caaatctttt agtcagtttt tttgtatttg acaaactgat tttgaaacta
    27601 tattatatgt tgtataatat agattgaaat aaaatattac acttgagaat agaaggtgac
    27661 gtaatgcaca tccagattcc aactgttctt cttgatggta ctgtgttagc agcattaagc
    27721 aaggaagata tgtatgggta tgccttaaca aaaatcgtac aagaaagtct ctctgtcagt
    27781 gaatctacac tatatccggt tttgagaagg ttgaaaaaag aaggatatct tgagacctat
    27841 gatgaacctt ttgaaggcag aaaccgtcga tattactcgt tgacaccaat gggaagtcgt
    27901 catttagaag aaattattga tgaatggatt gtttttcgag attctttaga ccggttattg
    27961 gaggagagca caaatgaatg aatatttgaa tgaagttgtg gatcatcttc aagaaattcc
    28021 tgaagacgat cgttttgatt tgatccagta ttatgaggaa tattttttag attctggaaa
    28081 aacattagat cagatcattg atgaatatgg tacaccaaag cagtttgcct taaaattaaa
    28141 aatcagttac ttttcagaag aagaacaagc agaaaacgag ttagtgaaaa atccaaaaag
    28201 acaaattcgc ctgatttggt taatcattat cggtttattt gcttctcctg tcctcattcc
    28261 actagcactg gggtttatat tggttattgg gggactattg gttgctttac tagcgctcat
    28321 ttttagtatc tatgttttat gtatcagtat cttaggtgtt ggcctattca tgatcatctc
    28381 aggaatcagt gtgtttggac aatcgattct gagtggatta ttctttatcg gtgtcggtct
    28441 gttcgccaca ggaagcgcag tatttatttc gccactgttg ctgaaaggaa caaaagggct
    28501 tttttatgtc atgatgaact ttgttaaatg gatgggacga cgttttatta caaaaagaga
    28561 tctgcattca gcgggcgttt gaaggaggac acataaattg agattgaaac attttttgat
    28621 taccggtatt ggtctgatgc ttactggtgg cctcctagca ggtgcaactt atacattagg
    28681 cgctcaaaag agtctgactt gggataatgg tcctcggttg atcgatatgg ttactgagac
    28741 aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca gaaaatcaaa cagtttcggt
    28801 catacgcgga ttggattttg agataaaaac gacttatgat aagacagaaa agccagaaat
    28861 aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa aaatcaacag gtgcaatcat
    28921 tagtttagaa gacacaagtg cacaaatcac tattgttgtc cctgcagatc atgaattatc
    28981 tgaattgaca atcgaaggga ataatagtgc aattgatatc aacgaagtat caatcaaaaa
    29041 agtgaatact aagacacatg atggctgggt atcgttcaat gaatttacag gaggaacaat
    29101 aactgcgaaa gatgaattag gctcacttcg actagatgag gccaatgtgg ataacttgat
    29161 cgtcacaggt gaaaacagca acttccatct agaagacagt ttgatcagca aagaaggaaa
    29221 agtcatccta aacggtggta atttatcttt gcatgagatg gaaaacagtg gctatactat
    29281 ccaaacgtct ggtgaatctt atattgaaaa agactaccag ccaataacaa atactttgtt
    29341 agaacaagga cagaaaaagc taaagatcac tgctaaagac acatcgatca atattacgac
    29401 ggatcaagag gacaatgaaa cagattgatc ctgtactcaa ctggatattt taagaaaaaa
    29461 cagcaaagat ccttccaatc tttgctgttt ttcatcgctt aacgcacata actatagtaa
    29521 aataagaaag aaaatatcta aaaagaaaac agggtgaaag agtatatgaa ggtagaagaa
    29581 ggtcaaagag aaaagttaaa gacagaaatg gaaagattac atacttatat cactcaacta
    29641 agccaaacct tctatgatcc agacaaagaa aaagtgatgg ttaattaccc gaacaattca
    29701 gaaggtcgcc aattagagca agtgtaccat gaagttttca aacatttatt gacggtgaag
    29761 aaagagctgg attattattc tttaccgatt atcgatacag gaatattgaa gtatgatggg
    29821 aagaaggaac gatttatctt taaatctgtt cgggaagatt tgcctctttc agcagggatg
    29881 gatctagagg tcttggtaga agactatttt acagaagaaa agcactgggt tcgaacaaaa
    29941 ttggattatt taccgcaggc agccggaggg acacaggcaa gcggttggta catcactgaa
    30001 gacaaagagt tagaactaga aggtgtaatg gcgcgcattc gaaaaaaaat taaaaaagta
    30061 acattagata tttattgaag gataaaggag cgcaggaagt agcatgagag aaaaaagagc
    30121 attttatatc aatggttatg cagggattgt cggattggtc gtattagctt tagttggatt
    30181 gtttttcttc tatttaggca tgtggcaagc gaaagtttgg gcactttttt taagtatttt
    30241 tctttggttg atcacgttat tgttgttaag ttcggcaact gtagtcagtc ctaatcaagc
    30301 aaaagtaatt ttgttttttg gacaatattt aggtacgatt cgagaaaatg gctttttcct
    30361 tacgatacct ttggcacaaa aaatgactgt ttcgttgaaa gttcggaatt tcaatagttc
    30421 tgttttaaaa gtcaatgatt tggatggaaa ccccatcgaa atctcagcag tagtagtctt
    30481 taaagtcgtt gatacagcaa aggcattatt cgatgtagcg tattatcaag attttgtaga
    30541 aatccaaagt gagacagcca ttcggcatat cgctagccaa tacccgtatg atacattcaa
    30601 tgagaatgat ttgacgcttc gaggaaacac gacggcagtg tctgacgagt tgcaaaaaga
    30661 gctgcaagag cgtttagcag tagcaggtgt cgaagtaatc gaaacacgct tgaaccattt
    30721 agcctatgca acagaaatcg ctagtgccat gctccaacgt caacaagcaa aagccatttt
    30781 gtctgctcga caaaccattg ttgaaggagc tgtcacaatc actcaaatgg ccttagagca
    30841 aatcgaagat ggacaagaaa tcaatttcac cgatgatcgg aaagtccaat tgatcaataa
    30901 tttacttgtc tcaatcatta cagacaaagg aacgcaacct gttatcaata caggcgacat
    30961 gaaagaccgt aagtgacata ctttttgtaa gataggaaga aggagtttag gacggattat
    31021 tgtcttgaac tcttttttta gttgttttaa agaaaatgac aaaattgaaa gaagaatgat
    31081 agttgattcg aaggcagaat tgttcgcttt tttctataat cagtatataa agtaagagag
    31141 aaggggaaaa agtgaaaaaa cttctgatcg gtctacttgt tttcatcgtt gtggtacttg
    31201 caggggtgaa aatcgcagat tacgtagtta tgggtggaga agaatactat gtacaaatta
    31261 cgacagatgg tacaaaaaca gtttcaaaat cagactctgg acaagaaaca gtcaactata
    31321 gatatatcct gcctggctat acgaaagagg gtaaggaaaa agaactggac ttcaagggac
    31381 aattgactcg accgcttcgt aaaggagcat atttaaaaat cacctggaac aaaaataaag
    31441 gtgtcacttc gtatgaagaa gtcgaaaaaa aagacgttcc gaaaacagca ctggaaaaac
    31501 taattaaagg gtagggaaag aaatgaacaa aatagtcgaa gtacagaatg taacaaaagt
    31561 atacggaaaa aacaatgaga aaaagacgca agcattaagc ggcatcagct tcgatgtaga
    31621 aaaaggcgaa tttattggga tcatgggcgc ttctggttct ggtaaatcga cacttttgaa
    31681 tattttatcc acattagata aaccaacaga tgggcatatc cgaatcaatc aaaaagatgt
    31741 gaccacatta aaaggaaatc aactagcaga ttttcgtgcg aatgagattg gctttatttt
    31801 ccaagacttc aatttattag aaagcttgac agcacaagaa aatatcgctg ttcctctttc
    31861 tttacaaggc gtcaaaccaa aagaaatcaa gcaacgtgtc caaaaaattg cagaacgttt
    31921 atccatctct catattttgg aaagctatcc ttctgagatt tctggtggac aaaaacaacg
    31981 ggtcgccgcc gcacgtgcat tgatcacaca accaacgatt ctattagggg atgagcctac
    32041 aggtgcgctg gattccaaaa gtgcccggga tcttttagat acgatggacg aattgaatac
    32101 aaaagatcat gtatcgattc ttcttgtgac gcatgatccg ttttctgcaa gttattgcca
    32161 acgtatttta ttcatcaagg atggcggtat ccatcaagaa gtgaaacgtg gagatcaaag
    32221 ccgtgattcc ttctatcgag aaatactatc tattcttgga aa
//
LOCUS       DAFEHS010000024        32113 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFEHS010000024
VERSION     DAFEHS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.024"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            1..1197
                     /locus_tag="LP327_001206"
     CDS             1..1197
                     /locus_tag="LP327_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL1725335.1"
                     /translation="MEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQPLNDELR
                     EIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDYGKYGYE
                     SKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMTAGGVMK
                     RLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTGETLYLG
                     STSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLTYRDAER
                     TAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTLQKALHW
                     LQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERIDTAVPQ
                     ENDGIF"
     gene            2186..2395
                     /locus_tag="LP327_001207"
     CDS             2186..2395
                     /locus_tag="LP327_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBL1725336.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2507..4120
                     /locus_tag="LP327_001208"
     CDS             2507..4120
                     /locus_tag="LP327_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL1725337.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            4155..5366
                     /locus_tag="LP327_001209"
                     /pseudo
     CDS             4155..5366
                     /locus_tag="LP327_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5389..6315
                     /gene="glsA"
                     /locus_tag="LP327_001210"
     CDS             5389..6315
                     /gene="glsA"
                     /locus_tag="LP327_001210"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBL1725338.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6523..7983
                     /locus_tag="LP327_001211"
     CDS             6523..7983
                     /locus_tag="LP327_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725339.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8204..9967
                     /locus_tag="LP327_001212"
     CDS             8204..9967
                     /locus_tag="LP327_001212"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBL1725340.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10051..10509
                     /locus_tag="LP327_001213"
     CDS             10051..10509
                     /locus_tag="LP327_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBL1725341.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10618..11301
                     /locus_tag="LP327_001214"
     CDS             10618..11301
                     /locus_tag="LP327_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL1725342.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11448..12062
                     /locus_tag="LP327_001215"
     CDS             11448..12062
                     /locus_tag="LP327_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBL1725343.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12078..12284
                     /locus_tag="LP327_001216"
     CDS             12078..12284
                     /locus_tag="LP327_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725344.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12825..13955
                     /locus_tag="LP327_001217"
     CDS             12825..13955
                     /locus_tag="LP327_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL1725345.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            14047..14802
                     /locus_tag="LP327_001218"
     CDS             14047..14802
                     /locus_tag="LP327_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL1725346.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            14860..16899
                     /locus_tag="LP327_001219"
     CDS             14860..16899
                     /locus_tag="LP327_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBL1725347.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(17008..18159)
                     /locus_tag="LP327_001220"
     CDS             complement(17008..18159)
                     /locus_tag="LP327_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBL1725348.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(18313..18843)
                     /locus_tag="LP327_001221"
     CDS             complement(18313..18843)
                     /locus_tag="LP327_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL1725349.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(18942..19292)
                     /locus_tag="LP327_001222"
     CDS             complement(18942..19292)
                     /locus_tag="LP327_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBL1725350.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(19449..20978)
                     /gene="ahpF"
                     /locus_tag="LP327_001223"
     CDS             complement(19449..20978)
                     /gene="ahpF"
                     /locus_tag="LP327_001223"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBL1725351.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(20988..21551)
                     /gene="ahpC"
                     /locus_tag="LP327_001224"
     CDS             complement(20988..21551)
                     /gene="ahpC"
                     /locus_tag="LP327_001224"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBL1725352.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(21695..22357)
                     /locus_tag="LP327_001225"
     CDS             complement(21695..22357)
                     /locus_tag="LP327_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL1725353.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            22541..24187
                     /locus_tag="LP327_001226"
     CDS             22541..24187
                     /locus_tag="LP327_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL1725354.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            24178..25260
                     /gene="uxuA"
                     /locus_tag="LP327_001227"
     CDS             24178..25260
                     /gene="uxuA"
                     /locus_tag="LP327_001227"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL1725355.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(25368..26921)
                     /locus_tag="LP327_001228"
                     /pseudo
     CDS             complement(25368..26921)
                     /locus_tag="LP327_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            27069..28210
                     /locus_tag="LP327_001229"
                     /pseudo
     CDS             27069..28210
                     /locus_tag="LP327_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(28336..29415)
                     /locus_tag="LP327_001230"
     CDS             complement(28336..29415)
                     /locus_tag="LP327_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL1725356.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(29518..29958)
                     /locus_tag="LP327_001231"
     CDS             complement(29518..29958)
                     /locus_tag="LP327_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL1725357.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLAAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(30015..30425)
                     /locus_tag="LP327_001232"
     CDS             complement(30015..30425)
                     /locus_tag="LP327_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL1725358.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(30605..31039)
                     /locus_tag="LP327_001233"
     CDS             complement(30605..31039)
                     /locus_tag="LP327_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL1725359.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(31139..31987)
                     /locus_tag="LP327_001234"
     CDS             complement(31139..31987)
                     /locus_tag="LP327_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL1725360.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT        10974 a         5624 c         6202 g         9313 t
ORIGIN      
        1 attgaagaac taagacaaaa gcgtgaggaa tacggagtaa cacaaacaag gcttgctgtt
       61 gcctgtggta tcagccgtga atattacaac cgcattgaaa aaggaaaaca gccattgaat
      121 gacgaattaa gagaaatcat agaaaaacag attgagcgtt tcaatccgca agaaccacta
      181 ttcttattga ttgattactt tcgtgtccgc tttcctacta cggacgcatt ggcgattatc
      241 cgtgatgtat tacaactgaa atctaattac atgctttatg aagattatgg aaaatacgga
      301 tatgaaagca aatatgtact tggcgacatc aatatcatgt gttccatgca ggagcattta
      361 ggtgttttat tggaactgaa aggcaaaggc tgtcggcaaa tggaatgtta tctgctggca
      421 caggaacgct catggtatga cttcatgctg gattgtatga cggctggtgg tgtgatgaaa
      481 cggcttgatt tggctatcaa tgataaagca ggaatattgg atattccaaa gttaaaggaa
      541 aaatacaagg ctggcgaatg tatgtcctac ttccgtatgc agaaagatta cagcggtaca
      601 gaaaaatgcg gtagcgattt accaaagaat acaggagaaa ccttatatct cggttcaaca
      661 agtagcgaat tatatatgtg tgcttatcag aaaaattatg agcagtatgt caagaatggc
      721 acagaaattg aagatacgga gattaagaac cgttttgaaa tacgcatgaa gaacgaacga
      781 gcctattatg cggttgtaga tttactgacc tatcgggacg ctgaacgcac cgctttttct
      841 atcatcaatc attatgtccg ctttgttgac agagaggacg acaagccaaa aagtcaatgg
      901 ataacaaatg atgattgggc atggtttgta ggggaaaaca gagagccgat acgcttaacc
      961 acaaagccag agccttacac tttacaaaaa gcgttgcatt ggctacaaag acaagtcgca
     1021 ccaaccataa aaatggtaca ggcattagac agagaaaatc gtacaacgat actgaaagat
     1081 atgattgagc aggcagaact aaaagacaag cataaacatt tattacaatt agagaaatca
     1141 accatagaag aacgtataga taccgctgtt ccacaagaga atgacggtat tttttaatgt
     1201 aattgaaaaa tttcagattt agtcagcaaa gagctgtttt cattccctat gtgaagtgaa
     1261 agaagaaaaa taaatttttt attaaatctg caacaaaatg gctacttgcg tacagatatg
     1321 gtgcgagaaa aggaaatcta aacttttttt gaaaataggt cattaaatcg gggcttgctg
     1381 tccctatatg aagtgaaaga agaaaaaagt tttgaaaaaa tgacggaaat tcgtgattag
     1441 tggacagtct tctgtgaaaa gaggaaaaac atcatcattt tgtattcaaa caggcattag
     1501 cagttgagtg ttgatgtgaa aagatgaaaa tactttgttc tcaacttaac aatttaaggt
     1561 ttccgttccc tatatggtgc aaagaaaata tttcattcta ttgttggcag taacggtatg
     1621 gtttgggtag ttagggtgtg aaaaggaaaa tattcttttt tcatcatcaa cttagcaaaa
     1681 tgacattttc tatccctata tggtgcatga aaagaagtta aaatttttcc atttaaactt
     1741 agcaaatgga cgtttgctgt acagatagta gtgaaaaagg agaaaatcaa ccgttgattg
     1801 ccaaaatgaa ccgttccatt tgtactaata atggaaaaag ttttggattt tggttacgtt
     1861 tctcctcttt tccaacgcga tatataggaa agacgatttt tttctttcaa tcgttctttg
     1921 acaactgaat aaagctattc aatacgtttg acagacagct agccgttgaa acaaatacgc
     1981 cacgaccttt cccctgcaag agcgagcgaa aacctatttg tgttcttgta aaagatttgt
     2041 cctcttttat cgccatgacc tgtactgtca gataatgata ctcccgtaca gccacagctt
     2101 gagcattaag agcgtcgcac gcaatgggta cggctacata agaactatgc agaggtggaa
     2161 ctcctgtggg ctatgacaaa agccgttgaa ttgcttagaa agattttaca gaaagggggt
     2221 atgtgttatt gataatgctg taaaaacaat tcaaaagaaa aatggcaagt gtggcattgg
     2281 caacagagga aagacaaaga ttgtagtaaa agagcatttt tccgaaaagg gcaaaacaat
     2341 ggaagaactt ctaactgatg tcatgctgga aaaagcaaag cagacaatcg cataaaatcg
     2401 gtgcagttgt aagaaataga ttgaatgaca aaaagtaccc gtgttatact ttacttgcaa
     2461 gcaggtattg taaacacggg ttagttataa aggaggataa ctgacaatga aacaacagat
     2521 ttacaatact gcactttatc ttaggttaag ccgagatgat gaattacagg gcgaaagttc
     2581 cagcattacc acacagagaa gtatgttgcg tttatatgca aaagaacatc atttgaatgt
     2641 cattgatgaa tatattgatg acggctggtc gggaacaaat tttgacagac cgagttttca
     2701 aagaatgatt gaggatatag aggcaggaaa aatcaactgt gttgtaacga aagacctttc
     2761 ccgtcttggc agaaattata ttatgacagg acaatataca gaattgtatt tccctagtca
     2821 taatgtccgc tacatagcga ttgatgacgg tgtagacagc gaaaaaggcg aaagtgagat
     2881 tgcaccattt aagaacatca tcaatgaatg ggtggcaaga gatacaagcc gtaaagtcaa
     2941 atcagcattt aagacaaagt ttgcggaggg ggctcattac ggtgcttatg ctccgttagg
     3001 atataagaaa caccctgaca tcaaaggaaa actgttggtt gatgatgaaa caaaatggat
     3061 tattgaaaaa atcttttccc tagcctatca aggttacgga agtgcaaaaa tcacaaagca
     3121 gttaagagca gaaaaagttc cgacagcgtc atggctaaat tttacaagat acggtacttt
     3181 tgcacatatc tttgagggaa aacccgaaag taagcgttat gagtggacga ttgctcatgt
     3241 caaggcgata ttgaaaagtg aggtttatat cggaaacagt gttcacaata tgcagtctac
     3301 ggtatcgttc aagagcaaaa agaaagtgcg taagcccgaa agtgaatggt ttcgagtaga
     3361 aaacactcac gagccgatta ttgacaagga agtgttctat cgtgtgcagg agcagataaa
     3421 atcaagacgc agacagacaa aggaaaaggc aacgccgatt tttgcagggc ttgtcaagtg
     3481 tgcggattgt ggttggtcta tgagatttgg aacgaataag acaaataaaa cgccttacag
     3541 ttattatgct tgcagttact acgggcagtt tggcaaaggt aattgttcta tgcactacat
     3601 tcgttatgat gtgctgtatc aagccgtatt ggaacgcttg cagtattggg ctaaggcagt
     3661 acagcaggac gaagaaaagg tattgaacaa gatacagaaa gctggcaatg cagagagaat
     3721 acgggaaaag aagaaaaagg caagtacatt gaagaaagcc gagaacagac aaaatgagat
     3781 tgaccgttta tttgcgaaaa tgtatgaaga tagagcctgt gagaagataa cagagcgaaa
     3841 ttttgtgatg ttgtccagca agtaccaaaa agaacagata gaactggaac agcagataac
     3901 aagcctaaga gaagaactaa gtaaaatgga acaggatatg ataggtgctg aaaagtggat
     3961 tgagttaatc aaggaatatt ccgtaccaaa ggaactgaca gcaccattat taaatgccat
     4021 gatagaaaaa atcctcatac atgaagcaac aacgaatgag gataacgaaa gaatacagga
     4081 aattgagata tattaccgat ttattggaag agttgagtaa tcaacaagag taatattttt
     4141 aactaaggga aaccggcggt gtctatactt gggtcagtcg cacattggga aaacgattcg
     4201 gttttgccgc aatctttttc caatggtttg agataacagt tggctacttg accatgctgt
     4261 atttcttaac tggtgcatta tcctatgcaa cgggtattgg tgctatacaa aacaataaat
     4321 atctgaaatt ggcaatccta ttgattattt tctgggctat tttactctca cagttaaaag
     4381 gaaccaaata cacttcattg attgctcgga tcggttttgt agctggtatc ttgttgccag
     4441 cattagtctt gtttggctta gggatccatt atgtcgcttc aggcgctcca ctacaaacga
     4501 tactgtctat gaaaacattg attcctgatt tttcgaaatt gcctacgctg gttgtttttg
     4561 tttcctttat tttagcatat atgggcgtag aaacatctgc cagtcatgca aatgaaatgg
     4621 aaaaccctaa aaaggattat ccattagcaa tgtttgtatt ggttattctg gcaatcattt
     4681 tagatacttt tggcggattg accgtcgcta ctacagtacc tcaacatggt ttgtctctta
     4741 atacaggggt gattcaatcg ttagaattcc tattaagaca cattgatcca aatcttgcat
     4801 ggattgcgaa aattcttgcg attcttgttt gtttaggtgt catcggagaa attgcttctt
     4861 gggtaacctc tccttcaaaa gcacttcacg tagcagcaga agaaggatta cttcctgaat
     4921 attttgcaaa agaaaacaca cacggtgttc ctgctcattt gatgatcgca aatgggatcg
     4981 ttgcaacaat ctgggcagcc gtctttacat taagcggcgg ggggaataat ctttcatttc
     5041 ttgcagcgat gtcattgact gttgtaatct acctgatgtt gtactttatg tttttcgcgg
     5101 gctatttgaa gctggttttt aaagatgaca gtcttgaaag agcctatcag gttccaggcg
     5161 gaaaagttgt aaaagtaatc gttgcttgtt taggtctttt gacttcactt tttgcttttt
     5221 gtatctcatt tgttccacct gctagtatcg cagctggaca gcatgggatt tatgaaacga
     5281 ttctgattgt ttcttatctt atcgtcttag ttattccatt tacgatctac gcatgccgtc
     5341 ataaattcca gcatgcagaa aaataaaaaa tgaaagctgg ttattcaaat ggaagtaaaa
     5401 gaattagaac aattgattgg aaaaaatttc cactattatc aaacaggaca agtggcggat
     5461 tacattccag cattagctga agtggatccc aaacaattag gtatggcgat ttatgatttg
     5521 gatacaaaac aactgattga gggaggagac gccgatgtgc gctttgccat cgaaagcatg
     5581 tcaaaagttc ccgtattgtt attagcaatc aaagacaacg gaatagaaaa agtctttcaa
     5641 acaatcaaca cggaaccaac cggttttgcc ttcaattcta tcatgaatat ggaaatcaat
     5701 catcgcaaac atccaatgaa tccttttgtt aatgcaggag cgattgcaac aacctcgttg
     5761 attgccggta aagatgcaga tgaaaaattt gagcgaatct tgtcatttat gaaagagatc
     5821 tgtgatgatc ctaatatcac attgaatgaa gaaatctatc attctgaatc aagaacaggt
     5881 gatatcaatc gctctcttgc ttactatatg aaaggtaatc aaatgatcga aggagacgta
     5941 ccggagatac tcgatgctta tttcagacaa tgctccctca atgtcaccgc tacaggcata
     6001 gcaaaattag ctgctgtgct ggcaaataaa ggaattgctc cttggaatgg caaacaaatc
     6061 attactgaag aaagtgctac aatcgtcaaa tcaattatga caactgctgg tttgtatgat
     6121 gaatcaggag agttttccgt ccatgttggt gtacctgcaa aaagcggtgt aggcggcggg
     6181 ttgatggcag ctgtgccaaa tcgttacggc ataggtgtct tcagcccggc actagatcca
     6241 tttggtaaca gcgcagctgg tatccaattg ttgaaagatg tagtcaaaga actagacgct
     6301 gacatctttg aataaccatt cttgaaagag gctggggtaa aagtccaaac ggctcaaagc
     6361 tacctacaat gaggctgaaa ttgtgagctt gtttgacttt tgtcccagtt tcttttttta
     6421 ttggtttttg aaagggcttt tctaaataaa aaggaaacaa aaatacgatt tacagatatg
     6481 attaaataaa atcataagaa aaactggagg cgtgtatcag gaatgctgga aaattacatc
     6541 gagcgaaata tatttagaaa agtgtatctg tgtgaacaat tatttgaatt ccaagaaata
     6601 gacatcgaac aaacagctat ttctctaagg gtgactacac caacgatcct tcacgatcta
     6661 gaaagtctag ccgagtgtct agaatattgt ataaaagaac aagtaagaga gaagcataag
     6721 tataaactcg tattcaaaca tggtattgct ctttctgagc tcacacagtt tctgtacggt
     6781 caatcttatt ttttaaagtt tttaagttat tattttaatg gtatattcac gagtacagaa
     6841 ctggctgatc ttgaatttat ttctttgagt aaagtatata caatcaaaaa aatcgtattg
     6901 gattttttta aagaaaatga ttatttgaaa aataaagaaa tcattatccc agaatttgac
     6961 tcgcggaaca tcttgctagc tttagtacgt tatgtcaatt gggaaggcta tgaaaataaa
     7021 aatccgtatg tagaagaagc ttgtaacgag ttgattgatt atgtagagac tcattttttt
     7081 aaaagaagat attcggaaga tgaaaagtcc tttatcatac gagaaataga gatcgcaatc
     7141 ggaagaaagg catttcccct tggactgaaa gaagaagata agaaaagcgc agaaacgaag
     7201 ccgttatttc aattagtctg tcaaggattg gaagagcaaa aaggaattct agacttacaa
     7261 gaagaagatg tctattatat tttttctttg ttcaacagta gaaattatac caatgagaac
     7321 atggagctgt taagaaaaga tttcgaagtt gtttataaaa attttgttct caatgaccta
     7381 tcactgaatg aattgatttt acagattatt agtatcgctg aaatcgacga tgcagaaaat
     7441 tttattttca agcaatcctt tttacaattt gtccgtacgt tgtgggcaga tggacaagta
     7501 tttttaccgg aaaggatcta tcttctatca gaaaaagaac aacttctgta cggaaaaatt
     7561 gtagaaatat tggaagagtg gaaaaagaaa aatcaaatcc aactaagatg gaatgaaaat
     7621 ttcattcgaa aattcgtcaa aagtctttct ttgatcaaca gtgaacaagc ggggagacag
     7681 gaaatagaaa tttttgttgt ttcagaaagt tcagtcaaac aattttttta tcgaacccaa
     7741 tttcatttgt atgcggaaga aggcatcgct gaaataaatc ctattttatt tcgttcatta
     7801 gaagaattgc ctgatgaatg tctatgtgcg aaaaaaagag tggttttgtg cgatgctgct
     7861 agctatcaaa atggattaga aactgaaaag acaaagatct atccaatctc attaaaaggt
     7921 gcaaacatcc atttgtatca tattgctcag gaacttcacc tattaaaaaa acatatttct
     7981 tgatgagacc ttaattagga gaccttctac aaagcttttt aattgtctgt agaaggtttt
     8041 tatttgagtc ctgattcgtg cgaagatcta cttatataac aagctttctc tgaaattttt
     8101 ttaaaatgct attgacttag agtcaactct aagtagtaga atagataaag tgtaacaaat
     8161 ctattttcat taaaagaaga tagtacggag gagagaatgt attatgagta cagtattaca
     8221 tgtattggat gcaatcaata gccccgcaga tgtcaaaaaa ttatctgttg aggaaatgaa
     8281 acagttggca gaagagatgc gccaaaaaat cttgcaaaag gacagcgatg taggtgggca
     8341 cgttggtccg aatctaggtg tagtagaatt aacgattgcg tttcattatg tatttgattc
     8401 accaaaggat aaaatcgttt gggatgtttc ccatcagtct tatggtcata agcttttaac
     8461 tggtcgaaaa aatggttttg aaaaaggaca ttaccgtgaa gtgagcggat attccaacca
     8521 acatgaaagt aaacatgact attttacagt ggggcacaca tctacatcta tcagcttagc
     8581 aacaggttta gctaaagcga gagatctgaa gaaagaatca ggaaatgtgg tagcatttat
     8641 cggtgacggc tccctaagtg gtggcttagc ttttgaagga ttgaacaatg gtggtgtttt
     8701 acaatccaat ctaatcgtat tagtaaacga taatgagatg tctattgatg agaatcatgg
     8761 cagtttatat aaaaacttag ctgagcttcg tcaaaccaac ggtaaaagca gcaataacct
     8821 atttaaagca atgggatttg attatcgtta cgtggataat ggaaacgatc tggaaacggt
     8881 gatccgttta tttgaagaag taaaagatat tgatcatccg attgtacttc acgtccatac
     8941 ggaaaaaggt cgcggttatc agccggcaat cgataataaa atgaaatatc attggcatgt
     9001 accatttgat ctgtccaccg gggaggcaaa gcaagtaact tctggagaaa catttaatat
     9061 gatcatcatg gattatttgg ataagaaaat gcaaacaagt acagctccta ttgccgcagt
     9121 caatgcagcg ataccaggcg cctttggact gaaaacattc caagaaaaac atccagatca
     9181 ttattttgac gtggggatcg ctgaacagca ctctatcact ttcgcttctg ctttggcttc
     9241 tcagggagta cgtccagtcg tcttccaatc aagtactttc ttgcaacggg catatgatca
     9301 attatcacat gatttagcga tcaacgagaa tccagtgatt attttagtcg gtggcggaac
     9361 gatctcagct agtgaccgaa cacaccaagg aacatttgac attccttact tatcaagtat
     9421 cccgaacatc aaatatcttg ctccagcaac aaaagaggat atgatctcta tgctagattg
     9481 ggcgttcgaa caaacagcgc agcctgtagc aatccgaatt cctacacatg gtgtagaaca
     9541 tggtacagct atcgcaaatg attatagtat tccagcctat caaacagtaa ataatggagg
     9601 aaaagtagca attttagctt taggcggttt tcttgaatta ggaaaagaaa cagtaaaagc
     9661 ttttagaaaa aaaggaatcg aagcaacgtt gatcaatcca ggaacgatca cagaaaatga
     9721 cgtagaaaca ttagataaat taaaagaaac acacgaatta gtcatcacat tagaagatgg
     9781 tagtttgtca ggaggatttg gcgaaaatat cagtcgttat tatggtgatt cttcaatgaa
     9841 cgtattaaat tttggtgcag agaaagaatt cacggatagc gttcctgtag ggcagcttta
     9901 tgaacgttat catttaacac caaaacaaat cgttgaagat agtctgcgtg tcttgaatat
     9961 tcaataattc attatgctca ccatccagat aattgtttat actagtatct ggatggtttt
    10021 ttcattataa aaagcaaagg ggtggatgtt atgtatcagc aagtagaaga atatgtcaaa
    10081 aagttccaag aaaaatatcc tagttatcaa tcagaaaaat taggtttctt ttcatttggg
    10141 gatacagaaa aaatggcaga cgagttgagc gaactagttg taaaaaagat aaagactggt
    10201 acatcaagtg gttacgatct gtataaacaa gagaatgagc ctttaccaaa agttggtcaa
    10261 ttagacgtgg tcctgaatgg aaaaaatgaa ccagtttgta tcatcaaaaa tataaatgtg
    10321 accatcttgc cttttagtga agtcacgaaa gagcatgctt ggaaagaagg agaaggggat
    10381 cggacattag cttattggcg gaaagtccat gctgagtttt ttaaacatgc ttataaagaa
    10441 gcgttgaaca tagaatttac agaagagaag ctggtcgtat acgaagaatt tgaagtaatt
    10501 ttcttttagt aagaaaggtc tgctgaaaga aatagctcat tattgtctcc tgaatagaaa
    10561 agggttacat ttttatatga agctgtattt ttatataaca ttactggagg agatattgtg
    10621 gatacactgg aacaaatcaa aggcggagtc gtcgtttctt gtcaagcttt ggaaaacgag
    10681 ccgctacaca gttcttttat tatggggaga atggctttgg ctgcaaaaga aggcggagca
    10741 gttggtattc gagcaaatac gacagaagat atcaatgaaa tcaagcgaat cgttgattta
    10801 ccggtaatag ggattatcaa aagagattac gatgggtcag aagtgtttat tactgcgaca
    10861 gaaaaagaag tggaagaact tttggagacg caagcagaaa tcattgcact ggatgcaact
    10921 tctcgtaaac gtccgacaga aaaaacaact gctgaacttg tccaaatgat tcacgaacac
    10981 ggtcggttag ctatggcaga tatttcgact tttgaggaag cgcttcaagc gcaagaagat
    11041 ggttttgatc ttttgtctac gactttagca ggctatacag aatattcacg aaagacgcag
    11101 acacctgatt ttgatttatt gaatcaaatc atagaacaag tcgatatccc agtaattatg
    11161 gaaggacata ctgactctcc tgaacaagta gagaaagcat atgaattagg tgctttttca
    11221 gtcgtagttg gatctattat cacccgtccg caattgatca ctaaaagata tgtcgaagcc
    11281 gcaagtaaag caaataacta aaaaacacgc acggcacaga ttcttgacaa aaaagaaaaa
    11341 ttgatagata gtttaaagca gattctaaga agatgaaaaa agcattactt ttcattttgt
    11401 cttcaagatc tgctttttaa cgaattttat agaaagaagg cgaaaccatg tttgaacgga
    11461 gtccttcaga attgacagaa cgtgaaaatt acaagttgct gattggttca gtcatcccta
    11521 gaccagtagc agtagtaacg actaaatcag caaaagatat agtgaatatc gcacccttca
    11581 gttacttcaa tatcgtcagt tcgaatcgac ccatcttatc cttggcgatc cagagaaaac
    11641 aagatgcagt aaaagacaca gcaaagaata ttcttgagac aagagaagct gttatccata
    11701 tccttgatga ggacaacgta gaagaagtaa ataaaacagc agccaatctg cctaatgagg
    11761 aaagcgaact gtacctgact gacttgactt taactgattc agtaatcatt tcagtaccag
    11821 cattgacaga agcaaaagtt cgttttgaaa catccttata tgaacatgtc gagatacgga
    11881 ataaaaataa agtaacagct gatttatttt tgctagaagt acagaaatat catttaaatg
    11941 aaacagtcta tgatgataag acaggaagga tcgatccaag aaaattgaat gctgtaagcc
    12001 gtttggcagg aaatgattat gcagcaatag gagaaatttt cacgattgca cgtcccgaat
    12061 aaaaaggagc actagttatg ttaaaaataa gctataaacc atcgacagat tcaaaagaaa
    12121 tgaaaaaaga atatgaaaca gtgaatgatt ttctgcaagg gcagtactta gaagttccgc
    12181 cattgcaaga ccattttgtc gttactactg ttactttaga tggtaaggaa atagaaatgc
    12241 cagatcaaac gattagcgga ttgtttaatt actttaacaa ataaatattt attccgaaaa
    12301 gtgaaaaaac atataaaaaa acacataaaa ctaaaaaatt atcaaagaat cctggtgcaa
    12361 ctgaaagaat ttgcaacggg attctttcaa cttatttgtt atttttttct aaaaaatatt
    12421 aaagattgtt aaatttcact atttcatttc aaattccatt gcctaatcat taggaaaagt
    12481 ctatcatgaa ctcgtaaata gaatatagta ataaccttgt taggtgaggc tcctatacaa
    12541 acataggctg ttgcccagaa acgtcgaaag tcgccaatgg gtaaaacaga aactgttgga
    12601 ttaaggcttt ttccaatagc taagaatgtt ttctttacgt tgtatagtgc taaaactcgg
    12661 acgatgagat gatgctaggt cagaattctt gttcgaaaag gtttctgacc ttttctttat
    12721 atttttgtga ctgacttatt tgatgagaca aactcgctcc tcacctgaca agtggattgc
    12781 tgtgtgagga ggagatgcgg tgaaaaaaga tcgattggtg gattttgggt ccttatttga
    12841 ctttgtccat gatcggtctt ttagaagtat acagtgccag ttcttatcgt ttattgcaag
    12901 ccgatgaaaa tacaaaaagt ctattattgc gtcaactaat tttcatattt ttgagttgga
    12961 gcgtgatctt cttagctcgt tcagtcaaac tacactattt acttcaccct aaaatagcag
    13021 gatacggttt agccttatcg attttctttt tagtattagt aagaataggg atattcggtg
    13081 tcactgtcaa cggcgcacaa cgttggatct ctctgtttgg cattcaattc cagccttctg
    13141 aactggcaaa tctttttttg attttttatt taagctggtt ttttcgtgac ggaaatagta
    13201 gcccaaaaga tctaaaaaaa ccattcctga ttacagtagg tataactttt ctgattttat
    13261 ttcagccaaa gattgctgga gcattgatga tcctttcgat tgcgtgggtc atattttggg
    13321 cagcggcggt tccatttaaa aaagggatct atctaatcgt tactttttct gcattgctga
    13381 ttggagcagc aggcggggta ttatatttag gaaataaagg ttggcttcca cagatgttta
    13441 atcatgctta tgaaaggatc gcgactttaa gagattcttt tatcgatagt catggtgcgg
    13501 gctatcaaat gacccattcg ttttatgcac tatataatgg tggcatattt gggcggggat
    13561 taggaaatag tatcacaaaa aaagggtatc ttccagagac agaaacagat tttatttttt
    13621 cgattattac agaagagctt gggctaatcg gtgcattgtg tgtgcttttc ttattgttct
    13681 cattatgcat gagaatcttt tgtttaagca gtcgctgtaa aaatcagcaa gctggattgt
    13741 ttcttttagg attcggaact ttgctattcg ttcagacgat aatgaacgtg ggaagtatcg
    13801 cggggctgat gccaatgact ggtgtgccat tgccatttgt tagctacgga ggaacaagtt
    13861 atctgatcct ttctttagga atcggaatca cgttgaatat ttcttcaaag atacaggccg
    13921 aagagttggc gctctatcga cctgaaaaac aataaagaga aactgagaaa ctctcagttt
    13981 gaattatcat aaagtacaat cttttatttt tcaagcgaga ggttctttta gaaagaggaa
    14041 ttcgcaatga aagtatctca aaaaatcata ttatctattt tgttactgct tacattagta
    14101 gttggctatt tttcgattga gtttatccat ggattttcgt ctgcaaaaca gagttcaact
    14161 gtgaaaaagg tagagccgaa aagtatccct accacactaa atgtggcttt gattggttca
    14221 gatgctcgtt cgaaagacga aaacggtcgc tcggattcac ttctggttgc acaatacaac
    14281 cagaaaacac aacaagcaaa actgatctct atcatgagag attcatatgt cgatattcca
    14341 ggttacggaa tgaataagat aaatgcagct tactcttatg ggggagttga tttattaaat
    14401 caaacattaa aggaaaattt caaatttgaa gctccctatt atgcaagtat cacatttcaa
    14461 gattttatcg attgcgttaa tgaactgttt cctggtggag tcaagatcga tgcagaaaaa
    14521 tctctagatt tagatggcgt atatataaac aaaggagagc aagtgatgga tggtaatact
    14581 ttactccagt atgctcgatt tcgggaagac aaagaaggag acttcgggag gatcagaagg
    14641 cagcagcaag tgatcaaagc tatctctcag cagctgaaag acgtaacttc aatattcaag
    14701 ctacccaaag cggttgggaa attacttggt agtatccaaa cgaaccttcc agaaagtgtg
    14761 cttctggatt gtggtatgga tttcctcaaa gacatatgtt agaataaaca ggtataaata
    14821 gtgaaataaa ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac
    14881 ggcaaaaatc gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc agctgcgggt
    14941 ggcggttatt tttactacca gcactatcaa gaaacccaag cagtagaagc tggagaaaag
    15001 acagttgagc aatttgtcca agctttgaac aagggagatt ataacaaagc tgcagaaatg
    15061 acatcaaaaa aggcggcaaa taaaagtgca ttatctgaaa aagagatctt agataaatac
    15121 cagaatatat acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat
    15181 aaaaaagacg attctactta cagtttttca tataaagcaa agatgaatac ctcattaggt
    15241 gaattgaaag atctttctta taaaggaaca ttagacagaa atgatggtca aaccacgatc
    15301 aactggcaac ccaacttggt ttttccagaa atggaaggaa atgacaaagt aagcctgacc
    15361 acacaagaag cagcaagagg gaatattata gatcgaaatg gtgaaccatt agcaacaaca
    15421 ggcaaactaa aacaattagg agtcgttcca agtaaacttg gagatggagg cgaaaaaaca
    15481 gccaatatca aagcgattgc ttcctccttc gacttaacag aagatgctat caatcaggcg
    15541 atttcacaaa gctgggtaca acccgattac tttgtcccat tgaaaatcat tgatggagca
    15601 acgccagaac ttccagctgg agctaccatt caagaagtag acggcagata ttatcctttg
    15661 ggtgaagcag ctgctcagct gattggttac gtgggagata tcacagcaga agatattgat
    15721 aagaatccag aattaagcag taatggaaaa atcggtcgct caggtttgga gatggctttt
    15781 gataaggatc ttcgtgggac aacaggtggg aaattaagca ttacagatgc agacggtgta
    15841 gagaaaaaag ttctgatcga acatgaagtt caaaatggca aagatatcaa attgacaatc
    15901 gatgcgaagg cacaaaaaac agcttttgac agtctaggag gaaaagctgg atcaactgtt
    15961 gcgacaacgc caaaaaccgg tgatcttctt gcgcttgcta gctctccaag ctatgatcca
    16021 aacaaaatga caaacgggat ctcacaagaa gattacaaag cttatgaaga aaatcctgaa
    16081 catccattca tcagccgatt tgcgacaggt tatgctcctg gatctacgtt taaaatgatt
    16141 acagcagcaa tcggtctcga caacggcact atcgatccaa atgaagtgtt gacgatcaac
    16201 gggcttaaat ggcaaaaaga cagttcttgg ggatcttatc aagcaacgcg tgtcagtagt
    16261 gatgtatcac aagtagactt aaaaactgct ttgatctatt ccgataatat atatgcggca
    16321 caagaaacgt tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa attcattttt
    16381 ggtgaagacc ttgatttgcc aattagtatg aatccagcac aaatttctaa tgaagatagc
    16441 tttaactcag atatcttgct agctgatact ggatatggac agggcgaact tctaattact
    16501 cctatccagc aagcagcaat gtattctgtt tttaccaaca atggcacact tgtctatcct
    16561 aaattgattg cagataaaga gacaaaagat aagaagaatg tcatcagcga aacagcagta
    16621 caaacgatcg tgccagatct gagagaagtt gtgcaagatg taaatggtac agcacattct
    16681 ctttctgctt tagggattcc gttggcagcg aaaactggta cagcggaaat caaagaaaaa
    16741 caggatgtaa aggggcaaga gaacagtttc ttgtttgctt tcaatcttga taaccaaggt
    16801 tatatgatgg tcagcatgtt ggaaaataaa gaagatgatg attcagcaac caaacgagca
    16861 tcggaactat tgcaatacct caaccaaaat tatcaataaa aaattgcttt ttaagtacag
    16921 taaaaggact gtggcgttat cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt
    16981 ctctttcaca acttcttttt gatttactca cacttcataa actttttttg taaaatattt
    17041 gagaacaagt ggagaaataa gtgtacttaa taaaatcacg ataactaaag gtgaatagta
    17101 ctgtggttcg atcaattgac tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc
    17161 acgcgaaatc ataccagcac cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg
    17221 agcaccaaaa taacctccaa gcaatttggt caaaatcgct acaagcgtca atatagaaat
    17281 aaagagtagt tgttcactaa agtcagaaaa atcaacttct aatcctacac tgacaaaaaa
    17341 tactggtata aatactgcat atcctaaagc ctctacattg tggtatactt cttctttaac
    17401 ctttgtctga ctgacagcta tcccagcaaa aaaagcacca atgacagagc taagcccaac
    17461 aagatccgct acgtaagaca ttcctaaaca aataactagc gatacgatga taatagaaga
    17521 atttgcgtac actttttctg caaatgaaag taggaaaggc gcaatccatt tgactaataa
    17581 gaaaataaaa acaaaataaa ttagctgttc aatcaaaaca agcggtaaag aagcattgct
    17641 agaagcttcc cccgttagaa aagataggct gaaacttaga actaaaacga ctaaaatgtc
    17701 atctaccaca gaagcaccca aaattgttga tccttctttt gtattcacta cgtttagttc
    17761 ttttaatact tctactgaaa tactgacaga agttgccgca agaataatcc caaagaaaat
    17821 cgcttcattt cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa
    17881 gatgatccca agcaaagcaa cgaacatacc aggtcggaaa tattttttca aaagagataa
    17941 atcactttcc agaccagcta aaaacattaa taagatcaca ccaatctccg aaaagtcatg
    18001 gactagaata tcaggtttga cccaattcaa tccagcttgg cctagcaaaa caccaacaag
    18061 taattgtcca atcactgctg ggatacctaa tctccgcgag atatgtgagc caatcgttgt
    18121 tgaaatcaat atcaggcata aaataccaat aaattccatt ctcatcattc ctttgtataa
    18181 aatatagccc gaacttagca taacatacat tattgaaaaa ataggaaaat atactcacaa
    18241 aaaaaccaac aatccaaaag atttttttgg attgttggtt tttatacggt ctattttaat
    18301 aactaaaaat gattaacgta tagtcatttt tcttattctg atgaaattga taatctcctg
    18361 gatgcccatg aaaatcaaaa taaagccgaa tatttggaat agaagcatca aacttttgaa
    18421 tggattgaac aataagacga atccgccaat caacaacaga atactactat aaacactcca
    18481 acttttagct tttgtattca tgctgaagaa caactggaac aagctgttga tgataacagc
    18541 taatccaaga agaatcggca ggatagaaac gattgcaggt gcaaaagcat agacgatcag
    18601 agctaagatc aggaaaaaaa gaccactgaa cagaccgata ctccaagaac ctgttttcct
    18661 tttaaaacgg taagcctcaa aaagattcaa caacccaagc aaaacaagat aggcggtgat
    18721 cagataaccg ataaaatgaa aaacagcaga agggtttagc acaatcgcaa tcccagaaat
    18781 aatataaaca gcagctctta ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa
    18841 catataagaa aacctccttt gacttatttt aacgtgaact agtcatttta ccaaattaaa
    18901 tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa atcaacttct gaggatcttt
    18961 tccatttttt tgccttttgc tagctcatcg atcattttat ctaaataacg aatttctttc
    19021 atcaagttat cctcgatgtc ttctactcgt acaccacaaa caacacctgt aatcaaggaa
    19081 cgtgaaggat tggggacagg ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt
    19141 agttgatatt ctagttctgt ttgggaataa cccgtcagcc aacaaatgat ttcatctact
    19201 tcctctttta tacgatcttt tctctccgct tttgctacat acataggata aacgcttgca
    19261 aaacttgtcg taaaaatacg attttgcgcc ataatcagtc acctttcttt ttttcaaatt
    19321 atttactact tatagtatat aagaaaaaga gaagaaaaag cgattattca aaaaaacaaa
    19381 aaggattgtg acaaaattct tgtcaccatc cttttttccg ctctctgact ttttacaatc
    19441 ttcatagctc aagtgcgcat cagataatcg aatgctccta aagctgcagt agctcctgaa
    19501 cccattgaaa caatgatttg tttgtatgga ctatctgtac aatcaccagc tgcgaaaacg
    19561 ccggggatat tcgttgcgcc atttttctcg gtcactattt catttcttgg attcatttcg
    19621 atcgttccat ctagccaatc cgtattaggg agtaagccga ctagaatgaa tactcctgaa
    19681 acagctagtg tatgcatctc catcgtacgg cgatcgatat aggagagaga ttctacctga
    19741 ttcgtaccgc tgatttcttt cgtcgctgca tgtttgataa cagtgacatt cgcaagagag
    19801 actagtttat cctgtaagat ttgatctgct tttaattctg gtaagaattc tagaacatat
    19861 acatgttttg ctaacccagc caaatcgatt gctgcttcaa caccagaatt accaccaccg
    19921 ataacagcaa tctcttttcc tttaaataaa gggccgtcac agtgtgggca ataagcaatt
    19981 cctttatttt taaactcttt ttcgcctggt acattgatag aacgccagtg cgcacctgtc
    20041 gcgatgatgg cggtttttgc tttaagtgat ataccgttag ctaagccaat attaataaaa
    20101 tgttgctctt tatttaagga aacggcctgt tgattcatca taatgtcgat agggtaacta
    20161 cgcacatgac tttctacttg ctgcatcaat tttggacctt ctgtgtaaga ggtaccaatc
    20221 atattttcaa tccctagtgt ttcaacgact tgtccgccga aagaatcagc gacaagtcct
    20281 gtacggattc ctttacgtgc tgcatagata gctgcactac tagctgctgg tccaccgcca
    20341 atcactaata catcataagg ttctttttca aacaactctt cttcaatcaa agggccagtg
    20401 attttttcaa caatttgttc gatcgtcatg cgtccgctac taaattcttc tccatttaaa
    20461 aacacagttg gcactgccat gattttttta tcatcgattt ctttttggaa catcccacct
    20521 tcaatcatcg tatgagtaat atttggattt aatacagcta atatattcaa tgcctgtaca
    20581 acgtcggggc aattatgaca agtcaagcta acataactgt caaaatgcaa tgtttgtgtt
    20641 atttttttga ttttaagact caaagattca ctgattttcg gtgcccgtcc actaatttgt
    20701 aacatagcca gtaaaaaaga ggaaaattca tgaccgaggg ggataccagc aaaaacgatt
    20761 ccactttctt tgccaactgg attgatagaa aaactaggtg tgcgcatcga atctcttttt
    20821 tcccaagaga ttttaggaga catttctgct atttccttcg taaattcagc tagtttttga
    20881 ctattttcac tattatccaa actaacagaa aaaacaatag gagattcaat cagtgctaaa
    20941 tattgttcga gattttgttt agtttctttg tctaacatat ccaccaccta aatctttcca
    21001 acaagatcaa aacttggttt caatgtttcg ccactttctt tccattttgc cggacaaact
    21061 tcacctgggt gggtacgtac atattgagca gcgcgaatct tatcaatcag tgcactagcg
    21121 tcacgaccga ttccatctgc attgatttcc atagcttgaa cgattccgtc tggatcaata
    21181 ataaatgttc ctcgctgagc taatccttgc tcttcatcca aaacatcaaa aatacgagaa
    21241 atctgatgag acggatcacc gatcatcacg tattcgattt taccgatagc atctgatgta
    21301 tcatgccatg ctttgtgagt aaagtgggta tcggtagagc aagaaaagac ttcaacacct
    21361 aaagatttca atgtactgta ttgttcttgt aagtcttcta attcagtagg acaaacaaaa
    21421 gtaaaatcag ctggatagaa gcaaagaata ctccagttcc ccataagatc tttttcactg
    21481 acttctaaaa attctccttg atgataagca tgtgcattaa attcttcgat ttttttccca
    21541 atcaatgaca tagtaaaacc tccaataata attatttatt tataattatt gtaatttagt
    21601 tggagttgca aagtcaatgg atattttgtt atttaataaa gattactcaa aaacgaaggt
    21661 taattataaa aatagaagag gtcgcaagaa aatgctactg ctggaaatac tcgggataac
    21721 gttgagtaac agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc gtttgaaatc
    21781 gagcttcttc tggtttttta tcaactaaag ccttgagtaa atcataatgt tgttccatga
    21841 tcgtttccca tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt tctaaatggg
    21901 tattatggtc atctaaccag ttccaaactt cttttcttcc tgcaatttcg aaacaagctt
    21961 catgaaataa attatctgca cggaaaaaag cgcgttcgtc atttattttg actgcttttt
    22021 cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat ttggaacaac
    22081 attcaatcat gatttgctct tccaactgtt ctcggacaaa gcgagcattt tctgcagaaa
    22141 caagattaat tttggaaaca tacgtaccac tttgaggtat cgtatacacg agttcttgct
    22201 gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga tgttcctcca
    22261 gtgccttttc agatattttc cttccaggtt ctaagtcagc atagataatt tgtttacgaa
    22321 tcgcttgata agcttgtgat tgcaagctgc ttttcatctt tattcctcca aaatcatttc
    22381 tatgaacgta gtataacaga agaggtctga aaaacaatct ggtatgtcac attaactagt
    22441 aaatttgtat aaatagtaaa attttggttg acatagcgat ttttattgca taaaattcac
    22501 ttgaatacaa aaactaatat gttagtttag gaggggaaaa atgattgatt taaacaactg
    22561 gaggaacttt atccctgaat tatctcaaaa agaagtagaa atacctcatt ttgaccgtgc
    22621 atcattaaga gaaaatggac aaaaaaatcc tgtctggatc catttcggtg gcggaaatct
    22681 ttatcgagga tttcatgcag aaatcgctca acgtttagcg gatctaggcg aattgtccac
    22741 tggtgtcatt gtttgtgaaa cattcgatga agaaataatt gaaagggctt accaagattt
    22801 tgatgatttg attcttgaag tggtcatgcc aagcagtgga gagttcgtca aacgtatatt
    22861 agctgcgaca gctgacagtc tgtattgtca tccagattgt tctgaacact ttgataagat
    22921 gattcgacac tttgaaaatc ctagtttaca attagttact tttgctatta ctgaaaaagg
    22981 ttatcgattg aaagatacaa ataataaata tataggaccg attgcagtcg atataaaaaa
    23041 tggtccaaag aagccatcac atacaatgag tattcttgct gtgctacttt ataaacgttt
    23101 tttatctgga gcttggccga ttgcacttgt ctctacagat aatttttcac aaaacggtag
    23161 tcaattacgt acttctttgc taacgatcgc aaaagaatgg caaaaggaag gttatgtaca
    23221 aaatacattt atccaatact tatcgaatga agaaattgtt gcgtttccat ggacgatgat
    23281 tgatcgtatc accccgaatc cttccttgtc agttagcaaa atgttggcaa aagagggctt
    23341 ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat attgctccat ttgtcaatac
    23401 agaggacatc cattatttaa tcatggaaga tcattttccc aatggacgtc cgaaattcga
    23461 aaaagctggg gtattcttga ctgatcgtgc aacggtagat aaagcagata cgatgaaggt
    23521 gacgacttgc cttaatcctt tgcatacgtc tttagctatt atgggttgtt tactggggta
    23581 taggagcata gctgaggaga tgaaagatcc agatttatcg ctgctagttt ctgaaatcgg
    23641 gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc attgatccaa agatctttct
    23701 ggaagaagta attactgtgc gcttttctaa tccaaatatt cctaatatgc ctcagcgaat
    23761 cgccacggat acctctcaaa aggtggcgat tcgttatggt gagacgatta aaaaatatat
    23821 ggaaatagaa gttaaagacc cgcagacact tgtattcata ccattgactc tcgctgcttg
    23881 gcttcgatac cttattggca ttgatgatca aggaaaacca ttcatcccga gtccagaccc
    23941 actattgaca gaccttcagc ataagctttc cgatattcaa ctgggtacgc aagatacatc
    24001 acatattcat gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg gtgtagatct
    24061 ttaccaagca gagcttggca gaaaaataga agaaatgttc atcaagatgg tagcaggacc
    24121 aaatgccgta cgaaaaacat tacaggatta cttacaagaa catggaggaa atttatcatg
    24181 gaaatgactt ttagatggta cggatcacag gaagaaaaga ttaccttaga tgaaatcaga
    24241 cagattcctg cggtcaaagg gattgtaggc acattgatgg atataccagt aggagaggta
    24301 tggcctaggg agaggatcaa agaactaaaa gaagaaattg agtctcgtgg gttaacacta
    24361 aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc ttcccgtgag
    24421 cagtatatcg aaaactataa gcagacaatc cgaaacttgg cagaatatgg aatagaagtc
    24481 atctgctata attttatgcc tgtatttgac tgggtgaaat ctgaccttga ctaccgctta
    24541 gaagacggct catccacgtt agcttttata tctgatgata ttccagatga tccaagagaa
    24601 atcgtcgcgc gcatcgaaca ttccagcaat gaatttggat tgccgggatg ggaacctgag
    24661 cgcttagctc ctatcaaatc cttgcttgaa gcctatgcat cagtagacga agaaaaactt
    24721 cgagaaaatt ttgcctattt tctccaatca atcatcccaa catgtgaaga ggtaggggtc
    24781 aaaatggctg tacatccaga tgatccgcca tactcgattt ttggattagc tcgaatcgta
    24841 aagaatcgag aagatttaga ttggttgtgt cgcatagtag acagccctta taacggaatc
    24901 actctttgta cagggagtat tgcagaagat ccagaaaatg atgtatatgg gattttagca
    24961 gaattttgcc aaagagatcg aatcccattt gcacatgttc gaaatattaa atttttagct
    25021 aatcgagatt tctatgaggc accacataaa tccgaatacg gttcattaga catgtaccgc
    25081 atactaaaaa caatgtatga caacggattt aacggataca tccgtcctga tcatggaaga
    25141 atgatctgga atgagacagg aagaccagga tacggcttat atgatcgagc attaggtgct
    25201 gtttatctaa atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa agataattag
    25261 tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg ttcaaattct tgatacataa
    25321 atccggaggt tgttaataag cattcggatt tatgtatctt atttggtcta ggaagtaact
    25381 tgattttctt ttttcgattg atccatgagt gcttcttctt ttaattcaaa gatatattct
    25441 gagatcctca taatcacttt tttccagtct tctggtgtga tgattccttt taaaatatag
    25501 atttttttga tatgagaagg caattgtttc aaagggcagt cgctgataac gatgttgcag
    25561 ttctccataa aatcattttg tattttgatc gattcatttg aaaaaatttg ttctatctta
    25621 acgatcagat ctttactgat ataaaaatca cgcgtatagt tcacaccaat cacaattcgc
    25681 ggctgttttt tgtagcgatc atacaaatgt actaggtctt caaccatcca ttgcacattt
    25741 tccttttcta aaataactgg taacaagtgt tgattttgtt ctctgaaatc catataaaat
    25801 tgttcaatgg cggtttggac ttctttcaat acgctatgtt cttgataaag catgaaagta
    25861 ggaaggggcg tatttggttg attgaagtta agaaatccat tataaagttt atttcgtgcg
    25921 aatgccaatg agtaaagaat cttttctttt tcaggtataa acaaatcaaa tacagtacaa
    25981 agggactcca ccatttctct tgaataaaga acatgaggaa cgtttgcttt ttttaaaagt
    26041 ttgtattgct ccaagcttgt ttcttccgat tcgattttcg tgatagaaag tcgagctaaa
    26101 atattcacta agatgcgttg ttcgcgacaa attggcagat taggtcgcaa aatatctaca
    26161 gagagatcaa taaaaaagtc aatatattca tactctgtga attctttttg tacgtctttc
    26221 acagaagttg caggaaaatt ctctttacat atttttaata gaaaatataa tttatctatc
    26281 ataccgctat ttagtcggtc aagtacttct tttttaaaaa aagtattcat cataaaagcc
    26341 actacttgcg gatcttcatc ataaaattct gtagcagtac ttgaaaagat gttccaataa
    26401 atatctaatg ctgcatattg aatttgtacc tcttttccag aaaaagactt tctgccatta
    26461 ttagaaaagg agatagacaa accgtactat gctaaaaatg cattaatctt tttcattttc
    26521 gtatacaaat aagaggcact atagtttgtt tgctgactga aactgataat cgaatttgtc
    26581 tgacagctga acaaagaagt caagatctga taactgactg attcgttgca gtatcttcca
    26641 aatagacgat aatagcagta agccggatca attccttcaa aattcaactt taaaaaaggt
    26701 ttttgcttat caatatgcag ttctgttaga ttgagacgct caaagtcttg ttctaattct
    26761 ttgatgtata gtaaaaaatt tgattcagac atatggaaat gatctgctaa atattgtgta
    26821 gtaataaaga aacgatgttc agataggaaa agaaaaatat ctaattttgt attgcttttt
    26881 ttcttcaaga taaacgtttt aatcgcttct tgagtttgca tgaaagaggc tcctttttac
    26941 ttaaattgtt aaaacttgaa aatattactg tttttttcat aaatttagac aaaaaaacag
    27001 taaacatata ggtagaatct ctactatctt atttcttgca tacaaaaaaa tcaaggagtg
    27061 agagagttat gagatattca accatcttaa tatcaacatt tgcagcctta agcttatcgc
    27121 ttactataaa tcagacagtc cacacagagg agacagaaac agccaaatgg acagaaacca
    27181 agtgggccaa cgtcaatcga attacctttc aggatgaatt ttcatccttc aattcaggag
    27241 tcgcaaaatt aagaaaagga caaacagcga ccatcaaagc aaaaattgac tattcgggag
    27301 acttagcacc tatactacaa tcagctgtta catttgagaa tgttgatccc actttgtcga
    27361 ttggctatga cgagaacact ttgactatcc aacacaagca agcgatattt accttcaccg
    27421 ttacattaaa tcaggacttg ttaaaaccag cgctttttac tgtcaaggta tcagatggac
    27481 taccgaacga tcatcattta acgccgtata gtcagcggca gacaattgag caagattcag
    27541 ccatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa aatgaaaata
    27601 agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa atagaacctg
    27661 gagaagaacc cgatacagaa actcaaccag agccagacaa taaatcaaaa cctgaaataa
    27721 cccctggaga aaaaccagat gtaactccag aaccagatac ggattctgga aaccaaacag
    27781 taccggaaac aaatccagac acagataatg aaactgaaaa tccagaaaaa ccagaggtag
    27841 atccagaaga aaaaccagat gtgactccag aaccagatac ggacgcaaga gaccaaggaa
    27901 taccagaaaa aataaacaaa aaaacaattc aagaggatgg aaaaaaagag agtaaaaaat
    27961 caaatctagc aattctaaaa ataaatgaag agcagctgaa taaaaaatca cgaatatttg
    28021 atagcgccca atcagctgaa accttaaaaa gtagcaagga tacaaccttt gctgtcctga
    28081 aacgaaaaat aaacagctac caaaatccgg agagtcacaa aacaaagtta tactgtggag
    28141 cggaatcatt ctattaagta tagcaacaat gctttctgca aaaagattta aacaaaacag
    28201 atctctctaa catttgaata atgtctgtga acgcattttt caaataaaaa gaaatatcca
    28261 gaaaatgacc ccaaaatacc agattgaaat ctgatatttg gggtcgttca tactttatct
    28321 ttcttcatat ttattttaag ggatatctgt ttcttttgga agatggttcg ggatcttttt
    28381 cactttcaat acatctaaaa agaaggtaaa gatcggaatg ccgacaatca agccccaaac
    28441 gccaaagaac cgttcgctaa caagtaagac cacaaatgta taaaaaatcg gcagttctgt
    28501 tttgctggac atcaacttcg gattcagcac ataagactca attagatgga caatcaaaat
    28561 cgtaatcagg atataaacaa catctcggat gcctccttgg gaataagcta tgaaagatag
    28621 aggaatacaa gaaatgataa cacctgctac tgggatcaga cttaaaatga aaatcatgat
    28681 cgctaaactc agcagctgat gaaagccaaa tacagccaag gcgattgtag tgagcaacgt
    28741 atttaaaaga gcgacaacaa attgagcttc taataccaaa ccaaatgtat tgacaaactt
    28801 atcggcaaaa tagtaaatat cttgaaagaa ccaagaaaat tcacttttta aaaacaatct
    28861 tgagaataca attgtttttt tcttttcaat cataaaaaag aaacttaaga tgaatgacat
    28921 agcaaacgcc actgccattt ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc
    28981 attttgaagc tgttcaaaaa aatcgtttcg ctcaatataa gtatggatat attgtaaaac
    29041 aggatctgca tcttttggct gatgctcgta aaaattaata acggactgga tcatgtcaaa
    29101 tgtttgatga ataagcatcg ggatatactt tgtgaatgcc agatagataa ggtaaacgat
    29161 cagtgtataa atcatgatag ctaaaacacc tgtagggatt cggacaaatc gctgaatgat
    29221 ttgaacaaga tgaatcatca aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat
    29281 acttctaact aagaaaagaa caaaaacgac aaataataaa acagtgagac ggcgtatttt
    29341 ttcattcgcc aaaaaacgct gataaacggt caaatcgaca acctccaaat aacaaaaaat
    29401 aaacacgtta ttcattatat gatggaatat gaaaataaga aagagcaaaa agaaaaaccg
    29461 ctaaatgacg ttatcttatg agttggtttt cctcataaaa tcgttcatct agcagtttca
    29521 gccctttttc tggtcttgat gaaaattatc tagcattttt cgggtcagat gttgcagtgt
    29581 atccaattct tcctgttcaa gtggaagata agacttgatc ttttgtggaa taccttctgc
    29641 ttgtttttgc aacgcagcac cttgagcagc cagcgtaaca tttacgattc tttcatcacc
    29701 cgtttcacgc tgacgattaa tataattttt ttctgctaac ttttttagta agggagtcag
    29761 cgtaccagaa tccagacaca aatcatttcc tagctctttc actgaagtct ttttcttttc
    29821 ccataaaact aacatcacaa gatactgggt ataggtaaga ttcagcggct tgaggagggg
    29881 agtgtagtgt ctaatgattt ctttagaggc ggcatataaa ggaaaacata cttgattaac
    29941 taaatataga tcttccattt ttccacctcc gctgttttac tatagtgtat catagcggat
    30001 ttacacaaaa aagtctattc actaactgct ttcagagtaa cttctatatt tccagctgta
    30061 gcttttgaat acggacacac tttatgcgct gcctctaata attcttgcgt ttcttccaga
    30121 gaaagacctt cgatagatcc ttcaatctct acacctaaga caaagctcgt ttcactttct
    30181 gaataaaggg aaacttgtgc actgacagta gaagccccag agatattttt ttgctgtttg
    30241 atcaattcta atgcactgtt aaaacatgca ctgaaaccag ctgcaaacaa ttgttcaggg
    30301 ttggttgcat tttcgacgcg tttaccagga gctgcaatat tcaattcgaa tgtacgatcg
    30361 ggattatgca cttctccaga gcgtccgcct gtgttgatca ttttcgtaga atacattttt
    30421 ttcataaaat taccaccttt aattatattt tatacaattg aattttacac aatataattg
    30481 tggaaagcaa agaaatggat tggcataaga taaataagag aaagcacatg aaacagacgc
    30541 gctttctcga atagcaaaca tatacaaaaa atatcagaga caaaaaataa tctcatcata
    30601 aacattacat atatttctca aaaagttgtt ttctttcttc aaaaattact tcacgatgga
    30661 cctgatgaag catccataaa taaccaaaag gatctctgaa cattgagtta gtcacaccaa
    30721 agtccatcat ttctgtcatt tcttgaatct ctacacagcc atttcgtaat gctttattat
    30781 gtgtgtcctc gatatcagag acgatgatat tgaaccagat cgtatttgga tgttcagcag
    30841 taggagcagt caaaccaaga gacgtgtttt catccagcat atggaatctc gtaccataca
    30901 aggtaaaaat gacttcatta ttgcctctag ggtaatctgt tgcctcaatc agttgtattt
    30961 catcaaagat tgtttgatac agttctagtg ctttcaaact atcatttaat acaaaatcga
    31021 tttccgttcc tttatgcata aagatctcct ctccttttca gtcgaacagt tactagattc
    31081 ttttattata ccagctaaag aaaacgcatt caaaaaaata gcggatttac ttaccaactc
    31141 attcgattgg atgataagta aatccgcgtc ccattacttc tgctacgctg ctgacaatga
    31201 caaaggcact tggatcgatt tcttctatca atggtttcat tcgggaaaat tcatgatcag
    31261 tcacaacgat caataaaacc tcttttgact ttcgattata cccgcctttt gcctctagca
    31321 gggtaaggcc acgtccaatc tcagcactta agcgaaggcg gatgtcttcc aaataatgtt
    31381 cactcatgac cataacagat ttctttcgat tgatacctgt ttcgatataa gtcatgacaa
    31441 ttgaagtgat tgcaatagat aaaatagcat aaagaaactc ttctattcca aaaataaaca
    31501 gattaagaga aacaacgaat gcgtctgtaa aaaataagcc aatcgaagta ttcaagtgga
    31561 aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc gctagaagag ttgtttttat
    31621 ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa aatgacagac aacaaccgat
    31681 ctgaagtaat cattgtttct ggaactattg caatgattaa aggaaaaacc aagcttccga
    31741 aaagtacttt aaaaaagggt tttttaccta aaaatagaag agctagtacc aacataacga
    31801 tattcaacac taaaataact atagctcggt caaagcctaa agcagcttcc agtaatattc
    31861 ctagaccgct gacacctcca gcagcaatat gatgaggccc taaaaacata ttgatagcga
    31921 ctcctaatat gaagagggca aaaataatct taatgtatgt ccatataaat tgtctcgttt
    31981 ctttcattta ttcacttcct taattcatta ataatactct tatcttatta taacttactt
    32041 ttcaccttaa gttaacttta attaataaaa aaattaggct agtctaaatt attatgtttc
    32101 attcttctag aat
//
LOCUS       DAFEHS010000025        30326 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFEHS010000025
VERSION     DAFEHS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.025"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..220)
                     /locus_tag="LP327_001235"
     CDS             complement(<1..220)
                     /locus_tag="LP327_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL1725361.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
     gene            complement(217..510)
                     /locus_tag="LP327_001236"
     CDS             complement(217..510)
                     /locus_tag="LP327_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725362.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(547..684)
                     /locus_tag="LP327_001237"
     CDS             complement(547..684)
                     /locus_tag="LP327_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL1725363.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(686..1117)
                     /locus_tag="LP327_001238"
     CDS             complement(686..1117)
                     /locus_tag="LP327_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725364.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(1131..1625)
                     /locus_tag="LP327_001239"
     CDS             complement(1131..1625)
                     /locus_tag="LP327_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725365.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     ESVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(1625..2074)
                     /locus_tag="LP327_001240"
     CDS             complement(1625..2074)
                     /locus_tag="LP327_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725366.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(2078..2695)
                     /locus_tag="LP327_001241"
     CDS             complement(2078..2695)
                     /locus_tag="LP327_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725367.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(2695..3603)
                     /locus_tag="LP327_001242"
     CDS             complement(2695..3603)
                     /locus_tag="LP327_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL1725368.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(3616..6375)
                     /locus_tag="LP327_001243"
     CDS             complement(3616..6375)
                     /locus_tag="LP327_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL1725369.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(6372..7076)
                     /locus_tag="LP327_001244"
     CDS             complement(6372..7076)
                     /locus_tag="LP327_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL1725370.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(7088..9397)
                     /locus_tag="LP327_001245"
     CDS             complement(7088..9397)
                     /locus_tag="LP327_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL1725371.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGGVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(9592..10056)
                     /locus_tag="LP327_001246"
     CDS             complement(9592..10056)
                     /locus_tag="LP327_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725372.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(10056..10682)
                     /locus_tag="LP327_001247"
     CDS             complement(10056..10682)
                     /locus_tag="LP327_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL1725373.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWTSSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(10689..11054)
                     /locus_tag="LP327_001248"
     CDS             complement(10689..11054)
                     /locus_tag="LP327_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL1725374.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(11044..11382)
                     /locus_tag="LP327_001249"
     CDS             complement(11044..11382)
                     /locus_tag="LP327_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725375.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(11372..11698)
                     /locus_tag="LP327_001250"
     CDS             complement(11372..11698)
                     /locus_tag="LP327_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL1725376.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(11679..11957)
                     /locus_tag="LP327_001251"
     CDS             complement(11679..11957)
                     /locus_tag="LP327_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL1725377.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(11959..13323)
                     /locus_tag="LP327_001252"
     CDS             complement(11959..13323)
                     /locus_tag="LP327_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL1725378.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(13336..13905)
                     /locus_tag="LP327_001253"
     CDS             complement(13336..13905)
                     /locus_tag="LP327_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBL1725379.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(13877..15064)
                     /locus_tag="LP327_001254"
     CDS             complement(13877..15064)
                     /locus_tag="LP327_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL1725380.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(15128..16855)
                     /locus_tag="LP327_001255"
     CDS             complement(15128..16855)
                     /locus_tag="LP327_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL1725381.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(16852..17304)
                     /locus_tag="LP327_001256"
     CDS             complement(16852..17304)
                     /locus_tag="LP327_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBL1725382.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(17416..17796)
                     /locus_tag="LP327_001257"
     CDS             complement(17416..17796)
                     /locus_tag="LP327_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL1725383.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(17793..18179)
                     /locus_tag="LP327_001258"
     CDS             complement(17793..18179)
                     /locus_tag="LP327_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725384.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(18181..18459)
                     /locus_tag="LP327_001259"
     CDS             complement(18181..18459)
                     /locus_tag="LP327_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725385.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            18507..19235
                     /locus_tag="LP327_001260"
     CDS             18507..19235
                     /locus_tag="LP327_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBL1725386.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(19381..19857)
                     /locus_tag="LP327_001261"
     CDS             complement(19381..19857)
                     /locus_tag="LP327_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBL1725387.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(19817..20164)
                     /locus_tag="LP327_001262"
     CDS             complement(19817..20164)
                     /locus_tag="LP327_001262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725388.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(20151..20336)
                     /locus_tag="LP327_001263"
     CDS             complement(20151..20336)
                     /locus_tag="LP327_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725389.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(20337..20609)
                     /locus_tag="LP327_001264"
     CDS             complement(20337..20609)
                     /locus_tag="LP327_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBL1725390.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(20606..20809)
                     /locus_tag="LP327_001265"
     CDS             complement(20606..20809)
                     /locus_tag="LP327_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725391.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            20934..21146
                     /locus_tag="LP327_001266"
     CDS             20934..21146
                     /locus_tag="LP327_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725392.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(21179..21373)
                     /locus_tag="LP327_001267"
     CDS             complement(21179..21373)
                     /locus_tag="LP327_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725393.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(21381..21569)
                     /locus_tag="LP327_001268"
     CDS             complement(21381..21569)
                     /locus_tag="LP327_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725394.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(21566..21706)
                     /locus_tag="LP327_001269"
     CDS             complement(21566..21706)
                     /locus_tag="LP327_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725395.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(22196..22561)
                     /locus_tag="LP327_001270"
     CDS             complement(22196..22561)
                     /locus_tag="LP327_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725396.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(22601..22903)
                     /locus_tag="LP327_001271"
     CDS             complement(22601..22903)
                     /locus_tag="LP327_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL1725397.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(22903..23202)
                     /locus_tag="LP327_001272"
     CDS             complement(22903..23202)
                     /locus_tag="LP327_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725398.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(23195..23359)
                     /locus_tag="LP327_001273"
     CDS             complement(23195..23359)
                     /locus_tag="LP327_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725399.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(23365..23634)
                     /locus_tag="LP327_001274"
     CDS             complement(23365..23634)
                     /locus_tag="LP327_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725400.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(23646..24395)
                     /locus_tag="LP327_001275"
     CDS             complement(23646..24395)
                     /locus_tag="LP327_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1725401.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(24398..25084)
                     /locus_tag="LP327_001276"
     CDS             complement(24398..25084)
                     /locus_tag="LP327_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725402.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(25090..25761)
                     /locus_tag="LP327_001277"
     CDS             complement(25090..25761)
                     /locus_tag="LP327_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBL1725403.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(25754..26095)
                     /locus_tag="LP327_001278"
     CDS             complement(25754..26095)
                     /locus_tag="LP327_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725404.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(26273..26971)
                     /locus_tag="LP327_001279"
     CDS             complement(26273..26971)
                     /locus_tag="LP327_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBL1725405.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            27058..27528
                     /locus_tag="LP327_001280"
     CDS             27058..27528
                     /locus_tag="LP327_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725406.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(27571..27750)
                     /locus_tag="LP327_001281"
     CDS             complement(27571..27750)
                     /locus_tag="LP327_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725407.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(27737..28075)
                     /locus_tag="LP327_001282"
     CDS             complement(27737..28075)
                     /locus_tag="LP327_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBL1725408.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(28091..28294)
                     /locus_tag="LP327_001283"
     CDS             complement(28091..28294)
                     /locus_tag="LP327_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1725409.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(28309..29085)
                     /locus_tag="LP327_001284"
     CDS             complement(28309..29085)
                     /locus_tag="LP327_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL1725410.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            29384..29704
                     /locus_tag="LP327_001285"
     CDS             29384..29704
                     /locus_tag="LP327_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1725411.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            29722..30153
                     /locus_tag="LP327_001286"
     CDS             29722..30153
                     /locus_tag="LP327_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL1725412.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         8511 a         6332 c         4659 g        10824 t
ORIGIN      
        1 tgtttcctcc ttcttcgctt tcagccgaga acattttgta ggttcgattt gatacaccca
       61 acacactccc taaaaacgcg ccaaaaccag taatgatgac aacacagata tctgtgtact
      121 gccaattgag cgctttacca actaacccca cgaaagtagc tagtgcggga ataattacca
      181 gtgcgaacca ttttagtact tcgaacgttt tattattcat tttcttctct ccctaaacaa
      241 agttttgatt tgttgcgtgt gttccaccaa tttttctgta tgtgtatcta atctttcatc
      301 gtgtttcttt agttcttcat gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt
      361 agtcagcaaa tctaaattgt ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga
      421 tgcagcaatc ggtctaatta ctaataaaat caaagaaacg atagcggtta ttgatcctgc
      481 tatcattccc cattccccta aattaatcat gtgacaactc ctttacctta aataaaaacg
      541 catcaattaa gatgcgctct tatctttatt aatgatttta tctgcttctt cgtctgtaat
      601 gcataatgga acgaaaacca ttacttgttc gttagtgaaa cagccccagt catacattag
      661 tttgatatct tcatatgaat acatgttact tcccaccttg taattgtttt ttgatttctt
      721 cgatttcctt ggtattttgt acagaagcaa gcatcgtttt tgaattcaac tgcgctaaac
      781 tttctgactt ttctttcaat aagacatttt cttgcttgat tgctacattg tttagcattg
      841 ctttagaatt tagctgtttc aattcatcat tttctgcctt cagcgtttcg tataatgcct
      901 taatattagc caattcatct acgctatcaa cagcattccc ttttttctct tcttcagtag
      961 ctaactcaac ccactgtttt ttatcaaaat caaatcttgg tctccagtta ggaactggtg
     1021 gtttaatctc tgtacagttt tcagggatat tttcttgatt gttcaaaatg atttgctcga
     1081 atccgtaagg cttgattgat ttgtaaacta ctttcattag atttcctccc ttaaattgaa
     1141 taagtgataa caaatgaata agctgatcca taacttgaat ttcttctcca tttaatggct
     1201 ccgtctgcgc caatagatag ttgagcactg ttcaaagtag aacggtctat tgacccaacc
     1261 agttgttcaa aactaattgg tgtccgatag ccttctggaa cagttaaaat aaccgactca
     1321 ttcccgccac tactttttcc gtttaaagcc acaaaatata tagaaactgt ttttccttca
     1381 cgataaagct ttgctgtccc tgtattaccg ttagtgacaa ccagatttac cacttcatat
     1441 ctgttatcat cagtcgtcaa aacacgttta ttattgattg ttacagaacc tgaaaaattc
     1501 ttatgtcctg caatggcttg gtctccatct gtctgcacca atgcttcttt accatcaata
     1561 gcttctacat gcgtttttaa gtatttagca actccgtctt cttttaattg aacaatatca
     1621 gccattaaac cgtccccact ttctcaaatg taatattcgc taatccatca agtttcactt
     1681 tatcggttgc tgacattaaa cctgctgtcg tagttgtagc attacctgga tttttctgtg
     1741 ctccagctgc aattccatcc aacttagttt tatctgtgga cgacatcaac ccatttgctg
     1801 tagttgtagc tacagctgta gttgtggcat ttattccagg atcaccttta tctccctttg
     1861 gtaaaacaaa attaaatcta gctgcagatg atgttcctac attcgtaaca gaagcggttg
     1921 aaccactaga aacggttcct atggtaattg ttgctgcttg gccaggatcg cctttatctc
     1981 ccttcaccgt tgttggtttg ccttctatag cattccaatg agtttgtgga taaacctgta
     2041 cattgctttg ttttattttt acgatatctg tcattctcta tacctcccca attttttcaa
     2101 aagtaaaatt tggaattctt tcgtttgtgt aattttctgc ttgatttaca gcttcttgga
     2161 atttttgatc tacatatgac tgattaacac caccagtccc actaccacct gtagaactaa
     2221 ttgttccatc ttctgcaatt gatatatttg cacctgcttt taatattttt agagattcta
     2281 attttccttt taattcttca gagaaattga agtctgtttg cttagttgca gataaaacac
     2341 cttcttcagt aacttctaag agttccccaa cttttatacc tcctaattgt tcaggagtag
     2401 caatcggcaa aatatatgtt ccaccttctc catttacgat cttttgaaac atttcagcag
     2461 taagaatacc atcgcttgtc tcgcttgcat aaggtagttc agtaattgca ttctctaatc
     2521 ctaaatcagc tttcgttaaa atgactgcac catatttacc attaaccgaa agaacttttg
     2581 attgtcctga tatcattttt tctaagccta aaactgcaga gacatgtgta attggcataa
     2641 attgacgttt aacaccagat tcatcagttt ccatcattct ctttacttta accatcaaat
     2701 caccccaact ttctcaagag tgaaaacatt ctgttttgga tcatcaacag ttgcaataat
     2761 caaagctcct tcttcaattg gaaattctac tgtttcaatt ttttcgactt cgtgattatc
     2821 tgaaaagaga tcgtcttgaa gaatatcagt cacttcaatt tcaccgtatt caatcgtgaa
     2881 aagtgctgct ttcatttttt gatacaaata atttagatcg gataggaaac gttcagaaat
     2941 cgattcatgg cgtacacctt gaagatctac acgtgcatcc attaattcgg ctaacattac
     3001 tccacctgga tcgatagact tcaaaatatc cttgattgat tcaaaccatg acaagtaatc
     3061 tgattcttgt ccttctctcc aatcttggaa actattctcc tgttcctctc tccagcgatc
     3121 aaattcttct ttcctttcat tcatccagtc cgtaaaatcg cctttatttt cattaataaa
     3181 atctgtcata tcagcaatta aatcttcaat tgattgccaa taagaaccca tttctccttc
     3241 tgtttttgaa acagcattga tgacaaaata agagaagtct tgagttgttc caatcaagtc
     3301 ttctccttta aatataatga aattggctgt ttgtcgatgc aaacactgca tggaatattt
     3361 atcaaaaata tatttgattt tccctttttt agcatccaca atttttgttt ctaattggac
     3421 tggatatttt ccgccaacaa ctgattcaaa atatacctta cattctgata aatcatatgg
     3481 aagaccattt tcgacaattg tagcttccat aacttcagtg tttttattgc cttgtcgaac
     3541 ttgaatcatc cccacgtaat tatagggttc tgttgtactt aatatgacat tccactttgc
     3601 cattaaatca cctccctatt ttggtggtat gacaatggaa gaaattgcac ttgcactata
     3661 atactgtcga tctaactttc cacaaatcat acctaactct gtgttttgtt cataagtttg
     3721 catacgacca ttagctaagc ctcggataac gccagtatgt ccataagttc catctgcaaa
     3781 ccatgaaccc acttgtccac ctcttgccca attaataatt gcaccaacta ctaattgatc
     3841 atatctaggg ttttgaatca ctttccaacc aacagcagac caatcatagg caataccaat
     3901 atcagatgct gcagaagtat ttcctatcac atgagttaaa ccatattttg ttccagcacc
     3961 catgccacaa ccacccagat atcctgaata ttctgcagac aagccataac actgaccatt
     4021 gccaattcgt tgtccaatca aagactctaa atgttttagt cctgcttctc cagtagctcc
     4081 tcctggtttt aaatctttaa atttgttata ccagttaact gcataatctt gacgttcagg
     4141 atgtgtcgct gccggacgtt catagtttcg ttcaaaagca tatgctgctt gtcttggatc
     4201 agtacaggcc ttaaatccat caaccgtcgt aggttgtact acgcccatcc attgtccatt
     4261 tgtaaatgtc caaataagca atcgaacttg tgcatctaag ctcgttattg gttctttaat
     4321 acctgcagca ttaaataagt tttgaacata aacttttcca tcccatgttg ctggaccaac
     4381 aagaggatat gaagaaccat cccactgaac taatccgtat gctggtccac ctatttgaac
     4441 agtatcagga tcaaaagttc ctcctgtttc ttgttgaatg ttccccaata ttccacatgc
     4501 agattgtttc gtaaatccgt tattacacaa aatatcgtat attctccaag ctctcttttc
     4561 tgcatctgtt tttaattcat caggataccc accaggtgtt tctcctccgc cagatggacc
     4621 gccattttgt cctggtatca cttctttacc tctaacagta agttttccct gaatatctaa
     4681 atcaccgaaa taatatgctt ttccgtttcc taataacaca aatccttttt ctgcagttgg
     4741 agaaattaaa atgtattttc catcgccatt tgtacgtatt accaaagaat tttcctcaag
     4801 aggtgtaggt gttacagcat ttggaaatgg atttcctgct gaatctgttg ttccgatcgt
     4861 tccaatggat tcttttctat tccagaattc catacctttt ttggttagct ccatgatttt
     4921 tttgttttca ttccaaattt gaagcgtgcc ttttacaagt tttaacacat caccataagc
     4981 attaaaggaa gtttcgaata cttctgcatt tattgttcct actttgatga agtcagcaac
     5041 aatttctcct tttgatgtga tagcaatacc gaacgggcca tttactccgt tgtctgaata
     5101 acctaagcca tttaaattcc aacgccacac tcgctttgca ttcgccacat ttggagtatc
     5161 catgataaga atctctgatg gagctttttc tggacgaaaa acgacatgcc caccagaatt
     5221 accagtgatc catgccgtcg cattcaacac attttgaact aacgtttctg ttcgattgtc
     5281 gattttcttt ttcaattctt gtgcttgagt atttactgcc gaagtgtaca gtgataaatc
     5341 attacctaaa acaatatcct tatatttccc aagcgttggg aaataagtgt attcgaccat
     5401 acgctctttt atctcgatat ctagctcttt cgccctaaca tgagcgacat caccaaagtg
     5461 taaagacgct agctcttgat acatgtcacc gtattcgagt gtgtgttcca acgccaccat
     5521 actgactgta tgagtagctt ttggctcatg gatacgatca ttgtcaaata aggttttgcc
     5581 ccatttgatc aattcatcaa ctgttttaca atctccgttt tcacgtttgg caatacgtcg
     5641 attctcgttg gttactccat caatttctaa ataaccatat tcgataggtt ctttgtcttc
     5701 atcataatcg ttatcaggaa caccgccaac caaatacaaa ctattgacaa ttgattcttc
     5761 atcaatttct tcttcgattg cttctaaatt gatgccaaaa tcaatccgaa aaccattatc
     5821 tgatccaatc tgttttacca gtttcaaatc atagttatcc atatcaagtt ccgcgctagt
     5881 cacgccagtc aaattttgat taccgttatt tgaaccaatg attgcttcaa ctggcgcgac
     5941 ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa agatagttaa atttttgatc
     6001 aaatgccaaa ctgtttttaa gattggtcat gatctgagac ccattgccgt tatccgtaaa
     6061 cgacttgaca ataaagttct tattggccat aaaaccaata tgccgagctg ttacagaaac
     6121 ggaggtcaaa ttctttttga cattataaat ttcaaaatat tgccatgatc catctggtac
     6181 cttcgctttt ataaaatttc ctttttttaa ataagagcga tactgtccgc ttcttgaata
     6241 gttaccataa aaacgatatt ggccattcag cacacgattg atttctggca aatcttccca
     6301 atcaactaac gacgctccgt tcacgctcaa atcatctggc atttttttat aagcataaat
     6361 aaattcttgc gtcacagata cacactccta ttccaaaacc gcacttcttt aaattttccg
     6421 gatattttta cctgattcca ttcaggctgc aatactggcc aatcaccacg cgtaaataaa
     6481 ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa ccacgattgt tgcagctaac
     6541 gtattaagta ttgttaggct tttatcaccg acagcgatcg ttatatctcc tccgttcgat
     6601 tctatttcca gatagggatg tgcaatttcg tcaccatgat caaaaatatc cataacacta
     6661 gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga gtggctgaca acgaaaagta
     6721 acatcgaatg tataaaagaa gccccactcg ttttcgaaag ggacttcttt atccatgctg
     6781 cagatagcct ctagatactt gtctggatca ttatgtgtga ttaattggct tttaccagtg
     6841 agccatcgtt tgacttctct cagtttcgaa tgtggaatag tgatcccttc tatttccaaa
     6901 tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc cgcttcggcc ctgaatcgtg
     6961 taagtttcgt atcgcttgtt cggcataata tctggaagct ctgcctcaat gatgcaattc
     7021 atatcaatta ctgcattccg atttttccaa ataaaatttg gttcatctgg attcataaat
     7081 tgtcgactca aataggaaca cctcccaaat cacgaatagc ctgtttgttt gctttagcaa
     7141 atttacgatt catacgatct aactcagatg gattgtttgc atcgactttg ccaatgtgta
     7201 tatgctgttc aatgctacct ccagaaactc ttccactgat ccctttgcgt ttttcttcgt
     7261 ctgataatgg agtaactgtg gttttaccat tcttggcagt cagtaactca ggtccagctt
     7321 caccaacaat cgcttgtcca ttgattagat gaccgccttg tgctaaataa ggcagttttg
     7381 aaatagaaaa tgattttcca cctacaccag gaacccattt tggtatcttt atattattca
     7441 atccaccaat aaatccattg atcaatccaa tcatggcatt aataggagct ttccctacag
     7501 caacaatacc atcgaaaata cctccaaaaa tatcaactag accttgccat gcttttgacc
     7561 aattccctgt aaatactcca gtgataaaat ctagaaaacc actgaaaatt cgttttccag
     7621 cattataaaa attattgaaa tttgctataa caccatcgaa aaatccacca aaataaccac
     7681 ttaaaaaatt aaatatttca acagctacat ctgaaacacc tttgaagaaa gaattcactc
     7741 catcatgaaa ccatttaacg ttattatatg cccaaattag tccagtaact aatgcgccaa
     7801 tagctataac tgctaatgca aaccagccac cagacattcc aaataaacca gcaagtcctt
     7861 gccaaactcc tataaaactt ttaactcccc caacgatttt tgttacagat cccatcagcg
     7921 aaccaagaac gattagtact ggtccaactg ctgccgctat accagcaatt gttatgatcc
     7981 aattctttgt atcttgatct agagaccccc accatttaga gaattcctgt aataaggaag
     8041 ttgctttttc aaaaaaaggc aaaaggctta cttggactgc ttcacctacg tcagccattg
     8101 ctaattttgc attgttcata gcttgatcgg cttgatcaat tgggtcgaga gtagcatcga
     8161 atgtgtctcc tacagctccc ccactttctc cagcagtttt tgctaaatct tccaggttca
     8221 aagtacctct acgaattgca tcggccattc taggaccgcc tttagtcccg aatacttctg
     8281 ctgctgcatt gatggcttct gtttcagaac ctgcattttt taccttgtct tgtagttctt
     8341 ctagtccttg acttaaagat tttccgtctt tcgcataagc cactgttgct ttagataaac
     8401 tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa ttgtccaagt aaagtgaccc
     8461 cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc gccatcaatt gctttttgca
     8521 tcaagtcgtc tacagattga ccagtattct gcgatgtttt cgttgttaca tcaaggacag
     8581 aattcaaatc atcatattct agtccataag catcaatagc ttgtcgagca gatattgctg
     8641 attgcgaaac atctgtatca ttgatttcag cgtactgtag gagataattg gttgaatctt
     8701 ccagtttttt atccatgaat ccaaattgtg tatttacctc accaatagct tccccaaccg
     8761 tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa agactgtgaa agtctatcag
     8821 cttgatcacc tgttgctccg gtttttgtaa tgatggtatc aagtgcctcg tcaacttcac
     8881 caaatgctgc aagtcctgct gctcctgccg ctacaatagg cgcggttact ccaacagaca
     8941 ttttttcacc aacgcctttt actttctcac ctgtttcttc gattttctgg agtttttttg
     9001 ctgtatcaac agaaacgtca ccttgttctt taagtgcatc gttcgtctgt tctaatgctg
     9061 tacgaagttt attttctccg gtttcagaat tgagtaattg tttataaagt ttttctgatt
     9121 cagcagaata ttcaccggtt tctttgacag atttttcata ttcctctctc agcatttgag
     9181 atcgttgttc agccaatcct aattgttttt ctaatttttg tttagccgct cgcaattttt
     9241 ctgtttgagt tgcatccttg tccatcgcag acacttggtt tttgtactcg gcagcggcta
     9301 agttcatttc tttgttgatt tctttgattg ttttcgaata actgacttcg ccattcgttt
     9361 taaaattaag aacgacatca gattctttac cagccattta tcttagcgct cctttctacc
     9421 accaaggcga tttatccata gttacagatt gaggtggttc aaaatcggtg ttttgttgta
     9481 accactgtaa ataagatttg agccacaagt taggtgtaga ctttaaaaag aaactctcac
     9541 tccaattcaa tagagtgaga gcaacgtaaa tataaaaact ccaaggagtt cctatctctt
     9601 ccgattcttt ttgtttttct ttctttttgc ttgcgctttt tgaaagtctt gtggcttctt
     9661 ggattttttt aggtcttcca cctgaaatgt ctggctagca aaaatttcca tacaggtact
     9721 ataagcagac aacacctctc cactcattcc caaaaatttg aaaatagttt ctggatcttc
     9781 ctctaaacca ccagtacgca gcatggcata aatcaaggca cgcatgattt ttagatcact
     9841 aggcgataag ttagcagaag aaatttgtcc ttctttttta gatagcatgg cgttcatatc
     9901 agattcaaat tgcgaataat ctccaccata cacatctgct ataaattcca tcgtttgcat
     9961 agtaaatgaa atagggaatt ctgcgccttg aataattaca acagcagaat tttttaagtc
    10021 ttcaacgtga attccataat cagataaccg agccattaac ccgcacctcc aactttagcc
    10081 aatgttttcc actgttcttc atcgtataca ggttgagcaa tgaatttatt aaataattct
    10141 agagaagctc cgtctctatt tgaatcaaaa cttgcataca tcacattatt gtatttcagt
    10201 ccgatggaaa ccagatttgc agttacatcg tcaatttttg tttcttcctc tgcagtggca
    10261 tactcttcgt caatcacatt ggatagttgt gtattaggat accaaactgc ttttttaccg
    10321 ccattttcaa tatttccaat aaatccaaag gcgaagtacg gaaattctcg tgcggtattt
    10381 ttgctaaacg ttactccgct tttagcaact aatcctttga tttcatccat cacttctatc
    10441 ggaataccca cgtgatccaa cgcaagttca tgttttgttt ctcgcgacac acgacgaaac
    10501 attttgctgg atgtccattt ttctaatgct gttccattcc ctttgacacc gatttttgtg
    10561 gcaattggca atcgtaaaac ttcactataa gtcggtgcag ttccaacaga atctggcgtt
    10621 gccatcatcg caattaaaat gtcgtccaat ccttcaaaat aaaaagtatc ttgttttccc
    10681 aattaaaatc acccttccca taaatcaagt atttttctag tcataatttc ttcaattttt
    10741 tctttgtttt gttcatacgt accacttgca aaatgttggg cacgttgatt tgttgtgcca
    10801 ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt gaactttcac ttgatcgttt
    10861 tcaactacta cttttatttg atcgcgcata tgtttctttt ttaagagcga cttaggaata
    10921 ttaggaagca actgctttag ataaaagtta gctgcttctt ctaaagattc caacgataat
    10981 tttttcggat caacttttga aaggtttcct aaataatccg atatttcaga aaaaccattc
    11041 ttattacttg gcattttcca cacacctcac atatgtgtaa tagttggtta cggtatcgtc
    11101 gttctcatca ccttgtatac ctgtaaaatc tgaatatgga atgccagcat tttgcagcgc
    11161 ttgttcaata acaactaaat cctgttctgt tccaagtgta aaaaaagaga cttggtaata
    11221 aggtaatttt ttatagactt taccggatgc catttttttg ctgttactca catttgagta
    11281 cacaatgtat ggatacatcg tccctaatct ggctttgtct ctgaacactg ctaactttgt
    11341 tgatttcagc gctgttttca attcatcaaa gctaatcgac ataagctaaa ctcaactcca
    11401 tttctcttgc atcgggattc gtataaatgc gagtaatgtt atacgttaca gaatcaattt
    11461 tgagcgcact taatttctct gtgatggatt tatcccatct gactttaatt cgtctgacaa
    11521 cgtctgtctt ggcttgctgt gataaatatt tttcttgaga agtcacaccg agttcttcat
    11581 agaatattag acgcttaaat tcgtaaattg tagttggacg atcgtttccg tctgttcctg
    11641 ttttgatgtc tagcaattcg gctttccatc tgagattatt agtctgtctc ttcggcattt
    11701 tgaatcgctc cttgcacgat aaatggcgtc atggcattca tagctttgtc gagttcatcc
    11761 tctgaaactc tatattcata ggcaatgccg gcaaccatca aaataagata ttcttgttgg
    11821 ccaccagttg ctgttttgac ataatctttt gccatattta aataaaaaga gagcaaagaa
    11881 tcatccatgc cctcttcaaa atgaatatgt gatttgaatt tttcctctaa agacaattct
    11941 ttagtttgct cttccatctt aaccaccaac tggttttgta atttcgtagc gatatactgc
    12001 cggttcaaat ggagaataaa ccaattgacc atctagcaag ttgtaaattt ggaatccaat
    12061 ttgattttta ccagagaatt tttcaacaag tttttgaatt tccaaggcac caataacttc
    12121 ttgaatttta aatgcagaaa aatcgccaaa atataaaact ggcgtgtctg gttcaccctt
    12181 tttatctgct gcatctgtcc aatccacagg atagccaact aattggtaac caattccacc
    12241 ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca tctgttttca ttttttcaat
    12301 ggcagtcaaa gcagctcgat taataataaa gcgtcccttt ttcatcactt ctgtcactgg
    12361 tgtattttta aattcgatta atgcatcata taatttttgc ccagcacctg aagcagttag
    12421 atctaaaggt tttttaaatg ctacagcctt tttggctaat gcaccaggat tttcatttcc
    12481 agcgtcatca ccattgaaca tataattgat ttctttacgc acataagctt ttttcaattc
    12541 ttccacaaca atatcttcaa ctggaacacc agacattttt aataattttt tagttactgt
    12601 tgccaaagca tcgaattcgg caggattaag caaaatttca tcaaactgaa tagctgtttc
    12661 agcaatatca gttgaacgct ctttcttgtt tacattcgca tctgctttct tcacaagaat
    12721 tggatatttg acatctcctg atgttcgcac cactgttccg tatttacgaa gtaaattttc
    12781 ttcttgagca taagtaataa cttcagatgc aattacttct gggacagtaa ctgaaccgtt
    12841 gccagcttca atccctaaag ctcgagcttc tgcttcagaa atatttccag ctacaaaatt
    12901 agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg gtcatttttg cacgtgcctc
    12961 caatccgttc ttaattgatc caagtaatcc atctcgttgc tgttgagtaa tcattccaga
    13021 acgattttct gaactatctt cactgtctga atcttcttct tgatcgttgt cttcaccaga
    13081 acggctttcg tctgaatccg tactattcga ttgatcatct gtattgtcgt ctgtttcatc
    13141 agttccagaa tctgcgccta attcgtcttt aattccgttc aattcatcaa taacactgtc
    13201 aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat tctcctgatt caactttttc
    13261 acgtaattca gtcaatcgtt gctcacgacg tgccttcatt tttttcaata attctttatc
    13321 catgtatttt tcctcctacg cttctagcgc ttgattaatt tttttgatta atttttttct
    13381 ttcttcgacg tttttttcta attcatcacg acttcttaat gcagcttctg tatcttcgta
    13441 tgctggcaaa ggcacaatag aaacctcgta taattccact tcatggattg ttcgaagcat
    13501 tggttcagaa ttgtagtccc acgtttcttc agtcggcaca aaaccaaaac tacattggtt
    13561 gatgtctcct cgttccatag atttaactaa gtcccttgct accgttgtgt ccggtaaatc
    13621 aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga agggttccgc ttttagtacg
    13681 tcctagcaca ttggaccaat cgtgattgaa caaacaacga acatccgaat tactaattgc
    13741 ctttgagaat gcaccaggaa caatcacttc gcttaagtca tcccataaaa gtgtttgact
    13801 gttgaataca gcagcgtagc cgctaatggt cctcgtatga gtttcttcat cggaacgtgt
    13861 tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc ttcatttcca tcacctcctt
    13921 tcaagtcttg atcattcgtt ggtaaggaat catctgttgc atttttctgg ccaatcctag
    13981 ataagtcatt tgaaatatag atagcttgtg tttctggagt attctgttta ggaaaaccaa
    14041 gcatttctgc cacgttatct ggacttgtaa tcccggtacg aacgatgttg tagccaatat
    14101 ttgtttttgt tgagtaagga acaaaatcca aaatattaat tttccattcc actctatagc
    14161 cagaattagg cataaaaaaa agagcggtgt aatgttcgct cttgtttttc aatattggtt
    14221 tgattgcttt gttgtgcaga tacatcatcg ctttttcaat atctgtcttc atcagcgatt
    14281 gatacgtatc aacattgatt cctagaaatt ttcctaagtc ttttttgtaa acacctaaat
    14341 aattcaaaat tgccgcgtca tcaacaggac tttttaatgt ctcgatggaa tatccttttc
    14401 ccaaaggaat catcttaaca gaatgatcac tctcattttg cgttccttcc agttgatcca
    14461 atatagcttt gacaattttt gtttgggcgc tattatttgg attgatgtgg gcgtctagtt
    14521 taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc cgtcaaaact ttttcagcgc
    14581 tcagaacgcc ttctagagtg tttcttgcaa gatcaattat tccagcacct tttaatgaat
    14641 cagttccgat gttcttaatg tgtcgaatca tttgaccagg tattttttga ccattcattt
    14701 caaattcttc ttgaagtcgt tcattgattt tagttgttac accgtacgcc aaatgaagct
    14761 gatccctatc cgttaatggg aatgtctcac cattgatcaa taaagtattt gtttccaatt
    14821 tggtaaattc gaatcctgtc aaataatcat tgggactctt caaaatattt agtaagtgat
    14881 gatctttgac ttcgttgccg tcgggaccaa taacaattgg tgtggccaat gccacctgat
    14941 tagatatgtc ttgaactaat tcataaacat cagaagattc catgatagat gaatccgtca
    15001 catatctttg accataacgt gtgtagtgtc caaaaatatc ctcgatatat ccacgttttt
    15061 ccataaaaga atagactgca ttcgataacc gatcacgtaa tttcaatttt tttcaccgcc
    15121 tttctattta tctatagatg gaacttaggt aatcatctaa ctcatctgaa tcaatatctg
    15181 tcatttgatt catcgtttcc ttatgaccac acaaaaacgc cacgaaccca tcaatctttt
    15241 tctttgattg acgtttactt ggcgcttttt gtccattgat gttagtaatt gctacaacgt
    15301 tcaaagtgca ataaaggaat aatggattat caaattgaat tcgtttctca taaaacaacc
    15361 gttcgacatc gtcaaaagga gcattcaata ctttaggata ttgagcaact tcaacgcatt
    15421 ccaatcctaa gttttctaat ttctcaacaa gtttgtcact catcgctgga tcataattca
    15481 cttgctgaat atcatataaa ttcatgcagt cctctatgta ctgcaaaatc tgatcttgat
    15541 caatcatttt tccgtcgcaa aattcaacga aaccttgttc agccaaatca ctgtaaggaa
    15601 cattatcttc tttttctcga aactctaaat cttcattcgg aataaaataa agttgcttta
    15661 ctttaagaac cgctttacca tcttcatccc atgttgggaa gtttaaagat acacaggtca
    15721 aatctcgtgt acgtgataaa tctaaaccga tataacatgg ttcgccactt aaatcaccaa
    15781 gttcttgtgt ggtaactaaa catggctcta cttgatcctg ttcaaagaaa ttatccgcgc
    15841 cgttcacaaa tacatccaag tgtttcgtta gaaattcagc tttggaatga gctgaccgtt
    15901 gcgctgtttt aaatgccgat tctaaagcag ataaatcgac tgatattccc cagttaggat
    15961 tgcacatttc ccagactttt ctatccgtcc aatcataatt tttatttggc tcatagatta
    16021 gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc ttttgcttcg cgatatacac
    16081 gcattccaac agacgacgaa cctttcccag cagttgagat attaaacatc aatggctgtg
    16141 gtaaagatat ctgagcagac ttaaaattgt catactgttc cattttctcc tgtttatgca
    16201 attcatcgtt caatacaaaa tatggattgg atccctcgat attatcaata ttcttggttt
    16261 gaacaataaa tttatttgag taagccatat cttcatgaat atagtcataa gtaatactag
    16321 aaacagttcc ctttggcccc ctaaatattt tagttccttc aagtaggatt ggattgttta
    16381 agattgtaac tgcaaaaggt ttggcagcat attgcgcttg agcaaaatca gatgcgcaag
    16441 catagcaatc aactgataac gccccttctc cgtacattgc atatcctaat gaaccgacag
    16501 caatcaatgt tttaccattc ttttttggaa tctggacata agcctcacga gtaacgcgta
    16561 caatttggcc tttttcattt tctttaaccc aaccataaat ccaagaataa ataaattttt
    16621 cccagggttc taaaagaaac ggttttccca ccatttctcc ttttgtgtga acaataaatg
    16681 attctaccca atccatcatt tcattcgcgc gatctacatc aaaccaaata tcttttcttt
    16741 ttttccactg ataccagcga tctattgcta aacgaacggt cttaggatac ttcttaggtt
    16801 tctttcttac ttcttttgca aataaatcag cataatttac accaggttca atcatttttc
    16861 agcacctgcc ttcttacgcc atttatttct gtgcgctgct agttcatcta ctggctttac
    16921 ttctgggcgt gtaatttctt catcttttct tgcggtcgat cctccagcaa ttagtctacc
    16981 agtttttgct ttgtttgtta atcctaataa atctagagct ttcatcttct tatctgccca
    17041 agtttctact tgttgtgcca atggatgttt tgattgatta gtagctcctg atttattcgt
    17101 gaatttttgc gtctccggaa atcctttttc cttccacaat aaatatttgt gttggtaaat
    17161 ttcaaaaata tccaagtatg attcgattaa tggatcaaga gtgatggtgt acaaatcaga
    17221 caaattcatt atttctaaga tacgagcttt ttcagcactt actttttcat caataatcgc
    17281 tttacgttgc gctttagtcg tcatttttgt atacaccccc ctttgttttt tgaaaaattt
    17341 gacctaacga tgcgcgtgac tccccgctac cctatctccc cacgcgaaaa aattttgaaa
    17401 atgaataggg gggcttcaat tgaaatacga aggaaaaact tttttgtctt ccgtttcgtt
    17461 ttcaacaatt ggatgacatt ttgaacataa tagcatgaga ttgtttggat caagcttaag
    17521 cagttcgttg tctttgattg gtacaatgtg atggacgtgt gctctctttc caaatatgaa
    17581 ctgaccacac cgctgacaat gaccaccttc tctttcataa ataaattgac gcatatcttt
    17641 ccatgcttgc gttcgataga atggtttgtt ctcatgatga taaacagact ttgcttgctg
    17701 cttcttcttg cgtgatctgc ttgatctctt gtgttcagta cagtagatac cctttgctat
    17761 tttgttcgtg catccgtcaa actgacagta tttcattcta cttcacgaat aagatttatg
    17821 atatcgcctt ttgcacggat agcaccagga atatcaatac catgtttctt agcatatgca
    17881 cgcaattctt ttgcagtcat attgtccagt tcatccgtat cttcagtgga ctgatcatta
    17941 gtagcttcgt ctccatcaaa attagcagca gtgtttccat cgctatcaag aatttcactg
    18001 ctatgagcta tgagttcgcc attgacagca acaaatgatt tgccatcact caaaattcct
    18061 ttttcttctt ttacttcaaa atttggttct tgatctttcg gaacaactac tgtccttttc
    18121 ttttctgaat cccaatactc tgttccacta atggatgttc taattttgat cattgccatt
    18181 ttaattctct cctttgtaat ttgtacggat tacttttgcg cccattcgct cataccattc
    18241 aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa gaaataacta acgtattcat
    18301 tccagttctg taatcatcat tctccacttg cacatgtgca tgattaccat tccaattttc
    18361 tctgtacgga ctattctctg cttcaattgc tttcttataa actttagcca aacttgattc
    18421 tacttcaata ctaagtaccg cttcatgaaa atcattcatt tgtttactct cctatcagat
    18481 attttgtggt atgatttata aaaaagatgc gaggtgtttt aatgcttcaa gaggttaata
    18541 ttaagtatcg tgatcgtcaa aatacagaga caatttattt gccaaatagt tgtccgcatt
    18601 gcggaaatat aatgactccg tatgtgtatt ttggggtttc atcatatagt gcgttcgata
    18661 atgatagaat ctttggcgta ttagtacagt gtataaatga tgagtgcaac aaattttata
    18721 gtttacaatt tataaatacg tttaatgaag atgaaggata ttctgaaaat attttaatta
    18781 aagattatta ccgtccgccg attaaaatag atttaccaga aaatattgaa aaagtttctc
    18841 ctacatttgt tgatatttat actcaagcca cacaagctaa atctgagcaa ttggaacaaa
    18901 ttgctggagt tggttataga aaagctctag aatttctaat taaagattat gcaatatcta
    18961 aaaattcaga ggatgaagat aaaataaaaa aaatgttatt aggtaaaaca attgaaaatt
    19021 atttgaatga ttttcctaaa ctccaaagat tagccaaagc tgctacttgg attggtaatg
    19081 atgaaacgca ctatgttcgt agacatacta ataaagatgt tcatgatatg gatgcattta
    19141 ttaaatcagc agcgcaattt atcgcggctg attatgatgc tgatttagct gatcaattta
    19201 taaatggaaa taactttatg ccaactacta attaaaagaa taaaagagcc acagatacca
    19261 attaataaaa cggttgggat atcttggctc tttcatattt ttttgacact aatagaatat
    19321 catgctgcta acaatgttta catagctaca atgaccgaac atttagtgaa cagtgtaatt
    19381 ttagaataaa gagagttgtt ctttgtactt ctcgctattg taaaaatcaa tttcgttttc
    19441 ttttttcaat cggtcagctt gtctgtgttc atactcatct aaaaagttca atgtctttct
    19501 aatttctgca tgtcgctgtc tgacatacga tgagctatac ccaacagatt cagcaatatc
    19561 ttctaagctc atttgatcaa catatttcat cttaacaatt tgattatcga taccagaaaa
    19621 gctatctatc agcaataaca tttcttcttt ttgctcgatc aacaattcca attcattttc
    19681 tatcttttgg atattctctt ctaaagaaga agttctcgaa ttcttttcga tgcggacgtt
    19741 tgctaaatca ccacagaccc atcgattcaa ttcaagctta cttttattaa gattccactt
    19801 taagtaaaga atctgctcat ctagttcttg gtaatctttt aaccaatgaa atctcacaaa
    19861 cgccacccct tatggtagaa tagtattgcg ctgctatctc gaaaggggta gcttttttat
    19921 tttttcttag ccgttccatt ctggattgcc ttttgttcca aacgtcgttt tttcttctta
    19981 atctttgatt ttgtttttcc catacaattc tcctttccga aaacacattt tgttcgattt
    20041 ttaatactaa ataaaagttg gccagaattt ttcgttcgtc tttactcatc tttttttgaa
    20101 taagattact agcataaaaa tagttagtac cacgaaaatg aacaggtaaa tcaattacgt
    20161 tcaccttctt catgccagtt atcagccact agaatctttg cgttagtgcc gccaccgttt
    20221 tcttttttgt atttttcaaa tgctttataa gcagctgttt ttgatttata gatttttccg
    20281 gaaccgactg ggaacatcca tgtgccctct gtgatagcta agatgtatac tttcatttaa
    20341 ttcacccctt taatccccaa cactacatat ccggacttct gcatgtaatc agtaatataa
    20401 gtgatctctg ctttgtatga gtcaccagtg taactaaatg ttggtctcgt tagtccaggt
    20461 aattcatgtt taatgtattt tttatattca cgtagtatta actgatcacc aactttgaag
    20521 tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc tcgtcactgc ttcaaaatat
    20581 tctggtagta tttttagttc gtggatcatt ctgtttcctc cttcaaaatt cgctcatcat
    20641 caaaaagtga atctccacaa aatccttctc ccaaatattt atcgttcatg tataagtcaa
    20701 ccaaatattt gtgatctgct tctcgatatt cattaatatt aacgatcttt attttgtaaa
    20761 cattaccttc gtcgctcact atcttgaatt gttctctgac tttcaccacc gttcctcctc
    20821 tttttgaaaa aattttagtg tttttgtaag accccctaaa tcagcttttt tatttgatat
    20881 aatacttgta tcgattaatc aaatcgaaat taaatttaaa aaggtgattt atcatgaata
    20941 tcaacaattt agtaaaactc gttgaacaac gttttaaaga atcaggatta gaacaattaa
    21001 aaaaagataa ttctccagaa ttattttcta attttaccga agacgacatt cctactctta
    21061 aacaattaaa atattattct gattattgga cgcaaactcg tgagaatgaa ttaaatttag
    21121 aaaaactcat tattaaaaag aaatagcaac tccagcccac acggctggtt ttttagtttc
    21181 aacttccctc cttacgggtc aaaatcatcg gttccggcat aatctgatca tcctttagca
    21241 acaaatcagg atattttttc cttagcttct tattgttcgt gtaagttttc aatagccatt
    21301 ggttggcttc gcttttgctt tctccagaaa aataaatttt ccctgtttgg atgcctgcaa
    21361 gttgaatcat cattttcacc tcaaatcaaa tttttttctg ctagatggag caaaagcaat
    21421 ccgacatcaa gaatcttttg acccattatt tcagctactt tgctcggctt aactccttga
    21481 gaaaagagca agcgtgcttg ctctatctct tcgtgagtcc aaataaattt ggcttcctca
    21541 aggataatta ctggatcagt tgctctcatt gttcctctgg aaaaatgatg tcattcttat
    21601 gcttggacca ggtatctgca tatggaacaa agtattgtct agctaattcg atttgatcaa
    21661 ttaaagcagt ttctgataat tcatggtctg cagcaatttc attcatacta tcgccttcat
    21721 ctaaacagat aagaccgttg acgttatcga tttttacatt tgtgactgct tgactttctc
    21781 cacgctcata atattctaca gttgatactg atacatcagc ttcggagttt aattgcagaa
    21841 tctgttactg ccgggataat ttaatattct tttcataatt cacctttttt cctactgaca
    21901 tcgaatattt ttcatgttat gattagccgt gaaaggagag ctatattatg tgtttacaca
    21961 tgatgtcctt agctcattcg gttctttgga agcttttttg gaaataactc tcaggttctt
    22021 gacgcaaatc tcatttattc tttgaaacaa aaaacttcca ttttccgaga gctttggatc
    22081 acttcaaaag agatttgcat ttatatttct gtgaccttga gtcgcgtaaa gaaccttaca
    22141 gcaatggcgg caattccact ttcaggaaac cgcctttttt cattgatatt ggtggttagc
    22201 ggactaccca ttttctttcg gcagtgccaa accacaaatg atctgtgttg atgtttaggt
    22261 agttgtatac cagataagct aaatccaaag tgcctgcatc ttcaagacga caatattcca
    22321 aaaattctcc catagctgga cataaaaatc ctggagctat catatcccaa cagttaatag
    22381 tgcaaagaaa ttcgtttagc ttctgaacgt tttctttggt ttcacgaatt acaacatact
    22441 ccaattcttc tccgctggca gagcattcca aaacttcaaa ttgattaaga aatggtagaa
    22501 catatgcttc aaacatcaac attccatctt tctgtttttg cacttgtttt gcatcaatca
    22561 tgtctatcct cctattcagt tatttcttcc attaatggaa ttatttcagg tcactggact
    22621 taacgaaaac accattgacc atctttccag tccgaccttt gatttcgtca taagcaaaat
    22681 ccaagcactc gtataaatcc atatcatttt gcattgccaa gatgatcaag gttactacta
    22741 catcgccaat tccgtctctt aaaccatgtt catcttttct agctagagaa gcggcaactt
    22801 ccccgatttc ttcgaccgtt tttaacattt gcttgctgaa atccgcttga tccaatccct
    22861 tatcttttgc ccactgctct actttcgcga ttagttcgtc cattatttct cctccacata
    22921 cctaaactgt cttccttttg aatcaatcca taagctccta gctctatccc aaataatatt
    22981 tttgctcaga ccagtaattt cagataactg tttagcggta cccgttacta gaattcgatc
    23041 accatgccag attgcaatct ttctcggcgt tctccgtttg ggcttttcag tccacattga
    23101 tttaccgagc ttttggactt ctgcaactat ttttttatct tcctgccaat cttctgaata
    23161 agtcaattcg ataattcgct gcattgccgc tttcttatcc atcccgacat tctcctttca
    23221 gtaatttgag tacttgatca agtgcgctct cacgtccgcc atgaaatgtg ttgagccact
    23281 tgtcttcgta cgacacactt tgtcttaaag cttcttgatg cattaattcg atctgtgctg
    23341 taaatgtctt taggtccatc ttgtttacac ctgctcaagt tcattaagat gtttttgcaa
    23401 gcctttaaca caatcaatga atagtaattt ggtatatgct aaatttctta attgcgttcc
    23461 atcgatatag agtgcgaaat agtatctgag tttactccaa ctcgaacgat cattcttaat
    23521 tccttcaatt ccagcttctt cgagttgatc atatacgtct ctcaggacgt ctaattcctc
    23581 gcccttttta taattcgcta tttcattaat tagttctaga taatcgatct tcatttttcc
    23641 acctcttaga atggtgcttt tgattgtcta ttagctcgtt ctagcgcttt tttcttgaga
    23701 taggcttctt ggtcgattgc ccattcagga agcttctctc gtcttcctgt gcgcttgtat
    23761 ccactgcttg cgttcttagg ttcacttttt tctttccttg cccaacttcg aatagttgcc
    23821 aaatagtttt tataagtctt accagatgat tcacaatact ctgacagtcg ttcgattcgc
    23881 tcttggtaat cattagggaa ttctgttttg agtttctcca tctgctcatc tgacaaaaga
    23941 acatttttat actctccgta tttatgacgg acgggcttag ccttcgattt tttcgaaggc
    24001 ggtaactctc ttatatattc ttttgtatta ttaaatgtat tattaataga tgtattatta
    24061 tctttgactt tttcgtcaat aggggtattg cgtttttcgt caatagggta ttgattaatt
    24121 cgtaggtacc tattgattat ttgattggta ccctctttgt aaatgatttc ccgattcaag
    24181 tatccaaact taatcaaatc acttacccat cgcgatatgg tctctttatt cacaccatat
    24241 aaatctgcaa agtactcatt gcctgcccaa caaaagcctc tttcattaca caaggccgtt
    24301 atctctccgt ataacaactt agtatttggt ttaagtcttt tgtcgtacct tacgttggct
    24361 ggtataatcg cataataact tcgatgttct gtcattttta ccctccaata tttaactttt
    24421 tgattgtttt ctggtttaat ttgatccctt tgatttgata tttatttttg aaattaatca
    24481 cacctatttt gtgcttctct gtgtgatgga ttctgcagag tgctgcaaat gtgtactctg
    24541 catgatcaac ttctttgcgc tttcgtcttc ctagcgcttt gtcaaagtga tcgatgtcag
    24601 ctcctgtttt gccacagatg cagcagactc tttttgtgat gcatttgtag aagtaatatt
    24661 cttgattcgc aggtaaaatc tcatagcctt ctttgaaagg aatatgatgt tcaaagatga
    24721 aatctaagat gatatttgct aagacattgg catcactcac agttgtattc gattcgtctt
    24781 tcaggcttat tttgcgccct gtgacacctt caaaacgaaa gtagaagaat tccttccaga
    24841 agtccgttgg catgcctgta tcgataaaaa tatcgcctat cagcgcatag atgaagtttc
    24901 gttgctgtac ggtgaaacgt ctaggatcaa taaaacgaat ttcaatgact cgatcaccat
    24961 cgtagccgtc atacatcgtc tttagtcgat caatgttcac ttcctcattg atggttgcgc
    25021 ttatgtcttt tcctttgaac tttttcagaa ccgctgaata tgaatcgatt aatggtttaa
    25081 acactcatat cacttcttat ctaattcttt tctcttagct gctattgctc gctccatcaa
    25141 ggcacattgc tcatagctta actgttcaat agtttcaacg ttatcagcta agagccctaa
    25201 tttatctgtc tgctcattaa catattcgat taaggttttg gtcatatctt tacccatctg
    25261 ctcattgaaa gcttctagaa tcgtctctag catgtttaat ttctttgtat cgattctagg
    25321 tggtgttgga atatcttccc cttgaaatac atataatccc agtccgtgta gagccaatgc
    25381 tttcacaaag catcgcttca atgagttatt gatttgcatt gcatttggtt taacaactgg
    25441 ttggtttcga taatctaaaa caggaaataa ttcggtttcc gtgtgtcctt taaccgttac
    25501 tgagacagat acataagtcc cagtttcatc cataagaaaa ggtttatatt cctcaacaag
    25561 aaagtcttga tgagttccag aaacaaccct gtagtgtttg tactcattaa tagttaccgt
    25621 tgcctgtgga tcattctttt tcataatctc ccacgcgtga gcccaagata aataatcaaa
    25681 atttcctttt ttcttgagaa ttttatttaa cttacgacta aaaagttttt caaagttcgt
    25741 tgtccctttg atttcactca tcaaattctg cctccatttc agcaatgtat ttcttacctg
    25801 gtccgtaata agagatatca atcaagttat ctctgtcgta ctcttctagc gcatcaatca
    25861 agccatcttc gatgacgtaa atatattcag gtttattcga atgcttcgat agatggataa
    25921 gataaacatg atcccaaata gttacatggt tgcctaaatc atcttgatcc caagctagtt
    25981 cttcattcgt caaaagattt cgtctgattt ttcgaccact tgtttcctca attttcggct
    26041 tgccccaatc aggatcaatc aaatattgat ctagagtgga aagttctttt tccatatgct
    26101 aaaatctcct tatgatgtgt tttctttgtg actctttgct tgccggcgga gtcacttttt
    26161 tatttgttgc catgcttttt gcttgtcaat atgttgttgg cttaggatga ttggtttata
    26221 gtatttccac cagcaattag caattgccgt ccctattctt agcgcttcag ctctattcat
    26281 tgtcatcacc aaaaagtctc tgttgtctgt tcagttgatc aatttccata cggatcgcag
    26341 tttctggcaa ccacatttca ataaatgaaa cagcatcatc gaatctctta cgaggtaact
    26401 cgccatatct tgggattgaa aaggtacgtt taaattcaga ccaaaatttt gagaatactt
    26461 ttttgctgat ttcttcataa gctcggcttt cttttccccc tagaactccc ataactttca
    26521 tatttccttt ttgcttaatt tcaaactctt gttgtccgct aattcgcata gtatctttaa
    26581 gcatggaaac atctttttta acatctttca tttcttctaa ttgatagatc atcatatctt
    26641 caattgtttg aggaacagta ttcttgcgaa taacatcttc catttcgttg aatgcttcaa
    26701 tgtatttttg tttgaagtag atagctttct ttcctgtaaa ccccatagcc agcaagaaaa
    26761 atccatctct actaatgaag aaaactcgtc gatttctgcc atatgaatct ggttcattac
    26821 cttctacaaa catctgtcca aaattggaca catctttttt tagtgcatca atatctctta
    26881 aaacatgttg atgttttttc tcgaagcttt ctgccacttg taagctcgta gtcacagctt
    26941 ctttattttt caaaattact aattcttgca ttatttcttc tctccttttt ggtataattt
    27001 agttaaaaac tggtggtgtt tattttgaat ttttttcact taacaatttt tcaatggatg
    27061 tcaataataa gttttatatt gagtacgttt ttaacaggga taaaaataaa aggaatgcgc
    27121 cctcaactag aagttgaact taatgcttct tacttcgctg cagatatgat ttatacaaaa
    27181 gtaatcattt ctaactattc aacagaacca gctatgttag ttaatttaga actttcatcc
    27241 gctaacttaa accgcagatg gtcagctact ccatttaaga aattaattgc taaaggagga
    27301 tcagtcgatg ataatagaat atactctgaa gctgtaccgc taaatattcc tcctaaaagt
    27361 gctatatctt gttaccttgc atttgaagta gggaaaatta attttaataa actccttaat
    27421 caccaaacaa aaatgatatt tactctaaat agaacccaaa tccacaaaat tgttgatatc
    27481 aaaaatacaa acttcccaat agagaaatta gtgaaagaat taaactgagt aatagtaata
    27541 tacattctag tttctctttt ctattcattt ttagtcagcc ccctcggttg gctttttcgc
    27601 tctgtactca gcttcagctt catcaagccc cataaaaatc caaaccatgt aaacaatcgt
    27661 tcctatcaac gcttgcctgc ttccccaaag tcctaaagcg tagatgatta gcggtgcgct
    27721 gaacactagc gctctattaa attttcccat ccgcttgcct ccttaagatt tccgaaaaat
    27781 ttgtttctaa aaattcaagt gttttacttc ttaaaaatag atatgtgtct cttccttcaa
    27841 ctggataata gacaaatcca tttttgtttt tctcgatatc gataatattt ctatatcttg
    27901 ggttctttaa aactctagaa gtaaaccaat catattttct gttaatccgt tctagcactt
    27961 ctggcaacgt catccatcta ccagtatcat cagctttttt taactcctca taatccactt
    28021 gggagataat tacatagcct tctggaattg ggatttttgc ttctagatat tgcattagct
    28081 gttcctccct ttacaattcg tacatagtga taatcgaatc tataattctg tttgcttctg
    28141 cagaagtctt tttaccgttt aaaattaaag ataagtagct tttgctaatt ccaaatcttt
    28201 cagcaagcat ggtgtaagtt aagaactttg aactttcgac atattctttg attttttctc
    28261 tatctcgttg agtgatttct gcaatgtcag tcatactaaa actcctttct aaccaatttc
    28321 ctctaaatcc atttgagggt aatatccttc tttttttagt aattcgtaga taaatagacg
    28381 ccctttctgt gtccatttgg tattcattac aattttagtg ccaccatcgg ctttcgggat
    28441 ctcagttgta tgagattttg tgtatccttg gttcatgtgt tttttgcaca ataaccattg
    28501 gttaccgact tttttctgaa tacctagttt atgaagtaat ttattcatct gttgtggaga
    28561 catcccataa tctgctgcaa tctgactaat tgttactgaa tctgtagaag ataatatgct
    28621 atctaaatag gagattttag gttcgtactc tgcaatcttt tgttctgcga ttaatcttcc
    28681 agttctttct tcttttagtt gagttgctaa ttgaatgatt gtatctggat taagcaaagc
    28741 ttcttctact ttttctggag ttagataacc tccatgtttt ctaattgctg gcaacacttc
    28801 acttgttacc caacgtttaa attttttggc agagggaagt tttgatttta agattaaact
    28861 gtacaaacca gattcgttga tgattgtcat ctctcttgat tgacctgagg tcgtgattcg
    28921 cgaccccatc ttatcttcca aatctacatg ccgcgacaaa gcatctttag tgtttgaata
    28981 acccaaaaca ctcgcaacgt ctttgcctac aaaatatggt tcatcattta ctaaaattgt
    29041 ccgaacttcg ttttgttcga aattaaaaat ttgcggtgtg ttcatatttc tcattccttt
    29101 cttttatatt cgtaaacaaa tttaacaact tttttctaaa ttcaattgac agtatctgag
    29161 taatactcta tactaagagc ataattaaat aagacataaa aacattgatt ttaaagcttt
    29221 cttggcggtc tgcatttaca tatcaatagt gttttttgtt gtctttttag ttgttaaatc
    29281 tgtttacaag atatagtata gctcattact cagaattagt caaattattt ctgcgtattt
    29341 ttctaaactt tttttgtaaa catttagaaa ggttgtttta tcgatgaata cctatgaaat
    29401 aataaaagac ttggcgaaga ggaaaaagat atctatacgg cagttagaaa ttaattttgg
    29461 atattctaac ggttatctag caaagtggaa aaataatact cctaattcag atgaattaag
    29521 tcggattgct gactatttcg atgtctctgt agactacctt ctaggtagag aagaaagaga
    29581 aacccctaaa catgtggatt tatcagaaga cgatactgtt ttttcttttg atggaaaaga
    29641 aatatctaag gagacaatgc gtaaagcgat tgcaattgct aaagctttag aggaaaatga
    29701 atagttggag tgatggtttg tatgtatttg aaattgaaag aaatgttgag cgaatacaat
    29761 ttgaaagtag tttatatgga gatgaaagag cctggtttct actatccaaa acccagaatc
    29821 atttttttga atgaaaatct atacggtgaa actgcagaag cctttcattt atctcatgaa
    29881 cttgcacatt tcagcgcctc tcattttgaa ttttcagttt tgtacgatac ttctacaact
    29941 tttcactcaa aatttgaaac agaagcagat aaaatagcta ttctaatttt attaaatatc
    30001 tatattgaga acgaactgac tgatgaatct cagtttaatc tcgaaaaatt tatggagtat
    30061 tattctattc agaataagct cagatacact tgttatgctg tctgccaatg ctattttaag
    30121 aaaaaatatt cttacgcaag gcaatatgta tgaatacatc aagaattatt aaaaaaaccg
    30181 atttcagcaa aacattaaaa aagcccgtgc tgcaacacgg gctctttcct catatatgag
    30241 cttctacaaa aaaaatcggc tctttgtcaa taaggactga tgggttgtac aaaatcaaat
    30301 ctgggtcaga atgaacctca ttctgg
//
LOCUS       DAFEHS010000026        30041 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFEHS010000026
VERSION     DAFEHS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.026"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="LP327_001287"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="LP327_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBL1725413.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="LP327_001288"
     CDS             complement(738..1460)
                     /locus_tag="LP327_001288"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBL1725414.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="LP327_001289"
     CDS             complement(1596..2477)
                     /locus_tag="LP327_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBL1725415.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LP327_001290"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LP327_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBL1725416.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="LP327_001291"
     CDS             3717..4196
                     /locus_tag="LP327_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBL1725417.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="LP327_001292"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="LP327_001292"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBL1725418.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="LP327_001293"
     CDS             5497..5625
                     /locus_tag="LP327_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL1725419.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="LP327_001294"
     CDS             5629..6174
                     /locus_tag="LP327_001294"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL1725420.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="LP327_001295"
     CDS             complement(6628..8319)
                     /locus_tag="LP327_001295"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBL1725421.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LP327_001296"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LP327_001296"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBL1725422.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LP327_001297"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LP327_001297"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBL1725423.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LP327_001298"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LP327_001298"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBL1725424.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12601..13302
                     /locus_tag="LP327_001299"
     CDS             12601..13302
                     /locus_tag="LP327_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL1725425.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13911..14927)
                     /gene="tsaD"
                     /locus_tag="LP327_001300"
     CDS             complement(13911..14927)
                     /gene="tsaD"
                     /locus_tag="LP327_001300"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBL1725426.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14924..15388)
                     /gene="rimI"
                     /locus_tag="LP327_001301"
     CDS             complement(14924..15388)
                     /gene="rimI"
                     /locus_tag="LP327_001301"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL1725427.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15395..15937)
                     /gene="rimI"
                     /locus_tag="LP327_001302"
     CDS             complement(15395..15937)
                     /gene="rimI"
                     /locus_tag="LP327_001302"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL1725428.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15921..16643)
                     /gene="tsaB"
                     /locus_tag="LP327_001303"
     CDS             complement(15921..16643)
                     /gene="tsaB"
                     /locus_tag="LP327_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBL1725429.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(16834..17814)
                     /locus_tag="LP327_001304"
     CDS             complement(16834..17814)
                     /locus_tag="LP327_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1725430.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17838..19322)
                     /gene="galT"
                     /locus_tag="LP327_001305"
     CDS             complement(17838..19322)
                     /gene="galT"
                     /locus_tag="LP327_001305"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBL1725431.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19334..20323)
                     /gene="galE"
                     /locus_tag="LP327_001306"
     CDS             complement(19334..20323)
                     /gene="galE"
                     /locus_tag="LP327_001306"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBL1725432.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20571..20738
                     /locus_tag="LP327_001307"
     CDS             20571..20738
                     /locus_tag="LP327_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBL1725433.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(20800..22611)
                     /locus_tag="LP327_001308"
     CDS             complement(20800..22611)
                     /locus_tag="LP327_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBL1725434.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(22608..22973)
                     /locus_tag="LP327_001309"
     CDS             complement(22608..22973)
                     /locus_tag="LP327_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBL1725435.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(23136..23531)
                     /locus_tag="LP327_001310"
     CDS             complement(23136..23531)
                     /locus_tag="LP327_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL1725436.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(23549..24511)
                     /locus_tag="LP327_001311"
     CDS             complement(23549..24511)
                     /locus_tag="LP327_001311"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBL1725437.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(24511..24723)
                     /locus_tag="LP327_001312"
     CDS             complement(24511..24723)
                     /locus_tag="LP327_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBL1725438.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(24744..25442)
                     /locus_tag="LP327_001313"
     CDS             complement(24744..25442)
                     /locus_tag="LP327_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL1725439.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(25462..26004)
                     /locus_tag="LP327_001314"
     CDS             complement(25462..26004)
                     /locus_tag="LP327_001314"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBL1725440.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(26136..27140)
                     /locus_tag="LP327_001315"
     CDS             complement(26136..27140)
                     /locus_tag="LP327_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL1725441.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(27137..28126)
                     /locus_tag="LP327_001316"
     CDS             complement(27137..28126)
                     /locus_tag="LP327_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL1725442.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(28123..28929)
                     /locus_tag="LP327_001317"
     CDS             complement(28123..28929)
                     /locus_tag="LP327_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725443.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            29095..>30041
                     /locus_tag="LP327_001318"
     CDS             29095..>30041
                     /locus_tag="LP327_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL1725444.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATAANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKE"
BASE COUNT         8696 a         6369 c         5275 g         9701 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttaacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacatga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaac aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    12301 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
    12361 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
    12421 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
    12481 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
    12541 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
    12601 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
    12661 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
    12721 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
    12781 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
    12841 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
    12901 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
    12961 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
    13021 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
    13081 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
    13141 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
    13201 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
    13261 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
    13321 gtgaccctga taaatatcag ggtcactgct tattttcctc cctaaaactt ctgaaagatc
    13381 cggcagtttt agatcactgg acaagactca aagattttct atgtgtgttt cagttatgta
    13441 atattcactg tttgcaaaaa atactgttag attaactaga tgaagcgtct tgtcctcatc
    13501 cgatctagat tattcgatat tcctcgacaa taaacgatta ctcagtgagg gcaatcagag
    13561 gaacggataa tgataaataa gctgtaaaaa atagcaaaat aacaataatg attaaaagta
    13621 tgtaaaaaat agggctttga caaaaacgtg tcacagccct atttcgatta aacggtgtgt
    13681 acctgtcaac tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga
    13741 aattgttcct tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt
    13801 tcaaaagctt gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt
    13861 tcctgggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct ttattcagat
    13921 aaaaccaagc ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc
    13981 acatgtgctg ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc
    14041 actagttcta cctcagggag tttagctgcc aaagcatcct gcaatccttc acgcaaccct
    14101 tgattggccg ctacacctcc agctataatc agttgcttga ctggataatt ttgacaagct
    14161 cgaagtgttt tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca
    14221 tttttgtcta gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt
    14281 tttaatccgc taaaactgaa atcataatta tcttcatgga tcatcgctcg cggaaaatgg
    14341 taattatctt ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga
    14401 cctaatactc ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca
    14461 atgatttcat atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca
    14521 agaagtgcca tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct
    14581 gccatatggt ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca
    14641 gaaataccaa tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat
    14701 aaatcttctg ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt
    14761 tctacgtgat ggcggctagc tacttcaggt actacgccac caaaacgttt atgactgttt
    14821 atttgagaag caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc
    14881 gtttcatcac aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg
    14941 tttttaactt tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc
    15001 gtttacctaa tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata
    15061 gacgtacttc taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta
    15121 acaaatgccg tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct
    15181 cgacttcatc tattactttc gagtatccaa taaagccaca gatctggtta tcttcagcta
    15241 aaagcagata atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag
    15301 gtgaaccatg tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt
    15361 catcaaaatc tttttttctg caaatcaact aatctcactc attttctaaa agttttatca
    15421 tatccaacgc atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa
    15481 accccaattt ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca
    15541 tcgtctcaca gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga
    15601 ttcgtttacg ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga
    15661 acgctcttga accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat
    15721 gaggaagcgg tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat
    15781 tatagacttc tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta
    15841 gtacgtaacg acgatcgatt tgttcgactg ttttttctgt ataaatcatt ttatcttgaa
    15901 aaaagcttct ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc
    15961 gtctccaacc atttttcttc tgcttctact cgttttaaat agcgaggaag aaaagcttgt
    16021 ttctctgcaa ctggttcaac taaagcacct aattgagcca cagttacacc acttggaata
    16081 tcccatgctg gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcagagaag
    16141 aatttgtgac aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg
    16201 aacaaatcag aagcagaaat atgtacatct tcaagtacag tttctaatct attttctttc
    16261 cagcggtatg ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataata
    16321 ttatccacac cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct
    16381 ttatctaatg tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa
    16441 gaaccgggac catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct
    16501 gcaaacaatt ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt
    16561 tgtatttgtc caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca
    16621 gtatctattc ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc
    16681 attgtagcat acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa
    16741 tacatgatta tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg
    16801 tatcactcca ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa
    16861 ctctgtttcc acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat
    16921 cgtctccact gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg
    16981 cgttacatat ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac
    17041 cactaaacct ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc
    17101 aaaaagcgca tcaggaacat gcggattcgt ttttaaatat tgcttcactg cttgatatcc
    17161 tgactcgaca gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa
    17221 aacttgttga aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata
    17281 ttctgttcca gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac
    17341 tttttgaaca cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc
    17401 agaatctacc aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc
    17461 gaattttcct agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc
    17521 tttcaataaa gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa
    17581 ataataaaga tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg
    17641 cttagtattt gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa
    17701 aattttttgc tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga
    17761 aacagtagca ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct
    17821 ctcctccttt tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt
    17881 tcatctcgct tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat
    17941 ttttgtacga catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt
    18001 tctgcccacg gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct
    18061 acttctacta gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc
    18121 aacccaatgt tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca
    18181 ggatgttctt ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc
    18241 cttcttcgtg caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg
    18301 atagatactg tttcatccga atatgctttc catttttcca atactgagtt agctgcttca
    18361 ataagcaagt ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact
    18421 cctgcgatga cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc
    18481 ataggaaatt catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca
    18541 attggtaagt cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc
    18601 agtctagcaa atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc
    18661 gaatgttcat tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga
    18721 ttcattcgaa taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct
    18781 tcgttttcca tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct
    18841 gcgatttgtt ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca
    18901 tactccgact ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca
    18961 tttttcttac ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc
    19021 tgtgcaaaga atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct
    19081 tcaaaaatct cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg
    19141 acaagctgat ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct
    19201 agagcatctt ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc
    19261 atccatccgc cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac
    19321 atatctcatt cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg
    19381 ttttgataat cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt
    19441 tttcacttga agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg
    19501 gaatttcttt tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt
    19561 tgctgctgcc taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta
    19621 aaatatgtgc agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg
    19681 gtgtatcata atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta
    19741 aaatgattgg gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt
    19801 ttgctccggc tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac
    19861 accacttcat cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag
    19921 ctggtgtatc ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag
    19981 agaatacgat tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt
    20041 atacgttgtt gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg
    20101 cagcaaaatg gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt
    20161 tatcccggat gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg
    20221 tgagcaaatt atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt
    20281 gcgaaccgat ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga
    20341 ttagtaaaat tgtgtttgtt gattctatat aatttaaaaa acgaagttta cttttatggt
    20401 gaaaacaaac aaagtaaaat ttcgtaaatt catatggtaa atttataata aaacatttag
    20461 taaacatttt caaggttttt ttgcaaaggt ttacaaacaa acgtaaaact agcctttttt
    20521 tctcttttgt tccataatag atataacaaa taataaatga ggtggattca atgaaaaaat
    20581 ttgtatctgg aatattagta ggaagtctgg caacagccgc tgccgttgct ggtttggttg
    20641 cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg atcgaagaaa
    20701 accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata attatttaaa aatacgcatg
    20761 aagactttct aaaaaacaga taagtcatca tgcgtatttt tatttgtcag tcaatataag
    20821 ttgtgctatt ttatctgcct gtctcagatt ccatggatct gtataaggtc tagctactgc
    20881 tgcagttagc caatccacac cttcaacagg tattccatag caataaaagt cagactggat
    20941 ttcccctgaa gcatcaatca cgcgattcgt acctcgctca acatctatag cttctgaatg
    21001 ataacttcct gtattatctg aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt
    21061 ttttatgaca ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa
    21121 atagcgactg ctgatttctt tatcttgtag agaaataaca tagcggttgt gcttttgctg
    21181 aatatcaaat ggcggttcga tcaaatgaaa aatacccgca tctatcagtg ctgctaattc
    21241 tttcgtccga atgattggcg gaccgatcgt taaaaagcta tttagatgag taaaccatcc
    21301 ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc cattcaatag cctgccggat
    21361 tggatcacgc cagtctttta atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc
    21421 tttttccgct tcttttatct gcccttcaat aaatattcgg ctagcttctg aaaagggctc
    21481 attctgatag gcaagaggag tttctaaata cgaccaatcc catctataag ggattagtgc
    21541 tggatgcttt ccgagacact cctctgaaga gagggagaga aaatcatgct caaaattttt
    21601 ctttggaatc ggcaagtgat attcttcgat gattttcttg taataaaaca actcaacgtc
    21661 ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat gccattgctc
    21721 caggttttct tttgttagta accgtggcca tgccattgct ccgccttgct tttgatttct
    21781 tcctcgaggg taataaggga gcccccttct tgatccactg tatacaatcg gttccttgcc
    21841 tgatggatga tagaccagtt ttcccttttc gttctcgaag cgccctcctc ttgatactgt
    21901 aaacaatccg acataatcaa aaaaacttaa gcctagacct cttaaaaaga cattttcttt
    21961 aggcggtatc ttcgaaagat ctacatctgc aggattagaa ggaagctgat acagtaaaac
    22021 atttttcttt gcgtagtcta cccacactct ttcttcttct gtctcatcat tttcccagtg
    22081 accgcttgct aaaacaagct gatcgataga atagctttgc tgatctgtct gtaaaagata
    22141 accatctttt cttttgctta ctttttttac taaagaataa acaaactgaa tagaagctgg
    22201 aaattcttct tgcaactttg agaaaaacca gcgttgatag agtccgtata aacatcgagt
    22261 agattgctga tctttttcca gacgattgat ttcttctata attagttgct tatttttggt
    22321 taccgtctgt tcgatataat ctgtagcagg tcctttactc cattgaaaca agttgggtcc
    22381 ctgtgttacc aatcctccac tggacaatgt ttcatctgta aaaagcgtca cttgttgaga
    22441 aacagaattc attaataact ccggtggctg attcaaccgc caaactcgac ctccaggacc
    22501 attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc tgattattat ggagaatcct
    22561 ttctgctaca cttaatcctc taggtccggc accaatgatc cctattttca tttgcatgcc
    22621 tccatttcga taaatttttt caataaaaca gcatggttct gatgttcatt ttttgcacca
    22681 taaacaaaag taatgactgg atgttctttc ataagttgac tgagtttaaa aatagcttgc
    22741 ttcttttctt cttcgaaaat caattcttgc tggtatttct ttgcaaatga agcaaatcgt
    22801 tctggtacat ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc tttcaaccac
    22861 agatctaact gcgctttttc ttttgaaatt cctcgtggcc agaatctatc cacgagtatt
    22921 ctatagccat cctctttttc agcatcaagg taaacacgtt ttaatttgat catagttgtt
    22981 tccttctttc taccttttcg cttagcttat atatgtagat aatagcacaa aataaaaaaa
    23041 atgaacggca caactatcca ttgcgccgtt catgtgatct tggtcaaaat ctaatgattg
    23101 attcggtttt taaagctgtc atacaatcat ctgttttata cttttttact tttgattttt
    23161 ccagaccatc cgtcatatcc acccttaagt aaataaatct cttgataacc tgctttacgt
    23221 aatttatttg ctgtacggat gctcaaactt ttcttttgat cgtaaatata gacaggctgg
    23281 tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt aagggatgtt tcttgcacct
    23341 aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat cgattacttg tgcttttctc
    23401 attccttctc gaaattcttc ttctgtcaaa gtcgttgctg aacgtttggc cattactcgt
    23461 agatagactt cccaaaggat gattgcaagc acaatcaata gcaaaatgat attaattacc
    23521 cataaaacca tgtaatgcct ccgttttttt aagcaaattt caaggctaat gaggcagcac
    23581 caatgacacc agcttcatta ccaagctctg ctaatttgat ttttgttgat tctcttactt
    23641 gcgggaaagt aaattcttta aaatatttct ctacacggct acgtaaaaat tctcctgctg
    23701 ccgatactcc tccacctaac acgatacttg acggatttaa tgtattcccc aagtttccgc
    23761 aagctaaacc taagtagaag catacgcgat caacaaccat caatgcaaac ggatcgtctt
    23821 cttgagccat ttcaaagata tctttacttg ttacttcttc tccgttgtct agcattgctt
    23881 tcagttttga atcccctgcg tattcttctg ccaagaaacg acctaggcgt accacacctg
    23941 tagcgctaga tacagtttct aaacatccgc gtttaccgca agtacattcg aacccttcag
    24001 gatccacagt gatatgtccg atttctcctc cagctccgct cacgccgtga atcaaatttc
    24061 cgccagcaac gatacctccg ccaacacctg ttcctagagt aacgaataca acgtctggat
    24121 cgttttctcc agcacctttc catcgttcac ctaaagcagc aacattcgcg tcattatcga
    24181 tagcgaattt gattcctgtt cctgcctcga tcagtttttt cacaaattga acttctgtcc
    24241 agtttaagtt atacgcacca ataaccgttc cagcttcgct gtccacgctt cctggggtac
    24301 ccatgccgat tccgataaag tcttcggctt tcatattata taaattcaaa cgatgattga
    24361 ttgattcaat gatttcagga acgatatgct taccatcatc caaaatattg gtatcgatgc
    24421 tccatttttg ctgaatctct ccttctggtg ttaaaattgc aaattttgct gttgttccac
    24481 ctaaatcaat cccaataatc ttcttgtcca ttacaaattt cctttctttc ggctttcttc
    24541 aattcggtgc tctcttttta atatgcgcca cgctgtcatg tatgtatcat gatcaatcaa
    24601 acggctatca tacagattac gcaattcgat agtcattaat tcaatgtcat agatgcgtgc
    24661 acccacatag ataaagatgc caaatttttt gaacagctgc tgtacatcat ataagctctc
    24721 catctttaag cacttccttc atattataca agtaaaccat atttttttaa acccagtaaa
    24781 atacatatta caaaaagaaa tacaaaaact agtgtagaaa gaatcctttg gtgtatcgaa
    24841 tagcttgtct gttgtttcgg tagacccaaa acattcccta acactaatcc gccaattagc
    24901 ccaccgacat gtccccaaac atctacagag gtatccagaa gaccaaaaac aaatgtcaac
    24961 acaataaata aaagaaaatg tctgactagc tgctggatcg ctgggttatg tttaaaatga
    25021 aaccctaaga taaacaacgc accgaacaaa ccgaatatcg aagtacttgc accaccagac
    25081 agaacattcg cttcgttaaa agcaaagctt gctgtatttc ccattatccc gctgaacata
    25141 taaatcaaga aaaaacgcca atggccatag attgcctcga tttgctgccc cataaaatac
    25201 agtatcagtg agttcattac gaaatgcatc agtccaaaat ggatgaagat tggtgtcact
    25261 aaacgccacc attcattgaa gtgaacaaga taaggaccat acattccggc ataataaggg
    25321 atctcaagtc gcggaaataa ttccatcatg aggaagaccg ctgtctgtat ccctaaaaaa
    25381 gcataggtcc ataacggtcg tcttaaccat gcacgcaatt ttatttgctg ttgataattc
    25441 atgaaaacct cccatttcat tttagtcaat aaacaattgt tggatcttct gatcaaagct
    25501 ctccggttgc cattgttcat tcaattgttc acgaaaaaca aggctacatg tttttttatg
    25561 aaaattattt aaataccggt cgtaataacc accgccaaaa ccgattcgat aaccggctct
    25621 attgtagata atacctggta caatgaggag atcgatcatc tccggcaaat agagcttctc
    25681 tgattgtggc tcaagaacac caaaactcga acgtacaaga acggactgtt catccatttc
    25741 ataaaacgcc atctgacgtt tcggcaaagt tttaggtaca actattttct tttgttcttt
    25801 ttctgctcgt tggataaccg gctgtgtatc cagttcaaat tcattcgata aagtcgtccc
    25861 tatcactttt gctgacttcc agtactcgct ggcgaatagc tgtttataga ttcgctgttc
    25921 tttttccttt ttcttgccac ttttggccag atttttcagc ttttcgattc ctttccggcg
    25981 taatcttact ttcaattggt ccaaaaactc acccttcctt ctctcataaa atggtatagg
    26041 aaaaaaagaa aaatagcaat cgaactttca aataaatgca taaatagaat aaaaagaaga
    26101 cctagaaatg attggccttc cttttttaat gaatttcaac tgttcagcgc gtgtttttgt
    26161 ctgataaaaa gtgcatacag aaaatatggc gcaccaaaaa gggcaatcaa aattcctgta
    26221 ggtacttccc cacttggtag aatcactcta gcaaccatat ctgcactaag cagaaaaatg
    26281 gaaccgaaca aaccgctcag aacaaaggtc cagttatttt ctttacgtac gactagcttc
    26341 gcagcatgag gagctagtag tcctacaaaa gataaactac ctgtcaccgc tacactaccg
    26401 cttgccaaag caacagctaa caacaaaagg attttttgac ttttagccac gttaaggcct
    26461 aatccttgcg cgcgttcttg accaaatgat agcaatacaa tcctctgtgc ataaagtcct
    26521 cctagcggaa ttaagataac cgcccatgga agcaatgccc aaacataagg ccagctcgca
    26581 ccccagattg atcccgctaa ccatgcattc acaaatccat aattttcctt agataattgg
    26641 atcgtgcata gcaatgttaa tgcagaaagg ccggcattaa cggcaatacc ggacaataag
    26701 attcgattca tacccaaaaa gccatttcga tcataggccg caagatagac acagcaagca
    26761 gctaaaacac cacctagaaa ggctacaact agcaatagaa acggcagcga atagtgcgaa
    26821 aagaaaccta aataaagcat cactaaaaat cctgctccgg cattaatgcc caatatacta
    26881 gaatctgcca attcatttct tgttactcct tgcaagatat aaccagaaac gccaagacaa
    26941 gctcctgcta ataaactgat cagtaatcga ggaactcgaa attctctaac aatcagcgcc
    27001 aaagaagatg attctttccc tatgaacgtg gcaaaaagct gatccaggta aataaaaggc
    27061 gtaccaatca ttaaactacc tatagacagg atgacaagta caaaaagcaa gaaatagaac
    27121 gtccaatata tttttttcac cgttgttccc tccttacttg tatcagtaaa aacggaacgc
    27181 caatcaatgc aatgactaat ccaaaaggtg tttcaaaagg gggattgatc atcctcgctg
    27241 taaaatccgc caacataacc aatccaccgc caactaaaaa agagacaggt agaacataac
    27301 gatagtccgt tccaaccatt ccgcgaacaa ctggcggaac caacagccca ataaatgaaa
    27361 caggtccgac taacgaaacg gcaatggctg ctaacactgc aactagacag aaaacaatca
    27421 accgcgttcg tttaggatga cttccaagag ctaatgcatg tgtttcactc aaatgaatca
    27481 aagatattgc ttttcctaat gaaaaagaaa ccaaaacagc aagtatatat aaaggaaaca
    27541 atactgctaa ttgtttccat gtgatattgg cagctccgcc tacaaaccaa aaagcaagat
    27601 cctgctgcag attaaagaat tgggtaagtg cttggctgat agcagtaaaa aaagaactaa
    27661 tagctacgcc agctaaaatc agctgtaaag gattcatgcc cattttcgaa aactgcaaaa
    27721 aaccaaatac gagcaaagtt acagcaaaag aacctgccaa tgacattaga aacacagaaa
    27781 aatcagaagc tttaggccaa aaagcaaaac taagggccat tgccaacgaa gcacccgcgt
    27841 tgattcctag taagcctgaa tctgctaaag gatttcttgt cactccttgc atcaatgcac
    27901 cgctcaatgc aaaacaactc cctacaagaa atgctcccgc cacacgaggc aaacgaatat
    27961 ttcgtatgat ttggtgactt tgttttgatc tatcaaaatc aaacaacgcc tgccaaaaat
    28021 ctgctttttc cacagtcgct gcgccatttg aaagagcaac aaaaaaaata atcaaaagaa
    28081 caaaacagac caaaagcagg agtctatttt tcttcatagt cctcataatt gatcctcctg
    28141 ctcttttggc aacaaatcat aggttagaaa aactggttta tttgttcttg gaagcgtgac
    28201 aatcgttgca tcaatctcaa atattttttt catacactct tttgtgatca cttcttcagg
    28261 tgtaccttct gtgatcaact taccgttttt cattgctaat aaataatctg aaaaacgtga
    28321 agcatgatta atgtcatgaa tcgacatcaa gatcgttgtt tgtttcattt gattgatttc
    28381 agtcaataac tgtaggatct ctaattggtg tgccggatct aaaaatgttg tcggttcatc
    28441 caagatcaag atatctgttt cctgagcaag cgccatagcg atccagacac gctgggtttg
    28501 tccaccagac agcgttgcga tatcccgcat acgaaatcca gctagacctg tagcatccaa
    28561 cgcccactca attgcttcat gatcttgcgt gtccatgcca ctgaacagtt tttgataagg
    28621 gtatcgtccg taagatacta cttcttctac tgtcatacct gtagggtgtt cactagtttg
    28681 tgctaataat gcaatttttt ttgcaagttc ttttgaactg tatgtagaga tatcagagtt
    28741 attgacaatg atctttcctg atttcacagg tagtatacgg gtaaaacttt tcaataaagt
    28801 agattttcca cttccatttg gcccaatcaa actagtgatt ttcccttcag gaatcgtaac
    28861 agacatgttt tcaatgattt ttttgttttc gtagccggct gcaatgtcag ctgctcttaa
    28921 tctcgtcatt tcacccatcc tttcgctgaa aatcattctc aattatgacg ctgtttcaac
    28981 atttcgtcaa gcgctacttt catatctata gccaaatgaa gcggttgaca gcgttcgaaa
    29041 tatcaggaat aataaccaat gagaaagatt ctcaattatg ttaggaggaa agaaatgaaa
    29101 ttaaaaacaa aagtattcac attatctagc atattgctgc tggctggagt actgagcgct
    29161 tgctccccct cttcagataa agaaacagct acagctgcca attcgacgac ttcatccaat
    29221 agtactcata cagtcacaga tacacttgga cataaagtag agattccaaa cgcgcccaaa
    29281 cggatcatcg gcagttactt agaagactat ctgattgcac taggggaaaa acctatcgcc
    29341 cagtggactg ttggcagtgg ttccattcaa gattatctgc aagatgattt aaaggatgtc
    29401 ccaacgattt cttatgacct tccttatgag aaagtgttaa gctttgagcc tgatttgctg
    29461 ttgatttctt cttccgctac tgtcgaaggc gggaaatacg aacaatatag caaaatcgcg
    29521 ccaacttatg ttgtaaaaaa cggaacagat gtcacatggg aagaacaact aaaagatgtc
    29581 ggcaaagctt tggcaaaaga aaaagaggca gaaaaaatca tttccgatta tcaaaaatcc
    29641 atcaagaaaa ctcgagaaga actagcagat aaaatcgaga acaagacagc tgcagtgttg
    29701 tgggtgacca acaactcagc ctttatggtc agcaaaaatc ggtcaagtgg aagaatcgta
    29761 tacgacgatc tggaatttgg tgttcctgat ctagtggaag aggtctccaa aaaagccact
    29821 tctgactggt ctgctgtttc ctctgaaaag ctagctgaat tagatgctga ctacatcatc
    29881 cttgtgaaca gtgacaaaga tgctgccatg ttcaatgaac caagctggaa gaatctaaaa
    29941 gcagtgacag acaatcatgt actgcaattc ggacctgaat ctagttggct atataacgga
    30001 cctattgcca gtcaggaaat ggtcaatgat atcaaagaaa a
//
LOCUS       DAFEHS010000027        29612 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFEHS010000027
VERSION     DAFEHS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.027"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(403..624)
                     /locus_tag="LP327_001319"
     CDS             complement(403..624)
                     /locus_tag="LP327_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL1725445.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(802..2334)
                     /locus_tag="LP327_001320"
     CDS             complement(802..2334)
                     /locus_tag="LP327_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL1725446.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(2470..3555)
                     /locus_tag="LP327_001321"
     CDS             complement(2470..3555)
                     /locus_tag="LP327_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL1725447.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(3570..4289)
                     /locus_tag="LP327_001322"
     CDS             complement(3570..4289)
                     /locus_tag="LP327_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL1725448.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(4286..5596)
                     /locus_tag="LP327_001323"
     CDS             complement(4286..5596)
                     /locus_tag="LP327_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL1725449.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(5623..6213)
                     /locus_tag="LP327_001324"
     CDS             complement(5623..6213)
                     /locus_tag="LP327_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBL1725450.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(6531..7592)
                     /locus_tag="LP327_001325"
     CDS             complement(6531..7592)
                     /locus_tag="LP327_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL1725451.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(8160..9227)
                     /locus_tag="LP327_001326"
     CDS             complement(8160..9227)
                     /locus_tag="LP327_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL1725452.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(9247..9726)
                     /locus_tag="LP327_001327"
     CDS             complement(9247..9726)
                     /locus_tag="LP327_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBL1725453.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(9743..10204)
                     /locus_tag="LP327_001328"
     CDS             complement(9743..10204)
                     /locus_tag="LP327_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL1725454.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(10218..11468)
                     /locus_tag="LP327_001329"
     CDS             complement(10218..11468)
                     /locus_tag="LP327_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBL1725455.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(11846..13288)
                     /locus_tag="LP327_001330"
     CDS             complement(11846..13288)
                     /locus_tag="LP327_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL1725456.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKSTSCM
                     KEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIKRTI
                     DILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYMDAE
                     ERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQFFN
                     VLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVAKLD
                     TEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(13609..14373)
                     /locus_tag="LP327_001331"
     CDS             complement(13609..14373)
                     /locus_tag="LP327_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBL1725457.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(14401..15099)
                     /locus_tag="LP327_001332"
     CDS             complement(14401..15099)
                     /locus_tag="LP327_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBL1725458.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPSVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(15111..15890)
                     /locus_tag="LP327_001333"
     CDS             complement(15111..15890)
                     /locus_tag="LP327_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBL1725459.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(15906..16850)
                     /locus_tag="LP327_001334"
     CDS             complement(15906..16850)
                     /locus_tag="LP327_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL1725460.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(16896..17675)
                     /locus_tag="LP327_001335"
     CDS             complement(16896..17675)
                     /locus_tag="LP327_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725461.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(18055..20130)
                     /locus_tag="LP327_001336"
     CDS             complement(18055..20130)
                     /locus_tag="LP327_001336"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBL1725462.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(20132..21049)
                     /gene="glyQ"
                     /locus_tag="LP327_001337"
     CDS             complement(20132..21049)
                     /gene="glyQ"
                     /locus_tag="LP327_001337"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBL1725463.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(21435..22253)
                     /gene="recO"
                     /locus_tag="LP327_001338"
     CDS             complement(21435..22253)
                     /gene="recO"
                     /locus_tag="LP327_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBL1725464.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(22396..23295)
                     /gene="era"
                     /locus_tag="LP327_001339"
     CDS             complement(22396..23295)
                     /gene="era"
                     /locus_tag="LP327_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBL1725465.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(23310..23705)
                     /locus_tag="LP327_001340"
     CDS             complement(23310..23705)
                     /locus_tag="LP327_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBL1725466.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(23689..24165)
                     /gene="ybeY"
                     /locus_tag="LP327_001341"
     CDS             complement(23689..24165)
                     /gene="ybeY"
                     /locus_tag="LP327_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBL1725467.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(24194..26386)
                     /locus_tag="LP327_001342"
     CDS             complement(24194..26386)
                     /locus_tag="LP327_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBL1725468.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(26410..27381)
                     /locus_tag="LP327_001343"
     CDS             complement(26410..27381)
                     /locus_tag="LP327_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBL1725469.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            28086..29465
                     /gene="brnQ"
                     /locus_tag="LP327_001344"
     CDS             28086..29465
                     /gene="brnQ"
                     /locus_tag="LP327_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL1725470.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         8819 a         6088 c         4498 g        10207 t
ORIGIN      
        1 atagtaaaaa atcatctgcc tttttcatcc acctaatctt atttttcatt ttattcaaca
       61 tccttaaata aacttccgtt cctaagctct ataatatctt cttaaaaaat aatattatgg
      121 tgtcgttttc aaactcgtaa tcttttacat tggaaaaata catacttttc tcaatgtagc
      181 aactcctatc tcacagtatt caaaaattct tcaatattat gaaatacttg tgcatgttct
      241 ttaaatacct tgtcagtctg tttataatcc cctactaaat tttgcattgc tcccaactct
      301 tcgacaaatt gtcatctcca tctgtaaaca gccctaaaaa ctcatataat gctttttcat
      361 caatattttt cccatattat tctcggtata gtttcacaag ttttacggat tcttcatatt
      421 gtttatcttt ttataatatt catctaggga cataattact tttgctagat ttcctgcaac
      481 aacacaatcg tctgatacgt ccttgtttac taaactattt gcacctataa ctacattatt
      541 tcctatatgt acacccttta gtattctaga cttcattcct ataaaaacat tattacctat
      601 tttgatcctc tccgatgatc ccaaagcttc tccatactac ttttaaaacc gatcaatcat
      661 aaacatgagt caaaattgta actccttcat atcatctcca atttcaacaa gagtcttcgt
      721 tggaacaaaa actatacatc gttcgcctat tcggcagcct aaaaattgta gttactttat
      781 atatcattct gatgtctctt ctcatctttt taccaatacc gacgtaagaa ctttcaaaat
      841 atacttaaac ttcttattac ggatattaaa cagaagaata actaaaatcg atatgacaac
      901 cgatataact aaatttatca ctaaacttat cactttgaaa tttccgatat taacatacga
      961 acaagcaaaa taacttaaat aagaagcaac aaaagctatt acacacaaac ttatgagctt
     1021 ccaaacatat ttacctgccg attcaaattt aaatatattt tggaaaacat tatataataa
     1081 ccagggtaaa tcaaccaaca atactgaaac aatggttgcc gccaaaactc catataatcc
     1141 gaaccaatta acgaggatga tatttaatac aatgtttatc attgctgtaa ttaatggtct
     1201 aaacctgtct gcgtaccata ttccagcagc atctttgtat aacccaatta actgatcaat
     1261 ttcatataag aaaaaatgaa ctatcataaa tataacaaat gaaatactca taagattatc
     1321 acttcccacc cagataatca tgaacggctg aaataatgta ataaagcatg cacaactaat
     1381 tgccagaaac caacctataa ttgttgtaat atcacaaaaa tctatataat ttttttcttg
     1441 actttctaca ataagactgt tacctatacc tgctatacat gattcataaa ttattgcaaa
     1501 tatactcatt atggcagaaa taataaagta ataattctga taaatcgcaa gtactgaaag
     1561 acctaagaaa gatgaaataa taattgaatc tgaagatcgt aatattgtac cgcctacctt
     1621 gtttgtaact aatcctttta ctttttttat aatatcgtca atagtttctt tctctaactc
     1681 accaacagga gtataatcag gatacatctt ttgagaaaca acagcacaca ttaagttaag
     1741 aactatttgt cccaaaatag aagccaataa atacaaatag taatttttta agaaaatcag
     1801 aacatatgcc tgcattacaa attggaatgt aaaagtaatt agcatagaca cgttaattac
     1861 atcatttctt tgatgtgcat atattagact ctttctatat gaaaataacc aatacgacaa
     1921 aacagtattt atcaaataca ttaaatacaa aactgtcata tttaaatttc caggaatcga
     1981 acctttaact aaaaatctca aaaaaggaat gcataacaat cctaatgtaa acactatcca
     2041 tccaataatt gaaaagtact ttttatatgc gttcagtaaa gcacaaatct cttttctatt
     2101 gtcttctgct ataggtttat acatacttac agttagtgca gcacctattc ccaattccgc
     2161 taaatttaac atttgaaata tagatgtgaa caggttgttc aatccagcat actccattcc
     2221 catctgataa atcataagtg tacgcataaa aaagggaatg ataatctgat ataacttaaa
     2281 aattgttcca gtaattatat tccttttagc attttgttta cgattaatta tcatatcata
     2341 tggctccaaa ctttctattt tttattgtta cctaatacta tataatttct aaaatcttga
     2401 aatgtaatag gtttcttcta gctctttgat taattacgct aattttcatg tttggacaac
     2461 cacaaaaaac tatacatttt caatcaaagc aacccattgt tttgtaattt ctttcaaatt
     2521 aaatttttgt acattattat atgcgttcag tgacattctc attgctaaat cagtattctt
     2581 agtaaacatt atcatgctct tagctaattg gtctatattc ttatcttcaa ccaacatccc
     2641 attaaaatta tgatgtacca aatctctcgg accagtttta caatcataag aaataactgg
     2701 taatccataa gactgtgctt ctaatataac cataggaaat ccttcataaa cagaactaag
     2761 cacataacaa gatgcagatt tataatattc ctccacatgg tctgaatgcc ctgctatagt
     2821 tactctttgc agatttaaat cttttatctg ctggcgaaga tctttttcta attcaccttt
     2881 tcctacaatg ataagatccc aatcatctac ttttttttct acaattttcc aagcacttat
     2941 catgtcagca aaatttttct cttccactaa tcgccctact gcaagaaatc tctttttatc
     3001 ataatgatat tcgtactcga tattagatat acacggattt ggcatagtta taacaaaatc
     3061 ttctgataaa ctatattcat caatatgttt ttgacgatct tcttctgtga gaaccacaag
     3121 cctctttgca tattttttta tcaatttttt agccttgaat ctcttctttt ctttcagcat
     3181 ttccattgtc cagtaattaa aatgttccca agaaatcaat ttgcactttg ttcctttaat
     3241 tgcccttgta gtatacaaag acaacaaagt atcaatatca attactatat caaattcatt
     3301 tttgcatata tacttgtgca atttccaaac cggatataaa taagttctat ataattttcc
     3361 ctccgtttta ggatttaaca tataatcaac cttaatcgtc ttatctaacc caaaataagg
     3421 ctctccacta ttccaaaagc ttaaaatagt gacatcataa cctaaatcag acaagctatt
     3481 ggcaataata ctccctactc tttcagttcc cccagaacga gaaattacac cactaatgaa
     3541 acaaattttc ttcaaagtac ttttctcctt tataagtgta aaaatttttt cagtgcaagt
     3601 aacctgttaa attttttttg tccaattgtt gattttaatc tataaacaaa tttttgttta
     3661 ggtgttttcc aagatgcatt atattgatga atgcttattg tattactagt tttataaatt
     3721 tttccagtat cataatcctt ggcagcaaaa taatccgtag gatagatatg caggccattt
     3781 attatttgtt cgctgttatc gcgaaccaat ccaatagatt caagataatc tgtaatataa
     3841 tacggactcg gtttcaagtt caatgtacca tcattttgat aaaaacttaa tgaatcgtaa
     3901 atatccctta aacctttaat aacagaatgt ccttcttctg caccaaaacc aataccgcca
     3961 ttaacccaga tttccccttc aaatcccata taggcatgat tttcacgaag tatatctaac
     4021 gacttaatta gctcaacatc tgtatctaaa taaaatccac catgattata aataatatca
     4081 aatcttgcat aatctggaac aaaaccccat ttacgttctt tatatgcatc ggccatataa
     4141 cggttcttat ttaaatcata atttgattca ttccattcaa taatctcata atcagggcaa
     4201 tactttttcc agctttctat gcactttcta tcttttttcg gaagtggatt tcccccgaac
     4261 caacagtaat gtatcttctt aggaatcata atatctctcc ttttgtattt ttttcactat
     4321 aataatgctc tttccttttc acaacttcag gcatggctaa aatcatataa atcagtggcc
     4381 ccgttgtttc acccctaagt gttgtgttat acacaaaaaa gaataccgac ataattgcaa
     4441 taactttaac aagacttgca tctttatcct tcataaaaga tctttttgca ttgaaaaatg
     4501 tctttattat aaaaaatagg aatatcgaaa ctcctaatat tccaacttct ataaataacc
     4561 aaatatgatg aaaatactga taatgaagat taccaaacga tttataaaac tcactgacaa
     4621 aaaaattggt aaactctgca ttgccaatgc ctactcccat taggactaat ctaaaattat
     4681 tattaaatat ttcatctact actctagaga taccagttat tcgatttaaa tcaccaacac
     4741 catcatatcc agaatctctt gacgcatatt ctaaaagacc ttgaattgtg aaattatcag
     4801 catagctttg tccattatcg ttcaatgtaa taaatattgt tataaaaaag gataatacta
     4861 tcgacacaag tattacgcat gataatatct tgatcactac tttaaaatca acttttaaag
     4921 tgaatactat tgcaacaaat attataaata attcaggaat aaaaactttc aattcagcta
     4981 atgcagacat aaaaagtgct gcacaaacac ataaaataaa atggatgaaa tttttttctc
     5041 ccttgaaata tccgattgta acaacagtaa ctgtcacaca caaaattaaa ttcaatggca
     5101 tattacaacc tgaataattc caaaaaactc cacctatcat atctcctaca attgttccag
     5161 ttggataggt gaccatgaat ctttcaatta aacaaagtgg cagtgaaatc cagaataatt
     5221 tttcaattat agcgaatact ttatagtaat ctttaattgt taaatatgtt gcacaattta
     5281 tatatactat gaacatacga gtgttatttc gtatccccca gataatcaat cctatatttt
     5341 ctaaattaat aactgagcta caaatagaaa ctagtaaaaa caaaactatc agcatctcat
     5401 ttttgctagc atatattcct tttctgcgtc taaagctaag tgcatttaat aacacaaaga
     5461 tattaattat atctcccaaa tataaaattg ctcttggggc gttaaaagta tatacaagaa
     5521 ctatggagat tgcaaaaaat gccaacgtga acaatgtcca atctgctcct gaccatcttt
     5581 ttattgaaat acgcatcgtt attcaccttc tttcgctaaa aattattcta aaaatacttt
     5641 tgttaaatta acatacccta ttatttttgc cggtacacct gcaagaattg caccattctc
     5701 tagacaatct ttgataacaa tagctcctgc cgcaacaact acattgttag ctatttttac
     5761 attaccaata atctttgcac ccacgcatac acgtacatta tttccaatta caggacactt
     5821 aggatcgatt ccatcatttc caatacagtt attcccatat aagtaacaat tttttcctat
     5881 tatggcgtta ccattaataa caattccttg tgtatggtaa aatcttacac cttcatcaaa
     5941 tacattttca ccacactcta taccacattt tcttcccaaa ctattcattc ttctgcaaga
     6001 cacaatatac ataatcgcat aaatgatatt tttctttctc tttgaataat aataagatgt
     6061 aattctcatt cgacgagtat accgccaagc gttgtagtcc ggatgtgatt tcattatttt
     6121 tagtaatttt gtgtgttgtc ctttagattc tatatataga ttcttttcca tacgaagaac
     6181 cgcttttaaa tattctttac tgctaatttc cattcatatc tcctattaat tcaatttata
     6241 cttaacaatt ttattcggat ttcttgcaac aagagcatca ttcaatgcta ctgaatctac
     6301 aacgatggat atgttatctt catcgttgat tccaccggat acaatattac ttataaaaat
     6361 cgtaacattt tttccaaaag aagggatgtc cgtgatgtat aatgcccatt ttccgatatt
     6421 tccagttatt taaacctaac gaatttttac aatagtacaa gtatcacaat cattaatata
     6481 taactggaat ataaatctcc aaaaattcac tatagtacag ttttttatga ttaaaatctc
     6541 ctatttctac ccattccgct taaatatgct aatgttttaa ccccaataat ggattggttt
     6601 agtaaaaaat agttaaatat tcgttgttta attggatttt gtttatatgg gtatgagtga
     6661 atacaggcaa tcaactcatc gttcataagt atcttagaca aataggagtt taaatcaaat
     6721 tctttattaa aactagcata tctaactgcc ttttgaatac aagtacgaca gcgtcccaac
     6781 aaaagtctcc ttgttttgtt tacaaatatc tcattttcta atatatagct taaatcgcct
     6841 tcaatttttt taactaattc aatattacaa tcgaactcat tatctctaaa attatgtgtc
     6901 aatgaattat tattattaca tctataataa tagatacaat ctttaataaa acttgttatt
     6961 tttgaatgtt gcaaatatga aatatggaat atgatatctt cagatatata atccctttct
     7021 gacggaaact tgatattgtg ctgttgaatt atctcagatg aatacagcgc atgccaaact
     7081 gacattggca atataaaatc gactttataa ctcggctcag tgccaatcat tccaagcaac
     7141 actttttcaa ttatagcgtt atttgtaaat atcgtactat catatcttgc tggctttgaa
     7201 ataatattat tatcatcata atattcataa tatccagcat aacatgtgtc tgcattactc
     7261 ctttttatac catttaacat tgcttcaata taattagctg caacaaaatc atcagaatca
     7321 ataaaagcaa catattttcc ttttacatgt tcaagtcctg tatttcttgc caatcccaag
     7381 ccagcattat ttttatgaat aatacgaatc cttgaatcct ttaatgcata gtcttcacat
     7441 attttattag atgaatcctt agatccatca tccacaatta taatatctag gttcttatat
     7501 gtttgattac acactgattc caaacaactt cctacatact tttcaacatt atatacaggt
     7561 atgattacac taattagatc actcgaattc atccttctat cctttcactc acgttttctc
     7621 aattagtcaa tgacggcatc aacgattttc ttaaattctc tgaaaaaaat agactctgat
     7681 cttgatgatg tgaatgacat actatcttct tctagaaaaa ttatttcctt taaattattt
     7741 attaaatcaa taatatgatc ctcgtaagta tgctttccct tggaataatg aaaatatact
     7801 tttgtattac acaatattgc aattttattt cttaatatga ttatttcact cattcattac
     7861 tgccacatat acacaactta taaccacccc ctgcaaagtt tagggtaagt tatcagataa
     7921 tatcttcctc ttcatcatcc agaatacaaa atctatttat ttatagaaat atcattaatg
     7981 tttttatata atgtaccgtg cttgtactct tgctctatta caagcactaa aacactacta
     8041 ttcatttgaa ttcttacacg gataaaaatt tacatcgtct atttcaatat tttttctctc
     8101 tattttattc acttaataag ttcattacat aatcgaaaca ttttttattt tccttgtcat
     8161 cactccttgc tccctttttg atacagattt aaaattctat caaagaaatt tccatcgtta
     8221 aatttcgtta ttgccaaatc cttcgaacaa cgtgaaaatt ttacgtattt atctttatca
     8281 ttgattttta gtattgcaga tgtcaacgct gatacgtcac caggttgaaa caaatatcca
     8341 ttttctccat cacgtactgc tgaagaaata tctcctacat ctgtagcaat tacaggcatt
     8401 ccataagaaa tcgcctctag aatagatata ggcaatccct cgttatagga cggtaaaatc
     8461 atcacatttg atcttctata gcactcctcc tttttttctc ccacaaccca acctgtaaat
     8521 tcaaaatacg attgcaaatt atattcttct atcaattctt tgagcgatat ttcttcatca
     8581 cctgctccag caatctcaaa aataaacttt ttttctggat attgttctgc aatgttttta
     8641 actgcttcta ccaaatcgtg tacacctttt cgctttatca aaactcccat aaacagaaat
     8701 cggcacattt ttgaattcca ctttatggta tattctggaa ctacaataga attttctaca
     8761 acaacagttt tacaatttgg ctcaatttct ttaattatct tcttccattt ttctccaagt
     8821 acaatcgtgg aatcagactc tcttaacagc aagcgaatct gattttgttt ttttacagaa
     8881 acactatcat accatttttt aaattccgat ccatgaagat ggatgattac aggaatattt
     8941 aataatctaa accacttatt cagcacatat ttcctactaa aactccctct gtacgacatg
     9001 tgcatatgaa taatattagg tttactcaat aaagagatac ctataatttt agcaaatgca
     9061 tacataaaat ataaagattt aaatattgca tttccaccct tataactcgg tataaaagtc
     9121 atctcgatat tctttttttc ccaattaaaa ttgagcattt gtgtaattat acttgtaatt
     9181 ccacctttta ccgagctgtc attaccaatc ataagtactc tttgcataac gctttactcc
     9241 ttatatttat attttttcta ttaatacctc tatcgaatta ataatcgttt ccgtgttaga
     9301 tacatacttg ttaaacatcc gaactcttgt ttctttaacg gcatcttcta aattttctaa
     9361 ttcataacaa ggatagataa tattcatctc atcaaattgt ctaattaact gcagttggtg
     9421 atcatcgaca tgctcaccat acttagccaa tcgaggaaca gcaatgaccc ttttaccttt
     9481 ttttacggcc ccaattatag cacctgtacc tccatgagta atcaccattt ctgctttttg
     9541 catagtttca gtaaactcat ccctatttag aaatgcttta taggaataat ttagtggttt
     9601 ataatcacta taaccactct gtgcaaatgt actttcgatt attactcctt tttcaattaa
     9661 atcatcaata gcttttaaca atctatcaaa ttgaaatttt tgtgaaccta atgttataaa
     9721 tatcattttt ttctcctatt atttaatata tcccacccaa ataaatagca ttaggataaa
     9781 attgtagcat agtttcccac tgaacataaa attgatctgc aaaatgatac atcaattttc
     9841 ctgtctgagt agggctggtt acttttgcaa acgattcaat ataaatcagt ttttttccca
     9901 tcatttttgt tattaagcag atagggatca tagctaaaac acctgtacaa atcacgagat
     9961 ccggtttttc ctttatatat ataataattg atttgaataa attaataagt atagtacaca
    10021 aaaaaaactt ttcttttcta tttacttgtc tcatgtagta cgttttttcg ccttttgact
    10081 cagctgaata ttctgtctgt tctgttataa caaaactctc atacttctcc atcagtggtt
    10141 ttaacatcat taactgttca taatgccccc cagaggaagc cgcaaaacat atctttttgt
    10201 tcatattaac ctctctatca gtcacgctga aaaatatctc tagtgtaaac tttttcttta
    10261 atatcatcca agcaggtatc atatcgattg gcaataatag aatcacacat cgccttaaac
    10321 tgctctaaat cgttgataac cttacttccg aaaaaagtct ctccatcttt cagtgcaggc
    10381 tcataaatca ccacagtcgc acctttagct tttacgcgct tcataactcc ctggattgaa
    10441 ctctgacgaa aattatcact gttgctcttc atggtcagcc ggtatatacc gattaaaact
    10501 ggattttccc tgttgacatc atactgatta tattcagcgt agttgtaata gcccgccatc
    10561 tgaagaacac gatctgcgat gaaatcctta cgggtacgat tagattcaac gatagcctca
    10621 atcaaatttc caggaacatc tgcatagttt gccaacaact gcttggtatc ttttggcaag
    10681 caataacctc catatccaaa ggatggattg ttgtagtgag ttccaatacg aggatcgaga
    10741 caaacaccat tgattatctg ctgagtattc agccccttca tttcagcata agtgtctaat
    10801 tcattgaaat agctgactcg cagtgctaaa taggtatttg caaacagctt tactgcttcg
    10861 gcttcagtga atcccataaa aagagtatcc acgttctcct taattgcact tttctgcaga
    10921 agtgttgcaa aaacatgagc agcatttaac aagcgctcat cagtcatatc agtaccaaca
    10981 ataatacgag atggatacag attatcatat aaagccttgc tctcgcgcag aaactctgga
    11041 ctaaaaataa tgttcttact ccccgttgtc tcacggactg attcagtgta gccaacagga
    11101 attgtagact tgatgaccat gatggtatca ggattcacgt ccatcacaac ctcaataact
    11161 gactcaacag cagaagtatc gaaatgctga gtacgactgt catagttagt cggtgtagca
    11221 ataacaacga aatctgcgga tttataagcg ctccttgcat ccaaagtagc agttaagtca
    11281 agttcttttt ctgtaagata cttttcaata tattcatcct gaattggaga cttccgttta
    11341 ttaatcagtt caatcttttc agggataatg tctaccgcgg ttacctggtg atgctgcgcc
    11401 aatagtgtgg cgatactcaa accaacataa ccggtgccgg ctactgcgat gttgtacttt
    11461 ttcttcacaa tatctcctct ttcctctctt aagtatggag aatgttactc ctacggctat
    11521 tcatcatgtc aaaataacac agcttagaat gttgataaaa taattttcca gaatgtgata
    11581 aaatctcttt catttataaa ccacacatct aattttatct attctccaca agacatgtag
    11641 agtattcttt aataacaaga tacgacctta ctaaatactt caaaaaatta tcatatttaa
    11701 ttcataaata taaatcttca ttttaaatgt atgcccaaaa atttatctgt aatgcgtcat
    11761 caaaatctag attccaaatc tttatcaatt tttatccact cattctacca ctttagtaat
    11821 attttctatt tataacactt tcgtcctaca ttgatccccc cctctgaagt acagttttta
    11881 ctgtcttaaa taaaatttta aaatctaacc ccatactcca attttttata tattccgtgt
    11941 caagttttgc tacttcttca aaatcagtaa tatcacttct accacttacc tgccacattc
    12001 cagtaattcc tggcttgata gcaattcttg cacgatgatg ataatcatat tcttcatact
    12061 ctgatttaag gcatggtcga aatcctataa ggctcatctt tcctgttaga acgttgaaaa
    12121 attgagggaa ttcatctaaa gacgtggatc taataaattg accgattcct gtcttttttg
    12181 ttccatctgg aagtatttta tttccgataa ttcttggatc aaactccatc ttaaacataa
    12241 gattattgct catcttattt tctttcatta attctacttt tctttcttct gcatccatat
    12301 acatacttct aaacttatac atcttgaacg gtttaccatt ctgacctata cgttcttgtg
    12361 caaagaaaat tgggcctggt gaattaatat aaataagtgg agcaataaag ataaaaataa
    12421 ttcccgttgc aatacatcca acaatacctg caagaatatc aattgttcgt tttataaatg
    12481 cctgttgagt tgtcataaca ttgatacttg ttgtaagtac tgtatattct cctatacgac
    12541 caatagattg ttttcccaaa gcactatctg atacttttgc aagattcaaa tgtacagtaa
    12601 ggcccatttc agagcatcgt tcaataaatt ctttcataca cgaagtactt ttattcaaaa
    12661 taataaagac ttcatctacc catccctgac ataagtaatc cgctgcattt ttggaatcag
    12721 aaacaactgt cactccctca acatcttcac caatcatgct tttatctatg ataatgacac
    12781 catttatttt atagttatgc tctttcacat tacgaataac tacttcagca atactactag
    12841 atgtaactat aataagtgat ttgttcccct catttttcat tttatgtttc aagtaatgtt
    12901 tccataagag gcgagttata tatgtaatta caacgtataa acctcccatc aagaaaagaa
    12961 cgactctaga ataatcattt ccaccctgaa cactaatgag atacaaactt cctaacagca
    13021 ccaacataat cgcttgcttt acagaagaag caaattctat atagtaccct ctttttaata
    13081 cacctctata tgcttcaaac atgaaaataa ctaaaagatc tgccagttca atgaagactg
    13141 ccatgttttg atacattgga atcacataag gattccaatc tacctggcgt aagacatacg
    13201 atagataaaa agcaagctgg atacacaata aatctaaaat aataaaatca atatgtttaa
    13261 cccatcccct tgaacttttc ttgtacattc ctttttactc ccctcttttt aataaaataa
    13321 attgtattac ttgttttctc tcctaaatat ctttctgaag gattattgac tcgacaaatt
    13381 ggcttgtgga tattcttata tatagaacat ccgtaagcca acttaaaact ccttaacaaa
    13441 atttttttac tcatattgta gatcaaaaca agtatccaag aaatcatatt ttatttcttt
    13501 tttctgctta ctcaacaaca gctaaaagaa aaatcatcat aaacaaattc aactaagcca
    13561 acgatcccct tgtgaaatca agttttttct tgatttacga ataaagagct aaaataaacc
    13621 aaacttcttc ttttttacct cttcatagac tggataactg atttcatccc cgttgatcag
    13681 atcctttgcg acttgtttca tctgtactac tttttcctta ccaaagtcct ttgcaatctg
    13741 ctcatagcac tcttttaagt aaaaggtgcg cttagagacc ccatgggcat cagaagcagc
    13801 catctgcacg agattatgtt tgaccatcgt tttggctgtt ttctggatgt ctttgccaaa
    13861 taccccaaca taacttggcg cagtgagttg ggccaaacat cccatatcta ggaacggaag
    13921 caaatgattt gggtcttcgc gaaaatgcac gttgcgttct ggatggacga ttactggtgt
    13981 ctttcctagt tctaacaact caaaaaaaag ccgttctgca tataacggca cttccagtgt
    14041 cggaaattcg atcagaagat atgtatcatc taaatctgta aataatattt catctcggtg
    14101 gatgtcttcg atgagcgttc cggtaatacg tacttcttgt ccttccaata acgtcaaagg
    14161 caactgtctt ttctctaatt cggcttgtaa cgaagcgact agagaaatga cttgtgattt
    14221 ttcattgctg tattttccat tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg
    14281 ctgaatcgct ttttcagcca ttgcaatact tgcttctaag ttttctgccc cgtcatcgat
    14341 tcctggtagg atatggcaat gtagatcgat catttctctt gtctcctctc ttcttattcc
    14401 ttaatttccg tagtaataac cagcatcttt gctatgttct gctccgttgt acacaacacc
    14461 taagactcgt gcttgtacca tctccagcag ttcttttgct ttagtcaagg attctttacg
    14521 tgacatattt tcacgtacga ccaataaagt gccatccgtt tttaaagaca tgatctgtgc
    14581 atctgttacg gctactactg atggcatatc gaatatcacc acgtcaaata actgtcttgc
    14641 ttcggatagg atctgatcca ttcttggcga gcttaacaat tctgatgggt tgggtggctt
    14701 aggtccacta ggcaaaatcg acaagttctc gactggcgtt cgttggatca catctgctac
    14761 acttcctgat gaactaagtg ctgtacttaa tccactggca ttgttcagct ggaatgtctt
    14821 atagacgacg ggtttacgta aatctgcatc gactagtaga actcgttgac ctgatttagc
    14881 aaaaacgact gcaatgtttg ctgcagtggt ggatttccct tctccaggac ctgatgaagt
    14941 cacgacaatc gttttgattt gctgaccagc tgcggaggca aactggatat tcgtccggat
    15001 cgtacgatat tgttcggcga ttggtgaaga tgggtttgtt aatgtgatca aactgactgc
    15061 atgagtttgt gctgcttttt gtttctgtgt tcgtgccatt tttcttcctc ctagactctt
    15121 gagcggcttc tgcgtgatgg ggctgtttta tcgtttgtag cttttggtcg tgcaggagct
    15181 gttccttttt tacggatacc agcgttcaat tctttcgctg acatttctgg aactgttcct
    15241 aagattggga atcctaatgt atctgtcaca tatttttcat ctttcactgt ccgatcgagt
    15301 aattccaata agaatgccag acctacacct accatcattc ctaacattaa gccagtcatt
    15361 aaattcaatt tattatttgg cgaaacaggt gtcgtattgg caactgcacc tgaaatgatc
    15421 gagattttat caacattcat cacatctttt gcgttctctt ggaaaacttg tgaagttgtg
    15481 ttagcgatct gttgagcagc cactgcattc gtacttattg cctgaatcga gaacatttgt
    15541 gaattttctg cctgattaac tgagatggca tctttgattt gttcagcagt cattttcaca
    15601 ttgtcttctg tttctaaacg atctttgact tcattgagca ctaaatcact gacgatcata
    15661 tctttatacg tattgatcat ttgcaggttc gtattgacat cattgacatt tgcagtgtct
    15721 gattgtggca gtgtcacgat caattgtgct tgtgaactgt attttggtgt caagatgaaa
    15781 aatgttacga ttcctgagat ggccaagcca gcaaacattg agatgatgat tattaccaag
    15841 tgtttgcgta atgtttcaaa aatttctttc aagctgattg tctcttccat tttttctcca
    15901 ctttcctatt tattctctag attcatttga tcttttaatt cttttttcac acgatcaagt
    15961 tcttgatcac tgactttttg ataggagata ttatcttgca tgaagccttc tccttgcagc
    16021 tgatcttgct taatcgtacc aaatgcctcc cgataatcta aagcaatttt tttcatttca
    16081 tcaaagttca tgtctgtttt catattcgac tctagagcat tcaaaacgga ttgatagttg
    16141 ctgacaccgt ttagactcaa tacttttttc gctacgcctt ctacgatctt tctttggcgc
    16201 ccttgtctac cgtaatcacc atttgggtct tcataacgca tacgagaata agataatgcc
    16261 tgatccccgt tcaatgtaat tttaccgatt ggaaagtcat agccatcttg tgagaaagtg
    16321 aggtcattat tgacttcgat accaccaact gcatcaacga gttcctttag tcctttcata
    16381 ttgatagaga catagtgatc gattgggatg tctacatatt tttgtacggt atccatcgcc
    16441 atggcagctc cgccgaatgc gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg
    16501 atgtcgacat acgtatctcg aggaatgctg acgatcgttg tctgtttgtc ttcaggactg
    16561 actgtggcaa ccatcatcgt atctgatctt ccttgatcga cccgacctaa atctccagta
    16621 tcaatcccca tcaatagaac agagaaagat tcttgtttgt ccagatcaac tggtttcgtt
    16681 cgttctcctt gggttcgttc cactgattcg taagttttct caatagaatg tgagagatcc
    16741 agatataatt ttgctcctac tcctacaatt gctaatacta atacgataag aactgctaaa
    16801 ataatcaaaa atattttttt ccctcttgat tttcttcttc gtcttgccat agtttcctcc
    16861 gtttgctaac cttgcttgaa tcaacgtttt aactgtcaat agcttatatt gctatagccc
    16921 atttgctgag caacagtttg tgcttttgtt gcgtatgcgg ctcttatttt ttcattccgg
    16981 atctgctcta tttgactgag cgccgtcaaa tattgatcgt aagtagcggt agctgttact
    17041 tgaccgtctt tcagcatcgt atcgaatagc tgatctagtt gcgtgacttg tgcaatttgg
    17101 gtatcagcga atcctagata ttgcataacg actgttttcc aagcactatc cttaaaaaag
    17161 ctcatatttt cacgtacatg ggcgacatct gcacttgcca acttttcttt gatgatgaca
    17221 tcatccacag cttgctgcca gtttgatacg tttttagtaa ataatttatt gacagccgtc
    17281 tggatcttca gttttgattc tgcatcctct aactgcgtta agactgcttt cttttcttct
    17341 tggatcgcag cagcttcttt aggtaattct gattccttga tgtcataatc atctgccgaa
    17401 agtttgatag aagatagttt atgtgacaaa tttgaaactg tttcttctgt tatttcagga
    17461 tttaggaaaa ttttttcttt tgtactgtac agcttatcta cttcttttga taaagaggtc
    17521 acggtgtctt tttgattggt gatggcagat tctgcgtaat tgatccgttg atgatattgc
    17581 cgctgattat tgagatggta tccaaacaag caaatcacaa tagcgcctgt gcctaccgcc
    17641 acagtagcca ataacttgac gaacggcttt tccatagatt cactcctaaa tattgatgat
    17701 agataagtaa agatgtgatt cattcacatc agaatgagcg ctttttattt taaaagcata
    17761 attaaactaa tatattcata gaaatattta taaacaggtt ttccctatat aatcatacca
    17821 catacagttt ttttgactag ggaaattcct aatttaaaag taaataaaac agtaacaaga
    17881 ttataattaa tcgttttcat ttttatttcc tcacttttca tgagcgtttt gaaaattcga
    17941 acaaagaata aaataacaaa aattattaaa atagaattaa taacagctat ttataacaaa
    18001 aaaaacagag acacagcacg tttcgtgcat gctctctgtt tttcaaacaa aagactattt
    18061 tgtgatgatt tgatccaagc ttgctaatga caaagccatg gcagctattt gacttagttg
    18121 tgttagtcgg ttcttacgta ctgctggttc atcagccatg accatcgttt gatcaaaata
    18181 attttcgatc agtggacgaa gagcgacaag ctggtcgtag ttttcgtgga tcgtttggtc
    18241 agcaaaactt gctttcattg cttctactgc tttgtgtaat tctttctctg cgtcgttttc
    18301 gaacagctca gggtctactt ctgattgttt ttcaagctgc gcttttttcg ctaagttcac
    18361 gacacgtgtc aaagcttcca ttgaaggttt gaagtcttca tctgcaagat gttgtttgaa
    18421 caattgtgct gctgcaaata cttttgtcag atctttttgt tccgcattca aaacagcatc
    18481 gatgacatca tgacgaatat tcttcgttgt caataattgg cgcagacggc ctttgataaa
    18541 gtcgatcact tgttgttgcc cttctgataa accaatgccg aaacgatccg tatctttact
    18601 gataacatcg ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc
    18661 gatgatccga acgacaccgt atgcttggcg acgtaaggca taaggatcgt ttgacccgct
    18721 tggaatcatc ccaactgcaa agaaagaaaa gatgctatct aatttatctg caatcgctaa
    18781 aacagaacct actgtagact gaggtagctc tccttcactg gatataggca tgtaatgctc
    18841 acgaatcgct gtcgctacag caggtttctc gcctttcaga agagcatatt tttcgcccat
    18901 gatcccttgt agttcaggaa attcccctac catgttggtt actagatcaa atttatagat
    18961 ttcagcagct cgttgcaaat ccatcaattc ttcctcagat agtcctactt gttcgccaat
    19021 gatacgggca atgacttgta cccgttgcat tttctcatag atactaccga ttttttcatg
    19081 gaaagtaaca gtttttagtt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt
    19141 ataaaagaat tccgcatctt ctaaacgagc cgtcaatact ttttgattgc ctcgaaccac
    19201 atcttccaat tttacccgat caccattacg agcagaaata aaatgaggaa gcagcatacc
    19261 ttgatcattc cgtacctcga aataacgttg atgttctttc atagaagtga ctagtacttc
    19321 ttcaggaaca gaaagatatt tttcttcgaa gtcgccagaa aagacagttg gatattcgac
    19381 taaattgttg acttcttcta gtaaatcttc gtctagatca ataacccagt gatttttttc
    19441 tgctaattcg tttgcttgtg taacgatcat ttcttgtctt ttttgcggag acgcaatgac
    19501 gaattgttca ttcaattttt cttcatattc ttttgcatgc gcaaatgtca catcttcgcc
    19561 taagaaacga tgacctcgag aggctctgcc tgttttgaca tccaagattt cgaatgggat
    19621 tacttcgtca tctaacaatg ccacgatcca gtgaataggg cggatatatt caaaatcgta
    19681 ttctgcccaa tgcatcgtta cagggaaagt caaacttttg atcacccctg ctaagcctgt
    19741 caatacttct tttgcttcct taccatgagt gtatttcgtt acatagacat attccacacc
    19801 ttttaattct ctaaaagtga tagcgtctgt cgtcagacct tgtccgcgga caaatccttc
    19861 cgctgctttc gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac
    19921 ttcttcttca ctgtccgctt gtcgttctgg aatatttgtt actttgattg ctaaacggcg
    19981 cggtgtggaa aatgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc
    20041 tgttttttct tctaattgtt tcatgcttgg tgtgacaaca tgggcaggca tttcttccag
    20101 cccgatttca agtaataagt cttttgccat cttattcagc ctccttcacg gtttctggat
    20161 ttgctgtgtt tttattcaac aaagggaatc ctaatttttc gcgttctgcc acaaagattt
    20221 tggctacagc acgagccatg ttgcggatgc gagaaagata accggcacgc tctgttacgg
    20281 atactgcacc gcgggcgtct aataagttga atgtatggct acatttcaag atataatcat
    20341 acgccggatg aacaagattt tcatcgatac agcgtttagc ttctttttca aaacgatcaa
    20401 agttgcttag tagcaagtct tggtcgctta cttcaaaaga atatttggaa tgttcatatt
    20461 ctggttgttt gaagatctcg ccatatttca cgccatcagc ccattctaaa tcatagacac
    20521 tttcaacttc ttggatataa gaagcaagac gttctaatcc ataagtgatc tcagaagtta
    20581 ctggtttaca aggcagtcca ccaacttgct ggaagtacgt gaattgtgtg atttccattc
    20641 cgtcaagcca tacttcccaa cccacaccgg cacagcccat ggatgggttc tcccagttgt
    20701 cttcaacaaa acggatgtcg tgttctaatg gatcgattcc taaaaggcgc aagctttcta
    20761 aataaagctc ttgaatattc tcaggtgaag gtttcatcac cacttggaat tgatgatgct
    20821 gatacaaacg gtttgggttt tcaccataac gtccatcagc tggtcggcga gatggctcta
    20881 cgtaagcagc attccatggt tctggtccga ttgcccgtaa aaaggtatat gggctcatcg
    20941 tacctgctcc tttttctgtg tcataagctt gcatcagcat gcagccgttt gatgaccaaa
    21001 atttttgtaa tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc
    21061 ctttcaattt taaagcgttg ctgtcaaata agaatacagg aaagaaaacg acaaaaaatc
    21121 cctatgtctg attacagaca tagggacgaa taattcgcgg ttccacccta cttccgaata
    21181 aagattcggc agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc
    21241 ggctcccact ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac
    21301 gtattcattt tgcagttttc ttgtttctat aatagatcct atcactgaac gatctattta
    21361 atctatggtt aataatagac ggaactccct gtgtttgtca aatggttttg attttttgtt
    21421 gtatgtttta tttactattc ttctttttca ggtgtctctt tttctttttc agacatgtct
    21481 ttctctgacg tctttaatac gtgtgcccaa gacttcattt gattgatgaa ctttttgctt
    21541 ttcaaatgga taccgacata ttcatcatac agctggtcga tgagttcacg gattgcttgt
    21601 ttggtttctt ctttcagatg gattccacta attttatcat aagaaatagc tgaaaacatc
    21661 cgaacgaaat aaacagctcg aggatctgca tgataacggt gatcgtccat cgaccaatgt
    21721 ctttcgcata tcacaccacc atatttggaa gaatagtcaa attttccttt tgtttccccg
    21781 catacgacgc agtgtgtcca gattggtgca attccgaagc gttgcagtat ttggacttca
    21841 aaaatattcg tgatgatttc tgcatcgtct ccttggtcca tccgttgcag tgcttgttgc
    21901 gtaaactgga acaaatgcgg gtcgtactga tgatcttcga ttgccgcatc tactagattc
    21961 aataaatatg tgccatagcc gctcaaaaag atgtcttctt ggatgtgtcg aaacgcatgg
    22021 acatctttga cactgcttaa aaatgacaaa ccttcacttc gaaaattccc gatataaact
    22081 gcttcagtaa aagggagcaa agccggagct aagggctgat tttgccgatg gatctctttg
    22141 atataaaaca tcattttgcc agaagattct gtaaagattt tgactaattt atctttttct
    22201 ttgtaattac ggctagataa aatcaagcct tttgattctg caagctggct catattctcc
    22261 ctcctaaaaa ataaatctgg aaaagcagat tctgccgatc cagatcattt atgttgcgat
    22321 cttccgcagc ttttctcaac acgcggattt ttattcagtt tatttctata cctaatttac
    22381 aagaagctca gaatatcagt attcgtcttt tcggtagccg aagtcttgca gatagacttt
    22441 tttgtctcgc cagtcttttt gaactttgac ccaaagttct agaaagactt tgtctcctaa
    22501 aagattttcg atatcttttc ttgctttcgt tcctacttgc ttcagcattt ttccgccttt
    22561 gccgataatg atcccttttt ggctatctct ttcgacaata atcgtagcct ggatatggat
    22621 tttatcattc tcgtttcgct tcatcgaatc aacaacgact gcgacagaat gaggcacttc
    22681 gtctcgtgtt aaaaacaaga ctttctcacg gatcaattca gaaacgatga agtattcagg
    22741 atgatcagtc acttgatcat ctggaaaata ttgtgggccc tctggcattt cactgactaa
    22801 tacatccatc aaacgttcga cgttgtttcc ttctgtagca gagattggaa tcacttgtgc
    22861 aaaaggcatc tgcgttgaat aatcttcgat gattcccaac aactcgtctg gatggatttt
    22921 gtcgatttta ttgataatca gataaacagg agaatgcacg ttttttagac gttcaataat
    22981 aaaatcgtct ccccgaccgc gtttttgatc agcactaatc ataaataaga ccgcgtccac
    23041 ttctttcaac gcactgtaag ctgcttctac cataaaatct cctaaacgat gctttggttt
    23101 atggattcct ggtgtatcaa taaaaatcaa ctgagcttct ggtgtagtgt atactccttg
    23161 gattttattt ctagttgtct gtgccttgtc actcatgatg gcaatttttt gacccacgat
    23221 tcgattcaat aatgtggatt tccctacatt tgggcggccg acgattgcga caaaccctga
    23281 tttatgttct ggcatatatg ttctcctttt taattaaaca acaaatgata tattttaggg
    23341 acaaataaaa tcaacccaac gatgacggta aaacaagcgg tcactaaaac tgctccagct
    23401 gccatatctt tgattttttt cccaatcgga tgaaaatgaa aatccgtaaa catgtccacg
    23461 acattttcaa aaacagtatt gatgatctct actatccata cgagaaaaac agctaaaagc
    23521 agccataacc attctatccg atcaagttgg aaaatcacgc ccataaacaa agcgaggatt
    23581 ccgaacgcaa catgtttcct catgttgcgt tcttcctcaa aaacagtttt gactccttgg
    23641 acggcaaatt ccacggacat caggaaatgt ttgtttttct ctacgttttt atcttttaag
    23701 tccataggca tctaagatct ctttctgtaa accaaacatt tctttctcat cctctggtgt
    23761 catatggtca tacccgttga tatgcaagaa tccatgaacc gctaaaaagc caagttcccg
    23821 atcatacgaa tggccgtaag cttctgcttg ctctgcagca cgttcagtag agatcatgat
    23881 gtctcccagt acacgaggca atggattctc tccatcttca aaaatcactg ggatttcgtc
    23941 ttcgccctct tcttccaagg caaaactgat gacgtctgtc ggtgcatctt ttccacggta
    24001 atcacgattg attacttgga ttgctgcatt gtccatgaaa gtaacagaca tttctgtttc
    24061 ttctggcaac tctaaatatg atgctgcaaa ttgcagcaag tcgtcgattt ctttcatttt
    24121 ctcttctgag acttggtttg tttcatccat gaaggtaata tccattatac atcgcttctt
    24181 tctattcttc ttgctattct tcttgttctt cttttatctt ctcaacttcc gatttcattt
    24241 taggatattt gatccgagaa tggaaggtac tgcttaaacc gctgatcaat gatttttcta
    24301 ctttacggat ttcttttaac gtcaatccac tgtcgtccaa ttgtccatcg gaaatacgct
    24361 cttcaataag attatggaca aaactttcga ttttttcgct cgttggatgg tccattgccc
    24421 gtactgccgc ttcacaactg tcagctatat tgacgattcc tgcttcacgg ctttgaggtt
    24481 ttggtccagg ataacggaat tgctcttccg taacttccgg gttgcgttcc tttgccttaa
    24541 cgtagaaata acgcatcagc gtggttccat gatgctgtct acaaatatcg atcaccattt
    24601 gcggcatatt ataatcttcc aatattttgg caccatctgt cacatgtcca aagataatat
    24661 gtttactgtc ttctggcaat aagaaattgt gcggattttc cgctcctgaa ggcaagttct
    24721 caacgaaaaa gctggcgtgt ttgatcttgc cgatatcatg ataatagcaa gctactcgag
    24781 ttagtaaaga acgtccaccg atttctgcta cagcattagc acttagattg gctaccatca
    24841 tgctatggtg atacgtacct ggtgcttctt ccagcaactg tttcaataaa ggatgattgg
    24901 gattgctgag ttcatttaat acgatcacac tatcatcatt gaccatcaat tcaatatagg
    24961 ggtgcagacc tatcgtcagc aagaatgaaa acagtgcacc tgtgaatccg cagactagcg
    25021 ttaaccaagt cgttgcatcc ccaaagctca ttccttggta gataatcaaa atgacatcca
    25081 tcatcacagg aaaaataatg acccacatag cagcggataa cccttgttct gagatccgtt
    25141 tgcgtctgac gatcgttgcc atgagaccag aaaatacata ggacatcaaa ataactgtca
    25201 gaaagttcgt gccaatcgaa tcataaaaaa tgaaaagtgc ggatactgct tgaaaaatac
    25261 cagccatgat ccctgctctt cgattcaaga aaaagcttaa aacaagtgga gcaaaagccg
    25321 cggggtaaaa cagcggaata taagcagctt gttctgtttg gaagagttgg aaaaatttca
    25381 ttagtacgac gcttactgac attgccgtca cgtagaaaag tacgtatttt gttctttctc
    25441 ctgcattttg gaattgaagt gaattataaa tcagtacacc gatttgaagc aaaacagcta
    25501 aaaccatggc aatgagtggg aaaatagaag tcgtttgatt cgtcaatcct agtaattcca
    25561 attttttcat cgctgaagca tcaatttgat ttccttcgcg aacaataatt tctccttgga
    25621 agatcatcac tggttgtaca gcttctcgtg cagattgttt taattcttct gtctttttct
    25681 cattcaatac atcattaact acgatgcctt gatccactaa gtagcgaatc atttgttgct
    25741 gctctgttgt gacattcaaa tattgaatct gatcttctgc ttcttgttta aaagcttcca
    25801 attcgctttc acgaacttgt ttactcatct gttcgtcaat gagcttcaaa ctttcatccc
    25861 taactgtgtg aatttccgtt tcagtcattt caaatatcgt cgaataaaaa ttgttcggca
    25921 gtttttggta aaacgcaacg ttttctgcat tcacgttttc aaaatttttc ttcagtgtag
    25981 caatccgctc ttctgtagta ggtttcggga tattttcatc ttccttcgcc ttatctacct
    26041 tgccattata agatttgttg atggtatcat tcgtcttatc gatcaattcg aataattgct
    26101 tgatccgatt gttctggtca tctgccagat ctttttgata agtatattct ggagtcactg
    26161 cttctgctgc cagttttctt ctttgttcgg tttcttccgt gttctcaatc gttttattcg
    26221 cacggatact ctcttctgct acttggcctt ctctgaaatc aatggccttt tgtttgacac
    26281 tactaaatgt aacgccgcag acaatgataa aaaacaagaa cagaaggaaa ggcaatgctg
    26341 cctttccgag tttttcttgt agtttctgca gtgttttatt caacatgtgc ttatcctcct
    26401 aagcaaagct tattcttgat gaagatccgc cttttcataa gctttgatga tttcggcaac
    26461 tacagggtgc cgaacaacgt cacttgcttc aaaattaaca aaatcgattt ttttgatttg
    26521 ctttaatgtt cgttctgcat gaacaagacc acttgtcgtc ccacgaggca agtctatctg
    26581 acttgtatcc ccattgacaa tcatttttga ttgataaccg agacgggtca agaacatttt
    26641 catctgagcg actgtcgtgt tttgtgcttc gtccaagata acgaatgcat catccaatgt
    26701 ccgcccccgc atgtaagcta aaggtgcgat ttcgatcacg cctcgttcca tcaatcggtt
    26761 cgtatgatcc aatccaaaaa tttggtataa agcatcataa acaggccgca gataaggatc
    26821 cactttttct ttcagatccc cgggtagaaa gcctaggttt tcccctgcct ccacagcggg
    26881 ccgcgtcaaa atgatttttt gcacttcgcc tttttttagg gcagcaattg ctaatactac
    26941 ggcaagaaac gttttccctg ttccggctgg ccctacacca aatacgatgt catgtttggc
    27001 taccgattca acgtatttct tttgaccgct gttttttacc cgtataggtt ttccattacg
    27061 gtctttgacg atctcttcct catacatctc aacaaaataa tctaatgtac ctttttgccc
    27121 cattttcaat gccgtcacaa catctggcgt acttacgtgg attcctcgtt tgatcaattc
    27181 ctgtaatgtc cgaatgactg aggcagccaa ggaaatctgg gattgctcac caatgatctg
    27241 aatgatttct ccacgtgtat ggatggttgt ttccgttgtt tcctcgatca gttttatatg
    27301 cttgtcgtgt gcacctaaaa gcatgctgac atcatcagca gctgtcaatc taatctctaa
    27361 agaactagac tcttccgtca aaaatagaca tccccttttt ttcgattcat tctttcctat
    27421 tttaccatac tcagaagaaa ctatatagca aataagaaaa ggttgtaaaa gagtcgctta
    27481 accatacgta ccaagaaacg ttaaaaaata gccgtttcca ttcgctaaag gtctcgtttt
    27541 gataaaaaga agctgagaat gtaagttata tgaaagtgga gaacaaaata tttcgtttca
    27601 ttcaacaagt attctctagg tatttttcct gtttcaaaaa aagaaaacaa aatagttaaa
    27661 atattcttgc atttcaaaaa aaacaggtgt atttttacgt caacaaataa acatacagca
    27721 aagcgtcgag gaaaacagta ggctattcaa cgatttattc ccagtgagtc atgtaagtga
    27781 gaaatgacaa taaatcgaat agacgaacat catttccgag tcaatgtcga aaacgtagat
    27841 cattgtggct gcctccatta tcaggctagc ggataacttt atcaaaaagc gtccgtgaat
    27901 gaagaagctt ttttaagcga aaaaaggtgg taccgttcta gaatctagag cccttttccc
    27961 aagtggaaaa gggctttttt ttattttaag gtggtggttt tcatggaatc tgatattagt
    28021 ggcattacta attgctgcaa cgtttatatc gtcaatcaaa atgataatta ttggaggaaa
    28081 aatttatgga agaaacaaaa acttattcat ggaaacaact attgatggtc agttcactta
    28141 tcttcgggat gttcttcggg tcaggtaatc taatctttcc tgtccactta ggtcaaatgg
    28201 ctggcgcaaa ctgggtcagt gctggcgtag gatttgctat ctcaggtgct cttttcccat
    28261 tactagctat cttagctgta gtagtgacaa aaagtgatgg tctgtatgac ttagctaaac
    28321 ctgtcggaaa atggtatgct gccttgttct taatccttgt tcatttgacg atcggaccat
    28381 ttttcggtac accacggaca gctgctactg cttttgaaat ggctgctaaa ccatttttgc
    28441 ctgaaaaata taattcatta ggtatgtttt tattctctgc tcttttcttt ttacttgctt
    28501 atttccttac tgtccatcca acacggttgg tcaaatacgt cggaaagtat ctaaactcct
    28561 tgtttcttgc attattagca atcatttttg tcgtttcttt attccatcca atgggaaatc
    28621 taaatcaatc tgctgcagat acttatcaaa caaacgcttt aacaaatggt gtcctagaag
    28681 gttataacac ggttgatgca gtagctttgc ttgccttaag tgtcactttt gtccatgcag
    28741 tccgtgacct tggtttcaaa gataaaaaag tcacagaatt aacggcaaaa gctggtagcc
    28801 tgagcgttgc gctagaagtc atgatctatt ttggacttgt tcttctcggt gcgatgagtt
    28861 tgaataagat gtctttatca gaaaatggcg gaactgcact ttctcagatc actaattttt
    28921 atttaggtaa cttcggcgct agcttcttag gagtgatggt cacattaggt gtcttcacta
    28981 ccgctatggg actcgttgtt tcttttgcgc aagatttcca taaactgttc cctaaagtca
    29041 gttatatgac atggctgcgg ttaacgactt tcgtctcttt tgtcgttgca aatgctggtt
    29101 tagacaatat catccaatgg tctttaccag tattgatgct tttgtatcca ttgtcgcttg
    29161 cgttgatctt gctttcctta acagcaaaat ttttccaaaa gacaccattt gtctatcaag
    29221 taacgatgtt gtttgctgct gttcctgccg tattggatat gctggcaaat tcgcctgctt
    29281 tagtgagtca acaaagagtt gtagcaagca tgctcgaatt ttatcatcat cacgttcctt
    29341 ttgctgcatt aggacttggc tggatggtcc caacgttact tggttacgct ggcagcctac
    29401 tgttttatta cgcctatcgc ctatcagggt ataaacaaga agcaaatgaa ctaccagaag
    29461 aataaccaaa tcaaacacaa tttaacataa gaaatattaa acaggaaaaa accagctaga
    29521 tgaaagaatc ctattctttc gtctagctat tttttagttc tcttttttac gcgcacaaaa
    29581 aaactgcgga caatgattgt ccgcagttta aa
//
LOCUS       DAFEHS010000028        27943 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFEHS010000028
VERSION     DAFEHS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.028"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            7..849
                     /locus_tag="LP327_001345"
     CDS             7..849
                     /locus_tag="LP327_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL1725471.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            849..1796
                     /locus_tag="LP327_001346"
     CDS             849..1796
                     /locus_tag="LP327_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL1725472.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1811..2044
                     /locus_tag="LP327_001347"
     CDS             1811..2044
                     /locus_tag="LP327_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBL1725473.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2375..2959
                     /locus_tag="LP327_001348"
     CDS             2375..2959
                     /locus_tag="LP327_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL1725474.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3090..3758
                     /locus_tag="LP327_001349"
     CDS             3090..3758
                     /locus_tag="LP327_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL1725475.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3769..5184
                     /locus_tag="LP327_001350"
     CDS             3769..5184
                     /locus_tag="LP327_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL1725476.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            5202..5771
                     /locus_tag="LP327_001351"
     CDS             5202..5771
                     /locus_tag="LP327_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBL1725477.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5881..7635
                     /locus_tag="LP327_001352"
     CDS             5881..7635
                     /locus_tag="LP327_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725478.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7595..9361
                     /locus_tag="LP327_001353"
     CDS             7595..9361
                     /locus_tag="LP327_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725479.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVTMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            9884..10153
                     /gene="rpsN"
                     /locus_tag="LP327_001354"
     CDS             9884..10153
                     /gene="rpsN"
                     /locus_tag="LP327_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL1725480.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10168..10347
                     /gene="rpmF"
                     /locus_tag="LP327_001355"
     CDS             10168..10347
                     /gene="rpmF"
                     /locus_tag="LP327_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL1725481.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10379..10756
                     /locus_tag="LP327_001356"
     CDS             10379..10756
                     /locus_tag="LP327_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725482.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10777..10926
                     /gene="rpmG"
                     /locus_tag="LP327_001357"
     CDS             10777..10926
                     /gene="rpmG"
                     /locus_tag="LP327_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL1725483.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10950..11933
                     /locus_tag="LP327_001358"
     CDS             10950..11933
                     /locus_tag="LP327_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL1725484.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11963..12085
                     /locus_tag="LP327_001359"
     CDS             11963..12085
                     /locus_tag="LP327_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL1725485.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12103..13626
                     /locus_tag="LP327_001360"
     CDS             12103..13626
                     /locus_tag="LP327_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL1725486.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIIGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSKHEIAQEMMAH"
     gene            13650..13889
                     /locus_tag="LP327_001361"
     CDS             13650..13889
                     /locus_tag="LP327_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1725487.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14082..14543)
                     /locus_tag="LP327_001362"
     CDS             complement(14082..14543)
                     /locus_tag="LP327_001362"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL1725488.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14738..14956)
                     /locus_tag="LP327_001363"
     CDS             complement(14738..14956)
                     /locus_tag="LP327_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1725489.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15074..16690
                     /locus_tag="LP327_001364"
     CDS             15074..16690
                     /locus_tag="LP327_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL1725490.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16931..18271)
                     /locus_tag="LP327_001365"
                     /pseudo
     CDS             complement(16931..18271)
                     /locus_tag="LP327_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18547..18702)
                     /locus_tag="LP327_001366"
     CDS             complement(18547..18702)
                     /locus_tag="LP327_001366"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL1725491.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(18894..19088)
                     /locus_tag="LP327_001367"
     CDS             complement(18894..19088)
                     /locus_tag="LP327_001367"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL1725492.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(18998..19330)
                     /locus_tag="LP327_001368"
     CDS             complement(18998..19330)
                     /locus_tag="LP327_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL1725493.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRLAISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19844..20035)
                     /locus_tag="LP327_001369"
     CDS             complement(19844..20035)
                     /locus_tag="LP327_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1725494.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20129..20515)
                     /locus_tag="LP327_001370"
     CDS             complement(20129..20515)
                     /locus_tag="LP327_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL1725495.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21209..22228
                     /locus_tag="LP327_001371"
                     /pseudo
     CDS             21209..22228
                     /locus_tag="LP327_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
     gene            complement(22305..23063)
                     /gene="istB"
                     /locus_tag="LP327_001372"
     CDS             complement(22305..23063)
                     /gene="istB"
                     /locus_tag="LP327_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBL1725496.1"
                     /translation="MTTNHQKVLTNLDELGLLKMKEYYPHYLDSVRNQTISLTDALYN
                     LTQKQLDYQKEMIDKRAIKLARFPTIKSLEEFDFEFQPSINKHDILQLKSFHFLESST
                     NICFLGNSGVGKTHLATAIGIEACIQGVKTRFILFNDLIEKLTRASQRGTLVRTLKIF
                     TNVPLLIIDEIGYTPISKTQADWFYQLMARRYERASTIITTNIPFSMWAKNFDNTTAS
                     AAILDRLIHHSKVFKITGNSYRLKNYHLDKETIG"
     gene            complement(23079..24305)
                     /gene="istA"
                     /locus_tag="LP327_001373"
     CDS             complement(23079..24305)
                     /gene="istA"
                     /locus_tag="LP327_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBL1725497.1"
                     /translation="MRKDVYEGVRFFMINDIKPNFNELGRQYNCDPRTVKKYYLEGSE
                     NSVKKDKDKPKRPSKLDPFKEIIDQKLELNCSAMAIFKFLKKIGYQGGYTIVKDYCRN
                     KKHTEIKKATLRVETVPGTAAQVDWKEDMTLHDKFGKSYTFNLFLYVLHFSKLKFITL
                     TWDRKQDTLFSCLYEAFNHTGGVPKEIWFDNMKTVVDRARTQYRKVVFNTRFYEFSKD
                     AGFEAIACRSYRPQTKGSVESLAKLVERLKPYDYEFYDAVDLISLVNTLCYDLNYEEI
                     SQSTYQRPVDLWEREEKGKLHPVNEALLTPYFEDNIVRIVTNESMVNFRKSKYSVPTK
                     YIGCEVEIEIDPTEKFIEIFYQGEVVQSHELTGKRLNYKETDMFEILKSDVMKHKDDE
                     EIYEYIQRTLTQYDEL"
     gene            24413..24604
                     /locus_tag="LP327_001374"
                     /pseudo
     CDS             24413..24604
                     /locus_tag="LP327_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            24621..25055
                     /locus_tag="LP327_001375"
     CDS             24621..25055
                     /locus_tag="LP327_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725498.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            25672..>27943
                     /gene="esp"
                     /locus_tag="LP327_001376"
     CDS             25672..>27943
                     /gene="esp"
                     /locus_tag="LP327_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBL1725499.1"
                     /translation="MISKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVKAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTDNWEIRLQLDERLAKYVENIQVDSKKVAGISYRRTF
                     VRINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKNLDEIFQEIGED
                     NLKSDRLMYRIYLVSHQDNDKIVPGIESTGYFITDQDNFYNKLDVSENNPDQFKHGSV
                     STKYEKPNVQTKDGSGSTGANGAIILDHKLTKEKNFSYSVSATGTPWYANYKIDERLV
                     PYVAGIQMHMVQADRVVYDVSFESGTKVADLAIERREGHENYGVGSITNNDLTKLIDF
                     SNASPRPVVIRYVLQLTEPLDEILAEMKTAAQVEGNEPFGEDFIFDSWLSDTNKKLIQ
                     NTYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAG
                     TGHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSNKLIAKDELKVQIVS
                     PGFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTS
                     NVADSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTIQSGKPTFD
                     KPDVPLANDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGETEPITGTIPIKIVYQ
                     DGSVGTTDLAVTVSKDI"
BASE COUNT         9534 a         4069 c         5183 g         9157 t
ORIGIN      
        1 aataatatgc ttcaaaattt tattaatggt ctgtatgact ttcacttttt acagaatgct
       61 ctaattacat ctgtagttat agggattgtt tctggaacag taggttgttt tattatttta
      121 agaggaatgt ctcttatggg tgatgctatt tctcatgctg ttttgccagg agtagcaatt
      181 tcttatattt taggtattaa tttttttatt ggtgcgattg tatttggtct attagcttct
      241 atcattatta cttttataaa aagcaatagt gtaataaaag gagatacagc aattggtatt
      301 actttcagtt cttttttagc attaggagtt atccttatag gagttgcaaa tagctcaacg
      361 gatttatttc atatcttatt tggaaatatt ttagctgtac aagatattga taagtggatt
      421 acaattggcg tttctgtttt agtgttggtt acagttgttc tattttttaa agaattatta
      481 atcacttctt ttgatccatt aatggcaaaa gcaatgggga tgaatgtcag tttttatcat
      541 tatctattaa tgattctatt aactttagta gcagtaacag ctatgcaaag tgtgggaact
      601 atattaattg ttgcaatgtt aattacacca gctgctacag catatttata tgcaaacagc
      661 ttaaaaacta tgatatttat ttcggcgaca gtcggagcgt gctcatcatt gctaggtttg
      721 ttcattggtt atagttttaa catagcagca ggatcaagta ttgttttaac agcggcattc
      781 ttatttgtaa ttagttttct aatctcacca aaacaaaaat ttttaagaaa gaaggagaga
      841 aagttatgat gagaaaatgg aaagcagtac tgggaagtct ggggattttg attgctcttt
      901 ttatattcgg ggcatgttca acaaatagta aagacaaaga tacagtggct tcgaacgaaa
      961 aattaaaggt agtagttact aattcgattt tagcagatat tactgaaaat atagcaaaag
     1021 ataaaattga tttacacagt atcgtaccta ttgggaaaga tccccacgaa tatgaacctt
     1081 tgcctgaaga tgttcaaaaa acttcaaaag cagatttgat tttttataac ggtgttaact
     1141 tggaaactgg aggaaatgct tggtttacaa aattagtaaa aaatgcgaac aaagaggaaa
     1201 acaaagacta ttttgcagca agtgatggca tagatgttat ttacttagag ggtcagagtg
     1261 agaaagggaa ggaagatccc catgcttggt taaatttaga aaacggtatt atttacgcta
     1321 aaaatattga aaaacagtta gcggaaaaag atcctgataa taaaaaattc tataaagaaa
     1381 atctagataa gtatattgaa aaattggatt ctctagacaa agaagctaaa tctaaatttg
     1441 cttcaattcc gaatgataaa aaaatgattg ttacaagtga aggatgcttt aaatatttct
     1501 cgaaagcgta taatgtgcct tctgcttaca tttgggaaat caacactgaa gaagaaggaa
     1561 caccagatca aataaaacac ttagttgaaa aattacgcac aacaaaagtt ccctccttat
     1621 tcgtagaaag cagtgtggac gatagaccaa tgaaaacagt atcaaaagat accaatattc
     1681 ctatctattc aacgattttt actgattcaa ttgcagaaaa aggacaagat ggtgatagtt
     1741 actatgcgat gatgaaatgg aacctggata aaattgctga aggcctttcg aaataatttg
     1801 gagggtaggt atggtacaaa ttaaaacgac tactatacta atttatttac tagcaattca
     1861 ggaattatca aaaagaaaaa agaaattaaa aaataatgat ttagcaaaag ttctaagtgt
     1921 tagtccagca tctgtaagtg agatgctggc taaacttaga tatgaggact atttagatat
     1981 aggatttcaa ttaactgata aagggaaaaa atttattgac gattacaaaa aaggttttaa
     2041 gtaaaagact ttatttttag ggaggataag aaaatggcta ttggagtgat tatgatagga
     2101 cttaccctaa tagctggata taattttttt aaatctttct cggattattt tagaaaaggt
     2161 gaatgattta gatgagtcta ttaattcttg taggattgct ttttgtatca gttattttgc
     2221 tctttctcat tagtttagct ctaaggaatg agttcagtaa tagacgttaa cgattgaaac
     2281 ttcaagtcct gtgtaagata atatagttaa ttcatcttat taggaggaag tacccataga
     2341 aaccagagat tatattacaa ctcaccaaaa attaatagaa agtggactaa gccattttga
     2401 aaaagaagga tttcagcaaa ctaagataag gcaactgtgc ttgaccgcag gcgtaactac
     2461 gggtgctttt tataaacatt ttacttctaa ggaagcgtta tttgaagaaa ttattcagcc
     2521 gcatattaat gcgcttatta atatgtataa tgaagagttt accaaatacc tcttaaactt
     2581 agaaaatata gatatagaga aaagttggag agagaacttc aatgaattag atccatttat
     2641 taattatatt tttgagaata aacgtatatt cgatttggtt ttattccaat cagctggaac
     2701 caaatatcaa gatattgttg aaaaaattac atcatttgtg acaaaaaaga cagtctgttg
     2761 tctagataag ttaaagacta tcggaatgat caatcaaaat ttagtcttta atgaagaaga
     2821 aatccacttc tacatttact cattctacgc tacattctat gatatattga agcatagcta
     2881 ttcaaaagaa aagactatgc tattaaccag aagattgtat caattttctg ttcctgggtg
     2941 gattgaatta ctgagttaat taaaaaaaag cttgcattga gaattgttct cagttatgct
     3001 ttttgataag atcgcactgt tatctgaata acggtgcatt tttgtcagct taagataaca
     3061 gtgcgatctt attttttgga ggtgaaagag tgtcaaaaat caatccgtta accttgatta
     3121 ctttaaatat tttttttccg gtcattattt ttttaggaaa gggaaatata tttcaagtgg
     3181 ggtgtttcat tattgctaca gttttagtat tgatgtttcg taaaataaag acatgtattt
     3241 atttcatctt gatttattca gttctgtttt tagtaatgaa aattattgat gctatcaaga
     3301 tagagttttt tactatattt tttgggacat ccttatatat tgtatttcga atgttgccgg
     3361 tcgtaatgat ctcatggttg ttggttagtg tctatactag caatgaatta ttatcctctt
     3421 tagaaaaaat acatttaccg aaaaaaataa tgttaagtat tacagtagtg atcagattct
     3481 ttccgactta tcgttctgaa ataagaatga ttaaagaatc tttaaaaatg agaaacattc
     3541 gcttgtcttt ttttcagcct ctgcaatatt tggaatttct gttagttccc gttcttatac
     3601 gtgcaactat gattgctgaa gaaatgaccg ctaatgcaat aacaaagggg atagaatctc
     3661 ctgtgaaaag aagttcttac tataaaacaa acatgagctt attagactat atcatcctgt
     3721 tttccgttac tatttttttc ttaagtattt tgatttgagg tgaaagaatt gtttgaattt
     3781 aacaatgttt ctttttcata taaggatgtt aaggaacaag cgcttgttga ccttaatttt
     3841 tcaataaaaa aaggcagtct tacattgatt actggttctt ctgggagtgg gaaatccaca
     3901 ctattaaagt taatgaatgg acttattcca agactttatg acggaaaatt ggttggggaa
     3961 attttatttg aaaataactc gctacaatcc ttaacacaag aaagtatatc aaaaaatatt
     4021 ggctacgtaa gtcaagatcc tagaggacaa ttctttacaa caaatacaac gagtgagtta
     4081 gtattttcga tggaaaactt tggatttaca aaagagcata tggataaaag aatcaatgaa
     4141 ttagctgaat tattagatct ttcaaatata ttagataaaa acattttttc gctatcaagt
     4201 ggtgaacggc agaaaatttc tattggatgt agtttatcgc taaactctaa attaattata
     4261 ttggatgaac cgtcttcaaa tttggacttc aaagcgacta agaaacttgc tgacttactt
     4321 tgtgatttaa aaaataaagg atttactatt gttatagctg aacatcgttt ttactatttg
     4381 aaagaattaa ttgatcaagt tctatttgtt aaaaatagaa ctgtaaaaac tacaactatt
     4441 cagttgttaa aaactgaaaa caataacgca ttaaggaaat ttgacatttt taatagttcg
     4501 attgaagttc cctatatcga aaaggtaagt gcagatgtat taaaagttga agaaattact
     4561 tatgaaaata ttctaaaaga cataaatttt gatgtcaaag ttggagatgt tattggtttg
     4621 gtagggaaaa acggtgtggg gaaaacaacc ttactaagaa tactcatgaa gattattcgc
     4681 cctaccaaag gaaagataat cgaaaataaa aaaaatttgt catcgccgtt tttagtaatg
     4741 caagaaatgg attaccaatt tttcacagaa tcagtgagga gcgagttagc cctggggaat
     4801 gaaatagtta gtaaagatca gcaagaaaaa attttaaaaa aaatggaact ttataaaatg
     4861 aaagatcaat taccgtttga tttatccggt ggagaaaaac aaagattatt ggtttctata
     4921 gcttcattgt caaaaactaa tttatttcta tttgatgaac ctacaagtgg gttggattat
     4981 atcaatatgg aaaggattgc tgaccttatt aaagacttaa aagaaaaggg tgcagtagtt
     5041 atagccactc atgatccgga atttctttat aaaacatgca atagaattat ttatttaaaa
     5101 aatggtggaa taaaaaaaga tataaaactt agagtagaag attcaacttt gatagaacag
     5161 atttttaatg acataacgaa atagaattga ggagaatata tatgaaaaca aaagattata
     5221 tttttattgg tttagctaca gttatttccc tagtaattta ctttttttca ataatggtta
     5281 gtagtattgg tggggcgttt ggtcacagca tatccccagg gatttttgga ttattatctg
     5341 gaatcatatt tgtttatatt agctataact accctagaaa aggaattttt actatttaca
     5401 caattgtttt attgttattt tttacgttaa tgggcggggc gtacttacct tggtttatca
     5461 gttcgattag tatggcgatc ttagctgata ttattctatc tgtatttgga tatgatcgag
     5521 caattcctca agttgtgtct tgggttctaa tgcaactagg cagtgctgcc ggacagtgga
     5581 ttcccatatg gttctttaca gatcgttttc gtcaagactg gatagataga ggacaatctg
     5641 ctgcaacaat ggatgcaatg atccactacg cggtgggtat atggggaatt atttcagtgt
     5701 tagttgtagc aagtttatct atgattggtg tattgattgg aagaaaagtc ttaaaaaaat
     5761 acaaaaaata gaacgtagtt taccttctat ctgtttgtac atctctaatt tacgctatta
     5821 cggaaagcat taacagttgt aaacgaataa gagaagaaat tgaatgcggg ggaggaaaaa
     5881 atgaaagtat acaaaaaatt atttagttat gtccctaaaa tgaaatacta cggatggtta
     5941 gccactttat tttctgattt ttctgtcgta ttaacagtct atggatatta cagtatttac
     6001 aaatttcttc agtctctaat tattgtagga gatgaatatt tagctaagag ttatgcggtt
     6061 caaactgttg cttggttaac tttaggagct ttattttata ttttttctgg cttattttct
     6121 catatccttg ggtttcgctt agagactaat ttgagaaaaa agggaattag cggtttaaca
     6181 gaagcaagtt ttcgtttttt tgatttaaat tcttcgggat atattcgaaa gacaattgat
     6241 gacaacgcgg ctaaaacaca tatggcaatt gctcacttga ttccagatag tgctcaagcc
     6301 atagtaacac ctgttttatc agttgcctta ggctttttca ttaactttaa aattggattg
     6361 gttctaatcg ccatgttagt aattggtgta ttcttgttaa agaaaatgac agagaataca
     6421 aattttattc aaaaatatca agaatcgtta gatgtgttaa gctctgaaac ggtagaatat
     6481 atccgcggaa ttcaagttat aaagatattt ggtgctaaag taacatcatt taaagccttg
     6541 aataaagcaa ttaacgatta tgctaagttt gcatataagt attctttgag tgggaaaaca
     6601 ccttttgttc tttttcaatg gctatttttt ggcatagttt gcattttgat agttcctata
     6661 acctttttta taacttctct taccactccc aagatattag ctgttgactt gttgatgttg
     6721 ttttttttaa gtggaattat ttttgtttct ttcatgagaa taatgtatgt aagtatgcac
     6781 ttgtttgaag caagatatgc agtcgaaaca ctagactccc tttatttaga aatgaagcaa
     6841 gaccaactaa aatatgggaa cgagacacag attaaaaatt gtgacattgc atttgaaaat
     6901 gtcagctttt catacaaaga agagtgtgtt ttagaaaatt tcaacttagt tttagaacaa
     6961 aaaagaattt atgcattagt aggaaaatca ggaagcggaa agagtacgat agctaaacta
     7021 atttcaggtt tctacaatgt ggataaggga cagattaaaa ttggtggtaa gcctttaaat
     7081 cactattcaa aagacacaat tattaacaca attgcttttg tctttcaaga tacgaaacta
     7141 ttcaatcaga gtatttacga taacgtttct atagcaaatc ctcaagcgcc agcaagtaaa
     7201 gtatttgacg cgctaagatt agccggttgc gaatcgattt tagaaaaatt tcccgaaaga
     7261 gagcatacct taattggtag taaaggagtt tatctgtcag gaggagaaaa acaacgtata
     7321 gctattgcca gagcaatttt aaaagatgca aagattgtta ttatggatga agccagtgca
     7381 tctattgatc cagataatga gtatgaacta caaaaggctt ttcaatattt gatgaaagaa
     7441 aaaacagtta ttatgatagc tcatcgatta tccagcatta aatctgtaga tgaaattctt
     7501 gtcttgtcag aaggaaaaat agtagaacgt ggaaatcata atgaattaat ggctaaacat
     7561 cgagtgtata aacaattggt cactaattat gagaatgcaa atgattggag gcttgacaat
     7621 gaaaaatttt tataaaaaaa aatttgcttt aactgatcag ggcgcagaag ctttaactaa
     7681 agcttctatt tctagttttt ttgtatactg cattaatatg gtaccagcat tcattataat
     7741 gatgctgata gacgaattag ttttagaaaa tgcaaagccc cgttggcttt attttgcagt
     7801 atcgtttgtt actttgcttt tcatgtattg gttgttagat agggagtatg aaaacttata
     7861 taatagtact tataaagaaa gtgcgcattt aagagtgcaa attgcagacg atttgtcaaa
     7921 tttaccatta tcctattttt caaaacataa tttatcagat ttatctcaaa ctatcatgtc
     7981 tgacgttgaa ggtattgagc atgcgatgag tcatgcaata cctaaagccg gcggtatggc
     8041 tctgtttttc ccttttattt cagtgatgct tttggttggt aatgtcacaa tgggattagc
     8101 tgttattttg ccaacgttat ttagttttgt cttaatcttg ttatcaaaga aatcccaaac
     8161 gaaagccaat actaaatatt acgatacttt gagagaaaac tcggaagaat ttcaagaaac
     8221 tattgaattg cagcaagaga ttaatagctt taatctatct aaaaaagttc aagacagact
     8281 tttcaaaaaa atggaagaga gtgaaaggat tcatttaaag gtagaattaa gtactttttc
     8341 agtcatggcc ttatcctcta ttttctcata tgttagttta gcagtagtca ttctagtagg
     8401 tgttcactta ctgttaacgg gtgaagtgac tatactctac gtcgttggtt acttactagc
     8461 cgcaataaaa ataaaggatt ccttcgattc tatgaaagaa gcagtgcttg aaatatttta
     8521 tttagctcct aaaatacagc gaattagagc tatgaaggaa accagtatcc aagagggatc
     8581 agatagtcct ctgaaatctt ttgacattga gttgcgagat gtctctttct catatgataa
     8641 caatacccca attttagatc atatctcttt tacggctaaa caaggagaag tgactgcgtt
     8701 agtaggtgct agtggttccg ggaaaactag tatcttgaaa ttagtgtcta gactgtatga
     8761 ttatgatgaa ggctgtattt taattgatgg gtatgatatt aaacgtgtat ctccagcttc
     8821 tttgtttagc aaaatcgcta ttgtttttca agaggtaact ttgtttaata caagtatttt
     8881 ggaaaatata cgtattggaa atagtcaagc gtcagatgag gaagtgaaaa aagcagctcg
     8941 tttagctaac tgcgaagact ttattgaaaa actacctgac ggttatcata cattggtcgg
     9001 agaaaacgga agttcacttt ctggaggaga gcgacaacgc ctatccatag cacgggcttt
     9061 tttgaaaaat gcgccaattt taattttaga tgaaattact gccagtttag atgcggaaaa
     9121 tgaaaaacgt attcaggaaa gtttaaatcg tttaatacaa gataagacag tactaattat
     9181 atcgcatcgc ttgaagtcta ttgaaaaggt aaataaaatt gtagtgatgg atcaaggaaa
     9241 actagttgat caaggtactc ataatgagtt gtatcgaagg tcagaaatat acaaaaatct
     9301 cataaagaaa actaaactat ctgagaaatt cgtctatgaa aaagaagctc aatcacgatg
     9361 atgaaatagg ctttaaatta acctacaatt tttgggatat agttataatt ctttttcaaa
     9421 aagaataatt ttattaaaca gcatctaatt ttgaatacac gaaagaatca gtatgattga
     9481 attagttcac ttaatgtcta ttcttataat ataaggaata atcaaagttt taaaaaattg
     9541 gcgataaacc agttaaacat aaaagattga ataaaaagtt atagtttctt tcatattgcc
     9601 gttttaagta gaggaaaagc tacttaaaac ggtatttgtg tttgattgga ctaataaaag
     9661 tgaattctta tttttcagag cctaaactgc atattctttt aaatgatttg attatctcta
     9721 tgtaccgata aattcgtttt caaatagtta tctattcata tgagttttat ttcatgttat
     9781 ttattgttga caagagcaac tcttgttgat aaagtatcag tgtcaaaacg taacgattac
     9841 gatttgaggt gtctaaatca aaataaaagg agtataaagg tatatggcaa aaaagtcgaa
     9901 aattgctaaa gcaaagaaac aaatggcgat gattgaaaag tatgcagata aacgtcagga
     9961 attaaaagcc gcaggagatc gcaccgctct cgctaaattg ccaagagatt caaatccgaa
    10021 tcgtctgagg cttcgtgatc agacggatgg tcgtccaaga gggtatatgc gaaagttcgg
    10081 tatgtcccgt attaagttta gagaacttgc ccatcaaggg ttgattcctg gagtaaaaaa
    10141 agcaagttgg taaaaggagg aaatatcatg gcagtaccag caagaaaaac atctaaggcg
    10201 aagaaacggt taagaagaac tcatcaaact gtagtaaaac cagaaatttc atttgacgaa
    10261 gcgaatggtg attatagacg tagccatcat gtatctttaa aagggtacta taatggaaag
    10321 aaagtaatta aaggagcttc aaaatagttt aatatataac ttttaggagg gaatcaaaat
    10381 ggaaattatt tacccacctt tagttgaaga aggattaaag tattatcttg aaacgaccca
    10441 gcaatcgcta gataaatgca ctttttatcg ttcgatggta gagagaggaa ttattactga
    10501 aacgggttta ccgacccaac aggcaattga aaatggatta gtcaaagatt actatgagga
    10561 ccaaggctta tcatttgatg attttttaag aatctatcca atttttgaag agtatgacga
    10621 ggagttattt caatgtatcg atggttattg ggagatacct gttgatatga aagaaaattt
    10681 agtttcacaa ttggaatctg gcgagttaac ttttgaagat gcacaacaaa ttcaagcata
    10741 tcttgaagat cgataagtat tgaaggagga agatttatgc gtcaaaatat tattttggaa
    10801 tgtgtcgaaa ctggagaacg tctgtatctc accagcaaaa ataaacggaa taaccctgaa
    10861 cggttagaat taaaaaagta ttcgccaaaa ttgcgcagac gggcaatttt taaagaagtg
    10921 aaatagctaa gtggtaaaaa ggtgacaaaa tgggcatacc agttacaatc gtttctgggt
    10981 ttttaggggc tggaaaaaca acgctaatca atcaagcctt gcaagtctca ccctacccca
    11041 gggaagaaat tattataatt gaaaatgagt ttggtgaagt tgggattgat cacaaattat
    11101 tagttcatag cgaggaacgg gtgttacaac taaataatgg atgtatgtgt tgcagtcttc
    11161 gcagtgattt attagcaata ttgatttctt tactggaaac aatagaagaa catcaacaac
    11221 caatttcaca aatcatcatt gaaactacag gtatagcaga tccccaacca atcattcaaa
    11281 cacttttaac agcgcctcaa ctaagaggaa gagtatatat cgatagttta ttgactgtta
    11341 ttgatagtga cagttttgaa cttattgaaa gagaaccaca agcccttaag caacttgtca
    11401 tggcagatcg catttttgtg tctagaaaac aaaacttgaa tttatctgca ctaaaaaaat
    11461 tacagagaga aatcaaaagt attaaccctt tatccgatat tcgaatgttc acacatagtg
    11521 aaacgattca aaaaaaggat ttttataatt tgaaaaaatt taatcatccc ttagtttttg
    11581 aaagtgattc atcagaaaat cactataatg gcaaatacta tcatcaacac catcaagtac
    11641 atgagtttaa atcaattctc ttaacttcag atcaagacat aaaaaaagat tttttactcc
    11701 aatggattga ctggctgatt ttaaccaacc aagggagcct gtatcgtttt aaaggattga
    11761 ttgctttaca agagcaggat tttatagttg ccatgcaaga ggtaaacgaa caggttaatt
    11821 ttcaatacac cactcgacaa aaagaccaaa ccactattat tctaatcgga aaaatgttgg
    11881 atgaagaaag gatcaaacag agttttgata aattactggc ggaaagcaaa taatgataag
    11941 tagtgattgg gggtaatttc taatggagaa aacggatata tctagtgcat atcgacgact
    12001 taaaagcccg aatattaaaa ctagaaaaag ggcattaaaa attatcaaag aacataaacg
    12061 aaataaaatg aagaaattag cataattaag gaaggaaaaa gaatgaagaa aatcagtgta
    12121 ggacttattg ggatagcagc tctaggatta ttgggagctt gctccagcac taatgatgca
    12181 aaagtatcga atgataaaga tggaaaatta gaaattgtaa caacattcta tccaatgtat
    12241 gatttcacca aaaacatcat cggcgatgaa gcgaacgtgg atttaatggt tccagcggga
    12301 tcagaacctc atgactatga gccctcagct aaggatatgg caaaagctca tgatgctgat
    12361 gtttttgtat atcataacga aaacatggaa tcatgggtac caaaagctaa agaaagttgg
    12421 aagaaagctg ggcctaatgt agtagaagga acaaaagaca tgattttatt gccaggtagt
    12481 gaagaagaag atcatgatca cggagaagaa gatcaccatc atgaactaga tcctcatacg
    12541 tgggtttcac ccaaaatggc aattaaggaa gtttcaaata tcaaggatca gttagttaaa
    12601 ttgtatccga aaaaagcaaa agtatttgag acaaatgctg aaaagtattt gactaaactg
    12661 aaacggttgg atgctgacta taccactagt cttaaagaag cgaaacaaaa aagttttgtg
    12721 acgcaacacg cagcctttgg atatttagct ttggattatg gattgaaaca agttcctatt
    12781 gctggactaa gtcctgaaga agagccatct tctggtcgat tagctgaatt aaaagagtat
    12841 gttaaaaaaa ataagatcaa ttatatttac tttgaaaaga atgctaatga taaaattgca
    12901 aaaactttgg ccaacgaagc tgggataaaa ttggaagtac tgaatcctct tgaaagtctt
    12961 acaaaagaac aaatggataa tggtgaagat tatgtttcag taatggaaga caatctaaaa
    13021 gcgttagaaa aaacaactat ggttgctggt gaagaggtag ttccagaaaa agaagcgaag
    13081 gatgaaaaga cagttgctaa tgggtacttt aaagatgccg atgtgaagga tcgcgaattg
    13141 agtgattata caggcgaatg gcaatctgtt tatccgttgt taaaagatgg aattttggat
    13201 gaagttttcg attacaaagc aaaaatcaat aaagacatga cagctgccga gtacaaagat
    13261 tattatacta ctggctataa gacagatatt gatactatca acattaaaga caacaccatt
    13321 gattttgtag tcaacggtga acatcatcaa tacacataca aatatgttgg atacaaaatt
    13381 ttgaattatg aaaaaggaaa ccgtggtgtt cgttttaatt ttgaaactga tgatgcaggt
    13441 gctggtcgtt ttaaatatat tcaatttagt gatcatggaa ttgcaccaag taaggcagag
    13501 catttccata ttttctttgg tggagaaagt caagaaaaac tctataatga aatgcataat
    13561 tggcctactt tctatccagc ttcattgtct aaacatgaaa ttgctcaaga aatgatggct
    13621 cattaaaatc caatttttgg aggaaataca tggtaagaaa agtattattg tctatggatt
    13681 ccaagaatat tgatgcttat cggcgttatc gatttgcaaa gcaaatgaga ttaaatggga
    13741 gtacattagc tgatattgct gcaactttac atgttagtat tgacacagtt catagagtat
    13801 taaaacggga tcctgattgt tgttttatca aggtcaatac tggaagaagt agacgtttag
    13861 caaaacggca taatagcttg gccaattaat tgataatacc agagtagatc atttttccct
    13921 tatttggatt tgatctgctt tttattatgt cttgaattgt caaatcaagc gcaacacttt
    13981 atcaaagaag acttcataag gtgttctcca atctaaacac tttctaggtc tgttattcag
    14041 tttttcaatc atctgattta tcgttgagtt atctaccgta gctatctctt tcgattttgg
    14101 caagtattct cgaatgagtc tattcgtatt ttaatttgtt cattgttgcc aagggcatga
    14161 ggatctggaa aataaaaggg catattattc aatgcttctg taactgcgaa atgattggag
    14221 aattcttttc ctctatctgg agtgatcgtt ttgaccgcct catttggaat actgcttaaa
    14281 agttcaatca ttttctcgct cacataagcg gctttctttc tggggacctt ctttcctgct
    14341 aaaaggaagc atgattttct gtctgtcaaa gtcactaaac aagtaccacc agttttgcca
    14401 gctacaatat cggctttcca atgaccaaaa tcacttctat catctgcgga ttttggacgg
    14461 ccatggatcg aatgagtaag gcaaatcttt ccacgagact cgacatgatt ttttgtatgt
    14521 cttgtttttc ctcgatggtg caatgaacgg atcaggcctt tattgccgtg tgagaggggt
    14581 tctgtatcga aaagatcatt gcaaattgca cgttagatcg tgataaaact aattgaaaag
    14641 tccatatgtt catgcttcaa acgattcgag atttcctctg gcgaccactg aaaatcgaca
    14701 aataaatgac gaacaattga ccaaactcac ggatcattta gaatgtgata acgtccgcat
    14761 ttttttcttc gataagcata atttttttcc gctttggaag gtgaatagtt tttttcttgt
    14821 ttcttctcag ttcatgtaaa atagtaaacg ggctatgctg aatgtcgttg ccaattatac
    14881 taatagaaaa tccttcttcg tgaagcagaa agattctttc tcgttcgagt atagtaagat
    14941 gacgatactg gctcataatt attttccttc taggagtgtt ctcgacaaac ttatactagt
    15001 tggatatcga ttatgactca tttttatgtg ttgcacttaa agtgtaaatt caaggtatag
    15061 aaagaggttc cctatgaaaa aaatacctat tacaatctta actggctatc ttggttcagg
    15121 aaaaacgata cttctaaatc atattctgta cggtgatcac ggaaaggaaa ttgctgtcat
    15181 tgtaaatgac ataggggaag taaatgttga tggggtatta gtcgacaaga aaggttttaa
    15241 aagaactgga gaaaaacttg tttcgatgcc gaatggatgc atttgttgta cattacgcga
    15301 agatttgatt gttgaactag atcaattagt gcaattttct gacttgaatc aaattgttat
    15361 tgaagcttca ggaatcagca aaccaattcc aattattcaa gcaattatat tagctaaaaa
    15421 ccctttagac attgatttga cccaacgatt agagattggt gccattatta tagtagtgga
    15481 tgtaaaacgt ttttgggata atttcgaaac gggtgaaaag attcaggaaa gaaaagctga
    15541 aggtaatgta gttgaagaag aacaggatat tcgtggcttg ttaattgatc aattcgagtg
    15601 ttgtaacata ttacttttaa ataatagtga cttagtgtca accaagacga tttacaaaat
    15661 taacggtcta attgctaagt tacagtccaa tgccaaaata attaggacaa ttaaaggtga
    15721 agtagactta tcgaaaattc taaatacaaa tctttttaat atagatgaga cttcctattc
    15781 agtgggctgg ttcaaagagt tagagcttgg ctatcaaaat catattcctg aaacagaaga
    15841 atatggtatc tcaagtttta tctatcgaaa tcggttactc cttgacgcac aaaaatttgc
    15901 ccatcttacg gataaaaatt ttcctggcaa tattacaaga gcaaaagggt ttctttggtt
    15961 tgcccaacat agtgattttt cgacgatctt tgaaacatcg ggtagctata aaagtattga
    16021 atcattaaat tattgggtag ctagtttacc taaaattgag tgtcaaatga ttatacaaaa
    16081 aaatcccgaa ttgaaggaag cctggcatcc gttgtttggt gaacgcatta ataaactagt
    16141 ttttattggt atgaagatgg atcaacaatg tattaaggaa caacttgagg gttgtttaac
    16201 tgattctctt aatgcagata atttccgaaa aatttcacca tttccatgga aaaagtctaa
    16261 tatgtttagc cagatatcgt taatgaaatt tttaagaaaa gctggtgtca agataggcga
    16321 tgtcataatt attggcattt tgattattac ctcttttatt cccttatttg tattctctat
    16381 agaaaaggag aatgaaacaa ctgaagatgc tatcaaatat gccatcgttc gaattgatgg
    16441 caaggaaatg gatcgcttcg atttggataa gatcaatcat aaactcgtga attatcatcc
    16501 tgcgaaggga caatataata ttgttgagat taaagatgga agaattcgag ttaaagaaga
    16561 taatagtcct gatcaaatcg cggttagaac tggatggatc tcaaaacccg gtcaaacgag
    16621 tatttgttta ccccataagt tagttattag tattgagaaa aaagaaagca cagattacta
    16681 catttactaa tctggaaata gatgaggagg tttttaataa gaaaaagata ttattattaa
    16741 taatagcagg ggctttatta agtgtgttaa gcgcttgtgg acaagaaaaa gaagcggtga
    16801 caacaggcac aagtatcttt aacgggacct gcaatggttt agttaacagt cggttagatt
    16861 acttattttg gctcttcgtc aatgagtgtt ggtgaagaaa ttcaatagaa taatcgagat
    16921 actcacacgg ctagacagct atcttgtcta gccgtgttgt tgttctggct ttagtcagtt
    16981 gggtagtatt cttagttttc ttaaaatatt ttcctcgata taaaaagatt cgttctcggt
    17041 agttttgaaa gtttctaaag ccataggcaa ttcgttttag tactttgata tggttattca
    17101 aacattctat agggccgttg gaatagggaa cgcgcaacgc tcgtttgatt tctgtttggt
    17161 atttcttgaa ggtcgtgaac actggttggt agtgctctgg caactccttg tagtcttctt
    17221 ttaggagcgc gacgaatttt ggaacagctt gtcttctaat agcggaaaga aaatcctggt
    17281 agacttcgta gcctcgcttt aaggagtcat cataagataa tagccgatcg actatctccg
    17341 cttctgtcag atgatctcga aatgacggac gccagtagcg atgctggccc ttcaattccc
    17401 acgcattctt ttgaagtagt ctccagtatt tctttaagtg cttcgaccga cgatcccttt
    17461 ttttaaaggt cttcattact tgaattcggt gattggtaaa ggcccgatcg atgtgttggc
    17521 taatatgaaa gcgatcggcg acgattcggg cattcggaaa acgctctttc accaatgtgc
    17581 gatagggtgt atagaaatct gaaaccacta gacgtacttg ttcacggact tttctagggt
    17641 agcgcaagaa atagccgcgt aaaggattga gttgccgatt ttccacaata tcaatcagtt
    17701 gataggtctt gccgtccatc atgacaaagc tcatggctcc cgagacattt ttcactgatt
    17761 tgaattcatc gaaacacacc acttctggta acagctggtt caaagaaatg gttttaggcc
    17821 gaaggctgcg aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca
    17881 aagacataga aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa
    17941 tggagcagcg acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac
    18001 atttgaagcg ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata
    18061 agatggatac ttttttccag ccctatttaa tgatcgtttg ttcattttga tagtggcgat
    18121 gaaagcattc ctccggttga taggtcaatg tgccatcaaa agcaaaacat gtttcgtcat
    18181 tgatgcgctc tttgcgaaag gcatcttcat gaaaggtaag atttaggtct agaatatcta
    18241 gtaactcttt ggtaaactct gaataggaca tcttgaaaca ctactttgaa tggtttttgt
    18301 cgactttatt ctaaaggatt ttaaggtgtc tgtttgcgtt tattagaaaa aagaaaaagt
    18361 attggcaaca gaaggcgtca ccaacactag atattataga tcccttattt tcttcgcttc
    18421 aaattgtcaa ggagtcatca taaattttgt atggattctt ttgtttgggt agtagtcttc
    18481 taaataattt tggattaaca ccttcatctc tattttactt ttatatttgt tggagtcgat
    18541 taattctcat ttgattgatc gatgaaacgc ttcaattact gcattgtcaa caggagtccc
    18601 agtttggctc actgaatgtg tgatgtggtg gtcgttcaac agctcttgat aaataaaaga
    18661 ccgatgctgg ctacctctat cggaatgaat taccgtaggc aattctcctt aataaatttg
    18721 taatggacgg acgacaatct cttttttcat gtgtgaatct accatatagt aacgtagaat
    18781 acgagttgct aagtcaatat aggtggacaa atagaggcga ccgtcattac atggaatgta
    18841 agtaatatcc atcacccaaa cagcatcaat ttggttctgt tcaaattatc gattcaacat
    18901 ttttttacca tttctttgat tgttttttct ctaccatgag aagatttgcg tcgatgaaat
    18961 cctcttgtgc ataaattcat ttcacgcata agaacagcta caacacgttt attgatttcg
    19021 attccttcat cgtaaagttt ttgagtgatc cctggagatc cgtatcgatc tttgtgttcg
    19081 tagaacactc gtcttcctct tttagtcgtt tattttcctg ttcaagaatg tagaaatctg
    19141 ctttatcagg taatcttttt ccttgtccag caaaagcatt tttcccgtat cgacgatatt
    19201 ctgaaaccca tcgggaaatt gctagacgag agatattgta ttcttcagca attttcttgg
    19261 tagttacttt tttctcaagg atatcctgtg aaatctttag tttaaattta cgatcatatg
    19321 tttttctcaa tagagttcat tccttccggt aacgtaactg atagtcaaga aaagtgtatc
    19381 atagcccaac ttagtatcag ggtttgtcaa ctaaactgtg gaagttgata gataagagtt
    19441 tttaacaacc aaaattctct tgactatttt tgaaatcaga caaattttga ttggacacat
    19501 agttgaataa atctaccgtc gttacggaag gtaaatcgca tgcttttcaa ttctagagga
    19561 gaagtcctcg ttgaagtacc atagcccagt tctggattcg acctcctgcc cacttcatac
    19621 gtaaataaag taattttagg agtgagactg tgacaaaagt tatttttaag ctaaaaacga
    19681 attaaaaaac gttgataatt aaaatcattc gttttagatt ctatgaatca ttatagaacg
    19741 ctgtaaaatc gatactttat aattataaag aagatagttt caaatttatt gttcgaaatt
    19801 tgaaactatc tttttactta tgtcacagac tcccttagtt gagttattct ttaatttcga
    19861 atttcgagat agctagatat attgcaagca tacattcttg ataacaatct tgatcatatt
    19921 ttccagttct ccaggattgc ttatgaatca aagagctaaa tcttttgaga attgcctcaa
    19981 cagcttcttc atttccagcc tttgctaaaa tgaaatcttc ttttaaagaa ttcataaatc
    20041 taccttcttc ccatggactt ataatctatt tctttgggaa ggaggagaac gagcaaagaa
    20101 gcatacaaaa ttagttatta tgcagatatt aaaccagcat tttttgaatt tttttgtaaa
    20161 gtcgatgttt taaatttgtc gcaccctgtc ttgtaatccc cagaaccttt ccaatttctt
    20221 tatcagtttt atcaaagata aatttttcga taagaaaaat ttgttcaggt tctttcaaca
    20281 aaggaaggat ttttgctaat tttatatctt caacaaggag agtcacattt aaaacattaa
    20341 tcggttgttt aaattttata gtatctttag taaacaaata tggtgggagt tgatcaagtt
    20401 gttcatgttt ttgaatttct tcttgttttt tataataagt attagcggtg ttcaaaatga
    20461 ccttttcaaa gaatcgaatc aactgggcat ttacgcgttc gtcgttatct gacatattgc
    20521 acacctcctc cattaagatt taaagggaaa agaggcaaaa aacacgaaaa gaagctaatg
    20581 caattatgct gcgctgcgaa taacttctat aagctcacat gcattgatag gactgctgat
    20641 gtttattttc aaaaatgttt ttttagttga taatggatag ttatctttat acttagaaaa
    20701 gaaaattcat atatcttctt aaatatcaaa catgtcctta tggctaagaa aatgtcttga
    20761 gctttttacg tataataaat cttttctaaa acctcctttt ggataatatt cttattcgtg
    20821 tatagattgt attttttatt tcttccccct tcataattaa caactagaaa agagttcgaa
    20881 tagacacgta attgaacgaa aatttttatt ggattcccaa attggaatat ttagaaacga
    20941 aaaattccta ttatctgtat agtgcttaga taataacgga tcataataat tattgtcttt
    21001 gaactagata tatgtaaata gtattcatca tgatgaaaaa tgatgagttc tcggtgataa
    21061 aatgtacttt ttatggtgga atatacatga aagcgataag tggatatttt ataatgaagt
    21121 aagttagctt attttgacag aggaatagta ttttagataa cgtataaaat actatttaat
    21181 gatgtagaaa ggaaagagag aaacaaaagt gttttcagat aaagaagtct tattaaagtc
    21241 tcttcatgtt tgtttaaagt tacctatcgt ggtttttaat tccgaattca atatgataaa
    21301 agagtatcgc tcagatagaa ctacttattt tttttatgat tataaaaagt tactaactga
    21361 taatattaaa aaaactaaag atttccattt ctttactggt gattttaatg agatgtttct
    21421 tctatacatg tttaaaacca gatattactt atttggtcca tttagagcaa ataatattga
    21481 taaagatttt tttaaactaa aaatgaacaa tttgaatgtt gcaatggctg atagagaacg
    21541 actctataat tcattgcaaa atttgacttt gtattcttta ggagacattc gagatattct
    21601 gattttagtt cattattttt ttactggaaa aatagaagat ctattccata aaccgttaat
    21661 tgaatataca gaaaatctaa gtaaaattat cgagcaaatc aaaatagata atctgttatc
    21721 acaaaattac gatccagaaa tatatctgtt tctttatgaa aataaaatac tggaatatgt
    21781 taaaaatgga gatattcgaa atttagaaaa catggttttt aatctgagta atggaataat
    21841 tccaagtgtt agtggagata cgattcggtc tgaaaaaaat tattctatca ttgtttttga
    21901 aaagttagca caaacgtcta taacattagg aatggatata atagaagcgt atcaaagtag
    21961 agatgctttg atacaagaaa acgaattagc tgtatcgtta cctgaggtct taaaagtgag
    22021 ggattctggg attgtttatt atactaagga aatcggaaaa actaagatag agcatctttc
    22081 tcccttaatt tcttctgtcg ttcaattcat cggcttaaat atctataaaa gaattactgt
    22141 aaaggaaata gctaattatt tttctgtaag tgaaactaaa ttaagagaaa gctttaagaa
    22201 tgaaatgcat attacgattt ataattattg tcaatggcgg aataaaaatg ctgttttatg
    22261 gcggtttaaa aatgttgttt ttcggcggta ataaatgcct gttattagcc gatcgtttct
    22321 ttatctaagt ggtaattttt caatcgataa gaattaccag taattttaaa aactttagag
    22381 tgatggatta atcgatctaa aattgctgct gaagctgtag tattatcaaa atttttcgcc
    22441 cacatagaaa atggaatgtt tgttgtaata attgtagaag cgcgttcata tcgacgtgcc
    22501 atcagttgat aaaaccaatc agcttgtgtc ttactaatcg gcgtataacc aatttcatct
    22561 atgatcagta aaggaacatt ggtaaatatt tttagtgttc taactaatgt tcccctctga
    22621 ctcgcacgag tcaatttttc tataagatcg ttaaaaagaa taaaccttgt tttgactcct
    22681 tgaatgcatg cttcaatccc tatagctgta gccaagtgag tttttccaac cccactattg
    22741 ccaagaaaac aaatattcgt actactttct aggaagtgaa aacttttgag ttgcagaatg
    22801 tcatgcttat taatgctagg ctgaaattca aaatcaaatt cttccaatga tttaatagtt
    22861 gggaagcgcg caagtttaat tgcacgctta tctatcattt ctttttgata atccagttgc
    22921 ttttgcgtaa gattatacaa tgcatctgtc aaagaaattg tctgattcct cacggaatca
    22981 aggtaatgag gataatactc tttcattttt aataagccta gctcatctaa attggttaac
    23041 actttttggt gatttgttgt cattattatt tcagctcctt aaagttcatc atattgagtc
    23101 aatgttcgtt gtatatactc ataaatttct tcatcatctt tgtgtttcat cacgtctgat
    23161 tttaatattt caaacatatc agtttcttta tagttcaggc gcttaccagt taattcgtga
    23221 gattgtacaa cctctccttg gtagaatatt tcgataaatt tttcggttgg atcaatttct
    23281 atttcaactt cacaaccaat atattttgta gggacagaat attttgattt tctaaaattg
    23341 accattgatt cgttggtgac aatacgaaca atattgtctt caaaataggg agttaacaac
    23401 gcttcattaa ctggatgtag ttttcctttt tcttcacgtt cccatagatc aacagggcgc
    23461 tgataagtgg attgagaaat ttcttcataa ttcaaatcgt aacagagcgt attcactaaa
    23521 ctaattaagt caacggcatc atagaattca taatcataag gcttcagtct ctcaactagc
    23581 ttagctaaag attctacgga gcctttagtt tgaggtcgat aactccggca cgcaatagct
    23641 tcaaaaccag catctttact gaactcatag aaacgagtgt taaaaaccac tttacgatat
    23701 tgtgtacgtg cccgatcaac cacagttttc atattgtcga accatatttc ttttgggaca
    23761 cctccggtgt gattaaatgc ttcatacaag catgaaaata aggtgtcctg ctttcgatcc
    23821 caagttaatg taatgaattt caatttagaa aaatgtagaa cgtacagaaa gagattgaac
    23881 gtgtaagatt taccaaattt atcgtgtaag gtcatatctt ctttccaatc gacttgagca
    23941 gctgttccag gaactgtttc aacacgaagt gtcgcctttt taatttctgt gtgtttttta
    24001 ttgcgacaat aatccttaac aatagtatag ccaccctgat aaccaatttt tttcaaaaac
    24061 ttgaaaatag ccatagcgga acaatttaat tctaatttct gatcaattat ttccttgaaa
    24121 ggatctaact tggagggacg cttgggctta tctttgtctt tctttacaga gttttctgag
    24181 ccttccagat aatacttttt gacagtacgt gggtcgcaat tatactggcg tcccaattca
    24241 ttgaaattag gtttaatatc gttaatcata aaaaatcgaa ctccttcgta tacatctttt
    24301 ctcaatcaat taacctccat tcagattgag ttaagaaaag tgtatagaaa aaaatgtagg
    24361 aaaaacgaca tttttattcc gccgttttcc tacattttat agccgccatt tacattatat
    24421 ctctaagcga aaaatttcta cggctaaaat aatgttgaaa tcaaaccata ctatcagtga
    24481 agtttcttta ggtttaggat tttcagattc ttcacacttt tcgagagtat tcaaaaagta
    24541 cgcaggtgta tctcctaagc aatatcaatt aggtttagtg gacaatttca acaacattag
    24601 ctgagaggtt ttattttatt atgagactat atcatttacg tagtttggag gagttgagga
    24661 aattggattt taacaaaaaa tttttttggg aaatttctga tttgagttgt ttcgtagaat
    24721 ctgagcgtct aagtaatggt aataatagtg tgttaaatga atatgacaaa gtagtaatta
    24781 ataaagtaaa aagtttatgt gaagaagcta gagaatctat cagtcatatt aatggtagca
    24841 attttaagca aacattattt aatctgatta agctagatgc taaaatatca agttatttat
    24901 tctttttaat acatgatgaa tttatggatt atcaaaaatt ggatcagtta attgaaagag
    24961 aaagctggga agattattac gtgggtctaa ctttttcaga aaagttaaac aactacaagt
    25021 taatagatta taagtatttg agtgaaagca attaattatt catgtagagt actagacact
    25081 ttaaatatgg acaattcaat tataaagaaa aagaatcaat aatttaatga ggctgtgaca
    25141 aatgtcattt tttgtttcgc taaaaacgag tgaaaaaatg ttgataactc caatcattcg
    25201 tttaagattc taggaatcat tcagaaatgc tgtgaaaccg atactttata attataaaga
    25261 aggtgatttc aaattccgaa gaaggaattt gaagtcacct ttttacttat gtcacagtct
    25321 cattcagtaa tgcgggataa ggctccttat tccgctattt gaatactctg aagtttaatc
    25381 taagaaagag taatattttg aaaacatgct tcatcgcata gaaatgaact taatttcaga
    25441 aagaatacat acatatcttt ttatatgttt tcagctgctg aatggttaaa aagcacaaat
    25501 atccgaaaaa aacatgaaga aatgtacttt ttttgttgta atgtgcatga aattctttaa
    25561 gtaaattagt taacataagt ttaataagat tcctattctg tgaataatac gtatcagatt
    25621 tatcttttat tcttactagg aaaataacta ttaagggagt tgatttgaat aatgattagc
    25681 aagaataata agagagtatt tctagaaaaa acaaagaaac gagtattgaa gtacagtatc
    25741 aaaaaattaa gtgttggggt tgcatctgtt ttggttggtg ttggtcttgt cttgggaaca
    25801 actgaactag ttaaagcaca agatgaaatt tctccaagca ccccacttga aacagcgata
    25861 agctccgtac aagtaggtga caaagtagct tcggggaata cttttcagga gaatcctggt
    25921 tatacgaaaa attataactt ttcggattta caatttagtc ctcaagaact aactggagac
    25981 acactaaaag gcaatacaat tggatttgaa gtttacggaa aacataatat tgctgcttct
    26041 accgataatt gggaaatccg cctccaattg gatgagcgat tagccaagta cgttgaaaac
    26101 attcaagtag attctaaaaa ggttgcgggg attagttaca gaagaacttt tgtccgaatc
    26161 aatgattcat taggtagacc cacgaacatt tggaaagtta attatattcg tgcaagcgat
    26221 ggattatttg ctggggcaga aacaactgat acacaaactg ctcctaacgg ggtaattaca
    26281 tttgaaaaga atctagatga gatttttcaa gaaattggtg aagataacct taagagtgat
    26341 cgtttgatgt atcgaatcta tctggtaagt catcaagaca atgataaaat tgtacctggt
    26401 atagaaagta ctggttactt tataacagat caagataatt tctataataa gttagacgta
    26461 tctgaaaata atcctgatca atttaaacac ggatcggtaa gtaccaaata cgagaaacca
    26521 aatgttcaaa cgaaagatgg atcggggtct actggtgcta atggcgctat aatcttggat
    26581 cataagttaa caaaagagaa aaatttctct tactcggttt ctgcaacggg aacgccttgg
    26641 tatgctaact acaaaattga tgaacgttta gttccgtatg tagcgggtat acaaatgcat
    26701 atggttcagg ctgatcgagt agtatatgac gtttcatttg aatcaggaac aaaagtagct
    26761 gatttagcaa tcgagcgacg ggaaggtcat gaaaattatg gtgtaggttc aataactaat
    26821 aatgatttga ctaaacttat cgatttttct aatgcaagtc cacgtccagt tgttattcgt
    26881 tatgttcttc aattgactga accattagat gaaattttag cagaaatgaa aacagctgca
    26941 caagttgaag gaaatgaacc gtttggtgaa gacttcatct ttgattcttg gttgtcggat
    27001 acgaataaaa aattaatcca gaacacttat ggaacaggtt attattattt acaagatatt
    27061 gatggtgatg gaaaccctga cgataaagaa gagagtggag acacgaatcc atatatcggg
    27121 aaacctgaat tagaagaagt atatgatgtt gatacaacag ttaaggggaa agtattcatc
    27181 cacgagttag cgggaacagg tcacaaagct caacttgttg ataaagaagg tactgtatta
    27241 gcagaaaaaa ctattgctcc aaatgaaaaa gatggagctc caatttcgga tactgtagaa
    27301 tttgaattta cgggtgtgga ttcaaataaa ttaattgcga aagatgaatt aaaagttcaa
    27361 atcgtttctc caggttttga taaaccagaa gaaggttcaa ccgttatcaa ggaatcacca
    27421 aaagcggttg ataaacaaac cgtggtagtt ggatttaaac cagatgctaa agaatcaatt
    27481 cggaataata aaaacttacc tgaagatgca gagtattcat ggaaaacaga gcctgatact
    27541 tctaacgttg ctgatagtac gaaaggtatt gtaactgtta aaatcggaaa tcgaactttt
    27601 gacgtagatg tagagtttgc tgtaaaagct tctcaagcta tggaaaatga tgcaacatac
    27661 gtacctataa caacaacccc agaaacgaca attcaaagtg gtaaacctac atttgataaa
    27721 ccagatgttc ctctagctaa cgatgccttt tcaattttag atgtttataa taaggacttc
    27781 ggcaatgcaa gtgttgacgc aaatactggt attgttacat tcactccagc caaaggggta
    27841 ggagaaacgg aacctattac tggaacaatt cctatcaaaa ttgtttacca agatggttct
    27901 gtaggtacga ccgatttggc agtaactgta agtaaagata ttt
//
LOCUS       DAFEHS010000029        27290 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFEHS010000029
VERSION     DAFEHS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.029"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(87..455)
                     /gene="rplL"
                     /locus_tag="LP327_001377"
     CDS             complement(87..455)
                     /gene="rplL"
                     /locus_tag="LP327_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBL1725500.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(508..1011)
                     /locus_tag="LP327_001378"
     CDS             complement(508..1011)
                     /locus_tag="LP327_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBL1725501.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(1215..1342)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1421..2110)
                     /gene="rplA"
                     /locus_tag="LP327_001379"
     CDS             complement(1421..2110)
                     /gene="rplA"
                     /locus_tag="LP327_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBL1725502.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(2214..2636)
                     /gene="rplK"
                     /locus_tag="LP327_001380"
     CDS             complement(2214..2636)
                     /gene="rplK"
                     /locus_tag="LP327_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBL1725503.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            2979..3647
                     /gene="sdaAB"
                     /locus_tag="LP327_001381"
     CDS             2979..3647
                     /gene="sdaAB"
                     /locus_tag="LP327_001381"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBL1725504.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            3660..4532
                     /gene="sdaAA"
                     /locus_tag="LP327_001382"
     CDS             3660..4532
                     /gene="sdaAA"
                     /locus_tag="LP327_001382"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBL1725505.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(4623..5642)
                     /locus_tag="LP327_001383"
     CDS             complement(4623..5642)
                     /locus_tag="LP327_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL1725506.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(5815..6720)
                     /locus_tag="LP327_001384"
     CDS             complement(5815..6720)
                     /locus_tag="LP327_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL1725507.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(6779..7324)
                     /gene="nusG"
                     /locus_tag="LP327_001385"
     CDS             complement(6779..7324)
                     /gene="nusG"
                     /locus_tag="LP327_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBL1725508.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(7425..7595)
                     /gene="secE"
                     /locus_tag="LP327_001386"
     CDS             complement(7425..7595)
                     /gene="secE"
                     /locus_tag="LP327_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBL1725509.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(7619..7771)
                     /gene="rpmG"
                     /locus_tag="LP327_001387"
     CDS             complement(7619..7771)
                     /gene="rpmG"
                     /locus_tag="LP327_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL1725510.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            7933..8577
                     /locus_tag="LP327_001388"
     CDS             7933..8577
                     /locus_tag="LP327_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL1725511.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(8633..9136)
                     /locus_tag="LP327_001389"
     CDS             complement(8633..9136)
                     /locus_tag="LP327_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL1725512.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(9149..9195)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            9733..10698
                     /locus_tag="LP327_001390"
     CDS             9733..10698
                     /locus_tag="LP327_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL1725513.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(10797..11969)
                     /locus_tag="LP327_001391"
     CDS             complement(10797..11969)
                     /locus_tag="LP327_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725514.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(11956..13101)
                     /locus_tag="LP327_001392"
     CDS             complement(11956..13101)
                     /locus_tag="LP327_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725515.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(13115..14047)
                     /locus_tag="LP327_001393"
     CDS             complement(13115..14047)
                     /locus_tag="LP327_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725516.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            14164..15243
                     /locus_tag="LP327_001394"
     CDS             14164..15243
                     /locus_tag="LP327_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL1725517.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            15240..15869
                     /locus_tag="LP327_001395"
     CDS             15240..15869
                     /locus_tag="LP327_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1725518.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(15979..16206)
                     /locus_tag="LP327_001396"
     CDS             complement(15979..16206)
                     /locus_tag="LP327_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL1725519.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(16309..18897)
                     /locus_tag="LP327_001397"
     CDS             complement(16309..18897)
                     /locus_tag="LP327_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBL1725520.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(18917..19576)
                     /locus_tag="LP327_001398"
     CDS             complement(18917..19576)
                     /locus_tag="LP327_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL1725521.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(19625..20128)
                     /gene="trmL"
                     /locus_tag="LP327_001399"
     CDS             complement(19625..20128)
                     /gene="trmL"
                     /locus_tag="LP327_001399"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBL1725522.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(20349..21215)
                     /locus_tag="LP327_001400"
     CDS             complement(20349..21215)
                     /locus_tag="LP327_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL1725523.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            21324..21965
                     /locus_tag="LP327_001401"
     CDS             21324..21965
                     /locus_tag="LP327_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBL1725524.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(22027..23388)
                     /gene="mgtE"
                     /locus_tag="LP327_001402"
     CDS             complement(22027..23388)
                     /gene="mgtE"
                     /locus_tag="LP327_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBL1725525.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(23406..24305)
                     /locus_tag="LP327_001403"
     CDS             complement(23406..24305)
                     /locus_tag="LP327_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL1725526.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(24307..25104)
                     /locus_tag="LP327_001404"
     CDS             complement(24307..25104)
                     /locus_tag="LP327_001404"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBL1725527.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(25082..25786)
                     /locus_tag="LP327_001405"
     CDS             complement(25082..25786)
                     /locus_tag="LP327_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBL1725528.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            25917..26492
                     /locus_tag="LP327_001406"
     CDS             25917..26492
                     /locus_tag="LP327_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBL1725529.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            26610..27287
                     /locus_tag="LP327_001407"
     CDS             26610..27287
                     /locus_tag="LP327_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL1725530.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         7960 a         5757 c         4911 g         8662 t
ORIGIN      
        1 ctatattcat taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg
       61 atctcagtgc ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca
      121 agtttagctt tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga
      181 gcgccatcaa ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct
      241 ttgataactt taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt
      301 tcttcagcag cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg
      361 ccaaattctt cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt
      421 aactcagcaa cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc
      481 ttttttattt aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac
      541 tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga
      601 tagaagtcct tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac
      661 tttaccttcg atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc
      721 catgattttc gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt
      781 gaatacttcg tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt
      841 tttgataact ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc
      901 atcaactgtt aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc
      961 gcttacttca tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt
     1021 tcacctcctg aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag
     1081 ttactttgct caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc
     1141 gctttgctcg aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc
     1201 acttcgttca tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta
     1261 ttgaatatat tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct
     1321 tcggtacaag ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga
     1381 attatttttc agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa
     1441 catggatacc aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag
     1501 ctgctgatgg tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt
     1561 tttcatcgtc aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac
     1621 ggtaagttac tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg
     1681 taccagtttt agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta
     1741 cagtagccat catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga
     1801 ttttttgaac catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg
     1861 ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata
     1921 caacagcacc acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa
     1981 cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg
     2041 caacaggata aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct
     2101 ttttagccat tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc
     2161 tatatctttc aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct
     2221 acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta
     2281 gctgcgttta ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact
     2341 ttcgcaactt tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc
     2401 aatagaacag cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca
     2461 gaaataacaa ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt
     2521 gtgaatccca tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga
     2581 gttgctttac ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga
     2641 catacctcct taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt
     2701 tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat
     2761 agcaaattta tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa
     2821 acgctaaatt tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac
     2881 acttgcgcta gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt
     2941 gtacaaagat tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa
     3001 gtgtctttga tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag
     3061 ctgctagaat cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata
     3121 tttatctata tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag
     3181 ttggcggcct gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg
     3241 catatgaagc cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa
     3301 actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga
     3361 tcggcggtgg caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg
     3421 gtactcctac cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta
     3481 gtatactttc tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag
     3541 gtgaaaatgc cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa
     3601 gactcaaaca gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa
     3661 tgtttttatc gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat
     3721 tgatgattgc agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca
     3781 tggaaaaaaa cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct
     3841 ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag
     3901 actttttaag tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag
     3961 tcaatgcaaa aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc
     4021 ctggtgtgct gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat
     4081 tcttatttac cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg
     4141 cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg
     4201 tttgtgcaaa aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa
     4261 atatgatggg actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac
     4321 gtaatgcttt aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg
     4381 aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc
     4441 cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac
     4501 ttgcggaaga gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga
     4561 gattagttat cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt
     4621 tattaatcca tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc
     4681 atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca
     4741 cctagattca acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc
     4801 agagctttga agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt
     4861 ttgttgacta cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctaat
     4921 tgacgtccac catgattgga tacccagata ccatctgccc ctgcagagat agcgattact
     4981 gcatcttctg gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt
     5041 gttttgatgt catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct
     5101 tttccttcgc cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt
     5161 aatggaaatt ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct
     5221 gccgttaaaa tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg
     5281 aatctgtcgt ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg
     5341 gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca
     5401 gctactcctt tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat
     5461 ggcgcttgga taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct
     5521 gagtcgattc cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt
     5581 tctttcatcg tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc
     5641 atgcgctctt ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct
     5701 tcattacttg tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa
     5761 tcatactaaa cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc
     5821 tttccaaata aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg
     5881 agaggcattt tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa
     5941 cgcatagacc gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc
     6001 tagttgacca gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg
     6061 gtaatcttga tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga
     6121 ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac
     6181 ttgtggcaat gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc
     6241 catggaatgc cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag
     6301 acaagttttg atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac
     6361 ttggaccatc ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc
     6421 tgttcctact gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa
     6481 catcccttta aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt
     6541 ggttttttct atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt
     6601 atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc
     6661 tgcagttgca cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat
     6721 atagaaaaaa gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt
     6781 aatcgatttt atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat
     6841 cgatgttgac tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc
     6901 cagcaaatgc gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt
     6961 gtcgcaggct cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg
     7021 ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga
     7081 caacatacca tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt
     7141 tatgaacgat ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa
     7201 cgacacggaa aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt
     7261 ttactttgtt ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt
     7321 ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt
     7381 ctttcgcttc attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag
     7441 ccaaataagc tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa
     7501 gtttcgatga ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt
     7561 ttgatttcat cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt
     7621 atttcgtttc tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca
     7681 aacgttcgcc gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg
     7741 aacaagcgag tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga
     7801 cttatacttc ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc
     7861 tattgttttg gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt
     7921 ggaggtatag cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt
     7981 acagagacta gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt
     8041 gtgccgatct tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc
     8101 tatcgtcata aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac
     8161 gctacaaagt tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag
     8221 cttccttata ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt
     8281 acgttgctta atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca
     8341 attgcttcca caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac
     8401 agcagcattg ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca
     8461 ttgatcacgc taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta
     8521 gagaaaaaga attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca
     8581 aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac
     8641 gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga
     8701 cgataaactc acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg
     8761 gaactaacat ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta
     8821 aacgcatctt tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca
     8881 atgtttggcc gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga
     8941 cgatgacccc gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca
     9001 aactttctga aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta
     9061 acgctagata aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc
     9121 ttttcgtttt ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg
     9181 atgaaccgcg gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc
     9241 cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg
     9301 tgggatatgg tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag
     9361 taggatctgc aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat
     9421 ttgagcaacc tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc
     9481 cgaaccttgg aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt
     9541 gtttttcggt cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt
     9601 aattttgtaa cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc
     9661 ttgctaatta ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag
     9721 caggaggaaa tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga
     9781 aatggctata aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat
     9841 acacttcatt atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa
     9901 gaaacattga ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg
     9961 atcatcgtag gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa
    10021 gtagaattcc cagttgctta tattccagca ggttctggaa atgattttgc tcgaggggca
    10081 ggccttccaa aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa
    10141 aaagtccaca taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat
    10201 aatttcggta taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa
    10261 aagcgattga acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct
    10321 ttattcactc aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt
    10381 tcaaacgcat ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt
    10441 gttccaactg ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat
    10501 ttctttaaaa tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa
    10561 tatttccatc actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt
    10621 caagaagatg gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa
    10681 cagttgtttt ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta
    10741 ttcggaaaaa aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa
    10801 ttctttgccc aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc
    10861 attgaaaaca ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca
    10921 aatgtttgat tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat
    10981 gttggtgtga atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac
    11041 accccaccga taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct
    11101 gagtttttta ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag
    11161 aaagtattta aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga
    11221 accattagaa gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt
    11281 actttgcgat tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct
    11341 ttgcgattaa acgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca
    11401 taagacaaga aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc
    11461 gcacttagct caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca
    11521 tcgattgttt tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc
    11581 tgtgtaacaa ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcgccatc
    11641 tttcctactt cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga
    11701 ttaaaataaa gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt
    11761 tccttgtcat tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc
    11821 aaggcgattt tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag
    11881 aatgtggcaa caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc
    11941 aatgtccaaa tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc
    12001 taacgcaaaa aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa
    12061 atcagtgtag aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg
    12121 accaacaatc ggaatatgaa gatcaatcca atacttcact tgttccgacc ccatcatacc
    12181 agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt
    12241 catttttggt atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag
    12301 agcggtaaca atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg
    12361 aaagacagct aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt
    12421 agcgattgaa actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga
    12481 ttgatttgct tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc
    12541 cattccaatc agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac
    12601 ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc
    12661 cacttctgcc actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt
    12721 tttcatcaac gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc
    12781 aatgagataa aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga
    12841 tagttcctgt acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt
    12901 aaaattttcc gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc
    12961 aagattgcca ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat
    13021 tggaaaggcc aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata
    13081 tttataaaaa tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa
    13141 tctctaaaaa gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct
    13201 tttcttcttg taagattgta agaatcggca acaatactgt atttagttca gtagttgtta
    13261 cttggagctg acctttttta taagcgattc catcagaagc tattgttcgc tgaatcgctg
    13321 acaacacatt ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca
    13381 tatcttttag agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga
    13441 tctggtcgca aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc
    13501 cttgtcgatt cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag
    13561 ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt
    13621 tcaaccgacg tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta
    13681 atcccactaa ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat
    13741 ataaaccgca aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt
    13801 cttgaggaac gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc
    13861 cgaacaattt aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag
    13921 tcgatttccc actgccgttt ggacctaaca gacctaagac ttctccttct tgtacttgca
    13981 gatcaaaatg attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga
    14041 taatcatcat gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag
    14101 aaaagaaact agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct
    14161 gctatgcata aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt
    14221 ttgctggtaa gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta
    14281 ttcactggtg tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta
    14341 tttcttttat gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg
    14401 atcgcccctg aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg
    14461 acggttggta atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc
    14521 ttttacttac gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc
    14581 aaagatactc attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg
    14641 tcacaagaaa cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac
    14701 atccatgaca atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa
    14761 atgatcaatc aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac
    14821 actgggatgg atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt
    14881 caacactcct tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac
    14941 ttgtttgaag gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc
    15001 ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg
    15061 ccggcctttt atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct
    15121 cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat
    15181 attttccaag aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa
    15241 tgataacagt agtcattatt gatgatgatc catttgtgac gaaatcgtta caaaccattt
    15301 tagaaagcac gaaagagatc cgtgtgttag gcattggtca ttgtgccaag gatgcgctag
    15361 tcttatacga aacccatcgt cccgatgttt tgctgacgga catccgcatg cctaaacaaa
    15421 cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc cttttgcttt
    15481 tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt gtcaaagggt
    15541 atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct tataatggac
    15601 aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag aataaaccga
    15661 cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc atcaaagaag
    15721 tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt gacggcactg
    15781 ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc acgcaattgg
    15841 ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa cctgaatgat
    15901 gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc gcaaaccatt
    15961 tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg gattgttcgg
    16021 cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg attcttcacg
    16081 atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca tctcatctaa
    16141 ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt cggtttcttt
    16201 agccataaag taacgccccc cttttgattt cagcttagca tatcacactg aaaaaacaaa
    16261 agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc aagcattttt
    16321 tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat ctactaactg
    16381 gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt ctacctcttc
    16441 tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt ccattacttg
    16501 gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt ttacaacccg
    16561 tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag aatcttcata
    16621 agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga tccttgattt
    16681 caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg cttgctcttc
    16741 ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca aattacgctg
    16801 aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt cactgccttg
    16861 tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt ttcttttata
    16921 agtgacctca tttgcatcaa actggataac taattcatcc actttatctt cagaatcttt
    16981 tgccaagatg atacccgtga tttctcccat atctccattg aatacattca attcgggagt
    17041 attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc aatgtacttc
    17101 cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta acgcatcgat
    17161 ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac ggaatccctt
    17221 atttttcgct ttttccacaa ttttagagat caagggttca atttgataag catcacttcg
    17281 gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc cttctttgat
    17341 ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct gattgagttc
    17401 cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag gaccgacaga
    17461 gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa tcgctttgaa
    17521 taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca aaagtccgcc
    17581 ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc ccagaagacg
    17641 atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg ctcggccagt
    17701 tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat ccaattccag
    17761 tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt tccccgtacc
    17821 tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag cttgttcttg
    17881 cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct tctgcacttc
    17941 tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg aattaccgat
    18001 cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt gttgtatctt
    18061 tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga tttcaattgg
    18121 gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg gtacgtatgt
    18181 atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc gaattcttct
    18241 cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt tgaacccgat
    18301 tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca aagactcgtt
    18361 cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac gattcagtcc
    18421 catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca tttctctttt
    18481 tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga ttttttcgat
    18541 agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga ttcctgggaa
    18601 tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt cttgctcata
    18661 tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc cataaaaacg
    18721 ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct ctttttcacg
    18781 gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat tactaggatt
    18841 ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg gctgcatcgg
    18901 aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct aaaaaatctg
    18961 tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca gtctctaaaa
    19021 tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc tcttttcctg
    19081 tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa agtgggccat
    19141 ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt gccgattcaa
    19201 tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc aaattcggca
    19261 gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa gtacccagcc
    19321 ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc tctttgttga
    19381 taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct ttaaaaggaa
    19441 catattttac ctttttccca aatgctttga tttcttcgta actttgtggc aaaagcggag
    19501 aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc ccatgtcggg
    19561 taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact ctataataaa
    19621 ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta gggaatcctt
    19681 gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc aatgagcgga
    19741 catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc ataaatgttt
    19801 ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca tcagcaaaat
    19861 tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta cgattaccta
    19921 aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca ttccaataat
    19981 ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc tcgattaaat
    20041 gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt gcagggatta
    20101 atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt atgttcgttt
    20161 ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg tgtcagtcac
    20221 tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca ggaacctgat
    20281 aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt ttttgcaacc
    20341 tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa taattctatg
    20401 tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg ctcttctacc
    20461 tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg tatctcaaat
    20521 tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt ggctactaca
    20581 cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg acgtagttcc
    20641 tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga gagtacacgt
    20701 tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa tgtttgatca
    20761 gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga ttgttctgta
    20821 gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat ctttttcaat
    20881 tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag cgagacttct
    20941 tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga ttggatcatt
    21001 ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg ggataaaaaa
    21061 taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat caagccatta
    21121 tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg attttctgcc
    21181 aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt ctgttttatc
    21241 ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc tagaagaaag
    21301 tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac tacacatcta
    21361 ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac ttagcggtcg
    21421 gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct ggggaaaaag
    21481 atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat aacgatatcg
    21541 aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg gatggcgtag
    21601 tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa ctattgatcg
    21661 aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt cctgaagatc
    21721 ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc ttgactcatg
    21781 gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa ttgattgcct
    21841 atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag aatgcagcac
    21901 atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg gatcttcagt
    21961 tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc aaatgttttt
    22021 gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa tcagtacact
    22081 cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat caaatcctag
    22141 acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga ttgcaaatag
    22201 catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac ctacgattag
    22261 aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt cagccaaaac
    22321 aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa cagccacagc
    22381 tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga agactgctaa
    22441 aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg tcgacatacc
    22501 taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta ctggattttc
    22561 attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag cttcatcatc
    22621 aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat cataatcggt
    22681 aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt cttggtcgtc
    22741 gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg cgtcgtcatc
    22801 attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt gattaacgac
    22861 atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt acatcgcaga
    22921 gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg cgccagccgt
    22981 ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg acatcaaact
    23041 taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag cattatctgt
    23101 atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta ggtattcttt
    23161 catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt taggcgacag
    23221 ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt gcccctgctc
    23281 atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt cccttaaatc
    23341 ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac caatctcacc
    23401 tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa aggctgcttg
    23461 aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca atgaagggct
    23521 tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag cagggcacag
    23581 ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag ctggatatca
    23641 actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc cgaacgtcct
    23701 tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg caaatcgata
    23761 tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata ttttttcaca
    23821 aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg ctttgcaaaa
    23881 agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac ttggttttcg
    23941 taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt tggatggtat
    24001 tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg ttcatcttca
    24061 tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc ttcagcagga
    24121 atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc tacttgatca
    24181 ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt tgagatccct
    24241 ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc ccatgtaaac
    24301 tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat gcatatgtcg
    24361 ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc ggatatttgc
    24421 ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt ccaattttat
    24481 ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa acactcgatt
    24541 attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga tcacagcacc
    24601 gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca atccatctcc
    24661 tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg tacgattggc
    24721 ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt catccaaata
    24781 actgatccga acatccagca agggataact aacactttgt tcccggttcg ttcgtaagct
    24841 gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta aatgacctgt
    24901 atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga acgccgacaa
    24961 taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat ctcgttgaat
    25021 ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct ttgcttcttt
    25081 attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct ttcttcaaaa
    25141 gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac cgctgtgctt
    25201 cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca gcttctgccg
    25261 cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta taattcagcg
    25321 aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt tccgccaaaa
    25381 ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga taagaacggt
    25441 agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc atgtcttttc
    25501 ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac atgatcctta
    25561 acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc cgtaattgtg
    25621 attttgttac aatactatcg actggcttga ctctcccagt gacaaattca atcgggacat
    25681 gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc aattctgata
    25741 cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct gatacctcta
    25801 cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt actcttgatt
    25861 tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc gatcaaatga
    25921 cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac gagatattca
    25981 tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt tatttcgata
    26041 ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc gttggtcatc
    26101 aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca acggatacct
    26161 ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt tcaggcgcag
    26221 tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa accggaaagc
    26281 ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt gatgaaagct
    26341 ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca tctgtgggaa
    26401 aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct gctaagaaca
    26461 agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca aggcacttga
    26521 gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta gtcactagtt
    26581 gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt gttaatcctt
    26641 taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa gaagagtacg
    26701 ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat ttgcagacaa
    26761 ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa cgtaatcact
    26821 tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt caaggaaaga
    26881 aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat gacataccat
    26941 attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg aatatgttta
    27001 aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa aaaattgctc
    27061 gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc tgcgaccgtg
    27121 attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc acagagctgt
    27181 gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat acaaatgatt
    27241 ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DAFEHS010000030        25684 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFEHS010000030
VERSION     DAFEHS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.030"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(143..577)
                     /locus_tag="LP327_001408"
     CDS             complement(143..577)
                     /locus_tag="LP327_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725531.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(687..2546)
                     /locus_tag="LP327_001409"
     CDS             complement(687..2546)
                     /locus_tag="LP327_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL1725532.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(2664..3823)
                     /locus_tag="LP327_001410"
                     /pseudo
     CDS             complement(2664..3823)
                     /locus_tag="LP327_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(4130..5554)
                     /gene="araA"
                     /locus_tag="LP327_001411"
     CDS             complement(4130..5554)
                     /gene="araA"
                     /locus_tag="LP327_001411"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBL1725533.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(5570..6268)
                     /locus_tag="LP327_001412"
     CDS             complement(5570..6268)
                     /locus_tag="LP327_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL1725534.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(6225..7886)
                     /locus_tag="LP327_001413"
     CDS             complement(6225..7886)
                     /locus_tag="LP327_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBL1725535.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            8104..9504
                     /locus_tag="LP327_001414"
     CDS             8104..9504
                     /locus_tag="LP327_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBL1725536.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(9756..10835)
                     /locus_tag="LP327_001415"
     CDS             complement(9756..10835)
                     /locus_tag="LP327_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL1725537.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(10921..11880)
                     /locus_tag="LP327_001416"
     CDS             complement(10921..11880)
                     /locus_tag="LP327_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL1725538.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(11892..13403)
                     /locus_tag="LP327_001417"
     CDS             complement(11892..13403)
                     /locus_tag="LP327_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL1725539.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(13425..13988)
                     /locus_tag="LP327_001418"
     CDS             complement(13425..13988)
                     /locus_tag="LP327_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL1725540.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(13994..14857)
                     /locus_tag="LP327_001419"
     CDS             complement(13994..14857)
                     /locus_tag="LP327_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL1725541.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(14871..15767)
                     /locus_tag="LP327_001420"
     CDS             complement(14871..15767)
                     /locus_tag="LP327_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1725542.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(15942..17231)
                     /locus_tag="LP327_001421"
     CDS             complement(15942..17231)
                     /locus_tag="LP327_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL1725543.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(17203..18720)
                     /locus_tag="LP327_001422"
     CDS             complement(17203..18720)
                     /locus_tag="LP327_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL1725544.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            18918..19808
                     /locus_tag="LP327_001423"
     CDS             18918..19808
                     /locus_tag="LP327_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBL1725545.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(19881..20705)
                     /gene="nadE"
                     /locus_tag="LP327_001424"
     CDS             complement(19881..20705)
                     /gene="nadE"
                     /locus_tag="LP327_001424"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBL1725546.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(20717..22189)
                     /locus_tag="LP327_001425"
     CDS             complement(20717..22189)
                     /locus_tag="LP327_001425"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBL1725547.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(22826..23188)
                     /locus_tag="LP327_001426"
     CDS             complement(22826..23188)
                     /locus_tag="LP327_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL1725548.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            23411..24163
                     /locus_tag="LP327_001427"
     CDS             23411..24163
                     /locus_tag="LP327_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL1725549.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(24303..25226)
                     /locus_tag="LP327_001428"
     CDS             complement(24303..25226)
                     /locus_tag="LP327_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1725550.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(25241..25513)
                     /locus_tag="LP327_001429"
     CDS             complement(25241..25513)
                     /locus_tag="LP327_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725551.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         7502 a         5377 c         4330 g         8475 t
ORIGIN      
        1 tttactcgat tttgagtaag tacaaggttg ttgttggtta taggtatgtc attatcataa
       61 aggattttga tcactctatc ataaaggtaa tattacattt atattagata actctagttt
      121 ttattgttat caaagatttc ttttattttt tcaacaacaa attagtcatt ttttggatgg
      181 cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc agatgtattg aatagatctt
      241 catctacccc tgattcaaga tcgatacttt ggatcatttc ttcacagcct gctacatatg
      301 aaagataagc tttttcaaaa tttttatgaa tgcccattgc ttttgctggt ggacgcaaag
      361 tactgacttt cataagcatt gcccgatatt tttttgttcc ttcttcaaat aacaggatga
      421 tctcttcaag agtcgatttg tccagttcac ttgtttttcc ttcatcaatt gcgtttctga
      481 tcacttcata tttcggatgc attccttctc cgattgcttc tgtttccttg acgatttgat
      541 cgatggactg cacataatag ccaatattcg gtaacatgtt cgttttccta ctttctccat
      601 tgttgttatt atttattcta cttgttttta tcctcaaatg acaagtactt gaaaaaattt
      661 aatggtttct atacagctcg ccattcttat tcgtgctcaa agaaaccatc tgctaaacta
      721 tattgtttcc ctgcgacaat gtcatattgg atcaattgat attcgtcgta aatctcttgt
      781 tgcgagcgat tgaattgcgc ttctttatac cactgtccgt tttgatagta cagttctcgt
      841 tcaaacgccg gaagttcctg ttctaatgat aaaagcagct gataaaagcc atttgttgtc
      901 tgattcgtct gttccattag attcgctgca aaataaaacg gactggtgat tgcatcgtga
      961 gtcgtttgct tttctaattc tcctttactg tcgaacataa gaaattgtgt ttcatgcaat
     1021 gtttgtttgt cattttttgc ttggatcgta tctgaataga tccctggaag atgatccccc
     1081 caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt cttccgtgaa ctgcttcagt
     1141 gccgtgctgc tataggaaat atcttgcaga taattctcta agtcaagtgt cccacttcca
     1201 tctgaagttt ttgcaggata gtctagatta tcatactttc catcgtaggg catatgtgtc
     1261 tgcatcgtga ctaaatgaat gaactgaggt gttttttctt ccttgagaag tgtcattact
     1321 tctttgtaag ctgactcatc agaaatatat ggattattct ctatcgtatc cgtataggtc
     1381 atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct gataaacatc ctctctttta
     1441 tacatcgaag tattgtatgg atggatcgct gtcgtctggt atgattgtgc gtttaacgct
     1501 gaaacgatag aaggaagctg atccattttt ggtacaagca tcgtgtaagg ggtggtgagt
     1561 tgcgcattaa ataacgccat agaaaaacca gtcaatgctt caaattcgat atttgctgtt
     1621 ccaccgccat agttttgaga aagcatattg ccactatatg tttgatcagc tacttcataa
     1681 taatctgcca atggctcacc agatacttct acgccgttta atcgactagg atctgagaaa
     1741 ctttcactca tcacgaacac gatgttcggc gactcttcct ctacagtttt atttttttca
     1801 tcggctagct tctggtattt ttcagtgatt tctttgatct tttctttcga atatccttca
     1861 ggttcatcca ttggttctac cttcagattg tacaaaaacc caccgataaa accagtgtta
     1921 tagtagttca ttttctggct gtaaggtatc catagtgccg ttttattata tgcttttcgc
     1981 aacaaattat cgggattgtt gaaattactg atatacccta gcagtccaat tgccacaagc
     2041 agtgtcgtta atcggatcaa ttggaatcgc cttccttttt tcaggcttcg aaacatgtac
     2101 cagcaaaaca atcctatcac ggttcctgcg gcagctaaga tcactgtaaa cagcatagtg
     2161 ccggtcatat ctttcagcaa tccgatttca gtgatcatct tcaaatcgtc tggataaatc
     2221 ggttcttgcc gataactcat tttcatataa tttgcaaaac ctaatacacc gatggctacg
     2281 acatacgtaa gggaacctaa agggacagat cctgctagtg caatcagaaa aataagaata
     2341 atcagcaaga cgagacaggc taagaagaat ttttccgtat gccaagaaaa agcaaatttc
     2401 aacgcaaggt ctacggatag atcattttga caccactgca aaaataaatt actaaaaaca
     2461 acaagaaaaa agatactgat gattgctgct gttcctttta aaatcttttt ctgtttggtc
     2521 gaaagtctgc ttatatatct tttcaaatgc ttgctccttc atttctatca ataaaagaat
     2581 gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa agaacggaaa gaagaattaa
     2641 tgaaatccaa atattctctt tctttatggc ctttctggtt tctttaataa gcggaccttt
     2701 cgtactccag aaattttttc tagctgttgt ttcacttgat tgagctgttc aaaatcagta
     2761 ctgtctatgt caatcaatat gtagacatgc tcattcttat ggttacgcga tatatctgcg
     2821 atcgctagct cgttctcatt cagcagagaa aatatctctg ctaatatatt ggttttcttt
     2881 tgataaaata tagtgaatcg aaacggcgct tggaaaaaca ttcgtgcgga tggataattg
     2941 accgcttctc taaccgtacc gaacaaaaga taatctttta aggcttcaac tgccaaacgt
     3001 ccgctgtctg ccaaagcttc ctgagtcgat cccctagatg aggcaacata agtatacggt
     3061 cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga tgttgttttt
     3121 ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca ttgataagta
     3181 cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt gtatcttctg
     3241 tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct aaatctgctt
     3301 gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc atccctaagc
     3361 tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga ccgatggttt
     3421 tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt tctaaaatat
     3481 taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg gaagataaga
     3541 gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt gtattcatga
     3601 cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca acaccagctc
     3661 ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta ctacgaataa
     3721 ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt aacttttcta
     3781 tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc cctccttact
     3841 aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact tcctagaagc
     3901 aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg acaagttact
     3961 gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga taaatggtgt
     4021 tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag caaatttcgt
     4081 caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt tatctaataa
     4141 ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt gttagattta
     4201 gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca ccttcatgga
     4261 aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct tcagtcgttg
     4321 gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa tctgccatcg
     4381 atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga tcttctctat
     4441 ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc gtcggatcta
     4501 cttccatcat atgggattcc aagattgctt cttggccact agctagctcg taagtataat
     4561 cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga tcaatcgctg
     4621 cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag
     4681 ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta aaagcgttat
     4741 agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt acttttacat
     4801 gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat tccttgtact
     4861 cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt acaagatctc
     4921 cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact ttgtctcctt
     4981 ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt ccaaaacttt
     5041 cattataggc tactgcaaca tccatccaat cagcaatctc tttttggatc tcaggtcgtt
     5101 tccagtagcc aacgacgatt ttattttgtt tatttaatcg agcattgatg aacccatatt
     5161 cgcggtcacc atgagcggat tgatttaaat tcatgaaatc catatcgatc gttttccaag
     5221 gaatgctttc gttatattgc gtagctaaat gaagcaatgg tttttgtaga agctttgtcc
     5281 cgcggatcca catttttgct ggtgagaaag tatgcatcca agtgatcaca cctgctactt
     5341 cttctcgata attgacctct ttcatgattt ttgtgatcgt atcagcagta actgctaatt
     5401 cttgaaggcg gattggataa ggcaattggc cattttcatt cagttcatcg accatttctt
     5461 gtgcatgttt ctttacttct cgcagtgctt cttccccata taaatgctgt gatcctacaa
     5521 caaaccaaaa ttctttttct ccaatattca acataattag ccactccttt tatttgtttt
     5581 gtccgtaata cgcatttttt ccgtgttttc gtaaataatg tttatctaaa atccgttgag
     5641 gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg catacttcat
     5701 ctagtacgac tgcgttcatt accgcacttt gcgcatcttt cccccaagta aatggaccgt
     5761 gtccgtgtaa aagtacccct ggaattgcta agggatcaat tttgcgttct ttgaatgttt
     5821 ctatgataac ttttcctgtt tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa
     5881 atcgagcaca aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa
     5941 catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg acgattcctc
     6001 cgatttgagg aaatgaacga tataaaacag catgagtagg cgtatccgaa gagggattga
     6061 gtttaccttc aatgatatga ttgtccaagt ccacaacgac catgtcgtct gctgtcatct
     6121 cttcataact gactccgcta ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc
     6181 tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt
     6241 atacttcttc tttcagttgt tccagcattt tctatcactc ctttttcttg caattgatga
     6301 atcgctgttt tttcaatgtc gatcccctca cggtaacgtc tcataaatat ctgatagcct
     6361 gctatttctt ctgctgaagg tgtgatggtg attccttggt cttgtccaaa cacgtcttct
     6421 gccaagaagt tttctaaaga cttcccatgt cgatccatca tataggctgc taataacgcg
     6481 attccccacg ctccaccttc tccggctgtg tccatcactg taactggtgc ctccatagca
     6541 gatgctagaa tccgctgtcc aacttctggt gttttaaaaa tccccccatg ccccactaaa
     6601 cgatcaattt ggacattctc agatttcaat atatccattc cgattctcat tgcaccaaaa
     6661 gcactggcta aatggatccg catgaaattc gctaaatcaa aacggctacc tggtgtacgt
     6721 acaaataatg gccttccttc atttactcct gtaatgtttt caccggaaaa ataaccataa
     6781 gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa taccgtgaat
     6841 aatttatctc tgcttatctc catacccaaa gaattggtaa attcttcaaa aatactgacc
     6901 caagcattga tatccgaaga gcagttattc gtatgaacca tgcctactag ttctcctgaa
     6961 ggtgtagtga ccatgtcgat ttccggatgg acattttcta gttctttttc taatacgatc
     7021 atagcgaagg cggaagtacc agccgaaatg ttccctgtcc ctttggctac actgttcgta
     7081 gcgaccatac cggttcctgc atccccttct ggcggacaaa ccgggattcc tggtctcaaa
     7141 tttcccgtag ggtcgatcag ataagcccct gattcgctta acttgccagc ttcttctcct
     7201 gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt
     7261 aactgaccaa aaatattttt catttcttga tggtaggttt tcgttgaagg atcgatagga
     7321 aacatgcctg atgcatctcc tacgcctagt actttctcac cagtaagctg ccaatggaca
     7381 taacctgcaa gcgttgtcaa aaaattaatt tctttcacat gttcttcttt attcaaaata
     7441 gattgataca aatgagcgat actccatctc tgcggaatat tgtattgaaa tttttccgtt
     7501 aatattcgtt ctgcttcttc agtgattgta tttcgccaag tacgaaatgg tactagtaac
     7561 tgttccttgc tatcaaatgc tagataaccg tgcatcattg cactgaaccc aatcgacccg
     7621 attgtcgtaa gattctcgcc atagatttcg aatacttctg ctgccatttt tcggtaactg
     7681 acgcgcagtc ctttccaaat atcttctaat gaataggtcc agataccatt ttctagtttg
     7741 ttttcccact cataactacc agcggcaata ggagtaaaag atgaatcaat caatacagct
     7801 ttgatcctag ttgatccaaa ctcaatccct aaagatgtac gaccatctat gacatctaac
     7861 atactttgcg catttacttc cttcataatg atttaactcc ctccttctcg tcaaacctga
     7921 tcaaaccgtc tttttagtag cgcttacaat gattattgta tacttttgta cgtacatgtc
     7981 aagcagttat ttacaagaat acaataaaaa acacttgttt tgtacgtaca taagttataa
     8041 aatgaaattg cttacaaaat ctgatcaacc gccttctcga aattctagaa ggagagaata
     8101 cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt atttttttgg cgcatttggc
     8161 ggcattctat ttggatatga tattggtgtt atgaccggcg ccttaccttt tttacagcat
     8221 gattggggtt tggcaggtaa agcttctttg ataggatgga tcacgtcttc ggtcatgtta
     8281 ggtgcgatcc ttggcggctc tttatctggt cttctttctg ataagcttgg acgacgaaaa
     8341 atgattcttc tttctgcact tatttttatg gctggttctg tcctttctgc atctgcccct
     8401 cacaatggtt cttattttct gattgcagca aggattcttc taggtttagc tgttggcgct
     8461 gcatctgccc tagttcctgc ttatatgtct gaaatggctc ctgctagact aaggggaaga
     8521 ttgtcaggga tcaatcaagt gatgattgct tctggcatgc tcctttccta tgttgcagat
     8581 tatttgctaa aagggttgcc tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct
     8641 gtccccgcgc tgattttatt tttcggtgtt ttagctctac cagagtcacc gcgttttctt
     8701 atgcaatctg gccgcttgga agaggcaaaa agagtattga actatattcg gacaccaaaa
     8761 gaagctgaac aggaattcga acaaatccag ctgaatgtca aacaagaaaa gacaaccgga
     8821 acttcttggc atactttgtt tttagaaaaa tatcgttcct tagtcattgc cggtattggt
     8881 gtagcagtat tccagcaatt tcaaggagct aatgctattt tttactatat tccgctgatc
     8941 gtagaaaaag cgactgggca tgcagcgagc gatgcattga tgtggccgat catccaagga
     9001 atcattttag ttgctggttc tttgctcttt ttggttattg cagacaagtt caatcggcga
     9061 actttattga aaataggcgg tagcgtgatg ggactttctt tcatcctacc agctgtttta
     9121 ggaactgtac tggatgctca tacaaattcg ttgttgattc tcttgttttt atgtatttat
     9181 gtcgcatttt attcttgcac atgggctcca ctgacatggg taatcgtagg cgaaatattc
     9241 ccactagctg tccgcgggcg agcatctggc ctagcctctt cttttaattg gattggttct
     9301 ttcctagttg gcttgctttt tcctgtgatg actgcttcta tgtctcaaga aatcgttttc
     9361 ggcatttttg ggatcatctg ctttttaggg gttttattta ttcaagagat cgtaccagaa
     9421 actcgtggga aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa
     9481 cgtatttcta tccatcattc ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata
     9541 agaaggaacc gcttagtttc cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
     9601 tgatgaatat tgtgacttgt gaccagttgg taggagaaca ccgtttagtg gagaggtggt
     9661 gaaagaaacg ttcagccttg agcgatgaca aaagctctca caaacattgc gacgagtaac
     9721 cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt
     9781 tcccgttcaa ctagtacagg ttcatagaga atatttggct gtgcctgtcc tttttcaatc
     9841 gttgtgacga tccaccgtgc ggcatcttgt cccaactttt cttttggatg cgtcaatgtt
     9901 gtcaattttt tattccctaa atggcttaaa gaagaatcgt ctgttcctac gatcgatagg
     9961 tcatctggaa tatgatagcc attttctgtc aacttatcta gcattaaact agctatttga
    10021 tcgttatagc aaatgatgcc atctaaagct tgttcttctt tcaaccgttg tactgttttt
    10081 tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc
    10141 acattatact gttcacacgc gctgataaaa cctttcatcc tgtattttcc ttgaagatcg
    10201 tcgatttttg tgatgaacaa cagacgctga tgtccttttt ttatcaaata ttctgctgcc
    10261 ataaaaccga ctttcacatc atctaaacaa atataagagg catttaactc ttcataagca
    10321 gcattgatca tcaccatcgg aatgccttgt tctctcaaag caacataaag tgctaagttt
    10381 gggttatact gattactttt cgtaggctca acgatcaaac catctacccc ataagcaatc
    10441 attttcttca aacactcttt ctccaattcg tgatcgttat tcgtactggc taaaagcaat
    10501 gaatacccag attctcgtaa ttcattttcg attcctcgga taatcgacgg aaaaatataa
    10561 tcagagagat atgtggtgat gacaccgatc gtttttgttc tgttcaatgg tgttttttct
    10621 ttcttatagc tgtcatctac atatgtgccc gacccttttt ctttacgcag atagccctca
    10681 tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc
    10741 agcttgaatt ctgagggaat ggttgtattt acttcgtatt cctcacgaag gatcttttgt
    10801 tttatctctt ctgcaatatc ttgatatttt gccatttgcc ttgccccttt ttatttgtac
    10861 gtacatatat cataacgagt ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc
    10921 tcataaggca tacggaacag gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa
    10981 gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga tccccttttt ggttcttctc
    11041 tggtcgagca tgatagatca aaatatcaaa gcacccgtct tctgaaaccg taaacgagtt
    11101 gtgaccagga ccaaattgct tatttttttc agaagaaacg aatactggct ccgataattt
    11161 atgccatgat tcttttaata aaagattctt gtgctcatca gcccacagca ggcccatggc
    11221 atagttttca tctgttgcac taccagaata cgtaatgaat attttcccat tgcgagtaag
    11281 gactgctgga ccttcattta ctgaaaaacc ccgtttttcc cattcatact caggtataga
    11341 aaggaggatc tgcttcccct ttaacgtcca aggattatcc atttcggata gataaagatt
    11401 agaattccca gtaatttcag gatcttgttg ggcccacaca taatatagct ttccttgaga
    11461 agagaaagtt gttccgtcta aactgaaagt ttcccttttc gtttttattt gtcccttttc
    11521 tatccaatct gtattcattg gatctatatg ctgattttca agtacgaaca tgcggtgatg
    11581 atgagattca tcacgtatag ttttatgatt gcttgcagca aagtaaatat accattttcc
    11641 attgatataa tggacttcag gtgcccagat caattcactc atcggtcctt cttcatgagc
    11701 ctgccataca atcaggcttg cagcatgttc taattcattc agtgactttg cccttcttaa
    11761 ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa taataaccat ctgtatgttt
    11821 atatacccaa ggatctgccc gttgccgaac gataggatta ttataaagtg tcttcatcat
    11881 aacaaatttc ctcacatttt ctttattcta attacattcc aagagtattt ttctatccct
    11941 acatttaatt cgtcatgttt ttctattgaa aaatagttta tttcaggttt tattttttct
    12001 ggttcttcac gtgtattttt gtccaacaga tcgctactgg ttaatgtgta gtgctctcct
    12061 tgatctagca aattgaaatc atgttcaatc gttatctttt gttgttcatt tgtgcgattg
    12121 atcaagaaaa tagtcagctg attgccgtta tctacgatca cactgtccac ataaggaaca
    12181 ttttttatct tttcacaatc ataaacaggg caaatgattt gttgtttcaa tacttgacct
    12241 ttagcatatt tcgataagtg ttgcatcaca taataagttg gattcctcca tgcttgtcct
    12301 ttagcatctg tcagtattaa aggtattgtg tttactaaca atgattgaca actaatcttt
    12361 acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac caaataataa tgtatcttcc
    12421 atagtaaagt aacaccaatc gatgggacta ccgacttcaa aatccctatg tcttttttcc
    12481 ggttgggcat gtacattcca ttcatcaaac gctagataca ttgttttctc acttctcaaa
    12541 tatcctttta cataatcaca agtagcaacg atttcttcaa tttgacgatc gaatcgataa
    12601 gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt taggaggttt agtcaagtct
    12661 tcttcatcat acttatcaat atagttatgc aaagctaaat aatctacatt ctcataagct
    12721 tcttctaatg cgatacgatc ccattcaggg tacgatggta aacgtggcgt agagcttcca
    12781 actaagattg tttcaattga atcatccact aacttcatga cttttgaggc ttcatttgct
    12841 aacctaccat attcataagc actcttagcc cccacttgcc atggaccgtc tagctcattc
    12901 cctaaacacc ataatttaac gccaaaaggc tttttttgcc cattttttat tcttaaatca
    12961 ctccagaagg ttccttttgg aaaattacaa tattctacta aattttttgc ttcatcaatt
    13021 ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt gtacatgact gatccattta
    13081 aaaaattcgt ggagtccaaa agaatttggt tctatacctc gccatgctaa atcaattttt
    13141 tttggtcgtt gttctttcct acctacagta tccacccaat cataaccaga tgtaaagttt
    13201 cctccaggat agcgaatgac acctaattct aattccttta cataagagag gacatcttgt
    13261 ctaaacccgt caccatcagc tgtttcatgg cccggttcat aaatccctgt atagacgaca
    13321 cttcccatat gctcaacaaa tgagccaaac agccgagaac tgatctctcc taccgtaaaa
    13381 tcctctctga tttttacttt cacttcttcg tccaacagct ttccctacct ttcatttcct
    13441 atagtcaaga taatagatac aagtttccat acgccaccag caaagataaa aataaaagga
    13501 gggaaaagaa atatgagcag tacaaatcct aataacaata cgcaacaaag aatatttctc
    13561 aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct
    13621 ttgttttcag aaatcacata tatccaggat aaaagaaaca tggaataaat aagtaaaact
    13681 acatatccac taattgaaag tacgattgtc tgttcttcta ataattgatt aacaagaaaa
    13741 taaataatga tcgttaacag actaaggaac aagttcttac caaaatgttg ctttattcct
    13801 tgaaagaaaa aataaatgat atttcctgat ccctcacctt tccattgcgc cattacataa
    13861 aaaacagcac atgtagcagg cacgatagta ataacaggca aacacacgat tacccagaga
    13921 atacctaata agatgtaatc agaaaccaca atcaactgtc cataaagttt agtctcaact
    13981 attttcatct tcctcactct ttcattccag acatagctgt tccttgaaca aaatatttct
    14041 gtgcaaaaat ataaaccact agtaatggca gcattgaaat aaccgttcca gccattacag
    14101 gtccataaaa agaaatgttc tgccctgtaa ataaagctaa ccctgctggt aaagttctca
    14161 tttttgaact acttgtcaat atcaatggat aaagcaagtc attccaacta ttcatcagcg
    14221 tgaaaatccc taatgataaa agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa
    14281 tattaaactc acttaatcca tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa
    14341 taaaaaagga acgcatcatg aagataccaa atgcagttgt catacgagga atgatcaaac
    14401 ctgcataagt atcaagcatg ccaagcaaat tgacttcaat aaatagagga atcatgaaga
    14461 cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat tttcctttga
    14521 aacgcatacg agcaaatgca taccccgcaa gagaatcaaa gaatacagaa gtgatcactg
    14581 tccctccagc aaaaatcact gtatttttga tgtaatctaa taaagggatc gttgtccaaa
    14641 ccttaatata gttatctagg gtatagctat ctgcaaatat tctaggcgga aatgagataa
    14701 tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac gcaataacca
    14761 acaatataac tagcatacta atcgttcgaa tgattttttt accactaact gcttttcgac
    14821 tattcacagt aactgtaatc gagctttctg gtgccataca ggtttccctc ctatttcatt
    14881 tgtttttctt tccgtgacat gtacacatta gtggagatag ctatagttgc aacaatgata
    14941 aacaaaaccg aagacagtgc tgatgcatag cccaattcat aaggtgctgt aaatcctcga
    15001 ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca
    15061 tatacttggt caaacacttg catcgaacca atcagattcg ttaaaataca aaaacctaag
    15121 gagggaacga tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc
    15181 atttgagcag attcatacaa agagggagag attccctgaa tagcagtcaa gagaatcgtc
    15241 atagtaacac caaaattctt ccaaacagtc atgatggcta ccgtcggcat agcaagggtt
    15301 ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc
    15361 cctacattcg gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata
    15421 actggaatgt aaaatgtcga acgacaaaaa cgattgaatc tggtattttt agataatgct
    15481 actgctgcta ataaaccaac gataatttgt gttggcgttt cgagtacggt gaaatataaa
    15541 gaatgaatca acgaattaac tgctcgttca tcactaaata tacgtgtgaa attttcaaaa
    15601 cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg taaaactaat agcaaatgtt
    15661 cctaaaagcg gtatgacagt aaataataat aaaactaaaa aagatggagt taaaaacaaa
    15721 tacgcagcac gatcaggacg attccaatat ttggtgaatc cgttcatata gtccacctca
    15781 tctttcattt tgataaaata tacaatcttg gtttaaacga atctgaataa gatatccgaa
    15841 caatcgctgt gatttttgca ataacaccta tctgcaaata ctaggtcgat cattcagata
    15901 tctgtatctt gccaacagtc tatcctgtat aaagttctgt actatttttg cagtaattga
    15961 ttgatggcat catctgcatc gttcatgagt gtctcaggat cttctcctgc taataatttt
    16021 tcaatcagtg gattgatgat atcgttattg atcgcagaga tttgggtaaa acctggtaaa
    16081 tatggctctg catagtccat ttgtttcgat aattcagata caataggatc attttttact
    16141 tcttcatctt ttgctactga atccaagtag gcaggaaaac cattttcgat actccatttt
    16201 ttaccaattt cagttgtatt ccaatatttc aaaaactcat agacagcttc ttttttatta
    16261 ttatctgtac ttgccggaat ggcatatcct acgccatcta aaatagctgt ttttttgtct
    16321 gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg
    16381 ttatttaacc atggcccatt gatttcaatg gctagttgac cagcattcat taaattgtcc
    16441 atttcggcgc cagaaatcga ttctggacca gtcttgtcta catggatcat tttttggatc
    16501 gcttttaacg ttttaaggtt ctcaggagac gcaaattcag acttagtata atcatcgttc
    16561 actaatttac cgccatttgc taacatccag ttatataacg gtgcagttcc atccttattg
    16621 aagacaaaac ctgatacatt tttactacta tcagttaatt ttggtgccat ttctgtcaat
    16681 tcttcccaag tagtaggtgg ttgttcagta tctaatccag cttgtttaaa caaatcatta
    16741 ttccaataca tatacatgcc ttgtacctgc atggggacaa aatactgttt accttctact
    16801 tcacctaatt caatagccgt ttctttaaaa tcagatttat ctactccatc atattcccag
    16861 aaatcgtcta atggttgtac cgctccgttt tctacatact gtgcaaaatc tggataattt
    16921 aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg taggaagttt ttcattcaaa
    16981 ttggcccaag tcatgatatc catttcaatt ttgatgtttt tatcatttgt tttattaaat
    17041 tcattaacga tttcttgcaa tatttcacca tcagatgcag taaacccatt ccaaaaagtc
    17101 aaagtcactt ctttatcaga atcagattct gctgaacttc cgccaccgca agctcctaaa
    17161 atacctgttg cagctaccaa caaagagcca attaaccatt ttttaccttt cattttttgt
    17221 tcctccttca tctgataaac gacatctgac cacattccaa gatagcggtt tcaataaaca
    17281 attgattttc ccatcttcag cttttacatt tgttgaatga cttggtttta cttgttcact
    17341 tttaggaaga ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat caatcactcc
    17401 ctctaatgat aattcagaca agtgaacagt tagattcatt tcttcttctc cacggttaac
    17461 tgcaaatatg acaagttctt tgtcatttat tacggctata ctttctaaat acggcacttc
    17521 ttcaaaatct tccgtcgcat agtttgctga acgaatttgt gggcttaaca cctctccttg
    17581 tccatacaca gcagcttgca taaatggata atagatcgtc tgtctccaag cttctccgtt
    17641 tgtttctgtc ctgattggtg caataacatt taccaattga gcaagacaag caatctttac
    17701 tctatctgca tgtttcaata aagtgattaa tagacagcct aatagtaatg catcttcaaa
    17761 attatagtta tcttctagta aaggaggagc aatctgccaa ggcgctgctt gctcatcttg
    17821 ttccgctgaa tgataccaaa tattccattc atcaaaggac aggttgatct gcttcttact
    17881 atgtttcttc gccttgactg tatcgcacat cgctgtcact tcagaaatga accgatccat
    17941 gtctaaggaa cgtgcaagat aatgagaaag atcatttttt tggttgccat aatattgatg
    18001 cagagacaaa taatctattt gatcgtacgc ttgatcaaga actgttaact cccaatcacc
    18061 aaatgtaggc atttgccgat tcgaacttcc gcataaaacc acctcaattg aatcatctac
    18121 gagtttcatc gccttagccg tttcctctgc taaccgtcca tattcttccg ctgttttatg
    18181 tccaatctgc caaggaccgt ccatttcatt ccctaaacac catgtcttaa ttccaaaagg
    18241 gctctcttga ccattcgaac gccttaaatc actccaataa gttcctttag gaaaattaca
    18301 atattcgact aaattacgag ctgcatctac tccacgagta cccagattga cagccatatt
    18361 gatttcggcc cccacatgct gacaccagtt ataaaattca tgaatcccaa cttcattcgt
    18421 ttcaattgtc cgccaagcta aatccaacct tctaggtcta tttgctatgg gcccaatact
    18481 gtcttcccaa cgaaatcctg acacaaaatt tccaccagga tatctaatca tgggaatgtt
    18541 caattctttt accaagtgaa tgacgtcttg gcgaaatcca cttttattag ctgatggatg
    18601 atctggctga taaatccctt catatactgc tcgtcccata tgttcaataa atgaactgta
    18661 caaccgcgga tcaactttgc ctacacgctt gacactacta atcaacattg tggcttccat
    18721 aactgacctc ctttaattta tacatgaata atacacctcg ttgaaagcgt tgtcaataat
    18781 ataaatgtta aacttactta ttttttatcg attttaatta accaaaatat taataatgat
    18841 taaaacaaat aatttaatat aacttttatt aacaaatatg ttaatatata agaaaaacct
    18901 tttaagaggt gatatatatg caactacaaa taaacgaaga atccttaccg gtatatgaag
    18961 cattagctag taaaacaagg atcaagatca tccagctact ttccaaaaaa aagatgaatg
    19021 tcaaagattt ggcaaaagaa cttggagtga gcagtgctat cacgacgatg cacgtgaaaa
    19081 agctagagga agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc
    19141 ttcgtgtcga taaaatcgat atcagttttc cagaaaaaat attcaatgct tttgatacga
    19201 aagaaacatc gatacctatt ggtcactata caaattatgc aattgaacca acatgcggtt
    19261 tagctacgat ccatgatttt atcgggaaag tggatgaacc acgctatttt atggatcctc
    19321 gaaggatgga tgcccgcatt ctctggttta ccagcggatt tgtcgagtat caggcaccta
    19381 attttttaaa tacagaggat acattggaga tgttggaagt ttctgtggaa atttcttctg
    19441 aatttccttt ttcaaatgac aattggcctt cagatatcac tttcagccta aatggtgtgg
    19501 agctaggaac atggacgagc cctggagatt ttgctgatat ccgcggtaaa tatacgcctg
    19561 attggtatcc agataatctt aatcaatacg gcttgctgaa gacgatccga attaccaaac
    19621 acttgacgaa tatgaatgga gaacccttgt caaacatcac gatcaacgac atccctaaag
    19681 agcaagacac ttggcatttg cgaatcgaag tgaaagacga tgccaaacat gtggggggct
    19741 gtactttatt tggtaaagga ttcggtaatt acgatcagga tatcaaatta aaagtttact
    19801 acagctgata tttttctttt ctattttata aaacaaaaac agccaatccc tatgaaggga
    19861 cggctgtttt tgctttattg ttatttccag aaatcatcca aaacggaaat tggcaaatgt
    19921 cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg agcgggaact
    19981 tcttttcctt ctagataatc atcaatttca ttatatgtga cacctagtgc gacttcatct
    20041 gcaatcattg gtttcccatc ttcaagatct gcagtcggaa tttttgtgta taatttttcc
    20101 ggtgcattca atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg gaataatggc
    20161 aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc cagtaatgtt ttctgcagca
    20221 tgatctgtcc ctaatacggc tcctgctctt tctccagcta cggcatattg cgtaatcata
    20281 cgttgacgtg ctttgatatt tcctttgtta aaatcgctga ttcccacacc tgcttcgctt
    20341 aatacttgta cttgtgcatc aacagctggt ttgatattta ctcgcagact aacatcgggc
    20401 tggataaagt caagagcagc ttttgcatct tcttcatctg cttgttcacc ataaggaaga
    20461 cgaacagcga taaattgata gttttcatct tttgtttctt cacgcatttc ttccatcgtt
    20521 agttgcgcca aacggcctgc taaagtagaa tcttggccgc cgctgatacc tagtacaaaa
    20581 gttttaagaa acggatattt tttcaaatat gctttcataa agtcaatact tttacgtatt
    20641 tcttcttttg gatcaattgt tggtttgaca cctaattcat tgataatttc tgtctgcagg
    20701 ctcatttatt ctcctcctat gcttctgtat gaagttctgc tacgcttttt ttcattcgtt
    20761 ccatgatagc cattttatgg ttccagcatt cagtagacag atcgactggg tatttttgcg
    20821 gattcaagtc tcgtttatac tcttcccata gtgaatctag attttctttt gtatattctt
    20881 tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag atatcttgta
    20941 aaactgggcg agctgtaaag tctcgaacag ttttattgat aaacgtatgc actggatgga
    21001 acatgaagat agcttcttct tctcgagggt cttcatccca taatgtcacg tagtctcctt
    21061 cagatttccc atcaaaattt cgagtgatgc gccacacttg ttttttccct ggagtagtta
    21121 ctttttctgc attgcttgat aacttgatcg tatccatcat ttttccttca tcatcttcga
    21181 tcgatactag cttgaatact gcaccaaggg ctggctgatc ataggctgtg atcaattttg
    21241 tcccaacacc ccatacatca attttagctt tttgcatctt taaattcaaa atcgttgctt
    21301 catccaagtc attcgaagca tagatttttg cttccgtaaa tccggctgca tctaattgtt
    21361 cccgaacacg cttagatata taggccatat ctccactgtc gatgcgtacg ccttggaagt
    21421 tgattttgtc acctaattct ttcgccacgc gaatcgcact tggcacacct gatttcaacg
    21481 tatcataagt gtcgactaag aacacgcagt ctttatgggt tttagcgtag gccataaatg
    21541 cttcataatc atttccataa gattggacta acgaatgagc atgggtcccg ctagctggta
    21601 tcccaaatat cttcccagca cgtacattgc ttgttgcatc tgctccgccg atatatgccg
    21661 ctctagttcc ccagactgct gcatccagtt cttgtgcccg tctagttcca aattccaata
    21721 gtggatcttc tccgatgaca gatttgatac gagctgcttt tgtcgcgatc aacgtttgga
    21781 aattgaccat attcaacagt gctgtttcta ctaattggca gtgcgctaaa ggcccttcca
    21841 cttggattaa tggttcattt gcaaaaacta gttctccttc acgagcagaa cgaactgttg
    21901 ctttaaattc aaaattttgt aaatactcca aaaaaccttc aggatagact tctaatccac
    21961 gcaagtaagc aatatcggaa tcattgaacg tcagattctc taaataattg actaaccgtt
    22021 ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag taacattcaa
    22081 aaacagcatg taaatcagct cgacctaatt cccagtatgt ctgcatcata ttgatttgat
    22141 atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt attattcatc ttgtattact
    22201 cctcttggca agttttcatt tcctacccat tataacaaaa aaaattaata ggaaaagaaa
    22261 tcttccattt tttctgaaaa agcacatgtt ttacggatat ttcttgctaa aaatgaactc
    22321 catctgctaa aactatcctt tcttattggt atttaaccta aacgagaaag aatacttatt
    22381 ttattcaatg tatccgagac acgaagaaat tagcgttttc tatttttctt acttgataaa
    22441 ataagtagat tttcctacta caatcgtagc cgttaagata ccgatgattg aacaaatata
    22501 accaaatgtc tctacaacat aatcaatggc aaaagttttt gaagcaataa tacgtctttc
    22561 cttttttgct tcttatcccc aaagaaacgg atataactta ttatgtattg aatttttttg
    22621 gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa actcgcggaa aaaatatcac
    22681 acgtttttaa aaagatacaa agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg
    22741 cgagacatca atcagtctcg ccttgttttt tattgtatac attagatatg gagcaggtta
    22801 ccataaagat gattattagt cgttattaaa tagaatcctt cgtatttttt tgttctatca
    22861 ctacaatatc tttatttgga cgtttgaatt gtattccggc acgttctagc tcctgtttca
    22921 ctcctacatg gactttgaca tcttctcgtg tcatttttaa aacgtgatga atcgcatagt
    22981 cgatttcttg ccttgtgatt tttttcttcg tattgatcat cacgacttgc tttttatcat
    23041 atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa taattaatag
    23101 ccgtattccc aaaaaagaat tccactactt taggataaaa tgtcttaata tttaaattac
    23161 tattcacagg tgtttcgatt agcttcatct cattcctctt ttctatatag tcgatttaga
    23221 tccaatctta agttatgtta ccatctttcc tagcattaag gaatcggtta caattgaacc
    23281 tttaaagaca aaaggattta ccttttatca cttaatagta taacataatt tcgtgagaaa
    23341 aaaatacaat tttttcttga ccatctcgtt acgtaaagga ttatgcttac atttgtcagg
    23401 aggaaaaata atggagtaca caataaagaa aatgtcagag atatcaggag tcagtccgag
    23461 aaccttgcgc ttttatgatg agattggact gctaaaacca gctaggatca attcttcagg
    23521 ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat tttatcgaac
    23581 aatggcattc aagttggatg atatccaaga ggtcttagac aatccatctt ttgaccacca
    23641 gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct
    23701 cttgacaact gtccaacaga cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa
    23761 agaaaaattt gaagggttta aacaacaaaa gttgaaagaa aatgaagaat cctatggaca
    23821 agagattcga caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa
    23881 tatggataaa gaaacgtatg aagaaatgca aactgttgaa aagcagatgt tatttgatct
    23941 cactctttac ttaaaagaac cagaagatga aagcttagca gaccgtatct tccaagctca
    24001 taagaagtgg ttctcttaca gctggcctga atatcagcca aaagcgcata aaggactagg
    24061 aatgatgtac gaagccgatg aacggttcac tgcatattat gacgagcgaa gtggtcatgg
    24121 tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg taatctttta catgatccaa
    24181 caaaaaagta cacgtaaaat gacctaataa caaaacagca gacacccaag ttagattttt
    24241 atgctaactt ttggtgtctg ctccattctt tcacaagttt tttcatccta ctttaaatat
    24301 attcatcgtt gaataaacgt ataaggtatt ttcactttat cctttttttc tgtaagaaac
    24361 aattgaaatg ccatttctcc gcaagcttcc agatggtggt cgatggttgg gaaaccaaga
    24421 agctcgctga tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc ttgcagatag
    24481 ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa agacaccatc aactccaagc
    24541 tctttaaaaa agaagcctgc ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca
    24601 agtgattcat caaaagatgg aaaaagttga tgacatagct gcaaagtgat ttttgagtta
    24661 taacttagcc tgccacttcg ccccaacgtg atgcccaatt gctttatacc ttgctgcttc
    24721 aaataagtaa gaccttctgt aattgattgt tctcgatcaa taaagacaca gcttgcctgt
    24781 tttcccttga tttcttcaca aaaaactaga ggtccatatt tttgatatgc caataaatca
    24841 tctagtgtat tggcacgaga cgtaatgatc aatccatcga aagctttagc tgcaaattct
    24901 tcaagatacc ttcgctcttt ttctttatga tagcccgtcg gtaaaagtgt cactttgtaa
    24961 tcataatcaa acgctgcttc aatgatccca ctgatcaact gatcaaaata cggatgattg
    25021 gtataaggca gaatcactcc aatgcttttc gtttttccat aacttaactg tctggcattt
    25081 gcattaggga tataatcgac ttctttcatg accgcaagta tttctttcct tttttcctca
    25141 tctacatatg gatgtccatt gattacacgt gaaatagtag aaactgaata acctgtgagc
    25201 tttgcgatat ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa agattttctt
    25261 gtaaatagtc taccacagct ggaccgcatt caaaaaactg atacattttt ccttcatgcg
    25321 aaaaactgat ccgctgcccg ccccaatata tatcttttgt caaattttcg atttcattca
    25381 ttggtatttc ttttactata ttcctattca acccttgaaa actgtacaac gttaatacgg
    25441 atcccttgaa ctcgacaaac gcataaaaat aattgttcgt tgtgccatct gttatctcta
    25501 cataagtatt cattgcaagc cctccttttg atactttgag tatagtgtat gaaacgcgtc
    25561 tcatagcaag gagtttgttt tgccaaagaa aagttttatt cctctttcat tattaccaaa
    25621 gtctcttatt ttaaattgaa tacattataa tgatactgta aatatatctt tattattagt
    25681 gaat
//
LOCUS       DAFEHS010000031        25641 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFEHS010000031
VERSION     DAFEHS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.031"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            128..3655
                     /locus_tag="LP327_001430"
     CDS             128..3655
                     /locus_tag="LP327_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBL1725552.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3652..7374
                     /gene="addA"
                     /locus_tag="LP327_001431"
     CDS             3652..7374
                     /gene="addA"
                     /locus_tag="LP327_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBL1725553.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7443..7784
                     /locus_tag="LP327_001432"
     CDS             7443..7784
                     /locus_tag="LP327_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1725554.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8093..9136
                     /gene="pheS"
                     /locus_tag="LP327_001433"
     CDS             8093..9136
                     /gene="pheS"
                     /locus_tag="LP327_001433"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBL1725555.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9141..11561
                     /locus_tag="LP327_001434"
     CDS             9141..11561
                     /locus_tag="LP327_001434"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBL1725556.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11731..12741)
                     /gene="trpS"
                     /locus_tag="LP327_001435"
     CDS             complement(11731..12741)
                     /gene="trpS"
                     /locus_tag="LP327_001435"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBL1725557.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12995..13732
                     /locus_tag="LP327_001436"
     CDS             12995..13732
                     /locus_tag="LP327_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL1725558.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13746..14561
                     /locus_tag="LP327_001437"
     CDS             13746..14561
                     /locus_tag="LP327_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL1725559.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14574..15221
                     /locus_tag="LP327_001438"
     CDS             14574..15221
                     /locus_tag="LP327_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL1725560.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15237..15893
                     /locus_tag="LP327_001439"
     CDS             15237..15893
                     /locus_tag="LP327_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL1725561.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16055..16876
                     /gene="racE"
                     /locus_tag="LP327_001440"
     CDS             16055..16876
                     /gene="racE"
                     /locus_tag="LP327_001440"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBL1725562.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16879..18234
                     /gene="rph"
                     /locus_tag="LP327_001441"
     CDS             16879..18234
                     /gene="rph"
                     /locus_tag="LP327_001441"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBL1725563.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18235..18753
                     /locus_tag="LP327_001442"
     CDS             18235..18753
                     /locus_tag="LP327_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL1725564.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18860..19360
                     /locus_tag="LP327_001443"
     CDS             18860..19360
                     /locus_tag="LP327_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL1725565.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19640..20560)
                     /locus_tag="LP327_001444"
     CDS             complement(19640..20560)
                     /locus_tag="LP327_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBL1725566.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20725..21738
                     /locus_tag="LP327_001445"
     CDS             20725..21738
                     /locus_tag="LP327_001445"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBL1725567.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21848..22603)
                     /gene="fabI"
                     /locus_tag="LP327_001446"
     CDS             complement(21848..22603)
                     /gene="fabI"
                     /locus_tag="LP327_001446"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBL1725568.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22823..23263
                     /gene="fabZ"
                     /locus_tag="LP327_001447"
     CDS             22823..23263
                     /gene="fabZ"
                     /locus_tag="LP327_001447"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL1725569.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23378..24139)
                     /locus_tag="LP327_001448"
     CDS             complement(23378..24139)
                     /locus_tag="LP327_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL1725570.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24328..25422
                     /locus_tag="LP327_001449"
     CDS             24328..25422
                     /locus_tag="LP327_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL1725571.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25508..>25641)
                     /locus_tag="LP327_001450"
     CDS             complement(25508..>25641)
                     /locus_tag="LP327_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL1725572.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8493 a         4442 c         5408 g         7298 t
ORIGIN      
        1 gcgtatactt aaaaaagtag aaatatcaga accttaaaag gatataattt gaagaatata
       61 gaacaaatgt tcagtatttt ttgatataat aaaggagcac cgtatcatgt gttgaaggag
      121 gaaaagcatg tctttacaat ttattcttgg aaacgggaaa aaagatcacc gtcaagtctt
      181 gatcgacgaa gcatccgctt ggctcgacca ggatgcaagt aaccaagtat ttttccttgt
      241 tcccaactac agcaaattcg aacaggaaca agaaatttta gcggagatgc gaagaagaag
      301 cggaaaaaaa actttcagca caacaaacgt tcaagtattt agtttttatc gtctggcttg
      361 gtatttgctg caacagacaa ctcttcttac aggaaatgaa ttatcagaat caggatctgc
      421 aatgatcttg cgacagattt tagaaaaaaa tgaggaagaa ttaacaatct ttcgcggaga
      481 aatcaataaa agagggttta ttcgtcaatt acaagaactc tacagtgaat tgcgtacagg
      541 aaatattcaa ccagaagacc tgatgctttt atttggtgat tcacctaatg cagaagacca
      601 gcaacttaaa atgaaagact tgaagctgat tttttcagct tatgacagcg aattgactca
      661 acgtgcacta acaaatgaag atgcactatc tatactagca aattatatgc agacacaaga
      721 tttcaacaac gtcaaattta ttgtcagtgg tttttctcga atcaatggtc aagagtatca
      781 attactgcag gcaatgatgg aaaaggggca attggttgtc gatttattgc tggatcgtcc
      841 ttatacagcg gatcttccag aagtacttac gctctttcat gagactggaa agctttattt
      901 ccgtcttttt caaagtgcaa gagaacggaa agtccctata ttagtggacc gatttgcacc
      961 agagcagact cgtccgacag agcttcaact gctgcaaaca atgtgggaag agacatctag
     1021 ccaaaagaaa gcggcagtac acaaaatagg gggacaagag catctccatt tgtgggaagc
     1081 tgaaaatcca actgaagaag tccgacaatt agctgttgaa atcagacgac tagtcagtga
     1141 agaaaactac cgttatcgtg atatccaagt gctgacaaaa aatatgtctt tatatggaaa
     1201 tatccttcgg ccgatctttc aagaaatggc aattcctttt tatatcgatg aagaacaaat
     1261 gatggaaaat catccgctga tcgaatggat caacagtctg ttcgctcttg accgctattc
     1321 ttatcggcta agtgatatta tgcgtttttt caaaactgaa ttatttctgt cgacggaagc
     1381 agaaaccgaa cttgatgaat ggaaacaaga gcgagatgaa ttcaggaaaa aactggacat
     1441 cactgaaaat gtagcacttg cttacaatta tcaaggaact tattggactc gtgaaaaaga
     1501 ctggcagttt gtggcgtatg attttgaagc agaacagatt gaagacgtac agtatttgga
     1561 agaacagtca aatacgatta gacgattatt tcagaaagct gtaccagtat ttttgaaaaa
     1621 aataaagaaa agcaaaacag gacaagaggc cgcaaatgtt ttttatcaat ttttgatcca
     1681 aagtggcgta cagcaacaat tattgttttg gcgtaatcag gaagtagaca gaggaaactt
     1741 agaagcggca agaaaccatg agcagacatg gggagcgctg attgatctgt tggatgaata
     1801 tgtagtaatt tatggtgaat cggcttttga ctggtcactg ttccaagaaa tcattacaag
     1861 tggtctagaa aatctaagtt atggtaaaat ccctaccgca atcgaccaag tgcgaatcaa
     1921 tcgtttggaa ttagttcgac caaatcaagc tgccgtcact tttgcattag ggttgaatga
     1981 tcaagtgttt ccagaccgaa aagagacaaa aggattgttg tccagtgaag aacgagaagt
     2041 attaaatcag gccttgccag atggacagtt tttattcgac ccatcaaagg aaagtatctc
     2101 ttttgagcct ttccaagcct atcttgtttt tctttccgga acagatcgat tatatcttag
     2161 ttatgcacaa agctatgaca cagatggttc gctgaagatg tctccttatt tgcgtagaat
     2221 cacagaatat ttatctgttc ccgtcgagag aaaagagcat ttgacgatcg aaagcgaccc
     2281 taattgttat gtagggactt acagaagcgg aatcaataca gtcaatagga tttatcgatt
     2341 agctttagat gaaaaaaaac ctgtaccaaa gcactggcaa aagctcaaag aattagtact
     2401 gaattcgtca tggcgaaatt ttgctttaca gatttttgaa agtcaggaac atttaaatgt
     2461 tcctgtttcg atgccagaaa aaatggcgga agagctttat ggaaaagaga tttatacttc
     2521 aatttcacgt atggagaatt tttataattg tgaatacaaa tacttcatgc aatttggttt
     2581 gaaattgaaa gaacgaactg tttatggtct aactccagca gcaactggtg atttttatca
     2641 tgaatcattg gatcgtttct tcaagctact gttttcaaac caattatcct tagtgaatat
     2701 gtccgatgcg cagcgtagag actttactga aaaagtgctg caggaagtat tcggagaact
     2761 tcggtttgag atattggata gttctgcaag aatgaactat atccgctacc agttaggaca
     2821 gacgattcaa aaagtgacat gggctttgca aaaacagagc aaaaatacag gcatgcatcc
     2881 tcttcagaca gagattctat ttggtcagat tgctggagca aaaggtatca caggtctgga
     2941 attaccgttg agtaatggtg gaaaactcca tgttcgaggg aaaatcgatc gaatcgatgt
     3001 agcttctgaa caagaagata cttggctaag cgtcgttgat tacaaatcta gcggacgttc
     3061 attcgatgtc acagaagctt attatggcat ggctatgcaa ttattgacgt atctggacgt
     3121 agcattgatg gatgcggtac gtttgactgg aaaggcggct gttaaaccag caggatcata
     3181 ttatttacat gttcataatc ctgtattgac ggagacagat gatgtagaga agaatacatt
     3241 gaaaaaattt agttatgatg gcgtttttgt cgatgatccc gctcttttag aagtactaga
     3301 tcattcactg gaagcaaaag aacaatcact gatctatcca gtgaaaaaaa atgcgaaaga
     3361 tgaataccaa aaaggtagtt tcagtaaaga caaatttttc acggaagaag aactgcaact
     3421 attcatggcg cataatcggg aaaatatgcg gttagctgga gatgcgatta cttccggaga
     3481 aatcaaacta aatccagctt ataaagataa acagcggatt gcctgcgaat tttgtccatt
     3541 cagaagcatc tgtacgtttg atgtgatgct gaaagaaaat aattaccacc gtctggagaa
     3601 aatagataag gaagaagcac tgcgtagaat attgaaaagg agtgaagaag agtgaatcaa
     3661 attcctttaa aaccgcaaaa tgaacgattc acagatgatc agtggcaagc gatttttgat
     3721 caaggagata atctattagt ctctgcgtct gccggatcag gtaaaactac agtgcttgtt
     3781 cgaagagtga ttgaaaaact aaaaatgggt tttgatatcg atgaattatt gattgtgaca
     3841 tttactgaag ctgccgcaag agaaatgaaa gaaagaattc aagaggcatt acaagaatcc
     3901 gtcaatagcg aaagtgaccc tgtacgtaga cagcatttta ctaagcagtt agttttattg
     3961 cccacagcga atatctcaac cttacatgct ttttgcctga cagttattcg acgttattat
     4021 tacttaattg atattgatcc agtctttcgc atgttaacag atgaaacaga aacgatcttg
     4081 atgaaggagg atgtgtggga cgagctgaga gaagcacttt atgcagaaaa tgatgaacga
     4141 tttttccaat taacaatgaa tttttctaat gatcgttcag atgacggctt gacgaatctg
     4201 gtgttttctc tttatgagtt tgcccgtgcc aaccctgacc cccaaaaatg gctagaacag
     4261 ttaagcgata attatcgttt gcctgagggg ctagcgaaat ctcgattata tcaaaaacaa
     4321 atccgtcctt tagttttggc agatatatac caatgtgtac agttatatga acagatgact
     4381 cagttagcac aaggtgaagg attagaaaaa atgcatgagc aagtagctgg agaacaacag
     4441 cagatcaaga atatctatga agcgttctcg caagatcggt tagaagaagc ttatgctggt
     4501 ttggaacaac taactttttc aacttttaag agcagtcgga aagcagaatt gaaagaaatt
     4561 tccaatgaag tcaagggcat gagggataaa gcaaaaaaac tgattcagca gataagtaaa
     4621 agttattttc ctgtttcccc aagccaaatg gaagaattaa cggacaaagc actaccgtta
     4681 gtcgaagaaa tgacaaaagt cacgcaaagc ttcatggacg gtttcagtat gcgtaaacgg
     4741 gaaaaaggtg tgctggattt caatgatctg gaacatttag cgttgcagat tctcacagaa
     4801 aaaacgaaag atgtatggct gccaagtgaa gcttcaaaac attatcgaaa aaaattcaaa
     4861 gaagtaatgg tagatgaata tcaagacgtg aaccaattac aagaagccat tttatactgg
     4921 ctaagagaac ccgatgatac aaaaggaaat atgttcatgg ttggggatgt caaacaatcc
     4981 atctattctt tccgtttagc agatcctagt ttatttatcg ggaaatacga gaatttctca
     5041 aagaaagaag gcggacgtcg aatcgttttg gctgagaact tccgctctag aaaagaagtc
     5101 ctttctttta cgaatcttat ttttgaacaa ctaatggatc cagcagtggg tcaaatcaat
     5161 tatgacgaag cagccaaact gatccaagga ttttcagact ttccagagaa tgaacagttt
     5221 gagccggaaa tcatgatcta tgaaaaagaa caggaagaat cagagattga gattccgacg
     5281 gatgatatac tggaagacaa aacagaaggc gaacttttta tgacaggact aaaaattcgt
     5341 caattaattg attcctcctt tatgatttat gataaaaaat caaaaaaaag tcgtccgata
     5401 gagtacaaag acattgttct cttgacacct acaaagaaga acaacttaac gattttagag
     5461 attttcaaaa cattggatat tccattagag atgaatgatg ctcaaaacta ttttcaagcg
     5521 acagaaattc gcacgatgat ctcgctgcta caattaatcg ataatcccta tcaagatatt
     5581 ccgcttgcgg ctgtattgcg ctcaccgatc gtgggtctga ttgaaccaga attagccagc
     5641 attcgattag ctaaccgtgc acatacttac tatgatgctg tgcttgctta tcaagcatca
     5701 aacgaagatg aattagcagc taagctggaa cattttggca agcaactaga gcattggcga
     5761 gaacttgctc gccgttcttc gatcactgac ttgttatggg atatctatta tgagacggga
     5821 tatctagagt atgtggtcgg tctgccagca ggggcacaga gacaagccaa tctttatgca
     5881 ttggttgatc gtgccaaagc ttacgagcaa agttctttca gaggattata ccagttcgtg
     5941 cgtttcattg aaaaaatgca agaaaaagat aaagacttag cagaaccagt catttcaata
     6001 gaagataatg ctgttcgcgt tatgacgatc catgcaagta aaggcttgga attcccagtt
     6061 gtatttttgt tagatatgac aaaggagttc aacttacaag acttgcgcaa tcggtatgct
     6121 tttgaagaaa aacttggagc aggaattcgc tatatggatc ctgaaacacg agtgttatat
     6181 gatacgttac cgtttcaagc catcaaattg gcaaagcaaa acaaattgtt gtcggaagaa
     6241 atgcgtaaat tgtatgtagg attgactcgg gcagaacaaa agcttttcat tgtcggttcc
     6301 tataaaaata aagagcaaat gatccaaacg tggtctgaag cagctgatca tgaggagtta
     6361 gtttttgatc ctgcgctacg tttgaagggc agaagcagct tgatgaattg gattggttac
     6421 ggattgatcc gtcatcctga aatgcagaaa tacttagagg aagaaatttc gacttctctt
     6481 cttcaacatt caaatgcaca gttttctatt tcatggatga atcagcaatc gatcattgaa
     6541 caaagacagt tgcttgctga aaaagaactg gttaacttag atcagcagat gaaagaagat
     6601 gaaactttat tagcagattc gttacaaaaa cgtctggcat atgaatatcc ttatcaagct
     6661 tcttcacaaa ccacaagtta tcaatctgta tctgaaatca aacgattatt cgaagatcca
     6721 gatgatacac aagaatcacg tttgactctg gaaagcagtc agaacaaagc agcatcccgt
     6781 caatttcgtt atacgcaaga acaactagca gaaccaaaat ttttgcaaaa agatcggcaa
     6841 gtgtcagcag ctgctgttgg aactgctacg catgctttgt tacaattact gccattagag
     6901 atgccaacta cagagagtat ccatcaaaaa ttacaggaat tggtaaggaa acggttagta
     6961 gacgaaaaag tcgcgaaaaa agtagatgtt tcttctatta tatggttctt tcaaacagaa
     7021 cttggtcaac agctgattgc caacaaagaa aacgtaaaac gagaacaacc tttttccatg
     7081 ctgcttcctg ctgacgaagt atttcaagac tatccaaatc aagaagatga actattaatc
     7141 cacggaatcg tcgatggata cctagaagaa aaagatcatc tcaatatcta tgattttaaa
     7201 acagatttta ttttacatcc ggatgatcca gcagaaatag atgcaatcgt ccaaaaatat
     7261 caaggacagc taagacttta ccaacaagca atgtcagaag ctttgaataa gccagtagaa
     7321 aatgttttcc taattttgct tcgagtgaaa caaattataa acattaacaa ataattataa
     7381 ttgagcaaac ttgacttatg ttttgtaagg attatgctat actctgggaa gaggtgagta
     7441 tgatggagct aacacagaca gaattcgttt tatgccctaa atttgaaaaa acattctcta
     7501 ttttaggaaa aaaatggaac ggattgatta tcgatgtact actcgaaaac ggtccgcaac
     7561 gctttgttga gcttgcggca aaaatcccga tggtcagtga tcgagtatta gtcgaacgct
     7621 tgaaggaatt agaaaaagaa ggaatagtca ctcgaaactt tgcctgtagc gaaaagagcc
     7681 gttcagaata tctcttgact gaaaaaggag cagacttgca aaaagcgatg cgtcaaatac
     7741 aagtttggag cgaaaaatgg atcacagagg aagaatgcgg ttgattttgt aaaaaaaatc
     7801 ttgtaaacta tagtccatac gaataagtaa agaacgatga cgaaaaagag tagtttggct
     7861 aaaccagttc agggagatct gtcgcagact gaaagcagat cctgtgtttg aaaaatgaag
     7921 ttcactttcc gagttcgtct gataaaatca gaccggcaat tgtcgttaac gaagtaagag
     7981 tgcggtgtaa gtcatttatc cgcaaattag gatggtaacg cgaagacctc gttccttaac
     8041 aggaatgagg tctttttgtt ttgtatttgg aatttaaagg aggaacaagc acatgtctct
     8101 acaagcacaa ttagagagct tgaaagaagc aacgctcgtc aaaatcacag cggcacaaga
     8161 tttaaatgaa ttaaatcaaa tacgtgttga aacgttagga aaaaaaggac cgatcacaga
     8221 agtattacgt gggatgaaag atctaagtcc agaagagcga ccaaaagtcg gaagtttcgc
     8281 aaatgagatc cgagatcttt tgacagaaga gatcgagcga aaaaaacaag tattagaaga
     8341 agaagcgtta aatgcagctt tggcaaaaga aacaatagac gtgacactac ctggtagaaa
     8401 agtaaaacat ggtacacgtc atatcgtgac acaaatcatg gaagaaattg aagatatttt
     8461 tgtgggtatg ggctatcaga tcgtagaagg atatgaagta gaatctgatc attacaattt
     8521 cgaacggatg aatctgccaa aagatcatcc agcaagggac atgcaagata ctttctatat
     8581 ttcagacgag atattgattc ggacacatac ttcaccagtc caagcacgga caatggaaaa
     8641 acatgatttc tctaagggtg ctctacgaat gatctcgcca gggaaagtct tccgcagaga
     8701 tacagatgat gcgacccaca gtcatcagtt ccatcaaatt gaagggctag ttgttgataa
     8761 aaacatcaca atgggcgacc ttaaaggaac attagaagtc gtaatgaaaa aaatgtttgg
     8821 ggaagatcgt gaaattcgtt tgcgtccaag ttatttccca tttacggaac catcagtaga
     8881 agtagatgtc agctgtttca aatgtggtgg tgccggttgt aacgtatgta aatacactgg
     8941 atggatcgaa attttaggag ctggcatggt acacccgaat gtgttgaaga tgtcaggaat
     9001 cgatccagaa gaatattcag gctttgcttt cggccttgga ccagaccgcg tagctatgtt
     9061 gcggtatgga gtgaatgata tccgtaattt ctatcaaaat gacttacgtt tcttaagtca
     9121 gttcaagggg gagtaagaaa atgttagttt catataaatg gctcaatcgt tatgtagatt
     9181 tatcgaatgt aacaccaaaa gaattagcgg ataagatgtc tgtgacagga atcgaagtag
     9241 agggagtaac ggttccagaa gaaggcttga aaaaaatcgt tgtcggtgaa gtcaaggaat
     9301 gcgtaccaca tccagattct gatcaccttt ctatttgtca agtcgatatt ggcgaagacg
     9361 aactgtctca aatcgtttgt ggagcgccta atgtgaaggt tggaatcaaa gtgattgttg
     9421 cacttcctaa ctcacgtatc gctggtaatg taaaaatcaa aaaaggaaaa atgcgtggcc
     9481 aagtatcaaa tggcatgatt tgctcattac aagaaattgg ctattcagat agtgttatac
     9541 cgaaagaata cgctgaaggt atctattata tgccacaaga agctgtaaat ggcgatccag
     9601 tttttcctta tttggatatg gatgattcga ttattgaatt atctatcaca ccgaatcgtg
     9661 cggatgcatt gtcaatgcgt ggcgtagctt atgaagttgg ggcaatttat cgtcagactc
     9721 ctacattcga tgacgaaaag ctggtagaat cctcacgtcc tgctagcgat aaaattcaag
     9781 tggaagttga agataaagaa gcagttcctg cttatcaaat cagaatcatt gaaggagtaa
     9841 gaatccagcc aagtcctcaa tggctgcaaa atcttttgat gaacgaaggc attcgtccaa
     9901 tcaacaatgt agtagatgtg acgaactata ttttgctttt attcggacaa ccactacatg
     9961 catttgacta cgacaaattg aaatcaaaag agatctttgt ccgccgtgca aaagaaaatg
    10021 aaacattagt aacactagat ggagaatctc gggaattgac aactgacaat ctcgtcatta
    10081 ctaacggcaa agaaccagtt gctttagctg gtgtcatggg agggttagac tcagagatca
    10141 cgaatgaaac aacaacagtt gcactagaat ctgctttatt tgatccaatt tcgattcgtc
    10201 gtacagcaaa acaatttaat ttgcgtagtg aatcctctgc acgttttgaa aaaggaatca
    10261 accatgcgac tgttggagaa gccagtgagg ttgctgcagc aatgatcgtt tctcttgcgg
    10321 gtggggaagt tctacaaggc gcggtcaaag gctcagagat caaagcagaa gacgtatcag
    10381 tgaagatttc acttgaacgc atcaatcaat atttaggaac atctcttgtc gttaaagaag
    10441 taaatgagat ttttgaagca cttggattta gctatgaaga aaaggagtat gtttattccg
    10501 tagtagtacc accaagacgc tgggatatcc aaatcgaagc agatattatc gaagaagttg
    10561 ctcgtattta tggatacgac cagttgccat ctacacttcc aagtggagaa acggttgctg
    10621 gaagcttgtc aaaagaacaa gaaactactc gtaaaattcg tacaatcttt gaaggaagcg
    10681 gattgagcga agcaatcagt tatgcattga caacagaaga aaaatcaaga cagtttgtta
    10741 taaatgaaag taatattaca cgtttggact ggccaatgag cgaagaacgt tctgtattac
    10801 gaatgaattt gatcagcgga ctattagatg atatcagtta taacactgcc cgaaaaaata
    10861 cagagattgc tttatatgaa attggtcggg tgttcttcca agaaaatgat ccacgtacac
    10921 atctgccgca agaagtaaaa catgcagcaa tcgctatttc tggtatttgg gaagaaaaag
    10981 attggcagac gaaagctcaa aatgtagact ttttcaccat caagggtctt ttagaaaact
    11041 tatttgaaca actaggcatc actgaagatg tccattatca aacagtgaca gaaatgaagg
    11101 aaatgcatcc tggaagaact gctgcgattt accttggcga aaaatttatt ggttttgtcg
    11161 gtcaagtgca tccaacgatt acaaaaagtt atgatattcc agaaacatat gttgctgagt
    11221 ttgatttatc tgctatagtg gaagctgctc aaaaagggat cgttttcgaa gcagtgacaa
    11281 aattcccagc agtcagccgg gacatcgctt tgcttgtaaa agaaactgtg acaaatcagg
    11341 aattaacaga agttatccgt tctgctgccg gaagattttt aacagatatc caattatttg
    11401 atgtctatca aggagaaaac attgaaaaag gacataaatc catggcgtat agcctgacat
    11461 tcgtcaaccc agaagcaaca ttgacagatg aagaaatcaa taaagcaatg gaaaaagtcg
    11521 aaaaagcgtt ggtagaaaac ctagaggcaa gtattcgcta agaaggaaaa tatagtaagt
    11581 aacgatcaag tagcttttga agaccataca tttgaaaaaa agatgtgaca caacttcgtc
    11641 acatctcttt ttttgatttt aaaaaaggaa tagcacagag acgagataaa aaaacaggcc
    11701 tttacagcct gcttttttag agtaaatgaa ttaatggaag taattgattc ccattgctga
    11761 ttttacttca gaaagtgttt gtgcagctac tttagcagct tcttcactac ctttacgtaa
    11821 gatttccata acagctgaag gatctttttc caattcttct cggcgcgcac ggatcggtgc
    11881 aaattcagct tccaatacat caatgaggta tcgtttgatc tttacatcac caagtccgcc
    11941 gcggcggtaa tgagctttca tttcttcaat tgcttcttta tctgttccga atacatccaa
    12001 gtaggtgaag accatattcc cttctacttg tccaggatct tgtacatgga tgtggtttgg
    12061 atcagtatac atactcatga cttttttctg taagacatcg gcaggatcgg agatataaat
    12121 accgtttcct aacgatttac tcattttgcc atttccatcg attccaggta atctacccat
    12181 tccttttggt gggaatacgc cttttggttc caccaagact tcaccatatg tatgattgaa
    12241 gctttgtacg atttcctgtg tctgctctag cattggtttt tgatcctcac caacaggtac
    12301 aagattcgct ttgaatgcag tgatatctgc tgcttgagaa acaggataga taaagaatcc
    12361 tgtaggcacg ctttcaccga attttttttg ttcgatttct gttttgacag tcggattgcg
    12421 ccgaacgcgt cctacgctga ctaagttcaa ataatacatg gttaattcag ctaattccgg
    12481 aatttgtgat tggataaata aagtagattt tgcaggatct aatcccacgg ctaaatagtc
    12541 taaagcaact tgcaagacat ttgatgaaac cttctcagga tttttagcat tatctgtcag
    12601 tgcttgcatg tcagcaatca tgacaaacag ttggttgtct tcatctgcct gcatagccac
    12661 tcgggttttt agagaaccga cgtaatgccc taaatgcagc ttcccagttg gtcgatctcc
    12721 cgttaaaata atatttttca aaaaagacac ctctttacga tttgtaagtc tgagcactta
    12781 atatcttacc gtttttcctg aaaagaaaca acagttccta aggaaaaaac aaggaaagag
    12841 gataaaataa ctaatagatt agaaatggaa gaaatattta tgggagaata aacgttctaa
    12901 gaaactttac aaattactaa actatcagat ataatgtatt ttattataca tctttaaaag
    12961 aaatcttact aaataataat gggagtgtaa ttctatggca atgattaaat ttgatcacgt
    13021 ggataaatat tatggcaagt tccacgcatt aaaaaatatc aatctcgaat tcgagaaagg
    13081 agaagttgtc gttgtcattg gtccttctgg ttcaggaaag agcacgatgc ttcgctgtat
    13141 caatggatta gagacgatct cttcaggtaa gctgctgatt aatgatactg acttgcatga
    13201 taaaaagaca aaactgacag aagtccgaaa aaacatcggc atggttttcc aacatttcaa
    13261 tttgtaccca aataaaactg ttctggaaaa catcaaatta gcaccaatca aagtgttgaa
    13321 gcaagaccag gcaactgcag tcaaaaacgc ggagaaattt ttgaaaactg tcaatatgtt
    13381 agataagaaa gattcttatc cttccatgtt atccggggga caacagcaac gggtggcaat
    13441 cgccagaggt ttagcgatga atcctgagat gctgctgttt gatgaaccta cttctgcttt
    13501 ggatccggaa atgattggtg atgtattaga cgttatgaaa aaattggctc atgacggtat
    13561 gtcaatgatt gtcgtgacac atgaaatggg ctttgccaaa gaagtggcgg atcgtgttat
    13621 cttcatggct gatggagaag tattggaaga cagcagaaat gtgagagact ttttcgaaaa
    13681 tccaaatgaa gtccgagcac agcaatttat cagtaaagtt atcaatcatt gataggagga
    13741 aaaagatgcg caggttaaaa aaaatacgcc tgattttcct tttaggcatt gcttttcttg
    13801 cactagccgg atgtaaagga aatgggttgg caaatgaaga tattttagag cgaagcaaat
    13861 ctaccaatga aatcatctgg ggtgtcaagt atgatactcg attatttgga atgatggata
    13921 ttgagagcag aactgtacaa ggatttgatg tagatattgc aaaagcaatc actaaaaaga
    13981 tccttggtga caatggaaaa acagaatttg ttgaagtcac ttctaaaact cggattccat
    14041 tattgaaaaa cggaaatatc gatgcaatta ttgcaacgat gacgatcaca gatgaacgaa
    14101 aaaaacaggt tgacttttca gatgtctatt tcgatgctgg ccaagctttg ttggtaaaga
    14161 aaggaagcca aatcaaaagt gtagatgatt tgaatgcttc tacaacggtt ctagcagtca
    14221 aaggttctac atcggctgcg aatatccgac agcatgcacc agatgcaaaa attttagaat
    14281 tagaaaacta tgcagaagca tttactgctt tacaatctgg tcagggagac gcgatgacta
    14341 cggataatgc gattttgtta gggattgcag atgaaaatcc agagtatgag ttagttggtg
    14401 gaacgtttac taatgagcct tacgggattg cgatcaacaa agggcaagaa aacttcttga
    14461 aagctgttaa ccaagcgcta gaggaaatgc atgcagatgg cacatacgat aaaatctatc
    14521 aaaaatggtt tccaaatgaa actgaaggaa aagtagagta agggggagag gcaatgagcg
    14581 aattgataca aacttatgga ccaatctttg tcgacgggtt caagatcaca ttgttttcga
    14641 gtgtcttagc acttgtgttt agtttgatta tcggaacgtt aatggcaatc ttccaattat
    14701 caaagaataa agtagtgagc gccttagcaa aggcatatgt agaatttttt agaaacattc
    14761 cattattaat catcgtgatg tttttttatg ttgtcgtacc gatttattgg tttaatctag
    14821 atggattcca agcaggtacg attggattaa cgatctatac atctgctttt attgctgaaa
    14881 cagtacgttc agggattcaa accgtaccaa aaggtcaaac agaagcagga ctatcttctg
    14941 gttttaccta ttcagaaatc atgcgttata ttgttttacc gcaagcattc aaaatcgtta
    15001 tcccgccatt aggcaaccaa tttatcaacc tcgtaaaaaa ctcatctatt ttagcaatcg
    15061 tagctggttt agatttgatg tatcaaggag acttgatcgc tagtgaaaca ttcaatacct
    15121 tcgatacgta tatcatcgtt gggttatttt acttagttat cacgctaccg ttatcttact
    15181 taatggttta tcttgagaaa aaatgggcgg ttcgttcata gaaggagggg aaatacatgg
    15241 attttagcgg cgctttttca tggatgaata tccgtttttt attggaagga ttaaaggtta
    15301 ctgttgaagt ttcattgttt tcgatcattt ttagtttctt gatcggtggt ctgacaggtg
    15361 tgattcgttt tgcgaatatc cctttctttt ccaaaatcct tggtgtagtg attgatatta
    15421 tacggaatct tccattatta ttgatcattt tcttcactta ctttgcctta cctcaaatcg
    15481 gcattcagat gaacatcttc tggtccgctg tcgcagcatt aacgattttt gagtcagcta
    15541 tgttgtcgga aattttccga gctggactca atgctgttcc aaaaggacag atggaagcag
    15601 gattatcaac gggactgact tatgtagaat cgatgcgaac gatcgtgtta ccgcaagcat
    15661 ttaaatcaat gatcccggca attgtgagcc aattgatttc actgatcaaa gatacttcat
    15721 tagcagtaat catttcttta cctgaacttt ctcatcaggc acgtattatt tatggacaga
    15781 acaccaatta tgtgcttccg atgttcatca tcatgacact tatgtacttc gtggtatgtt
    15841 acgcgctttc tctgctttcc ggttacttgg aaaagagacg gtacagttat taatagatga
    15901 ttcttttagc ggttgtggca aactaaagtc acaaccgctt ttcctctttc cattcgaaaa
    15961 aaagagattt cgattgtttt aagcaagtaa gagcacttga aaattcacgc taaaaagtac
    16021 acaataggga ggatagaatt tattgaaaaa aaggatgata cgattgacag ataatcgccc
    16081 tatcggattt attgattcag gtgtcggcgg cttgactgta gtaaaagaag ccctgaaaca
    16141 attaccgaat gaaaatattt tatttgtagg agacacagca cgctgcccat atggccctag
    16201 acccgcggaa caggtaatcc agtatacttg ggaaatgacg gattatctgg tggagcaagg
    16261 aatcaagatg ctggtgatcg cctgcaatac cgcaactgcg gtggctttag aagaaatcaa
    16321 agctgctctt tctattccag tcatcggtgt gatccttccc ggtactagag cggcagtaaa
    16381 aaaaacacaa aataaacaag ttggcattat cggtacgatt ggtacggtaa aaagtcaagc
    16441 ttatgaaaaa gcactgaaag agaaagtacc agaattgact gtgacaagtc ttgcttgtcc
    16501 caaatttgtt tcagttgtcg aaagtaatga ataccattca tcggtggcga aaaaaattgt
    16561 ggcagaaaca ttagctcctt taaccactaa aaaaatcgat acattgattt tgggatgcac
    16621 ccattatcca ttattacgcc ccatcattca aaatgtaatg ggagaaaatg ttcaactgat
    16681 cgattctgga gcagaaacag taggtgaagt atctatgctg ttagattatt tcaatttgag
    16741 caattcaccg caaaatggtc ggacattatg ccagttttat acaactggct ctgccaaact
    16801 tttcgaggaa atagctgaag actggcttgg aatcggacac ttaaatgtag aacatatcga
    16861 attgggagga aaataaggat gcgtcatgac gggagaaaag cccaagagct tcgcaaactg
    16921 gagcttcaaa caaatgtttt caaatatcca gaaggatcgg tagtgatccg ttttggtgat
    16981 acaacagtta tttgttctgc aacaatagaa aattctgttc cccctttttt gagagaaaca
    17041 ggaacaggat gggtgacagc agaatatagt atgctgcctc gagcaacgag tacgagaaat
    17101 cgtagagaaa gcagcaaagg gaaactttct ggtagaacaa tggaaatcca gcgtcttatt
    17161 ggtcgttcct tgcgtgcagt agtagacttg gaaaaattag gagaacgtag tatcatcgta
    17221 gactgcgatg tgatccaagc ggatggcggt actagaacag caagtatcac tggggcattt
    17281 gtggcattga aattagccat tgaaaaactt ttaagagaaa aagaattgtc agaagatcca
    17341 ataaaagaac atcttgctgc agttagcgta ggaatcttac cagatgggac ttgcgtcaca
    17401 gatttggatt accaagaaga ttccgcagca cttgtagaca tgaatcttgt aatgacagaa
    17461 tctggaaaat tcgtggagat tcaaggaact ggagaagaag caacgtttga tggtgaacag
    17521 ttgaatgaaa tgctcttttt tggaaaaaac gcgattgaag atttgatcaa agagcaaaaa
    17581 catgctttgc taacagagtt tgctcaaaat gatgagcgga tagaagaaac aaaaacaatc
    17641 atcattgcga caagaaatcc aggaaaagca gaagagtttc gaaatatgtt caaagaagcg
    17701 ggatatcatg taaaaacatt acttgactat cctgaattgc cagatgtgga agaaactggc
    17761 agcacatttg aagaaaatgc ccgattaaaa gcagaaacga ttgcacaatt gcttgatcag
    17821 ccagtcttag cagatgattc aggtttgaaa gtggatgctt taggcggtat gccaggaatc
    17881 tattctgcac gttttgcagg ggaacaaaaa agtgatgcgg ggaataacgc caaattatta
    17941 tatgaattga cagatgtgcc ggatgaaaaa agaacagcac aattccattg tacgttagta
    18001 ttcgcggcac ccaaaaaaga cagccttgta gtagaagcgg aatggcccgg tcgagtagcc
    18061 cgtataccat ctggcgaaaa tggctttggg tatgatcctt tatttatccc agaaggtaag
    18121 aaacaaacag cagcagaatt atcgagcgaa gaaaaaaaca agatcagtca tcgggctcaa
    18181 gcaatgaaaa aattgagtgc cgagtggaaa caatggctgg aaggagagag atagatgcgg
    18241 tatttagtag taagtgataa tcatggagac agagatatcc tcgttgactt agtagatcgt
    18301 tatcgttctg aagtggatat ctttttccat tgtggtgatt cggaattaga agcttctgac
    18361 ccattatggg atacattcca agtggttcaa ggtaattgtg attatggacc aggattcgaa
    18421 cagaaaaaag tgatccaaac tggtcaggat acagtattca tgactcacgg tcatttatcg
    18481 aatgtccgat ttggtttgac acagttagca atcgaagctc gagctgcaac tgccgatatg
    18541 gtgttttttg gtcataccca tcaaataggc tgtgagatgg atcagaaaat cctgtttttg
    18601 aatcctggta gcatttctca gcctagaggc ccgatccaaa tcccatcata tgctcttatc
    18661 gaaagtttag atgaccaaat ttctgttcaa tactataacc gtgcccatca ggcagttgaa
    18721 gagttggcct tttctttttc gagagagcgc tgaataaaaa aactctaatt tttaataaaa
    18781 ataagcttta ttttatgaat tttgcatccg gtttcggtaa aatagtagtg ttgaatgaaa
    18841 tagaaatgga ggccatcaga tgattggacc tatagtaaga gaaatgctgc tggaaaatca
    18901 ggaaacgttt ttgattcctg cagaaaatgt ggcaaatgtt atgtatcatc atccattgtc
    18961 ccatggttta cttgtgttat cgaaggtcgg ttattccaaa attcctgtat taggaaaaga
    19021 tgatcacttt gtaggactag taagtttatc agatgtggtc aataaaatga ttgacttgca
    19081 aacaatcagc atggacccgt tagaaggact aacggttgct gatgtgacag aaacagatgt
    19141 tccgacaatt gatgaaaatt gggaactaga agaagtactc catttgttag tcgattcgcc
    19201 ttttttaccg gttgtcacag aagatcggat tttcaaagga atcattacaa gaaaagagct
    19261 gcttaaagcc gtaaacttca tggtgcatga attagaacgt cgtaatatat tgtctccgaa
    19321 aatcgaagaa ttaaaagaac aaatcgatat cgtgagctaa agaacgagaa actaaaagat
    19381 aatcaaaaaa taagtagaaa aaacagcgta ccagttttga ctttatcttg aaaaagaggc
    19441 tgggacaaaa gcgtttaact ccaagaaata agaaggaatt cacgaaaatt gcttttcaaa
    19501 tttttgtgaa tttcagctta tttccgaagg agttgcttct gcttccgccg tttatacgtg
    19561 tttagagcgt gggacaaaag tgttctttac ttttgtccca cgctcttttt agtgagattc
    19621 gttgcatcgt ttgtccaaat tattttccga cagcaattgg actgttcgga atcgtgaaac
    19681 cagaagctgt caattctttg acataggagc tcaagaactc ttcttttaag gcatattgtt
    19741 ttccgttcaa tgcgtagcag accgtacgaa tcgcaaaatt gctgtttcca agatccacta
    19801 atccaaagat agaaggttca gtttggagct cttcttggta tttttcagct aatgtacgat
    19861 ttactcgatc aatgatctca tagattttgt catatccttc ttcaggaaca atccgaatat
    19921 ctaaaagtac ttgcatattt gcacgagaga gattgctgat cgtggtgatg tttcgattcg
    19981 ggatgaaatg gacagtacca tccgttgctt tcagctgaag ggtacggata ccaacagata
    20041 cgacggttcc ttcaatcgtt aagtttgcta gcttgatgta gtccccaaca tccatctgtt
    20101 gttccatgat aataaaaaat ccggtaataa catcactcat gaacccttgg gcaccaagac
    20161 cgattgccac acctgcaatt ccggcaccag ccaaaagtga tccgatcggc acaccgatag
    20221 cagataaaat tgcatagata aaaaagaaac caagggtata atgaaaaata gtatttaaaa
    20281 gactatgtaa agtctttaag cggctctcgt ttagaatggc ttttttagaa tactgattaa
    20341 aagacttatc aatcagatat tttcccacac ggtgaagaat ccaaaaaagg acgatcaaaa
    20401 aaatcagata taaacttttt tggataaagg cagccaaaat ggcgtcccaa tcaatcccgt
    20461 cccaaaaccg ctgaaacgca tttactttgc tgacagcttg atctgtcaat gtctcgccag
    20521 aagagtctgt cgtaccggca gttgctaata atgaaaacat aaaatcctct tttctaaaat
    20581 ttgattcttt ctgtattgta ccaagaagtt ttcaaaaaag gaaatagcaa aaataactat
    20641 aatgaaggaa aaaagctgaa aataaaatga caaataatat ggataatgat aaaatgtgga
    20701 gaaaataacg aaggtggtaa gtatatgcaa atcgattgga acaatctagg tttttcttat
    20761 atcaaaactc cttggcgttt tattgccaaa tggaaagacg gacaatggga agaaggagaa
    20821 ttgacagaag ataattacct gacgattcat gaaggctctc cagcgcttca ttatggtcag
    20881 caatgttttg aaggtctaaa agcttatcgt cgaaaagacg gcaaggtcaa tctctttcga
    20941 cctgaacaaa atagtcgacg gatgaatcaa agtgctagac gattgctgat gccaatggtc
    21001 cctgaaaaga tgttcgttga tgctgtcaag caagtggtaa aagcaaatga agattttgtg
    21061 ccgccctatg gaacaggcgc tacattatac ttgcgccctt tactgattgg ggtcggtgaa
    21121 aacattggtg tccatccagc tccagaatat ttatttgtcg tcttctgtac gccagtagga
    21181 gcgtatttca aaggtggact aaaaccaacg aattttattg tatctgatta cgatcgtgct
    21241 gctcctaagg ggactggtgc agcaaaagtc ggcggaaatt acgcagcgag tttacttcca
    21301 ggaacagaag caaaagaact ggattactca gattgtgttt atcttgatcc agccacacat
    21361 acgaaaatcg aagaagtagg tgcagcaaat ttctttggaa ttacaaaaga cggaaaattc
    21421 atcactccgc aatcgccctc tatcttacca agtatcacga aatattcctt gttgcagtta
    21481 gctgaagaac gactaggact gcaagcaatt gaaggagata ttttcattga tgatctagat
    21541 caatttgcag aagctggtgc atgtggtaca gcggctgtta tttctccaat cggtgggatt
    21601 tatcacaata acaatcttca tgtattttat agtgaaacgg aagtaggacc agtaacaaaa
    21661 cggttgtatg atgaattgac aggtattcaa tttggagaca tagaagcacc agaaggctgg
    21721 attgaagtcg tggaataatt tcaaagtaat gagttgaaaa aaacgtaaaa agaaaaaatc
    21781 aggaagagac tttaaaaagt tttttcctga tttttatatg ttttacttta aagaatgcaa
    21841 caaaagttta tgaaagatgg acacctttgt caacgtagat aatatcaccg atgattccgc
    21901 tggaaagtgg gctgattaag aaagcacaag cattaccgac ttcttcgatc gtcacacctt
    21961 gctgatcagg cgtacgagat tcagataact ggatcagttt ttgatagtct ttaacacctg
    22021 tgacagctaa tgttttgatt gccccagctg agattccatt gacccgaatt cctttaggcg
    22081 agaactcagc tgccaaataa cggatagcag cttcaagaga agctttcgca atacccatca
    22141 tgttataatt cgggattgcg cgttctgaac ctagataagt catgctaacg atcccgctgt
    22201 ttggtgcaag aaacggttgt gcgtattttg atacagcaat taaagagtag ctgctgatat
    22261 cttgagctaa ttgatagccg tctctggaaa tatcagacac atttccggac aattcttctt
    22321 tgttggcgta agcaactgca tggacaaggc cgtggatctc cccagcatat tcaccgatgg
    22381 cattcattgc tttttcgatg ctttcgtcgc tagaaacatc acattctaca gtgaatgctt
    22441 cttcgccgac caatttgact aatgattttt tcattcgttc attttggtaa gtatagataa
    22501 tttctgcccc ttgtttcaac attgcttcag cacaacccca agcaatgctt tttttgttag
    22561 cgacgcccat aatgacgatt ttttttcctt ctagaaatga cataatgact ctcctttgtt
    22621 tctgataaaa atttgatagt cgtaaaaaaa tagagaataa tagctaaaat aacttcttga
    22681 tttcttaatt attagtcaaa atgttttgat tgtcaaacta ttttttctta aattaaaaaa
    22741 agattgctaa atatttagga atatgatact ttgatagtca aagtaatgaa aatggcaatt
    22801 gcctgaaaga aggagtttaa ttatgtctta cgtattatct gctactgaag taatggaatt
    22861 gattcctaat cgttatccta tttgttatat tgattatgta gatactttag aaccaggaaa
    22921 aaagatcgtt gcgacaaaaa atgtaacaat caatgaagat tttttccaag gtcactttcc
    22981 tgacaatcca gtgatgccgg gtgcgttgat tctagaaaca ctggcacaag caggttcgat
    23041 acttatccta aaatcagaag agtttctagg aaaaagagct tatatcggtg ggatcaataa
    23101 ggccaaattc cgtcaaaaag ttgttccagg agatgtgctg aaatgtcatt ttgaaatcac
    23161 caaaatgaag ggatcagtcg gtacagcgtt atctgcagca tatgtagatg ataaaaaagt
    23221 ctgtgagtgc gagttcacgt tcatcgtcaa ccaaaatgct tgactataaa acctgatata
    23281 gcaaaacaaa aggaagctta cttgcttttt atgtaaaaag aagacgcctg aatcctagca
    23341 tacgttgtca tctatgcagg attcagacgt tattttttta tttttgtttt gcttcttgca
    23401 gtatattgat tttatcgatt tttacttctt cttttggttt gtctgaacta tctgtttcga
    23461 tatttgcaat agcatccacg acatccattc cttcaatcac ttggccgaag acagtataat
    23521 ggccatctag tgaaggataa ccgccgtttt tataagcgtc gatgattgct tgaggatagt
    23581 cgtcttttaa aagaccatca tgcatatcat ctgtattttg aacgataaag aactggctgc
    23641 cgttactgtt aggatcttgt gagcgagcca tagataacgc acctcttata ttataaaggt
    23701 ggttgttgat ctctgtttca aatccttctc cccaaatgct ttcccctcca gtgccatcgc
    23761 ctttaggatc gccaccttgg atcatgaaat ctttgatgac gcgatggaaa gtcagtccgt
    23821 tgtagtaacc gtcttttgca tgtgtcataa agttttcaac cgcttttggt gcgtgttcgg
    23881 gaaataattt gattttgatt gtgcctttat tcgtcaccat ttcgaccaga tcttcatttt
    23941 cggcgacatc tttcgtcaat tgtggtaatt ccaatgcgtt caaatcgacg ctactggcag
    24001 tcgtgctttc cgcagtagat gtactttttg aactttctgt agacgaacta gaagaagtcg
    24061 tgttgtttgc agaacaacca gcagcaaaca gcaatgctgc tgctaaacta atacttaaaa
    24121 taactttatt tttcttcata actgttcctt tccaatttac tattttgtta tctttaatga
    24181 taacaaaata gagggaaaat acctacttaa attattggaa tttatcattt attttaaaag
    24241 aaaaagaaaa ggtgtagacc atttactttg tgtttgctat aatgtaagtg gatacaaaaa
    24301 cgaaaagatg caggaggaaa tggtaatatg ggaaaattag gaatctcgat ttatccagaa
    24361 cggtcaacat ttgaaaagga caaagcgtac ttggatttag ctcataagta tgggtataaa
    24421 cgggttttta ccagtttgct gcagatcaat gatgataaag aaaaagttct ttcagaattt
    24481 aaagaagtag ttgattacgc gaatagccta ggcatggaag taatggttga tatcaatcca
    24541 gcgttatttg aacagttgga gatttcttac gatgacttat cttttttcca taagatgggt
    24601 gcctacgggg tccgcttaga tatcggattc acaggagcag aagaagcaaa aatgacgcgc
    24661 aatccttttg gtatcaaaat cgagatcaat atgagttcgg gcacgaatta cgttgacaac
    24721 atcatgagtt attcgccaaa cacggataat ttattaggtt ctcataattt ttatcctcat
    24781 cgatactctg gtttaggata cgaacacttt gttttttgtt cagaaaaatt cagaaaatat
    24841 aatttgaata cgatggcatt cgtcaattcg caatctgctg agtttggccc ttggcctacg
    24901 caagatggct tgtgcacgct ggaagaccac cgagatcttg aaattgcgac acaagtgaag
    24961 catttaatcc ttacaggatt gattgatgat atttctattg gaaatgcgta cgcaagtgaa
    25021 gaagaattga aagaaatggc agaagcattt aacgcggatt atccaacatt gaaagtagat
    25081 acggaagaag gtattacaga aaacgaacga atctgcttgt ttgataacct tcatagttat
    25141 cgaggggatc gctcagaata tatccttcgt tcaacgatga ctcgtgtata ctacaaagat
    25201 aaagatttcc cgcctcataa tacgcgagat atgcatcatg gggatgtact gattgacaat
    25261 gaagggtacg gtcaatacaa aggagaaaca caaatcgcct tgaaggatat gaaaaacgac
    25321 ggacgtgtca atgttgtagg aagaatctcc gatgacgaat tattcctcct tgactttttg
    25381 aaaccatggt caagctttaa attaattgaa aataaaaaat agataattag ttattgcaga
    25441 aataatccgc tagaaatagc cagttatttc tgctttttta ttttgttttt ttataggtta
    25501 tgatttgtca aattaagcta gaataaaatg cttcttgtac atagctcaaa aatatttcga
    25561 ttggtgttct gtaattcaat gattttcttg gaatatgatt acgttgattg ctgacactgg
    25621 aaataaatgt ctgattcact t
//
LOCUS       DAFEHS010000032        25460 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFEHS010000032
VERSION     DAFEHS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.032"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            197..2257
                     /locus_tag="LP327_001451"
     CDS             197..2257
                     /locus_tag="LP327_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBL1725573.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2340..2909
                     /locus_tag="LP327_001452"
     CDS             2340..2909
                     /locus_tag="LP327_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1725574.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3084..3890)
                     /locus_tag="LP327_001453"
     CDS             complement(3084..3890)
                     /locus_tag="LP327_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725575.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4028..5542
                     /locus_tag="LP327_001454"
     CDS             4028..5542
                     /locus_tag="LP327_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBL1725576.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5945..6928)
                     /locus_tag="LP327_001455"
     CDS             complement(5945..6928)
                     /locus_tag="LP327_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBL1725577.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6933..7580)
                     /locus_tag="LP327_001456"
     CDS             complement(6933..7580)
                     /locus_tag="LP327_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL1725578.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7665..8297)
                     /locus_tag="LP327_001457"
     CDS             complement(7665..8297)
                     /locus_tag="LP327_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL1725579.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8313..8864)
                     /gene="coaC"
                     /locus_tag="LP327_001458"
     CDS             complement(8313..8864)
                     /gene="coaC"
                     /locus_tag="LP327_001458"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBL1725580.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8861..9598)
                     /locus_tag="LP327_001459"
     CDS             complement(8861..9598)
                     /locus_tag="LP327_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBL1725581.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9595..10584)
                     /locus_tag="LP327_001460"
     CDS             complement(9595..10584)
                     /locus_tag="LP327_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL1725582.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10842..11384
                     /locus_tag="LP327_001461"
     CDS             10842..11384
                     /locus_tag="LP327_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1725583.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     GTFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11401..12486
                     /locus_tag="LP327_001462"
     CDS             11401..12486
                     /locus_tag="LP327_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725584.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12498..13304
                     /locus_tag="LP327_001463"
     CDS             12498..13304
                     /locus_tag="LP327_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725585.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13305..14132
                     /locus_tag="LP327_001464"
     CDS             13305..14132
                     /locus_tag="LP327_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725586.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14129..15202
                     /locus_tag="LP327_001465"
     CDS             14129..15202
                     /locus_tag="LP327_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL1725587.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15630..18326
                     /locus_tag="LP327_001466"
     CDS             15630..18326
                     /locus_tag="LP327_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL1725588.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18403..18891
                     /locus_tag="LP327_001467"
     CDS             18403..18891
                     /locus_tag="LP327_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL1725589.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18903..19397
                     /locus_tag="LP327_001468"
     CDS             18903..19397
                     /locus_tag="LP327_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1725590.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19418..20227
                     /locus_tag="LP327_001469"
     CDS             19418..20227
                     /locus_tag="LP327_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL1725591.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20224..21045
                     /locus_tag="LP327_001470"
     CDS             20224..21045
                     /locus_tag="LP327_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL1725592.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21096..23195
                     /locus_tag="LP327_001471"
     CDS             21096..23195
                     /locus_tag="LP327_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBL1725593.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23192..24310
                     /locus_tag="LP327_001472"
     CDS             23192..24310
                     /locus_tag="LP327_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBL1725594.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24349..25227
                     /locus_tag="LP327_001473"
     CDS             24349..25227
                     /locus_tag="LP327_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL1725595.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
BASE COUNT         8059 a         4440 c         5390 g         7571 t
ORIGIN      
        1 tagatataac tagatatatt acttaacaaa attaaaaagt acataagtta ttattccgat
       61 tttcttgaaa caataaaaaa tcaaaaaaaa tactctccta atagtccatc ttcttttgat
      121 tttatttata taatgaaatc atcttatgaa aattactact tcatgtttag acaagagtag
      181 ggaggactaa gaataaatgt gttcaaaaag acgttcaaaa caactaaata aacacgcttg
      241 tcgtttagat aaggaacggt cgtgttctga aaaacactac gttaagttta tgatgcgcac
      301 gatgctgatc agtttatctg taggtgtcta tatgcatgta cccgttcagg caacgacaca
      361 aaccatcaag gtcgtacgta cctctgtttc tgagaccact gattccccta tcactattac
      421 tcatactttc actgatgcaa atggaaaaca gctacttcct gcattgactt cctataataa
      481 agtcagttat cctccaacga taaaccacta ctggttaatt cctaacaaaa cattatggac
      541 atcggaaacc ggaagcagtg tgccgctttc tatcttttcc gatatgactg atcctacaaa
      601 aggtgatcag ttaaaacaga tcactgatat tttgaatcat gctttgacag atataaagga
      661 cggtggaagc gcacaagttt cttatgtata tgaaaaagat tattcacagt ttgaaagaaa
      721 gaaaatcgat gttctggcag ggacaaaagt ttcgatatct gatctggtag aaaggctgaa
      781 gaacgcagat ggaacacaag gagatatgtc caaagtaatt tcaaacaatg ggaatatcgt
      841 ggatacgact aatacaggaa catctactat ttccttgtcc tattttgatc caattgcttt
      901 aaaaacaatg actgcatctg ctgtgatcca tgttcgtgct gatcaaacag ctatcaaagg
      961 aaaagatctt cacttaagaa caggagagag ttacgcccct aaagatcttg ttgaatatgt
     1021 aacaaaaagc gatggaactt caggaaataa agaagaaatc aaaatgaacg ggacagtaga
     1081 tactacaact ccgggagaat atcccgttgt attaacttat atagactcga aaacatcaaa
     1141 tgtggcagaa acaacagctg tagtttatgt agaagcatct gctgcttcat cgactgtcat
     1201 cactcatttt atagatagtc aaacaaataa agaacttcaa gcagaaacac atatgagtag
     1261 tgtaaatcgt attcctgatt tgcgcctacc tgctgaattt tctgatcgag tgccaaatat
     1321 agatttatcg acttatgaac aaaatgggaa agtcacttct atttctgatt ggatgcaagc
     1381 aaacggatta gacagaaaaa gtcactggtc ggagatcctt gaagtacttc actcagaact
     1441 gatgaataat aggaagcaat caggagggga aattaccttt acttatgttt atataccaga
     1501 caatcgttca ttagaaggtc atgatcttat tgttcatcca aacgaaaaaa taaacaaaga
     1561 agagttgatc caatcagcaa caaattcctt aggtaaaccc gtaccgttaa aggatgtatc
     1621 ggttacaatc aatgaagtac cattggagga tggttatttg accaaacaaa caggtgattt
     1681 tacgattatg tatagttatc atgacccgta ttcactccaa gaactccatg ctcagtcact
     1741 attacatgtt ttaccattaa acaaacagat agatcaaaaa gaacaaaaaa gacaagaaga
     1801 aagcttgtca gaaaaaaaac aacaaatggc tcatgcagtt tctccaacag ggaaagtaaa
     1861 acaaacaaga agcctagagt tagcaagaat atctagaaaa atctcagaat ctaataaaac
     1921 gataaggcaa gaagctcatt tggccccaca agtttctgga ttggaaaatg aaattttaga
     1981 gcctaaaata aaacaacaat cagaaaaaga atttgttgct aaacagtcgg aagatcagaa
     2041 ggaatcagat gaccagaagg aaacgaagaa aaaacgcttg tcaactattg attccaccga
     2101 tgagcatctg cagccgcaag taccacttcc tagtccggga acacccgcac ctggtggagg
     2161 tattccttct tctgctttag gagatttcat gcgaggtatg gcaggaacat gggtatacgc
     2221 taatcgaaat gaatttgacg cagatgactg gttctgattt atatctctat ctgttatagt
     2281 caaataagta gctgagtcat atgttatttg gcataatgga gacgaggagg gatagaaaaa
     2341 tgttagataa aacgttacct tacgcagaaa tctggatgac aagaccactt caacaaaatg
     2401 ttccaatcac gcctttagcg gaaggttttg aatttcgatt ttatcaaaaa gaagatgaat
     2461 ctgcttgggc ggagattgag acttccgttg gggaatttca aacgacagat gaagcacttg
     2521 agtattttca acaaaacttt gctccttatc ctgaagaact tgaaagaagg atgttttttg
     2581 tagagacaaa aaaaggagaa aaagtagcaa cctgtaccgc ttggcgaaaa aaatcaagag
     2641 agcaagtgta tccggttttt cattggttag cagtgaaacc agattaccaa ggaaaagggc
     2701 tcgcaaaagc attgactgcc agagtcttgc aagaatttcc taccctccat acggacggac
     2761 caatctatct tcacacacag acatggagcc atcaggcgat tgctctttat aaaaaaatgg
     2821 gttttacttt tattgctgaa aatctagacg gcacacctaa tccagattat gaaaaagtta
     2881 tgcaggtttt aagtaaatta gataaataac gaaatggtga aataagtcct cagtattggg
     2941 ataaaaaacg aaatagtcat tcctcatatc ttactttttt gagttaatac tatcggataa
     3001 ctaatggcat gccatacatt caaaaaagaa ggggcagtga caaaagtatt gtcactgccc
     3061 cttctcttgt tttttagcat gttttatcgt ttgatttttt taggaataaa attcttgatg
     3121 aacgtaaatt tttggtaatc gtttcgacta tagaggtaac caaatacact gaaaacaact
     3181 gctccaataa aattaacaaa taaatccttc atagtatcca tgattcccac gtctaagtaa
     3241 ccattttgga tgattgtttt atctgtttta ccatctgcat tttgactttc aatgattgtc
     3301 cgagtgatat ctggaacatg atagacagtg tttccgtctt tacttaactc cacactattg
     3361 atttgctgga tgatcgtatc tttttgcata tcgaattgca gtaatttatc tgctgaaaat
     3421 tccacaaact cccacataac accaactgtc atcgaaaaac agaaggaaac aactgctagt
     3481 aataaaggag tcaagttcat ggtttttgta tttttattga gcaaataaac aagggaaaag
     3541 ccgacacccc cagctaaaaa tccgctgaat gcatgtagaa aagtatccca cattgaaaaa
     3601 tagccataaa aatcacttaa ctctcctaaa actgttgaac taaaaataaa agataacaaa
     3661 atgatttcta gcatatttgg aatctgtatc tttaatatct tttccagaaa ataagggatt
     3721 tcaaataaaa ttaatgtcag taataaaagc attgtatttc cccagtgttg gttccagacc
     3781 tgatccacta gcataataac gacgatgata cgtaataaaa tatagattcc gtttgtgatt
     3841 ctacctgcag tttttgtact gttcgtaaaa aaggttttca aatgattcat aaaataactc
     3901 cttcatgtat atccttaatt atactaagaa gcgtgtgttt accaaagcta tatgacttaa
     3961 agaaaaacag agttgatatt gtgaaaaaaa tcacttaata tagtataagg agaaaagggg
     4021 gatacaaatg aaaaaattag ttgctggttt attaacagcc gttgcacttc ttgctatgag
     4081 cgcatgcggc gcagatcaga aaaatacagc gcagacaagt tctagtacga aggaatctgc
     4141 cgaagtatca tcaggcgctt ctgaacaagt atatacagat ccaaaagaaa tgaaagaatc
     4201 ttatgatgtc gtgattattg gagctggtgg tgcagggatg gcagcagcta tcgaagcaaa
     4261 agatgctggg ttgaatccag taattttgga aaagatgcct gttgccggag gaaacacatc
     4321 taaatcatca agcggtatga atgcttcaga aacaaaattt caaaaagaag aaggaatcaa
     4381 agactcgaat gatgccttct atgaagaaac gctaaaaggt ggtggaggaa cgaatgataa
     4441 agaaatgctt cgatactttg tcgatcactc tgctgatgcg gtcgattggc tagatcaaaa
     4501 cggtatcaaa ctggacaact taacgatcac tggagggatg agtgaaaaga ggacacatcg
     4561 tccgtctgat ggctcggcta ttggtggata cttagtggag gggttattga ataacgtcca
     4621 tgagcgggaa atccctattt ttgtcaacgc agatgtgaca gacattaaga aaaatgggga
     4681 aacggtcaat gaagtgactg tacatgttca aggagaagaa ccaaaagagg tcaccggaaa
     4741 agcaatcgta gttaccacag gcggttttgg tgccagcaaa gaatttattg aaaaatatcg
     4801 tcccgattta gctgattata ttacgacaaa tcaagaagga tcaactggag atggtatcac
     4861 catgattgaa aaattaggtg gacaaaccgt tgatatggat aaaatacaaa ttcatccaac
     4921 tgtgcagcag gatgaggggg tcttgattgg tgaggttgtt cgcggagaag gagcaatctt
     4981 ggtcgatcaa gaggggaaaa gatttgtcaa tgaaatggat acaagggata aagtatctgc
     5041 agcaattaca gcattgcctg aaaaatcagc ttatttgatt tttgaccaag gcgtgcgtga
     5101 tcgtgccaca gctattgaat tttatgacca aaaaggatat gtcacaactg gaaagacaat
     5161 ggaagaatta gcagaaaaaa tcgatttacc aaaagaaaca ctagcaaaaa cagttgatgg
     5221 atggaatacg gatgttgcgg agaaaaaaga tgctcaattc aaccgtgcca cagccatgga
     5281 acatgactta tcgtctccga attattatgc gatcaaaatc gcaccaggaa tccattacac
     5341 gatgggcggt gtgaaaatca atacgaacac tgaagtatta gataaagaaa accagccaat
     5401 tagggggtta tatgctgctg gtgaagtcac aggtggctta catggaaaca accggatcgg
     5461 tggaaattca gttgcggaga tcattatttt tgggcgacaa gcgggcatac aagcagctcg
     5521 atttgcttcg gaaaatgaat gaaatagaac gtaaataaaa agaggttgtg aaatcagcgt
     5581 tttgagctaa taccgagggt cagctgattg aacactgttt agtgaagagg ttgtgaaaaa
     5641 gctgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat gtttttaact
     5701 tggttggact tattaccgca gatgcaagct tttgaacacc gtttattcgg aaaaagagcg
     5761 tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc
     5821 agaagaaatt ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg
     5881 aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt tgatcagcat
     5941 gattttattc agttatcttc aaaatgattt ttccgatgtt tttgtttgct tccatatatt
     6001 catgcgcttg tttgactgcg ctgaattgga atgtacggtc aatgattggc cgaatcttgc
     6061 cattttctag aaaaggccca gctttttgca taaaagtttg tgcgagctct gctttatact
     6121 gatcacttct aggtgtcaat aatgtaccaa gcagttggac acgtttccca atcaatacac
     6181 caaggtctat ctcaggcacg atggtcccac ctagcatacc aatcaagacc caacgtccat
     6241 caacggctat agaagctaaa ttcttttccc agtaagaagc gccgatgaaa tccaaaataa
     6301 catcgactcc ccgaccttca gtgatttttc gtacctcttc ttgaaagtct tgttttttat
     6361 agttgatcaa ttcatctgca ccaagttctt tacaaaaggc gagtttttct tctgatcctg
     6421 cagtaacgat gattttcgcg tgagaaagct gtctagctaa ttggatcgct gctgttccca
     6481 caccacttgc tcctgcatgg atcaagacag tttcgccttc ttgtaatttt ccaagccagt
     6541 aaagtgtttg ataagcagtc aaaaaaactt ctgagattcc agcagcagaa acatagtcta
     6601 gcgtatcagg gagaaggatt gcacgatcgg caggcatcac agcatattcg gcataaccac
     6661 ctagattgac taatccgtat acttttgtcc caggagtaaa agtggagata tcacttttat
     6721 tctcaactac ctctccagct acttcgactc ccaaaatagg gtagggaggt ttcaaagaga
     6781 ctgattctct tttcataata tctgttcgat tgacagctgc tgtatggact ttgatcagta
     6841 attcgccttg ttttggttta ggaacaggca cttcaatttg ttctaataca tctacagaac
     6901 caggatgctt gattgaaatg gcacgcatgg atctattcct cctttgccca ttcttggaat
     6961 gctgtattca actctttgct accttcagaa agtactagag tcgtacgaga aatatccgtt
     7021 tcttccacta gagcggcaag tacagcagca actgtagagc gagtgatcgt ttttgatgcc
     7081 ttctcattag gcttagttaa ttgaatggaa gtaacttctt catcgttagt caatgtgact
     7141 ggttgaagaa tgacaaaatc taagttagtt cgtttcagcc attcatctgc ataatgttta
     7201 gtaatataat aatctgtcat ggactcaggc cattttgctg gttcatctgc ataaacagca
     7261 ctgaccatca gatagcgaga gacatgcgca ttttcagctg cgatcactgt tttgattgcg
     7321 ccatccaagt ctacttgcag aagattcttt ccttgagagc cggcagcaaa aatcaataca
     7381 tcgatgcctt tgaatgcttc ggtcattttt tccacggacc aagttaaatc aaaagagaca
     7441 tacgaaacat tctctttttt tactaatgtt tgggaagcaa catctctcac ccctgcaaaa
     7501 atttcatggg aacttggagc taaatctttt atcaggtgtc ggccaatttg tccgtttgca
     7561 cctacaacaa aaattttcat ttggatcatc ctttcgacgt caaatctttc cattcaatag
     7621 taacaccaaa agagaaaaaa atcagagatc ggctacgatg actatcagtt agtaagagaa
     7681 gggtgcttga agtatcttaa caagacattg catactgctg cagtcgcaag acctgcgacg
     7741 attccttcag gtataccact tgtaaaaata accgctagaa tagctgaata aacagcattt
     7801 aaatttgttc cgatgacttt ggcataatct tgctggaata aaaagtagat catattcatc
     7861 actaaaatcg tattggtcaa tgaaccggaa agtccagcta agaataaaga aacggaatct
     7921 gctttttgtt tcattagttt gcgcagacct ttataaacga aataaggaac caccccaatc
     7981 aagatgcgag gaataaacgc gatcagcaag gctttccagc ttccatgttc tgttccgatg
     8041 actgggataa acggagaaaa gacaaaagac aaaggtgttt ggataactgt gctacggatc
     8101 atgctgatca gtccaaatgt accgccaaga aaagcaccta gtcttggtcc taaaacgatc
     8161 gaagcaatga ttacaggaat atgcatggtt gttgcattga ttggaccgat agggataaaa
     8221 cctaaaggcg taacagctaa taaaatcaaa atagctaaaa acatcgcagt taatgtaaag
     8281 ttttttgtat ttttcataag tactcctttt ttctaagcat ttctttcatt aagagtcagc
     8341 atgacctgtt cgatgatcgt ctgaatatca gctaaggcac cttttccaaa atctccacaa
     8401 gctaaaagag attctcttgg ttcaatttcc tgatagccga cagatctcaa tgtttccaga
     8461 ttacgctgag tgatcgggtt ttgatacata ttcgtgttca tggcaggggc aatcaatttt
     8521 ggtgttccca ctggaagcgc cattgcgact gttgaaagaa gatcgtctgc aatgccgttt
     8581 gctagcttgc cgataatgtt tgctgtagca ggcgcgatga gaaacaagtc agcttgtttt
     8641 gctagttcga tatgattgat tacctgcgga tttgattctt gcatgacgtc tgtatggact
     8701 gcgcgcttcg acagagattg caaggttaat ggcgtgataa aatgtgtgct gcttttagac
     8761 ataatcacat ctacctgggc acctaatttg acgaattgac ttgtaagatc agcgcttttg
     8821 taagcagaaa tacttccagt aacgcctagt aagatctttt tcattttgat tcctccagtc
     8881 taattttctc agtaatcaat gcagcaatct cagatttact ttgagcttct tccactgttc
     8941 catctttaga taagagatag ccgtggtgtg tctcgccatg gatttccgtc aaatcatttg
     9001 cgaaaataaa atcagttttg ttatttatca gactattttt tgcgactgcc agaagagatt
     9061 cctttggtac atctactaat agtttgaaac caatgatttt tgcttttggc tgccattcac
     9121 gtagataaga aatgattttt ggtgttttct tcaaaacgag cacaagatgc tcggtgttag
     9181 aagagatctt tgaaacagtg gtatctgtct gttcattttc cgcaaaccaa ttgtccagtt
     9241 cttcttgcgt tgaagaagtg ggtaggtttt ttgccagctg ttcttctgaa aaactaaaag
     9301 caggagtaaa atcactgact gccatactgt ggataatcgc atcgtagtgt tcttttttcg
     9361 ttagttcttc taattgtaaa aataagtcca gtgcagattc gatcgtataa atcgttatat
     9421 tggagtgtct tttcggtttc agtgcttgtt tcgtagtcac gtaatcgatt gtggtatccg
     9481 gattggcagc taaataatca gctaatgctt gccctaatcg tcctgtcgaa tgatttgtaa
     9541 tagcacgtac ttgatcgatt ttttcagtcg ttcctccagc tgtgattaga atcttcattt
     9601 atggtcacct acatttacta gtgttcttcc ttggtgtgtt ccagcaagta gtttagatgc
     9661 tgtttcaggc aattgatcca gagtaatctc ttggacgacc agttcatctg caatctgcaa
     9721 atccgccatc tgttgccata aagaaagacg ttgatctatt ggtacattga cagaatcaat
     9781 tccaatcaac tggatgtttc gcagaataaa aggcaagaca gtcgcgttta gtttgattcc
     9841 cccagcattg ccacataaag ctactgcacc gttataagag atcaatggaa gaagactgct
     9901 tagctgctcg ccacctactg tgtcgatgac atagtcgatt tgctgttttc ctaaagcttt
     9961 tgttgtctct ggcagaaatt catcaggtcg tacgacttgc aaagcaccta gactttttag
    10021 ccatgtgaca gactcttctt ttctggaaaa ggcaatgatt gactggaatc ctagtttatg
    10081 taacagggct atcgctgtac tgcctacacc gccagaagca ccagtaacga caactttttt
    10141 atcggaggtc ataccttgac ggattaaagc gttcacacac aacatagcag taaaccctgc
    10201 tgtaccaagg atcatcgctt gacgcagcgt catatttttt ggtagaggaa ctaaccagtc
    10261 tcctggtact ttttgatatt ggctataacc gcctggatgg gtgacgccaa gcccaaaacc
    10321 agtagcaagt acaagatctt cttttgaaaa acgtggattg ttactttgga cgacagttcc
    10381 ggataaatca atccctggag tcatcggata tgttcgtatc acctgaccag actcgctcat
    10441 tgccaatgca tctttataat tgacatcaga ataggcaatt tttaccaaga cttcattttc
    10501 agccaactca gtaaatggca gatcactgat tgctgtatgg aaaacaggtt cctttattac
    10561 ctgaaaagat cgaaaagcgt tcattcgtga gacactcctt ttaaaatttt cttttatcat
    10621 accataaagc ctactctttt attcttgttc ttccgtaaat ttagacaaaa attaggtgaa
    10681 aaataaagaa tcaaacaact agaatttatt ccaaaagata aagaaatatt cagcaaaaaa
    10741 aattttacaa agggttgcct ttttcaataa atttcgtata ctaatacgag tggtagtaaa
    10801 caggaagttt actattagaa aacttcgatt cggaggaaat tatggaaatt ggtgagaagt
    10861 taagaaattt gcgcatccaa aagaatttga cacaagaaga actaggcgaa cgcacagatt
    10921 tgtcaaaagg gtatatttcc cagttggaac gagatttaag ttcaccatca atgggaacat
    10981 tcttttctat tcttgaagtc ttaggtgtga ctccagaaga attttttcat caagaagcag
    11041 cgaatctgca agtggtttat tcaaaagaag atcatacggt ttatttcgat gaagaaaacg
    11101 ggtatgaatt ggaatggctc gtggctgatt cgaatgaaaa agaaatggag cctgtcttgc
    11161 tgacttttga aggtgcagga gaatataaga cgtttgaacc ttcattatct gaaacgttta
    11221 tttttgtatt agaaggggaa ttagagttaa gcttaggaga gacggtctat gtggcaaaag
    11281 caggacagtc gatctattat caagcgacaa aacaacatca attaaggaat catctcaaaa
    11341 aacgaacaaa agtattgatt gttgcaacgc aatcatattt gtaatatagg gggtaaaaac
    11401 gtggggaaac caatcatttc atttgatcaa gtagtgaaac gctatgatga tgaaacagta
    11461 ttaaaaaaag taagttttga gatcgaacaa ggaaaatttt atacactgct tggtccttcc
    11521 ggctgtggta aaacaacgat cctacgtatt atagctggat tcaccgaagc tacagaaggg
    11581 gacatctatt ttgaagggaa acggttgaat gatttgccag ctaataagcg acaagtaaat
    11641 acagtcttcc aagattatgc actttttcct catatgaatg tttttgataa cgttgctttt
    11701 ggattgaaga tcaagaaaat gtccaaatca gatatcgaaa agaaggtaaa agatgctttg
    11761 cgtatggtcc agcttcctgg atacgaaaaa cgggagatta gtgaaatgtc tggtggtcaa
    11821 agacagcgtg tagcgattgc acgagcaatt gtaaatgaac cgaaagtatt gctattagat
    11881 gaaccgttat cggcgcttga tttgaaacta cgtacagata tgcagtatga actgcgggat
    11941 ctgcaacaac gattaggaat cacgtttatt tttgttactc atgaccaaga agaagcgttg
    12001 gcaatgagtg atgagatttt tgtcatgaat aaagggaaaa tcgtccaaag tggtacaccc
    12061 gtagatattt atgatgagcc aatcaatcat ttcgttgctg attttgttgg ggagagcaat
    12121 atcgtggacg gtgtaatgat cgaagataat ctagttgagt ttgttggtaa gcaatttgaa
    12181 tgtgtcgatg gcgggatgcg tctaaatgaa ccagttgaag ttgttttacg cccagaggat
    12241 ttgacgatca cgacaccaga taaaggaaaa ctagtcgtaa cggtcgatac tcagctgttt
    12301 cgtggtgtcc attatgaaat catctgttat gatgaacagc aaaatgaatg gatggtccat
    12361 tcaacgaaaa aagctaaaga aggaagcaaa gtaggattgg cctttgagcc agaagatatc
    12421 catgtcatgc gtttcaacga gtctgaagaa gaattcgatg cacgtcttga cagctacgaa
    12481 gaataggggg gacaagaatg aaaacaatgc gcaggatcta ttccgtccct tatattttgt
    12541 ggctcggttt attcgtgatc gcgcctgtat taatgatcat ttatcaatct ttttttgata
    12601 tgaatgggca attcacgctg aataattatc tatcttattt tacgtcgggg acatcgcttt
    12661 ctatgacgat caattctgta tggtatgcat ttttgatcac gtttttcacg ctgatcatca
    12721 gttacccgac tgcttatttt ttaacaaaac tgaagcacaa gcaattatgg ctgatgctga
    12781 ttattttgcc tacatgggtg aatctgctgc ttaaagctta cgcatttatt gggattttca
    12841 gtgtgcacgg cagcgtgaat catttctttg attttatcgg gatcgggccg aaacagatat
    12901 tattcacaga cttcagtttt ctgttcgtgg caacgtatat cgagattcca tttatgatta
    12961 tgccaatttt caatgcattg gaagagatga atccttcttt gatcagtgct agtagagatt
    13021 taggcgcgaa tagctttgag accttccgac gagtgatttt tccgttatca ttaaatggtg
    13081 tgaaaagtgg tgtccaagca gtatttattc cttctctttc tttattcatg ctgactcgtc
    13141 tgattggcgg aaatcgggtg atcactttag gaacggctat cgaacagcat ttcttggtta
    13201 cacaaaactg ggggatgggt tctacaatcg gtgtgatttt aatcattgct atgttcatcg
    13261 tgatgctcct gacaggcgag aagaaaaaag gaggtcgctc ataaatgaaa aaatttaaat
    13321 ggtcgtatct ttatttaggg atcgtctttt tattcttata tgcaccgatc ttctatctga
    13381 ttttttattc cttcaatgaa ggaggaacga tgaacagctt tacaggattt acttgggaaa
    13441 attatgaagc tgtttttgaa gatacacgtt tgatcattat cgtattgaat acgttcatgc
    13501 tggctttctt atctgcattg atcgccacga tcatcgggac atttggggct atgggaatct
    13561 attacacgaa aaaacgacgt gcacggacag cgttgttgag catgaataac atcttattag
    13621 tctctcctga tgttattatc ggtgctagtt tcttgatttt ctttacacta ctaggattcg
    13681 gattaggttt tcagagtgta ttgctttccc atattgcttt cagcattcca atcgtcgtct
    13741 tgatggtctt gccaaaattg caggaaatga acgattcaat ggtcgatgcg gctagagacc
    13801 ttggtgctaa taatttccaa gtagtcaaaa atatcatttt gccttatctt tctccaggaa
    13861 tcatagctgg atattttatg gcatttacct attctcttga tgattttgcg gttacatttt
    13921 tcgtcacagg aaatggcttt acgacgctct cagtagagat ctattctcgt gcaagacaag
    13981 gaatcagttt agaaatcaat gcgctaagtg cgttagtttt cttgttctcc atgatattgg
    14041 tcattggata ctactttatt agtaaagaaa accgtccaaa acggctgaag aaaaacagaa
    14101 gagtacgcga gggggtggca cggctgcgat gaaaaaatta caatctctgt ttttaggtat
    14161 tctcatcatt attgcggtgc tgctgcttgg tgttcgtcaa cttgaaaaaa caagtggcat
    14221 gagtggagca aaggtactta ccatctataa ctggggagat tatatcgatc ctagtttgat
    14281 cacgaaattt gaaaaagaat atggatacaa agtgaattat gaaacatttg attctaatga
    14341 agcgatgttc acaaaaatcc agcaaggtgg gacaaactac gacatcgcta taccaagtga
    14401 atatatgatc caaaaaatga tcaaagaaaa actagtactt ccattagatc attcgaagat
    14461 caaaggatta aataatattg atccacgatt tttagacttg gattttgatc cagataatca
    14521 gtattcgatt ccatattttt ggggaacttt agggatcgta tataacgata aattctttga
    14581 tgcaaatcaa attaagcatt gggatgattt gtggaagccg gaattaaaag acagtttgat
    14641 gctgattgac ggtgcacgag aagttatggg gctttctttg aatagtttag gctattcttt
    14701 gaatagtaag aatatggcac agctgacaga agcagcaaat aaattgaatc atttaacacc
    14761 aaatgtcaaa gcaatcgtgg cagatgagat caaaatgtat atgatcaatg aagaagcctc
    14821 cgtggcagtg acattttctg gcgaagctgc cgatatgatg tgggaaaatg aacatttaca
    14881 ttatgtgatc ccaccagaag gatccaatct ttggtttgat aatatcgtta tgccaaaaac
    14941 cgtgaaaaat aaagaaggtg cctatgattt catcaacttt atgctagaac cggaaaatgc
    15001 ggcacaaaat gcagagtata ttggttattc tacaccaaat gaagaagcaa aagcgttatt
    15061 gccaaaagac atttcgtcgg acgaacaatt ctatccatct gatgacacga tctcccatct
    15121 tgaagtctac aaagatctag gatcgaaata cctaggcatc tataatgacc tattcttaga
    15181 attcaagatg tataggagat aatggcaaaa ctcagtaaga atggggactt atcaacgttc
    15241 taaagagcta aaaatcgtcc tttttctatc tgagttttcc aatcttctac caaaagcgga
    15301 tgaaacgccg ctttttttgt gttattttat tttttctacc acgtttttct accagagaaa
    15361 agtggacaaa accattgtaa aatagggccc gagacgggct agtaaatcta agattgaaaa
    15421 agttacagaa ggataaagta attggaaact atgcaaatat acttaaccct tctgtaatct
    15481 attaataaaa ctaatatatg aatccagttc aatatttgta gctatgaaaa aacataaaaa
    15541 tcatattaga taaaaactaa attggtagat aaattatttt gtcagatatc tgaggcaaat
    15601 attcgactat ttgtgtttca aagtaaaaat tggaatacaa atatagaata gacatattgc
    15661 agccgatagt ttattatgct tttagacaca ttaaactgga ggaaaaaatg aaaaagagcg
    15721 ctaaagatcg agtttttgat gagctgatac gactaacagg gaatatgtct tctgtcactg
    15781 ccgagaaaat ttcgaagaat ctaggtatct ctcgtcagaa tgccagccat tatttgacga
    15841 gattagtgga agataaaaag gtcgagaaat taccgggaaa gcctgttttg tggaagcctt
    15901 tagatgaata tgcggttgta gataatacgg agcaaataaa cgaagccttt cattctgtag
    15961 ttggtcatga cggaagtctg agagaggtca ttcaaaaatg tatagccgct gtcaaatatc
    16021 cgcctaacgg actcagtgta ttaataaatg gagccacggg tgttggaaaa agttttttag
    16081 ccacaaaaat ttttgaatac gctatccatg agcaaattat tgaaaaagat gcaccatttg
    16141 ctatcttaaa ctgtgccgat tatgcggaca atcccgaact gctttcggca acactgtttg
    16201 gatacaagaa gggggccttt acgggggctg aaaaggatac cgatggttta ttggcaacgg
    16261 ccaacaacgg ctatcttttt ctcgatgagg tccatcgcct atcgaaagaa aatcaagaaa
    16321 agctctttct atttattgat actggaaact atcgtccagt cggtgaaaat gtaaattggc
    16381 attctgctaa ggttcgtttt attttcgcca cgacggaaaa aggtgaaaat tatttattag
    16441 acacctttga tcggcgtatt caaattagtg ttatgttgcc aacttttgaa gatcgcccca
    16501 ttcgagaacg attagagctg attcaattat tttttcaaaa tgaagcggat gttttacaaa
    16561 aagatattat tgtttcaaga gaagccttgt ctttaatgtt atctcatccc tttagtggga
    16621 atattggtaa gctgaagaat atcattaaaa tcagttgtgc ggatgtgtat agtcgaacac
    16681 aagaagaacc attaacgatt gggaaaaatg agatcttgat tcagttaaat atgctggaat
    16741 ccgaagtaga aagcttgccg attgctagtt tattgataaa gaaaaatcaa aagataaaaa
    16801 cggatcgaat caaaccgttt gattatgatt cgattttgga aaaaattact cagctgataa
    16861 atgaaggtaa tttcaatgaa aatttttctt cgataaagaa tgaagtacag catttggtgc
    16921 atcacattga taaggattct tttttatcaa tgagtagaaa attgatgatt cagatgttta
    16981 gaaaggtctg ggtatccgtt ttaggaaaaa cgtacggatt gacgacgagt ggacctgttg
    17041 cagaaattat tagcagatcc tatgtcattt ccccaagcta taagacagac ttaaccggga
    17101 taaatcgtac attagctgca aatctacctc ggacgactta cttatgtaat cagttcttgg
    17161 agcatttacc accgctctca gaaaaggacg agaactttct aagactgctt attgtcgtga
    17221 ctttatctga ttatgtagat gaatccatcg agctgaaggg cctgttgctt gctcacggag
    17281 atagtacggc tagcagcatt caagcagtag tgaatcaatt atgcggcaat tatgtttttg
    17341 aagcgttgga tatgccagta gagagtaatg tgtcagagat tgtcgaatcg acaaggaatt
    17401 tcgtgaaacg gcaagtgaat acagatagtc tagtgctgat cgtagatatg ggctctttaa
    17461 atcaaattta cacacagatc aagaacgatt taaaagggga gttacttctt ctgaataatt
    17521 tgacgacttc cattgcactt gatattggct tgaagatgac gatgaatgcg ccatttaaac
    17581 agattgcgga ggatgcagca gacaagtatt caatcaatgt tcaatacttt gaaggctttt
    17641 ctcaaagtag caacattatc atttcctgta tgtcgggttt ggggatttcg gacaaattga
    17701 aagatgtttt tcgtcatcat ttatcggatg aatccatcga agttttcacg aaggactaca
    17761 gagaactacg tgaattaatc gaccaaaatg atgaaactta ttttgacaag acaaaattgg
    17821 tcatcacgac ttcggatctt ccacgttcct tttcaattcc taatattaat gtttatgaca
    17881 tcttagacgg tgatggagca gccaatttgg agcatatttt gagtcctgaa atgaagccgg
    17941 cagaatttaa ccgcttgatt caggagctag taaaattctt ttcgttggaa ggaattgcgg
    18001 accgtttgaa ctttctgaac ccaacgatcg tgatgagtga agtggaaaca gtcatttcta
    18061 aatatgaaaa ttactatcat ttcactttaa atggccgtgt aaaactaaat ctctatatgc
    18121 atacggcatt gatgatggaa cgtttattct tggctcggac agaaaaatct gaagaaccga
    18181 attatgattt gaaaccagat gaagatgaat tttattatgt ttctagaagt atttttcaat
    18241 caatggagat gaaatataat tttaaagtca atgcttatga gctttcgctg ctctatgaat
    18301 tattaggacc gtatattcaa aaataaagtg tctaaaaaag tgtctaatct gtttttttta
    18361 ggttagacac tttttttaat gtgaattaac gtgattaaac caatgttttt aaaagttggc
    18421 acaggaattg cttatataaa agtgaacaga aagaaaggaa ttaaccatat gaagaaaatt
    18481 ctaatagcga ctcacggtca cttagcttca ggctttctta gcagtattca attactgacg
    18541 gggaaggttc aagagattac ggtgattaat gcttatgtgg atgagtgtga ttttgaacag
    18601 gagttgaatg tatttgtaaa ccaggtgaac gagcaggatc agatttttgt ttttaccgat
    18661 ttatttggcg gaagtgtcaa tcagaagatt accaaaacct ttttggagaa agagattgct
    18721 gtaactattt tgtctggctt taatctgcca attctgctcg aaatagtatt ggcaaccaag
    18781 gaattaacaa aggaggatat tcaggaactg gtaaataaat gccaacagga attaaagatg
    18841 accattcttg atgaggtaca tgtagtaaat gaagaggaag atttttttta ggaggaagaa
    18901 aaatgattaa agcagtgcga attgatgaac gattaattca tggacaagta gcgatgactt
    18961 ggacaaagag tctaaattta accgggttag tagtagcaag cgatgaggcg gctaccaacg
    19021 atattcaaaa gatgacattg aagatggctg caccatcgaa tgtcaaagtc attattaaaa
    19081 cagttgatga cgcgattcat ttgttaaaag atccgcgggc gaaggacatg cgcttgatgg
    19141 ttttagtccc taatgtcaaa gatgcggtaa aagttgcgaa agctttccca acggaaatcg
    19201 aattaatgaa cgtggggaat gctgggaaaa tgacaccgcc atcggaagat aagaaaacct
    19261 tatcaaagga agtgatgtta acgacgtcag aaattcaaga cttaaaagaa ttagttgaac
    19321 tttatccaga taccgttttt cagcctacac cggcgatgga aaaacgttca gctatctcta
    19381 ttttgagtga tttttagaaa aaggaaggag aaaaattatg tatgatgcat ttgtagtcgc
    19441 tttggccgtt tttattggag ttgctggaca tgaaatgttc gggatgtcca tgctgagtcg
    19501 tccgattgtt gttgctccgt tagcaggagc gttgatgggg gatttgcaga cggggttgat
    19561 tgtcggggcg tcactagaag ccatctttat gggtgtcgtc aatatcggaa tctctagtac
    19621 ggcggagcct tctttggctg ctgggctggc tacagcgttc gcaatcaaaa tgggaggaaa
    19681 cgtggatgcg attattccca ttgtctttcc actagcagta ttaggattac agttaatgaa
    19741 tatgattttt tcttttgtat gtggaccgat ggctgctaaa tttttcaaat ttgcaaaatc
    19801 tggtgatcaa aaaggaatca tcaatctgca cttctttatg tggtttgtcc actatgcgct
    19861 ttatgctctt attccattct ttgccgtttt atttggagca gatgttgtca aaagcgtctt
    19921 ggataatatt ccagaggtca tcatgaacgg attaacagtc gctggaaatc tgctgccggc
    19981 agttgggatg gcgatgctgt tacggatgct ttggagtaaa aatattgctg tgtggttttt
    20041 ctttggggcc gttgttatgg cgtatctcga gttaccattg attgcaattg ccgcagttgg
    20101 aacgattatt gcaattgtta tagcccaaag agatctacaa ctaaaaaata tatctgttca
    20161 tgcagttcaa agcgatgctg ctgtaaatgc aacgcttgac caagaagagg aggatttctt
    20221 tcaatgaaat tagctgatgg aatttctaaa gatgagaaaa aattggttcg gaagatgttt
    20281 tggcgctcga tgactatgta tgcttcggtg aatccggtga cgatgggcgg cggtggtttc
    20341 gcctattcta tgatgccctt cattaatcat ttctacaaaa gtgaagaaga tcgagctgcg
    20401 gcgctggaac gtcatacaca gtatttttcg acgaccattc cttgcgcgtc ctttgtcatg
    20461 gggattgcgg catcgatgga gaaggaaaat tctgaaaaag ctggttttga cgtggaaagt
    20521 atcaacgcga tcaaaacgag tttgatggga ccattggctg gaattggcga ttcattgttc
    20581 tggggagttt ggcgtgtcgt taccgcggga ctagcgatca acttagccaa tgccggaaat
    20641 attttggcac caattatctt tttagttatg ttcaatatac ctaattactt gatgcgatac
    20701 tatggcggcg ttttaggcta ttcattaggc tctaaatata ttgaaaagct gtatgcgaac
    20761 gggttaattg aaattctgac aaaagctgct agtatcttgg gtctgatcat ggtaggtgca
    20821 atgacgatca gtatggtcaa attcaatacc gttttgagct tttcaatgaa gggcgaagaa
    20881 ctaatgaatg tccaagaaat cctcgatagt atttttaaag gaattgtacc gttattggta
    20941 acattgagct gtttcaaatt actagataaa aaagtcggcg tcatcaaaat tatttttgga
    21001 atggtcgtct tgggaatttt attgtcatta cttggaatcg catagtagga agctgcagct
    21061 actaggctgc agttttttta gcaaggaggg aaaagatgac tattaatgaa gtaatttatt
    21121 cacccaaaca cccatttttt aatgctgagt taaacgacgg aaatgtgact acttcaaacg
    21181 aatacagcta ctatcggaat attcctgatg taaagggatt gaccttcgat gtaagtaatc
    21241 aaaagacatt agcaaacctt gatgtttgcg ggacgattca aacgttgacc ttttatcgag
    21301 aaaatttact aactgaggaa aaaccaggtg tttgggtcaa taaacagctc tctcaaacca
    21361 attcgttatc cttaaaggtt gaagtaaacg ggattgtcta ccatttgtca gaatcggatc
    21421 atcgtattga agtcgatgtg attcaagact gtttgcctag atatattcat cattattcga
    21481 cgtttaaggt cattgtagta tcctttgctc ccatactggc taataagcgt ttaagtatgc
    21541 ttgttcaaca ggtttacatt gtgaatgaaa cacacgaaag ccttcaagtt tcagtgaaag
    21601 atgtgccttc gtatcaagag aagtattcgg atcagcaaaa tgtgttgatt tctcaaaaag
    21661 gaaataagga cacgatcgca caacaggaag tggcaagctt cgccattgct tttgtggacc
    21721 caaatgcttt tgaagaagta atgacttttc aggaatctga tatcgaaaaa tggctgaagg
    21781 aaacattgaa ctattttgct cagctatttg ggcagctgtc aatgccaaac gatagagttg
    21841 tccatctcta tcgtcgtgca ctttatcaaa gcttttctag tttcggtatg aatcgacagg
    21901 gacaggtcgt cggcagcaat tggggcagct atcctgctac taatcgaatt tggaataagg
    21961 atatgtatta cgcatcattg ccattcacca tgtttgaacc ggagttatgt caaaaaacgg
    22021 ttctatggtt tgatcaatat ggcgttaagt ttcctggttc aaaatttaca ggaggtatca
    22081 atcattctct gagtaattcg ttatccagtg cgttactggc tagtctttat tttgaacata
    22141 ctggggatac aaaattcttt gagcggtgtc cgcaggttct actgaatgct tcaagaatta
    22201 ttgaggacat attagctcaa cgtcggtcag gggaaccaat gctttttcca tctgtttggt
    22261 tatcagacgc ctttgcactt ggaaagtatc ataccggcag caatctttgt ctttggaagg
    22321 cctgtattgg acttgcagaa atttttgaag ccctagaaca actgagtctt agcaagcgat
    22381 acaaaaacat tgcctgtaaa ctaaaggaat caatttgtaa acaaatgact atcgatggtc
    22441 cttttggtga gcaatttttg gagggaatcg gtgatgagga gaaaacaacc tattcagtga
    22501 agcactatca aaaaccgatt cttgagcaag ggttaatctt tttaagtgat gtgattgtgg
    22561 atggaaaaat taatttattg atgcatgatg gagaagaatc agacactacg ttgatgcctt
    22621 tttatcaatt tttagataat aaagatcatt tatatcagaa caccatggcc ttttctgcta
    22681 gttctttcaa tccaacctat agcgaggaaa tcaaaggcat cacttgggga ttagaatccg
    22741 gcgccacttt tcctggcttt atcactgttt taatgagcga gctgcacaac cctcaagctt
    22801 tcgtaacccg attagaagaa ctaatttgtt tggcagattt agacggttcg tggtggtggt
    22861 ggccgtatcg cttagaggct caacaaggag aagtcgtgcg agactttggc tgcggcaaat
    22921 gcggctgggc atcgggtctt tttgtcagcc taatgatcag tcaatatttc ggattgaaat
    22981 tcaaaaaagg agtattgcaa attgatcctg ttgataattt aacgttttct tggaagaatt
    23041 tgaagttcgg tcaagccttc atttcgattg aatgtacgca atccgagttg agtatttcaa
    23101 atttgtcaga aaagccattg aaaataagtg atacgaaaaa aattcttcac gtagaaaagg
    23161 gaaaaacgat tcacattcaa aggagaaaaa gatgagtata ctaaaaaata ttacccaatt
    23221 taaaaaaatc cagagtcgag caatcactcc cgaaaatcca acaggcgaga aaggtaaagc
    23281 tgcgatgttg ccaagcgagc taggaccaag ccgcaaaggt cgtggcgcaa ttccgctgac
    23341 ttctggtgaa gaaactgtga ttgcggatat tactggaacg ggaataattc gtcatatgtg
    23401 gatgacgatt cgtgatcgaa cagaatttgg cagttttgtt ttacgtgatt taatcttaag
    23461 aatttattgg gatgggtcgg atagccctgc tgttgaaagt cctttagggg atttcttttg
    23521 taatgggttt ggagagcgtt gcgaggtgaa ctcgttgccg atagtggtta atcctacagg
    23581 gggctttaat tcctatttcg aaatgccttt ccaaacatcc gctaaaatta cgatcgagaa
    23641 tttacatccg aaggatatgt cctcattttt ttatacgata aattacgctt tggtggacga
    23701 attggaagaa aatagcctat attttcacgc aaattggcat cgggaacgca ttaccaaatt
    23761 acaggaggat tataagttaa ttgatcgatt aaagggtcaa ggctattacg tgggtactta
    23821 ccttgcgctt acctgcctag aacgttattg gtggggcgaa ggcgagttta aattctacat
    23881 tgatggggat agtgagtatc cgaccgtaac ctcaacaggt tcagaagatt attttggcgg
    23941 ggcgtgggcc tttcatgagt acgatgagtt cggacgtcca catgcgaaaa actattcaac
    24001 agccttttta gggtatcctt accaatcaaa tagagatgcc acaagagatc gttttgaaac
    24061 gggaaaattg aatgccgttc atgcctttgg tgacgacgca atgccacgac atggtcttta
    24121 tcgttggcat cttcaagacc ccatctgttt taccgaagat ttatcggtca ctttacaaca
    24181 aatcggcaac gatgatgttc gcttatttga gcggcaagac gatgttgcta gtgtcgctta
    24241 ctggtattca acagaagctt caggaaatac taaacgtcat ttcactgatc gagaacgctt
    24301 gccgcgttaa tgaagtgttt ttcacatatt cgaagggagg aacctatgat gatatatggg
    24361 gcaattgaag ctggagggac taaatttgtc tgtgcagtcg gtactgagga attaacgatt
    24421 ttggaaagag tcagttttcc cacaacaact ccagttgaaa caatgaagca ggtcattgat
    24481 ttttttaaac cttttaagga acaattggca ggcatcgggg tcggctgctt tggtccaatt
    24541 gatattcaat gcaaatcaac gagtttcggt catatcacat caacaccaaa acttgcttgg
    24601 caaaatttca attttgtagg aacgctaaag caatatttca atattccgat tgaatggaca
    24661 acagatgtca atgcggcttg ctatggtgaa tatgttgctg gttctggcaa agggttagca
    24721 ggtgttgcat acttcactgt gggaacaggt atcggaggtg gtgccttgtt caatgggact
    24781 ttcgtggaag ggttcagtca tccggaaatg gggcatatgt tgatcaagcg acatccaaag
    24841 gacaattttt caggaaactg tcccttccac caagattgtt tagaggggat ggcagcagga
    24901 ccagcgattg aaaaacggtt gggtgtaaaa ggccagaatt tattggctga cgattcgttt
    24961 tggcagattg aagcctttta ccttgcccaa tgtgcataca atacaacact gatgttttcg
    25021 ccagatagaa ttatttttgg cggtggtgta atgaaacaag aacacatgaa gaaaaaagtt
    25081 caggataaat ttgttgagct tataaatgga tatgttgaaa taccaccaat tgatagctac
    25141 attattacgc ccgaattagg tgataatgcc ggaataatcg gcggattagc attggccaga
    25201 aaagcggtaa gaaataagca accataatta atgaatgttt cattgatact catgtcaaaa
    25261 gtatttaacc gagtaatcaa atgatcaaat gaagttggat aaagcgaaaa tgaattcaaa
    25321 taaaaaggct ctaagtcaaa tcgtgttgtt ttcattaaat cactgaaagg aaatgcttct
    25381 gcgtttcctt ttttcttttt gttgtgcgct gtataccttg aggatattca ctggtggcgt
    25441 acacttaaat agtaatatat
//
LOCUS       DAFEHS010000033        24623 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFEHS010000033
VERSION     DAFEHS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.033"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            168..974
                     /locus_tag="LP327_001474"
     CDS             168..974
                     /locus_tag="LP327_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725596.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            986..2206
                     /locus_tag="LP327_001475"
     CDS             986..2206
                     /locus_tag="LP327_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725597.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGIVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2196..3782
                     /locus_tag="LP327_001476"
     CDS             2196..3782
                     /locus_tag="LP327_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL1725598.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3821..5959
                     /locus_tag="LP327_001477"
     CDS             3821..5959
                     /locus_tag="LP327_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL1725599.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6247..7638
                     /locus_tag="LP327_001478"
     CDS             6247..7638
                     /locus_tag="LP327_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL1725600.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7697..8506
                     /locus_tag="LP327_001479"
     CDS             7697..8506
                     /locus_tag="LP327_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL1725601.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8478..9467
                     /locus_tag="LP327_001480"
     CDS             8478..9467
                     /locus_tag="LP327_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL1725602.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9581..10576
                     /locus_tag="LP327_001481"
     CDS             9581..10576
                     /locus_tag="LP327_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL1725603.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            10580..11722
                     /locus_tag="LP327_001482"
     CDS             10580..11722
                     /locus_tag="LP327_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBL1725604.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            11719..13146
                     /locus_tag="LP327_001483"
     CDS             11719..13146
                     /locus_tag="LP327_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL1725605.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13213..14502)
                     /locus_tag="LP327_001484"
                     /pseudo
     CDS             complement(13213..14502)
                     /locus_tag="LP327_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14682..15092)
                     /gene="tagD"
                     /locus_tag="LP327_001485"
     CDS             complement(14682..15092)
                     /gene="tagD"
                     /locus_tag="LP327_001485"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HBL1725606.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15218..16609
                     /locus_tag="LP327_001486"
     CDS             15218..16609
                     /locus_tag="LP327_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBL1725607.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            16742..17689
                     /locus_tag="LP327_001487"
     CDS             16742..17689
                     /locus_tag="LP327_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL1725608.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(17729..18889)
                     /locus_tag="LP327_001488"
     CDS             complement(17729..18889)
                     /locus_tag="LP327_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL1725609.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            19054..20229
                     /gene="mltG"
                     /locus_tag="LP327_001489"
     CDS             19054..20229
                     /gene="mltG"
                     /locus_tag="LP327_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBL1725610.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            20365..20844
                     /gene="greA"
                     /locus_tag="LP327_001490"
     CDS             20365..20844
                     /gene="greA"
                     /locus_tag="LP327_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBL1725611.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            21012..21743
                     /locus_tag="LP327_001491"
     CDS             21012..21743
                     /locus_tag="LP327_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBL1725612.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            21740..22807
                     /locus_tag="LP327_001492"
     CDS             21740..22807
                     /locus_tag="LP327_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL1725613.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            22814..23446
                     /locus_tag="LP327_001493"
     CDS             22814..23446
                     /locus_tag="LP327_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1725614.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            23586..24251
                     /locus_tag="LP327_001494"
     CDS             23586..24251
                     /locus_tag="LP327_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBL1725615.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            24279..24572
                     /locus_tag="LP327_001495"
     CDS             24279..24572
                     /locus_tag="LP327_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBL1725616.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8393 a         3655 c         4772 g         7803 t
ORIGIN      
        1 cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat
       61 cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta
      121 aaatggcact tgactgtaat tcactgaaat cgcttggagg aacacaaatg tttaaagatt
      181 tgtatttatt tatgaaagac atttatcaga atagaaaatt actgctacag ttttcgatca
      241 atgacttcaa atcaagatat gcaggatcgt ttttaggaat cttatgggcg tttatcaatc
      301 cgctatttac tgtattgatt tattggctgg tatttggtgt tggactgaaa gcacaaatga
      361 tcgatgcaaa atacccattt atcgtatatt tagttaccgg gatgattccc tggttcttct
      421 tctcagatgc atttgcatca actacggttg tttttcggga atatacttat ttagtaaaga
      481 aagtcgtatt caatattcgt atcttgccca cgacgaaaat cctatccaat ctgtacaccc
      541 atttattctt cattctgatt ggcatagctg tcgcgcttat aaatggtata tatccttcag
      601 caatgtcttt acagctgatt tattatttat tttgcatgtg tgctttcttg acaggattga
      661 cttggctgac ggcatcgatc caaccatttt tacctgatat catgcaatta attacgatcg
      721 tgctgcagtt gatcatgtgg acattaccga tattatggag ccctaatcag tttgattcag
      781 gtcttgtaag catattgaaa ttaaatccgc tgtactatgt tgttcaaggt tatcgtgagt
      841 catttttaag tgaagcttgg ttctgggagc atggaacata tactttgtat ttctgggttt
      901 tcacgttagt aatgttcgta atcggctctg cagtgttccg tagattgaaa ccacattttt
      961 cagatgtgct gtaaggagaa tagctatgac agaatatgca attagactaa aagatatcac
     1021 aaaatcttat aatatgtacg caaaaccttc ggatcgattt cgtgaagccc tcaatccttt
     1081 taagaaatcc tatcatgatg tattttatgc gttgaaaaac gtgaatgtag aaataaaaaa
     1141 aggtgagatg atcggtttcg tgggagaaaa cggatcggga aaatctacaa tgttgaaaat
     1201 cattacggga gtactgacac caacatccgg cacgatggaa atcgaaggaa aaatctcagc
     1261 tttgctagaa ctaggatcag gattcaatcc tgagtattct ggctatgaaa atatctattt
     1321 aaatggaata gttctaggat tttcaagaga agaagtcgat gaaatggtcg atgatatcat
     1381 tgaatttgct gatattggcg accatattta tcaacctgta aaaacctatt caagtgggat
     1441 gttcgttcgt ctagcttttg ctgtagcgat caatgtgaat cctgatattt tgattgtgga
     1501 tgaagcattg gcagtaggag atcttgaatt ccaactgaaa tgtatggaaa aatttacaga
     1561 aattaagaac tcgggaaaaa cgattttatt cgtttctcat gatattagtt cgattcgccg
     1621 tttttgtgat cgttcctatt ggttgaaaaa tggagaagtt gtcgaatatg gtgatacgat
     1681 ggaagtaacg acgaattacg aaaacttttt gaagaaaaaa tcagttaaaa cagtagatcg
     1741 aaataaaaac caagtagaac gcttcgcagc tccagatatc gtagacgtga cgaaagccga
     1801 attgttggat aaaaataaaa atccagtgga tatgctggaa caaggagaaa aactatacgt
     1861 taaagtaacc tatgaagtaa aagacgatac aatcaaaaat ccagttttag gaattgctct
     1921 gcgtacagtc gacaattttt atgtgtgcgg actcaatact ctactagatg gtatcaagat
     1981 cccttggaaa aaaggtgaga atatctttta tctggagtat gaaaagttag gtttgttagc
     2041 tggtgaatat tattttgacg ttgcggtctt tgaagaaaat gcaacggttc ctttggtata
     2101 caagacaaaa tacatgaact tgtttgtcag cggttcttat atcggagaag gcatcgtggt
     2161 cttagaccat aaatgggaag aagggactca tagcaatgaa atatgatttt gaaatggacc
     2221 tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat caaaccagga agtaaagttt
     2281 tagagtttgg tcccggaaac ggtcgattaa cgaaacattt gatcggagca aaacaatgtg
     2341 aagtgagcat tgttgagcta gataaggaat tattcgattt tgtcagcgaa tttgcccaag
     2401 atggatttta tggagatatc gaaagttttg aatgggcaaa ctattatgct ggacagacat
     2461 ttgattacgt cttgtttgcg gatgttttag aacatttagt agatcctgga aaaacattga
     2521 agaaagtcag agaatttttg aatgaagaag gagagatcct gattacattc cctaacttag
     2581 cacataattc tgtcatgatc gatttgttca ataatcaatt gccttgggct tcttatggtc
     2641 tcctagatga aacccataat tctttttata ctcatgatgg tttccaaaaa gtttttgaaa
     2701 aagccggatt attcattaat attgaagatt acttgtactt ggctgtagga gatacagaat
     2761 taaaatcaac ttatgaagag ttgccggaag ctgtacgcta tgatttcaaa atgcgaccat
     2821 ttggtgaagt ctaccaatac tttttctcat tgatgaaaca tccagtagta caaacttcca
     2881 ttgcacagcc acaaaactct aattatgtaa aagtactaga agtaacccag caaacgaaac
     2941 aagatgaaac aatccaacaa ataccattca ataatttcac aggggaaaat gagacactaa
     3001 gtttccccgt gtcagaagca acagaaagaa tggtgtttcg cttcgcacag caacctagct
     3061 ttatcgaatt cagtgcagaa gctgctggag aaaaactcat atttatcgat tcaaatgctg
     3121 tagtcaaaac agtaaatgat tgttatttgt ttgatgggaa agaaatacca gagttcgctc
     3181 taacggatat ttctgggaaa gaagtgacta tccattgtca ctatcgcttt atcggggaat
     3241 tgacaccaac aatgaaagaa ttgctggaaa caattcgtcc tatggctgaa gtcacaaagc
     3301 agctttctga gaaaaatgat gaattagaaa gagaaaatca gcatcttctt gaggaaaata
     3361 caagactaga ccatacgtta aaaacaacca caaatcgcta ttgcacattg ttggaaagtg
     3421 atgaatggac gatcaaacat agactaggac gacgtaagaa aaaaacatcg aaaaaaatac
     3481 aagaaaaaga gctttctata tgtattgatg agaaaatatg ggatgcagaa acaaaaatat
     3541 taaaaatcat cggatgggga atcgctaatt cggatcgcca gccattgtcc tataagttat
     3601 cagctgacca atcgccattt ttcgaagcaa ttcctgtttc acgagatgaa gtcaatcaag
     3661 cagaacagct cgcaaaagga acagaagcgg gatttgaatt acgaatcctt tgcgagcaag
     3721 aacgttcatt cttagtggaa gcagtagctc aaaatggtca atcatggatc attgaaatgt
     3781 aaataatgat taacttataa agataaggag taaacgaatc gtgcaaaaaa aagaaattgc
     3841 cgtatttatc gatcaaatca ctcgtgaacg ggccactggc gatttgctta ttgtgggctg
     3901 ggcaattgat gaagtgacca aagaaatccc tacgataaag gtagaaaaag aaaatgtgat
     3961 tgctgaagca acacatgtcg taagattgga tatcaatcat ttatataatc ttgatgttaa
     4021 gacccaaagc ggttttaaga tacgtttatc cgggaaaatg agaggaaaag cgatcttaga
     4081 ttttcaaacg gcaaaacatc aaaatggcat agctgtgaaa ctaaatggta aataccctta
     4141 tgatgacggc attgaatctt catgggaaag aaaaaaaaga ttactgaaaa aagggataaa
     4201 ttatgcaagg actcatggcg taaaaaaaac aatccgccgg gtcaaactgg aattgaaacc
     4261 aggatcgatc gactatgcag agtggatcag tcgtcacgag aaacctgatc tgaaagagca
     4321 aaggaaagaa gcacaagggt ttgcataccg tccattgatt tccatactta tgcctgtcta
     4381 taacgttgaa atcaaatggc tcgaaaaatg cattgattcg gtattggatc aaacgtatga
     4441 tcactgggaa ctatgtatca gtgatgatgc ttctactgat ccagctatcc gaaagtgtct
     4501 ggaatcctat caagcaaagg atgatagaat caaagttgtt ttccgacaag aaaatggaca
     4561 tatcagtctg gcaactaatt ctgcgttgga aatggcagaa ggagaattta tagcgctctt
     4621 agataatgat gatgaattac cgccatttgc attatatgaa gtggcaaaag tgctaaacat
     4681 acatccagaa ctggatttga tctacagtga cgaagataaa atcgatgcag atggcaatcg
     4741 ctttgatccg cacttcaaag cagactggtc tcccgataca ttgatgggaa ataattatat
     4801 ctctcactta ggcgtatatc gaacaagtat tgtgaaaaag cttggcggtt tccgtaaagg
     4861 ctacgaaggc tctcaagatt atgatttggt ccttcgtgtg acagaacaaa tccctgcaga
     4921 tcatatttac catattgacc gagtcttgta tcattggcgg acgatcccag gatcaaccgc
     4981 aagtaatggt gaagccaaaa gttatatcta tgattctgga gtgaaagcat tgacagatgc
     5041 cctgtctaga aggaatatca aaggaagcgt acgtcctggc agaatttctg gattttatga
     5101 gattgcttat gacgtcttgc aagaggattt ggtcagcgtg atcattccaa cgaagaatgg
     5161 ctatgaagat ctaaaaactt gtgtggattc gattatcgaa aaaacgtctt atccaaatta
     5221 tgaaatcatt attgcagata atggaagtac agatcctaaa atgcaggaat tatttgcaga
     5281 atacaagcat cagctgaaag accgatttat cgttgaatta attgacattc cattcaatta
     5341 ttcacgtatc aacaatctgg cagcagaaaa agcaaacggc aaatactttt tattcttgaa
     5401 taacgataca gaagtgattg aacctgactg gatgacagcg atggtttctt atgcacagtt
     5461 tgatcgaatc ggctgtgtag gagcaaaact gttttatcca gatgatacaa cgcagcatgc
     5521 aggagtatta ttaggaatcg gtggtgttgc aggacatgca ttgaataact atgatcgaac
     5581 acattgtggt tatttcggcc gattggtcat tgacgtcaat tacttagcag tcactgcagc
     5641 ttgtatgatg gtcaaggcag ctgacttcaa tgctgtcaat ggatttgatg aaacattaga
     5701 agtagcattc aatgatgttg acttgtgttt gaaagtttat gaactgggtc gttacaatgt
     5761 ctatgcacat caagcagaat tgtatcattt cgaatctaaa tcaagaggtt atgaagatac
     5821 accagaaaaa caaagacgtt ttgccgggga aataaaaaaa atgcaagata agtggccttc
     5881 ttatattgct catgacccat tttataacga taatctaact aaagaaggaa tcggcgattt
     5941 ttcattgaga cctgattgat taaaagaact gtattttcat ttttattgat gaaaatacag
     6001 tttttttagt atatgaaaat tgtaacttgt gaccgaagag atgataggaa gaacacccat
     6061 tcagtaggaa gaatgtgata cgatttttgt ccaactctct gttaggcaga aatatacata
     6121 taaacataca atagtcacct tttctagttt cttttaattt attttacaat ttggttattc
     6181 actaggtttt taaactttgc tatggtaaga tgatagaggc attattttat ggttaggagt
     6241 gtatatatga ataaaaatgg ggagtggaat gcggcacgta ggatattcat tatcatcatg
     6301 gatgtacttg tattgaatgt gtcgatctat cttagctttt tgctgaagtt ccaaggagaa
     6361 gtcccagcaa gaaacttaga gacctttcag cattcagcag tttttatttc cattatcttt
     6421 attgcgttga atattttgtt aggagcttat gtattctata gccgagtgat cagtgatatc
     6481 gtcttcatta ctgttattgg acaggtgctg atagcattgg ggatcatggt agttactttt
     6541 gctggacgtt ggtttgcatt tccgcgtggc gtcatcgtgc tctcgtttat acttggcacg
     6601 atattactta gcgtttatcg gatagttgtt tataaactat acttaagagt cagtcgtgat
     6661 aagaaagtcc ttatcgtagg tttggagaaa gatgtcgcac cagctatcca aaactttcaa
     6721 tctaagaaaa acaacaaaca taaagttgaa gcagtagtga tcgacaagta ttacgaaaac
     6781 acaaaaaaat tgattgatga tatcgatatt gtctacttgg cttctaaaat cccagaagac
     6841 gaaaaaatca atatttatca attagtaact aaaaaagaga agaagctatt tttaaataca
     6901 acttttgaaa atttaacgat gatcaatccg aacatcatga atttcgaaga tgaaagtatc
     6961 attgaagctt ccggtttcaa aattcagccg gaatatgaat tattcaaacg tctttttgat
     7021 atcatcgttt cgcttatttt actgattctt gcttcacctt ttatgctaat cacagcgatt
     7081 ttggtaaaaa caacttcacc tggaccaatt atttataaac aagtacgtat tacgaaaaat
     7141 caaaaggaat tttcaatcta taaatttcgt tccatgagtg taacagctga agcaaaatca
     7201 ggaccggtct tggcaaagag caatgatgca cgtgtgacac cagtaggcaa atttatccgt
     7261 gccgtacgtt ttgacgaatt acctcaaatc tttaatgtat tgaaaggcga tatgtcgatc
     7321 gtcggaccac gtccagaaag accatttttc gttgatcagt tcaatgaaga aaatccttat
     7381 tattatttaa gacacaatgt acgtgcaggg attaccggat atgcacaagt atacggaaaa
     7441 tatgtatcag attataatag taaactacgt tttgatttgt tgtatatcaa aaagtattcc
     7501 ttactattag atttgaagat tttacttcaa acaatcaaaa tcttatttga taagatgtcc
     7561 tcacaaggac tagatgaaga tgaaaatatc cgttcgttag cagaaatcga cttttcacaa
     7621 gagaaaattt atcgctagat gggaaaaatt ttgagcaaag aagtaggaga gaacattgga
     7681 aaataaaaat ataagtatgc tgctttcttt atatgataaa gaaaaaccag agtatttaaa
     7741 ggaagcatta actagtattt ttgagcaaac agtaatgcca aaagaaattg tattagttta
     7801 tgacggccct ataaatgaaa aacttcaagt agttgtcact tatttccaac aaaaagatcc
     7861 tcacttgttt acagttgtaa aattagaaaa aaatcaagga ttggggattg ctttagcaat
     7921 tggattagaa catacgaaaa atcgcttagt agccagaatg gatacagatg atatcatgga
     7981 accccaacga ttagaaaaac aattagagat cttccaaaaa gatccagaaa tagcgattgt
     8041 tgggagtaat attgaggaat ttactggcac gcttagcaat gttatcggaa gaaggattgt
     8101 acctgaatat aatgatgaaa tccgtgtgtt ttcgaaaaga agaaatccct ttaatcatat
     8161 gacagtcatg tttgataaaa atgctatttt agaagtggga aactatcgcc cattaattgg
     8221 atttgaagat tattatttgt gggtaagact gttgaaggcg ggatataaag ggtataatat
     8281 ccaagagatt ctagtccatg ctagagcagg tgctgacatg tatgctagac gtggaggaag
     8341 taaatatctt ttaccaggaa ttaaaggccg atatcgcgta tggaaagatg gtctaggatc
     8401 actaaaagat tttctgatag ttatttgcgg acatcttgta attagtttat taccgaataa
     8461 aattagggga aaagtatatg aatcaaaact tagaaaaaca aactaaactt tctataatca
     8521 taccttttta caatgcaaaa aatactttaa aaagagctgt agacagcgtc ttaacgcaag
     8581 gttatcccaa tattgaaatc ttgttaattg atgatggatc tacagatggt agtagtgaca
     8641 ttggtgacga aattgctaat ctagagagta aagtagcatg ttatcacttg gaaaacgctg
     8701 gtccatcagt tgctaggaat tgtgggataa aagaagcaac tggaaatttg gtaggatttt
     8761 tagatagtga cgattatttt ttacctgatt gttttaataa tgttttgaaa gactaccata
     8821 ggaattaccc cgatatcatt gcttttggat taagtaaagg aaaaaatata gaaaatgcaa
     8881 atttatttgt tcccgaaaag aagattggtt gttcaagtga agaagcaatt gagcaaatgt
     8941 ttagaagtaa ggcagtggat ttttacgtat ggaataaatt ttttaaaagg gaattattca
     9001 atcatatagt ttttccagaa aataaattat atgaggatat ggttcctatg tatgaagctt
     9061 ttagagcggc tagtaaaatt gatattttgc cattttcagg tattttttat tatcaaaata
     9121 atgatagcat cgtttatcag tcttttaatt caaaacagta tgataatatt ttgcaaagaa
     9181 aaatattagt tgatgaagta aagaaagatt atcctcgact tattaatttg gccaatgctc
     9241 gattagtaga cggatacctt tcaacagggt ttaaaattac tggcaagaat caaaatcaga
     9301 aagaaaagca aaaatattat aatcagtcta aaagagaaat aaaaagtata tattcaaaaa
     9361 taagaagtaa caatgagata tcaattgcta aaaaattagc tttattttta tacttaatta
     9421 atcctaaaac ttattttatt ttatataaaa aagttctaaa aaaataaagt ttttttcgat
     9481 atgttatttt ttatgtttat taacttcgga agactaaaat tgttactggt aatgatacat
     9541 ttgtaaaaat aaaaaagatg atttttagga agtgaagaga gtgcataaaa ttagtgttat
     9601 agtacccgca tataacgtag aagactattt agaaaactgc ttagaatcta tttccaacca
     9661 gacacattca aatattgaaa taatagtagt caatgatggt tccccggatc gaagtgggga
     9721 aattgcagaa gaatataaaa agaaagatat gcgattaaaa gtgatacatc aagtgaatgg
     9781 tggtttaagt gcggctagga attctggtct tgattctgca acaggagatt ttgtgtgctt
     9841 tattgacagc gatgattgga tagaaccaga ctatcttgag ttactatatt ttggcacgca
     9901 aacttatgga gcagatatct cggtgctaaa aataaaaaag atttcaagtt tagaacaact
     9961 aaaagaacat acaattgctg agagaaaatg ggaaatattt gatagagaac aagcaatgaa
    10021 aaacttattt ataaataata aaattggcta ttctgcaaat aataaattat tcaatagaaa
    10081 tttatttgat aatataagat tcccactggg taaattaatg gaagataagg ctactactta
    10141 tttgctgatc gatcattccg aaaaagttgc tgtcaattca tctgctaaat atcactatta
    10201 ccaaagccct aaaagtatac tgagaggaag cttcaatcct agaatttttg attcttttga
    10261 agtccatgaa gatatactaa tattcataga taagttttat ccggaattat caattaatgt
    10321 tagaactcgc tatgtctatg aagcaataag gatgatgatg acaatgatta aaaataacta
    10381 taccaataaa gaagattatt tacgttctat aaatatttta caacgctttg aaaaagaagt
    10441 gaaaattgat cccgactttt caccaaaatt aaaaaaatta gtaaagtttt tattaataaa
    10501 taaaaacttg ccttactata tgtcaagtag taagatagca agtaaattct tgattaagat
    10561 agaatctgtg aggtaagaga tgaagaatat aaaaaaattt ttttcaagga atattttccc
    10621 ggtttttatt aaaatattac ctattcaaaa aaatttaatt gtattttcaa gttttggcgg
    10681 gcgtaactat tctggaaatc caagagttat ttcagagaaa attcatgaaa aaaaattgaa
    10741 ttacagaatt atttggttaa tcaaaaaaga agcgattata actgatttac cagattatat
    10801 taaaagaatt gactccgatt cttttttagc tgccttttat ttatatacag ctaagtattg
    10861 gatagacgat agtcggaaaa cgattaagta tgcaaaaagg aaaaaacaac tttattttca
    10921 aacatggcac ggaacgcctt taaaaaaaat tgaatttgat gcaaaagata agttacctaa
    10981 acgttaccta agctatgcga aaaaagatag tgagtcaatt acatatttat tatctggtaa
    11041 tcgctatagt tctaagaagt atgtttcagc attcaatatc gttccttcta aaatattaga
    11101 agtaggaact cctagaaacg atgaattagc ctctagtaaa gagcaagtgt ttgacttgaa
    11161 aaagaaacaa atcaatgtct tatttgctcc aacttttcga aataatattg aggacaacgg
    11221 aattacgcaa ctagaatggt taggtgtgga taacttacgt gaatttttta aaaagcaaca
    11281 acaagaatta aattttatga ctaaatttca tcctaatgtt catagtaaat tagcaactga
    11341 ttctaaaagt atagaatata tgaaaaaaca tcaaattact ttaattaatg atgggattcc
    11401 tcttgaaatg ctttttgaag agatagatgt tttaatcaca gattattcat ctattttttt
    11461 cgattttgcg ctaacaaaaa aaccaattat tctttttaac tatgatgaaa aagaatatag
    11521 cttagaaaga ggtttttata tacagttaag tgagttacca attataagcg tacaagatag
    11581 ttatggcctt gttgatgttt ttaaaaacca acaagaaaag cttttgaaaa gttctcaaga
    11641 gttacttgat tatattggaa attatgaaac aggagcaagt acagaaaagg ttcttgagtt
    11701 gatagagagg gatataaagt gaaaaaaaca gtaaaagata tattttataa tgcgatatat
    11761 caagtctttt tgattgtttt acccttactt actataccta tacttagtcg tagaattggt
    11821 tcaactggtt tagggattta tgggtatgtt ttctcaatat cacagttttt aatgactgtc
    11881 attgctgtgg gaatgaatcc ttttcgtatt cgaaatatcg ctaaatcaag gaaagataaa
    11941 aaagcactat ctttacaatt ctggaatatt tactttattc aatttttaat aggcttgagt
    12001 gtttcctttc tctatatagc ctatatttat ttttttgatg taaagtataa aaatttatac
    12061 ttaattcaat tgatatttat tgtagggcta acattagata tttcttggtt ttttcaagga
    12121 attgaagaat tttcgaaagt ggttattaga aatacagtta tcaaattatc atcggtattg
    12181 ttaataatca tttttgtttc aactaaagat gatttgtgga tttacgtgtt cattacttca
    12241 attactaact ttttaggaag tttagttttc tggttaagta tccacggaaa agtaggtaaa
    12301 ccaatcttta actctaatgt ttttaaacag ttgtggaaac cagcttttat tattttgact
    12361 cctcagttgt ttatgcaagt gtacacaact ttagacaaaa cgatagttgg gtattttgtg
    12421 gatccaacgg aattatctta ttatgatcag tctcaaaaaa ttgctcgtat tatattggca
    12481 gtcttaacat caattactat tgttatgttg cctaaaatat ctaaagcaca ggcagagggg
    12541 aaatcagaaa atattttagt ttacacaaaa aaatcttttg attacacgat tatcattgca
    12601 ttaattcttt ttagtgtggt gtttatcaat acaaaagaat ttgttccatg gttttttggg
    12661 gagaatttca aaccgatgac aataaacatg cttatttctt gtctaattat agttgttagt
    12721 ccaattggtg gagtttttgc taaccaattt gcaattgcta tagaaaaaga taaggagtat
    12781 gcttatccaa tgatcattgg tgcgattgta agtattattg gaaatgtagc ttttgtgcct
    12841 acttatggcg ctgtagcggc tacgatcgta ctcgtggtag tagaattatt agtctttttc
    12901 tttagagtat tttttgtacg aaaagaaata aaaatatcct atttctttgg gaagaacgtt
    12961 atcttatttt ttatagtcaa tcttactttg actacttggg cctattactt tcttccacaa
    13021 ttattctctt cttcattttt agatatggca attaaatcaa taatgatttt cattatttat
    13081 cttccgtttg tttattttct ttttccagat atacgaaatg aaataagaaa gattactcga
    13141 aaataaaata atgttgtaaa aaagtagttc tttgtcaaat cgggctgata aggtcattta
    13201 tgaacaacaa agttagtaag agttactagc ttttaatgca tagctgatta acctgttgat
    13261 tagaaaaatc cacaagcgca tattggtaaa actgcgaaac ccataagcaa atcgtttcaa
    13321 tgttttgata tgtgtgcgaa gattctctaa ttttgttatt agaatacttt ttcgaaaatg
    13381 ctagttaaaa gcctttttca gatatgaaaa aggctttttg agatagtgaa gtttcgaggg
    13441 aacttctttg gtcgtttctc gaagtagcta gctgtttctt gtgataattg ctaagcagct
    13501 tttgataaac tgcttagcaa ttttaagttc ataagagatt gagggtagac gatcaactac
    13561 ttcactttct gttaaatagc gccattgaaa attctgccat ttcttgaatg ttgtgtaatc
    13621 caactcgtca tctgatttta gaagtaattt atacaaaggt ttcaattgtc gatagtactt
    13681 agcttgtgaa gtgtcttttt gattcaactg cttcatcaca cgttttcgaa cacgatcgaa
    13741 aaacttgttc aagtgttgtc caatgtgaaa gcaatcaatg atgatttggg cgtttggaaa
    13801 ggactctttg actaatgaaa aataaggggc attcatattt gtgacaatgt atcgaacccc
    13861 catatgagct tttcttgtaa aacgcatgaa ataagcaatc aaatcttttt ttctccggct
    13921 ggacaaaatg tcaaagagct tccctgtctc tctattcata caagaaaagg agaactttcc
    13981 atgggaaatt gaaagagagc gaaactcgtc gaatagtaaa caatgaggta aatagtctag
    14041 catctgtcgc agcattgccg atagagcaga taatgtccgt tgaaatgtcg ttactgaaag
    14101 atcacaacgt ttcgtaagtg tctttaacgc ttgacgctcg gtcaactctt ctaatatcgt
    14161 gtaatgaact tgtttcgaaa taaaacatga aggaacgaca agatacgtat gagcagtaaa
    14221 atattttccg catgattggt agtggtaatg ttgttttcta agacgcaaat aggtttattt
    14281 attcccactg cgatttaaga taatcattga aatcttcttg ccgtttttca cgagctgctt
    14341 cgcagtttct ttacaaaata gacaatgtct aaaagtgcga actaatgtac cttcgattac
    14401 gatggctttc ttgtccttat aaaaagtttt ttgaaaagaa ttaatttcaa tatttttatc
    14461 tttaatagtg aagtgctctt tgatagaacg agaaatggat atacaaaatc ttcctttcag
    14521 attgatgttt tagacgactc tattttatcg aaatttttgt atgtctgttt tttttacaag
    14581 aaaatctagg atcgacagaa aattctatca gtcctagaaa ttatagagcc atttttttac
    14641 gtcttatcca gatatattgt tctttcattg tttaaaaaat attatttatc actatttaaa
    14701 gaattaagtt cgttctttat ttgagttgta gagatctcag gtgttcttgg taaataaatt
    14761 acttgtgcat ctgttagttg ttcaagttca tcaaattgtc cagcccaatc atcgcccatc
    14821 acgaaataat ctgcatggta ttcttttata tcttttactt tttgttccca attttcttca
    14881 ggaataacta aatcaacgta gcgaatagct tcaagtaact gttttctttt ttcataacta
    14941 aaataacatt ttttttgttt cgcattccaa ttgaattcat ctgtagaaag ggcaacaata
    15001 agataatctc catattgttt tgctcgtttc agtaaattga tatgcccgta gtgaagcaaa
    15061 tcaaaggtac cgtaagtaat tactcttttc atctttcacc tctcgaaatt cattaatata
    15121 cattgttagt ttagcatttt tttgttagaa ttgaaagaac cagtttatcc taaaaaggat
    15181 aagaaatgtt tatgagaaat aggtggatgg aggaaaaatg ttgaatgaaa aatttttaaa
    15241 atatgtgaaa ggttggtatt ttatttattt tggttttatt ctaatgttac gagcaatagg
    15301 cccgtattta ctattaccta ctagatatga tactttactt ttttggcctg ctggaatagc
    15361 gggagttgtc ttgatcatta tagatgcaat tattgcattt agagaaaaaa atcttaaaaa
    15421 atatgattgg ctattaattg cttttattgg cgcgatgcta gtaagcagtt taatgaattt
    15481 aaaatatgga tatagcgaaa atatcaagtt aatcatgtgg caattaatct ttttctttat
    15541 gatttatcag tttggcaaag actacagtag taactttttt ttcaaagtat ttacttggat
    15601 actttcattg gtatggtttt tagctaattt agtatcatta tactggtttg tcattcaata
    15661 tggcataaaa attccaataa aatataagta ttacccagtt cgtttagggt ggttaggcaa
    15721 ccgtttattt ggtgtatata cagatccaaa ttttggtgcg gttatatcat taattgtcat
    15781 cattattgct atatattatt tggtcactta caagcattta aatcttctat ttaaaatatt
    15841 ttttgcaatt aatattttta ttcaattctc gtttattgct ttatcgggct cacgcaccac
    15901 attagtttgt ttattaatag caacttttgt ttttagtttc tttacaagtt atagtagtcg
    15961 ttttcttaaa actaatactt taaaaagatg gctaactgca ttagtagtag gattgctaag
    16021 tataattgcg atgtattatg taacagatat aactgctcaa gcatgggcac atgttccgca
    16081 tattacagaa ataaaaaaag atatatctga ttcatcagga acaactagca gcaataaagt
    16141 tgatttaaaa cgccctgaca ctgatggcgg ggatgtttct aatttaaggt ttggactttg
    16201 gaaaagtgca attgaaatat tcaagagtaa gccgatatcc ggcggttctc caggatacta
    16261 tattgaatac gcacatgata aactaccata tacattaatg ggaaaagata ctttaactgc
    16321 tcataatttt attttcttag ttatggctgc tacaggagga ttaggtctat tgacattgtt
    16381 aaccttctta tttatccaat gctttaaaac tttgcgttat tgtttcacac aaccaaatct
    16441 agtaaaagat aagcactttt ttgctatttt aatgattctt acaatagcag tttcggcatt
    16501 atttatcaca gaactcattt tggttagtac gataggttca tttatttttt ggggatatct
    16561 gggttctatc caaaaaacta tatctaaaga agaaggagta actaattgaa actattttta
    16621 ggtatataaa gtagaaaaga aaactagtta tcccaacttc aacaaatttt atagttttta
    16681 caatataaaa atggtaaact aactttatta agtatgagaa tgcataattg gagtgaattt
    16741 gatggatact atattgatta ctggtggagc tggttttatc ggttcaacgc tagcgaatta
    16801 tttgggaaaa gaaaacaaaa tcgtagtagt cgacgattta tcaatgggaa aaaaagaaaa
    16861 cttggatatg gagaaacaag tgactttcat tgagggggat gtatcagatc ctcaattgat
    16921 ggaaagaatc atgaaacagt atcagtttgc ctatattttt catttagctg ctgtagctag
    16981 tgtggcagat tctgtagaac gtcctctaga aactcatcgt gtaaattttg acagtgcgct
    17041 tttattgctt gaattggtac gaaaatatca atctggttta aaacgattgg tatttagttc
    17101 atcagctgca gtttatggcg atgaaccaac gttgccaaaa aaagaagaat cggtcatccg
    17161 tcctttaaca ccatatgcca tcgataagtt tgcatcagaa caatatgttt tggattactg
    17221 tcatttatat gatgttccga ctagtgctgt ccgatttttt aacgtttatg gtccaaatca
    17281 aaatcctaac tctccatata gcggagttat ttcgattcta gtggaccgat acaaaaaaca
    17341 attggcagga gaaaaaacag aatttacttt atttggtgat ggtagccaat ctagagactt
    17401 cgtttatata gaagatgtta tacaagcact tttacttgtt gctaaggaag aaaaagcttt
    17461 aggacagcaa ttcaatgtcg gtacaggaaa aagcactacg ctacttgaat tgattcattc
    17521 aattgatcaa atcttaggaa cagagctagc gcttaaatat gaagcggagc gttcagggga
    17581 tattcgtgat tctttagcgg atatttcaaa gatccgttct ttaggttatc aacccaaatt
    17641 tgatatattg aacggaatgg aacgttattt aaagacagaa atcgattgaa acaaaaaacc
    17701 agcatcctgc tggttttttt gtctcgactt agctttttaa attaacacct tccatcagtt
    17761 cgtaaatctg atttcttaaa tcagtggccg gcaatttgat gtttttatca gattggatat
    17821 tactttccat gacgattgca tcttttgtgt cttttttcat cgccgctact gcatggaatc
    17881 gattgatatt tccctgagca aggatataat catttccact gtaaacaccg ccgctgtaag
    17941 gatgtttatc accattattt tgccacaatg agaaaagtag ttcttttggt ataatatcgc
    18001 cagacagaac actggtaaag aatttgttca agtcataaac agacatactt atatttcctg
    18061 tgcctaattc atttcggatg tctgtttcag gctcgaccaa tgcttttgaa taatcgtcag
    18121 ttgcagacat ttggtaagat acagcatgct gaggggaatt ttttacattt tcataaaaat
    18181 ctgtatgttc tagatggagt ggccgaatga tttctttttt taaaagagtt tcataaggct
    18241 gattcattag tttgatcaat acccctgtaa gcaatgtata atttaatggt tcataatgat
    18301 acgtatcata accagtaaat ttcaaatgat gaagggcaaa ctgaatgatc tcactatctg
    18361 acataggttt ttttggagaa gcggttcgat gaagaccaga gcgcatgtaa agcatatttt
    18421 tgattgagat ttgctgactt ccaggaatct gaggataaaa ttcagatagc tttgtgtcaa
    18481 aagttatctt gccagattga atttgtttta ggataagtaa agcagtcatc gctttttgga
    18541 tcgaaccgat ttgatagtaa agagaaggag aattcgattc ttttatgcgt agatttgcgt
    18601 acccatatcc tttttctaat atcactcgat tatttttgcg tacataaatc gaaccgatat
    18661 aatctgattt ttgcagtaat tgatcaattt tgctggcaag catctgctca ttagatgtta
    18721 aattggaagc aagttgtgcg ggttccgttt tataaatttg tggttgaggt tgttttgtta
    18781 attgccatcc tgtaatgatg cctgttgaaa agaagctgac agccagcaac aaaactgcga
    18841 gaaaagaaag agagatctgc tgtttttttt gatgttttcc ttttgccata ttaaatcctt
    18901 tctatccatt tttaactatt tttcattgta atcctcaaaa agagcatttg ccacttacaa
    18961 ttttgacata atatttggta aaataattca cggcagtaga aatttcatgt ataataaaca
    19021 gagttacgaa gatgatagta aggaggagtt gttttgtcag gaaacaaaca acacccaagc
    19081 aaagacaaaa aagaagccag taaggcagct acagcagata taatcgacaa gcggagaaat
    19141 ctgcgtaaaa aagaagataa gatcgtgagg aagatcattt tagtgatcgc tcttacatta
    19201 ttgattattg gcggatttct cgggtttacc gtatatcgtt atgtagatag cgggttgaag
    19261 cctttagata aatcggacga ccaattagtc caagtggaaa tcccaaacgg ctcttctaat
    19321 aagcagattg gcgaaatttt ggagaaagac aatattatta aaagtgggat tgttttcaat
    19381 tattatacaa aatttaaaaa cttaactggc tttcaagctg gttattatca gttagcgcct
    19441 aatatgactc ttgatgagat tggcaagcag ttgcaagaag gcggcacatc tgagccaaca
    19501 aaagttgcag atgggaaaat tgccattcca gaaggctacg acatcgatca gattgctgag
    19561 cgggtagcga aggtaacagg taaagacaag aaagaatttt tagatttagt aaatgatgag
    19621 actttcttta acgagttgta tcaaaaattc ccagaattgt tggaaagtgc ctctaaagct
    19681 gagaacacaa aataccgttt ggaaggctac ttattcccag ctacttatga ttactacacg
    19741 agtacttcgc ttaaggatct tgtgattgag atggtgaaca agacaaatac ggtcatgcaa
    19801 aactattatt cagctatcaa acagaaaaat ttgactgtac aagaagtttt aactttggct
    19861 tctctagttg aaaaagaagg cgtaaaagaa aatgatcgta aaaatattgc acaagtcttc
    19921 tttaaccgaa tcaaggcaaa tatgccactt cagtcagata tctctgtttt atatgcatta
    19981 ggagaacata aagaattggt gacgtatgaa gatacagctg tagattcacc atacaatttg
    20041 tataccaata ccggctatgg gcctggtcct tttgacaatc caagcgaaaa ggcaatcaag
    20101 gcagtattag aaccagcaga aaatgactat tattatttcg tagcagatac gagcactgga
    20161 aatgtttatt tcgctaaaac gtatgaagaa catatggaac tagtgcaaaa atatgtaaat
    20221 aattcttaga aaaacagggg ctagtcattg atcattttta gaaaaacatt tacattttaa
    20281 gaaaagcgtg gtaatctttt ttggactata ttcctcaaaa gattaccttt tgcggtattt
    20341 tatcgaaaaa aggagacgtt acacatggta gagaaagttt atccaatgac tctagaaggt
    20401 aaagaaaaat tagaaaaaga attagaagaa ctaaaaacag ttaaacgcgg agaaatcatt
    20461 gaacgaatca aaatcgctcg cggtttcgga gatctttccg aaaactccga atacgaatca
    20521 gctaaagatg aacaagcatt tgttgaagga agaatcacaa caattgaaaa tatgatccgt
    20581 tttgcacaaa tcatcgataa tgacggcgta gatgcagatg aggtatcgat cggtaaaaca
    20641 gttacgttca tcgagttgcc tgatggagat gaagaagagt acacgattgt cggaagtgct
    20701 gaagcagacc cgttttctgg aaagatttcc aatgattctc ctatcgcgca agcattgatt
    20761 ggcaaacgta aaaatgatga agtaaccatt gctacaccag gtggcgatat gttagttaaa
    20821 atcgtcaaag tagaacaagc gtagtctgac tgaaagatct agagagttca tcttactgat
    20881 gacgcactct agatcttttc tctttgagta ttgcaggttt cactgaacga tcttacatct
    20941 aaagtcctat agccaacagt caaaaaaata ggtatgatga aatgagaatg tgtcaggagg
    21001 gagaaaaagc gatgaatagt tcttggcgtt tttttgtggt agtcgaagca ctgctgctgt
    21061 tatttgctgt ttggcaaatc gtaaacaata ctggactttt gcttttagta atattcggta
    21121 ttttcaatat atatctagct atgcgcaaat atcctcgtac taaatttcaa aattttcaat
    21181 tgatcttagg gagcttggtc atttttttta gcttagtcaa cagtcctgct ttatggatga
    21241 tggttgtatt ggccgtttta tttatcgggc tgaaaggtgt agagatttca ggaatagatt
    21301 tgacaaaaaa tgctttttgg cgaaaaaaac agatcatgat ggtccaaaca gagcaattaa
    21361 aaacacataa taatgagcgt aaaagacagc aattatttgg taaccaacgt attggaaacg
    21421 acgtctatga atgggatgat atcaacattg ccatcatttc tggtgatact atcatcgatt
    21481 tgggtaatac acttttgcca aaagatgata atattgtgat tgtaagaaaa gggataggca
    21541 gaacacggat cttagtaccc ctgggagtgg ctattcgttt agagcatgcg acattagtag
    21601 gaaatgtctt gtttgaagaa gagcagtttt ccttaaaaaa cgaacagatc aaaatctgca
    21661 gcagtgatta tgatgaaaat cctcgcagat tgaagatcat taccaatacc ttggttggag
    21721 atgtcgaggt gatacgaata tgatgggaag aatatccaaa gcaatgctag ctgtttattc
    21781 ggggattgcg gctttcctta ttattctatt ttcacttttc acttattttt atgccagtaa
    21841 ccaaagtcat tggtggggag aattgctgcg tgcgcgttta ttatacgttc cgcttatctt
    21901 ccatttgatg gcaatttcat taggtgtagg attgatcgta tttctattgt tatcacttat
    21961 tcaaaaggca aaatacggga aaatcgaaga aaagcttcgt gcactttctt ctggcaatta
    22021 tgaatccaaa cttttgcatc agccgatccc aagcgcgtcg gatgatctgt acatcaaaga
    22081 tattgatatg gaaattgcta aaataaaaga aaaaatgatc gaagtatcca gtgaattaca
    22141 gattatgact agccgtcctc aatatgtaga tggacaaacc aaagaagaga ttttagaatt
    22201 agaaaggcat cgattagctc gtgagcttca tgattcagtt tcacagcaat tatttgcagc
    22261 gatgatgatg atgtcggcat tgacggagca agcagaaaaa agtgagacac cagaaatgtt
    22321 ccgtaagcag ttgaaaatgg tggcagagat catcaacgct tcccagtctg agatgcgtgc
    22381 gctgcttctt catctgcgtc cagtcaattt agaagaaaaa agtttaaaac aaggcattga
    22441 gcagttattg aaggaattgc agaataaaat ccagatttcg ctaaaatggg atgtagaaga
    22501 tgtaaaatta actagttcca ttgaggatca cttgttccga atcgttcaag aattgttatc
    22561 aaacacatta agacatgcta aggctaatga attagaagtc tatttgcata aaatagataa
    22621 caatctttta ttacgtatca ttgacgacgg gacaggattt aatatgaatg aaaccaaaac
    22681 gggaagttat ggattgaaca atatcaaaga acgagtagct ggtattggtg gaacagtaaa
    22741 aatcattagt tttaaagggc aaggtacgag cgtggagatc aaggtccctt tgatgaagga
    22801 ggcatagata aagatgataa aagttttatt agtagatgac catgaaatgg tacgcttagg
    22861 cgtctcttct tatttatcta tccaagaaga cattgaagtg attggagaag ctgaaaatgg
    22921 acgtcaaggc tatgaaaagg cgatggcact tcgaccagat gtcattttaa tggatctagt
    22981 catggaagaa atggatggca tcgaatcaac aaaagcaatc ttaaaagatt gtccaaaagc
    23041 caaaatcatt atcgtcacaa gttttattga tgatgaaaaa gtttatcctg caattgaagc
    23101 aggagcagca ggatatttgt tgaagacgtc taccgcacat gaaatagctg atgctataag
    23161 ggctacgcaa cgcggcgagc gagtattgga acctgaagtg acaacaaaaa tgatggaaaa
    23221 aatgagtcgg cgaaatgatc cagtattgca tgaagaatta acgaatcgag aaaatgaaat
    23281 tttgatgctg atttctgaag ggaaaagtaa tcaggaaatc gcggatgaat tgtttatcac
    23341 actgaaaaca gttaaaacgc atgtgtcgaa tatattagct aagttagagg tagaagaccg
    23401 aactcaagca gctatctatg cgtttaaaca tggtttggtg aaataaaaac gatgataaag
    23461 aggataatca aaagctgaga aaataactat tatttttctt ggctttttta caataatgat
    23521 gcttgctttt gtttttggaa aggctttgtt atactgagaa aagaagatac taaaggagga
    23581 aaatggtgaa acaaaatttt gcaattgtag gccttggacg atttggcgga agcatctgcc
    23641 gtacactgat cgaagctgga caagaagtac tggctattga cagtaatgaa gatcgagtca
    23701 atgaatatat gaatatagcc actcatgctg ttgtggcaaa tgcacaggat gagatgacac
    23761 ttcgttcatt aggtataaga aatttcgatc atgtaattgt cgctattgga gaagatatcc
    23821 aagccagtat cttagttact ctgatggtaa aggaaatggg cgtaccgaat attttagcaa
    23881 aagcccaaaa tgagtaccat gcccgtgtac tagagaagat cggagcagat cgtgtcgtac
    23941 accctgagcg agacatgggg atacggattg cacataactt agtatcaaag aatattctag
    24001 actacttaga gctgtcagat aaatattctt tagcagaaat ccgagtctcc aatcctaaat
    24061 ttttcaacaa aacattagct gaattaaatt tcaggcaacg ttttgactta acagtagtcg
    24121 ccatacgaag agtagatcaa acggttatcg cttctcctgc agcagatgaa tatgttcgcg
    24181 aaaatgataa tctactggtg attggagaga caggcgatgt ggatattcta gacgataaga
    24241 tgaatcaata gaaagaaaag agaaagtagg ttgttgggat ggaattaacg gtaacgccaa
    24301 aagcacaaaa gtggtttgac tcagaaattg atctgccaga aacttacgga atacgttttt
    24361 ttggaaaagt ctacggcaaa acagaggttc atgatggttt ttcgattgga atgtcagttg
    24421 aacagccaga acatcctgta aagaaagtgg aaatggatgg gacacttttt tttatcgagg
    24481 aagcagatga atggttcttt aaaggatatg atttacttgt tgattatgat cccaacctgg
    24541 aggagccaac ctatcacttt aaaaaacaat aaaaaaagag agggtgacac aatttttgtc
    24601 accttctctt ttttcttttg aaa
//
LOCUS       DAFEHS010000034        24396 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFEHS010000034
VERSION     DAFEHS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.034"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            115..696
                     /locus_tag="LP327_001496"
                     /pseudo
     CDS             115..696
                     /locus_tag="LP327_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            700..1341
                     /locus_tag="LP327_001497"
     CDS             700..1341
                     /locus_tag="LP327_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725617.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1338..2255
                     /locus_tag="LP327_001498"
     CDS             1338..2255
                     /locus_tag="LP327_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL1725618.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2252..2923
                     /locus_tag="LP327_001499"
     CDS             2252..2923
                     /locus_tag="LP327_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725619.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3464..3952)
                     /locus_tag="LP327_001500"
     CDS             complement(3464..3952)
                     /locus_tag="LP327_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBL1725620.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4153..4419)
                     /locus_tag="LP327_001501"
     CDS             complement(4153..4419)
                     /locus_tag="LP327_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL1725621.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4419..4682)
                     /locus_tag="LP327_001502"
     CDS             complement(4419..4682)
                     /locus_tag="LP327_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL1725622.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5158..5343
                     /locus_tag="LP327_001503"
     CDS             5158..5343
                     /locus_tag="LP327_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL1725623.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5485..7308)
                     /locus_tag="LP327_001504"
     CDS             complement(5485..7308)
                     /locus_tag="LP327_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBL1725624.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7906..10611)
                     /locus_tag="LP327_001505"
     CDS             complement(7906..10611)
                     /locus_tag="LP327_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBL1725625.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10917..11864)
                     /locus_tag="LP327_001506"
     CDS             complement(10917..11864)
                     /locus_tag="LP327_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBL1725626.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            12054..12323
                     /gene="rpsN"
                     /locus_tag="LP327_001507"
     CDS             12054..12323
                     /gene="rpsN"
                     /locus_tag="LP327_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL1725627.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12499..12861)
                     /locus_tag="LP327_001508"
     CDS             complement(12499..12861)
                     /locus_tag="LP327_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL1725628.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12872..13993)
                     /locus_tag="LP327_001509"
     CDS             complement(12872..13993)
                     /locus_tag="LP327_001509"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBL1725629.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(14009..14359)
                     /locus_tag="LP327_001510"
     CDS             complement(14009..14359)
                     /locus_tag="LP327_001510"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBL1725630.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14489..16000)
                     /locus_tag="LP327_001511"
     CDS             complement(14489..16000)
                     /locus_tag="LP327_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL1725631.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16322..17674)
                     /locus_tag="LP327_001512"
     CDS             complement(16322..17674)
                     /locus_tag="LP327_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBL1725632.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17827..18903)
                     /locus_tag="LP327_001513"
     CDS             complement(17827..18903)
                     /locus_tag="LP327_001513"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBL1725633.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            19099..20844
                     /locus_tag="LP327_001514"
     CDS             19099..20844
                     /locus_tag="LP327_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBL1725634.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21124..21561)
                     /locus_tag="LP327_001515"
     CDS             complement(21124..21561)
                     /locus_tag="LP327_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBL1725635.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21545..22918)
                     /locus_tag="LP327_001516"
     CDS             complement(21545..22918)
                     /locus_tag="LP327_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725636.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23240..23728)
                     /locus_tag="LP327_001517"
     CDS             complement(23240..23728)
                     /locus_tag="LP327_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1725637.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23750..24352)
                     /locus_tag="LP327_001518"
     CDS             complement(23750..24352)
                     /locus_tag="LP327_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL1725638.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6997 a         5196 c         4511 g         7692 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaactagcaa gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca
      181 gaaaatattt tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc
      241 ctactacaag ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa tacagcaatc
      301 acgattacac cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta
      361 gatacgttgt tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg
      421 ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca ttttgaataa acatgttttt
      481 tacgtaaaag aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcgggctg
      541 aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca
      601 ttagtggata tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca
      661 gagtcagcta ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag
      721 cacggttcag aaattctctc aaaaggtttt gaacatcttt atatttcagg acttgctttg
      781 atcattggta tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct
      841 gccacgattg tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg
      901 gcattgatga tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt
      961 tattcattat tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat
     1021 tatcgggatg tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa
     1081 ttgcccattg ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt
     1141 gcatgggcta cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt
     1201 ggactgaata attaccaacc agatctcatt ttggcaggta cgatccctgt aacaatttta
     1261 gcactattgg ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt
     1321 agagaggaga atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc
     1381 ttatacttgc gagttgttcc ttccctggtc ttgccagcaa tacagatgaa gacaccattt
     1441 cgatcacagg tggaatcact tcagaagcac agatcttagc aagtctagtt gccggaatgg
     1501 ttgaacatta tacagataaa aataccgcaa tcatcaacaa tctggctact acaactatca
     1561 accatcaagc gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag
     1621 atttaacgac tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg
     1681 tgaaagatga atttgagaaa agatacggtc aaacgtggtt cccgtcctat ggttttgaaa
     1741 atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta tctaaagtca
     1801 gcgatctgaa aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc
     1861 gtaaaggaga cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat
     1921 tacctatgca gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc
     1981 ttggctactc tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc
     2041 gtcaattctt cccaccatat gatgctgctc cagttgtaga caacaagata ttagctgata
     2101 cgcctcattt aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc
     2161 aggaattgaa ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt
     2221 tcttacaaga aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt
     2281 tcaacaattt ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg
     2341 tcacttcctg atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg
     2401 gatcatgatt tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat
     2461 ccagacgatc ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt
     2521 caatgtcgtg attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta
     2581 taccgggatg atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac
     2641 tgctagacaa cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg
     2701 cataagaaac gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc
     2761 tggtggtctt ggagatatca ttatccgagg aaccaatgca acagagggca catcgattat
     2821 tttagcaggt gcattgccaa ctgcattaat ggcgatcatc acggactggc ttttaggtat
     2881 tttggaaaga cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca
     2941 aaagcttctg cgaccatttg ttggtcgcaa aggctttttt agttctgtca tttttaaggc
     3001 aataatccac tctgattctt ccctccttat gggcaaagag ttgcttttct tagctattta
     3061 tccattccgt atactccaac ttcatactgc caacgggttc atatctgcat ccaccgacta
     3121 agtttttttc ttatccgcta aggcattttg ccaatccatt ttcccatcac tcgtcatgga
     3181 attcatacca catgatctct aaatactcgt ctgcttgttc tttgaattgg gcatcaattc
     3241 ttacatcctt tggcgtggct ttcatttaag ccttttccat cagtcctcga ttgttcgatg
     3301 ccttcagctg catcatcaaa actggtgact gccgtatcgg taacttcgat catcttttat
     3361 acaatcggga acttgccatc ctcatccttg gctaaaaaca tatctagcca ttccagcttc
     3421 tcgccatggg aggtttgcgg ttcctaaaac cggtacacga ttcttatgga ctgcatccgt
     3481 cgcaactaga cttggcgata cgatgatcct tcaccagaag agcacccccg taaaccaatt
     3541 gatcgatata ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc cctcgtgatg
     3601 cattgccgtt gatactgcta ttcatggccg aaagcgccac gactttaact tcttccgttt
     3661 gagtgacatt catgttcggc aatgtcgctt tggatacccg ctctgctaaa ggaacgacac
     3721 tggtattgta tttcgtatct gtatcttttc caaaagacca tgccaacgaa tcctctggga
     3781 accaataaga cgactccggt tgattgtcca ttgctttttt caacatactt ttggctgttt
     3841 ctcgataaac gacatttcca attgtcttcg cctcactttg gcgataatcg atggtcgttg
     3901 ccactgccaa cactaacaac gatgctccaa ttttactgat tttccttgtc acacttcttc
     3961 ctccttgttt tacataaacc cgtcaacatt ccattcctaa actcaaacaa tgacccttca
     4021 aaggtggaga tataggaaca aattaaagaa atcatgcaat aaatccaaga aggttggttt
     4081 tagaacaaaa aaagagtctc ctttttgggg agactctttg ttaactatct aaaaaggttg
     4141 gctcttctca cttcactcat aatgtgtcca ggcagaataa atttctacaa ctttattttc
     4201 ctcatcaact ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt atctgccttc
     4261 atgtgtaggt ctcagctttt caaaagactg cgtgggcata tatggatctt ccttcaatgt
     4321 ctgaatcact tcttcaaatt gtttctttaa attcgtttgc ttgatttttc ttaaatccac
     4381 tttcgcagag tttttgattg cgactgtata gttactcatc aaagagcttc ccaatcaata
     4441 tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt atctaaagta
     4501 cctgtttgct caagaaaaag ggtttcttga atcgctctcc agtcttccag cccaatcaat
     4561 acagcattgt ttccactacg atcactaata atctcgattt cagtatgatt ttcatttacc
     4621 tctttcaata actgataaaa atttttacga gcagtgctag gattcatcgt tgtattagcc
     4681 attgtctctc ctcctttttc tgatataatt atagcgtact tgattaagta cgtcaaaaaa
     4741 atacaataaa gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc
     4801 cctttttagg gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc
     4861 caaacgtaaa gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg
     4921 tgagtctatg caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat
     4981 atcttgaata acctgaggat acgcacgaaa gatttgtaac ggttattcaa aactgatttt
     5041 ggccgtcaag tcttctactg cctcaaaaat gattgcctct ttcgtctcct gtcattcatg
     5101 ttcactatcg atttgatact tgcttagacc gttgggtttg tgaatcacca attaaaaatg
     5161 gcactgagtt atggtaacaa aaaatggtca cttgacgagg ctgagattcc tacttatgaa
     5221 gacacatggc acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca
     5281 ttatttggca aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc
     5341 taaataggaa gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg
     5401 cacctttagc tgagaataag acaaaacaga ccctctagct tcaattaaga gctagagggt
     5461 ctgtttttga ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac
     5521 gatattttca cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat
     5581 attctgccag cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg
     5641 tttcgctgca tttttactta cgatatctac ataacggata attggatttt caatcgagcg
     5701 ataagaagag tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc
     5761 gatttctttt tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc
     5821 accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa
     5881 tacaagaacg gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg
     5941 ttctgctact tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat
     6001 aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg caatgattgc
     6061 atatgagatg aacgcacccg taatattggc aatcgatttg cttaaccatc tttttccttc
     6121 ttttttccat ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat
     6181 agagtataac ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga
     6241 acctagcgct agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata
     6301 catgtgaggc atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa
     6361 accggtgtta gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa
     6421 aataccatgt ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg
     6481 aacgatccca taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa
     6541 tgccatcatc gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac
     6601 agcattacta atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa
     6661 aatcaaagcg atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg
     6721 cacagcacct gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat
     6781 aaatgtgtac accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag
     6841 catcatcagt agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg
     6901 caccgctgat gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta
     6961 gtcgatcaat aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct
     7021 gacgacatat gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt
     7081 taatgaaatc aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc
     7141 agcagacaaa gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa
     7201 cgcatcagac gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc
     7261 agcagatttt aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc
     7321 tttacacaca attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat
     7381 agtacgctca ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga
     7441 aaaatatcaa gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg
     7501 gctttttctt tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat
     7561 cctttttgat atcttaactt ttggaagaga gatgtaaggc tgctttctac ccatcattct
     7621 tactactcga agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg
     7681 catctgcggt aataagctca accaaattaa aaacataaga agctgttttt atttggtcgt
     7741 tcttattact tgatgcagag cagctttttt acaacctctt ggtacacggt gttcaatcag
     7801 ctgaccctcg gtattagctc aaaaaaacgg caaaatatga ggaactattt ttccattttt
     7861 ttctctaata ctcaggtcaa aacactgatt tcacaacctc tttttttaat gaaaaatatg
     7921 gctctcatgc gccgcttctt tgaatttcgt cattttttct tcgatgaacg gatagattgc
     7981 cgttcctacg attccaaaga caacaaacaa accgatcatg atccagattg ctttcgtagc
     8041 atttggccaa taaattcctc ctgcagattc ccgtaacaag tttaccgcat gagtaaatgg
     8101 caagaacgga ttgatcattt ggaagaattt ccctgagact tggatcggat agttcccgcc
     8161 tccgccagag atcgacagta ctaagatgat aatagcgatc cctttaccga cattatcgaa
     8221 taatgcagcc aagacataga cgatcatcat gaacgctaag gcgatcaaga cagcaaatga
     8281 cacgctgtag accggatttt ggacatctac tcctagtaag aaaatattcc ctaatgtaac
     8341 gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa cgaattgttc
     8401 tcgtttcgta aaattgtttc ggtctttctt tcctaaatag tattctgtcg tagcaacact
     8461 tgaaagaaga acagcgccga cccacagaca tagtgcagta taaaacgggg tgctagctga
     8521 accgttattt gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt gtgtaaagaa
     8581 gtcactttct tttgttgcgt ctagtttcaa taatttgatg acttcgccta gatcgacttc
     8641 ttgttcgcct tttcggatcg cgtctgctgc tttttggata ccagctttga tatttggcca
     8701 gtcattgttg atcaagtccg cagcagcgtt cacagctgtt tcgacttctg gcattttttc
     8761 atttactgtt gtcaatgtac cggtgatttc attttttagt gctggccaat cattttggat
     8821 aaaatcagcg gccaatccta atttttgctg aatgatcggt aattcattgt tgtataggtc
     8881 tgccccttca ttgatgccat tgacgatggt atccatattt ccattcagca ttgtattggc
     8941 atcatggatt tcttgtccaa ttgcaggcat ttctttttga tatttttcta aaattgccac
     9001 tgcattcgtt acagttgctt tcgttgagct gagaagcgaa tcaaagtcga tctggtccgc
     9061 tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg acttggtcta gtagagaagt
     9121 gacagtggaa ctgatttgac ctgtattgat accagcaatg atgccattga tattattaac
     9181 aacggcatca atttgatcta gcaattcatc aatagccttc gtgtcttcat tttgaacagc
     9241 tgtgttcaaa gcagataggc ggttttgcag attgcttagc aagggtttta attgatttag
     9301 ctgatcaata attggctgta atttcgtatt tccagcactt gtttgcaatt tagtcaggaa
     9361 ctcgaccagt ttatcgattg cattttgttg ttgagatatt ccgtcaataa agccgttcaa
     9421 tacattgctg atgtttttct tttcatcatc agtcagctta ccatcttcaa tagctgttct
     9481 tacagtatct attaacgaac ctgtcgttgt tgccacttgt ccaattgact tcaatgtaac
     9541 atctacactg tttgtgatgc ttggtatcgc atctttcagc tgagaggcac ctgattttgt
     9601 ttggtttgct agatcacttg cctgattttc taaatttttc acatctggca agatatcttg
     9661 tacttgttgg atgatcgtca agccttgttt tgcttcatcg attccttggt tcattgtctc
     9721 ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat tttggatctc
     9781 tgggattttt tcttgcaaat caaggatgat ttttgcttga tctttcaact caggcatttt
     9841 gtcattcaat gcaacgactt tttcacccat ctcatctact ttagggatgt aatccacgaa
     9901 ttcatttgct ttattcagtt tttccttgat ttcaggtagt tgactttgta attcgaggac
     9961 ttgttttgtg tagccgtcga tcgtgtccaa attttcatct gttgataaaa tcatatcttt
    10021 aactttattg atacttacta ggttcgtatc gatgtcgtaa cctatctcat tgaatacttt
    10081 caataaagtc gaactggctg ttttgataaa ttcatttgtg atttgcgatt ggatcgaact
    10141 tgctccttta tcggtgattt tcggtgcaat cgcattgatt ttttcattta ccgtatattc
    10201 gatttttggt ttcttgatat caccactagt aaaactcagc agatcttctg agaaatcctt
    10261 tggcaagtaa atacctgcgt aatactttcc ggaacggact ccgtcttcta attgttcttt
    10321 tgaatcaacg aattgccatc ctaattgttt atttttatga agcgattcga tgacttgctc
    10381 cccaatggcc acttctttac cttgaaactc tgctggcttg tctgcactgt aaacagcaat
    10441 tggcagctct ccagtatttc catatggatc ccataatgct ttgatgttaa accacgcata
    10501 tagcgaaggg atgatcatga tcgcgatgat cagaagtgtt gcaatcgggt ttttgaaaat
    10561 ccgtttccag tcaagcaaat ataatcgaag cgtatttttg attgactgca tagttctccc
    10621 ccttcttctt ttttcagctg aattacttta ccatgtattt catacttttt tcaatcggct
    10681 tttttcaaaa aagtcgaatt tttggacata tcacggcaat tgtctcattg taatctctta
    10741 catattgaga aataattaag gattacagcc tttactttat catgaaataa aagggaaaga
    10801 taattttatg ccaaagtttt tatctattat acgtatacgc aaaaaagcta aaacattttt
    10861 tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt tttattcctg tatttcttat
    10921 ttttcgaatg acaggatgat tttcccagca ggaggatgtc cttcaactag ctgatgtgct
    10981 tggatgacat gcttaagtga agcagacaga ttcattgaaa taaaaacatg aaaggctcct
    11041 ttttggtagg ctttgattat gccagcaaag gcttctgcat ctagatattc ctttcttgga
    11101 acaaacgatt cataaaagcc ttcctttttt tgccgcaaat ctagatctgg gaggtcattt
    11161 aatgcaacat acctgccgcc tggtttcatg atctggatgc cggtttctcc tttgatactt
    11221 cctttcgtcg catcgattac aaggtctgcc tgatcagcaa attgcagacc tggatttgtt
    11281 ttatcataag cagcaaatgc tgaagctcct aattttttta ccttttcctc atttttactg
    11341 gatgcactgg ttaagattcg gattcccttt tcatggagaa gctggatcag acttgacccg
    11401 accgcacctg atgctcctag tatcattaca gtatctgtcg gctggatttc cgtcaaatgg
    11461 ttgattagat tataagccgt aattcctggc gtgaccattc cggctgcttc ttcccaactc
    11521 attttatctg gaatttttgc tacttttgct gatggcaaca cgacttcttc tccgtaagtc
    11581 ccgccgactg catggacagc tactcgatca cctacatgaa cagttgtgac atctgaacca
    11641 atctctgtca caataccagc gccatcatta cctggtacat aagggaattt caatgttcgt
    11701 atctctttca tttctcctaa acgcaaggca acatcataag gattcacact gaatgctttt
    11761 atttctacac gaacatgtcc agaaccgact tctctgggat gagcatcgat ttcttcaaaa
    11821 acatcttgtg cttctccgta accgttaatt gcaaatcttc gcatggtcac catctccctt
    11881 tttctttaag tttacgttta tgtctttatt ttgacaaaaa aaatgtcttt tcgttttggc
    11941 gcatgattca aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa
    12001 aagcgtaaag attacgattt acgctaataa aaaataattg gaggaatttg ttgatggcta
    12061 aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt
    12121 tacgccagga attaaaagcg caaaaagatt acgaaggctt gcgtaaactt cctttagatg
    12181 ctcgtccaga acgattgaaa aatcgtgatt taatcgatgg acgccctcgc ggatacatgc
    12241 gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc acataaaggt ttgattccag
    12301 gagtaaaaaa agccagctgg taatccaagt ctggctgtaa tgtaaaggag gagggcgcat
    12361 ggaccactcc ttggatcatc aaaaaagata cgttcgcttc ttctagcaga aatgctggta
    12421 ctagaagcag acgtatcttt ttttacgcac agctggtcct tttttatatg taaaggctgc
    12481 atggttactt cgctatattt atttagtaac aggagtcagt gcttcgatac ccacactaat
    12541 tttcaatgct cggtcaactt ctgccataat gggtcgatcc aaatggcaga ccttttcttt
    12601 caatcttgat ttatcgatcg ttctgatctg ctccaacagg ataacggagt ctctttctat
    12661 tcctgttcca gtagctttga tcccaatgtg agtcggaagc ttaggtttag ccatttttgc
    12721 cgttatggcc gcaacaatga tcgtcgggct aaaatggtta cctagattat tttgaataac
    12781 caaaactggg cgtgttccgc cttgctccga tcccacgact ggagaaagat cagcaaaata
    12841 aatatctcct ctttttacca taggatcctc cttattcttg atattctctt ggtatccgct
    12901 gtcctaatcc gcaagccact tcatagtgga tcgtacctaa ttgatctgct atatcctgca
    12961 tagtgatttc tttgtctgca tttttgccga taagcgtcac ttttgttccg acaggaaatt
    13021 cttgaggcaa tcggatcatc agttgatcca tacatacacg cccaatcgtt tcacagtagt
    13081 tcccttctac caatagagaa aaaccctgca tctttcgtgg ccagccatct gcataaccga
    13141 tcggtatcgt tccgatccac tctgcttcag gagtgatata tgtttctcca tagccgattc
    13201 cttcacctgc tgggagtttt ttgacttgaa tcaattcaga aaccagctca agtgctggct
    13261 gcaatggata aacttccggt agtgcgtgcc cagagggatt aagtccgtac attgctactc
    13321 cataacgaat catattgccg attgtttcat catgccataa agccgttgca ctattgctga
    13381 catgtacata tcgaggaagt tcgggaagtt ttttcagcac ttctataaag cgttcttttt
    13441 gcagattcca atatgtatca tctgcctgat ctgctgtaga aaaatgggta aataaacctt
    13501 cccattctaa tacacgactt tctttgatga atgcagctgc ctcttttact tcttcaggtg
    13561 tacaaaatcc gatacgaccc atcccagtgt ctgcttttag atgaatcgaa agaggcgtct
    13621 ctagcttact tttctctaaa acagcagctg ctgcttgcaa ccattcccgt gtacctgcag
    13681 ttactgataa atcatgagca acagctacag tcagtgcgtc gtaaggaacc atattcaaga
    13741 taaggatggg ctgagtaaat ccagcttctc tcaactcgac tccttcatct aaatttgaaa
    13801 cacaaaagcc cgaagctcca cctgcaactg ctgcctcagc tgtttcaata gctccatgac
    13861 cgtatccgtt cgcttttact actgcaaaca attcttttcc ttgcggaagt cttgcaactt
    13921 catttgctac attttcagtt atagcttttt tatgtatcac tgctttcgtt gggcggtgcc
    13981 aagcgacaac catcgttttt ccttctttct agtcttccaa aataatttgt gcaaaagctg
    14041 tttcatttgt atgagtaata ctgacccata cattgccttc atgaggtgaa cgcgtcacaa
    14101 caggctgacc attttcattt tttaaaatct cgacatcttg aaaggtaact tttccgatcc
    14161 cagttcccca agctttagag aaagcctctt tgcaagcgta acgcccgcct aaaaattcta
    14221 cttggcgatg aaaaggcaag gactggaata gtttcatttc atcagaggtc aagattcgtg
    14281 caatgaattt tggtttttct tcgatcagcc gagtgatcct aggaagttcg actgcatcga
    14341 tcccaattcc ttttatcata tgcttttctc ttttcaataa atttagaaca ctctcattct
    14401 accaaattat aaccattttg ccacaaataa gtccacaaat aacaaaaatg aaagtttctc
    14461 ttaaaaaaga gaaactttca ttttatgttt aatcgttatt tgaacggatt acaaatccgc
    14521 gttttttatc attgtgacgt ttaccgccat ctttttggct gttattattt ttgctataac
    14581 cgccttttga tttgtttcca cgataattac cgccgtcacg acgattacgg ttgtttccgc
    14641 caccacgttt gccattttta ttgtatcctt ttttctgcat tggcaatggg cgttctggtg
    14701 tgatcttcac tggcactgca tcagctggat cttttgctgt cgtttttagc aaaagtgcaa
    14761 ctaaatcttg tgcagaatat tcttctaata atttatctgc tgtttgcagg tatttatcta
    14821 gaccattttc tgcaagtttt gtttcgattt gttcaactgc agcacctaat tgacctttga
    14881 atgcttcttt ttcggttggt ggacgtaaag tcgtcatccg ttttttcgtc aagttttcaa
    14941 tcacatgcaa gtagtccatt tcatttggtg taacgaaagt cacagacata ccgccttttc
    15001 cggcacggcc tgtacgtccg atacggtgaa cgtagctttc tggatcttga gggatatcat
    15061 agttgtacac atgagtgaca cctgaaatat ctaatccacg tgcagcaacg tctgtcgcta
    15121 caaggatatc taaatgaccg ctcttaaatg aacgcaatac gctcattcgt ttttgttgag
    15181 acaaatcgcc atggattcct tctgcacggt aaccgcgtgc ttctaatccg cgtgccaatt
    15241 cgtctacacg gcgttttgta cgtccgaaga cgattgttaa ttcaggtgtt tgaacatcaa
    15301 acaagcgagt catgatatcg aatttttcgt attcttttgc gcggacatag tattgatcga
    15361 tcaagtcagc tgtcatttct ttcgctttga tcttcacatg atgtggattt ttcatgaatt
    15421 taacgccaat attcttgata gctggcggca tcgttgctga gaaaagtaat gtttgtcttt
    15481 gatcaggaac ttgtgaaatg attttttcga tatcttccaa gaagcccata ttcaacattt
    15541 catctgcttc atccaaaact aatgtttgaa cggttccaag tttcaatgtg tggcggttaa
    15601 tgtggtcaag catacgtcct ggtgtaccca caacgatatg tggacgatct ttcaagccac
    15661 ggatctgacg gccaatatct gctccgccgt aaacggcttg tacacgtact tttttgtcgc
    15721 gccctaaacg atataattct tcttgtgttt ggatagctaa ttcacgtgtt ggtgcgataa
    15781 ctaaaccttg taattcgtga cgatctggat cgattttttc taacattggt aatccaaatg
    15841 cagctgtttt ccctgtacct gtctgtgctt gtccaataac gtcttttcca gctaatgcta
    15901 gtggaatcgt ttctgcttgg ataggcgttg cttcttcaaa accagcgcgt tcgactgatt
    15961 tcaacaattc tggtgataat tctagttctt taaatttcaa atgaatcctc ctatatggtt
    16021 gttaactgag gatgtagctt ttttgctacc ccgtcgattt gttcggtcaa agtgtattgt
    16081 ttctttttga cagtggccac gcacgttttt atccattctt agcccctgtg gcaccaccga
    16141 aaaatcactt tgcgaagttt aggcattaga aaagccaaac gggtcgcgtt cagctttatc
    16201 attattatga catctggctt caacaagttg tttctatggc aaaaccagtc caatttctct
    16261 tcatctttcg gcaataggtt attctaacat agtttttcgt tttctgcaag tttacagatt
    16321 tttatttcat ttgctgaagt tgttctacca cttcaaataa gcccatgctg ttgctgcctt
    16381 ttagcataat actatctgtt gctgcaagat tttgtttgat agcatcaatc aatttattct
    16441 tttctttttc aaaatagaac acaggtagtt gggggtattt ttggttcaat tcttctttta
    16501 gtgcatgcat ttgttcccca taaaggaaaa tcatggcgaa tttctcagga tcgatatgtt
    16561 cactcatttg cgcatgcata tttaaagact ctttccctaa ttccagcata tctgctaaca
    16621 cagcgattct gcgtcctttt gttggcagat ttttaaaagt atctaatacc agtcccatgg
    16681 ctgttggatt tgcattgtag acatcgctca aaatagctgc accatttcct gccgttagcc
    16741 attcggttct gttttgagtg atagaaacat gggcgagccc ttggcgtatc tcaggtatgg
    16801 acagtccaaa gaagcgcccg atcgtatacg caatcaaagc attcgttaca ttataacttc
    16861 caggcagcgg gatcgtaaat gtttctcctt ctactacaaa atcggtttgt gcttttcctt
    16921 ttgctgtcac ttccgcatga acatcgccat ttccaatacc gaatgttgtg actgtttgta
    16981 ctaatgttgc cgttaaaggt gtcagcaacg gttcatctgc gggcacaacg agtaatccgt
    17041 ctgatacaag tccgtcagtg atttccattt tggctttagc gattccttct cgcgacccaa
    17101 gattttctat gtgtgcttca ccgatcattg tgattgctgc agcttttggt tgagccataa
    17161 gtgaaagttc cgtgatttca ttggcgtgat ccatgcccat ttccaaaacg atcttttctg
    17221 tatcttgggg catgtgcaaa atcgtgtaag gaagaccaat attattgttg taatttcctt
    17281 gcgtcttata tgttttgtat ttttcagaca agacagacgc ggttagatct ttcgtggtgg
    17341 ttttaccatt gcttcctgta attgcaacga cttctgcgcc tactttttcc aagtaatagg
    17401 ttgccaattg ctgcatggct ttcaaaggat ctgctacagg aataactgga aaattttttg
    17461 gagcttgttc aacaggcaag ctccaaaatg aagcagatgc tccgtttgtg atcgcttgct
    17521 ggataaattc atgtccatct cgcgtaccag caagaggaac gaataaactt ccttcggtta
    17581 tcttccggct gtcaaattcg acattggtaa tctttgtcag tgcagagcat tcgctgccca
    17641 cggcttgtgc aatttcttgt attgttagat tcattgagat ctctcctaaa aaaggttgtg
    17701 aaaaaagcgt ttggcctaaa cggcgggttc gcatgactct cacttccaag taaatttctc
    17761 tattcgtatt tagaagctag aatgaacgcg gttacgcaat cactccagcc tcttctttat
    17821 ttctctttat tcattttttt caaaaaaaga ttgacgctga tggtatcgat tcattcctaa
    17881 ctggatcagt tcttcaatca aatcaccgta tttcaagccc atattttccc ataagagtgg
    17941 gtacatactg aactccgtaa atcctggcat agagtttaat tcattcagga ataattcatt
    18001 tttatttgtc aaaaagaaat cgcaccggct caatccgctt ccacctaaca tcgtgtaagc
    18061 taacttcgcg tactcttgcg ctttttgata aacttcttct ggcacttcgg ctggaatctg
    18121 catttcgatt ttattattga tatattttgc ttcataatca tagaatgcta cgtcttttac
    18181 gacttcacca ggcaaagtcg tccgaacgtc ttcatttcct aatacagcaa cttcgatttc
    18241 gcgcgcttca attccttgtt caacgattgc tcgagaatca tactgatagg ctgttgctaa
    18301 agcattttgc agctcttctc ggttttctgc ttttgtaatg ccgacactag aacccatatt
    18361 cgccggtttg acaaacatcg gataaagcaa agaaccttca cattgatcaa atactttttt
    18421 aggattttct ttccattgat tcttaagtac tggtacataa ggaacttgcg gcacaccagc
    18481 agcttgtaaa atatacttgg tcatgatttt atccatggca catgcactgg tcaatacgcc
    18541 tgcgccgaca taaggcatat tcaatgtctc taagaagcct tggatcgttc catcttcccc
    18601 gtttggccca tgtaaaactg gaaaaacgat ggctccttct tctttgattt cgcccggatt
    18661 gatcactttt cctgtaaagc cttcctctgt ctgtccactt gggtcccatg aaagatgcaa
    18721 gacatctttg ctggcaggtt tttctgttag taatggacct ttgacccatt gtccttcttt
    18781 tgtaataaat acgagttgaa cttggtaata attataataa atggcattta aaactgaaaa
    18841 tgcggaaaga atggacactt catgctctgc gctgcgtccg ccatatagta aagtaatctt
    18901 caaaagattg tcctcctgta ttctaaatcg ttcagtgatt tactctcata ttttagcaca
    18961 aaacaaaaat aaacgaataa cttatttata aaaaaacgag ccagcttcta aaagaacgag
    19021 cgtcatttat tttttgtcag ctagattatt tgtcccgttt tttataaaaa gttatgataa
    19081 agaaaaggag ggatgatgat ggcgcaaaca aatccacgca gccgcttggc agaaatcatt
    19141 cgagtgttca tcaattacaa tgtagtgcca aactttgtac agcaaaaaaa tcccgaacag
    19201 gtaaaaaaag cctttgaaga actaggcccc accttcatca agataggaca gatgctttct
    19261 gttcgagaag atttgctttc ttctgccttc actcaaacat tcaaaacatt acaagatagt
    19321 gtacctagcg acacattttc aacggtcaaa aagacgatcg aaacagaact ttctctttca
    19381 ttatcagata tattcgatga tttttcaaaa tcgccttttg cctctgcttc tatggggcag
    19441 gctcatcgtg ccaagctgaa gaacggtgac gcggtagtcg ttaagatcca acaccctaac
    19501 atcgccgaag agatccgact tgatcttcaa ctatttgaac gagcgatccc tttgatcaaa
    19561 tacattcctg aaactagcgt agttgatcta aaaggggtac tccaagaagt aaaacgttcc
    19621 ttgatcaatg aaatggactt cttaaaagaa agtcaaaatg gcgaacaatt ctatcaaaag
    19681 aacaatggct ggaaagagat ccgcagcccc aagatttatg acgctttttg ttcgaaaaaa
    19741 gtgatcgtga tggaagaaat gtctggtaaa aacttgaatc atttaatgaa cgccgaaaac
    19801 aaaactgaaa cttttatcac tggaattcaa aacaagcagc tgaaacaaga agttgcaaag
    19861 cttttagttg agaactttat gaaacaggtg tttgatgatg gttttttcca tgctgatcct
    19921 catccaggga atctgctatt ccatgtacta acaaaagaag aacagacaca agctagccga
    19981 aaaactgaaa cagtccatga aaaagagttc ggttcctttg cttttcgagc ctccacttct
    20041 gcagaagacc ctgttgcgcc atataccatc aactacatcg attttggtat gatggggcat
    20101 ctttctgcag gacttaggca aaaactcaca caagccgttt tagcgctgta tacgaaagat
    20161 gcttatcgaa tcgaaaaagc ggtccttcga ctttgtcagc aagaaggctc gtttgatgaa
    20221 agtcggttcc accaagaact gacaagtttt cttgaacagt attatgattc tcctattgat
    20281 gaaatcaact tgcaagaagt attcacacat gtcgtgacga tttgtcatca aaataatctg
    20341 cagttcgatc gtgatatcac gcttttgctg aaagcatttg ggacattaga aggggtgatc
    20401 cgcgtcctcg atcctgaagt ttccctcatg gaagtcgctt cgccttttgc tcaacattat
    20461 tttctcactc atctggatgt agaagataca ttgaaacaaa gcggattaga cttattggaa
    20521 gggatgaagg ctgcgcctaa gatccctcaa cagctccacc atctattaga aatgtggact
    20581 tcgggacaag gaaaagtcaa tttggaattg aaaaagcaag acaaattatt gagcaggatc
    20641 gaaagtatga tcaatcggct tgttttcggt atgattttag ctgcattgat cgtagggtcc
    20701 tccctgcttg tccaagcagc acctgtagaa aacgcagagg tcgtttcctt attagggata
    20761 tttacgtatg ctattgccgc attcgtcatt atttttttgg caatcgatgc attgatccaa
    20821 atgtataaga agaggaaaaa gtagaaaaag gttgtgacag aaagaatcag cttcaagaaa
    20881 taagaagggg ttttacataa attgtttctc aaatttttgt gaaaattcgg cttatttttc
    20941 gaagaggcta cttctgaaac accgagaata aaaaatgtga aaaagctgtt ctgcacccag
    21001 taataagaac aatcaaaaag ggaacatgga acacatttat cctgtttccc tgttcccttt
    21061 ttgatgtatt cgagcataaa taaaatacct tcgttaccta ctatcttctg tacttggtaa
    21121 accttagtca attgcctttt ttaacgcttg acgaaaagac atatgatctt tctccgtaaa
    21181 gggttttgga ccctttgtcc ttatgccagc ttttcttaac tttccactat gataacgttg
    21241 ttctagaagc gtgccgatat tcgcttcgtc aaattctttt ccagagtgat ggaacactcg
    21301 cacacccttc ggcaatacaa gtgaagctaa accataatct tgcgtgatca aaaagtcatt
    21361 cttcttaacc ataccgacga ttttataatc cgcactatca gctccgcggt ctacgtagac
    21421 gatttggaca tgattagcgt attctttggt cgtataatgg tccacactgg tcacgataac
    21481 cacgtcaaga ccataggatt ctcctaattt tatgacatct tcttttactg gagaaccatc
    21541 tccatcaatg aatagcctca ttctttttcg ccttcttcca taaatgataa acagttacta
    21601 atccgctcag taaaagacca acattgatcc attttgccaa gataagcagt tgtacacttt
    21661 ctatcggcac tgattcatgg ttcagcgtga tcaataatag atggattggc tctcccattt
    21721 cttttgctaa aacaaacaaa agcgtcatca ataaaaactg gtggatcaca ctccctttat
    21781 tttgagtagt atacttgaca acgatccggc gatccattgg aatagctgta tcagtttgat
    21841 aatattcctt atttagccag ctgcctgtta ctacgtgcca gaaactaagc agaaagacac
    21901 ctatcgcaaa accatttgga atcaagagga ccaataaaga agaactcagt ccaagcaatt
    21961 gcatagccaa tggagaccat gttaaacgtt tcaaaatcca tggtccgact atttttgctg
    22021 caatcaagcc tgctgcataa ggcaaataca taaaaatcga aagtttttct aatccgtaca
    22081 gtatgccaac ataaaaactg ctaaataaaa aaaggaaatt ccctagcatt ccatttagaa
    22141 aagtaaacag atttagccag cttggcaact tccattcctt cttctggtgt cctaaaacaa
    22201 acacgaacaa gatgaacagc agcaagaaac cataaatagc aagatcaaaa agcctttcat
    22261 cgaataacaa tcgggcattt ctcaaaagaa acaataagcc aaagaatatc aaaaataaaa
    22321 caagctctcg cgtagctacc agatttcttt tgatgtcttt gaaatccagt tcgtaacgcg
    22381 gataatgact aaccgtatgg taggccatga cgtaaaaaag tgtataaata gtaaagcttg
    22441 ctgcaatctg agtcggtgca ggcaactcaa tcgcttgata caggagaaat agccagatag
    22501 ctgcaaataa gtactttttc ccatccatat tgataaaacc ttgcgtcttt tcgtgaaaat
    22561 tgaccgtggt attagcaggt acaagccatg ctgcactcag ccctagcaaa acacttgaaa
    22621 aataaaataa aggaaaataa aatacgccta atattccaag caatgaacca gcaccaccaa
    22681 tcagcagagc gatcatcagc aacgtgtaac tatctagacc aaatcgtaga ctgttgacta
    22741 aaaatagtcc agtcatccga aaggcataaa acagcgtaaa tggcaaaatc gtcagccaat
    22801 tatgcgagtc tgttagtccc aaaaataaaa gataaggaat aaaaataacc gtgttactta
    22861 agataaaggg cgttgtttgt gtaaaattac gcccaaatcg tttgagtttt tcgaacatga
    22921 caatggctcc ttaaacagtc ttataactaa tctgctcttc gattttagca ttaaagggga
    22981 aataaaccaa ttttacagaa tatattcttg taaaaaaaaa tacagaaaga agcataagct
    23041 tctttctgca tttttacggt acttgattac tcaagcagac aagcccttct ccacaaaagt
    23101 cttgcccgac acccctgcat ccggcaggtg ataaagatag aaatcaatta tcctgtgcct
    23161 ccaaaatcaa ttaggagctc tgtaaaccag agtccttctc gacccgtcgc ataaatttac
    23221 tttaacacag ttcctatgtt tatgcgagag cttttttcat tttataatcg accatttgaa
    23281 atccttgacc gatatctgaa ataagttctt ctgtaatctc aaatccttca tgcttataaa
    23341 cctcgattgc ttgatgattc ccctgattca ctcgcaaaaa gatctctctt tcctttgttt
    23401 gcgcagcaag ttcttcatac cagcgaaaaa ctgaagaagt caatccttga ccacgtactt
    23461 tatttagcaa atataattta cttaaataaa tttttccatt tatctcttca taagcagtgt
    23521 aaccgacagt cttttcttcg ctttttaata aaaagtagga cacaccttct gtcagttctt
    23581 tttgaatctg tttttttgac tgataagttt tcagcatgta atccacttgt ttttgaccaa
    23641 tgatcggcac aaaagcttcc ggccatactt cttgtatgac tgccatcagc tcatccagtt
    23701 cattttctgt tacttttatt ttttccatac acccacgtcc ttttttacat tataaatgac
    23761 cttgcgcttg atggaataaa aagagcatga tcatgctata cacacccacc gaccatggtt
    23821 tcaagaaaat cacaatctgc ataaattccc taaaagataa tttcgtcaaa ccagctacta
    23881 gacagatcaa gtcgtctggc gcaaagggaa gcagcaaggt aacaactagt acttttttaa
    23941 tatctttcgt ttttcttgtc agcaattcat caaatttctg atactttttt ggcgataaaa
    24001 taagctggac aaaggctacg ccatagtaac ggacaagcaa aaatccgatt gcttccccaa
    24061 tcactaggcc aatacaacta tataaaagcc caaatccatt gccaaacatc agcatgcccg
    24121 ctactgagga gatccctccc ggcaaaatag gaatgatcac ttggatcact tgcaacagaa
    24181 caaatatcat cactgcatac ttgccaaatt gctggataaa cgcttgcaaa gacgtgctcg
    24241 atcgaaaaat cccatgatga taagcataaa gaatcaacac aaagaaaaaa aacagaccaa
    24301 taatgggcat gatctgaaga catttttgta aaactgatcc ttttttagac atctatattc
    24361 cccacatctt ttttcattta ttaaaactgc ctcctt
//
LOCUS       DAFEHS010000035        24301 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFEHS010000035
VERSION     DAFEHS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.035"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(132..2477)
                     /locus_tag="LP327_001519"
     CDS             complement(132..2477)
                     /locus_tag="LP327_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL1725639.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="LP327_001520"
     CDS             2756..3658
                     /locus_tag="LP327_001520"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBL1725640.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="LP327_001521"
     CDS             3869..5644
                     /locus_tag="LP327_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBL1725641.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="LP327_001522"
     CDS             complement(5710..7296)
                     /locus_tag="LP327_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBL1725642.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="LP327_001523"
     CDS             7547..7771
                     /locus_tag="LP327_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBL1725643.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="LP327_001524"
     CDS             7913..9664
                     /locus_tag="LP327_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1725644.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="LP327_001525"
     CDS             9664..11448
                     /locus_tag="LP327_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725645.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /locus_tag="LP327_001526"
                     /pseudo
     CDS             11783..12635
                     /locus_tag="LP327_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12732..14618
                     /locus_tag="LP327_001527"
     CDS             12732..14618
                     /locus_tag="LP327_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL1725646.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="LP327_001528"
     CDS             complement(14659..15561)
                     /locus_tag="LP327_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL1725647.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15791..17194
                     /locus_tag="LP327_001529"
     CDS             15791..17194
                     /locus_tag="LP327_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL1725648.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17191..18627
                     /locus_tag="LP327_001530"
     CDS             17191..18627
                     /locus_tag="LP327_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL1725649.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18772..19698
                     /gene="trxB"
                     /locus_tag="LP327_001531"
     CDS             18772..19698
                     /gene="trxB"
                     /locus_tag="LP327_001531"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBL1725650.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19911..21638
                     /locus_tag="LP327_001532"
     CDS             19911..21638
                     /locus_tag="LP327_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL1725651.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21654..22004
                     /locus_tag="LP327_001533"
     CDS             21654..22004
                     /locus_tag="LP327_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL1725652.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22098..22958)
                     /locus_tag="LP327_001534"
     CDS             complement(22098..22958)
                     /locus_tag="LP327_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBL1725653.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23050..23478
                     /locus_tag="LP327_001535"
     CDS             23050..23478
                     /locus_tag="LP327_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL1725654.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23640..23816
                     /locus_tag="LP327_001536"
     CDS             23640..23816
                     /locus_tag="LP327_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBL1725655.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23843..24289
                     /locus_tag="LP327_001537"
     CDS             23843..24289
                     /locus_tag="LP327_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBL1725656.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7700 a         4346 c         5174 g         7081 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaattataac
    15601 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    15661 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    15721 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    15781 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    15841 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    15901 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    15961 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    16021 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    16081 caaagacgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    16141 cgggatattc aaagcttttc gtaccgttcc agtagttttg gcaatcgttg aagacatgaa
    16201 gcgtttatgt ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga
    16261 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    16321 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    16381 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    16441 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    16501 catcccgttt ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg
    16561 ctattactat cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    16621 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    16681 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    16741 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    16801 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    16861 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    16921 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    16981 ttatggaaca gctttgcaag cgtttacaat caatccgctt atacgatcag gaaagacagc
    17041 aaaacggatc atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    17101 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    17161 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    17221 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    17281 gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    17341 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    17401 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    17461 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    17521 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    17581 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    17641 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    17701 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    17761 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    17821 catcatgagt tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag
    17881 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    17941 gatgtttggg cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca
    18001 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa
    18061 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    18121 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    18181 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    18241 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    18301 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    18361 gattatcgaa tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta
    18421 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    18481 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat
    18541 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    18601 aatggagaaa atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc
    18661 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    18721 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    18781 gtaatcgtta ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca
    18841 aacttatccg tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca
    18901 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    18961 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    19021 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    19081 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    19141 ggacgtggcg tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta
    19201 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    19261 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    19321 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    19381 ggaaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt
    19441 gatttggcag cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt
    19501 agagaagctg ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg
    19561 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    19621 actgctgttg gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta
    19681 gaagaaacaa ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact
    19741 cttttttttg cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga
    19801 taaaagaatc ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag
    19861 tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg
    19921 aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat
    19981 taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat
    20041 tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata
    20101 cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa
    20161 aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg
    20221 aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc
    20281 caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa
    20341 ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac
    20401 aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt
    20461 taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg
    20521 gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat
    20581 tggtggaaac aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa
    20641 aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag
    20701 aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact
    20761 ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa
    20821 cggatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag
    20881 tattgacagg aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacatcaat
    20941 cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc
    21001 ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat
    21061 tcattgctga aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat
    21121 tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag
    21181 cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg
    21241 acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga
    21301 caatgagcgg acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag
    21361 aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga
    21421 cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga
    21481 agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa
    21541 tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg
    21601 cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa
    21661 aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca
    21721 cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc
    21781 gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag
    21841 cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg
    21901 tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac
    21961 tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag
    22021 agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa
    22081 aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga
    22141 tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg
    22201 acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct
    22261 cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt
    22321 tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga
    22381 tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc
    22441 tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct
    22501 agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca
    22561 gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca
    22621 ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc
    22681 acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct
    22741 tgattgatca atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc
    22801 atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga
    22861 tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg
    22921 acaaaaaaag taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt
    22981 tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag
    23041 gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac
    23101 ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc
    23161 tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac
    23221 gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat
    23281 ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat
    23341 cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat
    23401 ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga
    23461 tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca
    23521 agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt
    23581 tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca
    23641 tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac
    23701 gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac
    23761 caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct
    23821 gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac
    23881 agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat
    23941 acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt
    24001 gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga
    24061 cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca
    24121 actttccgac gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc
    24181 atcaatgaaa gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc
    24241 agatggaaac caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt
    24301 a
//
LOCUS       DAFEHS010000036        23785 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFEHS010000036
VERSION     DAFEHS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.036"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..171
                     /locus_tag="LP327_001538"
     CDS             <1..171
                     /locus_tag="LP327_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL1725657.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="LP327_001539"
     CDS             406..642
                     /locus_tag="LP327_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725658.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="LP327_001540"
     CDS             649..2601
                     /locus_tag="LP327_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBL1725659.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3157)
                     /gene="dfrG"
                     /locus_tag="LP327_001541"
                     /pseudo
     CDS             complement(2673..3157)
                     /gene="dfrG"
                     /locus_tag="LP327_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
     gene            3519..4559
                     /locus_tag="LP327_001542"
     CDS             3519..4559
                     /locus_tag="LP327_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL1725660.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4657..4845
                     /locus_tag="LP327_001543"
     CDS             4657..4845
                     /locus_tag="LP327_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL1725661.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4903..5256)
                     /locus_tag="LP327_001544"
     CDS             complement(4903..5256)
                     /locus_tag="LP327_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1725662.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5787..6209
                     /locus_tag="LP327_001545"
     CDS             5787..6209
                     /locus_tag="LP327_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL1725663.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6206..6436
                     /locus_tag="LP327_001546"
     CDS             6206..6436
                     /locus_tag="LP327_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1725664.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6934..7134
                     /locus_tag="LP327_001547"
     CDS             6934..7134
                     /locus_tag="LP327_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL1725665.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7161..8354
                     /locus_tag="LP327_001548"
     CDS             7161..8354
                     /locus_tag="LP327_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL1725666.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8422..9987)
                     /gene="guaA"
                     /locus_tag="LP327_001549"
     CDS             complement(8422..9987)
                     /gene="guaA"
                     /locus_tag="LP327_001549"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL1725667.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10046..10969)
                     /locus_tag="LP327_001550"
     CDS             complement(10046..10969)
                     /locus_tag="LP327_001550"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL1725668.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11165..11770
                     /locus_tag="LP327_001551"
     CDS             11165..11770
                     /locus_tag="LP327_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL1725669.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12078..13094
                     /locus_tag="LP327_001552"
     CDS             12078..13094
                     /locus_tag="LP327_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL1725670.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13235..14536
                     /locus_tag="LP327_001553"
     CDS             13235..14536
                     /locus_tag="LP327_001553"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL1725671.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14582..15244
                     /gene="deoC"
                     /locus_tag="LP327_001554"
     CDS             14582..15244
                     /gene="deoC"
                     /locus_tag="LP327_001554"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL1725672.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15265..15663
                     /locus_tag="LP327_001555"
     CDS             15265..15663
                     /locus_tag="LP327_001555"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL1725673.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15729..16811
                     /locus_tag="LP327_001556"
     CDS             15729..16811
                     /locus_tag="LP327_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL1725674.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17023..18585
                     /locus_tag="LP327_001557"
     CDS             17023..18585
                     /locus_tag="LP327_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725675.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18578..19711
                     /locus_tag="LP327_001558"
     CDS             18578..19711
                     /locus_tag="LP327_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725676.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19711..20667
                     /locus_tag="LP327_001559"
     CDS             19711..20667
                     /locus_tag="LP327_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725677.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20766..21932
                     /gene="deoB"
                     /locus_tag="LP327_001560"
     CDS             20766..21932
                     /gene="deoB"
                     /locus_tag="LP327_001560"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL1725678.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21956..22774
                     /locus_tag="LP327_001561"
     CDS             21956..22774
                     /locus_tag="LP327_001561"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL1725679.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22791..23498
                     /gene="deoD"
                     /locus_tag="LP327_001562"
     CDS             22791..23498
                     /gene="deoD"
                     /locus_tag="LP327_001562"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL1725680.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         7732 a         4211 c         4962 g         6880 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttggaatgtc attctctttg ccaatcactc
     3121 tattcttatc catcgcagca atcaaagaaa ctttcattta taacacagcc tttttaacta
     3181 atttaccatc taatttctta tttctatcaa aaaacctttt ctcattatcc tgcccgttag
     3241 ttgaataagc aattttttct ataagttata ttatcataat accacaaaat cacaaatttt
     3301 cattcaatta tcattttcaa ttgcatttta taaatcattt tgtatgtaat ttggtatgta
     3361 agttaaaatg aaaaaagtag tccattttga agtacatttt gtcattttgt actccaaaac
     3421 tgaactactt acttgcatat atacgccttt atagtggagt actacattta cgagattcgg
     3481 ttagagtatc agaaaaagaa aaaataaaag ttacagaaat gtatacttca ataaatggtg
     3541 aattatgtaa gattgataga gcttattctg gagaaattgt tattttgcaa aatgagtttt
     3601 tgaagttaaa tagtgttctt ggagatacaa aactattgcc acagagaaaa aagattgaaa
     3661 atccgcaccc tctactacaa acaactgttg aaccgagtaa acctgaacag agagaaatgt
     3721 tgcttgatgc ccttttggaa atctcagata gtgatccgct tctacgatat tacgtggatt
     3781 ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg
     3841 cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata
     3901 tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt
     3961 tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg
     4021 agagctcggt ttctcttgga tacttaaatc aatcatttca aaatgcagtt atggaaggga
     4081 tacgctatgg ttgcgaacaa ggattatatg gttggaatgt gacggattgt aaaatctgtt
     4141 ttaagtacgg tttatactat agccctgtta gtactccagc agattttcgg atgcttactc
     4201 ctattgtact ggagcaagcc tttagaaaag ctggaacaga attgttagag ccatatctta
     4261 gttttaaagt ttatgcacca caggaatatc tttcacgggc atataacgat gctcccaaat
     4321 attgtgcaaa tatcgtaaat actcaactga aaaataatga ggtcattatt attggagaaa
     4381 ttcctgctcg atgtattcaa gattatcgca atgatttaac tttttttaca aatgggctta
     4441 gtgtttgttt agcagagcta aaaggatatc aggttaccac tggcgaacct gtttgccaga
     4501 cccgtcgtct aaatagtcgg atagataaag taagatatat gttcaataaa ataacttagt
     4561 gcgttttatg ttgttatata aatatggttt cttattaaat aagatgaaat attctttaat
     4621 atagatttga attaaagtgg aaaggaggag attgttatta taaactacaa gtggatattg
     4681 tgtcctattt gtggaaataa aacaagacta cgaatacgag tggatactat acttaaaaat
     4741 ttccctttat actgccccaa atgtaagaac gaaactttaa ttaatgttca aaaaatgaat
     4801 ataataacaa tcaaagagcc agacgccaag acgcagagcc gataatttga gaaatgaaac
     4861 tctcatctta tcggctcttt ttgtttatct gaattttact gactagcctt caatatttct
     4921 tgcttcattg ataccttttg ctagtgcttc cattaaggat agttctttgt ctgtaaagct
     4981 atccatgtat ttttctacct gtaaccttcg agtgctttta atcaaattat ctgtaggtaa
     5041 gaaaaattca tcaatagata catggagtaa tgatacaaga tcatacagca cttgtgtact
     5101 tgggtgttgt cctttgtttt ctatatttgt taaataacgt gggtcaattt caatcattgt
     5161 tcccacttgt tcacgagtga gaccccgttt cattcgagcc tctttaatcg ctaaaccaac
     5221 ggctctaaaa tcgtatttat cttctttttt acgcatacta aatcacctct atacatttta
     5281 ctgttcctat ataaccataa acaggtatag aaaaacatgt tatatagttt atgagttcct
     5341 atagaacact gtggaattta ttaatgcgat gaatccaatt tcaaatagaa aggtcgctga
     5401 ttttatttac ctatacaact ttataaaatt tagtcatact cctagaaagt agataaaatt
     5461 ggcgacttaa tggagggaaa agtcgccatg agaaagataa tctgtcatct agttctctta
     5521 attaagttct tatcaaaaaa acttatccac ctgtactgga tagatatgac catcaatgtg
     5581 gttttgcaga ttacatagat agaaaaatca aagcatatga acagagaaaa caatctgttt
     5641 atgtgctttt ttggtcactt aataaatatc ttttaggatt tctcttcatt tatgcaacaa
     5701 atcccccgtt cgtgtcgggg tttagtatga agggagataa agaagcacat gccactccct
     5761 ttcacgacag aaagggggtg aaaagcatga aaccatcttc ttttcaagaa accatagaaa
     5821 atcagtttga ctatatctgc aaacaagtta ttgaagatga acgaaaagat tacttcaaac
     5881 atctagacag actatcagca aaagaagtct cgttttctga tatagataac taccttgtca
     5941 actctttttc tactgttgat acgtacgtca ctgatttcca actatttaaa ttatttggat
     6001 taagaatcaa tgttgaaagt gatttattaa gtgaagccct gcgtcatctt tcagagaaaa
     6061 aacggaacat tattttactt cattacttta tggatatgag tgatgtggag attgcggagt
     6121 tactaaattt gaatcgttcg actgtttatc gtcatcgaat aagtggacta gccatgatta
     6181 aagaattcat gaaggagtgt aaagaatgaa aacagcttat ccaagggttc ccttccccct
     6241 cattgtaaag gcgacagatg gcgacgtgga agcaattaat cagattgtta aacattatcg
     6301 agggtatacc tcaaaacgtt cattacgtcg catgacagac gaatatggaa attctcatat
     6361 ggttattgat gaaaccctac gtggcagaat ggaaacgaaa ctcatctcta agattttatc
     6421 gttcgaaatt aaatagtccc attttttcgt ggaagcgtgt cgaatcgtat ccacgcttcc
     6481 cgatttggga ggtttgttat tccatcaaag catattgtgt gttcagtagg ttttgatagg
     6541 ctaacaagcc aaacgttctt tgacaaccaa ataaaggcaa tcgaatacgt ttcggtaaga
     6601 aaagagctga tgaactaaca cgccatgacc tacaaaatta gtgagcgatt catgttagta
     6661 attcgaaagt aatctttagc aggattgctg ccatgacttt cttatcgtga taatgatact
     6721 cccgtatagc catagtccga gcgttagaag cgtcacaggc aatgggtacg gctacatgag
     6781 aaccatgcag aagtgaaatt cctgtgagct ttgctagagc tgttcgattg ctaagatatt
     6841 cttctcttgt cagtgtatta ttataaaact atttgatata attataggtg aatattggag
     6901 ttttcctagc ctataattgg gaaaggagta ataatgataa cggtcacaaa aaaggattta
     6961 gttgagctag gatatggaac ttctttttca gcagacatca taaaaaaagc aaaagaattg
     7021 atgattacaa aagggcatat ctactatcaa tctcgaaagc tagatagagt ccctcgtgaa
     7081 gctgttgaag aaattctagg aattcggttt tcaaatgaaa acaggtctga gtagtttttg
     7141 cacagtaagg agtaaaaact atggttaaag acccaataaa aaaagcaaag aatggaacct
     7201 attattttcg tgcaaatcta ggttatgatt ctaaagggaa aaaaattcaa aaatatcgta
     7261 gtggattcac tactaaaaaa gaagcaagag aagaatattc gaaactgcta ttgatgaaac
     7321 cagaagaatt aagtgagaat aaggataaaa tgacgtttaa acattatatc tttgaaattt
     7381 tccttccatg gtataagaca caggtaaagc tcagaacata tgaaaatcgc ttaccaacta
     7441 ttaagaagca tttctcttat tttgataaaa tggctgtttc cgatattgag cctattgatg
     7501 tgcaaaattg gcaactaaaa ttatcaaaga agtgtaagtc ttcttatgta agagctgttc
     7561 aaggactatt ttcagttgct atggatcgag caattgtcct tgggataacc accacaaatc
     7621 catctaagat tattggcaat gtgaaaaagc aaaagtctaa aattgaattt tggacaaaag
     7681 aagaatttga aaaagtaatc tctctaattt acaaagaaga ttactatcaa cactttttat
     7741 ttatctctct gtggttttta tttatgactg gaatgcgaat tggtgaagct actgccattc
     7801 aatgggaaga tattgatttt gactcaggag tactaaccat caacaaaaca ctctattaca
     7861 agaatcaatc gaattataga tttgttgaac caaaaacaaa ggcgagcgtt cgtcatattg
     7921 tattagataa atgcacatta acttatttat ccgaatggaa ggaagtacaa caaaatctaa
     7981 ttcagacaga ctttgtaatg agctataacg gtattcctac ccaaaaacat acactatcat
     8041 atgctattga acgctatgct aagctggctg gtatccatcg aatcagaatc catgggttac
     8101 ggcattctca tgcttccttg ttaatcagta tgggagaaaa tccacttatc attaaagatc
     8161 gtctaggaca cgaagatatt gagacgactt taggaacgta tggacatctt tatcctaaca
     8221 gtaatttcga ggtagctaac aaattaaatg gagccatttc ctttaaagaa gcaaaacaaa
     8281 attatgatac ttcaccaaag aatcaattta ctgtagacta cttgcgaaac aaagaaatga
     8341 aaaaagtgca ataaaagtgc agtaaaaaga taagaagagc cagaaaccct tggtataaga
     8401 ggatttctag ctctcctaca actattccca ctccactgtt gctggtggtt tagaagtgat
     8461 gtcatacacg atacggttga cgtgggctac ttcgtttacg atacgtacag agattttttg
     8521 caagacttcc catgggatac gggcaaagtc agcggtcatt ccgtcgattg aagttacagc
     8581 acggataccg actgtgtagt cgtaagtacg tccgtcaccc ataacaccga cagagcggat
     8641 ccctggaaga acagtgaaat attgccagat atcacggtca agtccagcag ctgcgatttc
     8701 ttcacgcaag atagcatcag attcgcgtac gatttctagc ttgtcttcag tgatttcgcc
     8761 aagaacacga ataccaagac caggaccagg gaatggctga cgccaaacga ttgaatcagg
     8821 catgcctaac tgagtcccta attcacggac ttcgtctttg aataatgtgt ttaatggttc
     8881 gatcaattca aattgcatat cttctggcag tccacctaca ttatggtgag atttgatcgt
     8941 ctgtgctgtt tctgtaccag attcgataac gtcagtatac aacgttcctt gtgctaggaa
     9001 agagacgcct tcttcaccag caagttttgt tgcttcgtca tcaaaaacat atacgaattc
     9061 attcccaatg attttacgtt tttgttctgg gtcagataca cctttcaact tgtctaagaa
     9121 gcgatctctt gcatcgactt tgatgatgtt caagccgaat tttccgccta gactttccat
     9181 tacttgttcc gcttcgcctt tgcgtagcag accgtggtca acaaagatag atgtcaattg
     9241 gtcaccgatc gctttttgta aaagaacccc aacgacacta gaatcaacgc cacctgaaag
     9301 accaagcaag acttttttgt caccaacttg ttcacggatt ttttctactt gcatatcgat
     9361 aaagttatcc attgtccagt taccttcaca gtgacaaaca tcaaaggcaa agtggcgcag
     9421 caattcgatt ccgtattcgg aatgacgtac ttctgcatgg aattggatac cataaaaatt
     9481 acgtgttgtg tcttggatag aagcaatcgg acaatctttg cttgttccga ctgtttcaaa
     9541 gccttcagga actttagtta ccaagtcacc atggctcatc catactgttt gttgttttgg
     9601 tgtttcttta aacaagacag cttgatcgct tgttacttct aacaaggctt tcccatattc
     9661 gcggttttta gcaggctcta cggaaccgcc aagtttgtac gtgatcaatt gcataccata
     9721 gcaaatccct aatacaggaa tacctaaatt gaagatctca ggatcgatcc caaatgcatt
     9781 ctcatcatat acactgtttg gtccgccaga aagaatgatt ccctttggat tcatcgcttt
     9841 tacttcttct gcagtaatgc ggtggctcaa taattcggaa aagactccaa gatcacgaat
     9901 acgtcttgtg atcaattggt tatactgact accgtagtct aatacgataa tcttttcaac
     9961 gttcgtcata tcggcaacgt ttgtcaaatt tattacccct atccctttaa attttatctc
    10021 aaaatagtca aactatttta aaatgctaat attttcgcat gaaaatcgaa tcgatgatat
    10081 gattttcagt tttatggaga attatatcgg ctcttcctcg tgtaggcaag atatattcct
    10141 gtaaattttt caaattcaca tttttccata catcttttgc cattttaaag gcttcttcac
    10201 gatctccgat agcaaaacga taataataat tctctggatt ttggaaagcc gtatccagta
    10261 acgcaccaaa acgatccaaa taccattttt cgatcagcac gggatcggca tcgacataga
    10321 cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt cgcaggtaat tgaagcgtat
    10381 tgatcccctc aacgatcaag atatcaggct gctggatcaa ttcgtacttg tctggaatga
    10441 tatcatacac atcatgtgag taaacaggta cttttatctc ttcctgacca gattttacac
    10501 ggttcaagaa atcgatcagc atctccatat cataactttc aggaaatcct ttacggtcca
    10561 tgattccacg ctcttttaat atacgatttg gataaagaaa tccgtcagtc gtgatcagct
    10621 gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg cagcagtctg gctgttgtac
    10681 tttttccaac ggctacactt ccggcgatcc cgataataaa aggcggtact tctacgtatt
    10741 tatgaagaaa taaaccttta ctcaagaaca aagattcaaa ttctttcata taaagataaa
    10801 tcagatgagc aagaggtaca tagatatctc gaacatcctg catggaaatg cggtcattca
    10861 aacttttgat attatctagt tcatcttgtg tcaaaggtgc ttgcccatta cgataaaatt
    10921 ctcgccattc ttctcttgcc acacggtagt agttcattgg atcattcata ttagcctcct
    10981 gcgttctccc atcaatggaa ctattttaac acaaaataaa cgcaaacccc accacttaag
    11041 agagattgcc gatctttttg tataatcttt ataatagaaa gaaacggagt cttattcttg
    11101 acttgcgcac ggatacataa aaggatagac tgttgtcatg aaagaaatga gaaggatgga
    11161 agacatgaca aatcattact attctgaacg cccagatacg gcgcacgatt ttgaacaatg
    11221 ggcttttgag ttaaaaggca gaacctttca atttgtaaca gattctggtg tattttcacg
    11281 tgaaacagtg gattatggtt cacgtgtcct gattgatgct ttcgattggg aagagttacc
    11341 tgatgaagga aagatcttag atgtgggatg cgggtatgga ccgattgggt tagctttggc
    11401 atttgcgaca cagcggtttg ttgaaatggt cgacattaat gaacgagcgg taagtctagc
    11461 gcaaggaaac gcaaagcgta accaaatcga acatgttgat atccatcaat cgaatatcta
    11521 tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc aatccgccga tccgtgctgg
    11581 aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct cggttgaaga agggcggtac
    11641 actgacaatt gtgatccaga agaaacaagg agcgccgagt gcgcaaaaga aaatggaaga
    11701 aacatttggt aatgcagaaa tcgtcacaaa agataaagga tattatatca tcaaaagtgt
    11761 gaaagaataa ctagctgaaa aatagtggag cgacgaaaga gtggaactgg gcgtgcatgt
    11821 acgggtacgt atcagttctt tttttgttga gaagttttta aaaacaaaga actatttttt
    11881 cttcaaaaag aaatagtagg aacagagtcg ttgtttttta cgataaaata aataagatac
    11941 agaacttgta tagaaggctg ccaagtcggt agataaagga ggtttttatc aaaaaacata
    12001 ctttcctttt ttctctattc ttttcttggg ggttttggta cactgatagg gaatttagat
    12061 agaagggagc caataggatg aagctgacca tcagagatat cgcagaaatg gcaggcgtat
    12121 cagtaacaac tgtctcacag attttgaata acaaaggaag cagatttagt gataagacaa
    12181 gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa gcccgattat ttcgcttcaa
    12241 atgtgatcaa tcgacattca aagaccatcg gaatgatcgt ccctgatgtc actgatttct
    12301 tcttctccaa actgatcgaa ggtgtagaga gctatttgaa tcctttgggc tatgtgatca
    12361 tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa gtatttacaa gagctttccc
    12421 accgctcagt ggacggaatc ttgctggcca caccaaatat tttacctgag aaatatgcat
    12481 tagacagcgg tttttatcaa aaaatgccaa tcattttaat cgatcgcgga ttgaatcgtc
    12541 gggataacgg acggcttatc gtaaaggaat atgagggcgc ctatcaagct gtttcgctgt
    12601 ttattgaaaa tggccatacg aagatcggca tgctgaagga aaccactgga tactatcaat
    12661 tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg ccttttaatg
    12721 ggaaatttgt tgaacatgga gagttgactg tccaaggtgg atatactgca tcaaaaaaat
    12781 tactcaaaca taaagatatc acggctattt tttgcgggaa tgatgccatg gcgatcggat
    12841 gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga agatatctca gtcataggat
    12901 tcgatggatt aaagctgtca gaatacatga ttccgcaatt gacgacagtg cagcagccaa
    12961 gttttgatat tggattttat gctgcacgtt tcctgatcga tacgatcgaa tttcctcaaa
    13021 gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc gtaaaagtat
    13081 tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc atttacaaag gctagttagt
    13141 gcttttttta tttttagtaa accagtttac taaagtggtt tacctttcct aaaaataatg
    13201 tataataccg aatgaagaca aggaggatca ggcaatgcga atggtggatc tgattgaaaa
    13261 aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca cgaactatac
    13321 gaatggaaag attccagatt atcaagtcag tgcattattg atggcaattt tttatcaaga
    13381 catgacaaat gaagaaatca cgaacttaac attggcgatt gctaattctg gtgatgtcat
    13441 cgatttgagt tctcttgaag gaataaaggt agataagcat tcgacaggtg gcgttggtga
    13501 cacgaccaca ttgattttag cccccctagt agctagtgtc ggagtgacag tggctaaaat
    13561 gtctggacgc ggtctaggtt acacaggtgg tacactagac aagctagaag caattcccgg
    13621 atttcagatc gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa gtaaagtagc
    13681 ggtaattggt caatcaggaa acttggcacc ggcggacaaa aaattgtatg ccttgcgtga
    13741 cgtcacagcg accgttgatt ctcttccgct gattgccagt tcgattatga gtaaaaaaat
    13801 cgcggcagga gcagatgcaa tcgttttgga tgtgaccact ggagacggtg ctttcatgaa
    13861 gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg agcattggga aactggctaa
    13921 ccgagaaaca gtggcggtta tctcagatat gtcagaacct cttggcgaag ctatcggaaa
    13981 tagtctagaa gttgtcgagg caatcgaaac attacaagga aatggtccag aagatctagt
    14041 agaaatgtgc tacgccttag gaagccaaat ggttgttctt gctggcaaag ctaagacaac
    14101 cgatgaagca agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt tagcaaaatt
    14161 caaagaaatg atccaaaatc aagggggaga cccagcgatc gtggaacatc cagaacgaat
    14221 tttgactgca cgctacacaa tggagcttcc tgctaaacaa agcggagttg tgtcaaaaat
    14281 cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt gctgggcgta aaaccaaaga
    14341 agaagatatt gatcacgctg ttggattgaa actgcataaa aaaattggtg acactgtgac
    14401 aaaaggtgag tcactattga cgatctatag taacgacgaa gaaatcactt ctgtcattga
    14461 attattatat aaaaatattg aaattggtga atcggcaatg aagccaacgt tgattcatga
    14521 catcatcaca gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa ggggaaaaga
    14581 aatggaacta aaccgaatga tcgaccatac tattttgaaa gcagacgcat ctaaagaagc
    14641 tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt tattctgtct gcatcaatcc
    14701 agcatgggtt gcgctagcga aagaacaatt acaaggtgag ccagtagccg tatgtacagt
    14761 aatcgggttt cctctaggag caaacacacc cgagacaaaa gcatttgaaa caacagatgc
    14821 catcaataac ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt tgaaatcaaa
    14881 ggatgatcaa caagtccaaa aagatatcga agcagttgtt gaagcagcaa aagatcgtgc
    14941 gcttgtcaaa gttattattg aaacatcgtt attagataga gaagaaatca tccgagcttg
    15001 cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt tctcaacagg
    15061 cggagcaaag gtagaggatg tccgtttgat gcgtgaaact gtaggaccag aaatgggtgt
    15121 caaagcttcc ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg aagctggtgc
    15181 tacaagaatc ggaacaagcg caggagtagc aatcgtatct ggttcaactg gtgaaggcta
    15241 ttaatttttt caaaggagaa gctcatggca gaactcaaac aagaatggat caacacagct
    15301 attgaagcgt tagataaagc ctatgttcct tattcccatt ttccggtagg cgcttgtctc
    15361 gtaacggaat caggcaagat ttatcaaggc atcaacattg agaatgcatc atttggatta
    15421 acgaattgtg ccgagcgcac agcttttttc aaagcagtat ctgaaggaga aaaaacattt
    15481 acgcatctag tcgtagcggg gcacaccccc gatcctatct ctccttgtgg tgcatgtcga
    15541 caagtaatgg cagaattctg ttcccctgat atgccggtta cgctagtagg agacaatggt
    15601 gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg catttactga aaaagatcta
    15661 taactcatgt tcattcagtc agctgactgt tgaaaaataa aacaattatt ttaggaggct
    15721 ttacagaaat gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta gcagcagggt
    15781 tatttttagg tgcatgcggc aacaacggct ctactgattc atccggtggc aaaagctcat
    15841 cagacacaac aactgcagca ttgatcacag acacaggtgg tgtagacgac cgttcattca
    15901 accagtcagc ttgggaagga ctagaaaaat gggggaaaga tcaaggactt tcacgaggaa
    15961 atgatggctt ccaatatttc caatcttcaa atgaatcaga ttacatccca aatattgacc
    16021 aagcattgaa tgctggattc aaaacgattt ttggtattgg ttataaacta aaaccagcga
    16081 tccaagaaca agcaacaaac aatacaggta cgaactttgt tattatcgat gatgtcatcg
    16141 atggattaga taacgttgta tctgcaactt tcaaagacaa cgaagcttct tatcttgcag
    16201 gtgttgcagc agcttatacg actgaaacaa acgtagtcgg ctttatcggc ggtgtaaaag
    16261 gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg tgttgatgca ggtgcgaaag
    16321 aattaggaaa agaaatcaaa gtattgaatc aatatgctgg tgacttctct gctccagata
    16381 aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc agatatcatc ttccatgctt
    16441 caggcggtac aggtaacggt gtattccaag aagcaaaatc attgaacgaa tcaggcgaca
    16501 aaaaagtttg ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa tacacattaa
    16561 acggcgaaaa gaaaaacttc acattgacat ctacactgaa agcagttgga acagtagtag
    16621 aagatttggc acaaaaatca gcggacggta aattccctgg cggcgaacac actgtttatg
    16681 gattgaaaga agacggtgtt ggtctaacag aaggacaact ttctgacgaa gcgaaaaaag
    16741 cagttgatga agcaaaagaa aaaatcattt caggtgacgt aaaagttcca gaaactcctg
    16801 aagaaaacta aaaccatttc tatagccttg ttagacacat gtttgacaag gctatggttt
    16861 gattagaaca agaattgttc atttatccaa gctgccttgt gacagtttgg ataagggaac
    16921 aactccctga agtaatgttt gtttcaaaag aagaacgctc actaagcgtg aatcttagtc
    16981 aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa aggtggttca agaaaatatt
    17041 gtcatagaaa tgcagaatat taccaaagaa tttggaacat tcaaggcaaa tgacaacatc
    17101 aatttacaag taaaggcagg agaaattcat gcgttgcttg gagaaaacgg tgctggcaaa
    17161 tctacattga tgaacgtgct ttccggatta ttagagccga catcagggaa aattttgatg
    17221 cgtgggaaag aagtaaagat cacaagtccg accaaagcca accaattagg gattgggatg
    17281 gtccatcagc actttatgct tgttgatgcc tttactgtaa cagaaaacat cgtgttggga
    17341 agcgagccta gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga gatccaaaaa
    17401 gtttctgaac agtatggatt gtcagtcaac ccagatgctt acgttcgtga tatttcagtt
    17461 ggaatggaac aacgggtaga aattttaaaa acactttacc gaggagcaga tgtactgatt
    17521 tttgatgagc cgacagctgt attgacccct caagaaattg atgaattaat cgtgatcatg
    17581 aaggaattag tcaaagaagg caagtcgatc attttgatta cgcataagtt agatgaaatc
    17641 aaagcagtag ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg tacagtcaac
    17701 gttaaagacg taacctcaca acaattagcc gatatgatgg ttggaagaac ggtttcattc
    17761 aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga aaatctagtg
    17821 gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga acttagaggt tcgtgctggc
    17881 gaagtacttg gtatcgctgg aatcgatgga aacgggcagt cggagttgat ccaagctttg
    17941 actggtttgc gaaaggcaga aagcggacat atcaagctaa aaggggaaga catcactaat
    18001 aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg tgccagaaga ccgtcataaa
    18061 tacgggttag tcctagatat gacattatct gaaaatatcg cactacaaac ctatcatcaa
    18121 aaaccttaca gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca tgccagagaa
    18181 ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa agctttatca
    18241 ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg accgagatcc tgatctgttg
    18301 attgttgcaa atccaactcg tggactggat gtaggtgcga tcgaatttat tcataaacgt
    18361 ctgatcgaac aaagggacaa atacaaagca gtgttactga tcagtttcga attagaagaa
    18421 attttaaatg tttcggatcg tattgctgtt atccatgaag gagaaattgt cggtatcgtt
    18481 gatccgaaag aaacatctga aaatgaatta ggtctattga tggcgggcta taccttagaa
    18541 gaagcacgaa aagagttaga aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt
    18601 ccgtaattta ttagtgcctg tactttcagt tgttttagga ttgatcttag gcgcgatatt
    18661 gatggctgct tttggttttc agcccatcca aggttattct tctatgctag atgcagccct
    18721 tggttcgcaa agagggatag gtgaaacgtt aaggcaagca acgccgttga tttttacagc
    18781 attaggtttt tctgtagcta actcagctgg gtttttcaat attgggcttt ctggacaagc
    18841 actttgcggg tggatcgtca gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt
    18901 tgttttgctt ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg cagcggctgt
    18961 tccaggttta cttcgagcat tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa
    19021 ctatatctta ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg aaagctatcg
    19081 ctcaagtttg gactcttcca atatgatcag tcaaaatgcc tcactgcggt taccatggtt
    19141 aacacaagct tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct
    19201 tattttcgtt tggctgctga tgaagaaaac gactttagga tttgaaatcc gttctgttgg
    19261 attgaatcct tttgcttctg agtatgcagg aatgagtagt aaacgtacga tcgttttgtc
    19321 gatggtcatt tctggcgctt tggcagggct tggcggtgtg gttgaaggat taggaacgta
    19381 tcaaaacttc tttgttcaaa cgacttcgct atctattgga tttgacggaa tggctgtttc
    19441 cttgcttggt tctggatcag caatcggtat tcttttatca gcgatcttgt tcagtatttt
    19501 gaagatcggt ggacttggca tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt
    19561 atcgattgct ttgattatct tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc
    19621 taaagtcttc caagggaaaa aacaagaaga gatcgttgcg acaatagaat cgaaaccaac
    19681 gaatcaaaac catgaagggg gcgaactata atgtcgacta tcgaattaat tgccttgatc
    19741 cttacttcta cactggttta ttcaacaccg ctaattctaa cgtcactagg cggaactttt
    19801 tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat gggtgctttt
    19861 agttccatcg ttttcaactt gacgatgagt ccatcacttg gttctatgac accatggatc
    19921 gctattttag tgggtggcgt cgtaggtatc ctattttctc tgattcacgc agtagcaaca
    19981 gtcagtttgc gtgctgacca tattatcagc gggacagtaa ttaatctgat ggcacctgct
    20041 ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc aaactgataa catcacgcag
    20101 aatctggggt atttttcttt cccaggatta gcagatattc cggtcattgg accaattttc
    20161 tttaaaaata cattcttacc tgcttattgt gctattttga ttgctgtcct agcttgggtg
    20221 atcatcttca agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct
    20281 gccgacacat taggtatcaa tgtatatgct atgcgttatt caggcgtact gatttctggc
    20341 ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga tttccggtaa cttttcagct
    20401 ggtacgatcg ttggacaagg gttcatctcc atggctgcta tgatctttgg taagtggaat
    20461 cctttagggg caatgggtgc ctcattgttc tttggttttg ctcaaagctt gagtatcatt
    20521 ggcgcacaat taccagttat ttcttcgatt ccgccagtat tgctgcaaat tgctccatat
    20581 ttgctgacaa tcattgtgct ggttgttttc ttagggaaag cttctggtcc aaaagcaaac
    20641 gggaagaact atatcaaatc aaaataatct tttagcgaat aaaaagggtg ggttacggga
    20701 agtgtttttc gtctcgccct ttttgtttta aaggcaataa aaataaaaag aaagaaggaa
    20761 taatcatgtt taaacgcgta catctgatcg tcatggattc agtaggaatt ggagaagcac
    20821 cagatgccga taagttcggt gatgttggtt ctgatacatt aggacacatt gcgaaagaag
    20881 caggattgaa cgttccacat ttagagcagc ttggtttagg aacgatccgt cctcttgaag
    20941 gtgtggaatc agtgaaagat catcaaggct atgcgacgaa attagaagaa gtttctgtag
    21001 ggaaagatac gatgacaggc cactgggaaa tcatgggatt gaatatcaaa actccatttc
    21061 gtgtgtttcc taatggattc cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc
    21121 gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt tattgacgat tacggtgaac
    21181 accaaatgaa aacaggcgat ttaattgtat atacttctgc cgatcctgtc cttcagatcg
    21241 cggcacatga agaaatcatt cctttagaag aattatatcg gatttgccaa ttcgttcgtg
    21301 atataacaaa agatgatccg tatatgattg gccgaatcat tgcacgtccg tacgtgggtg
    21361 aaccaggaga cttcacacga acaagcaatc gccatgatta tgcgttgaat ccattcggac
    21421 acacagtcct tgattctttg aaagaagcag gaaaggacgt gattgctgtt ggaaagatca
    21481 acgatatttt caacggacaa ggaatcactg aatctgtccg cacgaaaagc aatatggatg
    21541 gcgttgacca gctgctgaaa gttatgaaaa acgatttttc aggaatcagc tttaccaatt
    21601 tagtagattt tgatgcatta tacggtcatc gtcgggatgt agtcggctat gcgcatgcga
    21661 ttgaagaatt tgacttacga attccagaat tgcttgaagc gatggaagaa gacgacttgc
    21721 taatgattac agccgatcac ggaaacgatc cgacgtttac tggtaccgat catacgcgag
    21781 aatatgtacc gttattggtt tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag
    21841 gatattattc agatatttca gcaacgattg ctgagaactt tagtgtttct tcgactgaaa
    21901 atggcgaaag cttcttgaaa ttattaaaat aatcttttta aataggagaa agcaaatgac
    21961 caagttaagc gagatgttac aagaaacgac ccagtttatc cttgataaag gagtaaaaga
    22021 aattgatttt ggtttgattc taggttcagg attaggtgaa ttagcagatg aaatcgaaga
    22081 tgctgtaatg atttcttatg atgagatccc gttctttcct gtttcgacag tagttgggca
    22141 tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa gtgttagcca tgcaagggag
    22201 attccactac tatgaaggtc attccatgca aacagtgacc tatccagtac gtgtgatggc
    22261 cggactaggg gcccattcag tcattgtgac taatgcatgt ggtggtgtca acgaatcatt
    22321 tgtaccagga gatttgatgc tgattaccga tcatatcaat tttactggtg aaaatccttt
    22381 gatcggtccg aatgaagatg caatgggccc tagatttcca gatatgagtc aagcttatac
    22441 gcctgactat caagcaacag caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga
    22501 aggcgtatat atgggctatt ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc
    22561 tcggacaata ggagccgatg cagtagggat gtcgactgtt ccagaagtca tcgtagcagc
    22621 tcacagcgga ttaaaagtgt taggaatctc ttgtattacc aacttagcag caggtatgca
    22681 agcgaatctg agccatgaag aagttgtaga aacaactcag agagtcaaac aatcttttaa
    22741 agcattggtt aaagaaacat tgacattatt gtaataggag gaacaataaa atgagtgtgc
    22801 atattgaagc gaaagaaggc gagatcgccg ataagatttt acttccggga gatcctttgc
    22861 gagctaaata tattgcggag acttttttgg aagatccagt ttgttacaat caggtacgcg
    22921 gtatgttagg ctttaccggc aaatataaag gagaacgtat ttctgttcaa ggaacaggaa
    22981 tgggaatgcc atcagccacc atctatgccc atgaattgat ccagtcgtat ggtgtaaaga
    23041 aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt cgtgatttag
    23101 tactagcaca aggtgcagcg accagttctt cgatgatcga aaaaaatttc caagcctttc
    23161 attttcctcc aatcagtgat ttcaatctat tactgaaagc atatgagata gctaaagaaa
    23221 agggctatac ggttcatgta ggaaatgttc tttcggaaga ttctttctac aaagatgatt
    23281 tgacagagac gttccaatta gctgaacttg gcgttttagg agtggaaatg gaagcagcgg
    23341 ctctttatta cttaggcgcg aaataccatg tgcagacatt aagtttgatg accgttagcg
    23401 accatttgat caccggtgaa gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa
    23461 tgatcgaagt aggattggaa acagcgatag cagaataaga aggaaatgaa aaagctgccc
    23521 tgcattaagt aataagaaca tccaagcaaa aatagctcct catgtttttg acttggctgg
    23581 acttattatt gcaaatgtaa gcttttgaac accgtttacc aagaggttgt gaatctgccg
    23641 tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt gatgaatatt
    23701 gtgacttgtg accgagaagt tggcaggaga aaaccgttta tacgaaaaaa gggactgtga
    23761 caaatcgtgt cacagtccct ttttt
//
LOCUS       DAFEHS010000037        23659 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFEHS010000037
VERSION     DAFEHS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.038"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            257..532
                     /locus_tag="LP327_001563"
     CDS             257..532
                     /locus_tag="LP327_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725681.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="LP327_001564"
     CDS             545..2623
                     /locus_tag="LP327_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBL1725682.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMMQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="LP327_001565"
     CDS             2783..4669
                     /locus_tag="LP327_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL1725683.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="LP327_001566"
     CDS             4681..5628
                     /locus_tag="LP327_001566"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL1725684.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="LP327_001567"
     CDS             5648..6175
                     /locus_tag="LP327_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL1725685.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="LP327_001568"
     CDS             complement(6238..6891)
                     /locus_tag="LP327_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBL1725686.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="LP327_001569"
     CDS             7023..7865
                     /locus_tag="LP327_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL1725687.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="LP327_001570"
     CDS             8023..8919
                     /locus_tag="LP327_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL1725688.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="LP327_001571"
     CDS             8922..9635
                     /locus_tag="LP327_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBL1725689.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="LP327_001572"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="LP327_001572"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL1725690.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="LP327_001573"
     CDS             10170..10397
                     /locus_tag="LP327_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBL1725691.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="LP327_001574"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="LP327_001574"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL1725692.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11683)
                     /locus_tag="LP327_001575"
     CDS             complement(11183..11683)
                     /locus_tag="LP327_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL1725693.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="LP327_001576"
     CDS             complement(11962..12360)
                     /locus_tag="LP327_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL1725694.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="LP327_001577"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="LP327_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBL1725695.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="LP327_001578"
     CDS             13473..14240
                     /locus_tag="LP327_001578"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBL1725696.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="LP327_001579"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="LP327_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBL1725697.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="LP327_001580"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="LP327_001580"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBL1725698.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="LP327_001581"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="LP327_001581"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBL1725699.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="LP327_001582"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="LP327_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBL1725700.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19838..20386
                     /gene="hslV"
                     /locus_tag="LP327_001583"
     CDS             19838..20386
                     /gene="hslV"
                     /locus_tag="LP327_001583"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBL1725701.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20400..21800
                     /gene="hslU"
                     /locus_tag="LP327_001584"
     CDS             20400..21800
                     /gene="hslU"
                     /locus_tag="LP327_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBL1725702.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21818..22609
                     /gene="codY"
                     /locus_tag="LP327_001585"
     CDS             21818..22609
                     /gene="codY"
                     /locus_tag="LP327_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBL1725703.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22691..23566
                     /locus_tag="LP327_001586"
     CDS             22691..23566
                     /locus_tag="LP327_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL1725704.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8191 a         4078 c         4984 g         6406 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caattaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatgca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    19861 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    19921 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    19981 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    20041 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    20101 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    20161 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    20221 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    20281 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    20341 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    20401 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    20461 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    20521 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    20581 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    20641 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    20701 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    20761 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    20821 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    20881 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    20941 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    21001 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    21061 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    21121 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    21181 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    21241 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    21301 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    21361 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    21421 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    21481 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    21541 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    21601 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    21661 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    21721 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    21781 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    21841 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    21901 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    21961 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    22021 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    22081 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    22141 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    22201 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    22261 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    22321 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    22381 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    22441 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    22501 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    22561 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    22621 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    22681 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    22741 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    22801 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    22861 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    22921 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    22981 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    23041 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcgattt
//
LOCUS       DAFEHS010000038        23571 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFEHS010000038
VERSION     DAFEHS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.039"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="LP327_001587"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="LP327_001587"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBL1725705.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="LP327_001588"
     CDS             complement(1172..1867)
                     /locus_tag="LP327_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBL1725706.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="LP327_001589"
     CDS             complement(1880..2596)
                     /locus_tag="LP327_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBL1725707.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="LP327_001590"
     CDS             2926..3531
                     /locus_tag="LP327_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBL1725708.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="LP327_001591"
     CDS             3856..4179
                     /locus_tag="LP327_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL1725709.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="LP327_001592"
     CDS             4361..5731
                     /locus_tag="LP327_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBL1725710.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="LP327_001593"
     CDS             complement(5861..7162)
                     /locus_tag="LP327_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL1725711.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9350)
                     /locus_tag="LP327_001594"
     CDS             complement(7392..9350)
                     /locus_tag="LP327_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBL1725712.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="LP327_001595"
     CDS             complement(9527..9793)
                     /locus_tag="LP327_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL1725713.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="LP327_001596"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="LP327_001596"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBL1725714.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="LP327_001597"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="LP327_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBL1725715.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="LP327_001598"
     CDS             13137..13700
                     /locus_tag="LP327_001598"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL1725716.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="LP327_001599"
     CDS             complement(13953..14474)
                     /locus_tag="LP327_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBL1725717.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="LP327_001600"
     CDS             complement(14477..15319)
                     /locus_tag="LP327_001600"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBL1725718.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="LP327_001601"
     CDS             complement(15312..16037)
                     /locus_tag="LP327_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBL1725719.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="LP327_001602"
     CDS             16213..16509
                     /locus_tag="LP327_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725720.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="LP327_001603"
     CDS             17077..18669
                     /locus_tag="LP327_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL1725721.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="LP327_001604"
     CDS             18682..19422
                     /locus_tag="LP327_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL1725722.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="LP327_001605"
     CDS             19590..20939
                     /locus_tag="LP327_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1725723.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="LP327_001606"
     CDS             complement(21067..21657)
                     /locus_tag="LP327_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL1725724.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="LP327_001607"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="LP327_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL1725725.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6749 a         4746 c         4491 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac agtactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DAFEHS010000039        23525 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFEHS010000039
VERSION     DAFEHS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.040"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(53..787)
                     /locus_tag="LP327_001608"
     CDS             complement(53..787)
                     /locus_tag="LP327_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL1725726.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="LP327_001609"
                     /pseudo
     CDS             1114..1524
                     /locus_tag="LP327_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
     gene            1517..2218
                     /locus_tag="LP327_001610"
     CDS             1517..2218
                     /locus_tag="LP327_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL1725727.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="LP327_001611"
     CDS             2218..3804
                     /locus_tag="LP327_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1725728.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="LP327_001612"
     CDS             3823..4044
                     /locus_tag="LP327_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBL1725729.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="LP327_001613"
     CDS             4041..4802
                     /locus_tag="LP327_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBL1725730.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="LP327_001614"
     CDS             5182..5691
                     /locus_tag="LP327_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL1725731.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="LP327_001615"
     CDS             complement(5766..6305)
                     /locus_tag="LP327_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBL1725732.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="LP327_001616"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="LP327_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBL1725733.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="LP327_001617"
     CDS             7318..8169
                     /locus_tag="LP327_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBL1725734.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="LP327_001618"
     CDS             8197..8832
                     /locus_tag="LP327_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBL1725735.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="LP327_001619"
     CDS             8852..9625
                     /locus_tag="LP327_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL1725736.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="LP327_001620"
     CDS             10033..10830
                     /locus_tag="LP327_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBL1725737.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="LP327_001621"
     CDS             10808..11221
                     /locus_tag="LP327_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL1725738.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="LP327_001622"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="LP327_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL1725739.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="LP327_001623"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="LP327_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBL1725740.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="LP327_001624"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="LP327_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBL1725741.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="LP327_001625"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="LP327_001625"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBL1725742.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="LP327_001626"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="LP327_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBL1725743.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="LP327_001627"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="LP327_001627"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBL1725744.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="LP327_001628"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="LP327_001628"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBL1725745.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="LP327_001629"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="LP327_001629"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBL1725746.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="LP327_001630"
     CDS             21905..22483
                     /locus_tag="LP327_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL1725747.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="LP327_001631"
     CDS             22535..23017
                     /locus_tag="LP327_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBL1725748.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="LP327_001632"
     CDS             23134..23508
                     /locus_tag="LP327_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725749.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7683 a         4250 c         5044 g         6548 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat ttttttaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaa
//
LOCUS       DAFEHS010000040        23429 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFEHS010000040
VERSION     DAFEHS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.041"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(7..2151)
                     /locus_tag="LP327_001633"
     CDS             complement(7..2151)
                     /locus_tag="LP327_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL1725750.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2508..3881)
                     /locus_tag="LP327_001634"
     CDS             complement(2508..3881)
                     /locus_tag="LP327_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL1725751.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPIMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3881..4276)
                     /gene="rbsD"
                     /locus_tag="LP327_001635"
     CDS             complement(3881..4276)
                     /gene="rbsD"
                     /locus_tag="LP327_001635"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBL1725752.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGSEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4304..5221)
                     /gene="rbsK"
                     /locus_tag="LP327_001636"
     CDS             complement(4304..5221)
                     /gene="rbsK"
                     /locus_tag="LP327_001636"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBL1725753.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5330..6343)
                     /locus_tag="LP327_001637"
     CDS             complement(5330..6343)
                     /locus_tag="LP327_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL1725754.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6424..7026)
                     /locus_tag="LP327_001638"
     CDS             complement(6424..7026)
                     /locus_tag="LP327_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL1725755.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7211..7912
                     /locus_tag="LP327_001639"
     CDS             7211..7912
                     /locus_tag="LP327_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725756.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7922..10633
                     /locus_tag="LP327_001640"
     CDS             7922..10633
                     /locus_tag="LP327_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1725757.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11030..12658
                     /gene="treC"
                     /locus_tag="LP327_001641"
     CDS             11030..12658
                     /gene="treC"
                     /locus_tag="LP327_001641"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBL1725758.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12718..13899)
                     /locus_tag="LP327_001642"
     CDS             complement(12718..13899)
                     /locus_tag="LP327_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL1725759.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14151..15677
                     /locus_tag="LP327_001643"
     CDS             14151..15677
                     /locus_tag="LP327_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL1725760.1"
                     /translation="MKRLIGILAMLVLAGMLTACGGASGKAEDSKEKLSVMTTFYPMY
                     DFTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQK
                     TLQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVAN
                     IRDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLAL
                     EYGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGV
                     SLEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNG
                     YFEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGY
                     QTDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGA
                     FRYIQFSDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH
                     "
     gene            complement(15746..16474)
                     /locus_tag="LP327_001644"
     CDS             complement(15746..16474)
                     /locus_tag="LP327_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1725761.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16487..17767)
                     /locus_tag="LP327_001645"
     CDS             complement(16487..17767)
                     /locus_tag="LP327_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL1725762.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYAQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17908..18510)
                     /locus_tag="LP327_001646"
     CDS             complement(17908..18510)
                     /locus_tag="LP327_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL1725763.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18756..18962
                     /locus_tag="LP327_001647"
     CDS             18756..18962
                     /locus_tag="LP327_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725764.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19275..19994
                     /locus_tag="LP327_001648"
     CDS             19275..19994
                     /locus_tag="LP327_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL1725765.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20075..20758)
                     /locus_tag="LP327_001649"
     CDS             complement(20075..20758)
                     /locus_tag="LP327_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBL1725766.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            20990..23176
                     /locus_tag="LP327_001650"
     CDS             20990..23176
                     /locus_tag="LP327_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL1725767.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7812 a         4208 c         4582 g         6827 t
ORIGIN      
        1 tttattctat ttctttaaag gcagatcaag ccagaatgat ttgtctaaac ttaacaaggt
       61 tagtacagga acagttgcag ctttaactgt acgagtagtt gtttctggta tctgtcttgc
      121 cgtaaaattg ttccacaaat cccagattga agcgacacgg aagatggaag aagcgttgtt
      181 gacgcgtttt atcgttgaaa attctaatcc tgtgattggc acgccatctg ttgtccattt
      241 gattggcaca tcgaatcgct tagacatatt gaggatttca gcgtttgctt cttcagttgt
      301 tgctgcttct tttttcggtt catcatattt tgtcaggtcg tacgcttcga tgatcgcgtt
      361 caatttttgt gaatattggc gatctgtagc ataacgtcct tccaaatatt tggttgcttc
      421 tttgtagtct tttgtttccg acttccaagt tggtttgtag aaatcaggat tgccagtcag
      481 tccatttttg atcaagtcgg catagtcttc caaactttcc ttgtaagaag gatatttacg
      541 gaattgtgcg cggatttggt agctttgtcc ggaactatct tgttcaagtg tattgaaggt
      601 cacgctgctt cctttatatg atcctttgat accaaaaagg ttatgatttg gttcgcttga
      661 taataagctg tttccagatc cactttcaag gatagcttga gccatcatga ctgatgcgta
      721 gattccttct ttttgcccga tttcctgtgc atcatctgca atctttttca agaactgatc
      781 agttgtttgg ttttgactga aatggaacga gccatctaat ccatctaaaa tagatgagcc
      841 gcctgtcagc gaacctgaag cagcaggtaa acctgctaag tttactcgga cagcaaaatc
      901 aggcgtgaaa tctgaaatag aagttactcg aactttatct cctggttgag gcgcatggat
      961 aaattgtccg ttcccaatat agatacctac gtggtgggta acaccagaag aaggtcccca
     1021 gaagacgaga tcaccaggtt gggcctgact tactggaatc tgtgtacctg catattgctg
     1081 ttcgcctgtc cagccaccga tataatgacc ggttgctttc atatatacgt aataagtcaa
     1141 accagaacag tcaaaagtat ttggaccttt tgctccccat acatatggtt ttccaagatg
     1201 cttgaatgct tctcgaacga ttgcttgttg gacattcgtt gtatttgtag gcaatacagc
     1261 atcaattggt gattttacaa caaaatcaga agaagcagaa gatggattct ctataccgct
     1321 ttgatttgct gtatttggac gagatgaagc attgttcgtt ccgccatttg atgaaccatt
     1381 gtttggatgg tttggttctc tctgagtatt tccattgtta ttcgatccag atgggtttgt
     1441 ttgattcgaa gaattcccgc tgttcgaatt attgttatta ttgtttgaat tatttgaacc
     1501 attattattg ttgttattac ccacatctgg tttgtttggc gtttccggat ctggtgtact
     1561 agggacttct tcactaggat cagttggttt ctctgttgaa gtatccggta caggttcaaa
     1621 ttctgcagta atagataaat tgttcgtacc tactgtaaag gtctttgcat cataatttac
     1681 ttctttggca ccattaacag cgactgaagc aatttgttca ccttcagctg gcgtaatagt
     1741 gtaagttacc ttcgtttccg ctaataaacc ttctaccgtt ccactttccg taatatccaa
     1801 aggttgctgt tcaggggaat ccgctgtcga agcagtcagt tggatcttac caatcaaaga
     1861 aggagcaatt gtaagggaat atgtctcggc tggcttttca ttttctgatg gatcagccgg
     1921 atcaatttca gttggctcgg tcgatcctgt tgttgagtta gttgttccag ttgggtcttc
     1981 tgtaaaagaa tccgttgttt cactagtagc tgagctggat gtagtcgtat ctgttgtgac
     2041 tgtttgatca gctgtttgat tatctacggc aaatacggta ttggtcgaca aaacagacgg
     2101 agcaatcagc caagtagcag ctagcaaacg tatcgttttt ttatttttca taataaacta
     2161 gtccttttcc tttcattgat tttctgtctt attcagcatt cattagacat tttttggaac
     2221 agataatcgt gtcgaaaagc tttttctttt cataaatctg tctgtgtatt ctaaataaaa
     2281 caatttgtaa ataagcaacg agcattcatt cgtttttcat tgacgttcat tttattgcat
     2341 agaccacctt tgtttgggga taaacaagtg gggataaaaa tggttatgca tatgtaaatt
     2401 ataccaaagt ttggaaagta tcacaaaaat ttataaaaat aatgatccta aaaagaaaaa
     2461 gactgaaata tgtgataaac atctcatact tcagtctttt tcattaacta ttcataagac
     2521 attaggtctt catcaaaccc gctaacgaga gtagtaatca ttgctacgac tagactaaca
     2581 gccagtcctg ctaagtacca gaaaaaagta ccggaaatga atgcaggcaa tgtggtcaga
     2641 ctaccaaata caaaagcgat cgctttcact tgggcaaatc ccattacagc tccaccagca
     2701 gctgcaccaa tgcaagcagc aacaaatggt tttttcaatg ggatcgcaat tccgtaaaca
     2761 gttggttcgg tcactcccgc aagtaaaatc gaagcaaaag cagaaccgga gatcgaacga
     2821 gtttttgaac gtttacttcg tagaaataca cctaaagctg caccagccat tccaaaattg
     2881 gctgctcctg tcaacggaac gattcggtcg aacccataag cagagatatt actgatcatg
     2941 atagggttta cagcccattg gataccaaat attaccaaaa tattccaacc accagcgatg
     3001 attgcgccgc tgatcggtcc gttaatatcc atcatccaag taattgcggc agctaaacct
     3061 tcgccaaggt aaacacccgc aggcccgaat accattaacg tcaaaggaac cgtgataact
     3121 aatgaaagta aaggtacaaa tactaattgc atattttcgc gaatatattt tttgaggaat
     3181 ttttcaacaa aagaataaaa gaaaatacct aaaatcgccg gcatgacagt agacgtatac
     3241 tgcatcagta ctacaggaat ccccagaaaa tcagtagctg ctttgccact gtctgaaagt
     3301 aaagaggtca gattcggttc aatcagtgct gcagcaatga cagccgcaat atatccattc
     3361 actttaaacg ttttggcggc agaaaaagca agaaagattg gcagaaaata aaagacggca
     3421 ttcgacgcag cagacaaaat atgataagtt ccgcttgttt cactaagcca gccaatcgta
     3481 gtcgctaaaa caaccagacc ttttaatata ccagaaccgg caaacagcgg aatgatcggc
     3541 gtaaagattt ccgatatcaa accaaatagt ttttcgatcg gatttagctt ttctttagga
     3601 gctgaatgat cgatttgacg cgttaattcc ttgtaagcag gagccaatcc ctgatccatg
     3661 attatttctg catatctatc gagtaaaatg aaatacttaa tggaagggat ttgctgtttt
     3721 aatttttctg cattggctac tggctgtttt ttcaatttga tacgaagccg agtcgcacat
     3781 ggaaggacag aatcaatatt gtcttttcct cccaactgtt caatcagcat atgaatctgc
     3841 tcggacagta ccatctgttc aggattcatt gtcgtttcca ttagaagacc actccactca
     3901 ctaatataat attggcgtaa ggtgtcattt cgcctgtacg aataaatgca tgacagttat
     3961 tcaactcttt tttcatttct tcatgtggga taaagctaat cggtgtatct ggtaacaata
     4021 atttgatttt ttccaatatt tccgggttca ttgtctttat ttcttccgcc aattcaatcg
     4081 attgtacctc aagttcttct aaaacgtttt ctaaaacaga taaaaagctt ggaagaccgt
     4141 ttgagacagc caaatctatt ttttcactac caaaggggac aggcatgcct gcatccccga
     4201 tacttaattt atcaaagtgc cccatttgtg cgattactcg tgaaatatct gaattgatta
     4261 cttttccttt tttcatgttt gtttcttcct ctcattgtta attttagact gctgattgtt
     4321 ctaaagcttc gcggtaaggg atagaaggtt gcgcaccata agactgcaca gcaacagaag
     4381 aggctagatt tccataaagg atcgcttctt tcaagttact aaggtctggc tgaagtgtgc
     4441 tgcttaatgc gccaataaat gtatcacctg cggctgttgt gtcaacggca tccactttaa
     4501 aagcagggac gataccgtgt tctttttctg tatgatagaa agcgccggca ctgcctagtg
     4561 taatgattac tgttccaata cctaattcat ggagtttttc ggctgctgcg accaaactgt
     4621 tatgatctgt cactctgatt cctgtaatga tttctgtttc tgtttcattt ggtataatga
     4681 tatctgtttt ttcaagtagt tcagtaggga tcgtttcacg agctggtgca ggattcagga
     4741 tcgttgtttt tcctgcgtct tttgcaatag aaaaagctag gatcgtcgca tctaagctac
     4801 tttcaaactg agcaataata aagtcactgt ttgtaatcag ttgcttgttt tttaggactt
     4861 cttgttcatg gaaagcatta tttgcacctg catggatcaa gatactattt tctcctgctg
     4921 cgtctaccat aatcattgct tgtccggttg tggcgccttg gatttcagct atcccagatg
     4981 tatcgatgtt ttcttttgtc agtagatcta ataactgttg tccttcgctg tcagccccaa
     5041 cgcctccaat aaaagatgtc cgtgcgcctg aacgtttggc tgccactgct tgattggcac
     5101 ctttacctcc gccagcgtga aaaatctcat gcgcatgcat tgtttcgcct ggcttgggca
     5161 tgttggttaa acgaagggta gtgtccatat taatacttcc aattactgta accgtgttca
     5221 ttttacaaac ctccaactat ctcatttatt ttgatgggaa aaattatttt ttaaaacgtt
     5281 ttagtaaaac gttttaatag ttcgataaaa atatagcaca ccttgaaagc tatttcaagg
     5341 gtgcagttga aaatcttttt tctaatcgta caggtaatct tttttcttcc cattcttttt
     5401 ctgggaattg gatccgatct aacaataatt tggcagatgt ttgtcccaat tcgaagatcg
     5461 gttgtgcgat cgttgtcagt ttcggcttga tatactcgca catatctata ttatcatagc
     5521 caataatgct gtagtcttca ggaatgcttt ttccagattc ctctaatccg cgatataatc
     5581 caaaagctaa ttcatcattt aaagcaaaca caccagttgc agcagaatca agaagttctg
     5641 ctgtgatttg gtagccacct tgttttgaga actgtgtagg taagaggatc agttggtcgt
     5701 gagagatttg gtaaacatca agagcagatt taaacccctc aatccgagcg tggacatttt
     5761 ccggcggatt ttcaggatag acaagggcga tcgtttgatg acctagacta agcaagtgca
     5821 tgcccgctag gtaaccaccg cgaaaatcgt ctgttcgtac ggcatcacta aatccttcag
     5881 attttttctg atccaaaaca atgaagggcc ggccttgttt tttcaaattt tcgttgattg
     5941 catctgtaga gacagcagaa gtcgcgataa tgaatccatc cacacctcga cgtgtgagct
     6001 cggctagata ttcgatttct ttttgatgat cagaatcagc attacataat atcgtgacga
     6061 aattctgctt atacaagata tcctcaattc ctcgcatcag tgtactgaag aatggattcg
     6121 tgatatcagg aacgagtaca ccaattgttt tcgtttcctt ggtaatcatt tgtctagcaa
     6181 aatagtcagg ctgatagcct aattcttctt tggcagctaa aactttctga acagttttcg
     6241 gactgaattt ctccgtattt ccatttaata tcaaggagac agttgtgatt gaaacgccgg
     6301 aaagtttagc gacttctttg attgtgatct tttttttcac catgttcata ctcctatcta
     6361 aatcctgtgg ttattttccc atgaaatcaa aaagaagtaa atgaaaatca cgtgtgccag
     6421 tacttatttg ctttgttgga tagcttcgat tatttgtttc gttgagcgta ctcgtgcagt
     6481 tttcggaaat atctttttta tgaacaattg atgggattct ttgtcacgat cactgcaagc
     6541 atcttcagca agtacaacat gatacccatg ttggaaggca tctaatgcag tagcatatac
     6601 accgtttgtt gtcgctacac cgcttaaaac gatagtttcg attccacgcc ggcgaagttg
     6661 aagatcaaga gaagtaccaa aaaaggcact tggattgtat tttgtgatag tcaacatatt
     6721 gtttgtatct ttcaaggaat ccttcaacaa caagtcagtg aattctgccg gagcagacaa
     6781 agcagatatt cctccagcat ctgtctgatg acttagataa gagaaagtag tgatgtctac
     6841 attcactaaa actttcaagg cagcggtacg tttcaatgtt tttgctaaca aatcattgtt
     6901 tgtcagaata gattgtgcag tgtgtggaga gagtgtaccg ttatttacga tccctttttg
     6961 catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt cctttaaatc
     7021 attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat cataaatcgt
     7081 ttatttgtca actaaataaa ggattccttt ctttagtcta gagaaattca gtggtaaaat
     7141 aaataagtat gagaaaacgc attcgatgca tagatatagc gagaaatatc gtgaaaatgg
     7201 aggaatcaga gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca aaatgggaga
     7261 aacgaccatt acagcaaatg acgatatttc tttttcaatt gaaaaaggcg aattagtgat
     7321 tatacttggt ccaagtggag caggaaagtc aacgattctt aatattttag gtgggatgga
     7381 tacacctgat gaaggtcaga tcattattga tgacacagat attgcgcaat tttcagataa
     7441 acagcttact gcttaccgac gaacagatgt agggttcgtt ttccaattct acaatctcgt
     7501 tcctaatcta acagcaaaag aaaatgtgga attggcaacg gaagtttctc ctgatgcgct
     7561 tgatcctgta gaggtattga ggcaagtggg cctagctcat cgattgaata actttccgtc
     7621 ccaattatca ggaggcgaac agcagagggt ttcaattgct cgtgcactag ctaaaaatcc
     7681 caaacttctt ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg gcaagcaagt
     7741 attgaaatta ttgcaaaacg ctagcagaca acacggcaat accgttttga tcattacaca
     7801 taattctgct ttagcgccca tcgctgatag agtcattcac attaatgatg ctaaagtaag
     7861 gtctgttgaa ttaaatgatc atccttcatc cattgatgag attgtatggt agggggattt
     7921 tatgaaaaca agaacatatc ttaaagcagg ttttcgcgaa attcggcaat ctaaaggacg
     7981 attcattgca atcatcctta ttgtcatgct aggaacactt ttatttgtag gtgtcaaaac
     8041 tgctggacct gtcatgcaaa aaacgatgga ccactatgtt ggtacagcag gactttctga
     8101 tttgcaaatc gtttctactg gtggcttgac acaaaaagat attgctcaag cagaaaagat
     8161 cccagatgca aaagtagaaa caggaaaaca gatctattat tccaatactg gtaaaaacga
     8221 agtgattcaa atattttctt ataacaaaaa taacaagcag aacaagctac aattaactga
     8281 tggaaaactc ccggaaaaac caaaccagct tgtattggat gaaaaagcgg aagatgaagg
     8341 gtataaaatc ggtgatattt atcagattga ttcagatgaa ttgaaagaaa aagagtatac
     8401 catcacgggc tttgtccgtt ctccattgtt catcaacaat ttggagagag gatacgcgaa
     8461 cgtcggaaat ggtagcgtcg attattttgt ctatttacct gaatccgttt ttaaaacaga
     8521 tatttattct gtcttatatc ttgattttac aaacgtaaag aatttgaata cgtacagcaa
     8581 agcttacaaa gataaaatgg aacacaatca agagaaggca gaaaagtatt tacaagatcg
     8641 ccctgaagaa cgcttagaag agctgaaaaa atcggcaaac gaagaattag aaccagcaca
     8701 gcaaaaaata acagaaggca aagcacaaat agcacaggga agaacacagt tagaagatgc
     8761 taaaaaacaa ttagcgcaac agtcagcagc tatccagcag cttcctgaag cgcaacgaac
     8821 aatcgctgtt caaacattat ctcaacaaga agctcaactt gctgaacaag aaaggcaatt
     8881 agttgagaaa gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa atgcagaaaa
     8941 aattgaaaat ttaactaaac catcatacct atacaatgaa cgttcagaaa atgtcggttt
     9001 tcaagaattt ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt ttcctgtttt
     9061 ctttttcttt attgccgcat tgatcacctt tacgacaatg acacgtatgg tagaagaaaa
     9121 ccgcagggaa atcgggacat taaaggcgtt aggttatacg aaagttgaaa tcgcaaaaaa
     9181 atatgcaatc tatgcaagtc tagcctcagg gataggcatt gttttaggaa cgatattagg
     9241 aacgaatctt ttgccaagga tcatttttga gctgtctaac gagcggtatg atattgggag
     9301 tgcgcttatt ttttatgact ggccgccgat tttacaagca gctgccgcat tcttcattgc
     9361 cgcatttggc gctgcaatga tcgttttatt caaagattta agagaacggc ctgctgcatt
     9421 gcttcaaccg aaagcaccga agccagggaa acgcattttt cttgaataca tcactccgct
     9481 gtggtcacgc cttagcttta atcaaaaaat cagttaccga aatctcttcc gatataagtc
     9541 aagaatgctt atggcgatta tcggaattgc tggatgtgcc gggctgatgg ttgcaggcgt
     9601 gggattgaaa gattctctaa gttctgtctc agctaaacaa tttggaccga tcattgacta
     9661 tcaagcaatc gttacattgg attcatccac tgaagatagt cgtcaaaaag cagcggatat
     9721 gctgcagaag caagaaaaag taaatgagat cctagctatt catactcaaa ccgtagaatt
     9781 gagaaaaaaa ggacatgctt cgcaaagtct gacagtgatc gtaccaagta aaacagctga
     9841 tttggatcgt tttatccatt taactgatct tgaagagaac ccacgctcct tgcctaaaac
     9901 aggaatcgct attacgcaaa aagcagcaga actatttgac ttatctacag gagatcatat
     9961 ttcgctttat gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa tcgtgaaaaa
    10021 ctatctaggt agctttattt atatgtcccc tgaatattat gcttcagtta ctgatcaatc
    10081 gatgatggat aatgcatttt tgctgcgtac agataagatg acaacaaaag aagaagaaat
    10141 gctttccgag aaactgcttg ccacagacgc tgtgacaaat acgtctttca tgtccaaaca
    10201 aatcgaaacg caagaagaat caatggcgaa cttagactcg gttgtcttaa tctttgtcgt
    10261 attatcagga ctgctggctt ttatcgtctt gtataatcta actaatatca atatttccga
    10321 gcgtgtacgc gaattatcta caataaaagt ccttggattt tttgataaag aagtcacgat
    10381 gtacattgtt cgagaaaata tcatctttac tcttttagga atcatcggag gatttggtgt
    10441 aggatatata ttgactgact tcatcttgca acaagcgtct atggagaatg tcatctttcc
    10501 attagtgatc acatggtggt cttatgtctt atcagcagga ttaactatcg tatttacggt
    10561 tattgtgatg attgttaccc atttcaaatt aaagcatatc gatatgattg atgcgttgaa
    10621 atcgaatgaa taaaaacaga ggttgtgaaa tcagcgtttt gagctaataa cgagggtcag
    10681 ctgctaattg aacaccgtga atcagaggtt gtgaaaaagc tgtcctgcat cgagcaaaag
    10741 gaatactcaa ataaaattag ctcctcatgt tttacatttg agaagactga ttgccgtaga
    10801 taaaagcttt tgagcaccat ttatagaaga aacaagaata ttttgctatt ttaaacaaga
    10861 ggatagacaa atacatgtct gatctcttgt tttttattgg gcaacagacc tcttagaatg
    10921 tataaagata aattttacaa acgctttctg ttcagttata ataataaatg ataacttgta
    10981 tatataagtt gcggtaggta ataaatcagt tgaatggagg taaatatcca tgacatttaa
    11041 agacaaagtc atttaccaaa tctatcccaa atcttacaaa gacagtaatg gtgatggaat
    11101 tggagattta aaaggaatca aagaaaaact cccgtatctg aatgatctag gcatcgacat
    11161 gatttggcta aaccctattt atccaagccc tcaaaaagac aacggttatg atatctctga
    11221 ttacacagcc atcgatccta tctttggtac gatggaggaa ttcgaacagc taatagccga
    11281 agcaaagaaa taccggatag aaatcatgct cgatatggtg ctgaatcacg tttcgacaga
    11341 acatgaatgg ttcaaaaaag cactagcagg ggaccagcat tatcaggact tcttcatttt
    11401 aagagaagag ccaacggatt gggtatcaaa atttggtgga agtgcttggt cgccatttgg
    11461 agatacgggg aaatattatt tgcacttata tgatcggaca caagctgact tgaactggcg
    11521 gaaccaagca gtaagagagg aactattcaa agttgttcgt ttttggatgg aaaaaggggt
    11581 aaaaggattc cgtttcgatg ttatcaatgt tatcggtaaa gacgaacaat tgaaaaataa
    11641 caaagaaaat gatggcaaac ctgaatatac ggacaaacca atcacccata cgtatctaaa
    11701 agaattgaac caagcaacgt ttgggacgga ttccactatc atcacagtcg gtgaaatgag
    11761 ctctacgacg atcgaaaatt gtatcttgta tacagaacct agtagagaag aattatcaat
    11821 ggtctttaat ttccatcatt tgaaagtcga ttatgaaaat gggaataaat ggtctactcc
    11881 tccatttgat tttgcagaat taaagcgact atttcataca tggggagaaa agatgagtga
    11941 gggtaacggc tggaatgctc tattttttaa taatcatgat cagccacgtg cgctcaatcg
    12001 atttgtagct atcgatcagt atcgaaaaca aggagcagag atgctagcaa ccatgatcca
    12061 tttgaaccgt gggacgccct atatttatat gggagaagaa atcggcatga tcgacccaga
    12121 ctattcatcg atttccgact atattgacgt agaaagtttg aatgcttata agttactcct
    12181 tgaacaagga ttcacagaaa aagaagcgtt tcgtcggatc aaagcaaaat ctcgcgataa
    12241 ctctcgaaca cccatgcaat ggtcagacac agaacaagca ggatttacta cagggaaacc
    12301 ttggttaaaa gttggaggca agctagaaga gattaatgta gaaaaagaac tatcttctga
    12361 agattctatt ttgtcctatt acaagaaatt gattcggctg agaaaaacat atccaatcgt
    12421 tgcacaagga gattatcatg cttatgaagc aaatcatccg gaagtttatg gctttcttcg
    12481 ccaatttgaa ggacagcagc tgcttgtatt aacaaatttc tatgataaag atacaaggat
    12541 caccatccca gaaggattca cagaagccaa tattcttatc gataattatt ccgatactgt
    12601 tcttcatcaa agtctggagc taaaaccata ccaggcaatt gctttgctga aaaattaaaa
    12661 caaaaacaca aaacgtcgtt cctacgagct attctcgtaa gatcgacgtt ttgtgtttta
    12721 tttaaaataa tcttcttcag aaaatgaaga gttttcatac tctcctttat cgccataaaa
    12781 tttattgtag cgttgtttga atttacggaa aataaaagta acgagtaatt tgactaacgt
    12841 atagatagga ataccaaaaa taacgcccac aattcccatt agatcaccca tgacaagtaa
    12901 aacgattaag atcgtaatcg gatggatctg taaacgatca cccattactc gaggaatcac
    12961 caagtcaccg tggagaagtt gtacggcaaa ccagacaatg acaaatttga cagccataaa
    13021 agtagagtct tggaatgcaa tgatcaaacc tggaaagaag gcgataaacg gcccaatata
    13081 tggaatgata cataacaacc cagcagaaat agctaaaaca ctagcaaatt ctaatccgat
    13141 cagtaagaaa caaacccagt agactgctcc aaggataaat gaagcaatga tttgcccctt
    13201 tagaaatgaa cccaattgtt gattagcaat ctttgtcaaa tttttaaaat ctgtacgaaa
    13261 tgctggtggg acaatcgcta aaactgactg ataaaatttt tgaggatctc tcaaaagaaa
    13321 aaaagcgaca attggaccag taaataaagt aataaaaata gctgtaacag ttgaaaaaat
    13381 gttgctgatc cgttgtgcgc cactttccca ataatttcca ataaatccag ctatatcaga
    13441 cgtcacgtcg tcaatagaag cgaaaaattg attaaatgaa tcggcaaaag gtgttttctc
    13501 taaaaattgt cggagactct cttgaaaatc ttgaaacaaa tctggcaaac tactgatcaa
    13561 tgtctttgcc tgttcttcaa gaaaaggaac aagccatacg cttccaagcc ccaataaaaa
    13621 taaaataatc acataaacga taatgactga caagttcctc gaaaggtgct tgctcaaacg
    13681 tttgatgact ggttccaaaa gataatacaa gatcagtgca aacaataatg ggggcaagat
    13741 agtagcgata atgattccga caggcttgaa tataaacgag atctgatgga aaaaataaat
    13801 gatcagcgca agtaagataa ctattcctaa aacatagtaa gaggtttttc ctcctaaaaa
    13861 gcggatcaaa cgattcgttg tatctgtttc ttttttcact ctttttcctc ctttatctat
    13921 aacttcatta tagacaaaag cgggtataaa aggaaaatga tagaggttga ttgcaaaaat
    13981 atcgtcaatc attcatataa actttctcat caattaaaat aagaaaacgc ttgacagttc
    14041 ttttaaccat atgataaatt aaatcctgtt gctaaaacgt aactattacg ttttgtaaaa
    14101 ggcgaatcgg aacattcaaa tgaaagaaga tgagaagagg agtgtcataa atgaaacgat
    14161 tgataggtat attggccatg ctggttttag caggtatgtt aactgcttgc ggcggggcaa
    14221 gtgggaaagc cgaggacagt aaagaaaaat tgagcgtgat gacgaccttt tatcctatgt
    14281 atgattttac aaaagcaatc gtcggggatg aaggagaagt agagttattg attccagcag
    14341 ggactgattc ccacgattat gaaccttctg ctaaagacat ggcaaagatc caagatacag
    14401 atatctttgt ttataatgat gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat
    14461 tacaagaagg caatgtccat acgataaaag cgacagaagg tatgctttta cttccgggaa
    14521 gtgaagaagg acatgatcat gaccatgaac acggggaaga aggccataca catgagctag
    14581 acccacatgt atggctagct ccaagtttag caatcaagca ggtagcaaac atacgtgatc
    14641 agctgattga agcttaccca gaaaaacagg aagtatggac aaaaaatgca gcagcctata
    14701 ccgagaaact tcaagcactc catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa
    14761 gatcttttgt cacgcaacat gctgcattca actatctggc attagaatat ggattgaatc
    14821 aagtatcgat tgctggtctt tcttctagtg aagaaccgtc tgcggcgaga attgctgaac
    14881 tgaaacactt tgttaaagaa catggaatca actatattta ttttgaagaa aacgccaaag
    14941 atagtattgc cagaacgtta gcaaacgaag caggtgtatc tttagaagta ttgaatcctt
    15001 tggaaggatt gacgaatgaa caaatagaaa atggagaaaa ctatctttct atcatggaag
    15061 caaacctcga agcgttgaaa aaaacgacag agacagaaaa cccgctagaa gatactttga
    15121 cccccaaaaa agaaaagagc gtctacaacg gttattttga agacgaggat gtacaagatc
    15181 gctctctttc agattgggca ggaacttggc aaacagtaga tacgtatgta gaagatggga
    15241 cttttgatcc tgtttttgaa tataaagaaa aattgaatca ggataagaca gcacaagaat
    15301 ataaagaata ttatacgaag ggctatcaaa cagatattgc ttcaatcaag atcagtgaga
    15361 aagagatggc atttacgttt aaggatggaa caacgaaacg ttcaaattat cgttatgtag
    15421 ggaaagaaat cttgacctac gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag
    15481 aagataagga aagtggcgct tttagatata ttcaattcag tgatcacacg attgcaccgg
    15541 ctgactccga acattttcat ttatatgcag gaaatgaaag tcaagaagca ttgctgaaag
    15601 aaatggagaa ttggccaaca ttttatccag atacaatgga tggacgtgaa attgcacagg
    15661 aaatgctttt gcactgatag gcaaaaaaga accgaagggt ttggatcggc tagtcattta
    15721 gtcggtccaa actattcggt tctttttatg aaatcaattt ctttaatcca cggatagagg
    15781 ctgggatggt ctcgccgttt gccaacgtga cttctctggt ttttttatta atcgaccgca
    15841 cattttgtgt attgataaca taggatttat gacaacggat aaaattccga tgcattttct
    15901 cgatttcttt gatcttctca tagaattgta attgggcatg agtcaaatgg acttccagtt
    15961 tatgtggtgc tcctagcgaa gtagaaaaat attggatgtc tttgatcggt aaaaatttga
    16021 tctcatcatt aaactctatt ttgaagatat ctttttggat ctgggacatt tgaaaatctt
    16081 cgaaaatttt gctcaggata tgctcgacat tttcttttaa atcataaaga tcttctttga
    16141 cgatataatc agtcggttcg atgttgcttt ttaagatttt catcgacatt tccgtatggg
    16201 aagtcacata gattattttt cctaagggat ctaattcacg gatcaattta ccgatcgcta
    16261 caccatccat ttcactgtct tctagttcga tatctaaaaa gtaaatacca aaagaagaag
    16321 ttctgatctt ggctgcattg agaagttctt ccggatcaga ggtagaaata tggacataac
    16381 tgtctaagtt ttcaatcatg attctatttt tgatcaccgt tgataaaact tctaattgtt
    16441 ttggatcgtc ttcacaaata taaatgggaa tcatatcatt actcctttat tttatttgta
    16501 tataaatcgt ttgagaaaaa cgctggtttt caattgatgt ttcaaggaaa agataaggat
    16561 tggtctttgt caattcatcc aagataaaca agccaaggcc ttcgtttttt ggatttgttt
    16621 ttgttgagaa ccctttttgt ttcaactggt ttaacggcaa tgtatttgtt gttgtattga
    16681 tcacacgaat catcacggaa tgattcttct tgagtatggt caattcgatt tttttcttct
    16741 ttgcagcact agctccttca atcgcattgt ccaagataat accaagcata cgtacaaggt
    16801 ttaccgtatg tttcgtatcc agttcgattg gctcaggaaa atgaagctgg acgaccaacc
    16861 ctttttcctg tgccatcatg agttttaatg acagtaaact acggatctca ggaactttca
    16921 tattatccaa catacttaga ttggccggaa aaaggttcat catctggcgt gtaggtaata
    16981 tcgtttgagc ataataagac cgtaatcctt ctaagtcgcc ttctgaaata tagctgtgaa
    17041 gagaaaaaag cagattttgg taatcatgtt tgaattctct caagtcttga tagttttttt
    17101 ctagatcttt gatataatcc tgttgcatcc tgacttgttg ctctaataac tgatcagcta
    17161 atttcttatt agagattttc caataataaa ccaagatgat caggccaaca cacagatata
    17221 gaatgaaaaa actcgtattc aaagctaaga catcaggtgt ttcaccagaa aaacgggtat
    17281 aaaaaataat gatatagtat gtaaataata tcaatgtccc gatcgtaccg atcatacggc
    17341 ggtccacagt gtgaaaccaa gtctctaacg attttttaaa acaaaaagct aataaagcag
    17401 ataaaaaagc ggataaggca atgtgaaaat actgcaatga atctcctggt tcaataaccg
    17461 aagatttcaa aacaaaaaaa tcgccgatgc ttgttaagtg atctcctaaa agcgacgtca
    17521 ataacccaaa aggaagcgcg taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag
    17581 taagcatcag cattcctata gcaattagag cagtaaaaat ccctaaaatc gaatagaccc
    17641 atccaatcaa aatacaagaa atcagaatga ctcccgcaaa cagatatttg tatcggatgt
    17701 gatggtcaat caataagata caaattgtgt aaatcaaata ttgcaacgct atggaccata
    17761 aaaacataaa gtacagctcc cattgatttt taccaaatta tagcacaatt agcgtattat
    17821 ttttttaatt tttcagggat tttgaattca gaactcatac caaagcagca gccagataca
    17881 aaaaagtcag ataagttagt taattgttta atgattgttt ttttcatgaa agattcctcc
    17941 aaaattcatc aagtagtttg taataggaag caacgtagct atttctaaaa caatcccaag
    18001 tagcaaatag gtagaaaagt aagaagctga gaaaaataaa gtaatgagag aaaaaattag
    18061 aaacatgctg atcgccttct ttaataatgg gggacgtttc tcgggttcaa tgggattgat
    18121 tgctgtacct cttgatccat agcgaagcag taacaaaaag cagctgatta acaaaaagac
    18181 catcattggg aaggagatag tcaattgcag atgtttcatc accaagggaa cgccccataa
    18241 ataaagtagt gtccacagca gacaaacgaa ttcagagtcg gaatgcaatc cataagcata
    18301 tctgcggatg catatataag aaagaaaggt gattcctgtt tccgcccatg agcctgtcac
    18361 cagagaaaag actagaacga ccgctagttt tccaagatta ttcaataaaa cttctaaggc
    18421 aaattttact tggacataca ccatatcttc ttccgatttg tctccgctga ataaggcttc
    18481 taataatttg ttcacaactt tatctttcat ctcatacgta cctctaccac aaattttatg
    18541 ctaaaaaaaa taaaaaacgt cttttgtttc ataaaaagtg gttttgacat caaaaccgtc
    18601 atgatttcct aaaaaaatct tcgttttcca agaaccttac caaaaaaagt gacagttttg
    18661 atacgaaaag tactttttat gacaagtttg tgctttttcg agtgatttat gttaaaaaaa
    18721 ttactgacct tataaaaggt gatggaggga ttcttatgga aaatgaacaa ttgtttgaag
    18781 tcggcgaaac atatgcattt aaatctgatt tatttgaatc accaataaaa ggaaaaatag
    18841 aaaaaatcta tgaaaatagt gtgatgatac gtgtcatgaa atgcgcagta gaagacaatg
    18901 ataccgctca taatctgcac tggcagttag ttgtacgcaa atcaagtaca atagaagaat
    18961 aaaaacagat aaaatgttga gagcataaag aggttgtgag tctccgttta gctctgagtt
    19021 acaaggaata atgttaaaaa acagcttttc atgttttatg aatattgcca cttgtgaccg
    19081 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
    19141 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
    19201 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
    19261 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
    19321 aaagcacact gactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
    19381 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
    19441 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
    19501 acaatgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
    19561 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
    19621 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
    19681 cttttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
    19741 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
    19801 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
    19861 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
    19921 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
    19981 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
    20041 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
    20101 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
    20161 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
    20221 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
    20281 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
    20341 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
    20401 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
    20461 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
    20521 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
    20581 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
    20641 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
    20701 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
    20761 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
    20821 aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt ttagaaaggt
    20881 ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg aaataaccaa
    20941 cttgaaataa acaaattcaa agaagaaaga tgggaaggaa atcaatcagt tgaatgaaaa
    21001 agaaattctc cgattacaaa aagaattgaa acgctgggag aacaagtata aaaagcttca
    21061 acaagtcttt cggctacaag aagaaatcat tgctacctat gaagcggctt atggcgaagc
    21121 acccggactt attgaggaag aagtacaagt aaaagaaagt gaaggaaaca gctctttctt
    21181 taaaatgatg gagaaacgtt cctataatcg cttcaatcaa gaacaaaaag cagctatcac
    21241 atatgatatg aagaaacatc ttcgaatcat agcaggagct gggagcggca aaacgcaaac
    21301 aatctgtgca aaagctgttt atctgatgac gcaaaaacaa gtagacgaag aacgcatact
    21361 aatgatcact tttacaagga acgctgcaaa tgaactaaaa aaacgagtag ataatttcag
    21421 ccagagaaaa accgctgtcc atatcggaac attccatagt atctttttta ggatatacaa
    21481 tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga atacaaggag acttttcaaa
    21541 ggatactgca caaaaagtga atgctgtttt gcaacagctg atacgtaaat ataatcttta
    21601 tatatttgat caatatggag aaaaaacgat tgcttctcgg ttagactatt ggcagaatat
    21661 gaacctttca gtagaagaaa tggttcagtt agttaaagaa aaatatgatt ccattgataa
    21721 gaatgcacga cagccgatat cagaacgact gtatggattg ctcacggaac tccaagaaca
    21781 aaaaagaagc cagcaattgc tagagttcaa tgatgtactc caaaatctga aaagcgcttt
    21841 ggagaatgag gagatccagc attatatcgg ccaaaaatat gattatctat tcattgatga
    21901 atttcaagat actaatccac ttcaatggga aatcgtgaaa cggatgacaa aggaaaacag
    21961 gatcaagttg attgttgttg gagatgatga tcagagtatc tatggattca gaggatcaga
    22021 gccagcatac atcaagaact ttgaaaaaga atatcctacc aaaactcttt tcttattaac
    22081 taattatcgt tctcgggcag ctattgtcca aggagcaaat agattgatca gctataataa
    22141 aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa gaagcaggtg ttatggaagc
    22201 ctatttgttt tctgatacta aagcagaaag tcagtggcta atcaaacaaa ttcagacatt
    22261 tattcaaaaa aatgggaaat ataaggaatc aatcatatta taccgatcag cttcacaaac
    22321 gcagcagctt gtccaatctc tgctgaaaac gaatattcct tttgtactgg aagcagatag
    22381 tccatatgaa ggtatctttg gtatcaagtc tttccaacat ttctatcaaa aaattgttca
    22441 ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc taccagcaat tgctccggca
    22501 gctgatggca gactgctacc tgaaaaaaag tgaatgcgat caatactttt ctcctaaata
    22561 tcaaaatata gccgttgcag attatatttt gtcgataagc cccaatttaa aatcaaaatc
    22621 tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca gaccagcatc aagaaatcta
    22681 ttcgcttgtt caaagctatc ttcatctgcc acgtatagct aaggaaatcg accagagtga
    22741 tcaagaatgg atacgagaag aagtaaagca gcatgctact ttcagaagac tccaagcctt
    22801 atcgcaagaa acccaagcga cagctaaaga attaaaaacc cgcttgattg cttacagaca
    22861 aggaaaactt gatgcactat gtatccagag cattcacaaa agtaaaggtc ttagttaccg
    22921 aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct tataacggag cgacagaaaa
    22981 gaaagtaatc gactttaatg aaataaaagc agaaccagtg acgacgatag aagaagaacg
    23041 aagactattt tatgttgcga tgactagagc aagaaatcgt ctgtatctat gtgtacctca
    23101 aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc aaagaaaccg ggagtcgtgt
    23161 gaaaaaagga aagtgataga agagagggtg tgacagaagt ggtcagtttc aagaaagaag
    23221 aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat ttcagcttat tttcgaagaa
    23281 gctatttctg gaacaccgtt tattcggaaa aaggaggtgt gacaagaatt ttgtcacacc
    23341 tcccttattt attttgaaaa tgagaatact attatcaaaa tagagaatat atagaataat
    23401 tctcattatt caaacgtaaa taatcagtt
//
LOCUS       DAFEHS010000041        23327 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFEHS010000041
VERSION     DAFEHS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.042"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..208)
                     /locus_tag="LP327_001651"
     CDS             complement(<1..208)
                     /locus_tag="LP327_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL1725768.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(307..621)
                     /locus_tag="LP327_001652"
     CDS             complement(307..621)
                     /locus_tag="LP327_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725769.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(689..1183)
                     /locus_tag="LP327_001653"
     CDS             complement(689..1183)
                     /locus_tag="LP327_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725770.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1176..1490)
                     /locus_tag="LP327_001654"
     CDS             complement(1176..1490)
                     /locus_tag="LP327_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725771.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1706..3097)
                     /locus_tag="LP327_001655"
     CDS             complement(1706..3097)
                     /locus_tag="LP327_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL1725772.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3082..3693)
                     /gene="mobC"
                     /locus_tag="LP327_001656"
     CDS             complement(3082..3693)
                     /gene="mobC"
                     /locus_tag="LP327_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBL1725773.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3697..3894)
                     /locus_tag="LP327_001657"
     CDS             complement(3697..3894)
                     /locus_tag="LP327_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725774.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(3885..6563)
                     /locus_tag="LP327_001658"
     CDS             complement(3885..6563)
                     /locus_tag="LP327_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725775.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            complement(6550..6768)
                     /locus_tag="LP327_001659"
     CDS             complement(6550..6768)
                     /locus_tag="LP327_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725776.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(6844..7896)
                     /locus_tag="LP327_001660"
     CDS             complement(6844..7896)
                     /locus_tag="LP327_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL1725777.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(8049..8375)
                     /locus_tag="LP327_001661"
     CDS             complement(8049..8375)
                     /locus_tag="LP327_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725778.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(8387..8587)
                     /locus_tag="LP327_001662"
     CDS             complement(8387..8587)
                     /locus_tag="LP327_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1725779.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            8710..9312
                     /locus_tag="LP327_001663"
     CDS             8710..9312
                     /locus_tag="LP327_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1725780.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            9367..10533
                     /locus_tag="LP327_001664"
     CDS             9367..10533
                     /locus_tag="LP327_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL1725781.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            10950..14177
                     /gene="fss3"
                     /locus_tag="LP327_001665"
     CDS             10950..14177
                     /gene="fss3"
                     /locus_tag="LP327_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL1725782.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVNCTVERHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFEKILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            14290..14604
                     /locus_tag="LP327_001666"
     CDS             14290..14604
                     /locus_tag="LP327_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL1725783.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            14617..14991
                     /locus_tag="LP327_001667"
     CDS             14617..14991
                     /locus_tag="LP327_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL1725784.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            15001..15336
                     /locus_tag="LP327_001668"
     CDS             15001..15336
                     /locus_tag="LP327_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBL1725785.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            15419..16435
                     /locus_tag="LP327_001669"
     CDS             15419..16435
                     /locus_tag="LP327_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL1725786.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFPDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            17029..18903
                     /gene="ltrA"
                     /locus_tag="LP327_001670"
     CDS             17029..18903
                     /gene="ltrA"
                     /locus_tag="LP327_001670"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL1725787.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            18975..19349
                     /locus_tag="LP327_001671"
                     /pseudo
     CDS             18975..19349
                     /locus_tag="LP327_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346898.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            19444..20119
                     /locus_tag="LP327_001672"
                     /pseudo
     CDS             19444..20119
                     /locus_tag="LP327_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            20112..20276
                     /locus_tag="LP327_001673"
     CDS             20112..20276
                     /locus_tag="LP327_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725788.1"
                     /translation="MTDKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            20352..21494
                     /gene="dcm"
                     /locus_tag="LP327_001674"
     CDS             20352..21494
                     /gene="dcm"
                     /locus_tag="LP327_001674"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBL1725789.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AALLRPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYIPQSRERI
                     FLIGHSRDACTEQVFPIIGPSSTSDQNIRNLVNINPSNRGMGGQVYGSDGVAPTLTGD
                     EGIKIALPARDGISVAGLLPGKFEQGNRVYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAIVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVQKCDL"
     gene            21484..21774
                     /locus_tag="LP327_001675"
     CDS             21484..21774
                     /locus_tag="LP327_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725790.1"
                     /translation="MIYDELVGEIYWVIEKIQSDPELEEQLRRLNFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            22032..23216
                     /locus_tag="LP327_001676"
     CDS             22032..23216
                     /locus_tag="LP327_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL1725791.1"
                     /translation="MVQQNLDYRLLKDRRNQYGVSQNKLATACGLSRPYLNQIENGGV
                     TTSTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVQKIVQEILHLKFDYMLHED
                     YAFYSYQEQYIMGDIVVMLSHEEEKGVLLELKGRGCRQFETFLLAQKRSWYDFFDDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDKSETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIEGTTFSIINRYLRFTDKVEGKRRTNWPLNERWGRFIGRNRKEIQLTSDPKPYT
                     IERTLSWLGRQVAPTWKMAKELDRLNQTTYILDMVQNAQLSDRHKKILEQQSMAIENL
                     II"
     gene            23213..23311
                     /locus_tag="LP327_001677"
     CDS             23213..23311
                     /locus_tag="LP327_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL1725792.1"
                     /translation="MKRGSSMLGITVMCLIHTSAAADRVMERKVIK"
BASE COUNT         7532 a         4094 c         4217 g         7484 t
ORIGIN      
        1 aatcagatac ataagcccat gtacccgttc gttgtaaata aagttcttct tgtaacaact
       61 cccattttct cttagaaatc ataatgacgc catcgttcaa tccattttta gaatccctag
      121 taatttctat ttcagaattt gaatgaacta cttcttgcaa cactttacct atattttctt
      181 tgaagtttgt tggagtaata gtatccaaaa tgaatacctc tcttctctat acctctatta
      241 tgaagtgccc ataaaataaa gcaagcgttg aggaataatt ctatcaacac ttgctttatt
      301 aagctttcat tttgtagttt gactaaaacc aataatctta tttctaaact ctcgaaaaac
      361 ttcttgtggt tgataatagt ctggatctat ttcaaatgtt tgtgagaata attcattgaa
      421 tttttctact gaaataatga ttttaccatt cttctctcga ctgttttcta ttatttctgc
      481 ttctaattca aataaatcta gtgacgaact aaaatcataa atcatgtcat attgaataaa
      541 aaaatcaaga aaaagttgct caaactcttc cttatttatc actaatgttt caaacggaac
      601 aactttattt tcctcagcca ttctcccagt tccattttca aaaatcacaa aactgatatt
      661 accttaaaaa ctactaacaa aataaaactt ataaagtaaa tggcaaccca actatgtaac
      721 cattctctct acgtactacg atttggtagt cctgcctttc aatcggtaat tcataatagt
      781 aatttactgt ttcttctttg ccactttcaa tatactctgg attatccaat atcatatcat
      841 actcataaag ttcttcacca tccttatttt caatcgacca ttgttgtgga cgtatcggat
      901 tttctgtatc atttataagt gtataggtca cttgtaatac acgatcatgt tgtgcaaatt
      961 catttctttt ttcggtcact tcaatatttt taaatgttag ttcaacccca tctgcctgat
     1021 aagtttcacc tatttttaaa tcttttgtta caatttttat tggcttttct tctggaaaat
     1081 gtctggcttt aattgttaga taacttccaa aaagaatcaa tcctagtagc actaaaagaa
     1141 ttcctttaac gagccactga aacgggaagt aaactttaaa catctttttt ctcctccgtt
     1201 ttgtatttgt tttttagaga atacgataac ttttgatcaa tgtcatccag cttttcaatt
     1261 acttcattac ttatcattga ttctagttct gaaatttttt gttcaaattt aatgctttca
     1321 ggatcattaa atttattatt tgatggacta gctaacactg gtaaatttga aaccataaca
     1381 cgaaacatat ttgcctttga accaaaaatt cctttgtttt ggttgtaaaa attgttgaat
     1441 atttctttag tttcttctga agccgagact ttaaaatatg gatagttcat ttttttcttc
     1501 cacctttctt tttttgggag ggagtctgta gggagcttgc tccctcagag atttacccgc
     1561 catctgactt gccagaaagt agcctagcgt gagcggggcg gattgactgg caagtagatg
     1621 ggtagctggc gtatgtccta aaaactgctt ctttccaagc aactcttcat aggcaaaaaa
     1681 ctggaaagtc cactaacaag cccattcaga attccatttc aaaattttgt tgcctttttt
     1741 gctgattttc aatcatttgt tgtgtgaatt cataatcctt tttagcacga tacattttta
     1801 catcgtcatt cattatgcga ctgatttgat agtgaatctg tttagcagaa ggtgtatgag
     1861 aacaattttc aaaacccttt gccggtagaa ataactcttt ctgatcagtc tttttaatag
     1921 aaattgtttt acttttcgca ctacgtaaag tttgaggatt cacatccgta tgaagtttaa
     1981 tcgtgtattc ttgatcatct tctaaagaca ttgtatttac aaaatgcttg tctacctctc
     2041 gcaagaagtc atcatattta atgaattttt catcgccttc acggtcgcta aattgctttt
     2101 gtgcgatgga atctaattcc acaaacggta catttaactc accagttttg taatttactc
     2161 cactaattag cgacataact tgctctcttt ccatattaaa aaaatttttt atatgtgatt
     2221 cttctaaatt cttttcaagt tgttcagaaa cagattcaaa tgaatatctt ttccctaact
     2281 ttcttgcact aaaagtacgt tcgacatact gacttttttc ttttctagat tgtttatctc
     2341 ttcctaaagt aattaactca cctctacgct catagaaatt tacaccttgt ttgtaaagaa
     2401 catagccaag tttgttattt gtttttgaaa attgataaac ttcaatgtta tattcttttg
     2461 ccaaatgatc cgaaaaatct tcaaaagaat tcacttcgca tttttccaac gttgcttcga
     2521 tcgcattttg cacaaactct tgataattaa tttttccata tttcatctta gaaggatcgt
     2581 ttgaataaag attggttctc ttttctaagt tttcatagta ctcattattc actgattcaa
     2641 gaccatgttc attgaggact tgatcgttaa tttttcgcat tagttctaaa tctttagggt
     2701 caatacgcaa ccttttacat gtttccatgt taatattatt aatgacaaga tgactatgaa
     2761 aatgatcttt atcattgtga cttgcaacaa tgatttgcga atttggaaaa gctttctctg
     2821 caaattcgac agctatttcg tgcattttat caatacttaa ctctttagaa gtactcttat
     2881 caaacgaatg aataacatga aacgcttgtg ctatatcttt agatttattc atcttggtac
     2941 gtactaaatt catttcttgc agtgcttcct cactatcatc actacaattt attcctgaga
     3001 ctgcaacaca tttttctttt tttgaaggat tagctgcata ttgtaatagc gaggataaat
     3061 ttttcttgcg tcctttcgat gttatagtag ctcccaaagt tgtttcacct catcttttaa
     3121 acttgcaata atctgaattg agtgaattaa attccgatca actttttggg aatactcttc
     3181 tttcgtaccc ggtctagttt gcttttctat ccaatattcg aaatattctt tttctacttc
     3241 tgataattta aaaaaagagg gggcactccg attggactcc aaaaaaatgt catctggata
     3301 actaatcatc aacttttttg ctatgctatt cgcaagttca tctttaaaaa tgttgtaacg
     3361 gctagcatca aaactaaaat gtaggctctt ttctttttta tcttcagatt cttctctaaa
     3421 atatttagca atgatattta atgcctttac cgcttgattc aaattgttac caatttgatt
     3481 taactgtttg gttaaaacta ccctatcatc ttcagctaac aacggcggtt catatttgag
     3541 tccctttaac cgctcacggc aaacgtctgc tctagaaatt cccctttgtt ttgcaatcaa
     3601 tgtcagttca agataagtct ttttatcgac acgaaaattt aaggcctcac tatgtttgcg
     3661 atttttttcg tacatttttt gtttatcatc cattgattat cccctcacat ttttttctag
     3721 agagcaacat ttgaaattgc tctctatttc atttttcatc tttaattctt cttgaatcat
     3781 cagttcaata taattagtga ccgtgatttt ttcatttgtt gcgttcttta ctgattcaca
     3841 taattcgtca agttctaaga aatcatgcgt actaatcttt agaattaatt gcatgacaat
     3901 cccccccttt tatttcttaa taaattatcg aaaagtggat taggatcatt aagtgtatct
     3961 ctgaataact cattgtcttc ataagtgaat cttcgtcttg attttgatcc cttatcattt
     4021 tcacggattt cactatcaat aatcactgtt tcatgatttg aatcaaattt tttcgctgac
     4081 tttctgtctt caacgatatt gattttttgt gttttttctt tcatttctcc actatctttt
     4141 tgaatgttct cccgaacatc tactgttttg aatccttgac tacttgtaga actttgtaca
     4201 ttttctcgga catctactgt ttttggttga cttgcactgc taccttgcac attctctcgg
     4261 acatccactg atttaaaccc ttgactactt gtagaatttt gtacattttc tcgaacatct
     4321 actgtttttg gttgacttgt actgctactt tgcagatttt ctcgaacatc aattgtctta
     4381 gtaccttgag catttgaaga actttgtaaa ttctctctta catcaacatt ccgtccaaca
     4441 tgaccattca gtgaaccttg aatattttct cggacatcta ctgatcggcc accatgacta
     4501 cttgtagaat tttgtggatt ttctttgact aatttttcta atcctgtatt tgaattttta
     4561 gcttcttgaa ttctttcttt ggtcaacgct tcttttccgt caatattagc gcctgtccgc
     4621 ccttctacac gtttccttaa cattatttcc tcgcgattca ttgcttcagg cgtgtatttc
     4681 gcttcatgac gcttctgatt aattaattct tcagcagact tagcttgacc gtgcatagca
     4741 tttttacgtt cttcaatatc ttgttggact ttttgctgaa ttgcgcttgc aggattatta
     4801 ttcgattcag cattattttt cattgcttca cgcatacgtt cagaactaga cataattcta
     4861 tcggcatttt caactttagc accatctttt ctatctgaaa gacgtgttgg cacggaatca
     4921 gcatattttc cagtttcatc ttctttattt ttagctaaca taggtttgct atttttatta
     4981 atagcggaca ctgtaccatc atctaaacct tctttaaact tattcttggt atcattagct
     5041 atatcacgca tactcttaaa tggttttgtt gctctttcac tagccattgt agctccatct
     5101 gaagctaaac cggataatcc acgttcatgg gcatatccca aagctcttcc agttttgcca
     5161 gctttttttg atagagtttc gccgaaattc ttttctggtt ttcgatcttc tccttctccg
     5221 cttcctttag cacgattaaa tccatttgat ccttttctaa acagcattgc gcccattcca
     5281 aaagcagaag caagttgccc atagccttct ttgagtccaa tatcaacacc aaagtatctc
     5341 atgactgttc cactgccttt gattaaaaca aaaacggcac agatataagc aattgttttt
     5401 ataaaaatat ttgttgagat accttgtcct gagttcagaa aattgataaa catagcaaac
     5461 attgataacc caaatccttg aaacccaaca gttaagtaac attgtagaat atctgataga
     5521 acaacttttg aacgctgtcc tgtctcaata tctgttgcaa aaacaagaac acctaacaca
     5581 cgtttaaatg caagttctag tattgctgta atgatcacaa acgcgctgaa cacataggcg
     5641 attgctaacg ctgtcaaccc aattgaaatc ccccatagat ttgcttggta tcggtaatag
     5701 cctgatttca agctatcaga aaatgttgat aagaagttgt cagaaaactt tgtagcaaca
     5761 aattcattat tagaattttc aactaattca taacgaagat tgtccgcaat tggattttct
     5821 ttttccaatt tatcaatcac tttatccgtt aacacttgtg tgagatccgt ctgttgaaaa
     5881 ttttttcgtg ttaaattatt tcgatcctcc gttttatcaa tgcttgagta ctgatctgtt
     5941 ttattaatat atgctaaatc tgttactcct tgttttatca atgaccaagc gactccgtca
     6001 tctgaatttg taatcgtagt agagtcctta taaaatgttt tagcaaattg aataccacta
     6061 gaaatcatgg ttggcattaa aagtattaat gctatagaca tcactacgtt cattccaacc
     6121 gattttaaat ccactgttcc ttttccagta atcattttgt aaccaacaaa catcaatgaa
     6181 aggatcatta atccaatgac aattgtattt acggctgatt gataaacatt atttagccct
     6241 gtactttcaa tgaaattgaa caaatccagc acatctggaa tcatattttg aatcgaatct
     6301 gtcacagtaa acatgccttt taccacagcc catccgagcc aaatcccgaa ctgactaagg
     6361 aaatctccat tgtacttttt taggtaaggt ctataaagag aattattcaa aaactctgtt
     6421 tgactttttg tgtcatcaaa ccaattgaaa atactcgcat gagcaatatt acttgtaaaa
     6481 ataatcatag ctatcagaac aagaaaatta acaaccaacc aatttatttt tttattggca
     6541 atcttttttt caattatttt catcgttacc tcccttagcc cattttctga ggctctcaga
     6601 gagcttctga gccatttttt gattaattat gacatgagtt acacaattat cattttcagc
     6661 tcttaaatcg agaactatag tgttgaagca aatagtctcg attaattcga gactatctgt
     6721 attttgatct agacaccaaa cttgatctat taaacgattt ttaatcaaaa taacccctcc
     6781 tatattcaaa gttctcaaat gaagtggaga tacccctcca tttcatttga ctcaattcta
     6841 ttcttattgc ttatctattc cttcgctgat ttgcaattca attagttcta acgctttatt
     6901 ttttagagaa gcaaagatat aatcgtcttt ttgtttgata cgatgatctg tttttgctct
     6961 tcgtatcaag tcacgtaaag tcattgatag ctgttctcta acatgatcta caaaatactt
     7021 gttattacaa atttcataga taattttcag ttgaattttt tgttcttttt cagcagatct
     7081 aatcgcatat ttgaattttt tgtaccagtt gtcgaattga cgataatcac tattgctagc
     7141 cagttttaag caattcacaa tactttctcc ccatatttgt ttaagaagat tttcttcaat
     7201 aaatgtttcg atcaattctt catctaattt attattttta gttgaaagtg attcaagaag
     7261 atcatcatta tgtgattctt taatatcttt actatcttta taagacttat ataattctag
     7321 ttcatttttt gaactatcag caaaattgtt tttgtctctt gttttcattg atgtgtcgca
     7381 tagttcaaat tttgaattat caacagaata ttgatttgtt ccatagttca aattttgaac
     7441 tatcaagttt ttttgtctat tttcttgatg ggttatctta ttaattttat aaacatcatc
     7501 tttgcacaga acaggcttta acaaataata aaaatttggg aacatttgca ttttactttt
     7561 attgaagcca ttttttcttt tcacaagaag tttaactttt tgaagttcat ctagtgctga
     7621 taagattgta ttttttgaca ttcccgaaat ttctacgaga tttttgattt caaatatcaa
     7681 aaaaattttt tgattctcat catacaagtt atttttcaat gaaagtctat ttctatcttt
     7741 tagtaacata taaactaact tagaacttag tttcattttc ttatattttt catttacgtt
     7801 taaaaaaata ggatattgat agtataattc tgcatatata tcagaattag aatagccttg
     7861 cttttcaact aagctttcaa cttctttttc attcattgtc acacctgctt ctgattttat
     7921 ttgctataat agattcaaat aaagttttct cagaacttta ttattgagtg ttcccccgat
     7981 ctgcaaactg acgggaacac tctttttaat tttttctact tcatcaaatt taaaaaacga
     8041 ggtgtttttt aaatttgatg actcaatatc cattttttgc attcttcttt tgaaaacaac
     8101 tccatttttt ctaataaaat aactggcaat cccattcttt taaatttcaa aaaagttgta
     8161 tatgatacat tcaaatattt agccatctct tttcctttta gaaatggtct ttctaatccg
     8221 gtatcaattc ttgcttgatc aatagcctct tttgtcattt caaaaacata atttttcaat
     8281 tgattcgttt gttgtttatc taacattact gtaattcctg catccattat ttttctcctc
     8341 tcattttcta tttcttcaat cagccaactc gccaagttgt atttatttag attttcaaat
     8401 cattaagtaa ccaatttacc aacttttgat aagtagatgc tctaatactt tctttgctcc
     8461 cattttcgat tttgacatat gttttccgat ttatacctat caattttgct gcatcgtttt
     8521 tagtaagtat aagatcggct ctatgtcttc ttaagcttct aatgaattga tcattaagag
     8581 ttatcattca gcgcctcctt tcaaattatt cactttgtgg tttttatctt aataagcaca
     8641 ttttaaacca caaaacgagt aatatcaatt aaaatattac tcaatttgtg gttttcatgc
     8701 tataatagtt tggaatttac tatagggggc gttactttga atagaataaa attttatatt
     8761 gattccagta aaaagaccat taaacaaatt agtacagaaa ctggtattaa ctatacaact
     8821 cttacgaact attaccgtgg tgagcgaaat cctagaaata ctgaaacatg gaatatcctt
     8881 gctgattatt tccatgttcc tgtaagttat ttgatgggat tagacgaaac aaattccata
     8941 gaacaaccta atcttgatag aataaaaact gctcgtttag aaaaaaagga caaaatagtc
     9001 aagaatattg ccagtcttga ctcagattat tttactgaat tatctaatct atacttttcc
     9061 atgacttctt tacttgaagt caaacttcaa gaagatgact ccgactttct ttttttaaaa
     9121 agtattctag aggactcctt tgaatacacg attctatttg ccaaacaaaa gaagtttatt
     9181 aataaagctt taaacttaaa tatcgatgaa agaaaagagt tagaatctga gttatttggc
     9241 aacttaaccc aagtacaggt taacaatcta aaaagagaga taacagctcg attaatatca
     9301 aaacttcaat aaaactaatt attcaatcag ccaactcgcc aagttatcag attggagaga
     9361 ttagttatgt caatacaaaa gtacacaaca aaaagcggtg cacgatatag tgtccgcttg
     9421 tatgttcgta aagatgaaaa tggaaagcaa gaatactatg taaaaaaagg gtttaaaact
     9481 gaaaaagaag ccaagttaca cgaagcaaga aaaaaggtcg agattgaaga taccggctat
     9541 cataagcctt ctaatgactt gtttataaca gtgtatgaag aatggttgcc ttcttatagg
     9601 aatcgtgtac aagaaacgac atatcaacgt acaaaagacc ttttccgctt gcatatacta
     9661 ccaaaattcg ggaataagtc catttctaag attactcctg ttcactgtca gaatgcagtc
     9721 aatgaatggg ctttaaaatt caagaatatc aaacagctta agtcttacag ctctcaaata
     9781 tttgagtatg ccatttttgc agagctatta caccgaaatc ctatggcaaa tacaaatcta
     9841 ccaaagcggg aaaagaaaga aaatgaaaat tacttttcat tagaagaact aaaagctttt
     9901 tgggatattc tcaataaaga agagccttta aaacatattt taatcttcca gcttttgatt
     9961 actactggta tacgtaaagg agagctagct gctcttcatt ggtctgacat ttattttgaa
    10021 gaacagctaa tgtatgttag aaagagctat gcaactattc gaaatgaaga gaagaatgca
    10081 agaagaaaaa ctgtcaggat tcaaaaaaat acaaaaaata tatcttccga acgaatatta
    10141 ccaattgatg cacaaactat tgaaatgtta aaaatctgga aaaaagaaca agctcgagag
    10201 cttttgcagt ttggcataaa tacaaataca aaaaatcaac ttatctttac ctatgtaaat
    10261 gcagacggtg acattaatca acctttgcat gcggactatt caaataatat attgaaacgt
    10321 ttagagaaga aatataaatt taagcacgtt actattcatg gattccggca cactcacgct
    10381 actcttctac tagaaggtgg agcaagtatc aaagaaacac aagatcgatt gggtcataaa
    10441 aatgctgaaa ctacccttaa cacttattct catgttactg aaaaagctca acgaaatgcg
    10501 ttagaaaaat tcattgctta cactggttta taatattgtc attacctttc tcattaccag
    10561 aacgaaaaaa agacaccttc tcgcaatgag aaagtgtctg aaatcgttgg tataacaacg
    10621 attaacgttt tgaaaactgt gaagctttac gtgctttctt aagacctggt tttttcttcg
    10681 tatcattgaa taagtttgaa aaaccttgat ttataaaggt tttcccgtaa atatatttta
    10741 ttatatttga ttaaatttga aatgtttgtg ttctaaaatg tgttctaatt taaaggcagt
    10801 atatttcgta ttttagatta aatttttagg ctcattcatt ctcaaatcca ttctattcag
    10861 tcaataaaaa ttttaaaaag actttataaa caaagatgtt caagcgagca tcttattttt
    10921 ttgtgcttaa tttgaaagga gaacatttca tgtataaaaa acaaaaaaag aaattatgtg
    10981 tattaactat tttgagtttg gctgttcaag caattgttgg agcaattgct cctacgatta
    11041 cttttgctga tgaaattacc catccacaaa cggtaaccgt agaactagat ttagcacatc
    11101 aatatgctgt tgaaggaact tttagtgatg gtcgtccaat gtcagaagtc actgttccgc
    11161 attatgcagt gtacaacggc tttaaacaag atattttctg tattgaacca ggagtcccga
    11221 tttacaatga gttcactcct ggctatgaga aaaacccgtt accagatatg tccgataaag
    11281 ccaaattagt ttccgtttta tggaaaaaag taggtacaga tgcagatact catattgtgg
    11341 cacagaagat gatctggcaa gaagttaatg ggtataccct tcactcaata aaacgatcag
    11401 atggtagtgc cgtaaatatc gcagccattg aggcaaaaat aaatcaagcc attgacgatt
    11461 atcagaagaa accaaccttc cataatagta cggctaaaac agtattaggg caatcgacta
    11521 ctgtgacgga tacgaataat ctgaatttgt cagagttcga tgaggttgtg gaaaatgccg
    11581 caaacattga ttatcgtgtg aatggtaatc agttgataat caccccaaat gccaattcaa
    11641 aagaaagtgg tgtgctgacg cttaagaaat ctgctggtac cggaacacct gtcgcctata
    11701 aaaaggctgg ccaacagact ttaatggccg gggcgattga taaacctaat acctacacaa
    11761 tcaaaattga tgtggaaacg gagggttctt taaagatcaa aaaagtcgat aaagaatcgg
    11821 gtgatgttgt accaggaacg gttttccatt tagactttgg aaaaacttta cctgcaaaag
    11881 acgtgactac tgacaaagaa ggcattgcta cattggatga gattcctcat ggtacaaaag
    11941 taaccattac cgaaaaatca gtaccggcgc cttatacgat tgataccacc cccatgacta
    12001 ccaccattaa agcaggcgaa accatttacg tgacttcaaa aaacgcacgg gaaaaagggc
    12061 aaatcattct ggataaaagc ggagttgaaa cagggagtga tctttggaat gacaattatt
    12121 ccttagctgg taatacgttt gccattcgca aagacagtcc aaccggtgaa atcgttcaag
    12181 aaatgaccac ggatgaaaac ggtcatgcgg aaacaccaaa agagattgct aatgcgttgg
    12241 aattgggaac ctattatgtg accgaaacta aagctagtca tgggttcgtg aatacgttca
    12301 aacctgtaaa agtcgaattg aagtatgcca atcaaaccgt agcccttgtt accagtaacg
    12361 taaaagggca aaatcaagaa gtcactggcg aaaccacttt gacgaaagaa gacaaagata
    12421 ctggggataa agcacaaggt aaggcagtct ttgagggtgc tgaatacacc ttgtttactg
    12481 ctaaggatgg aaaagctgtt aaatggagtg aggcttttaa accagagtta gcgaagggaa
    12541 cgaaaggttc tgatgaaacg gtcactttga ctttagacga aaagaaccaa gttgccgtta
    12601 aacatttagc tatcaacgag tattattggc aagaaacaaa agcgcccgaa ggatatagcc
    12661 ttgatgaaac aaagtatcct gtatccatca aaaaggtgga tgatagcgaa aaaaatgccg
    12721 tgattacccg aaatgttacg gcaaaagaac aagtgattcg ctttggcttt gatttcttta
    12781 aatttgctgg atcagctgcg ggcactaccg aaactggctt caatgatctg acctttaaag
    12841 tgtcgccatt ggaagggacc aatgaaatca caggtgccga agatgaagcg accacagctt
    12901 ataacgagca attagggttt gatggttatg gcaagtttga aaatcttcct tatggggatt
    12961 atttacttga agaagtagaa gccccagaag gttttcaaaa gattacgcca ttagaaatcc
    13021 gttctacttt taaggaaaac aaagaagact ttgtgaagag tgagtatgtc tttaccatta
    13081 ccgaacaaga ccaaaaacaa ccaattaaaa cggtgaccgt tccttacgag aagctgacga
    13141 acaaagcatt ttctgttagt ttgaaccgtt taatgctcta tgatttgcct gaggaagaag
    13201 atagtttaac ttctcttgcg acttggaaag acggaaacaa agaattaact tctctggatt
    13261 ctaccgagct agttgataaa ttgagttaca atctacatga aatcaaagaa gactggtatg
    13321 tggtagcgca agccattgat gtggatgcga caaaagctgc ccaagaaaaa gatgaaaaag
    13381 ccaaaccagt ggtgattgca gaaacaagcg caacgttggc gaacaaagag aaaacaggaa
    13441 cttggaaaat tcagcataaa ttaaccgccg aacaagtttt aaataaaact attgtcttat
    13501 tcaactatgt gtatgaaaat aaggaagctt ttgaagcagg cgataagcca gtagcgaagg
    13561 atgttagctt gaataatcaa gcccaaactg ttaattgtac ggtggaacgt catgtttcta
    13621 tccaaacaaa ggcccaccta gaaaacggtt ctcagacctt tactcatggt gatgtggtgg
    13681 atatgtttga tgatgtgtcg attactcatg atgtactgga tggctcaaaa gaagctttcg
    13741 aaaaaattct gtatgcttta ttaccggatg gtacaaacaa agaaatttgg aaatctggca
    13801 agattgatta tgaagtaaat gataaagaat tcaccaaaac tgtgcttgcg aaaaaagtag
    13861 ataccggaaa atatccagaa ggaaccaagt ttacttttgc ggaaatcaat tacgataaag
    13921 atgggaatat caatggaaaa cacaatgaag atttgaaaga aaaatctcaa accttaacac
    13981 caaaagaagt tccaaccata ctaagtacgc cgaaacaacc ggaaacacca actgttccaa
    14041 gtggctctca agaatctagt cccacagtga agaccttccc tcaaaccggg gagaaaaatt
    14101 ccaatgtttt actgttcatt ggttttactt tgatttttgc gacggcgggc tattatttct
    14161 ggaatcgccg gaactaaggt gatgagatga tacaagtaaa tcctaagaac caacgtgcgc
    14221 gaccgcctcc taaaaaagta gtcgcgctat ttttgtatcc aaaactaaca atgaaaagag
    14281 gaaaagaaga tggaattgaa atttgtcgtg ccagatatgg ctgaaacctt tggaaagtta
    14341 agttatgccg gtgaaggaga aattataaca gaaggatatg gacggaatac tacggtgatt
    14401 ggtcgcagtt atcatttgta ttccagtaag caacgggccg atgatattga ggtggtagta
    14461 gctgcggaag ccggtgaaaa ggattttgat caagaccaac cgctaaaagc cgtgaatccc
    14521 catttggttg ccaaaggcta tgagattgag aatcgtgggt ttactgatta cgtgttgtat
    14581 gttgatgatt tagtgaaagc ataggaggaa aagaagatga gattaacaga aggtattgtc
    14641 gtagattcag gtttaacatt tgggaagttg cgtttttccg cattacgccg agaagtacgg
    14701 aagcaaaatg aggatggaac aattggtaat gaggtaaaag aacgaaccta taatttaaaa
    14761 tcctctgcac aagggcgaat gattcaagtt agtatcccag ctaatgtacc cttacgcgag
    14821 tttgcttatg atgcagaggt agagttggtc aatccaatcg tggataccgt tgccaattat
    14881 gtttttcgag aaggaactac cgttaattgg ttcattaagg cggatgattt agtcttgaag
    14941 cggcagccga atcaaggaaa tcctacgaac caaaatgaaa gaaagaaata ggtgaagatt
    15001 atggaaatta atgttgaaca tattttagat tgtctcgatc aatatggaaa aggcgagctg
    15061 actgaggaac aactcgttaa agcattaacc tctgatgaaa aaatgtttct gattatacat
    15121 caaggtttac ttggagaaaa tagcgacagt gaagaagact ctgatgtact agactggtta
    15181 ggagaagaag agtctttctt tatagttgtc gaagtaaatg aaaacttatg tcggcaggca
    15241 gaatccattt tggaagaaat tggtgtggag atgccggatg ctattgaagg ctttttaaat
    15301 caattggttg aaacgaaaca actcccctcc tcgtagcagt aaatgactag cataactttt
    15361 tgaaattgcc taatgaaatc ctattgggct ttttcgattg gaggtggaaa ccatcaaaat
    15421 gtacaaagga catcgcatac gcgcaggaga tcagcacttg gtttatcact ttgttcttgg
    15481 ctggctactc gctctgttca tcggttggat gagcgtcttt tattttcaag agtttagaca
    15541 atttgatatt tctaagctat cactttctac tattgaaatc gtttggtcta taaaagattt
    15601 agtatgtctg cttggaagtc tcgctttttc aggagctatg atattgcttt atattcactt
    15661 ttttcctgat cattggcgaa gcttgtggca tcgacaaaag ctagctcgga tgattctaga
    15721 gaaccattgg tatgaagtga aacaaactca aagtgaaggc tttttcaaag atttgaatag
    15781 tagccgaacc agagagacta tcagttactt ccccaaaatc tattatcgaa tgaaagatgg
    15841 attactgtct attcgcgttc aaatttcact tgggaaatac caagatcagc ttttaaaatt
    15901 ggaaaagaag ttagaaagcg gactgtattg tgagttagta gaaaaggaac tcaaagactc
    15961 ctatgtggag tatactttac tgtatgatat gatcgccaat cgaattggga tagacgaagt
    16021 agtggcagaa aatgggactc tgcgattgat gaaaaatcaa gtatgggctt atgattcttt
    16081 accccatatg ttaatcgctg gtggcacagg tggagggaag acctatttcc tcctcaccat
    16141 tatcgaagca ctattgaagt cagatgcaga attgtttatc ctcgatccca aaaatgcaga
    16201 tttagctgat ttaggtacgg tgatgcctca tgtttattca caaaaggaag aaatttctgc
    16261 ttgtgtggaa gatttctatg aacgtatgat ggctcgtagc aaggcaatga aagaaatgtc
    16321 taactacaaa acaggagaga attatgccta tcttggactt ccacccaact ttttaatctt
    16381 tgatgaatac gtggcttata tgggtgcgaa tcgttttcct acaagcatcg aatagatgtg
    16441 aaatggaagc ctattgagat tatcaatagt gacaactaat gttttgagag atggaatgaa
    16501 agccttcaag ttgagctgaa acatccccgc taatactcct acatgctcta atcaatgaag
    16561 tagattggtt ttggtatgaa gctaggtgaa gtcgtctgaa atctgcctaa gtgcttgagc
    16621 ataaggctga ctatagggcg ggcggctgaa aaagataact atggtaagaa tgtctgaaaa
    16681 tgactgacga atctgcgaat gtacgggtct aaaaggctga tcttattgaa agataggaac
    16741 atacggacgt atggtgagat accgacaagt aaggttccat gatatgaaaa tcgggatagc
    16801 taacgaagaa gctataattt cacaggctca tagtagatac ctaaggttat acatgatagc
    16861 taaaaacatt ggaacgatgt aagcagaaaa ctatcaatta aatgactact tctagatagg
    16921 tgcatataag taataacgaa attgctttgt ttctgtgaaa gtaagggcac agtaccgatg
    16981 aagcgtctaa caaatgtgga gggatagccc taagtctatt taataacaat gaatgttgaa
    17041 acagatgaag ttcaaggatc tggtaagaac gtgggaaaat ccgttatgga ggtgccacgc
    17101 ttgaacacaa aaatacgtta ctgggaatat tatgatctac aagaaacatt cgatcagcta
    17161 tttactcaaa gtagaaacgg gaaaaagttt tatcaattat atgagttaat catttctgaa
    17221 aataatattc ttttagcata ccgtactatc aaggcaaaca aggggtcttc gacaccagga
    17281 acggattcct tcacaattga taactataag gaaatgaacc aagcagagtt tatccatctt
    17341 attctcagtc atttagaaaa ttacaaacca aaatccataa agcgagtaat gatacctaaa
    17401 cccaatggtg agaaacgccc attaggaatt ccatgcatga ttgatcgaat gatccaacag
    17461 atgttcaagc agatactgga gccaatatgt gaagctaagt tttatgaaca tagctacggt
    17521 ttcagaccat tgagaagtgc aaagcatgct ctaggtcgca ttatgtatct cataaacatc
    17581 agtaaaatgc actatgcagt agacattgat atcaaaggtt tctttgataa tgtaaaccac
    17641 cgtttactga taaaacaatt atggaatata ggaatttgtg acaaacaagt cttagctatt
    17701 ctttcaaaat ctctgaaatc acccattcaa ggagaaggta tcccgagcaa aggaacgatc
    17761 caaggaggta tcatttctcc actattatct aatgttgttc taaacgactt agaccattgg
    17821 gtctcaaagc agtggcatac ctttgaaaca aagtatccct atactaaagg ttacaataaa
    17881 tttcgtgctc taagagatac aaatctaaag caaggctata ttgtcaggta cgcagatgac
    17941 tttaaaatca tgactaatga ttatccgaca gcattaaaat ggttccacgc tgtcaagctt
    18001 tacctaaaag acagacttaa attagatatt tccaatgaga agtctaaaat cgtcaatttg
    18061 cgtaaatgta agtcggaatt tttaggattc gctatttgcg tgaaacaaaa agggaaaaaa
    18121 tgggtttgta attcacgtat ttctaacaaa aaaaaggatc agattaaaga agagattaaa
    18181 caaagaatca aagatattca aaagagtcct acagcacaaa atgctctcct gtttaatgct
    18241 ctcgtgctcg gtgttcataa ttatttcagg tatgccactc atattaattt agatttgaat
    18301 aggatgaact acgttctttc attcactact tacaaccgat tatcaaattg tagtacaaga
    18361 tgttacccca acaaagcttc cccaacttac aaaaagtttt actcaacaac gatgaaaacg
    18421 tataaagtag ctggagtcta tttgtaccct ctttgcgacg tatctacaaa aacaatttac
    18481 ggatttaata cggaggatac accgtttact ccgtttggtc gtcaaagatt ggagcacaaa
    18541 agcctacagt ctcttgtgtt ccaagaatta agaaaattga tggaatcgaa aataccgaat
    18601 agaacggttg agtatttaga tagtagaata tctcgttact caatgaaatc aggcaagtgt
    18661 gagattacaa aacaattttt gccagcaaaa gccgttcact gtcatcacta tttgccaaaa
    18721 agtcttggtg gagacgacaa attcgataac ttgagaatta ttcataagga tatccatctt
    18781 ctgatccata caacaaataa aatgataatc gatcactacg tgcatgaact gaaactggtg
    18841 ccagagcaaa tagcaaaaat caacctttat cgaaagatgt gtaatctgca aaacattcaa
    18901 tagaaaataa atagatggaa cgccgtatga ggtgaaagtc tcacgtacgg tgtggagcgg
    18961 gggaaaagat ggagattata tcaaattctt acctatcgct atagatgtta acgacgaaag
    19021 aaagtgcggt gattttgaat aagctcaaac aaatcgtgat gttgggtcgt caatctggtt
    19081 tctttctgat tttggcttgc caaaggccgg atgcgaaata tttaggagac ggaatccgcg
    19141 atcaattcaa ctttcgcgtg gctttgggtc gtatgagtga actcggatat tccatgatgt
    19201 ttggggaggt cgaaaaaaac ttctttatga agcacatcaa aggtcgaggt tatgtggata
    19261 caggtggcag tgtgattagt gagttttata cgccacttgt accaaaaggg tatgatttct
    19321 tagattcaat tagtaaatta gtaaattaga aaagatgaat gattgagtga tcttcaatac
    19381 tgactttata cttcaagtaa cgtataatac ttttatatag taggaagaaa ggggataatt
    19441 aatatggaaa gtaatgattc ttatttagag tatgcaaaag caaataagga taagtttatt
    19501 tcttatatta tcaaagggaa atcccccgaa gttggacgag acgcagtttt tatggcaggg
    19561 agtcctggag ctggaaaatc agaggttgcc attggattag ctgagaatta tgataaccat
    19621 gtgattattg atgcggatta ctttcgcacc cagtttcctg attataatgg aaaaaaactc
    19681 tagtctcttt caaaaagcat cttcttggtt ggtagagcag gcatttagat atgtcttaga
    19741 tcatggctat tcttttattc tggatgcaac ttttgcaata ttaagtgctg aaaaaaatat
    19801 ctcgcgggcg ttgaaaaaac attttcgagt gacgattttc tacgtttatc aagaccctaa
    19861 aatcgcttgg aaatttacac aagaacgtga aattgcagag ggacgtcatg tgccgaaaaa
    19921 ggtatttatt aacgcttttt tcaaatcaag agagaacatt gcaaaagtaa agagtaaaca
    19981 tccagatgta ttgcttcata tcataataaa agattatacg aataatattt ccgaagttca
    20041 ttatgctgcg gacaatctag aattattttt accactaggg tatagtagga aagatttgga
    20101 ggttcaatta catgactgat aaagaacaag taacgaaaat tgtaaagaaa tacaataaga
    20161 gcattgcgga tttatcggaa aatgctactg caaaagagtt caaaacggtg attaaatatg
    20221 tagccgatca ggccaatgaa aaacaacgga aattagttgg attaaataaa aaatagtagg
    20281 acaaattaat aatgtatgag ccaagagcaa aatgttcttg gcttttttct ttgcaatgaa
    20341 tgaggtaatg tatgagattt ttagatttat tcgccggtat tggtggcttt cgtttaggta
    20401 tggaacaggc gagccatcat tgtatcggtt tttgtgaaat tgataaattt gctagacgta
    20461 gttataaagc aattcatgat acaagtaagg aggtggaaat gcatgacatc acaagtgtat
    20521 cagatgagtt tattcaatcc ctcggaccag ttgacatcct ttgcggcgga tttccgtgtc
    20581 aagctttttc aattgcggga aagcgacaag gattttccga tactcgaggt actttattct
    20641 ttgaaatcgc tcggttcgcc gctcttctcc gacctaagtt tttattcctt gagaacgtcc
    20701 ggggattgct caatcacgaa gggggggcta cgttcgagac gatcctccga acgctggatg
    20761 gattggggta tgacgtggag tggcaggtgc ttaactcaaa ggcctacatt ccccaaagcc
    20821 gggagcgtat cttccttatc ggacattctc gagacgcatg taccgaacaa gtatttccta
    20881 tcattggacc gtcttcaaca tctgatcaaa acatcagaaa tttggtaaat atcaatcctt
    20941 ccaatcgagg aatgggtgga caagtctatg gttcagatgg cgtagcgcca accttaacag
    21001 gtgatgaagg aatcaaaatt gcactgccag caagggatgg aatctctgta gcaggactat
    21061 taccgggaaa gtttgaacaa ggtaatcgag tttatgaagt tactggaacc gcccccacct
    21121 tgtccacgaa acaaggtggt acaaaaatca tgattcgaaa aaatgaaaca gaatatcaag
    21181 aggtgaagcc aggagacagt gtgaacctgg cttttcctaa ttccacaagt cgccgaggtc
    21241 gtctgggaaa acagagtgtt catactttac tgacaggaga ccaacaagcg attgtcacgg
    21301 atcagtatca aattcggaag ctaacaccta gagagtgttg gcgacttcag ggctttcccg
    21361 attgggcttt tgatcgagct tctcaagtca attcagatag tcagttgtat aagcaagccg
    21421 gaaactctgt aacagttccg gtcatttttg atattgccag aagattaaag gaggtacaaa
    21481 agtgtgattt atgatgagtt ggttggagaa atttattggg tgattgaaaa gattcagtca
    21541 gacccagaat tagaagaaca attgcgacgg ttaaactttg atattcgcaa gaatggtgtc
    21601 aaagtcgctg gcgatccata tttaatgaat gaggagacgg atgctcgaat tgaaataaac
    21661 caagtaattg cagaatttga gcggattgcg gaccaagcaa aagaaccgga tattcgtcaa
    21721 tacttgttag aaatgaaagc agagttggaa gtaaatggta ttacagatga gtaacctaaa
    21781 ccaacacaac tgactgttag gcgctgaccg cggaggattc gcgaagcaga aatcctccgt
    21841 ggtgctgtgc ctgttcatgg acggcgttag ccggccatga tgtctcaccc cccgtatcta
    21901 acaggggggt acaaaaacac aaaaaacaag caagcagcga ttaagaatgg ttgtcatgac
    21961 gaggatagtt agctgtttga acaggtgtta actcattttg aaaagttaac accttatttg
    22021 gaggtgaaaa cttggtacaa cagaacttag attatcgttt actaaaagat cggcgcaatc
    22081 aatatggtgt ttctcaaaat aaattggcta ctgcttgtgg attgagtcgt ccctatttga
    22141 atcaaatcga aaatggtggc gtaactacat ctacaaaaac tatgagaaaa atttttgacc
    22201 aactggaaag tttcaatccc gatttgcctt tgacgttact gtttgattat gtaaggattc
    22261 gttttccgac aacggatgtt caaaaaattg ttcaagagat tttgcatttg aaatttgact
    22321 atatgcttca tgaagactat gccttttatt cgtaccaaga acaatacatc atgggtgata
    22381 ttgtggtgat gctttctcat gaggaagaga aaggcgttct tttagaatta aaaggtcgtg
    22441 gttgtcggca gtttgaaact tttttgctcg ctcaaaagcg tagctggtac gactttttcg
    22501 atgattgctt aaaagctgga ggcgtcatga aacgcttgga tttggcaatc aatgatcgag
    22561 ttggcttatt ggatattcct gagttgacga aaaaatgtca gaaggaagaa tgtatctcct
    22621 tattccggac ttttaagagt taccgctcgg gtgaactttt gaaagctgat gaaaaggatg
    22681 gaatgggaaa tactttgtat atcggaagcc ttaagagtga ggtttatttt tgtttatacg
    22741 aaaaagacta tgaacaatat atcaaattgg gaattccgtt agacaagagc gaaacgaaga
    22801 atcgctttga aattcgattg aaaaatgacc gtgcctacca tgcaatacaa gacttgttaa
    22861 aaggtcgaag tattgagggt accacatttt ccattatcaa ccgttactta cggttcacgg
    22921 ataaagttga agggaaacgg cgaacaaact ggccgctaaa tgaacgatgg ggtcgcttta
    22981 tcgggcgaaa tcgcaaggaa attcaactga cttctgaccc caaaccttat actatcgagc
    23041 gaacactgag ctggctaggt cgacaggtag cacctacgtg gaagatggct aaggagttag
    23101 atcggttaaa tcagacgact tatattcttg atatggtaca gaatgctcag ctatcggatc
    23161 ggcataaaaa gattttggaa caacaaagta tggcaataga aaacttgatt atatgaaaag
    23221 aggaagcagt atgcttggta ttaccgtgat gtgcttaatt catacaagtg cggctgcaga
    23281 tcgagtaatg gaaaggaaag tgataaaatg aatttcggac aaaattt
//
LOCUS       DAFEHS010000042        23276 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFEHS010000042
VERSION     DAFEHS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.043"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..129)
                     /locus_tag="LP327_001678"
     CDS             complement(<1..129)
                     /locus_tag="LP327_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL1725793.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDS"
     gene            402..2873
                     /locus_tag="LP327_001679"
     CDS             402..2873
                     /locus_tag="LP327_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL1725794.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2976..4712)
                     /locus_tag="LP327_001680"
     CDS             complement(2976..4712)
                     /locus_tag="LP327_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBL1725795.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4709..5413)
                     /locus_tag="LP327_001681"
     CDS             complement(4709..5413)
                     /locus_tag="LP327_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1725796.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5544..6047
                     /locus_tag="LP327_001682"
     CDS             5544..6047
                     /locus_tag="LP327_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBL1725797.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6007..6345
                     /locus_tag="LP327_001683"
     CDS             6007..6345
                     /locus_tag="LP327_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBL1725798.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6366..7784
                     /gene="ffh"
                     /locus_tag="LP327_001684"
     CDS             6366..7784
                     /gene="ffh"
                     /locus_tag="LP327_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBL1725799.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7896..8474
                     /locus_tag="LP327_001685"
     CDS             7896..8474
                     /locus_tag="LP327_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBL1725800.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8478..8999
                     /locus_tag="LP327_001686"
     CDS             8478..8999
                     /locus_tag="LP327_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL1725801.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9078..9632)
                     /locus_tag="LP327_001687"
     CDS             complement(9078..9632)
                     /locus_tag="LP327_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL1725802.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9885..10160
                     /gene="rpsP"
                     /locus_tag="LP327_001688"
     CDS             9885..10160
                     /gene="rpsP"
                     /locus_tag="LP327_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBL1725803.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10172..10423
                     /locus_tag="LP327_001689"
     CDS             10172..10423
                     /locus_tag="LP327_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBL1725804.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10547..11338
                     /locus_tag="LP327_001690"
     CDS             10547..11338
                     /locus_tag="LP327_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725805.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11508..12029
                     /gene="rimM"
                     /locus_tag="LP327_001691"
     CDS             11508..12029
                     /gene="rimM"
                     /locus_tag="LP327_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBL1725806.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12019..12780
                     /gene="trmD"
                     /locus_tag="LP327_001692"
     CDS             12019..12780
                     /gene="trmD"
                     /locus_tag="LP327_001692"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBL1725807.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12916..13263
                     /gene="rplS"
                     /locus_tag="LP327_001693"
     CDS             12916..13263
                     /gene="rplS"
                     /locus_tag="LP327_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBL1725808.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13637..14299
                     /locus_tag="LP327_001694"
     CDS             13637..14299
                     /locus_tag="LP327_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725809.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14378..17605
                     /gene="fss3"
                     /locus_tag="LP327_001695"
     CDS             14378..17605
                     /gene="fss3"
                     /locus_tag="LP327_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL1725810.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17719..18033
                     /locus_tag="LP327_001696"
     CDS             17719..18033
                     /locus_tag="LP327_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL1725811.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18046..18420
                     /locus_tag="LP327_001697"
     CDS             18046..18420
                     /locus_tag="LP327_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL1725812.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18430..18774
                     /locus_tag="LP327_001698"
     CDS             18430..18774
                     /locus_tag="LP327_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBL1725813.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18845..20197
                     /locus_tag="LP327_001699"
     CDS             18845..20197
                     /locus_tag="LP327_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL1725814.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20257..21399
                     /locus_tag="LP327_001700"
     CDS             20257..21399
                     /locus_tag="LP327_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBL1725815.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQTFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21424..21678
                     /locus_tag="LP327_001701"
     CDS             21424..21678
                     /locus_tag="LP327_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725816.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21934..23118
                     /locus_tag="LP327_001702"
     CDS             21934..23118
                     /locus_tag="LP327_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL1725817.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23115..23252
                     /locus_tag="LP327_001703"
     CDS             23115..23252
                     /locus_tag="LP327_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL1725818.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7732 a         4138 c         4994 g         6412 t
ORIGIN      
        1 agagtcatcc aattttcctt taataaactg cgcttgaacg cctttatatt ctttttcaat
       61 cagccattct tcatcaaaaa ataaatgttt atctgttaat ccgagtaatt ttctagtatg
      121 attatccatt gagagactcc tttggtagga tgacgttttg gtcgactaaa ttttaccaaa
      181 agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt
      241 ttccaccaac acaaaatatt atagaaccct ttttttttgc tatatgacag gaatgaaagg
      301 tgtttgcaag cttaatgtga ggaacacttt ccatatctgt tctatactgg ataggagaag
      361 tttggaaata tccagaaaac gaaatcaatc gaggtagcca catgaagaat aaatggataa
      421 atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc cgtctttttc
      481 catcgtataa agaaggatta acggcagatc aagtcaagga acgaatagaa aaaggtgcag
      541 caaataattc agtcgatccc acattcaaaa caaatcagca aatcgtattg gaaaacatat
      601 ttacgtattt caatttgatc tttcttatct tagccatttt gctttgttta gtagaatcct
      661 ataagaactt gactttttta ccagttatca tagccaatac aggaatcgcc atttttcagg
      721 aaattcgatc caaaaagatc ttggacgaac taaatgtttt aaatacagca aaagtcaatg
      781 tcatacgaga cgggatagaa caaacaatag atatagaaga ccttgtaata gatgacattg
      841 ttattttaga gactggatat caaattccag cagatgcaga ggtcgtggaa gggaaactgc
      901 aagttaatga ggctttattg acaggcgaag cagatgaaat aaccaaagaa ataggtgatc
      961 cattaatgtc cggcagtttc attgtttccg gaaaagctta cgcccgatta gacaaagttg
     1021 gagcagattc gtatatttcc cagctgacag tcaaagccaa gacaatggga gaaggagaac
     1081 agtcggagat gattgcttct cttaatcaat tgatcaaatg gattgggatc atcattattc
     1141 ctatcggtat cctgcttttt tctcaaagtt acttttttaa tggaaacacg atcaaagaaa
     1201 gtgtcgttgc aatggaagca gcattgatcg gtatgatacc tgaaggactg tacttgctga
     1261 cgacgattgc attagcatta agtgcgacta aactagcaaa gcaaagagtt cttttacaca
     1321 atatgaaaag cattgagaca ttggcaaggg taaatgtcct ttgtgtagat aaaacaggaa
     1381 cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt ctctaaaaaa caagaaataa
     1441 tcagtcagtc agatcaattg gaagcatgca ttgctgacta tgcaaaagcg atggcaggag
     1501 acaatgcaac aatggaagct gttaaagcat attttacaaa aaccactgat caatcctatc
     1561 accatgttat tcctttttct tctgtccaaa aattcagcag tgtagcgttg gcagacaagg
     1621 tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca attgccggaa tatgcggaag
     1681 agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg tacgatggga
     1741 ttcttgaaga accagtattg acagaagcag tcacaccatt aggctatatt ctgattgcta
     1801 atcctatcag aaaagaagca agagccacat ttgattactt caaaaaacag caagtagcca
     1861 tcaaagtgat ttctggagat aatccgctca ccgtatccaa tgtagctgtc caagcaggga
     1921 tttcacatgc tgatcagtac gttgatgcat ctaaattatc agaagaagaa tatgaagcag
     1981 ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga acaaaagaaa atcttcgttc
     2041 aattgataaa gcgtaacaat acagtagcta tgacaggtga tggcgtgaat gatattctag
     2101 ccatgaaaga agcagactgc agtatagcga tggcttccgg aaatgaagcc gccatgcagg
     2161 cctcacaagt ggtcttactt gattctgact tttcacgcat gccagaagtt gttgcagaag
     2221 gacggcgcgt tgtgaacaat attgagcgat cagccagctt gtttttagtg aagaatattt
     2281 tctccttttt gctctcactg ttttcagtga tctttgctct gacctatcct ttagaaccat
     2341 cacaaatcac attgattagt ttgtttacta tcggacttcc atctttcttg cttgctctag
     2401 aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa aaagctgtgc
     2461 caggtggact aacagatatg atcgtagtcg gtgcgttggt gatctgcggg gtgactttga
     2521 atctaaataa aacagatgtt tctacagcat ctaccatgct tttgattgca gtagggttcc
     2581 tcgttctcta taaaatctgt tcaccgctga ataaattcag aagtcagatc attcttttct
     2641 gtgctagcgg taactttttt tcggtcatct ttttgcataa gctgttttca atcaccggta
     2701 tctcagctgt atcacttttg ctgttgagca ttctattttt ctcggcagat tcgattttta
     2761 ggcatttgac cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa gagcaaaagc
     2821 cgtttagtcg cgtagctaaa aaatttctat cgatatttaa acgagaaaat tgacgaaaag
     2881 tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc aggatgagtc
     2941 atccaacttt aaaacgcacg agcagttgct cttttttaca ttttcggaat ggtaacggtg
     3001 aaagtcgaac ctgttccaag atgactgtct acagtaatcg taccattaag gagttgcacg
     3061 tagtctttta caatcgctaa ccctaatccg gtcccgcctg aatgtcggct tcgcgccttg
     3121 tctacgcggt aaaaacgctc aaagatccgt tcctgatcct caatgtcgat acctatacca
     3181 aagtcttgga cagaaaacga tagatcatct gttgcctgat agcggataat aatctcgctg
     3241 tcgctttttg aatattggac agcattttca atcagatttt tgataattgg ataaaggata
     3301 tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa ccgtttcttt
     3361 tcgatgattg gctgataatt ttgaacgatt tgctggaaat aaggttctgt ataaattgtc
     3421 tggatctcgt aagaattctc gccatctttt gacagctgga tgatttcccg aatcagttta
     3481 tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt ttgcgggtca
     3541 tcttttgcac catccagtaa agtctcggta aaaccaatca gtgaagtcac aggagttttt
     3601 aattcgtgag agacgttgcc aacgaaatcc ttttgtagct tttctaaacg tcgcactttc
     3661 gttaagtcat acgccacccc tagaatctgt ccggtacctt ctgaattagt aaagtagcgc
     3721 aaactcaagt ctaatgtctg ccgaccttct gtaagggtga tctcctgatg gatcagcgga
     3781 tgctcaggag tcacttgatg aatcagctga atgagttttg gctcttgaat gacttctgtg
     3841 taccgctgtt caggttgata gcttaccacg tttaaatgta gctgcatctt tggattaagc
     3901 agtagtaaac gagaatcagc atcatcaatc aggaatacgc caatcatcag ttcgttcagt
     3961 aacgtatata attgctcttc tgtggtagta cacgcccgat acatcttact catttgttcg
     4021 ctcaacgcat taatcgtctg atacaattct tcccattgat cgggtgtttg catgattact
     4081 tccgcctgat taggattttg caccattttt tttagtacgg gcagcacggt tttcaatggc
     4141 tggttttttt gatagatcag ataatagatc attcctgcaa gaacaaggaa gaaagacaga
     4201 aagaagaaaa agacccaacg gcgaaaactc tcagttctcg gtaaaaaaac gcttgtcggt
     4261 tctgcgatac gtaaaatggc ttctaactgg ccactttttt tgactggtaa tgcgacatat
     4321 aaaagatcct cattcaaagt agtacttttg cgcaaagcgc tacctaggtt cccaccattc
     4381 aatacggctt tgatttcggg acgggagttt cgctgttcat tcaatgtttc attgtttgtg
     4441 tcaaataaga tctttccagt agcatccatt aaagtaatcc gttcgttcga ctgatggacg
     4501 aattcttcca aagcagtttt gttttgcgct gcttggaggt tttcgacatc taattgccgg
     4561 atcagcagtt gtgctttttt tgtcaggtag ctttcttgct gttccacgat ctgctgttga
     4621 aaataatggg aaatcaattg ccagccacca aaaaacaaac taactaaaag aataagaaag
     4681 aagagacgtt cataaatttt tttccatttc atcgtttggg ttcctgaaac ttatagccaa
     4741 atcctctaat agtcaccaag tactttgggt gttttggatc tcgctcgatt ttttctcgca
     4801 aatgactaac gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa ttccaaatgc
     4861 ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa taaacaagta
     4921 attcaaattc tttcggtgtt agttcaatca gatggtcgtc tacggtcact tgataattgg
     4981 tcaaatcagc agtgatttgt ccaatcgata gatattcagg ctcagcttca tcttttgatt
     5041 gcttacgagg ttcaattcgt ctgaatattg ctttcatacg ggctaatact tcgcgaggac
     5101 taaatggttt ggtcaaatag tcgtcagcac ctatttctag gccaatgatc cgatccactt
     5161 ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt tttttcttga cgcagccgct
     5221 gagtgatctc catgccatcc atcgttggaa gcatcacatc taaaatgatg aaatcaaatg
     5281 attgttctaa tgctaattct aatccttcgc ctccatctgt ggcactggtt accttgtagc
     5341 cttctttttc caaattgaac gtcaagagcg ttacaataga aggttcatca tcaacaacta
     5401 gtattttttt cattcaaatc tcctttaaat aggtttcatt ctgtttatat tttagcatag
     5461 aatcagtaca aagggctatt ttacaaaaga aataaatggt ataataggaa ggcactttgc
     5521 tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc atgtttaggc ggtctggcct
     5581 gtcgctatga tggacagaaa aaagaagtac cagaattgat ggaactagtt gaaactggag
     5641 aagctgtcat ggtctgtcca gaagtgatcg gcggattacc aattcctaga gatcccgctg
     5701 aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta tggacgacaa
     5761 gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat caaaaattgc
     5821 aggaaaaaca cattgatcta gtcatcatga aggaaaaaag cccttcatgc ggaacccatt
     5881 cgatttatga cggaagtttt tccggaataa aaaaaaatgg tgccggagtg gctactgctt
     5941 attttattca gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa actagaggtg
     6001 agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt ttattcgacc
     6061 ttattgacag aaaaacaaat gaactacatg gaattatatt atgcagatga tttttcatta
     6121 ggtgagatcg cggaagaata cgaagtaagc cgacaagcag tatatgacaa tatcaagcgg
     6181 acaagcaaga tcttggagga ttatgaaaaa aaactccatc ttttttctaa ttatattgtt
     6241 cgagaacagg tattggaaaa tatgactgca tatattacag ataagtaccc ggaagataaa
     6301 aaactacttg aatatgtaca gcaaattcaa gcaatcgaag aataaataaa aggaatgaag
     6361 aaaatatggc atttgaaagc ttaactgaac gtctccagca ggcgatggga aaaataagaa
     6421 aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc cgccttgcct
     6481 tactagaagc cgatgttaac ttgcaagtag tcaaagattt tactaagcgc gtacgcgaac
     6541 gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc gtaaaaatcg
     6601 ttgatgaaga attgacgatc acacttggtt ctgaaacagc agagttaaat aaatcaccaa
     6661 aaataccaac tgttatcatg atggctggtc ttcaaggggc aggtaaaacg acctttactg
     6721 gtaaattagc caattattta aagaaaaatg aaaatgctcg tccgctccta atcgcagggg
     6781 acgtatatcg accagctgcg atcgaccaat tgaaagtatt aggtcaacag ctagacgttc
     6841 ctgtatttga tatgggaact gaagtcagcc cggtagagat cgtccgtcaa gggatggagt
     6901 tagcaaaaga aaagaaaaac gactatgtat tgatcgatac ggcaggacgt ctgcatatcg
     6961 atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca aatgagattt
     7021 tgcttgttgt cgatgccatg actggtcaag atgccgtcaa cgtagctgat agcttcaacc
     7081 agcaattagg tattacaggg gtcgtcatca ctaaacttga tggggatact cgaggcgggg
     7141 cagcactatc gatccgctca gtgacaggcg caccaatcaa attcatcggt tctggtgaaa
     7201 aattgactga cttagaagta ttccacccag atcgtatggc tagccgtatc cttggtatgg
     7261 gggatatgct gacattgatt gaaaaagcgc agcaagacta cgacgagaaa aaagcggaag
     7321 aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt gaacagcttg
     7381 atcaagtgat gggtatgggc ccactggaag atctaatcaa aatgatccca ggaatgaatc
     7441 aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc aaaaaagcga
     7501 tggtttattc tatgacgcca gcagaaagag caaatcctga tctgttgaac ccaagccgtc
     7561 gtcgccgaat tgctgccggt tctggaaaca gtgttgttga agtgaatcga atgatcaaac
     7621 aattcaagga atcaagaaaa atgatgcagc aaatgtccaa aggggatatg aacatcccag
     7681 gtatggacca aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg gcgatgaatc
     7741 gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa aaaaacagct
     7801 tcggtacaga ctgaaaatgt ctactgccgg agcttttcta tcagcaattt ttattcttta
     7861 ctaatacaat tactctaaac gaagaggtgg cacagatgag atgtccgtgg agtgaacaaa
     7921 cagaaagtat gcggcaatat catgatactg tctggggtgt tcctgaatat gatgaccaaa
     7981 agctgttccg aaaattgatg cttgatatta atcaagccgg attgagctgg cagacgattt
     8041 taaataaaag tgaagcattc gatgaagcat atgatcgatt cgatatcaaa aaagtcgcaa
     8101 attatggaga aacaaaaatc agtgaactaa tggaaaacaa aggaattatt cgtaaccgaa
     8161 gaaaaatcga agcagccgtc caaaacgcca aagcagtctt gaaaattcaa gaagaaatcg
     8221 gcagtttttc agagtatctt tggtcgtttt ctaatggaaa aatcgttgat tcttcttatc
     8281 attcacaaga agaagtccca acaacaagtc ctctttcaga tgaattagca aaagatctaa
     8341 agaaaagagg atttaaattt atcggaagca caacaatcta tgctttttta gaagctgtcg
     8401 ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga agaggtcgta cgtatcggca
     8461 gaaagggcga ataaagcatg cgaaaaatca tattttatgg tgctataagt ttagatggct
     8521 atttggcaac gaaaacggat gatcttcaat ggctttttga cactccaaca ggtaagcaaa
     8581 ctacttatga agctttttat gaaacaatcg atacgaccat catgggcaga aaaacttatc
     8641 aggaagcaaa aaaatatttg gagaccgaag caatttatcc agagaaaaag aatttcgttt
     8701 tttccaccca cgcacaatta caattgccag atgctgaggt cgtaaacgaa gatcctgttg
     8761 cttttctaaa aaatctacaa atgacagaag gaaaagatat ctgggttgtg ggtggcggtc
     8821 gattgatcaa accgctattg gaacatcaaa tgattgatga atggtatatc cagattaccc
     8881 ctgtattatt aggggacggg attcctttat tccaaaaagg aacatatgaa gcgcgttttg
     8941 aaatgctaga cacgacacga ttcggtgaat ttatcgaatt gcattatgta cgaaagtaga
     9001 agtaaataaa ataaaaaagg caactatatg cagaaatgtc ctgtacatag ttgccttttt
     9061 tattaccgtt atgcttttta ctctgttaat ttgttagaaa aatctagata cgcatctaaa
     9121 atcgtttgga agaaacttaa tgttccttca acgatattac cattttcatc aattagattc
     9181 gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg gcacattcaa aaagacaagt
     9241 gattggcgta gatggtggtt tgcgccaaag ccgccgatcc cacctggtga tgcagtaacg
     9301 actaaacctg gtttatgatc ccaaacactg tgtccataag gtcttgagcc tacatctaaa
     9361 gcatttttca atactgctgg cacagaacgg ttgtattcag gagtaacgaa aacgacaccg
     9421 tctagtcccc tcacttcctc acgaaaacga gtccattcag caggagcctg ttctggagtt
     9481 tcgagatcct cattgtaaaa tggcagatca tcgattttta caaatacact ttcgtaccct
     9541 tcaggaagca atttcgcaaa agcttctgct acttttttgt tgtaagaatc ttttcttaaa
     9601 ctaccaataa aaaatccaat tttttttgtc atacgatttt tcctccttca aactatctat
     9661 aataatctta caaaagataa gtaactggag caaataaaaa cacttactga caaaagctac
     9721 atagaagata gattacttat ttttaacaaa aaagaagtct aaaatcttct attttctaaa
     9781 aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa aaaaaactgt
     9841 tacactacat cttgtgaata aaacaatgga ggtggaaata aataatggca gttaaaatcc
     9901 gtttaaaacg tatgggttct aaaaagagtc cgttttaccg tatcgtcgta gctgattcac
     9961 gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct ttgaaagacc
    10021 ctgcagaagt agttttaaaa gaagacttag ttcttgactg gttatcaaaa ggtgcacaac
    10081 cttctgacac tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa caccacgaag
    10141 ctaaattctc aaagaaataa ggtgaccgga tatgaaagat ttaagcgact tagtcttaac
    10201 aatcgttcgt ccgctagtca cttatcctga tcaagtcaag ttagacgtcg tagagtcgaa
    10261 agacttttac gagtacaatc taacagtggc tcctgaagat attggccgta tcattggaaa
    10321 acaagggcgt gttgctaaag caatccgcac aatcgtttac ggtgttcgga tcaatgcacc
    10381 gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag taaataaacg tgctgtactc
    10441 gaattttcga gtgcagcttt tttgtttttt tattaattga agaaatgatt atttggcaaa
    10501 acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga gaaagaaaat
    10561 gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc aagaatcaga
    10621 caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa aaagtagtac
    10681 caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga aaaagactgc
    10741 tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg taccagtctc
    10801 ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta ttttatatta
    10861 cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc
    10921 aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc
    10981 atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca cagggtatca
    11041 gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc gtattcgaat
    11101 ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa tcgtagctta
    11161 tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg ttgatatggg
    11221 tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat
    11281 cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa
    11341 tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa tcaaaaatag
    11401 cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag aattgcctct
    11461 tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg actgaatact
    11521 tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt gttatctcaa
    11581 caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta ttccaagacg
    11641 gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat tttgacttat
    11701 tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat ggcatattaa
    11761 aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac catgagatca
    11821 tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa atcttgtcac
    11881 caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata ttgcttccat
    11941 atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt gtagaaattc
    12001 cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag aatgtttgaa
    12061 ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt agatatcaat
    12121 atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga ttatccatac
    12181 ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat ccaagccatt
    12241 gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc tggtaaacga
    12301 ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt catttgtggt
    12361 cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt ttccttagga
    12421 gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc aacagttcgg
    12481 cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta ttcaaccggt
    12541 ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa agtaccagaa
    12601 gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga atcactaaga
    12661 aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc tcaaatgacg
    12721 aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga agccaagtga
    12781 ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta tggtgagtgc
    12841 aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa tatttggaat
    12901 aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca attacgttct
    12961 gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt
    13021 actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg tgctggaatc
    13081 agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg tacattccca
    13141 ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca
    13201 aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga aatcagaaga
    13261 taaacacgga aaacttcctt ggaaatctga aatttgtgaa aagtccttgt atatcaaggg
    13321 ctttttcttt tttctctatc acagttttaa gccatttttg gctgttttgg accatttttc
    13381 aatcatattc ccttaaaaat tgtcctagat tgtccgacat tgactatcag tgtcgtcaaa
    13441 atagggcaaa atttagtagt cagcttgacg acaaattata atacgaaaaa taccaaaaca
    13501 tttaccgaat ttagcaactt ttgaacccaa gtttctcaaa gggtcagcta ttagcaaaaa
    13561 agacaaaaca tgtacttttc tactaaaaag ttgatgtgat aggcttctat ctaaataact
    13621 attaggagtt tttatcgtga agcaaaatga tggacgtatc atttggaaaa atcaaagtga
    13681 gttaaaactg attttaacaa ttaatgaatt catcgaaaaa cacggaatta catctagccg
    13741 tcaataccag aaaaagctat cagaaaatcc aaattctgca ccaagtatgt ggttcattaa
    13801 caaaaaatat ggatcatggg agaatttatt gattagtata ggacgagaaa atactgggta
    13861 tggaaaatgg gctagaatgt cagaacaaga attattggaa atagtagaag cctttattaa
    13921 gtgcgagaag ataacctctc aacgaatgta cgaacaaaaa tcagtaggga agaatattcc
    13981 ttcacttagt accattaaaa agatgttagg agatatacgt ccacttttta aagaaaaaaa
    14041 tgatggatct cgatttactg atttcgaact gctgttagaa ctaaaaaatg aaataatccg
    14101 attaaaacta caggatgatt tatcaatgac aaagtttcga aaactagttc aatccccaaa
    14161 actaccatca gttgatacaa ttatgaaaag aacgaacaaa aattgggagg aattgatggc
    14221 agagattgga tttgattatc gaagaatcaa aatttataag cagagaaaca acctatccaa
    14281 gacaaagaaa actaaatagt atcactctaa atcagacgtt caaaattaaa atttggacgt
    14341 cttttttatt acccaaaaac gaaaggagat ttattttatg aaaaagccaa gatttaagaa
    14401 ttggcgtttg ctcgcaacac tgagtctatt atgccaaaca attggtggat cgcttggccc
    14461 aacgattgcc tttgctgatg aaattactca tccacaaacg gtaactgtgg aattggattt
    14521 ggctcaccag tatgctgtcg aaggtacatt tagcgatggt cgtcccatgt cagaagttac
    14581 cgttccgcac tatgcagttt acaatggtgt gaagcaagat attttctgta ttgagccagg
    14641 ggtgccgatt tacaatgagt tcactcctgg ttatgagaaa aatccattgc ctgatatgtc
    14701 ggagaaagcg aaattagttt ccgttttatg gaaggaagct ggtacggatg tggatacaca
    14761 tattgtggcc cagaagatga tttggcaaga agttaatggc tacacgcttc actcaattaa
    14821 acgttctgat ggtagtgcag taaatatcgc agccattgag tcaaaaatca atcaagccat
    14881 tgcggattat caaaagaaac caagcttcca taactccaca gtgaaaaccg tgttaggtca
    14941 atcgactact gtgacagata cgaacggctt gaatttatcg gagtttgatg aagttgtcga
    15001 gaatatcgca aatattgatt atcgtgtgaa cggcaatcaa ctagtgatta ctccaaatgc
    15061 caattcaaaa gagagtggtg aattaaccct aaagaagtca gctaatacag gaacaccggt
    15121 agcttataaa atggctggtc aacaaacttt gatggcgggg gcaattgata agcccaatac
    15181 ctacacaatc aaaattgatg tggaaactga gggttctttg aagattaaaa aagtcgataa
    15241 agaaacagga aatgttgtac cgggaaccgt tttccattta gactttggaa aaacattacc
    15301 tgcaaaagac gtgaccaccg ataaagaagg cattgcgaca ttggatgaga ttcctcatgg
    15361 cgcgaaagta accattactg aaaaatcagt gccagctcct tatacgattg acactacacc
    15421 aatcaccgca accattaaat ctggtgagac tatttctgtg acttcaaaaa atactcgcga
    15481 aaaaggtcaa atcattttga ataaaatggg ggtggaaaca ggaactgatc tttggaacga
    15541 caaatactca ttagccggaa atacgtttgc cattcgcaaa gacagtccaa ccggtgaaat
    15601 tgtccaagaa atgaccacgg atgaaaaagg tcatgcggaa acaccaaaag agattgccaa
    15661 tgcgttggaa ttgggaacct attatgtgac cgaaaccaaa gctagtaatg gctttgtgaa
    15721 tacctttaaa ccagtaaaag tcgaattgaa ctatgtcaat caaaccgtgg ctcttgttac
    15781 cagtaacgta aaagggcaaa accaagaaat cactggtgaa accactttga caaaagaaga
    15841 caaagatacc ggtaatgaaa ctcaaggtaa agctgagttt aaaggagctg aatatactct
    15901 ctttactgca aaagatggtc aagcggtcaa atggagtgag gcttttaaac cagagttagt
    15961 gaagggaacg aaagcttctg atgaaacggt gactttgact ttagatgaaa agaatcaagt
    16021 tgctgttaaa cacttagcga ttaatgagta ttactggcaa gaaaccaaag cacctgaagg
    16081 atataccttg gatgaaacaa agtaccctgt ttccatcaaa aaggtggatg ataacgaaaa
    16141 aaatgccgtg attacccgag atgttacggc aaaagaacaa gtcattcgct ttggctttga
    16201 tttctttaaa tttgctggat cagctgctgg gactgccgaa actggcttca atgatctgac
    16261 ctttaaagta tcgccattgg aaggaaccag tgagattaca ggtgccgaag atgaagcaat
    16321 tacagcttat aacgagcaac taggttttga tggctatggc aagtttgaaa atcttcctta
    16381 tggggattat ttacttgaag aagtagaggc tccagaagga tttcaaaaaa ttacgccatt
    16441 agaaatccgt tctactttta aagaaaataa agcggattac actaagagtg aatacatttt
    16501 tacgattacc gaaaaaggac aaaaacaacc aattaaaacg gtgaccgttc cttacgagaa
    16561 actgacgaat aaagcatttt ctgttagctt gaatcgtttg atgctctatg atttacctga
    16621 agaagaagat agcttaactt ctcttgggac ttggaaagac ggaggcaaag aattgacaga
    16681 ccttgattct accgagctag ttgataaatt gagctataac ttgcatgaaa tcaaagaaga
    16741 ctggtatgtg gtagcgcaag ccattgatgt ggaagcgaca aaagctgccc aagaaaaaga
    16801 tgaaaaagcc aaaccagtgg taattgccga aacaaccgca acgttggcga ataaagagaa
    16861 aacaggaact tggaaaattt tgcataaatt aaccgcagaa caagttttgg ataaaaccat
    16921 cgtcttattc aactatgtgt atgaaaacaa gaaaccctat gaagcaggtg atgatccagt
    16981 agcgaaagat gctagcttga acaatcaagc ccaaactgtc aattgtacgg tggaacgcca
    17041 tgtttccatc caaacaaaag cccacctaga agatggttcg caaaccttta ctcatgggga
    17101 tgtggtggat atgttcgatg atgtgtcgat tacccatgat gtactggatg gctcaaaaga
    17161 agcttttgaa acaattctgt atgcattact tccggataat acgaccaaag aaatttggaa
    17221 atctggcaaa attgattatg aagtgaatga caaagaattc actaaaaccg tacttgcgga
    17281 aaaagtggac acaagtaaat atccagaagg tacaagtttt accttcaaag aaattaacta
    17341 tggcaaagac ggaaacatca atgggaaaca caatgaggat ctaaaagaaa aaactcaaac
    17401 tttaacacct aaaaaagttc cgacaacacc aagtgcacca gaacaaccgg aaataccaac
    17461 tgttccaagt gactctcaag aatctagtcc tacagtaaag acattcccac aaacagggga
    17521 gaaaaattct aatgttctgc tgtttattgg gtttaccttg atctttgcga ctgctgggta
    17581 ttatttctgg aatcgccgaa actaaggtga tgtgatgaaa caactaaata ggaaaaacca
    17641 acgtgcgcga ccgcctccta aaaaaagtgg tcgcgctatt tttgtatcca aaactaaaaa
    17701 tgaaaagagg aaaagaagat ggaattgaaa tttgttgtgc cagatatggc cgaaaccttt
    17761 ggaaagataa ggtatgccgg cgaaggcgaa gttttaactg aaggatatgg gcggaatact
    17821 acagtaattg gtcgtagtta tcatttgtat tccagtaagc aacgagccga tgatattgaa
    17881 gtggtggtag ctgcggaagc cggcgaaaag gattttgatc aagatcaacc actaaaagcc
    17941 gtgaatcccc atttggttgc caaaggctat gagattgaga atcgtgggtt taccgactac
    18001 gtgttgtatg tcgatgattt agtgaaagca taggaggaaa agaagatgag attaacagaa
    18061 ggtattgttg tagattcagg actaacgttt gggaaattac gattttccgc attacgccga
    18121 gaagtacgga agcaaaatga ggatggaacg gttagtaacg aggtaaaaga acggacttat
    18181 aatttgaaat cctctgcgca aggtcgaatg atccaagtta gtatcccagc aaatgtaccc
    18241 ttacgcgagt ttgcctatga cgcagaggta gagttggtca atccaatcat ggatacggtc
    18301 gccaattatg ttttccgaga aggaactact gtcaattggt tcattaaggc cgatgattta
    18361 gtcttgaaac gacaaccaga tcagggaaat tctacaaacc aaaatgaagg aaagaaatag
    18421 gtgaagcgta tggaaattaa tgttgaacat attttagatt gtctcgatca atatggaaaa
    18481 ggtgagctaa cggaggaaca actcactaaa gcattaacct atgatgaaaa aatgtttctg
    18541 attatgcatc aagggttact ggaagtaggt aacgatgccg aagaagattt tgatgtactg
    18601 aattggttag gggaagaaga gtccttcttt atggttatcg aagtaaatga aagtctatgt
    18661 cgagaagccg aaaccgtatt ggaagaaatt ggtgtggaaa tgccggatgc cattgaagtt
    18721 tttttaaagc aactagtgga aacgaaacaa ctccctatag cagtaaatga ctagcataac
    18781 tgcttgaaat tggatcaatg aaatcctatg aagctttttt cgattggagg tggaaaccat
    18841 aaaaatgtac aaagggcatc gtatacgggc aggagatcag cacttggttt atcactttgt
    18901 tcttggctgg ctactcgctc tgttcatcgg ttggatgagc gtcttttatt ttcaagagct
    18961 tagacaattt gatatttcta agctatcact ttctactata gaaatcgttc gttctataaa
    19021 agatttaatc tatttgctag gaagtctcgt tctttcagga agcacgatgt tgctttatat
    19081 acactttttt caggatcatt ggcgcagttt gtggcatcga caaaagctag cccggatgat
    19141 tctagaaaat cattggtacg aagtaaaaca aactcagagt gaaggatttt tcaaagacct
    19201 caatagtagt cgaaccaaag agacaatcag ttacttcccc aaaatctatt accgtatgaa
    19261 ggatggttta ctttctattc gcgttcaaat ttcactggga aaatatcaag atcagctttt
    19321 aaagctggaa aagaagttag aaagtggtct atattgtgaa ctagtagaaa aggaactcaa
    19381 agactcctat gtggagtaca ctttactgta tgatataatt gccaatcgaa ttgggataga
    19441 cgaagtagtg gcagaaagtg gggctttgcg attgatgaaa aatcaagtat gggcttatga
    19501 ttctttacct catatgttaa tcgctggcgg cacaggtggt gggaagactt actttttact
    19561 cactatcatc gaggctttat taaaatctga tgccgagctt tttgttcttg atcccaaaaa
    19621 tgcggactta gccgatttag gtacggtaat gcctcatgtc tattcccaaa aggaggaaat
    19681 ttctgcttgt gtggaagatt tctatgagcg catgatgact cgtagcaagg caatgaaaga
    19741 aatgcccaac tacaaaacag gagagaatta tgcgtttctt ggacttccac caaacttttt
    19801 aatctttgat gaatacgtgg tttatatgga aatgttaacg acgaaggaaa gtgcggtgat
    19861 tttgaataag ctcaaacaaa tcgtgatgtt aggtcgtcaa tctggtttct ttctgattct
    19921 agcttgtcaa agaccggatg cgaaatattt aggagacggg attcgcgatc aatttaattt
    19981 ccgggtggct ttgggtcgta tgagtgaact gggatattcc atgatgtttg gcgaggttga
    20041 taaaaaattc tttatgaagc gtatcaaagg tcgaggctat gtagatacgg gagggagtgt
    20101 gattagtgag ttttatacgc cacttgtacc aaaaggatat gatttcttaa gggaaattag
    20161 cgacattggt ggattaaacg ttaatacgga aggataaaat aatagtatgt agcctaggag
    20221 tgtcaagaat cctgggcttt ttttacgagg tgatttatga catttttaga tttattcgct
    20281 gggattggtg gcttttgctt aggtatggaa caagcgggtc accagtgtat cggcttttgt
    20341 gaaattgatg attttgctcg ccagagttat aaggcaatcc atgatacaag taaggaggtg
    20401 gaaatgcatg acatcacaag tgtatcagac gagtttattc aatctctcgg accagtggat
    20461 atcctttgcg gtggatttcc gtgccaaact ttttcaattg cgggaaagcg gcaaggattt
    20521 tccgatactc gaggtacttt attctttgaa atcgctcggt tcgccgctat tctccaacct
    20581 aagtttttat tccttgagaa cgtccgggga ttactcaatc acgaaggagg ggctacgttc
    20641 gagacgatcc tccgaacgct ggatggattg gggtatgatg tggaatggca agtgcttaac
    20701 tcaaaggcct acgttcccca aaaccgtgag cggatcttcc ttatcggaca ttctcgagac
    20761 gcatgtaccg aacaagtatt tcctatcatt ggatcgtctc caacatctga tcaaaacatc
    20821 agaaacttat taaatatcaa cccttccaat cgaggaatgg ggggacaagt ctatgggtca
    20881 gatgacgtag cgcctacctt gaccggtgat gaaggaatca aaatcgcatt gccggtagat
    20941 gatggaattt ctgtagcggg tatgttactg ggaaattttg aacaaggcaa tcgggtttat
    21001 gagataactg gaacagcgcc tactttgtca acgaaacaag gcggaacgaa gatcatgctt
    21061 cggaaaaacg aaacagaata tcaagaggtg aagccaggga acagtgtgaa cctggctttt
    21121 cctaattcca caagtcgtcg aggccgtctg ggaaaacaaa gtgtgcatac cttattaaca
    21181 ggagaccaac aagcagtcgt cacggatcag tatcaaattc gaaagttaac acctagggag
    21241 tgctggcggc ttcagggctt tccggactgg gcttttgatc gagcttctca agtcaattca
    21301 gatagtcagt tgtataagca ggctggaaat tctgtaacag ttccggtcat ttttgatatt
    21361 gctagaagat taaaggaggt aaaaacagat gatttatgat gagttgattg gggaatctac
    21421 tgggtgattg gcaagattca gtcagatccg gaattggaag aagaactgta ccggctcaat
    21481 tttgatattc gcaaaaacgg ggtgaaagtc cctgatgatc cgtatgtaat tgatgaggaa
    21541 acggatgctc gaatggagct gaatcaagtc atcagtgaat tggaacgaat tgcggacttt
    21601 gctaaagaac ctgatatccg ccaatacttg tttgagataa aagcagagtt ggaaattgag
    21661 ggtattaccg cggagtaacg gccaaataaa ccactgatag gcactgaccg cgggggattt
    21721 gcgaagcaga aatcctccgt ggtgctgtgc ctgtttatgg acggcgttag ccggccatga
    21781 tgtctcaccc cccgtatcta acaggggggt acaaaaacac aaaaaataag caagcaacga
    21841 taaggaatgg ctgtcctatc aaggataata agcaatttgt gtagatgtta actgatttga
    21901 aaaagttaac accttaaatt tggaggtgag aacttggcac aaaggaattt agattatcgg
    21961 ctattaaaag accggcgtaa tgaatatggc gtttcacaaa ataaattagc tacgacttgt
    22021 ggacttagtc gcccgtactt aaatcagata gaaaatggtg gcgtaaccgc atcgacaaaa
    22081 accatgagga aaatttttga ccaactggaa agtttcaatc ccgagttacc tttaacgtta
    22141 ctgtttgact atgtaaggat tcgctttccc acaacggatg cgaggaaaat cattcaagag
    22201 attctccatt tgaaatttga ttatatgctc cacgaagatt acgcctttta ctcctatcaa
    22261 gaacaatatg ttatgggaga tattgtggtg atgttgtcac atgaagaaga taaaggcgtc
    22321 cttttagaat taaagggtcg tggttgtcgg cagtttgaaa cttttttact cgcccaaaag
    22381 cgtagctggt atgacttttt cgaagattgt ctaaaagccg gtggcgtgat gaaacgctta
    22441 gacctggcaa tcaatgatcg agttggcctg ttagatattc ctgatttaac gaaaaaatgt
    22501 cagaaggaag aatgtatctc cttgtttcgt accttcaaaa gctatcgttc cggggaactg
    22561 ttgaaacctg atgataaaga tggtatgggc aatactttgt atattggcag tcttaagagt
    22621 gaggtctatt tctgtttgta cgaaaaagac tatgagcaat atatcaagtt agggattcct
    22681 ttagaccaga ccgaaacgaa aaatcgtttt gaaatccgac tgaaaaatga tcgagcttat
    22741 catgcaattc aagatttgtt aaaaggtcgc agtattgaaa gtaccacatt ttccattatt
    22801 aaccggtact tgcgatttgc ggataaggtg gaaggcaaaa ggcgaactaa ctggcccttg
    22861 aatgaacaat gggggcgctt cattggacgg aatcagaagg aaattcaatt aacttctgaa
    22921 ccgaagcctt ataccattga acgaacgttg aactggcttg gacgacaagt cgcccctacg
    22981 tggaagatgg ccaaggagtt ggatcggttg aatcagacaa cgtacattca ggatatggta
    23041 aataatactc gactatcaga ccggcacaaa aagattttgg agcaacaaag tatggcaatt
    23101 gaaaatttga ttatatgaaa agaggaatcg tgatggaaat tgtgaaagat attttattgg
    23161 tgtttggtgg cagtatgctt ggtgttactg tgatgtgctt gatgcatgca agtgcagcgg
    23221 cggaccgagc aatggaaaag aaaggaaagt gatgaaatga atttcggtca aaattt
//
LOCUS       DAFEHS010000043        22453 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFEHS010000043
VERSION     DAFEHS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.044"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            6..305
                     /locus_tag="LP327_001704"
     CDS             6..305
                     /locus_tag="LP327_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1725819.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LP327_001705"
     CDS             378..1739
                     /locus_tag="LP327_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL1725820.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LP327_001706"
     CDS             1761..2021
                     /locus_tag="LP327_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725821.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LP327_001707"
     CDS             2035..2787
                     /locus_tag="LP327_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBL1725822.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LP327_001708"
     CDS             complement(2881..3486)
                     /locus_tag="LP327_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL1725823.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LP327_001709"
     CDS             complement(3539..4513)
                     /locus_tag="LP327_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBL1725824.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LP327_001710"
     CDS             complement(4891..5610)
                     /locus_tag="LP327_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL1725825.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LP327_001711"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LP327_001711"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL1725826.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LP327_001712"
     CDS             7161..8558
                     /locus_tag="LP327_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL1725827.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LP327_001713"
     CDS             8639..9079
                     /locus_tag="LP327_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL1725828.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LP327_001714"
     CDS             9227..9553
                     /locus_tag="LP327_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL1725829.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LP327_001715"
     CDS             9554..10303
                     /locus_tag="LP327_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725830.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LP327_001716"
     CDS             complement(10383..10838)
                     /locus_tag="LP327_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL1725831.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LP327_001717"
     CDS             complement(11265..11540)
                     /locus_tag="LP327_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725832.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LP327_001718"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LP327_001718"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBL1725833.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LP327_001719"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LP327_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL1725834.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="LP327_001720"
     CDS             14770..17436
                     /locus_tag="LP327_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL1725835.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="LP327_001721"
     CDS             17800..18552
                     /locus_tag="LP327_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL1725836.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="LP327_001722"
     CDS             complement(18574..19728)
                     /locus_tag="LP327_001722"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBL1725837.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="LP327_001723"
     CDS             19913..22354
                     /locus_tag="LP327_001723"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBL1725838.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7210 a         3937 c         4675 g         6631 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt aga
//
LOCUS       DAFEHS010000044        21500 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFEHS010000044
VERSION     DAFEHS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.045"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            2..400
                     /locus_tag="LP327_001724"
     CDS             2..400
                     /locus_tag="LP327_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL1725839.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="LP327_001725"
     CDS             384..1079
                     /locus_tag="LP327_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBL1725840.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1663..2550)
                     /locus_tag="LP327_001726"
     CDS             complement(1663..2550)
                     /locus_tag="LP327_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL1725841.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2735..3574
                     /locus_tag="LP327_001727"
     CDS             2735..3574
                     /locus_tag="LP327_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL1725842.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3770..4744
                     /gene="sgrA"
                     /locus_tag="LP327_001728"
     CDS             3770..4744
                     /gene="sgrA"
                     /locus_tag="LP327_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBL1725843.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5042..5398
                     /locus_tag="LP327_001729"
     CDS             5042..5398
                     /locus_tag="LP327_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725844.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5401..6558
                     /locus_tag="LP327_001730"
     CDS             5401..6558
                     /locus_tag="LP327_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1725845.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6584..7024)
                     /locus_tag="LP327_001731"
     CDS             complement(6584..7024)
                     /locus_tag="LP327_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL1725846.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7184..7690
                     /locus_tag="LP327_001732"
     CDS             7184..7690
                     /locus_tag="LP327_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL1725847.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7858..9771
                     /locus_tag="LP327_001733"
     CDS             7858..9771
                     /locus_tag="LP327_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL1725848.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9776..10777
                     /locus_tag="LP327_001734"
     CDS             9776..10777
                     /locus_tag="LP327_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL1725849.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10979..11185)
                     /locus_tag="LP327_001735"
     CDS             complement(10979..11185)
                     /locus_tag="LP327_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBL1725850.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11830..12678)
                     /locus_tag="LP327_001736"
     CDS             complement(11830..12678)
                     /locus_tag="LP327_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL1725851.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12677..13744
                     /gene="gnd"
                     /locus_tag="LP327_001737"
     CDS             12677..13744
                     /gene="gnd"
                     /locus_tag="LP327_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBL1725852.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13746..14747
                     /locus_tag="LP327_001738"
     CDS             13746..14747
                     /locus_tag="LP327_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBL1725853.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14744..15028
                     /locus_tag="LP327_001739"
     CDS             14744..15028
                     /locus_tag="LP327_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBL1725854.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15061..15858
                     /locus_tag="LP327_001740"
     CDS             15061..15858
                     /locus_tag="LP327_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL1725855.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15872..16705
                     /locus_tag="LP327_001741"
     CDS             15872..16705
                     /locus_tag="LP327_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL1725856.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16723..17580
                     /locus_tag="LP327_001742"
     CDS             16723..17580
                     /locus_tag="LP327_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL1725857.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17602..18111
                     /locus_tag="LP327_001743"
     CDS             17602..18111
                     /locus_tag="LP327_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1725858.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18148..18549
                     /locus_tag="LP327_001744"
     CDS             18148..18549
                     /locus_tag="LP327_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725859.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18661..19014
                     /locus_tag="LP327_001745"
     CDS             18661..19014
                     /locus_tag="LP327_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725860.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19048..19491
                     /locus_tag="LP327_001746"
     CDS             19048..19491
                     /locus_tag="LP327_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL1725861.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19508..20593
                     /gene="uxuA"
                     /locus_tag="LP327_001747"
     CDS             19508..20593
                     /gene="uxuA"
                     /locus_tag="LP327_001747"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL1725862.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20586..21299
                     /locus_tag="LP327_001748"
     CDS             20586..21299
                     /locus_tag="LP327_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL1725863.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7397 a         3302 c         4490 g         6311 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa acttttttaa
     1501 attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc
     1561 cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc
     1621 acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga atatattttt
     1681 tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga ttgttttatc
     1741 catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct cagatttgta
     1801 tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat aatagctttg
     1861 ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt tttttagttt
     1921 aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa aattcatttt
     1981 tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct tggagaaatt
     2041 ttccccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg aaaatagaat
     2101 gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat ctttatctgt
     2161 taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa agagtttctt
     2221 atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg gaccgagttt
     2281 tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt ttccgtatac
     2341 atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat ttgatttaac
     2401 ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg ttgcaatcgt
     2461 ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa ggcacataat
     2521 tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag tctaacagaa
     2581 gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt agttcgtaaa
     2641 aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa gaaagaaaag
     2701 ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc ggttgattgg
     2761 atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata atgcacttgc
     2821 ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg tcggacattt
     2881 agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa gaatagaaga
     2941 ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc tcgttgttgg
     3001 aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt atcggttaga
     3061 ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta ttgcagtggg
     3121 ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta gatttggtcc
     3181 gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat tgaatcctaa
     3241 catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca gcgcacttat
     3301 ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga attggacaca
     3361 aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt tctccactct
     3421 gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag catctatatc
     3481 aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat ataaaacaaa
     3541 gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa ggctgatatc
     3601 ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat tcaagcgaat
     3661 acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca ttttcatttt
     3721 atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa tgaagaaaac
     3781 agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga ttggaatcag
     3841 tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa ctgatcggat
     3901 tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca aaagcgtgaa
     3961 tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa gttcagaaaa
     4021 agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg agtatacaat
     4081 aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag ttgatttgat
     4141 tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca gttggacagt
     4201 tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag aaaacacgag
     4261 cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca cgagcagcga
     4321 aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca gcgaaagcag
     4381 tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga gtagcacagg
     4441 aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt tggtacctat
     4501 cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg tgctaaaaca
     4561 gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa cagataatag
     4621 aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta catggatggg
     4681 tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc atcgagtgaa
     4741 ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt gagtgaatat
     4801 ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc tatttttgtt
     4861 tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata agagcaagaa
     4921 taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac gaatctattt
     4981 gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag gagtatgtac
     5041 tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt tgaactattg
     5101 ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc taaactacac
     5161 cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa ggtatccaga
     5221 atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt cttctcagtt
     5281 tttactgagt aaagtttatt ctatactttt agagggaacg attggttata ttttactgga
     5341 ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa ttatataagt
     5401 ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa ctatcattta
     5461 tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat ccgatacttt
     5521 tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt atcaagtata
     5581 aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac gcgtagtata
     5641 gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa acaaggccac
     5701 tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat aacaaaagaa
     5761 gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc gatgaatcac
     5821 atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa cacctatttg
     5881 gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg gattgaatcg
     5941 tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc agaaaaaaat
     6001 tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg gacaaaaaag
     6061 aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat atttccagtg
     6121 atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc agagcttaat
     6181 acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt cagggatatt
     6241 tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg taaaacacag
     6301 gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa atatagtgca
     6361 tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa aaaggcatat
     6421 gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa tgattgggcg
     6481 cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat attggaaaaa
     6541 ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac attgcaaaaa
     6601 gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg agctcttgct
     6661 ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca gtcaaaagtt
     6721 ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta cgaatctcaa
     6781 tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta taactaatag
     6841 aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag tggataaatt
     6901 catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata tcatggacga
     6961 taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac aatttaatca
     7021 tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa atagtcgaaa
     7081 agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa ctagctagat
     7141 aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa acacagccga
     7201 cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc cgatacacga
     7261 attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa cgattgggaa
     7321 accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa aaccatggga
     7381 gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa cccatgcgaa
     7441 ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc attcgatcga
     7501 attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg ctcatggatt
     7561 aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc cttttatgaa
     7621 agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc tgacattttt
     7681 ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt tatccattct
     7741 gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata aaccatttat
     7801 tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata aaaaggagtg
     7861 aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt tgctgtaagt
     7921 acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc ggaatctcgg
     7981 gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag tacttcacaa
     8041 acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga tcaagaaacg
     8101 accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat gtcaaacgac
     8161 gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga tgatatcggt
     8221 aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca gatgacagaa
     8281 atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt agtcactgag
     8341 cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat tgctacttat
     8401 ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct tggaggtaca
     8461 gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa acaacttcat
     8521 caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata ttatgtcgat
     8581 caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg gaataacggg
     8641 atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt atttgttgat
     8701 tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata tgagtcagat
     8761 gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa gattttactt
     8821 tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga tattcctgtc
     8881 aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca actacgtttt
     8941 acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca gttagacatc
     9001 attggggatc aagagcatga agatttattt atgggccttt tgaaacaata ctattttgat
     9061 caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc agattcattg
     9121 cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa cgcgcaaaaa
     9181 ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc agggcatgga
     9241 aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt tttgaaagta
     9301 gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag tctactcaaa
     9361 gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac agagcaaaag
     9421 acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat ttttaatgag
     9481 acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc aaaaaagcaa
     9541 gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga tcaatcaagt
     9601 gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga tgcaaaaaaa
     9661 agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc aggtggattt
     9721 ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta aatgaatgaa
     9781 atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt tagtcactgt
     9841 atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa aacaatttga
     9901 cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa aaatcgaaca
     9961 aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata atatggatat
    10021 ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc atggaggcgc
    10081 atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat gttcagacac
    10141 gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac atcctacacc
    10201 agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag atcaagcaga
    10261 ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg gagaatattt
    10321 gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga ctttggagaa
    10381 caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg cacttactca
    10441 agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt actttgataa
    10501 taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac atgcggatcg
    10561 ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga aacaaatgga
    10621 agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag attatgcgga
    10681 gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac aagcgcaaga
    10741 aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc aaaagaggtt
    10801 gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag cttttcatgg
    10861 tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca ccttttattc
    10921 gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat tttataagtc
    10981 agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg gaggcaagaa
    11041 gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt ccgttgttat
    11101 tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta gcgaaaaaaa
    11161 gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt gtatgataaa
    11221 tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat gatcatctag
    11281 cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat cagataggta
    11341 caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat aatagaaaag
    11401 atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa cttttttatt
    11461 ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta aaatgaggcc
    11521 aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa ttttttagaa
    11581 aataagaaac aaaaataata aaaaaagaaa agataaattt tttactactg tatactaact
    11641 ttgttatcgc atctaacaag taactcgaat aataccaata caaccttctc caaagatgcg
    11701 atgacatacg ttctaaccca ccgactattg atgttctgta ccacccttgc agaaccaaaa
    11761 aagccactac caaatatttg gtagtggctt tttttgtttt atggattttt cgctttttct
    11821 gttatggtgc tattctttga tttcagaaat agtatgtcga actttagcga tagaacgacg
    11881 tgattctttt tcgttgtaaa acatcaaaat cacatataga gagtccaaaa tgcttagttg
    11941 agaaatacgg gaagctaaag cttcagatcg gaattctgtt tcttcagaaa tagaaataaa
    12001 aacgacatcg ccaagtttag caagggaaga attagcttgg ctagtaataa cgattgtttt
    12061 tgctccagtt tttcttacgg tttcagcaat atggattgtt tcttttgatt gccctgtgtg
    12121 agaaatcaaa acagcacagt ctttttccgt caataaagaa gcttccatca attgaatatg
    12181 gtaatccgtg ctgtgagaag ttgcgatagg ggaacgcaaa aatttatgat aagcgtcagt
    12241 tgctacaatc tctgaaccgc caacaccgaa aaaatagaca cgtttagatt cattgataat
    12301 agtagccgct ttttttagat ccttttgttg taacaacttt tttgtgtcat ttaaagttga
    12361 aatactagac tcgaacacct tttgcgccat agtgagctca gtatctgttt tctgaatatt
    12421 ttcgtggata gaaatagcag atagatcatt ttcttggatc aacatggcca ttttaaaatc
    12481 tttaaatcca ttataaccaa gtttttttgt aaattgaaaa aaagtagagt cagcaatgcc
    12541 cagttcactt gctaaattgc taataggact atgacttact ttgcttggat tttctaaaat
    12601 ataatcggcg atagcctgtt cttttgggct gaagtcgttg tacattgttt tgatttgtag
    12661 tcttatggag acgctcatga gaattcctca ttttcataat aagattatca ttttttccgt
    12721 tctcattaat gttaaagata caaggagagt aggaaaaaat caaacaaaaa atatttttgt
    12781 aaaatctatt tacaaaaaaa tttttttgta taatgagggt ataagaaatg gaggcgctat
    12841 catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa
    12901 agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag
    12961 agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa
    13021 aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact
    13081 ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga
    13141 ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac
    13201 ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga
    13261 aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta
    13321 tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg
    13381 aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga
    13441 cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga
    13501 aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc
    13561 tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc
    13621 agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc
    13681 aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga
    13741 gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc
    13801 cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag
    13861 agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg
    13921 ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc
    13981 gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg
    14041 tatctatcat acctgcttta gttgaacgaa atgctgtagc agaaaataat ataactaatt
    14101 tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg
    14161 aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg
    14221 caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc
    14281 tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag
    14341 aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag
    14401 agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat
    14461 ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg
    14521 gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc
    14581 cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat
    14641 gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc
    14701 ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt
    14761 gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc
    14821 aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac
    14881 ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt
    14941 tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat
    15001 catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta
    15061 gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt
    15121 aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg
    15181 aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca attagtgatt
    15241 gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa
    15301 tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa
    15361 gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat
    15421 atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt
    15481 gctggtttag ttggaggatt aggagataaa tacgcaagaa atgtggcagc acatgctatg
    15541 catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta
    15601 gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta
    15661 tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg
    15721 aaagataaac agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat
    15781 ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt
    15841 tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt
    15901 gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt
    15961 gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt
    16021 tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc
    16081 aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt
    16141 attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg
    16201 aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa
    16261 agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt
    16321 tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt
    16381 gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt
    16441 aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga
    16501 ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat
    16561 tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca
    16621 aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga
    16681 agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat
    16741 aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga
    16801 tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta
    16861 tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca
    16921 tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact
    16981 ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc
    17041 ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga
    17101 atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct
    17161 tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat
    17221 caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca
    17281 ggcctaaagc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg
    17341 tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg
    17401 atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta
    17461 cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca
    17521 caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa
    17581 aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg
    17641 atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt
    17701 gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc
    17761 cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa
    17821 tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac
    17881 ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat
    17941 cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa
    18001 ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct
    18061 gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct
    18121 tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa
    18181 tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt
    18241 tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat
    18301 acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt
    18361 atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg
    18421 aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt
    18481 ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta
    18541 tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa
    18601 gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg
    18661 atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa
    18721 ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt
    18781 cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt
    18841 tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat
    18901 atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga
    18961 gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa
    19021 agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag
    19081 ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa
    19141 acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa
    19201 ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt
    19261 ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact
    19321 tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt
    19381 cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact
    19441 gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc
    19501 tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa
    19561 atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc
    19621 tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg
    19681 actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga
    19741 tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg
    19801 ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt
    19861 taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat
    19921 ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg
    19981 ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac
    20041 cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga
    20101 aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt
    20161 tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc
    20221 atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt
    20281 tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt
    20341 tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat
    20401 gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca
    20461 tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat
    20521 gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga
    20581 gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg
    20641 gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta
    20701 aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca
    20761 ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag
    20821 aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat
    20881 cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag
    20941 tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg
    21001 atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga
    21061 atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa
    21121 ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata
    21181 atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg
    21241 aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat
    21301 aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg
    21361 aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac
    21421 aaacacaaaa cgcatctact gttatctacc agtagatgcg tttaaagaga agaacgagaa
    21481 tcgtccatat gagggtttcc
//
LOCUS       DAFEHS010000045        21453 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFEHS010000045
VERSION     DAFEHS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.046"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            226..894
                     /locus_tag="LP327_001749"
     CDS             226..894
                     /locus_tag="LP327_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBL1725864.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="LP327_001750"
     CDS             complement(1094..1480)
                     /locus_tag="LP327_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725865.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="LP327_001751"
     CDS             complement(1452..2834)
                     /locus_tag="LP327_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL1725866.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="LP327_001752"
     CDS             complement(2990..4177)
                     /locus_tag="LP327_001752"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBL1725867.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="LP327_001753"
     CDS             complement(4184..5215)
                     /locus_tag="LP327_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL1725868.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="LP327_001754"
     CDS             complement(5231..6685)
                     /locus_tag="LP327_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL1725869.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="LP327_001755"
     CDS             6860..7765
                     /locus_tag="LP327_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL1725870.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPCHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="LP327_001756"
     CDS             7856..8533
                     /locus_tag="LP327_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL1725871.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="LP327_001757"
     CDS             8530..8700
                     /locus_tag="LP327_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725872.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="LP327_001758"
     CDS             8814..9710
                     /locus_tag="LP327_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL1725873.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="LP327_001759"
     CDS             complement(9765..11135)
                     /locus_tag="LP327_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1725874.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="LP327_001760"
     CDS             11373..12119
                     /locus_tag="LP327_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL1725875.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="LP327_001761"
     CDS             12116..12955
                     /locus_tag="LP327_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL1725876.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="LP327_001762"
     CDS             12956..13906
                     /locus_tag="LP327_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL1725877.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="LP327_001763"
     CDS             14047..14799
                     /locus_tag="LP327_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725878.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="LP327_001764"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="LP327_001764"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBL1725879.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            16353..16961
                     /locus_tag="LP327_001765"
     CDS             16353..16961
                     /locus_tag="LP327_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL1725880.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            17129..18766
                     /locus_tag="LP327_001766"
     CDS             17129..18766
                     /locus_tag="LP327_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBL1725881.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(18838..19950)
                     /locus_tag="LP327_001767"
     CDS             complement(18838..19950)
                     /locus_tag="LP327_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBL1725882.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(20025..21398)
                     /locus_tag="LP327_001768"
     CDS             complement(20025..21398)
                     /locus_tag="LP327_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL1725883.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
BASE COUNT         6996 a         4064 c         4296 g         6097 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt gtcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg aggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 caccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctaaaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaaaa acatagaaaa acgattactt tgcttataca acaaggcttt tatggttttg
    16201 tatcaaaaaa atatttgttt aaatgttaca aatattaaat aattcgttat tttgtcatta
    16261 aatgtcatgt ttcttttacc tatgaaatat ttctaaatga taaagttaat agcgtcgaaa
    16321 gaataaaaaa cggagaaaaa aaggagttta atatgaattc atttaagaaa attgttttag
    16381 gtacaacttt tgctgctggt gctactgcaa tgtttgtagg aactacaaat gctcacgctg
    16441 atgaagttta tacagtaaaa tcaggtgatt cattatctaa gatctcacaa aaatttgcag
    16501 gagacaactc aatgatcgat gcaattgctg aaaaaaattc gatcgcaaac atcaaccgca
    16561 tttatgttgg tgaacaatta acaatcccaa cttcaaatga ttcttcagca acaacagaaa
    16621 acaaaacaac agaaaataca gcatctacaa ctgaaacagc aacgcaagaa catacatacg
    16681 ttgcacctgt tgaaacagtt gaagtagctc ctgctgcccc agctgctgca acagcgccaa
    16741 cttcaagctc tgcaaaagaa tggatcgcac aaaaagaatc aggcggttca tacacagcta
    16801 ctaacggacg ttacatcggc cgttatcaat tagatgcttc atacttaaac ggcgactatt
    16861 cagctgccaa tcaagaacgt gtagcagaac aatatgtaac atctcgttat ggttcatggg
    16921 atgctgctaa aacattctgg ttagcaaacg gctggtacta agccgataaa aggaaaatga
    16981 tgaaaccgag aaacttgttt attcaagttc tcggtttttt tgtgtgaaaa ataactcgtt
    17041 tgcttcccac agcgcagcag tacaaataaa aactcgaata atgtataata aagaaaacgt
    17101 ttttttgttt agggaatgag gaggagccat gagtacaaaa gagagtctcg aattaaacga
    17161 aatcacaagt ggaaatccac tgttatccag tattcgatcg attgtagaaa cgacatttta
    17221 cggtaataat gttcatgaag tttttgatag gaagacagct tatcaactag caaaagggag
    17281 tccaggaacg atcatcacag atttaacaat ctcacatgct gaagaactag atttaccagc
    17341 tgatgttagg acactagtat tcaatgatgg aagcattgtg ggaagaacag caagtgctcg
    17401 gcggatcttt gaggacttag acaaggaaca aagcaaatac gagaaaattt taagagaagc
    17461 tgtgtatcaa agtcgcaagc gccagtttta ccatacaaaa gtaatcgttg gcctatctga
    17521 agaattttct gtgcaaagtc atttgctgat tccagaagga tttgagaaca atttgtattc
    17581 ttatttgctg aattttcagc cagcgaattc gaagatctta gagacttatc aaaagtctgt
    17641 ttcctttgat gagggagatc tttatatctt ttgtgatccc gaatggacac atgaaacatt
    17701 tccaaatgga ctcattttgt ttgatgctct ccataataca gcggctgtgc taggattacg
    17761 ttactttgga gaattaaaaa aagcgacttt gacgcttgct tgggcattgg gaaatcgcaa
    17821 tggctttatt gcctgccacg gtgggatgaa acaatatgct agtaatgacc agacctatac
    17881 gatggctgta tttgggttgt ccggttctgg aaaatcaacg attaccttag caaaacatac
    17941 ggaaggtcac tctgtttcga tcttgcatga cgatgctttc gttatttctc gaaaaaatgg
    18001 ttttgctact gccttggaac cttcttactt tgataaaaca caagactatc cgtctgatga
    18061 tccggccatt tcttctttcc ttacttgtca gaatgtgggc gtgacatgta caagtaaagg
    18121 agaaaaagtc ctagtaacag gagatatacg aaacggaaat gggcggacag tcaaatcacg
    18181 ttatgcgaca cccgaccgag tcgatcatct tagcgaaaag atcgatgctg ttttttggat
    18241 catgaaagat gattcgcttc cgccaattat caagatcaat gacccagttt tagcagcgtc
    18301 ttttggtgca acattagcaa caaaacgatc agacgcggaa aatatcacta caaaaagaca
    18361 gctagatgag ctagtcattg aaccatttgc taatcctttc cgtgtctatc ctcttaggga
    18421 agactatcaa gcttttcgag agttgttttt gaaacagcaa acagcctgct atgtcctgaa
    18481 tactggatat ttcgatgata aaaaaatcaa acctgaaacg acattgtcaa tcgttgaaag
    18541 aatcgtgcaa caaacggcag aattcaagtc gtttggtcca tttgaagaca tccactattt
    18601 acctatacca gattttcctg tttcttttga ggatacgtcc tataaaaatc tggtgaaaca
    18661 acgacttgat attcgtttac gattcattga agctcagata ggaaaagatc tcttgccaga
    18721 tgaagcaaaa gaaagtttgg agaatttgat tcagaaattg aactaaaata acagcttttt
    18781 caagaagtaa tccatgcgca atatcgctcg ggatccttac ttgaaaaagc tgtttttcta
    18841 tgatggattt ttcgtaatat aggtggagat gtccttttga taatcttccg taggccaatg
    18901 accagtattt aagaagttgg ctaatagata gccgatataa ggacctgtgg tcaagccaga
    18961 agagcctaga ccgctagcag ccagtaagct tggatcatct ggcagtacgc cgaaaaatgg
    19021 ggaaaaatct gacgtataag cacgggtacc tacacggaac tcgtatggcc agttttttat
    19081 ctgatctgga tcagtcaaga actgttctgt actcttagtc aatcccttat aagcttcgct
    19141 agtaggagcc aaatcccagc cttgatcgtt ttcatgggta gcacctaaaa ggatctttcc
    19201 atccatgaaa ggaatgaaat cagcttctcc gtcaagcata gcaacaggcc agtcttgact
    19261 catcttataa gatgtctgaa atgctaagag ctgccctttt tgcggacgta tatctgcagc
    19321 atagcctaat tcttgtaata aaggcttaag tgccgggcca ggtgtcaaaa ttacacgatc
    19381 agctgtgtat tctccaaacg gtcccttgat gagccaagaa tcgctcttct tatgcaaagt
    19441 gacttttgcc tggatctgct ccatttgttt ttgtgctaaa cgctgaccaa gatgttctaa
    19501 atatgctttt ccatccagac gaccaccacc actgatacgc agtgaaactg ctggtttcaa
    19561 taacggaagt gcttgagatg tctgttcagg tgaaagccat tcgatgtttc caatttctgg
    19621 cgcttcttct tttcgtttct cggcaagaac agccaattca gctaaatctt tttcaggtcg
    19681 aagcaaaagt gtccctgatt gtgcgtaaat cgtttgatca agttggaaat cttttataag
    19741 tttagggaaa aaagcagcgc cgtctttagc cagacgatac cattgtttgt ttctgcgttt
    19801 cgacaaccaa ggggagataa tcccagcgct agctttagtt gcctgtcctg tcccttcatc
    19861 aaataaagca acattgaatt ttgatgtatc taagtaactg gctagggtca taccaatgat
    19921 cccgccaccg ataatggcaa ttttttccat aaaaaagcct ccttttacaa acaaccattt
    19981 tcacacaaaa aaacggcaga tgcaacacct gccgtatatg gaatctacgc ttttttagca
    20041 aagaacagat aataggcgaa tcgagcaaag cttacgagaa gcaacagcaa caagacgcct
    20101 aatgctgctt ttgatcctaa tgtagtagac acttcatagt tatgggaagt atgatgctgg
    20161 acgtaattga taatgaccgc tactaatgaa gtagccacag cgccagaaaa ctgctgcaag
    20221 gtattgaaca ttgcgttccc gtctccttgt agttcttcat tcagtgcatt cattccggtg
    20281 gtcatcatat tgctgtaaga taatccgata ccgatcatat aaaacacatg acaagcaatc
    20341 aatacaccca ctgaagcgtg tcctatgagt aggaataatg ctagccaacc aatcaaagaa
    20401 agaatcagcc cagcaaaaat tggcttcttc ggaccatatt gatccagcat tttcccggaa
    20461 aaaggagaaa gcaaagcacc aaccgctgcg ccgggaagca tcgctaatcc agcgacaaaa
    20521 ggactacttc cttgaacgat ctgaaggaaa ttcggcaaga caaaagatac accaagtaat
    20581 aaaaactgat aaactaagaa gccaatcaaa aacaagcgga atgttttatt tgaaagaata
    20641 gacagtcgga tcagtggatg ctccactttt ttacttcgat agtaaaaaag gaaccagcca
    20701 ataccgccga tgattaaagc aatccagctg gaaactgttc ccaacgtact caaaaatgtt
    20761 agacttccac taaacaaaag acaaattcct aaaacgctta tccaatccaa ataaccactt
    20821 ttttgtactg ccatctcggg aatagcaaac cagccgataa tcaaagaaag aatcaaaacg
    20881 ggcacaagaa acaggaagat atgattccat gtaaaatgtg aagtcataat gcctccataa
    20941 gtggggccaa ttgctggagc aatggccgtt gtcaaagtac cgacacccat catcgcgcct
    21001 cgtcgattga ggggggcaaa tgtcaagata atatggaaca tcaacggaag agcgatacct
    21061 gtcgctgcac cttggagcaa tcgaccaaac aataggacca tgaaggtagg agagaagaaa
    21121 tcgatcatta tacctgccaa gaaaagcaga tttgaagcta aaaagagttt tttgagacta
    21181 aaattgcgat tcaaataagt agacagcgga acaatgatcg aaatcatcaa aagataaatc
    21241 gttgttaccc attggacttg tgcagtcgtt aaatcaaact gtctgataag agttgggaaa
    21301 gttacattca tggccgtctc gatcaatacg cccgcaaaag acatcatccc tgttgcaatt
    21361 actgcaggta ctaaaagcag ctgtttttgt tttgtcattt acatcacctc taaaaaattt
    21421 cgtaaaaaaa gagagtgatt tctattataa aaa
//
LOCUS       DAFEHS010000046        21382 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFEHS010000046
VERSION     DAFEHS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.047"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(101..1597)
                     /gene="lysS"
                     /locus_tag="LP327_001769"
     CDS             complement(101..1597)
                     /gene="lysS"
                     /locus_tag="LP327_001769"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBL1725884.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1708..2712)
                     /gene="dusB"
                     /locus_tag="LP327_001770"
     CDS             complement(1708..2712)
                     /gene="dusB"
                     /locus_tag="LP327_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBL1725885.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2713..3600)
                     /gene="hslO"
                     /locus_tag="LP327_001771"
     CDS             complement(2713..3600)
                     /gene="hslO"
                     /locus_tag="LP327_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBL1725886.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3817..5928)
                     /gene="ftsH"
                     /locus_tag="LP327_001772"
     CDS             complement(3817..5928)
                     /gene="ftsH"
                     /locus_tag="LP327_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBL1725887.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6018..6563)
                     /gene="hpt"
                     /locus_tag="LP327_001773"
     CDS             complement(6018..6563)
                     /gene="hpt"
                     /locus_tag="LP327_001773"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBL1725888.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6563..8008)
                     /gene="tilS"
                     /locus_tag="LP327_001774"
     CDS             complement(6563..8008)
                     /gene="tilS"
                     /locus_tag="LP327_001774"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBL1725889.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8128..8598)
                     /locus_tag="LP327_001775"
     CDS             complement(8128..8598)
                     /locus_tag="LP327_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBL1725890.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8644..9069)
                     /locus_tag="LP327_001776"
     CDS             complement(8644..9069)
                     /locus_tag="LP327_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBL1725891.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9136..9405)
                     /locus_tag="LP327_001777"
     CDS             complement(9136..9405)
                     /locus_tag="LP327_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBL1725892.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9416..11011)
                     /locus_tag="LP327_001778"
     CDS             complement(9416..11011)
                     /locus_tag="LP327_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL1725893.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11026..14547)
                     /gene="mfd"
                     /locus_tag="LP327_001779"
     CDS             complement(11026..14547)
                     /gene="mfd"
                     /locus_tag="LP327_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBL1725894.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14564..15124)
                     /locus_tag="LP327_001780"
     CDS             complement(14564..15124)
                     /locus_tag="LP327_001780"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBL1725895.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15477..16451
                     /locus_tag="LP327_001781"
     CDS             15477..16451
                     /locus_tag="LP327_001781"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL1725896.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16621..18021
                     /locus_tag="LP327_001782"
     CDS             16621..18021
                     /locus_tag="LP327_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBL1725897.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18099..18821)
                     /locus_tag="LP327_001783"
     CDS             complement(18099..18821)
                     /locus_tag="LP327_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1725898.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18900..19406)
                     /locus_tag="LP327_001784"
     CDS             complement(18900..19406)
                     /locus_tag="LP327_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL1725899.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19529..21328
                     /locus_tag="LP327_001785"
     CDS             19529..21328
                     /locus_tag="LP327_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBL1725900.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5946 a         4687 c         3857 g         6892 t
ORIGIN      
        1 tgagccagaa tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta
       61 ttgacaaaga gccggaattc cttagacagc ttttattcga ttatctcatt gttgggaata
      121 gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg tcgatcccga
      181 tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag tcttcgtcta
      241 caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg aatctttctc
      301 tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc cccactatga
      361 atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct aaaggcgaaa
      421 cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca acaaatgttt
      481 caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct acattgtgtt
      541 tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct acaccagttt
      601 gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag tcgacagctt
      661 gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta cggataatac
      721 cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct tctaacatcg
      781 taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg ccgatttcat
      841 aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc gcaatacgca
      901 aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg gcagctccac
      961 ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca tctaaatagc
     1021 gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt tcgcgattac
     1081 tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag ccatggtatt
     1141 tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct ttgatagaaa
     1201 cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct ccaaggtctg
     1261 catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca taaatttgaa
     1321 tttggccttc acgatcttgt agatgcgcaa atccagtttt cccttttcct cggcgagtca
     1381 tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct tttgttcttg
     1441 gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt tttccaaatg
     1501 gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc atttgatcat
     1561 ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca ttctcatgtt
     1621 aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt ggaatttcgg
     1681 cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta ctttttccgc
     1741 tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg tctgattgat
     1801 tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg catgtgttct
     1861 gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta aatgaagttt
     1921 agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttctcctg tttcgagata
     1981 atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa tcatcacacc
     2041 gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga catccccgtt
     2101 ccccataaat gggatcgtca aatgtttttt cacttctttc aatacttccc agtccgcttt
     2161 tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg ctccagctga
     2221 ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac caatacgcat
     2281 cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt cgtatacttt
     2341 ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat ttactggaca
     2401 gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt gagcagcttc
     2461 aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt ctgtttcttc
     2521 aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt cactgatcat
     2581 ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg ctgaatttgt
     2641 tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc caattttcca
     2701 tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta attcttcttc
     2761 gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt gatcttcgtc
     2821 tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa atttttcttt
     2881 tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt cttctgtacc
     2941 aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg tagagattct
     3001 tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg cacctggcat
     3061 gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga ccaatacact
     3121 cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa aatcttcagc
     3181 gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc ctaagtcttt
     3241 tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt taccttcact
     3301 atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc cctttccatt
     3361 tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag ttagtttgtc
     3421 ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc ccaaagcagc
     3481 tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat ttgttgcttg
     3541 aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat aatctttcat
     3601 ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag caactactgc
     3661 ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag aaaacaggac
     3721 atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt gttccggttt
     3781 tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat cgtctgaatg
     3841 attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct cattcagttc
     3901 atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt ctttttcttc
     3961 caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat aatcatttga
     4021 ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt tggcatccaa
     4081 tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat gagcttcgat
     4141 gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt gatcaatctc
     4201 gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa caaacacttg
     4261 atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt ctgttaccat
     4321 gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg attggacatt
     4381 gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt caaacatatc
     4441 ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc cgccggcgcg
     4501 tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata ctaatccgac
     4561 aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc tgattacgcg
     4621 atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat cagaagcatc
     4681 gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga cattttctaa
     4741 atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa cgtcatctgc
     4801 catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta catctggacg
     4861 tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt ctagtacatc
     4921 agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc catccatttc
     4981 tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca taccggcacc
     5041 acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg cgtttttctt
     5101 cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca tttctacgaa
     5161 atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag cttttgctaa
     5221 taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga tacgggcacc
     5281 taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta gttcttgttt
     5341 ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt tgtctgcttc
     5401 tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc cgccgccttg
     5461 ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga agctaagcaa
     5521 tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa ctttgttttc
     5581 ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga ttgttgtgaa
     5641 atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt tagcgacggt
     5701 ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct gcacttcaaa
     5761 ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga tatctgacga
     5821 tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta agattacgag
     5881 gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag ttgccctccc
     5941 taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt gtaccataat
     6001 ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac accgatgtac
     6061 ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa ttcatttggt
     6121 acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg tttgtcaagt
     6181 agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac taaataagca
     6241 agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc ttctacattc
     6301 gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt cccgtagctt
     6361 gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat atctgccatg
     6421 aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg gtaaatttct
     6481 gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga gatcaaaatt
     6541 ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt tgatatttgt
     6601 aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat gaaaaggcaa
     6661 aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca tgctgccttt
     6721 gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg agattctctg
     6781 ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca tcagcaggaa
     6841 tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc cctgcatttt
     6901 caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt tctggaatca
     6961 gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta gagagtacaa
     7021 caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc cattggcttt
     7081 ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa ataattcctg
     7141 tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca gcatctgtca
     7201 tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg acttcctttt
     7261 cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc tgttgcgaaa
     7321 attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga actaccaaat
     7381 gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca aagtaaacaa
     7441 gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc aacggacgaa
     7501 tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta tttaactgtc
     7561 ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga tgagcagtca
     7621 tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa cgaaagttac
     7681 gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca tataaaggga
     7741 gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat tcttctctta
     7801 attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc acttgtaaaa
     7861 gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa attctcgatc
     7921 cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt aatagcggtg
     7981 cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct aaaaaaaaga
     8041 gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta tgacccaaat
     8101 agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc caccgcgctt
     8161 tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca aaaatgaact
     8221 catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa aagatttttt
     8281 ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt taggacggct
     8341 ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc cgatttttcc
     8401 atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta aaacatcatg
     8461 gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg tttttccagc
     8521 acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt gcagttttgc
     8581 tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt tttatattcc
     8641 tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg agctgtctgt
     8701 atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga atttgcttct
     8761 ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt gcagctgatc
     8821 aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt cgttcaattg
     8881 atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa tgaccaaagc
     8941 accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt gttgttgctg
     9001 aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt tattgctttt
     9061 tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata ccaatatcgt
     9121 gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa taatttcgta
     9181 cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt tcacttccaa
     9241 cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac tggaagattt
     9301 cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg ctactgatcg
     9361 tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt attcctcact
     9421 ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt agccattctc
     9481 gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc ccgatcccaa
     9541 ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca aatactatat
     9601 tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg aacatcaact
     9661 tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca attcgaacaa
     9721 aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct gctgttccta
     9781 ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat cctgcgcctt
     9841 ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct tggatgactg
     9901 cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca taatctttaa
     9961 acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca gctaatgcta
    10021 aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga tagatccggt
    10081 tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg gcaatcacta
    10141 aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc ttcgcaccgt
    10201 attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg atcagctgga
    10261 aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga aaacgccgga
    10321 tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac gctcctccat
    10381 gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca aaaacagctc
    10441 cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta aaagcaaacg
    10501 cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa acagcagttg
    10561 gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca ggcatcagca
    10621 aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc atcatccaag
    10681 caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc caaaatacta
    10741 agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt tctgcaacgt
    10801 actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc ccataaattg
    10861 gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg aggggaattc
    10921 ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca aatgctcctt
    10981 gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg ctgacagata
    11041 tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata ctgcttcttc
    11101 catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc ctagactggc
    11161 ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc gttttgttcc
    11221 aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt ctaggagcgc
    11281 tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt cttctggata
    11341 ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt gcgaatcaag
    11401 ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt atgtttcagg
    11461 tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt gactattctt
    11521 tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga aaccgactgc
    11581 atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa tggaaagatc
    11641 ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg cctgaagacg
    11701 tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag catatgcgac
    11761 acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc ccatataatc
    11821 agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt caatgatggt
    11881 tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat tttctaattg
    11941 cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga gttcttggat
    12001 ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata cttgtccacc
    12061 tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt ccataacata
    12121 cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat cccgcacgcc
    12181 taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca aaacatccac
    12241 ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt gttcttcgtc
    12301 aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc ggtgggttcc
    12361 tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt gttgctttct
    12421 tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc tttccagcat
    12481 cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta cttgtttgct
    12541 ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat aaccaacatc
    12601 acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga tctccgctgc
    12661 acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt ctttttggta
    12721 ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat ataatttgat
    12781 tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt tcgtccattc
    12841 acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat atttttggat
    12901 cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca aaatagtcat
    12961 atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt tcccgatacc
    13021 atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt tcaatcgctc
    13081 tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga aaatttcttt
    13141 ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca aagctccttc
    13201 aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta ctaatatgtc
    13261 ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa taaagatcac
    13321 aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg gcatttgtcc
    13381 gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac gaagatttcc
    13441 catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct gcacgatttt
    13501 ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga ttttttgagt
    13561 ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc gctgataatc
    13621 atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag attcttggta
    13681 aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc agcttgaagt
    13741 cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg tcgcagcaag
    13801 ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct ctggtccgta
    13861 gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg agcgttgtgt
    13921 ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta cacgaacagg
    13981 ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga attctcccgg
    14041 ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag gaagattttc
    14101 cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag ttttcttatt
    14161 cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt ttttttcttg
    14221 aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa aggccatttc
    14281 agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa tcacatgttg
    14341 cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa tcatgttttt
    14401 atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg ctaatcctgt
    14461 gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta cttgcggatc
    14521 ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg aatcgattca
    14581 tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca gattgacaca
    14641 tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca tgctggacta
    14701 ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag acattcgtat
    14761 tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc ttttgacgga
    14821 gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat tcctctggat
    14881 aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc atgaatgttt
    14941 gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact tctgcttcga
    15001 acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg accataaagc
    15061 cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg acgatcattt
    15121 tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact gctaattagc
    15181 ggtccagtcc atcgagcttt tttctttttt atacttgtca atgcatagta taacacaaaa
    15241 caaggcgttt cggctaaaag agaatcgtta ataaatgata aagaaattaa aaatttcttc
    15301 aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg taagctttta
    15361 catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga catggggctt
    15421 gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga tactaaatga
    15481 ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac ggcgccgtag
    15541 gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta ggtattgtgg
    15601 atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct ttggcattta
    15661 cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct gacttagttg
    15721 ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta gtaaacaaaa
    15781 acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt aacggtatct
    15841 ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa ttctcaggtt
    15901 tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt ttccgtcaag
    15961 cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc ttaggtgaac
    16021 acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg caaatctatg
    16081 aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta ttctttggcg
    16141 tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat ggtatcgcag
    16201 tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt cttcctttat
    16261 cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca cctgctgtta
    16321 tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc gagcaagacc
    16381 gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt gctaaactag
    16441 acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc gtttttttat
    16501 tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt cgtcttttcc
    16561 tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg agttgtcgat
    16621 atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc agttcttggt
    16681 gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac aaagcatttt
    16741 ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga aaaagcaaat
    16801 gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga tggaaaagtc
    16861 tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc ggagctttct
    16921 ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc agcggcagaa
    16981 aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac cgttcagaca
    17041 ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga tgcaacgatc
    17101 gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac gatcgacgtc
    17161 gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac aattgaattg
    17221 actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa agaacaactc
    17281 gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg tgagactttc
    17341 cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa agtcgattta
    17401 gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa cacaagtacg
    17461 aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt tggcacttac
    17521 agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat tctggcaggc
    17581 caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca tccattgatc
    17641 gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta caaagacgga
    17701 aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac acctgctgga
    17761 gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa tgacgatgga
    17821 acaccttacg agagtccggt aaactactgg atgccgattg actggacagg tgttggtatc
    17881 cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg cggttcacac
    17941 ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt cgaaaaagga
    18001 acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc gtacgattgg
    18061 tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc agcagtaaag
    18121 acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa ttgctctttt
    18181 tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc ttgcgccaaa
    18241 gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc agtataaatc
    18301 tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac atcctcaaac
    18361 atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc tttgcggtca
    18421 aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt aaaagctact
    18481 cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa gattcctact
    18541 ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc tacggcactc
    18601 gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc ttttgctttg
    18661 tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc tttagctgcc
    18721 atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc ttgggcattt
    18781 tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc tcccttgttc
    18841 ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc acagccaatc
    18901 tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt cagagcaccg
    18961 atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat catctgatgg
    19021 aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc tgtcagccac
    19081 caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat aaaataggta
    19141 ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc cagatggtcg
    19201 atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc tttaaatggc
    19261 tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta agtttgagaa
    19321 gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat atttccgttt
    19381 ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta gacttttttt
    19441 gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta tacttaattt
    19501 tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga atctagattc
    19561 tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc aattggaaga
    19621 ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg acaatgcaga
    19681 gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta cccaatgcag
    19741 cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa aggtcttatc
    19801 aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat tgtcaaagaa
    19861 atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt cgtttgaaac
    19921 aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat cagaagctga
    19981 agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca gtcattatga
    20041 tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag tcgaattatc
    20101 tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca gagaaacact
    20161 tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg atactttgaa
    20221 tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag attttctaca
    20281 aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt gggatacaat
    20341 ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc ttttcggtaa
    20401 agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt tgaaagaaaa
    20461 aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca aattcagtcc
    20521 aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag cagaagatcg
    20581 tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag aatcaaggat
    20641 ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg aactaggaca
    20701 tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat atgccatgaa
    20761 tgtcgcagaa acagcaagta cattcgcaga actgatcgta gccgatgcta cattaaaagt
    20821 cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa atgctttagc
    20881 tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg aagcacggca
    20941 agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc aaaaagaagg
    21001 ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat tgcatttctt
    21061 tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct ttagtttggg
    21121 catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata tccatttact
    21181 acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga atgtcgatct
    21241 tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata tccaaacgtt
    21301 catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa aaagagttag
    21361 tccagaatgg gctaactctt tt
//
LOCUS       DAFEHS010000047        21279 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFEHS010000047
VERSION     DAFEHS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.048"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            131..505
                     /locus_tag="LP327_001786"
     CDS             131..505
                     /locus_tag="LP327_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL1725901.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LP327_001787"
     CDS             complement(619..1248)
                     /locus_tag="LP327_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL1725902.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LP327_001788"
     tRNA            complement(1382..1454)
                     /locus_tag="LP327_001788"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LP327_001789"
     tRNA            1668..1741
                     /locus_tag="LP327_001789"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LP327_001790"
     CDS             complement(1879..2043)
                     /locus_tag="LP327_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725903.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LP327_001791"
     CDS             2338..3666
                     /locus_tag="LP327_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBL1725904.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="LP327_001792"
     CDS             3868..4701
                     /locus_tag="LP327_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBL1725905.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="LP327_001793"
     CDS             4717..5454
                     /locus_tag="LP327_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBL1725906.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="LP327_001794"
     CDS             complement(5609..6577)
                     /locus_tag="LP327_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBL1725907.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="LP327_001795"
     CDS             6809..7642
                     /locus_tag="LP327_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBL1725908.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLSPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="LP327_001796"
     CDS             7676..8515
                     /locus_tag="LP327_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL1725909.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="LP327_001797"
     CDS             8541..9101
                     /locus_tag="LP327_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL1725910.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="LP327_001798"
     CDS             9300..11021
                     /locus_tag="LP327_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBL1725911.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="LP327_001799"
     CDS             11186..12328
                     /locus_tag="LP327_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL1725912.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="LP327_001800"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="LP327_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBL1725913.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="LP327_001801"
     CDS             14128..14775
                     /locus_tag="LP327_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL1725914.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="LP327_001802"
     CDS             15168..17813
                     /locus_tag="LP327_001802"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBL1725915.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="LP327_001803"
     CDS             18123..19436
                     /locus_tag="LP327_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBL1725916.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="LP327_001804"
     CDS             19426..20079
                     /locus_tag="LP327_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL1725917.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /gene="radC"
                     /locus_tag="LP327_001805"
     CDS             20134..20826
                     /gene="radC"
                     /locus_tag="LP327_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBL1725918.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="LP327_001806"
     CDS             20976..21176
                     /locus_tag="LP327_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL1725919.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         7182 a         3551 c         4446 g         6100 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcagtcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggt
//
LOCUS       DAFEHS010000048        20282 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFEHS010000048
VERSION     DAFEHS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.049"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            137..457
                     /locus_tag="LP327_001807"
     CDS             137..457
                     /locus_tag="LP327_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725920.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            909..1109
                     /locus_tag="LP327_001808"
     CDS             909..1109
                     /locus_tag="LP327_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL1725921.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1256..4045
                     /locus_tag="LP327_001809"
     CDS             1256..4045
                     /locus_tag="LP327_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL1725922.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4092..4619
                     /locus_tag="LP327_001810"
     CDS             4092..4619
                     /locus_tag="LP327_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBL1725923.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4640..5830
                     /locus_tag="LP327_001811"
     CDS             4640..5830
                     /locus_tag="LP327_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL1725924.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5870..7168
                     /gene="asnS"
                     /locus_tag="LP327_001812"
     CDS             5870..7168
                     /gene="asnS"
                     /locus_tag="LP327_001812"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBL1725925.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8221..8895
                     /locus_tag="LP327_001813"
     CDS             8221..8895
                     /locus_tag="LP327_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL1725926.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8892..9233
                     /locus_tag="LP327_001814"
     CDS             8892..9233
                     /locus_tag="LP327_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725927.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9263..9622
                     /locus_tag="LP327_001815"
     CDS             9263..9622
                     /locus_tag="LP327_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBL1725928.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10146..12227
                     /gene="cadA"
                     /locus_tag="LP327_001816"
     CDS             10146..12227
                     /gene="cadA"
                     /locus_tag="LP327_001816"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL1725929.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12538..14004
                     /locus_tag="LP327_001817"
     CDS             12538..14004
                     /locus_tag="LP327_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1725930.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14619..15158
                     /locus_tag="LP327_001818"
     CDS             14619..15158
                     /locus_tag="LP327_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBL1725931.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            15232..15672
                     /locus_tag="LP327_001819"
     CDS             15232..15672
                     /locus_tag="LP327_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBL1725932.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15685..15894
                     /gene="copZ"
                     /locus_tag="LP327_001820"
     CDS             15685..15894
                     /gene="copZ"
                     /locus_tag="LP327_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBL1725933.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15894..18080
                     /locus_tag="LP327_001821"
     CDS             15894..18080
                     /locus_tag="LP327_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBL1725934.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18100..20271
                     /gene="copB"
                     /locus_tag="LP327_001822"
     CDS             18100..20271
                     /gene="copB"
                     /locus_tag="LP327_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBL1725935.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6774 a         3527 c         4407 g         5574 t
ORIGIN      
        1 aagaggttgt gacgtggtca cgacctcttt ttgacgatta tgatacaaaa aacatcattt
       61 atgaacaaaa tgtgaataac gggcgttctt gagatatgat tttaattggg aaaaacttat
      121 cgataaggag gtctttatga aaaaaagact gttatggtgt ttgctgtttc tatccctaag
      181 tttaacaagt cagccagttt ttgctgaaga aacacagcgg tctcctatta caggcacacg
      241 tctgtacaaa aatgtttatg tacagacaac agatcttgat tctgcgattc ctgaaacaga
      301 agaacggtct ttgcagccaa gatttggtta cttttggtac tgtccgacct gtgattatga
      361 aagttgctgg catttcttac attctacagc tgcagctttt gcttttactc atactcagaa
      421 atatgatgga catcatcctg ttatttatga ggtatagata tgagcaaaaa agaaaaacta
      481 atctcgttga tcgtattggt ttgccttgtt cttctggtaa ctgtcatagg ctatcagaaa
      541 taccatgaga acatcagacc tactaaagaa tcttccccag cctcgaccgt tgaaacagaa
      601 ccgataagta gtcagaaaga ataatttttt tagtttaaag aagaacatca gagcaaaaaa
      661 acaccaactg aacaatatgt cgtttatttt aaaaaaagtt cggtttttca ctttatgtaa
      721 tcgctgagaa aacttttttt aataaaaaaa gtcgaaaaac tctagctttt ttgtaaacgg
      781 tatgatatac tataggagta gtttttgttc ggtaatctgt ggtagcttgt ttcaaataaa
      841 catcttccag atatacacca ttcaagtaat tgcaaaatat gtgttgatac acaaggagga
      901 tttttattat ggaaacaggt acagtaaaat ggtttaactc agaaaaagga tttggattca
      961 tcactgcaga aaacggaaac gatgtattcg ttcatttctc agctatccaa ggcgacggat
     1021 tcaaaacttt agaagaaggc caagcagtaa ctttcgatgt tgaagaaggc caacgcggac
     1081 ctcaagcaac aaacgttact aaagcttaat taacctatac taaaaaataa acgcccagac
     1141 atttgcgtgt ctgggcgttt ttgtctattt tctttaaaaa gcgatgagag tatttttctg
     1201 cactttgtag tataatttca aaagaacatt agaggcagga gaagaggaag atattttgaa
     1261 accaagaact tatgcagtcg ttgatatcga aacgactggg acgaatccaa aagaagatcg
     1321 gatcatccaa tttggctgcg tcatgattga atcaggtcgg atcacttcac gtttttcgat
     1381 cgatattcat ccagggcgga agatttcaaa acaaatccag catttaacag ggatcacgaa
     1441 tcaacgcgtc caaaaagcgc cttatttcga agatgtcgcc caaacgattt acaatctttt
     1501 ggcagacacc atatttgtcg cgcataatat ttattttgat tataattttt tgaaccatga
     1561 actcatgcga tgcggacttc ctagtttgaa gataccaggc atcgatacag tagaacttgc
     1621 acaaattttc ttgccgacgg aaccaagctt tcgcttagct gatttgtcag aaagccttgg
     1681 atttattcat gagaatcctc atcaggcaga tagtgatgcc gaagtgacgg gtcaacttct
     1741 tttgctgatc gaagaacgga tgagaaaatt gccaatcatc acgttagagc agattgcgcg
     1801 attaagtaga cacacaggta tggatacgag tcgtttcatt tatcatgtga ttgaagaaat
     1861 gaaagaaaaa ccagagccac ttgatccctc attagaaatc gtcgatgggc tagctttgca
     1921 gaaaaaagaa gtggaactat tcacatccgt ccattatggc gagcggacat atccaagaaa
     1981 aaaacaagca aaagaaaaaa tgtttggtaa aacactgatg tatcgaaaag aacagaacag
     2041 actgatgaat gcggtctacg accatttcac aaaagatgaa tcaaaagatt tgatgatcga
     2101 agcagctaca ggaatgggta aaacaatcgg ttatctgctg ccattgagtt atttggctac
     2161 acctgaaaaa cccgctgtca tcagtaccgt ttcgctagtt ttgcagcagc agatcataga
     2221 aaaagacatt ccattgctga atcaattgct agatcagccc atacaacctg taattatcaa
     2281 aagctatcgc cactatattg atttacagcg ttttaaaggc acacttgtgg aaccgccaga
     2341 acaaaaacaa tatgctttgt atcaaatggc ggtcttagtt tggctgacgc aaacgaagac
     2401 cggtgatttg gatgaattac atttaacaaa tttgaaccat tcattctttg cagatatcgc
     2461 gcacagaggt attggatttt tagcaagaaa tcagtcattt tacgaacaag actttgttcg
     2521 tttcttgcaa aagaaaatca ggcaaagtaa ttttttgatt gtcaatcatg cgtttttaat
     2581 acaagaaaca cagcgaaaag aaccattgct tccagctagt ccatttttgt tgatcgatga
     2641 agtacatcat ttgccggaaa tcgctgaaaa agtaaatgac aaacgattaa gtattaacta
     2701 tttccataaa caaatccagc aattagaaga accaaatcag ctgtttgacc gaatacaaag
     2761 ctatttaccg aaagaacatg aagccttacg ctggttatcg atttatcgag aagaactttc
     2821 tgcgctatgc gaagcacaga tttgtatggc agaaggatta atgagtttag tgagcggcaa
     2881 gcaacctaac ggacagtatc cagaagaagt catgatgaca aaagagatgt tggatcagct
     2941 gccactggaa tctgggacag ccattgatca cctgcagctt ttttatcagg agatccagca
     3001 gctacagtca caaatgcgtc tgacttttga gattatccaa gaaagctgga caaaccagat
     3061 gcggatcgat ttagcaagtt tatatagctt atttgatcaa ttagacaagc agtacttgct
     3121 gatggatcaa tggctggaag actggcgaga tcatttgatc cattggttcg ttcctgggaa
     3181 cacagcgcag cagatgattt ttcatctcca tgattttcaa gcggctatgt tgccgtcgac
     3241 tgtttggtat cctcgatatg atcgtattct ttatattgga ggaacgctga agatcggccc
     3301 taatcgacac tatctctcta agcggcttgg cattgaagca gcacctctta aagtgattgc
     3361 ctctccttat gattatgaga aacaggcaag gatctatgtg ccaaaagagg cacctgccgt
     3421 ccatcaagtc agcaatcaag catatatcga ttatttagag acagcactta caggtctgat
     3481 cgaacaagaa gatcggccaa tacttgtgtt attcacttct catgatgttt tgcagaaggt
     3541 ctatcagaaa atgcatctgt ctatgctaga aaaaggacga gaaatactag ctcaagggtt
     3601 tggcggaagc cgggagaagc ttttgaaacg cttcattttg tcagaaaaaa gcgtattgtt
     3661 tggcgctgac agcttttggg aaggtgtaga tttgcctggg gatgctttgc agatcgttgt
     3721 agtcacacgt ttacccttcg ataatccgct tcgcccgatg gtcaaagcta ggaacgctta
     3781 tttagaatca gaggggatca atccttttta ccaggaatcc gtacctaaag cagctttgaa
     3841 gttacgccaa gcattaggtc gcttgataag gacggagaca gatcgtggcg tattgcttat
     3901 tttggatcga agactagtca ctgcaaaata tggtgcacgg atcatccatg cattaccgaa
     3961 aaaagtaccg atcaaagagc tagctatgga ggaaatctat caggacatcc atgaattctt
     4021 aaagaaaaat gaagaaggaa gttagaggag ctgtcttttt tcataattaa aatctgctat
     4081 aatgaaaagg aatgaagata aaggagcgac gacccttgaa gaaaaaacag acaaaggaaa
     4141 aaacgattaa tcgcgtgtta cttggcttga tcgtattttt attagcggtc atcgtgatca
     4201 gctcgatttt ttacttgcgt tccagtcgtc cggcggtgca ggcgaagaaa gaagcagtag
     4261 aaatcgcaaa gaaatatgca aatcttgagg aagccgatca tttttattgg tttactcgaa
     4321 agaagacata cttttcactg acaggtacaa atgataaagg aaaagaaatc gccgttatca
     4381 ttcctaaatc tggtgaaaaa gtaaaagtat tggatcaaac aaagggactg actgaaaacg
     4441 aagcaaaaca gacaattgct gcggcccatc ctgaaatcca aatcgaaaaa gctgctttag
     4501 ggatgtatga tgatcagcct gtgtgggaag tagtaggaaa agaaacaaat gggaaactga
     4561 attattacct gattggcttt gaatctggag atgaagtcaa gacaatcaag ggaatttaaa
     4621 aggtaaagga atgaatggga tggagttatc aaaaagaaca aaacgattag aaccgtctgt
     4681 cactttggca gctgcggcaa aggccaaggc attaaaagca aaaggcagag atgtgctgag
     4741 tttgactgtg ggagaaccgg attttgcgac accagaaaac atccaagaag cagcaatcga
     4801 agcaatccgc aacgggaaag ctagctatta tacaccaaca gcaggaatcc cagagcttcg
     4861 acaggcaatc gttgattatc tgaagaaaaa ctatggactt gtctatgaac cttcacaaac
     4921 agtcgtaaca gacggagcga aatttgcttt gtataccctt ttccaaacga tccttgaccc
     4981 gcaagatgaa gtcatcattc ctgtgcctta ctgggtcagt tacggtgagc aagtaaaact
     5041 ggcagaagga cttcctgtct ttgtgaaagg ggaagaatcc aatagctgga aagttactgt
     5101 tgaacagcta gaacaagcac gttcagaaaa aacaaaagcc gtaatcatca attctccatc
     5161 gaatccgact ggaatgattt atagcaagaa tgaactgcag gcaatcggtg aatgggcagt
     5221 aaaaaatgat attttgatag ttgctgatga tatttatggt cgtttagttt ataatggcaa
     5281 tgagtttact ccaattgcaa cgatttcaga agcaatcaaa aatcagacta tcatcatcaa
     5341 cggcgtgtct aaaacatatg cgatgacagg ctggcgcatt ggttatgcgg tgggaaatcc
     5401 tgtgatcatc aatggaatga tcgctgttgc ttcccagtca accagtaatc cgacagcagt
     5461 cagtcaatat gcagctgttg aagcattgaa aggcgagcaa gatacagtgg aagaaatgcg
     5521 gaacgccttt gaagaacgcc tgaatacgtt atattcgctt gtggcagaac tgccgggtgt
     5581 ttctcttgaa aagccacaag gagcttttta tctttttcct aatgtaaaag aaacgctacg
     5641 catgtgtaaa tatgaaaatg tcacacaatg ggtagaagat cttttagaag aaacaggggt
     5701 agcgcttgtg actggcgaag gctttggggc accagaaaat gtccgcatga gttatgctac
     5761 tgatcgcgct acgttagaag aagcggtacg cagaatcaag caatttattg agagtaaaag
     5821 tcaaaattaa tcctaaagaa attcaaggaa tataaaagga gagtcatacg tggaacaaat
     5881 tcaaatcatc gattcgaaaa accatgtggg agaaacagta acaatcggcg cttgggtcgc
     5941 aaataagcga tcaagcggaa aaatcgcatt tttacaatta agagacggaa cagccttttt
     6001 ccaaggtgta gttgtaaaaa gcgaagtgtc ggaagaagtt ttccaaacag ctaaaaatct
     6061 acaccaagaa acatccgtgt tagtaactgg agaaatccgt gaagacagtc gttcaaaatt
     6121 tggttatgaa atcggaatta caaatgtgga agtggtaagc gagagccacg attatccgat
     6181 cacaccaaaa gaacacggaa cagacttctt gatggatcat cgtcatttat ggctgcgttc
     6241 ttcaagacaa catgcgatta tgcagatcag aaatgaaatc attcgtgcaa cgtatgaatt
     6301 ctttaatcaa catcattttg tcaaaattga tccgccgatc ttgactggtt ctgcaccaga
     6361 aggcactacg gatctattcg aaacaaatta ctttgatcaa aaagcttatt tatctcaaag
     6421 tggccaatta tacatggaag ctgctgctat ggctttcgga aaagtctttt cattcggtcc
     6481 gactttccgt gctgaaaaat caaaaacacg acgtcatttg atcgaattct ggatgatcga
     6541 accagaaatg gctttcactc atcaagaaga aagtttagaa gtacaggaac aatatgtagc
     6601 attcttagta caaaatgttt tagatcattg cgaatatgca ttagatgtac taggacgaga
     6661 caaagaagtc ttaaaaactt atacaaaatt gccattccct cgtattactt atgatgatgc
     6721 gatcgaatta ctgaagaaaa atggatttga tgacatcgaa tggggcgaag actttggttc
     6781 acctcatgaa acgttcattg cgaattcatt cgaacagcct gtctttatcc taaactatcc
     6841 aaaagccatc aagccattct acatgaaacc acatccaaca agagaagacg tggtgatctg
     6901 tgcagatatg atcgcaccag aaggttacgg tgaaattatt ggcggaagcg aacgggcaac
     6961 agactatgat ttcttgctag aacaaatccg taatcacggt ttggatgaaa aagaatattc
     7021 atggtatctt gatctacgta aatacggttc tgttccgcac tctggttttg gtctaggtct
     7081 tgaacgaaca gtcacttgga ttgctggtat cgaccatgta cgtgaagcta ttccattccc
     7141 acgtttgttg aaccgaattt atccataaga ggtgatgaaa aagctgttct gcatcaagta
     7201 attagaacag ccaagcaaaa acagctgttc atgtttttga attggcggga cttattaccg
     7261 cagatgtaag cttttgaata ccgtttagtg gagaggttgt gaaaaagctg tttagtttcg
     7321 agtattaaga aaaattttgt ggaaatggct ttccacattt ttgcataaat ttggcttaat
     7381 accgagaaac tggcttttga acaccgttta aggaagaggt tgtgcgcctg ccgttcagct
     7441 ccgagtcaca aggagtaatt tcaaaaaaca gtttttcatg tttgatgact attgtgactt
     7501 gtgaccgagg agttggccgg agagcaccgt ttattctaaa aaagaactgc gaactctcgc
     7561 agttcttttt ttaaaatacg ggataaagga agtttccagc agacaagaga agaaagcaaa
     7621 agaaattgct atttgattca caaggttcta taacgaaatc gcaatcaatc aaacatttac
     7681 aaagaaggta attcaaatgg aaatctgatt tgatgatgct tctgctgttg aaataaagca
     7741 gcatctagca cctgaaaaca aactaatagt aacattcgaa gatggagtag gtccgtattt
     7801 tcagcatgca ataatccata tgtaagttca attcaccctt gaaatcatcg gaccagataa
     7861 agttatgacc aatctatccc aagcaatcta gaagagatat tagtaaaggg acatttacta
     7921 gaagtcttag atgagcctat aaatatatgt tctgatggcg tcatcagtgg tctgattgac
     7981 ggaagcttag gtgtgatcga tctctctgga ccaaacaacg aaggaatcaa ggggaatccg
     8041 gcacgttaag aaaaaaatag cataaaaaac tagacacttg atgagcgttc attcaattgt
     8101 ttgaagggac taggctggtg tcatttttta tacatatatt ttctctttcg tcttgatagg
     8161 gcttatggga taatataagg aagaaaatca tcgttttatt caaaaaggag tttttgacaa
     8221 atgaaaaaac gtatattatt tatcggtgat agcattacag acgcaggaag agaccgagca
     8281 gaagcaactg atctaggtaa aggttatcca cttttgattg ccggaaaact aatctcccaa
     8341 tatccaacga tggaatttga atgcatgaac cgtgggatag gaggagacaa aattgcagat
     8401 ctgcaagtac gctggcaaga agattgttta gctttacaac ctgactatgt atcgattttg
     8461 atcggaatca atgatacctg gcataatact agcgatcagg ctatattcgg aactgagaaa
     8521 tctgctcagc aatttgaatc cgtttatcga aaattactaa atgaacttca aacagtggga
     8581 atctgcaaca tcatcttgat ggagccgttt gtacttccta taaccgacga ccgtcatggc
     8641 tggcgtaaag acttagaccc taaaatccaa attgttcgta aacttgctaa ggaatatggc
     8701 gcattgctgg ttcctttaga tggtatattc aatgctttag ggattgttta tggtttttcg
     8761 aaatatacaa gagaagatgg ggtccaccca acgattggcg gacatgagat cattgcacaa
     8821 agctggatag atgcaattgc cgcagtcgat ttttttagtg gagataaaaa tgagcgaaag
     8881 aaaagtgaga aatgatggaa atacggtggg cagtaaaaga agatctacct caaatcaatc
     8941 agctgattac tgatcatcca gaatcattcg taaaagcaaa aacggcagct gacctaaatg
     9001 ataagaataa actattgctc gttgctgtaa caaaagaaaa acggattatc ggatttgcgc
     9061 aagcggacgt gcaacagtca gaagaacgat tggatcagcg accatcagga aaactaatcc
     9121 aacacacatt agtaacaaaa gaaaatacgg aagaaatcca aaaaaaattt gcagaatatt
     9181 tgatactgga agctaaaaaa agaagaataa aatcattttt tgcccagttt taatcaaaaa
     9241 cagtaataga aggagaaata caatgagttg tttaggaaat gtcatctggt ttatattcgg
     9301 cggactaatc ggtggtctta gctggttagc agcggggata ctttggtgta tcacgatcat
     9361 aggtatccca atcggtatgc aatgttttaa gatcgcctcc ctttcttttg cgccttttgg
     9421 caaagaagta atttatcacc agtctggcat gtcattgatt gttaatatca tctggctgat
     9481 cgtttcaggc ctaccaattg ctctcgcaca tgtcgtcagc ggcatccttc tatgtatcac
     9541 gatcataggg ataccttttg ccaagcaatc tttcaaactt gctcgattgg cgttgatgcc
     9601 attcggtgca gaaattgtat aaaagagaat aataaaggtt agataaagca tagagtcagg
     9661 tgggatacat gtatttcacc tgactctatg cgttctcctg tataagttct tccatgtgac
     9721 ttttacgtct tttatttata tggggttact gtttatggaa agttgaaata attttcatta
     9781 aagaactctt tctatatttt tgaaagcgta gtggagcttc gaatatctgg gaaggacatt
     9841 ttgcagggat tgaaaaaaga tattgtccta tcgaaaaatt aatgaattta gacaaattgc
     9901 cagcaacagg cttcaaactt agttgctatc ctgtcaagat caagaaggca agtgcgggtt
     9961 ggataagagc agtggcaatg atcgaaaaga aaaagaaaga ataagccaaa acatctgtat
    10021 ggaagaaatt ttccgatgca gatgtttttc attttttctt tcggatacct tgacatatga
    10081 ataaccgttc atttataatt agttatgaag agatattcat atgttgaggc gaggaggtca
    10141 ttaaaatgaa aagttacaga ttagaaggtt tagattgtgc gaactgtgca atgaaaatcg
    10201 aaaaaggagt tcagaagatc aatggagtca aggaagcaac ggtaaacttc acttctggaa
    10261 aactaacgat tgacgcagag gaagatcatt tggctacgat tgaacaagaa ataaaaaaag
    10321 tagtgaagga attagaacca gatgtcaaag taacagaaat agataaggaa aaagtatctg
    10381 aacatggaaa tgaaaaagag agaaatacgc tgttccggat tttattttcc cttgcaggta
    10441 tcgctttatt gctattattt gattttaatg aaccaattcg cttgataggg tatttattga
    10501 tttatctgct gataggatat gatgtagtga agaaggcagt gatgaatatc gtcaaaggaa
    10561 aaatctttga tgagaatttt cttatgtctg ttgctacgat tggcgctatg ttgatcggtg
    10621 aatatccaga agccgtagca gtgatgctat tttatcaaat cggcgagtat ttccaaggat
    10681 tggcagtcag tcattcaaga aaatcgattc gcgaattgat ggcgatccgt ccagaaacag
    10741 ctcacgtgca gacgacggaa ggattgatga ccgtgaaccc tgaagacgtg cagatcggtc
    10801 agtttgtact tgtgaagcct ggtgaacgtg taccgttaga tggaacgatc atcgaaggag
    10861 aatcattagt cgatacgtca gcacttacag gagaatcagt ccctaaatct gtatatgttg
    10921 gtgaaacagt attaagtgga tttatcaata aaaataaacc atttttagtt caagtagaaa
    10981 aatcttatga aaattctact atcagcaaat tattagaatt agtagaaaat gccagcagta
    11041 aaaaagcacc agctgaaaat ttcattacta aattcgcgcg ctattacaca ccagtcgttg
    11101 taggattggc cgttttatta gctgttttac ctccacttgt tgtttctgga gcggcgtttt
    11161 ctgaatggat ctatcgagca ttgacatttt tagtgatctc atgtccatgt gcattagtga
    11221 tctccgtacc ccttagtttc tttggcggta tcggaggagc aagtaaaatc ggtgtattag
    11281 taaaaggttc aaattacttg gagttgcttg cacaaactga gacagtggta ttcgataaaa
    11341 ccggcacatt aacgaaaggt gacttttcga tccagacgat cgatacaaaa atcgatccaa
    11401 agatattcat gcaatatgtt gcgagtgcag aacagttttc tacacatccg attgctcaat
    11461 ctgttttaga aggctacgca ggtccgcttt taccaacagc taatatccaa gaatttgcag
    11521 gtgaagggat cttagctgaa gttgatggca aacaagtatt agcaggtaat cacaaattga
    11581 tggaaagatt tgaaatctct tttccttcaa gtcaagaaat cggtacattg ctttatctag
    11641 cgatcgatca aagttacagt ggttatcttg ttattgcaga taccttgaaa gaagatgccg
    11701 tagacgcatt ggttcaatta aaaaaggctg gtgtgaagaa cacagtcatg ttgacgggcg
    11761 attcgaaaaa aattgctgat catatcggga agcaagtagg cgtggacaag atatatagcg
    11821 aattgcttcc agaagataaa gtgcagcggt tagaggaaat cttacaaaga aacaataaaa
    11881 agaccgcatt tgtcggagat ggaatcaacg atgcaccggt gctagcaaga gcagatgtag
    11941 gaatcgcaat gggtggattg ggaagcgatg ctgcaattga agcagcagat gtagtcatca
    12001 tgaatgatca accatcgaag attgcagaag caatccattt agcaaagaaa acattgaaaa
    12061 tcgtcaaaca gaacattgtc tttgctattg ggataaaaat tttggtactt gccttaggtg
    12121 cttttggttt tgcatcaatg ggagatgcag tatttgcaga tgttggggtg acagtattag
    12181 ctgttttgaa tgccatgcga agcttgcatg taaaaaataa acgttgatca atgaagagga
    12241 cgaatgaacg tcctcttttt tttatatcct tttcttaatg aaaaatgtaa agaaaataga
    12301 taggattata aaataactaa tgtttttaat tttgcagaaa attcaaaata gcttgaaatt
    12361 caataaggta aatgaaataa aagaaaataa cttatttttt tgatggaaaa aatatgaaaa
    12421 aacgatgtgt attaaataaa agaatacaca cataaaataa cactgtcttg taagtttgtt
    12481 aaaaattgaa tatgattaca tctggataac aaatttttat cataaaggag agaacgaatg
    12541 aacaacaaac aattacgctg gtttactgtt gcgttaattg catttaacat ggtttggggc
    12601 atggggaatg tagttaataa ctatgctcag caaggagtat cagttgtcac ctcttggcta
    12661 ttgattttgg ccatttattt tattccatac gcattgatcg ttggacaatt agggtcagcc
    12721 ttcaaggata gtaaaggagg cgtcagttca tgggtcgaaa atacgacatc taataaacga
    12781 ctggcatact atgccgcttg gacttactgg gtcgtccata ttccttattt ggcacaaaag
    12841 cctcaagcaa tcctgattgc tggcggttgg gcagtccaag gaaatggaaa tatcgtaaat
    12901 acgatgtcag tacaaatggt agcagttatt tcacttatta tatttcttgc ctttttatat
    12961 ttatcaacaa aaggactttc tactttgaaa gtgattggcg gactggccgg aacagcgatg
    13021 ttcgtcatgt cgatcttatt tattctttta gctgtaacgg caccgttcat caatccaacg
    13081 atggaatttg ccacaccaca tatgaatcaa gtgaaaacat atattccaac atttgatttc
    13141 agttatttca caaccgtttc gatgctagta tttgcagttg gaggagcgga aaaaatatcc
    13201 ccttatgtca actctacaag aaatcctgca aaggaatttc ctaaagggat gatcttctta
    13261 gcagcaatgg tagggatttg tgctgtatta ggctcagtcg caatgggtat gctgtttgct
    13321 agcgataata ttcctaaaga cttgatggca aatggggcat acagtgcttt tcaaatttta
    13381 ggtaattact atggtatcgg taactcattg atgatcattt acgcattgac aaacatgatt
    13441 ggacaaatct ccgcattagc cttttcaatt gatgcaccac taaaaatctt gttagcagat
    13501 gcggatccag aatttgtacc aaactggcta cgtaaacgta atcgtaaagg tacattggtg
    13561 aatggttatc ttctgacagg tatactagta ggaatcatta ttttgttgcc gatctttggt
    13621 atcaaagata tgaatgaatt agtaaaatgg ttgacgaact tgaactcagt tgtcatgcca
    13681 atgcgttacc tatgggtgtt cttggcatat atgatgttga ataaagctta taagaaattc
    13741 aattctgaat ataaatttgt taaaaatcca aaaatcggtt ttatctttgg tgcttggtgc
    13801 tttgccttca ctgcgtttgc atgtatccta ggaatggtgc caaaagtaga ttatactgct
    13861 gatcctaaag catggtggtt ccaattattc acgaatatca tcacaccaat tgtcttgatc
    13921 ttattaggta tgatcttgcc agcaattgct cgtcgagaca aaaacaaaga aatcactgaa
    13981 ccagaaacag tggttcagcc ttaaaataga aatacttgtt acaagaggtt gtgacagaag
    14041 tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaag
    14101 tttcggcttg tttttcgcag aagctacttc tggaacaccg tgaatcagag gttgtgaaaa
    14161 agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagcccctca tatttttcgt
    14221 tttgctgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggtagtg
    14281 agcctgccgt ttaactccga gtcacaagga gcaatttcaa aaaacaactt ctcatgtttg
    14341 ttgactattg tgccttgtga ccgaggtgtt ggcaggagaa caccgtttaa ggaagaggtt
    14401 gtaaaatcag cgatttgacc tgagtattag aggaacaaac gaaaaaatag ctcctcatat
    14461 tttgccgttt tttgagttaa tgctgaaggt cagctgattg aacaccattt attcgaaaca
    14521 agggactttg acacgacttt tgtcacagtc ccttgttttt ttgcggcgtt ataagtggta
    14581 tgatcgaacc aaattccaaa gagaagtgag ggagaagaat gaagatcatc gtgattggac
    14641 aaagtgggtc gggaaaatcg accctcgcaa ggaagatcaa agaaatcacc gactttccgc
    14701 tactgccgct agacctttta tggcatacaa cagactacag tattcaggca aaaaagtggt
    14761 ttttgcaaga acagcaatcg tttatggtat ctaatccgtc atggattgtt gaaggaaatt
    14821 atacttctac attagaggaa aggattaaag aagcagataa gatcatttgg cttaaagtgc
    14881 cgcgttatct cgccatctac cgagtagttt ggcgttctct aaaaagaaag ataaacaaaa
    14941 aatctcggcc agatatgcct gaatcatttt cagagaaact aaacagggaa tacttagaat
    15001 ttttatcttt tatctggcat tttgaagaaa acaatgaacc gaaaatacag cagctgatcc
    15061 aagtagctaa tgcaagaaat aaactgatca tccttaaaac aagacgagag aaacaagaat
    15121 ttttaactaa attgaaaata gagagcaaaa ataattgaca gtttttgttt acaagcgtaa
    15181 tctgtattca taagctaatg attacatttg taaacgaaag gtgtgaaata aatggaaaaa
    15241 gtcaaaacgc cagtaaagat cagtgattca gagtgggaga tcatgcgtgt tatttggaca
    15301 cttggacaga cgacagccca agaaatcacg cagatcttag ctgattcgat ggattggaaa
    15361 ccagcaacga tcaaaacttt gctgggacgt ttggtaaaaa aagaagtgat ctggacagaa
    15421 caagaaggaa aaaaattcat ttatcatcca gctgtctcgg agatggaaac agttcgcagt
    15481 gcgacggaaa atcttttttc ccatatctgt gcaaaaagga ttggagagac gattgcggat
    15541 ctagtagaag aagccacttt gactcaagaa gatatcaatg agattttgaa acaactggaa
    15601 caaaagcaac cagtagatac gatcgaatgt aactgtatcc caggtcaatg tgaatgtaaa
    15661 caacatcaat aggaggaaga aaaaatgaaa caacaatttt caatcgaagg tatgagctgc
    15721 aatcattgtg cagcaagagt agaagaaaca gtaagcgcac ttgacggcgt gcaaaaagtg
    15781 aaagtaaatt tgaaaaaagc aaacggtacg gtcaagtttg atgaaacaaa agttcaatcc
    15841 gaaaagattt gtcaagcaat caatggattg ggttataaag cagaggtgat ttgatggcaa
    15901 cagcagaaac caaaatggat acgttcgtca ttacaggtat gacttgtgca aattgttctg
    15961 ctaggatcga aaaagagctg aacggacaac ctggtgtggt caatgccaca gtcaacttgg
    16021 ccactgaaaa agcaagtgtc aaatatgaag ggacgaccac tgaaaaatta atccaaagcg
    16081 tagaaaacat cggttatggt gcaattttgt acgatgaagc ccacaaacaa aaaattgcag
    16141 aagaaaagca agcgtatttg aaaaaaatgc tattcgatct tatattgagt acggttttga
    16201 ctttgccgtt gatgttgtca atgattgcta tgatgttagg aagccatgcg gcgattgttc
    16261 acttttttca ttttccaatc gtccagttag tgctgtctgc tcctgtccaa ttttatgtcg
    16321 gtgctcgttt ttataaaggt gcgtaccatg caatcaagac gaaagcacca aatatggatg
    16381 tattggtagc aattggaaca tcggctgcct ttgcattgag catttacaac ggatttttcc
    16441 gtggacaccc ccaagatctt tattttgaaa gcagcagtat gatcatcacg ctgattttgc
    16501 tcggcaaata tttggaacat acagcaaaaa cgaaaacagg taatgcaatc aaacaattga
    16561 tgtctcttca aacaaaaacc gcccaagtga taagaaacgg gaaagaagaa acgttagcga
    16621 ttgaagaagt agtcgttggt gatcaactag tcatccgtcc tggtgagcag attccagcag
    16681 atggtcgaat tatttcagga agcagtgcga tagatgaaag tatgcttact ggtgaaaatc
    16741 ttcctgtaga aaagaatccg gatgacacgc tttttggcgg gacaatcaat acgaatggtc
    16801 tgctacacat ggaagtcaca caagtcggca agcagacagt attagcgcaa atcattcaaa
    16861 tggtggaaga tgctcaaggg agcaaagccc caatccaaaa aatcgcagat cggatctcag
    16921 ggatttttgt tccgatcgta ttagtcatcg cgtttatcac gctcatagct acaggtttga
    16981 tcactggtga ttggcaactc gcattgatcc atagtgtctc tgtattagtc attgcgtgtc
    17041 catgtgcatt aggtttagcg accccaacag ctattatggt aggaacgggt gttggtgcac
    17101 gaaatggaat tttaattaaa ggcggagaag cattagaagc agctgctcac ttagacagca
    17161 ttgttttgga taaaactgga acgattacag aaggaaagcc caaagttaca gatctagtcg
    17221 gttcaaaaga agtcctatct attttttata ctttagaaca agcttctgaa catccactag
    17281 gtaaagcaat cgtggaatac ggaaaactgc aagaagcagc aacatacgat atgattgatt
    17341 ttacagccca cccaggtgct gggatcagcg gtacgattaa tggagtacgc tattttgcgg
    17401 gtacacgtaa acgattaatt gaattgaacc tatcatttga tgaataccaa gaacacgcgt
    17461 tagaattaga acagcaggga aaaacagtca tgtttttagc tgatgaaaag caagtgatcg
    17521 ggtggattgc cgtggctgat caaatcaaac tagaagccaa acaagcaatc aagcagctcc
    17581 agaataaagg attagatgtt tttatgctga caggagacaa caaactggca gccgaaacga
    17641 ttggtaaaca agttggtatt gatccaaagc atatctttgc tgaagtactt ccagaggata
    17701 aagcagccta cgtagaaaaa cttcagaaag atggcaaaaa agtcggtatg gcaggcgatg
    17761 gaatcaatga tgcaccggcc ttagcattag ctgatgtcgg tatggcaatg ggaagtggaa
    17821 cggacattgc gatggaaaca gccgatgtga cgctgatgaa tagcagtttg gcatccattg
    17881 cacaaacaat cgagttgtct aggttgacat tgagaaaaat caaacaaaat cttttctggg
    17941 cttttgtcta taatacgatt ggtattcctt ttgccgcact aggctttttg aatccaatca
    18001 ttgcaggagg agcgatggcg tttagttccg tcagtgtatt gttgaactcc ctgagcttaa
    18061 atcgacacat gaaaaagtaa gagaagggat ggagtaaaaa tgaatgggaa cgatatggag
    18121 aaaaaacatg agcataaaaa agaagtagta aacaaagaaa gtacagaatc tcatgaaaaa
    18181 cagatggaga tgaaacatga ccatagtcag atggatcatt ccatgcacat gggacataac
    18241 catggagata tagcaaacag caaacaaatg gaccattcga tgcacatgga tcacgaacac
    18301 ggcggcatgg atcattccat gcacatgggg aatttcaaac aaaaattttg gctatcgctt
    18361 atcctagcta tcccaatcat tcttttttca ccaatgatgg gaatggaatt tccatttcaa
    18421 gtaacgtttc ctggttctga ttggctcgta ttgatcttag caactatact atttatctat
    18481 ggcggtcagc catttttaag tggagctaag atggaattga aacaaaaaag tcctgctatg
    18541 atgacactga tcgcgatggg gattacagtt gcatatattt atagtgtcta ttcgtttatc
    18601 gcaaatctgc tcaatccaca tacacacgtc atggatttct tctgggaatt ggctactttg
    18661 atcgttatta tgctactagg tcactggatt gaaatgaatg ctgtgtcgaa tgccagcaat
    18721 gctttacaaa aactagcaga attactgcca gaatcagtga aacgcttgac gaaagatgga
    18781 aaagaagaga cagtttcatt gaaagaagta aacgaaggtg atcgtttgat cgtccggtct
    18841 ggtgataaaa tgccaacaga tggtgtaatc ttgaaaggtg aaacaattgt tgacgaatca
    18901 gctgtgactg gcgaatcaag aggaatcaaa aaacaagcca atgacaaagt gattggcggt
    18961 tctatcaatg gggatggaac gatcgaaatc gaagtaactg gaactggtga aaatggttat
    19021 ctagcgaagg ttatggaaat ggtgcgaaaa gcacaaggag aaaaatccaa gctagagtca
    19081 ttatctgaca aagtggcaaa atggctgttt tatgtcgcat taattgtggg tatcctggca
    19141 ttcatcgcct ggttgttctt gacagatctt cctaatgcat tagaacgtat ggtaacagtc
    19201 tttatcatcg cctgtcctca tgctctaggt ttagctatcc cattagttgt tgctcgctcc
    19261 acatcaatag cagctaaaaa cggattactt ttgaaaaacc gcaatgcact ggagcaagcc
    19321 aatgatctag atgtgatcat gctggacaaa acaggaacac taacagaagg aaaatttact
    19381 gtaacgggtg tcgaagtact tgatgatgcg ttcaataaaa atgaaatcct tcaatatcta
    19441 ggagccttgg aagcgaatgc caatcatcca ttggctgttg gcattatgaa ttatttgaaa
    19501 gaacatgaaa tcaagcctta tcaagctgaa aatctaaaaa atctttcagg agtcgggcta
    19561 gaggcaacag tcaaaaaaca acaagtgaaa atagttaatg aaaaagaagt tgagcgtctc
    19621 ggattaaacg tagaacaagc attattgaaa ccttaccaag aacaaggaaa taccatcagc
    19681 ttcctgattt tagaaaatca tctagctgca atcgttgctt taggtgacgt agtaaaaaca
    19741 gaagcaaaag aatttatccg aacattgaaa gaaagaaaaa tcactcctgt catgcttact
    19801 ggtgacaata aaaacgctgc ccaagcagta gcagattatt tagggatcga agaatattat
    19861 ggcggtttat taccagatga taaagaagct gttgtccaga aataccttga ccaagggaaa
    19921 aaagtaatca tggtgggtga tgggatcaat gatgcgccaa gtttagcccg tgcttcaatc
    19981 gggatggcaa tcggtgcagg gacagatatc gcaatcgatt ctgcggatgt cgtattgaca
    20041 aacagcgatc caaaagatat tttgcatttt cttgatctag ccaagcaaac gcgaaagaaa
    20101 atgatccaga acctctggtg gggggcaggt tacaatattc ttgcaattcc gctagcagcc
    20161 ggaattcttg cgccaattgg tatcatatta agtccagcag ttggtgctgt cttgatgtcg
    20221 ttaagtacgg tagttgtagc tctaaatgct ttgacattga aaatcaaata ataatgaaaa
    20281 aa
//
LOCUS       DAFEHS010000049        20218 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFEHS010000049
VERSION     DAFEHS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.050"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            215..1516
                     /locus_tag="LP327_001823"
                     /pseudo
     CDS             215..1516
                     /locus_tag="LP327_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1714..2310)
                     /locus_tag="LP327_001824"
     CDS             complement(1714..2310)
                     /locus_tag="LP327_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HBL1725936.1"
                     /translation="MKTVKKMIKLVFASLGIIVFLGACSNQSESNNSKSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            complement(2331..2537)
                     /locus_tag="LP327_001825"
     CDS             complement(2331..2537)
                     /locus_tag="LP327_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725937.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            complement(2552..3946)
                     /locus_tag="LP327_001826"
     CDS             complement(2552..3946)
                     /locus_tag="LP327_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL1725938.1"
                     /translation="MRKRTIKTQLVLSFLAIATLIIGSISLVALSLTNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKALENNIYFSIEDEQGNMVWELKGK
                     NLKSAQDKLKKNALKVSEKKSVKLDETIEAKKKLINDGNEFGKVTFYYFGPFAYTEHD
                     ALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIPQ
                     ETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGVW
                     KITPERLQSCYDEIDRLTRLIGNIEVINKIEAKYDHLNKTEFNIYKLIESVIENFASK
                     IESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVLL
                     IVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVVEAHKGSITVKSK
                     LGLGTKFVIKLPFQ"
     gene            complement(3946..4626)
                     /locus_tag="LP327_001827"
     CDS             complement(3946..4626)
                     /locus_tag="LP327_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1725939.1"
                     /translation="MKILIVDDEEKILEIIDAYLVANHYSVYKAISGAMALEKFEKNN
                     PDLVVLDLMLPDTDGLTVCRKIREVSNVPIIMLTAKSDEDDILTGLKLGADDYMIKPF
                     SPKELVARIQTVLRRTETLSNPNKLSSSNGELIVYPESRQVYLHQVELNLTTTEFDIL
                     HALISTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHIKNLRHKIENDAKSPMYILTVHG
                     TGYRFGGH"
     gene            complement(4654..6237)
                     /locus_tag="LP327_001828"
     CDS             complement(4654..6237)
                     /locus_tag="LP327_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HBL1725940.1"
                     /translation="MWGNQHKISTYFFYGNYIIDMGDDGLKNNKYIWLSATISMLTLG
                     GVFILIQQQNKEEHAEVVFQEEQRQISSRMSNMMGDNQEADLKVVHQTERQLLIPPVL
                     EPSSKSKENVIYDIVAQNGEVQIMDGEKTETLGYNGDFLGPVIRLKKGQKVTINTNNN
                     LDASTSFHWHGLKVASDADGGPHQIIETGQKKSVTFEVDQEASTLWFHPHPEGETASQ
                     VYKGLAGLMYIDDDNSKSLDLPSKYGVDDIPLIVQDKSFSSTNQINYENDFNSDGTKG
                     ETLLTNGTINPYIEIKNRWMRYRIVNGSNSRNFTFTLDKDESFYQIATDGGFLNTSVK
                     LSKLLLAPGERAEILVDTQNYKKGEVIHLLANNLVALTMRIENTIENKEFTPSDSLNT
                     ISTLDEKKLEDLARQSINLRGMSHMVNINNKQFDMERIDLYKKLGTQEIWEVNNISSM
                     MGGMIHPFHIHGVQFQILSRDGNQPALNEQGWKDTVLVNPDETVELLVKFDREGIFMY
                     HCHILEHEEYGMMGQMEIK"
     gene            complement(6248..6472)
                     /locus_tag="LP327_001829"
     CDS             complement(6248..6472)
                     /locus_tag="LP327_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBL1725941.1"
                     /translation="MMKYLQIVVIDGMNCSRCASKVRNALEAEPTVSVKIVDLARGIV
                     TFDADSKLTLTFLNQLLKNTKYTVTRNISI"
     gene            complement(7105..7323)
                     /locus_tag="LP327_001830"
     CDS             complement(7105..7323)
                     /locus_tag="LP327_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBL1725942.1"
                     /translation="MTEHLQPLFISGMACLGCAGEIKYALEKNPSISVRKVNLEEGLV
                     TIATKTDLSISVINQLLKNTNYKALQVW"
     gene            complement(7340..9790)
                     /locus_tag="LP327_001831"
     CDS             complement(7340..9790)
                     /locus_tag="LP327_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL1725943.1"
                     /translation="MISKYNLYGMTCAVCATTIEKKIHELDGVYFAKVNLTTEVLKLE
                     YDEGILSNHTVITAIQDIGYDAEIRKKTEIKVFGISGMTCVSCANKIENVVKSMTYVK
                     YANVNFEAEKLSVISTDPSAVAKISDVVKILGYQLYALSEDEEQVIYKTKEQEQQQQK
                     LRRRFITSMLFTIPIIYVASANILELPLFEIIDPTTNPSFFSMTQLILTAPVILANKD
                     YYKIGFSSLMKKHPKMDILIALGTSIALLYGIFATIQIILGNYLYTKELYFGASAVIL
                     TLIRLEKYLELVTKDKTSEAIQKLMRMTPKTAKTIRHNKEQTVPIESVLVGDVIIAKP
                     GERLPLDGIVISGKSLVDESMPTGENLPVEKKANDRVIGPSKNKNGVIRYETTHIGKD
                     TTLAQISKLVDDAQSSKAPIEKLADIISSYFVYIIIVVAIGSSLLWVLSGQSISFSFS
                     VLISVLVITCPCAFGLATRTAIMVGMGKGAENGILIKSGEALETTHHVKTILFDKTGT
                     ITKGKPVVTDIILAENMDRNFLLFLAASAEKASEHPLGEAIVKEAEIVGIDLVEPSIF
                     ESFSGLGVNAILKGTEVSLGNEEFFKQLNIDISKFKLISDNLVSKGKTPVYIANDRIF
                     LGIIAIEDTIKPASALAIQKLNKLGLNVVMLTGDNKLTAQAIANQVGITNVISEVLPQ
                     EKAYYVKMLQSKEHRVAMVGNGINDALALAQADIGFVMGSGTDIAMESADIILMNDDL
                     QLLMSTIDLSKKTIWNIKSNLYWAFAYNMLSVPIAMGVLNIFWRSLLSPLIAGVAMSF
                     SSITVLLNSLRLKNFKVK"
     gene            complement(9774..10139)
                     /locus_tag="LP327_001832"
     CDS             complement(9774..10139)
                     /locus_tag="LP327_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBL1725944.1"
                     /translation="MVLVEHKIQVSNSELDVLKFIWRYEPVTCGLITHGMQERNSWHP
                     STTKTLIRRLLDKNVITFNTSKNQRYYTSLIKKQEFLETEIQRLLSGMDEGCISEVSY
                     YLNGLVKRSDTEEFNDFKI"
     gene            complement(10155..10769)
                     /locus_tag="LP327_001833"
     CDS             complement(10155..10769)
                     /locus_tag="LP327_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL1725945.1"
                     /translation="MKKSMLFLLFFVFALSLAITLTINFPGLLKINLFFQSDINFQNW
                     SRSQVNQDFRNLMGYLNNPFQKELVFDNLYVSDRGINHFKDVKFLFQLNYSLLLSTSS
                     VLLYLNRKKLVTRDQVREITSLIKWMIISVCVMALLFFDKAFVLFHQVFFDNDDWMFD
                     YRTDPIISFLPETFFFLCFLLIVTISVSTLTTIHHLFNKEERTL"
     gene            10925..11728
                     /locus_tag="LP327_001834"
     CDS             10925..11728
                     /locus_tag="LP327_001834"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL1725946.1"
                     /translation="MREPYDRVFISFGPFTIYWYAIFIVFGIIIGYFVANRRAKRAAL
                     PEDTIVDLLLYGLPVSIISARLYYVLFELPLYIDDPLSILKIWEGGLAIHGGLIGAVL
                     TGVIYCKKKNLSFWNVADVVAPSIVIGQIIGRWGNFMNQEAYGEIVDIEFLQSLKLPS
                     FIIDQMYIDGAYHHPTFLYESLWNIGVLIILLLVSRNRMFFGQIFLIYVSLYSVGRFW
                     IEGLRTDSLMLTANLRMAQVLSIVLLIGSILTYIYLKKSKEEDLHGSIT"
     gene            11712..11936
                     /locus_tag="LP327_001835"
     CDS             11712..11936
                     /locus_tag="LP327_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HBL1725947.1"
                     /translation="MEALLNLLPLLLLLICPISMMFMHKGHGHAGHHHMHGEDHSKHT
                     GSENDIASLKIQTEQLKEEVATLKNLVKER"
     gene            11939..12118
                     /locus_tag="LP327_001836"
     CDS             11939..12118
                     /locus_tag="LP327_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HBL1725948.1"
                     /translation="MQWLLLLLCPIMMIFMMRGMHSGSHNEKDDEQLKQDLENLKEQN
                     DILSSELSDLKSRLK"
     gene            complement(12232..13782)
                     /locus_tag="LP327_001837"
     CDS             complement(12232..13782)
                     /locus_tag="LP327_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HBL1725949.1"
                     /translation="MYTNYNMNQLTLDLSTSFSPKENHVAVFIHELVEALQINDPYLF
                     GRPREYDLGAMMKVVLFAYTRETFTSRKIERLAEENLYARWLTQERVPTYRTIARFCV
                     SNDVQELTNKGLDQLTEYLRARNLIDDALFIDGTKILADANKYSFVWKKNTIGFDQMN
                     REKLLTMMTELKEAYALKQIPEGTTLTVDMIDELLTRMELRLDELENEVEATKRLSPN
                     PAKQQRRTLKNQVRKLNAKREKLLEHQTQFSIYGTRNSYSKTDHDATFMRVKEDHMKN
                     GQLKPAYNLQISTCNQFVLGYDVFQNPTDTKTLKPLLEKMKTAQKSPHYLVADAGYGS
                     ESNYRYIEDELPQHTALIPYGTMFKEQSKKWQTDDRKVMNWVYEPNEDFYIDPKGVRF
                     NFHAYRQRTDADGFVRDFKEYQAEKTDANQALIPEALTPKGNRRKITVNPSWEYHKEK
                     QKERLSTPDIQKIYGRRKVDVETVFGFMQACSGFTRYTVRGLEKVRKQTGLLITAINM
                     MKLTKIGT"
     gene            complement(14493..15776)
                     /locus_tag="LP327_001838"
     CDS             complement(14493..15776)
                     /locus_tag="LP327_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HBL1725950.1"
                     /translation="MVGVITVDNTYLTDVFNQYKLRENLLYQTWFIKSEKRLKAFKKV
                     RKYVQVLIKHVNNGTFPTDFKNSALEDIMNVIAEQTEIFKGAKHAFMWKPKLRIPDIY
                     ENRENQLAFSLCLDQILKANQEIKMLFAVNILAEKKIRGLGPAVANILYFLEPTIFPP
                     FNTAIVNGYNYLTGSKIRLGKWEDYFKLRDGMIELNCLGGLFSKDLGAISAFLFDIGK
                     LNYIVRENEEEYVRISESNTQKLIKQKLEKEDGKNVHQKIQYILATLGNEIGYHAWIA
                     SNDHNRIVDGNRLGDVSIPLLPETIYQLPNPLSRTIGLIDVIWFSKNGEPICAFEVEK
                     STSIISGMNRMDDLYSIHQENLMMYIVSPDQREEEIRAQFNRPHYMRNQNLHDHLRYI
                     LFTDLEKHANFMKKFGKDFTILAPISHYPSGELVR"
     gene            complement(15955..16362)
                     /locus_tag="LP327_001839"
     CDS             complement(15955..16362)
                     /locus_tag="LP327_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL1725951.1"
                     /translation="MNKTLRLMKPWDYFIITAVIMASFVPYLLFDTNNDENNNRESTD
                     VIAVVKIDGEVVDKFNLSQVNESFEKTYYPQKGKYNIIEVAPGKIRVKEDNSPDQIAV
                     RTGWISRPGEMSICLPHKLIVEILGVEENPLIY"
     gene            16634..18133
                     /locus_tag="LP327_001840"
     CDS             16634..18133
                     /locus_tag="LP327_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL1725952.1"
                     /translation="MKKTFISTSVIVFSLFFSNGLLAKAQTIEEKIKEKDDKIEAIVK
                     NKESAENYLTDLDNKITQLEKEYKKVLVEKNTSEKKLTEINQKITALEERIRLRNKLI
                     EEQARNAQVNQKSDSIISVMVNAESLSDAVTAAIGMTKLISASNEIMQAQIDDKENLD
                     ELKKEAEKKVTDAEKETRSLEEKENDLIEAKLTQSIKINEISASLATEQSEKAKFENQ
                     KQEAEKRREEELKAIAEEKRKEAEAAAILKAQEAEAASIIFENQKQEAEKRREKELKA
                     IAEEKRKEAEAAAILKAQEAEAASIIKDQEKDVSQLADTNDIGIGQENTDTQKEEQIP
                     DETSEQDSNSVNQNEYQDNNSSSDNTQIQSPSSSGWGAPVANIIVTSPFGGRADPTGF
                     SGSFHNGIDMGGTSSTPIMASRSGTVVQASFDGSAGNYIIIDHGDGYYSYYLHLSNYI
                     ATPGQSVSAGQTIGTMGTTGNSTGVHLHFGIATSSNWSGFVDPAPFLGI"
     gene            18136..18824
                     /locus_tag="LP327_001841"
                     /pseudo
     CDS             18136..18824
                     /locus_tag="LP327_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
     gene            complement(19362..19658)
                     /locus_tag="LP327_001842"
     CDS             complement(19362..19658)
                     /locus_tag="LP327_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725953.1"
                     /translation="MANNLHYVTNLESRQFDPCWHQIDAQTKELLLGWEENQVVRDFS
                     LNFIMIDYYNLATSMQRFPNYGMDIDSFKQKSFSGEIQNEESNNLVIPILYEPG"
     gene            complement(19740..20006)
                     /locus_tag="LP327_001843"
     CDS             complement(19740..20006)
                     /locus_tag="LP327_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL1725954.1"
                     /translation="MTSTYASFDKDNLYYDFPSVSGFSSMNNKKYLLMLTRLGYSTSN
                     VRAKRNGGTVITDILLSNQYQVIPNQEIQHQATSGVKIIESEVS"
BASE COUNT         6408 a         3738 c         3241 g         6831 t
ORIGIN      
        1 ttttggaaaa ctttgcaaca gaaccaataa gagaaccaaa aatcagaact actggatgta
       61 gttctgattt ttgattctac aatatttagt gttggtggaa aatctacaga ttttctactg
      121 acactttttt tgtaaaaaaa gatagaaaaa gagatagcct aactggtgga atttagtcgt
      181 ctaaaacaaa accataccaa gaaaggatat ctccatgtat cattgtacta gaaaattgtt
      241 aggtttaaca gatgaaaatt tattcttcga agaagaatgg ttagagacag tagaagaaga
      301 tggttttcgc accaatctta tccatgctaa acttagctat atactaagcc attgccgtaa
      361 atgtggcatc aaaaatgaag gacaaatcat caagaatggc tcacataaaa caaaagtcca
      421 acttttgcct tatcgagcaa ccaaaacaga attacgcttg gtacgtacgc gcttttactg
      481 taaagaatgt cagtcgacat tcaacgctca gaccaatttg gtagatgaaa actgctatct
      541 ctcaaaggaa ttaaaggtac aaattgcctt ggagctggcg aagaatacaa caagaaaaga
      601 aattgccgat cgctattttg tctctgacgt aaccgttctg cgtgtcctgc acgcctgttt
      661 aaaaacgtat catcctcgtt ttgatacgtt accttcggtc ctttgttttg atgagttcaa
      721 atcgatgaag tcctgcagtg gaaaaatgag ttttgttttt atgaatgggc agacccagca
      781 attaatcggt gtattggaga atcgtcgcct tgctttctta aaaccatatt tccttaaatt
      841 cactaaaaaa gcacgagcga acgtgaaata cgtcgtgatg gatatgaatg ccccttattt
      901 tgaacttgta aaagcggtct tcccaaacgc caaaatcgtg accgatcgct ttcacattgt
      961 ccaacaaatc actcgtacgt taaatcagtt gcgaatcaaa acaatgaaca ggtttcaaaa
     1021 aacggaaccg acaaagtatt gacgattgaa gcgattctgg aaattactcc ttacgcatgc
     1081 ctatgatctg aatagttccg attatcaata tgatcgatcc tttcgcagac caatgacgca
     1141 aaaagcgatg gtcgatgagc ttttgagtta cgacgagcaa ttaaccaggg cttacgagac
     1201 ttgccagctc ctcctctatc actttaaaca caaggacaac caaagttttt tcgatacgat
     1261 caacagctta gatcaatgcc ttccacaatg gttttgtaag aagctgacgt ttttaaataa
     1321 atacaaatta gggattcaat atgctttaaa acccaggtat agtaatggtg cattggaaag
     1381 aacaaataat aagataaaag tgataaaacg agtagcctac ggctatcgaa atttccacaa
     1441 ctttcgagca agaatttatc tcattcaagg cttgatcttc caagtaaaac aaaaaccagt
     1501 taagcactct gcctaactgg ttatgactaa attttcagtt gtctctctac cagcactaaa
     1561 tgtcgaagag ccaaaaatct gagccagaat gaggttcgtt ctgacccaga tttgattttg
     1621 cacaactcat cagtccttat taacaaagag ctcatattcc tcatgttgag aatacgaagg
     1681 ccatgtacca gcttattggt ttagtatata aaattactca ttattttcgt catattccat
     1741 ctcatcttca gttacccaca tatgattctt aacttcttct tgaccatcgg ttggagtgta
     1801 attaacaacg taaacagttc ctgtaataga tttatctact tttgcttcag ctcccatcat
     1861 cccatccatg tgatcggcat tcaagataac agtatcgccc tcatttgcta cagtctcagt
     1921 atcttttaac tcttcttgta ctacccatcg atgattgtta accattggtc ccccagtttt
     1981 tggctcatag cttacttcat acattgtagt atcaaaggcc cctacaactt gagcatttgc
     2041 acctttcatc ccctccatat gatcgcctag cagtataaca ttacttccca ccgggaattt
     2101 agggttcgca gcatctttca ttgaagaagg aaccatacct tcgtgattca ttccctccat
     2161 actattcatt tcactagacg ctgtagaagt cgattcctcg tttgtgcttt tagaattatt
     2221 cgattcactt tgattgctac acgctcctaa aaatacaata attccaagac ttgcaaatac
     2281 taattttatc atctttttca ctgtcttcat cctcctttaa ttaatcacat ttaggtcatc
     2341 tcataaattt gtaatccaaa aacttctttt ttaattgtag ctattgataa gtccttctta
     2401 gaaaagatta ctacagaatt tgataagata tctaaatgaa ctttttcgac actatctatc
     2461 gaaatcagtt gaaaaaatat tttttttgcc caacattcac atactagttt agcaattttt
     2521 atctgttttt gaaacaaata actctcctcc tttattgaaa tggtaacttt ataacaaatt
     2581 tagttcctag acctaattta ctttttacag ttatactacc tttatgcgct tcaacaacgc
     2641 tctttacaat agctaatccg ataccttgcc cccctaaagc tctacttctg gaggggtcag
     2701 ccatataaaa tctatcaaaa atatgaagtt gctgatcttt ttctataccg atgccgttgt
     2761 cttcaacaat taaaagaaca tggtctttat ttttacttat agaaaactta atggttccct
     2821 ccttttgcgt aaatttaata gcattattca aaagattgat aacaacttgg ttcattttat
     2881 ctttatctgc agatatattg atgttatccc cttggatttc aacatgaagg ttttttgact
     2941 caattttgct ggcgaaattt tcaataacgg attcaatcaa cttatatatg ttaaactcag
     3001 ttttatttaa atgatcatat tttgcttcaa ttttgttgat tacttctata tttccaatta
     3061 aacgagttag acgatctatt tcatcataac aagattgtag gcgctcggga gttattttcc
     3121 agactccgtc aatcattcct tccacattgc ctttaagggt agctaatgga gttcttaatt
     3181 catgtgaaat atcagtagtt aatctttttc gaagtccatg ctgtttctcc aactgattac
     3241 ttaaatcatt tagggaatca atcaaggaat taatttcaat aattgaagtt tcttgtggga
     3301 ttttatcagc atattcccca cgggttaatt tatgtgtaaa gtcgcttaca tgctttagtg
     3361 gtaatcctaa tctagcagag atccacgaag caagtataaa tgatacaaga agagctgcaa
     3421 tagcaacata cattaaactt tgtttcatac tggagataaa caaggcatcg tgctcagtat
     3481 aagcaaaagg tccgaaataa taaaacgtta cttttccaaa ctcgtttcca tcattaatta
     3541 acttcttttt cgcttctatt gtttcgtcta gtttcacact ttttttctca gaaactttca
     3601 aggcattttt tttcagtttg tcctgtgctg acttcaaatt ttttccttta agttcccaaa
     3661 ccatgttgcc ttgttcgtct tctatagaaa aataaatatt attttctaat gctttctcag
     3721 aaagtgctgc aagttcctcg ctgttccatg tttctccact atttaaccac aataaatcaa
     3781 ttgtatatac atactgattc aacaaatctt cttgtcgctc ttctacgtat ttactaaaat
     3841 gattgttcgt caaacttaat gcgactaaac taatcgatcc tattattaat gttgcgattg
     3901 ctaaaaagga aagaactaat tgagttttta tcgttctttt tctcattagt ggccaccaaa
     3961 tctatatcct gtaccatgga ctgtcaaaat atacataggt gatttagcat cattctcaat
     4021 tttatgtctt aagtttttta tatgagaatc gatgctccta tctagtccat caaattctat
     4081 tcccttaact ttctctatca aatcactacg agaaaagact ttattaggtg tggatattag
     4141 ggcgtgtaga atatcaaatt cagttgttgt gaggtttaat tccacttgat gtagataaac
     4201 ttggcgactt tcaggatata ctatcaattc cccattactt gaagaaagct tattaggatt
     4261 tgataaggtc tcagttctgc gcaatacagt ttgtattctc gcaactaatt ctttgggact
     4321 aaatggttta atcatatagt catcggcacc taacttgaga ccagtaagaa tatcatcctc
     4381 atcagactta gctgttaaca ttataattgg tacatttgat acttctctga tttttctaca
     4441 cacagtcaat ccatcagtat ccggaagcat taaatctaac acaactaaat caggattatt
     4501 tttctcgaac ttttccaatg ccattgcgcc acttattgct ttataaactg aataatgatt
     4561 agcaactaga taggcatcaa taatttctaa tattttttct tcatcgtcca caataagaat
     4621 tttcatagac aatcatcctt cttaaattat tttctattta atttccattt gtcccatcat
     4681 tccatactct tcatgttcta atatatgaca atgatacatg aaaatacctt cacggtcgaa
     4741 tttcacaaga agttctactg tttcatccgg atttaccagc acagtatctt tccatccttg
     4801 ctcgtttagt gcgggctgat ttccatctct agataaaatt tgaaactgaa caccatggat
     4861 atggaacgga tgaatcatgc ctcccatcat actactaata ttgttaactt cccaaatctc
     4921 ttgagtacct aattttttat ataaatctat tctttccata tcaaattgct tattgttgat
     4981 gtttaccatg tgagacattc ctcttaaatt aatactttgt cttgctaaat cttccaattt
     5041 cttttcatct aaagtactaa tagtatttag actatcagag ggagtaaact ctttgttttc
     5101 tatcgtattc tcaattctca ttgtcaaggc aaccaaatta tttgctagta aatgaattac
     5161 ctcacctttt ttataattct gagtatccac taatatttct gccctttccc cgggggctaa
     5221 aagtagttta ctaagtttaa cagaagtatt cagaaaacca ccatctgtag caatttggta
     5281 gaatgattca tctttatcca aagtaaaagt gaagtttctt gaattagatc cattaacaat
     5341 tctatacctc atccatcggt tttttatctc aatatatgga ttgatagtgc cattagtaag
     5401 caacgtctcc ccttttgttc catcagagtt aaaatcattt tcatagttaa tttgattagt
     5461 ggagctgaaa gatttatctt gcacaatcaa aggaatgtcg tcgacaccat atttggaagg
     5521 taaatctaat gattttgagt tgtcatcatc aatatacatc aaacctgcta aacccttata
     5581 aacttgagat gctgtttctc cttccggatg gggatgaaac cagagtgtag aagcttcttg
     5641 atcaacctcg aatgttacac tttttttctg tcctgtttct ataatctgat gtggacctcc
     5701 atctgcatca gaagctactt tcaaaccgtg ccaatgaaaa gatgtgctag catcaagatt
     5761 attgtttgta ttgatagtaa ctttttgacc ttttttcagt ctaataactg gtcctaaaaa
     5821 atccccgtta tagcctaatg tttcagtttt ttctccatcc attatttgaa cttctccgtt
     5881 ttgagcaact atatcatata tcacattttc tttgcttttt gaagatggtt caagaacagg
     5941 gggaatcaat agttgtctct ccgtttggtg tactaccttc aaatcagctt cttgattatc
     6001 ccccatcata ttggacattc tacttgagat ttgtcgttgt tcttcttgaa agacaacttc
     6061 agcatgctcc tccttgttct gctgttgaat caatatgaaa acaccgccta gggtcaacat
     6121 tgatattgtt gctgataacc aaatatattt attattcttc aacccatcat ctcccatatc
     6181 tattatgtag ttaccataaa aaaaatatgt ggagattttg tgttgattcc cccacatata
     6241 cttatactta tatagatata ttcctagtta cagtatactt tgtatttttt agtagttgat
     6301 ttaagaatgt taaagttaat tttgaatcag catcgaaagt aactatacct ctagccaaat
     6361 caactatttt tacactgact gttggctctg cttccaaagc atttcttact tttgatgcac
     6421 accttgagca attcattccg tcaataacca ctatttgcaa atatttcatc ataaaattcc
     6481 ttctttctta aaaaagtcgc ctatattgga tttactaatc taatcaatct tttaagaatt
     6541 gtcttacaag tatcataagg aaaatttatg gagaaaccat gtatagagtt tgttttagag
     6601 cttttaaata taggatagat acagattgat ttgtttagtg tatgattgca tatcatggac
     6661 atatatcgct aagtatcgcg cccaaaacat aggtttcgag tttcatgaat atcctaacag
     6721 gtagaagtgc tatgatgata ttcgataagc atcctaatat gaaatataag tttagaactg
     6781 ctattttgga tagttcttcc tcctattcaa ataaataatt gttctacata agaatttcac
     6841 aatcctctct accctcctct actaccaagt gaaaatccat ttcaactaat accaaaaacc
     6901 tacattggga ttccaaagga tttttgagtt caaaaaaaca tgaaatgcct ctcagaaatc
     6961 cgtccattgg attcaacata tacttatgca caaaaagcag tgaataaatt taaaaaactg
     7021 gtttttctat atatctcaat ttgaaaaaag tagaatatag aggaactttc ttaccataag
     7081 aattcacata tttaatgtga tgattcacca aacttgtaat gccttataat tcgtattttt
     7141 caacaattga ttaataacgc taattgataa atctgtcttt gttgcaatag ttactaaacc
     7201 ttcttcgaga tttactttcc taacagaaat tgagggattc ttttctaatg catacttaat
     7261 ttctccggcg catcctagac aagccattcc actaataaat aagggctgta aatgttcagt
     7321 cataagacaa tcacctttcc tatttgactt tgaagttttt taagcgaagt gaatttaaaa
     7381 gtactgttat tgagctaaaa ctcatagcaa ctcctgcaat taacgggctt aataaagacc
     7441 tccaaaaaat gttcaaaaca cccatcgcta ttggaacact taacatgtta taagcaaatg
     7501 cccagtacaa atttgacttt atattccaaa ttgttttttt gcttaaatca atcgtggaca
     7561 ttagcaattg cagatcgtcg ttcatcaata ttatatccgc agattccatc gctatatcag
     7621 taccagaacc catcacaaaa cctatatccg cttgagcgag ggcaagtgca tcgtttatcc
     7681 cattacctac cattgcaacc ctatgttctt ttgactgcaa cattttaaca taataggcct
     7741 tttcttgagg taatacctca ctaataacgt tggtgatccc aacttgatta gctattgcct
     7801 gtgcagtaag tttattatca cccgtgagca taaccacatt gaggccaagt ttattcaact
     7861 tttgaattgc taatgcactt gctggcttta tagtgtcttc aatcgcaatt attccgagaa
     7921 agatacgatc atttgcaata tacactggtg tctttccttt agatactaaa ttatctgaaa
     7981 ttaacttaaa tttactaatg tctatattca actgcttaaa gaactcttca tttcctaaac
     8041 ttacttcggt acctttcaat atagcattaa cgcccaatcc agagaaagat tcgaatatac
     8101 taggttcaac cagatcaatc cccacgattt cggcttcttt tacaattgcc tccccaagcg
     8161 gatgctcgga tgctttttca gcagaggccg ctaaaaacaa gagaaagttt ctatccatat
     8221 tttctgcaag tattatatct gttaccacag gttttccttt tgtaatcgta ccagttttat
     8281 cgaataatat tgtttttacg tgatgagttg tttctaaagc ttcaccactt ttaattaaga
     8341 taccattttc agcgcctttt cccataccga ccataatagc tgtccgtgta gctaaaccga
     8401 atgcacaagg acatgttata actaaaactg aaattaagac tgagaatgaa aacgaaatac
     8461 tttgtccaga tagcacccaa agtagactag atccaatcgc aactacaata atgatatata
     8521 caaagtaact tgaaatgata tcagccagtt tttcgattgg agctttcgaa gattgtgcat
     8581 catccactag tttgcttatt tgagctaaag tggtgtcttt accaatatga gttgtttcat
     8641 atcttattac gccattttta tttttactgg gtcctattac tcggtcatta gctttctttt
     8701 caacaggaag attttcacct gtaggcatag attcatcaac taaagactta ccacttatta
     8761 caataccatc cagtggcaaa cgctctcctg gttttgctat gatcacatct cctactaaaa
     8821 ctgattcaat aggaacagtc tgttccttat tatgtctgat agttttcgct gttttgggag
     8881 tcatcctcat taatttttgt attgcttcag atgtcttatc tttcgtcacc aactctaggt
     8941 atttctctag tctgattaac gttaatatca ctgctgatgc tccaaagtag agctcttttg
     9001 tatagagata atttcccaat ataatctgta ttgttgcaaa gatgccgtat aacaaagcaa
     9061 tacttgtacc taaagcaatt aatatgtcca ttttcggatg ttttttcatg agtgaggaaa
     9121 atccaatttt ataatagtct ttattggcca atataaccgg agcggtaagt attaattgcg
     9181 tcatcgaaaa aaaactaggg tttgtcgtag gatcaattat ttcaaatagg ggtaactcaa
     9241 gtatatttgc actagcaaca tatataatag gaattgtaaa tagcattgac gtgataaatc
     9301 ttcgccttag cttctgttgc tgttgctctt gttcttttgt tttatatatt acttgttcct
     9361 catcttcaga aagagcatat agctgatatc caagaatttt gacaacatca cttatttttg
     9421 caaccgctga agggtcagtt gatattaccg agagtttttc tgcttcaaaa tttacgttgg
     9481 catatttgac ataagtcatt gatttaacta cattttcaat tttgttagcg caggacacac
     9541 aagtcattcc acttattcca aataccttta tctcagtttt ttttcgaatc tcagcgtcat
     9601 aaccaatgtc ttgaatagct gttataactg tatgattaga caaaatacct tcatcatatt
     9661 ccaattttag aacctcagtt gttaagttaa ctttagcgaa atatactcca tccaactcat
     9721 gaattttttt ctctattgtt gttgcgcaaa ctgcacaagt cattccataa agattatatt
     9781 ttgaaatcat tgaattcctc cgtgtcagaa cgtttaacca atccgtttaa atagtagctt
     9841 acttctgaga tacaaccttc atccattcca gaaagtaacc tttgtatttc ggtttctaaa
     9901 aattcttgct tcttaatcag tgaagtgtag tatctttggt tttttgaagt gttaaacgtg
     9961 atcacatttt tatctaacaa ccttcttatt aacgtttttg ttgtggaggg atgccaacta
    10021 ttacgctctt gcattccatg tgttattaaa ccacatgtta ccggttcata tctccagata
    10081 aactttaaaa catctaactc tgaattcgat acctgtattt tgtgttcgac caagaccatc
    10141 cctccttagg ctattcataa agtacgttct tctttattaa acaaatgatg aattgttgtc
    10201 aaagtagaaa cacttatagt tacaattaat aaaaagcata aaaagaaaaa tgtttccggt
    10261 aaaaaactga taattggatc tgtacgatag tcaaacatcc aatcatcatt gtcaaaaaat
    10321 acttgatgaa aaagcacaaa tgctttatca aaaaacagaa gtgccattac acagactgat
    10381 attatcatcc acttgattaa actagtgatt tctctgactt gatcacgcgt tactaatttt
    10441 ttcctgttta ggtataaaag aacgctactc gtagaaagaa gcaatgaata gttaagctgg
    10501 aacaaaaatt taacatcttt aaaatggttt ataccgcgat ctgagacgta tagattatca
    10561 aaaaccaatt ctttttgaaa aggattattt aaatagccca ttaaatttct aaaatcttga
    10621 ttaacttgag atctactcca gttttgaaaa tttatatcgc tttgaaagaa taaattgatt
    10681 ttgagcaaac cagggaagtt gattgtcaat gtaatcgcga gggataaagc aaagacaaaa
    10741 aataatagaa atagcatgct ttttttcatt aaattaatat ttatattaga tcactccttt
    10801 taatataatg ataaaaaaac ttgtgtagaa aatgtgtacc aatgtatctg tcaaaaagtc
    10861 tacataaaat ttccataata gcctgctata gtcaaaacaa gttcattagg aaggagggaa
    10921 gaatttgaga gaaccctacg acagagtatt tatttcgttt ggtccattta ctatttattg
    10981 gtatgccatt ttcatagtgt ttgggatcat aatcggatat tttgttgcta atagaagagc
    11041 aaagcgtgcg gcattgcctg aggatacaat tgttgatctg ttgttatatg gattgccagt
    11101 ttctattata tcggccagat tatactatgt actttttgaa ttacctttgt acattgatga
    11161 tcctttaagt atcttaaaaa tttgggaagg tggtctagca atacatggtg gattaattgg
    11221 tgctgtattg acaggggtta tttattgcaa gaagaaaaat ctttcttttt ggaatgttgc
    11281 tgacgtagtt gctcccagta tagtaattgg acaaatcatt ggacgatggg gaaattttat
    11341 gaatcaagag gcctatggag aaattgtaga catagagttc ctgcaatctc tgaaactccc
    11401 atctttcata attgatcaaa tgtatattga tggggcctac catcatccga cttttttata
    11461 tgagtctcta tggaatatag gagtactcat tattttgctt ctggtgtcta gaaatagaat
    11521 gttctttggg caaatatttt taatttatgt ttcactatat tccgtcggga gattctggat
    11581 tgaaggatta cggaccgaca gtttaatgct aacagcaaac ttgagaatgg ctcaagtcct
    11641 gtccatagtc ctattaattg gctctatatt aacttacatc tatctcaaaa aatctaagga
    11701 ggaagattta catggaagca ttacttaatc tcttaccact actattacta ctaatttgcc
    11761 ctatctcaat gatgtttatg cataaagggc acggacatgc tgggcatcat catatgcatg
    11821 gagaagatca ttcaaaacac actggttccg aaaatgatat agcaagttta aaaatccaaa
    11881 ctgagcaatt aaaagaagaa gttgctactc taaagaactt agttaaggag cgatgacaat
    11941 gcagtggtta ttacttttat tatgtccgat aatgatgata tttatgatga ggggtatgca
    12001 ctcaggatcg cataatgaaa aagatgacga acaattgaaa caggatcttg aaaatttaaa
    12061 agagcaaaat gatatcttaa gtagtgaact aagcgatttg aagtcaaggt taaaataagt
    12121 taaaggaggc gtgacataag ttacctagtt acgctctcca aaaaacaagg agcacttgga
    12181 aatcatgaga tttttcgagt gcttcttgtt tttttattca ttttgagtag ttcaagttcc
    12241 tatttttgtc aatttcatca tattgattgc cgtaatgagt aaacccgtct gttttctgac
    12301 tttctctaag cctcgaaccg tgtagcgagt gaagcccgaa caagcctgca taaatccgaa
    12361 gactgtttcc acgtcgactt ttctccgccc atatattttt tgaatgtcag gcgttgataa
    12421 ccgttctttt tgtttttctt tatgatactc ccaagacgga tttacagtga tctttctacg
    12481 attccccttt ggggttaagg cttcaggaat gagcgcttgg ttcgcatccg ttttttctgc
    12541 ttggtattct ttaaaatctc ggacaaagcc atccgcatct gttcgttgac ggtaggcatg
    12601 aaaattgaat cgtacccctt tggggtcgat gtaaaagtcc tcattgggct cgtaaaccca
    12661 attcattact ttccggtcat ctgtctgcca tttcttactt tgttctttaa acatcgttcc
    12721 ataaggaatc agtgcagtat gctgaggaag ttcatcttct atatagcggt aattgctttc
    12781 tgaaccataa cctgcatcgg cgaccaaata atgaggggac ttttgtgcag tcttcatttt
    12841 ttccagtaat ggtttcagtg ttttagtgtc tgttgggttt tgaaagacat cgtagccgag
    12901 cacaaattga ttacatgtag agatctgcag gttatacgca ggtttaagtt ggccattttt
    12961 catgtggtct tcttttacgc gcataaatgt cgcatcatga tcggtttttg aataactgtt
    13021 tctagtccca taaatagaaa actgcgtttg atgttccaga agtttttctc gctttgcatt
    13081 tagtttgcgt acttggtttt tcaacgttcg ccgttgttgt ttcgctggat ttggcgaaag
    13141 acgtttcgtt gcctcaactt cattttctag ttcatccaga cgcagttcca tccgtgttaa
    13201 cagttcatct atcatgtcta cggtcaaggt cgtcccttcg ggaatctgtt tcagggcata
    13261 tgcttctttt agttccgtca tcatagtaag caatttttcg cgattcattt gatcgaaccc
    13321 gatcgtattt ttcttccaga caaagctgta tttattcgca tccgccaaga ttttggtccc
    13381 gtcaataaac aacgcatcat caatcagatt ccgtgcacgc agatactctg tcaactgatc
    13441 taaaccttta ttggtcaact cctgtacatc gttggataca caaaaacggg cgatagttcg
    13501 ataagtcgga acccgttctt gcgtcaacca gcgagcatat aggttttctt cagctaagcg
    13561 ttcaatcttg cgacttgtaa aggtttctcg cgtatacgca aaaagaacca ccttcatcat
    13621 ggcacctaaa tcatattctc ttggtcgacc aaaaagatag gggtcattaa tctgaagcgc
    13681 ttcgaccagt tcatggataa aaacggcgac atgattttct tttggagaaa atgaagtaga
    13741 tagatccaaa gtgagttgat tcatgttata attagtatac attttgttta gtcctttcag
    13801 gattaaatga tggtgcattg gtgggaaccg gtgctccttt ttattttacc caaaaaaccg
    13861 caagaaatga acgctttctc gtcatttctt gcggttttct tctatggact tatgtcacag
    13921 tccctttaaa attcactaaa ttcatggttc agaatagaat tgattctctc aaaatattta
    13981 gtctaacttt actctttata aaacgaataa ggcatgcgtg gtttataatc tttctttatg
    14041 tatttctgac accaaagaaa atgatcctca tcattccatc cactcgttgc attccaccaa
    14101 gagtacataa catcccctgg tgtatagggt agtccataca ccaggggatt ctagaagtaa
    14161 gatttattgt ttctttttta agtgcaaata aaaccattat cttcgatgaa tataaggaaa
    14221 ataccttttt agataacatt tctaggttat gaatagatag tattcactct atccgcaatt
    14281 ttcttcaacg aaaagttact aaaatggaag catgggcagc attaatatga tgattttctt
    14341 ctggacgttg ctaagacatt caatagcaat gagcgccatt ataaatgtgg aagctactcg
    14401 taaaaaaagg gaagattgat ttccaattct gcccaaatgc ttatttatac tacaattaac
    14461 cgtattaatt tcaacttctt tgtccagaat aactaccgaa ctagttcacc acttggataa
    14521 tgagatatcg gggctaaaat tgtaaaatct ttgccaaatt tcttcataaa attagcatgt
    14581 ttctctaaat ctgtaaatag aatgtatcgt aagtgatcat gcaaattttg attacgcata
    14641 taatgtggtc tattaaattg tgctcgtatc tcctcttcac gttgatccgg tgagacaata
    14701 tacatcatca aattttcttg atgaatcgaa tacaaatcat ccattcgatt catcccagaa
    14761 attattgacg tcgatttttc aacctcaaaa gcacatatcg gttccccatt ttttgaaaac
    14821 caaataacat caatcaaacc aatcgtccta cttaacggat ttggtaattg atagattgtt
    14881 tctggtaata gaggtataga cacatctcct aagcgattcc catctacaat tctattatga
    14941 tcgtttgagg caatccaagc atgataacct atctcattac ctaaagtagc taagatatat
    15001 tggatttttt ggtgcacatt tttgccatcc tctttttcca acttctgttt tattaatttc
    15061 tgggtgttac tttctgaaat tctgacatac tcttcttcat tttcgcgaac aatataattt
    15121 agtttcccaa tatcaaataa aaaagctgag atagcaccca aatctttcga aaaaagtcct
    15181 cctaagcagt tgagttcaat cataccatca cggagtttaa aatagtcctc ccatttacct
    15241 aagcgaattt tcgagcccgt aagataatta tatccattta caatagctgt gttaaaagga
    15301 gggaaaatgg taggttctag aaaatataaa atgtttgcga cagctggacc aagccctctg
    15361 attttctttt ctgctagaat atttacagca aataacattt taatttcttg attagcttta
    15421 agaatttgat caagacaaag tgaaaaagct aactgatttt ccctattttc ataaatatcc
    15481 ggaattctca atttaggttt ccacataaat gcatgcttgg ctcctttaaa aatttctgtt
    15541 tgttctgcaa ttacattcat tatgtcctct agggctgaat ttttaaaatc tgttgggaac
    15601 gttccgttat tgacatgttt gataagtact tgtacgtatt tcctaacttt tttaaacgct
    15661 ttcaaacgtt tttcactttt gataaaccaa gtttgataca gtaaattttc gcgtaattta
    15721 tattgattaa acacatctgt taaataagta ttatccactg taatgacacc taccatccct
    15781 attaatattt tataaactcg ttagctctaa tatggctttc tatatgcaaa attccgtaac
    15841 caccttaaat gaagaataat gtaaacaggt tttagcgatt ttagttaacg gtctttcctt
    15901 agattactcc gtccatgtct ctataatagg attcgtggta cttattagtt catactaata
    15961 aattaaagga ttctcctcta ctcctaaaat ttcaacaatc aatttatgag gcagacatat
    16021 actcatttcc cctggtctac taatccagcc tgtacgaaca gcaatttgat caggactatt
    16081 atcttctttt accctgattt ttccaggagc tacttcaata atattgtatt tccctttttg
    16141 agggtaatat gttttctcaa aagactcatt aacttgtgat aagttaaatt tgtctacaac
    16201 ttctccatct attttaacaa ctgcaattac atctgtactt tccctattat tattttcatc
    16261 attattagta tcaaatagta aatatggtac gaatgatgcc ataatgaccg cagtaataat
    16321 gaaatagtcc caaggtttca tgagtctcaa tgttttattc actaaattcg ttctcctgta
    16381 ttaacttttg atatcatact ttacagttgt gtgaatttta tgtaggtatg tatatgctcc
    16441 ataaaatctc cataagtttt agttattttg aagctgtcta ttaaattgaa atatagcata
    16501 tggatcctat tactttttgt atagtaattc caatttcaat tcctaataga acctgtagca
    16561 acgataaaat agagaggaga atattatgta aaatgtttct tcacaaataa aataagggaa
    16621 gtgaaaaatc aatttgaaaa aaacatttat atctacctct gtcattgtgt tttcgctatt
    16681 ttttagtaat ggtctcttag ctaaagcaca gactatagag gaaaaaatca aagaaaaaga
    16741 tgataaaatt gaagcgattg taaaaaataa agaatcagct gaaaattatc ttactgattt
    16801 agataacaaa ataactcagc tagaaaaaga atataaaaaa gtgctagtgg aaaaaaacac
    16861 atctgagaag aaacttaccg agattaatca gaaaattact gctcttgagg aaagaatacg
    16921 ccttaggaat aaacttatcg aagagcaggc taggaatgca caagtaaatc aaaaaagtga
    16981 ctctataata tctgtcatgg taaatgccga atcattatca gatgcagtca cagcagccat
    17041 tggtatgact aagcttattt ctgctagtaa tgagattatg caggcccaga tagatgataa
    17101 agaaaattta gatgaattaa aaaaagaagc tgagaaaaaa gttacagatg cggaaaaaga
    17161 aactcgatct ctcgaagaga aagaaaatga ccttatagaa gccaaattaa ctcaaagtat
    17221 aaaaatcaat gagatatccg cttctttggc gacagagcaa tccgaaaaag caaaatttga
    17281 aaatcaaaaa caagaagctg aaaaaagaag ggaggaggag cttaaagcta tagcagagga
    17341 aaagagaaaa gaagcagaag ctgcggctat tttaaaagct caagaggcag aagccgcatc
    17401 tattatattt gaaaatcaaa aacaagaagc tgaaaaaaga agggagaaag agcttaaagc
    17461 tatagcagag gaaaagagaa aagaagcaga agctgcggct attttaaaag ctcaagaggc
    17521 agaagccgca tctattataa aagatcagga aaaagatgta tcacaactag ccgataccaa
    17581 tgacatagga ataggtcaag aaaatactga tacacagaaa gaagaacaga taccagatga
    17641 aacttcggag caagattcga actcagttaa tcaaaatgag taccaagaca acaatagttc
    17701 ttccgataat actcagattc aaagtcctag ttcttctggt tggggcgcac ccgtagccaa
    17761 cattatcgtc acaagtccat ttgggggaag agctgatcct actggatttt ctggatcttt
    17821 tcacaatggt atagatatgg gtggaacttc ttcaacgcct atcatggcat caagatcagg
    17881 aactgtagtg caggcgtcat ttgatggatc cgcaggtaat tacattatta ttgatcacgg
    17941 agatggttac tattcatatt accttcacct aagtaattat attgctacac caggccaatc
    18001 agtgagtgct ggacagacca tcggtaccat gggaactaca ggaaattcaa ccggagttca
    18061 tttgcatttt ggaatagcga ctagctcaaa ttggtctgga ttcgtagatc ctgctccatt
    18121 tctaggaatc taaaaatgat gaaaaaaatc aagcatcttt ctttaaattt acttctaatt
    18181 agcctttttc ttataggctt agttttaata tttaatttcc aaattcgatc ttacttactg
    18241 aataaacaag tccacaatat gacatagcaa attattcaca gaaagatttg gaagacaatc
    18301 tgaaaaggaa aggaaacttt gattttgatt ctgttgaatc tatatcaaat gagttagtat
    18361 taaaagcaca attcaagaat aatgatttgc ctataatagg tggtatcgct atacacaatg
    18421 taaaattatc tttaccaata tacaacggat tggacaacac ctcgctcctt tctggggctg
    18481 gaacaatgaa accagatcag cagatgggaa aacgaaatta cgctttagca agtcatagtg
    18541 ctaaagataa aactatttta ttttccccat tagagtttat ttctctaaaa gaaaaaatat
    18601 atgtaacaga cttaaagaat atttatattt accaagtgtc ctttaaagaa aaagtggatc
    18661 catcaagagt cgaagtcatt gatgatattc ctaataaaaa tatgatcact cttgttacat
    18721 gcggagattt ggctgctgta acgagattga ttgtacaagg tacactgatc gatacagttg
    18781 aatttaagaa tgctaatcaa tatatccatt ctttatttag ctaatataaa aagtcttatc
    18841 aatcctgaca gattacgagt agtacttttt attaaagaaa gaaggtgctt tctaaattca
    18901 aattggttta tggtcataat aaattaaata gggaaacgaa cgcgattata agttcgtttc
    18961 tctattttgt atgagtgtct ttaatctaat tgaaaagagc cctttttgat ccaacctcaa
    19021 gaaaattttg aaaaaatctt actatggagc taactagtaa cgcaccctat ttaatcattt
    19081 aggaatttgc ttatatcccc cttgtcattc tatagtttct agggaagatt tagattgtga
    19141 ttttcctaag gaatatcctc tgttttcttg atataatcca gaaaaagtaa aaataaaaag
    19201 cacccagttg caatactaga gattaccaag cctaccaaga atcctggtgc gaaaaattct
    19261 ccagcaattt tcatattact atcttttggt aagttagaca attgaatcaa atcgtcaaaa
    19321 gctactttgt aaggatactc ttccccgtcc acagtaaaaa cctaacctgg ttcatagaga
    19381 ataggtataa caagattatt gctttcctca ttttgaattt ctccagaaaa gcttttctgt
    19441 ttgaatgaat ctatgtccat accataattc gggaaccgtt gcattgatgt cgccaaatta
    19501 tagtaatcaa tcataataaa atttaatgag aaatcacgaa ctacttgatt ttcttcccaa
    19561 cctagcaaca attctttcgt ttgagcatca atttgatgcc agcagggatc aaattgtcga
    19621 ctttccaaat tggtcacata gtgcaaatta ttggccaagt tgacgggaaa ataaacataa
    19681 agtaactcat cttttgttac ttctagaacg tcaaatgaaa aagaagcaat atctgaggtt
    19741 taactcactt cgctttctat aatctttaca ccgctagtcg cttgatgctg gatttcttga
    19801 ttaggaatta cttggtattg gttgcttagc aaaatatccg taataactgt accaccattc
    19861 cgtttagcac ggacgttact tgttgagtac cctaatctcg ttaacattag taaatatttt
    19921 ttattgttca ttgaactaaa tcctgatact gaggggaaat catagtagag attgtcttta
    19981 tcaaaggatg cataagttga agtcactttt tttagtggtt cccaatattc ttgatatact
    20041 tcaaattctg ttagagtttg gttgattgtc ttggaatggc aacagttttt ttgacaaatt
    20101 ttataaggtg cagaacttct ttccgtatgc tattccgatt gtccttgaca atgagcctcc
    20161 tcggatgtga cgatcttcgg caggagctaa cagttaaagt tagactgcct cgctagcg
//
LOCUS       DAFEHS010000050        19814 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFEHS010000050
VERSION     DAFEHS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.051"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(8..1376)
                     /gene="nhaC"
                     /locus_tag="LP327_001844"
                     /pseudo
     CDS             complement(8..1376)
                     /gene="nhaC"
                     /locus_tag="LP327_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
     gene            complement(1485..2906)
                     /locus_tag="LP327_001845"
     CDS             complement(1485..2906)
                     /locus_tag="LP327_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1725955.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3149..5026)
                     /gene="tdc"
                     /locus_tag="LP327_001846"
     CDS             complement(3149..5026)
                     /gene="tdc"
                     /locus_tag="LP327_001846"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL1725956.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5317..6573)
                     /locus_tag="LP327_001847"
     CDS             complement(5317..6573)
                     /locus_tag="LP327_001847"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBL1725957.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6685..6890)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7153..8454
                     /locus_tag="LP327_001848"
     CDS             7153..8454
                     /locus_tag="LP327_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL1725958.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8523..9122)
                     /locus_tag="LP327_001849"
     CDS             complement(8523..9122)
                     /locus_tag="LP327_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL1725959.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9376..10257
                     /locus_tag="LP327_001850"
     CDS             9376..10257
                     /locus_tag="LP327_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1725960.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10270..11880
                     /locus_tag="LP327_001851"
     CDS             10270..11880
                     /locus_tag="LP327_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBL1725961.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(11957..12616)
                     /locus_tag="LP327_001852"
     CDS             complement(11957..12616)
                     /locus_tag="LP327_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL1725962.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12899..13564)
                     /locus_tag="LP327_001853"
     CDS             complement(12899..13564)
                     /locus_tag="LP327_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBL1725963.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13711..14316)
                     /gene="dhaL"
                     /locus_tag="LP327_001854"
     CDS             complement(13711..14316)
                     /gene="dhaL"
                     /locus_tag="LP327_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBL1725964.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14344..15354)
                     /gene="dhaK"
                     /locus_tag="LP327_001855"
     CDS             complement(14344..15354)
                     /gene="dhaK"
                     /locus_tag="LP327_001855"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBL1725965.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(15329..15775)
                     /gene="dhaM"
                     /locus_tag="LP327_001856"
     CDS             complement(15329..15775)
                     /gene="dhaM"
                     /locus_tag="LP327_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBL1725966.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            complement(15814..16773)
                     /locus_tag="LP327_001857"
     CDS             complement(15814..16773)
                     /locus_tag="LP327_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL1725967.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            16943..17263
                     /locus_tag="LP327_001858"
     CDS             16943..17263
                     /locus_tag="LP327_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBL1725968.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(17375..18244)
                     /locus_tag="LP327_001859"
     CDS             complement(17375..18244)
                     /locus_tag="LP327_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL1725969.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(18340..19644)
                     /locus_tag="LP327_001860"
     CDS             complement(18340..19644)
                     /locus_tag="LP327_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL1725970.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(19641..19802)
                     /locus_tag="LP327_001861"
     CDS             complement(19641..19802)
                     /locus_tag="LP327_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725971.1"
                     /translation="MKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
BASE COUNT         5638 a         4099 c         3622 g         6455 t
ORIGIN      
        1 ccttccttca attttttaca cgtcgacgat tcaataaaaa accgcctaat attgtaaaga
       61 caggtgcaag aatcgagaaa aaggcgaatg gcaagtaatg caacgcgcta actttcatcg
      121 ttcccataat gaatgtgccg ctgactcccc acggtacgag agaattgacg gtcgcacctg
      181 aatccgctag tgttcgtgtc aaataggttt ttgataagcc aacgtcatca aaagaacgct
      241 tgaatgtttc tcctggaaga atgatggaca gatactgttc tccaacgatc agattgattc
      301 cgatacagct caaggcagta aacccgacta aacgagaagg tcggtccatc ttttctttta
      361 attcttgaac gatggtatca acgatcagat atttgatcag taacccacct aatgccaatg
      421 caagtaaaat caaactagca gaacctaaca tactcatgat cccgcctcga gaaagcagtt
      481 catctagctt tggatcaggt gtatttgcga cataaccatt catcaagatg tctgctgctt
      541 ttccgaccgt caaacttttc tcatggataa aagaaagaac gagagcagct gtcgatcccg
      601 ctagtaatgt agggatagcc ggaatttttt tgattgcccc ccccaaataa aataactagt
      661 ggaatcaatg tccaaggaga gatccaaaag ctcgctttca tgttttggat catttcattg
      721 atactagcta gatcggctga atgactgtta tgtcctaata tcgtataaaa aatcaacgaa
      781 aggacgaatg ctgggaaatc cgtataacgc ataccagcga tatgttcgaa tagattaact
      841 cctccaatac ccgctgccaa gttggtcgta tctgataaag gcgagcagtt attccctaaa
      901 aaggcaccgg aaacgactgc gcctgtagtc attgcatcat tgaatccaag taaatgaccg
      961 atccccaaaa aagcaattcc aatcgtcgaa atcgttgtaa atgaactgcc gacagtcact
     1021 ccgacaattg cacaaacaag aaatgctgtc ggtaaaaaga aacgcacaga aacaagttta
     1081 aatccataaa ccataatcgt agggatggtt ccagataaaa tccatgaagc aactaagaca
     1141 ccaatcaaaa gaaaaatcag gattggaatg atccctggag tgattccttt gatgatcccg
     1201 tcatggatct catcccatgt gaacttttta aatctgccat aaaacaacaa acacatgaaa
     1261 gctgcaagga tcgggatatg tggcgagagc tggaatccga tgatcagtac tcctaggagt
     1321 gccaacaaca cgatcaatac agcgatactt tcttttaaag aaatattttt attcatcgtc
     1381 gatctcctat ttcaaatgaa aaggccctgc catgaaaaaa ctattcactt ggcaggacct
     1441 cctactaaat cagcgacaaa acgtcggctg aaagacttat ttttttattt ttcttctaaa
     1501 cttttttcta cctcaatccc ccaagaatct ttcttgaagt gattgatgat caatggaata
     1561 acgaacatga ccactgtgat agctgctact aataatgcat cactcaatgt cgctgtacct
     1621 aatgtagcaa atacgacaac gatgatagag aagatcagca tgcagctgac tacccaagct
     1681 aatccgtttc cgtttttacc aattcggtaa ggacgttcga attctggatg tttcttacgc
     1741 attttaagca atgaaacagc aatcaaaatg tacacgatac aataaataac agttgtagca
     1801 tttgtaagtg tcaagaatac agcacttacg tcatccatta caccataaag caaagcaaat
     1861 aatgaaataa cgattgattg tgttagaacg acatttctag atacgccgta tttgttttct
     1921 ttatggaatc cgaattttgg tggaaggaat ccttcacgag ctacttggat gattgttttt
     1981 gatggtccag taacccaagc tgataattgt aaaagcacac caataaatac catgaagctg
     2041 aagatatttc cgatcacttc tggtaagcct aggatttgac agtcaatcaa gataggctgc
     2101 gtgatatttg ctaattccat ttttccatct ggtacgacat ttgctacaag catcgcattg
     2161 atgacattca ataaaactaa tccgatcaaa gcgataaata cgccttttgt ataatttttt
     2221 gttgcgtctt tcaaacgagg catgtaaaca gaactcattt cgatcccaac aaaaataaat
     2281 gtgataccag ctaagtattt caatgaatca atatgttcca agtcagggaa agcttttgac
     2341 catgaaaagt cacctagata gccgtttgag actagtccga ctttgaaggc tgccaaaaca
     2401 cctaaaacga acatgatgac tactgggata tataccccta gccatacacc gatgttcccg
     2461 ccgacttttg ccatatcgaa tttcaagttc aagatcgtga tgatccagta gataactaaa
     2521 atacaaccta aaacgaacca gtgattattg ccaagttcta cattaccaaa tgtgtttcct
     2581 aacaatggtc ctaaagtaga agcgaccatt accatacctg ggaacatttg cacccataat
     2641 agccatgcaa cgacaaatcc ccatttgctg cccagtgctg tttttaccca taactgagga
     2701 ccgccttctt gaggaagcat cgtggaaagc tcaccggaaa ccattgatat cggaccggca
     2761 aaaaacaaaa ctgcaaaaat tgaataaaaa atcaatgtcc aaccgactgc ggctaacgta
     2821 ggaatactcc gaaccgttgc acacagtgcc attgtcagac cgataaaggt cccaagaccc
     2881 attttactta cttcagattt actcaccgtt gacactctcc ttgatttttt ggataaaata
     2941 acggtgtctt tgaagtagtt tcagcaataa acgaaacaat tcagcgaata tttgagggga
     3001 ttctttgagt ttattgcttg aagggtttct tcaaagacaa cctcaaaaca aagggatcga
     3061 agagcttagg tctccccttg ctcttcagat ccaaacatgc gtcagaaaca gttagtattc
     3121 aagctgtttt ctaacatgat taccttgctt attttgcttc gcttgccaat aattggtctg
     3181 cgtagatttt ttccaatttt tcttgaagag ctgctttgat tttttcagca tattcttcaa
     3241 agtgttcttc tttgttcatg tatggtgtca ttacgctggc acgcaataca gttacttttc
     3301 cggcacggtt ccattcttca tctgagaatc ctagttggtt cacaaattgt aatgggctgt
     3361 tgccatagtc tgggatagca aagtcagtat gtgaagttaa gaactcatta ccgtaaatgc
     3421 ttcctttcac gtatgaagaa taatcatata cgtcatggtt caatttattc attgcaacta
     3481 agtcatcgtt gcctttttct ttgaatacat agtctaccat attgaaatct ggataagtta
     3541 atggatgaac ttcgatttct ttgtctccta ctttaaatga caagtcattc aagaagttgt
     3601 agaaacgatg tgcaccttcg attgatgcac ccattaattt accatatcct gtgacattca
     3661 aaggtaagac atggtgtgct gcccaaacgc tggctgctgt tgcccctgct tttgaacctt
     3721 ccaagatata agccccaagt aatgctggga tatctgcgcc tttttcaaat acatatgtcg
     3781 caaagtaaga gataacgtct ctcatgcgga tatctttgat gacgatacca ccggcagaat
     3841 atggaacata gcccatttta tgtgggtcaa tcgttacaga ttctgcttct tcgattgctt
     3901 tatacgcact gtgtacttct tctaagatat aatctttgtt ttctgtaaag acattgtatt
     3961 tgtaatgaac atcttttaaa tcttcaaatg gaatgaagtt gttgtcttcg tctaagaaaa
     4021 tcgcacggcc gtatccgcca taagcagcat ctacgtgtaa gtagaagtaa ataccgtctt
     4081 tttctaatac gcgacgcaat gcaacgattt tatcgatacc gtcgattgct ccttcttctg
     4141 ttgatcctac aacaccgact acacctagga ttggtgtttt ttcagctgct agaccacgaa
     4201 cgattttttc aagttcgttg atgtccatgc gatagttgtg gtcaactggc actggaatca
     4261 cttggtctaa accgataccg atgatgtcgg cagcttttag ccatgaatag tgtttagttt
     4321 gtggaaccaa ccattttcct aatttttcta aatgttttcc gctacgtgct gagtgagctt
     4381 tgatttcgtc gattttttct gggacgctgt ctaacagatt catgatttct tctgttgaca
     4441 agttcattaa ttcccaatcg ctttttccag caactagttc aggtgtcact tctttcattg
     4501 caagcggcaa tgatttgatg ttgcgggcat accaaagtcc ttctaagttt gctaaagaac
     4561 catcagcaac gatgtggccc cagccatctt tatagctcat taattttgca aattccattc
     4621 ctacttcttc ttccatttgg cttgttgcag gagaagattc ataggcaacg ttattaccat
     4681 tccatagcat agcaaaattg tacgctagta aagatggcat caatgtttca gagttcatat
     4741 gaccccagta acgtccggca ttatgccaag gaacggaatg ggtacgcatc cgcgctgaaa
     4801 tttctgatag aacatccttt gttttattca cagtgtgttc aaaactagca ctgctttttt
     4861 cttctggcgt aataataggc atgtcttgag gcatgtagtt ttgacgccag cctaaatgtt
     4921 catccaccaa ttcattcaac aaagctttat agatttggcc gttctcagct ttgtccccaa
     4981 tgaatagggc atttaggttt agatctttcg acaatgattc actcatagtg aaaccctcct
     5041 aaaaaaatat ttaaattttg tcgagcccta cattacacct gttcttctat tctgtaaact
     5101 aagttttggc ctgattttta atcatatttt aatatgtatc aattatccga attgttcaca
     5161 cttttgaaat aattaaaaaa aacgagggaa tttacaaatg taaactccct cgcaaagatt
     5221 gtattaacga caaccagcgt tctattttag gatgggtttc aaaattggaa tgttgtaata
     5281 gtatttccct ttttcaaggt aaaaacttac taaattctaa tcaattactt tggccaaaaa
     5341 gtaatttttc ttaccttttc gaataaccac aaacttacca tcgaattcat cggatggatt
     5401 tatgacaaaa tttaaatcgg taacgcggtc accgttaata cttatagctc cgtttgaaac
     5461 atcttcacgt gcttgacgtt tagatggttc aatttttgtg gaaacaagca gttccacgat
     5521 attttcaggt gtgcttgaaa tttcaacgtt cggcatttta ccgaatcctt gcgcgatttc
     5581 ttccgcattt agttctttaa tatttccaga gaacaatgct tgcgttattt tttgcgcttc
     5641 tttcaagtct tcttcactat gcacaaatct tgtaacttct tctgccaaac ggcgttgtgc
     5701 ttcacgtttc tcaggttctg tctctacttt tttcgctaag tcttcgattt cttcttgaga
     5761 caagaaagta aagaatttca agtatttcac tacatcgcga tcatcttgat tcaaccagaa
     5821 ttggtagaac tcaaatggtg aagttttctt cggatcaagc caaacggcac cgcctgcagt
     5881 ttttccgaat ttcgttccgt cagccttcag catcaatggg atcgttaaac cgaacgcttc
     5941 tgcttctggt ccttctaatt ttcggattag gtctaatcca gctgtgatat ttccccattg
     6001 gtctgctcca ccgatttgca attgcacatc gtatgtttta tgcagatgca aaaagtcgat
     6061 agattgcagg atttggtaag taaactctgt aaatgagatt cctacttcca aacggctggc
     6121 aacgatatct tttgctaaca tcgtgttgat gttgaagttt ttaccgtagt ctctcaaaaa
     6181 ttcaagtaaa gagatttttg acaaccagtc gtagttgttc acaaatgtga tatttgcatc
     6241 tttaccaaac aattttctca tttgattgga aagtgcgtcc acgttatgtt gcacttgttc
     6301 catcgtttgc aacacacgtt ctgttttgcg gccgcttggg tcgccgatcg ttcctgttcc
     6361 gccgccaatc agaatatatg gatgatggcc tgccaattgg aatcttttca tcatcataaa
     6421 tggaatcaaa tgtccgatat gcatcgaatc accagttgga tcaacgccgc aataaagtga
     6481 aatgctcttt tcttcaacga gctcgcgaag tctttcttcg tttgtttgct gattaatggc
     6541 gtcgcgccaa gttaattcat caataatatt cattttactg acctccgtgt agttttttta
     6601 tgaaagcagg aacctgctag catgtgtaat taattatttc ttcaggatag gataggaaat
     6661 aaaaaaagtc ccttggcata taagccaagg gacgaaatct cgcggtacca cccaatttgt
     6721 atgtctagat atccaacata cctctcgaac atttaacgct gtcgcgtggc aatcactgcc
     6781 aaagactcct gaaatgtaat tcgctttaaa gcatgtactg gtttgcagcg accaccagct
     6841 ttctgaaaca ttggctttaa caactacttc gtttcgatct tcgtcttgtt attattaata
     6901 tgtgtctatc atagagcgca actttttctt tgtcaacaga aaaaggatat tttttttaaa
     6961 ttcctcattt tttctttttt gaatccgctc taattccgaa cattttattt tcaattattg
     7021 ttaaaagaaa taactttttc tgtatttttt ctgcaattta tcgagaagtc actttacttt
     7081 gcctcttatt ctttcttcgt tacaataagg aatgggacaa gtaaaaaaat gaaaaagcaa
     7141 gggacgaggt atatggaaaa aaagcaaaaa attattttag gagccacttc tgctttaact
     7201 atcgttattt taattctaag tttgtttttt gtcatccgat catctacaaa aaaagaacca
     7261 atcattcctg aaaaaaaaat aacagaaaaa gcaaaagaac cgacagatga taagcaacaa
     7321 ctagttactc aagcaaaaaa tctggctcaa agctacgatt atgaccaagc gattgcgtta
     7381 ttggaaaaag atagtaagaa cggatctact gaaacacaac agcttcttca aacactaaaa
     7441 aaggagaaag aacaactgat cgactggtct gatcctactc agatctctca tgtctttttc
     7501 cacagcttga tcgtggaccc gcaaaaagcc ttccactctc aacaggcaca aggatacaaa
     7561 gattatatgg tgactgttga agaattcaac cgttcgatcg aacaacttta tcaaaatgat
     7621 tttgttttag ttaatttgaa tgatttgatc caaaaagacg aacaaggaaa tctttccttt
     7681 aacgggcttg cgctacctga aggaaaaaaa ccattggttc tttcacaaga tgatgtcagc
     7741 tattatgaat atatggataa ttccggtttt cctagccgat tggtcgtatc aaataacaat
     7801 gaaatcaaaa atgtctataa agagaaagat aaagaagaaa tcggtgactt tgacatggtt
     7861 cctctgatcg atgcctttgt caagaaacat cctgattttt cttatcgagg cgcaaaaggt
     7921 accttggccc tgacaggtta caacggagtt ttaggttatc gaacatcaaa atctcaatat
     7981 ggagacaatg aaaaaacaaa aaaagagatt aaagaagcta aaaaagtggc ggaacagttg
     8041 aagaaagacg gctggagctt tgcttcccat acatggggtc acttgaatat gactcaagct
     8101 tcactagaag atgtaaaaaa agacaatgaa ttgtggcagc aagaagttgc acctatccta
     8161 ggaaaaacaa atattctgat ttatccattt ggtgcagaca tcagcgactg gcagccttat
     8221 tcagcagaaa atcaaaaatt tgcttacttg aagagtcaag gtttcgatat tttctgtaat
     8281 gtcgatgctt ctacaccagc ttggggacaa ttaggtacgg atttctatcg aaatgctcgg
     8341 atcaatatcg atggcattcg ttttcaatct gatttagacg gaaaaaatcc tattcttgat
     8401 ccattcatca atgtccatga tgtctatgac aaagaagcac gcgaatctgc ttgattttac
     8461 gtcatttatg aaccaaaaaa gccgaactga cattttatcg tcagtttcgg cttttttatg
     8521 ctttatttct tcttcggtgc gattcccttg atcaggactt ctgtccattc tgctgtatat
     8581 ggaagcagtt ttgcgtatat ctccggctta gaaattcctt ctaccattgc ttgaaaatag
     8641 aggatcaaga actcatccga atatttcaaa tccacttttc cttctttccg ccctcgatgg
     8701 aaaagatcaa gcatgatact gaaactttct tgcgtatacg catccatcgc gtcagaaaat
     8761 tctttttttt ctttttttgt atagtatgtc atcaaatcga tataaaattg tttgttcact
     8821 cgattgagat aacggatttt gttttcactc atggcaatca gtgtttcttc aaatggaaga
     8881 ttttccgcaa tcaacgcttt cgcttcttgc cctaaatcat tcataaatgc aaaaaatact
     8941 tcataaatca gcccttcctt gctgccaaaa tatttaaaaa tcgtcgcttt acttacctga
     9001 gcgttctcag ctaccttatc cattgtcact ccagcggttc ctatgtctgt gttcatcaag
     9061 tcaaaagctg cttgtaaaat ctctttcttt ttctcttgtg ctctcttttc aaaaccgttc
     9121 atttgtagag cctcccgtaa ttttaaactt ttataatata tatagttcaa atttattctg
     9181 aggtttatta taactatcta ttgagaaagt atcaagctag caattccttg tttatgaatt
     9241 cattgacaga aatttataaa aagaatatta tgtactaaaa taataaaaaa agttttataa
     9301 aaaggtataa aaccttttta ttaaactatt taatcttgtt tagtatatat ttttagaaaa
     9361 aggagtgggg tttttatggc agaaatcgtc aaagtcacac atttgaaaaa gagctttggt
     9421 cgtttcgaag ctttaaaaga tgtctctttc accgtacatt caggtgaagt cgttggattt
     9481 atcggaccaa atggttctgg aaaatccacg acgattcgga cacttttagg gattataaaa
     9541 aaagacggcg gaacggcaac gatttttggg caagatgtat gggaagatag tttatctatc
     9601 catgaacgtc tttcttatgt tccaggcgat atctctttgt ggggaaattt gactggagga
     9661 gcgatcatcg atctcttcat gaagcttcat ggaaaaggga gtgctcaaaa aaaagatgaa
     9721 ctgattcaac gtttctctct tgatcctaag aaaaaagcaa aaaactattc aaaaggaaat
     9781 cgccaaaaag tgggcttgat tgctgctttg gctgttgatt cggatctcta tttatttgat
     9841 gagcctactt ctggtttaga tcctttgatg gaaacgattt tccaagaaga agttgaaaaa
     9901 ttgaaagaac agggcaaatc catcctactt tcttctcata ttctcagtga agtcgagcga
     9961 ctagcggata aagtcgtgat catacgcgaa ggaacactcg atgaagaagg aacactcgat
    10021 gaattgcgtc atttgactcg ttcgaccatc actatagaaa cagaagcaga gaatagtcac
    10081 ttagcacaat tgaacggtgt ccatgacttt gtgcaaaaag gtcacctcgc tcaatttgct
    10141 gctgataacg aagctttcga tgtgatttta gctgaagcgt ccaaattagg tgtgaaaaaa
    10201 tttgaatcta ttccaccgac tcttgaggat ttattcatga gacattatga aggatgagga
    10261 ggaagcgaaa tgactgataa attttcacgc tggtccaccc ttttttctca aaaccttagc
    10321 cgtgactgga aaaagatcct gatatggcta gtcggtgtca ctgctttttc tggtggtttt
    10381 gtccctgcgt ttgaagaatt gacttccgga caaggattag ctggattata cgaaaccttg
    10441 caaaacccgg caatgattgc aatggtcgga ccaactccag cttcgaatgc tgctgagtat
    10501 actttaggag cgatgtatgc aaatgaaatg ttgctttttt gcagtttgtt tgctgctgtg
    10561 atctctgttt tacacgtggt gagccaaacg agaaaaaaag aagaacttgg tctgattgaa
    10621 cttattcgtt cctttcgagt cggacgtcaa gcaaactctt tagctgtcat cgctgaaagt
    10681 attctgatca atttgctttt agcaatatcg atcagctgta cgatgatttt tttccaagcg
    10741 gattcgatca ctgcagaagg tgcttggctt tttgggctat ctgtaggtag tatggggatt
    10801 ttggggagtg ccgttgcact gtttgctgca caactgatgc caagttcagc cagttcgaat
    10861 ggtctgtctt tagcgttttt aggtggactt taccttctac gagcaataac agatgtagct
    10921 gattcctccc tttcgatgtg gaatccttta ggctggtctt atttgaccta tccatttaca
    10981 actaacaact ggattccttt gctttatttg tttttgttca gtctaatctt gataatcagt
    11041 tcatttgttt tagaggaaca tcgagatttg aacaccggct atatccccga acgtgaagga
    11101 aaaccgtatg ctaaacgttc tcttttatcc ttgcgtggtt tattattcta tctaaataaa
    11161 gggatgacaa tcagttggct tatcactttt ggtttgatgg gggcggcata cggctctatc
    11221 tacgggaata tgcaacagtt tttagaaagt aacgacttaa tgaaacagat gtttatccaa
    11281 tctggcgtct caattgaaaa aaactttaca agcacgatca tggtcgtctt gatttgtcta
    11341 gctgctattt taccaattgc tagcatcaat cgtttattta cagaagaaac ccacggacgt
    11401 atgaatcagc tgttcgcaac aaaaatttct aggaaccaac tttattggac caatatgtta
    11461 ctagctattc tatttgctat attggcaatc ctgattagtt ctggtgcact agggctttct
    11521 gctttggctt cgatgaagga tgcgaaagac atggatctat tgatgtttct taaagccggg
    11581 ttcaatttcc tgcctgccgt tttagtgttt atcggaatta gtggctttct attcggatgg
    11641 cttcctcgaa tcggtaaact tgcttatatc tacttagctt actcctttgc actcaattac
    11701 tttggcggaa ttttggattt accagaatgg ttttccaaaa cagctgtccc tagctgggtt
    11761 ccacggatgc caaacgattc ttttgatctt tccgtgttcc ttacgttatc atgtatcagc
    11821 cttgcactgt tcgtccttgg ttctattggt tataggcgaa gagacttact agaaggataa
    11881 taaaaaagca tgtacaattt catgctagcc tccaaatctg ggtcaaaaat ccaacatttg
    11941 gaggtcattt ttttatttac aagtcttcta actgctcttt atcaatgatc cagacttctc
    12001 gattctttct ttttacataa ccattctcct ctaataattt aaattttcta gataaagttt
    12061 cgggggtcgt accgataaat gcagcaagtt ctttcatttt cattggcagg aaaaagtgat
    12121 ccttccctgt cgctttagct aagtcaagca gatagacagc gactcgttct tctacttttt
    12181 ccatcatcaa aaaagttgtc tgctgctgta cttttgttaa tttctcaacg ttgattgcta
    12241 aaagtttcag actaagggcg ggataatgca gtaagagttc cgagaaatct ttttgtctca
    12301 atatgcaaat ttccgttttt tctagtgctt cgccaaataa agactcattt ggaacgccta
    12361 gcaactggct ctctccctca tatccgcccg gctctattac tcgtaaaagc tgttcctttc
    12421 cgctagcaga taattgatac actttcatac tgccggaggc cacgatcact aattgaggtt
    12481 ctccattcgg cgcaagaatc tgttctcctt tttccacata gtgatgtttg acaagtgcgt
    12541 gtactttttc ttgatcttgc tgtactaagt gattgaacaa aggaacaaat gaaacacata
    12601 aatgatcatg actcataatc tctcctcctt ctaaacagct atttctaata aagaaacata
    12661 acttccctta ttccaactga taattttctt ttatcatagc ataaacgtca agcgaaccag
    12721 ataccagcaa atagagtgga tttctttttg tcccttgctt tattttcctt cttttgagac
    12781 cttaagtaat tgattcctat caatttttta aatatttact ttttgaataa tcaaaaacaa
    12841 accttcactc gcaagccgag agtaaggttt gtttttcgca ttgcttattt cgtgttgtct
    12901 attgaaattt ttccgcacct agcttttgat atagtggtgc tagttcttgt aaggtttcag
    12961 cggttatttg ctcaacgtgt tcgatttctt gcggatagaa gaatagttta cccatttgaa
    13021 aggttcggtc tctcatcgct tttttcaact cttcttgttt ctttgtatcg gacaaatagt
    13081 cagacaaagc taagtggtcg tcaagttcat gttctggttg cggccatatg taataatcct
    13141 tgatatcgac tgttttgctc cactctttca gtatcgcaag caactggtta tacgctgcaa
    13201 tgctaccata tcgttcttcg cttttataat gttggtacat caaatagatt tggtattgtt
    13261 tttgattcaa caaaactgct agacaggcat tctcgtcttg acgagattcg cttcggtacg
    13321 cgctccaaaa atgactgcgt aaattccagc cattcgtcca gctttcgatt ttaggtttgc
    13381 tcatgccgta catatcaggc aaaagtgcag ctgtctggag ctgaatctct ttccattttt
    13441 gccagtgttc tttatattct ttcttgattt gttcaatctt atctgttgga tggctctctt
    13501 tgagctgttt gaatgcgtag attggtcgat caaacaactg ataatctgat tttccaagtt
    13561 ccattgtttc gcctctttcc gttatctttt gtattctacc tgaattttgc gatatggaca
    13621 aatagaggtt gtgaaaaagc tgttctgcat caagtcataa gaacagccaa acaaaaaact
    13681 gcgatgagca tcaccgcagt tttattgaac ttaaggttga ttgatcacat ctaaaagcgt
    13741 tgaaaataaa atggcactag aagcagctcc tgggtcgata tgtccgattg cacgctctcc
    13801 tagataggaa gctcgtcctt ttttagctac caagactttg gtgtgttctt ttgcctggtc
    13861 aatgatttct tttgttaaat tccctgcctt caatgcctct gtgactggca gccaaacatc
    13921 taacatcgtt ttatcgtcag cttctgcctt tcctcggctt tgaatacccg ctgttccctg
    13981 ctcgatcact tcgcctaatt tttcttgaga atcaatagaa tcgacctctt ttgtcgcttt
    14041 catcatgttc atgaatgcag atccgtataa cggaccagaa gccccaccga ctttcgatat
    14101 catcgccata gagaatactt tgaatgtatc gctgattgtt tcaggatgat cggcttgaaa
    14161 agcttcttca taagctgaaa cccctcttcc catgttattt ccatggtctc catcgccaat
    14221 cggggtatct agatcactga gatattgttt gttctgattg attgcctctg caaattgcgt
    14281 gagccattct tggatttctt gaacggttag tttcatatta tcaccctttt atcttgagat
    14341 tttttaccat gaaatcgtct gtacattgct ttctaaagaa tctttccatt gtgtctccgt
    14401 caagtcgatc agtgttagag aaagaccttg catatctagt gaggtcataa aattaccgac
    14461 ttttcggaaa actacattga cgcctctttc ttccaacaga gctaaaacat cattcatgaa
    14521 cacgaactgt tccatcaaag gcgtcccgcc cattccgttg actagtacac ctgcttcttt
    14581 tggtaaattc gagtaatcat ctaaaatctt agtaacaagt tcctttgcca gtactttaga
    14641 aggctgcatc ttttctcgac gatagcctgg ctcaccgtgg ataccaacac catactcgat
    14701 ctcatcttct cctagttcga atcctggttt ccctacttct ggtacagttg ctgctcgaag
    14761 cgctagaccg atcgtcttag ttgcctgtac gaccttttct cccagttctt tcaactcagc
    14821 tagggaagca ccgtttcttg ctgcatcgcc cacgattttg tgtacaagga cagtacctgc
    14881 aactccgcgt tttccagcag tatacgtact attttcaacg gcgatatcat catctacgat
    14941 cacactttct acttcaatat cgtccatagc agctaattcc tttgccatat cgaagttcaa
    15001 tgcatcccct gtataattct taacgatcag caaaactcct tttccactat cggcttcctt
    15061 aatagccgtc tgaatctgat ctggagttgg agaagtaaac acatcaccaa gcaccgctgc
    15121 acttaacatc ccttccccta caaacccggc atgggatggt tcatgtccgc ttcctccacc
    15181 agaaaccaaa ccaacttgtt ccgctttctg attcttggct acaacacggg aagattctac
    15241 acgatgaaca agttctggat agctttttac taatccttcc agcatttctt cgacaatatt
    15301 gcttggatca ttcatgattt ttttcaattt attcccatcc tttctttccg gcattttgtg
    15361 cttctgttaa gatttgctga gtatcatctg ttataccggc agtgatggct gctgcaaaag
    15421 ctccttccac taaaggcgca tccaccagat gataacgttg ctgctgctct tcttccaaca
    15481 tgtcttttgc cagttccgag ttcaataccg cgctaccgat atccgcaaaa acaaaataac
    15541 tagctgcatc gcttgattca ttgactgctt ctacgatttt catcgggtct gatccgatct
    15601 cgccatttat tccgccgaga gaaaaaattt gcacctcttc ggatttttgc atctgctcaa
    15661 tcatttcttt aataccatct gttacttttt gactatgaga aataagcaaa atactttttt
    15721 tcacaagaat cttcctttct tttacatagt tgctaacgat tggtttcgct ttcattaatg
    15781 aacaattcaa gtatataata aatttttcgt ttgtcaaatt aagctagaca aatcatcttt
    15841 acctacgtaa ctcaaaaata cttccaatgg tgtgcgatag tgtaatgatt ttctaggaat
    15901 atgatttcgt tttgatgcca ccgattggat aaaggtttcg gataccttat taaaatccat
    15961 agctttatat aagccatctt tacgtaataa gccattcgaa tgttcattta gtcctcgttg
    16021 agaaggtgtt cctggatcag cgaaataaat cgaaatatca ttggtattac taatcttttt
    16081 ccaattcgag aactcttttc cacaatcaaa tgtgatggat ttaaacaagt gtttcggtac
    16141 agattgaaac cattgattca gtctattctc aatatctata gcttgcctac cagcgggttt
    16201 caaggtgatg atcactttag ataatcgctc aactaacgtg atcaccgcac ttttatgttt
    16261 tccaccgaca atggtgtcac cttctaggtg accaaactca tgattgaact gtatataatc
    16321 agttgttcta tcctgaatcg aacggcgaaa agcttgtttt cctctttttt ctttatgtcc
    16381 gtttggtttt cttttccctt tcattggtaa atcgtgaggg tcaaaggttt tatctttaaa
    16441 catccgatat agtgtacgta ccgaacaagc aataggaaac tctgctcgac caataaggac
    16501 atctggagtc cacccttgag ccaccttttt ttgaatgtat tcttgttgcc catctggtaa
    16561 cacaatcgga cgtcttccgc agttacgttt gtttttctta tattgttcat agtagcttat
    16621 tgcgcttcca ccagatttaa gaaaacgata gacattataa atggtttgtc gagaacgttg
    16681 taccagatgg gctacttttg aaacagagtg tgattgattg aaataagctt ctatcattac
    16741 gagttcagtt ggtgtaagat gggtataggt catttgtact cactccttat aattctttcg
    16801 acggaactat tttgagtgta gcacaaatga cttttttagt tgtctagctt aattttacaa
    16861 tcggcgattt ctaattttgg gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta
    16921 attaaaagag aggagtgatc gaatgattac aacaacaacg aacagtgtag agggaaaagc
    16981 cgtagtagct tataaaggga tcgtttttgg tgaggtcatt actgggatcc atgcatttaa
    17041 agacttggag gcggggttgc gaaatatctt tggcggccgc tcaaaaagct atgaaaacga
    17101 actaatgggt gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg
    17161 agctgatgcg atcattggcg taaaaatgga ctatgaagta ttaggcagcg acaatggcat
    17221 gctgatggtc acatgtagtg gaacagctgt ctccacgaga taagtagggg acatctgttt
    17281 taataactaa agagagatgg gacatgtagt ggcactcaag aattagattt ttcagtctaa
    17341 cttcgaggta ctctaccttc ccatcctctc ttttttattt ctttttgcgg atgattccgt
    17401 aagctgctgc gactaacaca cccgcccctg ctaaggaata actgacagaa gctgtttttt
    17461 catttgtttt tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt tgctgaactg
    17521 tttctgtcgt gcttccagag gtcactgatt tttctcctgc cgaatgcaaa atatctttta
    17581 ttcgttggaa actagctgct aagtcagctt tttttgcttc aaaatcatta gcaactgttt
    17641 gtgcttcgct gatctttccg tggattgctt gactggccgc tgtgatatcc gcttgagttt
    17701 gacttagctt tccagaagct gtttgagcaa cctgatttaa ttgctgattg aattcttttg
    17761 cttgttggat cttcccgtca agatctgttt ggacagcttc cgcttttgct ttcgcttcgg
    17821 caagcttttt ctcaagttgt gcacggtcat ttcccacttt atttttaaga tcagctaaat
    17881 cttgatcgat tgctgcttgg tcttctttca atttgttttt tgcagcttct aatgcttctt
    17941 cgagttcttt tcttttattt tccaagtctt gcaaatgatc gatttggtca gaagaggtag
    18001 aactatttat tgatgtatct tctgaagatg aaatcgtttc tcctgaatta tctgtcggta
    18061 cattggaagt catttctgtt tccgaggcac cgttttcagc accagaagag cttatggttg
    18121 ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa agctgtctgt cccaataaga
    18181 aaatactgga aacagcaatt ggccagatta acggcttcat tctttttttc atcacttttc
    18241 ccatccttac tactttttaa ctaatttctt attataagca aaaatgtttg gaagaaccat
    18301 aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg
    18361 gcttttccat gtcccgcttc cataacctaa aatcgttttg actgctggaa tgaaagtcgt
    18421 cacgatcgtt atcgcgttca ctatccagaa caaaagcata tatagtggtg cgaaaagaag
    18481 gtatttcaat tttcttcgtc gatcatctac aatcagcgaa gctagcaact ggaaaaatcc
    18541 tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta aaagtaatat aaatctgttc
    18601 gtagtttcct tgatatacat aatgcccgat taatccagca aaaagaacta cggaaataca
    18661 aaagaatatt gaccagacaa tactcaatgt ttgatcaatg aagatgatcg ttcttccaat
    18721 atgtttaaaa ggatggagca tcactttttt gaaattcgtc agccagactt ctgttccacc
    18781 ttttgcccaa cgttttctgt gtcgatatag catcttcaac gtcacaggta cttccatgaa
    18841 aaagataatc tcaggagcaa aaacagataa ccagtcattt aactgatgat cccaagcaat
    18901 actgatgtct tctgtcgctc gatcttgtct gaatccgccc acatcgatca aggcttcttt
    18961 tcgatacatc gtatttgctc cactgtacgc ataaagacca ccgaaaacag ctgactgtgt
    19021 tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt tgagattttg caatcaattt
    19081 ggtacgattt tggacatcca tatttgcagt gaccgcagca atatgtcttg caccagggcg
    19141 aatgaaataa ttcacatacc taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt
    19201 gcttaaaatc aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc
    19261 tttatttttt tcgatccgaa ccacacgaag attagcgtat tttttcatca gtctagctaa
    19321 tatttctggt gtttggtccg ttgaaccatc gtctgtcact aatacctcat agttgtgata
    19381 attcaatttc gtcatcaaat actcgattgt atcttcgatc acaatttctt cattatgggc
    19441 tggcaccatg atcgtgatga acggttcgac actcaaggga acatctaccc attctttttg
    19501 cttatgtttg tataaaaaca cataacacca gacaccaata aaccatacaa atccgcctaa
    19561 aatcggataa gaaacgagaa aaaaatacat aatagacaaa caagtatcta agaataaatt
    19621 acctgtatgg gtgattgaga tcatttcagt tccaccccat tttctttata caaatctgca
    19681 atgaatgtcg tatctagatt tttctcttcg gagacagaat aaaaacggac cgtttctctc
    19741 tcttcttttg ttccaaaacg ttccgtatac acttcttcaa gtaattcttg tctttttttc
    19801 aatttttcat cttc
//
LOCUS       DAFEHS010000051        19328 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFEHS010000051
VERSION     DAFEHS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.052"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..450)
                     /locus_tag="LP327_001862"
     CDS             complement(<1..450)
                     /locus_tag="LP327_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL1725972.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
     gene            complement(555..1418)
                     /locus_tag="LP327_001863"
     CDS             complement(555..1418)
                     /locus_tag="LP327_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL1725973.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1614..3713)
                     /locus_tag="LP327_001864"
     CDS             complement(1614..3713)
                     /locus_tag="LP327_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL1725974.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3933..5042)
                     /gene="rpoD"
                     /locus_tag="LP327_001865"
     CDS             complement(3933..5042)
                     /gene="rpoD"
                     /locus_tag="LP327_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBL1725975.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5066..6907)
                     /locus_tag="LP327_001866"
     CDS             complement(5066..6907)
                     /locus_tag="LP327_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL1725976.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7089..9404)
                     /locus_tag="LP327_001867"
     CDS             complement(7089..9404)
                     /locus_tag="LP327_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL1725977.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDHKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9589..10449)
                     /locus_tag="LP327_001868"
     CDS             complement(9589..10449)
                     /locus_tag="LP327_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBL1725978.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10508..11029)
                     /locus_tag="LP327_001869"
     CDS             complement(10508..11029)
                     /locus_tag="LP327_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBL1725979.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11307..12068
                     /locus_tag="LP327_001870"
     CDS             11307..12068
                     /locus_tag="LP327_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL1725980.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12094..14685
                     /gene="mprF"
                     /locus_tag="LP327_001871"
     CDS             12094..14685
                     /gene="mprF"
                     /locus_tag="LP327_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL1725981.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14873..16060)
                     /gene="tuf"
                     /locus_tag="LP327_001872"
     CDS             complement(14873..16060)
                     /gene="tuf"
                     /locus_tag="LP327_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL1725982.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16428..16784
                     /locus_tag="LP327_001873"
     CDS             16428..16784
                     /locus_tag="LP327_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL1725983.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17025..18029)
                     /locus_tag="LP327_001874"
     CDS             complement(17025..18029)
                     /locus_tag="LP327_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBL1725984.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18195..19046
                     /locus_tag="LP327_001875"
     CDS             18195..19046
                     /locus_tag="LP327_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL1725985.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19063..19308
                     /locus_tag="LP327_001876"
     CDS             19063..19308
                     /locus_tag="LP327_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725986.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         5392 a         3990 c         3383 g         6563 t
ORIGIN      
        1 tttttcatag cattcctgac agattccatg gaacgttagc cgatgatctt ttactaaaaa
       61 atgataacgt tcttctacta cttgctccac ttcaagtaaa agatcctctt ctacttcctc
      121 gatcgtgccg cagttcaagc atagtaaatg atgatggaaa tgtttggcgc cttcttttct
      181 caaatcataa cgggctaatc catcattaaa acttacttta tcgacaattt tcaattctgt
      241 caagatttca agggttcgat aaacagtagc aagtccaatt tccggactct tttttttcac
      301 taaaaagaag atttcttctg cagataaatg ctctttctca ttttccaaaa gcacaagcag
      361 agtagcttct ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct gttttgtttt
      421 tttcatcgcc gaaatagcat tcatgaccat ttactcaacc ccaacaattt ataataatta
      481 caaataaaaa aagatataac actatgttaa ttaaaattat tataagctag ttaagaaaaa
      541 catgcaaggg attctcaatt agattttttg actagttttg tcacaccgtt ttcttgtgta
      601 atcaatcctt gtttcaaaag attacctaat gcgcgcttaa atgctccttt gctgatacca
      661 aaagctttca tgatttcttc tggatccgat ttatctgtaa agttgatttg ctgatctgca
      721 gaacgttcta aaatggttaa aatcatttgt gcatcatctg atatagcttc atatgctctt
      781 ggcttcaatg aaagattcag tacaccgtct ggtcggacac caatcactcg accttttatc
      841 tgctcgccta gacgaggttc attatatcgt tcagaaggat ggataaaccc aagatagaaa
      901 tcttccgtta aaacgtacgt acctgttaat ttcaagcggt atgctgttcc tatcacatct
      961 ttgtttttca tttcttctgt tccatatttg cttaaagatt tgaatatttt ttcatctgct
     1021 aaactgcccc aaatacggtc ttttgcatca actctaaggg aaatcatcaa gcgatcccct
     1081 tttttaggcc atagctctgt cattgtcggc aattcatcaa gagatacgac aaagtcttta
     1141 tctggaagac cgatatcgac aaacgcacct aaatcttttc gtgtagcagt aacgattcca
     1201 aaagcgtaat gaccgatacg gcttttagga atttgtgtag tgatccgatt ttcatgtttt
     1261 tgattttggt aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc ttctgatttg
     1321 cttaagccaa aggtttgtcc gtttttttgg acaaagtagt ttttttcatt ttcatcaata
     1381 ataatacctg taaatatagt tgctaataaa tcgttcattt ttttctccct ctatagtgaa
     1441 aggttttctt aatacatttc tttgctatca taacataatg ttatctactt gaatagctga
     1501 gccttggttt ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata ctcccctttt
     1561 tcgaataaac ggtgttaaac ggcaggctca cagcctcttt catttgccta aaattaattg
     1621 ctcccatttc ctccgaacca tccagtttta cagttgatgg ttacgatgta aaatggcaaa
     1681 gcactttctc caatgccttt catcaatgca gattttgttg aagtggcata tagattataa
     1741 tagccttccc cattttcgat tctggctttt tctaatctgt aacctccttc aacgatatat
     1801 ccacgttgga tacatgtagc aatcactttg ctttggacac ctggtgccca cgtccatgct
     1861 ggattagatg catcggcaat atcacttgct ctggcagatt tgattggctg attcgctgag
     1921 tttgaagagg aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc gaccgcagct
     1981 tgttgagcgg cttgagctgc agctgctgct tgagcggctt gttttgcttg ttcttcagct
     2041 gcttttttcg cttcttctgc ttttttcttt tcctcttctt cttttttctc tttatcagtg
     2101 agcatcttat ctacttgttt caattgttca ttcaactgag cagatagatc tgtatttttc
     2161 actttttcta ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga atccactact
     2221 tttccgtctt taaagactat agctgttttt tctttagctg tttgcaattc agcatattga
     2281 tttttagctt ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt atccgtttcg
     2341 attgcttgga tctctttatc tgctttcaac ttgatgtcat ctgctagatg atcatcgacg
     2401 atggcaggtg ctatgaacca ctcattcact ttttggatat ctcggatctt agattggata
     2461 tcttggtaag ttgtttctaa ctctgaatat cctttttcat tcttaagact ggataaatca
     2521 ttttttagtt ttgataaatc ttgatgaatc atatctgcac gaataaattg atggtgatca
     2581 tctgtataaa aggcagcaag tttctcatga agttcttcca tttgttgctg tctttttgct
     2641 tctgctgcta cttcggcttc tactttttga tgatggtcat aataagccca gcttcctcca
     2701 ccgagtagaa gtactgctgc taaaacgaga taaggtactt tttttgattt tttcttttgc
     2761 gctttatctg gatctgtttt ttctaagttt gattcttgat gtgttttttc agtatctggc
     2821 acgattgtta ttgatttcgc ttctgcttca aacagcttag gctggttctc ttctttttct
     2881 atttttgctt caagatcagt accctctttg cttacagatt gattttcagc atcggattca
     2941 ctatttattt catcgggctc tgttgacttt ttgtctgtgt tttcgagtat ttcttttttc
     3001 tctgcttccg gcagtttatt cgttttttcc ttttctaatt gtttttcagt aaatacttca
     3061 gttttttctg tgatatccgt ttttacgtct tttattttta ctgtttcttt acctgtggcg
     3121 ttttctctat cagagtcttt ttcagtttct tgttcctttt ctggctctac tttgttttcc
     3181 tgtgcaattt tttcactgat cattttgagc agctctttcg cgtgctcttc ttttgtatct
     3241 tcacgatgtg ctttgatata agcggatagt atcgaatttt cttcctcatt atcgacgatt
     3301 tctttcagtt cagctactct tcttttttgt tttgattcaa gcgtttcttc ttgattcttt
     3361 cctgattctt ccgaagtctc atgaacagtt gctttcttct gttcattttt ctctatatta
     3421 gttacttgat cgtgagcagg tggttcttcg tcttctggtt ctgctttttc gaataattcc
     3481 attacagatt ctagaggtaa atctttcaat tccgaccatt cgattggatc gtttaattct
     3541 gtatcaggaa agactgcatc ttcagttgat ttatcctgca tagctgcatc tgtatttgtt
     3601 tcttcaattg attcttttat ttgctgatct tctgcagtta tttgatgacc gcagtctgga
     3661 cagatagtat tctgctccat ttttttcgat ccgcaatttg gacatttttt catcacttag
     3721 cgatcggctc ctttactagg tttacgctac catttaatct atcatattct atacgtcacg
     3781 ccaatagctt atttttagga tttacttaaa aataatgaat gaatttcctt cctatagaaa
     3841 aaaaagcttg agactgcgta gtctcaagct ttttttcttt taaaataaac caaacagttt
     3901 tactgatttc tcttctggtc aatagtccac gcttattcta agaaatcttt taattgtttt
     3961 gaacgagaag gatggcgcaa ttttcttaat gctttagcct cgatctgacg aatacgttcc
     4021 cgagtaacac caaatacttt ccctacttct tccaatgttc tcgtccggcc atcatctaaa
     4081 ccaaaacgta agcgaaggac attctcttcc cgatcagtca atgtatctag tacatcttcc
     4141 aattgttctt ttagtaactc ataagctgcg tgttctgctg gacttgttgc ttcctggtct
     4201 tctatgaaat cacctaagtg agagtcatct tcttcaccga ttggcgtttc aagggaaaca
     4261 ggttcttgcg cgattttcaa gatttctcgg actttttctg ttggaagatc catttccgca
     4321 ccgatttctt ctggcgtagg ttctcttcca aggtcttgca gcaattgacg ttggatacgt
     4381 atcaatttat taattgtttc aaccatatga actggaatac ggatcgtacg tgcttggtca
     4441 gcgattgctc gagtgatggc ttgacgaatc caccatgtag cataggttga aaacttaaat
     4501 cctttgcggt agtcaaattt ttcaactgcc ttcatcagtc ccatgtttcc ttcttggatc
     4561 aagtcaagga actgcatgcc tcgtcctaca taacgtttag cgatacttac tactaaacga
     4621 aggtttgctt ctgccagacg ctgtttcgct tcttggtcgc cttcttcgat tttcaatgct
     4681 aacgctactt cttcttccgc agttaaaagg gagacacggc caatttcttt cagatacata
     4741 cgaaccgggt cattgatttt tacaccggtt ggcgcagaaa gatcttctgt ttttgcttgt
     4801 tcagcttttt tctcagcact cttcaagctg tgaattgatg gatcaccatt ttcatccacc
     4861 acactgatac ctgcgtcttc aatcttttgg atcaatttgt ccatttcatc tgcattcaaa
     4921 gtgaatggtg tagcaagctg attggataaa tcatcatata caacttgtcg tttgggtttg
     4981 ttttctttga tgaaagctgc tactgctgct tcatacgttt ttttatctgt tccatttgcc
     5041 ataaaagaag gccccccttg agtagctatt tggcttgttt taactgtttc gttaaattga
     5101 tgatttcaat cgtcaattca agctcaagct gtttgtttcc agttcgtctg gcttcttgct
     5161 gcatttgttt ttttaatgtt atttcatcag ctaagccaga tttgctgatc acatctagca
     5221 agtcttggat ttctcgtggc gaactttctt ctgccaccgg taaatattcg atttccacca
     5281 ccaaccgcct taaattttca tcttgcaaaa agtctaaaaa ctctgcaagg ttgaattcat
     5341 aatgttgtga gacatatgca tcgaataaaa aataaacttc tgcatatgca tcatgaataa
     5401 actgaaattc tcgttctttt aaaatattac ggacagaaac ttctttaaat gcacgatata
     5461 atagaatctg ctctgccttt tctacttgcg tcaaaggtct ctttttccgt gcagtttctt
     5521 gtctcgtaac ctgagtgtgt tgactttcct gaggttcttg acgattttgc tgatttgtcc
     5581 ttcgaagatc gcgcagttgt ttttgtatcg tctctctgcc gatctgaaac tctgttgaca
     5641 gttgagatag atacatatct tgttcgattg gtgaaacaac tcggctaagt tcaactagta
     5701 attcttcgag gtattcgatc ttgtcttttt cattatctaa atttttccct tgttttaaat
     5761 aacgtgattt aaaagtaaag actgtttcgc gcccatgagc cactaattct tggaaagatg
     5821 cttctccata cttacggaga tattcatcag gatcaagctt ttctggaacg actacgacag
     5881 aaagtgctaa tcgactgttt tgccgaagca attcaatccc tcgattggtt gcttcaaaac
     5941 ctgcgttgtc tccgtcatag caaaagacca attctttcgt cagcttttcc atttgttgga
     6001 tctgttgagc tgttagactt gtccccatag aagctaatcc agttttgata ccagcctgcc
     6061 atgcagcaat cacatccata aacccttcga acaacaaaat ttctccgttt ttccggatat
     6121 cgcttcttgc tttatcaaga ttaaataaaa cttgtctttt attgaacagt tctgtttctg
     6181 gactattcaa gtatttgggc tgatcttttt cctgcccttt ttcaggtgca agccagcgtc
     6241 ctgaaaaacc gatcgtctgg ccttgagaat tacgaagtgg aaacatcact ctttgataaa
     6301 aacgatcaga catacggcca tcatcatgcg tgacaaacaa tccagagcgt tctaacaatt
     6361 catttgaaat tgattcattc ttgaatactt gctgaagaaa agaacgctca ttaggtgcaa
     6421 aacctatgtt gaattcttca attaattcta aagacaaccc acgactttgc aaatattcta
     6481 aagctggctg tccagctttt gtatgcaata gcatgtgatg atagatttcc gctgcttttt
     6541 catgagcagc aatcagtttc cccgtagcag attcaggatt ttggacagct atagtacgct
     6601 gacgccattg atcttctaac ggtatctgtt cgaagtctgc cacttttacc actgcttctg
     6661 gaaaacttag accttctagt tcttgaagaa aagtaaacac gtttccgcct ttaccacaac
     6721 caaagcaatg aaaaatctgc ttgtcttctg caacagaaaa agacggtgta cgttcttcat
     6781 gaaaaggaca cagccccaag taatttttac cagacttttt caactggaca tattgtccaa
     6841 tcacctcaac gatattcgtt tgactgcgta ctgtttcgat aacctcttga ggaatccgtt
     6901 gagccatttt ttcaccccct gctctattga tatcttttgg gtttaacaga agatatttca
     6961 ggagaataac cggtaaaaaa tcgcactaac tgaaatcagt gcgattttaa aggacacaat
     7021 tatacatgtt tgattgtatc atatttcatt gctttttcaa gtataacaca tttctatctc
     7081 attaagcatc aatttgctac tcttttcagt aactgatttt gaatatcagc cttccagtac
     7141 aatactcgaa gaacaatatt tacgagcatg aatacaaaga agtatcctac gctctctccc
     7201 aaccctaagt ttacttccaa aattccatgg aacagcattg cagctacata tatccctacg
     7261 atcgtcgtta ctgctaggaa gatgcgcaat gggttgaagg gataacaagc ttttacaact
     7321 gccatgcaac tgatcccaat cagcaagtaa tacatcaaag tagtagtttc agcatttccc
     7381 catgcgaatg tttttccaag tagatacacc gcaatgatat tcactactac aagcaaggca
     7441 tttggtaaag cattaataag tgctgtaggc aaataacggt aagtgatttt tcttttatct
     7501 tgttcaaaag ataaaaagaa ggaaggatac ccttcaatcg ctaaatcaat cagcgtgatt
     7561 tggataggga taaacggaaa tccaattgct gtaacggcac atataatcga taaaataaat
     7621 gaataaatcg ttttgataaa aaagatgcct gagactttcg tcacattgtt gaccactcgg
     7681 cgtccttcaa acaggacttc tggcaatgta gtaaaatcag agtccaacaa taccagattc
     7741 gcaatctgac gtgttgctcc atcaccttct gccatagcaa tgctgcaatc tgcttctctt
     7801 aacgccaaaa catcattcac accgtcgcct gtcattgcaa ccgttctgcc gctttctttc
     7861 aattcattta ccagcaattt cttttgctgc ggactcactc gtccaaaaac cgtatagcga
     7921 tgagcagctt ctcgcacttc catttcttca gtaatttgag aaagatcgac atacgcatca
     7981 taattcggta atcctgcacg tctagcgata ttggagacag taaaaggatt gtctccagaa
     8041 atcactttca gatccacacc ttcctcatga agataagcta gtgtttcttg agcgtttttt
     8101 cggattggat catcgatttc aaacagagca accggctcaa caaatgctgg tttttcttcg
     8161 ttcaattgct gatttggagc gatccccaac ataagtacgc gatagccatt cgcttgggct
     8221 gtttttagac tgtccggaag ccgatcttct tccaccaaac gttcaggcgc tcctacgtac
     8281 acgactccca gttctggaaa ttccatcgcg ccccatttgt gatcagaaga aaatgcgact
     8341 ttgtttgtca catcataccg attagaaagc tcaaaataat ctctcatagc ccgcatagtg
     8401 atgttgttat cactgctttc tgccagatac gtccccataa tttctgggat ggctttctcg
     8461 tacagcggat gtagtgcttc tacccgctgg atactcattt tcccttcagt aatggtccct
     8521 gttttatcca agcataatgt atcgacatgg gccagcgttt caattgaata catgtcttga
     8581 acaaggattc gtttttttgc cagtttggtt acccctgtcg ttaatgcaat acttattagc
     8641 aataccaaac cctttggaag catgcctaac aaagctgctg cagatgcaat aacagaaatt
     8701 ttgatagaag catcgcggat gaacagtgct tccaaaaata gtatgatccc caaaggaata
     8761 atcacgaaac tagtgaattt ggatactttg cgaatcgaat ctacaagttc agaacgaata
     8821 gctttgtgag tctttgcctc gctagtcaag cggacagcat aattatctgc acctactcgg
     8881 ataacttcag ccatgacaga accgcttgtc aaaaagcttc cagaaagcaa ttcagctcca
     8941 gtttctttga caatcaaatc agattcgcct gtcaaaagag cttcatttgc ttctgcccgt
     9001 ccagatataa cctgtaaatc ggctggtatc tgttctcctg ctccgatttt taccaaatct
     9061 cctaatacca gctctttagt agaaatcgat tgttctttac cgtcccggat cactagtact
     9121 tctgattggg aaacaatcga taatttccgt acaagatttc ttgcatgtat ttcttgataa
     9181 attccaataa cgatattgac gacaataatt gctaaatacg ccatgttgga atacgcacct
     9241 acaaataaca gacaaatccc aataccaaag ttcagaaaat tgaataacgt caatatatta
     9301 tcgctgaata tttgttttgt actttttgat gcatcttcct ggtagtcatt ttgctggcca
     9361 gaagccattt tctgaactac ctcttgctgt gtaagtccat tcattttctt tttctccatt
     9421 ttccgccaac tttcaaaaat aatcacgtgt ttaagtctag cgtgttccaa gattaaaaac
     9481 aacttgtttt tataaaattt atagaaaaac acacgaatga ttttacaaaa aagatgccaa
     9541 cctttttgtt tgaatcaccc gtgtgctgtt tttattatca atctatcttt acttatattg
     9601 ttcctccaaa cggctcatcc accaacctag tgctgtaccg gctataaaaa gaacaggaca
     9661 agctagtacc ccagcaactc ccaatcccgc gtaatcggta ggaatgatca taatcgttga
     9721 agcaaaaact acacctaaaa tgaaatggaa tagcttgcta tatgcctttg agaaaatgta
     9781 atccatgatt tttgacaatc caagaacaca aagaatccca ccaattccga tcggcacaat
     9841 cacttcaagt cgtacttctt taaatccgtc agccatcgct ttatacaatc ccatatacac
     9901 caaaaaatta gaagggctca atcctgggac gatcataccc aaaccaatca atccgccggc
     9961 aatcagccat gttccaaaat tctgatcgac ttgcccgtca aataattgag cgccgtataa
    10021 taaaaacaaa taagccagaa caaaagtaac aaccatgatg atcacatcag aagtatctct
    10081 tccttttttc cctgcttctt tccataacgc aggaacagtt ccagcgatac atccgacaaa
    10141 aaaccataat atcgttgttt catacgcacc taataaaaaa ctgactaaaa aagaaagtag
    10201 aaagatgcct gtcactccgc ctagaccaac aggcagaaaa aacaaaacat tttctttgaa
    10261 attttttgta aggtgggcta aaaaagaaat cagccgctca tacatgccaa agacagctgc
    10321 taatgcacca ccgctgactc ctggcaagat aaatcctgaa ccgataaaca ttcccttcac
    10381 aaaccgtaac atccagtctt tggttgtgta ccccacttct tgattttcca ttttttgcct
    10441 tttttccact atttgcgcac tccttctttc atttaatttt attcatgatt gaacatttcc
    10501 tgctatttta cttaaagact ttttcatagg tctctattat tttttcattc attttgacaa
    10561 tatcaggata aaacatgtta cctccattat gatagagata gccgccattg taaagcaagg
    10621 actggatccg ccaatatcgg tattgtttcc cttcctcatt tcctaaaagt gggctcagca
    10681 catctttaga atacgcttct gctaattcta ctgtgttttc tccgccatga ttttttacat
    10741 aggcaatata atctttccca aaattatatg cttggacagc tgtccataaa tcgcatcctt
    10801 gtttctttgc ttctactatc atttccgcca aatatttgac accttgatca atactgtcag
    10861 ctggattatc aatgatctca gcttctccat gcagactttc cgagctctgc atgagatctg
    10921 tcccttttcc ttttgattcg gtcaatatta tcgccttgat caaattctcg tagttttcca
    10981 tatgataatt ttttgcagct gttgttattt ctatttgata gctttccact cgttttaccg
    11041 aacgataagt tcttcctcat aaaaacaaca ccgcaccaat aaccgtcaga atgatcatgt
    11101 aaaaaagaat acggataata gataatcgtt ttcgcttcat ttcatcacct agttattttg
    11161 acatcttttt ttatcttact atttctttat cttcttggct atttcttcct tgatttctgt
    11221 gtacgcctta agaaaaaatg aattttcctg tattttttga catcttacga aaatgatcgt
    11281 tgtttttcag taaaaacaat tgcccaatga ctgatataat cgttatactt tacacagtag
    11341 gtgattatgt gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc gaaatttact
    11401 atgaaaagag cggtcaaggc ttccctctct ttttactgca tggcaatgac ggcagcggcc
    11461 gctttttttc agaacaggtc cctgtactcg aacgctacta taccgtgtat ctagtagata
    11521 gccgaggaca tggtcgttcg acaaacgaag catctatgtt gaattttcag ttaatggccg
    11581 aggatctgaa tacgatcatg ttgctggaaa aaatcgatca ggcagatttt cttggattta
    11641 gtgatggagc aaatctagca ctggtatttg ccagcagctt ccctaaaaaa gttcatcgat
    11701 tgattttaaa ttctggaaac acgcttgtta aaggcgttcg tttttctgcc agagtcatca
    11761 gtaatattca ttatgcatgg gtatggttac tctctttatt tcgcccaagt cttcgcaaaa
    11821 atcttttagt tatcaaactt ttgctgcatg acatcgggct tacagaaaat gatttaaaaa
    11881 aaatcaactc ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag ctaaaacatt
    11941 ctttgtatat agctaaaact ataccaaaag cttcctttgt gcttgtgaaa gaacaaggac
    12001 atgaattagc tagaaaagat cctgaacgct tcaatcgaga agttcttcaa tttctttctg
    12061 aaacataact attaggaata gaaggtgatt tatttgttaa aaaataccat acaatggctg
    12121 aaaaaccacc taggactgtt caaaacaatc tttttgattt ctgtcattgt cattattgtc
    12181 ggtgaattga tgtccatcgg taaaactcta tctatccagc aattaggaga aacgtttgcc
    12241 actatacctg tatggaaaag cggactaatg ctattgattg gtcttatctc tgtgttacct
    12301 atggttggtt atgatattat tttgaatcgg atgcttgaac aaaagcagaa tcctcgttac
    12361 ctgtttgaga caagctggct gatcaatacg atcaacaata ttgccggttt cggcgggttt
    12421 gttagtgtgg ggcttcgctc tgaactatat ggacaaaaga aagagagcag aaacgtgatc
    12481 caagcattat cgaaagtttt cttgtttttg atggctggac tttctattta cagtctgctt
    12541 tcttttttac ttgtagtctt cacgccagtt gctccttttt tgaagcaata ttggatatgg
    12601 ttgatcggcg gcggtctata ttttcctgtc gtctttttat ttactacttt caaaaagaaa
    12661 ggattccttg gtggtacctc acttaaaact caaggacagc tattgctagt ttcattgttg
    12721 gaatggaccg gtgtcctgtt tagtttcctt tctgttggtt atttaatgga aatccggatt
    12781 gatctgtggc aaacaattcc attattcatt gctgcttcag tgataggaat cgtgtctatg
    12841 attccaggag aaattgggag tttcgatgta atgatgatta tcgggttgtc cgctattggt
    12901 gtgccaagag aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta ctacatcatt
    12961 ccgtttttga ttggaatcgt ctttttcttc aaaaatatcg gttccacttt tgatcagcgt
    13021 tattctggta tccctaaaca attagcaaca gaaattgcgc ataaaatcgt ggttgtctta
    13081 ttatactttt ctggaatcat gctagtactt tctgccacta tcccgcaagc ttttacagag
    13141 tttcgctggc ttcatagttt gaacccattg aaatttcatt ttattatcca atttcctagt
    13201 atactattgg gatttctttt aatcattatg ggcagaggga tcgcagcacg tgtgaaacga
    13261 gcatatttgc ctacaatctt cttgattgtt ctagctttac tttatgtttt attaagtgat
    13321 tttagtttca caccggtggt tttcttgtct attctgcttc tgatcattct tgcttctaaa
    13381 aacgaattat ttcgggaaca attgatctat tcatgggagt ggcgtacgat cgatggaatc
    13441 attattggtg cactgtcctt gctttacatc gttattgggg tatacaatct gccggatttc
    13501 cctcatcggc gtcaccattt tatttccttt ttcttatttc cttctgaaaa gatttggttt
    13561 tcgggattgt tggccatcat cgctgttagt ttcatgatcg ttctatttgt tcatttcctt
    13621 caaggagaaa agaaacagat tggcgaagcc tttaatgaag aaaaggccct gaagatcctt
    13681 acaacttatg gaggaaactc cgatagtcaa ttgatcttct taaaagataa gcggatgttt
    13741 gcctatgaaa aagacggtga accaactgtt cttctccaat tcgcttgctt caataataaa
    13801 tgtattgtaa tgggtgatcc ttctggtaag aaagaagatt ttccagaagc aatcgaagct
    13861 tttatcgaag aaacagatcg actatgctat ttaccagttt tctacgaaac aagcgaagag
    13921 attgttatga ttttgcatga attcggctac gatttcatta agatggggga agaagcttat
    13981 gtagacctga atagttttac aacttcaggg aaaaaaatga agggcactcg tgctgtcttg
    14041 aatcggatcg aacgagaagg atttacattt gatgtcttac aaccaccatt ttctactgag
    14101 caaatgagta ctttcaaaaa tatctctgat aattggttgg gttcacgaaa ggaaaaaggg
    14161 ttttctttag gctttttctc tgaagattat ctacaaagag cgcctattgc agttgtcaaa
    14221 aatacccaag aagaaattgt cgcttttgca acgatcatgc ctacttacac gaacaataaa
    14281 gtaggaacga ttgatctgat gcgatatgat tctaaaaaag caccttctgg cagtatggat
    14341 ttcttgttct tgcatttgtt tgcctatatg aaagaagaac atatccaatg gttcaattta
    14401 gggatggccc ctctttcaaa tgtcggaact tccagaaaga gttttttaca ggaaaggatt
    14461 gcatacttgg tttacgagtt tggttctcat ttttattctt tccatggatt aagagaatat
    14521 aaaagcaagt acgctacaaa ctgggtctca cgttatactc tatattctag agatagttgg
    14581 attgcatatg ttatgattgc tttgctgatt attgataatg cgccagttga gcagacacaa
    14641 ggtagaataa gtggtttgaa aaaatggata cgaagaaagt attaaaaact gcataccgat
    14701 agatgaacac tctcatgatg aataaaagag actgaagtaa aacgaaagtt catgcttcta
    14761 caatctcttc ttatacaatt gaagaacaaa gagcggtcca aacagaaatc ctgtttggac
    14821 cgctctttta ttttaaacat ttttcattac caaaatattc gagtcgcgtt tcttatgctt
    14881 cgatctctgt aacgatacct gaacctactg tccgtccgcc ttcacgaatt gagaatgtcg
    14941 ttccgttttc aacagcgatt ggatggatca agtcaacatc aatcgtcacg ttgtcaccag
    15001 gcatgaccat ttctgtatct tctggcaatg tgattgttcc tgtaacatca gttgtgcgga
    15061 agtagaactg tggacgatag ttgttgaaga atggagtatg acgtccacct tcttcttttg
    15121 tcaacacgta cacttctgct ttgaattttg tatgtggagt aatggaacct ggtttagcaa
    15181 gtacttgtcc acgttcgata tcgtcacgtt ggatcccccg taacaacacg cctacgttat
    15241 ccccagcttc cccgtaatct aacgttttac ggaacatttc tacacctgtt acaactgctt
    15301 tttgtgtttc aggtttgatc cctacgatct ctacctcatc accgacacga acagcaccac
    15361 gatcaatacg acctgaggca acagttcctc gacctgtaat tgagaagacg tcttccactg
    15421 gcaatagtaa tggtttatct gtatcacgtt ctggtgttgg gatatattca tctactgtat
    15481 ccatcaattc cataatagca gcttcagcat ctggatcgcc ttgcaatgct tttaatgcgg
    15541 aacctttgat cacaggagtg tcatcgcctg ggaaaccata ttcgctcaat aactcgcgaa
    15601 cttccatttc taccaaatcg atcaattctt catcatcgac taaatcaact ttgttcaaga
    15661 aaacaatcag gtattttaca ccaacttggc gtgacaataa aatgtgttca cgtgtttgag
    15721 gcatcggacc gtcagttgca gaaacaacta agattgcacc atccatttgt gctgcccctg
    15781 taatcatgtt tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg tagtggcgtt
    15841 tttctgtttc atattctacg tgtgcggtat tgatcgtaat cccacgttca cgttcttctg
    15901 gagctgcatc aatactagca tagtcttgag gatttgctag gccttttttg cctaatactg
    15961 tagtgatcgc agcagttaaa gtagttttcc catggtcaac gtgtccaatc gtaccaatgt
    16021 taacgtgtgg tttacttctg tcataatgtt cttttgccat aatttacaaa cctcctaaat
    16081 aatatagatc actgaaaaat ttcagctctc acctttttat tatagtcttt ttttgactaa
    16141 atcaaaaaga atacgtccga cttacttttt gtaagattcg aactaagctt attatacgtc
    16201 aatattggtc aaagtcaact aagagcactc gtctgtctta tacgcgaaaa aagtaccttt
    16261 tataaataaa aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa ctttcttttg
    16321 aagcaataag aacaaaatga ggaaaagttt aaaattcagc tgttgccaac aactttcaat
    16381 agacgaaatc ttctatttcg ttaataataa ggaaagaggt gaagtacatg aaaaaattaa
    16441 ttggcgtact tgtcgtttta ggcatatttc tcggcggaag ttttgcagct tatcattatt
    16501 tttacggcgg cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg tcagctaccc
    16561 aagcagataa cggagagaaa ttcacgactt atacctacaa gcaagcggct tataataaag
    16621 atggtgaaaa aaaacaggtg acgcttcgcg aagaaagaga acatccatta aagatgaatg
    16681 cgtatcttaa actcaaagtc aatcctcgta aaggtgtatt aagctgggaa gaaataaagg
    16741 cttctgaagt gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg gtcttaaaat
    16801 tttattagat aaaaagaggc tgggacagaa gcgtttaact ccaagaaata agaaggagtt
    16861 gcttctgctt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
    16921 gtctcacgct ctttttttgt tatattcgtc ttttccgatc cttctcttga cacaattcat
    16981 ttaactgatc actggtactt gaacacctaa tattctagtc atttttataa acgattcaat
    17041 acttcatcca catttttgat catcacggat atcgtaccat ccggattatt cacaaattca
    17101 atcaaatctg ggttgcgata tacatctact ggtacgatca gttcgatccc gtttgatagc
    17161 ttgaattttt gttttccaaa ttttttctca gaaatttctt gtacttcttt tacaggaggt
    17221 gcttctggga caaatccttg ctcttgtact tcttctttaa aagccatttt tgccgtgatg
    17281 ttttccttga agactttttc cgcaatcatc tcatgatcca accgaccttt ttcttctatc
    17341 gtatcataga cagcttcttt caaatcagcc atgatatcaa attcttctgt ctcaaatttt
    17401 tttccaagat gcttggctac tttttggatc acttttacat tttcttctaa agaaggtgca
    17461 ggaacacttt cgatcactct tccagaaaaa taaagctctt ttttgccaga aaactcatat
    17521 tttttttcaa ttaattcgta ggtccaatca gtcaaattca cagcgaatgc ttcatctgct
    17581 ttttgtgttt tcgcccctag aattgctcga ttcaaaatca agcggttgtc gatccctgct
    17641 tcgtctgctt caacaaagtg agtgtaagcc tcacgataat tgactttcaa taaagataag
    17701 tacatcactg tatctaattc atacaagaca acaaatacat ctgcactagg tgcttcttca
    17761 ctttcttggt agatatcgaa ccaccgctga acgattgctt cacttgcttc cacaaaattt
    17821 tcttgagcga ttttaagtga ttcaacgatc atgctttctt ctgctagttg tccagttttt
    17881 gtttgagcag aagagatttt ttggatcttc ttttgaagaa aatcacgaat atattctttt
    17941 gtcagatcca gctctttttg tgaaaagact ggagaaccag tttctctgtc aataatatgc
    18001 agaattgctt tttttaaata gatatccatt ttctcattcc cttctgtctt agtttacgga
    18061 aaaaaaatca aaaataccag aatcaaaatc attttctttt aaacaaaaat ttcaagagta
    18121 gaaacaaaat ggattctttt tttcgaaaaa tgcgctacac ttatagttga acgaataaaa
    18181 aaggagctga aagtatgaat tcattaacaa gcacttatcg tctatcaaat ggttacgaaa
    18241 tcccggtagt aggattcggc acatggcaaa cacctgatgg ggatgtcgct gtttcctctg
    18301 ttaaagaagc gctggctgca ggttatcgcc atatcgatac tgcccaagga tacaagaatg
    18361 aagaaagcgt cggccaagcg attaaggaaa gtggaattcc tagagaagag atattcttga
    18421 caacaaaatt atggaatgca aaccacagct atgaacttgt gatgtcttct tttgaagaat
    18481 cattgaagaa gctccaaaca gattatttag atctgttttt gatccactgg ccaaaccctg
    18541 ttgcttttcg tgataattgg gaacaagcaa atgcagatac atggcgagca tttgaagaac
    18601 tgtatgaagc aggtaaaatc aaagcaatcg gtgtgagcaa tttcttaccc caccatctag
    18661 atacactagc aaaaacagca aaaattatgc cgatggtcaa tcaagttttc cttgcaccag
    18721 gcgaattaca gacaactgta gttgagtatg caaaaaaaca tgatatgatc ctcgaagcat
    18781 atagtccatt aggcactggt aaaatcttcg atgtgcctga aatgaaacaa atcgctgaag
    18841 cacatgacaa gacgattgca caagttgctt tgcgttggtc gctgcaacac gaattccttc
    18901 ctcttcctaa gtcagtgaca ccaagcagaa tcaaagaaaa tactgaatta tttgattttg
    18961 aattaacaga agaagaaatg aaacaaatcg atcagttaga tggtgtagtc ggcaaagcaa
    19021 aagacccaga tactacccaa ttttaagtcg aaaggtgatt ttatggatcc aatcgaacgc
    19081 ttgaattctc tctcggaaga agttacccag acttttcatt ccgattttgt ctttttgatc
    19141 gatgcagaga aaatccaaca ttttcctgca agaaactgga cacatgatca aatcatcgaa
    19201 gaactgaaaa aacggttcga tcattcgcta atggtcaaac cctggcacga acatgaagtc
    19261 atttattcac ctgaactacc tgtttttgcc ttgataccga aaaaatagaa agaatggaac
    19321 ggattatg
//
LOCUS       DAFEHS010000052        19312 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFEHS010000052
VERSION     DAFEHS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.053"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            168..362
                     /locus_tag="LP327_001877"
     CDS             168..362
                     /locus_tag="LP327_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725987.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="LP327_001878"
     CDS             631..930
                     /locus_tag="LP327_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1725988.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="LP327_001879"
     CDS             complement(1027..1461)
                     /locus_tag="LP327_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1725989.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="LP327_001880"
     CDS             complement(1693..2403)
                     /locus_tag="LP327_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL1725990.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="LP327_001881"
     CDS             complement(2393..3253)
                     /locus_tag="LP327_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBL1725991.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="LP327_001882"
     CDS             complement(3258..3896)
                     /locus_tag="LP327_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBL1725992.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="LP327_001883"
     CDS             complement(3911..4210)
                     /locus_tag="LP327_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1725993.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="LP327_001884"
     CDS             complement(4253..5737)
                     /locus_tag="LP327_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL1725994.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="LP327_001885"
     CDS             complement(5757..6218)
                     /locus_tag="LP327_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL1725995.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LP327_001886"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LP327_001886"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBL1725996.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="LP327_001887"
     CDS             7521..8291
                     /locus_tag="LP327_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL1725997.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="LP327_001888"
     CDS             complement(8564..9391)
                     /locus_tag="LP327_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1725998.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="LP327_001889"
     CDS             9959..11194
                     /locus_tag="LP327_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL1725999.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="LP327_001890"
     CDS             complement(12193..12453)
                     /locus_tag="LP327_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL1726000.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="LP327_001891"
     CDS             complement(12480..13781)
                     /locus_tag="LP327_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL1726001.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="LP327_001892"
     CDS             complement(14073..14531)
                     /locus_tag="LP327_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL1726002.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="LP327_001893"
     CDS             complement(14544..15875)
                     /locus_tag="LP327_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBL1726003.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="LP327_001894"
     CDS             complement(15886..17328)
                     /locus_tag="LP327_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL1726004.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="LP327_001895"
     CDS             complement(17315..18079)
                     /locus_tag="LP327_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBL1726005.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="LP327_001896"
     CDS             complement(18183..19058)
                     /locus_tag="LP327_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL1726006.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6154 a         3959 c         2960 g         6239 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat ta
//
LOCUS       DAFEHS010000053        18895 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFEHS010000053
VERSION     DAFEHS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.054"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(21..251)
                     /locus_tag="LP327_001897"
     CDS             complement(21..251)
                     /locus_tag="LP327_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726007.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="LP327_001898"
     CDS             complement(687..1253)
                     /locus_tag="LP327_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL1726008.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="LP327_001899"
     CDS             complement(1253..2209)
                     /locus_tag="LP327_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBL1726009.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="LP327_001900"
     CDS             complement(2209..2865)
                     /locus_tag="LP327_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL1726010.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="LP327_001901"
     CDS             3235..5253
                     /locus_tag="LP327_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL1726011.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="LP327_001902"
     CDS             5680..8094
                     /locus_tag="LP327_001902"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBL1726012.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="LP327_001903"
     CDS             8442..9995
                     /locus_tag="LP327_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL1726013.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="LP327_001904"
     CDS             complement(10376..12160)
                     /locus_tag="LP327_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726014.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="LP327_001905"
     CDS             complement(12175..13896)
                     /locus_tag="LP327_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726015.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="LP327_001906"
     CDS             13996..14757
                     /locus_tag="LP327_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL1726016.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="LP327_001907"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="LP327_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18895
                     /locus_tag="LP327_001908"
     CDS             16664..>18895
                     /locus_tag="LP327_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726017.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3353 c         3786 g         5551 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DAFEHS010000054        18273 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFEHS010000054
VERSION     DAFEHS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.055"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            66..959
                     /locus_tag="LP327_001909"
     CDS             66..959
                     /locus_tag="LP327_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBL1726018.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            1093..1218
                     /locus_tag="LP327_001910"
     CDS             1093..1218
                     /locus_tag="LP327_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1726019.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1352..1984)
                     /locus_tag="LP327_001911"
     CDS             complement(1352..1984)
                     /locus_tag="LP327_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726020.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(2132..2674)
                     /locus_tag="LP327_001912"
     CDS             complement(2132..2674)
                     /locus_tag="LP327_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBL1726021.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            2809..3198
                     /locus_tag="LP327_001913"
     CDS             2809..3198
                     /locus_tag="LP327_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726022.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3202..3966
                     /locus_tag="LP327_001914"
     CDS             3202..3966
                     /locus_tag="LP327_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL1726023.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            4095..5042
                     /locus_tag="LP327_001915"
     CDS             4095..5042
                     /locus_tag="LP327_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1726024.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            5172..5912
                     /locus_tag="LP327_001916"
     CDS             5172..5912
                     /locus_tag="LP327_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726025.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(6203..6871)
                     /locus_tag="LP327_001917"
     CDS             complement(6203..6871)
                     /locus_tag="LP327_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL1726026.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            7005..7292
                     /locus_tag="LP327_001918"
     CDS             7005..7292
                     /locus_tag="LP327_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726027.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            7708..9246
                     /locus_tag="LP327_001919"
     CDS             7708..9246
                     /locus_tag="LP327_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBL1726028.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            9243..10208
                     /locus_tag="LP327_001920"
     CDS             9243..10208
                     /locus_tag="LP327_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726029.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            10195..11517
                     /locus_tag="LP327_001921"
     CDS             10195..11517
                     /locus_tag="LP327_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL1726030.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      11580..11684
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11758..12501
                     /locus_tag="LP327_001922"
     CDS             11758..12501
                     /locus_tag="LP327_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1726031.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            12516..13508
                     /locus_tag="LP327_001923"
     CDS             12516..13508
                     /locus_tag="LP327_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL1726032.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            13520..14257
                     /locus_tag="LP327_001924"
     CDS             13520..14257
                     /locus_tag="LP327_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726033.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            14247..14969
                     /locus_tag="LP327_001925"
     CDS             14247..14969
                     /locus_tag="LP327_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL1726034.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(15053..15676)
                     /locus_tag="LP327_001926"
     CDS             complement(15053..15676)
                     /locus_tag="LP327_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL1726035.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            16071..16762
                     /locus_tag="LP327_001927"
                     /pseudo
     CDS             16071..16762
                     /locus_tag="LP327_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            16970..17260
                     /locus_tag="LP327_001928"
     CDS             16970..17260
                     /locus_tag="LP327_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726036.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         6265 a         2996 c         3685 g         5327 t
ORIGIN      
        1 gttcttcctc ctttttacgt aaaagccttt taagtaaaat aaaacggata gaaaagagaa
       61 catatatgaa ctattattca aaaaataagg aaaactttta tcaaaaactg accgatgatc
      121 cgcttttttc ttcattgacc gattatctat atgaacatcg agaacaagaa acgattctaa
      181 gagaattgaa aaaagaattt tcgcaaaata aattttccca ttttcttgat ttattgatcg
      241 atgcaggatt aatcaagagg gaagaacgaa gatatcattt gaactttcca atatttgatc
      301 ctaaagatta cttgcaacaa gccacttcgg ctgctgaaac catagctgaa caattgaaga
      361 gactctctgt tgatgaacaa aaattagcga tgggagaagt tatttgggcg tattgtttcg
      421 aagatgaacg gaaagaagcg tatttttatg gagtaagaaa cagccgagag acagaattgc
      481 tgaggacaac tgcaggaaat gagaagtacc gttttatcac actttcctct atagaacact
      541 tcccgcttac tttggctaat tacttttttg tacagaagaa tcagcttcct gtaacgaagg
      601 cattcaaaga attagccgaa cttattggag acgtaaatga atcgtacttt tttgatcaaa
      661 tagaagttat cgttgacagg atacgtaaga ataaatataa aaatcgccgg cctagtattt
      721 tccatcagtc tctacttgtg acgaatacta taaaggaaga agaatcattc acattagaat
      781 tacctattgt cgaaaaaaac aattttgaga ttgaattacc aacattggat ccttcattga
      841 caatggagga aatggccttt cttaaacgac aaatcttttc tgagctgagc aagaaattta
      901 taccgcatgc attttcttat atcaaagaat atggaacagt tttagtgagt aaaacctaag
      961 agagcagtaa gcaaaaatta tactgactct tgcaggagga gcctttctct tcctttatgg
     1021 ttaaaagaat tatttgagta ttttgcctat ctaccaccta ttagattcac tacatggtct
     1081 ggaaacaaag gaatgcaaaa aaatggtgag aaatgtggaa tgacaaagga agtagtgata
     1141 agaaaagtta gatttttaaa taatcaatat tatgattcag tcaagtatgg cattttatgg
     1201 gaagaactag ctgattagat taggaagtcc atttgataat ccagaatcac taagaaacga
     1261 atggcgagtc attaataatt tatccaatct tgggttattc ttttagattg aagtgtttaa
     1321 aactaaaaaa gtatttatga agtcgtaata gtcaggcttc aaaatgcttt ttattttgtt
     1381 tgttttttct ttgccacata aatacaccta gactgacgat gaagagcata tccaacagat
     1441 ttcctgcgac ttggatcgtc gaagggttgg tcagtggcat taaagcaagg acaaaaaagg
     1501 aaaacaagct gtaaataaaa caggtgacag gaactaatcg aaagaagagc ggacatttca
     1561 attggctaaa aatgagtgaa aaaataaaat aagtaaaaat ggataaaatc acgactaaac
     1621 cattcatgat aaagaagaaa agcgtattgt gattaaacga ataaaaaaga attgctaaaa
     1681 aagaaaacgt tttaaatgca atcaatacgc ttgaagcttc gtttaacgta ccaggatgtc
     1741 gatgaaaaaa ataggcaccg aaataaacag aaaacagtaa gccgcaaaga gaagacaaaa
     1801 tcaaaaaaaa cggattttct tcggcttctg tgatatcaga atgatccaaa agcaaccagc
     1861 ctaatgcact agaaggcgca tgtgaggtct taaggtatga ataacataga aaaaataaga
     1921 tcgtcaaagt tgcaaaaaaa gagcctaaat gtaaaaaatt agtggttcta cgttgaattt
     1981 ccataatatc tccaaattaa attgataagt atataaagta taataacaaa ttgatgagta
     2041 tatgtcgatg ttttttaatc aaaataataa ataaaaactg tctaacattt aaaatttaat
     2101 ttagatatta gacagttttt aagtttgtga gttattttcc ttgtcgtttt ctcacttttt
     2161 tagtccagaa accacctgaa atatattttg gctcataaga aatgataaaa gcacgctctt
     2221 cgatttcttt gatcaggcga tacagttcac gttcgctttt tctaggagaa agtatttcga
     2281 gaactagccg ctcaccttcc agccccattg cagaagtctg tgtcacacca taaccgtgtt
     2341 ctctcagtat acccggcaaa cttttatctt ctgcgctatt tgctggcaaa atcgttgtga
     2401 ccatgatgta tcctaatgcc aatttgtctt caatcaaaat tccgacactg atacctactg
     2461 cgtaacctaa cgcatagaca aataaattca gtggattatc taaacgatct aaaacaaggg
     2521 ataagccgac gatataaatc gtgatctcga ccatactgac aagtggagca atcagtcgat
     2581 agcctttcat tgtcaacatg aaacgtaacg tgttcagagt gatataagca aaattaatga
     2641 ggaagatcat ccccaacatt tttggatcaa tcatttgtaa aactcctttc atttatataa
     2701 caacaatatc aaaaaaaatc gtaagtgaag aaaaattgaa actttataca tacaaaaaaa
     2761 tgataaaatg attataatag aattataaag ataggttagg tgattaaaat gaaaacggtc
     2821 aatcgtgtgt tttgtgccag ccacgtgcta agtctccttc tcagtatata cttaggtgtc
     2881 tattcgattc aacagccaat tgaggtagag aacattgttt ttcagatgtc gctgacagat
     2941 ggtttaattt taagcgtttt tttctttttg atactgttta ttggtaatat tttcggtagc
     3001 gttgccagtt ttttgaattt ttctatttat ccactccttt cgctagcatt aggagtggtg
     3061 ggactttgtt ctctgtgctt atttcccgag ccattcagtc ctgcatttat attatttggt
     3121 acactgaacc tgttccagac aactgttgga gcatggttgc tatggcgatc aggaaattta
     3181 atgaaaatag gtgagtaaca attgaatcca attatattga aacgttctgt ccatcatatt
     3241 ccagtgttag aagttgttct gaaagaatta agagaggaac gtattcccgt tgtcatctat
     3301 tatcacggat ggcagacaag taaggagtta gttttgacgc aaggaagaaa aattgcgcag
     3361 cagggcattc gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa acagccggtt
     3421 tcttctattc catcctatac attttgggat agtatctacg gaaatctgtt tgaatttgac
     3481 acacttatca agcatttaga aaaaagaggt cttctacatg atcagcttgc agttggcggg
     3541 gtttcgatgg ggggaatgac aacatgcgcc ttgttggcta aacatcctca tatagtagcg
     3601 ggcgtgtgtt tgatgggttc ccctgcacct ttggaatatg gaaaagaaat cgtaaggcgc
     3661 gcatcggaat ataattatgt cgtaccaaaa gactattttg atttgatttc atgggttaag
     3721 agctatgatt tgtcattgca gccggaaaag ttagctggac gtcctctgtt cttttggcat
     3781 ggacaagctg acgaaaaaat cccttatata caaacggaac gatttgtaga aaaaaatcgg
     3841 cataagtctt acggaaaaga aattgttttt cgttcctcaa aaacacagcg acatatggta
     3901 caaataccac taatggaaga agcttccact tttttagcaa aaaatttgat aaaaaagtat
     3961 aaataattga aacggaaaat cactcatgac gatagaggat tttccgtttt ttattttggg
     4021 ctataataaa ctctgttagg aaaatttggt ataattgata gtaagaatga caacatgtta
     4081 gaaaggaaga gagcatgccc actttagcag atgttgcaaa acgtgccaat gtttcaaaaa
     4141 tgacggtctc tcgagtcatc aatcatccgg aacaggtgac tgacgaattg aaagagttag
     4201 ttttctcagc aatggctgaa ctggattacc gtccaaatat tgctgcaaaa gcacttgtca
     4261 cgaatcggtc gcagatcatc aaattattta tactggaaga aatcgacaca actgagccct
     4321 attacatgaa tttgctgatg ggaatcgcta aacggatcgg taaagcgcat tattctctcc
     4381 aattagttac aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc atcacaggtg
     4441 tcagaaaaaa agacttcgat tggatcgacc gtttggaaaa accagttgtc ttgtttgggg
     4501 aaaattctga aggatatgat tatgtagaca gcgataatga atatggtaca gcaaaagcaa
     4561 cgcaatatgc cttagaaaga gattatcaaa cgattattta tatcggtatc gaagtggacg
     4621 agccttttga aatttcaagg gaaactggtt atttatctgt gatgaaggaa gttgatcttc
     4681 ctagtaaaat cttgcggttt ccaaatcatt cgacagaagt agaaagatat atgacagaaa
     4741 actgggcaga ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg ctagcactag
     4801 gagcagtaag agggattata aatgctggag gacacttgcc cgaagaattt ggtgtgattg
     4861 gatttgatgg ggtattttta gaccaaatca gttcacctaa attgacaacg atcaagcaag
     4921 atattatacg tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata gaagaaaaag
     4981 gggcgagtca aggttatcgt cgtttttttc ctgaactagt tatcagagag acaacgaatt
     5041 aagtcttatt actatatata tatttatgaa attcatcgct attttcttaa aataatcagc
     5101 gcattgctac tgaaaacaat gaatctgtgc tattattgag aaaagttcca tttttgagaa
     5161 ggatggtttg aatgaaagaa atcagacgaa aacatttccg ttttcctgcg tacgatgatg
     5221 agttaggcgt aaaattaacc aacgacaaga agcgagtatt atttgacgga caagaagatc
     5281 ttttgactga acagaatcag tccccgtttg aacagatgaa agaatttcat ttcacgaaca
     5341 cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag tcagctccgc
     5401 gtaaggaaaa tttggaaaag cataaaggaa aattaccgga ttataccggc cattaccaat
     5461 cagaaatcac atcgacagga cgaaaaaaac tttttggtga ttcctctcgt tctgtcagac
     5521 aaagtgaaac aaggacagag tccaagcata ctacacgaaa cgcatctgtt gacacgaagg
     5581 tgcgggagcg ttcttatttt gttcctaaat atgtacccgc atcgattatc ccagacccta
     5641 gagaaagcaa aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg gatgactctt
     5701 atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt gagccgtctg
     5761 tacgaacatt caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa gaaaaaaaat
     5821 cgaaaaaaag ccatggtatc ttagaacgct cattacaagg attgatcgat gatcaaggaa
     5881 actctttggg agaaaatagc tattttaaat aagtgatata caaatttata ttcggctaca
     5941 gttggaaagg aaacgctgcg gcgtttcctt attttatact ctatccgaaa gtttatatcg
     6001 tatcctcaaa tttttttgag tttaagcgaa ttttttctcc caataagtaa gaaaataatg
     6061 gcaaaataaa aaaacaaact attttcgcag atcgtttgca aaaatagttt atttttttat
     6121 aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc tgtaaattaa
     6181 ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca agtggttcaa
     6241 atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt tccagatact
     6301 tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac agaaaatgtg
     6361 tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag aaaagacttt
     6421 tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct gttccgcctg
     6481 tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata cctgtcatca
     6541 aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc cataaatgta
     6601 aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt tttccaaaaa
     6661 gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca gaaggagcga
     6721 atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct gccagcttag
     6781 atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa atgattccgc
     6841 acaattctgt aaataaattt aattttttca tttctagcct cttttctttt gaaaattttc
     6901 agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg aaaaaaaatt
     6961 atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga gcagcagact
     7021 tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt tttttggctc
     7081 aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat caatttatct
     7141 ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt tttttgatga
     7201 agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct ctttatggag
     7261 tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa gacgtttatg
     7321 atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac agtataatat
     7381 tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta ttggctgttt
     7441 ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc aagtgacaat
     7501 ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag ttgatccgac
     7561 tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc ttttttttgt
     7621 tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa atgtttatac
     7681 ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt aagtgaaaga
     7741 aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat ttctttgctg
     7801 attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa aaaaatggca
     7861 gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc cttattaggt
     7921 ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact tgttatttac
     7981 gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga cccgtctatt
     8041 gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa agtagagctt
     8101 cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt aatcatcggt
     8161 acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat tttgttaggt
     8221 attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc cttgcttgca
     8281 attatattca gcggactgat tcgctggttg cagcataaga aacctcgata ttccatcatt
     8341 actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc tgccttgttt
     8401 aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat cctgatccaa
     8461 atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa accgaatttt
     8521 ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat ctatcctgag
     8581 ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa aagtgctgat
     8641 ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca attcgatatg
     8701 acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa gagatctttt
     8761 gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga tcagatcaaa
     8821 gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat ccaagaagca
     8881 tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta ccaagcaatc
     8941 aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct caaggagtac
     9001 gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg cgcaccgctg
     9061 atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa taaattaagc
     9121 ggaaaaataa cagaggatca aatgattgat atgaattacc aagtcaatgt cgaaaagaaa
     9181 aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa ggaggaagat
     9241 cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga aagtatgtca
     9301 tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt ggtccttccg
     9361 gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca tcagcaggag
     9421 acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg cggtggaata
     9481 tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag caaaatattg
     9541 aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt gtagatgaac
     9601 ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct agagagttgt
     9661 ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc ccagatgtga
     9721 ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca ttacaagaat
     9781 tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg cacaatatgg
     9841 aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa attatccaat
     9901 gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg catttctttg
     9961 atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat ggatatttct
    10021 taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct atgaaagaag
    10081 tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa agaagtattg
    10141 ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa ggaaatgaaa
    10201 acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc ttatggattg
    10261 aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg gacttgtcct
    10321 aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg taatcgggta
    10381 aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga agaacttcaa
    10441 aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca attagcagaa
    10501 gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc tattgaagtc
    10561 aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag acctacgatc
    10621 ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc attgcctgta
    10681 ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct agctacgatt
    10741 gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc attaaaagaa
    10801 tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg cattcagttt
    10861 gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg tttagtcgga
    10921 aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca gccgaataca
    10981 gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat tgcagtcaat
    11041 gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt ttcaaggaaa
    11101 accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa gcggataacg
    11161 gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta cgccagtcct
    11221 aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca agtggtagag
    11281 atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc taaggctgaa
    11341 ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga gcaagcagac
    11401 gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa agagttggat
    11461 agctatatca tggggtatcc gacattggca agtgatctct cctatttatt gaaataatca
    11521 gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta aaaaatgaat
    11581 acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta gacccattga
    11641 acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc gggttactat
    11701 gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg taacaaaatg
    11761 aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg gcagattgtt
    11821 gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt attttctgca
    11881 ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa tgaagcagtg
    11941 cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat ggattattgg
    12001 aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt gccaatcatg
    12061 gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa agtgatcctt
    12121 gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt gaggaaagta
    12181 tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa gatttatcga
    12241 atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt ggctgctact
    12301 tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg cttagggtat
    12361 tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc gatcatttgt
    12421 gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa agggtattat
    12481 cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg cttttattac
    12541 cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc aaacaagaaa
    12601 taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact aatcatactg
    12661 gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat gtccaaatca
    12721 tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa gcacaatttg
    12781 gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat ataccagtca
    12841 aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta tcagacagta
    12901 acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa agtccaagtg
    12961 aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca aaactgacaa
    13021 tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat atccaatggt
    13081 actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta aactatatcc
    13141 cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt acgaatgatc
    13201 aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg aaaaaaggct
    13261 atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa tatgcaaaag
    13321 aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat tatacggatg
    13381 atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca gcttttatgc
    13441 aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact aatcagttta
    13501 taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag tcattgaaaa
    13561 cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta catcgattgt
    13621 cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag tccagcctat
    13681 gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata cagcatttag
    13741 ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa acgttacgtt
    13801 atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt atctggatat
    13861 ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg ggatgaagca
    13921 gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc ttgatgaacc
    13981 atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt tgaagctgcc
    14041 taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag caatttattt
    14101 atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg agattcaagt
    14161 gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga agcaaacgat
    14221 ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat ttagatggga
    14281 gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc aattgtgatt
    14341 cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa gccttaagtt
    14401 acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag ccttttttga
    14461 aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat gtaccgatga
    14521 ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat gagaaacctg
    14581 ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa gcttacccta
    14641 atcactattt gatccattgg tattcttctt tggactatca acaagactat attgatttag
    14701 gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg caactagcta
    14761 aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca gcagagtggg
    14821 ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa cagttaaaga
    14881 caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact tatcaaaact
    14941 tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta gtgtcgcacc
    15001 tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa tcttagtacc
    15061 aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt gaagtaacat
    15121 attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag taagaagaat
    15181 ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg cttgattctt
    15241 tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca gtttctgtta
    15301 cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt tgaaccgttt
    15361 cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct tgaggttcag
    15421 cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag taaatcaggt
    15481 taatatcaga aattgaattt gattctgcaa tggcattgat caaggagtta tcaccaacgt
    15541 atttttgaga aatcgttgat agagtatcac ctgattgaac ggtataagct tcgtccgcat
    15601 gagcagtcgt tcccatgaag aatgcagcgc cggcagccaa agttgttcca aaaagtaaag
    15661 ttttaagtga tgtcatgaaa aatctccttt attcgttaaa aagttttatt tgttgtgttc
    15721 aacgaggaat actttaacac gtgaaaatat ttcataggta aaagaagcat gacaatttat
    15781 gacaaatccg cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt
    15841 aataaaactt gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc
    15901 tgcatttttc tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta
    15961 tttgatagta tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg
    16021 agatcactta cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga
    16081 aatttattac tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc
    16141 ggtacgcaaa tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga
    16201 atctgacaca gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg
    16261 ctcgactagc acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc
    16321 aaacaaaagt aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact
    16381 tagaaagtca attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg
    16441 tcaaactaga tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga
    16501 cagcaagact acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt
    16561 tgaatcaaca aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaatc agttaacaga
    16621 tacacaaaag aaattagacg aagcaactgc tgaaaactca agcttgaaac aatatattca
    16681 aaagttagaa aaagcaaaag atgaagtgga agatacagct gataaatctc agcaaattgt
    16741 tgaagaacat ttgcgtaaat aatctttctt aacaagtaaa cctaaattag taaagaagca
    16801 acagtaatct atactgctgc ctcttttttt tagtacatgt ccttcatctt cgcaggaagt
    16861 gccaatatga ttttaagaaa ggtaatagac aaaaagtcaa aaagataaaa cagagtttct
    16921 tctttacaaa gggaatgtat gttcgtataa tggtattgag gtgaaggtta tggagaaaaa
    16981 atatggaaca gtagataaga tccgtacttt gcaagttagt gccagaccgt tgatccgttt
    17041 cagtttaaat ggggtcaatt gtttgattgc ttcccatagt ttgaacttcc tagctgaagt
    17101 agacgaaggg atgaagttag tcgttacagg ttattataat aatagaaagc aatttgttgt
    17161 gagatcctat catgtgttag ggaaacccaa aattgtggta gaatatgaga aaagtctata
    17221 cccaagaaaa aaggtccgac aagatgaaag aataaactag aaaacaagcg gctgagacaa
    17281 aaatgtccta gccgcttgtt ttggaaagat ggatgcagac gatgggattt gaacccacac
    17341 gagcatacgc tcacaagaac ctcaatctat atataactac atgtatttaa gtatttgtga
    17401 tgaatcacta aactttccaa actcatttga gggatattgc ttaaagattg aacagatcaa
    17461 ttcgaagccc tattagtatt cgctcaatgg ggggatcaga ggccataaaa agagactagt
    17521 cattactagt ctcaggtagt ggaattgtag gattttaaac gttcctggag agtaccggta
    17581 tgtaactcga agaggtgatt atcatagtcg taaaagtaaa ttgaagaagc ctcgcctttc
    17641 acacgagaac gtcctggttc gacagttaaa cctaacattt ggatttttga gacaaacgaa
    17701 tctatatctt gttcgtcaat tttaaaagca atatgattgt atgtctttgg cagtttattc
    17761 gacgaattct gcattactgc aatccatagg tccttaacta agaaaaattt ttctggatat
    17821 aatgaaaatt tttttgttga ctatcgtaga tttcttttgc atcaaataag gtatttaaca
    17881 attaagtatt taaaagataa cttttaagtt atctttaagt aatgcttaaa gctatgttat
    17941 actctattag gaaatataag aaggagtata aaaatgaaca aacataatag aaaaataacg
    18001 aataacaaag aactgatctt gttactaata gggatttggg gtatcttttt actctttaat
    18061 atgtatatgc cgactatgag agcggatgac gtagtttatg caagtagatt agatgaactt
    18121 ggctatttgg gtgcttcgat agaacattat aagacatgga gttcaagaat tattatagaa
    18181 ctattcttaa tgttcttttc taaacacttt atgctatgga aattattaaa ttctacgatt
    18241 atgttaggaa ttgtagtatt actttgcaag tat
//
LOCUS       DAFEHS010000055        17742 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFEHS010000055
VERSION     DAFEHS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.057"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            4..816
                     /locus_tag="LP327_001929"
     CDS             4..816
                     /locus_tag="LP327_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBL1726037.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="LP327_001930"
     CDS             813..1613
                     /locus_tag="LP327_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBL1726038.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="LP327_001931"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="LP327_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBL1726039.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="LP327_001932"
     CDS             3110..4819
                     /locus_tag="LP327_001932"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBL1726040.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="LP327_001933"
     CDS             5031..9389
                     /locus_tag="LP327_001933"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBL1726041.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="LP327_001934"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="LP327_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBL1726042.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="LP327_001935"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="LP327_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBL1726043.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="LP327_001936"
     CDS             11227..11520
                     /locus_tag="LP327_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBL1726044.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="LP327_001937"
     CDS             11517..11828
                     /locus_tag="LP327_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBL1726045.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="LP327_001938"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="LP327_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBL1726046.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="LP327_001939"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="LP327_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBL1726047.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14678..14851)
                     /locus_tag="LP327_001940"
     CDS             complement(14678..14851)
                     /locus_tag="LP327_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1726048.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15299..17661
                     /locus_tag="LP327_001941"
                     /pseudo
     CDS             15299..17661
                     /locus_tag="LP327_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
BASE COUNT         6057 a         3155 c         3899 g         4631 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag cagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtggtc acagcagccg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcagtaata ggagtttacg agctgtttcg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtattg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattta atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg ttcgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcctgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccggataaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcgt attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct acaccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gattatcgga gacggtggcg caatgggcgg
     3721 gaaagactca aaagaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgcggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtactttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaat ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tcttcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtgga acaacattca gatgaacaag gaatcaactg
     4501 gccaaaagga attgcgccat ttgatttgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaaac ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gtgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctgcatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggggttgcg ggattttttt gttttcatta
    14581 agacgtatta cctaagagtt ccatgacttt agtcttgcgg cgtgtcagct aaccaaaaaa
    14641 gagaaaaaga aaagtaaaaa attcgacttt cttttttcta gtagtatata aatttttgca
    14701 tatagataaa gatcaagata ccgataacgg aagctagatt gtaatctatt ttagtgacag
    14761 tcaggtatag ccaagtaact agaagatcag tggaacctgc accataaaag tttgagtttg
    14821 tcggtccgtt gacagttaaa agaaaaatca cgttgaagtt attgatattt ccgattacgc
    14881 cgcttcgatt tggtcacgat ccaaattttg gataatccct gtagaaatta gcatagtagc
    14941 tgggatacca attcacatat tgacaaagat aacagagact tttacataaa acggatcaat
    15001 caggaaagga attgctcgat ccagccccag ttttccagca atgtgttaac tggcccagcg
    15061 tcattcagca aattatgcat gatgaggagc gaaatgaact gcggaacagc cattgtgata
    15121 acgacaagtt ttttggatca atgtaaaaaa cgtcagcgaa aggaaaaatt tcgtggtttt
    15181 ttatacctgt tcatttggct agatataaaa tccaaacaaa tgaacagaag tgcttattag
    15241 gtttcaattg ggagtatctg cgctagaata agagtaataa tgaaaaggag tgttatgtat
    15301 ggattattct agtaaagaat actttgataa aatgacggct tggtggcgtg ctgctaatta
    15361 tctttctgtt gggcaacttt atcttaaaga taacccctta ctgagacgta cactgaaacc
    15421 cgaagatgtc aaaaaacatc caatcggtca ttggggaacg attcctggcc aaaattttat
    15481 ttatgtccat ttgaaccgag tcatcaataa atatgatttg aacatgtttt atattgaagg
    15541 gccaggacac ggcggacaag tcatggtatc gaatgcttat ttagatggaa gttacacaga
    15601 gatctatcca gaagtaacgg aagatgagac aggaatgcaa aaattattca aacgattttc
    15661 atttcctgga ggcattgctt ctcatgcagc accagaaaca ccaggttcta tccatgaagg
    15721 cggagagctt ggatactctc tttcacatgc agtcggagct gtattagaca atcctgaagt
    15781 aatctctgct gtcgtgattg gtgatggtga agcagaaacc ggtcctttag ctggaagctg
    15841 gttctcaaat gtctttatca atccagtgat agatggagct gttttaccga tccttcattt
    15901 aaatggtgca aaaatagcca acccaacgat cttggcacgt aaatcagatg gagaattagc
    15961 aaattacttt aacggacttg gctgggaacc tttctttatt gaaggaaatg attcagaaaa
    16021 attaaatcct gttatggctg aaaaaatgga tcaagcaatc gagaaaatta aatccatcca
    16081 aaaagaagct cgcctgaaga cagcaacaga tgtcgtgatg ccaaaatggc cagtattgat
    16141 cgtacgcaca ccaaaaggat ggaccggtcc aaaagaatgg gacggggaac caattgaagg
    16201 aaccttccga gcacaccaag taccgattcc tgtagatcaa gaacatatgg atcatgcaga
    16261 tgctttactc cgttggctga aatcctatga accagaaaaa ttatttgatg cacaaggacg
    16321 tattttagaa gaaatcagag aaatcgcgcc tactggtgac caaagaatgg caaaaaatcc
    16381 tatcaccaat ggtggaatcg atccaagacc gcttattatg ccagattgga aaaaatatac
    16441 tttgcaattt gaaaaaccag gctccgttaa agcagaagat atgaccgaac ttggtaaatt
    16501 cgttcgcgag atcattgaga aaaatccaga aaatttccgt atatttggac cagatgaaac
    16561 aaaatcaaac cgattgaatc aagttttcaa aacgacaaat cgccaatgga tggaaaaaat
    16621 cgaaccagaa aatgatgaat ggctttcgcc atctggtcgc gtcatcgaca gtcaattatc
    16681 ggaacaccaa gatgaagggt tcttagaagg gtacgttctg actggacggc atggtttttt
    16741 gcaagttacg aatcattctt gcgagtggtt gactctatgt tgactcagca cttcaaatgg
    16801 atgcggaaat cacatgacct ttcttggcga aatgattatc cttcgctaaa tctcattgca
    16861 agctcaacag tcttccagca agatcacaat ggatatagcc accaagatcc aggaatattg
    16921 acacatttgg cagaaaagaa ggcggagttc atccgtgaat atctgcctgc ggatgcaaat
    16981 acattgctcg cagtcatgga taaagcattc cgctcgtcag aaaaaatcaa tttgatcatc
    17041 tcttccaaac atccgcgtgc tcaattttac tcagcagaag aagcagccgt cttagtgaat
    17101 gaaggcttga aaatcattga ttgggcttcg acagcaaaag aggaggaacc tgaattggtc
    17161 attgctgctg caggaacgga atctaactta gaagcattag cagctgtaac tttgctgctg
    17221 gaagaattcc caaaactaaa aattcggttc atcaatgtcg tagatttatt gaaattacgt
    17281 catccaagtc aagatccgcg tgggttgagc gatgaagaat ttgatcaata ttttacaaaa
    17341 gacaaaccga tcttatttgc tttccacggt tatgaaacat tggtccgcac gattttcttt
    17401 gatcgtcata accatcattt aatgattcat ggctacaaag aaaatggaga tatcacgaca
    17461 ccatttgaca tgcgtgtagt aaatgaactt gatcgttatc atttggcaaa agacgcagct
    17521 ttaaaaatca aaggaagtca agcagaagat tttgcgaaaa aaatggatca gaaattacaa
    17581 gaacatcaaa actatattcg tgaaaatggt attgatctgc cagaagtatt ggattggaaa
    17641 tggaaaaatc ttgaccaata aaatgataaa atcgatcccg caaaatagaa ttttttacat
    17701 tgattaagaa actgaaaaaa taaataaaaa aaacggaaaa aa
//
LOCUS       DAFEHS010000056        17272 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFEHS010000056
VERSION     DAFEHS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.058"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(33..602)
                     /gene="thiT"
                     /locus_tag="LP327_001942"
     CDS             complement(33..602)
                     /gene="thiT"
                     /locus_tag="LP327_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBL1726049.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(668..770)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(776..1747)
                     /locus_tag="LP327_001943"
     CDS             complement(776..1747)
                     /locus_tag="LP327_001943"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL1726050.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(1841..3214)
                     /gene="glmU"
                     /locus_tag="LP327_001944"
     CDS             complement(1841..3214)
                     /gene="glmU"
                     /locus_tag="LP327_001944"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBL1726051.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(3717..4535)
                     /gene="purR"
                     /locus_tag="LP327_001945"
     CDS             complement(3717..4535)
                     /gene="purR"
                     /locus_tag="LP327_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBL1726052.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(4702..5526)
                     /locus_tag="LP327_001946"
     CDS             complement(4702..5526)
                     /locus_tag="LP327_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL1726053.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5499..6179)
                     /locus_tag="LP327_001947"
     CDS             complement(5499..6179)
                     /locus_tag="LP327_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL1726054.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6202..7140)
                     /locus_tag="LP327_001948"
     CDS             complement(6202..7140)
                     /locus_tag="LP327_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBL1726055.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7385..8248)
                     /locus_tag="LP327_001949"
     CDS             complement(7385..8248)
                     /locus_tag="LP327_001949"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBL1726056.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(8390..10963)
                     /gene="mprF"
                     /locus_tag="LP327_001950"
     CDS             complement(8390..10963)
                     /gene="mprF"
                     /locus_tag="LP327_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL1726057.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11104..11910
                     /gene="proC"
                     /locus_tag="LP327_001951"
     CDS             11104..11910
                     /gene="proC"
                     /locus_tag="LP327_001951"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBL1726058.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(11989..13545)
                     /gene="rny"
                     /locus_tag="LP327_001952"
     CDS             complement(11989..13545)
                     /gene="rny"
                     /locus_tag="LP327_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBL1726059.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(13956..15005)
                     /gene="recA"
                     /locus_tag="LP327_001953"
     CDS             complement(13956..15005)
                     /gene="recA"
                     /locus_tag="LP327_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBL1726060.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15114..16355)
                     /locus_tag="LP327_001954"
     CDS             complement(15114..16355)
                     /locus_tag="LP327_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBL1726061.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(16595..17173)
                     /gene="pgsA"
                     /locus_tag="LP327_001955"
     CDS             complement(16595..17173)
                     /gene="pgsA"
                     /locus_tag="LP327_001955"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBL1726062.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
BASE COUNT         4940 a         3842 c         2993 g         5497 t
ORIGIN      
        1 attctgtctc aagtttcttg cttctttttt atttacagct cctgtcgttt atctggcaca
       61 aaaatcgaag gagtcgtttg ttggatccct aataaaacga tgatcgttac aatagctgta
      121 ctcaacccgc tggcaccatt cattacgaaa gaaaagagcc aaggattcat cccccatagt
      181 gcatacgctc cccagaaaac cactcctgca ataaaatgcc aaaaatatct ggaaagtgcg
      241 ccaatcaatg caccaaacac gatgcttttt ctaatttgtt tcgtattatc gtctacatag
      301 cctttttgga catttgaagc catgagccct gctgcgcctg caaaagtata ggcaatcggg
      361 tactcaatca acacttgaac gacacttaaa taataaactt gtcccaacgg aaaatgtagg
      421 atgccccaaa tgaaacctgc cagcatccct gcctttgtcc ctcttcttaa cgcaaaaaga
      481 gtcaatggaa tctgccctaa tgaaatagaa aaactgctgc cgatctggat cggaatcaac
      541 gacaacacca tcgctaacgc tgcaacaatc gttccttcta cccaaactct attttttccc
      601 ataaaaaaac ttcctccttt gaataaaaaa gtcacaaagg aggaagcagc tttcgaaaat
      661 agtcaaacag ctgtttattc acaattccta cgctcgcata accgatacag gtacgaaggg
      721 tttagaaata gattcaatct cagcccaatt ggactcccct ttgtgagttt atttattatt
      781 tattttttgt ttcaaacaat ggactaactg gtttgttttc atggatacgt ttgattgcat
      841 ctcccattag tccgccaaca cttacttcat cgattttgtc gattttacga tcttctggaa
      901 gatagatcga gtctgtaaca actagacgct cgatagctga atcttctatt ctttgtaaag
      961 cagggccgga taatactgga tgtgtacatg aagcatacac actagttgca ccagcttctt
     1021 tcaaagcatt ggcagccaat gtgatcgtac ctgctgtatc gatcatatca tcgatcagta
     1081 cacaagtttt gcctttgact tgtccaatga tattcattac ttctgcaaca tttgcttttg
     1141 gacggcgttt gtctatgatc gcaatcggag atttcaaaaa ttccgctaat ttacgtgccc
     1201 gtgtgacacc accatggtct ggtgaaacaa cgacaacatc atcgcctttg attccttttt
     1261 ctaagaaata attggcaata agtggcgcac ccatcaaatg atcgactgga atatcaaaga
     1321 aaccttggat ttgaactgca tgaaggtcca aagtcagcat tcttgttgca ccagcttttt
     1381 gaatcatgtt tgctactaat ttcgctgtga ttggttcccg tgcacgtgct ttacggtctt
     1441 gacgcgcata gccataataa ggcatgacta cgttgattgt ttttgcactt gctcgtttca
     1501 gtgcgtcaat catgatcagc aactccatca agttatcatt gactgggctg ctcgttgatt
     1561 gaataacata gacatgcgat ccacgaatac tttcttcaat gtttacttga atttctccat
     1621 cactaaattg agtaacagat gattttccta actcgacgcc gactgctgct gcaattttct
     1681 ctgctaatgg gcgattggaa tttaacgcaa aaatttttaa tcttgggtca aagtaatgtt
     1741 ttgacatggg gacctccact ttctttttca caactctaga attctaccta ataaatagta
     1801 gtcatatttt ttaaataaat caagtgattt tgcaaaagtc ttagtttaga taagggagtt
     1861 tttttgcata tccttcttta ttgacttgtc ttgctctcgc aattgccagc gcatattctg
     1921 gaatattgtc tgtaattgtt gacccggcag caatcgaact gtttttagct acctcgacag
     1981 gtccgatgat attcgtgcta gaaccaataa agctgtggtc gccgacagtc gtatgatgtt
     2041 tgttctttcc atcatagttg acgaagacaa caccacatcc gacattgatt tcttctccca
     2101 gcgtagcatc accaacataa gtcaagtgac cgactttagt tcctttgccg atttgagcat
     2161 ttttcacttc cacaaagttt ccgatatgga cgttttcgcc aatttcagct tttggtcgta
     2221 aatgtgcata aggtcccaca tctgcatgtt tcttgacatg gctgctttca atgactgagt
     2281 tttcaatgac gacgtgatcc tcaatcctac tgtctacgat ttttgaatgt gacccgatga
     2341 cacaatcaga accaatgact gtatttcctt ggatttgtac acctgcttcg atcaatgtgt
     2401 ctggaccgat ttctacgcct gcatcgatgt atgtcgttgc tgaatcgaca aagcttacgc
     2461 cattgaccat atgcatctgg ttaattcgct ttctcatgat ctcatttgct ttagctaacg
     2521 cgatccgatc attgactccc attgattctt caaaatcatc cgtttgataa gcagcaactg
     2581 tatggccttc ttcttttaag atttcgatga tatctgttag atagtattca ccttgagcat
     2641 tgtttgttcc aactttgctt aacgcatcga acaaagcttc gttgtcaaaa cagtaagttc
     2701 ctgtattgat ttcttgtaca agagcttctt ctggtgttgc atctttttgt tccacgattt
     2761 tttctacgat cccaatatgg tcacggatga ttcgtccata tcctgttggg ttttctgctt
     2821 gtgccgtcaa gatcgttgca cttgcatttt ttccttggtg atattcaaaa agattgttca
     2881 gcgtttcagt agtcaataat ggtgtatcgc cactgataac taacgttgtt cctttttttc
     2941 cttccaagaa agaagcagct tgcaaaacag catgtcctgt tcccaattgt tctgcttgta
     3001 acgcatattt actgcgttct cctagttgtg ctttaacttg ttctgctccg tgtccaacga
     3061 tcgtaatcac ttcatctggt ttcgtttcac tgacccgatt aatgatatgt tctaccatag
     3121 gtttaccaga aacgggatgt aaaactttat ataattttga cttcatgcgg gtacctttcc
     3181 ctgccgccaa aataattgca taacgtgcgt ccaactgcgt cactcctaaa tatagtttgc
     3241 tgatttcagt ttatcccagt atacccattt tttcaacaaa acaagtgatt ttcttttatc
     3301 ccagcgtttt ctggataaaa aaatagatag accataacgg gcctatctat ttggtaaccg
     3361 gtgctctcct gccaactcct cggtcacaag tcacactact cataaaacat gaaaagctgt
     3421 tttttgaaat cgtttcttgt gacttagagt taaacggtag gcttacaacc tcttcattaa
     3481 acgtttttca atcagctgac cttcggtatt agctcaataa acggcaaaat atgaggagca
     3541 atttttccgt ttattgagct aatactcagg acaggacgct gatttcacaa cctctagata
     3601 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgaaaaa atgtggaaag
     3661 ccattttcac aaaatttttc ttactactcg aaactaagca gctttttcac aacctcttat
     3721 tctgaaaaat aatttcctgg cacaaccgta atatttttcg tttgagtatc tacatctttg
     3781 acataaagca gtgacgtata atcatcgatg gcgcgatgac cgttgaattt tgattcggca
     3841 aatacagtga tgccaacaag ttctgattcg aattcttcaa tcaagctctt cattccgttg
     3901 actgttccgc cgcctttcat gaaatcatcc actacaagga cacgtgaacc acgtttcaaa
     3961 cttctcttag acagttccat cttttcaatt ctttcagaag atccagacac atagttgaca
     4021 cttactgttg atccttctgt tatttttgag tcccggcgta caataacgaa tggcgcattc
     4081 agataatagg acacagcttg ggcaataggt acgccttttg tggctacagt cattacagca
     4141 tcgatttttt caccaaggta tttagaagca atgattcttc caacttgacg cagcagttca
     4201 ggttctccta acaaatcaga gagataaaca tatcctcctg gaagcagacg gtcttgttct
     4261 gatagacgtt ctgctagaga ttcaatatat tttttcgcct cttcatatga aatttcaggg
     4321 atgaacaata caccacctgc agcacccggg attgtttcca aggttccata ccctcgttct
     4381 ttaaatgttt tcttgatgat tcccaaatct tcactgatag aagatttagc agattcataa
     4441 cgttcggcaa agcttgttaa ggaaatcaat tcatgcgggt gattcaataa atactgtgtc
     4501 atgtcaacta gacgttcact gcgacgtact ttcacctatt ccaactcctt ttaagaaatt
     4561 ttatcgtttt acattatata agtttttctt caaaaattcg agcattttat gaaaaaataa
     4621 gaaaatagtt cggtatttat taaaataaaa agaagcctat aaaaaatttt tcgtttttat
     4681 aaggcttctt ttctttataa tttagttatt ttttcttctg aaggcaacca atattcgttt
     4741 gatgatattt acaatcagga acaaagcaat aaagactaat gtgattgttg ctcctggagg
     4801 tgtatctaga taaaacgagc tagttagtcc tgacagcatc cctagaaatc ctacacaaac
     4861 actgatagca atcacgacat tgaagctttt gcctaatcgc ataccgattg ctgcgggaag
     4921 aaccatgatt gcggatatca aaagtgcacc ggcaatcggg atcatcacag caatagccac
     4981 accagtaatc acattgaaca tcatcgacat caaatgaacg ggtagcccat ccacgtgggc
     5041 ggtatcttca tcaaaagtca gaacatacat cggccgtttg aataaaaaga acaatatacc
     5101 gatggcaacg aataagattc ctaaaaagat gacttgctcc catgtaatgg tcacgatcga
     5161 accaaacaga taggattgga tacttgttgc tgaattccct ccgctcaaat tcatcaatac
     5221 taacgctagc gccaaaccac cagccattaa aatggcaatg gagatttctg aataggttcg
     5281 gtagatcgtt cgcagatact ccaatatcac agctgctaat acaacgacta gcatggtcgt
     5341 aagtgtagga ttaaggttca aaaagaatcc taacgctacc cctgctaaag agacatgaga
     5401 aagcgtatca gccatcagcg attgtcttct gatcactaaa aacacaccta gcatcggggc
     5461 gattccggca atgaagatag cggcaagaaa cgctcttctc atgaattcat atgaaagcaa
     5521 cgccattgag aatcctcctt tctcaccagt cggatctgtc tgtccgcata ctgtttgata
     5581 tcttcatgat catgggtgat cattagaact gatttcccat ggacatgcgc actatgtcgg
     5641 agaagacgat aaaattcatt tctggattct tcatccatac ctgttgttgg ttcatcaaga
     5701 acaaataagt cagggtcagt agcaaaaata cgcgctaaac tgattcgctg cttttgtccg
     5761 ccagacaatt cacctattcg ccgatttcgc atatcccaca tccctacagc ttctaaagca
     5821 cgctgtacat gttgatgatc tttttcagag agacgcttga accagcggcc tcttggatat
     5881 cttcctgaac ggactaattc gatcaccgta ctaggaaaac ctgcattgaa agatgcaact
     5941 tgttgaggaa tataaccgat acttaatttt tttccttctt gatttttctc agcaatcttt
     6001 atcattcctt ttgaaggttt cagtaatcct aacgatgctt tcaccaaggt tgacttggct
     6061 gctccatttt ctccagttaa aatgacaaat tcacccgcat ctacatgata agagacatct
     6121 tctagtacag gttcatcatc atagtaaaag gtcaaatcag atatttcgat ataacgcacc
     6181 ttacaacctc cattctaaaa cttaattgat actttctttt aaagctttca gatttttttc
     6241 catgacagaa atataatttt cgcctgcttt gatttgttca ttagtcaagc tttctaaagg
     6301 attcaacact tctagcttca ctcctgtttc attcgacaat gtctcagcta ccttcgaaga
     6361 ggcattttct tcaaaataaa tcactttgat gttgtttttc ttcacaaatt cttttagctc
     6421 agctaagcga cttggtgtgg gttcctgatc tggtgaaacg cccgaaatcg ctacttgttt
     6481 taagtcgtat tgattcgcta aataagcaaa agcagcatgc tgagtgacaa atgtgcggtt
     6541 tgttgcatct tttagctcat cggagtattt ttgatccagt gcttcaagct ttttgatata
     6601 agcatcggca ttcttcgtaa aagtttcctt tttatctgga tatttttcgc ctaattcttc
     6661 agcaatcgtt ctgacttctt ttatagctaa taccggatcg agccaaacat gtggatcgta
     6721 ttcatgtgaa tgaccatgcc cttcttctga ttcttcatgt tcgtctgctt cttgcgattc
     6781 taaacggtcg attcctttag ctgcttcgat catcaaggtc tgtttgctat ccactgaatc
     6841 ctttaacgaa tccacaaaaa attccatgtc tgaactatta tacacgaaaa catctgcatc
     6901 ggaaatttct gctctttctt ttgcacttgg ttcgaaatca tgcggctccg ttccagcagg
     6961 gatcaaaagt ttcacgtttc cttcatcccc tacgatctct tttgtaaaat catacatcgg
     7021 ataaaaagtc gtaacgatcg tcaaatcctc ttttttgtct gcttccttgt tcgtatttcc
     7081 acatgctgca aacagtaaag cacctatcat taccgtagct gtcaaaagat attttttcat
     7141 ttatatgctc ctttcaatga acaaatcgga attttacgat ttattaacgt gaactaatgt
     7201 atcaaaataa aaaaacacag tcaatagaaa tccatcaatt tctgatttaa gcatgtatat
     7261 tcttgattaa atcgtaaaat ggatgagttg ctgctcatat ctagccattt cactacaata
     7321 aaaaactgaa gaataccatt ttaggattcc tcagtttttc atcacctatt ttacttctta
     7381 tattttattg cagggttcgt actaaataaa cttcctcaca aaaacctttt aacccattat
     7441 atactctctg cgctcttgaa taatgttgac acaaggcaaa aacggttggt ccgcttccac
     7501 tcatcaatgc agcatctgct ccgtacttga tcatacgctc tttgatttga tggatgactg
     7561 gatgccgctt tcccgttata tcttctaaac tgtttcccat ttttttgatc atctgctgat
     7621 aatcttgctc tcggatggct tcagcaagtc cgtggatgtc ttgatgttgg atggcttgcg
     7681 tatctacttc tctaaacact gtacgtgtag agacactcat tcttggtttg accaacacaa
     7741 cccagcactg tggcatagaa ggtaatactt ggatttcttc ccctttgcca ccgacaaatg
     7801 ccgtacttcc gtagacacag taaggaacat ctgtaccgac ttccaaaccg atttctgcca
     7861 gttcttctaa cgaacagccc agattccata attggttgat ccctcttaga gcagctgcac
     7921 agtctgtact tcctccgcct aatccagctg ctactggaat attcttctta acaaagatct
     7981 tgacaccggt tttgatattg tatttatttt ttagcaataa agcagcttga taaacattgt
     8041 tccgttcatc taccggtaaa aatgctttat ttgtttctac aatgattttg tcttctgtta
     8101 tttcttcaaa aaatagatga tctgccaagt ctacgctcga catcaccatt tccaactcat
     8161 gatacccatc ttgccgtcta tataatacat ccagtcctaa atttatcttg gctggcgctt
     8221 tttcaatcaa ttccatcctc tcacccattc gaaaagtcta agtcgcaagt attctatcat
     8281 agggaaaaat aaaagtatag cttatgtctg cctagtggtt gattttgtca tcgtttcaac
     8341 agctttcaca gaattttctc ttcataatcc aaacaagttc tagtgattct tataattctg
     8401 tgagcacttc ttctgccaaa ctcggaacat ttcctttttc ttttcctatc agactgagta
     8461 gctgcatggt agcaaatccg aaattacttc ctttcctata agcaaaataa cgtccttccc
     8521 aatgatcgac atattttaat ttcttttcat aagcagactg gaaggaatag aaaaaattac
     8581 cataattata tacgatatgc atgacttttt cttgaaagaa agaaaaatct gtttctccaa
     8641 cattagctaa tggagctaag cctagattgg cttccgagta gcccattttc tcgtatacaa
     8701 taaacagatt ggctaacaga tagtcagcta attcctcaga ttctttaggt aagacccgca
     8761 acaaatcata tcccgcctgc ttcttcgtca tcggctgagc ggtgatgaag ccaacgactt
     8821 tttgatcttt acggacaata ccgacaccgc tagataatac atatgattta tcaaatcgtc
     8881 ccacagagaa gtatttttct cgtgcacctt gaagccattt ttgtgaaact tcatttacag
     8941 cttgatgtaa ttcttccggc agttgatcgt accaagtaaa atggtatcct tctttcatca
     9001 aacgttgata ttcaagttgc tgactcagcc gagacaagtc ttctgttgct agatcaacaa
     9061 tccctgcttc acctactttc gtgaagtgaa aacccaattc atgaagcatc actacgtagc
     9121 tttctgatat cttataaaac gcaagctgat agcctaaaca atctgcttgt ttcataaact
     9181 gattcgttgc tgcccgcatc attttaggat tgccaactgg atctcctaaa acaaagcatt
     9241 tgtttccttt gatctgataa ccaaaaacga cttgatcttg gtcgtcttct tggtaataaa
     9301 aataactgta acctgacaag cgaagctgat ggcttgtagc tgtgcctccg tatgtttcca
     9361 ataaacggtc aaaacggtct ttttcgtact cactgccaag cttcgtagta tcttcagcta
     9421 aatattgata aagcgccact aaagctagca atgagatgcc caaaccaata agccctgaga
     9481 accacacatc atctgaagga aacaaaataa aatgatttcc tgttattcga ccgactaaaa
     9541 agcgtccgct gtgatagcct gcaattgcgt aaacgataaa caaaaagccg aacaagctag
     9601 catcaaagat tatagctccc caagaataaa cgaattttgt tcgataaaat tcttttctgg
     9661 ataaccacac agctaacaga atcaagacat ataccaatat caactgccaa gataacgtac
     9721 gtgtgattgt atttaaaata ccaaaagata gtaaagcaat cgtaggcaga aatgcctttt
     9781 tgacttttac tgaaagtgct ctagcaaggc cgagtaataa aaagccaacc aatagattca
     9841 ctgtctgatc taaaaaattg aatgagaacg gcaataatac ttgaaatagc cgactgattg
     9901 cagacagatt agtgattgtt gatagaagta ccatcatgat ccccgcaaaa tagacagcac
     9961 ttacgagcac gatatgcgca accttttgga aaaacagtcg cggaatgtta tctaaaaatc
    10021 gattgatttt gactcctgta tgtgtgatga acaatagaag tcccgtcata aaaggtagaa
    10081 cataataaaa caaccgataa tataatagcc aaactactgc tacatcttgc cgaacaggca
    10141 attgactcat tcctaagatc atcaaaacgt caaaagtccc cattccacct gggaccatcg
    10201 tcagcattcc tatcaatgta gcaatcatga acataggata gatcgaaagc atcgaaatat
    10261 ctattctcat caacttgccg atgatcaaaa ataccgcaag ggctcccgtc cattgtccaa
    10321 aggatgccag tagcagacca agcaagcctt ttggataaaa ctcgaagaac aatgtctttc
    10381 tctttagata agcaaaaaag aaaatgactg gtgctaaaaa actaccgccg aaaagccaga
    10441 tccaatagct tcgaaaaata ctttctgact gaataaaaaa gacatcaata aatgtaagaa
    10501 acgaccatac agacaaacca gtcagcataa atagtgcgac ttttgataca gtggctaaaa
    10561 ccatttttct atttgtctgc tttccataaa aactagcccg cagacttgct cctaccacac
    10621 caccaaaacc ggcgagattg ttgatcgtgt ttgttaccca cgcagcaaga agaaactctt
    10681 ttctttccat ttttggtttt ccttgttttt ctaagactct gatcgtcacc caatcataca
    10741 acaacatcgg agtgacaccc agcagtccag ccagaatcat gagcaggatc gtcatgcggc
    10801 tttgttgtcc catcctttgc caaatatctt gccaactcat gccattagca atatttgcga
    10861 cttgatttgc cacaaagact aacacggagc cgaaaaaaat cagttttaaa atcagactat
    10921 gttcctttat ccattgcagc atcaagtatc gtttattttt cacattttcc cattcctaac
    10981 ttgtatactt ttgtgtcata ccccaatgtt atcttattta ttttaatttt tccactttag
    11041 acctgttctt tgcttgcgat gatagaaaaa gaaagatatc ctaatgctaa aggagaggat
    11101 aacatgaaag taggatttat cggcacaggg aatatggcac aagctattat tgatggttta
    11161 ttaaaaaaag gaacagtctc gaaagaagag gtgttcgttt ccagtggaca ttttgaaaat
    11221 gcacaaaaat ttgctgctga aactggcgtc catgcatgca aaagtaatca ggaagttgca
    11281 tcacaatcag atatcgttct tttagccgtg aaaccaaaaa tcattgaaaa agtattggaa
    11341 gaactaagag aatatagcca gaaagtctta ttcatttcta ttgcttcagg aaaatcatta
    11401 acagatttag aaacagcgct tgaagatcct caggcctcca tcgtacgtgt gatgccgaat
    11461 gtcaatgtca gcgtaggagc aggcgtttct gcgatttgca aaaatgaagc tgccacggag
    11521 gaacagcttc agcaagctac gatgattttt gaagcaatcg gcagcgtcta tcctttagca
    11581 gaatcagatt tcagtacatt cattggcctt gctggcagct cacccgcatt cgtttacatg
    11641 ctgatcgatg cgatgggacg gactggtgta ctgaatggtt tgtccaaaca gacagccact
    11701 gagattgctg caaaagcaat tatgggttct tgtcaaaaat tcctttcaag tgataaaaat
    11761 ccatgggaac ttgtagacca agtttcttct cctggcggta caactgttgc aggtgtagtt
    11821 gccttagaag aagcaggatt catcccagca atcatcaaag gaatcaatac gacgatcgag
    11881 aaagatcttg agatgttgaa taaaaaataa tgatagcaat gttttttcgt gccttacaaa
    11941 aaaagaagag ctgtggcttt tgccgcggct cttcttttgt attttttatt atttagcata
    12001 atcagttgca cgagtttccc gaataacgat tactttgatg tgtccaggat aatccagttc
    12061 ttcttcgatt cgtttacgaa tatcacgtac taaacgaaca gcgtctaaat ccgagatttc
    12121 ttctggcttg accattaccc gaacttcgcg tccagcttga actgcgtaac ttgattctac
    12181 accggcgaag ctgttcgaaa tattttccag acttcttaaa cgacggatgt agttttctaa
    12241 tgattcactt cgcgcaccag gtcttgctgc tgagagcgca tctgctgcag ctacaaggac
    12301 agagataaca gaagtagctt ccacatctcc atgatgagaa gcgatcgcgt tgattactac
    12361 tgggttttct ttgtacttgg cagctaattc cgcaccgatc tcaacgtgag atccttcaat
    12421 ctcatggtca agtgctttcc caatatcatg gagcaacccg gcacgttttg ctaattggat
    12481 atcttcgcca agctctgctg ctaagatacc agctaatttt gcaacctcga ccgaatgctt
    12541 caagacgttt tgtccataac tagttctgaa tcgcagacgt ccgatgatct tgattagatc
    12601 tggatgaagt gtatgagcac cgacttcaaa cgctgcctct tccccgtatt cgcgaatacg
    12661 ttcatccatt tctttacgag atttctcgac catttcttcg atacgcgctg gatggattcg
    12721 tccatcttgt atcagttttt ccaaggtcat ccgtgcgatt tctcttcgga ttggatcgaa
    12781 tccggaaaga acaactgctt ccggtgtatc atcgatgatt aaatcgatac cagtcagcgt
    12841 ctcaagcgtt cgaatattac gaccttctcg tccaatgata cgacctttca tttcatcatt
    12901 cggaagggta acgactgaaa cagtcgtttc tgaaacttga tctgctgcgc agcgttgaat
    12961 cgctaatgat aataagtttt tcgctttgcg atctgcttct tctttggcac gctgttcaga
    13021 ttcttttacc attagagtta actcatgatt cagctcttct tcagtcgatt tcatgatgac
    13081 atctcgtgcg tcctcttttg ataaaccagc aattttttct agttcttttt gttgatcttc
    13141 aatcattttt tcaacgtctt tttctcgctc ttcaattaat tgctgttttg aactaagtgc
    13201 ttcttcttta ctttcaagtg agttctcgcg tttttctaac gagtcatctt tacgattcag
    13261 cgtttgttcg cgttgaatca aacgattctc ttgagatttt aattctaact tactttctct
    13321 cagctcactt tcaacttcag aacggtattt ctgattttct tccttagctt ctaacaatgc
    13381 ttcttttttt agagtttctg cttctttttt tgcactatcg atgattccag cagctgatga
    13441 ttgtgcacca gctatttctt tctcgtgacg tgactttgcg accataaacc ctaaaccaag
    13501 accgacaatt aaaccgatga tagcgaggag aatgttgaat accataaaat ccacctccat
    13561 actatctttt tcatttttat catctataca gcttctatga taaattattc taaactcgtc
    13621 aatatgcatc ttcgcatata tgtgtacaaa aaacatgcac aactttattc taatgtttgt
    13681 accccgtagt gtcaacttta aataaacaaa gaaacggctt tctacttatt cattaaacga
    13741 tggtttttaa aataaatgac actagtttca tataggtaaa cctttaaatt gagaaacttt
    13801 tctattaaat tattctttgt agatgaacag atatttttat tttatgtacc cactcgtata
    13861 attaaataac tcacgatttc tttattcaat aaaaaaacgg caggtcaagg agagtaccta
    13921 ctcttcagac cagccgtttt tgtttatcat aatttttatt cttctactaa aggcaattct
    13981 tcttgtcctt tttcatcttc tgggacagcg acttcttcac caataccata agctgctcta
    14041 actagagcag aaacttctgc catcatttca ggatgattag ccatatagat tttagcgttt
    14101 tcccgtcctt gaccaatccg atcttcttta taagaatacc aagcaccgct tttatcaaca
    14161 atgtcttttt ctactgccat gtctagaagt tctccttctt gtgagatccc taaaccatac
    14221 atgacatcta cttcagcgat tttgaaagga ggtgctactt tgttttttac gactttgatc
    14281 tttgtacggt taccaacaat atctgtacct tgtttcaatt gttctgcacg acgtacttcc
    14341 aaacggatcg ttgcgtagaa tttcaatgcg cgtcctccag gagtgatttc tggattccca
    14401 aacatcacgc ctactttttc acggatttgg ttgatgaaaa tagcgattgt ttttgttttg
    14461 ttgatcgaac cagagagttt acgcaatgct tgagacatca aacgtgcttg taacccgaca
    14521 tgtgagtcac ccatttcgcc gtcgatttct gctcgtggaa ctaaagcagc aactgaatcg
    14581 actactacga tatctacagc cccacttgat actaaagcat cagcgatctc tagaccttgt
    14641 tctcctgtgt caggctgtga aagaagtagt tcatcgatat tcacacctaa tttttgtgca
    14701 tattgcggat ctaacgcatg ctcagcatca atgaaagcgg ccgttccgcc atttttttgt
    14761 acttctgcaa tagcgtgtag tgcaactgtt gttttacctg aactttcagg tccatatact
    14821 tcgatgatac gtccacgggg gtaaccgccg acacctaaag cgacatctaa cgcaagagag
    14881 ccacttggaa ttgtagagat ttgttggtcg attttttcgc ccaatttcat aatagagcct
    14941 ttaccatagc tcttttcaat ttttttcagt gcagcatcta aggctgcttt acgatcatct
    15001 gccaatacga taatcctcct tatatttaca aatcagagca acatttcatt atccttatca
    15061 tatctttttt agtgaaaaaa agcaagaaaa aaccgaacaa atattcgcat ttatcatttt
    15121 cttaaaagct gttttctcaa aagatcaaat ccttgcataa ccgcattata tctgatagca
    15181 gcacgatctc tggcaaagtg atatttttcc gaaaccacgc cgtctttaga tgaaatagcg
    15241 atccatactg ttcctgcaga ttgaccttct aacgtgtccg gtccagccac tccggtgaaa
    15301 gctacactgt aatctgttct tgctatctca cgtgcttgaa tggccatctg ctccacacat
    15361 tctttgctga ctgttccata ttctgctaat agctccggat caatctccag aaaaccagct
    15421 tttgtttgga gcgagtaggt aacgaatccg cctggaaata cttcagatac accagcaatg
    15481 tcgcctaatg cggcttggaa ggcgccggct gtcaaacttt ccgctgctgt tacagtctgc
    15541 ttgttttctt tcagtagttc cactacgact tttgctagcg aattatcatc gccgtatcca
    15601 taaaaatatt cccctactcg ttcttggatt ttttcttcta atgctaaaag agcttgattc
    15661 cctgcctgaa catcatttgt tttcactgtc agtcgtaatg tcacttcatt tggtttggca
    15721 tatggtgcga tcgttggatt gatctgtgtc tcaatcagat cttttagctc agtgaccagt
    15781 cgcgattcac caatcccata aaagcgcaaa actttagaaa ttagtttttc ttcagaagga
    15841 aaacgttcct ctagtaaagg acgtacttgt tccacaaaca ttggtttcaa ctcattaggc
    15901 ggaccgggaa gaaggatata cgcatgtgta tctgtttgat aaaaagtccc taaagcaagc
    15961 cctgtacgat ttggtagagg aaccccgcct tcgataatct gggactgttg cagattgttt
    16021 ttggtcattt ttcgatgggt tgtttcaaaa tatgccaata attttttgta tccttccgta
    16081 ttttgtatga gagacttacc gaggtgagcc gcagttgctt cttttgtcag gtcatcttct
    16141 gtcggtccta atccgccaca taagagtatg agttcacttc ttgtttcagc tagagcaatc
    16201 aactcttcta aacgctgctg attatctcct acaactgttt gataataaac ttcgatacct
    16261 agatcagcta gctgttctga caagaaagtg gcattcgtat tcacgacttg tcctagtaaa
    16321 agttctgttc cgacagctat tatttctgct ttcattactt cccgctccct tatatagtat
    16381 acgctgtatt tttatatttt tttatttaaa aagagggcaa ggagctgtga caaaaatctt
    16441 gtcacagctc cttgttccaa ataaacagtg ttctcctgcc aaaacctcgg tcataagtca
    16501 caatatacat aaaacatgaa aagctgtttt ttgaaattat cccttgtgac tcagagttag
    16561 acggcaggct cacaacctct ccactaaacg atacttacat tgatcctttg aacacaccgc
    16621 tgtttttgat gaagtaatcc acacctgaat agatagtaaa gatcagacaa gcataaagca
    16681 tgatttgatc tactggaata ccgatcaggc tgaatgggat attattgata aacagcaaga
    16741 taatagcaac catttgtgta gccgtcttga cttttcccgg ccaagctgca gccatgactt
    16801 ctccatgttc gacaagtagt aaacgcaagc ctgtgacggc taattcacga cagacaatga
    16861 tcgctactac ccaaccaggg gcctttcctt ggtcaaccaa catgataaaa gcagtcataa
    16921 ctagcatttt atctgccaac gggtccgcaa acttgccaaa attagtgaca aggcctcgcg
    16981 cacgggcaat ttttccatct aaccagtctg tgatactagc taaagcaaaa ataactgcac
    17041 ctactagctg tgtgacagcc agtgtggtgt ctcctacgtt aaattgtccc caatctaaag
    17101 gtaaacctac aaccaaaatg aaaatcggga tcatacaaat tctaagtaca gttaatttat
    17161 ttggtaaatt cacactatcg actcctttct tccctattct acgtgactat cagcgaagtt
    17221 tccaccgata ttatttactt ttccaaatga atttattcaa aggagtattt tt
//
LOCUS       DAFEHS010000057        17005 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFEHS010000057
VERSION     DAFEHS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.059"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..124)
                     /locus_tag="LP327_001956"
     CDS             complement(<1..124)
                     /locus_tag="LP327_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBL1726063.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="LP327_001957"
     CDS             complement(228..539)
                     /locus_tag="LP327_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL1726064.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="LP327_001958"
     CDS             complement(541..738)
                     /locus_tag="LP327_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBL1726065.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="LP327_001959"
     CDS             complement(1132..2859)
                     /locus_tag="LP327_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBL1726066.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="LP327_001960"
     CDS             complement(2852..4045)
                     /locus_tag="LP327_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL1726067.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="LP327_001961"
     CDS             complement(4232..4876)
                     /locus_tag="LP327_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL1726068.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="LP327_001962"
     CDS             complement(4970..5104)
                     /locus_tag="LP327_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBL1726069.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="LP327_001963"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="LP327_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBL1726070.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="LP327_001964"
     CDS             complement(7235..7708)
                     /locus_tag="LP327_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBL1726071.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="LP327_001965"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="LP327_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBL1726072.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="LP327_001966"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="LP327_001966"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBL1726073.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="LP327_001967"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="LP327_001967"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBL1726074.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="LP327_001968"
     CDS             complement(11755..12894)
                     /locus_tag="LP327_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL1726075.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="LP327_001969"
     CDS             complement(13203..13916)
                     /locus_tag="LP327_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBL1726076.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="LP327_001970"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="LP327_001970"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBL1726077.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="LP327_001971"
     CDS             15105..16637
                     /locus_tag="LP327_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL1726078.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="LP327_001972"
     CDS             complement(16828..>17005)
                     /locus_tag="LP327_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL1726079.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DAFEHS010000058        16904 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFEHS010000058
VERSION     DAFEHS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.060"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..190)
                     /locus_tag="LP327_001973"
     CDS             complement(<1..190)
                     /locus_tag="LP327_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726080.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="LP327_001974"
     CDS             complement(200..1015)
                     /locus_tag="LP327_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL1726081.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="LP327_001975"
     CDS             complement(1277..1720)
                     /locus_tag="LP327_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBL1726082.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="LP327_001976"
     CDS             complement(1854..2192)
                     /locus_tag="LP327_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL1726083.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="LP327_001977"
     CDS             complement(2180..2557)
                     /locus_tag="LP327_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL1726084.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="LP327_001978"
     CDS             2781..3974
                     /locus_tag="LP327_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL1726085.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="LP327_001979"
     CDS             complement(4140..4562)
                     /locus_tag="LP327_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726086.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="LP327_001980"
     CDS             4957..5151
                     /locus_tag="LP327_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726087.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5642
                     /locus_tag="LP327_001981"
     CDS             5148..5642
                     /locus_tag="LP327_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726088.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(5804..6976)
                     /locus_tag="LP327_001982"
     CDS             complement(5804..6976)
                     /locus_tag="LP327_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL1726089.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="LP327_001983"
     CDS             complement(7182..7997)
                     /locus_tag="LP327_001983"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL1726090.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNMI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="LP327_001984"
     CDS             complement(8147..8593)
                     /locus_tag="LP327_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL1726091.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9594)
                     /locus_tag="LP327_001985"
     CDS             complement(8590..9594)
                     /locus_tag="LP327_001985"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL1726092.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9634..9963)
                     /locus_tag="LP327_001986"
     CDS             complement(9634..9963)
                     /locus_tag="LP327_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL1726093.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10089..12416)
                     /locus_tag="LP327_001987"
     CDS             complement(10089..12416)
                     /locus_tag="LP327_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL1726094.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(13259..13552)
                     /locus_tag="LP327_001988"
     CDS             complement(13259..13552)
                     /locus_tag="LP327_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBL1726095.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(13955..15133)
                     /locus_tag="LP327_001989"
     CDS             complement(13955..15133)
                     /locus_tag="LP327_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL1726096.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(15237..15551)
                     /locus_tag="LP327_001990"
     CDS             complement(15237..15551)
                     /locus_tag="LP327_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726097.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            15658..15873
                     /locus_tag="LP327_001991"
     CDS             15658..15873
                     /locus_tag="LP327_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726098.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(16173..16415)
                     /locus_tag="LP327_001992"
     CDS             complement(16173..16415)
                     /locus_tag="LP327_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726099.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(16402..16830)
                     /locus_tag="LP327_001993"
     CDS             complement(16402..16830)
                     /locus_tag="LP327_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL1726100.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFLI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         4927 a         3271 c         2936 g         5770 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag cgaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcatgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctcaata aaaaatcatc aaataatcct ttacacgtta ttctaaattt aatttttatt
    12481 attttattga tgtatagaaa gtttggggct ttttttctct ttttattcta aggttatttt
    12541 agtcttgtcc tttaatacaa agattttatt tttaaataaa aaatactctt tttttacgat
    12601 tattgaaaaa aaaagataaa acttgattaa ttgaagtatt gcttaaattt ggaagctatg
    12661 agggaagaat aaaagagtta ggacgggatt atctactatt tttctttgtt ttaaatattt
    12721 gttatctttg aacataaaag agaaatgtgt cgactgcagt gttgtgtccc tcatcacgaa
    12781 taaacggtgt tcaattagct gatcttgtat tcacttaaaa aatggtaaag tacgagttgc
    12841 tattccccat tttcaatttt ctaacgctca ggacaaaata ctgttttcac aacctcattg
    12901 gaataaaagc atatatattg tttcaagaac gaaatcttgt tatcttttta aagggcatct
    12961 gtccaaaatc taattttagg aggtacgttt tggtatttat tcgtacaaat aaaagagaat
    13021 tatctttatt catgtaacgc atccaattga ttttttcatg tacgctcctg gaacgatttt
    13081 actgacgatt gcttatttaa ctgcgaatcg ctcattagct tttatcatag ctatccatat
    13141 tttgaataac gttcttggct ttgtattata gatacagatg acaaaaaaag ttcaagtatg
    13201 cggtgtagaa ccaatatcgc atacttgaac ttttttcaat gagtataaat taaaacttct
    13261 aattttcaat tcccatcatc tgatatgccc gaccgtctga acttcttttg aaaaagccaa
    13321 atgtgattag ataacgtctt tgttcgacat aatcgccact agaaaatacg ttcagcagta
    13381 tcgaattaac ctctgtttct gtataagtca ctccatggtc aaagcgtttc aagacttctt
    13441 tcaataggat gtatttcttt tttgttgaac ttggtacttg ttttactttt ccatcattca
    13501 tgaatgtgtc taaaactttc atcttttctt gctccgtata agtataaacc atcttctttc
    13561 tccttttgat tctaagaata ctccatttat gttagaatat tctggatttt ttattaaagc
    13621 ggttactctt aagaaagata ttatcatagt gctctttttt ttactactac ttataggtag
    13681 atttattcta atccattatt ctcccctacc aataattaaa gaaaaaactg gatcagggtg
    13741 ttcaaattac atgctaagaa aaaagaaccc ttgaatacca agggttcttt ctcagtctga
    13801 taacaatgat taacgacgga tttctttgat acttgtgaaa agtcgttaaa gtattgatat
    13861 aaaaggattt ttattacctc tgactacata ttgactaatt tgttaaaaaa tcgtttttca
    13921 agaatcctgt aataccagtg ctttcaccgt ttttttaact ctctaaagaa ctaaaagcaa
    13981 tcagaggttt taccttttca tttgacgtta agtggcgata aattttcgaa gttgtttggt
    14041 cagtatggcc taagcgatct ttcaagactt cccagctaca gtgttccgct actaagcttg
    14101 ccattgtatg acgagctaaa tgagtatgaa atggcttatt aattcctgag cgattcacgc
    14161 accgtttgac gtgatcattt acatactctc gacgataagg aacaccacgt ttactagaat
    14221 caagatacac aaacaaatat gtttcttcta taataaacct gtaatgttgc ctcatctcac
    14281 ccaaagcctt aatcaactgt tgtagctttt ccataactag aggagttact agaactcgtc
    14341 tagcagaact aactgtctta gtagtttcaa ttcgaaaatc cttactctta cctttaactg
    14401 ccgtttgctt atagaacaga atttcatttc ttttgaaatc tatatctcca ggctgtagag
    14461 caagcgcctc gctgactcgg cagccagtgt aaaatagaac atggaaaagt acagacatcg
    14521 gtaattcctc atgaacaatg ccataattca aaaaagttct caattcatcg acagtaagaa
    14581 acttgtcctc aatattattt atggcttctt ttaaatcagt taccgttaac tgatattttg
    14641 gcattctcac agacttcatt ggattttcac ttatcaaatt ctttctttga caatatttaa
    14701 aaatcatatt tagaacttga ataagtgatt gtaaactatt tttggcatag ccaacatttg
    14761 ttccattaac gccacattcc tttaaggtaa aaagcaattc ttgaaggcac tctggtgtca
    14821 aatcagacaa ctttttatca ccaatccatc gaacaataat ttttaattgt tcctctcgct
    14881 tatcataggt tatttccttt tgttcattta acgaacggta atattggaac cattgctcac
    14941 ctacaacact aaacttgtca ttgcttgcaa caattttttt cttaagcctt tgttgtttct
    15001 ccatttccag ataagcgagt tgcgcctcac gttttgaatt aaagccacgt cttgttgttc
    15061 gatatgcttg aaagtaccat gtgatcctac cagtagaact gatttgcttc ttgaatcgat
    15121 ctgttttttc catttattga ttcctcctat tttgaaggaa cccaacaagc agaaaccctt
    15181 tggaaatctt tctatctatg tcatcattgt accgcatctt agaaagaaaa tgtaacttat
    15241 aaagatcgaa gtagtcgcaa tttttctcga tagtcattcg aatcactctt tctcatccat
    15301 tctaaaacat catcacgaaa atagtgtcct cgaatcccgt accaacaggg aggaaactca
    15361 ccaagtttta ctaaacggtt cagtgtacta tcacttatct gaaatagtcg ctttaaatca
    15421 gtatttgtta ataccagagg taaatgactg actgcaagca atttttccgt gtaatcttcc
    15481 agtttttttt gtaaaattga atagaactct atttccagag aattaacatt tgttttaact
    15541 tccaactcca tcacccttct ctctcaaaat caaaacgacc accacggtac cccttaaaat
    15601 tgtaaaaaaa cactccagta ctccatcaaa acggactaga aaagaggaat ttttaatatg
    15661 aaacaaggaa ttgcagacat aaaaataatt aaagaaattc ttgaaaaatc tacggctaat
    15721 gcaatagcat tcggaacagg aatcaacttg agtacagtaa aaaaacttaa aagtggagag
    15781 agagctgaag aaaaattaaa tcttgccgat gctattaaaa tcacagaatt tggcatgaaa
    15841 aatatgccaa ccaaaatcga aatatggaaa tagtagtttt tctgatctat cagctgtttt
    15901 gccaaagcac ataggaatct cacctccccc cagtaatggc gggccgattt ggttacggaa
    15961 gtatcattat cgtagtcgcg attctcatat taaaatttgt cgcttaaaag ctaaccttgt
    16021 catggcgtaa ctagagaatc aaaatctaca tgtactgata gatttgctat tcatttgtca
    16081 aagagcgcta ggctattttc agcctatgga aatcctttat tttgacccga taaaaaactt
    16141 ccatagaatg aaaaggaaag ggaaaaaata ctttaacgag ttgtatcaaa ttcaagtatc
    16201 ttctcaatca attttgtttc cagttgtcta cgcatatatt catctacata cataaccatt
    16261 cctttttctt cttcataaac tggtttcatg gctaaccgtg aaatataatt ttgatatttt
    16321 gacagaacga tggacatggc aattgaatca ccagaagctg cggctttgat tgtcgttagt
    16381 ggtatgaatg gtatatatcg tttattttgc ttcatatttg acacccaaat actttcctag
    16441 ctcttccaaa gccatttgtc gttggtacca aacactgccg agacttttcc caataatatc
    16501 gccaatttca cgatcattaa gaccgatgag gaaggacata aaaatgattt cttgtctttg
    16561 actaggtaat gctgcaatcg catttgccaa ctcctcattg gaaataaaga ttttcatacc
    16621 aaggacaata aaagtaccac tactattcaa atttggttcc aaataataga actctgtacc
    16681 taaccctttt tgttccaata tttccattga tatttgccgt tggcgaaatc tagcgtactt
    16741 cttttgaata tttaacgcct cattgcagac tactttctta caaaaactgt caaacgtatg
    16801 ttgaatgtgg gtttcctctg agtcgcccat aatattctcc ccctcaaaaa attcattcaa
    16861 ctcgttttac ttctatccct ttccattatt actacaatct agga
//
LOCUS       DAFEHS010000059        16844 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFEHS010000059
VERSION     DAFEHS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.061"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            163..1104
                     /locus_tag="LP327_001994"
     CDS             163..1104
                     /locus_tag="LP327_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBL1726101.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1173..1904
                     /locus_tag="LP327_001995"
     CDS             1173..1904
                     /locus_tag="LP327_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBL1726102.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2048..4228
                     /locus_tag="LP327_001996"
     CDS             2048..4228
                     /locus_tag="LP327_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBL1726103.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4248..4730
                     /locus_tag="LP327_001997"
     CDS             4248..4730
                     /locus_tag="LP327_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBL1726104.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4746..5507
                     /locus_tag="LP327_001998"
     CDS             4746..5507
                     /locus_tag="LP327_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL1726105.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5638..6972
                     /locus_tag="LP327_001999"
     CDS             5638..6972
                     /locus_tag="LP327_001999"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBL1726106.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7279..7764
                     /locus_tag="LP327_002000"
     CDS             7279..7764
                     /locus_tag="LP327_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBL1726107.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7757..8440
                     /locus_tag="LP327_002001"
     CDS             7757..8440
                     /locus_tag="LP327_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBL1726108.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8566..11211
                     /gene="polA"
                     /locus_tag="LP327_002002"
     CDS             8566..11211
                     /gene="polA"
                     /locus_tag="LP327_002002"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBL1726109.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11256..12092
                     /gene="mutM"
                     /locus_tag="LP327_002003"
     CDS             11256..12092
                     /gene="mutM"
                     /locus_tag="LP327_002003"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBL1726110.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12056..12685
                     /locus_tag="LP327_002004"
     CDS             12056..12685
                     /locus_tag="LP327_002004"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBL1726111.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12737..13069
                     /locus_tag="LP327_002005"
     CDS             12737..13069
                     /locus_tag="LP327_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL1726112.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13381..13872
                     /gene="nrdR"
                     /locus_tag="LP327_002006"
     CDS             13381..13872
                     /gene="nrdR"
                     /locus_tag="LP327_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBL1726113.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13895..15268
                     /locus_tag="LP327_002007"
     CDS             13895..15268
                     /locus_tag="LP327_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL1726114.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15268..16194
                     /gene="dnaI"
                     /locus_tag="LP327_002008"
     CDS             15268..16194
                     /gene="dnaI"
                     /locus_tag="LP327_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBL1726115.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16323..16763
                     /locus_tag="LP327_002009"
     CDS             16323..16763
                     /locus_tag="LP327_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL1726116.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5749 a         2949 c         3709 g         4437 t
ORIGIN      
        1 tttttctgtg cgaaacagga aagcgaagtc tttttaattc aaataataat attatgtaaa
       61 caaaaaactt tccatgcgta attatacaca agcaaataaa tcgtcaaatg cgaacgcctt
      121 ctttatactg tttaggaacg aaaagaagga aggaagaaat acatggatta tcaagtactg
      181 ctttattaca attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc
      241 ttttgcaaat ctttggatct taaaggtcgt attctagtag ctgaagaagg aataaatggt
      301 actgtgtctg gaaccgttga aaacacgact gcctatatga aagcaatgaa agctgatcct
      361 cgatttgctg atacgatgtt caagatcgat ccaagcgaag gacatgcctt taagaagatg
      421 tttgtaagat accgtccaga gcttgtaagt ttaaagctag aagaggacat tgatccaaac
      481 caagtgacag gtgcctatct ttcaccgcaa gaatttaaag aagctatttt agatgaacag
      541 acagtggtga ttgatgcacg gaatgattat gaatatgact tgggacattt tcgaggggct
      601 gtacgcccag atatacgcag ttttcgagag cttccagact ggatccgcga gcacaaagaa
      661 gaatttatgg aaaaacgggt agtgacctac tgtactggtg gcattcgttg cgagaagttt
      721 tctggttggc ttgtccgtga aggattcaaa gatgtcggac agctgcatgg cggtattgct
      781 acttatggta aggatcctga agtccaagga gaattatggg acggcaagat gtatgtgttt
      841 gatgaacgga tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg
      901 tttgacggaa caccatgtga acgatatatc aattgtagca atcctgagtg caatcgtcag
      961 attcttgctt ccattgaaaa tgaagaaaaa catttaggag catgtagcat agagtgtgcg
     1021 aaacatccgc gcaatcgcta tgttcaagac catggaatct ctccagaaga agcagaactg
     1081 cggtttacag ctttaattaa atagaaaaca atagttttga ttgtcgacca tttccaatat
     1141 gcgctatact agcgttgagg atggtgatcc acatgaaatc attgatttat gctattgggg
     1201 atgtccatgg caaacatgag ctttttgaaa aacttattca ctattatgat ccaaagatcc
     1261 atcagcttgt tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt
     1321 tattaggaaa gcaattagtc gaagaaacag gagccattta cctccgggga aaccatgaag
     1381 aatatttttt gcaatttctg caatcaccgg aggactggta tgcgccttat atgagaaatg
     1441 gtggaaaaga aacgtttgaa agcctgattc acccaggggc atcggaggaa tactccccaa
     1501 ctgaaatctc gatgatgatc caatcgagat attcagaact gattaaattt ttagtccagc
     1561 gccccttgta ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg gatttgacaa
     1621 agaaagactg gcgtaagaca agtctaagag actttatttg gataagggaa ccatttcatg
     1681 aagggaaaaa caatacaggt aagacaattg tatttggaca tacgattaca ccaatgctcc
     1741 atggagatat gcagacaaca gacctctgga ttcaggatca taaaatcgga atggacggtg
     1801 gggctgtatt cggagggtca ttgcatgggg ttgtttttga caaggaaggt attcttcaag
     1861 atattgaata tcagaacaac gaaggacctt ggcatggaat ctgactaatg gaaagaatga
     1921 aggagctgtg actttgttgc ggctcttttt atttttatag agaatattgt ttctttcagt
     1981 agtttaaccc aggataaaaa gtatgataga ataaagaagc aatttttagc agaaaagagg
     2041 cattttaatg acggaaaatt tgaaccagac gatcattcag cttgtacaaa aagaactttc
     2101 agactatcgt cctaagcagt tgactactgt acttaatttg ttaaatgaag gaaacactgt
     2161 tccttttatc gcgcgttacc gaaaagaaat gactggaagt cttgatgaag tccagattcg
     2221 tgaaatcgaa gagcggtatg cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat
     2281 tcggctaatc gatgaacaag ggaaactgac tcccgagtta gaagcagaaa tcacccaagc
     2341 cgtgaaaatg caacaagtgg aagatttgta tcgtccatac aaacaaaaac gacggacaaa
     2401 agcaacgatt gctaaagaaa aaggacttga accactcgcg aagtggctga tgcagcttac
     2461 agatggagag gtgcaatctg aagcagaaaa atatattgac aaagaaaaag aagtctcttc
     2521 tgccgaagaa gcgttgcacg gtgctcatga gattattgct gaacaagtca gcgataatgc
     2581 aaaatttcgg acttggattc ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt
     2641 caaggatgag caagcagatg aaaaaggcgt atatgaaatg tattatgact ttgcagaacc
     2701 cgtccataaa atggtttctc atcgaatatt agctactaat cgtggagaaa aagaagatat
     2761 tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat
     2821 agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta
     2881 taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc
     2941 agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc
     3001 actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc
     3061 agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc
     3121 agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca
     3181 agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc
     3241 tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc
     3301 ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga
     3361 aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat
     3421 cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc
     3481 tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac
     3541 agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat
     3601 tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc
     3661 acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa
     3721 aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt
     3781 aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac
     3841 acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga
     3901 tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt
     3961 attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac
     4021 ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact
     4081 tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt
     4141 aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt
     4201 gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag
     4261 aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc
     4321 aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc
     4381 atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca
     4441 tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag
     4501 atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga
     4561 cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc
     4621 gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat
     4681 ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa
     4741 ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt
     4801 tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt attttagctg
     4861 gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag
     4921 atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg
     4981 aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc
     5041 taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt
     5101 ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa
     5161 aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag
     5221 tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc
     5281 acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat
     5341 atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg
     5401 gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt
     5461 acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag
     5521 cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg
     5581 agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg
     5641 gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg
     5701 gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc
     5761 tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat
     5821 gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc
     5881 cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt
     5941 atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga
     6001 ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca
     6061 ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat
     6121 ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat
     6181 tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa
     6241 aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg
     6301 atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca
     6361 acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta
     6421 ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc
     6481 gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt
     6541 cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct
     6601 gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt
     6661 gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa
     6721 gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa
     6781 caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc
     6841 aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt
     6901 gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa
     6961 aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca
     7021 aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat
     7081 ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct
     7141 tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat
     7201 caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata
     7261 aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat
     7321 gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg
     7381 ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa
     7441 aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt
     7501 gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt
     7561 attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat
     7621 aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg
     7681 ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcgaa
     7741 agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta
     7801 tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt
     7861 ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt
     7921 tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa
     7981 gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt
     8041 agcggttact cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac
     8101 tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt
     8161 tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt
     8221 cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc
     8281 agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac
     8341 agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt
     8401 gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag
     8461 ttggggctat accgaattat ttggtatggt cccgcttttt cttttcgcat tccgcaggat
     8521 tctttgttag aatgaataag aactttacag taaggagtta agaagatgac aaaaaataaa
     8581 ttattactcg ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct tcacaattct
     8641 cttgaacgtt tcaaaaatag aaacggtctt catacaaatg caatctatgc attcaatacg
     8701 atgtttgaaa atgttatgca aaaagagcaa ccgactcatg ttctggttgc gttcgatgca
     8761 ggaaaaacga cttttcggac agaaatgtat gcggagtaca aaggtggaag atccaaaaca
     8821 cctggcgaat tcaaagaaca aatgccatat atacgtgatt tgttgacagg actaggggta
     8881 cagtattacg aattgcccaa ttatgaagca gatgatatta tcggtacttt ggcagaaaaa
     8941 gtagacaaag atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta
     9001 gctagtaaag aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat cgaaagctat
     9061 acaccagaac atgtagcgga aaaatatgat gggttgacgc caaaacagat tattgacatg
     9121 aagggactag ccggagatgc ctctgacaat atccccggtg tgactaaaat cggtgaaaaa
     9181 acagctatca agctattgaa acaatatggc tcagtcgaag gaatttatga acatatcgat
     9241 gaaatgaagc aaagcaaaat gaaagaaaat ctgataaatg acaaagagca ggctttctta
     9301 tcgaaaaaat tggcgaccat caatacttca gcgcctgtgg atgtctctat cgactcattg
     9361 aagtatgaag ggaaaaatct cgataaactc gttccgtttt ataaagaaat ggatttcaat
     9421 cagttccttt ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga tattttgttt
     9481 gaagtcgttc atgaattcaa agaggaaatg tttactacgg atatggcgtt gtacgtggaa
     9541 atgatgggcg acaattatca tacagaggaa atcgtcggag tagcgtgggg gacagaaaag
     9601 aaaatttatg tcacgaacga tctatctgta ttcgaaaacc gagcgttcca ttcttggatc
     9661 actgatccga ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt tgctttaaac
     9721 cgctatactg gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc ataccttctc
     9781 gatacgaatg aaaaaagcac agatatcgaa ggaatagctg ctcattacgg atacaacgat
     9841 atccaatcag atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc agaagaagag
     9901 gaactctttt ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa
     9961 ttgagccaag aactggcaga taaaaaccaa gaagatcttt tccggaaaat ggaactgcca
    10021 ttgtctatca ttttggcaga gatggaaata agcgggatca aagtagatgc gactcgctta
    10081 caagaaatga agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa
    10141 gaagccggcg aagaattcaa tttgaattca ccaaagcaat taggagtgat cttatttgaa
    10201 aaaatgggat tacccgttat caaaaaaaca aaaactggat attccacagc cgtagatgta
    10261 ctggaacagc ttcgtgaaca agcaccaatc gtagaggata tcctcaccta tcgtcaaatt
    10321 gcaaaaattc aatctaccta tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa
    10381 gtgcataccc gttatgtcca gacattgacc caaacaggac gattaagctc tgttgatcct
    10441 aatttgcaga acatcccgat tcgattagat gaagggagaa agatacgtca ggcatttgtt
    10501 cctagagaaa aagactggct gatttattct tctgactatt cacaaatcga gttacgcgta
    10561 cttgcgcata tctcaaatga tgaacatttg aaagctgcct tcctagaagg tcaagatatc
    10621 catgccagca cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt cacaccaaat
    10681 atgcgtaggc aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc tgattacgga
    10741 ctttcacaaa atctaggaat cagccgaaaa gcagcacagc aatacatcga tacgtacttt
    10801 gaaaaatacc caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa
    10861 ggctacgtcg aaacgctgta tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat
    10921 tacaatatcc gttcttttgc ggaacgaaca gctatcaata caccaatcca aggaagtgct
    10981 gctgatattt tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga aacaggtctt
    11041 caagctacga tgctgttaca agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa
    11101 cttgaatcat tagataaatt ggtgaaggaa gttatggaac aagctgtttc tcttcatgta
    11161 ccactgatca ctgacagcag ttggggaaaa acgtggtacg aagcaaaata aacattgttt
    11221 aagacagagc aatataatga aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa
    11281 ccgttcgtaa aggattggaa cggttagtag taggaaaaac gattcaaaaa gttcaggtct
    11341 tgtggcccaa gatcatcgag caaccagaga cacccatctt tgaggcttct cttgttggcg
    11401 aaaccatcca atccattgga cgtagaggga agtttttaat ttttcatctc gatcattgtg
    11461 aactgatctc acacttgcga atggaaggaa aatatcaatt tacgaaagaa aatacaccaa
    11521 tcgataaaca tacgcatgta cttttctttt ttgaggatgg aagccaactt cgctacaacg
    11581 atgtacgaaa atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca acttaccgag
    11641 gaatcatgaa attgggacct gagcctttgc cagatagctt cttattagca gatttcgcaa
    11701 atggactgaa aaagtcccac aaagcaatca agccattgct gctagaccaa cgtctagtga
    11761 ctggtctagg taatatttat gtagatgaag cattatggga agcgaagatc catccagaac
    11821 aaccggcgaa cacactgcgc cccaaagagg tagaacaatt acgtctatcg atcattgatg
    11881 ttttggatcg tgcaatagaa gccggaggta ccacgattcg cagttatttg aatgcattag
    11941 gagaatcagg cggatttcaa gtagcactgc atgtctatca gcaaactggc aaaccatgta
    12001 tacgttgtgg aacgccgatc gtgaaaacaa aagtggcaca aagggggacg cactattgtc
    12061 cgaattgtca aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga cgggtggaat
    12121 agctaccggt aaaagtacgg cagccaaagt ctttcagtct catggatttc ctttgatcga
    12181 tggcgatgtg attgccagag aaactgttga gcctggaacg cttgctctga ggaaaattgt
    12241 atcagtgttt ggccaagagg tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg
    12301 gatgattgtt tttccatcaa aggaaaaacg gcaaaaactt gatcagctat tagatccgtt
    12361 tatccgaaaa gcgataaaag atcaaatcgc ctctctaagt tccaatcatc ctttagtaat
    12421 tgtagatatc ccattgctct acgaaggtca ttatgaccac tatatggatg cagtagctgt
    12481 cgtttatact acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc aactgacgct
    12541 agaacaagcg cagcagagaa ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc
    12601 ggatattttg ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg
    12661 gctttttgct aatcgctttc tttgagtatc tgtgtcaatt ttgctatcct gagtatatag
    12721 aaagtgaggg atagcgatgg gatcattaaa aaatcaaaag gataaagtcg taggtaacgc
    12781 aaaagaaaaa atcggcgaga tgctcaataa agaaaatcta atcgatgaag gtcgtacgca
    12841 atcagaaaca gcagaagaaa aagaaaagca gtaccaagca agaatcgatg aaatagaaga
    12901 ccagcaggca gaagaagaag cgaagcggca agatttgact caagaacgaa aaaatccgac
    12961 acctgatcgt agtaaaacac cgctcccaga aagtgaccac atttccgatg agcaaaaaat
    13021 cgaggaacag cgaatgaaac actatacagg aatcgaagag aagttataaa atagtaaaaa
    13081 atcgagatgt gacttgaagc agatgagaag gtgttcatct gcttcaagtt ttttgtgcta
    13141 gatgactaaa tcagcttttg tgtaagttat ttctcagtaa tcaaggtgat cagtattgga
    13201 aaattgaaat ttagtaagga caggggacac aattacgcat gaaagtagaa aaaattgttc
    13261 ttatatccgt gttttttggg taggacagtc gcgtttgtga tttgtttcgt gttataatac
    13321 aactagttgt aaagaaagac acatccttta tataaaatgc gatgcaagag gtgagacaaa
    13381 atgagatgtc ccagatgtca acataataat tcacgagtaa tcgacagccg tcaagcagat
    13441 gatggacgtg cgatccgtcg ccgtcgcgaa tgtgagaatt gttcgttccg attcactaca
    13501 tttgaacgaa ttgaagctgc tccgttatta gtaatcaaga aaaacgggga acgcgaagag
    13561 ttcaatcgcg ataaaatttt aagaggactc atccgttcag cagaaaaaag acctgtagct
    13621 atggaacaaa tggaacaaat cgtcaaccat gtcgaaaacc gcgtacgcag tctcggagaa
    13681 aacgaagtat cgacgaatct aattggtgaa tatgtgatgg aagatttagt tgatttagac
    13741 gaaatcgctt atattcgttt tgcaagtgtt tatcgccagt tcaaagatat gtctgttttc
    13801 ttaaaagagt tgcaagatat cgtggataaa gcaaaagaaa ataataaaga gaataataaa
    13861 gaaaacaatt aagaaacaaa aaggaggcat ttccttggaa aatgcttgga aagagcttca
    13921 accaaaaaat atttaccaag gaagaaaagc ggctccgctg agagaagaca gcaagaacgc
    13981 cttgctgtat ctttatcagc cactaatcgg cggagaagcc ttgagtatct atttcaccct
    14041 gttatcagaa atctctcaag aatctggcaa aggtcctgaa gggatgcatg cagatctgct
    14101 tagtagtcta ggctgcggta tcccgcaatt ttatgatgca agaaaaaagc tagaaggaat
    14161 cggcttactg gatgtttatt ttaaagaaga tagtgatctg ggacctcgtt ttatttatga
    14221 gctgttagaa ccactcattc ctaaagcatt tttcaaagac tctgttctgg cttttcttct
    14281 tctggaaaaa gttggtgaac gacgattcca gaagcttacc caattctttg aacctaaaag
    14341 gcatacagca gagggatatc aaaaaatcac aaaaaaattt ttagatgttt atcgcttcaa
    14401 tgaagcagtg tacgctgcta atcaagaaaa catcgaatcg ctgaataaat cttttgaaga
    14461 atctgaatca gtagcattac ttgaagcaga ttcgacattc gattggcagt ttttgaatga
    14521 ttggctggca aaaaaacata ttgcaagtct tgatgatcca atcatcaaac aagtcgacat
    14581 gtatcatcag ctttacggtt tcgatgaatt gacacttggc gaactgatca gtcaatctta
    14641 tgactttagt gaacaaaaag tatctgaaaa agaactgcaa aaagccattt tggcaatgca
    14701 gagacgtccg gtgaagcaaa caaaagaaac agttgcggtt tctgaaaaca gtaatgaacc
    14761 ctcgtcaaac gaacatttga caaacgcagc gcttcagctg ataaaagaag cacagagcat
    14821 tccaccgatg aaatatctgg aagcaatcaa gtcagaaaaa ggcggatatg tttctaaaca
    14881 agaaaactgg ttactgcaag atcttgtttc tcaatcaggt ctttccagta gcgtgatcaa
    14941 tgttttgctg aattatgtac tagtgatcaa gaataatgct agcttaaatg ccagttatgt
    15001 aaatacagtg gcaaatgaat gggcacaaaa gaaaatcgcc acagcagaag aagcgatcca
    15061 gcacattcgg caaataagta atcaagcaaa acaatcaaaa caaaagaagc aaacaaatta
    15121 tcaaactggc aagcgaaatg ttcgacggga aaagcttcct gattgggtca accagccaaa
    15181 agatgaaaaa cagatcagca gcgagaaaaa agcagagatt gaccgccgtt ttaaagaata
    15241 cctggcaaaa aaggagggtg atatctagtg gaagacgtag gcaaagaatt gaagaggatc
    15301 atcagcaaac gcaattatca agaaaagtat gctgaaatga tcgctgaagt cttaaatgat
    15361 gcggatgttc aagcttttct aagcgcacac caagaagaat tgacacaagc ggatattgaa
    15421 aaaagttacg ctaaattgta tgaattcgtg caagaaaagc gtaaatatca aaaaaacgat
    15481 ccaacgatga tcgctcccgg ctatgagccg aagctgacgt tgaattttca ttatgtggat
    15541 gtcacttatg taccaacgga agcattgtta gcacggcaaa gacaagaaga aatcagaagc
    15601 cgagtaaaag caatggatat gccaaaagat attcaatcgg cttcttttga aaactttgaa
    15661 cgtacagatg gacgaggata tgcgagctta gaagccctag attttgtaga gaaatatact
    15721 gctgatccaa aagggtatca taaaggactg tatctagtag gaagttttgg tattggaaaa
    15781 acatatttat taggagcaac agcgagagaa ttagcgattg ctggattcac cacgacgcta
    15841 gtccattttc cgacatttgc cgttgagatg aaacaggcaa tcggaaaaga tcaagtggca
    15901 gaaaagttag atgcagtgaa acgttcacct attttgatga tcgatgacat tggtgcagat
    15961 gcgatgagta gttggatacg agacgaagta ttcggtgtta tccttcaata tcggatgcag
    16021 gaacagttac caaccttctt ctcatcgaat tttacaatgg atgaattgga acagcatttg
    16081 tccgtcactc aacgtggaga tgaagagccg ctaaaagcga aacggataat ggaacgcata
    16141 cgttatttga caaaagaaat cgaaatgact ggtcgcaacc gacgcaatgg ttgaacagtt
    16201 gacaagtcca actgacattt gacctagtag taaaagcagg tcagcgaatc attatatgga
    16261 cagaaatgac cggagacaat caccgcaaca agtaatgcag aaacttaaac ggaggatttt
    16321 ttatgagggc tattttttct agtatatctg tagttggtct attttttctt ttagcaggct
    16381 gcggtagcgc agatagcaac acaggttctg ttgattcgat tgatgcgggt caaacagtaa
    16441 aaacaagcag tacgaaaacg gagagacaaa gcatatttgt cggaacgatt gaaagtatta
    16501 ctcaagtaga agcagaagaa gaaacgcttc agttgaaact cgtcgatgtc atggccaaag
    16561 aagatccagc gaatatggga acgtcattca gcaatgacgg agtgatctta aatgctactg
    16621 ctgaaaaaat aagcgtggac ataaatgaat tgaaaatcgg tgacaaagtc caatttacat
    16681 tagttgagat gccgatcatg acgatgtcta tcccaccgca agttcctgga gattcgatta
    16741 ttgatgtttc attgaacaaa taaccaataa cattttttca aaaataagaa aatgttgttt
    16801 tatagaacga aaaggtatat tttttaagga gatatgggaa tttt
//
LOCUS       DAFEHS010000060        16654 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFEHS010000060
VERSION     DAFEHS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.062"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(312..611)
                     /locus_tag="LP327_002010"
     CDS             complement(312..611)
                     /locus_tag="LP327_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726117.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWGSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(617..847)
                     /locus_tag="LP327_002011"
     CDS             complement(617..847)
                     /locus_tag="LP327_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726118.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1097..1297)
                     /locus_tag="LP327_002012"
     CDS             complement(1097..1297)
                     /locus_tag="LP327_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL1726119.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(1602..2834)
                     /locus_tag="LP327_002013"
     CDS             complement(1602..2834)
                     /locus_tag="LP327_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBL1726120.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3089..3658
                     /locus_tag="LP327_002014"
     CDS             3089..3658
                     /locus_tag="LP327_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL1726121.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3836..4276)
                     /locus_tag="LP327_002015"
     CDS             complement(3836..4276)
                     /locus_tag="LP327_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBL1726122.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            4434..5198
                     /gene="map"
                     /locus_tag="LP327_002016"
     CDS             4434..5198
                     /gene="map"
                     /locus_tag="LP327_002016"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBL1726123.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5230..6153
                     /locus_tag="LP327_002017"
     CDS             5230..6153
                     /locus_tag="LP327_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBL1726124.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6229..7368
                     /locus_tag="LP327_002018"
     CDS             6229..7368
                     /locus_tag="LP327_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBL1726125.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            7361..8161
                     /locus_tag="LP327_002019"
     CDS             7361..8161
                     /locus_tag="LP327_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL1726126.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8161..8988
                     /locus_tag="LP327_002020"
     CDS             8161..8988
                     /locus_tag="LP327_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL1726127.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8966..9700
                     /locus_tag="LP327_002021"
     CDS             8966..9700
                     /locus_tag="LP327_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL1726128.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9800..10666
                     /gene="rfbA"
                     /locus_tag="LP327_002022"
     CDS             9800..10666
                     /gene="rfbA"
                     /locus_tag="LP327_002022"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBL1726129.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10680..11252
                     /gene="rfbC"
                     /locus_tag="LP327_002023"
     CDS             10680..11252
                     /gene="rfbC"
                     /locus_tag="LP327_002023"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBL1726130.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11274..12302
                     /gene="rfbB"
                     /locus_tag="LP327_002024"
     CDS             11274..12302
                     /gene="rfbB"
                     /locus_tag="LP327_002024"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBL1726131.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            12400..13251
                     /gene="rfbD"
                     /locus_tag="LP327_002025"
     CDS             12400..13251
                     /gene="rfbD"
                     /locus_tag="LP327_002025"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBL1726132.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13285..15318
                     /locus_tag="LP327_002026"
     CDS             13285..15318
                     /locus_tag="LP327_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726133.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            15362..16642
                     /locus_tag="LP327_002027"
     CDS             15362..16642
                     /locus_tag="LP327_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBL1726134.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         5149 a         2865 c         3388 g         5252 t
ORIGIN      
        1 attgtgagat aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag
       61 tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga
      121 aaaaaagtga gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga
      181 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
      241 cttaaaaaat atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta
      301 ggtagcctac tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca
      361 taacgccgat gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat
      421 acgtctttct tcattctagt tttcgcagca gggattttaa agtagttctt attagaatag
      481 tagagattaa ttaataagct atcttcgatt gctaaaaaga aaacttctga accccacacc
      541 ttataaaaat ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca
      601 ttaaaaagca taaccattac tccgatctat ttttaaactt agtctctacc tctaatatat
      661 atcgagtttt tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttgtt
      721 tattttcgga aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt
      781 cgttgctttg ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt
      841 ccatcataaa gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga
      901 atttattata ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa
      961 atcgaattta ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata
     1021 acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc
     1081 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
     1141 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
     1201 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
     1261 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
     1321 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
     1381 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
     1441 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
     1501 aaaacggtgg ggttgtttag cgaaataaag gatctaagcc aatagcttag atcctttatt
     1561 ctatgcttta tattattttt tgcatgtgtg cgaaagtata ctcaagccca atcgccattt
     1621 cggaaaatcg gtactgtaga gccatcttca cggattccgt cgatatccat cttgtcagat
     1681 cctaccatga aatctacgtg ggtttgactt ctattcaatc cagcttctgc taattcttca
     1741 tcggacattt ctgtaccgcc tttcacactg aaggcataag cagaacctag tgctaagtga
     1801 ttcgatgcgt tttcatcaaa taatgtattg aaaaagatga tgccagattg agaaataggt
     1861 gaagggtcag gtacaagtgc gacttctcct aaacgacgcg caccttcatc tgtatcaagc
     1921 agttttgcta agacatcttc tccttgttcg gcagaaaaat caacaacttt tccatcttta
     1981 aaagtaaatt tcatacccga gatgatggtg ccagcataac tcagtggctt tgtacttgaa
     2041 atgtaaccat ctactcgatg actgtcaggt gctgtgaaca cttcttccgt cggcatattt
     2101 gccatgaatt tttctccacg agcattatag cttccagcgc cttcccaaag atggttttta
     2161 ggcaatccga taatgatatc tgtaccggga gcagtatagt gaagggcaga aaattgttct
     2221 cgattcaatt cttctgcttt ttttgctaat ttttcatcat gcttcttcca tgctaagaca
     2281 gggtcttctt catagacacg agtcgttttg aaaatctgat cccataatgc ctcaacttgt
     2341 ttttcttctg gcagttctgg gaagactttt gctgcccatt gtttaccagc cgcagccacg
     2401 acagtccaac tgactttatt tgcttgagtt gccttgcgta aattcattag tgctttgcca
     2461 ttcgctgatt gataagacgc tactcgatgg ctatctacac cagcaaatgc atcgggatcg
     2521 gcagaaacaa cgctgatcct gcttgctcct ttttcaagcc attcatccgc ttgatcgatt
     2581 ttagattgtg gtacattctc aatacgatcc gttgcagcat gtaaaagaaa ttctctttgg
     2641 atctggtcgt cagtccattg aacgataacc tcagcggctc ctagtttata agcttcttgt
     2701 gtgatcaatc gagcaagggg tgcctgttcc acgctgatct gtaaaacaac tgtgtgtcct
     2761 ttttctgtgg ctacgccagt ttctgcaatt aagcgtgcat atttttttag aagttcatta
     2821 aaatcagata acataataaa aattcctcct caccattttt tctggtaact attagcatga
     2881 taccatttaa ttatgattca aacaatgagg ctttaaataa aaaaaggagg ttattttttt
     2941 aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa gaaaatcaaa
     3001 aactaatttt gcatatcaaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt
     3061 tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca
     3121 gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag
     3181 aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat
     3241 ggatgacttg agacaagctc tttttgagca gatctatgat catttacaat atgaagtgtt
     3301 cactgttgag catacaggga atacgattat cgatcttgct tttaattata tccattttgc
     3361 aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga
     3421 gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt
     3481 gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg
     3541 gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat
     3601 gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat
     3661 atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt
     3721 cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga
     3781 ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa
     3841 tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat
     3901 tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat
     3961 cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac
     4021 cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat
     4081 caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga
     4141 attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa
     4201 atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga
     4261 tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata
     4321 ccaaaacatc attgaaaaga aaataaaacc tagagacacc tggggaatgt catttggcta
     4381 aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta
     4441 ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg
     4501 tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat
     4561 ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt
     4621 atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa
     4681 agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca
     4741 tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga
     4801 tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga
     4861 ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc
     4921 gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt
     4981 acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta
     5041 tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa
     5101 gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa
     5161 gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg
     5221 gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt
     5281 gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct
     5341 tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt
     5401 ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt
     5461 taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta
     5521 tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt
     5581 gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc
     5641 tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg
     5701 ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc
     5761 gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc
     5821 ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg
     5881 atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat
     5941 tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg
     6001 cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa
     6061 tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa
     6121 ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat
     6181 atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
     6241 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
     6301 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
     6361 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
     6421 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
     6481 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
     6541 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
     6601 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
     6661 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
     6721 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
     6781 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
     6841 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
     6901 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
     6961 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
     7021 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
     7081 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
     7141 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg
     7201 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
     7261 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
     7321 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
     7381 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
     7441 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
     7501 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
     7561 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
     7621 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
     7681 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
     7741 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
     7801 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
     7861 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
     7921 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
     7981 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
     8041 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
     8101 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
     8161 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
     8221 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
     8281 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
     8341 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
     8401 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
     8461 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
     8521 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
     8581 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
     8641 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
     8701 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
     8761 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
     8821 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
     8881 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
     8941 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
     9001 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
     9061 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
     9121 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
     9181 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
     9241 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
     9301 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
     9361 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
     9421 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
     9481 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
     9541 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
     9601 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
     9661 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
     9721 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
     9781 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
     9841 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
     9901 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
     9961 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
    10021 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
    10081 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
    10141 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
    10201 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
    10261 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
    10321 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
    10381 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
    10441 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
    10501 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
    10561 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
    10621 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
    10681 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
    10741 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
    10801 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
    10861 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
    10921 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
    10981 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
    11041 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
    11101 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
    11161 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
    11221 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
    11281 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
    11341 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
    11401 agaacttggc tggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
    11461 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
    11521 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
    11581 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
    11641 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
    11701 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
    11761 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
    11821 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
    11881 taacgaatat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
    11941 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
    12001 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
    12061 tgatcttaga aatgatgggt caaccaaaag atgcgtacga ccatgtaaac gaccgtgcag
    12121 gacacgactt gcgttatgca atcgattcga caaaattacg ggaagagtta ggatggaagc
    12181 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
    12241 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
    12301 aatacagaga aaatgtcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
    12361 tctctttttt aaaataaaaa tcaaggagcg gataatcata tgtttttatt aactggagga
    12421 aatgggcaac taggtacaga attgcgccat ttattagatg aaaaaaatct gcaatatgtt
    12481 tcaacagatg caaaagactt ggatatcaca gatgccgaga aaacaatggc ttacatcaca
    12541 gaattaaaac ctgatgttat tttccattgt gcagcttaca cagcagtaga taaagcagaa
    12601 gaagaagcaa aagaattaga tgagaagatc aatgttgatg gtacaagaaa tgtagcactt
    12661 gcagcaaaag ctgtgaatgc tcaattcgtc tatatcagta cagactatgt tttcgatgga
    12721 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
    12781 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
    12841 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
    12901 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
    12961 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
    13021 catttgtcga acgaaaatag ctgttcttgg tatgaattcg caaaagaaat attgaaagat
    13081 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
    13141 cagtattcag tcatgagcct gaaaaagaca gaagagttag gatttgtcat accaacttgg
    13201 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
    13261 atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa
    13321 aagccaaaag cgaaccaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc
    13381 tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca
    13441 cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt
    13501 tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc
    13561 tcacaagctg aagaggactt tccgttggtg aacgaggatt attcattaaa cggacaaaac
    13621 atgatcattg cgtataattc tcctgcaaag gatattacgg ttatcggaaa accattcaac
    13681 tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta
    13741 gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag aaaaaaagct
    13801 ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg
    13861 caacatgtgg gcgatttgat tttctttact ttgggactga tgactgcttt ttatcattat
    13921 ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc
    13981 ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg
    14041 attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg
    14101 gatgtagtgt tgattggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg
    14161 ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta
    14221 agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt
    14281 ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt
    14341 ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag
    14401 ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg
    14461 ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc
    14521 cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga
    14581 gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat
    14641 ttctatgcga ttacgcattc gaaaatagat aattattttt cggattttga aaccatcgca
    14701 gttgctgtgt tgggaacagt gattttagca tttgcattat ttggctggag acgcttgttt
    14761 tacctcgctt ttgccgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa
    14821 ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa
    14881 gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga
    14941 gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa ggaaatcgat
    15001 ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct
    15061 aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt
    15121 gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac
    15181 aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg
    15241 atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac tcaggaaaat
    15301 cagctagtcg tacagtaata ggctagctga gaataatcct gaaatattag tagaaaggag
    15361 agtggagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt
    15421 gcttttgttg ctgacgctag tcttttttgt ttggacagca gggttaggtt ttccagcaac
    15481 tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat
    15541 tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct
    15601 tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg gagatttact
    15661 gtttcctttg attattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga
    15721 tcgcggtatt tatctgattg tctttacggt cttcctcgga atcacgttat atcgtgtatt
    15781 tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga
    15841 tatttggatc aatacgaata caatcggaag ttctttgatg actcttgctt tattgatcag
    15901 tggatttgcc agttcttttg aacgatggta tgtacgaatc atcggtgcag tagcagttgt
    15961 tgcagctttt ctcgggattt gggtctgcca atctagaggt gcattagtcg cgttgattgt
    16021 ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat
    16081 tgcttacacg gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga
    16141 aaaagtcaat ttgtttaccg gtcgagaaga tatttggcat aaattctatc agacgctagg
    16201 agaaaaatct gaacaagttt tactaggaat gaaaacattt atcttccaac gaggaaacca
    16261 atttttagga aaccacaata gttataattc tattttgaat atctatggat tgataggatt
    16321 tgggattgct actttgctgc ttatcctttt tattggtcgc ttaacgttga aagcagattt
    16381 atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc agtcatttat
    16441 ggaggataca ttgacttcat acccatgggt tccgattatt tatatcctgt tggctatggc
    16501 cgttcatcga tatgatgaac cagaagttgc ggaggaattc tatgttatgg aagaggcata
    16561 tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg ctcattcttc
    16621 caggatccaa cgctatcatt gataggcaca aaat
//
LOCUS       DAFEHS010000061        16382 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFEHS010000061
VERSION     DAFEHS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.063"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            13..1014
                     /locus_tag="LP327_002028"
     CDS             13..1014
                     /locus_tag="LP327_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HBL1726135.1"
                     /translation="MNTTDNRRLYERFLAVKLVLEGHTRKEAGHTIGRDEHTVSHYVK
                     NYCHNGIQGLVSKKQSGRPSRLTDEQEAELGQIVAYHTPEEVGFKSRANWTLAIVCDL
                     ILREWSYGYTQKGVADLLHRLGLSWTRPTYTFKKADSKKQQVFLEETFPSLKKKLLNS
                     DIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGTLDYEAGRIICTE
                     EGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEENARRLALIFLPP
                     YSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERPLEVIDRLCVRM"
     gene            1034..1369
                     /locus_tag="LP327_002029"
                     /pseudo
     CDS             1034..1369
                     /locus_tag="LP327_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1415..1594)
                     /locus_tag="LP327_002030"
     CDS             complement(1415..1594)
                     /locus_tag="LP327_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726136.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(2081..2281)
                     /locus_tag="LP327_002031"
                     /pseudo
     CDS             complement(2081..2281)
                     /locus_tag="LP327_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2488..2697)
                     /locus_tag="LP327_002032"
     CDS             complement(2488..2697)
                     /locus_tag="LP327_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726137.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            complement(2871..3488)
                     /locus_tag="LP327_002033"
     CDS             complement(2871..3488)
                     /locus_tag="LP327_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL1726138.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTPKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            complement(3795..4454)
                     /locus_tag="LP327_002034"
     CDS             complement(3795..4454)
                     /locus_tag="LP327_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HBL1726139.1"
                     /translation="MADKRKEKIQKLLALAADANDEESMSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLASIIAQNFRTEFYYREGSPIDLCFTGLA
                     SDVQIAEITFQYASGSVSYCARKYMQQPEIKRKRKRKWQLKQDYIQGYLSGLSKVFKQ
                     QVLTNGYELALQLPEAVKSYMEDLGLVPGKDTSHKVKDNEAYFHGYQEGLKFKKTELL
                     E"
     gene            complement(4464..4730)
                     /locus_tag="LP327_002035"
     CDS             complement(4464..4730)
                     /locus_tag="LP327_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726140.1"
                     /translation="MGTSFIDRVIVQVKEERKITMKYNSLDHKRMWVEKLEEDLEQLE
                     LLGYNKTSTVYKEGLKRTKKVQKELATLESSVQKKEVELTHAYL"
     gene            complement(4712..5101)
                     /locus_tag="LP327_002036"
     CDS             complement(4712..5101)
                     /locus_tag="LP327_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726141.1"
                     /translation="MKKKQTKLSLADILTQLTATEDGVVEFERSEIAVVDDRYFKMPY
                     FFDQAKVICLCGYDGVRDYFGITEEKVVWVNNHTGLGALAFEGTVLDNISIVFEEESF
                     TLECDNLIRYIDPEFYEDKNLAWELAL"
     gene            complement(5169..5348)
                     /locus_tag="LP327_002037"
     CDS             complement(5169..5348)
                     /locus_tag="LP327_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726142.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(5791..6228)
                     /locus_tag="LP327_002038"
     CDS             complement(5791..6228)
                     /locus_tag="LP327_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726143.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(6244..6495)
                     /locus_tag="LP327_002039"
     CDS             complement(6244..6495)
                     /locus_tag="LP327_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726144.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            complement(6495..6701)
                     /locus_tag="LP327_002040"
     CDS             complement(6495..6701)
                     /locus_tag="LP327_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL1726145.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            complement(7150..7410)
                     /locus_tag="LP327_002041"
     CDS             complement(7150..7410)
                     /locus_tag="LP327_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726146.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            7553..7687
                     /locus_tag="LP327_002042"
     CDS             7553..7687
                     /locus_tag="LP327_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL1726147.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            complement(7846..8103)
                     /locus_tag="LP327_002043"
     CDS             complement(7846..8103)
                     /locus_tag="LP327_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726148.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8125..8609)
                     /gene="ssb"
                     /locus_tag="LP327_002044"
                     /pseudo
     CDS             complement(8125..8609)
                     /gene="ssb"
                     /locus_tag="LP327_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(8612..9511)
                     /locus_tag="LP327_002045"
     CDS             complement(8612..9511)
                     /locus_tag="LP327_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL1726149.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            complement(9524..10120)
                     /locus_tag="LP327_002046"
     CDS             complement(9524..10120)
                     /locus_tag="LP327_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL1726150.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            complement(10201..10350)
                     /locus_tag="LP327_002047"
     CDS             complement(10201..10350)
                     /locus_tag="LP327_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726151.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(10466..12475)
                     /gene="topB"
                     /locus_tag="LP327_002048"
                     /pseudo
     CDS             complement(10466..12475)
                     /gene="topB"
                     /locus_tag="LP327_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            complement(12486..13946)
                     /locus_tag="LP327_002049"
     CDS             complement(12486..13946)
                     /locus_tag="LP327_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL1726152.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVQDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGKQQERE"
     gene            complement(14007..16379)
                     /locus_tag="LP327_002050"
     CDS             complement(14007..16379)
                     /locus_tag="LP327_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726153.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNDQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQD
                     EKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         4296 a         3440 c         2799 g         5847 t
ORIGIN      
        1 ttggaaaaag cgatgaatac gacagacaac cgccggttgt acgagcgttt cctggcagtc
       61 aagttggttc ttgaaggcca cacccggaaa gaagccgggc acaccatcgg ccgcgacgag
      121 catactgtca gccactatgt gaaaaactac tgccataatg gaatccaagg tttagtctcc
      181 aaaaaacaaa gcggacgacc ttcacggctg acagatgaac aggaagcgga actgggccaa
      241 atcgtcgcct accacactcc ggaggaagta ggtttcaaat cccgtgccaa ttggacattg
      301 gcgattgtgt gtgacctgat tctccgggaa tggagttatg gatatacgca aaaaggcgtg
      361 gcggacctcc ttcaccggct aggactaagc tggacccgtc cgacgtatac cttcaaaaaa
      421 gcggattcca agaaacaaca ggtatttttg gaagaaacct tcccgagcct aaaaaaaaaa
      481 ttgctgaata gcgatattga ccacctccta ttcgaggacg aaaccatgat ccgggattac
      541 caagccattg gctgcacctg gtttctccgc ggccaccagc gaaaaatccc cacttatgga
      601 caacacaaag gggtaaaatt gataggcacc ttggattatg aagccggtcg gatcatttgt
      661 acagaagaag gggcatacga cgcagcggcc ttcctccgtt ttctggagcg gacgttgcgg
      721 gaatacccca ccggaaaaat cgtgatggta ttggataatg cccttatcca ccacgccaag
      781 ctgatccagt cttttcttga agaaaacgct aggcgcctgg cactcatctt cctccctccc
      841 tacagccctc agttgaacct aatggaaggc gtttggaagt ggctcaagga atcggtcatc
      901 aacaatgttt tttttgacca cgtccagaaa atcaaacagt cggtccgcgg tttcttagcc
      961 gatgtaaacg agcgtcccct tgaggtgatt gaccgactgt gcgtgcggat gtaaagtatt
     1021 taattcaacc tatatagatg cacagttttt agattttaat actggaaatg aactcctaga
     1081 tactgctatg ttcgatatgt ttttatcatt gctaagttat attgcacaaa acgaacgtga
     1141 gaaaatccgt gaacgtcagc ggcaaggagt attattggcc aaaaaagctg gtcgatataa
     1201 ggggcgacca attgaatatt ctgagaactc aacagatccg caaaaacgat tagtctttaa
     1261 aactgtggtg gacatgttaa atcaaggagt tccagttgca gaaattgcca aagaaaatgg
     1321 tctctctcga ccaaccatat ataaaataaa gaaagcgaat gatttgtaga gtttatacta
     1381 cattcattcg cttttctacc gtgcattttc gtttctatgt tccatgcacc ccatattctc
     1441 tatgaaggag tttcaatgtc atattctttt tcattagttc tttatgaaca tattcccagt
     1501 ctacaggaag gtaacctcgt gctgttgttt gtttttccgg aaaaagatat tcctgcagcc
     1561 aagtatcgct catatcagcg gttagttcag tcaccccctt cctgtctgct cgagaagtaa
     1621 cttctcgaat cgtatgtctg gagcttccca cagttgaact aatcgttcgt tgagaaatac
     1681 cggaaaaata aagctccaaa atttttcgat agtgaaccaa taaaataaca tcctgtataa
     1741 gttgtcatac cgaaaggtat gcctttctat tatacagaag gttattcatc tattaaatat
     1801 gaaagtggag cagtttgttg ccctggatgg agctatctat tgccttgagt ggagcagtta
     1861 ttcacctcac tggagctatt tatctgcaat actcaacttg tttaaatttt ttttcagtaa
     1921 ctaataggag tcaatggtaa tacgtacatc catgtattct aaagtggtac aaacaagggg
     1981 attatccatc ggttttcttt caaacgagct tagaattgcc ctgagccaac taataaatag
     2041 caaaacaagc gaaccgagag aaggcttcgc ttgtttctga tcaattctgt ttaattttat
     2101 ctaggatcac ttcaaatgtt tcaaaggtat ctatatctaa ctggcacagc caaagtaagt
     2161 atttcttttc cttatatgtc tagtcaacag tggggtagga ggtgatcaaa ttattgtatc
     2221 ttgctctttg ttcacttgat gctagctgcc agcgtttctc aaatattttt tcttcttcca
     2281 ttatcgatcc cccaaaaaga gcgctgcctg aaacttcaat aacgggcagc gcaagtctaa
     2341 ttatgcagat ctatgtatag cataacaaac aaatatgaaa aaacttagaa ggtagtagaa
     2401 gttctgccgt cttttgcttt gctttcgttc ttaatgtgtt tcattctagg atatctcaaa
     2461 ataaaaacta tgcgtggtgt tctcatatta attacttgtt tttttctctt ttaagtacat
     2521 taatacttca tattcctcaa catgcttact tacaaaagca ttataaaatg aagtacctaa
     2581 aaccatagca atcaatgtta atatagaata gctgacaata ccccagtctc cagaaaacat
     2641 ttcaggaaac actgcttgtg gaagacctag tattagaaat gaaatataac agatcataac
     2701 tacatttaat aagtttttat tcctatccaa tgcaactttt ttttcggcta taattaaatt
     2761 caattcacta gtttccatat tcataatttc attatagtaa ttctgcatat tttgatttga
     2821 aaatctaatt tctttcataa aaatcccttt cttgcaaaat tatattagcc tcaactgaaa
     2881 gatttatttt tttgctccct aacttcatta gtaataccat gtttcattcg atatcttcta
     2941 aacgttctac gattaatccc tgttagctct tcaacttctt tatctgtaga tccagtcaaa
     3001 aacaaattaa atgctaattg aagtcgcgga tcattctcac gatattttgg cgtacctccc
     3061 ttgtatttcc cttttgcttt agcgagtgca attccttgtt gttgcgtttc gcgaatatac
     3121 cgacgttctt cttcggcctg gtacttgtat agttcgataa ttaagttatt aagtaatcga
     3181 cgtaaattat catcagtgat acctgttaac gctggcaaat tcaaaacttc cagtgtagct
     3241 ttcttcagct gaattaaatt catcgtttct gttagctctt tattatttcg tccaagtcgt
     3301 tttaattctg tcacaacgac aatatcacct tcacgtatga atttcatcat ttcttgaaat
     3361 ccttctcgat tggtatcttt cccacttgct ttatcactaa aaattttatc acactctgat
     3421 aataattcta attgtcggtc taaattttga tctaagctgc ttactcgagc atacccaatt
     3481 ttcgccataa taaaactcct ttaaaattct gaaatccgga tcatttttaa attctgttgc
     3541 ccaactctgt tgtcattttt ctagataaat ggcataaatt ttcagcgcta agtgacaaaa
     3601 gcaactaatt tttcatgata gatttttaaa tatctcaatc tatctccaaa attagttgct
     3661 tttgtagcta tagttgacta tgaatatgac aaataattag aaaaaactgt gccaattaaa
     3721 tataaaaatg acactcattt acattcaata gaacaccaag agcccaacag ggtataccct
     3781 gttgggcgca cttcttattc tagtaattct gtttttttaa attttaatcc ttcctggtat
     3841 ccatggaagt aagcttcatt atcttttact ttgtgtgaag tgtcttttcc aggaactaaa
     3901 ccgagatctt ccatgtaaga cttcacggct tcaggaagct gtaatgctaa ttcatatccg
     3961 tttgtaagta cctgctgttt aaatacttta gataaaccac tgagatagcc ttgaatatag
     4021 tcttgcttta attgccattt tcgttttctc ttgcgtttga tctcaggttg ttgcatatac
     4081 tttcttgcac aataggagac acttccgctt gcgtattgaa aagtaatttc agcgatttgt
     4141 acgtctgaag ctaggccagt aaaacataaa tcgattggtg atccttctcg ataataaaat
     4201 tctgttctaa aattttgcgc aataatcgac gctaaacgat acagccattt ttgcggacgt
     4261 ccacgataga tcacgtgcgt ttctactgct tctatccgtt gctgctcatg ataattgaaa
     4321 atctcatctt cagtaagatc atagtccatc atcaattgct gggctttggc taatgcactc
     4381 attgattcct catcatttgc atccgccgca agcgccagta atttttgaat cttctctttt
     4441 ctcttgtctg ccattttcta ctcctaaagg taagcgtgtg ttaattctac ttcttttttc
     4501 tgaacgctac tttccagtgt tgctagttct ttttgtactt ttttcgttcg ttttagccct
     4561 tctttataca ccgttgaagt cttattataa cctaacaatt ccaactgttc taagtcttcc
     4621 tctaatttct ctacccacat tcttttatgg tctaatgaat tgtatttcat ggtgatcttc
     4681 ctttcttcct ttacttgcac tatcactcta tctataaagc tagttcccat gccaggtttt
     4741 tatcctcata gaattctgga tcgatatatc ggattaaatt atcgcattca agcgtaaagc
     4801 tttcttcctc gaatacaata gagatattgt ccaatacagt tccttcaaag gctaacgcac
     4861 ctaatcctgt atgattgttt acccaaacga ctttttcttc agtaattcca aagtaatcac
     4921 gtaccccatc gtagccacaa aggcaaatga cctttgcttg gtcaaagaag taaggcattt
     4981 taaagtaacg atcatctact accgcaattt cagatcgttc aaattctaca acaccatctt
     5041 cagttgcggt taactgtgtt aaaatatctg ctaacgatag tttagtttgt tttttcttca
     5101 tcttatttcc accttttcca ttgtttaaaa gtgatatcac ttttaggttt tagtataaaa
     5161 aagtgacgtc actttttgag tgctggtacc tcaaatcctg ctttttcttg ggcaatcaat
     5221 aaatctaacg caatacacat aatcctggat tcacttttgc cgtacttctg ctttagttgt
     5281 tgcaattctt tttgtctctt ttcatccaac gtaacaggta accgtttatt tcctattgca
     5341 atcggcattg gtactctctc ctttcatcga aataacccta tgaaaaatat ggctgcaatc
     5401 aacctgaata ttagactcac aagggttaac acaaagcgcc aaaagatcaa acaaaatacc
     5461 agtattccta agaatactac gaagtttaaa tgtagagctg caccatgacg cataagtatc
     5521 tcgccaacaa acagtacgaa caatagagga atgattttat tcatatggat ctccttcttt
     5581 ctcttaggtt atcgggcttt gcccgcttat ttcgcaccac ctaagcggcg ggggcggttg
     5641 gagcaattgg tgcgaaataa gaaccattcc cttaatttca aggttttttg ttcttatttc
     5701 acacatcctc acttttcggt ataaatggtt attgaccttt tttcctttca aatcgttgat
     5761 atggaatccg ttgttagttg ataaatcacc ttattcattt tcgttgtctt cctttttcat
     5821 ggtgtaatat gcgccatact tattgatatt ttcaaaataa tctaaccatt cacttgaaac
     5881 agtcttggct tttttcgttt cgataaattt cgcatacgtt agttctaaag cctggtattc
     5941 agaaggttta atagtgcttg gtttgacacc tatttcaaag catagtaagg cttggaaaaa
     6001 ccattttgca ttttgctttt tcagttcttc cagtgttcga tagtacgtcg caataagatt
     6061 catttcgaca cctgattcaa tcatttcttc cacagctacc gacaattttt ctttataatg
     6121 tttttttgga acacgtgttt cgttgtatag gaacgtcata tcctccattt tttgtgccat
     6181 tttatccaaa ggtaatttga caaattcgtc atagtaattt tgagccatat ttttttctcc
     6241 tttttagttc tctaatttgg ttaaccaata ttcgttttca taagtttcca agttttcttg
     6301 ataatgcagt tcacatcctg caatcttcac taggtcttca aatagcatag tcggtgcatc
     6361 ctctactaaa ggggtttcta tcgtccagta ccgcggaata agtaaagctt ctgctacttt
     6421 ttcagaaatg acttctttgt ccaccaaaat ttgaacgact tcttttacag tcaataattt
     6481 gtgttgtgtt ttcatcagcg tttctttcct ttctcctcaa tcaaaatata atcgccataa
     6541 gacaaggtat attttttccc gcatgttgga cattgataca aacaatagct tttgatacat
     6601 ttcgctcttt ctccacaatc gcattcgaca gtatgtggtg catgatagtt tgggaataat
     6661 tcgctgaagg gaacacccaa ataatcaaat ttctttcgca tgattgattc tgcctcctct
     6721 taggaaaggt tttacttcct ttcgttagtt ctttctttat tgatttgttg gccagaatga
     6781 atcaacgaaa agcgacaagg acaagggcgc gcagcgtgcc atttaccctt gtccttgtcg
     6841 ctttttggtg ataaaaggca acttgttgcc ggtctggcaa atcaataaag agatagcgaa
     6901 caggaacgaa ccttttggtg agaggaagct tttcctattt tgtcttacaa agcaagaccc
     6961 cttatcttag tgaacgtgca acgttcactt cctttcttct cgaacggcgg tggcggttgg
     7021 agtgagagaa aggtttttca attgagaagg cacttgtgcc ttcctaaaca ggcctttccc
     7081 tttcttcctg tttctatgta aaacaaaaag agctgcagtc ctacattagg attgcagctc
     7141 ttcttttatt tatatgccca ttgatcgcgt aaaatgatca gggtttctaa tttgcatcga
     7201 ttcaatgact aattgattcc gtttattgaa atgacctgtg acggcaatat tgtattttcc
     7261 atcatccata attaacaatt tatcgataat ttcaatatct gcaactacac aattaatcga
     7321 ttcatgaatt gtttgtaaag taaagcgaac caataatggt tgtgttttta ttactcggat
     7381 tttatcaagc aaaccaacat atttgttcat cacgatcacc tcgctaaaat tatagcaaaa
     7441 acagttaaaa ttgagaattt ttattcgatt ttagatatgc tataatagtc aagacagtaa
     7501 agtttgaaga cggtggcgac ttctggaaag gtggtggtgc ttatgaatgt aagtgctaaa
     7561 atctataaaa ggagtaagcc ttttgtccgc atatgagaca attcaaacca ttcttggttt
     7621 tggtatgttt accattgctt tgattagttt gattgtaaat atgcttaaaa atgacaaaaa
     7681 gaaataaccg tctactttag caggtcagcg gttatttcca aataactaat attctgccac
     7741 cgttctttaa tcggtgctgt taatgagggg ttgttagcgc aactcctctt tttcatgttc
     7801 attatagcaa tatttcgcaa gaagttcaac gatttcccgt taactctaca tataaagcgg
     7861 tagaatagcc gtaagtataa cagctgtcgc tgctactgtg actttagtca atcgccaaaa
     7921 ggaattatcc tttcctgctc tttttaaaag ataagaacag acaagataca cgaatacaag
     7981 aaatagtgat cctttcgcaa ttctatttac caaaggattt tcagtgacgc ccaaagaaat
     8041 cccgtctata aatgcaatta cgggtatcaa tagatccatc gtcagactaa ttttttttgc
     8101 catgcttact ccttctttct caaactaaaa cggtagatca tcatcgctaa tatcgattga
     8161 ttgtccacca aatggattct gtgaagcgtt caatccattg ttgttaggcg tcgctgtatc
     8221 cgttgttcaa aggtggcggt gcttggactg gtggacgatt gatcagcgtg cgcacggctc
     8281 tctgtcgctg ctttattttc taatagttga aagttatctg ctacaacttc agtcacatat
     8341 acacgttgtc cttgctggtt gtcgtaagtc cgcgtttgaa tccgcccaac tacaccaatc
     8401 aagctacctt ttttagctaa tgtcgccatt gtttctgctg gtttgcgcca aatcacgcag
     8461 ttaataaaat cggcttctcg attgccgttt tgattcgtga aatttcggtt gacggctagg
     8521 gtaaaagtcg ctactgcagt accacttgcg gtataacgta aatcaggatc tttggttaag
     8581 cgtcctacta atgtcacatt attaatcatc tttatctctc catttctcta ttcatctgtt
     8641 ggtgtctttg ataagctagt ttttgctctt tttcttcgac gtgaatcact ttttccatca
     8701 aattactaat cgaatccatt gtctcatcgt acgtgatcga tgcagcataa ggattgcttt
     8761 tttgatattt cttccaggta tcttttagct cgtgactatt tttcagtaca tccatatcct
     8821 tttcatagta tatctttaac ggatcagttt ttcctcgacg tgtaaatgta tttctcacac
     8881 tatctttcaa ggtcttctca tcgatcttct ccattttagg aatagtatag agatcataaa
     8941 aatcttttcc tctcgaatta tctgttaaaa aatcaaaggt tgcacttaat ttttcagcaa
     9001 taatttgttc tgtaggatag gattccaaag gaatcgttcg atcctcaaac atcaatttat
     9061 gactgtgagt gatcgttgca ggatagatcg agtcacctgt agtcacatct accttaaaat
     9121 ctggccgcat gttttccaaa tgtgccagca cacgtaagct aaatcctgga tagtaatcgg
     9181 cttctctcgt ttctttcatt ccttgaatct caaattgaat gtcttctttc gtcggcgttg
     9241 aaaaaatttc gtttagaacc gttgttaatg tttctttggt tactttcatt tcccgtaaag
     9301 tcgtatcaat atctttggtt gtgcgatttt caatgccata ctttgagcca atcaagaagc
     9361 cgccttttaa cacaaaattt tcacgataag gagactctga gattttttcc aagaattttt
     9421 ccatcataaa atgtcttaaa acaagctgcg gatcaacttc tttttctcta gaaatattct
     9481 tcacttttgc ttttaactgc gtaggtgtca tgtatcttct cctttaattt aaagccataa
     9541 tataagaacg catcgttttg tctgttggta aaattcgtcg atactcattc aattttctaa
     9601 gattttttct gtccgactcc atatagtttt tgattgcttt tactttgatt tcatcctcaa
     9661 cggtatacca gggattccaa atgtcacata aagttctttc tggatcataa gctataatag
     9721 gattcccata gcgacttttt atttcaatct tacctaattc ataccattcc gcttttgtat
     9781 agtgtagctc aactgcgaat tccttcaatc ctttttcaga aacgtgataa ccgtaaggta
     9841 cagtcagatc gatctgtcta gggatcatat ctgttaaatc atacaaatct aacgcggtaa
     9901 tatgtgaaat aatgccgcgt tcatattttt tttgcgccat aaacaattca tcaggaaatt
     9961 gaccaggtaa ggtgaaaaaa cctggatact ctcgatccag ctcccctcga tctaccatac
    10021 gtttgatcgt tacaggagaa atgcccgctt ttttggcatc cttcaaagcc aaagttccat
    10081 taaattcttc tagcagcttg tataccattg cttttgtcat tttctccctc ctttgtatac
    10141 gtataaactt ataaaaacag tataacataa gtttatacgt atacaatagt taggaagatt
    10201 ttatcgatct aattcgattt ctttttgaat cctctgctgg ttcttcaatt gctgcagtct
    10261 tcgtttgctg gcacgcattt tttcctgttc tgaacgaatt ttggcttttt gattttcgat
    10321 ttcttgttgt ttgtgtgtgg tattacgcat aagattaacc ctccattatt ttgcagtgcg
    10381 gcgtgttctt ttcttgaagt tcgttttttt cttttcagaa aattcaaagc ctgtagtgaa
    10441 gtcttctttt aaaacaatcg ttgcttcaaa tgaacccttc tgacctttta aaatcacagg
    10501 cttcagacgt acaagctgcc accttttgat agagtaagac ttcttgttcc tggcattttg
    10561 gacaaattcc taaatgacgc gcggcttctt gcttttcact ggctgctttc acttcttgta
    10621 acgtcgatcc aaaatcagtg gattggattt gccctggtac gacttccaat aagtgagaga
    10681 caaatttttg gatattggct agaaaagtgg tttgtgttcc ttttcgttca ccgatcttta
    10741 acaagtagct ttcccatttg gcggtcattt cggcgctcgt aagcagcggc tctttttcca
    10801 cggcttggca aagcaaaatc cctttattcg tcaccacgag cttatttttc tctaccttga
    10861 tatattcctt ttgtttcaag gtttcaataa tattcgcccg tgtcgcttct gtaccgattc
    10921 cttccacttc tttcaatatg gattgcgctt cttcactatc cacggttttc ccagctgttt
    10981 tcattgcagt gatcaacgta ccttcggtat aaggttgtgg tggcttggtt tctttttcaa
    11041 gaacttgtag atccaccgtc accatttctc cttttgtcac tttaggaagt agcggctctt
    11101 cttccttctc ttttccgtcg ctctcttttc caaataactt cttccaacct agatctagcg
    11161 gcaccttttc gattgccttt aactgaagct gctgaacttg cgtcaagatc gttgtttctt
    11221 cataggtata cttttccgca aacatggcca ccgttgtttt taacacttgt tcataaatag
    11281 cctgctgcag tggacttagt tgttcaaaag ccgctgctgt cataaccgtt tttgtaggaa
    11341 tgatcgcgta atgttcctgg actttcttat tattcacata ccgactattc ggtgtatgaa
    11401 taggagtcgg aatcgcttcg gcttttaaaa aatgcttgta ttcgtctaag tgatccaaca
    11461 gatacgcata ttccccttcc gtaatataag gggtatccgt tcgcggatag cttagatatt
    11521 ttccttcata caacccttgc atggcttcca aagtatcttt ggcactagct ttcatgagtt
    11581 gattcatttt agactgaagg ctcgataaag aaaaaagacg tgggctagcc gcactttttt
    11641 tctctgtttc gacttgaagg atcgtacctg gctgttttcc taattgaaca cctttttctt
    11701 ttaaataggc ttcaaatgct tcttgtgtcg cttgggtttc atttggatca agcttcgcat
    11761 caaatgcgcc attttgagcg attacttggg cttttccttc aaaatatggt tctttttgga
    11821 agtttctaat cttttcctgc agctgataaa tcatgtaaag cgttggggtt tggactctgc
    11881 ctaaggaaaa acttcctggt atgccttttt gctgcaagag taaactatac agtgggctgc
    11941 cgttcatgcc gatcaaccaa tcagcgatct ggcgtgcttg tgcttcttca aatttgggat
    12001 aggtctcttc tgcgtctcgt aattgctgga atcactgata aatggcttct ttttctaagg
    12061 agttgatcca aagtctttta aatatctttc catgatcggc gcctgcttgt tgaatgatcg
    12121 tccaagctat cagttcccct tcacgatcgc tatctgtcgc aataataatg gtgtcggccg
    12181 attgaagttg ctgtttcacc accttgaatt gctttttctt atctttagga acatgatagt
    12241 gataatgtgt agggaaaata ggcaaatgct ctaatgacca ttgtttccaa ttttcatcgt
    12301 acatatcggg cgaatctaac tcgactaaat gaccaaaacc atacgtgatc acagtttctc
    12361 ctggaaacaa ccgatcttgg atttcaaaat acccgtcttt tcgggtggct ttagagaagc
    12421 tatcggcata agctttcgct tgtgacggct tttctgctaa aataacggtt ttcatcatga
    12481 cgatttcact ccctttcttg ttgtttgcct tgttgaaaca acatcgcacg tttttgcttt
    12541 ctgatctctt gttggtggat ttcatgcttc aattgatcat tccaatcctt ttttacttca
    12601 ggtacaaatt catgttcatg atttttatag cctttgtagt attgttctgc tttataagag
    12661 aaaagaatct gagcattaat atcccctttt tcttgataaa aacgatcaaa atcgtacgta
    12721 ccatccacgc gttcacaacc ttttaattct tttgcgactt ctctacaaat atctttcaaa
    12781 ttaatagctt ctattgaatc gttaacagaa agctgctgac caaaagacgc atttactttg
    12841 tgagcggaaa ccagttggtt cgttaattgc agattgtttt ctgtcttgtg tatggccata
    12901 attgcttcag gttcaacttt catttctttt gcaacttctt cataaatgac tttgtattct
    12961 tttggcactt gccaatcatt agcgattcct cgttcacaac gtactttctg attcgtaaat
    13021 ggatcaactg cgcccattaa ttgttctttt tcgtagaaaa tatgtcctgc acggtcatta
    13081 tccacacaaa tctcaatgga ttgaggaaag gcttgtttct ttctcaattc agatactgcc
    13141 tcaccaaaat aatgactgat cacatggtgt tttaaccctt ccatagagac taaccacgta
    13201 tcgtttaact gatcacgatt cagtgcagca tagctaagta aatcaatact actctcaaaa
    13261 aattttaact gtttagggtc accgatcttt aggttaaatc catgattcgc tgtcgaattt
    13321 ttatctatat gcttatatgt gcctctctca ccaaaacgtt cataatcaat gaatgttcct
    13381 tgataactcg cccctactgt ctcttttgtc tgtggatctt tccataaaaa ggcgaccgca
    13441 tgatccaaac gatcgtcttg aactaagaaa ttttgccgtt catgttttgg ctttagttcg
    13501 gcgatcaatc cttctttttc tgcataatca atcaaccaat cgggcagttt tcgttcttcc
    13561 ttcaaataat tccttccttt ttctgtgagt ggttgataat caacggggtg atctttgtac
    13621 ttttctgtct caaaatgaag attttctact cgttctgttt tgtcttcatt ctttagaata
    13681 aaatctgtgg ctgctttaaa ttgttgctta gagtcttgaa tctctttgtc tatgaaaaaa
    13741 agtttagcta attcaataat gtcgccacta atattttgac tgttccaata aaaacgttgt
    13801 tttcttcgat caaaaacaaa tgaatcatgg tcttctaagc gatagtctcg tcctttattg
    13861 actacagcca ttccattgtt tcgagcaaaa tccactatat cgatctgttt acatcgatca
    13921 ataagactca tttttttctc ggtcattttc aaacctccca aaagaaaaaa gactatccgt
    13981 ttttcagata gtctttttta cttgttttat acgcgttctt tttgtgacaa ttgttgttca
    14041 taatagattt cattttttcg tttggccact tctgacactg tttcaagtga aaatcggaat
    14101 ggatttccta gcgcaaattc ttctgaaggt aacgccgttt gaatctcttt tccataactt
    14161 tttcctttta aaatcacttg attatgctgc gtatcttcaa taaacgccgc atccttcaaa
    14221 tgtaacgttc ggcccgtgtc ttcttccagg actttcccgt ctttcttgtc gatccaaagt
    14281 gagccaacga gcacaatatt cttttgttct gctgtttcta caacattttt ctcatcctgc
    14341 ttcacttctg ctggctggat ctctggttct ctttctctgt attgattgat ttcattagaa
    14401 atggtttgaa tgaattcatt tgcgacacga ctgacatcat tcatgatttt cccacgttcc
    14461 gcagcttcct tatcatccaa cgtctgacca ttcttcgtcc atgtcgcaat ataaggaata
    14521 gctttctccg aggtgtccat cccaaaatgc ttgcacacaa tataggaggt catttctgcc
    14581 tgtagttctt ttgttgcagt atctaattct gaaaatttgt gcattctagc atgggctaat
    14641 tcgtgaatac ttgtggctaa atgctgcgta ggagtatttc gagtattcat gacgatttct
    14701 tcttcgccag taaattcgtt acgaacgtac gccccacgtg cttgtcctaa ttctctttga
    14761 tagacttcac tttctgtcat atctcgaatt tgaattccaa tatttttggc taccgcttga
    14821 atcccaactt gaagttctgt atgccctgtt tgatccaatt gaaaatcaaa gacgcggttc
    14881 gggaagattt tcggatagtc ttcaggcttc aattgtgttt gtgtgatatc aaacactttt
    14941 tcaatcgtgt agtaaggttt tttgatcact tcgatttgac cactttcgat tcgtttttta
    15001 tcttctggtg tagcctctgt ttctcgaaca aagccacgtc ctttctcata aaatcctttg
    15061 accgtttttc tgacaaagat cttgttccct tgttcccctt ttttgatata gattccttct
    15121 tttttcaacg cggcacgacc taaacaagcc gttgcgcctg gaaattgttc ctcgatcaat
    15181 aattgatttc taaaggatcg ttctggaaaa cgagacataa aattcaaaag ttctttgacg
    15241 tcttcaggtg aatgagtatg ctttttcacc gcatcaagcg cctgttggga aagtgatttg
    15301 acttcttgct taatctcttc tgccgttcgt ttttgataac ccttttgttt tgtttttgtc
    15361 tctttaatct cattttgtac ttttgaaata ggttcatttt tgactgtttg tttttgttcc
    15421 ttctcaggta cttgttcgca caatctagct aactctaaat agatggggtg attactattg
    15481 acgccatccc ctagatccat tcgtatctga tctactactt ctccattctt cacttcttca
    15541 aaatagaatt tataatagcc tgcttctgcg gcgtattggt tatccagtgt tttgagttga
    15601 tcgatcaatt ctagtgttac gatctcccca ccataacttt tcaacccttt tgctggatca
    15661 ttttcgttga attctactcg atagaaatga tcggtttctt tctccttcac agaatcagta
    15721 ggtgtagctt cttcaagtaa ttcacgaaca gctgcctttt cttcttccga cgtggcttgg
    15781 tattttttag attcctcaaa tcccccgttt tcattgagtt ctaaagcgac ttttcgatag
    15841 cctttcagaa aatagatagt tggattcttt ttccattgcg tcactcgtcc gctgtcaatg
    15901 atcgcttgta cttcaggaag aatattttct tttcctttct ctgatttttc tttcagctgt
    15961 tctaatgttt ccaattttcg ttggtatttt cggatcgtgg cagctgaaaa agtcgattca
    16021 cctttttcaa agcgttcgat ttcttctttg attcttggaa tattttcgat cgtcatatcc
    16081 aggccaccgt acttgttata acccatttcc tttagctctt cttgtctttc aagtttttct
    16141 acctgttttt cttgttgctt gatctcttct ctcagacttt ggactttatt ttcgattcgt
    16201 tcttgttgcc taaaccaaga atgcccgttt ctcttatcgt tcattggttg accgttggtc
    16261 tttctttggt gttcaaacat cttttcgatt tgatcattta attcattttg caatcgtact
    16321 aattttcttt tggattttcc caaacgacta ttgtctagtg gttctttttc ttctgccatg
    16381 tc
//
LOCUS       DAFEHS010000062        15913 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFEHS010000062
VERSION     DAFEHS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.064"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            292..1215
                     /gene="truB"
                     /locus_tag="LP327_002051"
     CDS             292..1215
                     /gene="truB"
                     /locus_tag="LP327_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBL1726154.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1219..2160
                     /gene="ribF"
                     /locus_tag="LP327_002052"
     CDS             1219..2160
                     /gene="ribF"
                     /locus_tag="LP327_002052"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBL1726155.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2512..3297
                     /locus_tag="LP327_002053"
     CDS             2512..3297
                     /locus_tag="LP327_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL1726156.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3310..4161
                     /locus_tag="LP327_002054"
     CDS             3310..4161
                     /locus_tag="LP327_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBL1726157.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4285..4737)
                     /locus_tag="LP327_002055"
     CDS             complement(4285..4737)
                     /locus_tag="LP327_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726158.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(5202..5570)
                     /locus_tag="LP327_002056"
     CDS             complement(5202..5570)
                     /locus_tag="LP327_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBL1726159.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5720..6202
                     /locus_tag="LP327_002057"
     CDS             5720..6202
                     /locus_tag="LP327_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBL1726160.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6239..7249
                     /locus_tag="LP327_002058"
     CDS             6239..7249
                     /locus_tag="LP327_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBL1726161.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7364..8077)
                     /locus_tag="LP327_002059"
     CDS             complement(7364..8077)
                     /locus_tag="LP327_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726162.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(8267..8857)
                     /locus_tag="LP327_002060"
     CDS             complement(8267..8857)
                     /locus_tag="LP327_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBL1726163.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            9341..9568
                     /locus_tag="LP327_002061"
     CDS             9341..9568
                     /locus_tag="LP327_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL1726164.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            9584..10750
                     /locus_tag="LP327_002062"
     CDS             9584..10750
                     /locus_tag="LP327_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBL1726165.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            10984..12027
                     /gene="hrcA"
                     /locus_tag="LP327_002063"
     CDS             10984..12027
                     /gene="hrcA"
                     /locus_tag="LP327_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL1726166.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            12121..12684
                     /gene="grpE"
                     /locus_tag="LP327_002064"
     CDS             12121..12684
                     /gene="grpE"
                     /locus_tag="LP327_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL1726167.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            12735..14564
                     /gene="dnaK"
                     /locus_tag="LP327_002065"
     CDS             12735..14564
                     /gene="dnaK"
                     /locus_tag="LP327_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL1726168.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            14715..15881
                     /gene="dnaJ"
                     /locus_tag="LP327_002066"
     CDS             14715..15881
                     /gene="dnaJ"
                     /locus_tag="LP327_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL1726169.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         5383 a         2656 c         3362 g         4512 t
ORIGIN      
        1 aaaaagactg gaagaatggc cagtcttttt tttcgttttt gggaagtgaa aaccaaacta
       61 tttacctgta agccacccat aaatccaatc aaatggtgca aagaaaaaat cttccattct
      121 attccctcct ttaaaaaagc ataacatgat ttctatttac gtgagcatga attttgttac
      181 gtttttattg ccgaaaaata aaaaccgttc attatgatgt caaattcgtc agtaattgat
      241 gcaatgtgtt ataataattc agttaaatca aaaatgaaat ggagaaaaga catggacggg
      301 ttattacctt tatggaaaga acgcggaatg acaagtcatg attgcgtatt caaattaaga
      361 aaaatcttac atacgaaaaa aatcggacat gggggtacgc ttgacccaga cgtagacggt
      421 gtgttgccca tttgtattgg aaaagccaca aaagttatcg aatatttgac agattctggg
      481 aaaatctatg taggcgagat aactctaggt ttcagtacaa cgacggaaga tcgatccgga
      541 gaacgggtag aaacaaaacc agtagatatg tctttatctg aaaaaaaaat agatgaagtg
      601 atggcttcat ttattggtga gatcacccag ataccaccaa tgtactcagc ggtaaaagtc
      661 aacggtaagc ggttatatga atatgcccgc aataatgaaa cagtggagcg tcctgttaga
      721 aaggctcaga tttaccgttt tatccggaca agtgaattga caagagacga agtgacagga
      781 accatttctt ggcgcttcga agtagaatgc gggaaaggaa cctatgtccg cacattagct
      841 gtagatacag gcaagaaact tggttaccct gcgcacatgt ctgatctgac ccgaacagca
      901 agtgccggta tgtcccagaa ggatgctctg acattagaag aagtagctca agctgtgaaa
      961 aatgggacaa tcgatgattg tttgctgcca atagaaaaag gagtggaacg gttcaaacga
     1021 gtagatatta ctgatgaagt atggcagaaa gtaaaaaacg gtatgagact tgattatact
     1081 gtatttgcgc tcccagaaat gccgtcagaa gaaattgctt tgttttataa aggaaaagtt
     1141 gtcagtattt atcagccaaa cccgaaagaa aaaaacaaat taaaacctag taaagtattg
     1201 agaaatgagg aataacgaat gaatatcatc aagatcagac atccttacca atcagaacaa
     1261 attccagaag acgatgtcgt tttagtgctg ggatttttcg acggcgtgca tatcggacac
     1321 cagaaagtca taaagacagg tatggaaatt gcacaaaaag aaggattgaa attagcattg
     1381 atgactttca atcagcatcc atctattgtc tttaaaaaga tcgatcctag cagcgtgaaa
     1441 tatttgacta gccttaagca aaaagaagaa aaaatggccg ctttgggtat agattattta
     1501 tatgaagttg attttacttc ttcttttgct catttagcac cgcaggaatt tgttgatcaa
     1561 tacatcgtag ggttacatgc aaaatatgct gtttccggtt ttgattatac gtatggacca
     1621 aaagacattg cagatgtcgc tcattttcct gaatatgcca aaggaagatt caaaatcgtc
     1681 acagtttcta aagaagaaga acaaggtgaa aaaatcagct ctacccgtat tcgtggatta
     1741 ctagacagcg gacaagtaaa agaagcagca aaactattag gttgcacata tgaggtagaa
     1801 ggggtagttg tccatggcga tgcacgaggc agactactag gattccctac agcaaatgtc
     1861 aaagtaaaga gcacggttca tttgcctaaa gaaggtgttt atgtgtcgga aataaaaatt
     1921 ggggaaacat ggtatccagc catgggttca attggacata atgatacatt cggtgaagga
     1981 cgggagctga ctgtagagtt gtatattttg gactttaatc aagatatcta tggtgaacat
     2041 gtcgcggtac gctggaatca atttttaaga gatcaagtga aattttctag tgtagaggaa
     2101 ttgattgaac aactcaagga ggatgaacaa gcaacagctg aatatttttc tcagcaataa
     2161 aacattatgc ataaaatctc ttgtttatgt ataactagca attacaataa aatgggtgat
     2221 aaataagccc aggaaagggg ctgtgacaaa agtcttgtca cagccttttt ttccaaataa
     2281 atggtttttc agaagtagct tcttcggaaa taagccgaaa tttcttgaag ctgaccactt
     2341 ctgtcacaac ctctttcttt atagaaataa aaggacacta gcaattcatg aaaacgattg
     2401 ctagagcgta tcatttgctt tagactatat atcttggtgt acactcactt taaggcggaa
     2461 ataaaagtta tccgtaataa caggtttagg tatgaaaaag gagtgggtac tatgtacaag
     2521 gaattagaaa acaaagtcgc agtagtaact ggtggctcaa aggggattgg aacagcgatt
     2581 gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca actataactc ggataaagaa
     2641 ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg gaaaagcagt ctctgtacaa
     2701 gcagatgtca gttctgaaga aggaatacag cgtttacttg atacggcttt agaaaatttt
     2761 gggacactgg acgtttgggt taataatgct gggatggaaa atcagcatcc aacccatgaa
     2821 cttagccttg aagattggga aagagtgtta aaagtcaatt tgactggcgt ttttatcgga
     2881 acgaaagcgg ctttaaatta ctttacagag aataataaaa aaggaaatat catcaatatg
     2941 tcttctgttc atgaacagat cccatggcca acgtttgctc attacgctgc ttcaaaaggc
     3001 ggggtaaaat tatttaccga aacggtagca atggagtatg caccgaaggg aatcagaatc
     3061 aatagtatag gccctggtgc tatccgtaca cctattaatg cggcaaaatt tgatgatcct
     3121 gaacaaaaag aactcttgga aagtatgatc ccactaggac gcgtaggtga accaaaagaa
     3181 atagcggcga cagctgcatg gttggcttca gacgaatcca gttatgttac aggtattaca
     3241 ctatttgcag atggtgggat gactttatat ccttctttcc aaggtggaaa aggataaggg
     3301 tgaataaaaa tgggtttatt gattgcattg atcccggcag ttggctgggg gatccagcca
     3361 ctgattttga aaaagattgg tggcagacca acaaatgaga tattaggaac tggtattgga
     3421 gcggtgcttg tcggcttact cgttcaatta ttcgcttctc cagctggtat ttcagtgact
     3481 acatttttga ttagtttact ttctggtgca ttttgggtta ttggacaaac aggacagtat
     3541 acatctttca acttgattgg tgtttctaaa acgatgccaa tttcgactgc attacagtta
     3601 gtcggaacct cgttgattgg tgtcttcgct tttggagaat ggtctggaag tatggggaaa
     3661 atcattggag cagcagcaat cattcttctt gtatttggtt ctgctttaac ggcaatcagt
     3721 gatgatcctg gaaaaaaagg cggtatcaca aaaggaatta gtctattagc atcaacaagt
     3781 attggttatt gggtatacag tgcattgcct aaactagttg atgcaagcgg actttccatt
     3841 ttctttccgc aaatgatcgg tgtcttttta ggggcagtga tttatgtgat tttccgtaaa
     3901 ccaaccgcat tttctgaaga caagagttgg aaagcaacga tcgttggagc catctttagt
     3961 gtgtcagcat tggcttatat tttttcagca aatgaaaatg gggttgctac tgcatatatc
     4021 attacacagc taaacgtagt tatctctaca ttaggcggta tgtacatcct gcatgaaaat
     4081 aagacaccaa aagaaaaaaa attaacgatt atcggattat tgctgatcgt agtaggaagc
     4141 atggtcacgg tctttatttg aggataaaga tggtaaaatt tcatttatta aaaaaatatt
     4201 tgtttgaacc aaacagtaag tacttataaa aaaatagcgc tagaatttcc aacatctctg
     4261 gaatttctag cgctttttaa ttgtttaaga tacaagggaa atatctgtct ggatcaataa
     4321 tttttcattg atccattgac taaagtttac gacataattg aagttaccac cgattagtat
     4381 ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga cggtcttttt tatttgcgta
     4441 aacatcgtta tggtactgga ctaattgttc tcggaattga ttgattgctg taaaatcacg
     4501 atattgaagg ttgaaacgca tcgtgattgg tgcatttagc atgaatcgct gcatttcttt
     4561 tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa agattacgtt cagaaaattt
     4621 ccattgcccg ccaacctttg ttccttttag ttttccttca tgaagataat ttctgataga
     4681 acgtgtagta acacctaatt gttcggctaa ttcatttact gagtaaaaat ctttcaatgt
     4741 tcaccctcct atgtgaatgg ttctactgac ttagttgata tttgcttaat cacgaaatgt
     4801 agaaagaacg attttaatta aggtcattgt aaaaatcaag tcagaaaatt ctaactttta
     4861 tttctctaaa gaaattcgtt ctctcctaaa gtgatattat atttaaatat acctgaaaat
     4921 aatgggcagt attatgaaag atcactgata ttttctcgta ttttttcctt attcaaaagt
     4981 tttgcaggaa aattgctgaa aaaataattt tactaagtag aaaattcaaa gccattcaat
     5041 cagtgatttt tttcagcaca agaaagactc cttataaaaa tagcagatac tttgtcaatt
     5101 aagtgtttaa ccagggatga taaaaaggat aggattgaac aaaacctgta taaggtgatg
     5161 aatagtttcc tcgccagtaa aaggtttatt cgtataaagc tttatcgcca aaatttatca
     5221 tgaccttttt tatcttcaat attaaatgaa atcaattgtt caatcaattc ccagttgatt
     5281 acacttttcc aaggaacttt gatgacgtta tccgtatata cgtagcctgc ttcttggata
     5341 tttttttcaa gggcacgaat cgctgctttt tccggtgcga tagaaaaatg atttttagca
     5401 aaactaaatg caataataaa ggtaccatgg tcagtaaaca taggttggtt ccattttaca
     5461 gttgtgtcta gctggggata cgtttctgtt atcttagcaa aaaggtcttt gatcttttgt
     5521 tgatgttctt tttctttgat ttgattgatg atcggctgaa atttttccat tctaaaacgc
     5581 ctccattaag tggtttgatt tattttacca gaaaaacaat agttatgtat aaaaaacgaa
     5641 caaaacacaa gtaatgttgt aaccgctata tttatatgat aagatgtctc aaataataaa
     5701 atagaaaaga gacataaaaa tgattgactt ggcaaagaaa ttcttacacg aaaatatata
     5761 ttttgagttg tatcatcatc gagccattta tacaaatgaa gatgctttgg taataaagaa
     5821 agaacaagga tttacaggta cagagaccaa aagcttattc ttaaaagata aaaaagataa
     5881 taactacgta tatctgactt ttactacgaa acaaacggat ttcaaagcat tagggaagat
     5941 cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg gaagaacaaa cgggacaaaa
     6001 gcagggcgct gtttctccgt ttggttatga aaaacagatt ccgattatta ttgatgaaga
     6061 gctcttatca aatgaaaaac ttgtttttgc accaggtaga ccagatcaaa cgatggtcat
     6121 ccaaacggtt gacttaaaca aaatcatcca gctacttgag atgccgatat ttttgatgcc
     6181 ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat acttggaggt gaagcaacat
     6241 gataagttat ccattattag aaagaaatgc aacgattggg gttactgcac catcctcagg
     6301 cgtatcagag gaacaccatt cgttactcga atcagcttta aagagaatgg aagaaaaatc
     6361 tttccaagta gttactggaa agacgctttg gacgcaagat aaggaaaaat caggtccagc
     6421 aagaaaacga gcagatgaat tcaatcaaat gatgcaagat gaaaatatcc aaatgatctt
     6481 tccgacttgg ggaggagaat tattgattga gattctcgat tttgtcgatt ttgatgctct
     6541 acaaccaaaa tggatagtag gttattcaga tattagtacg ttgctacttg ctgtcacatt
     6601 aaaaacaggg atagccactg cacatggaac taaccttatt gatttaagag gagagtactt
     6661 ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca agagaaggag aatcgattct
     6721 acagcaatct tctgaaaagt atcaaaaagt gtggccccat gagcattcat catcttctgt
     6781 tttccatctc actgaaccaa caaattggca atcagtcagc ggagatgcag taaggatgaa
     6841 gggacgatta ttaggtggtt gtattgatac gatcaggcat ttaatcggta ctccttatgg
     6901 agatgtccaa tcttttaaaa gaaagtataa tgatggaaag ccgatcttgt ggtttctaga
     6961 aaactgtgag ctatcgtcag ttgatttaag aagatcactt gttcagatga aactggcagg
     7021 ctggtttgat ggcagctcgg ggatcatgtt cggtagaagt gcggcagagg atttgttaga
     7081 ttataaaatg aaggatgtat acgaagatct gtcaaaggaa ttaaaactac cgatcttgta
     7141 tgatattgat tgtggtcatg taccgccaca actgacattg attaatggtg cctatgcaga
     7201 agtagcggta gaagatagaa aagcgactgt tttgcaaacg tttatttaaa aaattttttc
     7261 ctgccttgga gtctatcaaa gaccaataaa tctaaataga aaagaaagtg aaagaagctg
     7321 acaaaatgct gtcagcttct ttttgtataa catattagaa agtctaatta tctataactg
     7381 tcacatcgat taactgtttc acgtcattgg gatttcctgt aggaatagct actacagatg
     7441 cattatcaat gatttcttga gttgtttctc taatgaacgc tggataagat gtttgatctg
     7501 caggcatttg ccaatgtgaa ggaactggat aactagtgat tgtcccgtca tggttacttg
     7561 aatacactaa aactccctgg taactgtatt tcccagtgag cataaccaca tctttcggat
     7621 agactgcact ccctgcatca taaggggcga taggtgttcc tgcaggataa tgaataacat
     7681 gtaattcgaa atcacccgat gtatctctaa aagtattacc tctaatagct agccaaacac
     7741 gtgcgtactc aatttgtaga tcagaatatc ctgctaacgg atctattgac tgagaagact
     7801 gtgaggaggt tgtgtctatt gttgaagaag aaatatttgt ctcaactgta ctactagatg
     7861 tagaagaagt attgggctct gagactttac tgcttgttga agatgtattc gtttcagtag
     7921 aagctgattt ctgagtactt gatttttcag ctgaactggt ttgtctgatt gaacttttgt
     7981 ctgtggtcgc agatggatca ctattttctt tactaggaga cgtacatcct acaaggaata
     8041 aagagaccaa caataaaata aaacctaact ttttcatata aaatacctca ttattttttt
     8101 aataccagta tagcataatc ctgaattttt gaatccgtaa aaccttgtct atgaatataa
     8161 atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataatc atcagtaaaa
     8221 ttacttgtca cactattgat aaggagtatt taattgaaga gaatgattag gagaaaggat
     8281 ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc gttttttctc
     8341 ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat tctataggag
     8401 ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct ataaaatctt
     8461 ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg gtgtgtttgt
     8521 aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata gcagctactg
     8581 gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt tttatgtccc
     8641 atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt tcgattaagt
     8701 aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata gaagcagttt
     8761 tgttttgcca tttctcattt tggtttagtg tgctagtaat ataagcacct tcccaatcgg
     8821 catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc tttactatct
     8881 ataacaagga caacagtttt aacctacaga cattcattaa aattgggtag cgacagcgaa
     8941 aacaacaatt cttaagtttt agacagagta tacagatagt caacttaatt aaatacgaac
     9001 taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt tgcatacatg
     9061 gatgacaaaa gaaccaattc ccacttgctt gagtacaata ttaaaagaaa ctggaaggca
     9121 aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg aaaacaaccc
     9181 tagagatata taactaggta accagtttaa tgaaagaaaa aatcatccaa gaagtcgatt
     9241 cttggatttt ttagtggatt tgttcgccaa atacgtttag tatgcgtata tgagaggttg
     9301 taagcaaagc gttgtggtct gagcacttaa ggaggaatct gtgaaaatag cttttcatat
     9361 ttttgcagat tttgacttaa tgccgaaggt cagctttgcg attaccgttt atcgagaggc
     9421 tgtaaaagca ccgtgtagct ccgatgacaa taagcaagca tttttagagc taggtttcct
     9481 tgattctaaa tatgcggctt atttcaatga gttggtgctt gaacgcgatt ttaagaaggt
     9541 cgaaaacagg cacgactttt cgacgtaagg agaaagaatt tcaatgatag aaacaataaa
     9601 tcccgcagaa aacaaggttt ctgcgtacat tcatatccca ttttgcgagc atatctgcta
     9661 ttactgtgat tttaataaag tttttttaga agggcagcca gtagatgagt atgtggaaat
     9721 gctgctgaat gaaatgaaaa taatgctaga aagtcatccg acagcgaatt tagaaacact
     9781 ttacgtaggg ggcgggacac caacttcttt atctgctaaa caacttgatc gactgttatc
     9841 aggtgctaga gagatcctgc cttttcaaaa tggtaatgag tttactgtag aagcgaatcc
     9901 aggtgatttg acccgtgaaa aacttcaagt aatgaaaaac tatggcgtca atcgtttatc
     9961 catgggagtg cagacatttg ataatcgctt attgaaaaaa attggacgta aacacaccgc
    10021 agaagatgtg tatcaaacta tgcaattttt agaagcagaa aattttacca atgtaagcat
    10081 cgatttgatt tatgcattac cgggacaaac actagaagga tatctggata ctttggaaaa
    10141 agctttggct ttggatctgc ctcactattc tttgtactca ttgatcctcg aaaacaaaac
    10201 aatgttcatg aactgggtac gacaaggaag actcgaactg cctgatcagg aaacggaaac
    10261 cagaatgttt gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc aatatgaaat
    10321 aagtaacttt gggctctccg gacatgaatc tcaacataac ctcatgtact ggaacaatga
    10381 ccactatttt gggtttggag ctggtgctag cggttatctt ggagatattc gttaccgcaa
    10441 caaaggacca atccaacact atttacgccc attacgagca aatgaacgcc cggtattgga
    10501 agaagaaaaa ttgacacgaa aaaatcaaat agaagaagaa atgttcttag gactaagaaa
    10561 aaaaacaggg attactaaaa aacatttcta tgatcgctat caaaaaacaa ttgaatcggt
    10621 gtacggtgat gtgctaaggg aattagagca agaagggtta cttgttaatg aagaagaccg
    10681 tgtttatttg acaccaagag ggaccttttt ggggaacaat gtgtttgagc gctttttgtt
    10741 tgaaaaatag tttaaagaat atttgttttt tatctgaatt taaataaaaa aagttcaaaa
    10801 atacaataaa ggactattga aacgttgata taactggatt tacatcaatt atttagaatt
    10861 agcactcttg tgtttagagt gctaaaaaat ggagtctttc tcttgacagg agaagggctt
    10921 cttgctatat ttataagtgg attagcactt agaatatatg agtgctaatg agaggtgagg
    10981 aagatgttaa caaagcgaca aaacgacatt ttacgtctga tcatccaaca ctatacgagt
    11041 agtggcgtgc ccgttggttc caaaacattg atgtctgagg gagtcaaggc cagctcagca
    11101 acgattcgca atgatatgaa agcattagaa gatgaaggat tgctgttgaa gactcactct
    11161 tcttctgggc ggattccatc tgctttaggc tatcgttatt acgtggatca tttgttaaaa
    11221 cctgctcgcg tagcagaaga tgatttgctg ctgattcgtc agtctctagg cagagaattt
    11281 catgaaatca atgagattat taagcaatct gctgagatat tatctgagct gacaagctat
    11341 acgacttttt cattgggacc tgaaataaaa gaccgccgac tgactggctt tcgtatcgta
    11401 cccttgaata atcgacagat tattgctatc gtcgtaacag atatggggaa tgtagaaagc
    11461 caagtgttca ctcttcccga atatatggga agtcaggaac tagaaaaaat ggtacagatt
    11521 gtaaacgacc gtctagtcgg agatcctctg atgacggtgt accataaact tcgtacggag
    11581 attccgatga ttcttcataa atatttccaa acgacagaag ggatgtctca gttatttgac
    11641 accgttcttg gtcatgtttt tgaagataag gtttatgtca gtggacaaat gaatctcttg
    11701 gattatgagc cccgtcaaga tcttgatcag ttcaaatcga tgttttcttt tatgaaagac
    11761 tcagacggac ttacacaaat gatcactccg atggatagca atattcatat caagattggt
    11821 gctgaattag gcaatgattt attgcagaat atgagtatga ttcaagcaag ttacgagata
    11881 gatggtcatg gaagaggtac aatagccttg ttagggccta ctagtatgcc ttattctcgg
    11941 atgttcggtc tagtcgatgt ttatcgaaga gaactagcag ctaaacttgc tgattactac
    12001 cgttcgcttg acttgtcaaa ctcgtaatgt gaataacgaa gctatttgca gctgatcgtt
    12061 ctctctttct tagtaagagc aatcaaacag ctgactgaat aatgaaagga agactgtatc
    12121 gtgaaagaaa atcaagaaga actagataaa gaaatgacag atgctcagcc agaaccggaa
    12181 atcgatgtcg aagctgcaga agattcgggt atttcagaag ctgaagcgga agaattcgaa
    12241 acagcgaaat taaaagctga gttagaagaa atggaagata aatacttgcg ggctcgtgct
    12301 gaaatcgcaa atatggcaaa tcgaggaaaa aatgagcgtg agcaattaca aaaataccgt
    12361 tcacaagatt tagccaaaaa attacttcca tcaattgaca acctggaacg tgcattagcc
    12421 acagaagtgt cagatgatca aggggcaggt ttgaaaaaag gcgtggaaat ggtcttggaa
    12481 agtttgcgta acgcactgga agaagaagga attgaaaaaa tccctgctaa aggagaagcc
    12541 tttgatccta atcttcatca agcagtacaa actgttccag cgactgaaga tacaccagcc
    12601 gatacgattg tagaagtact acaagaaggc tacaaattac atgatcgcgt gttaagacca
    12661 accatggtca ttgttgcaca ataattgtgc ggatataaaa cttacacttt caattaaaga
    12721 aggagacgtt aattatgagt aaaattatcg gtattgactt aggtacgact aactctgcag
    12781 tagcagtatt ggaaggaaac gaagcaaaaa tcatcgctaa cccagaagga aaccgtacaa
    12841 caccttctgt tgtatcattc aaaaatggag aaatccaagt aggggaagtt gcaaaacgtc
    12901 aagctgtgac aaacccaaat acgatttcat caatcaaacg ccatatcggt gaagcaggtt
    12961 acaaagtaga tgtagaaggt aaatcatata caccacaaga aatctcagca atgatccttc
    13021 aatatatcaa aggattcgca gaagactatt taggtgaaaa agtcgaaaaa gcagttatca
    13081 ctgtgccagc ttactttaat gatgcacaac gtcaagcaac aaaagatgct ggtaagatcg
    13141 ctggtctaga agtagaacgt atcgtcaatg aaccaactgc agctgctctt gcctatggtt
    13201 tagataaaac agatcgcgac gaaaaaatct tagtattcga ccttggtggc ggtacttttg
    13261 acgtatctat ccttgaacta ggagacggtg tctttgatgt attgtcaaca gctggcgaca
    13321 accatctagg tggggacgac tttgataaca aaatcatcga ctacatggta gcagaattca
    13381 aaaaagaaaa cagcattgac ttgtctcaag acaaaatggc tttacaacgc ttgaaagacg
    13441 ctgctgaaaa agcgaaaaaa gatttatctg gcgtatcaag cacacaaatc agcttgccat
    13501 ttatcacagc aggagaatct ggaccattgc atttggaaat gacattgaca cgtgcaaaat
    13561 ttgatgaatt aacagctgac ttagtagaac gtacaaaagt accagtacgt caagcgctaa
    13621 aagatgcagg tttatcacaa tcagaaatcg acgaagttat tttagtcggt ggatctactc
    13681 gtatccctgc agttgtagaa gcagtacgta aagaaacagg aaaagaacca aacaaatcag
    13741 ttaaccctga tgaagtagtt gctatgggtg ctgctatcca aggtggcgtg atcactggtg
    13801 atgtgaaaga tgttgtttta cttgacgtta caccattgtc attaggtatc gaaacaatgg
    13861 gtggagtgtt cactaaattg attgaccgca atacaacgat tccaacaagt aaatcacaag
    13921 tattctcaac tgcggcagat aatcaaccag cagtagacat ccatgtatta caaggtgaac
    13981 gcccaatggc tgctgacaac aaaactctag gaagattcca attgacagat attccagcag
    14041 ctcctcgtgg tattccacaa atcgaagtaa cattcgatat tgataaaaac gggattgtaa
    14101 atgtatctgc gaaagatcta ggtacacaaa aagaacaaaa aatcacgatc aaatcttctt
    14161 caggtctaac agacgaagaa atcgaacgta tggtaaaaga tgcagaagca aatgcagaag
    14221 cagataaagc tcgtaaagag gaagttgacc tacgtaatga tgtagatgca ttgctattct
    14281 ctgtggacaa aacattgaaa gaattagaag gcaaagtgga cgaagaagaa gtgaaaaaag
    14341 cagaaacagc tcgcgatgaa ttgaaagccg ctgttgaagc aaacaacatc gaagaaatga
    14401 aaactaaacg cgatgcattg aacgaaatcg ttcaaaactt gactgttaaa ctatacgaac
    14461 aagcggcaca acaacaagct caagaaaacc cagaagcagc tcaaggcgga gcagatgatg
    14521 tcgttgatgc tgattttgaa gaagtcgatg gcgacgataa ataatcagtt cactgaattt
    14581 cgagagaagg ccaaagttgc tactttggct ttttcttttg aaaaatagtt tgaagtatcg
    14641 gtttttgttt caaacaaaat atgatatgat aacaagcgac gtgaaaatga tgagaaccaa
    14701 taggaggaaa gcctatggca acaaaacgtg attattatga agtcttaggg ctctcaaaag
    14761 gagcatcaga agatgaaata aagaaagctt atcgtaagct ttcgaaaaaa taccatcctg
    14821 acatcaataa agaacctgat gcggaagaaa aattcaaaga agtatcagaa gcatatgaga
    14881 ttttaagtga cccgcaaaaa cgcgctgcat atgaccaata cgggcatgca ggcacagatc
    14941 caaattacgg tgctggtgga gctggtggcg gatttggtgg attcggcggc ttctctggtg
    15001 gcggcttcgg tggttttgaa gatatcttcg actctttctt tggaggaggc ggtcggtcag
    15061 tcgatccaaa tgcgcctcgt caaggtgctg atttgcaata tacggtcgac ttgtcttttg
    15121 aagaagcgat ttttggtgta gaaaaagaaa tcaagtataa tcgggaagaa atctgtcata
    15181 cttgtggtgg gaacggtgca aaaccaggca cacaaccaac tacttgtcat aaatgtcatg
    15241 gtgcaggtac gattaatgta gaacgccaaa caccacttgg acgagtgatg agccgtcaaa
    15301 cttgtgatgt ttgtcatggg acagggaaag aaatcaaaga accttgtcca acctgtcatg
    15361 gctcaggaca tgagaagaaa gcacattcag ttaaagtgaa tgtaccagct ggtgtagaag
    15421 aaggacaaca aatgcgttta gctggtcaag gagaagctgg agaaaacggc ggaccatttg
    15481 gtgatttgta tgtggtcttc cgtgtagaag aaagtgatat ctttgatcgt gaaggttctg
    15541 aaatttacta tgaattgcct ttaagttttg tacaagcagc tttgggggat gaagtccaag
    15601 taccaactgt ccatggagat gtgaaattga agatccctgc tggaacacag acaggtacga
    15661 acttccgttt acgtggaaaa ggcgctccga agttacgtgg tggtgcaaca ggcgaccaac
    15721 atgtcaaagt aaaactgatc acaccgaaaa atctaaatga acaacaaaga gaagcattac
    15781 gtgcattcgc tgaagctggc ggcatcaaag ttgaagaaca gcaagaagac ggtttcttcg
    15841 ataagtttaa agatgctttt ggcgggaaaa agcggaagta gtttttctag taaaaacgct
    15901 aagtcattaa taa
//
LOCUS       DAFEHS010000063        15803 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFEHS010000063
VERSION     DAFEHS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.065"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..1050
                     /locus_tag="LP327_002067"
     CDS             <1..1050
                     /locus_tag="LP327_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL1726170.1"
                     /translation="GTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYN
                     PEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGS
                     SDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEK
                     VDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEP
                     NAEVTVTLPDGSQYHGTADKNGNFTVKVPKLEAGTKVRVTATDESGNTSEPTNVVVSS
                     NKKDSGKNGSKGSKTDNQDGNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLG
                     TLGLLGYKNRKKENE"
     gene            1184..2527
                     /locus_tag="LP327_002068"
     CDS             1184..2527
                     /locus_tag="LP327_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL1726171.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKLILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GKEKIEKIVTTRDNKYAMDMVIQAIGFLSHSPIGKKEFLRDVSGAYLTDEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINDQSFTPSGSQFTNGFSIFG
                     YNFYAAGLNLKTARLLSEAIDYIEIEDYIRPIFSKTNNIVKLRISFEKKAKRIVGVQF
                     YSQEDLSGLLTMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNSGA"
     gene            2639..4042
                     /locus_tag="LP327_002069"
     CDS             2639..4042
                     /locus_tag="LP327_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL1726172.1"
                     /translation="MKKSLKLASAVLLMSHLLIATPFHVLASENSTYKIAKERTKTTE
                     MSNSDVTAEGSEFSTTSSSESSTTLNAESSTTSSSESSMTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTSQQIESTETFQSQPV
                     LSGNEEIHFDKNESVENFIMKIGDSARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGSFVIMVTNEDLGNGTLYKTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNGLIKTYDLTDYDKEVQGP
                     QMNKNGYQIPIHNYSISSLFGNRGDEFHRGLDMAAAQGEPIHAVKAGKVIKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVVQGQMIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGN"
     gene            4460..4675
                     /locus_tag="LP327_002070"
     CDS             4460..4675
                     /locus_tag="LP327_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726173.1"
                     /translation="MNQEIADIKLIKEVLQESTANAIASGSGISLSTAKKLKSGERNV
                     EKLNLSDAIKITEFAIKNRHVKIEIWK"
     gene            complement(4761..5672)
                     /locus_tag="LP327_002071"
     CDS             complement(4761..5672)
                     /locus_tag="LP327_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL1726174.1"
                     /translation="MKIKIERNQKEKSPKKNKERVISVGKHRKMVLALWLLLSCSLAF
                     GIYRNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWRNNKEAIEKRMSNL
                     EQYLTENGLALSQDMVRADIPTSSEVQSIKILDVEKHSEEFIVSFLVEQQITEGKKTQ
                     SISSAYRVTIFEDENGSHIVTSLPTMIAKPEKAKYESKQIGIESDIDAKTTEEVTDFL
                     ETFFKLYPSASEKELEYYVENKAVQPINSNLKLVELVNPTFKLKGKEIQGEIFVKYLN
                     NTTKVSNIFEFTLTLQKVKNWKIIDIG"
     gene            complement(5691..6695)
                     /locus_tag="LP327_002072"
     CDS             complement(5691..6695)
                     /locus_tag="LP327_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL1726175.1"
                     /translation="MNVKKLSIISLTILTLSFMVVLMCVGLLFGEDSEGSNSSGASSG
                     GSNVSEEVLKHRAMVEKYAKESNISEYVLILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVAKNGKKHNFALA
                     ESFSKEKSSGQKVDYPNPIAIKKNGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGASPETGFDCSGLTQWCYAKVGLKLPRVAQDQYNAMTHIDVKDA
                     KPGDLVFFHSTYDTGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            complement(6692..8815)
                     /locus_tag="LP327_002073"
     CDS             complement(6692..8815)
                     /locus_tag="LP327_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL1726176.1"
                     /translation="MKKKLLRIVGIIAISLCTILLLLSLIGTVAEATGLVDDTVKSGN
                     LYSQYSLDNYQLDFFVDSSWGWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQVSGFYFGFLLLMILALGVYVAYI
                     GLLKREATKAVRAVINFVTIFLLSGSFIAYAPTYITKINNFSSDVSEAALSLGTELVV
                     PNSESKGKDSVDLIRDSLFSIQVRQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRIMRKPHMLMNRKLR
                     QLNRNVIRTLAFGGGAAVGHKLEKEQSKSKFKPSGSSLRKNSRLPNDHEVPSDSTKEN
                     PVSNNKKQSRMNLAGRKMGKVLDTQALVKDKTKQVRDQVRNTPTNLKYNFHKGIEKTK
                     KAPKEFKRGLVQEKANRAELREKQRQHRDEKMAEKRKVLNETGNRHKKRRENVSIKPE
                     RQPQKDRAPKKEAPKQIVRVNPNPEIKRKLSSHEIVSKRISQPSSKKNSFQQVKQRST
                     LPKRTNQKVQKRMNPRPKSRSGEKK"
     gene            complement(8820..10100)
                     /locus_tag="LP327_002074"
     CDS             complement(8820..10100)
                     /locus_tag="LP327_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL1726177.1"
                     /translation="MHVRCGAGENLEITSKGYLSLFTSDFLAGLGFGATQMLGELEGI
                     YFGYNVDTGRNVYLKPALASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFG
                     GQAVIVDPKGERGSWKETLPEIASEINILNLTSKPENQGLLDPYVIMKKKKDSESLAI
                     DILTFLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVITELRNDGTPVAENIADHIE
                     SFTDYDFAHLLFSDGSITQSISLEKQLNIIQVADLVLPDAETTFEEYTTMELLSVSML
                     IVISTFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQ
                     NSDDLLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDESNQKRLRDLENGQCLM
                     QDLYGRVGVVQVHPVFEELFHAFDTRPPVQKRME"
     gene            complement(10140..11906)
                     /gene="ltrA"
                     /locus_tag="LP327_002075"
     CDS             complement(10140..11906)
                     /gene="ltrA"
                     /locus_tag="LP327_002075"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL1726178.1"
                     /translation="MQETFDWLYERSSKQATKGIDLYKIIMSEENILLAYRIIKSNTG
                     SKTSGIDNQTISDFKFQNKQKFVNDIRKTIENYQPQPVRRVNIPKSNGKTRPLGIPTM
                     KDRLIQQMFKQVLEPICEAKFFKHSYGFRPNRSTEHAIARCNFIAHKCKCHHVVDVDI
                     KGFFDNVHHGKLIKQLYTIGIKDRRVLAIISKMLKVTVAGQGTSTSGVPQGGVLSTLL
                     SNVVLNDLDWWIANQWETFQTKRKYSSVDNMHHALVKSKLKKMHIVRYCDDFKIFTTN
                     HKHAVKIYHAVKGYIKQRLNLDISPDKSKITNLRRNYSEFLGFELKVIKQKKQQYVTV
                     SRVSKKSKSRIKKEIREKIKAIQKSPTRKTVNQYNQYVRGIQNYYQKATLINHDFSSI
                     YYSCLPALYNRLKPFGKYEIPRSPPITYKKFYPLTKRTFCVRKNYLFPLDSVKWKRLE
                     FFKPHINNYTQDGRLAKLRKLKPSVYSELVTLSTMETDESLEYLDNRLSRYSMQNGKC
                     SVTGIFLRTQDVHCHHIIPRKFGGTDEFDNLVIIHKWVHRLIHAKTKETIKEYLQLLQ
                     LTNKQMKKLNKYRLECNLTEFH"
     gene            complement(12650..13879)
                     /locus_tag="LP327_002076"
                     /pseudo
     CDS             complement(12650..13879)
                     /locus_tag="LP327_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            complement(13866..14255)
                     /locus_tag="LP327_002077"
     CDS             complement(13866..14255)
                     /locus_tag="LP327_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL1726179.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHMPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14338..15063)
                     /locus_tag="LP327_002078"
     CDS             complement(14338..15063)
                     /locus_tag="LP327_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726180.1"
                     /translation="MLEVMIIMNFDFDLKSFIDIFVPVLTSFAVCFITLFFTNKQHKK
                     AMKQQERQHEESLDLSKQQYADEKQRNEEQDRLSYYPYLKLIPKISEHRFAGKMVKSD
                     ENDVFKLPFELINEGLGMAFSIDFVYLEETINTNDHEVNLLSLLSAQKHIDDSLDALG
                     VSAPIDTDILRVGKKTEFELYWMKYNNEVMSIPKYSVSKWEIKIRFSDFQDRIYQQRY
                     TFITSAGYQEIHRLWSDKPQLID"
     gene            complement(15112..15615)
                     /locus_tag="LP327_002079"
     CDS             complement(15112..15615)
                     /locus_tag="LP327_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL1726181.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFSPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPIGEVALEIQHAFFNSFEEMVEH
                     VDDIICYPDCNDMSDVAYYLIEETGAFGEVPTHLQNYIDYEAYGRDLELEGNYLVTNR
                     GTFEFIG"
     gene            complement(15627..>15803)
                     /locus_tag="LP327_002080"
     CDS             complement(15627..>15803)
                     /locus_tag="LP327_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726182.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
BASE COUNT         5053 a         2962 c         2686 g         5102 t
ORIGIN      
        1 ggtacaattg ataccaacac accaggcaat tacgaaggtg tcattgaagt aacgtatcca
       61 gacggtacga aagatacagt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      121 gataaataca atcctgaagg acaaaaagtg acgacagact taaataaaga gccagatgca
      181 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      241 aaagtagatg ttagcacagc tggaaacaaa aaaggtacta ttgtagtgac atatccagat
      301 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      361 aaatacaatc ctgaaggaca aaaagtgacg acagacttaa ataaagagcc agatgcatct
      421 gagggaatta agaacaaaga agatttacca aaaggaacta cttatacttg gaaagaaaaa
      481 gtagatgtta gtacagctgg aaacaaaaaa ggcactgttg tagtaacata tccagatgga
      541 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      601 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      661 gaggtaacag ttacattacc agacggaagt caataccatg ggacagctga taagaatggt
      721 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgagagt aactgcaacg
      781 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
      841 agtggaaaaa acggtagtaa aggtagtaag acggacaatc aagatggcaa tagcaatcaa
      901 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
      961 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1021 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1081 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1141 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1201 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1261 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1321 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1381 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1441 aaataaaaag attagttgtg taaaaaaaga tggcacaaga ttttcccaag aatatgacaa
     1501 acttatatta gctacaggtt caaagcaaat tatgttagat ataccgggga ataatttagc
     1561 taatattttt tcgataaaga attttccaaa tgccaaagag attttcaaag ctttagatga
     1621 taatactatt aaaaaagtag gtattattgg agcaggatat attggagttg aaattgctga
     1681 agctataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     1741 gacctattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaacgggat
     1801 acatctttgt ctatcagaaa gtcctaaaaa gtatttagga aaagaaaaga ttgaaaaaat
     1861 agtgactaca agagataaca aatacgctat ggatatggtc atacaagcta taggattcct
     1921 atctcatagt ccaataggaa aaaaggagtt cttgagagat gttagtggag catatctaac
     1981 ggatgaatac caacaaacaa atataaaaga catatatgct attggagatt gtgctacaac
     2041 tttttttaca tccattaata gaagtagcgt tgatttttct atctctaatg ctctaagaac
     2101 ggcttatatt gcaactcaac atattaacga tcaaagtttt acaccttcgg ggtctcaatt
     2161 cacaaatggc ttttctatat ttggttacaa tttttatgca gcaggtttga atttaaaaac
     2221 agcacgctta ttaagcgagg ctatagatta tatagaaata gaagattata ttagaccgat
     2281 tttttctaaa acaaacaata ttgtaaaatt aagaattagt tttgaaaaaa aagcaaagag
     2341 aatagtaggt gtacaatttt atagtcaaga agatctatct ggattattga caatgttctc
     2401 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2461 tcctagtttt agtcaaccct ataattttat tttaaaggca gtaacaggat taaatagtgg
     2521 agcataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2581 atttgtcata ttttttaata gaaaacacta ttaaagaaaa aaaagagagg taaatcaaat
     2641 gaaaaaatct ttaaaacttg catcagcagt tctattaatg tcccatctcc ttatagcaac
     2701 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccaa
     2761 gacgactgaa atgagtaatt ccgatgtaac agcagagggt agtgaatttt caacaacttc
     2821 atcaagtgaa agtagtacga ctttaaatgc tgagtcttca acaacttcat caagtgaaag
     2881 tagtatgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgatttc
     2941 aagtaccgaa tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagttac
     3001 aaagccaaac catatagagt cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa
     3061 aactttttcg acatcacagc aaatagaatc aacagaaact ttccaatcac aaccagtact
     3121 atctggtaat gaagaaattc attttgacaa aaatgagtct gtagaaaatt ttattatgaa
     3181 aattggtgat tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat
     3241 tgcacaagca atcttagaat ctgccagtgg tcaaagccaa ttagctcagg caccgaatta
     3301 caatttattt gggataaaag gaacttataa tggaagcttt gtcataatgg taacaaacga
     3361 agatttgggt aatggaacgc tttataagac tcaatcgaaa tttagagtgt atgaaaacta
     3421 tgaggaaagt tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga
     3481 tttttatgcc ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct
     3541 tacaggacgg tatgcaacag atactcaata ctatttaaag ctaaatggat taattaaaac
     3601 ctatgattta actgactatg acaaagaagt tcaaggacca caaatgaata agaacggata
     3661 tcaaatacct attcataatt attcaatatc tagtttgttt ggaaatcgtg gagatgagtt
     3721 ccatcgagga cttgatatgg cagctgcaca aggtgaaccg attcatgcag ttaaagctgg
     3781 taaagttatt aaagcagagt ttcactattc ttggggaaat tacgttgtta tacaacacga
     3841 agatggatca acagcgttat atgctcacca acaacaatac ttagtaaaaa ctggagacaa
     3901 cgttgtgcaa ggacaaatga ttggctatgt aggaagtact gggaatagta caggaccaca
     3961 cctccatttg gaaatttgca aagatagtag tctttctcaa agcatgttag ttgatccgaa
     4021 aaccttatta tttgggaact aaaaagaaaa agagcatttt attagaagta tggattaggg
     4081 aatatagtag tagtgggctc taaagattat gaaatcttta agagctcttt ttaagacgat
     4141 tgtttaaaac gaattaacca agtaggagct ttcctagaat ttataaaatt atagtttctt
     4201 gtatgacatg tatatagcat gctaaaatac caataaataa ggtttaagat aaacgcaaaa
     4261 agtcgaaaaa ttaacactta ttttgtgata ttaattttaa tttgcaaaac aagctatatg
     4321 gaaaaagttt aaactttttc tagagtccct attactagtt aaaaagacca ctgtagtcct
     4381 ctgataaatc atcataaaga ccacagtagt ccccttaaag tttttccaaa ttaactatct
     4441 gagaaaggag agaagatttt tgaatcaaga aatagctgat attaaattaa tcaaagaagt
     4501 tttacaagaa tctaccgcaa acgcgatagc atctggatca gggattagct taagtacagc
     4561 aaaaaaacta aaaagtggcg agagaaatgt agaaaaacta aatctttctg atgcaataaa
     4621 aatcactgag tttgctatta agaataggca tgtgaaaatt gaaatttgga aatgacggat
     4681 acaactatta tagttttgtt ttttctaagt atcatattta tgaatatgct tttgctaaac
     4741 atctaattat agattaattt ttatccaata tctataattt tccaattttt taccttttgg
     4801 agagtaaggg taaattcaaa gatattgctt actttagttg tattattaag atacttaaca
     4861 aaaatttctc cttgaatttc ttttcctttc agtttaaaag taggattaac caattcaact
     4921 aatttcaaat tactattaat tggctgaact gctttgtttt caacatagta ttcaagttcc
     4981 ttttccgatg ctgatggata cagtttaaaa aaggtctcta aaaaatcggt gacttcttcc
     5041 gtggttttag catcaatgtc agattcgatt cctatttgtt ttgattcata ttttgccttt
     5101 tctggctttg caatcatagt aggcaaactc gttactatat ggctcccatt ttcatcttca
     5161 aagattgtca ctcgatatgc agaagaaatt gattgtgtct tttttccctc tgtaatctgt
     5221 tgctcgacta aaaatgaaac gataaattct tccgagtgtt tttcaacatc tagaattttt
     5281 atagattgaa cttcggaact cgtgggaata tctgctcgaa ccatatcttg gcttaaagcg
     5341 agaccgtttt ctgtaaggta ctgttcgaga ttactcattc tcttttcaat tgcttccttg
     5401 ttatttctcc atgaaaaata ttctttcgca aaatcctctg taaaactttc aacgcctgaa
     5461 gtattcacta ccttctcttt tataactact ttttcatgag tcgtatgctg atcaatggct
     5521 gtgaaatttc tgtagattcc aaaagcaagg ctacaagaca ataaaagcca aagagcaagc
     5581 accatttttc gatgcttgcc cactgaaatg actcgttctt tattcttttt tgggctcttt
     5641 tccttttgat tccgttcaat ttttatcttc atgttttttc ctcttttctc ttatttgtac
     5701 cgtccagcac cgataagatg ctgctgccaa tagctttctg ttagatttgt ccagcccact
     5761 ggatctcctg catgaaacat gcgattctct cctgcataaa taccgacatg ggtcacataa
     5821 gtaccggtat cataagtcga atgaaagaaa accaaatcac ctggtttggc atcttttaca
     5881 tcaatatggg tcatggcatt atactgatct tgagccaccc gaggaagttt cagaccaact
     5941 ttggcataac accattgagt caagccacta caatcaaacc cagtttcggg acttgctcca
     6001 ccaaacacat aagccgttcc ttcatactta aacgcctcat caaaaatccc ttggactgtt
     6061 ttatcatcaa actgtgttgt agttaggtat tgcttcacta ataagcaata aaacatattg
     6121 ccatagttat accgccaccc accatttttc ttaatcgcta tgggattagg gtaatcaact
     6181 ttttggccgc tcgatttttc ctttgaaaaa gattcggcta aagcaaaatt atgcttcttc
     6241 ccgtttttcg cgacatgatc cagatacccg ccaccatagt tatacgcttg aataatgcta
     6301 tcttgatcac accctagttc tttggaacgc ttaaccaaag acgcaaaata gagacatcct
     6361 tgtttgatgg atgcctcggt acttaatgaa ttcggtggca acccggctga ctccgaactc
     6421 tgcatcacat cttccattgt gccaccagat tccacttgaa taatcgccaa aagaatcagg
     6481 acatattctg agatgtttga ttccttggca tatttttcca ccattgctcg gtgtttcaaa
     6541 acttcttctg agacattact tcccccagaa gaagcacctg aactattact tccttcactg
     6601 tcttccccaa acaacaagcc cacacacatc aagacgacca taaaggagag tgtcaagatg
     6661 gttaacgaaa tgatacttaa tttctttaca ttcatttttt ctcacccgac cttgattttg
     6721 gacgagggtt cattcttttt tgtacttttt gattggttcg cttgggcaga gtagacctct
     6781 gtttcacttg ttggaaagaa tttttcttac ttgatggttg acttattctt tttgaaacaa
     6841 tttcatgact agatagtttt cgtttgattt cgggattcgg atttactctc acaatctgtt
     6901 ttggtgcttc tttttttggc gctctatctt tttgaggttg gcgctctggt tttatcgaaa
     6961 cattttccct tcttttctta tgacgattac cggtttcgtt caatactttg cgcttttcag
     7021 ccattttttc atctctatgt tgtcgttgtt tttctctcaa ctccgctcga tttgcttttt
     7081 cttggacaag tccgcgcttg aattctttcg gtgctttttt cgtcttttca attcctttgt
     7141 ggaagttgta tttcaagttg gtcggcgtat tccttacctg atcccttact tgtttagtct
     7201 tatctttcac caaagcttgg gtatccagaa ctttgcccat ttttctccca gccaaattca
     7261 ttcgggattg tttcttatta tttgaaactg gattctcctt agtcgagtca gatggtacct
     7321 cgtgatcgtt aggtaatcgt gaattctttc gtagagaaga accggatggc ttaaatttgg
     7381 attttgattg ttccttttcc aacttatgtc caactgctgc accaccgcca aaagccagag
     7441 ttcggatcac atttcgattg agttgccgca atttacggtt catcaacatg tgcggtttcc
     7501 gcatgatgcg ccttcccatt gactgactat cattgctttg gagattgaac atactcatga
     7561 tgtcacctaa tttgaaatag ataccggcaa aggtcacgat ttgcaggaac gcaaccataa
     7621 agaaaggata ggtagctgaa atattgaaaa acatggtgga gatactaaaa gcagtcgtaa
     7681 tgactaaggt aactcccgct cgcatcataa tcgtattgaa taaacgaata atcgccttct
     7741 ttcccaagct ctcataagtt ggtaacatgg ataacagaaa actaattggc agaaacatgg
     7801 cgaaaatgat aaagagaatt tgggagaaca acataatccc cgtcagtaag aagacaaaga
     7861 atgagatacc aatattaaac aacaccaaaa agaccacggt ccctagtcgg ttcatggtct
     7921 ttgtaatact tagattcgcg ttgtcattgt cttcaatttc agcttttacc gcttcttctc
     7981 gatcctttcc cttattttca tctggactca cagataaaat tttattgacc cggtcttcac
     8041 caatttcttc tatgttggaa tcatcaaatt gcaacaagag ccaaggttgt cggacttgaa
     8101 tagaaaacaa actatcacga atcaaatcca cactgtcttt tcctttgctt tccgaattcg
     8161 gcacaacgag ctctgttccg agactaagag ccgcttcact aacatccgaa ctaaagttat
     8221 tgatttttgt gatataggta ggcgcatacg cgataaagga accagagaga aggaagatcg
     8281 ttacaaagtt aatgacagcc cggactgctt tggtagcttc tcgctttaat agtccgatat
     8341 acgccacata gactcctaga gctaaaatca ttaataatag aaatccaaaa tagaaacccg
     8401 agacctgtaa tccattttca gtaataccgg ctagcgtttg gatatttttc ccaatacttt
     8461 ctgccgtatc cgaaataaaa tctaatttgt acgcttcttg taccacatac ccggtcgcat
     8521 ttgataaata caaactaacg gtccaaatga aatttgtaat ggcgtagaga ccgtacatca
     8581 cactctttcc taacccatcg ccccagttcc aaggcagcca gccccacgaa ctatctacaa
     8641 agaaatccaa ttgatagtta tcaagtgaat attgagaata gagattgcca gatttaacgg
     8701 tatcatcaac taatccagtt gcttccgcta ctgttccaat cagactcaaa agaagtaaaa
     8761 tggtgcagag actaattgca attattccca caatccgtaa aagtttcttt ttcatctcct
     8821 cactccattc tcttttgaac aggcgggcgt gtatcaaaag catggaagag ttcttcaaag
     8881 actggatgca cttgtaccac accaacgcga ccatacaagt cctgcatcaa acattgccca
     8941 ttttccaaat ctcgcagacg cttttgattg ctttcatctt cttgatcaag accaaagaat
     9001 tcgagtgtct tcttgatttc atgaatatct gtgcttcgaa aggcaaattt cagcccaatg
     9061 ttgtttttca gacgttcatc taataaatca tcactatttt gagtgacaaa gtaaacgccg
     9121 gcattcatag cacggccagc acgaactagt ttattcgaaa gagtttttcc ttgtgcgact
     9181 tgaaggaagc tccaggcttc atccaaatcc acgattttga aaatttctcg attgctatga
     9241 ataaaatcca aggcaaatgt ggagataaca atcaacatgg aaacactaag caattccatc
     9301 gtagtgtact cttcaaatgt agtctccgca tccggcaaaa ccaaatctgc cacctgaatg
     9361 atattcaatt gtttctctaa actaatcgat tgtgtaattg aaccatctga aaaaagtaaa
     9421 tgcgcaaaat cataatcggt aaacgattca atatgatcag caatattttc cgcaactggt
     9481 gtcccatcat ttcgaagctc ggtaatgact tgtaacaacc cacgttgatt gctttgggta
     9541 acggttcgaa tagcttttcg taaaacggga aatttctctc catcccgact agaaattcca
     9601 gttagaaaag tcagaatatc aattgctaga ctttcagaat ccttcttttt cttcatgatc
     9661 acatagggat caagtaaccc ttgattttct ggtttactgg ttaggttgag gatattaatt
     9721 tcactcgcaa tttctggtaa ggtttctttc cagctaccac gctctccttt gggatcaaca
     9781 atcaccgctt gaccgccaaa caggacagca tagtaaacta aaagattatt actaaaggat
     9841 ttcccaccac caagtgaacc aagaaaagcg gcggccagtg cattggtgac ggaacctttg
     9901 accccttgtg aagccaatgc cggtttaaga taaacattgc gtccagtatc cacattgtag
     9961 ccaaaataaa ttccctccaa ttctcctaac atttgggttg ccccaaagcc aagcccagca
    10021 agaaaatcag aagtaaatag cgataggtaa ccctttgaag ttatctctag gttttccccc
    10081 gctccacacc gtacgtgcaa ctttcatcgc atacggcgtt ccaacttgtt cgtatttctt
    10141 tagtgaaatt cagttaaatt acattctaaa cgatatttgt ttaacttctt catttgctta
    10201 tttgttagtt gaagaagttg tagatactct ttaatcgttt cttttgtttt ggcatggatg
    10261 agtctgtgta cccatttatg gataataact aggttatcaa actcatctgt acccccaaat
    10321 ttacgaggaa taatatgatg acaatgaaca tcttgcgttc ttagaaaaat ccctgtaacg
    10381 gaacattttc cattttgcat agaatatctt gacagtcgat tgtctaagta ttcaagactt
    10441 tcatctgttt ccatcgtact aagggtaacc aattcagaat aaaccgaagg ttttaatttc
    10501 ctcagtttag ccaatcgtcc atcttgcgta tagttgttga tgtgaggttt gaaaaattct
    10561 aaccttttcc atttcacgct atctaatgga aataaataat tcttcctaac acaaaacgtt
    10621 ctttttgtga gaggataaaa ctttttatac gtaatgggtg ggcttctagg gatttcatat
    10681 tttccgaatg gttttaaacg attatatagt gctggtaagc aggaatagta gatagaagaa
    10741 aagtcatggt taatcaaggt ggctttttga taataattct gaatacctcg tacatactgg
    10801 ttgtattggt taacagtttt tctagtagga gatttctgaa ttgcttttat cttttctctg
    10861 atttccttct taattctact ttttgatttc tttgagactc tcgaaacagt aacatattgt
    10921 tgtttctttt gttttatgac ctttagctcg aatcctagaa attctgaata atttcttcta
    10981 agatttgtaa tttttgattt gtctggtgaa atatctaggt tcaaccgttg tttaatatat
    11041 cccttgaccg catgatagat ttttactgca tgtttatgat ttgttgtaaa gattttaaaa
    11101 tcatcacaat aacgtacgat gtgcattttc tttaatttcg actttaccaa agcatggtgc
    11161 atattatcta cactgctata ttttcttttt gtttgaaatg tttcccattg attggcaatc
    11221 caccaatcta agtcatttag aacaacattc gataataatg tagataacac gccaccttgt
    11281 gggacgccag aagtggaagt gccttgtcct gctactgtca cttttaacat tttagagatt
    11341 attgctaata cgcgtcgatc ttttataccg atggtataaa gctgtttgat taactttcca
    11401 tgatggacgt tatcaaagaa tcccttaatg tcgacatcta ccacatgatg acatttgcat
    11461 ttatgtgcaa taaaattaca tcttgctatt gcatgttctg tagagcgatt aggtcggaac
    11521 ccataggaat gtttgaaaaa ttttgcttca catatgggtt ctaacacttg tttaaacatt
    11581 tgttgtatca agcggtcttt catagtggga atgcctagag gacgagtttt cccattactt
    11641 tttggaatgt tgactcgtct aactggctgc ggctgataat tttcaattgt ttttcgaata
    11701 tcatttacaa acttttgttt gttttgaaat ttgaaatcac tgattgtttg attatctatc
    11761 ccagatgttt tagaaccagt atttgattta ataattctat atgctagtag gatattttcc
    11821 tctgacataa ttattttata aaggtcaata ccttttgttg cttgtttgct acttctttca
    11881 tagagccaat caaaagtttc ttgcatttga tagtattcat aatatcgtaa ttctgtactc
    11941 actgatggat tatttctcct aactttaaaa ttagtcccat cctcttacca gatcttgtga
    12001 gtttcattta actgtattca cttttcaaag aacaagtctt ggggctatcc ctccacgttg
    12061 attagacgct tcacaggtac tgtgccccta ctctcgcaga aattaaggat tttcgactat
    12121 tagtcttata tccaacgctc caataacagt tgttgaataa gtgttttctg ttttcctcgt
    12181 tccagttatt ctagccatac atctatcctt aggtgcttac tttgagcctg aatattatat
    12241 aaggtcattg ttccttatcc cgaatttcat aaatacgact cttacttttc ggtaatattc
    12301 gttgcttttg caacccaatt atctttcgat aaaagtcatc ctttagaccc gtacattcgc
    12361 aagttcgtca gttccgtagg aacattctca ccatagatag tttgtggcgt cccggcatat
    12421 cctttaccat ataagttatt acttacttag gtattgttct gccgacttta cctagcttca
    12481 cacgaatatt cactgttatg aatattcgca tgtaggagta tcagaccagt tgcttcaacc
    12541 aacttgacgg ttttcatttc aactgtgaaa taactagttg taattatctt tagttcttga
    12601 taattctccc atttcatgcc tatgcactta tagggaaacg agtcgcacca tattggatat
    12661 agtcattcat gtatcgcttg ctagctggca aaaactcttc atgaagtccc atcatatcgc
    12721 caaagggacg aaccaatttt acattggtat catcataaaa atctaaaacc tcatcacacc
    12781 gacgttttaa ttcatccaat gattctgcac tcactcgaac aacatagctt aatttgtaca
    12841 tggattcttt ggattgatcc aacgttgttt ctagttcatc cacggagtcc aacgcatcca
    12901 agacattgga attggtttca ttgtcggact ggtacgcatg gttgtctaag tctttcagtt
    12961 cttttttctt attgcgaact gtcgccagtg cctttttatt cgtcacgatt tctacattca
    13021 tactcgtgtc gattgggaaa gtgaattgtt gttgctggta ataaaagagt tcagaagatg
    13081 gaaactccat ctcaccgaca atcgtattaa tcgttagata ggccacataa gattcatgat
    13141 tttcatgttc catgtgtaag taccgaggct tctcttcaat caaacagcga cttgggcgaa
    13201 gtaggtcata gcgctttatc aatgtttcag attttagctt tttcttagac aactgaaatt
    13261 catattcttc aaatggtgtt cctttttctc cataaatgtg ttcaatcaaa taagttagat
    13321 cactaggtgt tactcgtctt actttgaatc tacgagccaa tttactttcc atcaattttt
    13381 caagcttagt ataccggcga atttcttcat tggataacga aacaaaatca cccatcaaat
    13441 gatgattcac tccatagaca aactcttgaa gtccggataa aaaagagttc ttgagacttt
    13501 tcaggtttac ttcttcgtct gtgacaatca gtttgaagcc aataaaaaag cgatagtcaa
    13561 tttgactatc gccaatcatc gataccaatg catccgtctg taagtcgatt ctctgttttg
    13621 cgacttcttt caaacgacca gttatctctt ttttcgaccg ttcctgcgtg gcacgaatgc
    13681 tgctttctgt cgcaatttgt aacgcatgaa tcttaccttc acgattttgc gcaatcaatt
    13741 ggcgaaagtt atcgtggact tgatacttct gttcaagtga taagaaggag taattgtaag
    13801 ggactagttc gtagtaagca aagcactctc cctcttggtt aaaaacaaga ttattttcaa
    13861 tatatttaat cgggtacata attcacactc ctcactaggg tgattggttc atcccatcgt
    13921 tttttctgtt ctttgacttt ctttcctgca aaagtaacct taaatcgcaa gtgataacta
    13981 ataaaggatc gtaaaaaccc taatggcttc tttccatcaa aggtcttttg cgacataaac
    14041 caagtgactg ccacaggtat gcccaagtat tttaaaaatg ccccctctat catagatagt
    14101 ggaggcatat gagcaaacac aatgacaaac agcaaggata agacaaacca agccatttga
    14161 ttaaaggtta caggaaaagg cagctgaaag tcattgattg cgtaaatgac tttttcaact
    14221 gcccagatac tggtatagct ttttattttt ttcattagtt tcacttccta aaaaagcgcc
    14281 tgataaagac gactctgcat gaatattaat tttcttttta aaagtgtatt ccaactttca
    14341 atctatcaac tgaggtttat cagaccataa tctgtggatt tcttggtatc cagctgaagt
    14401 aataaaagta tatctttgtt gatatattct atcttgaaaa tcactaaacc gtatttttat
    14461 ttcccattta ctcactgaat atttagggat actcattact tcgttattat atttcatcca
    14521 atataattca aattctgttt ttttccctac tcttagaata tctgtatcaa taggcgctga
    14581 aactcctaac gcatccagac tatcatcaat atgtttttga gccgaaagta atgataatag
    14641 atttacttca tggtcgtttg tgttgatagt ttcttctaaa tacacaaagt caattgaaaa
    14701 agccattccc agaccttcat taataagctc aaatggtaat ttaaaaacat cattttcgtc
    14761 agactttacc attttaccag caaaacggtg ttcagatatt ttgggtataa gttttaaata
    14821 agggtaataa cttagacggt cctgctcttc atttctttgt ttttcatcag catattgttg
    14881 ctttgataaa tcaagtgatt cctcatgttg tctctcttgc tgtttcatcg cttttttatg
    14941 ttgtttattt gtaaaaaaaa gtgtgataaa acatactgca aatgaagtta gtacagggac
    15001 aaatatgtct ataaatgatt taaggtcaaa gtcaaaattc ataataatca taacctccag
    15061 cattatatat ttatcaataa ttatatccct aaacctatat catgaaaatg tttatcctat
    15121 gaattcaaat gttcctctat ttgtcacaag ataatttcct tctagttcca agtcacgtcc
    15181 atatgcttca tagtcaatat agttttgcaa gtgtgtaggc acctctccaa aagcacctgt
    15241 ttcttcaatc aaataatacg ccacatcact catatcgtta caatcgggat aacagataat
    15301 atcatccaca tgctccacca tttcctcaaa agagttgaag aaagcatgtt ggatttccaa
    15361 agcaacttca ccaatcggtg ttccttctag ttcctctgct aaaccacaca accgattgat
    15421 ttcctcaatt ggtgtatatt catcaatctc aaaaggcaac tcataatcat ggatcgcata
    15481 ctcttcgtat tcgtcattta aaccaatccg ttctttcact tcgtcaaaat cgacaggtgg
    15541 gctaaaccaa gcccccacca attcaccttc gttatatttt ccaaggttcg caatatatac
    15601 acgcatctgc tccataatgg tcccccttac gcaccgacaa tccgattaaa caagttgagt
    15661 aagacatctt tcacaccaga agcgttgaag accagtccta ccgcaatcag cgcaatcacc
    15721 aaaaaaccaa tcagcttgga aaactcacgt ttgaagccta aaaataaacc aatgaccaca
    15781 attgccaata agactaaaga ttg
//
LOCUS       DAFEHS010000064        15456 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFEHS010000064
VERSION     DAFEHS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.066"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(148..450)
                     /locus_tag="LP327_002081"
     CDS             complement(148..450)
                     /locus_tag="LP327_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726183.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(670..1036)
                     /gene="ssrA"
                     /locus_tag="LP327_002082"
     tmRNA           complement(670..1036)
                     /gene="ssrA"
                     /locus_tag="LP327_002082"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1207..1980)
                     /locus_tag="LP327_002083"
     CDS             complement(1207..1980)
                     /locus_tag="LP327_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL1726184.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2124..2474
                     /locus_tag="LP327_002084"
     CDS             2124..2474
                     /locus_tag="LP327_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL1726185.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2455..3171
                     /locus_tag="LP327_002085"
     CDS             2455..3171
                     /locus_tag="LP327_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1726186.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3171..4619
                     /locus_tag="LP327_002086"
     CDS             3171..4619
                     /locus_tag="LP327_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL1726187.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4690..5199)
                     /locus_tag="LP327_002087"
     CDS             complement(4690..5199)
                     /locus_tag="LP327_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726188.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5189..5914)
                     /locus_tag="LP327_002088"
     CDS             complement(5189..5914)
                     /locus_tag="LP327_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726189.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6137..8257)
                     /locus_tag="LP327_002089"
     CDS             complement(6137..8257)
                     /locus_tag="LP327_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL1726190.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8487..9665
                     /locus_tag="LP327_002090"
     CDS             8487..9665
                     /locus_tag="LP327_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBL1726191.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9681..10439
                     /gene="aroD"
                     /locus_tag="LP327_002091"
     CDS             9681..10439
                     /gene="aroD"
                     /locus_tag="LP327_002091"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBL1726192.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10462..10947
                     /gene="ybaK"
                     /locus_tag="LP327_002092"
     CDS             10462..10947
                     /gene="ybaK"
                     /locus_tag="LP327_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBL1726193.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11018..12271)
                     /locus_tag="LP327_002093"
     CDS             complement(11018..12271)
                     /locus_tag="LP327_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL1726194.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12388..13737
                     /gene="gdhA"
                     /locus_tag="LP327_002094"
     CDS             12388..13737
                     /gene="gdhA"
                     /locus_tag="LP327_002094"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBL1726195.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13849..15195
                     /locus_tag="LP327_002095"
     CDS             13849..15195
                     /locus_tag="LP327_002095"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBL1726196.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         5082 a         2743 c         3121 g         4510 t
ORIGIN      
        1 agtgacttat ttttgatttt atagcactga tactataaaa tatagatatc atcatattac
       61 acaatcttaa tactaactta aaaaatatcc cttttataag tatggtgata aaatccgttc
      121 cgggctacct tttttaggta gcctacttta atctttatac ctttctggat caacgaaagt
      181 atacttcaaa tagtcataac gccgatgatc gctccgtgcg tctggcacat cagtcacgat
      241 atcaaacaaa aaatacacat ctttcttcat tctggttttt gttgccggaa ttttaaagta
      301 gttcttatta gagtaataga gattgattaa taagctatct tcgattgcta aaaagaaaac
      361 ttctgaatcc cacaccttat aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa
      421 ccataattgt gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaact
      481 aatgttcttt ttgtaaagta ggaatatcat tgataatatg gatggaacat atatattaaa
      541 aagtgttctt tttgaatgct attttgaatg ccattttcat tcatttaacg aaaatctgcg
      601 aaaataaaaa atgctcaaac ccttataaaa taaggattta agcatctagc gaaatctgct
      661 gaaatctcac aatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa
      721 aatctacgtt catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc
      781 attctgtatc gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta
      841 tcccactaaa gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta
      901 actgttttta ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact
      961 gtaacgtttt tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg
     1021 aatccgtaac gtccccgtta gctaacaagg gttacgtgta tttttcatac aaaattagta
     1081 tagcatatat tgggataaat tcaataaaaa agcgaatcat atcccattat ttatataata
     1141 actgcccctt tcttaagaaa aaataagcga ttttcttaag aaaagaggta gcttcaaaaa
     1201 aagatcttaa atcagatcaa aatacttcaa tgcgtggaca attccgcctt ctgtattttt
     1261 ctttgtgata aaggtagaaa gttcttttaa tccgtcatag gcgttgccca tagcgatttt
     1321 gttgtcgcaa gcttccaaca aggcaaaatc attcggacca tcgccaaatc caaaagtagg
     1381 aactttttcc agctgaagtt ctttttgcag acgtttgaca cctgcccctt ttgagattcc
     1441 tttcttcact gtatcgatcg agtaaggacc attccgataa aaagttagct caggaaaatg
     1501 ttcgtgatag tattcgtcac ctgattgaga taagatcagc aacatattta ctgtgttttc
     1561 tttaaagcct aaaggatcaa tttccggaac atctgaatga atgaagtcgt aagcattgat
     1621 cattgtttgg ctatgaccgg tacaccagat tttctgatcg ttatagtaag aaagttcatg
     1681 accgttcaac tgtacatgat catacatttt ttgacattct tctgaagaaa atgtttgaga
     1741 atagatttct tcgccgtcta ctcgaatgaa agaaccattc atggtgaccg cagaagtaat
     1801 gcccgcatct tccatgatag agtggatttc tgcttctgta cgtcctgtag cgatgacggg
     1861 gagaacattg ttttctttta atgcgttcat agcttttgcc acttcgggtg tgatttttga
     1921 tttcgcatct agtaatgttc catcaagatc aaagaaagtg attgcacgat aatttgacac
     1981 aaataaaact ccttttttat tggaataagt ttatcttaac aaagaatgaa caaaagacaa
     2041 acctttcgtg aagaattcct gaacatattg gttcagtcaa caatttagag tatactataa
     2101 gaagagaatt taggagggaa agaatgattt ttttcagtat tggtatctta ttcttaataa
     2161 ttggcagtct ttttcggatt ctgccctcaa aaggaaatct gcctttttat gggtatcatt
     2221 cgcctcttgc cgcaaaaaca gacgctcatt ggcggctggc tcaaaagaca agcggaaact
     2281 ggttttttct tatgggctta ctgatggcat tgatcggcta ttatttaaag acatctgggc
     2341 atacgaatta ttttctgatt gaaatgcttc tgctagtatt tccaattatg cctattttta
     2401 ttatgacaga aaagaaatta cagaaatatg acctggaaac tggagggaac gataatgaat
     2461 attttaatga ttgaagataa tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat
     2521 gaaggttggg aagcaacttt caagtatgat ggaaaagaag ggctcgatgc ctttttagaa
     2581 aacccacaac attgggatat gattacacta gacctgaatt tgcccacact ggacggtatg
     2641 ctagtaggaa gagagatccg caaagtttca agtactgtgc cgattatcat gctgacagct
     2701 agagattctg aaagcgatca ggtgatcggc ttagagatgg gggcagatga ttatgtcaca
     2761 aaacctttca gtcctctgac gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag
     2821 ttggcagaag caagagaaga agcagtagtt tcagatgatc attttgatat cgagactagt
     2881 catttcaaga tgaatacgaa aacacgagaa gcgtatctgg acaaccggcc aattgaagga
     2941 ctgacgccta aagagttcga tctattgttt actttagcaa aaaaaccacg tcaagtattc
     3001 tctcgggagc agttgcttga attagtctgg gattatcagt actttggtga tgaacggaca
     3061 gtggacgccc atatcaagaa attacgacaa aaaatcgaaa aggtcggacc acaagtcatc
     3121 caaactgttt ggggagtcgg ctacaaattc gatgattctg gtgttgccta atgcgttatt
     3181 tgtatcagca actcttagct ttctgcggaa tggtcgcgtt gatcttattg atcgttggag
     3241 tttcttttac acaactgaca aaacaaacga ttgaagaaaa caattaccag caactgtttg
     3301 gatatgctga atcagttgaa aagacaacgc aaacctatgc cgatacgctc ccgcagttta
     3361 gtcaagatca ggcgtttcaa aatgcattgc tgctgacaga gcaagtactg acagaacaaa
     3421 atgtgaactt tatatttatc gataaaaacg aacgtgtcat ttatccgact aatacagctt
     3481 tactgaattt taccttaaca gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa
     3541 aattcacatc gaataaaaat attttaggac aagcacaggc gacctcttac gcacttgtgc
     3601 catttaattt gaatcatgaa ttctatggag cacttgtagt tagtcaacct gcgcgaaata
     3661 tcgataacag cgttcgtcca attacactga acctgttcaa aggatttatt ttttccagta
     3721 tcgttgcagt tattgccagc tatgcttttg cagcattcca agttaagcgt atcaatcggt
     3781 taaggaatgc gacgaaagaa gtgacgaatg gtaattttga tgtacagctc cccgtacatg
     3841 acaaagatga atttgatgag ttagcagatg atttcaataa aatgacgaat tcattgaaag
     3901 aatctcaagc agaaattgaa gaacaagaaa atcgtcgaag acaatttatg gcagatgctt
     3961 ctcacgagat gagaacacct cttacgacaa tcaacggact tctggaaggg ttagaataca
     4021 atgcaatccc tgaaaatcag cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc
     4081 gtctgatccg tttggtgaat gaaaacttgg attatgaaaa aatccgtacg aatcaaatca
     4141 gtatggtcat taaaaaattc aatggaacag aaacattgaa aaatattgtt gcacagttag
     4201 aagctaaagc agaagcagca ggagatacat tgacattgaa agccgttgat gatattgatg
     4261 tttacgctga ttatgaccga tttgtccaaa tcatggtcaa tattattcaa aacgcgatac
     4321 agtttactga aaacggccaa atcacgatta ctttagaaaa aggctatctt gaaacgatca
     4381 tcacgattga agatacggga attggaatgt ctgaacaaca aatgaaaagt atctgggatc
     4441 gttattataa agtagatcct tcaagaaaaa atacaaaata tggtgaatca ggattaggtt
     4501 taccaatcgt gcagcaactt gtacgcttgc ataaaggaaa attagaagta gaaagcgaac
     4561 taggaaaagg aacaaaattc gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa
     4621 aaacaagcgg tataaaagat catgaatcga tcttctatac cgcttgtttt ttactataaa
     4681 atgattgatt tagcccgcgg gctctaaacg atcaatataa ggactttgag caatagtagc
     4741 atcatgagta aagagtaaga taccttgatt ttcttcatga gctaaataag aaagcaagtg
     4801 gagtagctct tggatctcca atacactaag ttcatcaaat gtatcttcaa taacaatatt
     4861 gtttcgcttg gaaagcaagg catgaagtaa ttgcagcttt actttttcaa aagacgtcaa
     4921 ctcagctgcc gcacaattaa gcaaggaagg agagatatca agtttttctt gataggccaa
     4981 acgatcttgt ttgatttctt tttccggtac atccaacagg acatggtctt taagattcag
     5041 ataaggaaac atcggccaat ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag
     5101 aacctctttt ttcaagtgct ccaatatttt atgctgtttt tcgagaggac acaaaataac
     5161 ggtcaagtgt tcagcatgaa tcaaatattc aggtttcatt acgtccaggc ctcctacttt
     5221 gcaaatgatg gataaaaaaa gtgaaaggga atagactaag aatgagacag catccactga
     5281 gcccaataag agtttgggca aatcccttaa gtatatctgt gaaaaaaaga tcattttgga
     5341 aatcgatggt aaaaaaagtc tgttgatcca tcggaattgt aagtccgctt accgaagtca
     5401 agatatcacg gatatctggc agttcagtag tctttaagac aagaaaattg atcctttgaa
     5461 gaagcgcttc ataaactgac tggaagctta cgatccacaa taaaaccaag atacaacaaa
     5521 gaaagagagg aaagaacagc tgccagaaaa taaaaaggaa taatttggtt ttagaaagac
     5581 cagtcaggtg ccaattgatc aattcctgct tgttttgcct gactgaatag taaaagaaaa
     5641 agataaaaag gaaggcaaat agaaaaaata aactgaatat gccttttata taaaatcggc
     5701 tagctaattc taaagccgga agaagctctg tagatgcaga agcgccaata aatgaaagcc
     5761 gatcttggac ttgattggct aaagccgatt gcaaatggta aagagtcagg catgctatca
     5821 ataaaaaagc agaaaagctt gaaaataaac aatagagtat tgtctgcttt ttgtaataaa
     5881 ttaatcgagc agtagcatag cggattattt tcatactttt cacctctata aaaagtatag
     5941 aaaatgaata tgaataaagt atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa
     6001 atattttgta aaaagcaaga ggttgtgaag aagctgccct attctattac cgtagatgca
     6061 agcttttgaa cactgtttat tcgaaaagag gagcagcgac aataattttg tcgctgctcc
     6121 ttttaaattt attttatcat tcggaagaac ttgtggtcgt tccgtgaagt tcttggtacg
     6181 tttgcgtttt ataaagatca acagttgatt gtccgttgtg ttcactaaag acactagttg
     6241 acttgtcacc aagagatttt tcagcttctt ttaatttctc taagccattc ttgtagtcat
     6301 aatcactggt atcaattggg tctaatccac tattcgtata gaaccgaaga agatcaccat
     6361 tattgatttg gtcagaaaca gctaactgtt tatcaacttt ttctttcagt gaatcaattt
     6421 cattctgtac ttcctcagta ggttcagtaa tagcttcacc agtttcgtta ctatagatcg
     6481 taccaccgta gtaagtatat tttggtgtaa caaaatcacc atcacggaaa gaaacaagct
     6541 gttcatgatc tttcgagaat agatcttgtc ctaactgaat ataatttttt gtatctacac
     6601 ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc gccgtaagtg tgattgattc
     6661 cgccctgtgt ttgtccaggg atatggatca tataagggac acgctgcatt tgtgcattgt
     6721 cgtagtttgt ccaagtagaa cttgtttttc caactaattc agctagattt ttatttcttg
     6781 aattcgagac accgtaatgg tcaccgtaaa ggacgatgac tgaattatca tacaagccgc
     6841 tttctttcaa ataattgaag aattcttgta cggcggtatc taaataattg gctgtcgcaa
     6901 agtaaccgtt gatcgtttca tcatttgtat tggctaacgg gaatcctgct tcgtcattgg
     6961 tgaattttga ataaggataa tggttagata cagcaatgaa ttttgaataa aatggttgtt
     7021 gcagacgttc caagtattgt acggattgtt ggaaaaatgg tttatcatgc aacccgtatt
     7081 ggaacgaatt gtcatcatta acatcataat aagaagaatc aaagaaataa tcatatccta
     7141 aattcttgta tgtctcgttt cgattccaga atgtaccagc gttcccatgg aataccgcac
     7201 tagtgtaacc ttgtgtttga cttaaaatat caggtgcagc ttggaaagta ttttttccgc
     7261 ctaattgtgt gaacaaggag ccttgattca agccaaacaa tgaattttcc atcagcgttt
     7321 ccgcatcgct tgtttttcct gcttttactt gatggaaaaa gttgtcaaaa ctgaacgtgc
     7381 tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc atgctggaga ccattctcat
     7441 ctgtcaattt ataatcgatc aggaattgct ggaagctttc taaatggata taaatcacat
     7501 tttttcctct agcgagcccg aacaaatcgt catttggtgc tgcataatgt tcttttacat
     7561 aattttctac ttctttcata tcattgggac ttgcttctgc ccgcacttgg tttgtctggt
     7621 aagtttgaac accgtcataa accatgaaag cattcaagcc aagatatttg accaaataat
     7681 cacgagagaa tgttcgagtc agcaattctg gacgatcagc ttccgccata aaaagatttc
     7741 ctgagaaaat cataactgct aatgtagaga tcgcaaaagc cattcttgct cggatcgggc
     7801 gagggtccaa cttgattttt tttgcaatca gcagaacagt caagataata aaatcgatcc
     7861 aatagatcac atcatatggg cgaaacagtc gaatggcact ttctcccaga ccactcgcta
     7921 ctttcccagc acctaaaatc gtgttgatcg taatgaagtc cgtgaattct cgataataag
     7981 tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat gaccatcatc gtgatataag
     8041 caactctagg gcgtctgata taaagagcaa ttccaagtaa taacaatgta gttgcaatag
     8101 ggttgataaa caagataaag tattgaatgc cactttcgat tccgagatgg aaatcaacta
     8161 aataggcaaa aatcgttttc aaccaaagta aaacgactag taaagaaaaa aatccgagcc
     8221 tcttattcaa aaaggcaggt gtttttatac atttcaagat aatcttcctt tctgcacttc
     8281 ttaatcacag aagtgtcctg aaaccatgtt acacagctca cttattctca tttattttac
     8341 tagtatgaca atatagtgtc aattgaaacc agaagtttat gctgtttcat taaggttttc
     8401 tttaagcttc atgaaaaaat gagcagtaag acaagtccta tgttttttgt tatacttggg
     8461 aagagaaagc taaagcaggt gaaggaatga aagtaaaagt aaaaaaaaga gcaagcaatc
     8521 gattgaaaaa aggatatcca ctgatccaaa aagaagacct tgctcatccc caaaaagaaa
     8581 ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt tatcgcaaaa ggatatctag
     8641 gaaagcagaa taagggaatc ggttgggtat tgagccagca gaacatccca tttgatcaat
     8701 ctttttttga agaatttttt gtgaagtcaa aagaaaaaag aactttcttt ttttcagatg
     8761 attctactac cgcttttcgt atattcaatg gagaaggaga cgggatcggc ggtctgattg
     8821 ttgattatta tgcaggttat gctgtttttt cttggtataa cgaaacgtta tatgcacatc
     8881 gtgaattatt gattcaagct tttcgtgaaa tctacccgga aatcatagga ggctatgaga
     8941 aaattcgatt tgaatcagta aatctgcctg agagccagtt catttacgga aaagaggcac
     9001 cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc cgtttattta aatgaaggat
     9061 taatgactgg gatcttttta gaccaaaaag aagtacgggg atcattagca gaaggtttcg
     9121 cttctgggaa aacagttttg aacatgttta gttataccgg cgccttttct gttgctgccg
     9181 caatgggagg ggcaatcgaa acaacaagtg tcgatctagc aaaacgcagt ttaaaaaaaa
     9241 cacaggaaca gtttgaagtg aatggtttgg atgtcgatca gcaaaaaatc attgttatgg
     9301 acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt tttatatgac atgattgtat
     9361 tggatccacc aagttttgcc agaaataaga aaaaagtctt tcgcgtagca aaagattatg
     9421 gggaactggt caaggatagt ctggatattt tagcagaaga cgggatactt attgcgtcta
     9481 caaatgctgc gaatgtctcg atagaacaat tccaaacaat gattgaagag gaattccatg
     9541 ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct gccaaaagat ttccagacga
     9601 cggatacctt tttagaaggc aattatctga aagtgtttgt ttatcaaatt aaaaataacc
     9661 attagaagga gcgatgcaga atggttcggg taaagggatt gaatatagga agcggtagac
     9721 caaagatttg tgcgcctctt atcggggccg tccgagagga agtattacgt gaagcagttc
     9781 ttgcacgtga agcaggggcc gatttagtcg aatggcgagt cgatcattat cgtgaagttt
     9841 tttcttttga tgaagtagca gaaacattgg ttcttatcca tgagatgctt gatggactgc
     9901 cgttattatt tacgtttcgt accttagctg aaggcggaga acaagacata tcgatccgtc
     9961 agtatcgcga cctttatgaa catgtgatac acacaggatt agtagatatg gtcgatatcg
    10021 agttgttcaa agtagaaagt ctaggaaaaa gactgataga ggaaatcaag tctttaaaca
    10081 ttcctttaat tatcagtagt catgatttta aagaaacacc agcagaccct gtcttgcttt
    10141 accgattgaa tgttatggaa cattttggag ccgatatcgg aaaactggca gtaactccca
    10201 ataatgaacg agatgttttg cggttgatgg agttgactag acgggcaaat gcttttgtct
    10261 ccatgcctat tattacgatg tccatgggaa atcttggtaa aatttctcgt ctggttggcg
    10321 gaacgacggg ttccgttatg acatttggtt ccttaagcag cgattctact tctgctccag
    10381 gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt attaaatgaa ccgttgtaga
    10441 acaagtttga ggtgatattt tttggcaaag aaaaaaatag taaaaacgaa tgcaatccgt
    10501 atggtcgaac aaaaaaagat tccctataca gagcacgaat atgagtggga cgaaagccac
    10561 ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc aatctcgtat atttaaaaca
    10621 ctggttgctg tgggcaatgt gacggggcct cttgttgctg tgataccggg agaagccgaa
    10681 ttgaatctaa aaaaattagc aaaagtttca gggaataaaa aagtagaaat gctccattta
    10741 aaagatttgg aagcaacgac cggatacata cgtggtggct gctcgcctat aggtatgaaa
    10801 aaactatttc ctacttattt agatcaaatt gcagaaagct atgaacaaat cattgtctcg
    10861 gctggcagaa gagggctaca gatggagtta gcacctcaag atatctgtgc attaacgagc
    10921 ggacaatttg cagatataaa acaataaatc atacgtataa aagaaagaag gaggccggga
    10981 tgttgtttgt ccagtctcct tctttctttt ttatacatta tgctctttgc ccggcattca
    11041 ttcctgctac atgccctgta gcgaaagccg ctgtgatatt aaagccgcca gtataaccat
    11101 tgacatccag taattctccc gcaaaataca gaccatcaat gattttactc tccaatgttt
    11161 taggataaat ttcctttaga gaaacccctc cgccagttac aaatgatttc tcaataggaa
    11221 aagttttgtt gattgatagc ttaaactctt tgcatagttg aacaaaatct tgaatttgtt
    11281 tttgagaggc ttgttgtcct gtcagatgat ccatttcaag ccgctcaaga aagaaaacta
    11341 acaatcgctc aggcaatata ccatgccaag cattgaccaa gttctttttg gttgctttac
    11401 ttttttctgt caatatatga ataagctctt gtttggtttg tgtagggaaa caatccagag
    11461 aaagtgtcac tggttctccc gttttctcta attctttatt gacaaaactt gaacaacgca
    11521 gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa atccatcgta tgttcagtca
    11581 atacacgacc ttttttattt aaaacgcgta aagtgatgtc ttgtaaagaa agcccttgaa
    11641 gtgttttctc ctgaatatac ggttcatccg atatcaaggg ggactctgtc gcataaagag
    11701 gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc atctccagta gaaccagtag
    11761 atggataagt ccgtccacca gttgttaaga taatggcagg agcatggaac tcttcgaatt
    11821 ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa tttctctact cgagtagaga
    11881 atagaagcgt cacatctagt tctttcagac gattgagcaa tgcttcgata atcgtttttg
    11941 atttatttgt tacaggaaac atccgtccat gatcctcttc ttttaaatgt acgccttgtg
    12001 attcaaagaa gttcatgata tcgaaattat tccattgcgc aaacgtacta tataaaaact
    12061 ttccatttcc aggaatatgt gcaatcaaat catcaacagg acgattgttc gtaacattgc
    12121 accgaccacc acctgtcatc agtagttttt ttcctgcttt tttgtttttt tcgatcaaga
    12181 gtacgcgtgc gccttgttca gctgcactga tcgctgccat catcccgctt gttccagcgc
    12241 cgactacgat cacatcatac agtttttcca ttccctcacc tcaaggaaag tgtagcatat
    12301 ctgaagaaag aaaacagaaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
    12361 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
    12421 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
    12481 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
    12541 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
    12601 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
    12661 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
    12721 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
    12781 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
    12841 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
    12901 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
    12961 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
    13021 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
    13081 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
    13141 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
    13201 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
    13261 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
    13321 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
    13381 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
    13441 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
    13501 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgtccagcga
    13561 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
    13621 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
    13681 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
    13741 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
    13801 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
    13861 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
    13921 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
    13981 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
    14041 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
    14101 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
    14161 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
    14221 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
    14281 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
    14341 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
    14401 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
    14461 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
    14521 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
    14581 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
    14641 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
    14701 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
    14761 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
    14821 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
    14881 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
    14941 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
    15001 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
    15061 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
    15121 aagaaaaaca tgttcgcact tctaggcaaa ccaggctttg aagatcttgc taaagaattg
    15181 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
    15241 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
    15301 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
    15361 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
    15421 gttaattctg tttttttgtt ttctagaatc agtaag
//
LOCUS       DAFEHS010000065        15412 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFEHS010000065
VERSION     DAFEHS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.067"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..561)
                     /locus_tag="LP327_002096"
     CDS             complement(<1..561)
                     /locus_tag="LP327_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBL1726197.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="LP327_002097"
     CDS             complement(748..1398)
                     /locus_tag="LP327_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726198.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="LP327_002098"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="LP327_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBL1726199.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="LP327_002099"
     CDS             complement(2910..3908)
                     /locus_tag="LP327_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBL1726200.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="LP327_002100"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="LP327_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBL1726201.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="LP327_002101"
     CDS             complement(4924..5655)
                     /locus_tag="LP327_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBL1726202.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="LP327_002102"
     CDS             complement(5659..6924)
                     /locus_tag="LP327_002102"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBL1726203.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="LP327_002103"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="LP327_002103"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBL1726204.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="LP327_002104"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="LP327_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBL1726205.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="LP327_002105"
     CDS             12266..13768
                     /locus_tag="LP327_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBL1726206.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="LP327_002106"
     CDS             13770..14507
                     /locus_tag="LP327_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL1726207.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14552..14725)
                     /locus_tag="LP327_002107"
     CDS             complement(14552..14725)
                     /locus_tag="LP327_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726208.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14701..15366
                     /locus_tag="LP327_002108"
     CDS             14701..15366
                     /locus_tag="LP327_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726209.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
BASE COUNT         4540 a         3298 c         2603 g         4971 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga cacttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
    14521 tgcacggtcg ccatgaaggg accgtgtatt tttatcttct tattttctca cctaatcgta
    14581 tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
    14641 aagaaagtac catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta
    14701 attgagcttg atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag
    14761 caattgtgtc aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat
    14821 tacggacagg ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt
    14881 tccagagcct cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat
    14941 cagttacagc taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa
    15001 tctttccttc atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag
    15061 atccgctttt ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa
    15121 cgaacattat atgtaaaacg tattttgaaa agcctgacag aagaagaagt acatttgttc
    15181 aagaagactt tggcatgctt cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa
    15241 ttatttattc ataaaaacac cttgcaatat cgattgaaca agttccattc tctcactggt
    15301 tataccccta gaaactatga cgattatcat attctaaagc tggctttttt gttggtacaa
    15361 acatgacaac tacaaaaaaa ccaggcgaac atatgattgt tcgtctggtt tt
//
LOCUS       DAFEHS010000066        15259 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFEHS010000066
VERSION     DAFEHS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.068"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="LP327_002109"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="LP327_002109"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBL1726210.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="LP327_002110"
     CDS             951..1256
                     /locus_tag="LP327_002110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBL1726211.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="LP327_002111"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="LP327_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBL1726212.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="LP327_002112"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="LP327_002112"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL1726213.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="LP327_002113"
     CDS             4416..5354
                     /locus_tag="LP327_002113"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBL1726214.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="LP327_002114"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="LP327_002114"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBL1726215.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="LP327_002115"
     CDS             6725..7465
                     /locus_tag="LP327_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBL1726216.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="LP327_002116"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="LP327_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBL1726217.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="LP327_002117"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="LP327_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBL1726218.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="LP327_002118"
     CDS             10608..11258
                     /locus_tag="LP327_002118"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL1726219.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="LP327_002119"
     CDS             11259..11897
                     /locus_tag="LP327_002119"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBL1726220.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="LP327_002120"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="LP327_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBL1726221.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="LP327_002121"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="LP327_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBL1726222.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13674..15248
                     /locus_tag="LP327_002122"
     CDS             13674..15248
                     /locus_tag="LP327_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL1726223.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5116 a         2864 c         3318 g         3961 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    13681 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    13741 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    13801 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    13861 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    13921 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    13981 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    14041 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    14101 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    14161 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    14221 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    14281 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    14341 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    14401 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    14461 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    14521 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    14581 caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca acagaagaat
    14641 caacaacagc gcctgaatct tcagcaacag aagaatcaac aacagtgcct gaatcttcag
    14701 caacagaaga atcaacaaca gtgcctgaat cttcaacaac agaagaatca acaacacctg
    14761 cgcctacaac accatcaaca gatcaaagtg ttgatacagg aaacggcaca ggaagtagta
    14821 ctccggctcc aacgccaaca ccaacacctg aacaaccaaa acctgtaaca cctgctccag
    14881 ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga agcttacaaa tatattggta
    14941 ctccatatgt ttggggcggt aaagatccaa gtggctttga ctgctcaggg ttcacacgct
    15001 atgtttacat gcaagtaact ggtcgtgaca tcggtggttg gacagttcct caagaatcag
    15061 ctggaacaaa aatttcagtt tctcaagcaa aagctggaga cttgttattc tgggggtcac
    15121 aaggcggaac ttaccacgta gcgattgctt taggcggagg acaatatatc catgctcctc
    15181 aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt tgcccctgac tttgctgtca
    15241 gcatgtaaga atgattaat
//
LOCUS       DAFEHS010000067        15230 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFEHS010000067
VERSION     DAFEHS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.069"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            278..787
                     /locus_tag="LP327_002123"
     CDS             278..787
                     /locus_tag="LP327_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL1726224.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="LP327_002124"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="LP327_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL1726225.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="LP327_002125"
     CDS             2322..3302
                     /locus_tag="LP327_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1726226.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="LP327_002126"
     CDS             3424..4842
                     /locus_tag="LP327_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBL1726227.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="LP327_002127"
     CDS             4913..6382
                     /locus_tag="LP327_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL1726228.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="LP327_002128"
     CDS             6392..6859
                     /locus_tag="LP327_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL1726229.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="LP327_002129"
     CDS             6895..7767
                     /locus_tag="LP327_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL1726230.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="LP327_002130"
     CDS             7848..10382
                     /locus_tag="LP327_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL1726231.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="LP327_002131"
     CDS             10538..11311
                     /locus_tag="LP327_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL1726232.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="LP327_002132"
     CDS             11304..12095
                     /locus_tag="LP327_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL1726233.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="LP327_002133"
     CDS             12105..12596
                     /locus_tag="LP327_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1726234.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="LP327_002134"
     CDS             12614..13024
                     /locus_tag="LP327_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL1726235.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="LP327_002135"
     CDS             13028..14428
                     /locus_tag="LP327_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBL1726236.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="LP327_002136"
     CDS             14451..14825
                     /locus_tag="LP327_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL1726237.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DAFEHS010000068        14641 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFEHS010000068
VERSION     DAFEHS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.070"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..625)
                     /locus_tag="LP327_002137"
     CDS             complement(<1..625)
                     /locus_tag="LP327_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL1726238.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKN"
     gene            complement(627..1391)
                     /locus_tag="LP327_002138"
     CDS             complement(627..1391)
                     /locus_tag="LP327_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBL1726239.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1399..2076)
                     /locus_tag="LP327_002139"
     CDS             complement(1399..2076)
                     /locus_tag="LP327_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBL1726240.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2113..3498)
                     /locus_tag="LP327_002140"
     CDS             complement(2113..3498)
                     /locus_tag="LP327_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL1726241.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3708..3792)
                     /gene="ffs"
                     /locus_tag="LP327_002141"
     ncRNA           complement(3708..3792)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LP327_002141"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3815..4330)
                     /gene="tadA"
                     /locus_tag="LP327_002142"
     CDS             complement(3815..4330)
                     /gene="tadA"
                     /locus_tag="LP327_002142"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBL1726242.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4437..5294
                     /locus_tag="LP327_002143"
     CDS             4437..5294
                     /locus_tag="LP327_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726243.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5375..5710)
                     /locus_tag="LP327_002144"
     CDS             complement(5375..5710)
                     /locus_tag="LP327_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726244.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5777..7147)
                     /locus_tag="LP327_002145"
     CDS             complement(5777..7147)
                     /locus_tag="LP327_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBL1726245.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7225..7761)
                     /locus_tag="LP327_002146"
     CDS             complement(7225..7761)
                     /locus_tag="LP327_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL1726246.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8070..9551
                     /locus_tag="LP327_002147"
     CDS             8070..9551
                     /locus_tag="LP327_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726247.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(9654..10361)
                     /locus_tag="LP327_002148"
     CDS             complement(9654..10361)
                     /locus_tag="LP327_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBL1726248.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(10440..10778)
                     /locus_tag="LP327_002149"
     CDS             complement(10440..10778)
                     /locus_tag="LP327_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBL1726249.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(10889..11851)
                     /locus_tag="LP327_002150"
     CDS             complement(10889..11851)
                     /locus_tag="LP327_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBL1726250.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(11866..13197)
                     /locus_tag="LP327_002151"
     CDS             complement(11866..13197)
                     /locus_tag="LP327_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL1726251.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(13205..13885)
                     /locus_tag="LP327_002152"
     CDS             complement(13205..13885)
                     /locus_tag="LP327_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBL1726252.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(14104..14583)
                     /locus_tag="LP327_002153"
     CDS             complement(14104..14583)
                     /locus_tag="LP327_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBL1726253.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
BASE COUNT         4274 a         3107 c         2382 g         4878 t
ORIGIN      
        1 tattttttcc taagaaaaga aaaacagcaa aaaataattc taataataca aaaaataaga
       61 taaagctatg catgaaaaat tcttctggaa gtacattgat gattggatca gtaacgggat
      121 cgaataacca gtcatcgttg ttgaaaaacg tttcatgaaa aaggataaaa aagcgatcaa
      181 atcctatcat catgaaaaaa ccgaaaacaa ctggcagtaa catgccgatt tgaaacggac
      241 gaatcaaccg ccaaagccga tcattctttt gcaaatattt gataaacaaa atactcggga
      301 tcaaagtgat gaaaaacaca gcataatcca ctaaaaacaa aattttcact tcataaaagt
      361 gatgggctcc gctagcagaa actgggaaat ctggcaagct gaggatggtt ttgaagggat
      421 tattgagata attcattagg tgatcaaaat ttttcagtaa cgtctcttga tctaaagaag
      481 tataatccaa aatttgcaaa tgtccgatat caaatacgta tagtggtcga aagttgatcg
      541 tcacaccaac tgccagactg atcagtgtca gaaataggct gaacaatcca gcatattcca
      601 tccactgcca tctcgaattt ttcatattag aagctccatt catccagtga ttgtttcaca
      661 taggtagctg ggataggcaa cccaggtaca tcttcttctt ttgtaaaacc tgataaaacg
      721 agcaaagtat ctatattatt gcgtatccca gcttggatat ctgtttcata gttatctcca
      781 accatgatta cttcttcttt ttgcagccct aatacttcaa cggctttatc cataatgatg
      841 gcttctggtt ttccgatgta gatcggcggc gtttgggttg ctgtctctac aaaagagatg
      901 actgaccctg ctccaggaag aaggccgcgt tcagtaggta tattcttatc tgggttcgtt
      961 ccaatgaaag tagcaccttt ttgaatagca agtgttgctt taactacttt ttcataggtc
     1021 agataattat ctaagcctac cactacatag tcaggagcct cttcttccca tgtaaatccg
     1081 gcagctaaaa tcaagtcaat caatcctgct tctccgataa catagacttt tttccctttt
     1141 ccatctgctt tcataaaatc gattgttgcc aaagtagctg tgtagactgt ctctggagct
     1201 acatgaatgt caaactcatc tgctagacga ttagctactg tttctggtga cttagtcgtg
     1261 ttattggtaa caaacaagaa aggtaatttt cttttttgca attcttccac aaatcgttta
     1321 ccggccggta tgggttgtgt cccacgataa atcgttccgt ctaaatcaat taaatatcct
     1381 ttatagtcca ttcatccact actcttccct ttttctgatc gtaaatgctg tttttgtttt
     1441 ttctacttgt ttaggttgtt cttgtttttt tactttttca gaggtttttg ctgactctgt
     1501 ttttcgattt ttaataaccg gttttttgcg attagatgcg actggtcctt ttttctcatc
     1561 gatatacgca cgattatggt tttttttctt ttttcggcgc tgagttgtct tttctttttt
     1621 accgccgatt ctctcgatga caaaatacgc acaaccaaaa ttgcaatatt cgtaaagata
     1681 gtcttccaag gaatcgattc gctgatctgg atgcgcccgt cgattatccg cttggaaaaa
     1741 tccttttagg cgtagttgtt cataacccca atctcctacg atataatcat atcgggctag
     1801 tacatcactg taacgttctc caagcttttc tgcattaaat ccttcacgat aatcggacac
     1861 aagccgataa tgtctttcac ctatcaaaaa attactgtca tctaacagtg tcactagctc
     1921 gcctttgact ttttctgctt taggctcctc aacgatttct tccaaagcag ctgtcagttc
     1981 ttcggtgact gttgtttcct gttcagctga tgattttttc ttagcaattt ctgttttatg
     2041 ctcaacgttc tgctgttcat tttgttttgc agacatgcgt tcaccaccta tcttttttat
     2101 tataccttgt ttttatttga ttgggtagtg agctttcaag taatctccta ccacacttcg
     2161 gataaattca gggaacaaaa attcattttt tccagcaatc tcaatcgttg gaaaaaatgg
     2221 aacaaacatc aaatgatcta cggtagtaaa agtataattt ttattcagat cgattggctt
     2281 gttcttccaa aagacttgat ggttaataga atcatatgtg attccattat agaccatctg
     2341 cccaaaaatc ttaccgcgaa atcccattcc cttcatagga tagttacgta aaaagttacg
     2401 atttttttct atttctaaaa ctaagcggat cagatcattt ccacccaatg tgacatttaa
     2461 caagtgcatc ggatgcggca acgcagtgtg aagctgatcc tgattgaccc gcccttttgg
     2521 aatttcagtt aaaaacagac cactgtttaa tattgctgct tccgtctttc ctcgttcttt
     2581 caccgcctca aaggctactt ggataaacga atgctcattg aagatatcta gactcagaga
     2641 aaatggcaaa tctgctactt ctttttcttt taataattca tgcccacgtt ctagataact
     2701 atttatttct tgttcatctt ctgtaaaagc tgtcattgtt tcggtaggga tggctttggc
     2761 tgaatgctta aggatttttt tctcttcatc gatggttaaa tggacttcac cgacatactg
     2821 cccgtacttt ccagcggctg ctatttggac atcattgatt acgtgacctt ctctgaataa
     2881 atgatgggtg tgcgatccta aaataacatc aattgctggt atttcttttg caatttggaa
     2941 atcatcctga atcccgagat gactcatcaa aactaaaaca tccacttgtg aacgtaaatc
     3001 ttcgactaat tctggcaaaa tatcataagg attccgtatg tcccagccat tcggattata
     3061 agtaagcgga aatggagcag taaatgcaat tagtccgatt ttcgtctggt gtttcgttgt
     3121 aatgatttta tatggtttcg tccatgccgg tggctggagc gtatttttat cgaataagtt
     3181 atccaggatg atatcaaact cagcatgatc gtataaatga ttcaactctt ctttggaatt
     3241 tcctacgcct tcattgtttc ctatcgtcgc tgcatcataa ccaacttcat tcatcagcaa
     3301 gatattttcc tgtccattcg ttgcttctga caatggatgc caacgatcca caaaatcacc
     3361 tagatctact gcaatcgttg tcgttttttc tttcttttcg ttttctcgtt tacgttgatc
     3421 caaaaacctt ctgatccttg gccaattttc taaatgagaa tgcagatcat tcgtatgtaa
     3481 aatcactatt ttttccatgt accctctcgc ctctctttat ctttatcata ccataggcag
     3541 ctttttcttc cattgaaaca agctctattt cagagttttt cctgccttga agcaaattgt
     3601 ataagtacaa tattcgataa atcagtctgg ctaaaaaatc aggaacattc atttttacag
     3661 tactataaaa ataaaaaaaa cagacccgaa agtctgttgt cgtttcatta taatcagaca
     3721 catggcggaa cctgcttagt gctgcttcct tccagacctg acacgcttca caagcccacc
     3781 attgtcctag cctttcggca aagaatagta taccttattt ttcattcgtt gaccagtctt
     3841 tttttaattt cttttttcgc tggcggagct ttcggaaaaa gtcagtcaaa atcatcccac
     3901 attcgtcttc taaaatgcca ccttctacat atgcttggtg gttaaaccgc tcgtcctcca
     3961 aaagattcat caaggtaccg gctgttccgc cttttggatc atacgcgccg aaataaactt
     4021 ctgctacacg agacaaaatc atcgcgccgc tgcacatagg acaaggctct aaggtaacaa
     4081 aaagtgcagc atcttccaag cgccagcttc ccactttttc acaggcttcc ttgattgcaa
     4141 gcatttctgc atgtgtagta gcatcttgcg tggtttctct aaggttatat cctcttccaa
     4201 tcacttttcc atccaataca accactgctc caatcggaac ttcatgaagc atttctgctt
     4261 tctttgcttc tgccaatgca aggcgcatcc atttttcttt ttctactgca tcaagatttg
     4321 tatttaccaa ttacccttcc ctgcctttgt tttttcttgt tttactttag catatgataa
     4381 aaaggctgta actaactttt ttttctatgg taaaataaat acaagaggtg aacaagatga
     4441 aaatggataa attacttgct ctttacccac aagcacagaa aaaaaactct ccagcatcag
     4501 aagcggatat cctttctttt gcaattgatg atgcttttat ctggatcaaa caagattcat
     4561 tatctcaaca agaaaccagc cttttgaaag cgttattccc tgtaatcaac gatcaaaaaa
     4621 aacacccatg gtatcactat ctttttaaag atgcttcttg tgttcaagaa aaaagctttc
     4681 gtttgatcca gcttcacgtc gaatcaaaag gagaattctt aaaaaaagaa tggcaagata
     4741 ctattttgga attatttcgc tattctgctg atttcttttt ttatacagac acagatgcgc
     4801 tcttagtcga acaaataaac cgtgattatc ttgatgctac tgaaatcaac ggtatttttc
     4861 tatctttaga tgctgatttt gatacgacaa cgtctgtatt cgttggttct ttccattcac
     4921 ctggcaccga cctcgtccct ttgtttgctg aagaaagaaa aattttttta acagagcaaa
     4981 acaatcttta tactcacagc cgtacttttt ctatgcaaga tgtggcttta cattactata
     5041 caaaagatac tgtaaaagat agtaagttga tcaaaagtta catagaactg atcaaacaaa
     5101 atgaaatgga agtgatcatt caagaattat ggaatgaatt aggcaatgtc agctctgctg
     5161 caaaaacact gtttcttcat cgtaatactc taaaatatcg aatcgaaaaa tttcaagaac
     5221 agtctggttt caatctaaaa aaagccaacg atttactgtt ttgctatctt ttattgcttc
     5281 atgaacataa ttaaagatag attaaagggg ttgtgacaat aattttgtca caacccctta
     5341 tttcgaatga atagtgttca aaagctagtt tctcttacat cattttttca acaaaatgca
     5401 ttagccggtc ttttacatct tcggttgaat ctttgattga atcaccataa acagagattt
     5461 tcttttttcc tgatttgatt tttgctaaca ttcccatcaa agaatccaag tcactatctg
     5521 aaagaccatc tacgatttct agtaattttt catttccatc aaatttgtca tagacgaatt
     5581 tttttacttt cgtccgagta gtcatatgat ggatcttttc cattactttt cccgatacaa
     5641 cgactgctgt cgctacactg gcagcggctg ctacgcttaa tccgattgtt acttttgttg
     5701 atgttttcat aagtatcagc tcctttttat ctatctccat catagcatag acaaaaaaat
     5761 aaacgaacta atccactcag tgaaatctca atcctttagg gaaaaagttc tttactgttc
     5821 cttttacttg tttcccaaat ccgacaggta tcttatctaa tactagcaaa acccatcctt
     5881 gattcccatc acgttgaaat gtttctcctg ctgtgtaaga ttgccattcc ttttgagtga
     5941 tcggcaagca aggtatattc tctatctttt ttgtagcaag cgctaatgca tagctaggtt
     6001 caaatcgatt ctttttgaaa tctcctaaat gcaaacctgt acgtacgact tttaaaccat
     6061 cgagagaagg ggctaactca ggaacttccc aaagatggtc gttgaacact aaaagtcttc
     6121 cagtagcttc ataatgaaaa tcatttgaaa actccgtcca gagtttttcc tgctcttttg
     6181 tcatttgcac tttacttttt tttcgtgtct tcttttcttt atgcatctga ttttgtccat
     6241 gaaaagtcag ctttgcaaca aaatgtcctt caccctgatc tttatgaggc catatacgta
     6301 ttgttttttc caaaccagct acgcttcccc attcagaacg tcctgaacta acggattgag
     6361 tcaaaggaat ctcctcaatc gttactggat aattttctac aagccagcta atgatctctt
     6421 cattttcttc tggtgcaaac gtacaagtcg aataaatcag ttgtccttta ttttttagca
     6481 tttttattgc tgaagacaaa atctcttgct gacgtttttg acagtatagc ggagattctt
     6541 ctgtccactc ttttattgca tttggatctt ttcggaacat tccttctcct gaacaaggag
     6601 catcaaccac gatccgatca aaaaagcccg aaaaatgagg gacaagttct gcgggtgcat
     6661 gatttgtcac tattgcgttc gaaactcccc atcgttctat attttctgat aaaatcttcg
     6721 cacgtttcgg aaagatctca ttcgtcacta ataacccttt ccccttcatt tgagcagcta
     6781 attgggttga tttaccaccg ggagccgcac acaaatccaa aactttttca ccgggttttg
     6841 ctgcagcagc tgttccaacg atcattgcac ttggttcttg gctatattca tacccagctt
     6901 gatggaggaa agactttcca ttgacagtgc caagaaatcc ctcattgctg tagggagcgg
     6961 gttgtaattc ttcgctatgg taagtttgga ccatgtccaa tcctgccggt tttagtggat
     7021 tccaccgaaa tccttttttc acgcttcctt gttcaagcgc agaaaaaaaa tcggatgctt
     7081 cttcgcctaa aagaagacga tattttttga taaattgctg tggtaacgtt gtcgcttcct
     7141 ccttcattcc aatgtttcgt tagacatttt acaatctttt ttacgaaaaa taaaggtggc
     7201 tgatctactt tttcatgttg ctgcctaagg tgactgttcc tttaagatat tttccaagta
     7261 ttcatccgtc acctcgtttt tatcccaaac agcgttcatc atttcaagaa aatcttgaga
     7321 agaatcatct gagcgcaaac ttcccaatgc attaagccat accaattcaa gcgacttttc
     7381 ttctttgatc ttcgtgtgac ctttgacata tgagaaaaat ggccgcttaa atagcgaatc
     7441 catgtctaaa tcagattttg caatgatttc acgaatactt tcatggatag aaaaaaaatc
     7501 tttcaataag tagacaatgc ttaaaacatc atcggaactg cattcagcta agtagttttc
     7561 aaaataaaca gtgatttctt ctaatatctt ctcttttaac ccgctgattg atttgaaatg
     7621 tgtataaatc gggtatgtag aacaaccgca gaactctgct aactctcttg cagtaattga
     7681 cttcattcca tattcttcca cgtataccat acttttgaac agcaaatatt ctttactata
     7741 atttgttact ctccccatca ttcttacctc cgtctctttt catctaccca taaataaata
     7801 acataaataa ttctaaaaat attattgata attatcaaaa catatttagt tatatccatt
     7861 aaaaatcaat ttttccgcct taaaaacaag aatatcttga caaaataccc taggaaataa
     7921 acaatagaat aaacaaggta acaaaaaaat taaataacgt tatataaaaa agaaggcgac
     7981 actctgtgag aataacacag actgaaatga ctttccactc acagtttttg tctaactata
     8041 tacaaaaaca gaataaaaag gaggtattta tgctaattga agcaagaaaa aaagagttac
     8101 ttgttctaca aatggtcatt caaaaaaata attgttcttt acaagaatta tccaatgact
     8161 tacatattcc aaaacggaca atcaaagaac tgatccgaaa aataaatgta tcactcaagc
     8221 aatttttatt gattgatgaa tttatttatt ctaatcataa aggagagatc caaaccaaag
     8281 aaaccgatca aactaaaaag ttagctgttt tttctaagtt aaaattatgc tacctaaaag
     8341 aatccaatcg ttttaactat ttgatgttac taatcaatta tccacaaact gctgtaccaa
     8401 aaaaatattt acttgaacaa ctttatatat cgccctccta tctcgaaaaa ctgacaaaac
     8461 agctcaatca ttccttaaaa aaattccatt tacggatcct atctgtaaaa aattgctgta
     8521 ctcttgaagg aaatgagtta tctatccgtc tctatcttta cttttttctt tcagatacat
     8581 ttggaggtaa cgattggcca ttttcctcac ttgaattaaa tgcactaaaa atgaacagta
     8641 cagatgagca aatagaaaaa atttcaaaac aagaaaaaaa tatctacttg ctgttaactt
     8701 tattcatatt acgaaacaaa caacaggcat attttccaaa aacttcttct gaaatcactg
     8761 aattgatgga cttattgcag catacacatg attattcaga atactttact tctttttcag
     8821 tgagtcgccc ttgtgaagag acagaaacag agagagccta ctttaattat tttttctatt
     8881 gtttttttcc aaatcaaatc tcttttcgac aaaaacagga gattggtttt ttattttcca
     8941 caagcaaaaa ccttatttgt cgacaagtcg gtcaattgat gacttactta actcaacagt
     9001 ttcctctcaa ccatttagaa aataaaatac caatttattt ttattttctt gtgttggcag
     9061 ttgttttttt cgaactgttg ggtgaatcca gcagtagttt ttctacactt tgctttccag
     9121 aagccacgtc caatcattct agttctttta tacaggaaaa gaaattaaga gaaaaaatcg
     9181 atacaatttt ttcaaaaaat ggggaaagaa accaaatcag tcaatacctt agtcaattga
     9241 tccaacactt actacaaaca gaaacgaaag agcagttact catccatttg cagataacca
     9301 aacagtttac aggaaattat catcttcaaa gccatttgca gcagttattt cgcaaagatg
     9361 ctgttaaaat taccgcatat tcagaacatg ctgatttgat cattacagat cagcatgaga
     9421 aaatttcaga tgaaacggag actttctatc tggatacaac gcacaataca gtgttatttg
     9481 gcaagttaat aaaagcggtt catgaactgt atatgaaaaa aggttatgag aacaaaacaa
     9541 agaggatgtg acaaaattct tgtcacatcc tcttattcca aataaatggt attcaatcag
     9601 ctaactctcg gtattagctc agatcaaagc gctgatttca caacttcttt attttaatct
     9661 cttcggttgc cgccgaacaa taagatcaag cgcaacagct gcaggaatgt cgaaagcgct
     9721 gctgcaacat aagtcagtgc tgctgcaaat aatacgtgtc ttgccatcgg tacttcttct
     9781 tccgtcagta gtccgccttc actcaaaatt tggattgctc gccgagacgc gttaaattcg
     9841 actggtaatg tcaccaattg gaaaagtaat gctaaagaaa aagcgaaaat cccgatattg
     9901 attaacgtag agttccagct aagcaataca cctaccataa tcaaggggaa cgaaatcatc
     9961 gaaccaaagt tagctgctgg aacaagtgct gcacgaatct tcaatgggac ataacccgaa
    10021 gcatcttgta ccgcatgtcc gcattcatga gctgctacac ctatcgcagc aactgaagta
    10081 gactgtgcag ttgcttgaga caaactcaac atcttattac ctgaattgta gttatctgtg
    10141 agatctccgg caatttgttg aacaccgaca ttatttatct gttgactttg taatatatat
    10201 tgtgctgctt gtgtaccagt cacattgttt ttgcttctca cttgatcgta ttttcggaaa
    10261 gtactattga cgtacgctga cgcaattccg gaaataacta atccaagaat gaccaaaaga
    10321 aatgtcggat ctaaaaaacc atacattcca taaccataca ttttcgtttc actccttttc
    10381 gtttacttta ttttatcatt agtagtagga aaagaatgtg aaaagacctt gaatattctt
    10441 taattctttt tcacaaattc cgatttcagt ttcatcggtc caaatccttc taccttgcaa
    10501 tcgatattat gatcgccttc tactaaacgg atgttcttga cttttgttcc ttgtttaatg
    10561 gcacctgacg caccttttac tttcaagtct ttaatcactg tcacgctatc gccctctaac
    10621 agacggtttc catttgcatc taataccact aatccgtctt ctatgacagt ttcttgttct
    10681 tctttgttcc attcgttccc gcattctggg caaatcaaca agcctcgatc ttcatatgtg
    10741 tactctgagc cacattctgg gcaatttggt aatgtcatct tctttctcct ttaaaatctt
    10801 tagtgagcac tattgtaacg gaaaaagaag atcttgaaaa gatacatttg tgatttttac
    10861 tacaaaaggc acgttttcga acgaagtatc attctgtttt tctgctctta tacgattggc
    10921 atatatcttg gatctcttta tagatttcct ctacttctgc taaatctttt gcattcgcac
    10981 cacttgctaa tggatggcct cctccatgat ggcgtttagc caattcattg atgatcggcc
    11041 ccttagaacg taaacgtacg cggtaatacc ctgaaggctg ctgaacaaaa atcccccaag
    11101 ccagcacttc gtcaatcact ccaggaagtg aaacgatcga agctgtctct gaatcatcga
    11161 ttccatattt atcaagaatt tcttgcggaa ggattattct tcctgcacca ttttcatcca
    11221 cctggatatt ttgataaaga taaccggaaa gtttagcgac tttcatcggc atttgttcaa
    11281 tctctctgtt caaagcactt gcatcaaatg gataatttct caatatcgaa gcaatctcta
    11341 atgtacggga cgtcgtggag ggatacaaaa agcgccctgt atctccgacg attcctgcat
    11401 aaagtaaccg tgcagcctta acgttcatct ttaaagattc aggaaacatc aaagcaaatt
    11461 ctgcaatcat ctcactacag ctacttgctt ttgtatttac ccacacaaga tctccataag
    11521 ggtcatcatt gggatgatgg tcgattttga tcagtttttc tgccaactga tatctttgat
    11581 cactgattct gggtgaatta gctgtatcag tcacgatgac tagcgctcct tggtagaaga
    11641 catcttcaac ttcttgcatt tctgctaaaa agcgaagccc ttcaatcgtt tcacctactt
    11701 gacgtatttc tttttctggg aaactttccc gtaatagttc ggctaatcct acttgtgatc
    11761 ctaatgcatc aggatcaggg cgcaaatgcc tgtggataat gattttatcg tattttttta
    11821 ttgtatctac tatttcttgc tgtactgtca tttcactcct gctttctaag aacgttccat
    11881 tacttgacaa acaacgattg ctttggaaac gatggtgttt tcaataaaca cttcgatatc
    11941 cagtttagcg gaacgtctgc cgatttccaa tacacgtgga cgaatgtcta attcgctttc
    12001 caattggatc agccgcaaat aatgtaggtt cacttgttca atcaagatat ttcgtttttg
    12061 gttcatcacc attgtttttt gtgcgacatc agaaatgatt tcgctaagta cgccaaaaga
    12121 aatggttcca acgccattga ccatttgtgg tgctactacg aatttgaact tagggttttc
    12181 caaacgattt ccttcacggt cagtatcaat cgtatggata tttccagata tctgatccga
    12241 aatcgtatct gcaatctgcg gttgtcgctg gacaagctgc atcgctttca ttacatcttg
    12301 ccgggtaatc aagccttcca atgtcaagtc atcggaaacg accggcatga cttctagccc
    12361 atcccagatc atttgatggc taacagaggc aatactcatt gttttcttta cactgattgg
    12421 ttctttcgtc atcacccgat cgatgatctg gtttggcgat ttccctaaaa catcttttgc
    12481 cgtaacaatc cccatcaaac gatgatggcg gttgacgact ggaaaacgcg aatgctgcgt
    12541 ttcttctgac aaacgctgat aatcagaaat cgtatctgtc tgctgcaaat aatgagtctt
    12601 ttcagctggc atatagatat cactgacaag catgatatct tttttgatca attggtcgct
    12661 taacgctcgg ttgatcatgg ttgcgactgt aaatgtatca taagaggtgc gtaagatcgg
    12721 catttcaaga tcatcagcta gctttgcaat ctcagcagtc gtattgaatc ctcctgtgat
    12781 caatactgcg gcgccatctt ccaatgctaa tttttggact tccgcccggt tgccgacgat
    12841 cattagtgat cctggcgtaa tatagcgttc catggcatgt ttagtcatcg cgccgatgac
    12901 gaatttattc aagaccttgt ctaatcctgc cgccccgcct aaaacgtccc cttcaatgat
    12961 cctgacgacc tcgccaaatg tcaaacgttc aatatgtttt tttaatttgc gttcgatacg
    13021 gatggtccct actcgttgaa ttgttgaaac tagtccgaca ttttctgcat ctttgattgc
    13081 acggtacgct gttccttcac tgacattcaa ttcttttgcg atgcttctta cagaaattcg
    13141 attgccaaca ggtaaacttt caatatattc taaaatcaaa tcatgttttg tcgccatttt
    13201 ctgcctacac ttccagcgtt tccgagacgc tcattatttt tccttcacta tcttccagta
    13261 actctacaaa agattcggga tcttgttcaa tgacagggaa agtattgtaa tggattggaa
    13321 ctactttctt ggcacgaatt gtttgcgcag cctttgctgc atcttctggc cccatcgtat
    13381 agttatcgcc aatcggcaag aaagcgacat caatatcgaa ttcttctgca agcaatccta
    13441 gatcactgaa ataagcagta tcccctgcat gataaatcgt ttttccttct gcttgaataa
    13501 taattccgct aggctcgccc atatactgaa cgataccgtc tacttcatag cttgaactat
    13561 gctgtgcatg tacgaatttt accacgccga atggaaacgc atgttttcca ccgatattca
    13621 taccgaatga atctactcct ctgctgttcg cataatcagc aatctcaaca atactgataa
    13681 ttgtggcttg attttctttt gcaataggaa tcatatctcc tatgtggtca ctatgaccat
    13741 gagtaatcag caaccaatct gtctttactt cttctgcagt taagtctgtc aatggatttc
    13801 ctgtaataaa cgggtcaatc atcacgcgtt ggccattttc catttcaatt gttaaacagg
    13861 aatgtccatg atacgtaatt ttcatatttt acacctcgcg ttttctctgt atctttttta
    13921 ttaaaaacga tttcatcact tgcacatcaa tgaaaaaata tagctgctaa atatattttc
    13981 attggattga tagtacagta tgtacaaagc aattgtaaca gataatacag gatgcaccaa
    14041 gtaccaagca gatataatct caagtttgag gtctctgctc tgcttagctt ctcttctcgt
    14101 cccttaacta attaaatcag cgtaaatagg atggacgatc ttttctaaat cggctacttt
    14161 tatccgaagg cttctcccga tttcaccgct tgacacaatt acttcttctt tttcttttaa
    14221 agaactgtct gctaaaaaac aataatcagg atgtgcgagt tttattccaa taggggtgtt
    14281 tgctccgtgg ggatatcctg tatgcattaa tacaaaatcc attccaggaa aaccaacttt
    14341 acgatctttc gtcacttgtc tcgttttttt gtaatctaag tgatcgttca aaggaatcaa
    14401 tgcgatgatc accccagttt tgtttccttt cactacgaga gttttacata tcaaagctgg
    14461 atcaatccct cttttttgta gttcttctgt gaaagattct tctaccacat gactaaaatc
    14521 aagtggttga tagctgatct tttgttcttc taaataacgt tcgacttttg tttgactcat
    14581 cattgattca ctccctttat tctatcgttt attataaagc gagggatgct gtattcaaaa
    14641 a
//
LOCUS       DAFEHS010000069        14118 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFEHS010000069
VERSION     DAFEHS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.071"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..556)
                     /locus_tag="LP327_002154"
     CDS             complement(<1..556)
                     /locus_tag="LP327_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBL1726254.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="LP327_002155"
     CDS             complement(572..1051)
                     /locus_tag="LP327_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBL1726255.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="LP327_002156"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="LP327_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="LP327_002157"
     CDS             complement(2098..3204)
                     /locus_tag="LP327_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL1726256.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="LP327_002158"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="LP327_002158"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBL1726257.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="LP327_002159"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="LP327_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBL1726258.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="LP327_002160"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="LP327_002160"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBL1726259.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="LP327_002161"
     CDS             8175..8873
                     /locus_tag="LP327_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL1726260.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="LP327_002162"
     CDS             complement(9041..11680)
                     /locus_tag="LP327_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL1726261.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="LP327_002163"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="LP327_002163"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBL1726262.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..>14118
                     /locus_tag="LP327_002164"
     CDS             13523..>14118
                     /locus_tag="LP327_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1726263.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2543 g         4520 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagc
//
LOCUS       DAFEHS010000070        13807 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFEHS010000070
VERSION     DAFEHS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.072"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LP327_002165"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LP327_002165"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBL1726264.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="LP327_002166"
     CDS             complement(2260..2511)
                     /locus_tag="LP327_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBL1726265.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="LP327_002167"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="LP327_002167"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBL1726266.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="LP327_002168"
     CDS             3461..3679
                     /locus_tag="LP327_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726267.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="LP327_002169"
     CDS             complement(3968..4480)
                     /locus_tag="LP327_002169"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBL1726268.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="LP327_002170"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="LP327_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBL1726269.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="LP327_002171"
     CDS             complement(6933..7376)
                     /locus_tag="LP327_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBL1726270.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="LP327_002172"
     CDS             complement(7377..8165)
                     /locus_tag="LP327_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL1726271.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="LP327_002173"
     CDS             complement(8162..9103)
                     /locus_tag="LP327_002173"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBL1726272.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="LP327_002174"
     CDS             complement(9280..9867)
                     /locus_tag="LP327_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBL1726273.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="LP327_002175"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="LP327_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBL1726274.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="LP327_002176"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="LP327_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL1726275.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="LP327_002177"
     CDS             complement(12784..13719)
                     /locus_tag="LP327_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL1726276.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DAFEHS010000071        13732 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFEHS010000071
VERSION     DAFEHS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.073"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(63..1268)
                     /locus_tag="LP327_002178"
     CDS             complement(63..1268)
                     /locus_tag="LP327_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL1726277.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="LP327_002179"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="LP327_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="LP327_002180"
     CDS             complement(1447..2832)
                     /locus_tag="LP327_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL1726278.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="LP327_002181"
     CDS             complement(2861..3247)
                     /locus_tag="LP327_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL1726279.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="LP327_002182"
     CDS             complement(3263..3577)
                     /locus_tag="LP327_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL1726280.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="LP327_002183"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="LP327_002183"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBL1726281.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="LP327_002184"
     CDS             4837..6126
                     /locus_tag="LP327_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBL1726282.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="LP327_002185"
     CDS             complement(6165..6770)
                     /locus_tag="LP327_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBL1726283.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="LP327_002186"
     CDS             complement(6966..7430)
                     /locus_tag="LP327_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL1726284.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="LP327_002187"
     CDS             complement(7452..7772)
                     /locus_tag="LP327_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL1726285.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="LP327_002188"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="LP327_002188"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBL1726286.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="LP327_002189"
     CDS             9194..9544
                     /locus_tag="LP327_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL1726287.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="LP327_002190"
     CDS             9729..10682
                     /locus_tag="LP327_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL1726288.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="LP327_002191"
     CDS             10695..12482
                     /locus_tag="LP327_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726289.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="LP327_002192"
     CDS             12469..13671
                     /locus_tag="LP327_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726290.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3937 a         2689 c         2520 g         4586 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt gaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg ga
//
LOCUS       DAFEHS010000072        13672 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFEHS010000072
VERSION     DAFEHS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.074"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(260..547)
                     /locus_tag="LP327_002193"
     CDS             complement(260..547)
                     /locus_tag="LP327_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL1726291.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(537..866)
                     /locus_tag="LP327_002194"
     CDS             complement(537..866)
                     /locus_tag="LP327_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL1726292.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            1171..1500
                     /locus_tag="LP327_002195"
     CDS             1171..1500
                     /locus_tag="LP327_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1726293.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(1787..2272)
                     /gene="vanZ"
                     /locus_tag="LP327_002196"
     CDS             complement(1787..2272)
                     /gene="vanZ"
                     /locus_tag="LP327_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL1726294.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2425..3336)
                     /gene="vanY"
                     /locus_tag="LP327_002197"
     CDS             complement(2425..3336)
                     /gene="vanY"
                     /locus_tag="LP327_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBL1726295.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(3764..4372)
                     /locus_tag="LP327_002198"
     CDS             complement(3764..4372)
                     /locus_tag="LP327_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL1726296.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(4378..5409)
                     /gene="vanA"
                     /locus_tag="LP327_002199"
     CDS             complement(4378..5409)
                     /gene="vanA"
                     /locus_tag="LP327_002199"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL1726297.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(5402..6370)
                     /locus_tag="LP327_002200"
     CDS             complement(5402..6370)
                     /locus_tag="LP327_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL1726298.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            complement(6585..7739)
                     /locus_tag="LP327_002201"
     CDS             complement(6585..7739)
                     /locus_tag="LP327_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBL1726299.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(7717..8412)
                     /gene="vanR"
                     /locus_tag="LP327_002202"
     CDS             complement(7717..8412)
                     /gene="vanR"
                     /locus_tag="LP327_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBL1726300.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            complement(8626..9201)
                     /locus_tag="LP327_002203"
     CDS             complement(8626..9201)
                     /locus_tag="LP327_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL1726301.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            9347..12271
                     /locus_tag="LP327_002204"
     CDS             9347..12271
                     /locus_tag="LP327_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBL1726302.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            complement(12356..13216)
                     /locus_tag="LP327_002205"
     CDS             complement(12356..13216)
                     /locus_tag="LP327_002205"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL1726303.1"
                     /translation="MEYLTIEELKHKYPVIHLCDILGIAKSSYYKWLKREPSETELKR
                     LKLMRAIKGIHEAFGGIYGYRRMTIFLNFFRRAKVNHKCVHRLMRIMGITAVIRRKRR
                     NYVPHKAVHVAENILNRDFHAERPMEKLLTDVTEFRLTNGTKRYLSAIYDLGSKKIVA
                     YKTSHRNDNPLVLDTLKQILGDVKPETTLIHSDRGSQYTSHAFNKMIKDHQIIHSMSR
                     VSKCIDNGPMEGFWGTLKVEMFNLDTFDRPAYLDRKIKAYIAFFNNERVTLDMGLAIP
                     TEEKILQMIA"
     gene            complement(13204..13551)
                     /locus_tag="LP327_002206"
     CDS             complement(13204..13551)
                     /locus_tag="LP327_002206"
                     /inference="COORDINATES: protein motif:HMM:NF024908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726304.1"
                     /translation="MTGVKKSYEEKLKIVEDYIANRLTYLEAAEKNHVSYSNIYSWVN
                     KYKKHGPIGLEDNRGRGKPTEIQTTEERLNAEVETLKARNKWLEMENDALKKRRKIAG
                     NHKSQGLDKKWNI"
BASE COUNT         4060 a         2743 c         2450 g         4419 t
ORIGIN      
        1 atctataata tttatgggag aattagagtg atttttttat tattactaaa aaacaatata
       61 aaataacgca ttttaagccc tctttgtgca taaggatgaa gcttttaatt tgaaagaggc
      121 ttaaaatgcg ttttaatttt ttctattttt cataaatata taggttagtg gtttaaaatt
      181 atagggtatt acaactctca gctgttaaaa attatctttt aaagtgtcgt ctatcctctc
      241 aagttgaaac attgggttct tacctaatct tttttaattg ttcataaaaa ttttcatgcg
      301 ttccacatag taagataggt atcactgcat tatctttaat ttcatatgca acgcgatatg
      361 tcgtacctgc atacttgaat ccaatagccc aaattcctga taaatcaccc gttttttgtt
      421 ctcccgaatg aggacgaact gctatctcat cataaattat cgttaaaaat tgttctttta
      481 atgttttctc ttttaatttt tttagatact ttattacgtg tttagaaggt agaatctcat
      541 aagccaattt ctgctgccaa ctcctctttt gtcatcggtt tagaatttgc aacagtatct
      601 tcagcaatag atttaaatgc agaagttaac gcattttttc tatttctaaa ttcttttaag
      661 atctcttgtt tgctataacc ttctgaaaga atatcagcca aaatatcttc actaaaatca
      721 aaaaattctt ttttaggctc tacaaactca taaaggatac cattctctac aagcttagct
      781 tctacttcaa caccattggg cacatcgaat tctttaggga ccgttaacat aatagaattt
      841 ccttgttttc ttgtttttgt aatcatatta tttcctccat tattttacat ttcgtaatta
      901 cttagtaatt acattgtacg ctatttttag tagaaataca aaataataaa actcctttag
      961 aacaattttc aaaggagttt tatttgatta ggaaattatg tagaataata atatgttttt
     1021 aacttggaat caaaaacgca cttgcaagta agtttacttc aatacatgaa attgcttata
     1081 atagtgtatt ggaattaagg tagattgaca aaagagtgat caaataggta tacttcattc
     1141 aaataatcgt ttgaatgtga ggtgagatga atgaaaaaag tttgccaagt gactgtcgta
     1201 cataaagaaa aagtaaatgg aataaaaaat aaattggatc aaaaagattt ttcaaactta
     1261 cttgtgttag ggaaatgttt cagcgatttt tctagaatta aaattttcta tgctttagaa
     1321 acctataagg aaatgtgcgt ctgtgattta gcagaaatac ttactgctag tgtcgctaca
     1381 acgtcacacc atttacgttt tttaaaaaaa catggaatgg caaaatcacg tcaagatgga
     1441 aaagtagttt attactcttt agcgaatgaa gacatccttt ccgctattcg tgttttttaa
     1501 agttatcaga aaatctatct atgaaatctt agtttggggg taccgtcagg aaaatgcgga
     1561 tttacaacgt taaggaaatt tattaaagag accaattcct cagcactaaa attgataatt
     1621 gggctttctt tgttactata gtgttaggta agctttttac tttatcaccg gttttcccct
     1681 cacttcacac ctacgggcga gtttccccac atcccgtttt ccatctaatc caattcattc
     1741 aatatcttta tgtgtatgaa agatcagatt gcttgattta gacatcttac acgtaattta
     1801 ttcttaaatg ggtacggtaa acgagcaata atacgagcaa aagtatacct acaaaaataa
     1861 taactctgtc taattttttt tgattcatat gcttattgct taaaccatat aatttcagtc
     1921 caagaaagcc tccaacagta tttgtaatta catctgttat gtctgtcgct ccaatagcga
     1981 agataaattg aattatttca aaagtaagac ttaaaaccag tacaaaagca aacttaggta
     2041 aaaatccgat ttctttaaaa ttgacattca aaagcaagcc aaatggaata aagattataa
     2101 cattatctat catctctctg aaattcccag tagcagtaaa tggagtcaag ttaagacttc
     2161 tttgatgata attaaatact gataaaatat tgtattgtaa tttgaataac actaaccaga
     2221 ttagtgtcac taaatataaa gctagcaatc ctctagataa tatttttccc aatataattt
     2281 cctctaattc ttttgtttaa gttgtatcat tagtatagca atgtcctagt taacgtgggt
     2341 tttaaattaa aaagctaacg ttaccccaaa agtgctagta caataatgct gttcattaat
     2401 ttcgttggtt tcattccgcc atccttacct ccttgaatta gtatgtgttg atccgggaaa
     2461 cactgtcaca attacaccgt ctatattgtt tcctgatatc tcataacgaa gattagtcgg
     2521 cacatgaatg gtggtatttt tagtaacagg ataataaaag atctcatatt tttccccatt
     2581 tacactaaca gaaatggttt tttcttcttt taggtaatcc atatattcct cgagaacgaa
     2641 attcttttct ttcataatcg cactatgtgg taaaccaaca tagcgaatat gccatagttc
     2701 atattgaatt cctgttaact ctgttttgtc ctctggataa cgtaaaatga acccgtattt
     2761 ccaagcattt tcttctatcc actttccttc aggggctcgt tccattttcg tcaagcttga
     2821 tcctacatct agtgataaac ctgaattatg ctcactataa cctgctggta aggcatactc
     2881 agcccccatt tcttggtaaa gcacactttg ctcatcaaag tctcgatagc cactattaat
     2941 aataaaatga ctaacgccac cctttacagc atcattgacc atctctgaaa atttttgtgc
     3001 tatttctttt gacatataaa tattactatc aagcaacccg tatccattta ttaattcgtc
     3061 atgtttagat aaattcacga tatctgactt cacactttct tggcgaacag gatatttact
     3121 attgattaat agcagatttc cttgataaac ctgttcttct gtaattgttt tctcttgggt
     3181 attttcagaa gtcccactat tttctaaatg ttctttggga ttttgatcat aattttgaaa
     3241 ttcgactttt tcctgagaaa acagtgcttc attaacgtag tcataaccta agtatattaa
     3301 gaataacaat aacaataaaa aaaacaactt cttcattttc agtctcctcc ttaactatat
     3361 agttggaagt atgaaatatt tctagaatct tccccttaaa cggcacaatc tcgtttgaga
     3421 ttgtgccgtt ttgccccgta aattacagtc aattatttcg ccctactgga tttactatta
     3481 tcaccaatgt agatgaattt tcataagtga tacccatgct gaatttaacg aggctgaact
     3541 gcctgttgac aatgggcggt gtgaaatacc acccctctat gcgatcagtc ttattatcag
     3601 gctatcttgc cgcagcgcac gcagataatg accgcaatat cgctctgcct tacatcttca
     3661 atactatcta ctatatataa attccctaag aaccagttac atacgtcggg tttcctgccg
     3721 aaagagttag cttatatagt ttgtccgtgc aacggttaaa agtttattta acggggaaat
     3781 caaaatagct attggggtat ggttcgtctc ttaatacata gtgccaccat tcgaggctat
     3841 atgcttcaaa cccactgttt tccatgatgg agcgcaaacg tctgcgattt tgcgcttcat
     3901 tgcatgatat tccatttgcc gcatgatgag agcgttcatc cataaaatca aatcggctcc
     3961 ccattggtac aagctcaccc gtgtctaatc gataaagcgt aagatcaatg gcactgccgc
     4021 ggctatggct tgattttgaa gccacgtatc cttttgaaat catctcagtt cggtcaatat
     4081 tgggataata actttccttt gtcaggttat tttccggctg tgcagcccat tgcataaaac
     4141 agtttacagc acgattagga cggtaaccgt cccatagaag caatccgtac ccttgggtag
     4201 cagccagttc ttttgccttc aaaagcgatt cagccaactc gtatgtccct acaatgcgat
     4261 ttacttcata accgtcaacc ggttttccgg tgaaattatc ccaagtggca tatttagcgt
     4321 cccaacgaac accgtgtact atttcatcta aaaaagtaaa tcctatttcc atgcttatca
     4381 cccctttaac gctaatacga tcaagcggtc aatcagttcg ggaagtgcaa tacctgcagc
     4441 ggccatcata cggggataac gactgtatga cgtgaaaccg ggcagagtat tgacttcgtt
     4501 cagtacaatg cggccgttat cttgtaaaaa catatccaca cgggctagac ctctacagcc
     4561 gagcgcttta tatatttttt ttgccgtttc ctgtatccgt cctcgctcct ctgctgaaag
     4621 gtctgcggga acggttataa ctgcgttttc agagcctttt tccggctcga cttcctgatg
     4681 aatacgaaag attccgtact gcagcctgat ttggtccacc tcgccaacag ctaacgcggc
     4741 actgtttccc aataccgcac aaccgacctc acagcccgaa acagcctgct caattaagat
     4801 tttgctgtca tattgtcttg ccgattcaat tgcgtagtcc aattcgtccg cgctattgac
     4861 ttttttcaca ccgaaggatg agcctgaacg cgccggctta acaaaaacag gataggtaaa
     4921 cgtagctgcc accggcctat catctttatt aataacccaa aaggcgggag tagctatccc
     4981 agcatttttc gcaacgatgt atgtcaacga tttgtccata caaattgctg agctttgaat
     5041 atcgcagcct acaaaaggga taccggacaa ttcaaacaga ccttgtatgg atccatcttc
     5101 acctgacttg ccatgcaaag ctgaaaatgc tacatcaaca tggttgattt catattcatg
     5161 gttcttttta acaagtaatc cgtgcatttt tttatccggc gagagtacag ctgaatagca
     5221 attgtcgttt tcccattccg cgcaaggttt ttcgcacatt ttccatacac cagatttcgt
     5281 aattccaatg tataacggct cgtatttttc tttattaatg ttagcggcta tctctattgc
     5341 agattttacc gatacgtcat gctcctctga gcaaccccca aacagtattg caacttttat
     5401 tctattcatg ctcctgtctc ctttcaaaat ccaaacagtt tttaatggtt ttttcaacgg
     5461 tatcacgcaa cgcttgctcg gtataatagg ccgtatgcgg tgtgattatc acgttaggca
     5521 ttctttgaag tttaagtaaa aattgattat caattggttt ttgggtgcaa tcagagtaga
     5581 aaaactcttc ctctccttcc aatacatcca atgcggcacc gcccagtttc ccgttttcta
     5641 atgctttaac caactcatag gtatctacaa gtggaccgcg cccagtattg ataagaaatg
     5701 ctccttgctt cattctctgt atttgttcgt ggctgataat atagtgcgta tccgtattga
     5761 gcggcacatg aagcgtaacg atatcgctat tttgcagcaa ctcatcaaac ggtacatagt
     5821 ttacctctat acttcggctg cgactataag ccaacacttt acatccaaat cctcgcagcc
     5881 gctcaataac cgctttgcct atctggcccg ttcccaccac accaactgtc atgtcgctga
     5941 gtaccttgcc acggtcgctg tccaacctga aatcatgttt ttccacagag cgcacaatcg
     6001 attttacgtt gcgtactgcc ataagaatta gcatcatagt ataatcggca acgctatccg
     6061 gcgagtacgc cacattgtcg acagtgatgc ccattctctt agcagcagtt gtatctatat
     6121 gattgcagcc gatgcttcgg gtagaaatat atttcacacc ggctctcttc agcgcaagaa
     6181 gaatagaggc ggaaatctct gatttatgtc ccacactgat acattgattg aaaggcgcgg
     6241 atttggcgtt ggattccgac acgttggcgt taattatcgt tgccataacg ccaaagcgag
     6301 gcgaaagagc atggaatgca tctgcctcat cctgctcaca tccataaaca gtaatgccga
     6361 tgttattcat aattaagacc aaccctttct gtgaaaggca ccaaataaaa cggggatgcc
     6421 aatggtaatc ttgtcaaatc tttctctgaa gaacgaaaac ggctcgttct tacggtatta
     6481 agtctaccgt gtaagaacga gccgtttctt aatattaaat tgttaatttc ctaagaaaaa
     6541 gtaaagataa ccttgagatt ttcctaaaaa attatataca tctcttagga cctcctttta
     6601 tcaaccaagt ctggcatcgc tggaagctct accctaaacg tcgtatagtt atcattgctt
     6661 tccgcgtaaa tctgccctcc atgctgaaca ataatttctt ttgcaatcgc caatccaagt
     6721 cccgcgccac ccgtatcgga agaacgagca ttgtccagcc tatagaactt ttcaaatatg
     6781 gcagctagct tatcttttgg gatgcttcca gtgttcttga attcgattga caccacatcc
     6841 ccggagaggc ccgcggtaat gtcaatgatg ctgttatcct cactgtatgc agcggcgttt
     6901 ttcaaaatgt tgttaaagac tctcgcgagt ttatcagggt cgccggacac ggtcagatcc
     6961 tcgggggcgt gaataaccgc ctgttttcca tgtgcggaaa gctgaggata aaattcatcg
     7021 gtcatctgca ccagcatata gtataggtct atgtgcgttt ttgttagcgt tatcgtttgt
     7081 aggttatacc gtgtaatctc aaaaaactcg tcgattagct gttcgagtcg atacgctttg
     7141 tccaacgtga tatgcacata ctttgccttt tgatctaccg gcatgtctgg agcctcgtca
     7201 agcaggctca aataaccgat aatggatgta aggggcgttt taatatcgtg cgccaagtac
     7261 ataacaacgt cattttttct ttgttcggcc agctttgcat cctgctctcg cttttccaga
     7321 gtccgtttta atgtgttgag cttttgttcc ataacatcca tttccgcaga aagctcaatt
     7381 tgtttatctt cgttctgaat aagtacatca atgccggtat ttatctcgtc aaagtatttt
     7441 gcgaattttg aaagcatgac gcgacataga ataagaatac taatgacaat cgccacataa
     7501 ataaagatat ctatattgtt ccgtatggaa tattgatata atttcatcgc gtccaggtga
     7561 tttaagtcat atttgttttc caaaatactt aagatccaat ccccaagttt ccctcggatc
     7621 attgaacgaa tatacaacac gaatacaatt gctaccacaa caattgcaac gatatacatg
     7681 taaagttttc gttctagttt ggaatagtcg ttttttttat ttttcaattt tataaccaac
     7741 cccccatacc gtttttatat atttcggatt atcaatggtg tcgttcattt tttcgcgcaa
     7801 atgccggata tgcacggtga tggtgttgtt gctcttgctg aaatattcgt cgccccatat
     7861 ctcatgaaat agcagctcgg agctaaccac attccccttg ttttcacaga ggattcgcag
     7921 tattgaaaac tcggtgggag taagggataa ctgcttctcg ttcagataac actcatgggt
     7981 gttaacatta atgacaaggc cggagtggac gataacattt tcgttctgct cctttactcc
     8041 actgaatttt ttgtatcggc gcaactgggc ctttacccga gcaattaact ccagtgggcg
     8101 aaagggcttc gttatataat catccgcgcc gattgttaac cctgtaattt tatctacctc
     8161 tgtatctttc ccggtcagca tgataatcgg ataggtgtgc ttgtccctta ttttttgaca
     8221 gatagtaagg ccgcttgtgc cgggaagcat gatgtccaat atggcaaggt caatctcaga
     8281 cttgtctata cattccaatg cttctttggc ggtatagtat ttgaaaaccg tataattctc
     8341 gttttttaag tataattcaa ccaaatcggc aatttcatgt tcatcatcca caataagtat
     8401 tttatcgctc atagttatca ccctttcaca taagtttgcc cttaataatt tatcagatta
     8461 tagggccgaa tttataagca tttacataat aaatgactaa gaatttctta agatgatgat
     8521 tcttttttta tcaaagggct tttcggcatc gctaggaggc tgttaatatt ttatagtttc
     8581 cttttcttct ttaaaaatat tgcccattag cggaatacag aatggctaat tattcacttc
     8641 tgataatttc ctgtataatg aagccctaga tacattagta atttcacaaa tttgatttac
     8701 agtcatattt ccttctttat atagctttac cgcataattc attcctgcgt gatttttatg
     8761 atacttcttt aatcgacctt taaactttcc ttctttctta gccaattcaa tcccttcacg
     8821 ttgtctcatc cgaataagat ctcgctctaa ttggttaaca ccagccatta cagtaattaa
     8881 gaattggctg tatggattat cttctgataa atcaagccat gtatctttta gtgattttaa
     8941 acttgccttt ttatctcgta tgttatcgat taattcaaat agatcttgtg tactacgagt
     9001 gattcgagtt aagtctgtaa cataaatgat gtcatcttcc tgtaaatcgt ctaacacttt
     9061 ttgaagttgc tcgcgatcct ttgttgctcc tgaaactttc tcttcatata taatatccat
     9121 tccgatctcg ttcaactgct gaaattgtct tgaaggattc tggttagtcg aactgacacg
     9181 aatataaccg attttccgca aaatatcacc tcatttttga gacaagtctt atgagacgct
     9241 cttaactatg attttatcag tctactacat ttgtatcaat agagtacact ctattgatat
     9301 ataattgaac taataaattg aaaatacaga aatggaatga tactgaatga aaattgcgag
     9361 aggtagagaa ttgcttacac cggaacagag acaggctttt atgcaaatcc ctgaagatga
     9421 atggatactg gggacctact tcactttttc caaacgggat ttagaaatag ttaataagcg
     9481 aaggagggaa gaaaaccgtt taggatttgc tgttcaatta gctgttcttc ggtatcccgg
     9541 ttggccatac actcatatca aaagcatccc agattcggtc atacaatata tatcgaaaca
     9601 gattggtgtt agtccatcct cgcttgatca ttatcctcaa agggaaaata cactttggga
     9661 tcatttgaaa gaaattcgaa gtgaatacga ctttgtaact tttaccctga gtgaatatcg
     9721 aatgacattt aagtaccttc atcaattagc tttggaaaat ggtgatgcca ttcatctact
     9781 gcatgaatgc atagattttc taagaaaaaa caaaattata ctgcctgcta tcactacact
     9841 tgaaagaatg gtgtgggaag caagggcaat ggctgaaaag aagctattta atacggttag
     9901 taaatctcta acaaatgagc aaaaagaaaa gcttgaaggg attattacct cgcagcatcc
     9961 atccgaatcc aataaaacga tattgggttg gttaaaagag ccaccgggtc atccttcacc
    10021 cgaaactttt ctaaaaataa tagaacgact cgaatacata cgaggaatgg atttagaaac
    10081 agtgcaaatt agtcatttgc accgtaaccg cctgttgcag ctgtctcgct taggctcaag
    10141 atacgagccg tatgcattcc gtgactttca agaaaataaa cgttattcga tattaaccat
    10201 ctatttatta caacttactc aggagctaac ggataaagcg tttgaaattc atgataggca
    10261 aatacttagt ttgttatcaa aaggtcgtaa ggctcaagag gaaatccaga aacaaaacgg
    10321 taaaaagcta aatgagaaag ttatacactt tacgaacatc ggacaagcat taattaaagc
    10381 aagagaggaa aaattagacg tttttaaggt tttagaatcg gttattgaat ggaatacctt
    10441 tgtctcttca gtagaagagg ctcaggaact tgcacgtcct gccgactatg attatttaga
    10501 cttactgcaa aaacggtttt attcactaag aaaatatacg ccaacgctat taagagtatt
    10561 ggaatttcat tctacaaagg caaatgagcc acttttacaa gctgttgaga ttatccgagg
    10621 aatgaacgaa tctggaaagc gaaaagtgcc tgatgactca cctgtggatt ttatttcaaa
    10681 acgatggaaa agacatttat acgaggatga tggtacaaca attaatcgtc attactatga
    10741 aatggctgtt ttaacagaac ttcgggagca tgttcgggca ggagatgttt ccattgttgg
    10801 cagcagacaa tatagggatt ttgaggaata tttgttttcg gaagatacat ggaatcaatc
    10861 gaaggggaat acgagattat cagttagttt atcattcgaa gattatataa cggagagaac
    10921 cagcagcttt aatgaaaggt taaagtggtt agctgccaat tccaataagt tagatggggt
    10981 ttctcttgaa aaaggaaagc tatcacttgc acgcttagaa aaagatgttc cagaagaagc
    11041 aaaaaaattt agtgcaagcc tttatcagat gctaccaaga ataaaattaa ctgatttact
    11101 catggatgtg gcccatataa caggatttca tgagcaattc actcatgctt ccaataatcg
    11161 aaaaccagat aaggaagaaa caatcattat catggctgcc cttttaggaa tgggaatgaa
    11221 tattggcttg agcaagatgg ccgaagccac acccggactt acatataagc aactagccaa
    11281 tgtatctcaa tggcgcatgt atgaagatgc catgaataaa gcccaagcca tattagtaaa
    11341 ctttcatcat aaattacaat tgcctttcta ttggggcgac ggtacaacat cttcgtcaga
    11401 tggtatgaga atgcagctag gtgtttcatc actacatgca gatgcaaatc cacattatgg
    11461 aactggaaaa ggagccacca tctaccgatt tacaagtgat caattctctt cttactacac
    11521 aaagattatt catactaatt caagagatgc gattcatgtt ttggatggtt tgttacatca
    11581 tgagacggat ctaaacatag aggaacatta tacagacact gccggttaca ctgaccaaat
    11641 attcggactg actcatttat taggatttaa atttgcccca agaataaggg atttatcgga
    11701 ctcaaaatta tttacgatag ataaagcaag tgagtatcca aaactagaag ccattttacg
    11761 tggacaaata aatacaaagg tcattaaaga aaattatgag gatgttttgc gattagctca
    11821 ttctataagg gagggaacag tttcagcatc ccttattatg gggaagctag gttcctattc
    11881 aagacaaaac agcttagcta cagccttacg tgagatgggc cgaatagaaa aaacgatctt
    11941 tattttgaat tatatatcgg atgaatcatt aagaagaaaa atacaaagag gattgaataa
    12001 aggagaagcc atgaatggat tggcaagagc tattttcttc ggaaaacaag gtgagcttag
    12061 agaacgcacc atacagcatc aattgcaaag agccagtgct ttaaacataa ttatcaatgc
    12121 tataagtatt tggaatactc tccacctaac aacagcagtt gaatataaaa aacggacagg
    12181 tagctttaat gaagatttgt tacaccatat gtcgccctta ggttgggaac atattaattt
    12241 actaggagaa taccatttta actcagagtg aactgccccc tgtcaagtag acaggtaaaa
    12301 aaacaaagta ttttagacca tgcatgtaaa aatgtgtggt ctttttttgt agcgtttatg
    12361 caatcatttg tagaatcttc tcttccgtag gaattgctaa tcccatatcc aaagtaacgc
    12421 gttcattgtt gaagaacgcg atgtatgcct tgattttccg gtctaagtac gctggtctgt
    12481 caaacgtatc caggttaaac atctccacct tgagggtacc ccaaaagcct tccatagggc
    12541 cgttgtcaat acatttcgag acgcgtgaca tactatggat tatctggtga tctttaatca
    12601 tcttgttaaa ggcatgggag gtgtactggg aaccgcggtc gctatgaatc agtgtggttt
    12661 caggctttac atcacccaaa atctgcttta gtgtatccag tactaacggg ttgtcattgc
    12721 ggtgactggt tttataagcc acgattttct ttgacccgag gtcataaata gcgctcaggt
    12781 aacgttttgt cccattggtc aaccggaatt ccgtgacatc cgtcaataac ttttccatgg
    12841 gtctttcagc gtggaaatca cggtttaaga tgttttcagc cacatgtacg gctttgtgtg
    12901 gcacgtagtt ccttcttttg cgacggatga cggctgtgat ccccatgatt ctcatgaggc
    12961 ggtgtacaca cttatgattc actttcgctc ttctaaaaaa gttgagaaag atggtcattc
    13021 ttcggtagcc gtaaatccca ccgaatgcct cgtggattcc tttgatcgcc cgcatcagtt
    13081 tcaggcgttt taattctgtt tctgagggtt cccgttttaa ccacttatag tagctggatt
    13141 tggcgatacc cagtatgtca caaagatgga taacgggata tttatgcttt aattcttcaa
    13201 tagttaaata ttccacttct tgtctaaccc ctgtgatttg tgattcccag cgatttttcg
    13261 gcgctttttt aacgcatcgt tctccatttc tagccatttg ttccgggctt tgagtgtctc
    13321 tacttctgcg tttaaacgtt cttccgttgt ttgaatttcc gtgggcttcc cgcggccccg
    13381 gttgtcttcc agacctatcg gtccatgctt tttgtattta ttcacccagg aataaatgtt
    13441 actgtaactt acatggtttt tctccgctgc ttccagataa gtcaggcgat tggctatgta
    13501 atcttctacg atttttaatt tttcttcata tgatttttta acacccgtca tgttgtacac
    13561 ctcagatttc ggagaatatg tctcattctc ttttccttca gtatatcgaa ttacccattt
    13621 gtttaccgtt gttttagatg gaatgttata tttagcggct aagtatgaaa aa
//
LOCUS       DAFEHS010000073        13488 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFEHS010000073
VERSION     DAFEHS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.075"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(164..388)
                     /locus_tag="LP327_002207"
     CDS             complement(164..388)
                     /locus_tag="LP327_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBL1726305.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="LP327_002208"
     CDS             complement(586..2166)
                     /locus_tag="LP327_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBL1726306.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="LP327_002209"
     CDS             complement(2567..3943)
                     /locus_tag="LP327_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL1726307.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="LP327_002210"
     CDS             complement(4071..5189)
                     /locus_tag="LP327_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL1726308.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="LP327_002211"
     CDS             5331..5765
                     /locus_tag="LP327_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1726309.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="LP327_002212"
     CDS             complement(5843..6376)
                     /locus_tag="LP327_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBL1726310.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LP327_002213"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LP327_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBL1726311.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LP327_002214"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LP327_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBL1726312.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="LP327_002215"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="LP327_002215"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL1726313.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="LP327_002216"
     CDS             complement(10101..10679)
                     /locus_tag="LP327_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBL1726314.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="LP327_002217"
     CDS             11001..11471
                     /locus_tag="LP327_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBL1726315.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="LP327_002218"
     CDS             complement(11579..12805)
                     /locus_tag="LP327_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL1726316.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3959 a         2719 c         2248 g         4562 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaa
//
LOCUS       DAFEHS010000074        13427 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFEHS010000074
VERSION     DAFEHS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.076"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..912)
                     /locus_tag="LP327_002219"
     CDS             complement(<1..912)
                     /locus_tag="LP327_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBL1726317.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="LP327_002220"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="LP327_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBL1726318.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2004..2174
                     /locus_tag="LP327_002221"
     CDS             2004..2174
                     /locus_tag="LP327_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726319.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2389..3648
                     /gene="lysA"
                     /locus_tag="LP327_002222"
     CDS             2389..3648
                     /gene="lysA"
                     /locus_tag="LP327_002222"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBL1726320.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3635..4435
                     /gene="dapF"
                     /locus_tag="LP327_002223"
     CDS             3635..4435
                     /gene="dapF"
                     /locus_tag="LP327_002223"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBL1726321.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4438..5319
                     /locus_tag="LP327_002224"
     CDS             4438..5319
                     /locus_tag="LP327_002224"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBL1726322.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5316..6032
                     /locus_tag="LP327_002225"
     CDS             5316..6032
                     /locus_tag="LP327_002225"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBL1726323.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6038..6733
                     /gene="dapD"
                     /locus_tag="LP327_002226"
     CDS             6038..6733
                     /gene="dapD"
                     /locus_tag="LP327_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBL1726324.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQR
                     KENTALIAALREL"
     gene            6749..7750
                     /locus_tag="LP327_002227"
     CDS             6749..7750
                     /locus_tag="LP327_002227"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBL1726325.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7747..9108
                     /locus_tag="LP327_002228"
     CDS             7747..9108
                     /locus_tag="LP327_002228"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBL1726326.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9068..10219
                     /locus_tag="LP327_002229"
     CDS             9068..10219
                     /locus_tag="LP327_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBL1726327.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10194..11363
                     /locus_tag="LP327_002230"
     CDS             10194..11363
                     /locus_tag="LP327_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL1726328.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11538..12917
                     /locus_tag="LP327_002231"
     CDS             11538..12917
                     /locus_tag="LP327_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL1726329.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13198..13427
                     /locus_tag="LP327_002232"
                     /pseudo
     CDS             13198..13427
                     /locus_tag="LP327_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2923 g         3681 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tggttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atatgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 attcttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaaac cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatgattca taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat
     2161 tttttcatca ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt
     2221 cgggtatttt gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat
     2281 actagacaat tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa
     2341 atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg
     2401 ataattggcg gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg
     2461 tatgatgaag aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa
     2521 cgttttgata caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat
     2581 ttagttcgtg aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct
     2641 ttgcaagctg attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag
     2701 gaactgatct atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa
     2761 gttgaattat tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg
     2821 ttgaatgtcg gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca
     2881 aaattcggca tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa
     2941 agcagctatc tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt
     3001 actgcttggt tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct
     3061 ctttccttga atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc
     3121 ccagtcgaag aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga
     3181 aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt
     3241 acaacactat acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta
     3301 gatggcggga tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat
     3361 ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt
     3421 gaatcaggcg atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt
     3481 ttagctgttt attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg
     3541 acacgaccag cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa
     3601 gcattttctg atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg
     3661 cggaaatgat tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc
     3721 ttctttatgc acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc
     3781 gttggaaatg atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat
     3841 tcgctgtttt gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt
     3901 agagacatta gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag
     3961 cgtagatatg ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc
     4021 aataaaaaac caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt
     4081 aggaacgatt cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag
     4141 aggtgaactg atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga
     4201 agtagtcaat gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact
     4261 tgcttgcggt acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc
     4321 agcagatcag gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc
     4381 gatacaaatg gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg
     4441 ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat
     4501 gctggtttgg aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt
     4561 ttaggtacaa caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa
     4621 ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat
     4681 acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg
     4741 gtcatcaccc cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca
     4801 attgcagaag cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg
     4861 tcgattccaa tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa
     4921 gaagcctcag gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc
     4981 ttcctttaca gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt
     5041 gtaataagtg tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg
     5101 caggacggaa gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat
     5161 gccttgttca gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta
     5221 ccttccggac ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta
     5281 gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg
     5341 gacagatggg aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt
     5401 tacggacggc acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta
     5461 ttgatttttc tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac
     5521 ttccgttagt cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac
     5581 atgccaaaca tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga
     5641 atcaactaat caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa
     5701 aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa
     5761 tccaagaagt gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa
     5821 accaaattgg tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt
     5881 ttgctgctac tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg
     5941 ctgcaggcgc agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat
     6001 tagaagattt attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg
     6061 ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc
     6121 aatcgaattt aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga
     6181 ctatgagacg attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat
     6241 cgaaactgct gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg
     6301 tatcgaacca ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat
     6361 gatgggagcg attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg
     6421 tgctgtctta ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt
     6481 cttagcaggt gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact
     6541 ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc
     6601 agcaggagca gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc
     6661 acgtgtatta aaaataagtg atgatcagag aaaagaaaat acagcattga ttgctgcgtt
     6721 acgcgaatta tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc
     6781 aaccggatta gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg
     6841 acgattgaaa ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa
     6901 gaaacacact gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt
     6961 ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt
     7021 tgtcatagat aattcctcag catggcggga agattcggat attccattaa tcgttccaga
     7081 ggtcaatctg gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat
     7141 tcaagcggta cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta
     7201 ttcgacctat caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac
     7261 gcaaaatgac gaggaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga
     7321 aatcgatcag cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac
     7381 ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat
     7441 cgaaaatgga catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga
     7501 aattagagag tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca
     7561 taaatatccg acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag
     7621 aaaagacact tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa
     7681 aggagccgct gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt
     7741 ggcaagatga gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa
     7801 gagcaattca aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt
     7861 gtttctgctt taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg
     7921 atttatgctt accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat
     7981 cgtttcattc aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca
     8041 cgcatacaga aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt
     8101 gaatatctga ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa
     8161 gacctgattt tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc
     8221 acaaaagcat ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct
     8281 gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg
     8341 aagatgttgc aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct
     8401 gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga
     8461 gagatggctt atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag
     8521 gctgatatcc ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata
     8581 tcaaatcacg aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt
     8641 ctttccatca ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca
     8701 atgagcattt ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat
     8761 atcggagtga tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa
     8821 ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct
     8881 gtcgttgggg gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg
     8941 aacaaattag aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc
     9001 atcggtgtac cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg
     9061 aatacggatg ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca
     9121 tgaattcaag acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca
     9181 tgaaatcggc gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc
     9241 ttttcgaaca gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc
     9301 taagcatgaa ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg
     9361 gttgactgac tttataacta aacacattga ggatcttatt tataatgtag tactgatcta
     9421 tcagccatca gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga
     9481 taaatatgaa gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga
     9541 agttttctct cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa
     9601 aggccagtct gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt
     9661 acgatttttg aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct
     9721 aatgaaattt ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat
     9781 tgtatccgga agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt
     9841 agaccattta gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg
     9901 ctatccagct gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact
     9961 acatgaatta gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata
    10021 tccatgtgta tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt
    10081 cgattttgat atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac
    10141 agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga
    10201 atttatccaa tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat
    10261 tcgatcagca aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt
    10321 ttccaactcc tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc
    10381 actataccgg aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac
    10441 agcgttatgg attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg
    10501 aagcgattgc ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag
    10561 cgcctgctta ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga
    10621 tagatacttc tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac
    10681 aatatggatc agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga
    10741 cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg
    10801 ttataagtga tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta
    10861 cttatcttcc agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg
    10921 gttggcgagt gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc
    10981 atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa
    11041 taaatggggt gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc
    11101 tggtccagca attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact
    11161 tgttttgcaa actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag
    11221 cggaacaggc aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt
    11281 atatccggat ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca
    11341 aagacttttt aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg
    11401 cggtatattc tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt
    11461 ttgattcata actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat
    11521 tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag
    11581 ggcatcaatc gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat
    11641 ttgaagcagg agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca
    11701 aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg
    11761 ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg
    11821 ttactagcgc atcagataac gaaacaaaag aatatcctta tgacaaattg attttgagtt
    11881 ctggtgtcaa accaaacaat ctgccagtac caggagcaga tctagaaaat gtctacttga
    11941 tgcgtggata caattgggca accaaaatca aagaaaaatt aaatgatccg gctattaaaa
    12001 aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc agcagaagca agccgaaaag
    12061 ctgggaaaga agtcgtattg cttgatgtga tcgatagacc tttaggcact tatttagatg
    12121 ctgagatgac agatatttta gaacaacacc taaatgaaaa cggtattgaa gttttgacaa
    12181 atgcaaaaat cgaagctttt actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa
    12241 aagaaatccc agcagatcta gtcattcaag ctgccggtgt gaaggcaaat acagaatggt
    12301 taaaagggat cgtcgactta gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa
    12361 cgaatcttcc agatgtgtat gcagtaggag atgctacact tgcttattct atcccagcac
    12421 gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa
    12481 aacatctttt tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat
    12541 taagtgtctt tgactatcat tttgcagcaa gcggattgaa tagctttaca gcagcaaaag
    12601 caggagtaga tgtacgagtg tcttattatg aagatacgtt acgaccaaaa tatgtaccag
    12661 aagaacaagg aaatccaaaa gtgtccatcc aattattctc ggatccaatg acccatcaat
    12721 tgctaggagg agcagtatta tctacctatg atgttacagg tcaaggaaat gtattggcac
    12781 tagcgatcca aaacaaactg actttagaag aactagcaga agctgacttc ttcttccagc
    12841 caggctttga ccgacaatgg agcatgttga atctagccgc tcaacaagcg ttaggagaag
    12901 aaccatttgt agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa agctgccttg
    12961 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggctggac
    13021 ttattaccgc agatgcaagc ttttgaacac cgtttatttt caaaaaaggg actgtgacac
    13081 gatttgtgta ccgaccccca aaagttagac caaaaaatct aacttttgga ggtcggtatt
    13141 ttttatggtt aaatatactt ttgaacttaa attaaagatc gttcatgatt atttggatgg
    13201 caaaggcgat tggaccgtaa aaatccagat caagagattg aagcggaaat gcttaaaatt
    13261 cgggagaagc acaaggatta tggctgttta cggatgacca atgaactaag aaaccgcggt
    13321 ttctctatca ataaaaagaa agttcagcgc ttgatcaaga agctaggaat aaaagtaacg
    13381 gcatacacgc gtaaatcacg tcgctacaat tcttatcgag gaaaagt
//
LOCUS       DAFEHS010000075        13380 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFEHS010000075
VERSION     DAFEHS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.077"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(45..1364)
                     /locus_tag="LP327_002233"
     CDS             complement(45..1364)
                     /locus_tag="LP327_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL1726330.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="LP327_002234"
     CDS             complement(1361..2014)
                     /locus_tag="LP327_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1726331.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="LP327_002235"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="LP327_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="LP327_002236"
     CDS             complement(2724..3440)
                     /locus_tag="LP327_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBL1726332.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="LP327_002237"
     CDS             complement(3564..3749)
                     /locus_tag="LP327_002237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726333.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="LP327_002238"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="LP327_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="LP327_002239"
     CDS             complement(5356..6387)
                     /locus_tag="LP327_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726334.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="LP327_002240"
                     /pseudo
     CDS             complement(6394..7224)
                     /locus_tag="LP327_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(7221..8036)
                     /locus_tag="LP327_002241"
     CDS             complement(7221..8036)
                     /locus_tag="LP327_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL1726335.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="LP327_002242"
     CDS             complement(8033..8857)
                     /locus_tag="LP327_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBL1726336.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="LP327_002243"
     CDS             complement(8847..9947)
                     /locus_tag="LP327_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL1726337.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="LP327_002244"
     CDS             10125..10910
                     /locus_tag="LP327_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBL1726338.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="LP327_002245"
     CDS             10943..11326
                     /locus_tag="LP327_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL1726339.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="LP327_002246"
     CDS             11402..11533
                     /locus_tag="LP327_002246"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL1726340.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="LP327_002247"
     CDS             11502..11738
                     /locus_tag="LP327_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL1726341.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="LP327_002248"
     CDS             complement(11816..12787)
                     /locus_tag="LP327_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBL1726342.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4066 a         2918 c         2312 g         4084 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcattta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
//
LOCUS       DAFEHS010000076        11721 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFEHS010000076
VERSION     DAFEHS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.079"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(251..1462)
                     /locus_tag="LP327_002249"
     CDS             complement(251..1462)
                     /locus_tag="LP327_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL1726343.1"
                     /translation="MSEVTGYLEQVDNEKYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEVRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1571..2302)
                     /locus_tag="LP327_002250"
     CDS             complement(1571..2302)
                     /locus_tag="LP327_002250"
                     /inference="COORDINATES: protein motif:HMM:NF021378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBL1726344.1"
                     /translation="MGFWDSVKSGAKDIARQSSLENMVQNNKELSSDELEEFVGFTKM
                     TPDEAIAKKPARYFKSTNRYGEVEIDSVKKLFRVKLNVYTFDELNSYELLENGSSVMS
                     GGLGIGRAVVGGVLAGGVGAVLGGVTKKKKQRNYVDSLKIMVTFKNRTPQSVTLDYIK
                     KKQEKDKKYEKTLMKAKETMAGFDYIVSELEVDRHDELVSSTNVQKEQPTTSADEIRK
                     FKELLDEGIISQEEFDAKKKELLNL"
     gene            complement(2394..3059)
                     /locus_tag="LP327_002251"
     CDS             complement(2394..3059)
                     /locus_tag="LP327_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726345.1"
                     /translation="MSERNLKQVDILNMSLPLQKETGIKMSKSHLSQYVNGKSSPDQH
                     KLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQTKVYNFAEYQLRE
                     QNKRPKTTIEIRGYVSAGTGEWLEDEIVDEVSYEGVIPEHDFAVKVNGDSMLPLFEDG
                     QVIFIKSTSDVRDGQIIVCQVNNEAFVKKLSGNKLVSLNKKYEDISICDTDDFKIYGV
                     VVL"
     gene            3255..3479
                     /locus_tag="LP327_002252"
     CDS             3255..3479
                     /locus_tag="LP327_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HBL1726346.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            3503..4264
                     /locus_tag="LP327_002253"
     CDS             3503..4264
                     /locus_tag="LP327_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBL1726347.1"
                     /translation="MKTQKFESNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNALADKFQDWLAVEVLPQIRK
                     HGMYATDDLLNNPDLLIEVATKLKEERTLRLVAEQKAAEMQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            4276..4533
                     /locus_tag="LP327_002254"
     CDS             4276..4533
                     /locus_tag="LP327_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726348.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKMF
                     LKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4504..4815)
                     /locus_tag="LP327_002255"
     CDS             complement(4504..4815)
                     /locus_tag="LP327_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726349.1"
                     /translation="MSRKTNVKISGDLEKIKIFADTLVVGYDDASNIEEKDGMFYSSQ
                     IYVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGF
                     K"
     gene            4866..5045
                     /locus_tag="LP327_002256"
     CDS             4866..5045
                     /locus_tag="LP327_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726350.1"
                     /translation="MTADERIATALESIANSLSTLAEDTKANKELKETLITNVNDMQK
                     AIETLKEDPFGLKRR"
     gene            5051..5236
                     /locus_tag="LP327_002257"
     CDS             5051..5236
                     /locus_tag="LP327_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726351.1"
                     /translation="MDVIYFYKLDEINIPIFVSRTSDISLNLFDDVEAQKIVNEFPEA
                     RNNLYILVGTTEFKLNI"
     gene            complement(5225..5617)
                     /locus_tag="LP327_002258"
     CDS             complement(5225..5617)
                     /locus_tag="LP327_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HBL1726352.1"
                     /translation="MKLNQDCVRDILLELEKKLDLNTQLYKNDLLTFSSYEKYGEKDF
                     FYTLLKLTEAGYLNAKVTAGANNPVFSVNISSISWEGHEFLDSVRDNEVWKQTKSIIS
                     KFSSVSITTIENIASNVITQLISKQLNI"
     gene            5757..5960
                     /locus_tag="LP327_002259"
     CDS             5757..5960
                     /locus_tag="LP327_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726353.1"
                     /translation="MTDIAEITQRDREKIKAYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            5980..6231
                     /locus_tag="LP327_002260"
     CDS             5980..6231
                     /locus_tag="LP327_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726354.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            complement(6479..6937)
                     /locus_tag="LP327_002261"
     CDS             complement(6479..6937)
                     /locus_tag="LP327_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726355.1"
                     /translation="MIKILSDYQVTITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            7099..7323
                     /locus_tag="LP327_002262"
     CDS             7099..7323
                     /locus_tag="LP327_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726356.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            7333..7527
                     /locus_tag="LP327_002263"
     CDS             7333..7527
                     /locus_tag="LP327_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726357.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDVILEYVQQHYPKIELGGI"
     gene            7530..8552
                     /locus_tag="LP327_002264"
     CDS             7530..8552
                     /locus_tag="LP327_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBL1726358.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQDEVTRVADVPGHSEIEQAHLIENEDTDPIQEELLDIPDFGREEGVDDVSEFE
                     DDEYPF"
     gene            8515..9330
                     /locus_tag="LP327_002265"
     CDS             8515..9330
                     /locus_tag="LP327_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HBL1726359.1"
                     /translation="MSQNLKTMSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWSPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPHGLLKDFQIAE
                     QMIERLKQEEVFMNIYQGEKEMIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKTFTPIIAAVSKQTPSEARLITI
                     DQDNINYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            9346..10131
                     /locus_tag="LP327_002266"
     CDS             9346..10131
                     /locus_tag="LP327_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726360.1"
                     /translation="MTGGWIKLYRQLLEKPIWQESTPEQKVILITLLSMANHEEREWE
                     WQGEPYKASPGQFVTSLDSIRKKCGKGISLQNVRTALKRFEKYEFLTNQPTNKNRLIT
                     IVNWEVYQSKDNDLTKELTSNQQATNKQLTTNKNDKNDKNDKNDNNPRNSRKTREYAD
                     DDPNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSND
                     FWSGVILSPTSLRKHFDKMAIQKNKRKQQNISNDELPETGEDW"
     gene            10131..10979
                     /locus_tag="LP327_002267"
     CDS             10131..10979
                     /locus_tag="LP327_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL1726361.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10976..11218
                     /locus_tag="LP327_002268"
     CDS             10976..11218
                     /locus_tag="LP327_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726362.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11361..>11721
                     /locus_tag="LP327_002269"
     CDS             11361..>11721
                     /locus_tag="LP327_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBL1726363.1"
                     /translation="MATRSKYGNKKHEVDGITFDSIAEAEYYDLALWQAEANGWKVKL
                     QERFELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEE"
BASE COUNT         4070 a         1991 c         2203 g         3457 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tgatatttta gatgaagata agcgaattta cagcattttt aaatacttat
      181 ataccgcaaa ttataccaag tttttatata tcctacccga atttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ctaatcgttt ttgaagctct tttggggtca tattagggtc actaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gtaaagttcc gtcttgtttt gtatcaaata
      541 taaaatagtg tcttaatttc tttatattta atttccaacg aattttttta ttctctttga
      601 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tacttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagaat ttctcccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattagg gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaactcacca aaagttatat
     1261 tgaataaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctattcgctt ttatagtttt gctttttctt tttgccgatc
     1381 catcaggttt gtatcctaat acagctctta gtctatattt ttcattgtct acttgttcta
     1441 aatatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggaact
     1501 ataaagaagc ctatcctata ggtttgtttt ctagcgcgtc ccaaactgtc caaggtgggg
     1561 gatgtgtttt ttataggttt agtaattctt ttttcttagc atcgaattcc tcttgcgaaa
     1621 taattccttc gtcaagcaac tctttaaatt tacgaatctc atctgctgat gtagtcggtt
     1681 gttctttttg cacatttgta gaactgacta attcatcatg tctatctact tctaattcag
     1741 aaacaatata atcaaagcca gccatagttt cttttgcctt cattaacgtt ttttcgtatt
     1801 ttttgtcttt ctcttgtttc ttcttgatgt agtctaaagt tactgactga ggagttcggt
     1861 tcttaaaagt aaccatgatt ttcaaagagt caacataatt tctttgtttc tttttctttg
     1921 taactcctcc taaaacagcg ccgacgccac cagcaagaac gccaccgaca acagcccgtc
     1981 caattcctaa tccaccactc ataacggagc tgccgttttc caaaagctca tagctattta
     2041 attcatcgaa tgtataaaca ttgagtttaa ctcgaaataa tttttttaca gaatcaattt
     2101 caacttcgcc gtatctatta gtagatttga aataacgagc cggtttcttt gcaatagctt
     2161 cgtctggagt cattttggta aaaccaacaa attcttcaag ttcatctgaa gacagttctt
     2221 tattattttg aaccatattt tctaatgatg attgtctggc gatgtccttt gcgcctgatt
     2281 ttacagaatc ccaaaatccc attttttata gcccctcttc tgatataata tgtttgtacg
     2341 actctgaaat gaggaaccct agccggagcg ccaactccga ctggggtatt tttttataaa
     2401 acgactacgc cataaatttt aaaatcatct gtatcacaga ttgatatatc ctcatacttt
     2461 ttatttaaac tcactagctt gttacctgac agttttttaa caaacgcttc attatttact
     2521 tggcatacga ttatctgacc gtcacgcaca tctgatgtgc ttttaataaa gataacttgt
     2581 ccatcttcga aaagcggtaa cattgaatca ccgtttacct taactgcgaa atcatgttca
     2641 ggtatcacgc cttcataact tacttcgtcc acaatttcat cttctagcca ttcacctgtt
     2701 ccagcggaca cataacctcg aatttcaatt gtagttttcg gacgtttgtt ttgttctctc
     2761 aattgatatt ccgcaaagtt gtagactttt gtttgtcttg gtttttctaa ttgattgtag
     2821 attgtttcaa tggatggaat gttatttttc ttatcttctt taggaacatc gtaaccaagc
     2881 aaccatgcct cactcacatt taaagtttta gccaacaaat aaagcttgtg ttggtcgggc
     2941 gatgacttac cattgacata ttgcgataga tgactttttg acatttttat tccagtttct
     3001 ttttgcaaag gtaaagacat attcaaaata tcaacttgct ttaaatttct ttcagacatt
     3061 aactgattaa gtcgttgctg agtcgtttgc ttcatcttat acctcctttc tgatacttat
     3121 tatatacctt attgaacata agttcaataa aaaagttaaa ttaatttgaa cttttatgtt
     3181 gacaatgttt tatgcaggag ttatactttg gttacaaagt tcaaaacatt tgaactagaa
     3241 aggagcgaac taatatgagt tttgattatt ctaagttgtc gggaagaatt gttgagaaat
     3301 atggaacgca atacaatttt tcaatcgctt taggtttgtc ggaaagaagt ttgtcattaa
     3361 agttgaataa ccgagtacca tggaaaagca ccgaacttca aaaagccatt gaattgctag
     3421 atattccaaa agaagaaatt ggcgaatatt tttttaattt agaagttcaa aatgtctgaa
     3481 cttaagaaag gaattcgaaa aaatgaaaac acaaaaattt gaaagcaatc tatttgaatt
     3541 agaagttaaa acggaaaatg gtgaatcgtt atttaatgct gaaacagtag caagaagttt
     3601 gggtttcgtg gaaacaaaaa acggtaaaga atatgttcgg tggagaactg tcaatggata
     3661 tttaaaaaaa tacctttcgc aagaagttgc gaaaaacgac ttcatcagtg agccaatggt
     3721 ttacaaatta gcctttaaag caaacaacgc tttagcagat aaatttcagg attggctagc
     3781 tgtagaagtc ctacctcaaa tccgaaaaca cggcatgtac gcaacggacg acctactaaa
     3841 caatccagac ttgctgattg aagtagcaac caaactcaaa gaagaacgca cgttacgatt
     3901 ggtagctgaa caaaaggcgg ctgaaatgca gcccaaggcg gactattacg accgaatctt
     3961 gaacaacaaa ggattagtta cagttagtac gattgctaag aactacggta tgagcgctgt
     4021 atcgtttaat aaattgctac atgaattagg tatccagttc aatcaaagtg gcacatggct
     4081 gctatacagc aagttccaag acaaaggata cacgcatatt gagccatttg attatgaaga
     4141 taaaaacggc aatcggcaag taaaaacaag gatgaaatgg actcaaaaag gacacatctt
     4201 cttatatgaa acattgaaga agaataatta cttaccaatg attgaacgtg aacaaacagc
     4261 ataggaggta acactatgaa cgaggaaatg agccaaacaa tcaaggtaca aaaaatgata
     4321 gatgatctaa cacatgcaat tgatagccaa gccgataaaa tcatcaagga attgcaagga
     4381 caaaaagttt gggatgcaaa aatgtttctc aagactatca aatttgaaat gaatccaaca
     4441 aaaagaaagc tcgccgatgt gttggaagaa aagttggctt ccgctatcaa cgagcaagaa
     4501 ttactatttg aaaccgatac ttttaacagc tgatgtttta tagatgacag tttcatccga
     4561 atctatatca tcgccgagag aaaagaatgt tgcttttgac aaaaagccga taagacctac
     4621 aagcggtctt gatgttgcaa gagtagatac cccaatatca ccttgcaagg tattcacgta
     4681 aatttgagaa gaatagaaca ttccatcttt ttcttcgata tttgacgcat catcgtatcc
     4741 aacgaccaaa gtatcggcaa atattttgat tttttcaagg tcgccactga ttttaacatt
     4801 ggtttttcta ctcatagatc cacctcactt tctacagtga gtataccaga gaaggaggta
     4861 acagcatgac tgcagatgaa cgaattgcaa cagcattaga aagcatagca aatagcctta
     4921 gcactttggc agaagatact aaggcaaata aagagctaaa agaaactctt attacaaacg
     4981 ttaatgatat gcaaaaagca attgaaacct tgaaagaaga tccatttggt ttaaaaagga
     5041 gatagatagt gtggatgtaa tatattttta caaattagac gaaatcaata taccaatctt
     5101 tgtttcacgt acaagtgata tctctctaaa tctgtttgac gatgttgaag ctcaaaaaat
     5161 agtaaatgaa ttcccagaag caagaaacaa tttatatatt ctagttggaa caactgaatt
     5221 taagctaaat atttaattgc ttacttatta attgggtaat gacgttagaa gcaatatttt
     5281 cgatagttgt tatggacaca cttgaaaatt tagaaatgat tgactttgtt tgtttccaaa
     5341 cttcgttatc ccgaacagaa tctaaaaatt catgtccttc ccaagatata gatgaaatat
     5401 taacagaaaa taccgggtta tttgctccag ctgtgacttt agcatttaga tatcctgctt
     5461 cagtaagttt caatagggta taaaagaaat ctttttcccc atatttttcg taactagaaa
     5521 acgtcaataa atcatttttg taaagttggg tatttaaatc taattttttc tcaagctcta
     5581 ataaaatatc gcgaacacaa tcttgattta gtttcatatt ttttcaccac cttatcactt
     5641 atttcagtag accacttact gataaggaaa ttataccaaa gaaaggaagg actaaaaaaa
     5701 gaaggatatt accctcaaat ggatttagag gaaattggtt agaaaggagt tttagtatga
     5761 ctgatattgc agaaatcact caacgagata gagaaaaaat caaagcatat gtcgaaagtt
     5821 caaagttctt aacttacacc atgctcgctg aaagattcgg cattagcaaa agctacttat
     5881 ctttaatttt aaacggtaaa aagacttctg cagaagcaaa tagaattata gattcgatta
     5941 ttactatgta cgaattataa atacaggagg aattaatcaa tggagcaact agctttagtt
     6001 aacctctcag atctcaaagc ttctttggca gaatctgaga ttgcaaatga agtgtgggat
     6061 acaaagcaag ctgcggaata tttaaaaacc acaacacgca cgctgactaa agatgccgaa
     6121 tctggaaaga ttccagcagc gaaagttggc agagagtgga ggtttagtag tatcgctttg
     6181 tatcagtatc taaaaggagg aaaaacatgg gtaaattcaa cagagcatta gtattcagcg
     6241 caccgctaat catctacgct ttaggacttt ggggaagcag gcaagcgttg ataggaacga
     6301 tcgtttacat ggtctggatt tttatggggc ttgatgaagc tgagtacaga gcgaaaaagc
     6361 cagtcgggag ggactgacta atgagtaagg acgatattgt atttctgtta attatgtttt
     6421 gtgggattgg atcaataata attaatttgt atttgattat taaagaaaaa tttttcaatt
     6481 aaaatgagtc ttttagcttt aacttaaatt ttttttgata attagctgtt cttatgacca
     6541 ttacaagttt ttcaccaggt aatatatctt tcatatctaa aacttctgaa aaagcgactg
     6601 agtagccagg agagattgct tgtgccaacg gtataggaga taaaaggcta cttttatgaa
     6661 tgcttcccat atcaaaacca ttggcgtgat aatgtgaaca attaattttc ttaccgttgt
     6721 aaagatacgt acattcgata attgagtttg gcatttttga tgggtttgca attacgccag
     6781 taagaatcat tcgattactt ctatcatgct taactaaaaa ataggagagc gtaatttttc
     6841 ttttatcttt aaataagtta tagagggata aaaaaaatcc tgaaatagaa atgattaaag
     6901 taatagtgac ttggtaatcg ctcaaaattt ttatcaaata attcaccacc atttttttac
     6961 ctaaattata ccaaaaggag agaagaaata atgcaagaat tagtaatttt gaaaaataaa
     7021 aaaagacacg aacgccagca agcatgatcg tgtccaaaag aaaaatcttt taaaccatta
     7081 taacacagga ggaaataaat gatctcagtt aaagggttag gcgatgaaat attcgaagca
     7141 atgatgagca aagcacaaca ggacatcaaa gaaaaaatca tttctgctgc agcgcaagga
     7201 caaactagct gcactgttcg atcgagagga acgacaccaa gttttctttt ggcgctagaa
     7261 gatgaaggca ttttgaatat caaacgaaga gatggcagcg tcagattgtt ttggcaattt
     7321 taggaggccg tgatgaaaga ctttgattct ttaggtgcca gacagcaacc accaaatgaa
     7381 gctagtcctg tcggagttga ttggcaagaa aatccgttat atccaggtga tacttgctat
     7441 ctaacagaag aaggttatgt accagtagac gtaattctag agtacgtaca gcaacattat
     7501 ccaaagattg aattaggagg aatttaggaa tggcaaatga tttaacacaa acaacacaac
     7561 gttcattaga tgagcaggta atcagtaatc ttggacggtt acaagaacaa ggtttagaaa
     7621 tgccaccagg atatagtcca caaaatgcgt taaaaagtgc ttttttcgaa ctcactaaca
     7681 atacaggagg aaacttgttg cagatggctg caaacaatca agaaatgaaa acctccattt
     7741 ctaatgcact tttagacatg gttattcaag ggttatctcc agcaaaaaaa caatgctatt
     7801 tcattaaata cggaaacaag gttcagctaa tgcgttcata ttttggaaca atggcggtcc
     7861 ttgatcgtgt aactggaggg gcagatatta cgcctgtagt cgttcgacaa ggtgatgagt
     7921 ttgaagttgc aatggatgga ccaaacatgg ttgtcaaaaa gcatgaaaca aaattcgaga
     7981 atttagacaa tgaaatcatt gcagcctatg tagtcatcaa gttaacaaat ggtaaagaga
     8041 ccacaacagt catgacgaaa aagcaaatcg atcaaagttg ggctaagtca aaaatgaaag
     8101 gttctggacc gcaaaaggaa tttccagaag aaatggctaa acggacagta atcaatcggg
     8161 cagctaaaac attgatcaac acaagcaacg acaacgattt attagttcaa gctgcaaaag
     8221 atacgttaga aaatgaattt gacaatgatc ggaaagatgt aacgccacaa acagaaaaag
     8281 tggctactct cgaacaaaaa ttcttttcaa acaaaaagat tactgaacca atacaaaaag
     8341 aaccagatcc gattgtgatt ccagatgata tccaagatga agtaactcga gttgctgacg
     8401 tacctggcca ttcagaaata gaacaagcac atttaatcga aaatgaggat accgatccga
     8461 ttcaagaaga attattagat attccggact ttggacgtga ggaaggtgta gacgatgtct
     8521 cagaatttga agacgatgag taccctttct gatgaaaatt attactccaa tgaagctgac
     8581 tggcagtaca tgtcgacatc acaatataag tcctttttaa aatgtgaagc cgcagcttta
     8641 gcaaagttga aaggcgattg gtcaccgacg tcggatccca aagccttact cgtaggaaac
     8701 tacgtacatt cttattttga atcaaaagaa gtacatgaag cgttcaaaga agaaaataaa
     8761 tccaaaatgt tctctagtcg taagccacat ggtttgttga aagatttcca aattgctgaa
     8821 caaatgattg aacgacttaa acaagaagaa gtgttcatga acatctatca aggtgagaaa
     8881 gaaatgattg tgactgggga actatttgga acgacatgga aaggcaagat tgattgcttg
     8941 aatgttgagg atgagtattt tgttgatatc aaaacaacca aagacatgca tgagcggaaa
     9001 tggaacgaaa actacggatc aagagaaacg ttcattgtca attttggtta cgtacttcaa
     9061 atggcaatct atcaagagct tcttttacag caatacggaa agacgtttac accgatcatt
     9121 gcagcagttt ctaaacaaac accaagtgaa gcaagactga tcacgattga tcaggacaat
     9181 ataaattatg agctggttat gttaaaagaa aaaatcgaaa gaattgtcag agtgaagaac
     9241 ggtgaagaga agccgaacca ttgcggtttg tgcgagtact gtagaggtaa tcttccaatt
     9301 actggattca ctagcatgga cgatttgtag agtggtggtg atgaaatgac tggaggatgg
     9361 ataaagcttt atcgccaatt actcgagaaa cctatttggc aagaatctac accagagcaa
     9421 aaagttattt taattaccct tctttcaatg gcgaatcatg aagaaagaga gtgggaatgg
     9481 caaggcgaac cgtacaaagc aagtccaggt caattcgtta cttctctcga tagtattaga
     9541 aaaaaatgtg gtaaaggtat ctcattgcaa aatgttagaa ctgctctaaa gaggtttgaa
     9601 aaatatgaat ttttaacaaa ccaaccaaca aacaaaaata ggcttataac cattgtcaac
     9661 tgggaggttt accaatcaaa agacaacgat ctaacaaagg aactcacaag caaccaacaa
     9721 gcaactaaca agcaactaac aactaacaag aatgataaga atgataagaa tgataagaat
     9781 gataataatc ctcgcaactc tcgaaaaaca cgagagtatg cagacgacga tccaaataaa
     9841 aaattggcca ttcttttatt aaaactcatt cgaaaaaatc aaaacatcaa ggaacctgat
     9901 ttggataaat gggcgaatac gattcgttta acaattgaat ctgacaaacg aactggtaga
     9961 gaagttcaag acatgatcgt gtgggccaca agtaatgatt tctggtcagg tgtgattttg
    10021 tcaccgacta gcttaagaaa gcatttcgat aagatggcta tccaaaaaaa taaaagaaag
    10081 caacaaaata tttctaatga tgagttacca gaaacaggtg aggattggta atgaataaaa
    10141 aactaagtgc aatggctgca ccttacggcg gattaaaaac agcagatcat aattgcccga
    10201 aatgtggtga tccattgtat atctggaaaa caaaaaataa agatggtact gatcgatgcg
    10261 gtcctacatg tatcaataag atttgtggtt accgagaaat ggtaactaaa aatcaaaaag
    10321 aagctatcca aaaagcgaat gaagcaatga aaaaggacgc tatcaatcga atgcttaaca
    10381 gttcaatgat tacagatgat gccatatgga ccttcgattt tgatggatac aaagtagttg
    10441 atcaggaaac agcacaaata aaagcgatgg ctcaagaatg ggccaaaaaa attgtaaatg
    10501 gtagcacgat ccatgcagtg ataaccggta gaacaggagc tggaaaaact catctaggcg
    10561 ctgcagtgat taaagaagtc atgagggcat ctaattataa aattgcttgt tcatttatca
    10621 gttatcgaga acttttagaa cagttgaaat ttgcaatgaa tgatccagaa gcaagaaaag
    10681 ccgtaacagg ttcgctgatg gctgagataa agaaatcaga tttcgtggta atcgacgatt
    10741 tgggtgcaga actaggaaga atggaagaaa acaatcaggc aacgccttat gatgttgatg
    10801 ttctcacatc gctcacagaa gctcgtctaa acaaagctac gatattcaca accaatttat
    10861 catcgaagca attaaaacac gcgtacggtg agcgagtgtt ctctcgtgta atgaatggaa
    10921 cgaaagggaa catagctgta ttcaaaacaa cgacagacaa aaggaggaat ccagtttgac
    10981 ctttgtagtg aaaaaaatgt gctacttaga tagccgaggg cgaggagaag ctagtgtcga
    11041 gtttgctaaa caccacacga ctaaagaaga ggctgacctt gttgcaagtg tttgtggcgg
    11101 cgaagtagtc gaagttatta aacccaaaag acgattttca aaacctaaga caaagccagt
    11161 gaaaaaagag tgttgttgtc cgaaaagtaa ccaagcatgg atgagaggtg caaaatgact
    11221 tgtttaaaat gcaacgatga aacagttatt tggtataaga catcgcttgg atggtcaact
    11281 tgtgaacctt gtccaatatg caatgaaaat ggacgacgtt cgaaagaacg actcgaaaga
    11341 ctaaagaagg agtatagcaa atggcaacaa gaagcaaata cggaaacaaa aagcacgaag
    11401 tagacggcat cacttttgat tctatcgcgg aagcagagta ttacgatcta gccttgtggc
    11461 aagctgaagc gaacggctgg aaagtaaaac ttcaggaaag atttgagctg atgccgaaat
    11521 ttgaactaga cggaaagaag tatcgcaaga tcgagtatat tcctgacttc acattttata
    11581 aaaacggcaa acttgtcaaa gtcgtagatg tcaaaggaat gcagacaaaa gactttaaga
    11641 tcaaggcaaa gttgttctgt catcaatatc aagtgccgtt gatattagct aaaaaatatc
    11701 ggaatacgtt caaggaagag c
//
LOCUS       DAFEHS010000077        11578 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFEHS010000077
VERSION     DAFEHS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.080"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(10..159)
                     /locus_tag="LP327_002270"
     CDS             complement(10..159)
                     /locus_tag="LP327_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726364.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            555..755
                     /locus_tag="LP327_002271"
     CDS             555..755
                     /locus_tag="LP327_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL1726365.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1323..1886
                     /locus_tag="LP327_002272"
     CDS             1323..1886
                     /locus_tag="LP327_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726366.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1947..2291)
                     /locus_tag="LP327_002273"
     CDS             complement(1947..2291)
                     /locus_tag="LP327_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726367.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2388..2633)
                     /locus_tag="LP327_002274"
     CDS             complement(2388..2633)
                     /locus_tag="LP327_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1726368.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2703..2957)
                     /locus_tag="LP327_002275"
     CDS             complement(2703..2957)
                     /locus_tag="LP327_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726369.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3121..3444)
                     /locus_tag="LP327_002276"
     CDS             complement(3121..3444)
                     /locus_tag="LP327_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726370.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3631..3704
                     /locus_tag="LP327_002277"
     tRNA            3631..3704
                     /locus_tag="LP327_002277"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3665..3667,aa:Ile,seq:cat)
     gene            complement(4089..4586)
                     /locus_tag="LP327_002278"
     CDS             complement(4089..4586)
                     /locus_tag="LP327_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL1726371.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4626..5435)
                     /locus_tag="LP327_002279"
     CDS             complement(4626..5435)
                     /locus_tag="LP327_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL1726372.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5769..6626
                     /locus_tag="LP327_002280"
     CDS             5769..6626
                     /locus_tag="LP327_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL1726373.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6619..7521
                     /locus_tag="LP327_002281"
     CDS             6619..7521
                     /locus_tag="LP327_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL1726374.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7554..7904
                     /locus_tag="LP327_002282"
     CDS             7554..7904
                     /locus_tag="LP327_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL1726375.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7953..8876
                     /locus_tag="LP327_002283"
     CDS             7953..8876
                     /locus_tag="LP327_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL1726376.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8954..9847
                     /locus_tag="LP327_002284"
     CDS             8954..9847
                     /locus_tag="LP327_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL1726377.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9885..10448
                     /gene="amaP"
                     /locus_tag="LP327_002285"
     CDS             9885..10448
                     /gene="amaP"
                     /locus_tag="LP327_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL1726378.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10462..10650
                     /locus_tag="LP327_002286"
     CDS             10462..10650
                     /locus_tag="LP327_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL1726379.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10663..11565
                     /locus_tag="LP327_002287"
     CDS             10663..11565
                     /locus_tag="LP327_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL1726380.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
BASE COUNT         4104 a         1959 c         2171 g         3344 t
ORIGIN      
        1 ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg taacacctgt
       61 cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa
      121 ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc tttctttaaa
      181 tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag tataccacca
      241 attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata aaagcaaata
      301 aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg attcgaagac
      361 aatagaagac aatgaatttt atatttcatt gcattatcct tgtgttatta gaaaaagcat
      421 gatatattaa atgagtaatc tattttgaaa cgtaatttga gcgatatatc tacatagata
      481 aaactccttt tgcctatcaa atatttattg caacgaaaaa acgtacattg atacgtatca
      541 ggaggaattt atttatgaat aacggtacag taaaatggtt caacgcagac aaaggttttg
      601 gctttatcac tggtgaagat ggaaatgatg tatttgcaca tttttcagct atccaaggtg
      661 acggcttcaa aactttagaa gaaggccaag cagtgtctta cgatgttgaa gatggtcaac
      721 gtggccctca agcaacaaac attgttaaag cttaataaaa attgactttt aaccgtcttt
      781 tcgaaagaaa agacggtttt tttgtacgaa aaaattcatt ggtcgttgaa tgggctgaat
      841 agtagtgtat aaaacaattt tgtggagcgt gaagctttta tggtagagga aaatacgaat
      901 aaagtgatgt tgataaaaag attcctatct tgaatatgaa tcgagatgtc ttgaaaatga
      961 atgagtttga ccaatcatta tcctatcgaa atttacagtt tcgttcgatt ggtctgtcaa
     1021 ttaatgaaaa agtaaaaaag aatatgttct aggaagaggg cggcacatct aaatttttaa
     1081 gtaacggaca gttcaaatat caagcattga aagaggctat cctactgttt gaaggaatac
     1141 gaatatataa aaacgacaaa gaggaaaaag tagttcatta aatcccttca agtgatgaaa
     1201 gaatgagatc taataaaacc tgatctttca tctgtttttt taataacgta ataatttttt
     1261 tattttttgt gctttcgccg ttattgtaga gtgatttagc attcttattt ttaaaaataa
     1321 ctgtgttata tattaaaaaa ggcataatag ttataagctt ttttaaagaa tatttagaaa
     1381 ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat tattttagtc tattttcagg
     1441 gggggaacct tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag
     1501 gttgttctga ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag
     1561 aggagttaaa atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg
     1621 gaatattagg cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa
     1681 gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag
     1741 acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag
     1801 ttggcgatga accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc
     1861 ctaaaactta cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga
     1921 ggagatagaa ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt
     1981 gctaaacatt ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct
     2041 acagtaggta aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac
     2101 tctggtgctg ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg
     2161 aataatctat aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag
     2221 ccatttcctc ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc
     2281 tttgaaatca atttttcatg ccataatcac atagaaatgt ctcctttata tatagattgt
     2341 taaccttaga cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg
     2401 cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata
     2461 tagattaatt caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat
     2521 aaaggaatac ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag
     2581 aaattcagac gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca
     2641 cctttcatta tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt
     2701 actcaatgtt ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc
     2761 agtcctgata attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact
     2821 ttccacataa agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc
     2881 caattcataa gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact
     2941 ctcaccaacg taatcatgta acggactcct caacgattca tataataaaa gataacatgt
     3001 tattgcatac atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa
     3061 tgaaaaagca gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat
     3121 ctatcgagaa aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac
     3181 gatcgcattc actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac
     3241 aaatactttt gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag
     3301 aagcaaaagg gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg
     3361 ataatttaat tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa
     3421 tggcaaaata atcactccta acattgagaa taaataaaag aataacattt tttctaaaat
     3481 aagcaaataa aaattaaagt aacaaaatag ttatgacaca agtaaaagaa ataagaataa
     3541 caatattatt ttacttatat tttgaaactt gtgagagttt gtcattcgtg ctatagttga
     3601 aaagtaacaa aaataaattt tatttatgat gtacctttag ctcagttggt tagagcagac
     3661 ggctcataac cgtccggtcg taggttcgag tcctacaagg tacataagag gttgtgaaaa
     3721 agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttact
     3781 tggctgttct tattactgca gatgcaagct tttgaatacc gtttaaggaa gaggttgtga
     3841 aatcagcgtt ttgacctgag tattagctta acaaacggaa aaatagttcc tcatattttg
     3901 ccgtttgtta agctaatacc gagggtcagc tgattgaaca ccatttaaga aagaggtcgt
     3961 gagcctgccg tttcactctg tgtcataaaa aagccaatct actttgatag aaaaagcacg
     4021 taggactaaa acagttctgc gtgctttttt tattgtgtgt taaatatcgt aaatgaagat
     4081 agatattttt atgattcgac gatagagacg ttgaaatctt tcaacagctc taaagcggat
     4141 tctccgtgtt ttctactgga gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa
     4201 gcactctgca ggcctatcag attagagagt acacacaagt ttgtttcaat cccgactact
     4261 tcgatctctt tttgttcttc tttgctttca aataaagttt cctggatttc agctaaagtt
     4321 tcaggtggaa tcgtaaagta atacttttta atcactcgtt cattttcttg tggagaaaga
     4381 agagggatca attgcaaatc ttctcgttct tcttctttgt ccttcagttc aactggtata
     4441 tcccgtgtga agagaatata ttcattattt tggcgtgctt tagctaatct ttgagcaata
     4501 cgttctgcca attgatctga ctctggaata taaaattcgc tgtttggatc aagaatatga
     4561 ttttgcatat caataataac taacattttc tttcctccta cattcatatc gtcattttat
     4621 tctttttaat agaagttttt taaaatataa gcaacaccag cttcttcatt cgtcagtgtt
     4681 acttggtttg caatttcttt tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg
     4741 ctgtaattca acatatcaat gtcgttttct tgatcgccaa aagccatgat gtcactcgat
     4801 tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag ctttagtgat acctgtaggc
     4861 gtgatttcta agaaatgcgg atcagagaat actgattgga taccatctgc ttgcagatct
     4921 ctttgtagct tctgtagttt ttcttcactc ttatctgtta atgctaattt aagatagcta
     4981 agagaaggat cttccaacat atttaaaatt tgatcaaaag aatggttctg taaattgaaa
     5041 tgttcataat aagggtcatc tttgactaga tttgattcat cgaatgtcag ctgatcatct
     5101 tgggtttctc ggctagcaat gatggatatt ttcgcttctt gagcatagaa gaaggctttt
     5161 ctcagtgtat cgctatctat ttttgaacgt ctgagaactt tcgccatttt tccatccttc
     5221 atttcagcaa tcagtgaacc attggaagaa atcaaatagc caccgattcc taattcggaa
     5281 aaaatatctt tagcagaaat gctgttcctt ccggtagcga cggttacgat tccgccattt
     5341 tcatcaaatt tccgaagtgc ttggatattt tcttctggta tctttccatt tcgatcaagc
     5401 aaggtaccgt ctaaatcaat cgctgctagt ttcattttat cttcctactt tctttttaca
     5461 tatctatttt cctgttacct taagattaaa tgagaattta aaaaaatgaa actaacttgc
     5521 tttgtcatta tgaatttttt caaaaaacat tcttaagaaa aaggtggcta tatatagtag
     5581 tttttggtca atacctttga ataaaaagcc atgaaaaacg aatagttagc gcttaccgat
     5641 gcgttttgtg ataaaaaaat tttaaaatat tcaatgaaaa caaagcatat attttgcttt
     5701 aaaaccaaat gaggaatatt ctagatgtag aacaggttag aaaaagatta gaaaaaggag
     5761 tgattttgat ggagaagaga tacggcgtag ttcctgtggt aactgcttca gacgaaaatt
     5821 atgctcctta cttgagcgtg atgatcgcaa ctgcattaga aaattgcaat aaaacaagac
     5881 gcataaaatt ttatgtgatt gacgatggtc tatcagaata tagtaaagaa ggattggaag
     5941 aaacggtaaa taaatattca agtaatgcaa gtatccaatt cctgacagtg gaaaaagata
     6001 tctacgaaga cttcctagtc agtgaccata tcacaactac cgcttactta agaatttctt
     6061 taccaaactt attagctaaa gaggattata agaaagtcct ttacttagat tcagatgtgt
     6121 tagtactaga cgatatcgta aaactatatg atgagccgtt aaatggaaaa acaatcggcg
     6181 ctattatcga tccaggccaa gtgaaagctt tggaaagatt aggaatcgat tccgatgact
     6241 tgtacttcaa ttcaggagtg atggtgattg acattgatca atggaataaa aaagaaatta
     6301 cagaaaaaac tatccattac ctcagtgaaa atggcgatcg aatcatctat cacgaccaag
     6361 atgctttgaa tgctgtactt tatgaagatt gggaacaact tcatccaaaa tggaacatgc
     6421 agacttcgct gatttttgaa cgtcatccag caccaaatga aaaatatgaa cgtcagtata
     6481 aagaaggaaa tgaaaaacct tcgatcgttc actttacagg acatgataaa ccttggaata
     6541 cgttgaaaga tcatccgtat accaatttat acttaaaaaa attagcacac agtactctaa
     6601 cgaaagtagg cgagatcaat gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca
     6661 aaattcgtac cacatttagc tgcattattt gtctcagtat tagataattg caacccgtct
     6721 aaatttgttc ggttctatgt gattgacgat gatatcgatt ttgaaagtaa acagctttta
     6781 cgcttctcag tgaaaaacgc acgaatgaac tcagatgtgg aattcttgaa aatcaataaa
     6841 gaattcttta caaatgtggt aatcagcgat cgtattccag aaacagctta ttatcgtatt
     6901 gcgattccgg aattgttccg cggcacagaa gttgaacgta tcttatatat ggattgcgac
     6961 atgatcgctt tacaagatat ctctaaatta tggcgtcttg acttcggtga ttcaatcgta
     7021 gcagctgttg aagacgctgg gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa
     7081 tcaatgcgtt atttcaactc aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa
     7141 aatattacgc aaaaagtatt agatttcatc gaacataatc cagaaaaatt acgattccat
     7201 gaccaagatg cattaaatgc tatcttacat gaccgttggt taccacttca tccgcgttgg
     7261 aatgcacaag gctatatcat ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt
     7321 gaatacgaag aaactcgcaa caatccttac attatccatt tttcaggaca tgtgaaaccg
     7381 tggagcaagg attttgaagg gccaacgaag aaatattacg aaaaatatgc agggatgacg
     7441 gctttccgtt gtgtagctaa attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa
     7501 acagatgtag ttgttaaata atagaagaca acaactgaag agggggagta ggaatggaaa
     7561 ttacaatgtc taaaaatgca gtagagacgt taattgaaaa ggttggggaa aatacaaaaa
     7621 tcgcactcgc gcttatcaat gattccgatc catttctaag ggacaaaggt gcttttgcaa
     7681 aaggcagctt ttttcaaatc attccttttg tttcggaatt tggagaatat gcgactaaaa
     7741 tcgagcatcc attattggac atctatacat ctaaattaga gcaaaattat ttcggtaaac
     7801 gattgaacat ggacttcaac aaacagcttg acagcttttc attagaaaat gaaatcgcag
     7861 tactcgatta taatatcaaa ttaaagaact gcttcttcag ctaatagttt cgagaaagtc
     7921 caaaaaggag gtatacattg agatactact ttgtggatga agaaaagaat gtaatagaaa
     7981 cagatgaaaa aaaatataat tggctcgtac ttgatagtgc aaagcccgaa gagattgagc
     8041 aagcaatcga agaattcgag cttcctgatg atattttcgt aggaaccgaa tatccggaag
     8101 aagtttctcg gatggagcgc ctttatgaga cgaaactaga gcatccattt tctttggtag
     8161 tgattaatct agatcagtca gataatcgta tcgaaaaaaa attaacgcca atatcttttg
     8221 tactttccga taatctcttg attacttgta tcgatagaaa aaccgaattt gtggatcggc
     8281 tgctgacaaa gcatggaaat catttaaact cgtttgaaaa gatcatcacg tatacattat
     8341 tagatattta cacccattac gtgaaagaac taagagagat gaaaaagcga attgacgctt
     8401 tggataaagc agctcgtaaa acaacggaaa atgaagaact gtataaacag gcagatcttg
     8461 aaagagacat cgtatatatc gatcatacct taagagatca acgggaaaca atggatcaat
     8521 tatggaattc tgaaactttc atcaaacgtt tagacgatga acagctactc tatgatgtcc
     8581 atttgagaca gcgacaaaca gaaaaaatga tcgagatcta tcgagattta ttagagagta
     8641 ttggtgatct attcaacggg atgatggaca ataatctaaa tcatatcatg aaatatctag
     8701 attcagctgc attaatcatt tccgttccgg cgttgttcgc agggatttgg ggaatgaata
     8761 caggaggatt accgggtaaa agttctgctc taggctttat actcgtagtc tttggctctt
     8821 tgatagcagc agtagtttta ggttaccatt tataccggaa agattataca aattaaagaa
     8881 tctatttaaa acaggtatca aacaaagaaa tttgttttgg caatacaatt taaaaactag
     8941 gaggaataca gcaatgacag aaccaaaatt aatcgacccg cgaaacttgt accatacgga
     9001 gaaatttcct aaacaagatc aggatacacc agctttacag cagaatatga ttcctgttcc
     9061 tgattgtgga gaagaatcct atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat
     9121 cactggtgga gattcaggga tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc
     9181 agatatcgct cttcaattct tcccaggaga agaaaaagat gcagaggaag tagcaaaata
     9241 cgtgaaagtt gcaggacgta agattgtctt actacctttt gatcttcgtg acgaacaagc
     9301 acctaaagaa atcatagata aagccgtgag tgaacttggc gggctagata cgatggtact
     9361 aaacgcagga cagcaaattt cttgtcagtc tattgctgag ttaccacttg agcaagtcaa
     9421 agacacattt atgataaaca tcatcgctat gtttgcttta gtgaaagaag ctgttccgca
     9481 tatcccagca ggaggagcaa tcgtaacaac aacttctgtt caagcattca atccaagcga
     9541 acatttatta gattacgcag caacaaaagc ttcaatcgct aactttacga tcggtttagc
     9601 aaaacagctt gcacctaaag gaatccgtgt aaatggtgta gcacctggtc caatctggac
     9661 accattacaa ctagacgaag gacaaccttc tgattcgatc cctgaatttg gtcagcaaag
     9721 tttactagaa cgtgcaggtc agccaggaga attagcacca gtttatgttt tcttagcatc
     9781 gaataaagca agttatgtta cagcccaaat ttacggagta actggtggag aagcgatcaa
     9841 cttataagaa gttgatcgtt tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa
     9901 aaacgatcgg agtgatcgga acattacttt tgctatctgt gcttttgttt gcgatactat
     9961 taagtgcaac ctatccattg ccttttcagc tagagaagtt tcgattcttt tccatcacta
    10021 attcatacgt gcaacaatat gtattttggc ttgccgttgc atttgctatt ttgacaatta
    10081 tcacgttact tgttttcctg ttttatccaa aaacaatcgg aaactttcta ctgaaacatg
    10141 gtgatgggga gttgacgttg gataaaaaag caattgaagg aatggttcgt tctcacttgc
    10201 atgaagatga attcatccat tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg
    10261 ccgtcaatgt aaaaggggag ttgaaacgaa cttcagcact cattggtaaa acaggaacat
    10321 tgatgaaaga aatcgaaaat gaagtacaac acatattggg aacaaaagag ccagtgaaag
    10381 tatcggtgaa atacacaggc tttgaagaac cagctaaacg aagcaatgac cgcaaacgag
    10441 tagaataggg ggggaccaaa aatgaaagaa ctattttcga catacaagct ccctatcatc
    10501 tttggggtac ttggattgat attagccgtt ttatggatca ccgtgggctt tttcaagacc
    10561 ttattgatct tgatcatggc agcgatcggc gtcgctatcg gtttttatct gaaacaaacg
    10621 aaaatcttag atcactattt taaatcttag gaggaaacat ccatgaataa caataacaaa
    10681 aataataaca ataaaatgaa caaccaaaaa ccagtgtctg cacatccaga cccaacgaca
    10741 ccaaaccaat caaatggtgt gaacgatgaa aagaacaaaa cagcaccagt atctgcacat
    10801 ccagatccag tagtccctaa cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa
    10861 aagcatgcac ctcatcatcc aggaactcca gaatctaaag ttccagaaca acatgatgca
    10921 atcgatcctt ctaaacctgt ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa
    10981 gaaaaactta gttctgacca cacaaacgga caaggaaaac ttggttctga tcatacaaat
    11041 aaccaaggaa agcatgattc tgaacaggaa gattcaatgc gcggtgagct gaattatgaa
    11101 gataaagttg tgcaaaaaat catcggaatc gcaattgaac aagtagacgg tttattatct
    11161 gcaaacggtg gtttcttctc taatgtggca ggaaaacttg tgaatacaga taatgtaacg
    11221 gctgggatcg aaacagaagt cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg
    11281 gaatatggca aagatatcga aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa
    11341 gtaaaaaata tgacacattt agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa
    11401 acaaaagaag aatttgaaaa agatcaagaa acggtgcaag acaaagtaac tgacgcagcg
    11461 aaaaaaaccg gtgaatttgc ttcgaatcaa acagataaag taaaatcagc agctggaagc
    11521 ggcgcacaaa aagtaaaaga aaatacagaa ccacgggttc agtaataagg aagtaaaa
//
LOCUS       DAFEHS010000078        11522 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFEHS010000078
VERSION     DAFEHS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.081"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            453..1025
                     /locus_tag="LP327_002288"
     CDS             453..1025
                     /locus_tag="LP327_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL1726381.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            1170..1874
                     /locus_tag="LP327_002289"
     CDS             1170..1874
                     /locus_tag="LP327_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL1726382.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            2102..2440
                     /locus_tag="LP327_002290"
     CDS             2102..2440
                     /locus_tag="LP327_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726383.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2425..3279
                     /locus_tag="LP327_002291"
     CDS             2425..3279
                     /locus_tag="LP327_002291"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL1726384.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3426..3659
                     /locus_tag="LP327_002292"
     CDS             3426..3659
                     /locus_tag="LP327_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBL1726385.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGVDKDSIKVLFNSSKV
                     KANFDSAVTSIEEIQKAIENVGYPVLKAKVKAA"
     gene            3857..5761
                     /locus_tag="LP327_002293"
     CDS             3857..5761
                     /locus_tag="LP327_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL1726386.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVDVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAVPVNKQADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVGTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEETEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGHMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            6071..6433
                     /locus_tag="LP327_002294"
     CDS             6071..6433
                     /locus_tag="LP327_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1726387.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILNASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIHVFLKLSENLSMKS"
     gene            6450..7058
                     /locus_tag="LP327_002295"
     CDS             6450..7058
                     /locus_tag="LP327_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HBL1726388.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGLRQIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVALVGEEEEEEEVVEKLES
                     RGTNRFFWTVALITIASGGDNLGIYIPYFASLSFSEIVTALIVFAISVAVLCYISYKL
                     AKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(7160..8068)
                     /locus_tag="LP327_002296"
     CDS             complement(7160..8068)
                     /locus_tag="LP327_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL1726389.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIVTVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(8110..8424)
                     /locus_tag="LP327_002297"
     CDS             complement(8110..8424)
                     /locus_tag="LP327_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL1726390.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8723..10654
                     /locus_tag="LP327_002298"
     CDS             8723..10654
                     /locus_tag="LP327_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL1726391.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSINGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10948..11298
                     /locus_tag="LP327_002299"
                     /pseudo
     CDS             10948..11298
                     /locus_tag="LP327_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            11374..>11522
                     /locus_tag="LP327_002300"
     CDS             11374..>11522
                     /locus_tag="LP327_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL1726392.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKT"
BASE COUNT         3755 a         1909 c         2412 g         3446 t
ORIGIN      
        1 gtatacccta aatagacatc ataataacat tgaaaaacat ggttgtcatt tttctagata
       61 aatggcataa attttcagcg ctaagtgaca aaagcaacta atttttcatg agagattttt
      121 aaatatctca atctatctcc aaaattagtt gtttttgtag ctatagttga ctatgaatat
      181 gacaaataat tagaaaaaac tgtgccaatt aagtataaaa atgacatttt aggccctact
      241 ttaatgaaat ttttgtttgg agggaacgac ttcattcctt tgattatttc agttgaattt
      301 aatagagaag cctggcgtct attaagaaat aattttcttg atgagcgtca atttttttaa
      361 cggaaatcta tcctatacta aattcagtaa agaaaaagga ggaatcaaag aaagctattt
      421 taaacaaacc aaaattatag gaggaataaa tcatgactga aaataaagct actcaagaaa
      481 gaacacccca agaaagattg caagaagaac aagaacacaa ggaacatgtt catcatacga
      541 aaattaatgc agcggccatt gcggatcatc ttttaggcaa catgcataca ttacatgtga
      601 aactgcacca atatcactgg tatgtaaaag gagccaattt ctttacgttg catgaaaaat
      661 ttgaagagtt gtataacgaa aatgaaaaat ggttcgacaa acttgcggag cgtctaatta
      721 cttctgggca taaacctgct tcgacaacgg ttgaatttga aaaatattcg atgctttcgg
      781 aagatgcagt taataaatac gctaaagcag aagacatggt tgaaaatatt atcgaggact
      841 tcagaagcac tcgtgaccta actattcgcg cgattcgttt agctcaagac gagggtgacg
      901 atgctttaga agatacattg attgctttta aaaatgactt agacaagaac atttggatgc
      961 tgcaagcgtt cattggtaaa gaagcattag aagatgatga cgctcttgat gaggatgaag
     1021 attaaggtaa aagtagaagc tgatttaggg attggaattc tttccagtcc tttttccatt
     1081 ttcttgatgt gcatcaattt ttttgtagta agagacttat ataataaaag cagcgaatat
     1141 aaacaaaata aataaagggg gagaatgaat tgactaaaca tacccatcat gaaattggcg
     1201 accacgcagc ctgtatccgc ttggttccaa tattcaatca cttagaggat agcgcgatga
     1261 attacattgc tgaaaaagcc cacacgaaac agtatcaaaa aggagagttt ctgtttcgtt
     1321 cacaagaaaa agaggatact ttatatatta tcaatcgtgg gaaaatccgt atttatcgat
     1381 tggcagactc tggcaaagag cagctagtgc ggatcttgaa ccctggtgat ttcaccggag
     1441 aatggacctt gttcaacccg gatgcagtac acgaggaata tgccgaagca acccgagata
     1501 ccgttgtctg tatgattcag caaaaagatg tccaagattt tctggaacaa tacccagcta
     1561 tttctttaaa actattggcg gaaatgtcta aacggttaga aagttcagaa cgtcaaacga
     1621 cacaagtagc cgtggagagt gtcattaccc gtttagtttt gtttttggca gaaaatgtgg
     1681 aacctgaaat gggcaactct cccaccatca ccctgccgat ggcaaaaaag gacattgctt
     1741 cctatttagg aacgacacct gagaccatca gtcgcaaatt tggagaattg gaggacgaag
     1801 gattgattca acagctgtct gggaaaagga tcaagattca ggatttagat gatttattgc
     1861 tttacagcga ataatcggac acactttttt ctacacgttg gtgttctgtc gctcttcatt
     1921 aacaacggga cagaactcag tgcttttgct tccatctgtc cgtttttggc ttttttaaga
     1981 cagaacgacc ttcattcatt ggaaaaataa aagcacaaac atgataaccc tcaagttttt
     2041 ttgttttatt tttctttaag atagggttgt cgaacaaaac gtaaaaataa gtagggtgaa
     2101 tgtgcattgt ttttgggtga tagttgccat cacgttgtca gcagcggggg cattctttgt
     2161 cgtccctttc accaagaaca cgattggcgg cgaagcaaaa acgtctaacg ttgttgaggc
     2221 agataggtta gctatggaaa aagaagagga agttgtaaaa ggagccggcg taatcggaca
     2281 cggaaaagtg gcgtattacg aagcgagtga ccagggagat aaaagtgtat tcactgtttg
     2341 gtttccatgg agcttagccg gaatcttgtt cagcatcacc atgactagaa tgacgcgaaa
     2401 aattgaagaa atggagcaaa tagaatggga ttcgtattag gcgttttgaa tccccatgtg
     2461 gtggagattg gttctgttgt gattcattgg tatggagtca ttattgcagc gggcatatta
     2521 gcggctctcc agttgagcac caaggaagca aagaaaaaag gactggaaga ggataccatt
     2581 atcgatatgg ccttatgggc aattccgatt ggactcctcg gcgctcggct ttattatgtg
     2641 ttatttgaac ttggttatta tctgcaaaat ccaggacaaa tccttgcgat ttggaatggc
     2701 ggcattgcga tttatggggg attgattgca ggagggggta cgctatattg gtatgcaaag
     2761 aaaaaaggta cttctttggc actcgttctt gatattttag cgcccaatgt attgttagcc
     2821 cagtccattg gtcgttgggg caattttatg aatcaggaag ctcatggtgg gccggttact
     2881 cgtcaattct tggaaaatct ttatttacct gaatttatca ttgaacaaat gaatatgaat
     2941 ggtacctact accatccgac ttttttatac gagtcattgt ggagtttgtt gggctttgtc
     3001 ctcatcgtca ctatacggaa ccgaaaacag cttttgcgtc aaggagaagt cgctttgagt
     3061 tacgttttgt ggtactcagt gggtcggttc ttcattgaag gtatgcgaac ggacagttta
     3121 tggattggcg agtggatccg cgtttcgcaa gccttgtctc tggccctgtt tattggcgcg
     3181 attgtagtat ggtttatacg cagacaagat tatccaccaa tttcttatta ttctgatggc
     3241 aataaggggc agaaaaagac tattaagatg gtgaattgag caaaaagata aaaagaagtg
     3301 caaaacttga tatgcgtcaa ttttcgtttg ctaaaagtga gctatactac agttgtaaag
     3361 aggaaaaggc agtgttcatt aatctgctgc cgataaaata aaaacacata aaggagatag
     3421 gaattatgga aaaagctata ttgcaattag aaacgttgtc ttgtccaagt tgtatgcaaa
     3481 aaattgaagg tgccgtaaaa tcagttaacg gtgttgataa agacagcatt aaagtattat
     3541 tcaactccag caaagtcaaa gccaattttg attcagctgt cacttcgatt gaagagattc
     3601 aaaaagctat cgaaaacgta ggctatccgg tccttaaagc aaaagtcaag gccgcttaac
     3661 agattaaatg aactaggcaa ttctggaaga aaagctattt ttccaggatt gctttttttg
     3721 ttgcaatccg attcgaggtg cttagaaacg ataaaagaga actttctaaa cttgatgtca
     3781 gtcaattaat tacttaggca attatcttat actgtgttta tcaataaaag agaaattaaa
     3841 tttggaggga aaaagcatgc aacaatatat attaagcaag aaaaatgtta tcaccgtcat
     3901 cagtggattg ttaatcgtac tcggtttctt cagtcacttt gttttagaaa acgtaggact
     3961 ttcagagtgg tctttgatca tcgcctctgt ctttgggatt acgccaattg ccattcaagc
     4021 gtttcaagca atgaaagtca aagtcatcag tattgatgtc ttagtcagta ttgcagcgat
     4081 tggtgcgctt tttattcaaa attatgaaga atcggctatt gtcacattct tattcttatt
     4141 cggacactac ttggaacaac gaacattgaa ccaaacgcga tctgctatca aagaattgac
     4201 tgaaatggca cccgaaagcg ccttgaaaca aatggataat ggcgaatttg aagaagtaga
     4261 cgtggatgat atagatgaag gggatatctt actcgttaaa acgggtgcga aagttccagt
     4321 agatggcata gtccttacgg gtgaagggca tatcaatgaa gctagcatta caggggaagc
     4381 tgttccggtc aataagcaag ctgatgcaga agtttttgca ggaaccattt tagaaaacgg
     4441 aacgattcaa atcagagctg accgagttgg tgaggacacc acatttggta aaattattga
     4501 actcgtggaa gaagcacaag attctaaatc cgaagcagaa cggttcattg atcgcttttc
     4561 taaatactac acaccagctg tcttggttct ggcaattgtt gtatgggcgt tcagtcaaaa
     4621 tattgagtta gcaatcacca tcttggttct aggttgccca ggcgcattag ttatcggagt
     4681 gcctgtctca aacgttgccg gtattgggaa tggtgctcgt aacggtgttc ttttaaaagg
     4741 gagtgaagtc attcaagact tcagcaatgt gggtacaatt gtatttgata agacaggtac
     4801 tttaactgtc ggaaacccaa ccgttgcagc gactgaactg tatgaaaaag atcctgctga
     4861 aacgcttggc tatctggcca gtgtggaacg ggaatcagat catccattag caaaagcggt
     4921 cttaaatcaa attggagaaa caaactttta tcctgttgaa gaaactgaag tcgtgaaagg
     4981 cggcggaatc gtttcaagtg tagctggaca tagagtggct gttgggaatg tggccttgat
     5041 ggaaaaagaa aatgtcactc ttagcaaaaa agtgcaaaaa gatgtcaaac ggtttgaaca
     5101 acaagggaac tctctcgttt tgacagcggt cgacggtgaa ttaaaagtac tgatgggcat
     5161 tcgcgatcaa gtccgtccgg gtgtgaaagc taatttgcag gaattaaaag acttgggcgt
     5221 gaaaaatcta gtcgttcttt caggagataa ccaaggaacc gttgatgtgg tcgccggcga
     5281 acttggtttg accgaagctc acggccacat gttgccggaa gacaaatcgg cttatatcgg
     5341 aaaacttcaa caacgtggtc aaattgtagc ctttgttgga gatggcgtta acgatagtcc
     5401 gtccttagct ttagcagaca ttggaatcgc aatgggaagc ggaacggacg tagcgattga
     5461 aacatccgat gtcgttttaa tgaactcgaa cttcagcaac ttgcctcacg cattaggatt
     5521 agtaaaagcc accgcaaata atatgaaaca aaatatcgtg atttcaattg gagtagtgtt
     5581 ggtcttgttg accagcgtct tcttcagcga atggatgaat atgtctatcg gaatgttggt
     5641 tcatgaaggt agtatcttgg tggtaatttt caatggcatg agattaatga agtataagtt
     5701 gaaaggtcga aaagaacaaa aagaagtatc agcacctgca gagaaagtaa aaatatctta
     5761 agaataaatt agaagaggct gagacaatag tccatccttg aaatgacaaa agcggaaact
     5821 cagattttta aatctgggat tttccgcttt tttgagttct tgaaaaaaaa taagagtata
     5881 gagtatgttc tttataaaat taattttcgt gtaaatgatt aaaaaattta ataataaaat
     5941 ttaaattcct attaagttgt aacacatgaa atttcttata atagtgtatt gaaattaagg
     6001 tagattgaca aacgagtgat caaatagata tactaccttc aaacgaccgt ttgaatgtga
     6061 ggtgagatga atggaaaaag tttgccaagt gactatcgta cataaagaaa aagtaaatgt
     6121 aataaaaaaa aaattggatc aaaaagattt ttcaaactta cttgtgttag ggaaatgttt
     6181 cagcgattct tctagaatta aaatttttta tgctttagaa acctataagg aaatgtgtgt
     6241 ctgtgattta gcagaaatac ttaatgctag tgtcgctaca acgtcacacc atttacgttt
     6301 tttaaaaaaa catggaatgg caaaatcacg tcaagatgga aaagtagttt attactcttt
     6361 agcgaatgaa gacatccttt ccgctattca tgttttttta aagttatcag aaaatctatc
     6421 tatgaaatct tagtttggag gaagttattt tgttacaaag tattttttca gctatcgctg
     6481 tttatatttc taccagcatt gattatttgt ttatcttgct gattattttt tctcaaagtc
     6541 atacaaaaaa agggctccgt cagatttttt ggggtcaata tctaggaaca ggtatcttag
     6601 tagccgtaag tttatttgca gcatatgtgc tgaatttcat tccgcaagat tggatcattg
     6661 ggcttctcgg tctaattccg attttcttag gaatcagagt ggcattggtt ggcgaagagg
     6721 aggaagaaga ggaagtcgtt gaaaagcttg aatcaagagg aacaaaccgc ttcttttgga
     6781 ccgttgcctt aataacaatt gcttctggtg gcgataactt agggatttat attccttact
     6841 ttgcctcgtt atctttttca gagattgtca ccgctctaat cgtatttgct atttctgttg
     6901 cggttctttg ctatatcagt tacaaattag ccaagatttc ttttgtttcc gaaacgttag
     6961 aaaaatacga acgaatcatt gtccctataa tgtttattgc gttaggaatt tttattatga
     7021 tagagaacgg gacgatacaa acgctactag gtttttaaaa atcataatca cgaaagataa
     7081 aaagagaggc agcgattttg attagtcgct gccttttttg cttttcaaat aatgaagtat
     7141 tttttagcgt agaactatct tacgtgttgt gggagtatcc cttttcaagc tcttctttgc
     7201 ttgtagttat ttctattgtg atatgattct ctacgcttga tttgtgtgcg acttctgcaa
     7261 tttcttcttt tatttcccta gccttgtcta tgtctgaagg gtctataaga accgtgacga
     7321 taagggcgtt ttcttccccg tccagtgacc aaatgtgaaa atgtgagagg tcttttacag
     7381 caggaacatt tttaatggag tccgccaagc tctttacgtt tacattttca ggcgaaccat
     7441 tcaataaaat tttcattgtg gtgaaaaatt caggaacggt tttgtagagg atataaagtg
     7501 caatcaaaat cgataagata ggatccagac gatatgcctc ggtaaagttc attagaatac
     7561 tcacaatcaa gactccaatc caccctaata cgtcttccag catatggagg ttcaacatgc
     7621 tctcgttttt ggatgtgcct ttgcttaaaa tccacgcagc atatccattc aatgcaatag
     7681 ctacgagaga aagccagaac atgccactat aatttacggg ttgtgggtca atcaaacgtg
     7741 gcacactacg atagatcata aaaaacgagc cactaattag cacaacgcca gttatcagcg
     7801 cgcccagtaa agaaaagcgg ttatagccaa aagtgaaccg ttcatcttgt tttttctctg
     7861 aataaccttg aaaaaaccaa gcgaatccaa ttgaaataga gtcccctaaa tcatgaactg
     7921 cgtcggacat aatggacaca ctattgaaaa gaaagccaaa gaaaaattcg cttatagaga
     7981 aaataagatt tatgaagaaa gcaattttaa tattcttttt cggttgattc tgattcgagt
     8041 gattatggtc tttatctgat tttgacattt aaaaacctcc taagtttgta tgctacatac
     8101 taattggttt tatttttcta attcattaac atgagttaag acttgttcga gaatactaaa
     8161 aacatgaagg tcatctaaac tgtaaagcat actttttcct tctcttcttg atttcactaa
     8221 gcgtgcgtct ttcaacgttt ttaattgatg agaaatagcg gactgttcca tgtccagaga
     8281 aatagcgata tttccaacac tacactcttt tttctgtaaa agaaagagaa ccgacaatcg
     8341 tgtaggatca ctaatcacct taaatagttt gcttattgac agaatagatc ctttatcgat
     8401 tggagaagtt gtttttatat ccatagtgtt tttattcctt tcatatgacc tttcgttcat
     8461 gtctatatta ccatatgagc gaaacaatgc taatcttttg tttacaaaag agaagtggcg
     8521 ttctctttat cttgacaaaa tgtatatagc gatataaaat gagtatacaa acatatgatt
     8581 aattattcat atgtaaagta gattcattac gatttacttt tggaataaac gaaaggagtc
     8641 agaaatagca gaaatttatg tgattcaaga agtaaatgat ggaaaacaaa ggaattataa
     8701 attatatagg gaggcaatag aaatggaaac tatccaagaa caacagtctc aagaaaagca
     8761 taatcataat cacaaccacg atcacgacca tgatcatggg aaaatgccag tcgttttgta
     8821 ctttgttggt ttagcattag cagtgattgc tctatttttg aatgaagatt atcagttggt
     8881 acaaaatgtt ttgttctcaa tcgctacaat cagtgccggg tatcacgtta ttcttcttga
     8941 aggccttgga gaaacaattg agaattcaaa agcacaaaaa aagttcatac ctaattctca
     9001 tattttaatg ggattagcgg caattggagc ttctttgatg ggtaattttt gggaaggaac
     9061 attattgatt cttatttttt ctggcgcaca ttttttggaa gattatgcag aaggaagaag
     9121 caaacgagaa ataacgaaat tactcgaaat gaatccaacg actgcccggt taatcctgcc
     9181 tgatggtagt acaaaaaatg tagatgtcag tgaattaaaa gtgggagatc aacttcaagt
     9241 gttgaatggc gatcaagtac ctattgatgg cgtcattttg tctggatcta catccattga
     9301 tgagtcgtct attaatggag aaagtatccc gaaagagaaa tccaaaggag atggcgtctt
     9361 cgggagtaca atcaatggaa caggtacctt cacgatgaaa gtcaccaaag aaaacaaaga
     9421 tacagtattt tcaaaaattt tacaattagt gaatcagaac caagataacc aaacaaaagc
     9481 tgcgagtatt atccaaaaat ttgaaccgaa atatgtcaca gttgttttaa ttgccattcc
     9541 tctattcatg ttattggctc cttttctact tgattggaca tggtcacaaa gtgtctatag
     9601 aggattggtc cttttagttg cagcttcacc gtgtgcttta gcagcagcta ctgtctcggc
     9661 aacgctatcg acgacttcta atttagccaa aaaaggagtc ctttcaaaag gtagttctta
     9721 cctgtctcaa ttagccgata ttaaagcaat tgcatttgat aagacgggca ccttgaccaa
     9781 aggaaaacct gaagtaacca attattattt tgctgattcc gtgaacgaag aaaacatgat
     9841 tgatatcgta gtagctcttg aaaaagaatc caatcacccc ttagcagatg ctattctaag
     9901 aaagtttgag caaaaaaaca aactaactat tgaagtagaa aaccaaattg gtaaaggatt
     9961 gacgggagat tacaaaggga gaaattatcg tataggaaaa ccgacttcgt ttgatgatgt
    10021 ttcagatgaa tatagtcgtt taaataatga atgggcgtca gaaggtaaga cagtcgtata
    10081 cgtagcagta gatgaagagg ttataggcct tattgccctt atggacgttc cgagtgaata
    10141 tgctaaagaa accattgaat attttaggaa acaaggtata cacaccacat taattactgg
    10201 tgactcagaa atgacgggaa aagcggttgc taaacaatta ggaatagatg aagtgattgc
    10261 gaatgtcatg ccggaagaca aatccagaat tatagacgaa caaaaagaaa aatacggcgt
    10321 gaccgccatg gttggagacg gtgtaaatga tgctcctgct cttgttaatg cagatgtggg
    10381 aatcgctatg ggagatggga ctgatgtggc agtagaggta tctgatttag ttttaatgca
    10441 aaacaattta tctaaattgg tacaatctca taatatttct tcgaaaatga accgtgttat
    10501 ttggcagaat attatttttt caatggccgt tgttgctttt ttaattgtcg ttagtttctt
    10561 aggcttaacg gatattgcaa tcagtgtcat tattcatgaa ggaagtactt tggttgttat
    10621 tttgaatgga cttcgattgc taaaggcagt ctaaagaatg attgcacaac ttcaaattaa
    10681 tcgagtaata gagataatta aggattacac tacatttttt tatctctatt acttgtttat
    10741 atttatctag atgttcaaag tcaaaatgat atatcaacca tactcttgtg gcgcgggttg
    10801 aatataagat acatgaaatt gtataaaatc atgtatcaag aatcctatcc ttatagggtt
    10861 cttttttttt actcccccta aacacacgat ttcgtgttgt aataactata atcatgtatt
    10921 cgaacagact aaaaaggagg gattgtcatg ggctataatg ttcagccact acggtcgcaa
    10981 caggagatta atgatttttt attttgcctg cgacggaata aaaatgcgga acgtgacgtt
    11041 ttcttgtttc taattggaat taacagcggt ctgcgcatgt ccgacatcgt taaactcaag
    11101 aaaaaagacg tgattacctc aaaaaacccg cgcatcgtgg agcaaaagac agggaaaaca
    11161 cgcattttat acttaagcag cttacaggac ttgattcacg gctataccgc acccttagat
    11221 ccggaggctt acttgtttcc cagtactaag ggcggccatt tagaagtcaa tacggtctac
    11281 cagatgtttc aaaaggtctg gataggctac aattaactag acagaaaaat taaggtgtgt
    11341 agactagaag aaaacatacc aggaggaatt tttatgtcta agagaacacg aagaactttt
    11401 tcacaagaat tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa
    11461 atcattcgag aatatgaact aacggcttca gcatttgaca aatgggtaaa gcaatctaaa
    11521 ac
//
LOCUS       DAFEHS010000079        11519 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFEHS010000079
VERSION     DAFEHS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.082"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(67..1746)
                     /locus_tag="LP327_002301"
     CDS             complement(67..1746)
                     /locus_tag="LP327_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL1726393.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            2294..3796
                     /locus_tag="LP327_002302"
     CDS             2294..3796
                     /locus_tag="LP327_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1726394.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            3809..4471
                     /locus_tag="LP327_002303"
     CDS             3809..4471
                     /locus_tag="LP327_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726395.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4572..4775)
                     /locus_tag="LP327_002304"
     CDS             complement(4572..4775)
                     /locus_tag="LP327_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL1726396.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(4886..6070)
                     /gene="iadA"
                     /locus_tag="LP327_002305"
     CDS             complement(4886..6070)
                     /gene="iadA"
                     /locus_tag="LP327_002305"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBL1726397.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(6227..7966)
                     /gene="yfcC"
                     /locus_tag="LP327_002306"
     CDS             complement(6227..7966)
                     /gene="yfcC"
                     /locus_tag="LP327_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL1726398.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(8739..9353)
                     /locus_tag="LP327_002307"
     CDS             complement(8739..9353)
                     /locus_tag="LP327_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL1726399.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(9959..10336)
                     /locus_tag="LP327_002308"
     CDS             complement(9959..10336)
                     /locus_tag="LP327_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726400.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(10636..11517)
                     /gene="galU"
                     /locus_tag="LP327_002309"
     CDS             complement(10636..11517)
                     /gene="galU"
                     /locus_tag="LP327_002309"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL1726401.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         3440 a         2433 c         1948 g         3698 t
ORIGIN      
        1 atttgttttt cgcaaaaaaa gagcaagacg agcactctat agagaggttc atcttactct
       61 ttttgtttat tttttatttc gacgttgagt agcttccata atgactggca agatcactgg
      121 gcggcgtttt gtttgttcaa ataagtaacg gcctaattga tctcggatgt cttgctttaa
      181 tttgctccac tcaaaatcat tgctttctaa atgtttctca acgattgttt caatcatatt
      241 gctgctttct ttcatcagat cacggctagc ttttacgtaa acgaatcccc gtgaagtaat
      301 ttgaggtttt gaaatgattt tcttttctcg ccgactgata gtgacaaccg ctacgaaaat
      361 cccgtcttct gacaagattt ttcgatcacg taaaacaatg tttccgatat cacctatgcc
      421 taatccatca atcattacat tttctgcact tgtagttcct gcaacactca tacgcttgtt
      481 cttatattcc agcacgtctc cgcgtccagt aatatagata tttttgtatg gcatccctac
      541 tgcatgagcc aaatctgcat gagcagctaa ttcacgatac tctccttgaa ctggcacaaa
      601 atatgttggt ttcatcaaat tgatcattaa ttgcaagtca ttaggattgg catggccaga
      661 aacgcgcata ttctcagaga tcaacttcac tgttccacca gctcgataaa tgatatcttc
      721 cgttttcgcc acgatggttt ccatcgcgat gcttggcgta gtagtgatat aaaccaaatc
      781 tccttcttca atcttgacat tacggtgtgt accgtttgcc attttttgca gagacttgat
      841 gggttcaccc attcggccag tttctaaaat gatcagttct tctggatcat agtttttcat
      901 gtctttcaac gtaacaagca gatcttcact tggtaattga agtttttcta aacgcatcgc
      961 tgtgcgaata attcgttcga aatcttgacc agtcaagaca acttttcgtt gtgctttttc
     1021 tgctgcatcc aatacttgtt gcactcgttg caaattactt gctacacaag cgacgatgat
     1081 acgcccttcc caatatttga tcgtatcaaa tacttcatct gctatttggg cttcagaagc
     1141 tacaggctga ggattttctg catttgccga ttcacttagt aaggctaaga ctccttcttt
     1201 gccgatttcc gccaatcgtg cataatctgt ttgatacatt gggatagccg tttggtcaaa
     1261 tttaaaatct cccgtataaa cgatactacc ttcttttgtt ttcagattga tccccactga
     1321 atctggtatc gtatgagtgg tacggaaaaa gctcactact gtttcaccaa agtctatttc
     1381 tgtatgctca tctaccacat ggaaatcctt aaatcctttg gctgcttcat tacgactgac
     1441 attcaatttt gctaactcga tcgtcaactc tgtcccgaat actggtacag aaatttttga
     1501 caacaagtaa ggaagtgccc cgatcgcatc tgcatgtcca tgtgttaaaa agacaccagc
     1561 tacacgatca atgttttctt ctaaatacgt aaaatcaggg attacgacgt cgatccccaa
     1621 caactcattt tcaggatatt ttagcccaca atctaaaata aaaatatcct cgcccacttc
     1681 tgcgacatac atgttttttc cattttcacg aacgccgcct aatgggataa ttttaatggt
     1741 actcacaaac ttcacctctt cttatgacat ccaagtttaa agatgtcgat tttcttcttt
     1801 atctaaatat atgagccatt ccaatgaagc atatgcaaaa tcactatttc tcaacttatg
     1861 gagaatcagc aggaaaatta ccagcacatc cgcgttctga cagttggatg ggcattttat
     1921 tttggatata cttcattaac aaaatggatc ttttgcgaaa cgctatagcc agaatactta
     1981 tataagtatt cagctgttcc ccattattat acaacaaata aactaaatcg ctagtatcag
     2041 caattgttag aacaactatg cacgtaaaaa tccctctgta gcatctagga tttcttttgc
     2101 tattcctctt tcatttcttc ttcgtcggtt tgaaacatac ttttttcatt ttaccacatt
     2161 ttttgcaata actatagtga ctttttatat atacagaacc tcatgttgat ggtgtgcaaa
     2221 cagcataaga tttaagctag ctttaagata ggttgtctat tatcaaatgc atctactagg
     2281 agggatgcaa aatatgaatt ttatgaaacg tgcatggaaa agtacaaaag taaaatgggg
     2341 acgttcattg ttattgtttg ccgtgttcac agccattctt atttttgtgc tagctggcct
     2401 cacgattcga agcgctgcag aagaagcagc gaatcaagca caaaaagaag ttggagcaac
     2461 agtgacttta agtgccaatc gagaagcttc atttaaaaaa caggaacaaa cttcttcaag
     2521 cggttctacc actgaagaac gtccagatcc aggcagtttt tctcttacac cagtttcctt
     2581 aacggacgcg gaaaaaatag ctgaattaga taatgtaaag agttattcct ttgaaagttc
     2641 cgcctcagct atggcaaaag atggaataac tgccatcact agttctgatt caagtgaaga
     2701 cagttcagaa actactgatg caggcggtga acctgctgga ggaatgggcg ggaatccgca
     2761 aatgactcaa gcagactttc aaatcagtgg tgtgtcagca actgcacaaa atagtaattt
     2821 ttctgatgga acagcaaaaa tcacggatgg tgaaggaata gatgacagcg acaaaggcac
     2881 aaacaatgta gtcattgatt ctactctagc agaaacaaat gacctggcag tcggagatac
     2941 tttcacaatc acgagtactg aagatgaaga taccacttat gaaatgacga tcaaaggaat
     3001 ttatgaatcc agcgaaacaa gcggcagtat gggtatgaaa ttcaacttca tgaatccgtc
     3061 gaatacactt tacacttctt acacttttgc aaatgaacta aatgggacat ctgacgataa
     3121 cacgatagat tctgctattt acactctatc tgatccagat aaaatggatg agtttctcaa
     3181 agaagctgag aagctgatcg acacagatac atttagctta caatcaaatg attccatgta
     3241 tcagtcaatg ttggaacctt taaacaacgt ggcaagtttt tctaaaaatg ttgttttact
     3301 agtggctgta gccggtatta tcatacttac tctgatcatt atgattacca ttcgggagcg
     3361 tcggcatgag ttaggcgtct tgctatcttt aggagaatcc agaagtaaaa tcattctcca
     3421 attgtttacc gaagtggcta tttgtatgat tcttgcatta ggagttgctt cgcttagcgg
     3481 aaacgtcgtc gcgaatgccg tgggacagca gctacttgac caacagacag aaaccacgac
     3541 acaaaaccag caagctcctg gcggtggaca aatgccaggt aacagacaag aacaaggcgg
     3601 tcaacgcgat ggtcaacaag gaaccacgaa taatccattt gctgtttctg atcaagtcag
     3661 cgaattggaa atcaatgtca aacctgcaca attgggacta ttagccggag tagcatttgg
     3721 aatcagtctg ttttctgtct ttttagcttc tatagggata ttaagattaa acccaagaaa
     3781 aatcctatta aactaaagga ggaatttgat gttacggaca gaacaattgg gattttggta
     3841 tcaagatccg caagatgcgc tttataaaga tgtagaactg aatttcgaag ctggaaaaat
     3901 gtacgctatt ttaggagcaa gtggttctgg aaaaacgact tttctttcgc ttatttctgg
     3961 actagatgca ccaaaagaag gaaaggtata ctatcaagat cgttcactga acaaaatcgg
     4021 gttaagaagt tatcgtaaaa atgatgtctc gattattttc caagcttaca acttgcttcc
     4081 ttatttatcg gctctagaca acgtcatagc agcaatggaa atcactaaga ccaagcaaga
     4141 gaattatcga caagttgctt tagatcgact ggctcgtgta ggaattgatg aaatattagc
     4201 aaaaaaacag gtgaccaaac tttcgggagg tcagcagcag cgagtcgcta tcgttcgtgc
     4261 tatctgctgt gaacatcaac taatcgtagc ggatgagcct actggaaatc tagatgaaac
     4321 aacttcacaa gatatcgtca aacttttcca agaaatcgcc catgaacaaa atcgctgtat
     4381 tatcatcgtg actcatgaga ttgaagtagc aaaaagctgt gatgaagtct ttgaactaaa
     4441 aaataaaaaa tttcaaaaaa tcgaagttta aaaaacagga taatgatcaa ataaaaaatc
     4501 cgaaccatat ctgtctcgca gatatggttc ggatttttta tttgcttgat tcaattagta
     4561 ataagtggtt tttatttttt ttcgtctttt cgagaaaact tgtctttgac ttcgtcgcct
     4621 aaatttcgag ctttttcttt gatgtcagca cctagtgatt gtgctttccc ttctgtttct
     4681 ttgctcttgt cgtctgttgc tttgccgtat gcttcttttc cttcaccgac tactttatct
     4741 ttcatatctt tagaacgtcc ttttaaatct gccattgtgc ttcctcctct tagtttgttt
     4801 attgatctac cttcagggta acatcaacaa cagatacaga caaacagaac gctcgttttc
     4861 taaaaaagat agttagagct gttgattaaa aacttttttc gaaagtcccc catactttga
     4921 cttctttttc ttggaccatt atttctcctt tagcgataac tgtatcaata tcaagcgttt
     4981 ctttatccaa gatacacaga tctgcatcag caccaatttc gatttttcct tttttatcca
     5041 atttcaatat acgtgctgga ttactggtta tagctcgtaa ggcgatttcc aaagggatac
     5101 tttctttttg cacagcttct ttgatcccta ctagcaatgc cttggcactt cctactccta
     5161 accctaggaa atgattgttt tcatcaaaat aaggcaagct tccttgtcca tctgaggtca
     5221 tcgtgaaatt atctaaagaa acatcttcct cgatcaatcg tttcagtgct ttgctgaagc
     5281 gaacttcacc atctgtcttc tcccaaaaat caggatcttc gctagcagtg aagtcaatcg
     5341 tgccaccacg tttggcaaat gccacgcaag cttcaaataa ttctggtgtg cgatttgcat
     5401 gagtaggcaa aaattgtgtg atcggaattt ctgtttcttc tacgattcgt gtcaatagct
     5461 ctagctttct ttttcctccg cctaagtgga cattgatgat cccagctttt ccggataaca
     5521 tgcctcctac acgagcatct gctactgcac gagcgaactc ttcaaaactt ggctgagaag
     5581 aacggtgatc ggatactgcg atttctccga ttccgataat tttatcaatt gtcaaaaagt
     5641 ctttgatgat agaatctgtt actgttttta ccggcaatcg gtacgaacct tcgtatacat
     5701 aagtggagat tccttctgct tccagacctt tcgctttgct gatcaacgca gtcatatctc
     5761 taccttctcc gtctgttccc aaacaaccga caactgtcgt tacaccagct gtagtcaatt
     5821 ggctcaatgt gacttctggc gttcgatttt gataaccgcc ttctcctcct ccacctaaaa
     5881 tatggaaatg acagtcaatg aaacccgggg ttagtacttt tccctcacct gaaagttctt
     5941 ctacttccac tccttcgtag ccgccttcta gctgttcttc tacagccaaa atcttcccac
     6001 cggcaatcag tacatccatt tttcctaagt aatctggtgc aaacacttca atttgtcgaa
     6061 tgattttcat acatttttcc tctttcttta agtataactg atgataggat cttaaaaaat
     6121 aatccaaacg ataggatctt tactcctttg attttaccgt aaaatgacgg ttcttggagt
     6181 ccttatctac tgtttggatt acttttttat ttacaggtct ccagtactac tgcagaccta
     6241 ttgcaatggc aatcatgatg aaaatagttc ctaaaacaaa caggaatcct tgcatcttga
     6301 tttggaatct tgcccatttt gcccattcga tccgtgcaac acctagcaca ccaatcaagc
     6361 tggctgatac gggtgtaaaa gcgtccataa aacctgaacc aagctgataa gcaagaactg
     6421 ctacttgtct ggatacacct actaaatctg ccagtggagc catgattggc atagtcaaag
     6481 ctgcttgtcc tgaatttgat gtaacaacca agttgaacaa actttggaag atgtacatcg
     6541 cccaagcacc aatgacagct ggaacaccgg tcaacgccgt cccgatccca tgcaaaatcg
     6601 tattcaaagc tgaaggaaca gatgcatcag atcctcccaa gacaagaaga atccctttgg
     6661 ccatacctac tacgattgct gtaccagcta aatcagctgc accagattgg aaagaagaag
     6721 cgatgtcatt gactttcatt ccatctaatt tgaaaagaac agcaatgatt ccagcaacta
     6781 atcccatcac aaagaattga gaagcgattt ctggaatgta atagcctttt tgagtcacgc
     6841 cccaaacgat ccagacaagg acaactaaca tctctaataa gatcaattta tgcccaagca
     6901 tgaatttttt attttcatca gatgtctttt tgatatgaga acggaaataa tcatctgatt
     6961 tgtaagtcaa cgaactttct ggttttttac ggattttttc tgcatatatc atcatataac
     7021 cagcggctaa tgcagtaaca actacccaca taatcaatcg gaatgtcgca ccagacaaca
     7081 cggggattcc agcaatccct tgagccaccg ctacgctaaa cgggctcatc caagaggtcg
     7141 catttccgac ttgagatgcg acataagtca cggttactgc tacgatcgag tcatatccta
     7201 acgcaatgac aaatggaacc atcaccattg agaatggaat tacctcttct gccatcccga
     7261 atgtagcgcc accgaatgaa aaagcaaaga ataatagcgg taaagctaaa cgctccagtc
     7321 ccttagtttt actgataaac gcatagatac ctgcgtcgat cgctccggta cgcataataa
     7381 taccaaaagc acctccaaca acaaggatca atgcagcgat ccctacagca gatccatact
     7441 tatctccaga tactagtcct tcaaaaacaa aatttaagaa accaaagccg ccaaaatcat
     7501 ctgttcccca tattttggct gttttatgca attttttaga agtatcatag atattttcgc
     7561 cgtactgatc gtaaagcaca tcatctgtca aggagatttc atctagtttt tcctgcgtaa
     7621 gatttttttc tccgtcagca atgacttttt ccaatccttt ttccggaaca tccagttttt
     7681 cacgttctgc tggatgatct tgcagttctt ctaattgatc aaacacatac gacttatcca
     7741 gctcgtaagc ataacggaag gaatcttgac gtaaaacagt acgagtactt gtttcgccat
     7801 ttgcatcttt gtattcaata tcttgcgtgc tgaactttcc tgctggaacg acaaaagtca
     7861 gtatccatgc aaagatgact acaccaaaaa taatgacata cgtatggggt gtcgataatt
     7921 tagtgaagtc gcgtttcggt tttcctgaag aatttgtttt cttcaaattt atttcttcct
     7981 ttcttttttc agtaattttg atgaataaaa aatggataat caaatgaatt atacagtaaa
     8041 aatgacattt ccacatgaat aatttattag aaaatcactt ttttctattt tgtttttttt
     8101 ctcctatgta tgattatttt tttctctttc catttatatt aagtatttta tttctctgtt
     8161 tttccttaaa attctttttt tatttttaga ttagagaaaa tctcgtcctg tttttttcat
     8221 tttacaatca ttgaaaaatt gaatttcatt gcaaaaaaaa taataaacac ttataaatat
     8281 ccattatcta tccttcaatt tttgtgtatt ttatttctat tagaaaaagg tatcctgttg
     8341 gacaattttg ttatatcagg taaacttcat tgcaataatg ataacaaatg tactataatt
     8401 actttgtaaa gagttttggg gaagactctt tcatcaacgt gtattaagag acaagaaaga
     8461 atccatatgg attctttctt gtctcttttt ccactacttt ttatttttct gacactacat
     8521 attcttataa ctcatttctc ataactgtga aacaacttat aaaaataaca aactttaaac
     8581 aaaacaagct taaaatcaaa gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc
     8641 gttgttaatc ctcattaata atcgttttct ttttttcaaa cgctccattt ttatgtggaa
     8701 cattattttt ttaaggaact ttgtcaacat gtggataact attttccact tgtgtcctgg
     8761 actagctgtt ttgctgtaaa atacaaaact tgctgcagca aaaagatcgt tgcaagagag
     8821 gctgttccgc agatcgctgg atctagattt ggcaaagtca agactgcatc atgtaaattg
     8881 gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc gcaaaccact gctatgttgc
     8941 gatttgatac ttcgtgacaa atcaaatcca gtaacaatga ttaatggatg accattttct
     9001 ttgatccact gagctagttc gatgattgcc gggtttcttc cttcattcga aagaagaaga
     9061 aaaatgtctt ctgatttgac agaaagctga tcaataattg cctgtcctgc accttctagc
     9121 gtttcataga cacctccggc tggattttca atcaatatac ttggtacaag atccggggtt
     9181 tgatcccaaa attcaaatgc tacaccactt agtgtgcggc tagcatatat ctgcaatcta
     9241 ccaccttgat taatttgttt cgcgatccaa gtgccagctt gaagcatcag cggctcttct
     9301 atccttaatt tctcttccaa ttggtgataa tagtcaaaat aacataaact catttatctc
     9361 tcccgcctag ctatacttga catgaataga aacagctgcc taatctaagg aagtacataa
     9421 aaaaacagta gttagacagt aacacatgtg gtctttgctg tttcaaacac aaaaaaggca
     9481 ttgaacgaat acgcccaata ccttgacata gcaaataaaa caacgtgcag accatggaaa
     9541 atatctacat aggggtagaa ttttcaccct agattaggta caataaatta ggtcgatgca
     9601 ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt gtgtgactgg tcattccctg
     9661 attcttagcg ccaactaagg gtcagggagc ctttttttat tcagctgttt ctatctgaat
     9721 ttattgtaag taatctctta tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg
     9781 atttatatat atataaataa cataaaaaaa ggaatggctt atcccgatac aacaagcatt
     9841 cctttaaaaa agattctttt atataagtga aaaaagcttt tttccaccaa ttaaataaat
     9901 ctctcatctc aatagtttat ccattatttt atcttttttt gcgtatacct actaaatatt
     9961 accgtcttct tctcgttctt ctactttgtt cgagagaatc tgtactagaa aaagtagttg
    10021 tatgtctacc ttttctgcct atccgagact ttcttttcat atatgggata taaatgatta
    10081 gatacagtac agcaaaaaat aaagcaaata cgcaagcttc ccaactacct aacgccaatt
    10141 caaccatcaa ggtagcaacc ccaacggatg ctgcatacca gataaaaatc agaaacgttt
    10201 ctttttttct gaccatgcga aacatgataa atagactaat aagcaatagt ggcaacacaa
    10261 atatccaatt gcttccttcc atcagccact tgagtatatc aagcagcgac tgtgcaacag
    10321 cgactaattt tcccatagtg aacacacacc tttttttata attaaaaaat tcgccttttt
    10381 attctactat gaatcttctg gcttggcttt catttctctt atgtaatcat tttcttttat
    10441 acaacttaaa aaaaaattaa accattagca taatattttc tgaatctatt cacgaaagtc
    10501 tttttctatc tttcgttttc cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca
    10561 gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa tagcctttca ttctcagcag
    10621 gccacttatt tccagttaac tttctgttct tagcttttta ctcaactgga taatatatgc
    10681 acttaatgaa gtcccaattt ccgaatgttg aagtccatat tcaatatttg tcttcatata
    10741 tcctaactta tcgcctacgt cgtatctttt tcctttaaac tctctcgcat agaccgcctc
    10801 tgtctggtta agagtctgaa gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc
    10861 ttgagtttct aatatctcaa atattttagg agtcagtaga taacgtccga taatggctaa
    10921 attacttggt gcttttccca tttcaggttt ttctacaaac ccttttactc tgcaacgacc
    10981 cttttcgcta tctgttttag gatcaataat tccgtattta gatgtttcat tttctggtac
    11041 acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa tccatcatca attgttgagt
    11101 caatgggata tcatctttca tcaaatcatc ccccagcatg acgacaaacg gttcatttcc
    11161 tataaatgct tttgcttgaa gcacggcatc ccccagtcct cttggatgag attgacggat
    11221 gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg agtaattctg ttttttcctt
    11281 ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag tgatcttcaa tgggtctttt
    11341 ttcttttccc gttacgatca agatgtcttc gattcccgaa tccaatgctt cttccacaat
    11401 aaactggatc gttggtttat ccacgattgg aagcatttct tttgccatgg cttttgttgc
    11461 cggcaaaaaa cgtgttccta gcccagcggc aggaatgact gcttttttga ccttcactt
//
LOCUS       DAFEHS010000080        11252 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFEHS010000080
VERSION     DAFEHS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.083"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LP327_002310"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LP327_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBL1726402.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LP327_002311"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LP327_002311"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBL1726403.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="LP327_002312"
     CDS             complement(2169..2906)
                     /locus_tag="LP327_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL1726404.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="LP327_002313"
     CDS             complement(2997..3524)
                     /locus_tag="LP327_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726405.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="LP327_002314"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="LP327_002314"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBL1726406.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="LP327_002315"
     CDS             complement(4401..4886)
                     /locus_tag="LP327_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBL1726407.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="LP327_002316"
     CDS             complement(5039..5236)
                     /locus_tag="LP327_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726408.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LP327_002317"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LP327_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBL1726409.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LP327_002318"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LP327_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBL1726410.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="LP327_002319"
     CDS             9211..9423
                     /locus_tag="LP327_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBL1726411.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="LP327_002320"
     CDS             9425..11104
                     /locus_tag="LP327_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL1726412.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3264 a         2428 c         1972 g         3588 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgag catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aa
//
LOCUS       DAFEHS010000081        11115 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFEHS010000081
VERSION     DAFEHS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.084"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(635..1042)
                     /gene="arsC"
                     /locus_tag="LP327_002321"
     CDS             complement(635..1042)
                     /gene="arsC"
                     /locus_tag="LP327_002321"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HBL1726413.1"
                     /translation="MRKIYFLCTGNSCRSQIAEGYGKAILPTDEFEIKSAGIEQHGLN
                     PRAVQVMAEEGIDISTQTSDLIDMDYFNQADLIITLCGDAKDKCPVIPKGIKHVHWDL
                     EDPAKATGTEEEIINKFREIRDDIKSRVANLLD"
     gene            1400..1759
                     /locus_tag="LP327_002322"
     CDS             1400..1759
                     /locus_tag="LP327_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL1726414.1"
                     /translation="MKNEICEISCVHEDKVLLGKEKLQTEDISSLKSVFKILADENRL
                     KIIYALSYEDELCVCDIAATIEATVATTSHHLLSLKKNGIVVSRKVGKLVYYSIKNDK
                     FVQLLNSKLYLEDEVLV"
     gene            1756..3873
                     /gene="cadA"
                     /locus_tag="LP327_002323"
     CDS             1756..3873
                     /gene="cadA"
                     /locus_tag="LP327_002323"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL1726415.1"
                     /translation="MSEKKQVYRVEGLSCTNCAAKFEKNVSQIPKVTDAKVNFGAGKI
                     SIEGEATIAEIEAAGAFENLKVQSEHDTEPRIETKEPFVKRNWNLLVSLFLIILALGS
                     QVVNGEDALLTEGLFILAIIIGGFSLFKEGFSDLLKLNFSMESLMTIAIIGAAIIGEW
                     TEGSIVVILFAISEALERFSMDKARQSIRSLMDIAPKEALIRRNNVEQMINVSKIEVG
                     DIMIIKPGQKIAMDGQVIKGHSSVNQAAITGESVPIEKNINDDIFAGTINEEGALEVK
                     VTKHVNDTTIAKIIHLVEEAQGERAPAQAFVDKFAKYYTPTIMVIAALIVIVPPLFFN
                     GDWNTWLYQGLSLLVVGCPCSLVISTPVSIVSAIGNSAKNGVLVKGGIYLEEIGGLKA
                     IAFDKTGTLTKGTPTVTDFTTVDSKDEEKYFSIITALESYSQHPLASAILKEADNRAI
                     SYKSVVVDEFTSITGKGIQGNIEGITYLVGSPKLFESILTDNSKIIENYQRLQQQGKT
                     AMLLGTDKQILAVIAVADELRESSKAVIEKLHDLGIEHTIMLTGDNATTAQSIGKQTG
                     VTEIKGDLMPQDKLDYIKSLKETYGKVAMVGDGINDAPALAASTVGIAMGGAGTDTAL
                     ETADVALMGDDLQKLPFIVKLSRQTLRIIKQNITFSLGIKLLALLLVVPGWLTLWIAI
                     LADMGATILVTLNGLRLMKVKSK"
     gene            complement(4094..4906)
                     /locus_tag="LP327_002324"
     CDS             complement(4094..4906)
                     /locus_tag="LP327_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726416.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGTQENQYILEMLQKKAGKLDPKIEGLIVDELKQ
                     AGLVSEKEMDRMIEFFIINLIPPTEQDQFTYQCIDEKKQLYLGSTKFGKEAQLYLHHD
                     NQNHILHILPFTFSNFSVDRLLVYLANQESYSYFGKHLPKELVEKAILLYSINQLTVD
                     VRIAPLVYSKETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNVAYPSSK"
     gene            5068..6324
                     /locus_tag="LP327_002325"
     CDS             5068..6324
                     /locus_tag="LP327_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL1726417.1"
                     /translation="MNQVNQHDLGESIRVSREERGWTQRYLAEKVGISRSLLSKVEKG
                     TRQLSAEKLNLILDSLQEEIVPVNRVLIDYLTIHFFSNQHLKLIEAIIGMPIERFEEL
                     DYAPKGYIGQYVWNQVITIRYSIDDTVKGTVMEFSGQGCKHLAMRLKTAKSNWQEFFR
                     KVLDYQGNFTRIDFTLDDFVGSLSIPELKRKVTLGHVWTTFQVSESHGGTDIINNESN
                     GETLYLGSKKSQCRFCFYQKDYEQRKRRGISLEEAEVKNRFELRYRKEKAQSLAKIIS
                     KTHDLTKLFFELLNGAICFYDRAPNDPGAKVDAKWAAFIGNHGATTISLETIPQSFEK
                     SMNWLIHSVSPTLAFIQEVDNHFDSNLINEIISCGELSSRQQKILENLIAEPDYYQEE
                     VEFYIQCLQNMKAAKIHKKSKAQLTH"
     gene            6414..6611
                     /locus_tag="LP327_002326"
     CDS             6414..6611
                     /locus_tag="LP327_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL1726418.1"
                     /translation="MKNTITTYELPYLLTREQASHFLGIDPKSFDKFVRANDELERFM
                     IGRQERYTVTSLINFIKTHSI"
     gene            6717..7766
                     /locus_tag="LP327_002327"
     CDS             6717..7766
                     /locus_tag="LP327_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL1726419.1"
                     /translation="MPSIIKRGNSYRAQISLYNHGQHKKLTKSFSSKKEATRWALENE
                     LEKGNGKQLAERTTTFADFFENWIHIIKKNDVKETTFQNYQRTSQVVKKLFGDIQLKD
                     LNDIVVQRKIDKYAETHSRKTTHEVLLKIKTALRDAYARGYLATDFANLVKTRGKVLD
                     KRNRVLSITELKKLRTYVINHRENEFNILVLLALETGMRRGELLGIRPEDLFEYGIHV
                     RRSLSPTSEDTSLKTKNSRRDISINQEVYDILKSVPIKDNGYFFDPDGFQQSAKLARL
                     LKKLDIPKTTFHGLRDTHASFLFSQDIDIAYVSKRLGHINIQTTQNYYLELMPEKKHQ
                     QDADALNLLNSLSNL"
     gene            complement(8064..8684)
                     /locus_tag="LP327_002328"
     CDS             complement(8064..8684)
                     /locus_tag="LP327_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726420.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(8765..9175)
                     /locus_tag="LP327_002329"
     CDS             complement(8765..9175)
                     /locus_tag="LP327_002329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726421.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            9205..10383
                     /locus_tag="LP327_002330"
                     /pseudo
     CDS             9205..10383
                     /locus_tag="LP327_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT         3699 a         1935 c         1954 g         3527 t
ORIGIN      
        1 ttaggattaa ggtaactgcg ctcagcattg cttcgcccag tacgattctc gtaactcgtg
       61 aactcgaacg ataatcgcaa ttaacgtttt gagatgtgac tcgtacttat tacaagtact
      121 tctatcaata gaaatccaat tatatcaaca ttacaaacat ctaaaaaaca gtttgatatg
      181 gataaaatag gattaatatt gatattactt gaaccaattt gaaccaaaga tgaatggtag
      241 ttgaatacaa ttttcttact agtttatcaa tcttttttag agttattcct cccctcttat
      301 ttatttgaga ccgtattgat ggctggatag ctgtctaata ggggtttcaa ggccagaatt
      361 agctaagcta ctagattctc gaataattgt ataagccggt cacacctact taaatgtgat
      421 tgagaaggtc aaatataggg tcaaaaaata ctcgtcagta tgtcagatag ataaatccaa
      481 ttttacgcca aaagcagatc cttttctctt aaaatagtaa gagccatcga agatttatgt
      541 ttcacttcgc acggctttta ctttaatttt ccaatttatc aaaaggacat tttacgtctt
      601 tttatctttt accgagatag atgacagttg ctacttaatc tagcaaattc gctacacgac
      661 tttttatatc atctctaatt tctctgaatt tattaatgat ttcttcttct gttccagttg
      721 ctttggctgg atcttccaaa tcccaatgaa catgcttaat cccttttgga attactggac
      781 atttatcttt ggcatcgcca catagcgtaa taattaggtc tgcttgatta aaataatcca
      841 tatcgatcaa atcagatgtt tgcgtagaga tatctatccc ttcctcagcc attacttgaa
      901 cggctctagg atttaaccca tgttgttcaa tgccggcgct tttaatttca aattcatctg
      961 ttggcaaaat cgctttccca tagccttctg caatttgact acgacatgaa ttgcctgtac
     1021 ataaaaagta aatttttctc attattattt cctccgatat cagacaattc tgatggattt
     1081 tgaatgaacc aatttttttg tgcgattaga tattttaact aacagtaaca tgactggcac
     1141 ttcaactaag atgccaactg ccggcgctta tgtaaaaata tagataatca tctatgtttt
     1201 gtcaaatgct tctgtctttc agttcaaatt aattagttta ttatttgcat tcgattcaat
     1261 ctaatttgaa aatcactact tatatagatg catccataca atctatataa ctcctcacat
     1321 attcaaatta aaatttgatt attctattaa tctatgatac actacattca aatatccatt
     1381 agaataaagg aggagctgta tgaaaaacga aatttgtgaa atctcatgtg ttcatgaaga
     1441 taaagtttta ctaggaaaag aaaagttaca aactgaggat atttcgagct taaaaagtgt
     1501 cttcaaaatt ttagctgatg agaatcgctt aaaaattatc tacgccctaa gttatgagga
     1561 tgaattatgt gtatgtgata tcgcagcaac tatcgaggca accgtggcaa caacctcaca
     1621 ccatttatta tcattgaaaa aaaatggaat tgttgtgagt cggaaagtag ggaaattggt
     1681 ttattactct atcaagaatg ataaatttgt ccaactacta aattccaaac tgtatttaga
     1741 agatgaggtg ctagtatgag tgaaaaaaaa caagtctatc gggttgaagg attgagctgt
     1801 acgaattgtg cggcaaaatt cgaaaaaaat gtttctcaaa taccaaaagt taccgatgca
     1861 aaagtaaatt tcggtgccgg aaaaatctct atcgaaggcg aagcgactat tgcggaaatt
     1921 gaagctgcag gagcttttga gaatcttaaa gttcagtccg aacatgatac agaacctcgc
     1981 attgaaacaa aagagccttt tgtgaaaaga aactggaacc tattggtttc actgttttta
     2041 attattttgg cattaggatc tcaagtagtt aacggtgaag atgcgctatt aacagaagga
     2101 ttatttatac tagcgattat tatcggcgga tttagtttgt ttaaagaagg attttcagac
     2161 ctattaaaat tgaatttctc aatggaatct cttatgacaa ttgcaatcat tggtgctgca
     2221 attattggcg agtggactga gggctcaatc gttgttatct tatttgcaat tagcgaagct
     2281 ttagaacggt tttcaatgga taaagcaaga caatcaatac gttccctaat ggatattgct
     2341 ccgaaagaag cattaattag aagaaataat gttgaacaaa tgattaatgt ttcaaaaatc
     2401 gaagtaggcg atattatgat cattaagcct ggacaaaaaa ttgctatgga tggtcaagtc
     2461 attaaaggcc attcctcagt aaatcaggct gcgattaccg gtgaatctgt ccctattgaa
     2521 aaaaatatta atgatgatat ctttgcagga acgattaatg aagaaggtgc acttgaagtc
     2581 aaagttacaa agcatgtgaa cgacacgaca attgccaaaa ttattcacct agttgaagaa
     2641 gcacaaggtg aacgagcacc tgctcaagca tttgtcgata aatttgcgaa atattataca
     2701 cccaccatta tggtgattgc cgcattaatc gtcatcgttc ccccattatt ttttaacgga
     2761 gattggaata cttggttgta tcaagggctc tctttattag ttgttggctg tccttgttct
     2821 ttagtaatct caacacctgt gtctatcgtt tcagcaattg gaaactctgc taaaaatggt
     2881 gttttggtaa aaggcggtat ttatcttgaa gaaattggtg gcctcaaggc gattgccttt
     2941 gacaaaacag gaacattgac taaaggaaca ccaacggtaa cagatttcac tacagttgat
     3001 tcaaaagacg aagagaaata tttctcaatt attacagcat tagaatcata ttctcaacac
     3061 cctctcgcgt cagcaatctt aaaagaagct gataatagag cgatttctta taaatcagta
     3121 gttgttgacg agtttacttc aattactgga aaaggaatac aaggaaacat tgaggggatt
     3181 acctatcttg tagggagtcc aaagcttttt gaatctatct taacagataa ctcaaagatt
     3241 attgaaaatt accaaagact tcagcaacaa ggaaaaacag caatgctctt aggaacagat
     3301 aaacaaattt tagcagtcat tgcagtagct gatgagttaa gagaatcaag taaagcagtc
     3361 attgaaaaat tacatgattt aggaatcgag catacgataa tgctgactgg tgataatgct
     3421 acaactgctc agtcaatcgg aaaacaaaca ggtgtaactg aaataaaagg cgatttgatg
     3481 cctcaagata aattagatta tattaaatca cttaaggaaa cctatggaaa agtcgccatg
     3541 gttggtgatg gtattaatga cgcaccagca ttagctgcct caacagtcgg tatcgcaatg
     3601 ggtggcgctg gaacagatac agctttagaa acagctgatg tagctttaat gggtgatgac
     3661 ttacagaaac ttccctttat agttaaatta agtcgacaaa cattgagaat cattaagcaa
     3721 aatattacct tctcattggg aatcaaacta ttagcattat tacttgtagt tccaggctgg
     3781 ttaacgttat ggattgctat cttagctgat atgggagcaa ctatattagt taccttgaac
     3841 ggcttacgac tgatgaaagt taaatctaaa tagacaagta gtagcataaa aagaggaaag
     3901 actagcgacg gcagcgccgt ataagctagt ctttcctctt tttaatgctg ttgcaggacg
     3961 gcgtagccgg actgcatggg caagcccctc tttctaacag ggggctaaca aagaacagga
     4021 aactgtgaca actacccatt tgggagtgtc ctagcagtgt tttctttgtc acatgcttat
     4081 caatcttgtc acctcatttt gatgatggat acgctacgtt taacagacgt tctttctcga
     4141 cttccatcac gaatggtaga taaagatatt gttgttctag tagatattga ttgtcctctg
     4201 tgtactgata agaagctgtt tcttttgaat agaccaacgg ggcaattcgc acatcgacgg
     4261 tcaattgatt gatcgagtat agaagaatgg ctttttcaac tagttccttg ggcaaatgtt
     4321 tcccaaaata ggaataggat tcttgatttg ctaaatagac gagtaagcga tccacactaa
     4381 agttggaaaa ggtaaagggc agaatgtgca gaatgtgatt ttgattatca tggtgtaaat
     4441 agagttgagc ttctttacca aatttagttg aaccaaggta aagctgcttt ttctcatcga
     4501 tacattgata agtaaattgg tcttgttccg ttggtggaat gaggtttata ataaaaaatt
     4561 caatcatccg atccatttct ttttcactaa caagaccggc ttgtttcagt tcgtctacta
     4621 ttagcccttc aatcttagga tcaagcttac ctgccttctt ttgaagcatt tctaaaatgt
     4681 attggttttc ttgagtacca tacaaaagat attcgttggt gacatggcct atttctgcaa
     4741 tttggtgaag ggtttcggtt tttggaaggt taatcccacg ttcccagtta ttgactgtac
     4801 ttcgtggtaa atcaccaatt agtttgccaa atttctccat actcaacttt aactcttgac
     4861 gaatctgccg aattcgattg cccactgctt gcttgtctat ctccatgaaa actcctcctc
     4921 aggataagtt cttaaattca gtatacgctt gtacttaaaa aaagtcaaaa tgtattgact
     4981 taaaataaat acaggcgtac aatcgatttg tacttaaaat aagtctgatg gttctttgac
     5041 aatttaatag cagaaggagg tttttctatg aaccaagtca atcaacatga tctcggggaa
     5101 tcgattcgag tttctcgaga agaaaggggg tggacccagc gatatttagc agaaaaagtg
     5161 gggatttcga gatcgttatt atcgaaagta gaaaaaggaa cgagacaatt aagtgcagaa
     5221 aaattgaatc ttatactgga tagtttgcaa gaagagatcg ttccagttaa tcgtgttttg
     5281 attgattatc taactattca ctttttctcg aatcaacatt taaagttgat tgaagcaatt
     5341 attggcatgc ctattgaacg ctttgaggaa ctggattatg cgccaaaggg ctatattggg
     5401 caatatgttt ggaatcaagt aatcacgatt cggtattcca ttgacgatac ggtaaaagga
     5461 accgtaatgg agttttcagg tcaaggctgt aaacatcttg ccatgcgttt gaagaccgca
     5521 aaatcaaact ggcaagagtt cttccgtaag gtattagatt atcagggaaa ctttacacgg
     5581 attgatttta ctttagatga ttttgtgggg agtctcagca ttccagaact gaagcgtaag
     5641 gtaacactag gccatgtgtg gaccactttt caagtgagtg aatctcatgg cggtacggat
     5701 atcataaaca atgaatcaaa tggtgagacc ttgtatttag gctcaaagaa gagtcagtgt
     5761 cgcttttgct tttatcagaa ggattatgaa caacgaaagc gacgaggaat ttctttagaa
     5821 gaagcagaag taaaaaatcg ctttgaacta cggtatcgaa aagagaaagc gcaaagcttg
     5881 gcaaagatta tttcaaaaac ccatgattta accaaactat tctttgagtt acttaatgga
     5941 gcaatttgtt tttatgaccg tgctccaaat gatccaggtg caaaagtcga tgccaaatgg
     6001 gcagccttta ttggcaatca tggcgcaacc accatttctt tagaaacaat tcctcaaagc
     6061 tttgagaaaa gtatgaattg gttaattcac agcgtttctc ccaccctcgc atttattcaa
     6121 gaggtagata atcattttga ttcaaatttg attaacgaga ttattagctg tggggaactt
     6181 agttcaaggc agcaaaaaat attagaaaat ttgattgctg agcctgatta ttatcaggaa
     6241 gaagtagaat tttatatcca gtgtcttcaa aatatgaagg cagcaaaaat acacaaaaaa
     6301 agcaaagcac agcttacaca ttaaaatgta taaacgtact ccgccctaga caagttgagt
     6361 atagcacatt tccttgtgtc agccaaaaaa catttttgga aaggaaataa actatgaaaa
     6421 atacaataac tacttatgaa ttgccctatc tacttactag agaacaagct tctcattttt
     6481 tagggatcga tccaaaatct tttgataaat tcgttcgcgc aaacgatgag ttagagcgtt
     6541 ttatgatagg aagacaagaa cgatacactg tcacttccct cattaatttt attaagacgc
     6601 actctatcta gctaaaatat cgtaggatgg ttgattagat gcttttcgcc atgtagaatg
     6661 acggtgtatt caactagcat ttatcaacca tctttcagtg aaaggatttg ttataaatgc
     6721 ctagtataat taaacgtggt aactcatata gagctcagat ctcactttac aatcatggtc
     6781 aacataagaa attgacaaaa tcatttagct ctaaaaagga agcaactcgt tgggccttag
     6841 aaaatgaatt ggagaaagga aatggaaaac aattagcaga gcgtacaaca acatttgctg
     6901 acttctttga aaactggatt catatcataa agaaaaacga tgtaaaagaa acgacttttc
     6961 aaaattatca aaggacttct caagtggtga agaaattatt tggcgatata cagttaaagg
     7021 atttgaacga tattgtagta caacgaaaaa tagataagta tgcagaaact cattctcgta
     7081 aaaccactca tgaagtacta ttgaaaatca aaacggcatt acgtgatgct tatgctcgtg
     7141 gctatcttgc taccgatttt gcaaatttag tcaaaacacg agggaaagtt ttagataaga
     7201 gaaaccgtgt actttctatt accgaattaa agaaactgcg tacttatgtc attaatcatc
     7261 gagaaaatga gtttaacatt cttgtactcc ttgccttaga aactggcatg agacgcggag
     7321 agcttttagg tatacgtccc gaagacctct ttgaatatgg tatccatgtc agacgttccc
     7381 ttagtccaac ttctgaagat acctcgttaa aaacaaaaaa ttcaagacga gatatttcca
     7441 ttaatcaaga agtctacgat attctaaaat ctgtcccgat taaagataat ggatattttt
     7501 ttgatcctga cggctttcaa caatctgcta agctcgccag attactgaaa aaactagata
     7561 ttccaaaaac taccttccac ggcctaagag atactcatgc ttcttttcta ttttcacaag
     7621 atattgatat tgcctatgta tcaaaacgct taggtcatat caatattcaa acaacgcaga
     7681 actattatct tgaattgatg ccagaaaaaa agcaccagca agatgctgat gctttaaacc
     7741 tcttaaattc tttgtcaaat ttataatttg aaccaacttg aaccaaaaaa ttcttgtaaa
     7801 cgttgataca ataaggaatt gattaacgtt ttgagaactg tgaagcttta cgggctttct
     7861 taagacctgg ttaaacaaaa ctctatttta ttgcatttta caggaatgta tttcatgcga
     7921 aattaataag tctttgttta tagagtttta ctagggtata aaaagttgta tgttatcatt
     7981 acctcaaaac attacctcag cttggcgagt tggttgattg aagaataaat catttatcta
     8041 gatatctcaa taaaaaatga tttctaagaa acttgtattt tcgtgttatt atttttttgc
     8101 cctatcaaaa tttctaattc ttctagcgta atatgattat gacacataat cgattcaatt
     8161 acttcaatac ctattttttt cgagaaagat cccttttgtt cactcacaaa aatactttca
     8221 gaagacatat ataaggaacg aagttttgga attagtatat cgtacaaata gaccaccata
     8281 aaatcgtcct tgcaaccatt ctcaaaaaga attaaatttg ctgattgtgt tacatatatc
     8341 actgaattac attcatggtc aattccaaaa acaatgtcta cttcattttc ttttctaatt
     8401 cgcgcagtta catttttcaa actttcacca ttacttataa atgctttagt tactgtaagc
     8461 tctgatgttt gttcagatat tccttttata atattttgtt catcagccac attccccata
     8521 aatccgccga ttccttttaa gtaataattc tcacctaaga gtcgcttgca ttcactgaat
     8581 tgataaaaaa accagttaaa aaaatcttct ggaaaaataa attctgatcg aactttattt
     8641 atatttttag ctcctattaa ttttataact cgattaatgt tcacttgctg cgatgaacat
     8701 ataactgaat aaattgagtt gtttaattca aaaaaacaac tttaacatat gcatcattaa
     8761 ttcgttattt tttaggtaga tattgatcat tgttatctct ctttttttgc ttcccatact
     8821 ccaacttaga aattgccgtt ataaaattta ttttaaaggt gactttttcc cctgaagtat
     8881 cggtagtagg aatacttaat acctcagctt ctgaggaggt atcccaatta tagttatact
     8941 ttgcatcatc accataaaga acaattccat tttttttatc ttgtgccgtt agctttactg
     9001 gttcgcttac ttttcttgtt aatatcttgg actttacatc atcgatattg cttattttat
     9061 caagcttaaa taatttacaa attaatttcg tactagtttt tatatttgac gagagtgtaa
     9121 aatattttgt gtaaatagaa aaaaggaagt cccttctgta gaatagagtt accacaacac
     9181 attcacagaa aagaggactt ccatatgaac gattttacta cagaaattct aaagactcta
     9241 gcgaacaaag gcgatttgaa tgaattattc cgtgtccatt tggaaaaagc tgtcaatacg
     9301 cttctcaaaa cggagttaac ggctttcctc gattacgaaa agtacgatcg cattggtttt
     9361 aacacgggga attctcgtaa cggctcctat gaccgtacgg tcaagaccga gtacggggaa
     9421 cttcatctcc agattccgcg cgaccgcaac ggcgagttca agcaacagac tgttcctgct
     9481 tatagacgga cgaatgatac cttagaggag accgtcattc acctcttccg aaaaggtatt
     9541 accatgtcgg aaatcgcaga cttgattgag aaaatgtatg ggcatcacta cacgccccaa
     9601 accatgtcca acatgactaa agtgctgact gaagaagtaa atgcatttaa agttagaact
     9661 ctaaatgata agtatgtcgc tattttcatg gatgctactt atattccttt aaaacggcaa
     9721 accgtctcca aagaagcaat ctatattgcc attggtatac gagaagacgg cactaaagaa
     9781 gtactaagtt atgcgattgc tccgactgaa tcgacctacg tttggaatga gttgttacag
     9841 gacatttact ccagaggggt tcatgatgtc ttactgttta ttaccgatgg cttaaaaggc
     9901 atgaaagata cgattcacca gatttatcct aaagctaaat accaacattg ctgtgttcac
     9961 gtttcccgta atattgctca taaagtacgt gtcaaagatc gaaaagaaat ctgtgatgat
    10021 tttaaggctg tttatcaagc tagctcaaag gaagaggcaa ataccttttt agggagtatg
    10081 attgagaagt agcaaaaaac ttatcctaaa gtgacgcagt cactgataaa aaatcaagat
    10141 ttattgactt tttatgagtt tccgcctggt atccgccgaa gcatttactc aactaattta
    10201 atcgaatctt tcaagaaaca aatcaagaaa tacagccgca gaaaagagca gtttcaaaat
    10261 gaagaatcac tagagcgttt cctagtatcc atctttgata catacaatca aaaattctta
    10321 aatagaagcc ataaaggctt ccaacaagtg acggatacat tagcttcaat gtttactgag
    10381 taacccatta ttttgcagaa ggacgagtta tttacacaaa attattgacg ctccctccaa
    10441 attacttcat caaaacattg atataatttt tattatatca gtatttattc ttgatacata
    10501 aatgagaaaa taaatacttt aaatctattg actacaagta gtcattaagt cttcataaga
    10561 tgtacaatat gacattaaaa aaataaaagc ttacgaccat ccgttaaatc tgacactaaa
    10621 taatggactg ctttcttttg tattagcagg ttacgttgac tatgaatatt caccagatta
    10681 tttggagaaa cctcttcaac acgagaacaa tgatattaat tttagatttg tctacgagtc
    10741 aaaaagtaaa acatggttag ttgatcaatt aagcacgata aacacaagct tccatgaatt
    10801 acctaaacct gaaaatgaag ttgagatggt cattaaaatc ttctaaaaaa gaagcgttga
    10861 aggggaaata ttcctttcaa cgctcttttt tatatgctta gaaacataaa taaattgtat
    10921 actttctttt tagcccctat tacttttcac gaaaaatatc tttttttcga cgctagcgtt
    10981 tctctacttc taatactgta cacagttttt gtattattga aaaattactc attatttagc
    11041 attaatccac atcgttttat caatcgacct tcatactaaa ctactttaaa gtcatcttcc
    11101 ttctcgtttt tcatt
//
LOCUS       DAFEHS010000082        10606 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFEHS010000082
VERSION     DAFEHS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.085"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="LP327_002331"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="LP327_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBL1726422.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="LP327_002332"
     CDS             808..1242
                     /locus_tag="LP327_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBL1726423.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="LP327_002333"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="LP327_002333"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBL1726424.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3469..5919
                     /gene="parC"
                     /locus_tag="LP327_002334"
     CDS             3469..5919
                     /gene="parC"
                     /locus_tag="LP327_002334"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBL1726425.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6196..8427
                     /gene="pflB"
                     /locus_tag="LP327_002335"
     CDS             6196..8427
                     /gene="pflB"
                     /locus_tag="LP327_002335"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBL1726426.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8671..9432
                     /gene="pflA"
                     /locus_tag="LP327_002336"
     CDS             8671..9432
                     /gene="pflA"
                     /locus_tag="LP327_002336"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBL1726427.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9517..10443
                     /locus_tag="LP327_002337"
     CDS             9517..10443
                     /locus_tag="LP327_002337"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBL1726428.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
BASE COUNT         3755 a         1833 c         2277 g         2741 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtcg gtcacctgat ggaaaatcga
     3481 caagaagtac aagaattaac tttagaagaa gtgatgggtg accgcttcgg acgttattcc
     3541 aaatatatta tccaagaacg tgcactgcct gatatccgtg atggattgaa gccagtgcaa
     3601 cgccggatat tatttgccat gaacaaagat ggcaatacgt atgacaaagg attccgtaaa
     3661 tcagcaaaat ctgtcggaaa tatcatgggg aattatcatc ctcatggtga ccgcagtatt
     3721 tatgaagcga tggtccgcat gagccaagac tggaagttgc gggaagtact gatcgaaatg
     3781 cacgggaata acgggagtat ggacggtgat ccacctgctg cgatgcgtta tactgaagcc
     3841 agattatcca agttaagtgg agagatgcta gcagatatcg agaaagaaac agtcgatctc
     3901 gtgtggaatt ttgatgatac agaaaaagaa ccaacagtcc ttccagctcg atacccaaac
     3961 cttctcgtca atggatcgac aggtatatct gctggatatg caacagaaat cccgactcat
     4021 aacttagctg aagtgatcga tggcacaatc tatatgatcg atcatccaca agcgagcctt
     4081 gaaaaattga tggaatatat tcctggacct gactttccaa caggagggat cttgcaagga
     4141 aaagatgaaa tcaaaaaagc ttatgaaaca ggacgaggaa aagttattct tcgttcgaaa
     4201 acaaagatag aggcaatgaa gggtaataaa cagcaaatcg tgatcacaga aatcccatat
     4261 gaagtaaata aagcgacctt ggtgaaaaaa atggatgaga tccgtttgaa taagaaaatc
     4321 gatggaatag ctgaagtacg agatgaaagt gatagaacag gtctgcaaat tgtcgtcgaa
     4381 ttgaaaaaag acgccaatgc tcaaggaatt ttgaattatt tgttcaaaaa cactgagcta
     4441 cagatcaatt acaatttcaa tatggtcgct atcgaccata tgactccgca tcaagttgga
     4501 ctgaaagaca ttttaagaag ttatatcgag catcgaaagc aagtcataac aaaacgtagt
     4561 caattcgatt tagccaaagc tcaaaaaaga cagcatatcg tagaaggatt gatgaaagct
     4621 ttatcgattc tggatgaagt aatcgcaacg attcgtgaaa gtaaagacaa aaaagacgcc
     4681 aaaaagaatt tagtcgacgt ctttcaattt acagaagaac aagcagaagc aattgtcaca
     4741 ttgcaattat accgtttgac gaacacggat atcacagagc ttcaaaagga atccgaaagc
     4801 ttgattgcgc aaatcacaga gttgaataaa atcctttcaa atgacaaaga gcttttctct
     4861 gttatgaaaa aagaattgcg agaagtgaaa aaaaattatt cttccgctcg tctaacgaca
     4921 atcgaagacg aaatcgaaga aatcaaaatt gatacgcaag tcttagtggc acaagaagac
     4981 gtaattgtca gtgtgacgcg agaaggctat gtgaagcgaa caagccttcg ttcttatagt
     5041 gcttccaaac cagaagaaat cgggatgcga gaaggcgact acttgctata ctcaggtgaa
     5101 ttgagtacat tggaccatgt tcttctgatc acaaataaag caaatgtgat ctaccgccct
     5161 gtccatgaac tacccgattt gaaatggaaa gatgctggtg agcacatttc tcaaacaatc
     5221 tccaatctgt ctgtagacga atcgatttta gctgttttcc catatcaaaa aattcaagca
     5281 gagaaaacat ttgtattcat cagcaagaat ggattgatca agcaaacacg aatgacagag
     5341 ttcgaaccat ggcgcaccta taagagccgt ccgctcagct gtatgaagct aaaggcggaa
     5401 gaagatgaac tgattgctgt gtatctgaaa gaagatcagc caggcttaga tgttttctta
     5461 gtaacgcatc aaggaatggg tttgcgctat cctttaacag atgttcccgt tgttggagca
     5521 aaggcagctg gagtgaagtc tatgaatttg aaagatggag ataggattat caacgggctg
     5581 cttgtgttag cagaaggtga tacgccgatc gtaatcgtta ctcagcgagg agctgttaaa
     5641 cgcatgcttg ctcaagaaat cagccaaact tcacgtgcga agcggggagt gacggtcatg
     5701 cgtgagctga aaaaaaatcc gcaccgtatt atctatatgt cggaaggaag atcaaaagaa
     5761 ataaccttga tgaatcaaaa agggcagcag ttaacgattg acccaactga tttccctata
     5821 ggtgatcgaa catcgaatgg atcatttgcg atggatgaga aaaaaggtgg agaagtcatc
     5881 gaagttatcg atgctcctga gattttcatc gagtcgtaac tgttgtagta ttttaatgaa
     5941 gagacgccat ctgtattaat acaggtggcg tctctttttc attcattttt aataaatcct
     6001 tatacgtttg caaaaaaaga tgaaagtgtt cacaatgctt aaatgttggt atgtcaacgt
     6061 tgatttaaat atatgtaaat aaaacataaa aattgttaaa taaaaaacaa gaaaaccctt
     6121 gcaaagatag ggtgctgtgt tatactaatc acgtaaaaac tgttacataa aacagaaatt
     6181 taggaggtcc atacaatgga gcaatggaat ggatttaaag gtaccaaatg gaaagaaggc
     6241 gtagacacta gagactttat tcaaaataac tacactgaat atcgcggtga tgacagcttt
     6301 ttggaaggcc caacagaagc aacagaaaaa ttatgggatg aattgcaaaa gttaaatgat
     6361 atccaattcg agcgtaacgg tgtctatgat atggacacag aaatcgtatc aactattact
     6421 tcacacgatg caggctattt ggataaagat ttggaaaaaa tcgtcggtct acagacagac
     6481 aaacctttga aacaagcatt catgccgttc ggcggaatca atatggctaa caatgcgcta
     6541 aaaagcaatg gctatacacc aaacgatgaa ttgacacata ttttcactga ctggcgcaaa
     6601 acacataatc aaggtgtatt tgacgcttat actgctgaaa tgcgtgctgc tcgtaaaaac
     6661 aaaatcatta cagggcttcc agacgcttac ggtcgcggac gtatcatcgg tgactatcgt
     6721 cgtgtggcat tgtatggaat cgactatttg atggaacaaa agaaaaaaga tcatgatatg
     6781 actggttatc atacaatgag tgaagaagtg attcgtttac gtgaagaaat cactgaacaa
     6841 tatcgtgcat tagcgcaaat gaaagaaatg gctgcgaaat atggctatga tatttctaaa
     6901 ccagctgcaa atgcaaaaga agctgtccaa tggctatact tcggatattt agcagcaatc
     6961 aaatcacaaa atggtgctgc gatgtcaatc ggacgtatct cagcattcct tgatatctat
     7021 atccaacgtg atttggataa tggagtaatc acagaaaaag aagcacaaga attgatcgac
     7081 cacttagtaa tgaaattacg tatggtcaaa ttcgcgcgta cacctgaata taaccaatta
     7141 ttctcaggat tccctatctg ggctacttta tccattgcag gtatgggatt agatggtcgt
     7201 tcattagtta caaagaatga tttccgtctg cttcatacat taacaaatat gggaccttca
     7261 ccagaaccaa acttgactgt tttgtattct gaacaccttc cagaaggatt cagaacatat
     7321 gctagcagaa tcgctatcga aagttcttcg attcaattcg aaaatgataa tcagcttcgt
     7381 gaacaatggg gttctgatga ttgtgcgatt gcttgctgtg tatcaggaac agttgtcgga
     7441 aaagacatgc aattctttgg cgctcgtgct aacttggcta aaacactatt atatgccatc
     7501 aatggcggag tcgatgaagt gactggcgcc caagttggac cagaatatcg tccaatcaca
     7561 tcagaaatac tagactttga agaagtgaaa cataacttca tgaatatgat ggattggtta
     7621 gcagaactat atgtcaatac gttgaacatc attcattata tgcatgataa atattcttat
     7681 gaagcagctc agctggcttt gatggattct gacttgcaac gcacatttgc tacaggtatt
     7741 gctgggatct ctcatgcggc agacagcttg tcagcgatca aatatgcaaa agtaaaagca
     7801 atccgtaatg aaaaaggaat agcagttgat tttgaagtgg atggcgactt ccctaaatac
     7861 ggaaatgatg atgaccgtgc agatgaatta gtttcatgga tcttggaata cttcatggga
     7921 caaatcaaac gtcataaaac ttaccgcaac tcaaaaccaa caacttcatt attgacgatt
     7981 acttcaaatg ttgtttacgg taaaaataca ggaaatactc ctgatggccg tgaagctgga
     8041 aaaccacttg cgccaggagc aaatccaagt tacaatgcgg aacaaagtgg gttattagct
     8101 agcctgaact caacagctaa aatgaattac ggctgtgcat tggatggaat ttcaaatacc
     8161 caaacaatca atccttcagg tcttggaaaa gaacaagata caagaatcga aaacttgcgt
     8221 aatgtcttag atggatactt ctcaaaagga gcttatcatt tgaatgtcaa tgtattcagt
     8281 aatgatttat tgcgtgatgc aatggaaaat ccaatgaaat atccgaactt gacgatccgg
     8341 gtatctggtt atgcagttaa attccgtgac ttgacaagag aacaacaatt agatgtcttg
     8401 atgagaactt cacacgacag aatgtaaaaa gttcgtgaaa aaagtttttc gacttagcgt
     8461 cgaaaggacg ttgaatagcc atcgcatcaa aatcgaataa tcagtatgct tatttgaaag
     8521 aaacgattta caggggcagg acttgtatgt tccgtccctg taaattttac acataacgaa
     8581 gcggcaattg cgaaaaattt tgttcagaaa gcttcgcttc gacagctaac ttgtacttaa
     8641 aatgaaatca aagaaaagat gtgaacttaa atggaagaaa aaacaatcgg ctatgttcat
     8701 tctatcgaaa ctttcggttc agtagacgga cctggactgc gctttgtcgt gtttatgcaa
     8761 ggatgccgca tgcgttgcca attttgtcac aatccagata cttggaatat tggcggaggg
     8821 aaagaataca cagcagatga acttttagat aaagcagaac gcttcagacc ttactgggga
     8881 gataaaggtg gtattactgt tagtggtgga gaacctctat tgcagatcga ttttttgatc
     8941 gaactattta aaaaagcaaa agagcggaag atgcatacta cattggatac atgcggaaaa
     9001 ccttttacgt atgaagaacc tttcttttca cgttttcaag aattgatgaa gtatacagat
     9061 ttactgttat ttgacatcaa acacatcgat aatgaagagc ataaaaaact aacgcattgg
     9121 gacaatgaaa atattttaga aatggctcaa tatttgtcaa aaatcaacaa accggtttgg
     9181 attcgccatg tactagttcc tgagcgaagt gattatgatg aatatttgat ccgactagac
     9241 aactttattc aaaccttgtc aaatgtagac agagtagaga ttcttcctta tcatacgatg
     9301 ggaaaataca agtgggaaac acttggtttg aaatatccat tagaagggat cgaaccacct
     9361 acaaaagaac gtgtcgaaaa tgctaaacga ttgcttcata cagaagagta tacaaaatat
     9421 ttaacgcgat aggtcgcatc ggaaagaaga taagtattgc ttctttccgt ttttttgcta
     9481 ttattaaata ggagaaatca caaaggagcg aatgacatgt caaaaatttt agtttttggt
     9541 caccaaaatc cagatacaga tgcaatcggg gcagccattg cgttttcaca cttgcaaaat
     9601 gaattaggta aagaaacaga agcagttgct ctaggtacac ctaatgaaga aacacaatac
     9661 gcattagacc actttcagct atctgcacca cgagtagtag agtcagctaa agcagaaaat
     9721 gcagatgtca tgcttgtgga tcataatgaa ttccaacaaa gtatttcaga tatcagcgaa
     9781 gtaaatattt tagctgtagt agaccaccat cgtattgcaa actttgaaac agctaatcca
     9841 ttgtactatc gtgcagaacc agttggatgt acaagtacga ttattttaaa actatataaa
     9901 gaaaaccaag taacaattcc taaagaaatc gcaggaatca tgctctcagc tatcatttca
     9961 gatacattgt tgttcaaatc accaacatgt acgcctgaag atgaagcagc tgcaaaagaa
    10021 cttgcagcaa tcgctgaagt cgatctaaac acttatggac ttgaaatgtt gaaagcagga
    10081 acaaatctaa gtgataaatc agctgcggtt cttcttgatt tggatgcaaa aagtttccca
    10141 atgggcagtt ccaacgttcg tatcgctcaa gtcaatacag tggaccttaa tgaagtgatg
    10201 gatcgacaag cagagctaga agcagcaatg gaagatgaaa tgaaagcaaa tggctacgat
    10261 ctgttcgtat tagttgtcac aaacatctta gacagtgatt cagaattact tgttgctggt
    10321 aaaggaaaag aaaaagtaga agaagctttc caaacaactt tgaccaacaa ccgtgctttg
    10381 cttaaaggtg tcgtttcacg taaaaaacaa gtcgtacctc aattgacggc tgtatttagc
    10441 taatcaaatg gtagtatgaa gcattcaaaa tgaatggata agcgattgat ataaaaagaa
    10501 acatagaaca tactaaaggc cgaagaaatg gaggagaata acagtcaatt atgactcttg
    10561 ttcggctctt tgtcaaatga tgttggtaaa gagaaatctt agaata
//
LOCUS       DAFEHS010000083        10493 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFEHS010000083
VERSION     DAFEHS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.086"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..357)
                     /locus_tag="LP327_002338"
     CDS             complement(<1..357)
                     /locus_tag="LP327_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL1726429.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
     gene            complement(709..1068)
                     /gene="rplT"
                     /locus_tag="LP327_002339"
     CDS             complement(709..1068)
                     /gene="rplT"
                     /locus_tag="LP327_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBL1726430.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="LP327_002340"
     CDS             complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="LP327_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBL1726431.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1393..1917)
                     /gene="infC"
                     /locus_tag="LP327_002341"
     CDS             complement(1393..1917)
                     /gene="infC"
                     /locus_tag="LP327_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBL1726432.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1961..2086)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2237..2869
                     /locus_tag="LP327_002342"
     CDS             2237..2869
                     /locus_tag="LP327_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL1726433.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2871..3776)
                     /locus_tag="LP327_002343"
     CDS             complement(2871..3776)
                     /locus_tag="LP327_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL1726434.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3861..4520)
                     /locus_tag="LP327_002344"
     CDS             complement(3861..4520)
                     /locus_tag="LP327_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL1726435.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4836..5780
                     /gene="mvk"
                     /locus_tag="LP327_002345"
     CDS             4836..5780
                     /gene="mvk"
                     /locus_tag="LP327_002345"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBL1726436.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5773..6750
                     /gene="mvaD"
                     /locus_tag="LP327_002346"
     CDS             5773..6750
                     /gene="mvaD"
                     /locus_tag="LP327_002346"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBL1726437.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6763..7848
                     /locus_tag="LP327_002347"
     CDS             6763..7848
                     /locus_tag="LP327_002347"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBL1726438.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7845..8909
                     /locus_tag="LP327_002348"
     CDS             7845..8909
                     /locus_tag="LP327_002348"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBL1726439.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9158..9961)
                     /locus_tag="LP327_002349"
     CDS             complement(9158..9961)
                     /locus_tag="LP327_002349"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBL1726440.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="LP327_002350"
                     /pseudo
     CDS             complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="LP327_002350"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10268..>10493)
                     /locus_tag="LP327_002351"
     CDS             complement(10268..>10493)
                     /locus_tag="LP327_002351"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBL1726441.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3104 a         2220 c         2008 g         3161 t
ORIGIN      
        1 acctaatgac atcaagatcg gcaacacaat aactgcaata gaaatcactg gtccgattcc
       61 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
      121 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
      181 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
      241 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
      301 cgagtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat
      361 gacaaaagcc gaccatcaag ttgtagcctt ttacacagta ccagaccatg aaaaagaatg
      421 agataactaa caaaatacca ataataatat cactcatgtt attcctccgt ccattttgtt
      481 tttctgataa gcaaactaat atatcagtca aactcattct aacttatgta gttagcgctt
      541 tcattaaagg atgaccataa atgttcagtt ttcaattaaa aaaccttaat tatgttattt
      601 tagacttata cgttattgtc ctgtttcatt tgatggagga ttgtaaataa aaaaagagga
      661 aagaacataa cttttcgtta tcttctttcc tctttataat tgtttagctt atttagccaa
      721 agcttctttt gcttggtcag ccaatgcagt aaatgctgct gcatcgttaa cagccaagtc
      781 agctaacatt ttacggttga tgtcgatttc agcaactttc aaaccgtgca ttaattttga
      841 gtagctcaag ccattcatac gagctgctgc gttgatacga gcaatccaca atttacggaa
      901 gtcgcgtttc ttttgacgac gatcgcgata tgcgtagttg tatgaattca ttacttgttc
      961 ttttgctgtt ttaaataaag tgtgttttga accgtagtaa ccttttgcta attttagcac
     1021 tcttttacga cgtttacgag ttactgttcc acctttaaca cgtgccatgt ataattcctc
     1081 ctaataaaaa taaatcttca gattctgtta tttgtacttc ttatttcatt tttgctagtt
     1141 gttggcgaat acgtttgaag tcgccactag aaaccatgct tgctttacgc aattgacggc
     1201 gttgtttttt tgttttgccg tggaaacggt gacttgtaaa cgcgcggaag cgtttcaatc
     1261 cgcctttacc agtacgtttg aaacgttttg ctgatccgcg gtgtgttttt tgttttggca
     1321 tgactatatt tcctcctcaa aatctttcgt tatccgacta tcggctgtcc aacagccagt
     1381 tagtcattca aattacttac tgtcgttttt cggtgccagc gtcaagaaca tgctgcgtcc
     1441 gtccattttc gctttttgtt ccactgttgc aatatccgca gtttcttcag ctaagcgatt
     1501 caagactttt tgaccaattt ctttatgggt aatggcacgg cctttgaaac ggatagaagc
     1561 tttcacttta tcgccctttt ccaagaactt acgtgcatta cgaagttttg tattgaagtc
     1621 attcaagtcg atcgtaggac ttaagcgaac ttcttttaca ttgatcactt tttgtttttt
     1681 acgcgcttcg cggtctttct tttgttgttc gaaacggtat ttaccgtagt ccatgattcg
     1741 cgcaactggt ggtttcgcac caggggcaac taacacaaga tccaagtttg cttgttctgc
     1801 aatattcaat gcttctgctt ttgtcttaac tcctaattgc tcaccatctg aaccgatcaa
     1861 tcgtaactct cgtgcacgaa tgccgtcgtt aaccatcata tcctttgcta tggtcattca
     1921 cctccaaata ttatgagaga aagaaaagct taccgaataa acggcaataa aaaaacgagt
     1981 tctcttacag aacccgcgcg tacttcacct aatagaacca gtgaaaacta tctagcccag
     2041 tgacattatc aactaaggcg agaagcgggt gcttctgctt ttatttctca accattatag
     2101 tatagcaaaa actaatcttt ttgtcaatgt ttttcataca gtttgtcttc cttttctgaa
     2161 aatgattaga ttagcaggct ttctagcagt gattcccgct tctttgctat actaaagaaa
     2221 aacgaggtga acccacatga aaaaaatcga acctattcca ccttctcttc ctcttcttac
     2281 aaagggagag ttttatttag aagatgagga gatcttctct ggtcttctga tcaacggagg
     2341 taaccaaagc tatctcgatg ccaaaaatat ggtattaaga gagagtcata tccaaaaact
     2401 tacgatgcaa aaaacaggtt tacgtcattt tgaatgcagt aatgtcattt ttgaaaaatg
     2461 tgatttttcc aatctggagt ggattggtgg cagtttccac caagtcgtgt ttcatcaatg
     2521 taaattaaca ggtacgaatt ttgccgaaag ttatcttcga gactgtactt tcactgattg
     2581 tattgcttcg atggcttcct ttagtagtac aaacttgaaa gctgtctcct tcgatcattg
     2641 tcaattagaa gacagcgaat tttatgaggt cacttggaaa aacctttttt tgtctgacaa
     2701 tcagttaacc ggttcgaact ggtttcgaac ttctctgaag ggattagact ttacgactaa
     2761 tttcttcaca acaatcgctt tatctcaaga ttctttggcg ggactaatcg tcaatcaaga
     2821 gcaagcactt gtgatcgccc aagcccttgg tttgcagatc aaagactaga tcaacgaatc
     2881 cgccgccaca gtgcaataag catccaaata gataagacca aactgatgat caaggcgatt
     2941 tcccatccga aaacggaatg ggtaaagggt aactggagat tcattccgaa gaatcctgtc
     3001 acaatagttg gaacggtcaa gatcagcgac cagaccgtca aaaatttcat cgtgtcattc
     3061 aagttgttat tcagcaaatt attataagtg ccagagagct ggtctaaaat ctgcgaagct
     3121 aagttcgtca tttcaactgc ttgtcgtgct tcgatcagtg catcgtccaa ctcttctttt
     3181 tccttttctg acaacttgcg ataaatagcc agtgccttga tttgttctaa aagtactaca
     3241 ttttgttttg ttcctgtaac taagtaaacc aaaccgatct ctaaatctga aagttgcagc
     3301 agattcttgt tcgttgtttt ttctcttaat ttcagattaa gccgctgacg ttcactattc
     3361 acttcctcga ttaaaggaaa aaaggaatca gaaatcaaaa acaatgtacg aaacaataaa
     3421 ctgtatacac tcatgtctgg catccgttcc aaaagcgatc gaattagtcg aatcacgtac
     3481 tcggtattcg gcgtggtaaa tgtaaacaaa ccgtcttctt ttaaaataaa agcgatagga
     3541 ctagtttcat aatgattatc gactttttgc tggtgcggaa catttgatac aagcagtaac
     3601 gcttcatcaa aagaatcgta ctctacgcgc gcccgttcat ttttatctag agaataagtc
     3661 agcatctcgt cactaatgcc atattcttct tttaagtttt taacagctag cacatcgttt
     3721 tgctgtacat tgatccaaaa attacggtct tgattaaaat agtgtttttc ccgcatactc
     3781 tatcctcctg acatttgttt ccagtataaa gaaaaaagtc gctcaaataa aggtttaccc
     3841 tttatttgag caactgtaaa tcaaatggat actttggttt ggcgaatctc tgctaatttt
     3901 tcttcgatca ttttcaagac ttcttctttt gcttgaggat cttccacaaa attatagcgg
     3961 tctccatcga tttggatttt tggactttct ttgtattctt cataccattg atcataacgt
     4021 ctgttcaatt ctttgtaata atcgtataga cttgggtcaa aggacaattg ttcatacgaa
     4081 cgaccacgtt tttcgatacg ctctaacatc gtatcaaaag atacacgaat atgaactaat
     4141 aaatccggat gttttttatg ggcagcatag ggaagttctt ccatcatgtt ttccagcagt
     4201 tcatcataca cacaaacttc tgtttctgtt gctcttccta gatctgcatt tagatggaaa
     4261 agcagtgaat cttcataaat cgaacggtcc aatacattat tgtcatcttt cattgcttgt
     4321 ttgatgcttg caaaacgttt attcaaaaaa taaatttgca gcaaaaaagc atactgttca
     4381 gggtttgcat aaaataaggg caagacttca ttatcatcaa tcgattcata aaatgcctga
     4441 ctatcaaagt gttcagccat catttctgtt aaacttgatt ttccggcacc aattgtacct
     4501 gctaaaacga tcacgctcat ttacctccag ctcacttcat cactaaatat tgtgtttaac
     4561 tccaaccttt tttaaatata cccccaatat ctagtagtgt caattgtatt ttttctagtc
     4621 aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg ttttttcttt agaaaacgta
     4681 ttcttagttt tacttctgag atggttttag taaaataaag gaggaatgtt ttttgagacc
     4741 cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt ttgggcgctt tattttttgc
     4801 ttaaaaatca cttttacgtg agaaggagtt agattatggc aaactatggc caaggagaat
     4861 caagcggaaa gatcatattg atgggcgagc acgcggttgt ttatggagaa ccagcgattg
     4921 cctttccttt ctatgcaaca aaagtcaccg cattccttga agagctggat gcaatggacg
     4981 atcaactggt ttcttcctac tattcaggaa atttagccga agctcctcat gcattaaaaa
     5041 atatcaaaaa attattcatt cacttaaaaa aacagcatga catccaaaaa aacttgcaac
     5101 tgaccattga aagcacgatt cctgctgaac gtggaatggg atcaagcgct gcagtcgcca
     5161 cagcagtcac tcgtgctttt tatgattact tagcatttcc tttgtctcgt gaaatactat
     5221 tagaaaatgt ccagctttcg gaaaaaatcg cccacggtaa tcctagtgga atcgatgcag
     5281 ccgctactag cagcttgcag ccgatttatt ttacaaaagg gcatcctttt gactactttt
     5341 ctttgaacat cgatgctttt ttgattgtcg ctgatacagg aatcaaagga caaacaagag
     5401 aagccgtcaa agatgtcgct cacctctttg aaacccagcc tcatgaaact ggacaaatga
     5461 tccaaaaatt aggatacttg acgaagcaag caaaacaagc aatcattgag aattcaccag
     5521 aaacgttggc acagacaatg gatgaatcac agtcacttct ggaaaagctg acaatcagca
     5581 atgattttct taatctattg atccaaacag caaaagatac cggggcattg ggtgccaaat
     5641 taactggcgg tggacgcggt ggctgtatga ttgcattagc acaaacaaaa acaaaagccc
     5701 aagaaatcag tcaagcactt gaagatgcgg gtgctgctga aacttggata caaggattag
     5761 gagtacatac ctatgtttaa aggcaaagca cgcgcatata ccaacattgc tctaatcaaa
     5821 tattggggta agaaaaatga agaactcatc cttccaatga acaacagcct ttcgttaaca
     5881 ttggatgcat tttatacaga gacagaagtc atcttttctg atagctatat ggtagatgaa
     5941 ttttatctag atggcacctt acaagacgaa aaagcaacaa aaaaagtcag tcagtttctt
     6001 gacctttttc gtaaagaagc cggcctctca ctaaaagctt cagtaatcag tcagaatttc
     6061 gttcctaccg cagctggatt agcctcctct gccagcgggc tagctgcttt agcaggagct
     6121 tgcaatactg ctcttaagct tggattagac gatctctctc tttcaagatt tgctcgacgc
     6181 gggtctggtt cagcttgccg aagtattttc ggtggtttcg ttgaatggga aaaaggccat
     6241 gacgacttaa gttcttacgc taagccagtc ccttccgatt ctttcgaaga cgatttagca
     6301 atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt ccagcagaaa tgggatgcgt
     6361 cgaacagtcg aaacatccag tttttatcaa ggctggttag attccgttga aggggatcta
     6421 tatcaattga aacaagcaat caaaacaaaa gatttccaac ttctcggaga aacgatggaa
     6481 agaaacggac taaaaatgca tggaacgact ttagctgccc agcctccatt cacttactgg
     6541 tctcctgaca gcttaaaagc gatggatgcg gtgcggcaat tgagaaaaaa aggcattcca
     6601 tgctacttta cgatggatgc tggccctaat gtcaaagttt tagtggaaaa cagccattta
     6661 tctgaggtac aagaaacatt taccaaatta tttagcaaag aacaagtaat tactgcccat
     6721 gctggtccag gaattgcgat tattgaataa agggagattt ttatgattga agtatctgca
     6781 ccaggcaaac tctatattgc cggagaatat gcagttgttg aaacaggaca tccagcagtt
     6841 atcgctgcag tcgatcaatt cgtaacagtc actgtagaat ccgcacgaaa agtcggaagt
     6901 atccaatctg cacaatatag tgggatgcct gtacgttgga caagacgcaa cggagaatta
     6961 gttttagata ttcgggaaaa tccttttcat tatatccttg ctgctattcg tttgactgaa
     7021 aagtatgcac aagaaaaaaa cattctttta tccttttatg atctgaaagt aacaagtgag
     7081 ttagacagtt caaatggccg gaaatatgga ttgggttcta gcggtgccgt gaccgttgca
     7141 acggtcaaag ccttgaatgt tttttacgcg ttgaatttat ctcaattgga gattttcaag
     7201 attgcagcac tagccaattt agcagttcaa gataatggtt cctgcggcga catcgctgct
     7261 agctgttatg gtggctggat cgctttctca accttcgacc atccttggct ccaagaacaa
     7321 gaaactcagc attctatcag tgagttactt gccctggatt ggccaggtct atccattgag
     7381 ccattgattg ctcctgaaga tttacgttta ttgattggtt ggacgggtag ccctgcctct
     7441 acttctgatt tggtcgatca agttcaccgt tcgagagaag acaaaatggt ggcttatcag
     7501 cttttcttaa aaaacagtac agaatgtgtc aacgaaatga tcaaagggtt taaagaaaat
     7561 aatgtaacgt tgatccaaca gatgattcga aaaaaccgac aattactgca tgatttatct
     7621 gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca aattgtgtaa tttagctgaa
     7681 cagtatgaag gagccgcaaa atcttctggt gcaggtggtg gcgattgcgg aatcgtaatt
     7741 gttgaccaga aatctggcat ccttccttta atgagtgcat gggaaaaagc agaaatcact
     7801 ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa accgatgaat cgaaaagacg
     7861 aacatgtttc actagctaaa gcctttcatg ataaacaaaa aaacgaattt gacttcgtac
     7921 ggatcatcca taaccctttg ccgcaaatag cagtttctga tgtcgatcta agtacacagg
     7981 cagttgggtt tacactaagc agcccatttt atatcaatgc gatgactggc ggaagcgaaa
     8041 aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag agaagcagat ttgatgattg
     8101 ccactggttc agtcagcgct gccttgaaag acccttcttt agccgatact tatacgatta
     8161 tgcgacaaga atatcctcac gggaaaatca ttgccaacat cggtgcaggg acttctgtcg
     8221 aaagagccca agaagctatc cgattgtttc acgcagatgc cttacagatt catttgaatg
     8281 cacctcaaga gttggttatg cctgagggag accgtgattt taccaattgg aaagtactta
     8341 ttcaagagac tcaaacagcc attgacgtcc ccctcatcgt aaaagaagtc ggttttggca
     8401 tgacaagaga aacgctcaat gatctagctg ctttaggagt tcatacagtt gatatcagcg
     8461 gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg tcgctccaag cgggaactga
     8521 gttatttagc tgactggggg caatcgacag tctcttcatt gcttgaagca aatgaagcag
     8581 acacttctat ggaaatccta gcctccggag gtatccgcaa tgcctatgac atatttaaag
     8641 ccctttgtct cggcgcaaat gcagtgggta cttcaggaac cgtactgacg catctgatga
     8701 atcacggcgt agaagaaact attatactga tgaagcaatg gcaagaagag cttcgtctgc
     8761 tttatactat ggtcggcgca acgaataccg cggccctaca ccaacaatca ctgatctttt
     8821 ccggaccagt gaaggattgg tgcgaagcaa gagggatcga cttagtgaag tatggaagac
     8881 gcacagaaaa gagcgtggga caaaagtaaa gaacactttt atcccacgct ctaaacacgt
     8941 ataaacggcg gaagcaaaag caactccttc ggaaataagc tgaaattcac aaaaatttga
     9001 aaagcaattt tcgtgaattc cttcttattt cttggagtta aacgcttctg tcccagcctc
     9061 ttttttcgaa taaatgatgt ttaatcagct gtccttcggt attcgttcaa gccgctgatt
     9121 tcacaacctc tccactaaac ggtgtactaa agctctttta aggttgtttt ccgatatagg
     9181 caagtatgcc tccgtccaca tacaagacat ggccgttaac gaaatcagat gcgctagaag
     9241 ccaagaagac agatggtcca gccaaatcga ttggctcgcc ccagcgtgct gctggcgtac
     9301 gtccaacgat aaattgatcg aatggatgac gttcaccgtt ttcttgtgtt tcgcgtaatg
     9361 gagcagtttg cggtgtagca atataacctg gtccaattcc attacattgg atattgtatt
     9421 gaccgtattc agaagcgatg tttttcgtca gcatttttaa tccgcctttt gccgcagcat
     9481 aagcactgac cgtttcgcgt cctaattcac tcatcatgga gcagatattg atgattttac
     9541 cgccgccttt ttcgatcata tcccgaataa ctgcttttga aacgatgaat ggggcattta
     9601 aatccacgtc gattacttgg cggaagtctg ccgcatccat ttcgatcatc ggtgtccgtt
     9661 tgatgatccc tgcattgttg actagaatat caactgaacc tacatcttct ttgatctgtt
     9721 gaaccatcgc ttgaaccgct tcttcatcgg tcacatcgca gacataacct tttgcatcga
     9781 tgcctgcttc tttataatgc gctatacctt catcaactga ttcttgattc aagttattaa
     9841 atacgatttt tgctcctgct gtagctaaag agcgtgcaat ctcaaatcca ataccataga
     9901 cagcaccggt gactaatgct acttttccat ctaaacgaaa catatccata ctaaattcca
     9961 tcattttctc tcccttatgc ttattttaat tgatccattt gcaccatatc catgtctgta
    10021 taggtgatat tttctccgca cattgcccag atgaatgagt aattgcttgt acctacacct
    10081 gaatggatcg accagcttgg cgaaataatg gcttgttcat tgtctacgac taaatgtttt
    10141 gtttcatctg gtttgcccat catatggaac acacgtgtat cttcattggc ataatcaaaa
    10201 tagacataag cttccatacg acgttcatgt gtatgacaag gcatcgtatt ccaagcactt
    10261 cctggttcta aaatcgtata acccatttat aaatgacagc tatcgctgaa gcttctactg
    10321 ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa
    10381 tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt
    10441 tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgcccac aaa
//
LOCUS       DAFEHS010000084        10243 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFEHS010000084
VERSION     DAFEHS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.087"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            229..2394
                     /gene="acm"
                     /locus_tag="LP327_002352"
     CDS             229..2394
                     /gene="acm"
                     /locus_tag="LP327_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBL1726442.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2666..3151)
                     /locus_tag="LP327_002353"
     CDS             complement(2666..3151)
                     /locus_tag="LP327_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBL1726443.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3241..3586
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3631..4272
                     /locus_tag="LP327_002354"
     CDS             3631..4272
                     /locus_tag="LP327_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL1726444.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4291..4926
                     /locus_tag="LP327_002355"
     CDS             4291..4926
                     /locus_tag="LP327_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL1726445.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4944..5759
                     /locus_tag="LP327_002356"
     CDS             4944..5759
                     /locus_tag="LP327_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL1726446.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6078..7514
                     /locus_tag="LP327_002357"
     CDS             6078..7514
                     /locus_tag="LP327_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL1726447.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7703..10150
                     /locus_tag="LP327_002358"
     CDS             7703..10150
                     /locus_tag="LP327_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL1726448.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         3580 a         1674 c         2203 g         2786 t
ORIGIN      
        1 aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat aatttacatt aggaaaaacc
       61 ttgtaaaaac agttttttgg catatttatt ttttataaat atttgtaaaa taactatcaa
      121 ttttttagtt aaaagattgg aaatgagtgg gggaggaatt gaagaaagga tttttgagag
      181 atgatatagt aggttttatt tttagttaaa ataggggaga tagaaaaaat gaaaaagtgt
      241 agaaatatta ttttatcaat gttatttatt attactaata taacatcact gattcctgtt
      301 catgtctatg cggatgcagg cagagatatc agcagtaatg tcacttcgtt gaccgtggac
      361 ccaacgaata ttacagatgg aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa
      421 caaaatatcc aaccaggtga ttatctttgg atcaattggc caagtgaagg aaatattcgc
      481 ggtgaaggat tccaaaaaga aatcccatta atgattgaaa ataaaaatgt tggaacttta
      541 acggtcagaa aagattctgc gcaggttgtg tttaatgaaa atattaaaaa cctagactca
      601 gtagaaggtt gggggcaatt tgagatacag gccagaaacg taaccgatac cggtgaagaa
      661 aatacgggac cattcaccgt gacgagcggt gataaaacag ctactgtaaa tgtgacaaag
      721 ccagcttctg gttcttcttc tagcgttttt tattataaaa caggagacat gctacctgaa
      781 gatactaagc acattcgatg gtttttgaat attaacaata acggcactta tgttgaacaa
      841 ccagttaaaa tcagtgatga aatccaaagt ggacaaagat tagatccaag tacgtttgaa
      901 attaatcaaa tacacttagg tgaacagaag gtttatagag gagaagaggg aatccaacaa
      961 tttttacaag atttccccag cgctacattt aatttttcag taactgataa ttacattgaa
     1021 ataactatac caaaaaactt tgtcaatctt aggaaaataa tggtgagtta taagacgatc
     1081 atagaaaacc cagaacaaat aaattttgag aatcattcgg aagcttggtt taaagaattt
     1141 aataaacctg ctgttgatgg agaaagtttc aatcatacag ttaaaaatat ttcagcttct
     1201 ggtggagtca acggtacagt tagaggcgaa ttgaagattt tcaaatatat caatgacaca
     1261 gagattggta tcccaaatgt gactttcgaa ttaagacgag cagatgaaca gcctattcaa
     1321 ggacagtcta gtatcctgtt gacttcgaat gaacagggag aagctagtat caaaggcctg
     1381 caagtaggag actatgttgt aaaagaaaaa gaagcaccca attggattga ttttgatcca
     1441 ctaagttcaa atgaactcaa attctccata aacgaaaatg atacagaggg agtcagttta
     1501 cctatttata acaagaaaaa ggttacgaac atcacagcaa ctaaaatttg gaacggtgga
     1561 accactccaa gaccgagtat ttattttaaa ttattcagag caagtacaaa caattgggaa
     1621 ccagttcctg atgcagaaac aaaacgatta gacaatggaa taacttcagt gacttgggaa
     1681 gatatccaac agtatgatga tagtggaaac gaatatacgt ttaaagtgca agaagtagat
     1741 caaaacggaa atgattatgt cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt
     1801 acaaatgaga ataaagaagt catttcagtc tcaggacaaa aaacatggga agacaatgag
     1861 aaccaagacg gcaaacgacc gacgacgatc acagtaaatc tattagccga cggtcaaccg
     1921 atccaacaca aagaagtaag tgaaaaagat gattggcgct atcgtttcac aaatctgcca
     1981 aaatacagag acgggcaaga aatcatctat actgtaacgg aagacaacgt gcccgaatac
     2041 agtacgacga ttgacgggta taacatcaaa aacagttata caccggaaaa aacaaatata
     2101 tctataccag gtagccatat aacaccgtgg aaaaattttc cagagaagac agagagagag
     2161 aaaattagaa atctattctc tcaaaaattg caaatatttg ctcatacagg agaatccagc
     2221 gaacaaagaa gcaattatat tagtaagaga gctgtaccta aacagataac aaataaatca
     2281 aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt ttgctgtaat cattggttta
     2341 ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat ataaaaaagt ttagttagaa
     2401 tataatattt tttgttaaat atgactgaaa tcaaaagaat aaaattccga tttataatcg
     2461 aaaaaagaag catttttata tatgcttctt ttttcgtaga atcaataaat atggatttgg
     2521 ctaactatgg aagaatactc gtattagttt tcagcgttca tactagttgt tacattgttt
     2581 gattggccaa aagaatttct gatatatttt gtgctaaata aagaaaaaga cgcttgaatt
     2641 gggaaataag aaaactactc tatcattaca aattttcata aactggcaga tgttctaagt
     2701 aaacaagtat cttttcaaag tcggaaaaat ccataatcaa cgtagtggta ttgatatttg
     2761 gatgaaagcc tacggtatct tgacgatcaa tagcagcatc gatcacgact tggatttttt
     2821 tctctttgtc gtgcatcaat gctaatggag tcactgtacc tgctggaacg cctaggaggt
     2881 caggaagctg ttcttcagaa acaaaagata agcgcttttc ttttaattgt tcagctagtt
     2941 gttttaaatc ggcgtgcttt tcatcaggca agatgacaag atagatttgt tttccttttc
     3001 ttgatttcaa taatagattt tttacttgcg gaccaggtag ggaaaaaggg acatttttga
     3061 ctgacgtaat cgcaggatga tcgacttttt gaaaagagat acccagtttt tttagtagtt
     3121 cataagcttc ttgttcggtt gcacgactca tcgcgcatca ccctcctttt tttattagtg
     3181 tatcataaaa aagagttgac aaatgaaaaa taatcatgta aattacacgt aaagttaaaa
     3241 caaatgacat cgaaaagaag agtagccgtt aggtgttttt agagagtctc tggttggtgg
     3301 aaagaggcag cagttaaccg tgaaacacat cttggagtcg attttctgaa gcgtatagta
     3361 ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta gatgaccaga taaggctggc
     3421 ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg attaacgtcc tttgatcttc
     3481 ggatcaaagg actttttgtt ttcatctgaa cggcaacgac tgtgtgaggt tttttctgca
     3541 aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc ctcttggcat ttgccaagag
     3601 gattttttta ttttaaagga ggaaaagaag atggagtatt tgattgaagt tatgccgcaa
     3661 ttattagtag gagcactcac aacattgaaa ttatttttat ttaccttgat tggctcatta
     3721 ccattaggga ttgttttagc attaggattg tctttgcatt ttaagccgct acgctacttg
     3781 cttcaaacat atgtatgggt tatgcgggga acaccgctgt tgttgcaact gatttttatt
     3841 ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt tcgaagcagc actcattgcc
     3901 tttatcttaa actatgcagc ttattttgca gagatctttc gtggagggat acaatcgatt
     3961 cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa gtccctttca aacggtgacg
     4021 aaaatcgtca tcccacaagt gacaaagatt gttttacctt ctgtagggaa tgaagttatc
     4081 aacttggtca aagattcttc cttgatctat atattaggtt taggtgacgt aatgcgtgct
     4141 gggaaaatcg cgatggaacg cgacgcgact ttactgcctt tgctaggcgt ggcaatcatc
     4201 tatcttacat tgactggtat tctaacaatc gtaatgaaac aagttgaagg aaaattaaat
     4261 tattaccgct aactgatagg aggaagaaat atgattgaat tgaagaaagt gaacaaagca
     4321 tttggtgaaa aaaaaataat caaagatttg aatctaatca ttccagatgg gaaaacatta
     4381 gctatcgtag gtccttccgg tggcggtaaa accacgcttt tacgtatctt ggctggctta
     4441 gaaagttctg acagcggaga attttattta gacggtgtac cgttcaaccc attcgagctt
     4501 aaaatgaaag aacaagttgt aggagtcgta ttccaagact tccagctatt tccacatctg
     4561 aatatttttg aaaacattac cttagcacca aaaatggtat tgaaacagac aaaagaagaa
     4621 tacaccaaaa aagcagaaga attagcggct tcattaggga ttagtgattt attgaatatc
     4681 tatccttatc agctttctgg cggacaaaaa cagcgggtag cgattgccag agcattggct
     4741 atgaatccac gggtattggc ttatgatgaa ccaacaagtg ctttagatcc tgaattacgc
     4801 cagcaagtcg agcgtttgat cctatcattg aaagaagatg gggtaaccca aattattgtc
     4861 acccatgatt tagtatttgc agaaaatgtc ggtgatcaat tactaaaagt ttccccaatt
     4921 cattaagaaa ggagaagata gagatgaaaa aaaccagttt tctattcgct ttgatcgcag
     4981 gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc agcagataat tgggcgacta
     5041 tcaacgaaga gaaaaaaatc gttataggtt tagacgatac atttgttcct atgggattta
     5101 gagataaaga cgggaagctc acaggatttg atattgattt agccaaagca gtctttgaac
     5161 aatatgggat tacggtagac tttcagccca ttgactggtc aatgaaggaa tttgaactga
     5221 acaatggaac gatcgatttg atatggaacg gctattccaa aacgccggca agagaaaaga
     5281 aagtgcaatt tacaaaacct tatatggaaa acgatcaagt gctgatcaca cctaaagaat
     5341 caggaatcac aaactttgga caaatgaata ataagagatt aggtgcgcaa aatgggtcat
     5401 ctggttatga tgtatttacg aaacaaccag aagtattaaa agagatagtc aaaaaccaag
     5461 atgctgtttt gtatgatagt tttaatgaag cattgatcga tctgaaaagc agacgggtcg
     5521 atggtctgct gatggataaa gtatacgcag actattattt gaaacaaaga aacgaactag
     5581 aagacttcaa cataacaaaa ggttcttatg agagtgagga ttttgcagtt ggtgcacgca
     5641 agtcagatag tacattagtg aaaaaaatca atgaagccct agatcaacta gagaaaacag
     5701 gtgaattcca aaaaatatcc gataaatggt ttggcgaaga tgttacgccg agaaattaac
     5761 aaatagggga ctgtgacaaa agacgtgtca cagtcccttt ttccgaataa acagtgttct
     5821 cctgccaact ccttgtgact cggagctgaa tggcaggctc acaacctctt ggtaaacggt
     5881 gttcaaaagc cagtttttcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt
     5941 tcacaaaatt tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat
     6001 ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat
     6061 aggaaaggaa gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt
     6121 ggggctctgc ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag
     6181 aatcagtctg ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg
     6241 atttggcagt agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag
     6301 gtctgaaagc ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag
     6361 aagtcaatca agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg
     6421 atattgaacc tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat
     6481 ataatggttg ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat
     6541 ttgaggcatt ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca
     6601 cttcattagg ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt
     6661 atgaagttaa ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt
     6721 tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca
     6781 gtaatcctga aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga
     6841 tggatgtcta tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag
     6901 gatgggcacc aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt
     6961 ttcttgggat caattactat caaacggcga cgaatgcatt caatccatta aacggagttg
     7021 gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg
     7081 gaatgtataa aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg
     7141 acccagaagg attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac
     7201 tgattacgga aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg
     7261 acgactatcg aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg
     7321 atggagcaga agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga
     7381 acggttatca aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat
     7441 ctttggaacg atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg
     7501 gagagaacct ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct
     7561 tttcttgata aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag
     7621 aaagcgtcag ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac
     7681 ttgatgaaat gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa
     7741 gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg
     7801 gtttgccttg attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt
     7861 gattgcaaac ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt
     7921 agccgtatta ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg
     7981 acgctggttt ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt
     8041 atttggcaaa ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc
     8101 cagtgatata aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat
     8161 cctttatcat tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct
     8221 attactggca ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg
     8281 tatccaatca attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc
     8341 tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag
     8401 gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc
     8461 ggtgagccag caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc
     8521 ggagcagcta agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct
     8581 agagaaacca gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga
     8641 gttgccagag gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca
     8701 agactatgaa ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga
     8761 tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg
     8821 tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca
     8881 gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc
     8941 tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat
     9001 tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc
     9061 aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca
     9121 aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa
     9181 gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt
     9241 aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca
     9301 tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt
     9361 acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg
     9421 ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc
     9481 attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt
     9541 tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct
     9601 agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc
     9661 cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg
     9721 ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc
     9781 gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt
     9841 tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac
     9901 gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt
     9961 tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg
    10021 agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag
    10081 caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa
    10141 tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg
    10201 catgatgata ccaaaagaaa aaatggtttt tttccatctt ttc
//
LOCUS       DAFEHS010000085        10226 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFEHS010000085
VERSION     DAFEHS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.088"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(560..1600)
                     /locus_tag="LP327_002359"
     CDS             complement(560..1600)
                     /locus_tag="LP327_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL1726449.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="LP327_002360"
     CDS             complement(1949..2275)
                     /locus_tag="LP327_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726450.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3065)
                     /locus_tag="LP327_002361"
     CDS             complement(2262..3065)
                     /locus_tag="LP327_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL1726451.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="LP327_002362"
     CDS             complement(3418..3708)
                     /locus_tag="LP327_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL1726452.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="LP327_002363"
     CDS             complement(3844..4023)
                     /locus_tag="LP327_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726453.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="LP327_002364"
     CDS             complement(4020..4370)
                     /locus_tag="LP327_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726454.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="LP327_002365"
     CDS             complement(4363..5688)
                     /locus_tag="LP327_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL1726455.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="LP327_002366"
     CDS             6138..6752
                     /locus_tag="LP327_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL1726456.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..7921)
                     /locus_tag="LP327_002367"
     CDS             complement(6917..7921)
                     /locus_tag="LP327_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726457.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(8380..8637)
                     /locus_tag="LP327_002368"
     CDS             complement(8380..8637)
                     /locus_tag="LP327_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL1726458.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8630..8899)
                     /locus_tag="LP327_002369"
     CDS             complement(8630..8899)
                     /locus_tag="LP327_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL1726459.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(9030..9209)
                     /locus_tag="LP327_002370"
     CDS             complement(9030..9209)
                     /locus_tag="LP327_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726460.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(9590..>10226)
                     /locus_tag="LP327_002371"
     CDS             complement(9590..>10226)
                     /locus_tag="LP327_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726461.1"
                     /translation="EVIRRIQKDFSCMEEMKYFIQRVEEQLYLESETSQTKVTVSHNR
                     LDVYKRRPETVKKGTELLRLETEKYIMNVYLERSELVENFVSYTICRKHYYSESEYYS
                     IFESMELMEEEMEELNSKDATIDDYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLRE
                     YVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3122 a         1870 c         1464 g         3770 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tattctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattaga gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctatca ttaattttat aaaaaaaatt aaatgttttt agcactgcca
     7201 tataacattc aaaacattct tctatagttg gtttacttcc ttgaggagaa tgaaccaatt
     7261 tattacgttt ttctatgaaa tttttaattt cctttcttat ttctttcgga aactcaatat
     7321 taaagtaccc tttaaacatg ctccctaata atttgtctac agggggagat tgtaaattct
     7381 caaatattag agttaaatca ggtttctctt gttgataaaa ttcctttacc cctatttcac
     7441 aggctgtaat tatcgaaata aacctatctc tagtatttga tatattttta cttcttttaa
     7501 ggagtataag agaaaagggg atactttcgt gtccatgcat gtaattaact aattgttgag
     7561 cttcagaagt tatatctcta attatatagt taacggaatt aggaagtgtc tttaactcta
     7621 atgaaatttc tatccaatta ctatcttcta atctgtatat gctcgaagta taatttgatt
     7681 cgtcagcaag catcgctaca tcccaaacac tgatcataaa atcttttaaa cgttcaatca
     7741 tttcagcaat attgctaaaa tcagctaatt tttctttaga ataaaaaaaa acatttatat
     7801 cagaatcaat aaattctatt ttttttatta ctatatcgtc atcattatct tgaccattta
     7861 tgtttattgt tccctgagtg gggagtactt ctttcaaatt caagttaaat ttatattcca
     7921 ttacttctcc tccaatatat aaaaattact taactgtatc caaatttaat atgtaaataa
     7981 atcattaatt tttagctaaa ttctatgaga agtttatttt tgttgttttt gctaattaac
     8041 taaaactagt acaaaaaatt taaaatagat tattaaacct attgcttatt ctattcagtc
     8101 atatatataa gcgtcatcct ttagtaacta acatcccaca ctacaatagt attataacat
     8161 atctattcaa cttcaaattg ttgaaattta agccatttat atttactttt cctcaacttt
     8221 agaaatgatt ttactaacct tatatccgta atacgcgtaa caaacacatt cattaataac
     8281 aatcaacact aagctatatt agctttttaa atgactaggg taaataaaat aaggatcaaa
     8341 aacttttaag tttctgaccc tttccttact tccgattggt taatagtgat cttttgcaga
     8401 ataaataaat atcgtttcat tttcaactct atatatcagt ctatgttcat ctgtaattct
     8461 tctggaccat tttccagata aatcatgctt taatggctca ggttttccta atccagcaaa
     8521 gggggaacga tcgatatctt ttattaactt gttaatcttt tttatattgc ttttgtttcc
     8581 ttgctcatgc caataaagat aatcatccca agcatcatca gaccaagcct taatcatcag
     8641 attcaacctc gattaagtca tgtgttttaa atgcaccttt ggagaattgt tcatctcctc
     8701 gacgaatttt ttccatgacg taattattag aaagtgttct caacgtttct tgcatagaat
     8761 cataatctct ttttgataat acaacaactg tatcttctac atctttactt gttacaataa
     8821 gtgtttcagc atcctcatta acttgtttca tataactacg taaattttgg cggaaatttg
     8881 aataagctac tgcttccatt cctttcaccc cttatttatt tctataaaac aattgtacat
     8941 tatattgtac aattaagcaa atgattaata ttctaacttc ttataattaa aagtgctatc
     9001 acttttagtt tttaggataa aaagtgacgt cactttttga gtgctggtac ctcaaatcct
     9061 gctttttctt gggcaatcaa taaatctaac gcaatacaca taatcctgga ttcacttttg
     9121 ccgtacttct gctttagttg ctgcaattct ttttgtctct tttcatccaa cgtaacgggt
     9181 aaccgtttat ttcctattgc aattggcatt agtactctct cctttcatcg aaataaccct
     9241 atgaaaaata tggctgcaat catcttgaat agtagactca caagggctaa tacaaagcgc
     9301 caaaagatca aacaaaatac cagtattgcc aaaaatacta cgaagtttaa atgcacagct
     9361 gcagcatggc gcataagtat ttcgccaaca aataggacta acaatagagg aatgatttta
     9421 ttcatatgga tctccttctt tctcttaggt tatcgggtgt tgcccgctta tttcgcacca
     9481 cctaagcggc gggggcggtt ggagcgattg gtgcgaaata agaaccattc ccttagttac
     9541 taggtttttt gtccttattt cacacattct cacttttcgg tataaatgat tattgccttt
     9601 ttttcctttc aaatcgttga tatggcattc gtttacggtc aagttcattt tggatttctt
     9661 ctgtatcgtc gtaagtatag gtgtctaaaa aatcagttaa agtcgtgtac cagtcgtcgc
     9721 ctgggttatc tacttcttct acatattccc tcaaaaatgc ttcgggtaca gcgagttcat
     9781 aatccattcc cattaacacg atagattccg ttccacacgt caaaaatcta gtaaatacaa
     9841 ggtaataatc gtctatcgtc gcatctttgc tgtttagctc ttccatttct tcttccatta
     9901 attccatgct ctcaaaaatt gaataatatt ctgattctga gtaatagtgt ttcctgcaaa
     9961 tcgtgtagct gacaaagttt tcgaccagct cactcctttc taggtaaaca ttcatgatgt
    10021 atttttctgt ttctagtcgt agaagctccg ttcctttttt taccgtttca ggacgacgtt
    10081 tatacacgtc taaacgatta tggctgactg tgaccttcgt ttgactcgtt tcactttcca
    10141 aatacaattg ttcttcgact ctttgaataa agtacttcat ttcttccata caactaaagt
    10201 ctttttgaat ccgtctgatt acttca
//
LOCUS       DAFEHS010000086         9874 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFEHS010000086
VERSION     DAFEHS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.090"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(22..267)
                     /locus_tag="LP327_002372"
     CDS             complement(22..267)
                     /locus_tag="LP327_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBL1726462.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(382..960)
                     /locus_tag="LP327_002373"
     CDS             complement(382..960)
                     /locus_tag="LP327_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL1726463.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1037..1567)
                     /locus_tag="LP327_002374"
     CDS             complement(1037..1567)
                     /locus_tag="LP327_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBL1726464.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1571..2431)
                     /gene="rlmB"
                     /locus_tag="LP327_002375"
     CDS             complement(1571..2431)
                     /gene="rlmB"
                     /locus_tag="LP327_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBL1726465.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2424..2819)
                     /locus_tag="LP327_002376"
     CDS             complement(2424..2819)
                     /locus_tag="LP327_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBL1726466.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2816..4228)
                     /locus_tag="LP327_002377"
     CDS             complement(2816..4228)
                     /locus_tag="LP327_002377"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBL1726467.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4225..4761)
                     /gene="cysE"
                     /locus_tag="LP327_002378"
     CDS             complement(4225..4761)
                     /gene="cysE"
                     /locus_tag="LP327_002378"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBL1726468.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4847..5086)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5083..6543)
                     /locus_tag="LP327_002379"
     CDS             complement(5083..6543)
                     /locus_tag="LP327_002379"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBL1726469.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6713..7870)
                     /locus_tag="LP327_002380"
     CDS             complement(6713..7870)
                     /locus_tag="LP327_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBL1726470.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7904..9274)
                     /gene="radA"
                     /locus_tag="LP327_002381"
     CDS             complement(7904..9274)
                     /gene="radA"
                     /locus_tag="LP327_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBL1726471.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9337..9864)
                     /locus_tag="LP327_002382"
     CDS             complement(9337..9864)
                     /locus_tag="LP327_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBL1726472.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2509 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaaagaaat gcaataaaaa taaataagaa
      361 gctcattaag atttttctaa attattcctc attataaaat ttcaagtgtt tttttatttt
      421 gagttttact ctcccgtaag cgctcattac ttgcttgtga tccattgcta aacattcagc
      481 catgtctaca aaatcaacgc cttgcatata ataatgaaaa attttagctt ctatttttga
      541 aaactggatt tcgcaatttt cgattgcttc ttttaataaa agatgctgaa tcgctaaagg
      601 ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct gtctctattt tttggtcgat
      661 tgagacttca tctttttgat cacgacgctt cagcgcgttc tgcttcctca acagagaata
      721 gatttgattg cttataatgc ttttataata acttccaaag ctaacacctt tgcttttttc
      781 gtaagttctc aatgcctgat ataaagcgat ccttgcttct tgttcccaat cctcagaaac
      841 ataatttctt aaataatagg tgtttttcaa acgtagtaaa agtggaaaat atttttgata
      901 cagggcagca aatgcttctt cgtcaccatt acgtgcagcg cgcaataact cctcagacat
      961 aaaataattc ctccataaat tcgtttatgg aggaatctta tcatcaatga ttcatcgaac
     1021 tgattagaac gatatgttat ttattttttc tcattaaatc gtccaatttt tgggacaatt
     1081 tttttaactg ctcttcattc catggagaat tacgtcggaa atcaataaaa tgcatatctt
     1141 tattttcttg ggctattgtt ttttccgttt tttttatcgt cttatataat tcataagcag
     1201 aagttcgtaa tgctccttgg gaaaagatca tccactgttc tgctaaatcg cttgtcgcca
     1261 ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat tctctcgata taactgtcag
     1321 ccgtttcgtc ttctttcgta aaaacaacat ccaactggta tttcttataa ttttgttgga
     1381 tgcccggaac tagttgggca tcgaagacaa cgatgacttc cattccttca tatttcgcat
     1441 aatttgataa acgatgtaaa agagcttctc ttgcatccgc cattttgtcc tgctttttaa
     1501 gctggacaag ttccggccac gaaccaatca tgttgtagcc gtctacaatc aacagctgtt
     1561 ttttcatggt ctactccccc tgacgttggg aaaaagcctg atacataagc aaaccagcag
     1621 caacactggc atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca
     1681 tttctttttt caatcctgga ctcattcctt tgccttcatt accaatgact aaagcaattg
     1741 atcctttggc attccatttt tggaagtttg tgccggtcat atctgtgcca aatacccaaa
     1801 atcctgtttc tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga
     1861 catgttcgac tgccccagtg gaagtctttg ttacgatagg tgtgattcca actgctcggt
     1921 gttttggtat gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa
     1981 aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt
     2041 ttgttcgttc aatcagttgg tcaagggata aatattcata aggtgtaatc gcaagaacga
     2101 ctccttgatg cacgccgtga tcgctcaaag tttctaattt ttgtttagga acccatttga
     2161 ctggcacagc ttgttcacgt gcgatttctt tcagttcttc tactttttct cctcgtgaat
     2221 cttcttgaag aaagagttta ttccctcgcc cttgttgtag cgcttctact gcggcatggt
     2281 gcccaaagac gaaattatct tctaatgctt cttcattttg atcagaaact gactgatttc
     2341 gtccagtttg ctttttattt gaatatctat tttgtttttt gtcttcaaat ccacgtttcg
     2401 attgatgttt gtttgttctt tctttaccca tttttttcac caactttctg aatacaccag
     2461 cggatcaatt cttccatccg ctcggtttgt tttgtcaaat gcagatagcc catgagcgct
     2521 tcgaaccctg ttgcgacccg atacgtcgta acatcagcat tttttgcgct cgtatggctt
     2581 ttagcatttc ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag atgttcttcc
     2641 agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga
     2701 tgcagcttat tgggttttgt ttctccttga tcaatcaggt ggtcacgaac atagatttcg
     2761 taaatcgcat ctccaacata agccaaggct aaaccgttca attgtgtgta atctctcatg
     2821 cttctcttct ccatcgggtt ccttgcggtg tatcttctaa taagatccct ttttctttca
     2881 acaaatcacg aatttcgtcg cttcggacaa aatcacgatt gcttcttgct tgatttcgtt
     2941 cttcgatcaa ttgttcaatc tcttcatcta ataactcttc tgctgtaaat aaaataccga
     3001 agatcgaaag ccattgcgag aataaagctt ctccttgtgc caagacagcg gcagaaactt
     3061 tcggctggtc tagataagtg ttcagccact tagcaagctc atagaccact gtgatcccat
     3121 ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa ttttctaaca
     3181 aagcgatttt atccgcatca tctgccaaat tctctgaggc agtttcttgg cggaatttca
     3241 aattgtcaaa cgtattcttc agtcgttgta aattggtggc tgcttctttc atcgtcgttt
     3301 cactgtagcg gatcggacga cggtattgtg tggtcgccat gaagaaacgc aagacttgtg
     3361 gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt ccctagagat ttactcattt
     3421 tttcatcgtc ttcgccaatc gttacatagc cattgtgcat ccagtaatta gcgaattttt
     3481 tccctgtttt cgcttcactt tgtgcgattt cattttcatg atgaggaaat tctaaatctt
     3541 gcccgccacc atggatatcg atcgtttctc ctaaatgttt tgttgccata acggaacatt
     3601 caatatgcca accgggacgt cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt
     3661 ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg
     3721 tacgctgact tgctccgact tccagttcat cgatggattg gtcacttaat ttcccataat
     3781 tttcaaattt acgggtacgg taatacacat cacctgaaga ttcataagca tagccttttt
     3841 caaccaacac ttgaataaat gctaaaatat ctggcatatg atccatgact cttggatgca
     3901 gtgtagcagg ctcgacattc aacgctttcg tatcttcttc aaatgctcgg atgaaacgat
     3961 cagcaacttc tggtgctgta atacctaatt cgtttgctgt acggatgatt ttatcgtcta
     4021 catccgtgaa attcgatacg aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa
     4081 ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg gatataatta tacacagtcg
     4141 gtccgcagac atacataccg acatgtccct tttgtatagg gatgaattct tctttttttc
     4201 tggtcaatgt attgtagatt tttatcattc tgatctcctc actttttctc ctttgattcg
     4261 aacgactttt gctggtactc ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac
     4321 ggcacctgca ccgattttgg cgttttttcc gatcgttacc ggtcctaata tctgcgcatt
     4381 cgcagagagc atggctcctt gtttaacagt tgggtgtctt ttacccgttt cttttcctgt
     4441 gccgcctaat gttacgccat ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc
     4501 aatgacgatt cccattccat ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg
     4561 gatctcaaca ccagttagga aacgccagaa ttgagcattg atcttcgcca ataaaaagag
     4621 ccggtgctga tataaaaaat gagagaaacg atgccaaaac aaggcatgaa aaccagggta
     4681 tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg tttctctttg ctgcatcaat
     4741 cgctcgggta aaccaactca tgactttcca ctcctttcgt aagaatttta tgtaggaaat
     4801 catcttctta taaagaaaag aagcgccttt taaataagtt tccttatcaa aagacgcttt
     4861 ccgcgtggtt ccacttttct tcataatcaa tgattatctc tgacagctaa cgcctgttat
     4921 acgttcttgg ctactttgtt tcctataaaa cagtttttcc tgttttctgt gtttcgccaa
     4981 gaccactccc agaggcattt cggcaaccat tcattgatac cttgcaccac ccggtatctc
     5041 tcttgtaatg atcagctgct tacttttttc tgttcatcgt gtttagttca gtacttgatt
     5101 taaatgtgct aatgattttt cacgaccaag caattcgatc gtttcaggca attctggacc
     5161 atgcatttgt cctgatacag ctacacggat tggcatgaaa agattttttc cttttacgcc
     5221 agtttctttt tgaacagctt tgatggcagc tttgacagtt ggagcatcta ctacttccat
     5281 ttgttctagc tgtgctttga atgcgtggag aacagtagga actgtttcac cagcaagtac
     5341 ttcttttgct gcttcattca atacaggatg ttcattgaaa aatagcgtag acagatcaac
     5401 gatttctgca gcatagctca tttgaggttg atacaggcta acgatcttct tcacccattc
     5461 gattgtcttt tcatctgggt tttctgctac acgtccatca gcgattagat aaggcagaca
     5521 catgtcagta aatgcatcta gatccattgc tttgatgtaa tggttgttta cccattgcag
     5581 ttttttcgca tcgaaagctg cgggtgattt acttaggcgt tttggatcga acattttgat
     5641 caattcttgg tgttcgaaga tttcgtcttc gcccacaggt gaccagccca ataatgcaat
     5701 aaagttgaac attgcatctg gtagatagcc taattcgcga tattgttcga taaattgcaa
     5761 gattgattcg tcacgtttgc ttagtttttt acctgtttca ctgttgatga tcaatgtcat
     5821 atgtccgaag gttggtggtg tccagccaaa tgcttcatag atcatcagtt gttttggtgt
     5881 attggcaata tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac
     5941 cgcaacagca aaattataag ttggcatacc atcgcgtttt tgtatgacaa agtcgccacc
     6001 gatattatcg gattcgaacg aaatagcgcc tttcaccata tctgtaaatg cgtattctgt
     6061 attgcgaggc acacggaaac gtacaacaga ttctagacct tgtgcttctt tttctgcttg
     6121 ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg
     6181 ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt
     6241 gctagcaagc agctgatcaa tcagcggttg atagatttct ttacgttcag attgacggta
     6301 agggccgtat tctccaggat tttcaggaga ttcatcccaa ttcattccca accaagcaag
     6361 gttttccaac tggctttttt cgccatcttc gatattacgt ttttggtcag tatcctcaat
     6421 acgaataata aagtctccat cattgtggcg ggcaaacaaa taattgaata atgcagttct
     6481 tgcatttcca atatgcaggt gcccagttgg acttggtgca tagcgtacgc gtactttcgt
     6541 cattttcttc ctcttctttc aaactagact ttgactaaca gcaaaaattc cgtcagttac
     6601 ggcttctcaa atatcattta tttaaagcca ctcagcacaa attttacctt ttctaaggta
     6661 aatagtaaag ccaactggtt gaatttcctt accagttggc tgaatttctt ttttattttg
     6721 attgtttagt ctttttctct gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag
     6781 gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc acttgttaca acgacttcga
     6841 tacgttcatt catgtaatgc tgtccatctt ctacgacaac catcgtgccg tcatcaaggt
     6901 aggctactcc ttgttggcgt tctgtcccgg cttttactac tagaacattc atcgtttcac
     6961 cagggatgac gactggtttt acggcatttg ccaacgcatt gatattaaga acaggtacat
     7021 tttggaattc agagacttta ttcaagttat agtcattggt tacaatgaca ccatctaaaa
     7081 gcttagccaa tttgatcaat ttgctgtcta cttcactgat atcttcaaag tctccatcgt
     7141 acatttcgac agatataccc tcttcttttt gcaaggcatt taggatatct aatccgcgac
     7201 gaccacgcac acgtttcaaa ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa
     7261 caaaattagg gatcatcaag gttccttcta agaatccagt ctttgtaata tcataaatcc
     7321 gaccgtcaat gatcacgctt gtatccaaga ttttatattt atggaaattt tctcctgatt
     7381 ttcgttctaa cagttgactc tcaacttgac tagctgtttc ttgctcgatt tttttgcttc
     7441 gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt tcccattctc aatcccagat
     7501 agcctaagat gatcatcagt aaaatcggca atacactatt gacgaatgga atttccatat
     7561 tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc gatgatcgca ccgatcgcac
     7621 cgaagagaag ataagtcaag ctgacctcac ttagtttttg ttctacttgt ttgataatag
     7681 ctgagatatg tttggccaac agcaaagata ataaaaagaa aataagtgcg cctaacaggc
     7741 tgttggtaaa tgtattattc agccatccgt ttgactgttg accgattgcc tgccaagctg
     7801 agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat catcagcaaa gtaatgacac
     7861 gtttttgcat cgtttttcct ccgtttcgac aatcatttat tagttagcgg aacacacgct
     7921 tcaacgtttc agccaaagtt gctactccaa ctatctcgat tccttttggc gcttcccagt
     7981 tacctaagtt gtttttaggt aggtatactt ttgtgaagcc gagtttttgg acttctttga
     8041 ctcgttgttc aatatgactg acacgtctga tttctccagt cagtccaatt tcaccgatga
     8101 aacattccgt tggctgtgtt cctttttctt tatagcttga agcaatactg acagcgatag
     8161 ctaaatcaat cgctggttca tttaatttta ccccacctgc agcttttaag taagcatctt
     8221 gattctgaag aagcaaccca gctctttttt ccagcactgc catgatcaga gatacccgat
     8281 tgaaatcaag acctgttgtt gttcgtttgg catttccgaa catagtaggt gtgactaatg
     8341 cttggatctc taccaaaatt ggacgtgtcc cttccattgc cacaacgata gcagagccag
     8401 ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg attagcgact tcttccaatc
     8461 catgctcatg catttcaaaa atcccaatct cattcgttga gccaaatcgg ttttttactg
     8521 ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa atataacacc gtatccacca
     8581 tatgttctaa cattcttggg ccggcgatcg agccttcttt ggtaacatga ccgacgataa
     8641 aaatggctat cccatttgtt ttagcgattt tgagtagttc tgcagtggtt tctcttactt
     8701 ggctgacact tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa
     8761 tgataacata atcaggggag atatgttcga tggcacgact gatttcgttc atatctgttt
     8821 cagcgtatag ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt aattttatct
     8881 gctctgcgct ttcttcgccg gatacatata agacttttcc gcctatggct gctaattgct
     8941 gagaaacttg taacagcaaa gtggatttcc cgatacctgg atcgccacca atcaagacca
     9001 tagaccctgg caccacacct ccgcctaaca cacggttcag ttcttctaat tttgttttga
     9061 tccgaggttc ttttttaggg acgacgtctg cgatttttgt tggttttgct ttttctcctg
     9121 tcaaacttgt ccgagttctg cggtccgttg tatcttgttc gatttcttcg accatcgtat
     9181 tccattttcc gcaatttggg cagcgtccca agaacttagg cgacacatac ccgcaatttt
     9241 ggcaaacaaa ttgtactttt gatttttttg ccattgtacc gcctcctttt atcctaatca
     9301 tgagacatta aatcaagctt aagtatcttt aacgttttat ttactggaag aaccaaatcc
     9361 gccttgacgt tgagcagtcg tttgatcttg atctgctttt aaaaacggca aaaagatccc
     9421 ttggccgata cgctcgcctt ttttgatcac tacatccttg aaaccaaagt tggtgaattg
     9481 gaacatgata tggccttcat ttgcctcatt attgtaataa tcactgtcaa tcacacctac
     9541 tccgtttgtc aacactaaaa accgcttcaa cggattgctt gaacggttag caagctgcaa
     9601 aaattcatct tctcccatgt aagctttgat tccagtaggc acgagggttg gtttcaattg
     9661 tttttcattt tctgatacat cttttttcaa tccatctgcg actgtttcca accccttttt
     9721 ccagcctgct ttccagatac ttggaatcac gacctcttct gctgcttcaa aatcatagcc
     9781 agcagcatga tgtgtagcgc gctgcggtag attgatattt tgatctttat atttagaaat
     9841 aacttcaaat cctctttttc tcaccggttt tcct
//
LOCUS       DAFEHS010000087         9722 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFEHS010000087
VERSION     DAFEHS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.091"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            176..1567
                     /locus_tag="LP327_002383"
     CDS             176..1567
                     /locus_tag="LP327_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726473.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1640..2287)
                     /locus_tag="LP327_002384"
     CDS             complement(1640..2287)
                     /locus_tag="LP327_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL1726474.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2295..2981)
                     /locus_tag="LP327_002385"
     CDS             complement(2295..2981)
                     /locus_tag="LP327_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726475.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2987..4060)
                     /locus_tag="LP327_002386"
     CDS             complement(2987..4060)
                     /locus_tag="LP327_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1726476.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4282..4956
                     /locus_tag="LP327_002387"
     CDS             4282..4956
                     /locus_tag="LP327_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1726477.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKL
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4961..6301
                     /locus_tag="LP327_002388"
     CDS             4961..6301
                     /locus_tag="LP327_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL1726478.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6450..7436)
                     /locus_tag="LP327_002389"
     CDS             complement(6450..7436)
                     /locus_tag="LP327_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBL1726479.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            8160..9191
                     /locus_tag="LP327_002390"
     CDS             8160..9191
                     /locus_tag="LP327_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL1726480.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            9300..>9722
                     /locus_tag="LP327_002391"
     CDS             9300..>9722
                     /locus_tag="LP327_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL1726481.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT         3124 a         1754 c         1672 g         3172 t
ORIGIN      
        1 ttattttaat atgataatta ttatacgaag ggcgctaaag ggaatcacac caattttcat
       61 accaattggt ttcatttatg aagttgattc cttcgattaa tgagcgtttc attgaaagaa
      121 aagggttgag ggagaaaaac atatgatcag gttgttggaa cgaaactata gaaagatgga
      181 aaaaatcatt cgaattctgt ccatttctga caagtggatg actaaaaaag aacttgccaa
      241 acgaattggt agttcagaat ctacatttgt aaggtatatc gaagagatca aaatccgctg
      301 gggaacggat gtaacgattc aatcttcaca aaaattgggg tatcgtcttg aacatttgaa
      361 tgtaccagct tatttaaatg ttctgatgga aatgtcgcaa agttctacaa acagtcgttt
      421 attaaatgag attattgtca atccgggtaa aacgattgag tattattgtg aaaaaatttc
      481 catcagccgg tcttctttta cacgtaagtt gaaacaatgc aatcaaattt tagaaagcta
      541 tttattaaaa gtgatcgttg accaaggttt tcagttaact agccttaaaa gtgagctcca
      601 gttgcgtatc tttgttacct ttttcttttt aagttactac ggaagacgca cgttgccatt
      661 tccaatcgaa aaaaagaaga tcaaagaaat tatggtcaag aataattgtc agatgaattt
      721 gtttcaggaa gaaagcccat atgagcaatc tttctttatt atgtattttg ttgtgcatct
      781 catgagagaa tcccaaggaa ctccatttga atggaaagct ggagactctt atgtacagtt
      841 gataaatatc gataaaaaag acttcttaat cttgaaaaat ctcttaggaa aattgaagta
      901 cagcagctat ataaaagtag tgaactattt tgtcaatacg attttttcag aattttcgga
      961 gaaagaaaaa aatgaaatca gtgagcagat acgaaaaaat atgtcctact gttcgttttc
     1021 atccgctgat tgcaaagaaa aaaacgaaga atatgttgtt caattattaa cgcaaattta
     1081 ttttttctcc cgtattttcc catacgatac gactcatttg acgatccgtg caacttactt
     1141 tgcacagcag atgcaacacc gccagatacg tctatatgct ggatttcgtg cagaacttca
     1201 attttattcc actctactga atcagaatct gatttattat ttatctagct atatcttttg
     1261 gatgcttatc ggtaatcctg aaattcacca ttatacttct gataaaaaaa tactgattat
     1321 cagcgactta agtcttcgac atagtcaata tattgaagaa tatatcagtg atattcttgc
     1381 tgtgcataaa attcactctg agacaactgc tattactgaa gatcagctgt cgaaatacaa
     1441 tcttctcgag tatgatctga tcgttacgaa tcaacccatt ttgaatgccc atgttcctca
     1501 tatattgatt gatgatagtg tgtcattcgc aaatgaagaa gaactcatcc gcttattcga
     1561 gttatgaata atcaaaaaac agctatgtat aaaccaccct gataaaaatt tcaggacaaa
     1621 tacacatagc tgtttctttt tattgtattc cttcagcaat ccaacgagat ttatagcgaa
     1681 agtcttttaa caactcttct tttgtccaat tatttgcaat gagatccgtc aaagtgttca
     1741 ttacgagggt catggaaaag tataggaaac taactgcttc ttccgacgat tgaatggaga
     1801 aatgattgat tcttaataat tccagccatt tttcgttcat tggagaatca gccgatagtt
     1861 ctctacgttt ggttgtctgc tgatcatttc gctgggttaa caagtataac ctttgccaat
     1921 agatactttc gtgatctaag tcgcagcacc actctaaata tttttcgatc cccaaaaaga
     1981 aatgttgttt gttttcgcta atgaatttta agatatcttg atggactatg tgagaactgc
     2041 ttttgatcgt atacaaataa gcatcttcca aatcttgaaa atatttataa aaagcaccgc
     2101 gcgacatccc catcttgtca acgatttctg agactttgac ttggctgatg tgctgtgagc
     2161 aaaatatctc cagtaatagg tcatcgattc tctgtttctt tgcgttcgat aaattgaaaa
     2221 aagtttcttt tggcatccac atctctcctt tatctctgat tatacgtgga tagcccttca
     2281 ttgtcatgaa ggattcattc cttctttttc tcttcttctg tcaagtaccc atcttccatt
     2341 cgataaacgc gatcactcca ttgaatcata cggacatcat gagtaaccat aatagtcgct
     2401 ttttgtttct catgagcttc tttaactagt aatttgacga cttcatatgc atgatctgta
     2461 tccaaactag ctgttggttc gtctgctaag atcaagcttg gctcattata taaagcgcga
     2521 gcaattgcca ctctctgtct ttctccacca gaaaggtctt taggataact atttttaagt
     2581 gactggatat ctaaagattg cagcaaggaa tcgatttttg ttgaatcttc tttttttgaa
     2641 gtaacttggt caactagttc aaattgttcc tttaccgtta aaaaaggaat caaattagag
     2701 ctttggagaa taaatcctat ttctttaaag cgtaaatctg ctcgtttttt ttctggaagc
     2761 tcagttagtg agtgaccgtt gatcactatt tctccggaag tcggtgtttg caatcctcct
     2821 gaaatggtaa ggaaagtact tttacctgaa ccagaaggac caatgatgct gacgaattct
     2881 ccttccttta cataaaaatc aattgctttc aaagctgaga cctttgtatg accagaacca
     2941 aatgttttgg taactttttt catcgttaat atttttttca tttttattaa cctcctattg
     3001 ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa caatccgcct agcattgcga
     3061 caatgatcaa aatcccacta tatagcaacc attgtgacca aaaaacagca aaaggcatcg
     3121 cgcttggtaa aatcagacta gtcaaataag caaacaaaag agcaagcaga acgcctacca
     3181 ccccaacaat aaaggactgt gctactaaag atttagcgat gaagaaattt ttgatccctt
     3241 gtgctttcat tactccaaaa atagcagtct tctgaagtgt catcacgtac atgaaaatac
     3301 cgaccacggc agcaaccact aagaacaaaa agtatatcat cgcatttaag gtcatatttt
     3361 gggcagaata gccaggaata ctttcaatga atgtcccaat agttaataac tgtagaccct
     3421 tcgatgcttt ttctttcgaa attttagctg aatcctttat tacgatccca ttgatcggct
     3481 gttcttcttc agaagcaaac ggctgttgtc catacttcaa agcagtccac gtatctaggg
     3541 acgcatagat gacgggagaa actgtgtaat aagtttcagg aaagataccg acaatcgtca
     3601 aaggatcgtc gtaactgcca attttgatcg tatctccaat tttatagcct gaatcagcca
     3661 gattttgaga gatgatgatt tcattctttg ttttaaattc atgccctttc gtgagttttg
     3721 gtaataaaaa tgcttctttc gttgtgccga aaactgtaac attttctttg ttttttcctt
     3781 tgattgctcc gctgtacaat ccgattggtg ctttctctcc tccttcaatg tatttcagat
     3841 ctcctcttgt taattgtgaa gctgcaaata cttgatttgc atcctccgac aaaacaatct
     3901 cttgtgcgtc ccaatcatcg attgcttttt taaactcttc tgaaagtcca ttcgctagtc
     3961 ctgacaacat gaatacgaca tacgcaatca agaacataat accgacgatc aagccatatc
     4021 gtaatttaga atagcgaatt tctttgatag ctagaaacat ttgtttttcc tccctatgat
     4081 agattgatag tgacaaaatg tcacttgttg aatagtgtaa ctctaaagtg acaatttgtc
     4141 acttgtgttg tttttttctt atcaataaat tgtctaagac atctttcgat aaaaccttaa
     4201 catgagtcat aaaaaattta tttaagcctt atttatgctt tccatgataa actaaagtga
     4261 ccaaatcaat gaggtgggaa aatgattaaa ctattagtag tagaagacga taaagtatta
     4321 tccaataata taaaagaaat actcgctgca atcggcgaga taacacaagt ttatgatggt
     4381 agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc ttgcaatctt ggatttgatg
     4441 atgccggaaa tgaatggata tgaagtgtta gcaaaaatac ggaaagaaaa aatacagact
     4501 ccagtattga ttttgactgc aaaagatagt ttggaagata agattgaagg atttgaacga
     4561 ggtgcagatg attatctaac aaaacttttc tatcgagaag aactgatcat gcgcgtgaaa
     4621 gcattattga aacgctcgct aggtctcttt gatgaaaatg ttctgaagta taaggatatc
     4681 agttgtgatt taaaaagcaa tgaagtcttg tataaagaac aagttttgcc catacaagga
     4741 aaagagtttg aattgctcgt ttatttttta caaaataagg gaatcatttt gacaaaagag
     4801 cagatatttg accggatctg gggatttgat tctgatacga cgattacagt tattgaagtt
     4861 tatatgagca acttacgaaa acatctgcga agtactggat tggataaaga aatcaaaacc
     4921 ttacgtaatg tcggttatat actgcaaagg gtgtgatgca atgaatggag atattacacg
     4981 aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca tttgctttta tcttttttct
     5041 tttgggattc attactttac agatattaaa ccaatctgca tacagagaaa cagatctgtc
     5101 attagagaaa ataaattttg attctcgaac agtacaatta gagatacaaa gatatcagca
     5161 aggcgatccc tttttaaacc cgactcaaac ggaacaaaac agaacaaaca tggaacagcc
     5221 attaatggaa ccatcaaatg accgttttaa cactcaagtg atcttgtggt ctaaaaatgg
     5281 tgagatattg aataaagcag ctattggtgg gcgtttcaac caaatcaaag aattaaaaat
     5341 ggatacgaaa aatctgaata ccatccaaaa tattgaatta aatgaagaca gtggctcatc
     5401 tgccctctct ttccgttcga ttacgaaaaa agcggagacg aatcaagatg gcgttgccta
     5461 catccaaatt ttagaaaata caaatcaaat caagaattca ttagcgactt ttcgcacgat
     5521 tatgattttg tgtatgattg ttttttggat tctctctatt ggtatcagtt attacttgtc
     5581 tagtctaagc atgcgcccta ttattctttc ttggagaaga caaaaagaat ttgttgagaa
     5641 tgcttctcat gaattgcgca caccgttaac gattattcaa aatagtttgg aacatctttt
     5701 taccaagcct aatcatacta ttattgaaga atccgaaagc atcgctcaag cattgtcaga
     5761 gacgaggcgc ctcactggat tgacttcgga tttgctgaca atcgctcgga atgattctga
     5821 tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa tatatcgaac aacttgttaa
     5881 accatttcaa gagatggcca tcatagatgg aaaagagttt attctccata acgatgcaac
     5941 attagaagta ccaattgatg aaaaaaaaat ccatcaagtt ttggttattt tgctggataa
     6001 tgcattaaaa tatacgaatc cagaagataa gatcattttg gattcatctg ttaaccataa
     6061 agaatggctg attcacgtaa agaatactgg cccttctatc agtgaagaag atcaagcaag
     6121 gatttttgat cgattttatc gggaagatca atctcgggcg aaagagactg gcggatacgg
     6181 gcttggcttg gcaattgcaa aacaggtaat cgaacagcat catggacaga tttctgtatc
     6241 caattatctt ccacaaggag tcgattttaa agtccaattg ccgttgaagc ctaaaaaata
     6301 atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc acagcccttt tttgttgcga
     6361 ataaacggtg ttccagaagt agcttcttcg gaaataagcc gaaatttctt taagctgacc
     6421 acttctgtca caacctcttt agcttacgct tacgattttt tattgcggtc ttcaatgtgt
     6481 acatcaccgc ttgtgtcaat atccaattct tctcggctaa catcttctgt cactttttca
     6541 acatcttcat gttcttcttt atgaatccca acttcttcac gaataactgt atgtttgctt
     6601 acatctactt gctcttcttt gattgggata gtaatcgtgt catcttccaa tttttcgtct
     6661 gttccctgtg aagattgatc tgttactggt ttgcgttcga tgacgatttc ttcacgtttg
     6721 accggtactt ctaccgtttc agtatcgttg accacatgtt tgtggatgtc gacttctcca
     6781 gttgtcacat catgtgtatt tacatctaat ttttcttctt ttagttgaat tgtatcatca
     6841 tctagatttg gtttgcgatc gatgtcttca gtcttgcttg tacccattgt atcagtctcg
     6901 ccaggtacgt ccatcggatc agttcctggt gtcatattca atggatccgt tgtattttga
     6961 aagcctgtcg tgccacctac gtccattgga tctgttccag tgccagtttc taatccagtc
     7021 cccattgtgc ttgctgggct aacagtatct tgtgtatcca ttgtatcttc tgttgtttct
     7081 ggctgacgat agccttttac tgtgatgaca aattttccat cagaaatatc tcctctatat
     7141 tgatataaag gatcattttc tggtgtagcc gtctcagaat cataggtatc aaaagaaaaa
     7201 gtatctttta ttttttccca taaagaacgg tcttcttcac catccatccg attttcatct
     7261 gtttcaacat ctataccaga caatccgtca aatcctagtc tagatgctgc ttcatcagta
     7321 gtataaataa cgatatcatc tcgttcatac ccttcgttac gcaaccgttc aattactgat
     7381 aatgcttctt ccctcgtact atagcttccg atgaccgtta aatttttctc ttccatgtaa
     7441 attcctccta gcaaatagat ttatgattgg tatattgtta cttataaatt tattctatag
     7501 gagacgattg ttttctacaa atatttagat tggaatttga ttaaaacaat ttaattgaaa
     7561 tttgaaaaca tttatgttaa tgaaagtcat attctaataa aatgtcattt gtttctatat
     7621 gaatttttat gaaaaatttc atataatttc cactcgttgt gatggtttaa tttatccaat
     7681 ccatcaataa gcaaacaact tatataaaaa aggattaatt ctcaatataa ttgctcctac
     7741 catagaataa atcttcatgt aaagtaatga agtaccaact atttctaata cttctttaag
     7801 aattatccca acaatatcgt tagacaaacc attattgaat aaatggagag ccaatcgatt
     7861 ctgaaaaaat gttttctaat ttttcggaaa taaaagaaag agcctcctgc cattagaacg
     7921 aaccaaatcc atttcattgc tcatgaagta accacatagc taaaaagagg ataaatcaat
     7981 taaaagatca aaagaatgac tgcactaata aaataactaa aaatgtatct tcttacttca
     8041 tatcttttag ctagtactta gttaaaaatt tgcttcctaa gatcaagaca aactgtctat
     8101 cttcatttga tgaaatgcgg taaaatatag aaaatgaaca ctattggagg gaatttgtaa
     8161 tggacagcaa aactagaaaa caactgcaag aaaagttaaa gcaacgtaca cacttgctcc
     8221 cttccgtctt tcaacagttt ttagaagaaa atcgttcttc cctttcatta ggcagtcgct
     8281 atgaatacgc aaaagatttt catctgttta ctgattattt gagaaaagta ttttctgaag
     8341 aagtggatga tcagctgatt atccatttag aaaaacaaga ttatcagaat ttcttagcta
     8401 aaatctatcg acatactcgt tcgttccaaa ctacagcgaa ccaagaaacg gagcaattgt
     8461 ttgaaaatag ctattacggg atttcaagga agcaatattc attaaatcat tttttacgat
     8521 ttttgtatca aaaaggactg attgaagaag agatttctct tttgggtaca agtttaccgg
     8581 aaacaaccaa agcagctccg cgatatatcg atgcagaact tttcaaagat tttgagcatt
     8641 tgtttatccc gattgaaggc tttcaaacaa gcagagaagc tagctttgaa gaaaagaata
     8701 gaccgaggaa ccttgcaatt actgccattc ttctgtattg cggattaaag atccatgaag
     8761 tagtagaact taaacgtaaa gatgtatctt tgaccaagca gattcttaat ctgaatagaa
     8821 aagaaaacag actgaataaa gtaccaatac caaaagaagc gatgccattt ctggagaact
     8881 attttaagtt aacagcacaa aatacggacg aaaattcttt tgtttttcgt tcctcacatg
     8941 atcagcaaat ttctcctcgg actattcgcc aaattttgag caaaattaca gtatataaaa
     9001 acgtatcgcc agaactatgc agaaaaacat atcgttatta tgcagaacta tatttagatg
     9061 atgctgacgc ggtaaattat ttatgtggta atcgatacct ctcatcccat tcttttcaag
     9121 atatctggga aaagatggtt gatttttctt atcacgaatt agcggcattc gttcgggttt
     9181 cttccatata aaacaaatgt atgatctttt tttcacaata aactatgtta ttatataaag
     9241 gattatgtta cgattatctt gaatttgtat attacttaac aaaagataat ggagtgtgaa
     9301 tgatggagac cataacgtat tcccctttga acttatttac gaattttcaa gaagctgcag
     9361 ctaacttttc cgagaccccg attatctttg atgagccact ttcagctttc ccagaattag
     9421 gacttgaaac aacttacaaa aaatgctgcg aaagtgtgtt gaaacgggct tatcaattgg
     9481 ctcatcttgg agtgaaatcc ggagacaaag ttattattta taagagttcc gcttttgaca
     9541 cataccttct agctgtttct gtgtcttacc ttggtgcagt gcctgtcatg acttcttatc
     9601 atctaccaac aacaacaatg gaagtattta tcgatcgttt agaagaccct ttcattttat
     9661 ttgatgatga gacaaaaaag cgagtgagag aaatttcaaa tggaacaaaa tcaaaacaga
     9721 tt
//
LOCUS       DAFEHS010000088         9665 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFEHS010000088
VERSION     DAFEHS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.093"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..650)
                     /locus_tag="LP327_002392"
     CDS             complement(<1..650)
                     /locus_tag="LP327_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL1726482.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="LP327_002393"
     CDS             975..1391
                     /locus_tag="LP327_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL1726483.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="LP327_002394"
     CDS             1388..1858
                     /locus_tag="LP327_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL1726484.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="LP327_002395"
     CDS             1872..2645
                     /locus_tag="LP327_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL1726485.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="LP327_002396"
     CDS             2659..3471
                     /locus_tag="LP327_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL1726486.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="LP327_002397"
     CDS             3525..4490
                     /locus_tag="LP327_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL1726487.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTIVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="LP327_002398"
     CDS             4606..7329
                     /locus_tag="LP327_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL1726488.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="LP327_002399"
     CDS             7342..7974
                     /locus_tag="LP327_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL1726489.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9665
                     /locus_tag="LP327_002400"
     CDS             8873..>9665
                     /locus_tag="LP327_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726490.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3261 a         1477 c         1884 g         3043 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agcgacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactatcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtatt
//
LOCUS       DAFEHS010000089         9588 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFEHS010000089
VERSION     DAFEHS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.094"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(70..540)
                     /locus_tag="LP327_002401"
     CDS             complement(70..540)
                     /locus_tag="LP327_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL1726491.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWGKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(537..779)
                     /locus_tag="LP327_002402"
     CDS             complement(537..779)
                     /locus_tag="LP327_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL1726492.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(1325..1855)
                     /locus_tag="LP327_002403"
     CDS             complement(1325..1855)
                     /locus_tag="LP327_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL1726493.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(2037..2693)
                     /locus_tag="LP327_002404"
     CDS             complement(2037..2693)
                     /locus_tag="LP327_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL1726494.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(2820..3293)
                     /gene="dfrF"
                     /locus_tag="LP327_002405"
     CDS             complement(2820..3293)
                     /gene="dfrF"
                     /locus_tag="LP327_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL1726495.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(3712..3930)
                     /locus_tag="LP327_002406"
                     /pseudo
     CDS             complement(3712..3930)
                     /locus_tag="LP327_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3961..5174)
                     /locus_tag="LP327_002407"
                     /pseudo
     CDS             complement(3961..5174)
                     /locus_tag="LP327_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
     gene            complement(5515..5757)
                     /locus_tag="LP327_002408"
     CDS             complement(5515..5757)
                     /locus_tag="LP327_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL1726496.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(5789..6346)
                     /locus_tag="LP327_002409"
     CDS             complement(5789..6346)
                     /locus_tag="LP327_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL1726497.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(6359..6547)
                     /locus_tag="LP327_002410"
     CDS             complement(6359..6547)
                     /locus_tag="LP327_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL1726498.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(6560..7126)
                     /gene="amaP"
                     /locus_tag="LP327_002411"
     CDS             complement(6560..7126)
                     /gene="amaP"
                     /locus_tag="LP327_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL1726499.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            7543..7716
                     /locus_tag="LP327_002412"
     CDS             7543..7716
                     /locus_tag="LP327_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726500.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            8356..8829
                     /locus_tag="LP327_002413"
     CDS             8356..8829
                     /locus_tag="LP327_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBL1726501.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            8838..9065
                     /locus_tag="LP327_002414"
     CDS             8838..9065
                     /locus_tag="LP327_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1726502.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2944 a         1870 c         1393 g         3381 t
ORIGIN      
        1 aaaatctgta tagaaaaagc agtcaatcct aagactaact gctttgtgtg tatggatata
       61 aaattgcttc taatcttgaa taggtatcca aatttcaata actgaattat cactattcca
      121 atgaaaacga taatcgtatt tttcaaagtg taggataata tcttttatag gaatatacga
      181 tgtactagga ataatttctt cataaatctt gccataagtt tcatatattt tatccatagc
      241 accaatatgc tccacaacca aatatttata agacggtatt tctttataca gaaagccctc
      301 tggggtaatg gttctttggg gaatagaaca gaaataataa agttttccat ttcttctttc
      361 cataaaagca tattttaccc aatttcctga ttgtgaaaga tacgcttttt tcaaactact
      421 gttaaacttt ccccaaaact gtgtactaat ctgaatattc tttttttgat aattagtcag
      481 ttctacttcc tgcccaataa ctgcaaatgc gtttaattcc tttatagaaa aatttatcat
      541 tttaccttgt gagcagtgat aacggtataa ctgtacgcat ttaccgttat aatacaatta
      601 tcaatcgtta tataccagtt ttttcctttt cttgtgattt ccgcattaga aaggctaatt
      661 ttttctttgc accattcaat tacattttct acattcaaag ataagttctt tttaattcta
      721 ataacgccaa gttcagttgt gtgaagtctt cctaagtttt ccagtaattc attttccata
      781 ttttgatacc tcatttttca gcaaatttat ttgtatgttt tataccccaa ttataacatt
      841 ctcatatcag cactacaata aaaataatta agcaccaata atcttattga acatctccaa
      901 ccgtaaatct ttcacaccat cagtgataaa cactgagctg acagcaatta cgacaactac
      961 cataattgcc tgcacttaaa tttctgcaaa agaaaacgac aatcaatttc ttattttttg
     1021 aaatcaactg tctttctcat atttactatt caattttcat agtgatatag gtacaattta
     1081 tatcactact gtaaaaatgg ggttttgcct tataaataca gcgttttccg cttgttagtg
     1141 tatagtgttt cttgtttttt gtgcccccct tgttagataa cgggggcttt gaacggctcg
     1201 caagctcgcc gaaccgccga agcgaaccgc ccccttgcaa gcatggcgga aaaatcttaa
     1261 ggattttccc gccaacgtgg tcggatcgct ccgtcggttt aacagctttt ctgtttctct
     1321 ctagttatct acgcatataa atcaaatcgg ttattccgtt ataagtctgt gttttacaaa
     1381 gccttaactt tatttccctg tctatgttgc ccaaaagacg aaccccattt cctaacagag
     1441 taggaataac agaaatgtaa tatttatcaa tcatattttt gcttaccaac tgctgaacaa
     1501 gatttgctcc gccacaaatc caaatatcct ttcctgcttc ttgttttagc cgttccaata
     1561 ataaaacagg attttcactt gtaaaatgaa tttgttggga agaattgcac tctttatgtg
     1621 ttaatacata agttgtaaat ttattatata cccattcttg cggagaaagt tcagtaacaa
     1681 cctgatgaga agtattccac cccattaaga cagtatctat atctttggta aattctgtat
     1741 aagtgtcgat gttttcattg tcatttccat gaccatttag ccaagctact ccaccattac
     1801 cgtccgcaat atatccgtca aggctcatgg caataaataa aactactttt ctcatttttt
     1861 ctcttttata aatacaaaca ggaatttatt tctctttttt actcataagg gaacaactga
     1921 ttgctacaaa taaacccatt aaaatccatg gaaatgctgc tcttataaaa tcactaacca
     1981 tactttattc ctccttaaat tccaatttcc ttagttctac aaactgaaat ttttgcctat
     2041 gaatgtatct tgttctttat acgcaaacag gcttctgtgt gcataatcca atgtcttgaa
     2101 aacggcattt gaattagtcc acaaatatct ttattacacc aataatcaat cagccaaatt
     2161 gctttttcat tgtcgtttac tacattcaac gattctaagt atccttttct ttctttcggt
     2221 atttttcttt tcaattcatc ataagataac cgttcaagca ttttctcagt gctttcccat
     2281 acttcgaaaa tatataaata taattcttcc agattaagct gttttgaaaa gtctgcaatc
     2341 tgctgtttca tgagttcatt tcctgttgta atgataggtg aattgatacg ctcttgataa
     2401 ttgcctgcaa aaaatacttg ttcatcttca tttatcactg tatgcacaac tatatcttca
     2461 atgcggaaaa tatgccaaat agaataagca atcgtcttac tatgataacc atcagcgttt
     2521 atgaagggga ttgagttaaa atcttctctg cataattctt ctttgaaaga tactaaggtt
     2581 tgtattaact gacttcgtaa agtaagtaaa gtatcaatac ccctcttata agtatctttc
     2641 ttttttattt gtgcctgcat tgttttatta agttcagacc attccttatt catattcaat
     2701 cctctctcaa attctgattt tatcgcccca tacaaactgg aatttgtctt acaacaattt
     2761 atatatgcat aaaaagcaat atatttatga aaacacccta aatccaaaat ttactcaatt
     2821 cattttttcc ttacataaaa tgttctcgta tatttcactt cctcaccaag tgtttcccct
     2881 atcaaaactt caaaatcatt gatatcaaat tctggaaaga atgtatctcc atcttcaata
     2941 tttaaatcta cttctgtgat atacatttta tctactattt gcaaagcttc cttaaatagt
     3001 ccatatccac cagatatgta tacatctcgt cctttagcca ataataatgc atcttctaat
     3061 gatttaactg aaactaaatt atctccttga tactctgttg tggtggaaac aacaatattc
     3121 attctattag gcaacggatg accgatttct tcataagact ttcgccccat aataaccaca
     3181 ttacccgttg ttaactctct aaattgcttt tgttctccct ttattttcca tggtatatta
     3241 ccattcttgc ctataacatt attctttgac ctcgcaacaa tcaaacctat catctatttc
     3301 atcctccata atatgatgtc gtacctgtga ttccatcaag tgcaccacat cttaacaaac
     3361 tcaaaattat ctcgaactaa ccagctcgac aaattccttt tttgtcttca tctgcattat
     3421 atcatattgt tataagcatt gccatttctt ttctgctttt gaaaagttac ttatcagcct
     3481 atgttttgta aagggtgata ttcagcattt ctcacgcaac aagcccttga caaaccatag
     3541 actaataagt tttaaggtaa tcaagcagaa atgaaatagt ctgctgtttc acggcaacaa
     3601 aaaagagata ccaacacact tatgtagtat ctcttagtct agtagcaata ttcttttgtt
     3661 tgcaagcagg taaacatggg ctgtttctta atatcttttt gtttgggaaa cacctttttc
     3721 ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa accatagcaa
     3781 accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg tgggatattc
     3841 atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaacg ttaatttttt
     3901 tgcaggaatt ttcgttttgc tttgttgcat gaatcttcct cttttctatt tgaatttgtt
     3961 tcaattgagt ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt cttcttttgt
     4021 agtagtgatc gttgtaaagt gcatcgccga ttttaaaatc actgttgtca gcacataggc
     4081 atacacggca gtaatgatga caccaatcac ttgaatccct aagaagtacc agctgcttgg
     4141 attcgttagt aaatggctgt tagatgcaaa gatccctatc agaatcgtac ctgtgaatcc
     4201 gcccatgcca tgaacacccc acacatcaag tgcatcatcc cattgtttct ttttacgaaa
     4261 atcaacagca gcatgacaga cgatacccgc aatgatccca atcagcatgg cactttttgc
     4321 ttctacataa ccggcacaag gcgtaatggc tgccaatcca gcaacggatc ctgttagtac
     4381 gtcgacaaaa tcaaagccat ttccttttac ttttgcaatg atcagccaag taatcatcgc
     4441 aaatgcaaga gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat ctgctcgaag
     4501 tgcaccaccg gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac cgatagcagc
     4561 tgccataaga ctgctatgct gacttctttc tcctaaagga atctttcttt gaccaagtgt
     4621 gaggatacat gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc caccagcgaa
     4681 atctctaaat cccatctgat ccaaaaatcc gccaccccat atccagtggc aaacagggaa
     4741 ataaatcagc agattccaaa aaataacaag tataatatag ccttttaaat tcaatctccc
     4801 tgcaaaagct cctgtcatca aaggaacggt aatcacgata aacatcagtt ggtaaagaaa
     4861 gaacattaaa aatggaatcg ttgcaccatg aaacatgttg gggaagcttg taacatgtct
     4921 catcaaaaag aaatctgccg ggtttccaat aacaccgccg atatcccgtc caaaggccaa
     4981 tccgaatcca ccaaataccc acataatcgt tgttatccca acggagagaa aagattgaga
     5041 catgatcgtt agtatattat tcttccgtgc taatccgcgt aaaaaaaagc tagaccagga
     5101 gtcatcaggc aaaccattgc tgtacataaa atcatgaatg ctgtatctcc tgtattgata
     5161 ggtaatgtat tcattttttt cgcctccttt ctacgattac gagtataaaa gaaggtgtag
     5221 ttgttttttt cttctgtttt tttgaattaa tacttgcatt tataaaagac agacaattaa
     5281 taaaagcttg atgtgttgtg ctctttttcg tttttcacac aaataaagac aaaaaataaa
     5341 cggtcttctt tttatagaaa accgtttatt ttcgtctttt tttattttta aagatatttt
     5401 ttcataagga ataaagaata cttcatataa aatatataaa aaagaaccgc agcgagtgtc
     5461 gctacggttc tcaaatttgt tcttcatttc aacataagtt tttcttcaat ttgcttataa
     5521 tttacgtgca aagaatgaaa cgactaatac taaaatgatt gcaccgatca atgatggaat
     5581 gatagccatt cctgcaattg ttggtcccca agttcctagt aaagcttgtc caattgctga
     5641 acctactaaa ccagcaatga tgtttgcgat gatccccatt gatgatcctt tattcgtgat
     5701 tgctcctgca attgctccaa tgataccgcc gacgattaat gcccaaataa atcccatttt
     5761 aaattcctcc taactgactt cttacttctt attgtacacg tggttcgtta ttttctttga
     5821 ctttttgtgc acctttgcct acagcagatt ttgctttatc tgtttggcgt gaagcaaatt
     5881 gtcctgtgga ttttgctgct tctgttactt tgtcttgtac tgtttcttga tctttttggt
     5941 attcttcttg tgttttgatg tctgcaacat tcacattcac ttcgataaca tctaaatgtg
     6001 tcatattgtt tacttcagta ctgatcaatt ctttgatttg gttgtaaata tcttcgatgt
     6061 ctttaccgta ttctacgatc acatctagat ccacagctac ttgtttctta cctacttctg
     6121 tattgatacc tgctgttacg ttatctgtgt tgactaattt ttcagccata tttgagaaaa
     6181 atccgccatc taatgtcaat agaccatcta catctttcaa tgcataaccg atgatttttt
     6241 ggatgacttt gtcttcataa gtcaattcac cattcatcat gcggttttct cctttttcat
     6301 ttgccaaaac tgatccattt gttgtattca tgtttttgtt ttccatcttt gtttcctcct
     6361 atgatttaaa aaattgatca actattcctg actgtttcaa ataaaaaccg attcctgcgc
     6421 caattgctgt catgattatg atcaacaatg tcttgaaaaa tccaatcgtt aaccataaaa
     6481 cagctagcag tagtccaaga cccccaaaga taatagggag cttgtatgct gaaaatagtt
     6541 ctttcattat ttggccctcc tattccactc gagagtgctt gtagtctctt gtatcttctt
     6601 gttcttcata accgctgtat gtgactgcca ctttgaccgt ttcttttgtt ccgagtactt
     6661 tagttacttc ttcttcgata tcttgcatca atacacctgt tttccctact aatgaagaag
     6721 ttcgtttcaa gtctccgttg acatggatat ggattcgatt tttcgttgaa cgtatccgta
     6781 ctttaggtga atgaatgaac tcttcttcgt gtaaatggga acgtaccaac ccttcgatcg
     6841 cttttttgtc gattgttagt ttgccatctt cccgtttcat aacgaaagtt cctcttgact
     6901 ttggatagaa aagaacgact agcaagatga taatagcaag aacggcaaat actacagctg
     6961 cccagaaaat atattgttgc atgtagtaat tcgttaaagt aaagaagcga aatcgttcca
     7021 attgttcagg cataatatac ggcgcattga tcaacgcaat cgctagaaat actgaaagta
     7081 gcagtaaact catgataaca ccgattattt ttaacccctt attcattttc cacctcctat
     7141 aaacttattt ttaccctgta aataactcta ccgacaatca cagtatagat agcgaacagt
     7201 tagcatagca tcagtaaata aagcgctacc aaacattgct ggatcataac ggcgttttat
     7261 tgccagtctg atactgaaat aacaattgct tacttattct ctatttactt ttgattgctg
     7321 gctatactta aaggatatta ttcaagatta aaaattgcaa agattatgct cagttattat
     7381 tacaagattc tatttattta ttaaattttt tcggagaatt aaaaaataca ataaataact
     7441 ttagttactt taatgcgttc ttactttatt tttatattat catttttctt attcacttct
     7501 ttggtttata ctattcttat aaataaaaac gaggtatgtc ctatgatgaa aagattgtac
     7561 tattcattga tcataacgat tggctatttg attgtatcaa atctaggaaa tatggtattt
     7621 ggtatcagta aagaattcag ttggactact acattatggg aatctctatt ctttttcatc
     7681 ttcgtatttt tgttacaaaa ttatcgaaaa aaataaacat gtgccatcta tctgttggca
     7741 catgttttag gatttatggt actttttatt cggcaatcca ttttattttt ctacttcttc
     7801 ttaatacctc taacactttt gatttataaa ctaatccaat gcatctactt gcgcttatta
     7861 agctatttca gctagatagt accaccccat gcgtcaatgc ttgattttcc ttttgtataa
     7921 cattgtataa gcaataaagg acaaataagt ggtgattaca ccaaaccatt gaataagaaa
     7981 aattctgata cgtaaataaa catgaacttg aaaaaattaa tgaatcggtt agctactacg
     8041 tagttgtctt ttttctcata cagttctgtt aacttacact tctcttcgct agataataaa
     8101 aaatcctcca actgtattgt ccaatataat cccaggcatc taaactagca ttatagaaga
     8161 gacacacata gaatctaaaa taactggttt taaatgtttc ttacaatcaa aaaaagctgt
     8221 taaatgcact ccacaccttc atactctatc attttttata aacgatatat ttttattgtt
     8281 tttcgataaa aaatagttgt tttacaataa ttattctgct atattttagc tatcaaagat
     8341 aaaagaggga taaaaatgaa atttaaaaca ttgctattaa aatttgtcgt tttaggtata
     8401 agtgccgtat ttattttgtt cggcgtactt ttttatactc agatcgctac aagcgaaaaa
     8461 ggattcaatt ttgattggca gaccacatta tttgtttctg tcatttactt tgcccttctc
     8521 ctagggatcg ttattgctta tttcttattg aaactattga aaaaaatcga agataaccaa
     8581 gctttttcca ctcaaagctt aaacatacta aaaaaaatcg aacgaaccat tttgtttatt
     8641 ggtttcgcct tttttggctt acttcctaaa tttatgaaag tagcggattt agacgatgct
     8701 ccaggtttga tgttggtagg aatcgctgtc gtattcattc cttttgcagt atacacatta
     8761 acaagcgtgc tggaaaaact gcttgaacat gcgattttac ttaagacaga tcatgatttg
     8821 acagtgtgag gtgcgacatg gcaattatcg ttaatttaga tgtagtatta gctcaaaaaa
     8881 aaatgagtgt gactgaattg agtgagaaag tgggcattac gatggcaaac atctctattc
     8941 taaaaaatgg aaaagcaaaa gccatccgtt tttccactct cgaaaaaatc tgccaagttt
     9001 tggagtgtca accaggtgat ttgttgacct ataaaaatga ctcgaatact ataccagaag
     9061 aataaaataa agtagaacaa acagtaaata aaaaataatc tgaatgatag atgtgtgaga
     9121 cttttagaag ttatttacgt ctattattca gattttttat ttcgattgaa taccgtttta
     9181 tctataaata agatatgtac tgactcatac aaagggggcc ttttcttctc catgtactat
     9241 cacttttcct gatacaactg gtttcctgtt acgttgtagt tggatctgct tttagtataa
     9301 tactttattc attccaccag actgatagca gtttgagcag tctaaataat catgtagtat
     9361 tacaggccat tctgtttcat accttgaagt gcttaaaact tcagctaatg gcccattgtg
     9421 ttaactctta ctccattctt ttttagtaag ctcccatata tactgtaaag ccttctatat
     9481 attatgtcat gttgcttttt aacatttact atgtagtttt gataaaatga tttgaataac
     9541 agtgatggac aattcttttt tatttttaac attattttga taataatg
//
LOCUS       DAFEHS010000090         9445 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFEHS010000090
VERSION     DAFEHS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.095"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(131..973)
                     /locus_tag="LP327_002415"
     CDS             complement(131..973)
                     /locus_tag="LP327_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL1726503.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(1010..2476)
                     /locus_tag="LP327_002416"
     CDS             complement(1010..2476)
                     /locus_tag="LP327_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL1726504.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(2532..4376)
                     /locus_tag="LP327_002417"
     CDS             complement(2532..4376)
                     /locus_tag="LP327_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL1726505.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            4978..5928
                     /locus_tag="LP327_002418"
     CDS             4978..5928
                     /locus_tag="LP327_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL1726506.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(5973..6606)
                     /locus_tag="LP327_002419"
                     /pseudo
     CDS             complement(5973..6606)
                     /locus_tag="LP327_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(6866..7414)
                     /locus_tag="LP327_002420"
     CDS             complement(6866..7414)
                     /locus_tag="LP327_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726507.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(7415..8272)
                     /locus_tag="LP327_002421"
     CDS             complement(7415..8272)
                     /locus_tag="LP327_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL1726508.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(8558..8845)
                     /locus_tag="LP327_002422"
     CDS             complement(8558..8845)
                     /locus_tag="LP327_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL1726509.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(8835..9164)
                     /locus_tag="LP327_002423"
     CDS             complement(8835..9164)
                     /locus_tag="LP327_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL1726510.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(9241..9387)
                     /locus_tag="LP327_002424"
                     /pseudo
     CDS             complement(9241..9387)
                     /locus_tag="LP327_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         2848 a         1906 c         1445 g         3246 t
ORIGIN      
        1 agatttttta ttatcttacg ttaatccttt ttaaatgtct cttatagata acaaacagct
       61 ggaaatcacc ttgtttttac tataatccat ctctaaagat tttaaaagca tcaatagtat
      121 tactgagcgc tcacccatta gcatgtttca tcaaccgatt gatgtggaca cctaggtata
      181 caacttcatc gttcggaatt gttttatgat aaactttttc aatgtagtct ttgatcttag
      241 ctgcgctttc catggcagac gggtataaga tccacatttg attgtagaat tcttcctcac
      301 catcatccaa caaagcatca gaataatatc tttctacaaa gaaacgtaca tgtgtaatga
      361 agcgggcata atggatcgaa ttagtatcga tatctttctg caacgaatac cggaccaaat
      421 tgacgatccc gccgatcatt tttgcatact tgaatccagc gccgctatca ggatcttccg
      481 attgtgcatt gatcaaatgg aaagcaatgt ttgaggcttc ctctttagga agatcaattc
      541 catattgctc gttcaaaagg gtgagtgcgt acttcccaat ttgaaattct ttgggatagt
      601 agatttttat ttcccagtac aaacggtttg caatatttac ccctttttgt gcgcgttcaa
      661 ctgcaaaatg caggtggtct gttaaggtta aatagactcc aggattcagt tttttatcta
      721 atcctttttc tgcttcaaaa acgatagtac gagtgatttc aaaaaattca ataggtattt
      781 cttcaactaa ctctaatagt tgtgaagatt tcttttcttc aaaaggcaga aatattttat
      841 ctactttatc tcctgatatt tcatccccag ctttctgtcc aaagcctatc cctttaccta
      901 atgcaatcat ttctttgcca ttttcttcaa caagtaccac acttgaattc aatacttttt
      961 ttattgaaat cactcattat ccccctatcg ctgcttaaaa agatcgagac tactcgaata
     1021 aggatgcacc gtttgtttcg atcacttctt tgtaccagtt gaacgaatcc tttttcaagc
     1081 ggttatatgt cccatttcct tcatcgtctg catccacata aataaatcca tatcgtttag
     1141 acatttgaga agttcctgca ctaactaaat caattggtcc ccagctagta taagctatca
     1201 aatctacacc ttcatcgatt gcttctttca tttgttggaa gtgtgcttta aagtagtcga
     1261 tgcggtacgg atcatggatt ttaccatcta ctacctgatc gactgctccc ataccatttt
     1321 ccacaatcat caacggtact tggtaacgat cataaagttc gatcaacgaa attttcaaac
     1381 cttttggatc gatttgccag ccccaatctg ttgaaggcaa atgaggattc ttaaccccta
     1441 aaacagtatt tcccggtgtg cgttctgcgt ttggatcagc tgattctgcc atggacatgt
     1501 aatagctgaa agaaacaaaa tctacggtat gtttctttaa aatttctcga tcttcatctt
     1561 ccattttaat ctgaattccc cgattttcca aatccttttc gattagagca ggatattctc
     1621 ccttaacttg aacatctgca taaaaattat tttctaagtt ctttttcaag gtcagctcaa
     1681 cgtcttctgg tgcgcaagtt aatggataag tcatcaattt cgttaacata caaccaactt
     1741 ggcttcctgg aattttttca tgagcatctt ttgtcactaa cgcactcgca acaaattgat
     1801 gatgcaatgc ttgatagata tcttgagctt cttgaccttc tgcgcttttc tcttggataa
     1861 ttccagctgt tgtaaatgga tgacggtgga tgctgtctat ctcattaaat gtcagccaat
     1921 attttacgta tttgccgaag cgatcaaaac aaacgttagc gaagcgaaca aaatcatcaa
     1981 ttactcgacg ctccacccag ccattatatt taactgctaa agctaatggc atttcataat
     2041 gagatagtgt cacaatcggc tcaattccta aacgttgcat ttctttaaag acgttttcat
     2101 aaaatgcaac ccctttttca ttcggttcca gctcttctcc tgtagggaac aagcgagtcc
     2161 aagcaataga gaggcgcaag gttttaaagc ccatttcggc aaataaagct aaatcttctt
     2221 tatagtgatg ataaaaatca acgccccttc tttttgggta ccatttatca gatttgtctt
     2281 cactagcttc ttttatagac tgcagggtga catgcatatg agcagcaaaa tctttgttgt
     2341 ctaaatttgg tttataagta gccatatccg ctactgaaag acctttaccg tctacattcc
     2401 aagcaccttc tacttgattt gctgctatcg cgccgcccca taaaaatcct tttggaaaac
     2461 ttgttgtgtt tgtcattatt ctattctcct ctattccttc attcaatata ttttttttca
     2521 ctatcttgtg tttaagctat cggactgata gctaagagat cactatcatt ggttaccaat
     2581 ttttctaggt taaatgaacg gatagtgtaa ttatccttat tggttaccac acagataact
     2641 gtggggtcga atcctgcttc tttgattaaa tcaatatcaa atgcaatcag cttttgtcct
     2701 ttggctactt tttcaccaga acgaacaaat gcctcaaacc cttttccttc taactgaaca
     2761 gtatcaatac caatatgaaa aagaagttct gcaccattag ccgtttttaa accaatagca
     2821 tgcttcgttt caaataccat tgtgatttct ccatcttcag gagctactaa tatcccttct
     2881 tcaggaatga ttgctactcc atcacctacc ataccactag aaaaaacagt gtcttttact
     2941 tctgatagtg agattacttt tccattcacg ggacttgaaa gaacagcatc tgaaacaacg
     3001 gccgtttcca tcttagtttc aacttcattg acaacttctt tatctttata aagaaccaac
     3061 gctgcagcaa aagcaaccac aaatgaaatc cccgatactg caaatgccca aaccagattc
     3121 attgggtcag ctttgtcagc gaacatcgtg atacttgcaa tcccaggacc cactaaagca
     3181 aaggcttgaa gtgcaacgat tcctgcaaat gctcccccga tcaggctaga aaacataacg
     3241 gcatataaaa cacgtttgtg cagaatcgtt accccgtaaa gagctggttc agtaattccg
     3301 aataatgcag atatacccgc agaaacagcc gtagatttca attttttgtc ttttgttttg
     3361 atactgacag caaagcatgc gccagcttct gaaatattgt gtgccaatga agccgggaga
     3421 taaagaagtt ctgcacccaa ttcactcatg gatgaaacag cgtaagggat cattgcttta
     3481 tgcatacccg ttacaaccat taatggcagt acagctgcta agactccggt tgcaacccat
     3541 ccaaattgat tgtacaaaga cacaatgata gaagagaaag ctgttcctac attataacct
     3601 aatggtccta gaagcaacaa agcaatagga actgtaatca ataagctcat cataggtaca
     3661 aagaaaatac gaattggttt tggtgaaact tttgtaaacc atttttcaag ttgagcataa
     3721 aataaaacag ttaagatggc tgggaatact tgagatgcat acgctatatt atccaatcca
     3781 aaagataaga atgtagcacc ttccgccaac atagctgtca tttcaggaag gatcaatgca
     3841 ccgacagcag atactgcaac caattgattg actttcaatt tattagccgt agtcattgcc
     3901 accagaatag gcaagaaata aagcggtgcc ccgccgacca aatttaaaat ctgataggtt
     3961 tgagattggt cactaatcag ccctaagata gaagctagta gcaacaagga cttcaatact
     4021 cctccacctg caatcgcagg aattaaaggt tggaaaatag aaataataaa atctaataga
     4081 actgaaccta atttttgttt ttgttttggt gcttcttcag tatttgaaac attgtcacct
     4141 aataacgctt tcacttcatc atacacttca acaacatcgt ttccaatgat tacttggcat
     4201 tgcacatttt ggcgaacgcc cataacgcca tccacttttt ctaactggct catatttacc
     4261 ttgctgttgt cttttaacgt aaaacgtaaa cgagttgagc aatgctccaa atgggatatg
     4321 ttttcttccc caccgatgta ctgcaatact tcttctgctg tttgtttgta attcatggtg
     4381 tttccctctt ttcttttttg agtgatgaac ttttgagtcg aagtggccat cctcaacaca
     4441 aaaaacaggc agaaactaaa taaattgaca acaaggcaaa cgtgtattga aacgcataca
     4501 ttgttattca acttatttag gtcctgcctg cattaccagt cacatgcctg tttaagttat
     4561 attcaattta acatattaga atgtaaacgt aaataataaa atgatttttt aatcattttt
     4621 tcattttatt cgttcaatat agttttcgtg ttctaaaagt tttgtctttt ttctaagaat
     4681 atcttccaac atatataact cttattctct tctatcttct agcagatgtt ctgtcttttt
     4741 aagacctagt ataaaaagaa tgggttcatt ttttctttct tgcaggatga ggttttttat
     4801 ataaacttta tttgatataa agaaaaggca ttcataattg tgctatacta ctaaaaaatg
     4861 ataatacagt gaattagatt agcgctttat taaattaaga ggacagcata tcgatattgc
     4921 ttcctttttt ttgctattta tttaagggat agaagtttag aaaggactgt ttataagatg
     4981 aaaaatgata tagtgactta tgcaaacaca aaatgtgaag aaattggcat cagtaattta
     5041 gaagagtgct tttttgacac aaatgacctg tcgcaatttt taagaacggg aagaacaaat
     5101 gccagtaaac ttgccaatga cttagttgca aagaacgttc tatttaaaat caatactcgt
     5161 ccagtgttat ttgccatcaa agagcgcttt gaagagcact ttttgattcc ggtaaaagat
     5221 gtatacgaaa gtatatctga attaaaaaca gacttggagc ccaattctac agattccatt
     5281 ctacaaaaga tgattgggta tgatatgagc ttatccgatg cggtggaaca aatgaaaact
     5341 gctgtcttct atcctggaag agggcttcct atgatgatca ctggagatac tggagttgga
     5401 aaaagttttt ttgttcatac tgcttatcag tttatgaaaa aagtgaacgt tattcatgag
     5461 aatgctcctt ttaaagtact taattgtgcg caataccata ataatcctga attattgtcg
     5521 gggatactat ttggctatgt taaaggtgct tttaccggcg cagatgaaga tcgaaatgga
     5581 ttgttgcaag atgcagacgg tggagtactg tttttagacg aagtacacag attgcctcct
     5641 gaagggcaag agaaactatt tgtttatatg gatggcggaa cttattctag agtcggtgaa
     5701 actatccatc acgcctccaa tgtacgttta gtttttgcga ctacagaagg caaagaggat
     5761 tttctggaaa cctttatgcg aagaatccct atacatatcc atatcccaaa tattgaggaa
     5821 cgaggtattt tagaaaaaaa acaaattatc gaaaatactc tctttagcga aagcaaaaac
     5881 tttagcaagg ctattgatgt ttctcctaaa gtcactagtg ttgcataaat atagtcagaa
     5941 tattctgaat tattattttc ttgaaaaaag taaaaaaaat cgtatcccaa actttcatct
     6001 acatttccag aaagatcttc atgttgataa tcaattcctg aatcaatgac cgctaccttc
     6061 acatccttag aacccttggt ttcttcccaa gcggatggca tgctaatctc tcttaaatta
     6121 gcttgtttac tccaattagg atcgttatcg atttcctaaa gaatcagttg tatttggctc
     6181 ctcaacatca ttcaacggct ctagttcaac cacatagttt ggctctgcat aattgaccat
     6241 atcagaagca ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac
     6301 taataatact tgatctcgct tattataatc gctttcattt gtcctaagag tcaaatcttt
     6361 ttccaccata acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct
     6421 ttgtgtagcc tttaaactga caattagctc attgggtaca tactctattt gagagtcatc
     6481 taattggctc ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc
     6541 catgaaccca aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt
     6601 tttcatctac atctcctcct aatagtttta ttttagagtt agctctatta cttatttact
     6661 aactcaacaa cagattatca tacacttgtt aatgaaatca caatatctca ttaaaacaca
     6721 ccctaaaatc ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa
     6781 aaataaatcc cctctatttc acgcaaatca aagaagaact aagtaactac cgtttaaagg
     6841 tagttactta gttcttcttt gattattatc taactttatt attcattccc caccctttgc
     6901 gcagtgagtc ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat
     6961 catctaattc tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt
     7021 cattaaacaa atcttgttta tcttcttgcg taagattctg tttaatatac tcatctaaag
     7081 cttttctaag caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg
     7141 aagtcgcatt taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa
     7201 actttctgat tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg
     7261 aggaagtttt tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa
     7321 ttgcttgctg ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt
     7381 tcccaaaaat atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct
     7441 cgtgttcttc caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt
     7501 gttttaagag acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta
     7561 agcgcgcatt tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt
     7621 gtttatacat ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag
     7681 caacatataa atttgtatca aattctttca caaaaaagtc catatactca agatattgca
     7741 tcgcatttct catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt
     7801 acatcgcgga tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa
     7861 actcatcaaa taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat
     7921 tatattggtc tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat
     7981 agaggttatt gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt
     8041 cagtcacagt attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga
     8101 agttcatatg cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta
     8161 tcaaggttct ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac
     8221 ttttacctac gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac
     8281 ctcttttctt ttagcaatat tcatttgata ctattataac atatttattt ttagttacta
     8341 ccaaaaaaaa gatatatatg tactacctta taattaataa gtaaaaggta gtacataaat
     8401 aaggaaaagc tgaaatatat tgatttatag ctttcctcaa agtaatgacg ttatttaatt
     8461 tatcgagaag cagagaatac tcgcgacttt agtcatgtag acgaatcaga ttttcgataa
     8521 aaaagaagcc gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt
     8581 cataaaagtt ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct
     8641 cgtaagctac tctatatgtt gtgcccgcat atttgaaccc actagaccaa atacctgata
     8701 aatccccagt tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat
     8761 tcaaaaattt ttctttcaat ggcttctctt ttagcttttt taaatactta atgacatgtt
     8821 ttgaaggaac gatttcataa gccaatttct gttgccaact cctctttagt cattacttta
     8881 gaattagtaa gtgtatcttc agcaatatct ctaaaggaag aatgcatttt gttttttcta
     8941 ttcttaaatt ctacaagaat ctcatcttta tcgtagcctt ctgcaattat atcagacaaa
     9001 atatcttcgc taaaatcata aaattctttt tgtggttcaa caaattcata aagaatacca
     9061 ttctctacca actttgcttc aacttcgact ccattaggaa cattaaaatc tttaggtact
     9121 gtcaacatga ttgaattgcc ttgttttctt gtttttgtaa tcatttaaat ccctcatttc
     9181 taacagtatg taattactta gtaaatacat tataccacga gataatgtat ttacaagtaa
     9241 ttactgtgac tcgtaatgcg tccttgctga ataaatttca accacgtgtt catcttcatc
     9301 taccttataa acaacccggt gttgtctatt caatcttcta aaatacctac cttcatgtgt
     9361 aggttttaac ttctcaaatg attgggttct gttgcaaagt tttaaataaa gaataaaatc
     9421 ccttacggta tctatgattt aagct
//
LOCUS       DAFEHS010000091         9317 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFEHS010000091
VERSION     DAFEHS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.096"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..2122
                     /locus_tag="LP327_002425"
     CDS             <1..2122
                     /locus_tag="LP327_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726511.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="LP327_002426"
     CDS             2119..2739
                     /locus_tag="LP327_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726512.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            2853..2990
                     /locus_tag="LP327_002427"
     CDS             2853..2990
                     /locus_tag="LP327_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726513.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            3432..3812
                     /locus_tag="LP327_002428"
     CDS             3432..3812
                     /locus_tag="LP327_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBL1726514.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3794..4708
                     /locus_tag="LP327_002429"
     CDS             3794..4708
                     /locus_tag="LP327_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL1726515.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="LP327_002430"
     CDS             4948..5637
                     /locus_tag="LP327_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726516.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5758..5844)
                     /locus_tag="LP327_002431"
     CDS             complement(5758..5844)
                     /locus_tag="LP327_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL1726517.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6489..7226
                     /locus_tag="LP327_002432"
     CDS             6489..7226
                     /locus_tag="LP327_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL1726518.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7254..7793
                     /locus_tag="LP327_002433"
     CDS             7254..7793
                     /locus_tag="LP327_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL1726519.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            7809..7979
                     /locus_tag="LP327_002434"
     CDS             7809..7979
                     /locus_tag="LP327_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726520.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(8291..8752)
                     /locus_tag="LP327_002435"
     CDS             complement(8291..8752)
                     /locus_tag="LP327_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1726521.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            8885..9259
                     /locus_tag="LP327_002436"
     CDS             8885..9259
                     /locus_tag="LP327_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HBL1726522.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3597 a         1414 c         1454 g         2852 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     1441 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     3301 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     3841 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     5581 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     5641 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt ttcaggttga tgtcgccttt attgatttta
     5761 cttcttgttt cggttattca accaataact gaataaagag acacaaactc ctacaagaag
     5821 ggggcaaatg aacagttcaa acattagata cacctccttc cagtcgcaag actgtcggat
     5881 aggcgacaag ttattatatc ttaaaaaatt aaatttgtgt ggtgttttta agtatcactt
     5941 gttttagggt tgttagggct tgccctgacc gcctgtaagg cgcttctcga ctagattaag
     6001 ggaaattaat caagaattgt cttagaacgg cttagacaag ctttcagagt ctcacacctc
     6061 ctctttgaac aacggtttta gcaaaggagc cggaggcgac tgcgcagtat aatatgagag
     6121 catagcgatc acaccttttt agcgaccgta gggagcaagt tttccagttt agaaaaattc
     6181 gcgaaccttt ttggaggaaa aagtaggagt tttttaggcg ggaaattcaa tgtgctctta
     6241 agatatactg ttagatatac tgttagatat actgttaata ataactgtta gggattagca
     6301 accttatggg agagtaagta ttgacatagg tagtggggga taaattgtca cactatgggg
     6361 gataaattgt cacactatgg gggataaatt atcacactat gggggataaa ttgtcacact
     6421 atgggggata aattgtgttt tacatcctct tatgtctatg ataacataat ttattgaggt
     6481 gatttttatt gagtaacgat atagttcgat ataataatgg attaaattct gtccctttaa
     6541 gaaaatttac ccctatagat atggatttat tttggggaat atgttctaaa atgaaaagaa
     6601 aaggtacaaa agaagaaatt tttacattta aacaaataaa agaattaatt gattacaata
     6661 taaatgcacg tggcgaccgt tttgctaatg atttaataaa aatgtcaaaa aaaatcatta
     6721 ctttgtcttt tatttatgaa gacgaaaaaa catatgaaca attaaatctg tttcaaaaat
     6781 ttaaaatcga taaagtgaat gaaacattat taataaaggt ttctgaagaa tttgaatata
     6841 tacttaattc aatcggaact aattttactc gtttcgaatt agaaaatatg acaaacttaa
     6901 agagtagcta cgttaaagag ttatatcgcc aacttatggc tcataaagac agaagatcta
     6961 aaaaaggtca ctggtttgta aaaatagaag attttagagc tctattagca ataccagaaa
     7021 gttatcgcat gagagatatt gataaacaaa tttttgccca agcgaaaaaa gaatttttaa
     7081 acgttaattc aactcaccct gctattttta aaacttttga aatcaaaaaa attaaagcct
     7141 acaaaggaaa taagatagga tctctgcaaa tttattttga agaaaatata ccttcaatta
     7201 ccttagataa ttggttagag aaataatacc taacaattta ggaggcaatt atcatgcaac
     7261 ccaaagaaac ctttacaata aaggaattat ctgaattatt tgaaataagt aggcaagcaa
     7321 tctctaaaca tgttgataaa ttagacccgt ccttattagc taaaaatgag cgtggttata
     7381 aggttgtatt acgttctgga gtattgcaac ttgctagcaa cttaggtaac caaaggttac
     7441 tatataggtt gcatgagtat gaacaagaaa acgataaaaa cgtttatata tcaagcaaaa
     7501 acgtgattgt aaacgagttg ctagataagt tgcgtgaaga aaatgaattt ttacgagaac
     7561 aactttccct caaagagcaa aataattcta atatgcaaaa attactcgac cagcaacaac
     7621 agctcaccct acaggcaaat caacagatcg aaaaactgca agaacagtta caattagctt
     7681 atacggaaga aactccagaa gatgaatcaa ctactattct ttctgaagaa acagaatcga
     7741 tagaaaaaca cacaaaaaag aagaagcaaa aatggtggca actttggaaa taactggagg
     7801 gaacattcat gacaagaaaa aatcttctat tcggttctat tttactagga attctaggct
     7861 tcagtccgat gttattgtca gtaatcaata aagggaataa acatggtgaa ttgaaattga
     7921 atccaaatta ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat
     7981 aaaaaagact gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa
     8041 gcacgcgctt atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt
     8101 aaaaaaatga cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag
     8161 ctttagaaag ttgcttttag ctagaccaat taaaaatcac aaagtgagcg tctctatcca
     8221 ttggtagagg ggctacttgg catagtgtac attatgtcaa ctatgcaatt aaataactaa
     8281 ctacttcata ttagcttaat aaaataatca aaataactag ggggaaaaac attgtataaa
     8341 aaaatttttc attttttaaa taagaatagt aaatataaaa ataatttaag atatctatta
     8401 ctattaatcc agatagagca aaccaagtaa atattccatg tgtaaagcta aaaaaattca
     8461 tttgaatcaa ttcaaataaa cctaatatag taacaaatat attaaaaata atagctgtca
     8521 ataccagtaa attatttttt ttattttttg aattatcttc atttaaaata gaatttatgt
     8581 tagtactata aaattcacaa atttttttta gtgtctctat atctggtgtt gttttgttat
     8641 tttcccactt tgaaatagtt tgacgtgtaa cgttaaattt tcttgccaca tattcttgag
     8701 tatacccttt tttcactctt aattctctta gcttagatcc taaatcctcc attttttctc
     8761 ctatgtaaac gattatttac ataaaatttt atcataaaaa tactaacgcg ccaaaaaagt
     8821 tctttatttt ataaaacaca ctaaaataaa ataattctag taaaaaagat aggagaaata
     8881 agttatgaaa aaatttttat gtctttctgc tttaatgggt gtcttattaa ctagtggagg
     8941 aatagctagt gccacagaag ctttaaatct agatgtaccc gaagaccata ccgccattta
     9001 cggaggagga atggaagcta cagaagaagg attttcttct agtaaattga gatatgctgc
     9061 tggcggcggg gatttcaact gtggggttaa tggatttaaa gtttatgcaa attactacca
     9121 tgctagagct aaacatagtg caacagctaa aaatggacga ggcggtcaag tacgatcagt
     9181 tcagaaagct ggagtaagag cttatgcaac atgcaatgcc acacttactg gaaatactgg
     9241 ttggtggaat gtttattaaa aaagagagtt taaactctct tttttattta catttgaaag
     9301 gaaattcaaa atatgtt
//
LOCUS       DAFEHS010000092         9152 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFEHS010000092
VERSION     DAFEHS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.097"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(257..598)
                     /locus_tag="LP327_002437"
     CDS             complement(257..598)
                     /locus_tag="LP327_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL1726523.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(598..828)
                     /locus_tag="LP327_002438"
     CDS             complement(598..828)
                     /locus_tag="LP327_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL1726524.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1124..1462
                     /locus_tag="LP327_002439"
     CDS             1124..1462
                     /locus_tag="LP327_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726525.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPIQIET
                     LVQRISTREDFAIVECEIVAIFVEEGILHDPTHINVDKWQPLIYKLREYTTANQSLSL
                     NFRFQEFKES"
     gene            complement(1667..2242)
                     /locus_tag="LP327_002440"
     CDS             complement(1667..2242)
                     /locus_tag="LP327_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBL1726526.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDVSFARKIITQQMPELVMEKV"
     gene            complement(2607..3188)
                     /locus_tag="LP327_002441"
     CDS             complement(2607..3188)
                     /locus_tag="LP327_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL1726527.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASETGKGQFYYYFS
                     SKKEVCLAVIHAHMVVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWIKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3371..3997)
                     /locus_tag="LP327_002442"
     CDS             complement(3371..3997)
                     /locus_tag="LP327_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL1726528.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFSLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVIIGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4019..5347)
                     /locus_tag="LP327_002443"
     CDS             complement(4019..5347)
                     /locus_tag="LP327_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBL1726529.1"
                     /translation="MEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCI
                     NIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFL
                     SNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEK
                     LVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFHL
                     GVSVDQVADQESTAAVTFTENGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDRG
                     AIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVPY
                     SVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDL
                     ILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5506..5907)
                     /locus_tag="LP327_002444"
     CDS             complement(5506..5907)
                     /locus_tag="LP327_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL1726530.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5993..6199)
                     /locus_tag="LP327_002445"
     CDS             complement(5993..6199)
                     /locus_tag="LP327_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726531.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6294..7796)
                     /locus_tag="LP327_002446"
     CDS             complement(6294..7796)
                     /locus_tag="LP327_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBL1726532.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7814..8269)
                     /gene="argR"
                     /locus_tag="LP327_002447"
     CDS             complement(7814..8269)
                     /gene="argR"
                     /locus_tag="LP327_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL1726533.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8367..8984)
                     /locus_tag="LP327_002448"
     CDS             complement(8367..8984)
                     /locus_tag="LP327_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBL1726534.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
BASE COUNT         2511 a         1932 c         1492 g         3217 t
ORIGIN      
        1 tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa
       61 gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat
      121 gctatatatt ctttagttta agatacggaa attaatccca tcgaccacac tagtagaatg
      181 aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa aatattatag
      241 agtttactta caatgcttaa aacatatact gtataatctg atcaatttta gccatgtctt
      301 gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga gattttaatt
      361 gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa cgtgtcggta
      421 aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta cggttaaatt
      481 cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct actgagggat
      541 caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct tctgacacta
      601 taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat aatactcggc
      661 ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg ttccatcatc
      721 agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat cagaaggtaa
      781 agttaccact acagaacttc cttgtaatct agattttgta acaagcatta tcatcactcc
      841 cttaataaaa gtatactata taacactaag taacgcaaaa ttcccctttg taaactatct
      901 agaaaattgt tttgtttaaa gtatatctta ttggatataa atagaaataa attgtagcca
      961 gttcattttt tctttaaata aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa
     1021 gctacccaaa tactatagct aactccccgc taccttcaat cttgcagata aaacgctttg
     1081 gaaaaaaatc gagaaaatcg caaagacgac aggccatcct atcatgcctg attataaaca
     1141 aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca cgaaactagc
     1201 aggtgaagaa gtttcaactg ttcggattaa agaatgcccg attcaaatag aaacgttggt
     1261 tcaacgaatt tcaactagag aagactttgc tattgtagaa tgtgagatcg tagctatttt
     1321 tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat ggcaacctct
     1381 tatctataaa ctcagagaat atacaactgc caaccagtcg ttaagtttga attttagatt
     1441 ccaagaattc aaagagtcat aatgataaac atcattaggt ataaataaaa aagactcgca
     1501 gaacgagtaa aaaacaaata gaagctatac cttagaaagg tcgactgtga ctctatagat
     1561 gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga tctgtgctga
     1621 agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat accttctcca
     1681 ttactaattc aggcatctgt tgagtaatga tttttcgcgc aaaagaaaca tctgttaccc
     1741 aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg
     1801 tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc tgatggctac
     1861 gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat ttgtcttttt
     1921 cttcattcca ctcataaaag ccactcattg ggaaaacaca acgattttgc aaaaatggtt
     1981 gatgaaaggt ctttttttct tctactgtct ctgcacgggc attaatcatc aattgtcctt
     2041 ttttaaatcc ggtaaatccc cacttcatga tttccggagt gaggatgttt tcttcctctt
     2101 ttcttaacgt cacaacaaga tttgacggaa aaacttctcc agctgctaat tctgctgcct
     2161 gctccggttg tttcgtctgg gcttctcgat aataattctg aaggagctct gactctagat
     2221 caaaaaaata acgaccacac atcctaatcc ctcccaatct ttttttctat catacactgc
     2281 aacaagaagt ccgtaccaac aaaacgcttt caatttgtgt agaactaaaa aagttgtgac
     2341 aagtgacaaa ctctccagtg aatggtattc tcctgccaac tcctcggtca caagtcacaa
     2401 tattcaacaa acatgagaag ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg
     2461 gcaggcttac gacctctggg taaacggtgt tccagaagta gcttcttcga aaaataagcc
     2521 gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg
     2581 accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct
     2641 ttgatcaatt gaaaattttg tctaagtagt gatagatcct gtgtgacttt caacaataag
     2701 atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac
     2761 agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgatcca ttgattgact
     2821 aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta
     2881 cctacaggac agccatgata agtgatctgt gtctcatgat cagcaaaaat ccagtccaac
     2941 atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct
     3001 gtctgccaca cgaccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa
     3061 aagtaataat aaaattgccc tttccccgtc tcagaagctg ctagaatatc tcggattgac
     3121 gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt
     3181 gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca ataaattcat
     3241 cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac
     3301 tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc
     3361 aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa
     3421 taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg accaggagca ccaattgcat
     3481 ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac
     3541 acaagtttcc gttcattcca ccaataatca ctgtgtcgat tccatttttt ctcaattgga
     3601 gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgata atcgtatttt
     3661 catccaaata tggtttcaat tcttcgatga aatcagctcc tgaaccttct tcaactgatt
     3721 catattgact tttcctagca aacatctgtt gctcaagcat catatgttct cctgcaccgc
     3781 caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc
     3841 ctttttcttt tgcttgtttc accatttcga tcatgttttc gatcgtattg tatttttcta
     3901 agatatcttt tgtcaaacta aagccaccgc ctgctggttt caagaactcg acttgcgggt
     3961 cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt
     4021 attttaatac atcatttaaa gcttcactca ttgttggatg agaatagatt tgatctctca
     4081 ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag
     4141 aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt
     4201 tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact
     4261 tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg
     4321 ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt
     4381 tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag atataagtga
     4441 actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt
     4501 attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa
     4561 gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta
     4621 cttcttggcc attttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat
     4681 ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt
     4741 gcgcgatatc ttcatcttca cgaggaagaa attcaggatg ggcatcaaga acgacaactt
     4801 tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga
     4861 tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc
     4921 gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa
     4981 tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag
     5041 aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat
     5101 aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt
     5161 cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac
     5221 atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattcg ccttttgaag
     5281 ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt
     5341 tttccatcct ttttccctcg cttctttttt tacctaccga ttggtacaaa aatatgataa
     5401 ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac cagacaaaaa
     5461 gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa caatcgttac
     5521 ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat gcgcatcttc
     5581 tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta atactacacc
     5641 gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta ctgtggactc
     5701 tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac aagaagcata
     5761 acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg gaggagtgat
     5821 tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc catggttgat
     5881 cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc atttgctact
     5941 tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct gcttattcga
     6001 ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta ctcgtgaaca
     6061 acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct tttatatgat
     6121 tgattttaag atacatatat accagaaagt tgaccccata aaccaatact gttaaaaaaa
     6181 taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag cactcgattt
     6241 aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact ggtttattct
     6301 gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt tactaacgcc
     6361 aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg ctcatgtaag
     6421 cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata tcgaatacct
     6481 tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg cttcatttct
     6541 tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa tcggatttct
     6601 tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat accttttctt
     6661 tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc atccagaaaa
     6721 tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt atctttccca
     6781 agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat ataacgaact
     6841 tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg ggaattcgtc
     6901 acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc gatcaaatta
     6961 tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac atctgtatcg
     7021 aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact ttccatcgct
     7081 tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc caaaaattgg
     7141 gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa tccaccagca
     7201 ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc tagtgttcca
     7261 atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag gtctgctttt
     7321 tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat aaaagcaata
     7381 tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata ataataggga
     7441 agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa ttgagtggac
     7501 ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc ttgttctttc
     7561 agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc atgaccaatc
     7621 ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat ccagtttgct
     7681 agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata tccttcaact
     7741 ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc tttcatataa
     7801 aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc tgtgcttctt
     7861 cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg acaacttcag
     7921 ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt tcagtcaata
     7981 cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt gctgtacgca
     8041 aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt actattttgt
     8101 tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc gttgcttgag
     8161 tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc ccaataggtt
     8221 gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata ttagacacct
     8281 ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt attttttact
     8341 tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg caaaatcgtc
     8401 ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt gtgttcttta
     8461 gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa cgttgctggt
     8521 tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc catctttttg
     8581 aatactactt gttctttcaa gtcttccgga agtttgctag aacgctgaat ggcttcttgt
     8641 gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga aaaatgttct
     8701 tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc ttccagtgga
     8761 tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga catgcgtatt
     8821 gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg attcgggtcg
     8881 ccgatccctc tgatgctaaa gaatgatttt ttaggaacct ctagtatttg cgggcttttt
     8941 ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct tcctccgcga
     9001 aattccattt agttatttat tatcatacct ctttttgagt gacttggcta atcgtgttga
     9061 cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag ataacacgtt
     9121 tcggcgtgtc gagagagagg cggtttctat tt
//
LOCUS       DAFEHS010000093         9137 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFEHS010000093
VERSION     DAFEHS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.098"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(51..1517)
                     /locus_tag="LP327_002449"
     CDS             complement(51..1517)
                     /locus_tag="LP327_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBL1726535.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="LP327_002450"
     CDS             1904..2500
                     /locus_tag="LP327_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL1726536.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="LP327_002451"
     CDS             complement(2558..3040)
                     /locus_tag="LP327_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726537.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="LP327_002452"
     CDS             complement(3146..3343)
                     /locus_tag="LP327_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726538.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="LP327_002453"
     CDS             3638..4396
                     /locus_tag="LP327_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL1726539.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="LP327_002454"
     CDS             4456..4884
                     /locus_tag="LP327_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726540.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="LP327_002455"
     tRNA            4930..5002
                     /locus_tag="LP327_002455"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="LP327_002456"
     CDS             complement(5152..5523)
                     /locus_tag="LP327_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL1726541.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="LP327_002457"
     CDS             5738..5923
                     /locus_tag="LP327_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726542.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="LP327_002458"
     CDS             complement(6006..6194)
                     /locus_tag="LP327_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726543.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="LP327_002459"
     CDS             complement(6218..6481)
                     /locus_tag="LP327_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726544.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="LP327_002460"
     CDS             6639..6896
                     /locus_tag="LP327_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBL1726545.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="LP327_002461"
     CDS             complement(6967..7158)
                     /locus_tag="LP327_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726546.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="LP327_002462"
     CDS             complement(7299..7619)
                     /locus_tag="LP327_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726547.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="LP327_002463"
     CDS             complement(7642..8076)
                     /locus_tag="LP327_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL1726548.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8411..9049
                     /locus_tag="LP327_002464"
     CDS             8411..9049
                     /locus_tag="LP327_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL1726549.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2904 a         1820 c         1468 g         2945 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag
     8401 gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc
     8461 tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga
     8521 tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc
     8581 agaatcaaat tcaatttctg atattaacct gatctactca ggacaacaat taacaatccc
     8641 aacagaaggc tctgcacaag cagctgctga acctcaagca gccgttcaag aagcaccagt
     8701 ccaagctgaa cctgttcaag ctgaacaacc tgttgtacaa gaaacggttc aaacggaaac
     8761 acaagcagcg cctgttgctg aaacacaacc tgctcctgct gtaacagaaa ctgctgctac
     8821 tccagcaagt acaagctctg caaaagaatg gatcgcacaa aaagaatcaa gcggttctta
     8881 cacagcaacg aacggacgtt acatcggccg ttaccaatta gattcttctt acttaaatgg
     8941 tgactactct gctgcaaacc aagaaagagt agcagaacaa tatgttactt cacgttatgg
     9001 ttcttgggaa gcggcgaaag cattctggga agcaaacggt tggtactaag attcattatt
     9061 tgaacgttta aacacattca aaatatagta agaggtcatg cgatttattc tttcgcatga
     9121 cctctttttg agtttaa
//
LOCUS       DAFEHS010000094         8731 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFEHS010000094
VERSION     DAFEHS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.099"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            2..74
                     /locus_tag="LP327_002465"
     tRNA            2..74
                     /locus_tag="LP327_002465"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="LP327_002466"
     tRNA            78..150
                     /locus_tag="LP327_002466"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="LP327_002467"
     tRNA            155..225
                     /locus_tag="LP327_002467"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="LP327_002468"
     tRNA            238..311
                     /locus_tag="LP327_002468"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="LP327_002469"
     tRNA            323..394
                     /locus_tag="LP327_002469"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="LP327_002470"
     CDS             660..1853
                     /locus_tag="LP327_002470"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBL1726550.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="LP327_002471"
     CDS             1983..3455
                     /locus_tag="LP327_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL1726551.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="LP327_002472"
     CDS             3538..4326
                     /locus_tag="LP327_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBL1726552.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="LP327_002473"
     CDS             complement(4393..5061)
                     /locus_tag="LP327_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBL1726553.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="LP327_002474"
     CDS             5176..6888
                     /locus_tag="LP327_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726554.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="LP327_002475"
     CDS             6891..8663
                     /locus_tag="LP327_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726555.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2622 a         1487 c         1944 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc a
//
LOCUS       DAFEHS010000095         8603 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFEHS010000095
VERSION     DAFEHS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.100"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="LP327_002476"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="LP327_002476"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBL1726556.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="LP327_002477"
     CDS             complement(1098..2201)
                     /locus_tag="LP327_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL1726557.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="LP327_002478"
     CDS             complement(2291..3247)
                     /locus_tag="LP327_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBL1726558.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="LP327_002479"
     CDS             3528..5462
                     /locus_tag="LP327_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL1726559.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="LP327_002480"
     CDS             5919..6335
                     /locus_tag="LP327_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL1726560.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="LP327_002481"
     CDS             6349..6915
                     /locus_tag="LP327_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBL1726561.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="LP327_002482"
     CDS             6938..7924
                     /locus_tag="LP327_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL1726562.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="LP327_002483"
     CDS             complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="LP327_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL1726563.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2527 a         1658 c         1750 g         2668 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgt
//
LOCUS       DAFEHS010000096         8490 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFEHS010000096
VERSION     DAFEHS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.101"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..173)
                     /locus_tag="LP327_002484"
     CDS             complement(<1..173)
                     /locus_tag="LP327_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1726564.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="LP327_002485"
     CDS             complement(593..1714)
                     /locus_tag="LP327_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBL1726565.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="LP327_002486"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="LP327_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBL1726566.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="LP327_002487"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="LP327_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBL1726567.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSC"
     gene            complement(4304..5215)
                     /locus_tag="LP327_002488"
     CDS             complement(4304..5215)
                     /locus_tag="LP327_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL1726568.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="LP327_002489"
     CDS             complement(5232..6239)
                     /locus_tag="LP327_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL1726569.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="LP327_002490"
     CDS             complement(6236..8365)
                     /locus_tag="LP327_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL1726570.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8490)
                     /locus_tag="LP327_002491"
     CDS             complement(8370..>8490)
                     /locus_tag="LP327_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL1726571.1"
                     /translation="GQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2598 a         1728 c         1487 g         2677 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tgttgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DAFEHS010000097         8435 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFEHS010000097
VERSION     DAFEHS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.102"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(41..2650)
                     /gene="clpB"
                     /locus_tag="LP327_002492"
     CDS             complement(41..2650)
                     /gene="clpB"
                     /locus_tag="LP327_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBL1726572.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2901..3947)
                     /locus_tag="LP327_002493"
     CDS             complement(2901..3947)
                     /locus_tag="LP327_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBL1726573.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(3958..4230)
                     /locus_tag="LP327_002494"
     CDS             complement(3958..4230)
                     /locus_tag="LP327_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBL1726574.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(4265..5173)
                     /locus_tag="LP327_002495"
     CDS             complement(4265..5173)
                     /locus_tag="LP327_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBL1726575.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(5305..6537)
                     /gene="pepT"
                     /locus_tag="LP327_002496"
     CDS             complement(5305..6537)
                     /gene="pepT"
                     /locus_tag="LP327_002496"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBL1726576.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(6552..7673)
                     /locus_tag="LP327_002497"
     CDS             complement(6552..7673)
                     /locus_tag="LP327_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBL1726577.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(7670..8371)
                     /locus_tag="LP327_002498"
     CDS             complement(7670..8371)
                     /locus_tag="LP327_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBL1726578.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT         2318 a         1841 c         1452 g         2824 t
ORIGIN      
        1 gtgtcagacc ccttttttct tttatagtct caatggaaga ttagacgatt tcttctagct
       61 cttctgtttt gaaatgaagt tgtccatcaa gtaaagtaat cgttactttt gatttaggca
      121 taacatgtcc tgcaacgatt tctttagcta gtggtgtttc tacttcctta gtgataaatc
      181 gtttcaatgg tcttgcacca tatgctggtt cataagcatt ttcagcaatc cacgttttgg
      241 cttcatcact gattgtcagc aggatttctt gatgttccag acgttgtgcc aattgcgcca
      301 ccattttgtc aacgattcct ttcacgttgt ctaggctcaa tggtgtaaat aaaatcgtgt
      361 cgtcgattcg gttcaagaat tctggtttga agtttccacg taatagcgtg ttgacttgtt
      421 cagccactgc ttctggaatc gttccatcag cagtcacgcc ttccagcagt aactgtgaac
      481 cgatattgct ggtcatgatc agaaccgtat ttttgaaatc tactactcgc ccttttgaat
      541 cagtcaagcg tccgtcatct aggacttgta acaagatatt gaagacatcg ggatgcgctt
      601 tttcgatttc atccaacaag acgattgtat aaggatttcg tctgacagct tccgttaatt
      661 gaccaccttc ttcgtaacct acatagcctg gaggagctcc gactagacga gacacagcat
      721 gtttttccat atattcactc atatcgatcc gtaccatatg atcttctgag tcaaacaagt
      781 tctcagctaa cgctttcgct aattcggttt ttccgacacc agttggtcct aggaaaagga
      841 aagaacctag aggacgattt gggtcttgca atccagcccg tgaacgaatt accgcgtcac
      901 tgaccgcatc aactgcttca tcttgaccaa tcactcgttt atgaagtgtt tcattcaatt
      961 taattagttt ttctcgttca ccttctacaa gtttcgttac ggggataccc gttaatctgc
     1021 caacgacttg agcaatctca ttttcggtaa cggactcttg aaccatctta atctcgctgt
     1081 cttttgcttt agcttctaat tccttcaatt ctttttctaa ttgaggtatc gtaccatgac
     1141 gcaatacagc agcgcgttct aaatcatagt tattttctgc atcttctaat tcgtgttttg
     1201 ctttatcgat ttcagcacgt tttgctgaaa cagaattgac ttcttctttt tctgtttccc
     1261 actgcatctt catcgcattt gcttcttcac gaagttcagc taattcttct tgcaaatttt
     1321 tcaagcgttt tttacttgca tcatctgatt cttttttcag tgcagcttct tcgatttcca
     1381 actgcatcaa acgtctagta acttgatcta attccgttgg catagagttc atttctaccc
     1441 gaatcgttgc actggcttca tcgatcaaat cgattgcttt gtccggtaag aaccgatcag
     1501 taatataacg gtcagataaa gtagctgctg ctactaatgc gttgtcatgg atgttaacac
     1561 catggtggat ctcaaaacgt tcttttaatc cacgcagaat actgatcgta tcttccactg
     1621 ttggttcttt gactaatacc ttttgaaagc gacgttccaa cgctttgtct ttttccatat
     1681 attgacggta ctcatctaaa gttgtggcac cgatcaaatg taattcaccg cgagcaagca
     1741 ttggtttcaa cagatttccg gcatccatac ttccttctgt ttttcctgcc cctacgatat
     1801 tgtggatttc atcgatgaac aagaggatac gtccatcact ttttttgact tctttcaaga
     1861 ctgcttttag tctttcttca aattcaccac ggaattttgc tccagcgatc aatgcaccca
     1921 tatctaatga aaaaatcgtt ttatctttta aattttcagg cacatctttg cgtacgatac
     1981 gttgagcaag cccttcaacg attgccgttt tccctacacc gggttctcct atcagaacag
     2041 gattattttt tgtttttcgt gaaagaatac ggatgacatc acggatttct tcatcacgac
     2101 cgatgattgg atccattttt ccgcttttga cttgctgtac taaatcaacg ccgtattttt
     2161 ctaatgcttt gtattgttct tcttggtttt gtgaggtcac tctttctcct cctctcatct
     2221 cttcgatgtt tttacgcaat tctttttcag ataatccgtt tttattcaaa taaacagtta
     2281 acggataatt ttttaatttc atcaacgcta aaataacgac ttccgttgaa agaaactcgt
     2341 cgccgaagct ttcacgtatt tggtctgctt cattcaataa tcgaaagaaa ttctggctga
     2401 ggttttgtcc atattggacg tttccaccag aaacaactgg atactcatcc agtagacgat
     2461 caatttcatg ttcaaaagat tcgacatcca atccggcatc cgtatagaaa tttcgaccaa
     2521 aatgattagg ttgtaaaaag attttccata catgggcaat gtcgatatct tgatgttttc
     2581 gagtgaccgc aatctgttga gcttcagcaa tcgcttcttg aagcgtcgta gtcattttct
     2641 caatattcat acaggtatac cctcctaatt aaaggtaaga tcacttattt ctttgatctt
     2701 tacaaaaact attatatcgc tttggtcaat tttggtcaaa atatacacat ggttttttga
     2761 ctttttttct cgacttatat aaatcttaaa aaaacaaaat tgattgtatg tatataaaaa
     2821 aagagccaaa caaacgtaaa attatcttat ccataacgtt tttctttgat ttgataaaaa
     2881 gggtagatct tataaaagac ctacccttga ttttctttat tctctttttt tacttctatt
     2941 atttcttctt tgatcactct tcgccgttca gcaatcacat acacaaatcc atagagaaat
     3001 ggcaagataa aggaactgaa acgatacagt aaaagcgaag aaactgcgtc aactgttcca
     3061 acaactggtc tgaataataa caaatagaca aattcaaagg aaccaatacc agcaggcgta
     3121 ggaataaccc ctgaaagcac tactgcaaag ctgataaacg aaatggtcag tagaaaatct
     3181 attttaggat ggttttcaat cagaatcaaa taaggaagta cataccaaaa tacaagtttt
     3241 aacatattcc atagataaat ccgcaatagc gcagtcctgt cttggataat ggtttggacc
     3301 gtttccctta aagagtagat ctgctgattg catgtatcca cccatttccg caaacgctca
     3361 cttttaaata acttattcga taagctgaca aaaaatattt gaacattcaa gctgacggac
     3421 atcgccaata aaaaggcaac aatcacgatc gtcaaaagga taccagcgat gataaaccag
     3481 atcattgatg gaccattaga ataaaaaaga gagaattgga tgaccaagcc agctaatgca
     3541 taagtgatca ctgccagttt gtacataatc atatggagtg ctgtcacccc tgctccctgg
     3601 gatagcttca ttcctttttt gttataaaaa taaatctccg agatcagcgt tcctgtacca
     3661 aacgaaacga ttcggtaaaa cgcaatatag ctagaggtca agaacccatc tttcgttgta
     3721 aaattcggcg cgaaaggttc agcaatttct ttgattgagc gcccttcagc gatttggtaa
     3781 actgtcccta atataacagc cgctgccaaa acaacaacac tggtagacag caattcggca
     3841 aagatatctg caaatgattg attgattaaa taataaataa tggcaaaaat cacaatcagc
     3901 aaaagcagat tcagaatgat tttagttttc gttcctttag acgacatctc ctgctcctta
     3961 atggaaaaaa taaatgagaa tcaaatcaac aagtacacct gcagcgaatc ctatagtaaa
     4021 cttctttttc cgagttttat gatggaaaac ttttcgagag atcaatcctc ccaaaccacc
     4081 gcctaacagg ctgacaaata tcaaattatt ctcaggaaca cgccagccgc tagttctagc
     4141 ctgatgttta tcatacccca tcagacaaaa taagtaaaaa ttcacaatga aaaggtaaaa
     4201 ccacaaaaga ttgtgtaaca atacatccat tactcttctt ccaactttct gttaactact
     4261 atttctaagc tttttcttct ttctttggtg aataaactgt taatcctttt gatacaacag
     4321 ataattcaac tggaagatca tctgtcgtgt ctccatccgt tcgagtgccc acagattttt
     4381 cctgcgcttg gattttcatg cgtttggctg tgaaataagt aatgccgggg atcgtataga
     4441 atttgcctct catgattcta ggcaaataaa aaataaatcg gaaaatatct aaggaaggta
     4501 cgatcgttac atgaaatttt ccatcatcta gggtcgctga gtcgtcaaaa tttgtaaaac
     4561 cgcccacgga gttagacatc gtgactgtca acagttggga ttttcctttc caatgctcct
     4621 cgtctgtttc taaatccaaa tgatattttt tcttcttcag caataactta aaaaacttct
     4681 tgataaagat ccacgagccg taacgctgtt tttcttcttg actgatccaa actgctgtat
     4741 ctgctaagat tccgattgtc agtgtagaga taatgacttc atcattgact tgaccatagt
     4801 ctattttccg tacattgcct tccaggatca cgtcagcagc ttcttctttt ttgaggggaa
     4861 tctcaagaac acgtgcaagg ttgttgacag ttccacctgg aagtaatccg acatggtatt
     4921 gagataatgt tttttggaac gcttggacga tatgatgaat cgtgccgtct ccacctataa
     4981 ttactaaagt atcagcttct atggcttctg ctttcttgac taattccgca tcatcaatcg
     5041 atgggccagt ttcagctagt tctacatata attctggctg cttttctttt acataatctt
     5101 gaaaccagcg tgctaatttc tcaccctcat ctttccctga acttttgtta taaaaaacta
     5161 gcagcttttc cattctttac ctcctattta ctcttcaaaa aagagggcgg gacaaccaat
     5221 tccattgtcc tgccctttag ccttagacag atgacattcc atcattggga cgaacgcctt
     5281 gtcataagct gttctttttc gattttaact ctttgtgttc aattctgcaa tttttacaat
     5341 cacgtctgtt gctttttcca ttacttgtaa agaaacaaat tcgaatcgtc cgtgcatatt
     5401 ttctcctccg gcaaatagat ttggcgtcgg aatacccatg taagagattt ttgaaccatc
     5461 tgttcctcca cgaactggtt cgatcactgg agagatacca agctcgatca ttgcttgttt
     5521 cgctaattcc acgatgctca tatctttttc aatgatttct ttcatgttgt aatattgatc
     5581 atacatatct actttgattc gttcttgatc aaatcgctga ttcaactctt cttggatatg
     5641 tgtgatttgt gctttgcgtg cttcgaattt ttcacgatca tgatcgcgaa tgatataact
     5701 catagaagct tcttcaggat tcccatccaa tgccatcaaa tggaagaaac cttcgtatcc
     5761 ttccgttttt tctggaactt catcggctgg aagctgatta tgaaaatcga ttgctaactg
     5821 tagagcattg atcattgtat cttttgccgt ccctggatgt acatttttcc cttggatcgt
     5881 aatatttgct tgagcagcac taaacgtttc atactgcaac tcacctaaag gaccaccatc
     5941 gactgtataa gcaaaatcta catcgaattg ctcaacatcg aatttatccg ctccgacacc
     6001 gatttcttca tcaggaccaa aagccacttt gatggtaccg tgagggatag aaggattatt
     6061 gatcaagatc tccattgcag tcatgatttc cgcaatccct gatttgtcat ctgctcccag
     6121 taatgtactg ccatctgttg tgatcaatgt gtggccttca taatttttca aatttggaaa
     6181 atcttttgta ttcaatgtat agcgttcttc cgcatctagt acgatcgtag actcgccatc
     6241 atagttttca atgatttgtg gattgactcc tactgcatta aaatctgctg tatccatgtg
     6301 ggaaataaat ccaatggaac gcacttcatg atcaacattg cttggtaatg ttcccataac
     6361 gaatccgtta tcttcattat agaaaacatc agctaagcct aactcttcta attctttttt
     6421 caattcttgc gcaaaagcaa cttgtgtctg agtcgatgga gtcgttttgc ttgttgcatc
     6481 ggagcgtgtt tctgttttga catatcgtaa aaaacgtggc aaaagatttt cgtacatagt
     6541 attctctcct tttaattaaa ttgaaatggg tttgtatttg tttcagaaac gaaaaagtta
     6601 atatcccaat tttcttcttg tttccaagat tcaaatttct cgataaatcg ttgcttgcat
     6661 aacgattcaa tataatgacc gggatcaatt gcaatcaacc cagcgctttg catatcatga
     6721 gccgtatgat aatagatatc tcccgtgata tagacatctg ctctttgggc gattgcttgc
     6781 ggataaaatt tttcaccgct tccaccgcaa atcgcaatgc gcttcacaga ggattttgca
     6841 ttttttggct ggactattct cagtccatct agttggaacg cttcttttac ttgttctaca
     6901 aatgcctcta tagaaatttc ttgcggcaat tcacctactc gtcctaaccc aaacatttcg
     6961 actggttcat ctatagcgaa aagatcataa gcaggttcct cataaggatg agctgaccgc
     7021 atggcttgaa tcacttgttt ttcgatagtt tccggcaaaa taacctcaac ttttgcctct
     7081 tgaacttgtt ctgtcttccc tactttccca atcgctggtt gtgcaccagc ttccggagta
     7141 aatcgaccgt gtccaatcga ggtaaaactt gtaccggtat aatcaccttg tgtaccagca
     7201 ccagctgcag ctaatacttc acgcattttc tgtgcatgat ccacaggtac ataaacggcc
     7261 agttttttat aatgaatctc atgagtcttt actaaataac tctccacttc aataccgaga
     7321 agttcacaaa accagtcatt cagtccatcc caaataatat ccatgttcgt gtgtgctgca
     7381 tagacggcaa tatcatgttt caacaaatct gcatacattt tttcttgtgg ctgatcagta
     7441 accagccgtt ttacaggacg aaagattgga ggatgtttag caatcagcaa atcgattttt
     7501 ttcttgattg cttcttcgac cacttctgga cgaacgtcta atgtcatcat tactcgttgg
     7561 attggcttat ccaatgttcc gatatgcagt ccgactgggt ctccttcttc agctagccac
     7621 tgaggacaat agctttcaaa cttttggatg aaagtgcgtc cgctgattgt catggcttta
     7681 aaacctcctc aatcatttgg atttcctgct cgatttgttt ttgacgtgcc tcttgattag
     7741 ctgcttgttt gagctgttca cggatagctt ttctttgttg aagctctctt gtccattttg
     7801 atttgaagat ctcacttttc tcttcaagca gaaatggccc gaacatcagc tccatttcat
     7861 tgtaagtaac tggtttttgt tctttttcgg ctacaatgat ttcatatatt tttttatttt
     7921 cttcaagtat tttttctgat tggattttat actgattttc ttgcagccaa gatcgtaatg
     7981 tacgctctcc gatgtttggc tgaaggatca aacgttcttg tccgcttaag cgttgattca
     8041 acttgccagc ttctaggata tctcggatca gtgaacctcc catacccgca atcgtgacag
     8101 ctgtaatacc atcttgtggc tggatagcat ctagaccatt tgcaaggcgt acggtgatac
     8161 ggtcttctaa tccatctttt ttgacttgct tacgtgctga ttcataagga ccttgaacga
     8221 cttcaccagc aactgcatat tcgatttttc ctcttaatat caatgcaacg ggtaagtaag
     8281 cgtgatctga tccaatgtct gctaaacgag cggattcagg gacaaagtca cccactgcgg
     8341 ctaagcgctg agataaatct aaatggttca tagcttcact ccttctagat aattatagca
     8401 ttttccaaat aaaataagga gactaggaat aaaaa
//
LOCUS       DAFEHS010000098         8251 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFEHS010000098
VERSION     DAFEHS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.103"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..197
                     /locus_tag="LP327_002499"
     CDS             <1..197
                     /locus_tag="LP327_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726579.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="LP327_002500"
     CDS             314..811
                     /locus_tag="LP327_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL1726580.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="LP327_002501"
     CDS             898..1290
                     /locus_tag="LP327_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL1726581.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="LP327_002502"
     CDS             1274..3721
                     /locus_tag="LP327_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL1726582.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="LP327_002503"
     CDS             3724..5907
                     /locus_tag="LP327_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL1726583.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="LP327_002504"
     CDS             5904..6905
                     /locus_tag="LP327_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL1726584.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="LP327_002505"
     CDS             6920..7834
                     /locus_tag="LP327_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL1726585.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="LP327_002506"
     CDS             8079..8195
                     /locus_tag="LP327_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL1726586.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2569 a         1568 c         1754 g         2360 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DAFEHS010000099         8066 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFEHS010000099
VERSION     DAFEHS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.104"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..883
                     /locus_tag="LP327_002507"
     CDS             <1..883
                     /locus_tag="LP327_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL1726587.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="LP327_002508"
     CDS             931..3060
                     /locus_tag="LP327_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBL1726588.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="LP327_002509"
     CDS             3035..3724
                     /locus_tag="LP327_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726589.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4818)
                     /locus_tag="LP327_002510"
     CDS             complement(4132..4818)
                     /locus_tag="LP327_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL1726590.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4954..5148)
                     /locus_tag="LP327_002511"
     CDS             complement(4954..5148)
                     /locus_tag="LP327_002511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726591.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5198..5638)
                     /locus_tag="LP327_002512"
     CDS             complement(5198..5638)
                     /locus_tag="LP327_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBL1726592.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5642..6487)
                     /locus_tag="LP327_002513"
     CDS             complement(5642..6487)
                     /locus_tag="LP327_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBL1726593.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6636..8054
                     /gene="pepV"
                     /locus_tag="LP327_002514"
     CDS             6636..8054
                     /gene="pepV"
                     /locus_tag="LP327_002514"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBL1726594.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2480 a         1515 c         1713 g         2358 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaaagct tttttatttt gagcgatcac tgcctgatcg gtttgttcta gtattttttc
     4501 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
     4561 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
     4621 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     4681 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     4741 tccgggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     4801 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     4861 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     4921 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     4981 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     5041 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     5101 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     5161 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
     5221 aagcgtacgc ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc
     5281 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     5341 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag
     5401 ttgggtggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     5461 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     5521 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     5581 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     5641 ctcatgcctc gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt
     5701 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     5761 tatgaagata aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     5821 ctgtgatcat cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta
     5881 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     5941 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     6001 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     6061 agggtagcaa aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc
     6121 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     6181 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     6241 cagtatccgt ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     6301 ttttttcatc agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac
     6361 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     6421 ttcctttatg tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     6481 tcagcatcga aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     6541 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     6601 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     6661 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     6721 agcgcgatga ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     6781 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     6841 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     6901 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     6961 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     7021 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     7081 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     7141 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     7201 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     7261 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     7321 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     7381 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     7441 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     7501 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     7561 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     7621 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     7681 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     7741 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     7801 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     7861 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     7921 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     7981 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     8041 gtttggcgaa ataaaaatac taaaaa
//
LOCUS       DAFEHS010000100         7883 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFEHS010000100
VERSION     DAFEHS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.105"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(29..1369)
                     /locus_tag="LP327_002515"
     CDS             complement(29..1369)
                     /locus_tag="LP327_002515"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBL1726595.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(1606..3129)
                     /locus_tag="LP327_002516"
     CDS             complement(1606..3129)
                     /locus_tag="LP327_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBL1726596.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(3507..4118)
                     /locus_tag="LP327_002517"
     CDS             complement(3507..4118)
                     /locus_tag="LP327_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL1726597.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(4241..4366)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(4448..5164)
                     /locus_tag="LP327_002518"
     CDS             complement(4448..5164)
                     /locus_tag="LP327_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL1726598.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(5170..5739)
                     /gene="scpB"
                     /locus_tag="LP327_002519"
     CDS             complement(5170..5739)
                     /gene="scpB"
                     /locus_tag="LP327_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBL1726599.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(5723..6520)
                     /locus_tag="LP327_002520"
     CDS             complement(5723..6520)
                     /locus_tag="LP327_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBL1726600.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(6490..6861)
                     /locus_tag="LP327_002521"
     CDS             complement(6490..6861)
                     /locus_tag="LP327_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL1726601.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(6880..7767)
                     /gene="xerD"
                     /locus_tag="LP327_002522"
     CDS             complement(6880..7767)
                     /gene="xerD"
                     /locus_tag="LP327_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBL1726602.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         2244 a         1716 c         1333 g         2590 t
ORIGIN      
        1 aatatccgcc ctttttgtat gtttattttt atttagcaca taaacgatac acagcttctg
       61 cataaatttc cattgcttta tacatactac ttaattccca tcgttcattc gcctggtgca
      121 taaagtcagg agtatcaggg aacatcgcac cgaatgccac acactggttc atcgtacgag
      181 caaaagtagc gccgcctgat acaaacggtt cagtcatgtc tccagtaata tcgcgatatg
      241 tccccaatag atttttcact aactcactgt ctagtggcac gtataatgaa tcaaggaagt
      301 caaattcttc ataagtcaac ccatattttt ctgctgtttt agttaattta gctactaaat
      361 catctttctt gaatgtaaca gggattctca tatctacacc gatttttgtt tgttctggtg
      421 tgatttccaa acttgcaaag ttcatcgtta gctcgccgga ttgttcatct tctgttttgc
      481 ccacgacagc ttcaccagtt gcattttctt gaactaattt tcctaagaaa ttgattgggc
      541 caaaatcaaa tacttctgaa agagcaattg ccagacgaga aatagcattc actccttgtg
      601 ctgcatcttt cgcgtggata cttttaccaa gaacgacgat gccttcacct tgttcttcga
      661 aatcgaattc gtgttttttc aatgcttctt ttacttcaga aagcttttct ccagagtaag
      721 gagctgcatc aggaacaaca ttcaacgctc ctccagcttt tacagaaaac tcgttcgttc
      781 ctggtcctgt caagtaagct tgcaataagc ctttttctgc ataaattaaa ggaaattcag
      841 catctggcgc aaatccttgc gtaattcctt cttctttttc gttatatttt tctaaacaac
      901 gccataaatt ttcttcatct gtaccaaaga taaaacgaat acgtgtcttg aattccacgc
      961 cagcatcaat caaagctttg acggcataca ttgccgcaat agaaggacct ttgtcatctt
     1021 gggatccgcg accaactaac cagccgtctt ttatagttgg atcgaatggt tttgtttccc
     1081 aattattttc gtcccctgct ggaacaacgt ccatatgaca caagataccg aataattcat
     1141 ctccagagcc aatctctgaa taaccgtaat agccttctgg atctttaaat gttctaaatc
     1201 ctagcttttc actgatttcc aatactttat ccaaagcttc aatcactttt ttaccgaagg
     1261 gatgtccttg tcctgaatct gcttcatcta gtacggaagg aattcggatc aattcgctca
     1321 atgattctaa tgcttgttca tgatgttgtt ctgtcacaaa ttttttcatg atattatcct
     1381 cctaatatgt taaaaagggt catgtcaaat tcctccgaca caacctcttc ttctcaaata
     1441 atgacgtttt aagcctgctc catcagcaag cagaatgttc ttaacaaatg atgaaagaaa
     1501 aaacactcac tgaatggagc tattgacttg tccacgtgct tatcgatagc cgtttatcgg
     1561 tttcttcatt cttatgatag accttgtacc tgtatttgtc taggtttaga acattgctgc
     1621 tacacctagg atcacacaga tggctacaaa taatatcgca attaatttac cggtaaattt
     1681 ccaccacact gaaacatcga aacgagcaat tgctacagca cccattacga tagcagatgt
     1741 tggtgtaatt aagtttacta aaccagatgc agattgataa gcagtgatta ctaaatcctt
     1801 accgacgccg gcaaattcac ccataggtcc catgattccc attgttgcag cagctaagcc
     1861 ggaagttgaa ggaatcaaga atgacatagg gatatagaaa ataaatgtaa taatgatgaa
     1921 tacacttgaa gataatgaac ttaatccttg ttcgccccaa tgtaagatgg tgtctgtgat
     1981 taatccattg ttcataacca cttggatacc acgagcaaca gctactacga ttgctacacc
     2041 gatcaaatca gaagcaccag ctaaaaattc agagacaaat tcagactctt tcatgccata
     2101 agtgattgct actacgatgg acattactaa gaataacata gtaatttctg ggaagtacca
     2161 gtcacctaat ggaagcatgc ttttacctag aaccgtacct agtaccggaa ggtttgttaa
     2221 ccagtttgtc aatgattcaa aaatcgtgaa attcgcattc aaggttgtcc aaggaatcaa
     2281 actgatgatc atgataccaa aagtaataaa gaacaataca ttaacatgtt tttgtttttt
     2341 cgtgattact tcttgacgac cgattgcttc aatatcgaaa tgcttcatat cttctttacg
     2401 ttggttgtat actaatgatt ttgttggatc tttttcgatc tttgatgcat aacgatacac
     2461 atatacaata gaaatagcta acaaaataac gaacatcaag aatcgaagac caataccttc
     2521 acccatcgaa atcccaacag cttgtgaagc tacgcctgtt gcaaaaggat ttaccgttga
     2581 agctagacag cctacttgag accctactaa tacgattgct acagctacta ctgtatcaaa
     2641 gcctacagcc atcatgactg ggatcagtaa agcatagaat ggaatcgttt cttctgccat
     2701 tccgtaagta gagccgccta atgcaaataa cagcatcaat actggaataa gcattttttc
     2761 ttttcctttg aatttcttca caactgaagc aatcccggca tctagtgccc ctgttttgtt
     2821 gataatacct aaaaatccac caacaaccaa aatgaagaaa gagattggga ttgcagcagg
     2881 tgtttcttta gttagctccc ccgctggtac acctagcatc ccattgattg gcgccatgaa
     2941 aatatcccac agcccttgag gattttgttg tactcgttca tatgatccag cgacgatgtt
     3001 gcctgcatcg tctacactat agtgcccagc tggtataaac caagtaagta cagcgattac
     3061 tgcaataata ataaataaga ccgtatacgc tgaaggcatt tgaaattttt tcttttttgt
     3121 ttgttccatc ttcttcttcc tttcttttcc gttagagaca acaattttgg ctctaatgtt
     3181 attttatcaa aaagagtatc agaacaaaaa taataacacg cattttgtaa aacttttgtt
     3241 caaaaagttc gtttttccct attccctcct cttttttcag gtttttaaag ctttcttctt
     3301 tttcattgta gcgtttcctt ttttttataa acaaacattt tcgtctttta aaaggagaag
     3361 aatttcacat ttgcaaagaa ttgattgatt tttatgcata aatcaaaaaa atactcaaaa
     3421 caaacaaata aaacgtttac attattgcat gtcttgtatt tttttccaaa aaaaaagacc
     3481 aagaaatttc ttgatctttt tgatatttat ttgactgctg tatgacgttg ttcgaactga
     3541 tgttgttttc gacttaacca tggaagaagt tgcgcatgca gcactaaaaa gacagcactc
     3601 acgatgaatc ctttgatcaa gttgaaagga acgatcccaa tcagtacata gtttgctaaa
     3661 gacatgccca gcatctgtgt tgcgttcagc cctaaaaaca ttaagtaaag aggcgtaata
     3721 acgaaataat ttgcgatact cataaaaata gttagagcga tggttcctgt aatgacacct
     3781 aaaaatttgt ttcttccttt atgctgtttc tttacactaa agaaataata aatcgggaat
     3841 gtaaagatcg ttgttgataa gaaactagca gtgtctccta ccaaattatc cggagaaaaa
     3901 cctgtagtca gcagatgtaa tactgaacgc aagaacgctg ttgcaactcc ggccgctgga
     3961 ccgaataaga acatgctgat caataccgag atatcactaa aatcgatctt caaaaaacta
     4021 aaagccggca tgatcggaaa agcaaaatac tgtaaaacta atgcgatcgc agcaaacatc
     4081 gctactgcaa ccattttctg tacacgggtg ttcttcattt gaaatcctcc tgttaggact
     4141 cctcttcagc gaactttttg acgtttctct ccaaatgaaa aaactctatt ttccagggaa
     4201 aatagagtta gagttatttg atcaagcgtc gtcaaataag ctctatcttc tttatccaga
     4261 ctatactgtc ggtatcggaa tttcaccgat tcagctattt ttttgcaaaa taggtcgtgg
     4321 actataacca ccggtcggga atttcaccct gcccctgaag acgaacctta tataattttt
     4381 gtttacatat tcattataca tatctattca aaaaacgcaa catctaacac ttataaaaag
     4441 taagtgttca ttttgagtgg ttcagcaaac tgctgacttc ttttttgttc agttcacggt
     4501 aatccccagg acgtaggcct tgtaaagtca gatctccgta tttttcacgt tttagttttt
     4561 gcacaggaag accaactgct tgaagcatat ttttcacttg atgattacgt ccttcgtgga
     4621 tcgtcaattc cacgatactg tggtttgtct taggatccac tgataaaatt tggaatttag
     4681 ctggagatgt ttttcgtcct tcgatcttta ttccttttgt caaaggtgca agcatcgatt
     4741 ttgtagcgat cccttttacc tttgcaacgt aaactttatc cacttcatgt tttggatgtg
     4801 tcaggcgctg agcaaaatct ccatcatttg tcagtagcaa gatccccgaa gtatcgtaat
     4861 ccagtcgacc tacaggatag atacgttctt ttaccatcgg caaaagatcc gtaacaactt
     4921 ttcttccttt gtcatctgca acagcagaaa taactccttt aggtttgtac aataaatagt
     4981 aaccgtattc ttcttgataa attgggactt catccacttc cacagtgtca tgtttcccaa
     5041 cttttatccc taattccctt acaacttgtc cgtttacttt tacacgtcct gcaaggatga
     5101 attcttctgc ttttcgacga gaagtgatgc cagcgtgggc aattactttc tgcaatctct
     5161 ccattacaat tattctcctt tattcattcc atcaaagaat agatctggtg acacgtcttc
     5221 ttcaagtgaa atttccattt cttgtgtatc aggcagttct tctaatgatt tcaaaccaaa
     5281 ataatctaag aagtagccag ttgttccata taatatcggc cgacctggtc cttctactct
     5341 acctttttct tcaatcagtt tatgggcagc tagacgctgt accgctcctg aaacctgaac
     5401 tcctcgaatc tgctcgattt cagctcttgt gattggctgt ttataagcga tgattgctaa
     5461 ggtttcgacg gcggcttgag ataatgtgtt tgatgtccct tgcgcatatc gcttaagtaa
     5521 gggagccagc tcttttttag tcgttaatac ataatggctt tccgtttcaa aaatatgcaa
     5581 agctgagtgc gagttttgag tgtaagattc ttttaattct ttcactaaac ttgtgacttc
     5641 tgcttcgctg agatttaaca gtccgcttaa ttcttcatta ctgatccctt cctcgccaac
     5701 aataaaaaga agggcttcaa gttcacttaa tttcgtcatg ttcggtatct cctactgctg
     5761 gataaagcat aattgattca tagttttctt tttgttgcgc aatgatctgc ccttttttca
     5821 tcaattctag caaagccata aaagtagtga caacttcact tttagaaggt gactcaaaaa
     5881 aagaatcgaa agtacagcca tgcgcatttc tttttatacg ctcttcgata aataccattc
     5941 gttcttcgat cgttgtgtca tcgggcgtga tcgttttttc cactttttga gaaaatttct
     6001 tacgttctaa gattttctgc atggcacgga tcaaatcgat ttttttgact tgtcctcgct
     6061 tcaacggctt ttcagcatcc gctaacccat cgatgttcat aggttctttt gtgtaataaa
     6121 gacttcgttc ctctgctttt tctgtcagtc tttcagctgc atatttatat ttacggtatt
     6181 ctaaaagctg agtgaccaat tcttctcgag gatcttcttc ccataattct tcatcgtctg
     6241 tgccgaattc ttggacagga agcaatgtct tgcttttgat tgccatcaat gtagcagcca
     6301 tcactaagta ttctcctgca acagctagct cgagtgtttt cattgtatgg atataattca
     6361 tatattgctc cgtgatcgta gcgatcggta tatcatatat atcgatttcc atttgctgaa
     6421 tcagatgaag cagcagatcg agaggaccct cgaatacatc taatttaata ttgatctccg
     6481 tcaactcatt cactctttct tctttttctt tgctagccat tttccaatgt aaggctctgt
     6541 gatttcagtt gcacggatgg agacatccgg gtacttttcc tgtaattcat ctaacatttg
     6601 aaagcccata ttctcaccgc gataagacgg attcaaagaa atgtcatgga tgatcagctg
     6661 atcgttcgtt gcccagtaaa taccaataat cccttgaatg ttggttgaac cttctggcgt
     6721 aaagaaaaaa agttcataat cgtcttcaga ttcgtacgta tcaatctcct tcaacaaaga
     6781 agttttacct gtcagtttct tatggaaact caatagtccc atagcaattt tacggttttc
     6841 tttccggtaa tgtgttaaca tcttctcacc ttcctatgac taagcccttg gaaaatgctg
     6901 cttataaaca tccgtcattc ttttcttcgt gatatgtgta taaatctgag tcgtagagat
     6961 atccgcgtga cctaacaatt cctgtactgt ccgcaaatca gctccatttt ctagcagatg
     7021 tgtggcaaag ctatgccgca gcgtatgtgg tgttacttct ttgtaaatcc cagcctctcg
     7081 tactaactgc ttcagatttt tccagattcc ttgccgagaa agacctttcc catgattatt
     7141 gacaaataca tgcatttcag atgcgtcctt tacaagtaat ggacgtgctt cgtctaagta
     7201 gcgttccagc cattggatcg cataatcacc taaaggaatg atccgctctt tatctccttt
     7261 tccaactgtc tgtaccagtc caagagaaag atgaagatct cccaatttca atccaatcaa
     7321 ttcactgacg cgcatcccag tagcatacat gacttccaag attgcacgat ctcttatccc
     7381 taacgttttt gtcgtatcag gtgcttcaat cagcttttct acttctgtca gagtcaatgt
     7441 acttggtaat ttctgtgctt tcttcggcgt atcgatatgt tgcatcggat catgatcagt
     7501 gatacgctct tgccgtaaaa actgatggaa tcttctgaga cttgagatca tccgagtaac
     7561 agttgctgtc gctttttttt cttcatgcaa cttctccaga aaacggatga ctaaaaagcg
     7621 atcgacatcc tgccatgaag caattttctg ctcctgtaga aaatgcaagt actgttccaa
     7681 atcacgttca tagctttgtc gtgtattggc ggacaatccc cgttcaatat tcaagtagtg
     7741 gagataatcg ttgatttgct ctttcataat aatccccctc atgcctctct atcatatcag
     7801 ataccgcttc attctgatag tctttttcca taatctgagc aaagaaaaag ccgtctagac
     7861 actcagacgg actggtttct ttt
//
LOCUS       DAFEHS010000101         7760 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFEHS010000101
VERSION     DAFEHS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.106"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="LP327_002523"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="LP327_002523"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBL1726603.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="LP327_002524"
     CDS             complement(2032..3285)
                     /locus_tag="LP327_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL1726604.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="LP327_002525"
     CDS             complement(3291..4214)
                     /locus_tag="LP327_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL1726605.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="LP327_002526"
     CDS             complement(4435..6927)
                     /locus_tag="LP327_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL1726606.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="LP327_002527"
     CDS             complement(6934..7401)
                     /locus_tag="LP327_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBL1726607.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="LP327_002528"
     tRNA            complement(7537..7610)
                     /locus_tag="LP327_002528"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="LP327_002529"
     tRNA            complement(7668..7741)
                     /locus_tag="LP327_002529"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DAFEHS010000102         7623 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFEHS010000102
VERSION     DAFEHS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.107"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(14..706)
                     /locus_tag="LP327_002530"
     CDS             complement(14..706)
                     /locus_tag="LP327_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL1726608.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="LP327_002531"
     CDS             complement(1143..1712)
                     /locus_tag="LP327_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL1726609.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="LP327_002532"
     CDS             complement(1786..2109)
                     /locus_tag="LP327_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726610.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="LP327_002533"
     CDS             complement(2263..3075)
                     /locus_tag="LP327_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726611.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="LP327_002534"
     CDS             3429..4277
                     /locus_tag="LP327_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBL1726612.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="LP327_002535"
     CDS             4422..5363
                     /locus_tag="LP327_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBL1726613.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="LP327_002536"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="LP327_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1371 g         2469 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DAFEHS010000103         7595 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFEHS010000103
VERSION     DAFEHS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.108"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(939..1352)
                     /locus_tag="LP327_002537"
     CDS             complement(939..1352)
                     /locus_tag="LP327_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBL1726614.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1808..2293)
                     /locus_tag="LP327_002538"
     CDS             complement(1808..2293)
                     /locus_tag="LP327_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726615.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2432..2677)
                     /locus_tag="LP327_002539"
     CDS             complement(2432..2677)
                     /locus_tag="LP327_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBL1726616.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2870..3670
                     /locus_tag="LP327_002540"
     CDS             2870..3670
                     /locus_tag="LP327_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL1726617.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3689..5557
                     /locus_tag="LP327_002541"
     CDS             3689..5557
                     /locus_tag="LP327_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL1726618.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5550..6041
                     /locus_tag="LP327_002542"
     CDS             5550..6041
                     /locus_tag="LP327_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBL1726619.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6029..6583
                     /gene="srlA"
                     /locus_tag="LP327_002543"
     CDS             6029..6583
                     /gene="srlA"
                     /locus_tag="LP327_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBL1726620.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6601..>7595
                     /locus_tag="LP327_002544"
     CDS             6601..>7595
                     /locus_tag="LP327_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBL1726621.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1442 g         2233 t
ORIGIN      
        1 ttactatgaa taacataata agaaagtaaa gtggattgat ttgagatctt tttatacttc
       61 aaaaaagata acacccaact ttcaaaaata tgttttggaa gttgggacaa acgagttaat
      121 acattttaat acattatttt ttattcaaaa aaaatagcac aatggcaaag atcgtaagag
      181 agatcataag ccccaatgaa tgactaaaaa gaaaagcaga atcacatgcc atatcatccc
      241 atatttcttc aagaaaataa ataagcggga aaaagggaag gaatagtaga acatcgatcc
      301 agcaccattt ttttcttctt ttgaaataac ataaaacaat aaacatgaac gtgcagggaa
      361 gtaaaaacaa agctttactc ataactaaca aaataccagc tccttttatt tttttctccc
      421 ccacattcaa agagtaacaa tggtatgtac ttaataatag aaaaatggat caacttgtat
      481 gaataacagc tgaaaagatc tgcaccaaat aaaaaacttc cagccaaaac tgactggaag
      541 tttatttttt tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc
      601 cgatttcgta gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat
      661 ggataaaata gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata
      721 agtaaaagaa agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga
      781 caatcaaatg atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa
      841 aaacaaaata tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat
      901 ataaaaatcg cccctcattc atcgagaggc gataagttct aatttaactc aatttttttt
      961 gtttcttttg gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag
     1021 gttccagtga ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc
     1081 gaatgtcttt ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga
     1141 ttggcggaga tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag
     1201 ccaggcatat cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga
     1261 taacttgaaa cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg
     1321 aacatgtcgc gaatgctagg tacatttgcc atttttgatc atcctttcaa taagtatttt
     1381 ttatttatcc ctatttacgt aataactcta ttttgaaaaa aatgcaagta taatggttag
     1441 ctttgtgaat atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat
     1501 cattaaaata ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa
     1561 tatagaaaaa ttgaatctag aaaaccgccc gacaataaat ttcattaact cagttgaccg
     1621 aaaatgaagt agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat
     1681 aagagaaata gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata
     1741 actttctcag aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat
     1801 catctaatca aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca
     1861 ctcgataaaa accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg
     1921 tgaacaaata atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta
     1981 attcggaacg gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag
     2041 aaggtacagt gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg
     2101 attggctatg ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag
     2161 tacttttagt tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat
     2221 tctttgtttt atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa
     2281 tccctttatg catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa
     2341 acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga
     2401 agataataac ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa
     2461 tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct
     2521 tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc
     2581 ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaagt
     2641 tttcattaga attctccttt cttaaatgat aaaacaatta tatatcaaaa agagaaacat
     2701 tgcttatata acgctcagga attttagcaa tctatgcaaa aagatgaaaa ttttttttgc
     2761 tcttccatta gtacacattt tttagctgaa gctgtaaatt tcatgtctta atttttttta
     2821 gtgagctttt atactggatt tgtaagaaaa aagaaggaga ggatcacata tgtcggattg
     2881 gttggatatt gcaggaaaag tagtaattgt aactggaggt tcctcaggaa ttggaagaag
     2941 tatcgtcgaa aatttgttga agcagaatgc acaagtagcg aattttgatg taactgaatg
     3001 ccgtatacag catgaaaacc tactatcttt aaaagtcgat gtgtcttcta aaacggacat
     3061 agaagaaggg atatataaag taatgaaaca tttcaagacg atcgatggtc tagtcaataa
     3121 tgcaggaata aatattcctt ctcttctaat agatagaaat catccaaaaa gcaagtatga
     3181 attatcagaa caagtttttg ataagatgat cgctgtcaat caaaaaagtg tatatttgat
     3241 gagccaagca gttggaagaa tcttggtgca aaagggatca ggagtgatcg tgaatctttc
     3301 ttctgaatca ggattggaag gatcagaagg acagagctgt tatgctgcta caaaagcagc
     3361 gatgaacagt tttactcgtt cttgggcaaa agagttagga aagaggaatg ttcgtgtggt
     3421 aggtgttgca ccagggatac tggaagaaac cggtttgaga acacaagaat atgaagaagc
     3481 tctttcttat acgagaggta taagcgtcga acagctgaga aacggttaca gtcgaacaag
     3541 tacgatccct ctaggaagga gtggtaagct gcaagaagtg gcggatctag tctgctatta
     3601 tctttcagaa agatcaagtt atatcactgg agtcacgact aatatttcag gaggtaaaac
     3661 aagaggataa aggatagggg ggacgcacgt gacgctagta aacagatggt accaattatt
     3721 gaaattacta gtcactcaca agaaagtttc aatccaggaa gtgcaaaaag aaatcaaaac
     3781 cagtaatcaa accataaaaa aagatattgg ggagttgaat gaagaaattg ccggtattgc
     3841 aaaaatcatc caaaaaaaca aacattttga acttgaaatt tatgattttg atttttttga
     3901 tgagatcatg caaggaaaat tgaaaacagc cagtgatttc aattcggcat ccaaaagaat
     3961 cgcttattta ctaaataggc tgatcgaaac agaggatttc ttacgtatgg atgatttgtc
     4021 tgaagaagtc ggtgtcagca gaggaacagt ggtcaaggat ctaaagacat taaaagagat
     4081 gatcaatgat tttgatgtaa agattacagg cacaccaaat aaaggactaa gaataatagg
     4141 agatgagcta gaattacgac tacttttatt ttattttgtt tctccctact tttcagagac
     4201 gaaaacagaa cagtttctag aagaaaaaca agcaatcaaa cattttcaga agaagacgaa
     4261 tgctagtaaa caagaaatca cccttttaac gaaagttatc ggtatttctt tgaatagaat
     4321 ctctagtaac tatctgttga agaagcccat caatcgttac agtgggtgtt ttgaatatat
     4381 ccaagaattc aatgaactga tcaatcagtt ggaagaaatc tatgaactaa ctttgagcca
     4441 atatgaaaaa gattttatca gttttccttt gaacatcatc aacacaagta taacgatgat
     4501 aaggaaagct aactctgatt tgcgcttata tttcgacccc atgatgatgc gtattcgacg
     4561 attagatctc attgatctgg atgagtcctt cttatatcaa gagatgaaac agcacctctc
     4621 tttattgata aatcgggtaa tttttcgtta tcgattgact gatttatttt atggtgagat
     4681 agaaaaaaag tatccttttt cttaccaaat cgctagcgaa tgcatacaag cattagaagc
     4741 tatcttgcaa cataagatct ctaaagtaga gatcagctac ctaacgttgt atttggagtt
     4801 agcatttcga tatcaaaaag agcatttttc ctctaaaaaa gtagccattg ttcatacgac
     4861 aggaaaagga acagcattaa tgatccaaag acaaatcaaa aggatattgg ggagggatat
     4921 acaaataaca catgtttcag aagaacaata tgaaaaagta gatctaaaca aatatttcgc
     4981 tgtttttaca acgattccgt taaaaaatgt aaatgaagag acaccggtga tccatttgac
     5041 taatcttttt gatgactctt ggttaagggc acaatggcaa aaagcaaaaa aaaagcgatt
     5101 agccgggttt cattctactg tttttttcta tcaaatatgg aaagaaaaaa agacgtatca
     5161 agaatattta aacaaattag tcgctccttt gatcagaggc tattatgtgg atgaagaatt
     5221 tgaaaaaagg atcttgaaaa gagaatatca gcaaagcaca tttgttaaca aagaatttgc
     5281 ttttcctcac acaaccaacc agctatctca acacattgtt ttatcagtag ggctaataac
     5341 tgatcaaggt ttagcagaaa caggaggaaa tggagttatt tttttatttg ccatacctga
     5401 agaaatgacg gctgaagttg agaatgaatt atttgagcta tacgatatcc tatttgaact
     5461 cataaaaatg ggaatagatg agtttattga attagcaccg acacaagaat catttttaaa
     5521 actaatcaaa agtaaaggag agagacttca tgaatgatat gtatctattc atagttttcg
     5581 cagcttcagc ttatgtgctg caagcagtta tcagttggaa gcaactaaag catttcaatc
     5641 aaacatatag cgaactgcga agaaaaggaa aagtagcgat tggtaaacga cctgggaaaa
     5701 tccgttcagg cacgattgtt atgctagcta tagacgatca tgatcaagtt ttagaagtca
     5761 gaaaaatgca aggggttaca tctttggcaa aatttcattc attacctgtc tatgcgggtg
     5821 aagatatcca ttacatcgat cgttaccatc cctcagttcg agcggaagat cgtttgacac
     5881 aagcagcttt agaaaatgcc agagaagtat acttacgaat agaggcagga aatgaaaaaa
     5941 gtgaacaacc ttcattgcct tttattgcta tgctcaataa ttttcggata tggaaaagta
     6001 cgatcaaaga aaaaaggagt gacaaacgat ggatatcata acaaaatttg ctgagggatt
     6061 tatgaggctg ttccaaacag gtgcagaaac attcataagc tggatgacag gaatcgtacc
     6121 agttgtcttg atgctgatgg ttgcaatgaa tacgctgatt gcttttttag gtgaagaccg
     6181 agtaacgaaa gtagccaaat tatcagcgaa aaatccgctt actcgttacc ttgtgttacc
     6241 gttcttatct gcttttatgt tgggaaatcc tatgtcattt acaatggctc gtttccttcc
     6301 agaatactac aaaccaagct attatgctgc tcaagctcaa ttttgtcata cgagcaatgg
     6361 tgtattcccc catattaatc ctggagaatt gtttgtatgg atggggattg ctcaagggat
     6421 cgaaacctta gggctaaatt cgatggagct ggctatccga tacttgcttg tgggactgct
     6481 gatgaacttt attggtggtt ggataactga ttttactact ggatttgtat gcagacagca
     6541 aggaatcgtt cttagcaaaa aagtagagct ttcagttgat tagagaaagg agagttttaa
     6601 atgtatggtg atattaagat cgtaagagga aacggcgggt atggaggtcc tttgattatt
     6661 aggccgacaa aagataaaca caagttcatc tatgtcacag gtggaggtga gaggccaatg
     6721 atcgtagata aaatagaaga actgacaggt atggagtcta tcaatgggtt caaaacatca
     6781 atacctgata gtgaaatcgc gcttgcaatc gttgactgtg gagggactct tagatgtgga
     6841 atttatccta aaaaagggat accaaccatc aatatcgttc ccacaggaaa aagtggccca
     6901 ttagcacaat atatcactga agatatttat gtttcagcag tagggttaga acaaatacag
     6961 ccactaatgg aaactgatga taacaaagaa acagtaaata ctgcatctcc agataaaaag
     7021 tacaaatatg atacttctaa aaagataacc gagcaaaaag cagagcagca aaactggata
     7081 gcaaaaattg gaatgggcgc gggaaaagta gttgctacat tcaatcaatc agcaagagaa
     7141 gcagtagata cgatgatccg gacagtgatc ccttttatgg ctttcgtttc ccttttgatt
     7201 ggtgtgatac aaggttctgg gatcggtaat gtatttgcta acattatggc acctttagca
     7261 ggaaatgcag ttggattaac aatcattggc tttatttgtt cactgccatt tttatctcct
     7321 ttgcttggtc caggggcagt tattagccaa gtgattggaa cattgatcgg tgtagagatt
     7381 gggaaaggaa atattcctcc ccaattagca ttaccggcac tatttgctat caatactcaa
     7441 aatgcatgtg attttattcc agtcggtcta ggtttggaag aagcaaaagc tgagacagta
     7501 gaagtaggtg taccatcagt tctttattca agatttctta acggcatccc acgagttatc
     7561 attgcttggg tagcaagttt tggattatat agctg
//
LOCUS       DAFEHS010000104         7525 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFEHS010000104
VERSION     DAFEHS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.109"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            126..1136
                     /locus_tag="LP327_002545"
     CDS             126..1136
                     /locus_tag="LP327_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL1726622.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1205..2149)
                     /locus_tag="LP327_002546"
     CDS             complement(1205..2149)
                     /locus_tag="LP327_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL1726623.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2133..4853)
                     /locus_tag="LP327_002547"
     CDS             complement(2133..4853)
                     /locus_tag="LP327_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL1726624.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4850..6070)
                     /locus_tag="LP327_002548"
     CDS             complement(4850..6070)
                     /locus_tag="LP327_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBL1726625.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6351..7196)
                     /locus_tag="LP327_002549"
     CDS             complement(6351..7196)
                     /locus_tag="LP327_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL1726626.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         2254 a         1416 c         1185 g         2670 t
ORIGIN      
        1 agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta gacattttcc
       61 tctaggaaaa aattaactag caccacgggt aaaaataata actaattaca ggagtgcaaa
      121 ttttaatgaa aaaaaaatca attattttgg cagctactag tgcgttggct gttttaactc
      181 tagcagcttg ttcaggcgat actaataaag atatcgcaac gatgaaaggc ggaacgatca
      241 ctgtatctga tttctatgat gaagcaaaac ttgaatcatc gaaccaatcc ttagtacaac
      301 ggatgatcat ttataaagta ttcaataaca aatatggcga taaagtaacc gataagcaag
      361 tggatgcaga atacgacaaa caagcgaaat cattaggtga tacttttgaa tcacaattag
      421 aagctgctgg atatacgaaa gatacttata aagaatatat tcgtaacaat ttagcttttg
      481 aagctggctt gaaagctcac gtagatatta cagatgacga tttgaaaact gcttggaaat
      541 ctttccatcc agaagtagaa gcacaaatca tcaaattatc aagtgaagat gaagcaaaag
      601 atgtgaaaaa atcagcggat gatggagatg atttctctaa attagccaaa gacaaatcaa
      661 ctgatacaga gacaaaagaa gatggcggta aagttaaatt cgactcaacg acaacaacta
      721 ttcctgcaga agtaaaagaa gcagctttca aattgaaaga cggtgagatc tccgatgtta
      781 ttactacaac aaatcctact tcttatgcga cagaatacta cgttgtcaaa atggtgaaaa
      841 accaaaataa aggcaatgac atggataaat acaaagatca actaaaagat atcgctactg
      901 aaactaaact aagtgacaat gcattcacaa ctaaagtaat cggtgaagaa ttaaaagatg
      961 caaatgtaaa aatcaaagat gatgcctttg aaaatgtgtt gagtgcattc acaacaactt
     1021 catcaagtac gaaagactca agtgaaacta cagcttcaac aaagagttct gacacaaaat
     1081 caactgattc aacaaaagaa tcttcaactg aagaaacaac tgattcatca aaataaaaga
     1141 aaacaaaaaa agcgaagact tatgtctcgc tttttttgtt ttgaatgaaa aaaataaatt
     1201 tttgctataa atcttttggt acatagaaac ttcggttgtc cataccgaaa atccgatcgg
     1261 tgtattttcc tggttctgtt tgacgaatcg aagtcaacat catctgcatc gaagcatcga
     1321 tattgtcgat ttgatgtaat atttctgctt ccattattct tggacgtaca ggtgagccat
     1381 attctaataa gccatggtgt gctaaaacca tatgacgtaa tacgagaaca tcttcgtcag
     1441 cttcgctgat tttcaacgca agacaggctt tagttatttc ttcgtcaatc aataccagat
     1501 gaccgatcaa atttcctgca agcgtatatt ctgtcgacat aggaccggaa agttcaatca
     1561 cctttcctaa atcatgcaaa atgataccag catacaacaa tgctcgattc agctcaggat
     1621 attcatcggc gattgatttc cctaagtgaa gcattgtcaa agtatgataa gctaatccac
     1681 tggcaaatgc atgatggttt ctttttgctg ccggaaactc aaaaaattgt ttttgatatt
     1741 tattaagtag atagcgaaca atacgattcc aatgcgcatt ggtgatctca aacaacaatt
     1801 gattgatttc ttcttccatt tcttctttct tcaaaggggc tctttccatg tataaactag
     1861 gctctcctgg ctcatctacc cttgttaagc gcatatgaag aatctttact tgagggtttc
     1921 cttgataaac ctctctttta ccgtttagaa aaacgactgt gccggctgta aatttattga
     1981 tttcctcctc cgaagcatcc cagtatttac catcgatagt acctgaagta tcttggaaag
     2041 taaaagcaat aaattttttt ccgtttttag cgattcttac atctgcactt ttgatcagga
     2101 caaattgttc aaactgctca tcgattacta aatcacgaat ttttttcatt atttgctcct
     2161 tctaattgtt ttacgatttg gttcatctca tggtaatagc ttaccatttc tttatcagaa
     2221 gaaaaacaaa ttatttgata cttttctcca aatttagcta aaagttcagc taattgatac
     2281 tttcgctgat gatcataatg aagccagcca tcatcaatga tgatcgggca tatcgaacgg
     2341 ttagcttcta atgagaggta agcaaatctc atcgccataa tcagctgatc tttcgtacct
     2401 gtagataact catagatggg aaattctgta tatccgtctg ttaaagtcag tgtatcattt
     2461 ttcagtaata tcttctgata atttcctttt gttaaaatac ctaaataatc acctgctgct
     2521 cttaacaact ggggtaattg tctttcactc atctctgttg aaagatcacc taagaatgtc
     2581 ccggcgattt caagtgctcc ccattcctcc agtaattcct gtaattttgc tttttgccgg
     2641 ctagcttgtt gataaagttc gtccaatgtg ccgtctcttt gcaactcttc tacttgaagt
     2701 tggagtcgtt gccgattttc acttgcttct ttttcttctt gctggagttg ggattgcttt
     2761 tctttcagtt ggcgcagtct tacagataat ttctcttttg tcagctcttc aggaaatagt
     2821 ggagtaagca tttccgataa ttcattgtat cttttcatct gctgttcggc ttgtttggct
     2881 ttttgtaaaa agacaggtac ttccgaagga taagataagc ctgcttgagt caattgttcg
     2941 ctgtattctt gtatccatac tttttgtttc tttttcaatt gattgatttc ttgcttcaat
     3001 aaagtatttt gctgatagct tctggcaaat ttcatctgtt ccatctcttg tataaaacgg
     3061 gaaagcaata ccattttttc tgaaattgtc ttatcttgta tcggtaacca ttctttaaca
     3121 aactcaaatt tttggtcgat ttgtcgtagt ttttcttgta tttcctgttc tcttttatgt
     3181 ttccgctcaa atacgccata taatttctga taatctatga cttcctgtcc atattctttc
     3241 atgagtttga gcgtatccat ctttcctaag tgccgatgat ccgcaaaacg atatacttct
     3301 tgctgaagaa tctgtttttc ttctccagtt ctcttttgtt tttcttctgc ttcagctaat
     3361 tgagctaagg ctagatcaag ttgtcctagt ttttcttgcc acattccttt tatctcagag
     3421 aaatcatttt tagttttaat ttttggttgc cttttgtaga agaaatagcc aatcatgcct
     3481 gcaccaatta gtaaaaggat cactttcaag ggcattggta caaatatacc tgcaatgatt
     3541 attgctccgc cagccatcag catatacggt ttagatgaag agactggtgc tatctctgac
     3601 tgattttgta ataatgctgg ataggtctct tccaagtcat tcacttctgc ttcaatcatc
     3661 tcttgctgtt ctttagctat ttggagacgc acagaaatct ggctttctag atcagcaatc
     3721 cttctttctt cagccagaag atgcttccat tcttcttcat ataaaaattc ttccggtaac
     3781 ggatttttct gccaatgcca cctttgttcc aaaatcttca attctgaatt tgtttctcta
     3841 gtctcaattt gtaatctttg ccattcttcg tttaattgtt ccgctgtaaa ttgaagttgc
     3901 tggagatctt ttagttcctg ctcctgctct aaataaaaat aatatctggc tgactgctga
     3961 tccatcccgc tatgcttttc cagtgttgct tctaattctt ccaaacgctc atttgtttgc
     4021 tgataccgtt tggagaaaga ctccagatct aaaagaagtt gctcatcagg aagacctgta
     4081 gtgctgtttt ttttgagaga ttgccattct tcatataaag aaaaattaag ttcttgctgt
     4141 ctggctaatg aaaattgttt gttcgcctct tttaattgtg ccaataactg ctgctgttgc
     4201 gactcgtatt cctgttcttg ttggatcaac ttgccaaaat aagcttcctg ttcttgtttt
     4261 tgatgaattt tttcttgcag ctctttccat tcatttaatt tttgattgat tagtaatttt
     4321 tggccttttt tcttgaataa ttgttgtgct ttctgataat attcttgccg cttttggaat
     4381 acttgagaac tgcccgtgat gcctaaagaa atcagcgcat catgtagctc tttttctctc
     4441 aaatgatcta gttccatcaa ctgctcttgc tggaaagtaa atacctgctg gaataactct
     4501 ttcgtcaaag gtgcgatcag tttttctagt aactcatcac ttcccacttg atcccccagc
     4561 cacaccttcg attttccgcg attttgctgc ttgtagcgct cgacagcaaa ttcaccatat
     4621 acaggatgtt taagccatat tttgccgcca taggctgagc cgtcttttgg tgtataatcc
     4681 ttttttttac ggccttttga agggaaacca aataatattg cttgaataaa ttgataaatc
     4741 gttgattttc ctgcttcgtt tcccccatag atcagctgat tgcctgaata aaagtcaaaa
     4801 cttttttgac gccattttcc aaaagcagca atctcgatcc ttaaaatctt cattcctgat
     4861 cctcctcaaa taaaaaatct tgattaagca gctgcttggc tttttcaagt gtttcttctt
     4921 gatattccgg cagttcgctc aatattctag ccgcttcttg atgactatat acttcattta
     4981 aaatttgttg gaaatcttga ggtgtctgat agtattgaaa taactgttcc atcaatttag
     5041 cagaagcagc aagtggggtc ttcgtataaa aagtattctc tatgagagaa atacgccaaa
     5101 taaagaaatc atttatccta tctgagaatt cctctaacaa atagtctaat agctctcctg
     5161 attgtatccg ttccaaaaca tctgtattga gatggtcata atcttttaat tttatctcaa
     5221 ttaaactaaa tccatcctct acttttgaaa gctgctgacg aatgtgaaca atgacctctt
     5281 ttgtcgttct tgctttcctc aatgatattt cctttgtctt ccaatagacc tctgctactt
     5341 taatcggatg tatttggcaa cttcctttac ttaattcaac tagtagaata gatgtagaag
     5401 cttgttcctt tttagtatga ccttgaggcg cacctggata aacaattgtt tgcttttcat
     5461 ttaagaccgt tggcacatgg atatgcccta aagcccaata atcgtatccc ttttcttgca
     5521 tctttgaggg ttggaaagga gcatagtttc ctttttgaga gacgccaggt tccccgtggt
     5581 acaacccaat atggtaatct gtgagttctc tagaaggaaa ttccatcact ttgtcttttt
     5641 gaatccacgg ttgacgataa ctaaatccac tgagagagac agtttctcca ctttttatcg
     5701 ttattttttt tgtctctacc gtttcggatg taaataaatg gacattttta ggaaaggaga
     5761 accaataccg ctctttttga tagaaatcat gatttccgaa tattataaat acaggtatat
     5821 ggttcttttc taaccgtttc atctgttctg aaaaatgttt ctgtattttt aatgaaggac
     5881 gattttgatg aaaattatct cctgcaagta aaacaaaatc aacgttattt attattgcct
     5941 gttcaatgat atttgataaa acggtcagat tcgtcttcaa tagtttttcc tgtacagttt
     6001 tatcaagagt tgtcagtcct tcaaatgacc gatccatatg taaatctgcc gcatggataa
     6061 atttcaccat taaaaatttc ctcctttttt tcttttatca taccattttc aatgtttttt
     6121 ttcatcaata accgaaaatt tgttcgtatt atgcaaagaa attacgataa atggccgact
     6181 tcaaaaaata agtttattcg aaaaaaagat tgtgacatga tttttgtcac aatcctaaat
     6241 atctatgtta tattcgcttc gtttgacact gaagaagtac tatttgtact ggaatttatg
     6301 ctccattttt ccgtatttag aattctatgt tctatactac cttaattagt ttatatggta
     6361 agaattcttt caaatgcttg agatactccg tctttatcat ttgaaacagt gacataatca
     6421 gcttttttca tgatttcttg atgactattt cccattgcaa aactaatatc tgcactttca
     6481 aacatagata agtcatttaa actatctccg atcgcgattc tcaaaggttt ctttaaattg
     6541 tagattgatt ccaaacgatc aaacaccaca cctttagata cattttgagg tacgatttca
     6601 atatttacag gtgccgattc agtaacagac acatttagca tctttttcaa atggttaaat
     6661 atattagctt tcaattgacg attaggaata aaaataaaaa atttgtaaat gctgatagca
     6721 tccttttcta taatttcctc aatattatta taagggattg aacaaataga tttttcatac
     6781 tcaagctcat agtttagctc tttgatagaa aattcactcc ctacctcgtg aaaaattttt
     6841 ttcattttat ttttataatt ctgagattca taacttccga gatttgtata tattttggtt
     6901 ggaaatttcg ataggacaga atcaattttt tttacatcat ttttatcgaa ggaagactca
     6961 aatatctttt tttctccatc aaatccaaca gaaccattta accctactgc tggtatcctt
     7021 aaaccagatt ttttgagaat attattaatg tcttctactt ctcttccaga acaaatgaat
     7081 ggaaaacagt cttccggtaa atgtttcatt gttcttgcat ttttttgact aatgctctgg
     7141 tcagaattca aaagagtacc gtctaaatct ataccaatta gtttcatttc atccatgtct
     7201 cctatacgtt gattataaat tcactgctta ttgtaagtct aaatttctca caaaacatat
     7261 actaattata cctaatttaa gtaagtacaa acatcatttt aagaatagga aaggacattt
     7321 atatactatt ctctgtattt ttgatttata aataaatatg aattgaatcc aaatcagcta
     7381 tattactatt ttataataac caactataaa aaacgaagtt aggtgaaaaa ctaacttcga
     7441 tattcataaa agactctatt agttcaactt agcaaaagat ttaaacaaaa aaagatgaac
     7501 tatttctagt tcatcttttt gcctt
//
LOCUS       DAFEHS010000105         7087 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFEHS010000105
VERSION     DAFEHS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.110"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(82..354)
                     /locus_tag="LP327_002550"
                     /pseudo
     CDS             complement(82..354)
                     /locus_tag="LP327_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            536..871
                     /locus_tag="LP327_002551"
                     /pseudo
     CDS             536..871
                     /locus_tag="LP327_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            1036..1263
                     /locus_tag="LP327_002552"
                     /pseudo
     CDS             1036..1263
                     /locus_tag="LP327_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1389..2360
                     /locus_tag="LP327_002553"
     CDS             1389..2360
                     /locus_tag="LP327_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1726627.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2501..3814
                     /locus_tag="LP327_002554"
     CDS             2501..3814
                     /locus_tag="LP327_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL1726628.1"
                     /translation="MKRRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNDKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIISLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3857..4723
                     /locus_tag="LP327_002555"
     CDS             3857..4723
                     /locus_tag="LP327_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL1726629.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4720..5568
                     /locus_tag="LP327_002556"
     CDS             4720..5568
                     /locus_tag="LP327_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL1726630.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5582..7054
                     /locus_tag="LP327_002557"
     CDS             5582..7054
                     /locus_tag="LP327_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBL1726631.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWINDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMTAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPHHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
BASE COUNT         2217 a         1197 c         1467 g         2206 t
ORIGIN      
        1 tccatgttcc ccactcctca agtgttttct ttagtatacg cctataggag gaaaaacgaa
       61 tacaatttaa ctcaatttat atagtatttt taatgctact tttttctgtt tcataaaggt
      121 aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac
      181 acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcact ggatatcttt
      241 accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca ttgccaattg
      301 tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg ccataaaaac
      361 tccttttaaa aaatgatata atttacatgt aaaatttact tgtagcttta ctgttatcat
      421 agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt tttgaatgaa
      481 tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta gctcggaaca
      541 gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg gtagaaaagg
      601 caaactttat caaacttatt acgacggtat ggagaaacag ctcaacgcat taggattagt
      661 aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg aacaaattaa
      721 aacggaaggg ttcgactgtt ccgaagaagt gatacgcaag caatctccat taattagtga
      781 tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat cgaatggaat
      841 actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt cctaccgtgc
      901 attttcgcac ggatgttcca gatactattt ttgaatgtat aaaactgaaa acacaagaat
      961 cctattaaat caacgattct tgtgttttta atttttcact accgtgcatt ttcgtttcta
     1021 tgttccatgt accccaatct tgaaaaatta attaaagact tatataaagc cattgatgac
     1081 ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat tggtcctgtt
     1141 taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga tgaaataaaa
     1201 ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc tgaatacgaa
     1261 aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat attcctgcta
     1321 atttggaatc gtttccttct tttggtataa taatacctga aattcttttg agaggagttg
     1381 ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt ggtgtgggta
     1441 cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa aaagtaatgg
     1501 ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg aaaacgaata
     1561 gcagcaatac aatagctctt attttaccta cgatttggca tcccttcttt tccgagtttg
     1621 cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg tgtaatgcga
     1681 acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt aaagtagacg
     1741 gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat ttaccatttg
     1801 tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat aattatttag
     1861 gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg tgctatgttg
     1921 ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt tacaatattt
     1981 gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt agcaatttgg
     2041 aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt tttaccatca
     2101 atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag gaagcaccta
     2161 aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa gactatttac
     2221 tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa acactcatgg
     2281 cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc tttcacgctg
     2341 gagaaacgac taaagagtaa aaaaccggtt tataacgatt ttttttgaga taggttgaca
     2401 gaaagtggtt tcaatgatat cttaaaacca attaattgga aacgttacca attaattgga
     2461 aaataaaacg gaaacgtttc aaaaaatagg aggaacatcg atgaaaagga gaatgttagc
     2521 aattgcagca attttagcat taacagctgg aggacttgca ggatgtggca gtaaggagaa
     2581 aagttcaggc agcagtgatt caggagaaat taagatttgg gttcagtttt ctgatgaaac
     2641 gcctgaagga aaagcttggg cagagattgt gaacaatttt aatgatgaat acgagggtga
     2701 atataaagct gttactgagt atattcctcg tagcggaagc ggcggtggat atgaggataa
     2761 agtaaatgct gcaattacaa ccaataccct tcctgatgtt attacgttag atggaccgaa
     2821 tactgcagct tatgctcatt caggagtcat tgctccatta gatgattatt tgaaagatgc
     2881 ggacatggat gatgtattag acagcattat tcagcaagga acctacgatg gcaaaatgta
     2941 tgcgtttggt ttctcagagt ccagtgtggg tgtctattac aataagtcgt tgttcaaaga
     3001 tgctggaatt acagaagatc aattgccgac gttagaagat ccttggactt ggacggaatt
     3061 tcaagcaatt tgccaaaagc tgactgataa atacaaaacg cctgcaattg atatgcaaat
     3121 ggcgtcggga gacgaaatgc tgacctacgc atatacacca tttgtatggt cgaatggcgg
     3181 cgatgtcgtt aatgctgatg gaaccaaagc cgaagactat tttaacgata aaaaatccgt
     3241 tgaagcgttg gaatttattc aagaattggt tgaaaaaggt tacaccacaa tcagtcctat
     3301 tgaaaaagga tttgaaactg gcaaatatgc catgctgctg agcggttctt ggacaatggc
     3361 agatttagag gaaaactatc aagagattga ttatggtatt ttgccttacc ccgtttcaga
     3421 tgcaactggg gagttagttt caccaaccgg cagctggcag ttagcaatga ctgagaaaac
     3481 agagaaaaag gatgcttctg cggcctttat taagtttgca accagtacag aatctagtcg
     3541 tattattagt ttagggaaca gtgttctacc tatcagaaat tcaacagttg atttaattaa
     3601 agatgaagtg tcggaaggaa tgcaggtatt aattaagcaa aaccaaattt ctggacatgc
     3661 ccgcccagtg gttgtagctt atccgcaagt atctagagca ttccaacaag cagttcaaga
     3721 tattagctac ttcgaggaaa acccagatgt acaaaaagta gcagatagtc gcgctgctga
     3781 aatgcaacag gcaattgata cttcactaaa atagaagttt ctaaaggggg ccaatcaagt
     3841 gttaaaaaaa ccgacaatga aagacaattt gataggttac ggctttctag cgccagcttt
     3901 gctgctgctg acgatttttc tgatcatacc cgtaatcatg gtaatttatt atgcctttac
     3961 tgattattat ttgttaacgc cagatgctag acaatttatc ggcttagaaa attttaagcg
     4021 actgttcgct gatccaattt ttgtaaaaag tatttggaac acggctaaat ttgttgtttg
     4081 gattattccg gtccaattag gggcagcttt aggaatggca cttattatca acaaaaatcg
     4141 taaaggaaat atctttttta aagtagcctt ttttgcgcca gtcgtgatgt ccttagtcgt
     4201 tatttcgatt ttatggttgt acttgttaaa tcctaatgaa ggtctggtta atacgctatt
     4261 tgcaaagatt ggaattgctt cgcagccttt tttgactagt cccaaacaag caatgtatac
     4321 gcttgttttc gtatcggcat ggcaaggtgc aggatatcag atgctgctgt ttttaggggg
     4381 aatgcaaaat attccgcaag acgtttatga ggcagccgaa attgatggtt tttctaagtt
     4441 tcagcaattt cgctatatca ccatgccatt attaaagccc acggcgattt ttattttact
     4501 taccacattg atttcggcct ttaaactaat cattcagcca atggtaatga ctcaaggtgg
     4561 accaatgaat tctactatga caatggttta ttacatttac caaactggat ttacagaccg
     4621 tatggtaggc tactccagct ccattgcgtt aatctttacc accatgattg gtttaattac
     4681 aattgctcaa agaaaattgg taaaggagga tgatccagca tgaccaagaa atttggaccg
     4741 ctaatgatcg tagaatattg tttgctgttc cttttagcgg cattattcat tttcccaatg
     4801 ctgtggatgg tagtttcctc tatgaagcct gaagcagatg tctatcaaaa tttagcttcc
     4861 ttcaaagctt ttttacctag tttgaatcca gctaattggt ttaaaactta tcaagaagtt
     4921 atttcccggt tcagtgtggg aacttattta ttaaacagta ttttttatgg tttaaccttt
     4981 gcctgtgggt caatcattgt aaactctttg gcgggttttg cctttgcgaa ggttaatttt
     5041 tcaggcaaga aaattatctt tggagctttg ttagctctgc tgattgtccc gatggagacg
     5101 gttttaattc cgcaatttac catcgttaat tcactaggct tagtgaatac tcgcttggca
     5161 gtgattttac cagcgatggc cagcgcgttt aatatctatt tgtttagaaa tttctttatc
     5221 gcgattccag aagaaatcat tgaatcagcg aaattagatg gtgcaaatat tgttaaaatt
     5281 ttctttcaca tcatgctgcc aatgtctaaa ccagccatag ccacagtagg tgtcttatcc
     5341 tttatctcca gctggaatga ttatatttgg ccgttaatgg tcttaactga cagcagcaaa
     5401 ttttcaatgc aagtggcaat tactacgatc aatactacac agccagttta tatcaatcaa
     5461 gtgatggctg ttttaaccat tgcaacgatt ccactgatta ttgtttatat cgttgcacaa
     5521 aaatatattc tgcagggact tggcggttct ggaacgggta taaaatagga gtgaatagtg
     5581 aatgttgcaa aaacttttaa aggccaatga gtttattgag aaaaatgctc aaacagtcaa
     5641 tcaagaatac cggaataaat ttcatttgat ggctccgatt ggctggatca atgatccaaa
     5701 tggttttgtt tattatcaag aggagtatca cttgttttat caatattatc cttacgacag
     5761 catctgggga ccaatgcatt ggggccatgc taaaagcaag gatctaattc actgggagca
     5821 tttgccagta gcactggcac caggtgaaag ctatgacaag gaaggctgtt tttccggcag
     5881 tgccatagaa aaggatggca agctgtattt gatttataca ggacacaatg ttgtggaagg
     5941 gcaagttcga caagttcagt gtttggcagt ttcagaagat ggcattcact ttgaaaaata
     6001 tgccggaaat ccaatcatcg cagaagatca cttagcttct gttgcaacga ccgaagattt
     6061 ccgcgatccc aaagtgtttc agcgaaaaga agactatttt actgtagtcg ctactaaaac
     6121 tgcagatgct cgtggacaaa ttgtgatgtt ccattccaaa gatttactaa cttgggagtt
     6181 ttattccgta ttgctggagg gaactccaga acaaggcatt gtatgggaat gcccagactt
     6241 gtttgaacta gatggcaaag aagtcttgat tctttctcct attcaaatga ctccacaagg
     6301 caatgcttac caaaatacga actcaactgt tgctttcatt ggtactgtcg attggcaaac
     6361 aggcaaattt ctagtagaga attttcatga aatcgatggc ggtttagatt tttatgcccc
     6421 ccagacctgt gaaaacgaag caggccaaag aataatgact gcttggatgc aaatgtggca
     6481 gcgtaaaata cctacgaatg atctgggcca tggttggtca ggttccatga ccttgcctag
     6541 agagctgagg gtggctgata atcaattgtt gcaacggcca gtttcatcaa tttatcaagt
     6601 aattgatccg caggaaagga ttgaatgggc gaagactgaa gagaatcgat tcttactgaa
     6661 ggacttagta tatgaccagc actatttacg cttggaaatc aatttaaaac aggcaacgaa
     6721 actagaactt cactatgcga agggacccca ccatacatta ctgcttaact atgatgttga
     6781 acgggagtgc tttactgctt ccagggaaca catgccgtat gatttatcag gagctgaaaa
     6841 ggaagtgttg aaaaagcgca gtgtgacgat tcctttggaa aataattgtc taaccatcga
     6901 aattttccgt gatacttcag ctattgagct ttttctaaat gatcgtctga ctatgagtac
     6961 aactttttat gagacagaaa aaggacagga ttttcttttg ctggcagaca gtgaagtaac
     7021 cttttcattg gaaagcggct taattaatct atagaaggga gagctcagtg atgatttatg
     7081 gttcaat
//
LOCUS       DAFEHS010000106         6828 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFEHS010000106
VERSION     DAFEHS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.111"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(99..1229)
                     /locus_tag="LP327_002558"
     CDS             complement(99..1229)
                     /locus_tag="LP327_002558"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBL1726632.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="LP327_002559"
     CDS             complement(1878..3080)
                     /locus_tag="LP327_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL1726633.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="LP327_002560"
     CDS             complement(3140..3343)
                     /locus_tag="LP327_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBL1726634.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="LP327_002561"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="LP327_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="LP327_002562"
     CDS             4880..5692
                     /locus_tag="LP327_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726635.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         2101 a         1367 c         1053 g         2307 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaata
//
LOCUS       DAFEHS010000107         6643 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFEHS010000107
VERSION     DAFEHS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.112"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(1..859)
                     /locus_tag="LP327_002563"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="LP327_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="LP327_002564"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="LP327_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL1726636.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="LP327_002565"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="LP327_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="LP327_002566"
     CDS             complement(2603..3421)
                     /locus_tag="LP327_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL1726637.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="LP327_002567"
     CDS             3582..4271
                     /locus_tag="LP327_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL1726638.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="LP327_002568"
     CDS             4285..5787
                     /locus_tag="LP327_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL1726639.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="LP327_002569"
     CDS             5800..6276
                     /locus_tag="LP327_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL1726640.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="LP327_002570"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="LP327_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAFEHS010000108         6543 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFEHS010000108
VERSION     DAFEHS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.113"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            126..863
                     /locus_tag="LP327_002571"
     CDS             126..863
                     /locus_tag="LP327_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBL1726641.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(1028..1249)
                     /locus_tag="LP327_002572"
     CDS             complement(1028..1249)
                     /locus_tag="LP327_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBL1726642.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            1280..2311
                     /locus_tag="LP327_002573"
     CDS             1280..2311
                     /locus_tag="LP327_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726643.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            2298..3701
                     /locus_tag="LP327_002574"
     CDS             2298..3701
                     /locus_tag="LP327_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBL1726644.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            3767..4402
                     /locus_tag="LP327_002575"
     CDS             3767..4402
                     /locus_tag="LP327_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL1726645.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            4451..5131
                     /locus_tag="LP327_002576"
     CDS             4451..5131
                     /locus_tag="LP327_002576"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBL1726646.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            5266..6495
                     /gene="rpsA"
                     /locus_tag="LP327_002577"
     CDS             5266..6495
                     /gene="rpsA"
                     /locus_tag="LP327_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBL1726647.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT         2227 a         1117 c         1405 g         1794 t
ORIGIN      
        1 tcaaatggat attccgtctt tttttatttg atgctggaaa gtttgcattt ctctcttatt
       61 tttttaaggt ataatcatac ttgattaaga aaaaggggtc actcagatag aaaaggtggg
      121 agaaagtgaa aatatttaga cgaatactaa ttggcttagt catcatttta ggattactcg
      181 gtggagccgg ttattactat atacagcaaa atacgtacga accatcgtca gaagcgcaag
      241 tgttggtaaa agaaggaaaa aacaaagaag attacttatt ttttgaagga gagaaaaaga
      301 atcatggaaa gtctccatta cttatttttt atcctggtgc tctcgtagag ccagaaagtt
      361 atagtatttg ggcgcaagat ttagcaaaaa acggcttttc agtcgctatt gtcaaaatgc
      421 cacttgatct agcagtgatg ggcggaaaca aagcggataa ggtcaaagaa gattttccaa
      481 attccgatta tgtgatcggt ggccattcat taggtggcgt aatggcaagt cgttatgtca
      541 ataagaatca ttcagacaaa catttaaaag gggtattctt catggcaagt tatcccgaca
      601 aaaaaggaac attgacagaa tcagctgttc ctgttttatc gatcacggcg acaaaagata
      661 aagtattgaa tcaggaagct tatcaagaag ctaagcagta tttaccggaa agaacagagt
      721 atgtatcaat caatggcgga aatcatggag gttttggcag ctatggtgag caaaaaggag
      781 atggagaggc agaaatttct aacgcagacc agcaaactgt gatcgtcaat acattggaaa
      841 actggctgaa aaagttgaaa taatataaat gggtcctata gggtccgtga aaaaactagt
      901 caattaacta tagatagcaa atgaatcttt caaaataagt cgggatttca cggaacaaga
      961 gaaacagatc ccggcttatt ttcaagttta gagaacttag tgtacgtttt taaagtttcg
     1021 tttatattta tattttattt tctaataaaa tcgctcgaac tggacatttt tgatacgctt
     1081 tttttacata tgtttcttct tccggcatca cgaactgttg atgagcctct ggttcttggg
     1141 caaataaaac gattccttct tcatcataat cgaaaatatc tggcgcttcg atttgacaaa
     1201 gaccgcatgc aatacagcgt tcaggtacaa gtttacatag tagtgacatt tctaaccatc
     1261 ctaacttaag gagaataata tgattgtttt tattttatcg ctattttcat caggacacaa
     1321 gctgcggatt agttctcttt accaactcct tgtgggaaaa cgaacgactt cagtacttat
     1381 ttatggattt acccacgaac ttttgtttat ccataacagt tttccagaat tgaagcagga
     1441 taagttttat caaattttgc aaaaaattgc gcagcaaggt tggatcgaaa taaatgaaaa
     1501 tgaagcaaag ctaacgcctg caggggctga tatgttgtcc gaacatcaaa tagaacatac
     1561 tggtttgcgt tttgaccgct atggcagaac aggggaaact agttggcggc tcattaaatt
     1621 tgctgttcaa gtaatttcta atctggcaac gggtattcaa gactatcttc cagcagagac
     1681 aagtcctttt tatactttcc aactaaaaaa atggcttagt gaaagccaat tgccacgtga
     1741 aattttgatt gatactgcgt atgagagttt ggtgcagctg ttttcggaga taccagaaga
     1801 agcagcagat tttttagcta accaattatc tggaaatgag cgaacagggc tgcttcctta
     1861 tcagctggca aaaaacaatg atgagtcaga ggtttatctg caacaaagca gatgtatcca
     1921 ccttttgtta gcacaaatcg aaaaacggcc agattcgctt tggcacgctt tgatcgattc
     1981 attattacag caaaatttca atcaaagcat gatgatcacc aagcagatgt ttatgaatgg
     2041 tcagacaatt gaccagatca tggcaatccg tcatttaaaa agaggaacag taacagatca
     2101 tcttattgag tgggccttgt tttttgatga ttttccatac gagtggattc tctctgcaga
     2161 aacaatcgaa cgtttggaac caaataagga ttccgtgcga gagtggcgct ttagcgaatg
     2221 gaatgtagat ggacaattag attatggaga atttcgtttg tatcaaatct atctattgag
     2281 aaaggaagcg atacagaatg ttaataaatg aattaaaaaa atattttgga tttgattcat
     2341 ttcgtcccgg ccaagaagaa attattcagg cgttgcttga tggagaagat actctcgcta
     2401 ttttgcctac aggaacggga aaaagccttt gctatcaatt gacggggtat ttaatggaag
     2461 gtctggtcat catcgtgtca ccgctgcttt ctctaatgga agatcaagtg acgcaattac
     2521 aaaaacgagg ggagaaacga gtagctgctt tcaatagttt actttcgcaa tctgaaagaa
     2581 gatacgtcct ccgtcatcta tcgcaatata aatttttgtt tttgagtccc gaaatgttga
     2641 ctaatccttc tcttttagag catctgaaaa aacaaaatat tgcgttatat gtagtagatg
     2701 aggctcattg cgtgtctcaa tggggagttg atttccgtcc agaatatcaa cagttaggaa
     2761 aaatccgaaa gcttttagga aacccggtca cactggcact aacggcgaca gcaacagatt
     2821 tagtagcaag agatatacgt catgtactat ttgatcgacc accaaaagag atcagacaat
     2881 cagtcaatcg gcaaaatatc tcgctttttg ttcggaaaac gcagcaaaaa gaacaagaac
     2941 ttgaacactt catggaaaga gcacaaggag cggcaatcat ttattgtgca acaaaaaaag
     3001 aagtcgaacg attgtatcat ttattcaggg aacgttttac agttggatac tatcacggtg
     3061 gtcttgatgc agctcaaaga agacaacttc aacagcagtt tgtaaaaaat cagctgcagt
     3121 ttctcattgc tacgaatgca tttggtatgg ggatcgataa atctgatatc cgttatgtgg
     3181 tccattatga tttgccagac agtctagaaa actatgtaca agaaatcggt cgagcaggca
     3241 gagatcagga agaaagtgca gcaattcttt tttatcaaag tggggatgaa cgtatccatt
     3301 atttctttaa tcaactttct agagaacagc gacaaagttt tgagctttat ttggaatatg
     3361 ccacaaagca agctcccttt gatgagctgc aaaaaaaatg gatggagctg atccagcagt
     3421 ctgaaaaacc agaaaattgg gtggaacggc tcaaacgtca ggaaaaagaa aaagaattcc
     3481 gcctgcagca gatgcttcgc tatattaatg aagaaaactg ccgcagaaaa ttcatcttag
     3541 cttattttgg cgaaaaactc tccgaaaagc cgcagaattg ttgtgatatt gatggagcaa
     3601 aagcagaact tctgccaaag aggaagcttt tttctaagac tgaacaactt cattgggaaa
     3661 cgattttact aaatttattt aaaaaaaaca tggaacattg attttttagg acaagacact
     3721 ttttcctatg gtataataca cacgatagag tttaggaggt aaaacagtga gcagaaaaga
     3781 tagacatcaa tctacagaaa ataacgaaac acaagaacca tgggaacaac caatctacga
     3841 tacagatgat gaagtttcat caagaagttc tcagagacaa caaaagaaag gaaatacatt
     3901 atttctttcg ctatttttga ttttactggt cttatgtatc gctattccag caggtgctta
     3961 tttctggatc agaaatggat caagcaacaa tgcaacagca gcaacttctt ctgaaacttc
     4021 gacatcagta gtagaaagtt ctacaagtac aacaaaagag gagtcaactg agacttcaac
     4081 agtagaatct agtacaatcg cggaagaatc tactgtgtct caagatacag gtgtaacgtc
     4141 agaaagcagt attgcagcag agacaacacc gacttcctca agtgaggcaa cttatcaagc
     4201 aggggattca agcagcaatg cagcagaaac aacaccttct tcatcagctg cagccggttc
     4261 aacaacgact gttcaagcag gcgaaggtcc aaaacaagtg gctgagcgtg cagggatcac
     4321 cactgatcaa ttgtttgaat tgaatggctt agatccaaac aactttatgc tttaccctgg
     4381 tcaagaatta agaattaaat aataatagcc gcgctttgcg gctttttttc tgagaaaaag
     4441 gagttatatc atgacacaaa tcagtattgc aatcgacggg cctgcttcat ctgggaaaag
     4501 taccgttgcc aagatattag caaaagaatt gaattatatt tatacagata ccggtgccat
     4561 gtatcgcgca gtaacttatt tagctattac gaacaatgta tctttttcag atgaagaagg
     4621 gctagtttcg ctgatcaaaa agtatcctat ctctttttcg caacaagaag atggacagca
     4681 tgtattcata gcatcaaaag atgtgacttt agaaatccgt caaccagatg tcacaaaagc
     4741 tgtttctgaa gtctctgcac atggaaaagt cagagaagaa ttagttgctg tccaacgaaa
     4801 aataggggaa cgcggtggcg ttgtcatgga cggaagagac atcgggacag ctgtattacc
     4861 gaatgcagaa gtgaaaatct ttttagtagc aagcgtaagt gagcgtgcag aaagacgttt
     4921 caaagagaac caagaaaaag gaattccgac tgatcttgaa acactgacga aagagataca
     4981 ggaaagagac cattatgatt caacaaggga cgtctctccg ctgaagcaag cagaggatgc
     5041 tgttcgtatc gatacgactg gcaaatcgat tccagaagtc gttgcagcaa taaaagctgt
     5101 tgttttgaaa aaaggttatc ttctttttta aattaagaaa aaaaatgaaa aacaattgtt
     5161 ttttcaagga aaacgaacga aatcgcttta ttttttttga ataatcaatt aaactaaaaa
     5221 cagtaccgta cttacggcag aatgttggtt aggaggacaa gtgttatgac agaatttgat
     5281 caaaatcaag agaacaacca gtcaatggaa gacgcaatga aaagcgtaca agaagtaaaa
     5341 gttggcgatg ttgttaaggg cgaagtttta gtcattgaag acaaacaagt gattgtcgga
     5401 atcgaagggg ctggagttga aggagtcgtt ccggcaaaag aattatcaac tttgccggta
     5461 gaagatatca gcgaagtagt aaaagtcggt gatgtattag atttagtcgt tatcagtacg
     5521 atcggcaaag ataaagaaaa cggcagctac ctactttcaa aacgccgttt agatgctaaa
     5581 aaagtttggg aagatatcga aagagatttc caagagggca aggtcatcga agcacctgtt
     5641 acaaatgttg tcaaaggcgg tttagtcgtt gacgtcggtg tacgtggttt tgtccctgca
     5701 tcaatggtgg aagatcattt tgttgcagac ttttcagact ataaaggcaa aacattgagc
     5761 tttaaaatca tcgaaatcga accatcagaa aatcgtttga tcttatcgca taaagcggta
     5821 gtggaaaacg aaagagaaga aaagaaaaaa gaaattcttt caactatcca tgagggagat
     5881 gttctcagtg gacgtgttgc acgtcttact gattttggcg catttgtcga cctaggcgga
     5941 attgacggtc tagtccatgt ctcagagatt tcacatagtc atgtagcaaa accaagtgat
     6001 gtattggctg tcaatgatga tgtaaatgtc aaagtgttgt ctatcaatcc tgaacaagaa
     6061 cgtgtttcct tgtcaatcaa agacacacag cctggacctt ggtcagaaat cgatacaaaa
     6121 gctccagttg gcacagtatt agacggaaca gttaaacgtt tgacaagctt cggtgccttt
     6181 gttgaagtgt tccctggagt agaagggcta gtccatatct ctcaaatttc acataaacat
     6241 attgcgacac cacatgaagt gcttcatgaa ggtgacgaag tgaaagtgaa agtattagag
     6301 atccatccag aagaacatcg tattgcattg agtatcaaag ctttagaaga aaaaccagct
     6361 tcagaagaag aaccaaaaaa tgttgagtct tatgaattac cagaagaaaa tactggattt
     6421 actatgggag acattttagg tgatgcgttg aaagaagaag tagaagaaaa agcaaccgat
     6481 gattctgaaa aataattgat tttgtatctg tgactgagat gaaagcagct gcttttgtct
     6541 cag
//
LOCUS       DAFEHS010000109         6411 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFEHS010000109
VERSION     DAFEHS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.114"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            76..1380
                     /locus_tag="LP327_002578"
     CDS             76..1380
                     /locus_tag="LP327_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL1726648.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1472..1837)
                     /locus_tag="LP327_002579"
     CDS             complement(1472..1837)
                     /locus_tag="LP327_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL1726649.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1834..2199)
                     /locus_tag="LP327_002580"
     CDS             complement(1834..2199)
                     /locus_tag="LP327_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL1726650.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2391..2639)
                     /locus_tag="LP327_002581"
     CDS             complement(2391..2639)
                     /locus_tag="LP327_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726651.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2638..2766
                     /locus_tag="LP327_002582"
     CDS             2638..2766
                     /locus_tag="LP327_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBL1726652.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2823..4517
                     /locus_tag="LP327_002583"
     CDS             2823..4517
                     /locus_tag="LP327_002583"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBL1726653.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4521..4640
                     /locus_tag="LP327_002584"
                     /pseudo
     CDS             4521..4640
                     /locus_tag="LP327_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5168..6301
                     /locus_tag="LP327_002585"
     CDS             5168..6301
                     /locus_tag="LP327_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBL1726654.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
BASE COUNT         2121 a         1222 c         1169 g         1899 t
ORIGIN      
        1 tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa agctctccaa tctcctgcat
       61 gtttgcaaaa ccactttgct caatgatatc aataacataa aaaaagaatt tgaagaacag
      121 atcgcttata cacatcggga aaaggaccgt tacactttat atttttcaga acatatacct
      181 cgatgcaaga tcatgcagca attgtctcaa aattcgttgt ttttaaaaac ttgtctgctt
      241 tatttggaag aagatgaacc tgattatctt caattaactg aatgtgaatt catctctgtc
      301 agcaaagctt attcattaaa aaaacaagta ttagcttatt ttaatgactg cgggatcgaa
      361 atcgaccgct actcccctcg attcactgaa atggaaagac gtctgctgct tcttaatgtg
      421 tcgtatcgtt taggcggttt taattcttgg gagctacctg aatcgttttt tgaacgtgcc
      481 gatcgtttca ttgaatccgt aacggaaaat agtggcagat tttatgataa agaaaataaa
      541 gaaatcttat tgattggttt tgctatcagt ttcctacgcc agcaagtgtg tgctgtgaca
      601 atagattcaa aatttatcga agaaatcaaa aaacgtccta tttacaatta tgtcgaatca
      661 gcatgggaaa acactgattt ccaaacctat tataaaaaag aagaattcgc ctttattttg
      721 acactattca atctttgtaa ttatggtttc cattcttatc agttgattgc ggaagacttt
      781 caacagctac accaagtgtt tattgacaat actcccgaaa taaaagaact agttgctacc
      841 tttgaatctc acttcaatca agaattattc ggaaaccaac cattcgaacg tgcgctgatt
      901 catctgatgc gttcggcttg ggataactat caattattca tgccagaaaa attttatctt
      961 ctaaatgagg aacagacaaa tttatataaa gaagtccaga caattttttc cagctggtcc
     1021 agccagttac cttatgatct acggttgaat cctaattgca tgcgggcttt tgtgatcgaa
     1081 ttgagcggaa ttctccgtct gacaaaagaa catctgacta tctatatcgt cacaaattcg
     1141 gatgttcatt atctgattta tcgggaagcc ttggaagctg taactacttt cgattttcaa
     1201 gttgcaccaa caatttattc aagtatatct gatataaaga agtatgcgca gcagtcctca
     1261 aaccgggtat tatgtgaacg tacattatat acaccagatg cggtccaata tgaaaatatt
     1321 attcctattt cgatcaatac gatcgatcga gctatcattt cagctgtgca aaacaaataa
     1381 gggactgtga caaaaatcat gtcacagcct cttatttgtt gaagatgccc atttctgtaa
     1441 caatctcttg atacacatca ggctcacaac ctcacttatt ttggacatct acgggtccga
     1501 tctctttatt taaaaaataa gaaatgatgg ttcggataac taccaacgcc gctagttgga
     1561 aaatatcctg caaggtaggt ttgataatcg attcgatgat cccagctgaa acaagaattt
     1621 ccaaacttag aagtatatag cctcccaaca tctttttaat agcattgttc tcgctgactc
     1681 ggtcgctttg cacttttgta tggacaaata aatatttcaa aaaatccttg attgatagac
     1741 aaacacccca aaccaataaa cagatagcaa agatatcaag tcccagtaca agtttttcaa
     1801 aatatggaag aagaaactgc atcaactctg tcgtcatcct acctctcctc ttctttgttt
     1861 ttctttgtct tctatttcct gattcaagaa ataagaaatg actgttcgaa tgataataat
     1921 aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata actgactcga tgatatcagc
     1981 aacgactagt acttccaagc ttaacaaaat ataacttgct agcagttttc ttgtatctgc
     2041 attacgagta ttacgttctg catagttttt cttgacatga gaccaatcaa aaagtttgaa
     2101 tagaacaaga acgatcccga caattaagat accgatagaa caaatattta aaatagcaat
     2161 gatccattct agaaattcaa ttaaatttga gcgaaacata tacgctacct actttctttt
     2221 aatttgttcc tattctttta ttgtaaaaga aagttacctt gttcgtctat ttaaaagttg
     2281 ttctaatgat aacggttcgc ttaaattttt ctttttcact ctcccctttt tctccctcca
     2341 tccataaatc gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc ttagtccgtc
     2401 atcagctttt caaagaagca ctgtctcggt gctttttttg tacccaaatt tctaaaaata
     2461 gaaccataca cctcctcaaa tcttttcttc ttacctaccc tatttttatt tacaggaaaa
     2521 tcagacactt cttctttttt gtcaaaattt cctttttctt ctcgtcctat catttggcat
     2581 tctaataaaa acgaactatc cttggatata gaaagagaaa acaaggaggc gtttttcatg
     2641 agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg cgctgcatat
     2701 ctaggaaaaa agacaggact attcgaagat gacagtcatt tatatgatga attcgaatca
     2761 atctagcatc ctatttagtt gaatcactac ggacatactt ggaagacatt ggaggaatca
     2821 caatgagaaa aagtaaagca ataatgattg gtgccggtat tgccaatatg gcagcggctg
     2881 tttatttgat tcaagaaggc aaatggaaag gggatcagat caccttttat tcattggatg
     2941 atcacggttc aaatgatggt gcaccgaccg acactgtagt ggatgaatat tggaataaaa
     3001 accatccatt agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg cttaattacc
     3061 gtacctatgt agatttgatg gatctgttaa gtcgaatacc ttctgttact gagccaggga
     3121 tgactgctga agaagatacg agagattttg atgcaaaaca ccgaaccttt gacaaagcac
     3181 gtttgatgga aggtggcaaa ggaatcattg atggcggaaa actgggattc aataataaag
     3241 accgtattct tctgactaaa ctgattgcta tgcctgattc agaagaagaa aaactagaca
     3301 atatcactat cgaagaatat ttcaaagatg atccacactt ttttgaaaca aacttctggt
     3361 ttatgtggga aacaacattt gctttcagaa cacgtagttc tgctcaagaa ttgcgtcgct
     3421 atatgcatca aatgatttat gaattcactc aaatcgaaca tctagtgggc gtcaatcgaa
     3481 cacgttacaa tcaatacgaa agtattatgc ttcctttgat cgagtacttg aaagaacaag
     3541 gatgcaaaat cattttgaac cgccgtgtca ctgactggga attcaaagaa acaccgatgc
     3601 aagatgaaat cactgtgact ggtttgaaaa tgaccaatac actgactcaa gaagaagaac
     3661 acgttgtggt cgatgaagat acagcagtta ttttcactaa tgggtccatt acagattcag
     3721 ctactttagg agattacgaa acaccagctg tagaaaatat ggattatggt gcagcttcta
     3781 gtctctggaa aaaagccagt gaacgtttct acaacttggg aaatcctgac aaattctttg
     3841 ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac catttgttgt
     3901 tgaatgaaat cacccgtatc acgactcaag aaccaggaaa tgccttgaat tcatttattt
     3961 caaccacacc gatcacgccg ttaggccaaa aagatgtcaa tatgtctatc gtcgtgcatc
     4021 atcaaccgca ttttacgtct cagaaaccaa atgaaacagt tatttggggt tatttccttt
     4081 atccaagacg acgtggcgaa tttgtggata aacagtatat taaaatgaat ggaaaagaaa
     4141 tgctagaaga actgattgga caactttcaa aagtagatcc tggaccagtg aatattcgtg
     4201 aaaaagaaac agaaattttc gacagcgtaa taaataatat ccctgtctat atgccttatg
     4261 cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt cctaaacatt
     4321 cgaccaatct ggcctttaca ggagaatttg tcgagcagcc ttatcagatg atctttactg
     4381 aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt atccctatgt
     4441 cacgtcttgt caaaacaccg cgttacgaca aagatattcc tactctagct cgtgccgcaa
     4501 aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     4561 caaccaggct aaaacagctt cttatgtttt tgatttggtt ggacttatta ccgcagatgc
     4621 aagcttttga acaccgttta ccaagaggtt gtgaaaaagc tgccctgcat caagtaataa
     4681 gagcagccaa gctaaaacag cttcttatgt ttttgacttg gttggactta ttaccgcaga
     4741 tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa agtcgtttag tttcgagtat
     4801 gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca taaatttgac ttaataccga
     4861 gaaactgatc ccttctgtca taacctcttt ttatttatgt ctgtttcttt ttgaaaacaa
     4921 ccattttttc aacgtttact ctcttgccaa tccaacatca tctaacagat acaactattt
     4981 caatgattac cattacacta aaaaatcgga aactcacctc tccgaacaca aatacataag
     5041 actatagaat gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt aagatctcct
     5101 ttaaaatgaa attatctgta gtactaataa tgcagacaat tttatgaatc tgaaaggagc
     5161 atctccaatg aaagcagtca cttggcaagg aaaacagaaa atggaaatca gaaacgtaga
     5221 tgatccaaaa atattagaac caacagatgc aatcatccgt atcacagcaa cagccatttg
     5281 cggctccgat ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag ggtatgttgt
     5341 aggacacgaa ccaatgggaa tcgttgaaga agtgggatct caagttcaaa cattgaaaaa
     5401 agggcaacgt gtggttatcc cttttaatat tagttgtggt cattgtcatt tctgcctgaa
     5461 tcacatggaa agccaatgtg atgaatcaaa tccagatcag ttatatggcg ggcttttcgg
     5521 atttggcaag ttgaacggta atcattgggg tggtcaagcg gaatatctaa gagttcctta
     5581 tgcagactct acttcatttg ttgtaccaga taatgatctt cctgatgaaa aagttttatt
     5641 tctatcagat attttgccca cagcttattg gagcgttgaa aacgcaggag taaaaaaagg
     5701 cgatacagtt gtgattctag gttctggtcc agtcggtcta ttcgctcaaa aattcgctgt
     5761 tatggctgga gcagagcgcg taatcgcagt agatccagta cagcaccgtc tagataaagc
     5821 ggctaattac aatggggttg aaacttattt cttagaagac acggcacaag ctggaacaga
     5881 attgtatgaa ctggctaaag gtggcgcaga tgtcattatc gattgtgtag gaatggacgg
     5941 attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaaacg gcacaatctc
     6001 tccattacag atggcatcgc aagctgtcaa aaaatttgga acagtccaag taactggtgt
     6061 ttatatgact ccagcttctt cttacccgct gcaagaattt tttatgcggg atatcgctgt
     6121 caagcatgga caggcaccaa ttatccatct aatgccaaaa atttacgaaa tgattgcaga
     6181 aggactattc gatcctacac aaatcattac tcattcgctg ccactatcag aagcagcaca
     6241 agcatatgag atctttgaca aaaaagaaga taaaaatata aaagttgttt tgaaaccttg
     6301 aattcagtaa aaaatacagg tgattttctt tatgcccaaa atcaaacgaa atacccatga
     6361 gatcgaaata ggaaaagaag aagatatcga aatacgagga cgtcgcttcc g
//
LOCUS       DAFEHS010000110         6287 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFEHS010000110
VERSION     DAFEHS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.116"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..177
                     /locus_tag="LP327_002586"
     CDS             <1..177
                     /locus_tag="LP327_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726655.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="LP327_002587"
     CDS             190..690
                     /locus_tag="LP327_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL1726656.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="LP327_002588"
     CDS             787..2634
                     /locus_tag="LP327_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBL1726657.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="LP327_002589"
     CDS             2637..3533
                     /locus_tag="LP327_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL1726658.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="LP327_002590"
     CDS             3583..3972
                     /locus_tag="LP327_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL1726659.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="LP327_002591"
     CDS             3959..>6287
                     /locus_tag="LP327_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL1726660.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAFEHS010000111         5880 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFEHS010000111
VERSION     DAFEHS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.117"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(1..191)
                     /locus_tag="LP327_002592"
                     /pseudo
     CDS             complement(1..191)
                     /locus_tag="LP327_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            212..1414
                     /locus_tag="LP327_002593"
     CDS             212..1414
                     /locus_tag="LP327_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HBL1726661.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2147..3115
                     /locus_tag="LP327_002594"
     CDS             2147..3115
                     /locus_tag="LP327_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL1726662.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3126..3941
                     /locus_tag="LP327_002595"
     CDS             3126..3941
                     /locus_tag="LP327_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBL1726663.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4009..4719
                     /locus_tag="LP327_002596"
     CDS             4009..4719
                     /locus_tag="LP327_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL1726664.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            4755..>5880
                     /locus_tag="LP327_002597"
     CDS             4755..>5880
                     /locus_tag="LP327_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBL1726665.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         2018 a          884 c         1188 g         1790 t
ORIGIN      
        1 gttttaagca actgacaata gctggcattc atgtccatca ctaggtactt gacctttaaa
       61 cgggcctttc taggaaagcg caggaagtat tgagttagtt tgtaaagtct gcgatcttca
      121 agggtcgtaa atagctgatt ggttgtccca tcaacacaaa taaaactcat ggaaccagaa
      181 gtggcaacat tgtgtacttt tctttgcaat cttgtgtaca aaactattgc aaaacacagc
      241 gcataagaat ccttacaaaa cagtagatga agcaacccaa ttagatcttg ttatagttgt
      301 taatcagctc ttaacgggtt acgattcaaa atgggtcaac accctatact tagataagtc
      361 acttgaatat cagaacataa ttcaggcttt ttcacgcaca aatcgtctta atggtcctga
      421 aaaaccgttc ggaaccattc gttattatcg taaagcacat acgatggaac agaacattga
      481 agaggctatt gagttatact caggcaaaca agcaaaaggg ttatttgttg aaaaattata
      541 taataactta gaaaagttga acgatatatt cgaagaaatt aagtccgtat ttaatatttc
      601 tggcatagaa aacttcagtc atttaccgga ggttccaggg gaaattaagc aatttgtaaa
      661 tctttataat caactaatcc attgttatga atcagcgatg atacagggat tcacatgggc
      721 aaaatcgact tatgaaatcc aggattatga acctataaca gtagtaatca cagaatatga
      781 atttaatatc ttggcacaac gttacagcga gatagagaga gaacctagag acggaaatga
      841 tagtatagcg tatgacattg attaccaatt acctgaaggt agtcgtagtc gtattaatta
      901 tgagtattta aacacaaact tcaaaaaata catgaatata aaagaagaac gtgattcgaa
      961 tttaattcaa tcattgttaa acgacttaca tcgcacattt gcgcaattac cagtggagtt
     1021 acagccatat gctgagatga ttctaaatga tgtaaataat ggggaattag ttatcaaaga
     1081 aggatgggaa tttactgatt acctcaatga atatcagctt agtgaagagg acgattttat
     1141 ccaagattta gtggattcaa ccaatattaa tgaggcatta ttacgtgaga tgttggattt
     1201 acgcttaacc gaggtaaaca ttaatgaata tagtcgattt gataaattaa aatcaagtgt
     1261 taacgtaggt catttcagtg gatatatcga gaaaaattta ggtaaaatgg taataccaat
     1321 caaggtaaat atgctaattg acagattatt gcgtgcattt ttattagaag atgtattcga
     1381 tgttacagag tacataaaga attactttaa ctagagtaca cctacatctt atagtacata
     1441 aaaaaattca tcttttttag tttaataatt caccaaaaag gtgagtaatg ccatttttga
     1501 aaattgagct tgttttatgt ctacatctgt atccgtatat aatccaaagg attataaact
     1561 cagaagagtc tttatttttt ctgaatataa ctactgtatc aaaaacaatc gattatgaga
     1621 caaaacaaaa tgacctcttt tatttaagaa aggaggtcat tttttgtatc ataaattgta
     1681 ttaaaatcaa tgtatcactt tttaatcaat tttaagacca taaattgcgt actaaaagaa
     1741 attctgcaat tcaaaataaa gcattttaag ccattttcaa atttaagtgc acccatctac
     1801 ccgtcaaatc actttatttt ccactccagt agtttcctaa ttcaactgtg tgcaaaacga
     1861 tttcttctaa acaaatcaag acaaacttat aaaccttcat cccagcacca taattattta
     1921 aatcataggc atttttcgaa attagtatat gtactcaagt ttcactgcag attatttata
     1981 ttttttagag aaataaattt ttcctattga caaacacaaa gaaagcggat acaatacaat
     2041 tatgtcacat gacgttatat gacgttatgt aatatcttta catttaatca gttactttta
     2101 ttttggataa aattaacatt tttaaaattt taaggaggaa atgttgatgg atgtagcaaa
     2161 aacggttaac gcaattgtag aaaaaaatga tggagagatt tctggaattt atttggtggc
     2221 ttgtggcggt tcgctggtag atatgtatgt ctcagattat tttcttaaag ccgaagcatc
     2281 gaagtgtatt acaggcttat atacagcgaa cgaatttaac catgtaaccc caaagcaatt
     2341 agatgataaa tctgttgtca ttctatgttc tcatggagga aatacagcag aaacggtaaa
     2401 agcaggagaa gtcgctcaag ctagaggagc tgtgactgtt ggtctgactc ataataaaac
     2461 tgcggattta ttaaaggttt ctgatcatag tttcctttac gaatggggag atgatgcaaa
     2521 aatagtcaat aatcctatgg ccatcattct tgatttaact gtatctttgg tcaatgttac
     2581 cgaaacattt gaacattacg gtaaattcag agaaggcatg aaggttatcg ataccgtaat
     2641 tgaaaatggc attaagcagg tgcaggatcg tattaaagtg tttgctgata agtacaaaga
     2701 tgaaacaatg ttttatatct taggtagtgg accatcatat ggacatgctt atggtttttc
     2761 aatttgctct ttgatggaaa tgcaatggct tgattcaact tctgttcatt ctggagaatt
     2821 cttccatgga ccttttgaag taacagacaa agatacgaat tttattctct tgatgagcat
     2881 tggacgcact cgtgctttag atgaacgggc aaaacgattc cttgataaat atgcagaaag
     2941 agtagaaatc gttgatgcaa aggaactagg attggatatg atatcagatg aagtttctga
     3001 attcttcaat cccattttat tctacagtat tctttcagaa tatcgttcag ctttagcaga
     3061 tgcacgcaat catgatttag atgttcgtcg ctatatgggg aaagtggagt actaggagga
     3121 ttaaaatgaa ggtattaggc ttaggtgata acgtcgtcga taaatatgaa aatctgggaa
     3181 ttatgtatcc tggaggaaat tcattgaatt ttgcagtttt tgcaaaaaaa ctgggacatg
     3241 aaagtagctt tatgggagtt tttggctcag atgaggaggc agctcatgtt ttatcagcaa
     3301 taaaagaaat aggactggac aacagtcact ctcgctttga aaaaggcgag aatggctgtg
     3361 ccagagtgaa gattgatgag ggcgaccgga ttttccttgg cagcaattcg ggaggtgtta
     3421 ctcgtgaata cccgattcag cttactgaag aagacagaga atatttaaac cagtttgaat
     3481 tgattcatat gggattatat agccatgtca atcatctatt agaagagtta caaaatgttt
     3541 ctggaaagat ttcttatgat ttttctgacg actttacgga agaagaggtt cagcgggcaa
     3601 ttgataaagt tgattttggc ttcttttcta tagaagactt ttccgatgaa gaagtaaagc
     3661 agtttttgat gaagcatttt tgtgagcgaa atgatgtatt gattgcaacc cgaggagaaa
     3721 aaacggctat tggttatgat ggagcaaagt tttatgaagt gaaccccgtg ctagaagaac
     3781 cagttgatac aatggcagcg ggagattctt tcttaacagc tttcctactt tattttctcg
     3841 aaggggacga tatagtcacc gcgatgaaaa aggggaatga atttgctggg aagtcttgtc
     3901 tggttgacgg ttcgttcggc tttggtattc attacgattg agcttgtttt aaattcctgc
     3961 atttgataac ctaaagaaag aaatcccggt aaagtggagg ttaaaaaaat gctggataca
     4021 aatgcacaat taccattgta cttacaatta aaagaaatat taaaaaataa aatcaataca
     4081 ggggagtatc ccgagaatac gaaaattccc gctgaatcag agctgataga aagttatgag
     4141 gtcagccgaa tcacagtgag aaaagcagta gaagaactgg tagaagaagg gtacctactt
     4201 aaaaaacaag ggaaaggaac ttttgtcaac cggcataaag tttttcgtaa aattgaatat
     4261 gttatgggtt tcagtgagag ttgtaaatct aatggtttta caccgactag cattgttttg
     4321 gaacgcaaga tcattccagc aactgaaggg atggcaaaaa aactcgatat cgctcaaggt
     4381 gatgatgttt tatatatcca gagaaagcga atggcggata acatccccat tatgttagaa
     4441 aacaattatt tttcttatga aagatatagt ttcttaatgg aggaagagct gactgattct
     4501 ttgtatgaag tacttaaagt ccaaggtacc ttagccatca atcctgggga aactgcactt
     4561 gaaattgtca cagctgatga ccaaattgcc aaaataatgg aagtagctgt tgggacgccc
     4621 tttttcttta tggatacttt aatcaacgat caaaacaaca taccaattca tgtaggtaat
     4681 caatattacc tgggagaagt atatcgtttt tacttatgat ttcctgtttt gatttcaagg
     4741 agtagatttt atttatgaag caaatattgc ggtcaaaaat gatttttgat ggagagcagc
     4801 cacctttcaa aggttatatt gtaatcaatg aagggatcat tgaaaaggtg gaaaacaact
     4861 ggaattacag tgatctatta aatgaaaaca cggaattaat tgcttatgat gatcaattcg
     4921 ttatgccagc aattcatgat aatcatgtat ttttcagtgg ttatttggct atgaatgctg
     4981 gcatagattt aagcgataca tcttcagcta acgaggctgt agagcgtatt aaagctgtaa
     5041 tgaaccataa aaaagcagat gaaccaattt atgctcatgg ctgggatgct gccctgtgga
     5101 aaactgaccc atcagaagag ctattgaatg aaataaacca ctttggtcca atcacagcga
     5161 tcgacaaaaa tcgcagccat tgttggatga atgctattgc taagcagact tatggtttta
     5221 cagaaaaaga aaccagtgct gaaagtcgag tattattaat taaggaaatg ctagcaaaca
     5281 aaaagctttt agcagaggtt tacgctgaat ttgaagcatt actgctttca aaaggtgtta
     5341 tatcaatgaa agaaatcgtt tttgatgacg cagaatttct ttctaatata cctcagagga
     5401 aaatacttag taacctatat gtgcaggcag tccaagaacc tctacagcat gaacttctat
     5461 atgagtataa ggaaaaagtt ttccctgaaa aggtccgctt tggtggagtg aagatcatgg
     5521 tagatggtgt tgttgcggat agaagtggag atatttacgg tgtttatcag tcaggagtag
     5581 cttcacccga gattgactat gaggcaatta aagcagaagt tgctgcattc aatgaagtag
     5641 agattccttg ctgtttgaca acggaaggag ataaagcggg ttataaagct gcgtcaattc
     5701 ttgtcgaatt tggaaagcgg ttacctgaag gcgtttttaa ttcgattagt gatttggaaa
     5761 tggttactca aaagacagca gaaaaaatga acgaaggaca agtagtagca gaggtatatc
     5821 cacaaattct cggcttgaat caaacgttag aagaggctta catgtcaact gttataaaaa
//
LOCUS       DAFEHS010000112         5875 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFEHS010000112
VERSION     DAFEHS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.118"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            641..1375
                     /locus_tag="LP327_002598"
     CDS             641..1375
                     /locus_tag="LP327_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL1726666.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="LP327_002599"
     CDS             1391..2560
                     /locus_tag="LP327_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL1726667.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="LP327_002600"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="LP327_002600"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL1726668.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="LP327_002601"
     CDS             3650..4576
                     /locus_tag="LP327_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBL1726669.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="LP327_002602"
     CDS             4595..5845
                     /locus_tag="LP327_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL1726670.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1203 g         1750 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaa
//
LOCUS       DAFEHS010000113         5824 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFEHS010000113
VERSION     DAFEHS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.119"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..1253)
                     /locus_tag="LP327_002603"
     rRNA            complement(<1..1253)
                     /locus_tag="LP327_002603"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1939..3444)
                     /locus_tag="LP327_002604"
     CDS             complement(1939..3444)
                     /locus_tag="LP327_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL1726671.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(3441..4127)
                     /locus_tag="LP327_002605"
     CDS             complement(3441..4127)
                     /locus_tag="LP327_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL1726672.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(4320..5741)
                     /gene="gndA"
                     /locus_tag="LP327_002606"
     CDS             complement(4320..5741)
                     /gene="gndA"
                     /locus_tag="LP327_002606"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBL1726673.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1541 a         1325 c         1037 g         1921 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg cctttcaaat caaaaccatg cggttttgat tgttatacgg tattagcacc
     1081 tgtttccaag tgttatcccc ttctgatggg caggttaccc acgtgttact cacccgttcg
     1141 ccactcctct ttttccggtg gagcaagctc cggtggaaaa agaagcgttc gacttgcatg
     1201 tattaggcac gccgccagcg ttcgtcctga gccaggatca aactctcata aaaagttcga
     1261 acaatcttgc gattgttaag ctcaatttgt ttgctagcat attgcttgct tgttaaaaat
     1321 gtttgttgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
     1381 gttttcaaag gtctaatctt tttgttttaa caactcttta atcttatcag attcagaaga
     1441 gaatgtcaac aacttctttg tttatttttt ctaaacgtct ttgccactca ttagcagcga
     1501 cttgattatc ataccatctt tattgttatt tgtcaataac tttttcgaag tttttttcga
     1561 taaaatcaag ctttattagc tatctcatcc aaacgacttc gttattataa catgacatca
     1621 aaagtgcgtc aacactattt tttataattt ttagtgacac cttaaaggaa tcatttagaa
     1681 caaataataa tatagcaatc tctttataaa attgcaatag aaaattccct atttttattt
     1741 attattttta aacacgaata ttacagcatt ttattgtttt ttcgttcatc taaaagatac
     1801 ttacatttaa ttaacggatg ttattctatt taattgttcg ctagtttttt tataatgaca
     1861 tccctgaatt tctcacttgc ttgtattttg taccttattc tttgttggat atataaaaaa
     1921 acaagtaaag cacatgcttt acttgtcttc tgcctttgcg ataggaataa agattctaaa
     1981 aattgtccct tgccctacta cactttctgc taagatacgt cctttatagt tttcaaccag
     2041 ttgtttagcg atagacaacc ccagaccatt tccacccttc gttctagctc ttgctttatc
     2101 cactcgataa aaacgattga agatcttatt aaggtcttct ttgggtattc cttcgccaaa
     2161 gtcctgaatc gcgatctcaa attcattcag agtggaagaa atagagatat ggacttcttt
     2221 tcgtgtagtt gaatacttta ctgcgttatc caatatgata atgattaatt gttcaaagtg
     2281 attacgataa atttgcaggg tcacttcttt tgtcaaatca tcatctaaag tgatcacata
     2341 gtctggatat aatatcttga aattattata aacttgatag gtgacttctt ttcctttagt
     2401 tgtttcattc gcataatgaa catctacttg ttctgctctt gataagtcta gcatctcttg
     2461 aactaagctt ttcatacggc tgatttcttg catgctggcg cttaatgatt cttctaatat
     2521 ctcaggatca tctttccccc aacgatttag cagactaaga tgcccttcaa taattgcaac
     2581 aggtgtcctt aactcatgag atacgtcttc cacgaattgc tcttgttgtt caatatacaa
     2641 acgcatacgg tcaagcatct cattgaatat ttcagctaaa tctgccaatt catcatttgt
     2701 gtcgatttca ggcatgtgga tatctgattg cggatcttta cgtatagtat ccattgtatc
     2761 tctgagtacc ttgagtggtt ttaagaaata agaagataaa aagaaaccta aaatactact
     2821 taagatcaaa gaaacaactt ctaaaacaat aagagtcaat aatagcttat ttcgaatatc
     2881 atagaaagaa gataattcat agaagctttg cgcatagccg atcttttctc ttgtaccttt
     2941 agaatatata ggctggatag ataaaaagcc agtttttcct tctagtgtaa cgatcgttgg
     3001 aactttacgt gtagtctgaa tcaggtttcg actgttttca tgggttttaa aaatcaagtt
     3061 ctcgtccaaa tcataaacat aaagatccat tgatggctgc cctagctcag ctataaagga
     3121 atcgatcttc attaagtttc cttcgaccag tgaagtatga tctacgacct catctccgtc
     3181 atttgtcgct ttcgtcagat tacgatacgt atttactaat gtcagttctt catccgagtt
     3241 agccaaacga gaagctactt ctgaaatcgt acgctctaca ttgtttcttt cttttgccac
     3301 aatcagattg atagatgatt tataggtaat gacagcgaaa attgtaaaca caacgaatat
     3361 gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag ggccctttca gttctctttt
     3421 ttccgatttt ttcggcttta tcacgagcgc atcacataac ctgttccgcg tacagtttgg
     3481 atatagcttt cctccccggg aacgtcaatt ttgttgcgaa gataacgaat atatacgtcc
     3541 actacgtttg tttcaacttc tgtttcgtat ccccacactt tatttagtaa aacgtcacgt
     3601 gctaatacca cgttcacgtt ttccattaaa gtaagcagca actcgtattc tctttttgtt
     3661 agttcaatta cttcatttcc gcgacgtacg acacggtttt ctttttcgat agtcaaatcg
     3721 cgataagtaa tagtagtttg tttagccaca tttttatcgc cttcaatatc gattcgacgc
     3781 aacaatgcac gaagacgtgc taataattct tcgatagcaa atggtttgac gatgtagtca
     3841 tctgctccgt ggtcaagtcc agatacacga tcgatcacag aatctcttgc agtcatcata
     3901 atgattggtg tatttttgac ttggcgtaca cgtctgcaga cttctaaacc gttcaattct
     3961 gggagcatca gatccaacaa tatggcatcc cactcattat ttaatgccgc atctaaacca
     4021 gttcggccgt tgtagtggac ttctgcgtta tacccttcat gcttcaactc tagctcgaca
     4081 aatcgtgcca agttcttttc atcttcaatg attagaatat tgctcattta ttattttatc
     4141 ctttctattc tactggcttt ttaaaaactc tatcattata ccgataatat gcttaaaaat
     4201 ctatagtcat tttatcatgg atagcaattt taaaaaagag ctgaagtcta ctcataacaa
     4261 tcgacttcag ctctttcgtc ctctttttat tttctcattt cctaacagcc gaaagactat
     4321 tattcttcat ggtaccatga gtaatggaag attccttctt tgtcgacacg ctcataggta
     4381 tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg caggcagacg ttctgctctg
     4441 tatgaatcga agtaagcgat ggctgatgag aaagtcggta caggtacgcc agcttggaca
     4501 gccaaggcaa cgacgtctct gactgattgc tgatattttt tcgtgatttc aacgaagtaa
     4561 tcatctagca ataaattttc gatctcagga tttttgtcat aggcatccgt aattttttgc
     4621 aagaatcgag ctcggatgat acatccagcg cgccagattt ttgcgatttc accaaatggc
     4681 aagtcccaat caaattcttt tgaagcgaca cgtaattgag caaatccttg ggcataactc
     4741 atgattttac tgaaatacaa cgcttctcgg attttttcga ccaattcttt tttgtctccc
     4801 gtgaactcaa aatcgttcgt acgagacaaa atcttgcttg cttgtacgcg ttcttcttta
     4861 taagcagaaa tatagcgggc aaatacagat tcagtgatca atggaagtgg cacacctaag
     4921 tcaagcgcgc tttggcttgt ccatttacct gttcctttat tcccagctgc atcaaggatc
     4981 acatctacta ctggtttacc tgttccttca tcgtctttac gtgtcagaat gtcagcagtg
     5041 atttctatca aatagctgtc taattcgcct tgattccatt ctttaaagat atcagccatt
     5101 tcttctactg aaaggtttaa aatattcttc attaagtcat aagattcagc aatcagttgc
     5161 atatctccat actcaatacc gttgtgtacc attttcacat aatgtccggc tccatttggg
     5221 ccaatatatg tgacacatgg tgctccgtct tctgcttttg cagaaatttg ctctaagatt
     5281 ggtgcaacta gcgcataggc ttctttttgt ccaccaggca tgattgaagg tccttttaaa
     5341 gctccttctt caccaccgga aacgcctgta ccgataaagt tgatgccaga gttggctaac
     5401 tcttcattac gacgcatcgt atctttaaag aatgtatttc ctccgtcaat caaaatatcg
     5461 cctttatcaa ggtgaggaag taactcttgg attgttgcat ctgttgccgg tcctgctttg
     5521 accattagca agatccgacg tggtttctcg atagattcta caaattcttc gattgtatat
     5581 gttgctttga aatttttatc tgggtgttct tctactacag cggttgtttt agcccctgta
     5641 cgattgaata aagccactga atagccgcgg ctttctatgt tcaaggctag atttttaccc
     5701 ataacggcca taccgacaac gccgatttgt tgttttgaca tatgaactcc tcctacatac
     5761 gggatttctc cctaatctct tacattatag cttaacacgg ctacattgca aactaaagaa
     5821 caac
//
LOCUS       DAFEHS010000114         5702 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFEHS010000114
VERSION     DAFEHS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.120"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(113..799)
                     /locus_tag="LP327_002607"
     CDS             complement(113..799)
                     /locus_tag="LP327_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726674.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(777..1271)
                     /locus_tag="LP327_002608"
     CDS             complement(777..1271)
                     /locus_tag="LP327_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL1726675.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(1291..2511)
                     /locus_tag="LP327_002609"
     CDS             complement(1291..2511)
                     /locus_tag="LP327_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1726676.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2658..4493
                     /gene="lepA"
                     /locus_tag="LP327_002610"
     CDS             2658..4493
                     /gene="lepA"
                     /locus_tag="LP327_002610"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBL1726677.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(4587..>5702)
                     /locus_tag="LP327_002611"
     CDS             complement(4587..>5702)
                     /locus_tag="LP327_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL1726678.1"
                     /translation="FVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVLSDID
                     ETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLS
                     PALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEAR
                     PTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNG
                     TITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYI
                     DSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEIS
                     ERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         1741 a         1147 c         1085 g         1729 t
ORIGIN      
        1 acaactagtg tcagacctcc ttttttcaaa taaacagtgt tccagaagta gtttcttcga
       61 aaaataagcc gaaatttctt gaagctgccc gcttctgtca caacctcttt tattaggtat
      121 ttttcacttt ttctaattta taactttcaa agtaacgctc atctttttta ctttgagacc
      181 atgcaaacaa tcccattaag gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat
      241 ccatcaatgc tgctcgtccg acaaacagtg tgccatcaga agcagcaaaa cgttgaagat
      301 tcatgcctaa gaactggtcg cataagaatt cccagaactc ccaaaacacg ccgcaaagtc
      361 cagcaaacgc aaagccgaat aagaggaaca accaaggact ggtttttgat atctcagcat
      421 ctttcatcaa gtaaccaatc aatccatagc caagagctgt caggaccatt ggactcaccg
      481 catgtaagat tttgtcccaa aaagagatga tactgatcaa atgcatccct gtgcctaaaa
      541 agacagagat gaacaagaaa aaccagtaaa acaaaacgat tgctgctggt attttaagtt
      601 tgaaaacttt caaaatgact tgtggcatga aaatcaatgc aatccctaat aaagattcca
      661 cgataaatgt gattccttga gaagtcgaat atttttgtgt gataaattca taaaaattat
      721 agcagacaac gaatacacca aaagcaagta aagctatttg cacctttttg atttctttaa
      781 tcactgaaag tccctccatc taaaaatctt tttacttctg aaagcaactc aatttctgaa
      841 gaaaccactt ttccgttcaa tttgatcaaa cctattgtat ataagttcag gtagtgaaat
      901 tgattttcag ctgtttcttg tagtgcagcg atttttttcg ggtgggttcc gccttgctgt
      961 cgagtatcag tatataagcc gatcactggg atgcctttcg cataagccac accgatttcc
     1021 gatgctaccc cggcatcgat cgtcagccca tctagtaaag caatcattaa atcactagcc
     1081 aacacttttt ctgtgtcagc aagagcaatc attcgactgt ctgcataagc agttttgtca
     1141 ttgattgctt cattttcttg aggaagatag atcgaaagct cagaagagag ctctctgatt
     1201 tctttaacta actgttggtt atataacaaa tcacttttag caaacattgg tgcggcaaaa
     1261 taaattttca tctaaacatc atccttttta tcaaaaataa tcgctaaagt aagcttccat
     1321 gtttggaatg atacgctcta gtgacttcag tgtttcttca tctgtttcta accgttcgat
     1381 tcttgccaaa taagaagcac ggcgataatt catcatcaag caagtcaatg tttggatatc
     1441 cagtcgaacg gattttccta taggttcatt taagacacgg acttgtccct gttcatccca
     1501 tgttaaagcg aagacaccat tattccaagg agcgacagga tcttcaatga tgaaatgaaa
     1561 agcatcagct gttccaacaa acggaaaccg ttgaaggaat tcttttacat caacgatccg
     1621 agccataaaa tatggttcga tctgctcttt gatctgacta tcttcaatca aaaatgctaa
     1681 aggctcattt ttataaatat ctccatgtac ccagtagatc atggaaaaat gtgcggaaat
     1741 aaagttccat aagccatttc gagcttcttg atcaagatag aacatttctt ttacgtgaaa
     1801 tatttcttca gctacccagt agaacagaac acctcttggt tcatggttgc tatcgtagta
     1861 gacagcggct gtgcgctcat cttcattttc gaaacgccaa tactcttccc aatgaaaatc
     1921 actgcggaat aaagcaccat ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc
     1981 tggatgatca actggtaatc gctcaaccat accacttact gggatttgtt taggtagctg
     2041 tgtatctctg attttaaagg aaagtttatc agacatgatt tcccatcctt tacgccgata
     2101 gtaggggata ctgtaaggat acaaataaga gatccattgc ttattcttgc gcatattatc
     2161 caatgcaacg ataattagat cttgcatcaa tccatggtta gcatattctg gataagttcc
     2221 aactccagtc acaccaccca ttttataacg tgcaccgtga atattgactt cacaaggata
     2281 gatagcgatt tgagagatca gtttattttc gtggaaccat ccgaacactt tcgatacttc
     2341 caaaatcggt tttttggaac ggataaaagc tcgtttattc tcaaacccgc tttcctcgat
     2401 gtctgcttca gtcacttgga acacatagga aagcaattca ttgaattgat cgatatgttc
     2461 ttgagtgacc ggttttaatt caagattttc tctaaaatct ttttgatcca ttttttcatc
     2521 tccatttatt agcttgataa cagtatagca aaattctaca aaaaaaactt ctatagctga
     2581 ctaattaaaa actctttaaa ttactgcttt tcgttggtat aatggaaagt gctgattata
     2641 tgaaaagagg agacgctatg gatataaaga aaatgaaaga acgccaagag cagattcgta
     2701 atttttcgat catcgctcat atcgaccatg ggaaatctac attggccgat cgtattttag
     2761 aaatgacgca cactgtgacg tctcgcgaaa tgcaagatca gctgttagac tcgatggatt
     2821 tggaacgtga acgtggaatc acgattaaat taaatgctgt agaactcaat tacacagcga
     2881 aaaatggaga aacatatatc tttcacttga ttgacacccc agggcacgtc gacttcacct
     2941 atgaagtctc tcgaagcctt gctgcctgtg agggtgccgt tttagtggtc gatgcagcac
     3001 aaggaatcga agcacaaaca ttggccaatg tttatttagc gttagacaat gatttggaga
     3061 tcctgccagt catcaataaa atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag
     3121 aaatcgaaga cgtaatcgga atcgatgcta gtgaagctgt attagccagt gccaaatcag
     3181 ggattgggat tgaagatatt ctcgagcaga tcgtggaata tgttcctgca ccaagtggag
     3241 acttagatgc accgctaaaa gctttgatct ttgactctat ttatgatagt taccgcggtg
     3301 ttgttctaaa tgtccggatc acggacggta tggtcaaacc cggagacaaa atcaaactga
     3361 tgagtaatgg caaaacattt gatgttacag aggtcggggt cttctcgcca aaagcagttg
     3421 ctagagactt tttgatggtc ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc
     3481 aagatacacg agtaggggat acagtaacgt tagcagacaa tcctgccgct gaagctttac
     3541 caggttaccg aaaaatgaat ccgatggttt attgtggtct ttatccaatt gatacttctc
     3601 gttacaacga tttgcgggaa gctttagaaa aactgcaatt gaatgatgct gctttacaat
     3661 tcgaaccaga aacctctcaa gcattaggtt ttgggttccg ctgtggattc ctcggacttc
     3721 ttcatatgga tgtcgtccaa gaacgcttgg aacgggaatt caatctagaa ttgatcacga
     3781 ctgcaccatc cgttatttac catgttaata aaacggatgg tacgactgct acagtagata
     3841 acccggcaga ttttcctgaa cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag
     3901 cacagatcat ggtcccaaat gattttgtcg gtgcagtaat ggaactttct caacgaaaac
     3961 ggggagaatt cattacgatg gattatttag atgattaccg agtaaatgtc gtctataata
     4021 ttccgctttc tgaaattgtc tttgactttt tcgacaaatt gaaatcaagc acgaaaggct
     4081 atgcctcatt ggattatgaa atgtctggtt atcaaaaaag caagttagtg aagatggaca
     4141 tcttgctgaa tggagaaaaa gtcgatgcat taagctttat cgttcaccga gactttgcct
     4201 atgaacgtgg aaaagcaatc gttgaaaaac tgaaaaaact aatcccacgc caacaatttg
     4261 aggtgccaat ccaagcagct attggacaaa aaatcgttgc ccgttcagac atcaaagcct
     4321 tgcgtaaaaa cgtcttggct aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct
     4381 tagagaaaca aaaagaaggg aagaagcgga tgaaacaaat cggctccgtc gaagttccgc
     4441 aagaagcctt catggccgtt ctgaaaatgg acgaggacga accgaagaaa tagcaagggt
     4501 ttgtttgaaa acagtgtaaa aaataaacca tgtttacatg gatttgccac cggtttgcca
     4561 ccggtggttt ttttcggtgg caaaaatcaa gaattctttc taagattttc catgatatcg
     4621 accgtttcat ttctcatctt atctgttaca tgcgagtaag tatccatcgt aattgaaatt
     4681 ctgctgtggc ccaatcgttc agagatttct ttcatttttg cgccattttc gagaagaagt
     4741 gtagcatgag tatgtctgag agaatggaag ttaaaagaga gggagagtgc attcgatatc
     4801 ctcctagtat tccatttcac tacacttggc gtaactagct caccgtcttc cttcgtacat
     4861 actgcatttg aatcaatgta gagctttcca tacttcattc gattttctaa ttgttgtttc
     4921 ttatgttttt tcagaattgc tagcaaggtt tgtccaataa aaatcgttcg attggaactg
     4981 cttgtctttg gcgtaccata tacccatgcg ccgtcattct ttaccatttg tttctctaca
     5041 gtaattgttc catttgaaaa atcgacatca tcccacgtca gaccacaaac ttcgccaacg
     5101 cgcattcccg tataaaatcc aatattcaaa ggaatataga aaggatgtcc ttcaggagta
     5161 atttctagca tatgatcaaa gtcctcaaga gaaatgattt ttagatcttt tttagtcgtt
     5221 ggtcgtgctt catattttgg tatttttaca tacagcatag gattttgctt gattaatccc
     5281 caaggataaa ccgccatatt cagcgcattc ttaaggacag agtgagtaat agtcattgtt
     5341 ttcttcgagt aacccttttt aaattcagca ttgatgaaat tttgtaaaag agcaggggag
     5401 agatccgtaa gttttttctt tcctaaataa ccgtttatat gatttttgat ggtaaatcgg
     5461 tagttttcat aggtattgta ttttagattt agtttaacgt attcctccat ccaaaaatca
     5521 aggtattgtt ttactcgagt atccgtacct aaaaagtatt gtcctgtttc gtcaatatct
     5581 gataaaactt ttcgtaaagc agcttcggcc tctggtcggg tgtctccgcc aactttctcc
     5641 actttttttc ttgagccatc atcattgata tcttcgccgt ttgtataatt aagcgagaca
     5701 aa
//
LOCUS       DAFEHS010000115         5681 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFEHS010000115
VERSION     DAFEHS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.121"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            133..1287
                     /locus_tag="LP327_002612"
     CDS             133..1287
                     /locus_tag="LP327_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL1726679.1"
                     /translation="MEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSIN
                     GELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQ
                     REMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKA
                     GTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEY
                     RSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1385..2758
                     /gene="tet(L)"
                     /locus_tag="LP327_002613"
     CDS             1385..2758
                     /gene="tet(L)"
                     /locus_tag="LP327_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBL1726680.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3322..4584
                     /locus_tag="LP327_002614"
     CDS             3322..4584
                     /locus_tag="LP327_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL1726681.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            4829..>5681
                     /locus_tag="LP327_002615"
     CDS             4829..>5681
                     /locus_tag="LP327_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBL1726682.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1954 a          805 c         1167 g         1755 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact ttctttctta
      541 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      601 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      661 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      721 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      781 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      841 tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
      901 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
      961 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
     1021 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
     1081 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
     1141 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
     1201 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
     1261 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
     1321 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggaatt
     1381 aattatgaag tgtaatgaat gtaacagggt taaatacaac caaattttaa tttggctttg
     1441 cgttttatct ttttttagcg tattaaatga aatggttttg aacgtctcat tacctgatat
     1501 tgcaaatgat tttaataaac cacctgcgag tacaaactgg gtgaacacag cctttatgtt
     1561 aaccttttcc attggaacag ctgtatatgg aaagctatct gatcaattag gcatcaaaag
     1621 gttactccta tttggaatta taataaattg tttcgggtcg gtaattgggt ttgttggcca
     1681 ttctttcttt tccttactta ttatggctcg ttttattcaa ggggctggtg cagctgcatt
     1741 tccagcactc gtaatggttg tagttgcgcg ctatattcca aaggaaaata ggggtaaagc
     1801 atttggtctt attggatcga tagtagccat gggagaagga gtcggtccag cgattggtgg
     1861 aatgatagcc cattatattc attggtccta tcttctactc attcctatga taacaattat
     1921 cactgttccg tttcttatga aattattaaa gaaagaagta aggataaaag gtcattttga
     1981 tatcaaagga attatactaa tgtctgtagg cattgtattt tttatgttgt ttacaacatc
     2041 atatagcatt tcttttctta tcgttagcgt gctgtcattc ctgatatttg taaaacatat
     2101 caggaaagta acagatcctt ttgttgatcc cggattaggg aaaaatatac cttttatgat
     2161 tggagttctt tgtgggggaa ttatatttgg aacagtagca gggtttgtct ctatggttcc
     2221 ttatatgatg aaagatgttc accagctaag tactgccgaa atcggaagtg taattatttt
     2281 ccctggaaca atgagtgtca ttattttcgg ctacattggt gggatacttg ttgatagaag
     2341 aggtccttta tacgtgttaa acatcggagt tacatttctt tctgttagct ttttaactgc
     2401 ttcctttctt ttagaaacaa catcatggtt catgacaatt ataatcgtat ttgttttagg
     2461 tgggctttcg ttcaccaaaa cagttatatc aacaattgtt tcaagtagct tgaaacagca
     2521 ggaagctggt gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg
     2581 tattgcaatt gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat
     2641 ggaagttgat cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat
     2701 tgtcattagt tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat
     2761 cgttaaggga tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt
     2821 caaatctttt tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc
     2881 ttgtatcttt tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa
     2941 aaattttatt cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa
     3001 ttggcaaggg tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc
     3061 ccttgctgat ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg
     3121 caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt
     3181 ttggtcggca ctgccgacag cctcgcagag cacacacttt atgaatataa agtatagtgt
     3241 gttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
     3301 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
     3361 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
     3421 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
     3481 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
     3541 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
     3601 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
     3661 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
     3721 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
     3781 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
     3841 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
     3901 caaactttgg aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa
     3961 tggactgctt atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg
     4021 aaaagtgttg aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg
     4081 aaaacagaaa agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta
     4141 gtgactgctg cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact
     4201 gatatggcga gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat
     4261 agtggtcttg tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa
     4321 aacaagtctt taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa
     4381 agcactaagg aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt
     4441 aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta
     4501 gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt
     4561 cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga
     4621 tggttttgaa cttgttcttt cttatcttga tatatataga aataacgtca tttttatttt
     4681 agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct
     4741 taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac
     4801 taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga
     4861 aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa
     4921 gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga
     4981 aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag
     5041 aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag
     5101 agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc
     5161 aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt
     5221 aaataagagt ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat
     5281 taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa
     5341 ttcttataat cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac
     5401 agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga
     5461 ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat
     5521 acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga
     5581 tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag
     5641 gttaatctcc tatggtggtt tgttaaaaga aatacataaa a
//
LOCUS       DAFEHS010000116         5148 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFEHS010000116
VERSION     DAFEHS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.122"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..290)
                     /locus_tag="LP327_002616"
     CDS             complement(<1..290)
                     /locus_tag="LP327_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL1726683.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKKRDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGRKDK"
     gene            complement(492..887)
                     /locus_tag="LP327_002617"
     CDS             complement(492..887)
                     /locus_tag="LP327_002617"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBL1726684.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(897..1745)
                     /locus_tag="LP327_002618"
     CDS             complement(897..1745)
                     /locus_tag="LP327_002618"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBL1726685.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1760..2587)
                     /gene="panB"
                     /locus_tag="LP327_002619"
     CDS             complement(1760..2587)
                     /gene="panB"
                     /locus_tag="LP327_002619"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBL1726686.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2599..3459)
                     /locus_tag="LP327_002620"
     CDS             complement(2599..3459)
                     /locus_tag="LP327_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBL1726687.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3697..4866
                     /locus_tag="LP327_002621"
     CDS             3697..4866
                     /locus_tag="LP327_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL1726688.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT         1635 a          966 c          653 g         1894 t
ORIGIN      
        1 tacttgtctt ttcgtccgaa tatcagcgcc gcttgcttta aaatatcatt ctccatttcg
       61 agttgcttat ttttctttct tagtgcgatc aattctgctt gttctggtgt taagttgtct
      121 ctttttttga atgagcccgt tgattgtgct tgcttcatcc atttatcgaa agaagaaggc
      181 gtaagctcat actctcgaat aatttctgcg cgaggcttac cagcgagata gagattgacg
      241 atttgttgtt tgaattcttt tgaataggtt cttcgttgac gtcttgacat gaaaaatcct
      301 ccagtgtgtt ttttattatt ctacacacct tattttttct gtctagtcta gtgtagccga
      361 ttcacataaa atggactttg tctacagtct gtatcttctc tttttgtgtc tactttattg
      421 acatctatcc aattagtaat caataaatat ttcaataata gtctagctat atataaataa
      481 cattacaaaa cctaaagaat tatatctgat aaccttccat gtttttcata tgatgttact
      541 ctttcaactt tattattatc atcgacaaaa acaaccttag gtctatgatc cttaacctca
      601 ttttcattta attcacaata tgccattaaa attattttat ctccagttga tacacatcta
      661 gctgcagctc cattcaagca aatcatccct gttcctctct ctccagctat tgtataagtt
      721 tcaaatctat ttccattatt aacatcaact atttgtactt tttcatattc atatattcct
      781 gctgcttcta ataaattcat atcaacagta atacttccta cataatctaa ctcagcttgt
      841 tcaactgtag ctctatgtat ttttcctttt aacattgtaa ctttcattat agccccctat
      901 tgtaattcaa atgtaaaatt atctattaat cttgtttttc ctataaatac tgctattgca
      961 actaaaactg atttttcaat ataatttact ctttgtaatg ataaagaatc aacaacttct
     1021 acataatcaa tctttgccaa attataagta tttatatttt tacttattat ctcaattatt
     1081 tttaagctgt ctctctctcc attatttaaa gcttccttag ctaaagttaa agatttattt
     1141 aaaatagtag ctgaagatct ttcctctaaa cttaagtatg tgtttctaga acttttagct
     1201 agtccatcct cttcccttat aattggacac cctacaacgt ctatatcaat attaaggtcc
     1261 ctaaccattc ttttaattac agctaactgt tgtgcatcct tttctccaaa ataagctcta
     1321 tctgctggta ttatgttaaa taatttactt acaacagtgc aaacccctct aaaatgtatt
     1381 ggcctactag ctccacataa accttctgtt aatccagtta tattaacaaa agtacttgca
     1441 ttgtcacaat acatctcttc aacttcagga ttaaatattg ctgttgctct acctcttgtg
     1501 catacttcta tatctctttc taagtctctt ggataactac ctaaatcttc atttttccca
     1561 aactgagttg gatttacaaa gatacttaca acaactttat cattttcttt tgatgctttt
     1621 ctaattaaac tttcatgacc ttcatgtaaa tatcccatag taggaactaa tcctaccttt
     1681 aagccttgtt ttttccactc ttttacttga cttctaacat cattcactgt tttaaatata
     1741 accatatttc ttgacccttt taatataatt tttctaaaac atcatcactt attttaaacc
     1801 catgttctgg acctggaaaa actccatctt ttacttcctc tatatatttt gtaaatgctt
     1861 tattcatctc ttctgataaa ttagcatatt tcttcacaaa tttaggggta aaatcactat
     1921 acatccccaa catatcttga tatacaagaa tttgtccatc acaccctgca cctgctccaa
     1981 taccaattgt aggtattgat atctctttag aaattaactc tgcaagctta gttggaacac
     2041 attctaacac tacagcgaat gctcctgctt tttctacagc tttagcagct cttattaatt
     2101 ctttagcagc ttccttgtcc tttccttgta ccttaaatcc tccaaatgca ttaacagatt
     2161 gaggtgttaa acctatatgc gccataacag gtatagaagc ctttactata gcctctattt
     2221 tatcgcatac ttctatccct ccttctaatt taacaacctg agctcgtcct tcttttatta
     2281 atcttcctgc attaactact gaatcatata ctgaagtttg ataagacata aatggcatat
     2341 ctgcaacaac taaagtgttc ttagctcctc ttgtaactgc tctagtatga tgaatcatgt
     2401 cttccattgt tactgaaagt gtatcttcat gtcctagaca taccatacca agagaatctc
     2461 ctactaatat tccatttatc ccagcttcat caattatttt tgctgttgaa tagtcataag
     2521 ctgtaagcat tgttaatttt tcatttctta atttagattc tttaaatgta actgctgtat
     2581 ttttcatcta tttctctcct aactctctcc taaatacttt tctaattcac tataatctct
     2641 attaatattt tttaacatag ctatctctaa tacatttttt gataatagca tatataaagc
     2701 tctgtcttca tctgataaaa cttcacagtg acctttaatt gttgaaatat cacaacgttc
     2761 aactggccct gtcaaacttt ctataattcc tttttctttt atatttctaa tattaaattc
     2821 aattaatgga tataaagctt caattgccat tttttcagta aatccacatt gaattagata
     2881 tgtaacgctt gtattaataa gtcctagcac aaggttacta gatactactg atgcggcatg
     2941 ataaagagat ttattctcct tagatacaat tacagtacta ttacctaact ttttgaagaa
     3001 atcacataaa aactttgcat atttttcatc gccttcaatt gttataaaag atttttttaa
     3061 ttttttgtat gaattatatt tatctgatat tgaaaacata ggatggacag aatagccata
     3121 agcaccatag tcctgtatat ttgaaaagat atctgaagat attgcaccac tgcaatggca
     3181 gattaatttg tttttgattt gataattttt aatttcttgc catacctcct gaatttgacc
     3241 atcaggagtc gtaatgaaaa tagtatcatt ttcatctact aattctctta agtttaaata
     3301 ttgtttacta gatgtaaaat tcgaagctgc taatgacgag tcttcgcttt tactataaaa
     3361 tcctgttact tgtattttat gttcctggaa atacttcccg aatgaacaac ctaccttgcc
     3421 ggctccaata aaaccaacgt tcataattat cacctccata aagaagtgag actacaagtc
     3481 ccattcttac gatatgagct taaaaataaa aattaaatca aatatacagt atgttttttt
     3541 caaatatcat ccatagttaa tttatcataa tatcccatat tttaaaataa aagatagcgt
     3601 aaaatatttt gtgtaaatag aaatttaggg tagaaaaaaa agtccattct gtaaaattaa
     3661 agttaccaca cagaaatttt ataggaggac tttctcatga ctaaatttac tatagaaatt
     3721 atgcaaacac tactcaataa aggggattta gatgaattat ttcaggatca tttagagcga
     3781 gcgattaatt cacttctaca agcagagcta actgcttttt tagattacga aaaatatgat
     3841 cgaaatggat tcaattcagg aaattcccgt aacgggaatt attcacgtac atttaaaaca
     3901 gaatatggcg agttgaattt aactattcct agagatcgga atggcgaatt ttctcaacaa
     3961 acactaccag catataagcg aaccaatgat tcattagaaa cgaccattat ccaattattt
     4021 caaaaaggca ttactatggc tgaaatctcc aaattaattg aaaaaatgta tggtcaccac
     4081 tatacgccac aaacaatctc caacatgagt aaattggtgg ctgaagatgt tttagctttt
     4141 aaagaaagaa cgttagaagc caattattcc gttatattta tggatgcgac gcatatccct
     4201 gtgaagcgac agactgtttc gaaggaagca gtgtatatta cgattggtat tcgtttggat
     4261 ggaaccaaag aagttcttgg gtttactatt gccccaactg agtctgctta tatttggaaa
     4321 gaagttcttc aagatcttag aaaacgtggg ttagaagaag ttttattagt agtgacagac
     4381 ggattaagct gtattgaaga aagtatccat agtgtgtatc cgaatgccca atttcaacaa
     4441 tgttgtgtgc atgtatctag aaatatcgct cataaagttc gtgttcgagg tcgaaaagaa
     4501 atttgtgagg atttcaaatt ggtttaccaa gcgaattcaa aagaagaggc attggatcac
     4561 atcgacttta tgactaggaa atggaaaaag cagtatccaa gagtcgtcaa tttactcttg
     4621 aatcctgccc tattaacctt ttataatttc cctcacgcca tcagacgaac aatttattcg
     4681 acgaacctga ttgaaggctt taataagcag ctaaaacgat atactcgaag aaaagaacaa
     4741 ttccctaatg aagaatctct agagagattc ttcatttctc aatttaatca atataaccaa
     4801 aaatttttag gtagaattca caagggattt aaagaaattc aggatacatt agagtcgatg
     4861 atttaactgt aattaacaga atggattttc catttacata taattcttga cgctaccata
     4921 atctaaataa ccatcatatt catctactac ttatataatt gagttttatt aaacttttta
     4981 agtttctatt ctatcaaata tttaaacaat ctctgcatcc gaaaagacta cctcgtatag
     5041 ttttactaac aatattttta ttagcgtgtt caaatcctga tttcttgtat caattatttt
     5101 ctgttcactt tcaataagaa aaaagtcttt acttccatgg ataataat
//
LOCUS       DAFEHS010000117         5147 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFEHS010000117
VERSION     DAFEHS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.123"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            127..579
                     /locus_tag="LP327_002622"
     CDS             127..579
                     /locus_tag="LP327_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726689.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="LP327_002623"
     CDS             593..1282
                     /locus_tag="LP327_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726690.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="LP327_002624"
     CDS             1407..3758
                     /locus_tag="LP327_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBL1726691.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="LP327_002625"
     CDS             3838..4233
                     /locus_tag="LP327_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726692.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..>5147
                     /locus_tag="LP327_002626"
     CDS             4558..>5147
                     /locus_tag="LP327_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL1726693.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKE"
BASE COUNT         1813 a          670 c         1035 g         1629 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaata
//
LOCUS       DAFEHS010000118         5041 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFEHS010000118
VERSION     DAFEHS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.124"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(12..473)
                     /gene="mscL"
                     /locus_tag="LP327_002627"
     CDS             complement(12..473)
                     /gene="mscL"
                     /locus_tag="LP327_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBL1726694.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            725..1993
                     /locus_tag="LP327_002628"
     CDS             725..1993
                     /locus_tag="LP327_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL1726695.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            1986..3272
                     /locus_tag="LP327_002629"
     CDS             1986..3272
                     /locus_tag="LP327_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL1726696.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            4011..4934
                     /locus_tag="LP327_002630"
     CDS             4011..4934
                     /locus_tag="LP327_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726697.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         1695 a          914 c         1043 g         1389 t
ORIGIN      
        1 tagattcttt attatttaga agtatcggaa agatttgttt gctctactgg atgtgtattg
       61 tcttctgcca cttgtttggc aagaagatcg cgtatgtcac taagataatc ttctgcagta
      121 ggagtttctt ccacttccac ttgctcttct ttggagcgta aattcttcgc acggtttgcc
      181 gctttgacaa ctaagaataa gacaaaacca gtgatcagaa atgtaatgat agcactaata
      241 acgttaccaa acgcaaatgt gacgcctttt gtcggcgacc agtcaagaat tgaaagtgct
      301 ccttctaaat cagaagtacc tgtgatcatt gcgacaaacc aacctattag tggtgtgatc
      361 aagccttgca ccacttggtt tacgatgctt gtaaacgcac taccaataac aacgccgaca
      421 gcaagatcca agacatctcc acgcatgatg aactctttga attctttgat catgaatgac
      481 cctcccttct tttatttgtc tttagtatat cgaaaaataa aaaataaaaa aacaatatac
      541 gcttttagcc gtctgtttct tttgataaaa aacatctatg ctatactata aagtgtaaca
      601 tcatttagaa aaaacggcga attgcctgaa cttataaatt tcaagcagag gaataagtaa
      661 aaaaggcagg ttgacccatt aaaagagtaa gcgggcaatc aactgtgaaa aggagcatat
      721 aaatatggtg tatcaattgg cagaaggcgt gaatcttcat gtcttaccaa caaaacaata
      781 caaaacgatc cggattttta tccgttttac tgcgcgtctg caacaagaag tcatcacgaa
      841 acgttcactt ctcagcagca tgttggaaac aaatagtttg aattatccgg atcagacaaa
      901 gttaagtgcg caacttgcag agctttatgg cgcaagcttt ggattatcag ttagaaaaaa
      961 aggaaattta cattggctaa acgcagggat cagttttgtg aatggtgaat atgtcaatga
     1021 tccaaacctt ttttctcaag cggtagattt tttaaaagag gttttatttt atccaaatat
     1081 taaaaatcat cagtttgatc agttgacgtt tgacttggaa aagaacaatc tgcgtctata
     1141 tttggaaagt ttgaaggaag ataaacaaac ttttgcttcc tatgccttgc aagaacttta
     1201 ttttgaaaac agcccggaac aaaagatccc agggttgggc gtagtcgaag atttggacaa
     1261 gatcactgct agttcgctag cgtcctacta tcaagaaatg atggcaaatg atcaaatcga
     1321 catttttgtt gtcggcgatg ttgatccgga caaagcagct gaagcagttg ggcaattacc
     1381 atttgagtcg cgggaaacag tttatccaga tctgttttat acccaacctc aagtgaatat
     1441 cgtgaaggaa cgacaagtac gtgagccaat cgttcaagca aagctgaacc tagcttatca
     1501 gacaaatgtg tattacgatg aaccagaacg ttttgcgcta atggtcttta acggtttatt
     1561 tggcggtttt cctcactcta aacttttcat gaatgtcaga gagaaagaaa gtctagctta
     1621 ttatgcctct agtagcgtgg atacgttccg aggattcatg agtgtccaaa ccggtatcga
     1681 tgaaaagaac cgaaatcaag tacttcatct gattcacgag caattagaaa gtttgcgaaa
     1741 tggtgaaata acagagttgg aactgtctca gacaaaagca atgctgagaa accaatatct
     1801 gctttctcta gactctccgc aagcggcaat cgaagcttct tttttggaca gctggctgcc
     1861 ggaaacaaaa ttatctgatg aagaatggtt gaaacgtatg gagagcgtaa cgatcaaaga
     1921 gatacaacaa gtagctgagc aaatcgaatt gcaggcaatc ttctttttag caggaggaaa
     1981 tgcaaatgaa taaaacagaa tatgagcaaa tcaatgaaac actttatcat gaagtactgc
     2041 caaacggttt gaccgtgtat ttgttgccta aaaatgacta tcacaagaca tatggtttgt
     2101 tcagtacgaa ttatggttcg atcgataacg aatttattcc ctatggtgaa aaagaaaagg
     2161 tgaaagttcc agatggaatt gcccattttt tagaacataa gttgtttgaa aaagaagatg
     2221 gtgatgtctt tcaattattt gggaaacaag gagcttccgc caatgctttc acaagtttta
     2281 cgaaaaccag ctatctgttt tctacgacgg atcaggtaga aaagaatctg acgactctga
     2341 tcgattttgt acaagcacct tacttcacag aagaaactgt aaacaaggaa aaaggaatca
     2401 tcggtcagga aatccaaatg tatgaagacg accctaactg gcggatgttt tttggcatac
     2461 taaataactt gtatcctaca cacccattgc atatcgatat tgcgggcacg gttgaaagta
     2521 tcgacaaaat cacagctcaa gacttgtata cgtgctatcg gacgttttat cagccaagca
     2581 atatggtttt atttgtagtt ggtaagatgg agcctgagaa attgatgaaa cttattcggg
     2641 aaaaccaaga agcaaaaaat ttccctccaa agcaagaaat tgtacgttat ttccctgaaa
     2701 acacaaaaga aatcatcaaa caaagtgcat tggaagcagc cattacacga gataaatttg
     2761 tacttggtat taaaggatta gacacattgc cgcaagaagg aacagaactg ttacgctata
     2821 aaacagcaat caatctgttg ttccaaatga ttttaggcaa tacttctcga aactatctag
     2881 cgatgtacaa tcaaggaatt atcgatgaca gctttggttt tgaattcagc ttggatcgtg
     2941 aatttcactt tgcggacttc agcggagata cggacgaacc agagaaagca gcagaaaaag
     3001 tcaaagaaat cattcttgga ttcgctgatg atccggaagt gtcagaaaca aatctcgatt
     3061 tgctgaagaa gaaaatgctt ggacaatatt tccaatcatt aaattcgatc gaatatattg
     3121 ccaatcaatt tacgcaaagt ttatttggcg atcgaacttt atttgatctt cctgaaatca
     3181 tcgattcgat ccaaatgaaa gacgtactgg cagctgggga agcatttata agtgaagagg
     3241 ccttcagcga gttttacatg agaccaaaat aaaccagtat tttatgagga aaagtcttaa
     3301 atttcgtttt agacttgtag taggaacctc ctccaaaatc agcttttatg gtattcttag
     3361 ttgtgttgga aaaattagtg agaaaataaa acaaatcatg ctctctttta atcaacttat
     3421 attaaaataa aatcaaacga agtaatcaaa gaggttgtga cagaagtggt cagcttcaag
     3481 aaataagaag gaatttacga aaattgcttc tcaaattttt gtgatatttc ggcttattgc
     3541 cgaagaagct acttctggaa caccgtgtac caagaggttg tgaaaaagct gccctgcatc
     3601 aagtaataag aacagccaag taaaaacagc ttcttaatgt ttttaacttg gttggactta
     3661 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcgtttt
     3721 gacctgagta ttagagaaac atacggaaaa atatctcctt atattttgcc ggtttttgag
     3781 ctaataccga ggaacagctg attgaacacc gtttaagaaa gaggtcgtga gcctgccgtt
     3841 taactccgag tcacaaggaa caagttcaaa aaacagcttc ttatatttat tggatattgt
     3901 gacttgtgac cgaggagttg gcaggagaac aatgtttatt cggaaaaaag gagctgtgac
     3961 atgacttttg tcacagcacc caaacataac gtctttcgtt gcaacagcag atgcttcgga
     4021 gaatcaaaca gactggagaa gatgaacgag tggcttttga aaccatcgga gaaaaattac
     4081 ggcaagcacg cctgaataaa aaaatatctt tagatgaatt acagcagatt acaaaaattc
     4141 aaaaacgtta tttggaagca atcgacaatg atgattttga ccaattgcct ggtaaatttt
     4201 acgtgcgggc atttattcgg caatacgctg aagctgtagg agaagatgga gaccatttag
     4261 tcgatgtttt tgatgggaaa agcaaattga caggatcggt ccctgaacgt ccgcagcctg
     4321 aaacagtaag tacttctaga aaagcaatgc atcaggaaga atcgcatcca agtcgctttt
     4381 ggacaagtct tcctgtcatt ttattaggat tggttgcttt ggcgattgtc gttgtcgtgg
     4441 gctatatgac ttggcaagat cgtcaagctg atccaataat cggagacaca gcttcttcgc
     4501 ttaaagtaga aggatcagtc gaggagtcct cttcctcaga gcaaagcacg cagaccaaag
     4561 agtcttctac agtagaatca acaacaaaat caagtgaaga aaagaaaatg gcaatagcta
     4621 caggagaaga ttctggcagt gccatcgcag taaatatcac agacgctacc aagcctgtaa
     4681 cccttgaatt tactgctgtc aatcgtgtat ggttaggtgt gctagtagac aatgcctatg
     4741 tttatcaagg gacactggca gcaaatgaaa cacagagtac cgtgttaccg gaaacagcaa
     4801 caaatgcgac catcacgatt ggtgcagcaa gcaatgcaac gatcaaagcg aatggagaat
     4861 ccgtaccagt taatccaggg gaaaacaatc agagtccaaa aaatgtgaac cttactcttc
     4921 aatacgcaga atgatcgtat aaattagttt acgcatctgt ttacttgtag gtataaaaaa
     4981 gaaggatcgt gacaaatctg tgtcgcggtc cttcttcgtt aattcaaaaa gatcaatttc
     5041 a
//
LOCUS       DAFEHS010000119         5021 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFEHS010000119
VERSION     DAFEHS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.125"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            282..521
                     /locus_tag="LP327_002631"
     CDS             282..521
                     /locus_tag="LP327_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726698.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="LP327_002632"
     CDS             complement(883..1143)
                     /locus_tag="LP327_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726699.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="LP327_002633"
     CDS             complement(1328..1825)
                     /locus_tag="LP327_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBL1726700.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="LP327_002634"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="LP327_002634"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBL1726701.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="LP327_002635"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="LP327_002635"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBL1726702.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQVELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5014)
                     /locus_tag="LP327_002636"
     CDS             complement(4547..5014)
                     /locus_tag="LP327_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726703.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1528 a         1041 c          770 g         1682 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctactt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat g
//
LOCUS       DAFEHS010000120         4954 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFEHS010000120
VERSION     DAFEHS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.126"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(207..1169)
                     /locus_tag="LP327_002637"
     CDS             complement(207..1169)
                     /locus_tag="LP327_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL1726704.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="LP327_002638"
     CDS             complement(1171..2610)
                     /locus_tag="LP327_002638"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBL1726705.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="LP327_002639"
     CDS             2795..4741
                     /locus_tag="LP327_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBL1726706.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1605 a          830 c          804 g         1715 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactcgat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gata
//
LOCUS       DAFEHS010000121         4807 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFEHS010000121
VERSION     DAFEHS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.127"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="LP327_002640"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="LP327_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBL1726707.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="LP327_002641"
     CDS             complement(3250..3768)
                     /locus_tag="LP327_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBL1726708.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          785 c          730 g         1713 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taatgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DAFEHS010000122         4663 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFEHS010000122
VERSION     DAFEHS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.128"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            204..548
                     /locus_tag="LP327_002642"
     CDS             204..548
                     /locus_tag="LP327_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBL1726709.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            549..968
                     /locus_tag="LP327_002643"
     CDS             549..968
                     /locus_tag="LP327_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBL1726710.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1060..1410)
                     /locus_tag="LP327_002644"
     CDS             complement(1060..1410)
                     /locus_tag="LP327_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726711.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1376..2707)
                     /locus_tag="LP327_002645"
     CDS             complement(1376..2707)
                     /locus_tag="LP327_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBL1726712.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2963..3529
                     /locus_tag="LP327_002646"
     CDS             2963..3529
                     /locus_tag="LP327_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL1726713.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3674..3761
                     /locus_tag="LP327_002647"
     tRNA            3674..3761
                     /locus_tag="LP327_002647"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3706..3708,aa:Ser,seq:cga)
     gene            4095..>4663
                     /locus_tag="LP327_002648"
     CDS             4095..>4663
                     /locus_tag="LP327_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBL1726714.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1691 a          784 c          896 g         1292 t
ORIGIN      
        1 tttgtctcgc ttaattatac aaacggcgta aaataaaaaa agcgttagca actatgctta
       61 aaaagcatat cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg
      121 aaatgcgaag aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca
      181 gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag
      241 gagattttgc accaaaattt gcagaattga atgatgatgt tttatttgga caagtatggt
      301 ccagagaaga attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag
      361 gaggaaattt tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta
      421 gtgaagaaat agcagaaatc attacccatt tagcttttta tgtaggatgg ccaaaagctt
      481 ggtcagcttt tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag
      541 aggcgtaaat gacgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg
      601 aaaaaaacga agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga
      661 atgatcccga agtttctgtt ggtgtaggta atgtaatttt tgaaccaggt tgtcgcaatc
      721 actggcatgt ccataaagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt
      781 atcaggaaga aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca
      841 aagggattaa acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca
      901 ctgctggtaa ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa
      961 atcaataatg tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc
     1021 tgcttttttg attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc
     1081 acgtcgaaag atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat
     1141 caaactacct aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg
     1201 atagtagatt tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa
     1261 atcttctggc tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa
     1321 ttctactcca atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc
     1381 gatgattctt tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa
     1441 agttatctag tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat
     1501 atagatttag tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagtacac
     1561 cagctattac accgtaaaaa ctgccgtaaa aaccgcttat gggcgcgaga cctgtcccaa
     1621 acacagctgc aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag
     1681 caataaatat tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga
     1741 aacccaccgc agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca
     1801 aaacataaga agacagcatc agaccaacta agcctatgtt gatcaaagta gcaccaatac
     1861 cagaattagc aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc
     1921 ccaaaagccg aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa
     1981 aaataatcca aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg
     2041 aatagctgaa taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc
     2101 caatattata aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa
     2161 gaagtagacc aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa
     2221 aacgcatacc aaaagtgata taactaacga ccggtgataa tgcgctacca aacagtccga
     2281 tcatgatgta ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa
     2341 tgattgagat agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa
     2401 tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa
     2461 gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa accgcctaaa gcaaaataat
     2521 ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag
     2581 gagtattaga aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat
     2641 actttctttt ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt
     2701 tttccattac acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg
     2761 acaactatca tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt
     2821 tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa aaatttaaaa tataacgttt
     2881 tgagtaaaat gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca
     2941 gtaaagaagt gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa
     3001 agctgcttat gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc
     3061 taaaaagatg gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt
     3121 aaaaaatacg ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga
     3181 acgtactggt gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca
     3241 tcatagtctt tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga
     3301 tttttcatac aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc
     3361 ggatgaatat gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc
     3421 attgatgtca tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg
     3481 gacaattgac tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag
     3541 tatgaatttg atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa
     3601 aagcgatttc tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa
     3661 aaatagtact tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac
     3721 cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt
     3781 tgtgaagaag ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta
     3841 ttttttcgtt tgattgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
     3901 aataaggggc tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca
     3961 atattttttg attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt
     4021 ggcctttttt tgataaaaaa aatactagat gtaagataaa agtgtaaaaa cgatttgttt
     4081 taggagggga aacgatgtgt accgcaatca cttatgcaac gaaagaccat tattttggaa
     4141 ggaattttga ttatgagatg tcttataatg aagtggtcac tattactcca agaaattatc
     4201 gtttcgattt tagaaaggta aaaaatttag ataaacatta tgcaatgata ggaatagcag
     4261 ctggggtatc caactatcca ctctattatg aggctacaaa tgaaaaaggg ttgagtatgg
     4321 caggactgaa ttttcctggc aatgctgatt ataaagaatt acaagaagga aaagataacg
     4381 ttgcaccatt tgagtttatt ccatggatac tagggcaatg ctcaaccata gacgaagcta
     4441 aagaattatt ggctactatc aatctagtca atatcgattt tagtgaaaaa ctacccttgt
     4501 cacctttaca ctggttatta gctgataaag aaaaatcgat agtaattgaa agtatgaaag
     4561 atggacttca tttatatgat aatcctgtcg gtgtgttgac aaataatccg ccatttgatt
     4621 atcaattatt taatctgaac aattatcgct cgctatcaaa tgg
//
LOCUS       DAFEHS010000123         4619 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFEHS010000123
VERSION     DAFEHS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.129"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(16..963)
                     /locus_tag="LP327_002649"
     CDS             complement(16..963)
                     /locus_tag="LP327_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBL1726715.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1141..1944)
                     /locus_tag="LP327_002650"
     CDS             complement(1141..1944)
                     /locus_tag="LP327_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBL1726716.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2090..3004
                     /locus_tag="LP327_002651"
     CDS             2090..3004
                     /locus_tag="LP327_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL1726717.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3052..3522
                     /locus_tag="LP327_002652"
     CDS             3052..3522
                     /locus_tag="LP327_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBL1726718.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3607..4215
                     /locus_tag="LP327_002653"
     CDS             3607..4215
                     /locus_tag="LP327_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBL1726719.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4349..4549
                     /locus_tag="LP327_002654"
     CDS             4349..4549
                     /locus_tag="LP327_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL1726720.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1512 a          716 c          912 g         1479 t
ORIGIN      
        1 tattgttttc agctattaat atcctgcaga caattttact tgattggttt gaagctcatg
       61 ggtggcaaag tagcttttca gattggttaa aaaaatagcc attaatttct tttgaaaatc
      121 aggtgtcaaa cctgcaatgt gggaagtaat cagtacattg tccattttcc aaagaggatg
      181 atcttgtgga agaggctctt cctcaaaaac gtctaatgct gcaaaggaaa gttgctggtt
      241 atttaaagcc tgcaccagat cttgtgtctt aacagaagca ccacggccaa cattgatgaa
      301 tacagatttt ggatcaaagt gttcaaacat atgctgatca aagagcccag ttgtttcctc
      361 cgttagaggt aagatattga ctacgaaatc tacttcagga agaacttcct ttagttgatt
      421 taaagaaaaa gtcttttcca ttccttctgc tggatgaccg gatgtattga ttccatagca
      481 gttcatgtct aatgctttgc atttttttgc aatcattttt ccaatatggc cagttccaat
      541 gattagtatt tttttccctt ggatagattg gtaatggacc gatgaaccta accatttcat
      601 gtcactctgt gccttttgtg cttgaaaaag accacgagaa tacccaagga tgattcctaa
      661 gacatgctcc gtaatcgatt cactgtggat gccactacca tttgaaagta aaatatgttt
      721 ctttgaaaaa gtctcaaggg gtagctgatc aacaccagct gagatagatt ggacccattt
      781 caaggtagaa gaggctaata gtttttcttg gtagtctgag ttccagccca aactgatctc
      841 tacattttgt agatcctctg gttgtaattc tgttttaaag atataaccag gcgctatagt
      901 atggatttct tcgataaatt catcacgaaa tggacgatct gcataaatga ttctttctga
      961 catagttaat ttcccgcctt tctttactct aagtgtagcg catacaagaa gaactttgaa
     1021 gcgccttgct tgttttttga gaaagaaata tttttcaaat aaaaaagggg ctgtgacaaa
     1081 aaaatgtcac aacctctaaa agggtctatt gaagtagaaa taacgagttg tctttatttc
     1141 ttaattgatc gatatttttg cccatccttt gacagcttga gcaaaaataa agattcctgc
     1201 aaaagcaatg aaggcgctag tgtcaaaaga aggaagaaag accatcaaga ttgttgcaat
     1261 tatagtaggc aatgaaattg aatagatcaa tgtttttaaa caatcaagaa aagtagcttt
     1321 tgttagtcga aagcgtgcat agagaaaagc agcaatgttg gtaaatagca aagtgatcag
     1381 cagattcaga aaactaggat aaatagaaaa tataaatgtc aggactttta tccaaagtgg
     1441 tatagatgct tcacttaaaa aagaacggag ctgcttacct gttaagtttt ttaaagattc
     1501 attggtgtag ggaagttcta attggttatt gttcaacaaa gcagaagtcg tgcctgtatt
     1561 aggaagagct atgataagtt tgttttttaa catcccgaca ctaacaaaat tccccatcaa
     1621 atcagtagag atgtcctgtt ctgttcgttt tccctctgga tcaaatgtga agataatgga
     1681 gtttgtttgg taaatgaatc cttctttttc ttcggtttga agctgcccgt tttggattgt
     1741 aaaatcagga atgcgagtag caatcttttg cccatccaat tgaatgttgt ttaagacttg
     1801 aaaaacttga aaagagattg gcaaagccat aattatactc aaaactaaaa gatagacaat
     1861 ggctttccaa aatgggacat ttctcgcctc ctttaggtct ttgaatcgta taaaggaact
     1921 gataattaat tgttttgttg tcataagtac tcctcatcta gtagtgtcat cacttgcaat
     1981 tctagcgaag tatcaagtga aatacaagtg ctttaaggaa acagctgaat gattggtgaa
     2041 atatcaaaaa aatgttattc ttgtctaaag actatccaat ataaataaaa tggaggaagg
     2101 aatagtgcct acacagccac agagatcatc aaaaaaacta tttggagtag tggttttgaa
     2161 tgcggtgatc actttatcag aatttattgg agggattatg tcagggagtt tgtctcttgt
     2221 ttcagatgct ttccataacc ttagtgatac attggcaatt attttttctt acggggcaca
     2281 aaaaatggcc cgaaaagaag caaataaaaa acgtacgtat ggctaccagc gtttagaaat
     2341 attgagtgct ttcatcaact cattcatatt gatcatatta tccttgtttt taaccgttga
     2401 agcattcaag cggttcactt caccagaaaa gataaatagt catttgatgc taacagtagc
     2461 agttattgga ttgctcgcga atttgttctc agtattatta ttacgacaag aagcagatga
     2521 aagtttgaat ataaaatcta gttatttgca tttgttaagt gatacactgt cttcggtaag
     2581 tgtgattatc ggagcaattt tgatccgttt tttcgatatc tactggatcg atccagttat
     2641 tacactgatc atttcgatat atatattgat agaagcgata atggtgatca aaaaagctgc
     2701 cgctattttg atacaaagtg cacctgctat cgactatgaa aaaatggaac aagaaattga
     2761 agcaatcgat ggggtaaagg atgttcatca tgtccatatt tggcagtata gcgaaaagat
     2821 aattattttc gacggacata ttgattttga agatcagcta ctaagcgaga ttgaaaagat
     2881 atatcctcgt ataatttctt tattaaaatt aaaatatggt attacacatg tgacgatcca
     2941 agcagagaca aaggcaaagg atcaaaaaaa attaattttt ttaaataaag atgaaagtta
     3001 ttgaaatata gagtatgata aataagaatt tttaataagg taggttggct catgaagcag
     3061 tatcgacagt taaaaggata tttagaagca aaaggatatt gggagatact agtctatctc
     3121 ttttttggtg gtttggctac gattgtaaat tttgttagtt ttgctctcgc acgccaatat
     3181 tttgatttaa gtatggcctt atccaattct atttcgtggt tttgttctgt attgtttgct
     3241 tttgtaacga acaaactttg ggtattccat tcaaaatcac cgaattttac acatgcactt
     3301 attgaatgcg gtaagttctt cttctaccgt atcctttctt acggattgga catgggggca
     3361 atggtattgt tgatcaatgt tatgaacagc aatgagtatg tggcgaaaat catcactcaa
     3421 attattgtta tccttgcgaa ctatattttc agcaagttgt ttatttttaa agaaactgag
     3481 gtttttgaag aagaagtagg tcaaagtgaa aaagacgact aggcataaca attgaagaat
     3541 ttccttctct ttgttactct ttgtaatgta gacattatta agaaaacatt gaggaggaat
     3601 ttttttatga cttacacatt accagatcta ccttatgcat atgacgcatt ggaaccttat
     3661 attgacgaag aaacgatgca tctgcatcat gataagcatc acaatactta tgtgacgaat
     3721 ttaaattcag caattgagaa atacccagaa ttaggcgaaa aaacaataga agaattatta
     3781 tctgatatgg acgctattcc aacagatatc aagacagctg tacgtaacaa tggtggcgga
     3841 catgctaacc attcattttt ctgggaaatc atggcaccaa atgctggtgg cgaacctaca
     3901 ggagaaataa aagaagcgat taatgaagct tttggtgatt tttcttcttt taaagaagaa
     3961 ttcaaaaaag cagccgctgg acgatttggt tctggatggg cttggcttgt aatggaaaat
     4021 ggaaaattag ctattacctc tactgcaaat caagattctc cattgatgga aggaaagaca
     4081 ccaattctag gtttggacgt ttgggaacat gcttattatt tgaaatataa aaatgtccga
     4141 ccagattata ttgcagcatt ctggaatgtt attaattggg atgaagtaaa caaacgtttc
     4201 gaagcagcaa aataaccatt tcctccgtca tatcagtctt aaaaataatt tggaaaataa
     4261 aagagcgaaa gcttgctagc gttttctaaa ttgggtataa tgttcttgca aggttacttg
     4321 caagaacttt tttggaggat atacatttat ggaacacgga acggttaaat ggttcaacaa
     4381 tgaaaaagga tttgggttta ttacagtaga cggcggagat gacgtatttg ttcatttctc
     4441 agcaattcaa ggagacggct tcaagagcct agaagaaggt caagaagtag aattcacgat
     4501 tgttgaaggt gcacgcggac ctcaagctgc tgaagtgact aaattataat aatttgttga
     4561 ataaagacac gacttcttag agagtcgtgt ctttttgtct atttatgaag tttttagat
//
LOCUS       DAFEHS010000124         4487 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFEHS010000124
VERSION     DAFEHS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.130"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(177..680)
                     /locus_tag="LP327_002655"
     CDS             complement(177..680)
                     /locus_tag="LP327_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL1726721.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(803..1567)
                     /locus_tag="LP327_002656"
     CDS             complement(803..1567)
                     /locus_tag="LP327_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBL1726722.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1675..1950
                     /locus_tag="LP327_002657"
     CDS             1675..1950
                     /locus_tag="LP327_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBL1726723.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            2388..3338
                     /gene="yidC"
                     /locus_tag="LP327_002658"
     CDS             2388..3338
                     /gene="yidC"
                     /locus_tag="LP327_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL1726724.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(3517..3717)
                     /locus_tag="LP327_002659"
     CDS             complement(3517..3717)
                     /locus_tag="LP327_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726725.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         1333 a          947 c          787 g         1420 t
ORIGIN      
        1 aaaagtcgtg tcaaagtccc ttgtttcgaa tgaatggtgt tcaaaagctg gcatctgcgg
       61 taataagccg aaacttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
      121 ttgaagctga ccacttctgt cacaacctca atatggtcta ttattggttt tatctattaa
      181 atcgaggatt ctttcccaaa gtaacgttgc catctcttac gcatacttgc tgacataggc
      241 tgagcggctt ctttgactgc gcagtatttg tctacgaatg ttacgatata tccttctttg
      301 tacttaggcg gtgcaatcgt tgcgccccac atgtgcttca aaatgatatc tcgctcaaga
      361 tccgacaatt cggtgatctt ttctgcattc tttacagcaa ttcttggatg gatatacgca
      421 tgtgtgcctt catcaaattt cgtcgtacgc caatcatagt agaacagatc atgcaaaagt
      481 ccagcacgcg ctgttgcttt agcattgcca ccccattttt ttgcgagcag ataactatag
      541 taagaaacac tgatggaatg ctccaaacga gtggaatgaa catgctgtgt gtaattcgct
      601 aattttttca ctgcttctgt ttctaaaaga tcttctacat aagacatata ttcttcgtct
      661 tcacgccatt tttctgtcat aattatcgat ctgccacctt tttgaatttc attttcttct
      721 gaaattgatc taagtatacc accagtgttc ggattattct agtcgccttg gataaaatag
      781 ccaaagctta atagttcctt aatcataaag aaaaatgata aagaacaatc cctgccgcaa
      841 ccccaacatt caacgattcc gcatttcctt tcatcgggat atatacgttc tgatcagtca
      901 aagacaagat ttcctgactt accccctgac cttcgttccc catgattatt gcgaagttat
      961 ctgatttttc tactttgtct aatgcgtgtg cttcttcatt taattctgtg ccatacactg
     1021 taaacccttt gtctttcatt aaaggaatca cttctgtcaa atctttttgc aacacaggca
     1081 aatgataatt gcttccctgc atgctgcgca gcacctttgt actataaata tctgccgttc
     1141 ctttgcctaa taaaacacca gccatatcaa atgcatctgc cgtacggatc atcgtcccta
     1201 catttccagg atcttgtaca ttatctaaca acagccatcc tcctgaaaaa tctggcgtgc
     1261 cttcagccgt taacgccatc actgctaaga ttccttgtgg tgtaggtaga tccgatacag
     1321 catccatgac ctcatccgaa actagaaaag tagaaatctc tttgtttgcg gccagccagt
     1381 ctccccattc tttcatgcct cgctgtgtca caaaaagttc ttttatttct gcatcagacg
     1441 acgcagcttc ttcaatcaga tggaaccctt ccaaaagata ttcttccttt tcttcccgat
     1501 atttcttttt atgtagtttt tttaattctt tgattcttgg attttttgct gattggatct
     1561 ctttcacatt cctcacctcc ccatgattat atcatggaat aggtgcttga gcttgtttca
     1621 tcctaaaaaa atcggtatca ttaatttata aaataggaaa gaaaggtgac gaacatgcgc
     1681 aaagttaaaa tgaacgtcca aggacgcgtc caaggcgtag gatttcgcta catgaccaag
     1741 atggtcgctg atcaactagg cgtgactgga acggtaaaaa acgaagatga cggctcagtc
     1801 acgatcgaag caatcggcaa tgacgacatc atccagaaat ttattgaaga agtcaagaaa
     1861 tctccgagcc caagtggacg agtgcagtat gtggatgtcc aagaagaccc gatgattgag
     1921 gaacgaaaga aatttgatgt ggtgggataa ggttgtgaaa aagctggtct gcatcaagca
     1981 ttaaggacag gttgtgaaaa atagctctcc atatttttca acaggctgga cttaatgccg
     2041 tagatgcaag cttttgaaca ccgtcgataa ggttattctt gaggcgcttt gcctcaaaaa
     2101 ttaaggacag cagtgaaaaa gctgatctat cacttgcagt gagttcattt gactccgctt
     2161 tttcgggcag aaaaaaagtt tttagtttat taaagtaaga aagaaacact tggaatcatt
     2221 ctactttttc ctagtatttc tttcttgatt ttttgtcttt ttttcaaaat ttttcgctac
     2281 gtaaaagaac gaatatttat atgaaatttc tatgaagttg attgaattcc atgcgctttt
     2341 ttagtatagt taagtttgtg taaaaattat agaagaggaa gtattctatg aataaatgga
     2401 aaaactggtt attgggatcc ggacttgtca gtatcctgct ctttttatct ggctgtgtgc
     2461 ggatggattc aaacggaaac cctgacactt cgggaatcgt ttatcgagta ctcgtccatc
     2521 ctatgggcca agcgattaca tatctagttg aaaatttcaa ttggtcttat ggctgggcag
     2581 ttattgcaat gactgtgatc gtccgtatca tcatcttgcc actaggtatt agtcaatcga
     2641 aaaaaacaat gatccaatct gaaaaaatgc aagcgttaaa accacaagtt gaagcagccc
     2701 aacaaaaatt gaaagctgca acaacacgtg aagaacaaat ggcagcacaa gcagaaatgc
     2761 agcaagtata ccgcgaaaac ggattgagta tgaccggcgg aatcggctgt ctgcctctat
     2821 tgatccaaat gccgatcttc tctgccttgt actttacggc acgttataca gaaggtatcc
     2881 gggaatcatc tttctacggc atcgatttag gctcaccaag tatggtactt gttgccattg
     2941 caggggtcgc ttatctgctt caaggataca tctcaacgat cggtattcct gaagaacaga
     3001 aaaagacgat gcgtacgatg ctgatcgtct ctcctgctat gatcgtcttc atgtcgatca
     3061 gtgcgccagc cggtgttaca ctttactggg tagtcggtgg tgtgttcagc tgtctgcaaa
     3121 cattcatcac aaacgttatg atgaaaccac gtttgaaagc acaagtggca gaagagttga
     3181 aacaaaaccc accaaaacaa gtcgtcactc caagaattga tgtgacagaa tcagctaaac
     3241 cagctgacag caaacaagca accaaacaaa tcaagcaacc caaaaacagt acaaacaata
     3301 aaggccgcaa cgcaggaaag cagcagaagc ggaagtagat agtaaataag agtgtaataa
     3361 aaattcatta acagttttag caattaaaaa tatcgaccaa gatttttgaa aaagaaatct
     3421 ggtcgatatt tttatttaag tgaatgttta atttgaatca tcgattgatc gctatctaat
     3481 tgcagcagca tctttttatg aagaaacaac tttttcttat ctatcaacgt agacaggtgt
     3541 tgctgtttct tctaatagtg tacttaattc cttaatattt tgttttcctt cttctaacat
     3601 ccaactactc gctttttctt cgccttcggt aacaaaaggc gccactccct tcgcccatgt
     3661 tgctcgccca cataagacac cattctccat aaaggacaac gcggagatac cacccatcta
     3721 aataacatcc ataaaccttt ctagtagaag aatagtgata gtatccatcc ctttttctac
     3781 tgggaataca attacacgat tcaaagcttg atacacaaca tttcccttct tataaaccgc
     3841 tattgtaacc gaattgtttt gaatcacctt tttgggatag aaactggctc ctttccgcaa
     3901 cttcttaaat gttggtttct tttatacttt ctttgtagca actacaaatt tcttcaaatc
     3961 tctaaatgtt tgcataacaa ccgcctttct gacttttact caggatggta tttaaaaatc
     4021 agcaaaataa ggcttctctc catctttttt atttcctagc tgctttaaag acactcttgc
     4081 tgttttttaa tctctatcaa gggggctctg aatctatttt gatgaactta tcttattaat
     4141 ttctcaaatg agctgtcaga cacaactgta ttttttcaat ctcataccgg acaactatgc
     4201 cagcttaaaa aaatttcctc tggcaaaact tctttattga aaaatgacaa tgaaaaaaga
     4261 ccattctttt tgttgaatgc tcattgtgta aacatatttc ctgcgactgc tcttcgaacc
     4321 attctcagtg caatccctaa tttatctatt aaatcttctt ttagttcctc atctttcata
     4381 ttcaaaattt cctttgtatg ttacacaagt ttgttctcta aataacgcga attgtaaaat
     4441 taagttagaa aaataaaaag gcatttatgg tacactcaaa ttgtatt
//
LOCUS       DAFEHS010000125         4308 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFEHS010000125
VERSION     DAFEHS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.131"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(52..825)
                     /locus_tag="LP327_002660"
     CDS             complement(52..825)
                     /locus_tag="LP327_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726726.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            1092..2792
                     /locus_tag="LP327_002661"
     CDS             1092..2792
                     /locus_tag="LP327_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBL1726727.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2762..2980
                     /locus_tag="LP327_002662"
     CDS             2762..2980
                     /locus_tag="LP327_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBL1726728.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            3000..3890
                     /locus_tag="LP327_002663"
     CDS             3000..3890
                     /locus_tag="LP327_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBL1726729.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3908..4192
                     /locus_tag="LP327_002664"
     CDS             3908..4192
                     /locus_tag="LP327_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726730.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1500 a          723 c          851 g         1234 t
ORIGIN      
        1 aatcatgtcc tagtttcttg tttccttttt attttttgat aagaaataga tctacttcaa
       61 tggcagataa gaaagatcgg catcttctac caattgccag tgtcgatcgg tgacagagta
      121 gattaaagta gcgatgattt cctcttctcg ataccgggtg ccttttaatt tccaaatata
      181 atcttcaatc acatagattg ggatacgata tttaccatcg atcaaaccaa cttgatcaaa
      241 agaaaaaatg atcccttcct gcatcaaatt acgcatttct ttcaataatt ccacactgat
      301 tttttcaaca ggtgtttctt ttttgaaaaa agattttttt acattgatct tatctgacaa
      361 gatcgaactt aatttactag caaaaatcct ataaaattca tctaaatagc gataataaat
      421 ccgattcagc tgatcattaa gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa
      481 cggcgaatga agatgtgttt tcatatgacc aaaatataat aattctgaaa tttctaaagg
      541 tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc
      601 acgcctgtta cgagaagagg aaaagaaacg ctgaatattt tctgtaccac ggattacttt
      661 cagccctgta tgggattcat attctccagc agaagcagat ggatcaagca agagcaagtt
      721 ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac
      781 agcattactt gtggtatcaa tatgtacata aactgactca gtcatcatgt tcctcctctt
      841 atttcctctt aatctctttt attttacaac atttgcatta aaaaagcttg tttcttttat
      901 tttaaaattc cattacaaaa tcacgttttc attgaaaaca ctctcatttt caattaattt
      961 ttgtgatttt tgtatttttt tatttaaagg aggttttatg aattgttttc ttatggaatc
     1021 tacaaagtcg aatacgagac tcatgacctt tttatgacgt actggtatac ttgaagaaaa
     1081 aggagagcgc gatggagata aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc
     1141 ctatccagca agcaaaacaa ccccttccat tttttcaaag tcatcaagaa agtagagacg
     1201 ctttttggcg ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa aaatataccg
     1261 tctattttac tattgctaaa agtagaggag atgttatttt taataatctg ttgattgaag
     1321 gtgaaacttt tgaatggaaa aaattttttc ggtatatgga agcttgcgct cgttttttta
     1381 ttaaagaaaa ctgttgtttt ttatttcaat ctcctctatc agaagaatgg ttgcgtattt
     1441 ttcataaaaa cggctatcaa ggaaccacac aattaaataa aaagctggtc tatcatacag
     1501 cacttgtttt aggtggagga ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc
     1561 tgaaggaaca tgatatcaac tttgagatta tcacaggtac ctcggtaggt gcgttgaacg
     1621 gcgcattgat tttacaagga gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga
     1681 caagacaagt gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt gaacgcttct
     1741 atcgagaaag acgccaaatg acgaaaacag cactaattga aggcggggtt tcttctgctc
     1801 cattggaaaa gatggtcaaa gacaatttgg atttgtcctt actcaaacat aatcccaaaa
     1861 tacgtttata tacggtgtct actaaactgc cagaactagc ggaagtagtg actgatatcc
     1921 aacagacaaa aacagaagaa ataccagatt ggatactcgc ctctgcttca ttttatcccg
     1981 ctatggctta tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa
     2041 tacctattga tattgcgatt aacaaaggag caacagaggc atttgtcgta gatgtgcaag
     2101 ggccaggccc agcgaaaaaa attcgtatgc cagacatttt tatccattgg aaatgtcaaa
     2161 cattgtggac actaggcagc tttctgcttt ttgatagcca aaggaaccaa ttaaatttac
     2221 aattaggcta tctggaaatg aaaaaacgat taggctatta ctatgggaac tggtatacat
     2281 ttcattctgt taaacctgca agaacatact ggcgaggatt tttgtcttac ttaacaaaag
     2341 aaattcagtt agaattatct tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt
     2401 tatataagaa cagggtcgta ccagagacat gcggattggc gatgctagaa cttttagcaa
     2461 aaaaacattt tttactgccc aatgaaatct atcaagtaga catgatgatt caaatgatct
     2521 gccaacaaga tataaaaagt atgccggatg atttattggc acaaattggt caattaagta
     2581 ctgaagaatg gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa
     2641 ctgtttattt tgattatctg ctacaaggaa aacgcaagga tcgtttgcag catgaattgc
     2701 ttaagcaacc agttgacaca ttactgatcc tatatttata ttatctcaag gaggaacaac
     2761 catggcacaa gaattttcat atgaaatcgt agaagaaatc gctatattgt cagaaaacaa
     2821 caaggggtgg cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc caaaattcga
     2881 tcttcgagat tgggcgccag accatgaaaa aatggggaaa ggcctgacat tgacgaacga
     2941 agagtttgaa caactgcaaa aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa
     3001 tgaaaagtat gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat cagttagata
     3061 ttgaaataaa aagtgtcaac cagcgttttc tagatgttca aatacgaagt cctaaaatcc
     3121 tgaactttgt tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa
     3181 tcgaagtatt tgtgaatcta gcttatattg gcaaaaacga aaaacagatc gtcatcaact
     3241 gggaattgat tgattcactt atcactcaat tgacagacgg aattcaacaa cgatacggta
     3301 gtcaaaatca agtgtcaatt agtcggatgg cggaacgctt cgcgctggac gaaaacttcg
     3361 taacggtaga agaaaaaccg gaagagagca tggaagaatt ggcacagtta gctctagata
     3421 cattgaaaag cgcacttgat tctattgagg cgagtcgaga aaaagaaggt caagctttag
     3481 ctttagtgtt gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt
     3541 ttgtagaaat ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg gaggaatttc
     3601 ttggagcgaa agtcgatgag caacggttgt tgacggagct agccatcttg ttggaacgcg
     3661 gagatattca cgaggaactg gatcgtctgg cgatccatgt acaaaaacta gatgaactta
     3721 tcgaagcaca atgtcctgta ggtcgggaat tagatttcct gatccaagaa atgaatcggg
     3781 aaatcaacac gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt
     3841 ctaagacgat cttagaaaaa atcagagaac aagtacaaaa tattgaatga cgaaagaggt
     3901 agagataatg aaatattatg acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa
     3961 acgggatatc ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct gtgcagacta
     4021 taatgagaac gaactgttta ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa
     4081 atttattgtt cgaattgaca cagaagaggt agaagatccg acagaaaaag cacgttctcg
     4141 aaaagatgag atcatgggtg tagtgaatac tttgtccaat atgggctttt agcaaggatg
     4201 ctgaacaaag aaatacaata aataatcaga attggatttc ttcgcttagc ttctttgctc
     4261 taggaataca tttaaaggtt gtgacaaaat tcgtgtcaca atcttttt
//
LOCUS       DAFEHS010000126         4153 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFEHS010000126
VERSION     DAFEHS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.132"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..106
                     /locus_tag="LP327_002665"
     CDS             <1..106
                     /locus_tag="LP327_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL1726731.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="LP327_002666"
     CDS             complement(359..508)
                     /locus_tag="LP327_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726732.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="LP327_002667"
     CDS             970..1182
                     /locus_tag="LP327_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726733.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="LP327_002668"
     CDS             complement(1460..3259)
                     /locus_tag="LP327_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL1726734.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="LP327_002669"
     CDS             complement(3383..3979)
                     /locus_tag="LP327_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL1726735.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DAFEHS010000127         4116 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFEHS010000127
VERSION     DAFEHS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.133"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            155..316
                     /locus_tag="LP327_002670"
     CDS             155..316
                     /locus_tag="LP327_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBL1726736.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..1612
                     /locus_tag="LP327_002671"
     CDS             335..1612
                     /locus_tag="LP327_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1726737.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="LP327_002672"
     CDS             1622..2278
                     /locus_tag="LP327_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726738.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="LP327_002673"
     CDS             2278..3654
                     /locus_tag="LP327_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1726739.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3784..>4116
                     /locus_tag="LP327_002674"
     CDS             3784..>4116
                     /locus_tag="LP327_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL1726740.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1628 a          453 c          727 g         1308 t
ORIGIN      
        1 ctgcgcgagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagagag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaatta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa ataattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt taggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg tagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     3901 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     3961 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     4021 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     4081 ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DAFEHS010000128         3884 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFEHS010000128
VERSION     DAFEHS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.134"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            275..2170
                     /gene="asnB"
                     /locus_tag="LP327_002675"
     CDS             275..2170
                     /gene="asnB"
                     /locus_tag="LP327_002675"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBL1726741.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(2365..2811)
                     /locus_tag="LP327_002676"
     CDS             complement(2365..2811)
                     /locus_tag="LP327_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL1726742.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            3039..3188
                     /gene="rpmG"
                     /locus_tag="LP327_002677"
     CDS             3039..3188
                     /gene="rpmG"
                     /locus_tag="LP327_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL1726743.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            3276..3794
                     /locus_tag="LP327_002678"
     CDS             3276..3794
                     /locus_tag="LP327_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL1726744.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
BASE COUNT         1297 a          646 c          741 g         1200 t
ORIGIN      
        1 gacacgatct ttaggtcgtg tctttttttg ctggtttttt tggaaatagc atccactaat
       61 atttttttaa cacgaagaga ataaggtagc ctcaaaaaag aaaacatggt aaattagata
      121 ttgggacttt attttctgta aaaacagaaa ctttcatctt ttgttttcac gtgacgaaat
      181 tgtaagagag aaaatattcg tccgctattt attctatgat aagatgcata tgaatgaaat
      241 aaacagtaat tttatattag gatgtgaaag ttaaatgtgc ggtattgtag gttttgttaa
      301 cgacaaggac aacaaaaaaa caattattaa tacaatgatg gatcgaatcg ttcaccgtgg
      361 acctaatagt tcaggagaat atatcgataa acatgttgct ttaggattca gaagattaag
      421 tattattgac ttagagggcg gtactcagcc tatttacaat gaagatcgga caaagatcat
      481 tatttttaat ggcgaaatct ataattacca gcctttgaga gaagaattga ttgcagctgg
      541 ccacgttttt caaacacatg cagatacaga agtccttttg catggttacg aagaatgggg
      601 aacagaatta ttgcaaaaga tccgcggaat gtttgctttt gctatttggg ataatgaaaa
      661 gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa ccatattatt atgcagaaat
      721 gaacggaaca tttatgtttg gttcagaaat caaaagtttt ctgcctcatc ctgattttaa
      781 caaagaattg aacaaagaag cgttgaaacc ttatatgact ttccaatatt ctccattaaa
      841 cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa ggccattatt atacgtataa
      901 agatggaaaa atagacattc agcaatattg ggatgcagat tttgaaacaa aagaaacgca
      961 ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt caagcttcta ttgaagcaca
     1021 tacggtcagt gatgtagaag ttggttcttt cttatccagc ggggttgatt ccagttatgt
     1081 aacctctgta ttgaaaccag atcattcttt ctcaatcggg tttgatgata aaacctataa
     1141 tgaagcgatt gaagccagaa aactaaccga actattggac ttagataata cagcagcagt
     1201 gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc cagtatcact tagatgaacc
     1261 tgattcaaat ccttcttgcg tacctttgta ctttttagca aatcttgctt cacaaagtgt
     1321 gcgggtagtc caatctggag aaggggcgga tgaactgttt gcgggatacc aaacgtatgg
     1381 attccatacg aattcaaaat tcatccgtgt gattgctcaa ggattgaaaa aattaccaaa
     1441 aggaacacgc tataatttag gacgtaaaat cggaaaaatg aaaaacttcc atggacggat
     1501 ccatttatat gaatcacttg ctcctgcaaa agaatatttt atcggtcatg ctcgagtgtt
     1561 cgaagaatca gaagcatcag aagtgttaac accaaaatat caaacagcac catctgtgga
     1621 tgaaatcatg actgttcatt acgagaaaac agaaggaatc aaagatgaag tcaataagat
     1681 gcaatatgtc gatcttcatc aatggatgcc aaaagatatc ttgttaaagg cagataaact
     1741 ttctatggca agttctttgg aagtccgagt acctttattg gatatcgaag tcatgaaatt
     1801 ggctcaacaa ataccaagta agtttttatt gaatcaaaac aacacaaaag atattttcag
     1861 acaagctgcg aacaaacatc ttccggaaga atggtcgaat cgtgtgaaat taggattccc
     1921 tgtgccaatc aaagcttggt tgaaagaaga acacggctac gaacaagtca aagcattatt
     1981 cgaagcagat tttgcaaaag aattttttga tcaagaaaaa atcatgaagt tgctagatga
     2041 ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg acaatcttca gtttcttgac
     2101 atggtataaa gtctactttg tagatgaaac gattccgcaa gcagaagcaa tcgactatgt
     2161 gactgtttga tgaaaatcaa atatcaataa aaaagaggtt ttctcctacc gtttaacact
     2221 gagtgataag aaacaatttc aaaaaacagc tcttcccgtt ttatagatat tatgacttat
     2281 ggttcggagt tggcagagga acaccattgt ttcgaacata agggggttgt gacacgattt
     2341 tcgtcacaac ccctttacat atgttcaata atcgatttct ttttcttttg tcccatcttt
     2401 tttataaatg aacaaagttg attctttgtt ttgtgctact tgctttcctt tttcgattgc
     2461 ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc cctttagatt tcacgatcca
     2521 ttgttcttcc tcatattcta cgatcacatc tgaatcaagc agttttccag cacgaggatt
     2581 cgtatcgtgt tcatccgtct tgcttggatt tttttctttt tcgaaagttc gtttttctga
     2641 ttcactagca tcttcatacc atttttttgc ttgatcaatg gcaattggga tcgcacggtt
     2701 atcagggtac ccttcatcta ataatgcatt tgcgatgtcg attgcttttt ttcttgtcaa
     2761 tttatctaag tttttcattg atgcaggaaa gtccttcatg ttccatggca tattagtatc
     2821 cccctttatt aaaaaacata atctatataa acaagtgtaa gggtatttat acaaaaagaa
     2881 aaagattttg tttgagaagt aaaaccgttg tatttatcgt caagtagttt tacttaacaa
     2941 aatcgtgaaa aacactagca atctagaaaa aatcatggta agataatcaa gtctgagagt
     3001 catcttagag gattcttgta aaaatgggag ggaaaaacat gcgcgtaaac atcactttag
     3061 agtgtacttc ttgtaaagaa cgtaactatc taacaagcaa aaacaaacgt aacaatcctg
     3121 atcgtttgga aaaacaaaaa tattgcccac gcgaacgcaa agttactttg caccgtgaaa
     3181 caaaataatg tttttaaagt tctcaattcc ttgctggtca aagaattgag acttttttta
     3241 tctcaaaaca tctaaaatca taaggagcgg ataatatgtt tatttccatg tgggcacaag
     3301 ataaaaatgg tctgatcggc aaaaatggtt tgttaccatg gcgattgcca aatgatatgc
     3361 gtttctttcg cgaacataca atggatcgaa ttttagtgat gggaagaaag acctatgaag
     3421 gaatggggga tctttcactt ccttatcgcc atatcatcgt gttgacaacg caaccaaatt
     3481 ttcgcacgaa agaaaatgcg gaagtaatgc attcgatcga tgaacttttg tcgtatagtc
     3541 aaacgatctc agaagatatc tatgtttcgg gtggtagtcg gatttttcaa gaacttcttc
     3601 cttacactgg tcggatctgg cgaacattga tcgatagcac gtttgaaggc gatacgtata
     3661 ttgggaatat tgatttttca gacttcgccc ttatagagga gtatgagggg ataacggatc
     3721 aagaaaatct atatgcccat cgttttcaaa aatgggaaag agtaaaaaat agggttgctt
     3781 caaaagaaga ataaaatcta tgcgtctagt ttgttaataa gaaaaaaagc atctcacgac
     3841 taatcgtgaa gatgcttttt ttagttaaaa tcaatttagt tttt
//
LOCUS       DAFEHS010000129         3806 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFEHS010000129
VERSION     DAFEHS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.136"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..321)
                     /locus_tag="LP327_002679"
     CDS             complement(<1..321)
                     /locus_tag="LP327_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL1726745.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSART"
     gene            1063..1380
                     /locus_tag="LP327_002680"
     CDS             1063..1380
                     /locus_tag="LP327_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL1726746.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1471..1836)
                     /locus_tag="LP327_002681"
     CDS             complement(1471..1836)
                     /locus_tag="LP327_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726747.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2044..2526
                     /locus_tag="LP327_002682"
     CDS             2044..2526
                     /locus_tag="LP327_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726748.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2723..3613
                     /locus_tag="LP327_002683"
     CDS             2723..3613
                     /locus_tag="LP327_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBL1726749.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDDATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1290 a          594 c          696 g         1226 t
ORIGIN      
        1 ggtacgagca gaacaactaa taggaaacgc tgcacgacca acaatcacat cgggtgtcca
       61 tccttgaaca acctttcttt gaatatattc tgattgttcc tcgggtaaaa caagcggacg
      121 tctaccacag tttgatttgt ttttcttata ctgttgataa tattctaagg ctgattttcc
      181 ttgcttgaag aacagatata ctttatggat cgtttgtctt gaacgattca agcaatgggc
      241 agttttagca acagattgat tcattttgaa ataagactct attattacaa gttcatccgt
      301 tgtaagatgg gtataggtca tttgtactca ctccttataa ttctttggtc ggaactattt
      361 tgagtgtagc acaaatgact tttttagttg tctagcttaa ttttacaatc ggcgacttat
      421 tgcttttgca aaatttattt acctgttttc tggatcaacg atagtgtaca ttatgctatc
      481 cttgtttgga ttttaggacg tttaaagagg cactttttta cataacttaa tccatagacc
      541 ttttaagcat gctgtgagat aataataaag aagagtttaa agcgcacccc aaaccacttc
      601 cccataagtg tattacgctt taaactcttt tatatttgaa ttcactaaga agcataccat
      661 atatttgaat ttactaagaa gcataccata tttttgaaaa aaagtgagaa aaaaggctta
      721 taattaggat ttagagtaat taattagatg tttattttat aggactgata ctatagatta
      781 caaatagtat cgtatttcgc aatcttaaaa ttatcttctt actgtgaata agataataaa
      841 atttgtattt gtccttttta ggtaacttat tttaatctat atacctttct ggatcaacga
      901 aagtatactt tatatagtca taacgccgat ggtaactatg cactgtaacg ataatgctgc
      961 taaaatatct ttttacgaaa aacatcacaa ggaaaaactt aactatggta ttatctaaac
     1021 ataatttttt cggtatttaa tatttaatta ggaggtaata ttttgctttt ttatagtaat
     1081 tttatcttaa tagtagcgat attattactt ctgaatatat ggatttttga taggagtaga
     1141 aattcaagta taggttttag aactaaacga agtttgtctt ctaaaaaaaa ttgggtatac
     1201 tcacaaacta ttttttatgg aggaattgtt ttaattagtt tactttcttc aacactttat
     1261 tctttgaata ttatagatgt ttccacctca aattcaatat ctattattgg aatcattatt
     1321 gctgcaatta ttacacaact gtttttagtg tttggagaga agaaaagaag taaaaaatag
     1381 aaaaactagt gatagttttg gtgatataag aaaaacatat atgtataaaa agaagaaagt
     1441 agcttctctt tgtacatata tggtttgttt ttatggatgc cgagcttttt tataagataa
     1501 ggctaccgcc cataacgacc caaggatttg tggaactaag taaatacctg caagaagcaa
     1561 gatcagctgg gtcggtttta aaggatgata gaagtaacgc cattcagtaa aattggcgca
     1621 aaataaatag gaagtgacag tagatataat attggcaatg aataaagata taatacgcct
     1681 gttcctttga aaataatatc cagcaaaaag agataaaaat atcaatgtga tgaacccgct
     1741 tacggaatga taacgaaaat caagcagtaa ggcgaaatgc acataaggaa caaaagataa
     1801 aagcaacgga aggtttttcc agcaagtttt tatcatcttt catcaccacc taacaagtgt
     1861 tattattttg ttagttttat tgtaaattga atcctgcaat aatcaattac tttttcatta
     1921 tttctagatt aaatgaataa actctcatgc cattatctct aaaaaaaaag tttttccccc
     1981 taattttttt agatagaaaa ttgtatagta tgatgagaga aaaagtagtt tgggggaacg
     2041 ttaatgagaa gaaaagcagt aatcgatcgg ctttatttat ttttttggtt ttttcctatc
     2101 gttttgctac cttttacatg ggtaaatgct gttgtacaag gtgcggagat attaggcctg
     2161 atccaaggaa gcggatttgt ggtttctagg aattttccag ctttgattgt tttctattta
     2221 gtgataagtg taggtctctt attaccaaaa tctctgatca atcactacaa ttggcagctg
     2281 ctttactttt cttgcttatt agtatttaca tctatgtttt ctttgctgcc tcgcttgatt
     2341 atcggcccta atggtttgag cgagagtgga ttgaaggatt tagcttattc ctccatgtat
     2401 ttcttgacga tcaaaccatg gttctattta gtaattgcct catttattct tgcaacattt
     2461 ttttacttct ggacagaaat caggataaaa agtgaacgat ctgtatctat aaaaaataac
     2521 tattgatggt ggatcatcag taaaaaaaca tatgagctgc gtaaaatgca tgtttctcat
     2581 atgctttttt tattattatg taaagaatgc gaaaaacgtt tacatgattt ttacataact
     2641 taactatatt tttacagcaa ccatacgagg gatttacagt cgactgataa actttagttg
     2701 taaataagaa aggagcaata aaatgaaaaa agtattgttt actctaggta tgatgggaat
     2761 tcttttagcc ggatgcggct caggatcagg aaatacagat ggctctgcca cacaaaataa
     2821 ttcaagtgac tcaaatgaac aagtaaaaat cgttgcggta ggttcgacag cattacagcc
     2881 gttagtcgat gcagcacaag aaagctttgt acaagaaaat ccaaattatc aaatctctgt
     2941 acaaggagga ggaagcggaa ctggtttgag ccaagtcgaa gctggagcag ttactatcgg
     3001 aaactctgat gtatttgcag aagaaagaga cggagttgat gcttcaaaat tagtggatca
     3061 taaagtagca gtggtcggga tggcaccaat cgtcaataaa gatacagatg tcaaagatat
     3121 caccaaacag gaattgattg atatctttac aggaaaaatc actaactgga aggaagttgg
     3181 cgggaaagat caaaaaatca acgtcgtgaa ccgtgcaaac ggcagcggta cccgcgcgac
     3241 attcgaaaaa tggggactgg atgatgctac ccctgtgcag tcccaagaac aagattcatc
     3301 aggaacagtt cggcagctgg taagtcaaac accaggagca atcagctatt tagctttctc
     3361 atatctcgat gattccacac aagcactaag tatcgacggt gtagaaccaa aagaagagaa
     3421 tgtagcagat aacagctggg gaatctggtc atatgaacat atgtacacga acggaaaacc
     3481 ttcccctgaa gtccaaaaat tcttagacta catgatgaca gaagaaatcc aagaaggacc
     3541 tgtcaaagaa ttgggctatt taccaatcac aatgatggaa gtagaacgtg atcacgaagg
     3601 aaatatcaaa taactagcag ataaacatgc cacggtagtc gttactatta aggaaaaatc
     3661 aactatgttt ccccacatat acatttttcc tttaattgtg aaaaatagtt cctcatattt
     3721 tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttat tcgaaaaaaa
     3781 gagctacgac atagaactgt cgtaac
//
LOCUS       DAFEHS010000130         3789 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFEHS010000130
VERSION     DAFEHS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.137"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(71..448)
                     /locus_tag="LP327_002684"
     CDS             complement(71..448)
                     /locus_tag="LP327_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBL1726750.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(494..682)
                     /locus_tag="LP327_002685"
     CDS             complement(494..682)
                     /locus_tag="LP327_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBL1726751.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            853..1416
                     /locus_tag="LP327_002686"
     CDS             853..1416
                     /locus_tag="LP327_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1726752.1"
                     /translation="MKFGGHFLNKKQDQEYYIEKLEKENLELKERIRYYETKFCKRGD
                     CMKPNPVETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERGFSLPNKERLIKIA
                     ELSNKTIDWIKWGTLEEYITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDK
                     DYELLDSIIKNIFTKYYSKEFEVTQIP"
     gene            1430..2212
                     /locus_tag="LP327_002687"
     CDS             1430..2212
                     /locus_tag="LP327_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726753.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            2252..2863
                     /locus_tag="LP327_002688"
     CDS             2252..2863
                     /locus_tag="LP327_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL1726754.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
     gene            3307..3456
                     /locus_tag="LP327_002689"
     CDS             3307..3456
                     /locus_tag="LP327_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726755.1"
                     /translation="MKLEIKEVVCDWGIYVDGETYPFMIFNSKANAQEIMRIMELDNK
                     HERFD"
BASE COUNT         1441 a          506 c          692 g         1150 t
ORIGIN      
        1 agtactattt tacttttaat tccgctatct atgtcattag gcaactaaat tgttattaat
       61 atatttacgt ttactcgtat atttttagta acgcatctgt caaaacttta gaaaaattta
      121 ttttatcttt ttcccctcta gctactaacc attctgggac tgtgacgttt ttgcgtacgg
      181 tctttttttg gataaatgga gaaggatcac tttgaataag agaaacaatt ttgttattgc
      241 ccaaattatt tagttgatga atatctgttg gagaaggaac tgaaatatct tcatcaatat
      301 agtaagctag aatcccatca agaaattctt tagcattgga catagcttct tcaaaattat
      361 ctccttgtgt tgctccagca aaatcaggaa aaactactga gtagccattt tcttcagcag
      421 aaaaaattac agggtaactt tttttcattt ccaccactcc tttcgagtta atccaatgaa
      481 ctaaaggact gggctatttc agccctaagt cctttagtaa cttctgctct gtcccttttt
      541 ttaaatcctg attactatga acaggaatag gaacgggtgg gaatccttct ttggctacaa
      601 tatgatggct gccagttatc ctcaccactt tccaaccatt tttcttggct agtttgagca
      661 tttttttacc ggtaagtggc attcgcgact acctccttac aaatattatt atacgcatta
      721 tgcgtataaa ggtcaagagt ttttacatta aaagtagaat gtaagtgtca caatagggta
      781 taccctattg tatcatatgg taaagtacag tataataagg tttggaggtg taacactagg
      841 gtgtaacaca atgtgaaatt tggaggtcat tttttgaaca agaaacagga tcaagaatat
      901 tatatagaaa aattggagaa agagaactta gaactcaaag agcgaataag atactatgaa
      961 accaaattct gtaaaagagg tgattgtatg aaaccaaatc ctgttgaaac aggtaaaaga
     1021 attaaatcaa ttcgttctaa ttttggaatg actatggaac aatttgcaga tttaattgga
     1081 aatgctcaaa aaggaaatat taataattgg gaacgaggat tctcccttcc caacaaagaa
     1141 agattaatta aaattgctga attaagtaac aaaactatcg actggattaa atggggaact
     1201 ttagaagaat atataacttc ataccttatc ggtataggat atgagctata tattaaagac
     1261 tttccagaga tacctcacaa agtctttaaa gacattcaag aaaaatatag tgatacgttt
     1321 tcgttagata aagactatga attattggat tctataataa aaaatatatt tacaaaatat
     1381 tactcaaaag aatttgaagt aacccaaatt ccataagaga ggtgattgta tgaagccaaa
     1441 tccttcagaa gcaggaaaac gaatcaaaca attacgcctt tcatgtggtt ttactatgga
     1501 agaacttgga aggaaaatag ataattcacc acgtgcgacc atatccaact gggaacgtgg
     1561 gaccaattta ccaaatccac aaaagctgaa attattatca actatcacta attcaacaat
     1621 tgactggatt aaatggggaa ctttagaaga atatataact tcatacctta tcgatatagg
     1681 gtatgagcta tatattaagg actttccaga aatacctcac aaagtcttta aagacattca
     1741 agaaagatat agtaatacgt tttcgctaaa taaagactat gagttattga atcctataat
     1801 aaaaaatata tttacaaaat attactcaaa agattttgaa gattatcgag acatagaagt
     1861 taaaccagac cctaaaaagg tagggagaaa aattcgttct attagaaaaa aattaggact
     1921 tactatgcag gaatttgggt acgaggtttc taattctcct agaagcactg tgtcaacatg
     1981 ggaacatggt ggtaacttac cgaacaaagc caaactaaaa aaaattgcag acattgcgaa
     2041 ttgtagtgta gaagatttgc tttttgatga aggctttatt tctgaaaaat cccaagttaa
     2101 attgataaat gaactcaaag aagaaaattc aaagctaaaa gaaaaaataa aatactatga
     2161 atgtttattt gatggtatag agagtctgct gaactcacga aaaaattcct agttaattaa
     2221 actagagtaa aattatttgt aggtgttaaa tatgttagtt ggctatgcac gagtcagttc
     2281 agatgatcaa aatttggaaa gacagcttga agaatttaga aaaattggtg tcgaaaaagt
     2341 atttgctgag aaaaaatctg gacgtaacac agtagaacgg ccagagttca ataaaatgtt
     2401 gtcatttgtc agagaagggg atacgcttgt ttttgagtcg cttgaacgct taggaagaaa
     2461 ttctgatgaa atactagaaa cagtatcttt tatagataaa aatggtatgg gcttaatggt
     2521 tttgaattta ccaattttag atacaaaatt tggtgatcca aacttagaaa aacttgttag
     2581 aagcttagta attaacattt tttcttggac ggctcaaaat gagagggagg aaattaagag
     2641 aaaacaaaaa caaggaatag caattgcgaa gaagaaagga gtctataaag gaaaacccta
     2701 cgaatattct tctactgcaa agaatccaca aaagaggttt atctataatg aaatagtaag
     2761 aatgcttgga gaaggtgttc cgattaagcg aattgcagaa aaaacaggaa caaatagaaa
     2821 tttagtctat agaataaaga aaagaataag tgaatctgat tgattttaag ttctttgaga
     2881 aatcaatgtt acgagagaag gattctaagc ctgtttcaaa cttttaaggt gtacaattat
     2941 acaaagaagc ttctaaacaa aagaattaat tttgtggcag tcaagtaacg acagaaatag
     3001 aaaagaaaga ggctatgcga gagtgaatca acaagttaat tgggttaatg attttgaaaa
     3061 agaatttcga ctgaaatttt ttggaaatcc tacagcagaa agtacagaag aaaaaaatta
     3121 tgactggtca gaattttagg ttgcatattc gattcaataa gtgttgaaga taatagttct
     3181 cttacgccac agtatacaac gttagaatat gacgaaaaca gattcaatac atttccttgc
     3241 aataccattc aagaatcaat tgataagcat gagtgataat tgattatcta caggaaggtg
     3301 ttttttatga aactagagat caaagaagtt gtctgcgatt ggggaattta tgtagacgga
     3361 gaaacatatc catttatgat ttttaatagt aaagcaaatg cacaagagat aatgcgtatc
     3421 atggagttgg ataataagca cgaaagattt gattaagtaa aaaaaatgga gaatgaatgt
     3481 gaagcagtgg aataccaaaa aacatgcgcc aataataagt atattttagg tgcaatggga
     3541 ttaggtaaat cagcttctat taaacaggtc tacgaagaat cacaggagaa ttgcgtatat
     3601 gaaaattcga attaaagagt gagaatatgt gggagctaat ttatttagtt ttatacttaa
     3661 taggaaaatc aactgaattt gtatataaca ataaggtaat atttatattg ttcttaatag
     3721 tttgtattat cgtatttgat gtctatgatt tactaattca taagaatgaa aatagtaata
     3781 ttggtagaa
//
LOCUS       DAFEHS010000131         3645 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFEHS010000131
VERSION     DAFEHS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.138"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            108..1307
                     /locus_tag="LP327_002690"
     CDS             108..1307
                     /locus_tag="LP327_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL1726756.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(1334..2602)
                     /locus_tag="LP327_002691"
     CDS             complement(1334..2602)
                     /locus_tag="LP327_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBL1726757.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(2765..3411)
                     /locus_tag="LP327_002692"
                     /pseudo
     CDS             complement(2765..3411)
                     /locus_tag="LP327_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
BASE COUNT         1072 a          767 c          636 g         1170 t
ORIGIN      
        1 gaaaaacatc tttccatcgg aaagatgttt ttttgctttt tctagtttaa ataacagctt
       61 ttttcatcta ttttcgttat actgtataag aaaaaggagt tgaatttatg aatctaacca
      121 gagatctgat ctttttagtt ctctatctta ttggggtatt ctctatcgtt tattggcgaa
      181 aacagaagaa ggatccttcc ttgttctttt acggcagtac gtggacattt acggtaatct
      241 taacttattt tgtcgtgttg gaaatttttt attcgacctt ttattttttg attccattgt
      301 tgttctttag tatctttgct ttttcttatt tcacagaaaa acgaaggcta ctgaatggtc
      361 ttttattcaa tgttttactc atcagtttcg gcatctactt gtttgtcttg ctgtatgaaa
      421 cgcaaaatat ttttcttggc ggactgatcg ccttgatcac gattccattg ctacttgtcc
      481 ttttatttgg tatttatgga ttgattgttt ttcttttttg gaacggggtc acagttttac
      541 gtagagaatc gcattcatta gctaatcttt taacgttgat actagccatt tttcttacac
      601 tgtttcttgt ttttgacttt ttcttgttaa aatatttgcc gcaatggata aatgctttgt
      661 ttttctgcgt tcccctgatc ttgatctatc tatttatcgt attctacaac tttttgactg
      721 tctcattctt gtatcagttc aaccggcctc gatacaatca agacttcatc gttgtgttag
      781 gcgctggatt gatcaatgga gaaacagttt ctcctcttct agccaagcga atcaataagg
      841 caattgcttt ttatcgggct caaagccgtg ctacattaaa tccgcccatc ttattgatgt
      901 ctggcgggca aggagcagac gaaaaagtac cagaggccat tgcgatgaaa caatatgcca
      961 tggaacaagg aatccctgaa agagacattc ttgtcgaaac caattcgaca accactttgg
     1021 aaaacatgct ctattcaaag gaaatcatgg atcagcaaat gaaagggcca taccgcgcga
     1081 tctttagttc caacaattac cacattttta gagccggtct ttatgcgcgg caagccaaat
     1141 tgaaagcaaa tggtattggt gcaaaaacag ctttctacta cttgcctaat gcgtttttaa
     1201 gagaatacct cgccatcttg atgatgaaca aaaaaagaca tatgatcatt tgcggattgc
     1261 ttttcgcagg ctccgcattc ttatcgctga ttactttact tttctaaaaa aacaaggtgt
     1321 gacaaaacat atgtcacacc ttgttttttt ctttcattct cttaactatt ggcaaaaaga
     1381 gtatcccttt tttgaccgct acttgctgag cagaataaat accgctgttg cctgaacaca
     1441 taaagctgat caagcagatc ataaagaagt atactgccgc ttgtgaaccg aacaattcga
     1501 tccccatcac aaagcaggca atcggtgtat ttgtcgcacc agaaaatact ccgataaagc
     1561 ccaatcctgc aagaaaagga acagaaacat ggagcaaagg cgctagtgct gcaccaagcg
     1621 ttgcaccaat ttcaaacaat ggtgtcactt ctcccccttg atatcccgcg cctaaagaca
     1681 aaactgtgaa gaataacttc ccaataaaat catagacatg tgcctgtcca ttaaatgatt
     1741 cttgaagtaa cggaagactc aatccgagat aacgttgtgt ttgcaaaatc aatgctgctg
     1801 ccacaactac agctccacct atcgtatttc gacaaacagg atgaggaatc cattgtgcat
     1861 atcgcgcttt taaaaagacg atcgaacggc tgaataccca accaatcaaa ccaaaagcaa
     1921 tcccagcaac aaaaactttg aagaacagca acatactcca ttcaggaatc acacccattt
     1981 ggtaatgtag atgagaaaca ccatagcttt ctgtcatgaa atttgcaaac agtgcagcaa
     2041 agaagctagg gaagatcgct tctgttcgga ctttcccgat tgccaacact tctaatccaa
     2101 ataatgttcc agctaaaggt gtgccaaata cagaactaaa acccgcactg attccactga
     2161 taatcaagat ttcccgttct gatttatcca atcggaacaa acgtgccgta ttatctgcta
     2221 acgctccccc catttggact gctgttcctt cacggccaac agatccccca aataaatggg
     2281 tagttatggt tccaaacagt gtaagtggaa taagccgcaa cgggattttt tcttctccac
     2341 catttccttg atcgatcact aagttgtttc cacgactaga aagtccgccg tgatacgcat
     2401 agagataagc aaaaagcgca ccactaactg gtagtagaca aaggagccaa ggatgtgata
     2461 gtcggatatc tgtcacaact gtcaggctat ttaagaaaaa agcagacaag ctccccatga
     2521 atataccgag aatcgctgtg atcaccagcc acttcagcat atacactaaa atgcgtactg
     2581 gactcggtaa ttgtttttcc ataaaaaatt cctcctcaat aaaaaaacgc ctactaaaga
     2641 aaggggctgt ccccagactt tagtaggcgt cattagctat ctttcgttag catttaaagg
     2701 cgaacaccat cacctattca cttccattaa aagatagcat atttttctgt aaaaaacaat
     2761 aaaattatcg ttctttcttt acacgttcag caaattcata gaaaggagcc aatttcgtta
     2821 actgttgatt acattggtca ctatcatgac aaatataatt cccctttttc gtatacgtgc
     2881 catcagaacc tgctttcgtt gtagaaagaa aaagcgaaac attcgatgtt ttctgacaga
     2941 ttgcacagat tcccttattt atcgttggtg ataaagtacc gctaatccct tttaattttt
     3001 catcttcgta atagaccatg aattttttct gcgtacctgg atcattccag ccgatgtacg
     3061 tatgttcttg caagtcgatt tcattccaag ctggttgttt gagttttttc acttttcgga
     3121 ataatttctc gatttgtggt ttgctaggct gcataaacgg ctgaacgatt tgcttcagct
     3181 cctccaatgc tctttctgct tgcgcttttg tcagttttac gtctaaaact gtcgttaaaa
     3241 atgtatcgat tctggcagtt cgtgatctaa tatgtctcga atcttttcag ctgctagtgc
     3301 ctgaactgtt tgtaccgttt ttggatcatt gacagatgag tacgcattta ataactgttc
     3361 tacttctctt attataaaaa aatattggta tggttttatt gtttgttcca tgattttaaa
     3421 gtcctttctt ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg
     3481 tgttacctga agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc
     3541 cctttaatgc ttattattct atgtacatga ttcattcgat aaataaaagt aaaccgtatc
     3601 ttgaacagat agtcaactgc aaacagaaaa aaggactgtg acaaa
//
LOCUS       DAFEHS010000132         3424 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFEHS010000132
VERSION     DAFEHS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.139"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(109..564)
                     /locus_tag="LP327_002693"
     CDS             complement(109..564)
                     /locus_tag="LP327_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726758.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="LP327_002694"
     CDS             complement(718..1926)
                     /locus_tag="LP327_002694"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBL1726759.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="LP327_002695"
     CDS             complement(2038..2370)
                     /locus_tag="LP327_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL1726760.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="LP327_002696"
     CDS             2518..3393
                     /locus_tag="LP327_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL1726761.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
BASE COUNT          944 a          708 c          612 g         1160 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaag
//
LOCUS       DAFEHS010000133         3294 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFEHS010000133
VERSION     DAFEHS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.140"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            39..590
                     /locus_tag="LP327_002697"
     CDS             39..590
                     /locus_tag="LP327_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HBL1726762.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1266..1520
                     /locus_tag="LP327_002698"
                     /pseudo
     CDS             1266..1520
                     /locus_tag="LP327_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1590..2102
                     /locus_tag="LP327_002699"
     CDS             1590..2102
                     /locus_tag="LP327_002699"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL1726763.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2099..3136
                     /locus_tag="LP327_002700"
     CDS             2099..3136
                     /locus_tag="LP327_002700"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL1726764.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1214 a          555 c          654 g          871 t
ORIGIN      
        1 agagcgagca agacaggaac gagagaacaa acatactcat gaaaagacta gaggcttcga
       61 cctcgaactt taataataag ttagatgttt ttgaagaaaa gactaagcat gcaagattaa
      121 actttgaaac aactgcaaag cactatatta aacgacttga tgaagataat ttgaaattag
      181 attttcaaca agctattcaa gatgaattaa gcgatactaa agacgaaata cgtgaagtga
      241 cgaaacaagc gagagaagaa acaaaagagt ataaagaaat cttagaaagt aaaataaaag
      301 accataacaa agtggttgat aaatcgaata cagcgctaaa agtgatgaca aaaggcgtta
      361 caaatatatt ttttgtgcta attatttttg tacttgtaat gcttgtgaca ggtccgatag
      421 gtcatttctt tggtattgaa catttatata gctttattaa tggttttatt gatgaccacg
      481 aaagtgcatg gcgatatttg atgttgatac tttatgcagt accctatgta ttcttcgcta
      541 tcattctgtg gctcatagca ttgtttttta gagtatttaa tgacctttag gtagatttat
      601 atataaaaag ggctaaatac gcaaaatacc cccttaaaat gcaaaataaa tgcaaataag
      661 ggggtaactc cttatgaaaa aacaactaat agtattataa cacgttttta tgccgagaaa
      721 acttattggt tggaatgggc tatgtgttag ctaacttgtt agcgagttgg ttggacttga
      781 attgggatta atcccaagaa agtaccaact caacaacaca taaagccctg taggttccga
      841 ccaataagga aattggaata aagcaacgaa aggagttgag taaaatgagt gagaaaacga
      901 agaaaatcat tcgagtatca ttagcttttt cacaagtagt agtgcaatca ttgatagttt
      961 attatactta ccagcaatat aaattatcta aagagaatta agttttgatt atgtaataag
     1021 ttttggtact aaaaaagact tgatctaatt agaccaagtc ttttgttagt gttatattaa
     1081 taacaaaata aaaataagaa gtcgctcact ccccgaccaa agtttgtgag caaaataaac
     1141 agtcgatgtt tgtttcttaa ttgtatatct tgatattaaa cgatttaaga ttattcttca
     1201 agatatatat tcgcaaatgt aattggaaaa gcgacttctt aaacaaaatt aaggagtcgc
     1261 tttttatggc aaaaataacg aaaaatgagc aagaaaattt ggtggaaaat tacacaaaaa
     1321 aaccggcgta ctctaatagc cggttagacg cacatttcgt gtgcacttct aatccgaaat
     1381 tgagctttga cgctatgacg atagtcggaa accttaataa aaacagcgct aaaaagctat
     1441 ccgattttat gagtttagat ccacaaatta gactttggga tatacttcaa acaaaattta
     1501 aagctaaagc actacaagac cccctatgtt aggctagttg tcgtatagga taataagtta
     1561 taataaagac tatacggagg atgattacca tgacaaataa aaacaatgaa cgttactcta
     1621 ccgaactgaa agcgacagcc atcaagaaaa tgatgccacc cgaaaatagg gggattcgcc
     1681 aattaagtca aactttgggc attccagaag cgactctttg ttcttggcgt aaaaaagcta
     1741 gaattcaagg gaatactaca cccggaacga aaaaaaatgt cgaaaaatgg tcaagtgaag
     1801 ataaattttt aattgtgatg gaatcattct caatgaacca agtagaactt gcggaatatt
     1861 gccgaaagaa gggcttattt aaagaacaaa ttgaggcttg gaagaaaacg tgcttatctg
     1921 cgaatgaaca ggaagaaaat cgaacgagag aacttgcaac tgaactaaaa gaagagaaaa
     1981 aacaagcgcg tcaattagag aaagaattac gtcataagga aaaagcatta gcggaagccg
     2041 ctgctctctt attactaaga aaaaaagcac acgcgatttg gggggaccaa gaggaagaat
     2101 gatttcatct tcagatcgca aacaagctgt tgaacttatt caagaagcca atcgcaatgg
     2161 ggcccgtctc acctatgcct gtaatgagct gaatattagc gttcgaacct acgaacgatg
     2221 gacgcgtgaa ggtacgatag cgcatgatca acgccctttg gcgaaacgtc ctgtacccaa
     2281 aaataaactg acggatcaag aacgcgaaaa aataatagaa accgtttcaa aaaaagaatt
     2341 tatgaatttg ccacctagcc aaattgtacc aaaacttgcg gattgctcga tttatattgc
     2401 atcagagtct agcttctatc gtgtattgcg agaaaaaaat atgcaacatc accgtggccg
     2461 aagtcaagtt tcacaaaaac gcataccgcc aagtcacctt gcgacgaagc caaatgaagt
     2521 ttggacttgg gatattactt ggctaaaagg tccaataaaa ggactctttt accggttata
     2581 tctcatcatt gatttattta gccgtaaaat ggtaggctgg gagatttggg aatcagaaga
     2641 agctcgtcat gccgaaaaac ttattaaaca gacggtgctt aaggaacgta tccaaggaca
     2701 gccgttggtt ttacactccg ataatggcag ccctatgaaa gccgcgactt ttcaagtctt
     2761 actcgaaaaa ttaggaattc aaagttctta ttcaagacca cgagtgagta atgacaatcc
     2821 ttattcggaa gcggctttcc gaacgttaaa atatcgacca gaatatccca ataacggctt
     2881 taaaacaata gatgatgctc gaaaatggac gaaagagttt gtcgattggt ataacgattc
     2941 tcatttgcat agtgggattc aatttgtgtc tcctgcagcg tgtcatgaag gacgacatct
     3001 cgctatttta gagaaacgaa agcaggttta cgaggacgct aaaaagcgcc atcctgaacg
     3061 ctggtcaaaa ggtgttcgaa attggactcc acatcaatcg gtggcgttga atcctttaaa
     3121 agaaacagaa agttaacagg agcataatct ttcaagatta tcaaataacc ctcgtcaccc
     3181 aataggggaa aaagttattc ttcccctatt ggataacgag gccaaccgac catagggagg
     3241 ttctgctttt tgataggtgg aaaataaaaa tacgacaact atattgacaa acac
//
LOCUS       DAFEHS010000134         3192 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFEHS010000134
VERSION     DAFEHS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.141"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(45..1085)
                     /locus_tag="LP327_002701"
     CDS             complement(45..1085)
                     /locus_tag="LP327_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL1726765.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1362..3164)
                     /locus_tag="LP327_002702"
     CDS             complement(1362..3164)
                     /locus_tag="LP327_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1726766.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
BASE COUNT          955 a          667 c          549 g         1021 t
ORIGIN      
        1 taaaaagggg atgtgacact gtcacactcc ctttttaatt tattctattt ctttatattt
       61 attttgtatt ccgttacaaa ctcgcgtact tggttaggtt ccagagtgac atctccaaaa
      121 ctttcaagtc gctccgctcc tggcagttct tgagtctcaa atgtgatacc gccatgattt
      181 actaatttat tcccgcgcat ttccggtcca tcttctccaa aatttgctgt aaaaatcacg
      241 attcctgaag catctgtttt tacttggatc gatacttttt catctgggct tgtcaattcc
      301 gcagcattta gtaaaccaga ttgttttttc aacaaaaatg gatgatcgat accgcccact
      361 atcattgatt ggcgaaaatc actggcaaag acctcaccta attttttagg acgttggaag
      421 tcaaatgctg tgccacttac tgaaactact tctcctgtag gcgttgtatc tttattgagc
      481 gtagcaaata atcggctatt caaccaaagt atatgttcgt cgattgactg tgctggatca
      541 ccggttaaat tgaaatagac atgatttgta ggattgaata ctgttttgcg gtcactgatc
      601 ccttggaccg tcactcgcca tgtatcatcg ttcgtcagcg tataccgtac ttctacatct
      661 aaagttccag gaaatccgtt ttccatgtct gggctagtct gatagaaaat gaccgatgct
      721 tcgttttcac cattgatgac tgtataatgc cactgcttgg cttcaaagct aggtgcacca
      781 ccatgtaaac aatgaccagt gtccgggtct gtttttactt cgaacgtttc atcatctaat
      841 ttgaatgtgc cattcgtaat ccgtccagcc atacgtccga ttgaagcacc gatataaggg
      901 tctttttcta gatattcttt tgcttcatca aaacctaaga tcaactctcg tttttcatta
      961 tcgattattg tttgaaaatg aacaacccgt gccccaagat cactgacaga caaagatgtc
     1021 ccattttgat tcgtgatcgt aatcaagtga tagttttcgc caaaattttg ttcattgatt
     1081 tccatgttcc ttgcaccctc ttttctttca tttgcactga ttccagtata catggaaacc
     1141 gttttcttaa cagcttattc cgtgagtaac gagaaaaaat ttactatatt ctaataaaag
     1201 accgtgaaaa aaaggttgtg acaaaaatat tgtcacaacc tctttttccg aacaaacggt
     1261 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatagg aggagctatt
     1321 tttgcttggc tgttcttatt acttgatgca ggacagcttt ttcacaatct ctttttcaat
     1381 acgattccag tatataacca tgtcgtcagt acatagtaga tgaaatacaa tacgaagaag
     1441 atcgtaaacg gaacccaaat atctttgtat ggattttgca tgaatagacc aaacatttgt
     1501 aatccgaata agacatggac tacacctaag attcctggtg ctaagaataa cacgcctact
     1561 tctctacgga tcgattgttt aagcaacccg cgtcgtgttc ctatcttttc aagcatttca
     1621 taacgagcaa tgtcactctt gcttcctgac aagatcttga acatcaaaca gctagcaagc
     1681 atggtcaaga aagctaaacc taggaagaat cccataaatt caaaaccaga gaatagacca
     1741 ttatacactt catacatttc atatttctgg ttgctgtact ctactttttg caaagatgga
     1801 ttgttcattt tattttgagc aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct
     1861 tttacctgaa ctagctgtaa agtggattgt ggcaagttca gttgatcaaa ttctgattga
     1921 ctgactacct tgatttcttt ttcttgctgt tccggcaatt catagcttcg aagttcctct
     1981 tgtacagtca gttcattcat tttttcagca ggaacttttt ctttcgtgat cttcggcggt
     2041 gttccatctt catgttttat catgatcaat ggttgagccg caaattcact tgcattatag
     2101 taaatcgttc ccgcatcttc tttttgagta taagaagcag aaagtgttgg tgaaagttct
     2161 ttgatttttt gctgatcttc tgcccgagga ttattcatca ctagatcata tccagaattg
     2221 ttttctgtca agtctgcaat ttcattttta aaacctaatc ctaccgtcaa agcgcccagt
     2281 gccaaggcaa acagcatagc gaccattgag agcatttgtg tatattcacg aatacgaaag
     2341 tttaactgtg acaatgtgaa attgttcaat tttttcaatg aaatctgttc tgatttcttt
     2401 agtaatgata aaaagaaaat gatcacagaa tggaaaatga aataagttcc taatacgatc
     2461 gtaactaagg ctactcctat accaaagact tgataggtcc ccacgtgttt catcatatag
     2521 taaccgcagc ctagaaaagc aattcccagt actacttcaa ggaaaaacaa gaatttatgc
     2581 tgcttgattc ttgttggtgt ctgatccgca cgaatcaatg ctaatatcgg ttttttgaca
     2641 atcgaaaaag cattgacaac agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg
     2701 atcaacaacc cgtgaatact aaaaggtgta aaatgcgaaa tattgagatc taactgatcc
     2761 actaataatc ggtggacgat acttgtcaat cctacaccga cagctgaacc cactaaagta
     2821 gctgcgatcc ctactaagaa tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt
     2881 gctcctagca tcataaacat tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt
     2941 gcatataaaa tataaacgaa tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg
     3001 ataactgtag ccgcaacgat cgtattactt ttcaaaaaag cttcgttgct tgccattgat
     3061 tcaaacatgt aaaaaatagc agatgccata actaatcctg agaataacac caggtagtct
     3121 cttaaacggc tcttgatccc agtaagtgat aatttccaaa gcatagtcaa tctcccccta
     3181 ctgttccaag tt
//
LOCUS       DAFEHS010000135         3174 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFEHS010000135
VERSION     DAFEHS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.142"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            118..1740
                     /locus_tag="LP327_002703"
     CDS             118..1740
                     /locus_tag="LP327_002703"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL1726767.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEEEVIPLDSVSKSIQETKAELAQAETELF
                     NMLKELNGTTEEAENELQAFISQLMTDGDNNE"
     gene            1721..2269
                     /locus_tag="LP327_002704"
     CDS             1721..2269
                     /locus_tag="LP327_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL1726768.1"
                     /translation="MVIIMNKERAIPKVRFKSFSGDWKKLKLSSVTSRVRGNDGRMSL
                     PTLTISARNGWLDQRERFSGNIAGKEQKNYTLLRKGELSYNKGNSKLAKYGVVFMLDN
                     FEEALVPRVYHSFKTTNEASSKYIEYLFETKKPNKELRKLITSGARMDGLLNINYDDF
                     MGIKITIPKIKEQKKLDPYSNK"
     gene            2332..2985
                     /locus_tag="LP327_002705"
     CDS             2332..2985
                     /locus_tag="LP327_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL1726769.1"
                     /translation="MQQLFPQKGESVPKIRFTGFSDDWELKELKEFIGEDVSDGDWIQ
                     KEHIHESGEYRIVQTGNIGIGRYIDKPESAKYLNQESFDELKANEINPGDILISRLAD
                     PAGRALILPFTSSKMVTAVDVAIIRPNKNFISHFLVTRMNSSETLNDISKQVSGTSHK
                     RLSRKNLEKIELNVPNIEEQEKIGQLFKKLDEAIAGHEQKLATYQELKKALLQRMFV"
     gene            3007..>3174
                     /locus_tag="LP327_002706"
     CDS             3007..>3174
                     /locus_tag="LP327_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726770.1"
                     /translation="MAITFTSESELEQTLIHQLIGGESQWTYRGDLNTEDKLWDNLRE
                     KLERNNKDVLND"
BASE COUNT         1161 a          543 c          624 g          846 t
ORIGIN      
        1 aaatgtagct tatccatttc attaaaatac atatggtgaa aaaacaagtt caaatagtaa
       61 ctaatttttt aaaaaactga tttataaaaa atacaacacg aatgaggaaa ataaccaatg
      121 acagaacaaa tcgaaaaaaa ttcaaaaagc ttatatcagg cactgtggaa ttccgcagat
      181 atcctgcgtt caaaaatgga cgctaatgaa tacaagagct atttactagg cttggtcttt
      241 tataaatact tatccgacaa catgctgcgg tatgtctccg ttctattgga ggaagaaacg
      301 gacgacctaa ataaagcaca agacctttac gttgaagcat ttaaagatgc agatataaag
      361 gatgatctct tgtatgaact aaaagatgag ttttcatata ccatcgcacc cgcattgacc
      421 tttaccgcgc aagtggcagc cattcatgat ggatcttttc aattagaaga cctggtacaa
      481 ggtttccgtg acattgaaca atccagcgaa aactttgaga acttatttga agacattgat
      541 ctgtattcta aaaaactggg gtcaacccca caaaaacaaa ataaaaccat cgcagatgtt
      601 atgaaagaat tagaagggtt aaacatggcg gggcatgctg gcgatgtctt aggcgatgcc
      661 tatgagtact taattggcca attcgcatct gaatccggta aaaaagccgg tgaattctat
      721 accccacaac cggtcgcaaa attgatgaca caaattgttt tacaaggaaa agaagataaa
      781 aaaggctttt ctgtttacga cgcgaccatg ggttcgggct cgttactttt gaacgctaaa
      841 aaatattccc atcaaccggg aaccatttct tatttcggac aagagttgaa tacgtctacc
      901 tataatttgg cgcgtatgaa catgattttg catggggttc ccattgccaa ccaacacttg
      961 cataatgcgg ataccttgga tcaagactgg ccaacagaag agccaacgaa cttcgatggg
     1021 gtgctcatga acccaccgta ttctgcaaaa tggtcggctg ataaaggctt cttagatgac
     1081 ccacgcttct ctgcctatgg cgtattagcc cctaaatcaa aagcggactt tgctttctta
     1141 ttacatggct actatcattt gaaagatacg ggcgtcatgg ccattgtttt gccacatggg
     1201 gtgctcttta gagggggcgc agaagggaag atacgcaaag ccttgttaga aaatggtgcc
     1261 attgacaccg tcattggctt acctgctaac atcttcttta atacgtccat tccaacgacc
     1321 gtaattatct tgaagaaaga ccgtgcgaaa aaagatgtgc tctttattga tgcgtcacaa
     1381 gattttgaaa aaataaaaac gcaaaatacg ctacgagatt accatattga cgctatttta
     1441 gaggcctata aaacccgtac aattaaagac aagtatgctt atgtcgcaga atttgatgaa
     1501 attgtggaga atgattacaa cttaaacatt ccccgttacg tggatacctt tgaagaagaa
     1561 gaagtgattc cccttgactc tgtttcgaag tctattcaag agactaaagc agagttagca
     1621 caagcagaaa ctgagttatt taatatgttg aaagaattaa acgggacaac tgaagaggca
     1681 gagaatgagt tacaagcgtt tatttcacaa ttgatgactg atggtgataa taatgaataa
     1741 agaaagggca atacctaaag ttcgttttaa aagctttagt ggagactgga aaaaattaaa
     1801 attaagttcg gttactagta gagtccgagg aaatgatgga agaatgagct taccaacatt
     1861 aacgatatct gctagaaatg gatggttaga tcaaagagaa cgtttttcag gtaatattgc
     1921 aggaaaggag caaaaaaatt atacgcttct acgtaaaggt gaactatctt ataataaagg
     1981 gaattcaaaa ctagctaaat atggagtcgt atttatgtta gataattttg aagaagcgct
     2041 tgttccaagg gtctatcata gttttaaaac tacaaatgaa gcatcatcaa aatatatcga
     2101 atatttattt gaaactaaaa agccaaataa ggaattaaga aaacttataa catctggagc
     2161 tagaatggat ggattattaa atataaatta tgatgacttt atggggatta aaataactat
     2221 tcctaaaatt aaagaacaaa aaaaattgga tccttattca aacaaataga tggcactatt
     2281 acccttcaac agcaactcct aaccgactac aagcaattta aaaaagcgtt gttgcaacag
     2341 ctattcccac aaaaaggaga atcagtaccg aaaattcgat ttactgggtt tagtgatgat
     2401 tgggaattga aagaattaaa agaattcatt ggtgaagatg tatctgatgg tgattggatt
     2461 caaaaggaac acatacatga atcaggagag tatagaatag ttcaaactgg aaatataggg
     2521 ataggaagat acatagataa gcctgagagt gcaaaatacc tcaaccaaga aagttttgat
     2581 gagttaaaag caaatgaaat aaaccctggt gatattttaa tatcccgatt agctgatcca
     2641 gcaggaagag cacttattct gccatttact tcttcaaaaa tggtgactgc agtggacgtt
     2701 gctattatta gaccaaataa aaatttcatt tctcattttc ttgtcactag aatgaattca
     2761 tcagaaacac taaatgatat ttcgaaacaa gtaagtggta cctcacataa aagattaagt
     2821 aggaaaaatt tagaaaaaat agaacttaat gtacctaata ttgaagaaca agaaaaaatc
     2881 ggtcaactct tcaaaaaact ggacgaagcc atcgcagggc atgaacagaa actcgccacc
     2941 tatcaagaac tcaagaaagc tctattacaa cggatgtttg tttaaagtaa agagaggagg
     3001 aacatcatgg cgattacctt tacatctgaa tcggaattag aacaaaccct catccatcag
     3061 ttgattgggg gggaatcaca atggacctat cgcggggatt tgaataccga agacaagctc
     3121 tgggataacc tgcgtgaaaa attggaacgc aataataaag atgtcttaaa tgac
//
LOCUS       DAFEHS010000136         3074 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFEHS010000136
VERSION     DAFEHS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.143"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            112..777
                     /locus_tag="LP327_002707"
                     /pseudo
     CDS             112..777
                     /locus_tag="LP327_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            813..1058
                     /locus_tag="LP327_002708"
     CDS             813..1058
                     /locus_tag="LP327_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL1726771.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1161..2030
                     /locus_tag="LP327_002709"
     CDS             1161..2030
                     /locus_tag="LP327_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL1726772.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2011..2745
                     /locus_tag="LP327_002710"
     CDS             2011..2745
                     /locus_tag="LP327_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL1726773.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2778..>3074
                     /locus_tag="LP327_002711"
     CDS             2778..>3074
                     /locus_tag="LP327_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBL1726774.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKI"
BASE COUNT         1072 a          431 c          633 g          938 t
ORIGIN      
        1 atttagatag tagtggaaag acctctattt atcttttgaa agtgaataca aaaataaagc
       61 acactctgct ataatatagg tgattaatcc atttgagaga ggtgctttat catgtatcaa
      121 aattatacca taaaccaact ttgtttgcct atagatcttg aaattaaatt agaagaaaat
      181 gattttgctc atgccattgt gcagtttgtt gactctattc ccgatgaagt ctttcttcct
      241 tattatcaat ctatgggtag accccaatat catcctcgta tgctactttc cattattctt
      301 tgtgcctata ttcaaggggt ttattcgggc cgccaaatac aaaatatgct gatagatagt
      361 attcggatgc gctacttatc tcaagagcaa ttccctaact ttcgtactat taatcgtttt
      421 cgcgttcatc cgattatgaa cgatattcta gatcattcat ttgttcaatt tcgtgagcta
      481 ctggttcaaa gtggcttaat cagtggcagt gctctataca ttgatggtac aaaaattgaa
      541 gcggatgcga ataaatactc tttcgtttgg aaaaaaagta ttttaaagta taaggatagc
      601 cttgatcaaa aagcccttga aaactaccaa caaatggtgg aaacaaaaat cttacctgag
      661 ttaatagatg atttaaagga tgaactttct atagaagaga ttgaaaggat tcgtcaatct
      721 ttggaagaaa aggaaaatca attaattgaa gcgattgacc aaaccgaaac agtagaaggg
      781 ggctgaccca ttattaataa ggagtggaca acatgattga tgtaaatgag ctattggaag
      841 aagctattag agaaactgaa aatctaaatg atggcgaagt ttttcttgtt aaagatttat
      901 tcaagggata tgtggggaat aggataccca gaaaggatcg acttctgctt ggaacgttat
      961 ttttaaacca tgttagcaaa atgaatggta atttaaaggc tattgaaaag acttcatcta
     1021 atcagcagag gtataaaaag acaattgata agttttaact atcttttgtg aaattaaaaa
     1081 aattataaat atgttgagta gaagcaaaag atttggcttg gaatttttac agcagtgaat
     1141 cgataaaagg agcatgcttg atggtagata atattattaa atcagtagca gagaaattat
     1201 cctctctgtc ttatatagaa ggtattgttt taggtggttc acgtgcaagg ggcacccata
     1261 cagaggattc ggatatagat atcggcatct attacaattc agaatcattt gacataaata
     1321 ctattaatca attcgctaca aagctggatg atgagcatag aaataacctt gttgtacctc
     1381 ccggagcatg gggtgattgg attaatggcg gcggatggtt agtcataaac gggtatcatg
     1441 tggatttaat tttacgtgat attaaacgtg tggaacaaat aatgaaagat acagagcacg
     1501 gaattgttac tgccaattat cagactgggc atccccatgg ttatattagt gcaatgtatc
     1561 gaggagaatt agcgattagc aaaatactat atgctaagaa tgaaagctta tgcgaattaa
     1621 aaaaacaggc agaaacttat cccaatgctt tgcagaaaag tttagttaac ttttttatgt
     1681 ttgaagcagg gttctcttta atgtttgtaa aagcaaattc gggaacagac gataaatatt
     1741 atattgcggg tcatgttttt cgtatagttt catgtttaaa tcaagtgtta tttgcatgta
     1801 ataatgctta ttgtatcaac gaaaagaaag ctataaaact gcttgaaact tttgaacata
     1861 aacctgaaaa atataccgag aaggtaaatc atatttttga agtactcggt atctcacttt
     1921 ttgaatgcta cgacatgacc gagaagcttt ataatgaagt gaatgaaatt gtatcggaga
     1981 taaataactt tttaaacgag gagagttcag atgaaagaaa acaaatatga tgataatata
     2041 ttttttcaaa aatacagtca aatgagtcgc tcgcagaaag gactggctgg tgcgggagaa
     2101 tgggagactt tgaaaaagat gctacctgat tttaagggta agcgtgtgct tgatttagga
     2161 tgcggctatg gatggcactg tatatatgcg atggaaaacg gtgcttcctc tgtagtaggt
     2221 gttgatattt ctcataaaat gctcgaagta gcaaaaggaa aaacccattt tccacagatt
     2281 gaatatgaat gctgtgccat agaagatgtg gatttcccag aggagagctt tgatgtaata
     2341 ctaagttcgc ttgcgtttca ttatgtagca gactatgaga atttaataaa aaagatatat
     2401 aggatgctga aggctggtgg caatttagtt tttacagttg aacatcctgt ttttactgct
     2461 catggaacac aagactggta ttataacgaa aaaggagaaa tactgcattt cccggtggac
     2521 aattattatt atgagggcaa acggacagct atgtttttgg aagaaaaggt tacaaaatat
     2581 catagaacac tgaccacata tctaaataca ctgctttcaa atagttttat aataaatcag
     2641 attgtggagc cacagccgcc agagaacatg atggatattc cggggatggc ggatgaaatg
     2701 cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga tgtaataata tagaaaaaat
     2761 aaacgaggag tatgtaaatg agatcagaaa aagaaatgat ggatttagta ctttctttag
     2821 cagaacagga tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca aatattaata
     2881 tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat atagaaccgt
     2941 ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg caaaagccgg
     3001 aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg ctatttgatg
     3061 attacaataa aatt
//
LOCUS       DAFEHS010000137         3070 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFEHS010000137
VERSION     DAFEHS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.144"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(334..696)
                     /locus_tag="LP327_002712"
     CDS             complement(334..696)
                     /locus_tag="LP327_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726775.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="LP327_002713"
     CDS             complement(770..1150)
                     /locus_tag="LP327_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBL1726776.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="LP327_002714"
     CDS             1214..2407
                     /locus_tag="LP327_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL1726777.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="LP327_002715"
     CDS             complement(2484..3068)
                     /locus_tag="LP327_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL1726778.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          958 a          587 c          504 g         1021 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat
//
LOCUS       DAFEHS010000138         3065 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFEHS010000138
VERSION     DAFEHS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.145"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            7..624
                     /locus_tag="LP327_002716"
     CDS             7..624
                     /locus_tag="LP327_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726779.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDTLKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1949..2503
                     /locus_tag="LP327_002717"
     CDS             1949..2503
                     /locus_tag="LP327_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL1726780.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            2757..>3065
                     /locus_tag="LP327_002718"
     CDS             2757..>3065
                     /locus_tag="LP327_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726781.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYVLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT         1195 a          346 c          494 g         1030 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatac attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaataaaa atatagaaat aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aagaactagt agaagaagga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaattatga acttgcgtaa agttataaaa
     1561 tattaataaa aatattttat taatgaaaag cttggtagca cggttattca tatgctaatt
     1621 tatttcactt tatagtaagg ggagttctta gtatattgtt caaacttaaa tttcatataa
     1681 tttaattatg taaattagct attctaagaa cttctacgga taactgtccg tctaaaattt
     1741 aaataagagc ttagaatctt catataagta aggttaattt ttttgtacaa aaaagtcgga
     1801 atattctgag ttactgaaat gatataaaat cataaactaa accccttgat tttcttcact
     1861 tttaacagag tatatatata agttcatgat tatggtaaaa tatatatata atcatgaact
     1921 cagaatattt ttaagaaggg aaacaatagt gagctataat gtacaaccgt taaggacaaa
     1981 tgaagagata caagattttc agttttggct aagaagaaca agtaatccag aaagagatag
     2041 ttttttattt ttgtttggaa tcaataatgg gttacgtatg tcagatatta taggattaaa
     2101 agttggagac atacgaggta aaagtaagcc tattattgtt gaacataaaa caggtaagcg
     2161 aaaaccgatt tttatagata atttgagaga agagatttta ttatatactg aaggtaaaga
     2221 agaaaatgat tggttatttc caagtcggca acaaggtcga catataacaa gggatcgtgt
     2281 ctatcagata tatgcagata tagcagaaaa attaggaaga gatgatatag gaacacatac
     2341 attaagaaaa acttttgggt atcactacta caagaaaact agagacattg ctaccttaat
     2401 gtttattttt aatcatagct cgcaagcaat tacaaaaagg tacattggta taactgaaga
     2461 tgaaataggg gcatcgttac gtggttttaa attgggaggc taattgagat attaggatat
     2521 attttttgtg tataatgaaa acttagaagg tgagattaat gaagataact gaattttcgg
     2581 aagtatttaa aacgtttagt aacatttaca atagaatacc agagatagaa caaaatgttg
     2641 tagttgataa tgattattat atagagaatg attttaataa accaagaaaa tatgacaacc
     2701 tatacaattt tatacacaat agaacaacaa taaataacaa cagtaacact cgagttttga
     2761 ttaattcagc atattttcat attaaatatt taaagaaata tgagtatgaa gaagttgtat
     2821 tagattatgt gctaaataga cagtcatttt taatttatgc aataaataac ggaaatttgc
     2881 caagcaataa aaagaacata attattgaaa catatgccta tctggagggg ggatctaaaa
     2941 aactaattaa gtcatgtaat atagagtatc aattatcagt aggagaaatt aaatgtgttt
     3001 tcgaagaaga tttagaactt gacttaaaaa aaatttttgc cagcattcat gattatatag
     3061 gtttg
//
LOCUS       DAFEHS010000139         3046 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFEHS010000139
VERSION     DAFEHS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.146"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(55..636)
                     /locus_tag="LP327_002719"
     CDS             complement(55..636)
                     /locus_tag="LP327_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBL1726782.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(642..1901)
                     /locus_tag="LP327_002720"
     CDS             complement(642..1901)
                     /locus_tag="LP327_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL1726783.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1903..2949)
                     /locus_tag="LP327_002721"
     CDS             complement(1903..2949)
                     /locus_tag="LP327_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726784.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT          862 a          629 c          491 g         1064 t
ORIGIN      
        1 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
       61 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
      121 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
      181 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
      241 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
      301 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
      361 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
      421 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
      481 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
      541 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
      601 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
      661 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
      721 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
      781 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
      841 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
      901 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
      961 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     1021 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     1081 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     1141 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     1201 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     1261 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     1321 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     1381 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     1441 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     1501 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     1561 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     1621 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     1681 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     1741 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     1801 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     1861 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     1921 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     1981 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     2041 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     2101 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     2161 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     2221 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     2281 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     2341 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     2401 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     2461 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     2521 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     2581 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     2641 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     2701 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     2761 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     2821 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     2881 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     2941 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     3001 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaa
//
LOCUS       DAFEHS010000140         2719 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFEHS010000140
VERSION     DAFEHS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.147"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="LP327_002722"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="LP327_002722"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL1726785.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DAFEHS010000141         2492 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFEHS010000141
VERSION     DAFEHS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.148"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..305
                     /locus_tag="LP327_002723"
     CDS             <1..305
                     /locus_tag="LP327_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL1726786.1"
                     /translation="ITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGIL
                     RRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(1171..1701)
                     /locus_tag="LP327_002724"
     CDS             complement(1171..1701)
                     /locus_tag="LP327_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL1726787.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1698..2315)
                     /locus_tag="LP327_002725"
     CDS             complement(1698..2315)
                     /locus_tag="LP327_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL1726788.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          824 a          473 c          290 g          905 t
ORIGIN      
        1 ctattacgtt tgactgtgga aaagaatttt ctaactggaa agccattagt aaccaacatg
       61 atattgatat atattttgcg gaccctggaa caccttctca acgcccatta aacgaaaatt
      121 ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat agattttaga gaagtgaatc
      181 agacatttat ttccagtgtc agcaatcaac gcaatcatat tccaagaaaa tcattgaatt
      241 acagaacacc aatcgaaata tttttgagct atgtacaaga agcattttat tctagcttaa
      301 tttgacaaat catataattt aaaattaaca ttgattctcc tatgaaaata tcttttacct
      361 ttctcctgtg aaaaggaaat ttcctttttt atagtgttat tgatataatc agaatattca
      421 tcaaactctg tatctggagt taaaaatcta cagtaacatc tataagcttt gctagtaatc
      481 ttcttgggtt ctctctccat tgtcaagcaa catcaaaaca cctacttact attcgattct
      541 tttctgccaa aaacgagtca gcatcactca caacttatgt gttgctgact ccgactagtc
      601 ctttgttcaa tatcaactta ttaaacagat ataatatttt tctttatttc gtatactcca
      661 agacttccat ctttctttca tcttatctct gaaaaaaaca cagcaaagtg cccttaataa
      721 atatacatta gaatttgcaa attttttcat cttaagatat atcctattca tattctttac
      781 aatcctaatg ggtgattaca aatctattta gttcaagttc tacattataa acacctttct
      841 tgatagtatc atctgctgca caagcgatgt cgatccactt attaggggat ttacgtgctc
      901 aaaagtggct attccttttg ggaagtccat ccataataat ggtccatgga tttttttagg
      961 gtcttgtcgt tcaaatttct caaaaacttt attatacttc catagtttta taattatttg
     1021 gtttcacttt ggctcgttca aacaccaact tgtatttctt ttcaacgttc gaactggagc
     1081 cccccctata atctcattat tattttcatg actttttact accgtggaat tacaagcaac
     1141 aacaatatta tctgctaaat gaacaccttt ctatatcacg ctttttgctc caatccaaca
     1201 atgtttacct ataactattt tattattttt attcaaaatc ctatgagtat tcaattcaaa
     1261 aatcgtatgt gaatcggtat ccataaataa tgtatcccac gatactgtac agccatcttt
     1321 gaattgaatt tccttaaagc aaattatact catttttcct gtcgaattaa actttgaccc
     1381 aatatctaaa actgcatttt tccttatgtc aatatgagcc ccttgtgcga taaacgctgg
     1441 cccttgtatt cttagtattc cttcaatact tataatcgtt tcttcatttc ccgcagtatt
     1501 taatactgga aagcccaaat aaactatttt tccagattgt aatactatct caattttccc
     1561 tctcgatttg cgtgtaatcc gtactttacg atgcaccaaa ataggtaatc tccacgcatc
     1621 tgaaatatga agaaatttat aattgaagta tatcgtttta ggtaaattta atatattctt
     1681 tattatcaat ccgagtctca tctttcacca tctctttttg taatcaaaat tagtttataa
     1741 aggattacac ccaatatacc gcccaacgta ttatatacaa aatccgaaat ttgaaacgtt
     1801 cctagcctca aaaaaagttg taaaaattca attattgctg aaaaataaaa tgcaattttt
     1861 cctccatata ctattgagga ccatattttg ggtttcttta tattttcata tttaatagac
     1921 attaccatat aaacatatgg aataaaaaga ataatatttt ctaaacacgc tgtagataac
     1981 tcttttgttc cagttgttgt attatactta taaatccacc agtctcccat aacatcagat
     2041 aaaggattta accaaattgt tcgattgatt aatgttgtaa ataaaatcat cgcagtatag
     2101 aatatcaaag cacaaacttt tctatatttt ctatccttct ttaaatgaag ataaagttcc
     2161 attagtatac gtttatagcc ctcacctgca gcaatgccat ctgttgcata cataaaatag
     2221 accagtccta aaattgtcaa taaaacagca aacccaaagg gttcatataa tttatataat
     2281 atatctgtaa taatttctgc aatcactatt atcataatta tttctccaat atcaagtata
     2341 cacttatcgc ttccaattac acatgatcat attagagtgt attatcacat tctctaatat
     2401 tcaatttatt tttcctaaaa tattcgaaat aatcgtcttg ataacgagta agtattttta
     2461 tacgattgat aacatagtaa caatattttt aa
//
LOCUS       DAFEHS010000142         2491 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFEHS010000142
VERSION     DAFEHS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.149"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            5..1150
                     /locus_tag="LP327_002726"
     CDS             5..1150
                     /locus_tag="LP327_002726"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBL1726789.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="LP327_002727"
     CDS             1321..1779
                     /locus_tag="LP327_002727"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBL1726790.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRYEWSEVEA"
     gene            1938..2408
                     /locus_tag="LP327_002728"
     CDS             1938..2408
                     /locus_tag="LP327_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBL1726791.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          829 a          470 c          468 g          724 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gctatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc a
//
LOCUS       DAFEHS010000143         2398 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFEHS010000143
VERSION     DAFEHS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.150"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            207..2069
                     /locus_tag="LP327_002729"
     CDS             207..2069
                     /locus_tag="LP327_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL1726792.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="LP327_002730"
     CDS             2069..>2398
                     /locus_tag="LP327_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL1726793.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAFEHS010000144         2150 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFEHS010000144
VERSION     DAFEHS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.151"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            78..533
                     /locus_tag="LP327_002731"
     CDS             78..533
                     /locus_tag="LP327_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726794.1"
                     /translation="MICGPANYPTRRKQKEEERIYQLESELYSKNGKHARYIENTRKM
                     FDPVMIDQKIEIDKKRKEKAEEKGWKDFYKEVDHDELAGYGFDVENNRLYLVTHGKPS
                     DDVRALLKKAALRWSPRNKRWQRILTVNAINSVNRNVMNGLGLPQMEEK"
     gene            533..844
                     /locus_tag="LP327_002732"
     CDS             533..844
                     /locus_tag="LP327_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL1726795.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            841..1071
                     /locus_tag="LP327_002733"
     CDS             841..1071
                     /locus_tag="LP327_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726796.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            1078..1281
                     /locus_tag="LP327_002734"
     CDS             1078..1281
                     /locus_tag="LP327_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL1726797.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            1278..1577
                     /locus_tag="LP327_002735"
     CDS             1278..1577
                     /locus_tag="LP327_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726798.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            1567..1731
                     /locus_tag="LP327_002736"
     CDS             1567..1731
                     /locus_tag="LP327_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726799.1"
                     /translation="MKFKIFEEDTRYKLEKELNDFAKNNEIQHISLATSKRGYANYYA
                     AVVSYVSREV"
     gene            1820..>2150
                     /locus_tag="LP327_002737"
     CDS             1820..>2150
                     /locus_tag="LP327_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL1726800.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSE"
BASE COUNT          784 a          319 c          502 g          545 t
ORIGIN      
        1 aacgtctagt gaactataac aaactagttg tcgaattaaa aacaaatatt ctcaaagcga
       61 caaccattcc atcagtgatg atttgcggac cagcaaatta tcctactaga agaaaacaaa
      121 aagaagaaga acggatctat caattagaaa gtgaattata ttcaaaaaat ggtaagcatg
      181 ctcgttatat tgagaatacg agaaagatgt ttgatccagt tatgattgat caaaagattg
      241 aaattgataa aaaacgcaag gaaaaagcag aagagaaagg ttggaaggac ttttacaaag
      301 aagtggatca tgatgaacta gcaggatacg gctttgatgt agaaaacaat cgactttatt
      361 tagttactca tggtaagcca tcggatgatg tacgtgcctt attaaaaaaa gctgcattga
      421 gatggtctcc tagaaataaa cgttggcaaa gaattttaac tgtgaatgct ataaattcag
      481 taaatcgaaa tgttatgaat ggacttggat tgccacagat ggaggagaaa taatggacga
      541 actaatcaca aaagtagagc agtgggctaa agataaagga ctagatcaag cggatccaaa
      601 agcacagttt ttgaaagtag ctgaggaatt cggagaaatt gcttcggcaa tggcaagaag
      661 taatgatgag ctatttaaag atagcgtagg agacgttatc gtcacgctga ttatcctttc
      721 catgcaaaaa gggacaaacg tacaagagtg tttagaaatg gcatacaacg aaatcaaagg
      781 acgcacaggg aaaatggtag atggtgtatt cgtgaagtcg agtgatttgg aggatgtgaa
      841 atgaaatacg aaataccact aagtgaagcg ggcattcaag caattatcaa tggtcgggag
      901 gttaacatag aacttcctga tggtactgaa ttagtcatca gacaaagtta tttgaaagat
      961 atggcagctc cagtattaat tgatcgtttt aacgtgactg attctgtggt agagaaccac
     1021 ttaaaagaat ttcgatcaag tatagacaac actttcagat tagggagttg attgacaatg
     1081 aacaccagac accgcagagt agcaaaacta agaaaacagg aactaaatgt actaaagaca
     1141 aagtttgaaa aagaatatgg aatttcagca gaagaaacat ataaagtggc aagtcagttt
     1201 gttgctgatg caagtgatgc tattcgtaag tttgggattt cgatattaaa tgatgatcgt
     1261 aaatgggagg aaatgagatg aaactaaaag acggatttta cgctagtagt cacggcatcg
     1321 gcggtttaat gctagatatg ccgacaaaga accctaaaac acgtaagaaa ccaaaattca
     1381 aagttggtga catggttcgc tgtgaagcag aagggttcat ctatccgttt cgtggatatg
     1441 tagagcatct ctataatcac tcagcgatca ttcgcattga aaacacgatg gaatgtgaca
     1501 agtggttagc gaaaagcaaa gagaatttag ctgtagcgag attggtggat atggaggtta
     1561 taaacaatga agtttaaaat ctttgaagag gacactcgtt ataaattaga aaaagaatta
     1621 aacgattttg cgaaaaacaa tgagattcag catatatctt tagcaacttc taagagaggt
     1681 tatgcaaatt actatgcagc tgttgtgagc tatgtaagtc gagaagtgta actcggcaaa
     1741 taaaaaaagc cggatcgctc cgactgattc aataaatcca acacatttat tatatcacat
     1801 aaaggagcgg tttgacttga tgcaattgtt acgagaggta gatttcaaac agacaagatg
     1861 taatgcgaga gatgtgctga agaactttcg gcgtttggag cggatggcag gtcgctcttt
     1921 gatagatatt aagtcgccga ttattacgga tatgccgaag gcaccgaagc acggcaataa
     1981 ggcagaagac gcgatcattc aaatgatgga tatagaagcg gagagagacg cgattctagc
     2041 ggctttgatg gctcttagtc tgattagtcg tcagatactc tactacagct tctgtgtgcc
     2101 agatagcttc tcaaactaca ggattagccg tgaagtgggt tattcagaaa
//
LOCUS       DAFEHS010000145         2139 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFEHS010000145
VERSION     DAFEHS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.152"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            83..1071
                     /locus_tag="LP327_002738"
                     /pseudo
     CDS             83..1071
                     /locus_tag="LP327_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1306..>2139)
                     /locus_tag="LP327_002739"
     CDS             complement(1306..>2139)
                     /locus_tag="LP327_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL1726801.1"
                     /translation="VCAIGDEEMTIKERVSFPTTTPEETMPLVIDFFKQYQADLAGIG
                     IGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPISWTTDVNAAAYGEY
                     VFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPF
                     HGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFG
                     GGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLH
                     S"
BASE COUNT          696 a          437 c          391 g          615 t
ORIGIN      
        1 tccatgttcc ccactcctca agtgttttct ttagtatacg cctataggag gaaaaacgaa
       61 tacaatttaa ctcaatttat ataataggaa aaatttagcc gaatctgaag gaattagtag
      121 agttactcta tatcaatgga aaaacacggt tcttggaaag gattttccat tgcgtatgac
      181 tttaaaaaat caggaaaaac aaaaagagct acttttaaag gaagtagaag acttacaaaa
      241 acaagtacac caattacagc tcgaaaaagc attactcgaa ggagcggctg aactgctaaa
      301 aaaagaaaaa ggcgtcaatc ttctttgtct atcaaatcaa gaaaaaacga tattgattga
      361 tgccttacga aatcagttca ccttgaaaga gcttcttcaa cagctccagt tgcctaaaag
      421 cagctatttt tatcaaaaac aagcacttga gaaaccagat aagtattaca aagaacgtca
      481 attaattatc acgattttca atcataactt ctgtgcgtat ggttaccgta gaattcacca
      541 agctttaaaa aatatgggta aaaagctttc tgaaaaagtt gtacgtagat tgatgactga
      601 ggaaaatcta tttgttaaat tttctagacg aaaaaagtat tcttcgtatg ctggggaaat
      661 ttctcctgca caccccaatt tattaaatcg gaattttaaa gcaaagaagc caaatgaaaa
      721 atggttaaca gatattactg aatttagaat tcccgcaggg aaaatctact tatcaccact
      781 tgtcgattgt tacgatggtg ctattgttag ttggactatt ggaacaaaga caacttgctt
      841 cactctatgt ctaaaaaggg ttattcgcca gataattcag cgtgcgaagg tttctttgga
      901 cgattaaaaa acgagatgtt ttatcaacgt gactggaata atacaacaat caatcagttt
      961 ataaacaaat tggataatta tattcattgg tataataatc aacgaattaa gttatcttta
     1021 ggagggctaa gccctcttca atatcggaag aagcaaagat atatttgtta aaaaggtcca
     1081 aaaaaaatat ccgcacccct tttttttgta aaaaactatt gcacaataca agtagcctac
     1141 ggctatcgaa atttccataa ctttcgagca agaatttatc tcattcaagg cttgatcttc
     1201 caagtaaaac aaaaaccagt taagcactct gcctaactgg ttatgactaa attttcagtt
     1261 gtatctttct ctaccaacac taaatgtcga agagcctttt ttcgtttatg aatgtaagac
     1321 cgcttctcga gccaatgcca aacaaccgag cgttccagcg ttgtcttcta atgctggggt
     1381 cacgatatat ttgcttaact caggtgtttc gacataccca ttgaccaatt ttgcaaatgc
     1441 gtcgtggacg ttttgcagca tatggcgttg tttcatcaca ccaccgccaa aaatgatcac
     1501 atctggagaa aacatcaagg ttgtattgta ggcacattga gcaatatagg aggcttcgat
     1561 ttcccaataa ggatcatctt ctgcaacctc ttgtcctttt ttccctaagc gtttttcgat
     1621 ggcgggtcca gcggccatcc cttctaaaca gttgccatga aatggacagc tgccagcaaa
     1681 gtcatctttt ggatgaggga cgacgagcat atgccccatt tctggatgac taaacccttc
     1741 gataaagcgc ccttcttgta aggcaccgcc gccaacaccc gtaccgatcg tatagtaaac
     1801 gacactgctc aatcctttgc cactgccgaa gacatactca ccataggcag ccgcattgac
     1861 atctgttgtc caagaaatcg ggatcggaaa ttcttttttc atggtaccga tgaaatcaaa
     1921 attttgccat gccaatttcg gggtcgaggt aatgtaccca taagtggcgg aatcgcgatg
     1981 gatatcgata ggcccaaatg agccaatccc aatccctgct aaatctgctt ggtattgttt
     2041 gaagaagtca atgactaaag gcattgtttc ctcaggtgtc gttgtcggaa agctcactct
     2101 ttccttgatc gtcatttctt catcgccaat ggcacacac
//
LOCUS       DAFEHS010000146         2097 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFEHS010000146
VERSION     DAFEHS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.153"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(142..417)
                     /locus_tag="LP327_002740"
     CDS             complement(142..417)
                     /locus_tag="LP327_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBL1726802.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /gene="der"
                     /locus_tag="LP327_002741"
     CDS             complement(660..1970)
                     /gene="der"
                     /locus_tag="LP327_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBL1726803.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT          603 a          456 c          347 g          691 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaa
//
LOCUS       DAFEHS010000147         2000 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFEHS010000147
VERSION     DAFEHS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.154"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..376
                     /locus_tag="LP327_002742"
     CDS             <1..376
                     /locus_tag="LP327_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBL1726804.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="LP327_002743"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="LP327_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL1726805.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..>2000
                     /locus_tag="LP327_002744"
     CDS             1857..>2000
                     /locus_tag="LP327_002744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726806.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          552 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt
//
LOCUS       DAFEHS010000148         1929 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFEHS010000148
VERSION     DAFEHS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.155"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(121..1161)
                     /locus_tag="LP327_002745"
     CDS             complement(121..1161)
                     /locus_tag="LP327_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL1726807.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          673 a          287 c          231 g          738 t
ORIGIN      
        1 acttataaat tctattaaaa tagctattat tttgtaatat tcgtaatata attagaataa
       61 taaaaaattt ttaattttaa tgttactcca tgaagcaaca atcactttgt cagtaaagtt
      121 tcactctaac cagtctttcg taatagtgct tggggagctt tcataattca aacgtttttc
      181 acttgatact ttagcagcta ttggtttttc gataaaccaa ttttttacat agacaaaaat
      241 caggttctgg atattttcga ctttttcggt ttttaatttt tgataggctt ttaacagtgt
      301 cgtgtataat tcttgttctg cattgacata ataactatcg agttcttcaa aaactatcga
      361 aataccactt tctatttcag cttttttctt tgcattatga atagtctgga cggttttttg
      421 agcttcactg taacttgaag aaaatgtttt tatcaaattt aatactcgtt cagggacaaa
      481 cgtagcaatg ctgtcgtttg tcataattgt ctcaaaattg tctaacaaca attgatcttg
      541 taggcggtct ttttcagtgt ctatattgtg tctattagtg tctaatatat tattattaag
      601 ttctggtatg attttcatac cctctgattt atcaacgttt tggcacggta tgattttcat
      661 accctcttga gccccaaggg attcagcgtc tgatttttcg tcgagggtgg gtatgatttt
      721 cataccctct gatttatcaa cgttttccct attctctcta tcaaattttt cgagtaagta
      781 gatatcattt tctgagactt ttaattctgc tagatatagt ctattaggat tatttttccc
      841 taactgtttg ttaaatccca tctgcttttg taataataaa ccaaaggatt ccaactcttt
      901 tttgattgct atcgcttttt gtttttggca gtttaatact ctacatagtt catttgtcgt
      961 ataagtaaaa taaacattac cttcttcatc gatttgccgt ttgctgattg cgatctctaa
     1021 tcttgctttt aaaagcatat aggcaatctt agttgaatct ttcatttttt tgtattcgtc
     1081 agaaacaata aagacttttg gaaattgata aaataattcg ttgtaaactc tatccgcctt
     1141 aaaaaattta aaatcagtca ttgtagatcc tctactttca cgttgtttta tttatgtaaa
     1201 attacctact cttttcgaaa atagcaaaga taaataaaaa atgcaaacgc attaaaactg
     1261 ttttttaatt aaatactacc taaaatcaga aataaaatga attatgtact acctttaaag
     1321 tcattaagaa ctactttaaa taaatattat ttataattaa atactaccag aaaaacgtat
     1381 gaataaaatt tagttttaaa attaaagata attaagtact accagtgtca aaacaaaaaa
     1441 taaattaaga actaccttat gcatataaaa catactacca ttactagtaa tatatactac
     1501 cttatttcag tagtatatat tatattattc ttataagaca aggctttagg ctagaaaaca
     1561 atagattata tactactaaa aaatacaata agtactacca atgtcagata tatataataa
     1621 ttatgtacta ccattaaaat taacttattt aataagatac tactgtgtta atctgaaaaa
     1681 aattacttta aagtagttac atactacctg ttaatttaga tactaccatt aatacttaaa
     1741 ttagatacta cctatattta tattaagtac taccgttcta agaataagat aataaaaaaa
     1801 tactaccaaa ttgcaattaa atactactta aaatgtaaaa aataagaact acccaaatta
     1861 ggtagttctt aataagagaa tatcagataa atttgtacaa gtcataaaaa aattaatctt
     1921 aaattttta
//
LOCUS       DAFEHS010000149         1889 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFEHS010000149
VERSION     DAFEHS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.156"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(361..1479)
                     /locus_tag="LP327_002746"
     CDS             complement(361..1479)
                     /locus_tag="LP327_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726808.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
BASE COUNT          554 a          406 c          297 g          632 t
ORIGIN      
        1 acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa
       61 taatttttgt caatctcttc ttatttcttg aagctgacca cttctgtcac aacctcttcc
      121 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa
      181 gagctatttt tgcttgctgt tcttattact tgatgcagaa cagctttttc acaacctctc
      241 atacacggtg tttaatcagc tgaccttcgg tattagctca aaaaacggaa aaatatgtga
      301 aactattttt ccgtttgttt ctctaatact caggtcaaaa cactgatttc acaacctctt
      361 ttaataacga agaatcgctg tcagactctt ttcatctggc gcatcttttt gatccaaaat
      421 aaacactttc ccgccattaa tgatcacatc atctgctaat gcattcaaca cttgacgccg
      481 atcatattct gtttcaggat cttcaccgaa accatcagct aagtttgacg tagcgataaa
      541 taaatgtgac acttttcctt ctttcgcagc tggaacgata tcaactaact gatcgatgaa
      601 tttcttatct aagagtttat tgtattcttt tgattcttga gctgcgagtt cacttgacag
      661 cttcttcgcc ccttcttcga tatctctaaa ggatagctgc gcaggagaag cagaaatcgc
      721 aacgtctgca caataatagg gatttttagc aatttttttg aacatcgtct gattttctgg
      781 taatgcaaat aaataaatcg gccattgttc aggattttcg aaagaatcct tcaagtacgt
      841 atcaacagct tggtaatagt tgacccaatc gatctctact tcttcatctt ttggatttac
      901 gccatggaaa gttactccat ctcgggctcc gcctcctcca ttagaagaga agttcaaacc
      961 gccacctgtc agttcagatc ccaatgcagt aacgacatca accggtgccc cttctggtag
     1021 agatacttct tttacttgtt tgttgctgac ctcatacaat ttcatggagt cgcggttcaa
     1081 tcccaataaa taataagaat agttgaactg cccatttttg ataatagcca gtaaataagg
     1141 aagatcactt acatagtatt gatcgtctac cgggatactc aaacgatgga tataggtctg
     1201 gtcagcagtt ataatgaccg ctacactcgt agttgcacta cgccaaaacg aaggatctcc
     1261 taacaaattt tcaatttttt cttggaacgg ggaccagtca tgttcggaat attttttctc
     1321 aaaacgtttc tttgcttctt tcgcaaaatt tttgaatttg atctgatctt tttccacatc
     1381 ttgatgggcg acatgcgtat tcagcatgat cgtgacgaat ggtcctttag cgccttctga
     1441 agtcaattgt gtcaataatt ctttatcctt ttttgccata aacgtacccc tcctaaaaaa
     1501 atcattcctt tgtttctatc taaagtctac cacaacacta tttttccata caataaaacg
     1561 tatctaaaaa aataaaacgg tttctacggt tttaaattta gctatttttt taagttatct
     1621 tcataatttc ctcaattatt tagccccagt cttcaactat tcttcatgaa atgctgataa
     1681 actaagaaaa agaataaaag aggtggatct tatgcatttg attatgattt tcagtttatt
     1741 aagtattatc agtttatttg ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc
     1801 tattcctgta aaagtacgta aaaacgaccg ctaaacttcc ataacgcgcg ttcttatcct
     1861 aaagtttttt cagaaacatt gaaactctc
//
LOCUS       DAFEHS010000150         1866 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFEHS010000150
VERSION     DAFEHS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.157"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(64..573)
                     /locus_tag="LP327_002747"
     CDS             complement(64..573)
                     /locus_tag="LP327_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBL1726809.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(685..1683)
                     /locus_tag="LP327_002748"
     CDS             complement(685..1683)
                     /locus_tag="LP327_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL1726810.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT          568 a          355 c          333 g          610 t
ORIGIN      
        1 cttgaaacaa acaaaaaaga aagaactcgt ctaaatagac gttttctttc tttttttgtt
       61 gaattattga gtaaggcgac ttcttttttc tggttctttg tgtgctaatc gactggcagc
      121 agcagcggcg atcgctccga cgatatcatc taagaaggta tggacatgaa cgccatcgtg
      181 gctgtttagt tcttttaaaa taccaggttt caccttgtcg atataaccat aattcgtaaa
      241 gccgatcgta ccgtaaacat tcacgattga caaggctagg atctcatcga taccgtaaag
      301 gccttcgtca tcttcgataa tatgttgcaa cggactcatc aattttttct tttctgctaa
      361 tatatctaat tggattccag tgataatggc gttatgtact tcacgctttg ttagcacgct
      421 gttgacactt tcagtacaat ccgccaaagt caaatcagaa atatagtctt tttgtaaaaa
      481 catgacaagt tgtgcgatat cttctacagt tacgccacgt tccttcaata gttctcttgc
      541 cttttcttct aaagtctcag ttttgacgac catattattg ctcccccttt attttttctt
      601 tctctatttc atgttaacgg aacatgaaaa aaatgcaaga aactgctttc gggtttaact
      661 tgaaatgcaa agagatataa aaaatcaact aggacgaaga tttttatcgt ataagcttgt
      721 gctgtgacca ggctgagttc gttcctttgg attaatatag tgtaaagcgt tgttcactgc
      781 agtaggtgct tcaccgagcc cagtagcaat cagtttcact tttccttcat aggtacagat
      841 gtcaccagct gcataaacgc ctggaataga agtagacata tctgatttta cagtaatggc
      901 atttctggta ctatcaagtc cccaagaaga aagatgttct aaattagaag agaatccata
      961 attgacgatc agagaatcaa tcatcaaatc aatcgtctcg tcacttttca ctttttttaa
     1021 ccggatatct gtcagttccc cattattgcc tgataatcca tcgatgagat agggagtaag
     1081 caagttgaca gatgaagatt tcaagcgtga tacactatgt tcgtgcgcac gaaattccgg
     1141 gcgacgatga atcaagtaaa cttcgcttgc aataggctct aacatcaacg tccaatcgat
     1201 agcagaatca ccacctccag caatagcgac tttttttcca gcatacttca tcaaatcatt
     1261 cacgaaataa tccaatccgt ggttttcaaa tgattcagca ttatctaaat tcaattttct
     1321 tggctggaat gaaccatttc ctaaagctaa aatgactgct ttggaataat ggatgccttt
     1381 atttgtagta atctcgataa cttcgtcttc acgagtaagc gagagaactt cttcttttaa
     1441 atggaatgta tggttaaaag tagttaattg tttttctaat tgatcgatta attcacttgc
     1501 ttttattgct ggatacccag gaatatcgta aatatatttt tcgggataca aagttgctaa
     1561 ttgaccacca agttgaggta gactgtcaat gatttttgtt ttagcttggc gcattcctgc
     1621 gtaaaaagcg gcaaacatcc ctacaggacc tgcaccgatg attgtaatgt catagatttc
     1681 cataaaaatt cctccctttt cacactatag cagaatttaa ttgagaatgc ttctcaaaaa
     1741 tgtttgaatt cgaaatcgaa acggtaagtt atagaatgta tctagtgatg aaaaataaaa
     1801 gaaaacaaat ctagtatttt tacgagaggt taggtattat gttattatta ttatgaagta
     1861 tgcaaa
//
LOCUS       DAFEHS010000151         1817 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFEHS010000151
VERSION     DAFEHS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.158"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..1029)
                     /locus_tag="LP327_002749"
     CDS             complement(<1..1029)
                     /locus_tag="LP327_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL1726811.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFATSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1111..1800)
                     /locus_tag="LP327_002750"
     CDS             complement(1111..1800)
                     /locus_tag="LP327_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL1726812.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
BASE COUNT          604 a          412 c          320 g          481 t
ORIGIN      
        1 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
       61 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
      121 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
      181 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
      241 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
      301 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
      361 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
      421 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
      481 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
      541 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
      601 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
      661 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
      721 aaataacgta aacgaagtcg caaaaaaagc ggccaacaga gagacggcag ataacgctac
      781 cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa
      841 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
      901 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
      961 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtg ttttttcatt
     1021 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
     1081 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
     1141 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
     1201 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
     1261 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
     1321 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
     1381 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
     1441 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
     1501 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
     1561 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
     1621 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
     1681 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
     1741 taccagtatc agaatgattc ccaacgagat caacatcaat cggattattt tctttttcat
     1801 atttcctcct gaaaaaa
//
LOCUS       DAFEHS010000152         1755 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFEHS010000152
VERSION     DAFEHS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.159"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            192..446
                     /locus_tag="LP327_002751"
     CDS             192..446
                     /locus_tag="LP327_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL1726813.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="LP327_002752"
     CDS             447..764
                     /locus_tag="LP327_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL1726814.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..>1755
                     /locus_tag="LP327_002753"
     CDS             1323..>1755
                     /locus_tag="LP327_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726815.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT          625 a          249 c          327 g          554 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata
     1561 tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg
     1621 aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac
     1681 catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta
     1741 atcaaggaaa tatga
//
LOCUS       DAFEHS010000153         1745 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFEHS010000153
VERSION     DAFEHS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.160"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            378..1628
                     /locus_tag="LP327_002754"
     CDS             378..1628
                     /locus_tag="LP327_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL1726816.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          334 c          307 g          526 t
ORIGIN      
        1 taatagtttt tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa
       61 cggaatttgt ttcgatattg tgtgatttct cagagtacaa tggatgtatg gaaaggatcc
      121 aacgggaatt tgctatttcc tcctgtagcg tttgtctacc ctgtttagtc tgttacccga
      181 accactgtta tcactaccaa ctcattagct gtttcgataa tggactagac tttcaaatag
      241 tagtttggaa ggcagcttct agctctttta tcaccgtatt ctattaggcg aggttgtggc
      301 tatacagtga gtttctggga aatcccacag tatcgaagca ttttccaaaa tttatatcga
      361 aaggaggtcc ttcttccatg aaattatttg tcggtattga cgttagctct gaaaaactag
      421 atgtttgttt tttaacagac gacaatcaat tgtctatctt atctgaaatc tctgttgcca
      481 atgacatcga aggtgcttca ttcatacgag aaacaattct tgaattcaat aacagctacc
      541 actttgatca aattgtgatt ggcatggagt caacgtccat gtacagcttc catccttcca
      601 tgttttttca tgaagatgag aaactaaaga agctcaacac acttgtaacg attgaaaatc
      661 cttttcgggt gaaacaattt agtcgcatgt ttgatgagaa taaaactgat cgaaacgatg
      721 cattaagaat cgctgatttc ttgcgtattc aacgattcac aacttcccct atcaaagaag
      781 agaaatacat ggccttacag cgtttaacac gtactcgcta tcaacttatt aagcaattga
      841 ttcgtacaaa gcaacatttt cttgaaaatc taacgtacaa atgtaatacg cttgctcgag
      901 aaatgcgaga cgaatcgacg agtcttttta gtgcaaccct catttcactc atgacggaag
      961 atttcacatt ggatgaactt gctgagcttc ctttagaagc tttctgtgat ttacttcaag
     1021 aaaaaggaaa aggccgtttc aagcaaccag aaaaaatcgc taaagcaatt caacgtgcaa
     1081 ttaccatgag ttaccgccta ggtgctcttg cacaagaatc aattaatgtt gttttaagtg
     1141 ttctcgtacg agaaatacga gcacttgaaa aaaatatcaa agaattagat aaagccatcg
     1201 aacaagtaat tgttgtcatt ccagaatatc aatgcttaac cagtattcca ggcgttggta
     1261 aagtctatgc tgccggttta atcgctgaaa ttggtcaaat cgaaagattt gaagatcaga
     1321 caaaattggc aaaatacgct ggattaagtt ggaaagtgaa ccaatctgga aactatcagt
     1381 cgcaaaatac acctctgaca aaacaaggaa atcgatattt tagatactac ttagttgaag
     1441 ctgccaactc tgtaaaaaat tatcttcctg aatacaaagc tttttatcaa agtaaatata
     1501 aggaggttcc aaagcatcaa cacaaacgtg cactcgtcct aaccgcaaga aaatttacgc
     1561 gtctggtgga tacgctacta cgtaaacacc aactctatac gccaccaagg agtgtgatag
     1621 agaaataaca atccgttatt gacgcaaatc cttgaatcaa cccgaaaaaa atttgatttt
     1681 gatcgggtct agtttcgtgc gcttcaaaac atattactca ataaagaaaa tcttaaattt
     1741 catct
//
LOCUS       DAFEHS010000154         1649 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFEHS010000154
VERSION     DAFEHS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.161"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            237..1454
                     /locus_tag="LP327_002755"
     CDS             237..1454
                     /locus_tag="LP327_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL1726817.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
BASE COUNT          538 a          353 c          313 g          445 t
ORIGIN      
        1 ggaacatcat tgacgaatga tgttctcctc tattttctat ttttagatta tagtgaagag
       61 tgaaagaaag ttcgaacaaa gccttgagga ctacgagtcc ctatcaaaaa ccgaacttct
      121 ttactgtcat tttagaatca ggtttacgta tgttggggcc atgagcccgt gtataggaaa
      181 ttggaatcga aatgaagcag taaagttctt tcaaaactta aaaaggagga aaacaaatgc
      241 cttacattct tgcattagat gtttcaatgg gacacagcta cagtgtccta tacaaagacg
      301 acacctgctt atttgaagac gagattgaac acactcaaag aggatttcat gcattactcg
      361 aagagatcca gcacttgcct gagcgtccat tgattgtttt tgaagcaacg gggatttatt
      421 ccagaccagt cgaaaagttc tttcaagatc atcacttttc ttactgtttg ttaaacccgt
      481 tagaggcgaa aaaacagatg gaagaaagca ccttacgcag ttggaaaaca gataaatcgg
      541 atgcccaccg gctcgcacag actcatctta aaaatcaacg tcagccaaaa gaagtacaaa
      601 gcaattttta cttagagatg cgcgatttag cccgttttta tcaagaaatt gagaagaaaa
      661 tcactcgtct gcgcatggat ttacacaact gccttcaatt gacttttcca gagcttgaac
      721 agttcttttc aaacaggctg accccttacg cattgacatt gatccgtctt tttccccatc
      781 cagattttgt attggcatct actcggacaa aaataaaaaa taaactgatc aacgaaacac
      841 ggaaaaaaat ctccgcgaat cgtgccgagc aaaaggcaga tcaaatcatc cactatgcac
      901 agtgtgccta tccagctgtt gaaaaagaca gtatccactg tcaaaaaacg atctattacg
      961 ccgaacttct ccaagattta ctcgaacaaa aaaaagcact ggcgacacaa atgatcaaaa
     1021 aagccgaagg ctctccttgc tttttgttgt accaaacgtt ccctgggatt ggcgcactca
     1081 cagccgcttt attgctcggt gaattaggag atatcacacg atttaaaacc cataaacaat
     1141 tgaatgcctt tgtcggaatc gacatccgac ggtatcattc aggtaagtat actggccaag
     1201 accgcatcaa taagcgtggt aatcccaaag cacggaaaat tattttcttc accattcgaa
     1261 atatgattcg ccagcaacgc gcagctccta atcatattgt cgattattac tacaaattaa
     1321 aaaagcaacc tatccctaag aaagataagg ttgccacagt tgcttgtatg aacaagcttc
     1381 tgaaatgtat gcacgctatg gttagggcac atacagagta cgactatgcg tacgcggtct
     1441 cagtggacca ttaactaaac cacttacagt ataccacttc tttgcccaga tcgaaaattt
     1501 tttacgaacg gggctttatt tggtgtgctt tgattttgtc gctttcgttt acgagctatt
     1561 tttcttgcac cttttacttg actaatcgta ggaaaagagg ctgggacaga agcgtttaac
     1621 tccaagaaat aagaagaaac tcaagaaaa
//
LOCUS       DAFEHS010000155         1632 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFEHS010000155
VERSION     DAFEHS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.162"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="LP327_002756"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="LP327_002756"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="LP327_002757"
     tRNA            203..276
                     /locus_tag="LP327_002757"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="LP327_002758"
     tRNA            316..405
                     /locus_tag="LP327_002758"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="LP327_002759"
     tRNA            420..491
                     /locus_tag="LP327_002759"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="LP327_002760"
     tRNA            507..579
                     /locus_tag="LP327_002760"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="LP327_002761"
     tRNA            589..661
                     /locus_tag="LP327_002761"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="LP327_002762"
     tRNA            697..772
                     /locus_tag="LP327_002762"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="LP327_002763"
     tRNA            774..854
                     /locus_tag="LP327_002763"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="LP327_002764"
     tRNA            861..934
                     /locus_tag="LP327_002764"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="LP327_002765"
     tRNA            937..1009
                     /locus_tag="LP327_002765"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="LP327_002766"
     tRNA            1021..1092
                     /locus_tag="LP327_002766"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="LP327_002767"
     tRNA            1138..1208
                     /locus_tag="LP327_002767"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
     gene            1229..1313
                     /locus_tag="LP327_002768"
     tRNA            1229..1313
                     /locus_tag="LP327_002768"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:caa)
     gene            1375..1447
                     /locus_tag="LP327_002769"
     tRNA            1375..1447
                     /locus_tag="LP327_002769"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1408..1410,aa:His,seq:gtg)
     gene            1457..1528
                     /locus_tag="LP327_002770"
     tRNA            1457..1528
                     /locus_tag="LP327_002770"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Gln,seq:ttg)
     gene            1546..1630
                     /locus_tag="LP327_002771"
     tRNA            1546..1630
                     /locus_tag="LP327_002771"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1580..1582,aa:Leu,seq:caa)
BASE COUNT          380 a          371 c          431 g          450 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta
     1321 agatgtcttc atcttagttt tttattgaga aattaaataa catcttttat gatggcgaat
     1381 gtggcgaagt ggttaacgca ccggattgtg gctccggcat tcgtgggttc gattcccatc
     1441 attcgccctt tattcttggg gtatagccaa gcggtaaggc aagggacttt gactccctca
     1501 tgcgttggtt cgaatccagc taccccagtt attattttta aatttgccgg cgtggcggaa
     1561 ttggcagacg cgctggactc aaaatccagt gtccgttgag gacgtgccgg ttcgaccccg
     1621 gccgccggta ta
//
LOCUS       DAFEHS010000156         1586 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFEHS010000156
VERSION     DAFEHS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.163"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            294..1481
                     /locus_tag="LP327_002772"
     CDS             294..1481
                     /locus_tag="LP327_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL1726818.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          468 a          295 c          316 g          507 t
ORIGIN      
        1 ggttttaacc ttccggtttt gattggataa ccctatgaac catcgttatt ttaatttggt
       61 attaactcat ttcttttgag ataatatatt tattggattg agaggctaag ccgttccccc
      121 cttgggtcat tgaacccgca ctcgagagac tggctcctct ctctgataag tgtattgcgt
      181 atgagttaga tgttgaaagt ccttacttta ctccgtatct cgtacaaggt agtgacgctt
      241 ttcagttgag tggatttcaa tccaaaattc tttttttgaa aggtcagata tacatgtact
      301 ttttaggcat tgatatcggt aaacgcactc acgttgcttc tatcatgaac gaggaaggaa
      361 aggtattact aaaaggtttt tcatttccta acaccactga aggagcagag tcattaattg
      421 agcgaatggt cgactattcc ggagcaccca gtgactttgt tatagggatg gaagccacgg
      481 ggcactattg gctgtccatc ttttcatacc ttcacgaatc agattatttg attcacgttg
      541 tgaatcccct tcaaacagac ggatggcgta aaggtacaga aatacggaaa cgaaaaaacg
      601 atattattga ttctgttttg attgctgact taatgcgcta tggctcattt gtcgaaacgg
      661 tcttatccga tgaaaatgtt ttttctttga aacaactttc tcgttaccga acttatttag
      721 taggtacagc gagcgacttc aaaagaaaaa ttatagccgt attggatcaa gtctttccag
      781 aatacgcgac gatttttacc aaacagggcg ttttcggaaa agcttctaaa gagttattgc
      841 tggcgtttag tgccccgatc gacttagaaa acatttcctc agaaactttg gcccaatact
      901 tagctgagtt aagcaggaac cgaatcaagg cagaaaaagc ggaccaattg aagcaagcag
      961 cttctaactc ctttggagta aaatttgctc aagaagcgtt caccttccaa ctacgttcaa
     1021 tgattgaaca attaaaattc attgaaagcc aaataaagga aacggaaaga gaaatcaagc
     1081 acataatgga cactttaaat tctgttattc tcacaattcc tggaattggt cctataaacg
     1141 gtgcgactat tcttggtgag atcggagata ttcaaaaatt ttctaacccg aaaaaactcg
     1201 ttgcttacgc tgggatagac gcttccgtga cacaatcagg gcaatatgag gcaacgcata
     1261 atgttatgag taaacgaggg tctccatatt tgcgaaaagc cttgttttcg gcggctttag
     1321 tagcttctca atgtgatcct gtttttagtg cgttttacca gaaaaaagtt tccgaaggaa
     1381 aacatcatct aactgcctta ggtgcggttt ctcgtaagct ttgttacgtg attcatgcta
     1441 ttttgactaa aaacgagcct tatataattg tttctgatta gctattgaac attcaaaaaa
     1501 actaatggta agaagcttat ttgttgtacc cttttttcgg aattatctgt tataaaatat
     1561 tttttgattt ggctaatttt tacttg
//
LOCUS       DAFEHS010000157         1560 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFEHS010000157
VERSION     DAFEHS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.164"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            393..668
                     /locus_tag="LP327_002773"
     CDS             393..668
                     /locus_tag="LP327_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726819.1"
                     /translation="MYKPYMISYDLNNPGKKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            complement(665..817)
                     /locus_tag="LP327_002774"
     CDS             complement(665..817)
                     /locus_tag="LP327_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726820.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(1075..1461)
                     /locus_tag="LP327_002775"
     CDS             complement(1075..1461)
                     /locus_tag="LP327_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726821.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            complement(1474..>1560)
                     /locus_tag="LP327_002776"
     CDS             complement(1474..>1560)
                     /locus_tag="LP327_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL1726822.1"
                     /translation="ERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT          511 a          308 c          214 g          527 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaaaacaat agacagcaac aaaataacat tgctttgata atttggtata aaccactata
      181 aatttctttt cttgcaatta tttttaatat atgctagatt atcaaccgat atagtcactg
      241 cctgtactag cggaaactag tgcaggtttt ttgttctatt tactcaaaag ttattacgat
      301 aaattaatat tgtgaaaata aatactaagc gtataatttt atttatcagc gagtggtccg
      361 ctgaaatata aaacaaggat gtgcaagaca acatgtataa accctacatg attagttatg
      421 atcttaataa cccagggaaa aagtatgata aagtatttga aataataaaa gaatttgggg
      481 catatataaa gctacaaaaa tctttttggt tagttaaaac taatttgaat ccaaatcaaa
      541 tgtgtgaaaa attaaataca gtacttgata ataacgattc cttattcatc tgtgaactgc
      601 aaaaaaatta tcaaggtaga gctacagagg aaaattggaa attcattaac gaacatattt
      661 tctcttagta aggattagat attttttctc tgtttaaaca attgcaaata ccgtcaatat
      721 ctgaattaga aacacttacc ttttgctcct tgctactacc aatagcttgg agcaattctt
      781 gtatttcttc tggagtgcct tctactgata atttcatttt tcttccctcc aatacataaa
      841 ttaatagaca gcaacggatg atagataata agaataattt agaaggagtt gaaattcaca
      901 tccttattct taatatttcc gctgctgtct atcgaagctt aattaaacga tgagggagat
      961 ttcctccctt acattttatt ttgtcgatcc tgtttcctaa tctttcgaca ctaccataat
     1021 atcactgata aatggctaaa aaccgccatc attccgccaa aaaaccgcca ttttttatgc
     1081 gtcagtcact atacaaagtt tgtctttgac ctttaaagca caaacactat aatagtagcc
     1141 gccattccca tcattcgctg tgcattctgc tttagcgatc tcattacgat tatgataaac
     1201 aacgacttca gcataagagg tatgtccgtc accattatag agacggttac ctttatcaaa
     1261 aatctttata tctgtaatca ctgcatcaag ctttacattt ttgaattcac cttcggccca
     1321 tgcgcagcaa tctgattcgc tacatacaac ttccattttg gttccatctt ctaaaattaa
     1381 ttcactctct gaccattcta caattctttt gaaaacaaga tcttttttca actctttcaa
     1441 cgatacataa tctttccaca ttatatggtc cccctattta taagcaatta ttcttccgtg
     1501 tttatacgct tctgcaaact ctattagagc ttccgacttc atcctttgta tgcttctttc
//
LOCUS       DAFEHS010000158         1525 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFEHS010000158
VERSION     DAFEHS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.165"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(191..763)
                     /locus_tag="LP327_002777"
     CDS             complement(191..763)
                     /locus_tag="LP327_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL1726823.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(779..1384)
                     /locus_tag="LP327_002778"
     CDS             complement(779..1384)
                     /locus_tag="LP327_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBL1726824.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          427 a          329 c          218 g          551 t
ORIGIN      
        1 atatgatggt atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt
       61 acagttacca tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca
      121 caacatgcta atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga
      181 gacttctttt ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga
      241 aaccggttat agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt
      301 gcctcttctt cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct
      361 ttcttgcaaa catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg
      421 ctacactcaa caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta
      481 gattcaaaac cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt
      541 ctctcgtgtt acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca
      601 gaccagccaa cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg
      661 aaaaaatatt cttcttctct acgccgttct caagcaactg ttgaatttgt tcttttgtac
      721 tttgagaagc ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt
      781 acaaatcatc tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt
      841 tcatatataa ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta
      901 tttccaaatc gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta
      961 agttccaatc tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag
     1021 tacttcggcc atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga
     1081 taatctcttc aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat
     1141 acataactgg tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg
     1201 caaaatcgta aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac
     1261 caacttcgat ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga
     1321 gtttttcttc aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa
     1381 tcatgcgaaa tctccttaac tttatgacat aagtttatga aacactctca accccattat
     1441 atcgcgattt tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca
     1501 agtgtctcta ttttgcacag tctag
//
LOCUS       DAFEHS010000159         1509 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFEHS010000159
VERSION     DAFEHS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.166"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..269)
                     /locus_tag="LP327_002779"
     CDS             complement(<1..269)
                     /locus_tag="LP327_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL1726825.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNEN"
     gene            complement(829..1032)
                     /locus_tag="LP327_002780"
     CDS             complement(829..1032)
                     /locus_tag="LP327_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726826.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(1232..>1509)
                     /locus_tag="LP327_002781"
     CDS             complement(1232..>1509)
                     /locus_tag="LP327_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL1726827.1"
                     /translation="VQESLDSFASPHFLPIDIKPIDKIVIDGLIAEPSNWSIIARHTK
                     YKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLDVTEK"
BASE COUNT          431 a          342 c          227 g          509 t
ORIGIN      
        1 tgattttcgt tcaaacgcat taattgctcg aaatcgacaa aaacaactcc atttttcgtc
       61 caataagcta tggtatcatt cagttttccg acaattgtaa ttcgatcaat cgaccaattt
      121 aattttaggg gggtggtcgt accttgcaca cttaaccgcc tgttagaaat acggttaagt
      181 ttttcgagct gatttatcgt tgttcttttt tctgcccctc gaccttcaat ttttgattga
      241 atttcactca actgattttc atttgccatt ttcgtcctcc aaacgaaaaa agagactttt
      301 ggaaagaatt tccagagttc acgtaaccct aaaaattcat atccaaaaat ctctagcttg
      361 aagaaaccta aaaatattcg tataatgaac ctgagttacg gagctaggtg aagtagtttc
      421 cgtaaatagt cgagagtgtt agcgcactgc tcggctttta ttttgctctt ttttctcgtt
      481 ttctttgatt atagcactcc tcctaaaaaa ataaaaagac ctcagggaat ttcattcccc
      541 gaaccccttg agcctcctcc tgccgaaatg gaaagggcgc acccgctgtt tatttcattc
      601 agctatcggt tttcacaatc taacacgatc gttccgctac cagtcgcctt acaggctcca
      661 ttttcgctcc tctaacgtgt tttaacgatt gtgccgataa ctaaacgaaa taaacgctaa
      721 aaccgctcag aacgcaaaat atgccatttt agagctattg tagaaaatgt agtttgaaat
      781 gcaattttct tctttcagct ttttatttcc cacttgtaaa tctgctaatt attttctacc
      841 agacaaacca ttgatttttt ggctatcagt taactcacga ttattatgag aaatgcttgt
      901 attctttgtt ccctttttga acgaaatact aaaccctaag gtgaacccct ccttcacgct
      961 gtcgctaata aaaagagtgt aaacctttct gttactcttt gacaaagagt aacattttcc
     1021 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     1081 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     1141 gcttcatgca aaatattcgc ttatcgtgaa tttacgtcac taaacattga cgcaaatcca
     1201 ctctcactca tctgactttt cttattcggt atcacttctc tgtcacatcc aaccccaaaa
     1261 gccacgtatc caattctttt tttaattcgt ctgcagattc cttaaaagtc tctcgtaaat
     1321 gattcgttaa ctcatcattt tgactttctt gcttcagcaa attccgatat ttatattttg
     1381 tatgtctagc aattatcgac caattagaag gctcagctat caaaccatca ataactattt
     1441 tatcaatagg ttttatatca atcggcaaaa aatgcgggct tgcaaaacta tctaagcttt
     1501 cctgaacca
//
LOCUS       DAFEHS010000160         1480 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFEHS010000160
VERSION     DAFEHS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.167"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            68..151
                     /locus_tag="LP327_002782"
     CDS             68..151
                     /locus_tag="LP327_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL1726828.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="LP327_002783"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="LP327_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL1726829.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="LP327_002784"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="LP327_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          563 a          235 c          251 g          431 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa
//
LOCUS       DAFEHS010000161         1456 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFEHS010000161
VERSION     DAFEHS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.168"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            108..1040
                     /locus_tag="LP327_002785"
     CDS             108..1040
                     /locus_tag="LP327_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL1726830.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="LP327_002786"
     CDS             1112..>1456
                     /locus_tag="LP327_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726831.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DAFEHS010000162         1437 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFEHS010000162
VERSION     DAFEHS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.169"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..340)
                     /locus_tag="LP327_002787"
     CDS             complement(<1..340)
                     /locus_tag="LP327_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL1726832.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
     gene            564..935
                     /locus_tag="LP327_002788"
     CDS             564..935
                     /locus_tag="LP327_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726833.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1191..1433)
                     /locus_tag="LP327_002789"
     CDS             complement(1191..1433)
                     /locus_tag="LP327_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL1726834.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT          455 a          272 c          235 g          475 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaaaaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gagaaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagacat catccataaa atggactttg
      421 tctacagtct gacattattt tgataataat gattatttat ttaaaaatac attagataac
      481 atttgttacg acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa
      541 ctcacacaat taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg
      601 tgatctatat tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg
      661 atacgattgc tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta
      721 cgagctatta ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct
      781 ttttcaatct ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc
      841 atttgtggat catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta
      901 tctcaagaaa atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga
      961 cacatttaag gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc
     1021 tgtgacaaaa gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat
     1081 cttcgataat atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct
     1141 tattacttga tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt
     1201 cgagcaaaga atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt
     1261 gccattcctg caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca
     1321 actaaacctg ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct
     1381 cctgcaattg ctccgataat tcctccgacg ataagtgacc aaataaatcc catgttt
//
LOCUS       DAFEHS010000163         1424 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFEHS010000163
VERSION     DAFEHS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.170"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(150..1337)
                     /locus_tag="LP327_002790"
     CDS             complement(150..1337)
                     /locus_tag="LP327_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBL1726835.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAFEHS010000164         1406 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFEHS010000164
VERSION     DAFEHS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.171"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            97..1398
                     /locus_tag="LP327_002791"
     CDS             97..1398
                     /locus_tag="LP327_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL1726836.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          462 a          284 c          258 g          402 t
ORIGIN      
        1 atcattctat cggtcccttt tgagcatgaa aaaacaaaac atacagaagc tccgttagaa
       61 ttgaatcacc acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcatatcaaa
      121 ctactacttg gaatcaaaga taaaaacatt ttcatcacaa atgtggaatc gaagagcatt
      181 aagaatatca agagtcttgt tgtttctgcg acgttaagta aagaaattcg tcgctgtcct
      241 ttgtgtaaac agatgaatca tgaaggcatg atcgttaaga acggaaagaa aaaatcgctc
      301 attcaactta ataaatgtgc gaaccaactg acttatcttg ctttagcgaa acaacgctac
      361 cactgtcgtg gctgccacac ctattttacg gcgaatacct acattgtcga tagaaactgc
      421 tttatcgcta aacaagtgcg ctataaaatt ttagaagagc taactgaaaa acaagcaatg
      481 acgacaattg ctaagcattg cggcgtttct tggtcgaccg tttctcggac actcgcttct
      541 ctgatcccca tgacaaaagt aaaaagaaat tggttaccgc gttgtttatt aatggatgaa
      601 tttcgttcac tgaaaaatca agtcggtccc tactcctttt cttgtatgga tggcgatact
      661 ggaaaattac tggatattct tccttcacgg aagaaaaagg ctctcgtttc ttattttatg
      721 caatttgaac gacgcgcacg attgaaggta aaggtcatcg tgacggacat gaatgtttct
      781 tatgcctctc tgataaagga atgttttcca aaggcaaaac tcgttgtgga tcgttttcac
      841 attgtcaaac atctcattcg taaatttgag gatattcgcg tacgaatcat gaaaagcttt
      901 gatcgaaacg atccaacaca agccaaatat tatcgacaat taaaggcgtt gtctcggctt
      961 ctgatcaaac gacaggacaa gcttgtttat gacaaatgga ccaaatggcg caattttggt
     1021 tgggcgtatc tcaccgaaag tgaagtggtg gatcgtctgc tttcgatctc tgacgaatta
     1081 cgaatcgcct atttttacta tcaagaaatt cttcaagcgt ttcacgacaa agaggcggac
     1141 actttcttta aacttgtaag aacgatgccc aattccgttc caaaagaact gcattctata
     1201 aaaaaagctt ttataaacta tgaatccggc attcgtttag ccctcgaatt gccttattca
     1261 aatgccaaaa tagaaaatct gcatacccac atcaaagcat tgaaacgagt cgcttatggc
     1321 tttcgaagct ttcggaaaat gaagaccaga attttcttac taaataatct gattacctat
     1381 gaatcaaaaa acatctaaag tagaaa
//
LOCUS       DAFEHS010000165         1393 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFEHS010000165
VERSION     DAFEHS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.172"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            81..1373
                     /locus_tag="LP327_002792"
     CDS             81..1373
                     /locus_tag="LP327_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL1726837.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          457 a          282 c          255 g          399 t
ORIGIN      
        1 attttccaaa acatatagaa aagacacctt gaaatccttt agaattaatt tggcgaacca
       61 acccaaaagg aggaatcaag atgtcttaca ctcaccttat caaagaaaca ttagatattc
      121 tagacttatc agttactttt aacgaaaatt gtctcacaaa agaaaaatac aaagggcaaa
      181 tctgtcatat ttatcgtgga aacttgattt atacagccca agaatgcatc cactgtaaac
      241 accagattgc ttcagatatt gtgcgttggg gtacaacaac cgtccggttg ttaatgaacg
      301 atgtttctga ataccgtacc tacctcgaat tgaaaaagca acgcttcaaa tgcaaagctt
      361 gtcagcggac ctttgtcgca gatacttctg ttgcgaaaaa acattgtttc atcagtgaga
      421 aagtccgctg gtctgtcgtg actcgtctga aaaaaaatac ctcaatgaca gagatcgccg
      481 ctcagaaaaa tctatcggtc tcttctgttt accgtattat gaagcgattt tatcgtccgt
      541 tgaatccttt tcggacaccg ttacctaagg tactttgttt tgatgaattc aaatcagttc
      601 ggggtgtttc aggtgctatg agtttcatca tgatggatgg acaaacacag cgcttattgg
      661 acattgtcga gaaccgacaa ctgccttttt taaaacgtta ttttagtcat ttttcacgag
      721 aaattcgtga agcggtcgaa tggatcgtga ttgatatgta cgctccttat gtatcgctcg
      781 ttaaaaagct gtttccaaaa gcacagctga ttattgaccg gtttcacatc gtccaacata
      841 ttggtcgtac ctttcgcaat catcgaatca aagaaacgaa ccaactatta aaaagcaaag
      901 aacaaaaaca ctatcaatta ggtaagcaat taaaaagata ctggaaactt cttcaaaaag
      961 atgaacgcaa gcttgattat actcgacgcc tttggcgacc tggcttcaaa gctcatttaa
     1021 cagagaccga tattgtggat cgcttactga aagggagccc tgctttgcgt gtaggctatc
     1081 agttatatca agactttctt tacgctgtaa aggaacgaga ctacgtatcg tttgaagaac
     1141 tacttaccaa taatatcatg cttcccgaag gctatcagac gaccttgaga acgttccaga
     1201 aatttctgcc acaaatcaaa aatgctttac aacaaagtta ttcaaatggc ccacttgaat
     1261 gtctgaacaa ccatatcaaa gtgttaaaac gaaatgcata tggctttcgt agcttttata
     1321 atttcaaatt gcgcatcatg attcgtcatg gcaacgcatt gattttcaac taaaaaagat
     1381 caagaggaac tga
//
LOCUS       DAFEHS010000166         1378 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFEHS010000166
VERSION     DAFEHS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.173"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            51..1358
                     /locus_tag="LP327_002793"
     CDS             51..1358
                     /locus_tag="LP327_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL1726838.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT          476 a          268 c          295 g          339 t
ORIGIN      
        1 gataaaattt taatttgtag cgaaacaaat taattacaag gagatcggat atgtccataa
       61 aaaaaacacg tctagtccta ttattggcta gtctcattct ttgccttgct tcttttctac
      121 cagctgttgc tgtgcaagct gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg
      181 tagatgcgca aagcggtaag attctttatg atcaagatgg agaaaaaacc atggggatcg
      241 cttcaatcac aaaaatcatc ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac
      301 tgtcatgggg tgataaagtt tcgatttcag attatgcaga aaatttgagt atcaccccag
      361 atttatcgaa tgttcctttg cataaagaaa acacctacac tgtcaaagaa ttattcgact
      421 ctgcaatcat ccagtcagcc aatgcttcaa tggtggcctt ggcagaaaaa atctctggca
      481 gcgaagctaa attcactgaa cgaatgaaag aacaattgaa agattgggga atcaaagatg
      541 caacaatcgt taatgcatca ggattgaaca actcttatct tggcgaaaac cgaccagaag
      601 gcactggaga aaacgacgaa aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc
      661 atttgatctt ggatttccca gaaatactgg atgtatcaag tactacgacc caaatgtttg
      721 gcgaaaatac gcaatcacca gtggaaatgg tcaactggaa ctggatgctc ccaggattcg
      781 ttaattacaa ggaaggcgtg gacggattaa aaactggtac gactgagctt gcaggtgctt
      841 gtttcgttgg tacgatcact aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg
      901 acggccatgc ggaaaacccg agtgcccgct tcaatgaaac agaccgtttg atggactact
      961 gctatgacaa ttggtcagaa aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa
     1021 aaaccattga tgtaaaagac ggaaaagaat caacagttcc tgttgtgctt aaaagccctg
     1081 taaaagtatg ggtacgaaac gatatggata ctggcaaatt aacgatcacg ccaacacttg
     1141 acaagaagca agtcactgac aatgagatcc aagcacctat ggataaaggt acaaaaatcg
     1201 gtaccgctac gatcaccttg gcagacgata aattaggcta tttagaagac aacaatgcac
     1261 catcaacaga gatcattacg gataaaacag tggaaaaagc gaatatattc gtgattggct
     1321 ggcgacgcgt cgcagaattt ttcggcaact tgttttaatt gaacaataag aaaaacgg
//
LOCUS       DAFEHS010000167         1368 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFEHS010000167
VERSION     DAFEHS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.174"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            82..>1368
                     /locus_tag="LP327_002794"
     CDS             82..>1368
                     /locus_tag="LP327_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBL1726839.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAFEHS010000168         1352 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFEHS010000168
VERSION     DAFEHS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.175"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            306..848
                     /locus_tag="LP327_002795"
     CDS             306..848
                     /locus_tag="LP327_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726840.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1004..>1352
                     /locus_tag="LP327_002796"
     CDS             1004..>1352
                     /locus_tag="LP327_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBL1726841.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          524 a          163 c          258 g          407 t
ORIGIN      
        1 aggcttacca gcgagataga gatcgacgat ttgttgtttg aattcttttg aataggttct
       61 tcgttgacgt cttggcatga aaaatcctcc agtgtgtttt ttattattct acacacctta
      121 ttttttctgt ctagtctagt gtagccgatt caagttcttc gtgttctctt agacataaaa
      181 attcctcctg gtatgttttc ttctagtcta cacaccttaa tttttctgtc tagttaattg
      241 tagcctatcc agtttggaaa aataaaaaat tatatttcat caacgtttca taaaaggtgt
      301 gtagaatgtt attgaaagta gctataaaat gctattgtgt aaaaaataaa ggaggaataa
      361 tttatgaaaa aatagtacca agaggagcat gcctattact tgcattatgt ttgagcttaa
      421 gcgcagttgg gtgtcaaaaa agtaatgaaa acaataaaat aaaagaagga caatccataa
      481 gttctgaaga ggaaggcatg tcaattaatg caaatggcaa aaatgaaact tttaagccat
      541 cagattatac attggaggca aagaaagaat atgtttatga atatttaggg cttaaattta
      601 aactctcaga caaatttaga aattatatag ctgataaaaa gatagcgatg ttagatgatc
      661 aaagtcctat agataaagaa ctaaagtatg ctattttgac atttgagaaa atgacagaag
      721 aacaaaagaa tgcagttata gaaaagatgg gcgatggata taaaaattgg caaaatgagc
      781 ttgaaagaat tgggactatt ggtatatttg aaaaaaatac atcagaagaa aaaaatctaa
      841 aattataaaa tgtgatactc atactaaaat aggagtttca agtgatggaa aatacgattg
      901 ctatttaagc acaaatagcg gagctgaaag taatcttctt gatgaattaa aaagaactga
      961 gattcaaatt atagataaaa aagaacgtcc taaaaatggt tttgtgctat cagaaaaaac
     1021 tgatttagaa aatacagaag catttaacaa agaatcggtt aaagatttac gtaaactacc
     1081 aacaaaggac ataaatggca aggattttac aagtaaagat tttgagaagt atgatcttac
     1141 tatggtaaat gtatttgcaa cttggtgtac agcttgtgta aaggaaattc ctgatttggt
     1201 tgaggttcag aatgaaatga aaagcaaggg tgtaaatatc gttggagttg ttacagatcc
     1261 tgttgatgat aacggtgaaa acaaggaagc aattgaaaag tcaaagttaa tacatgaaaa
     1321 gacaaaggct tcatatcctt tcttaatgcc ag
//
LOCUS       DAFEHS010000169         1337 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFEHS010000169
VERSION     DAFEHS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.176"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..1337
                     /locus_tag="LP327_002797"
     rRNA            <1..1337
                     /locus_tag="LP327_002797"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DAFEHS010000170         1333 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFEHS010000170
VERSION     DAFEHS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.177"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..500)
                     /locus_tag="LP327_002798"
     CDS             complement(<1..500)
                     /locus_tag="LP327_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL1726842.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAK"
     gene            complement(774..1034)
                     /locus_tag="LP327_002799"
     CDS             complement(774..1034)
                     /locus_tag="LP327_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726843.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          464 a          240 c          198 g          431 t
ORIGIN      
        1 cactttgcta gggggcgggc ttgccattct tccaattcag gaaggacagc gtcagtgata
       61 tctgaaatca tttcatggga aatatcaaag ccataaatag cttcgacggt tgcggcaata
      121 tctcgttgac tcattcctcg tgcatacatg gaaagaacct tcccttcgat gtcggagaca
      181 tctcgttttc tcttaggaat taactctggt tcaaaggaag cttcccggtc tctaggaaca
      241 tcaatagcta cttcaccaaa actggtttta agcgttttag ttccatagcc atttcgacgg
      301 ttatcgtgtt ccttaggctc tttagaatgg gcatcataac ctaaatgatt attcaattct
      361 ccttgaagca ttttttcaaa aaggggccca aacacatctt tcaaagcatc ttgcatgtca
      421 tcgacagatt caggttgata ggcattcaga atggattcag ctaacttttc ggcatcagga
      481 tttcttttct ttctagccat cgtgtaatca cctcttgatc ttattgtaga aaaagaaaaa
      541 ccgcgaagca accacctgcc taggattaat ggttacttac acggtttata ttacactctc
      601 atcattaaaa aagtatcttt cgatattact aaacaatgaa attatctatt tgaaatttaa
      661 atctgataat atattgagtg ttccaatgag ggctttgatc ctattatcca tatctttttc
      721 agttttaata ttgatacaca ttataagaaa aagaaacttt tggttaattt atttcatccc
      781 agtgcaaaat ataaaaaagt aaaatagacc ataggcaata gattaacaat taatataata
      841 caagcaaata ttctattcat tgtatttctg ctttcaaaaa gttctaatat tgaaagtata
      901 attccacaaa tgcctataat aagtatggga ataagattag aaaataacac cccgagccaa
      961 tctgtgaaaa ttactatatt tactaatatc cctattaaca tacctatcat atttattaaa
     1021 tcaatttttt tcattagata tttctccttt attaaatctg aagtacttat attaaaataa
     1081 ttctaaacta tttacctcaa aaagtgcaat aaattatttc tgaacaataa aatgatagtc
     1141 aaaacaaaaa aatattgtta atcgttcaga gccatccgag agtgtaaaat attttgtgta
     1201 aatgaaaaaa tccatacaaa aaaggaaggc gcttctgtag aataaagtta acgacaacca
     1261 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
     1321 gtcactaaag gcg
//
LOCUS       DAFEHS010000171         1321 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFEHS010000171
VERSION     DAFEHS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.178"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(372..445)
                     /locus_tag="LP327_002800"
     tRNA            complement(372..445)
                     /locus_tag="LP327_002800"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Ile,seq:cat)
     gene            complement(1000..1314)
                     /locus_tag="LP327_002801"
     CDS             complement(1000..1314)
                     /locus_tag="LP327_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726844.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          488 a          209 c          195 g          429 t
ORIGIN      
        1 actaattaac taccactcca attataagtc tttttcccat tattttttac agttatatgg
       61 tatgcttttt aatggcttca aatataaaag agtttaaagc gtaacacact tatggggaag
      121 tggtttttgg ggaacgcttt aaactcttct ttattattat ctcacaatat aacccaaatg
      181 tctttccatt taaaaatcaa agtaaaactt ttcaaatata cagaagtata actatgtgaa
      241 acatcctttc attaatccat aaaaggatac ataaaaaagc cactcatttg agtggcaatg
      301 aagaaaagct ttagcttgta taatactctt caaaaaattc taacacagaa cgattcaatt
      361 ggctacatta atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg
      421 ctctgaccaa ctgagctaag ggtagtggtt gttgccacat aaagccataa acaatcaacc
      481 agtagaatgc gtggcaacaa acctgttatc gcatatcttg gagtgtgact atttatgagt
      541 gatagtgaag atatgcgtct ccatatatgt atggttgaga cagataaaca tgtatgaaaa
      601 acgttgttaa atcaacgttt ttttattttg gtcaaggaga gaaaagtaga gaaatacaaa
      661 attttttgca ttgattttgc attcaaaaaa acattcaaac atttagaaat ccccaatacg
      721 tattcaggaa aatctatttt aaggtttaca aaaagaactt ttgttcgtac actctttttg
      781 aggtgaactt ttatgctgat ggaaggaaaa acacaattat ggtttaagca tgttccttca
      841 aataagtttt ttaattataa actctagaga aattaattca tcctaacata aaataattgt
      901 tcactcaaaa gtttttctag aacaattaaa acagcaggct tcacattcgc tatacatttg
      961 taagaagtaa taattcctgt actaaaaggc ataattgctt taattatttt ttgacttctc
     1021 ttcaaacaca agaagtagtt gtgtaataat tgcagaaata aggattccaa taatagatat
     1081 aaaatttgac atggaaacgt ctataacatt aaaagaataa agtgttgaag aaagtaaact
     1141 gattgaaata acccctccgt aaaaaatagt ctgtgaatat acccactttt tttcagaaga
     1201 cgtacttcgt ttggttctaa aacctatccc tgcatttcta ctcttatcaa aaaaccaaat
     1261 atttagaagt aataatattg ctattattaa gataaaatta ctgtaaaaat ccaaaagcta
     1321 t
//
LOCUS       DAFEHS010000172         1318 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFEHS010000172
VERSION     DAFEHS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.179"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(62..280)
                     /locus_tag="LP327_002802"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="LP327_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="LP327_002803"
     CDS             complement(546..740)
                     /locus_tag="LP327_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726845.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="LP327_002804"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="LP327_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          468 a          218 c          180 g          452 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatat
//
LOCUS       DAFEHS010000173         1311 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFEHS010000173
VERSION     DAFEHS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.180"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            129..>1311
                     /locus_tag="LP327_002805"
     CDS             129..>1311
                     /locus_tag="LP327_002805"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL1726846.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
BASE COUNT          411 a          262 c          236 g          402 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagcag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac gctccttatt tctcactagt taaagagtgt tttccaaatg
      841 cacaagtgat cgtcgatcgg tttcacattg ttcaacatct gaaccgcttc ttcgatgcgg
      901 ttcgaaaacg tgtgatgaaa acacttgatc aaaaagaccc aatacaagct aaacagtatc
      961 gccaattgaa atccttattc aaattacttt taaaatcaga ggatcgttta gattacaata
     1021 cactaacgaa aagacgcaat ttcaattgga gactattgac tgaaacagaa gtagttgacc
     1081 gccttctaaa gatttctcct gaacttaaaa cagcttatcg ttactaccaa gacctgctta
     1141 ctgcgtatca ggaaaaagat ccagatactt ttttccagct gcttcgagca atgcctggag
     1201 aagttccatt ggaactgcac catatcaaaa aagccttttt caaatacgag aaagggattc
     1261 ggctggcatt actgaagaag tattcgaatg cacgactgga aaatctccat a
//
LOCUS       DAFEHS010000174         1310 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFEHS010000174
VERSION     DAFEHS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.181"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..1095)
                     /locus_tag="LP327_002806"
     rRNA            complement(<1..1095)
                     /locus_tag="LP327_002806"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          291 a          366 c          251 g          402 t
ORIGIN      
        1 cctagttgtc tgtgcaaccc cacatccttt tccacttaac atatattttg ggaccttagc
       61 tggtggtctg ggctgtttcc ctttcgacta tggatcttat cactcacagt ctgactcccg
      121 gatatgaatg aatggcattc ggagtttatc tgaattcggt aacccgagat gggcccctag
      181 tccaaacagt gctctacctc catcattctc aattccgagg ctagccctaa agctatttcg
      241 gagagaacca gctatctcca agttcgtttg gaatttctcc gctacccaca cctcatcccc
      301 gcacttttca acgtacgtgg gttcggtcct ccagtgcgtt ttaccgcacc ttcaacctgg
      361 acatgggtag atcacatggt ttcgggtcta cgactacata ctcaaacgcc ctattcagac
      421 tcgctttcgc tgcggctccg tctcttcaac ttaacctcgc atgcaatcgt aactcgccgg
      481 ttcattctac aaaaggcacg ccatcaccca ttaacgggct ttgacttgtt gtaggcacac
      541 ggtttcaggt tctatttcac tccccttccg gggtgctttt cacctttccc tcacggtact
      601 ggttcactat cggtcactag ggagtattta gccttgggag atggtcctcc cggattccga
      661 cggaatttct cgtgttccgc cgtactcagg atcctcctag gtgtcttcca catttcgtct
      721 acggggtttt taccctcttt gactgacttt tccaagtcat tcgactatct gaaagaacta
      781 ccatattgga gtcctacaac cccaacaagc aagcttgctg gtttgggctt ttcccgtttc
      841 gctcgccgct actcagggaa tcgaattttc tttctcttcc tgcaggtact tagatgtttc
      901 agttctctgc gtctacctcg aatgtgctat gtattcacac aatcgtaaca tcctattaaa
      961 gatgttgggt tcccccattc ggaaatctct ggatcatagc ttacgtacag ctccccaaag
     1021 catatcggtg ttagtcccgt ccttcatcgg ctcctagtgc caaggcatcc accgtgcgcc
     1081 cttattcact taaccttatc aaccttgcgg ttgggttttg atccttcttc tagcgataga
     1141 agttagatca agaaaataag caattgaact tattaaaaaa ctctatccag ctcacaaatc
     1201 agcctgtgcg gcaaaaagat cgatcgtctc ataaaatcaa agattttata gccgtccgcc
     1261 tattttgctt taggctaaac aatttgttca gctttcaaat tcaacgcggt
//
LOCUS       DAFEHS010000175         1305 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFEHS010000175
VERSION     DAFEHS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.182"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            96..506
                     /gene="spx"
                     /locus_tag="LP327_002807"
     CDS             96..506
                     /gene="spx"
                     /locus_tag="LP327_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL1726847.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLLKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            520..876
                     /locus_tag="LP327_002808"
     CDS             520..876
                     /locus_tag="LP327_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL1726848.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          467 a          186 c          243 g          409 t
ORIGIN      
        1 ttattttgat ttttttttat attttattag ctaataattg gtattttaaa agaaattact
       61 gtagaattgg attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta
      121 gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag
      181 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagacc ttggcagcga
      241 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
      301 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attgaaagaa tatccttcat
      361 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
      421 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
      481 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
      541 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
      601 tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc
      661 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
      721 attgcgattg acctttcctg tgtagaaaaa aaacaactga tttttttatg cacacaacat
      781 accaacctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
      841 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
      901 ctgagcattc catacagttg taacagtcga tcgaacactt agcatcaagc gtgttatgtg
      961 ttcttttttt gactttattt ttctactata gagacatgct caatttatgt ggtcaaaatt
     1021 caaatctagg tggggatata gagttaaaaa tatattgaca tgtgtaaatt tacatgatat
     1081 attcatttct gttgttttaa gtagcaaagc aataaaatag tattttaaaa aacatataaa
     1141 atagttgttg acatgttaga aatgacatga taagataact gagtcgttaa aaaaggaccg
     1201 cttgcgaagc ttgattctat cgaaaaaact tcaaaaaagt tcttgacaaa taacaaacga
     1261 caagttatga taaatgagtt gctgtaagat ttcgaaaaac gaaat
//
LOCUS       DAFEHS010000176         1294 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFEHS010000176
VERSION     DAFEHS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.183"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..1244
                     /locus_tag="LP327_002809"
     CDS             <1..1244
                     /locus_tag="LP327_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL1726849.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAFEHS010000177         1274 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFEHS010000177
VERSION     DAFEHS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.184"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            210..>1274
                     /gene="scm"
                     /locus_tag="LP327_002810"
     CDS             210..>1274
                     /gene="scm"
                     /locus_tag="LP327_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL1726850.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT          457 a          216 c          234 g          367 t
ORIGIN      
        1 ttatttttta cactttaaca gtcaccatca tcaatagttt ggagattgaa agaaaaagat
       61 agtgtcgaac tatcaattga acaagaagca acaatatgaa cgtttcatta atattcttta
      121 aataaacaat gatttttcaa acgaacagtt taagactagg agaaaaagag tataatagga
      181 aaatgtttga aaggaaagga aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag
      241 ttgctttgtt gttcaatatc ttttcgtatg gtgtggccgt ttcagcggaa acatacattc
      301 ctaaagaccc tgggaatatt tataatgatc taactggtaa ctatggcttg ttaggaattg
      361 ctagtcaatt ccacgtattt gctaaaaata agacaactgt gaatgcacat acagacggaa
      421 atatagcaac acatgaactt gatgcaaaaa ataattttgg tacagagctt catgaaggtt
      481 tattgaacca agaaataaat tatatacaaa aaactgattc tttcgctaat tcttcaggca
      541 tacctacagg tagcacaaga atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac
      601 ttcaagatgg tcatcctaca gtcaatggat ccaggcttga tcacttaact ccagatgatt
      661 tttataaaga tcgtgctggg catgaatata ttgatttcga gcaggaattt gctaaattga
      721 atgtagcagc aaatgatctt gcaacgatca ctccagctaa aacatatact gctgcagatt
      781 ttccagacat gaacaatcgg acaatcgatt taactgattt atcagatacc gggtttttat
      841 tggtaaatat cgatgcagaa gtgttaacga tgaatacgcc acttcaaatc attaatccaa
      901 atgatcaagt agttgtattt aatgtcatca acagttcttc tgcattgaac gtacagtcac
      961 caatcaaata caatgatcgt tcaaatcatg aaacagaaga tttttcggat gcgaatatct
     1021 cttggaattt tggaaatgag atgactgatt tgacaatcag tgctcctttt caaggaacaa
     1081 tccttgcgcc taatgctact atccgcatta ctcaaaacca agacggaacg attataggaa
     1141 caaatgtgat tttagatgca gctaccaatc gttgggatcc taacgaaata tttatcagcg
     1201 acaatggtac agatacgaca gacacaagcg acagtacaga tacatctacc acaagtgata
     1261 gctcagattc atca
//
LOCUS       DAFEHS010000178         1091 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFEHS010000178
VERSION     DAFEHS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.186"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="LP327_002811"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="LP327_002811"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL1726851.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DAFEHS010000179         1081 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFEHS010000179
VERSION     DAFEHS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.187"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(493..>1081)
                     /locus_tag="LP327_002812"
     CDS             complement(493..>1081)
                     /locus_tag="LP327_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL1726852.1"
                     /translation="FISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSKDYK
                     EVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSAIRK
                     IMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAMVLN
                     QLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          355 a          201 c          173 g          352 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 a
//
LOCUS       DAFEHS010000180         1061 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFEHS010000180
VERSION     DAFEHS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.188"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(36..992)
                     /locus_tag="LP327_002813"
     CDS             complement(36..992)
                     /locus_tag="LP327_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726853.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DAFEHS010000181         1032 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFEHS010000181
VERSION     DAFEHS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.190"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..899
                     /locus_tag="LP327_002814"
     CDS             <1..899
                     /locus_tag="LP327_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL1726854.1"
                     /translation="QVANNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGG
                     IVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGI
                     PLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPT
                     TSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIM
                     DYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPE
                     GNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          356 a          192 c          206 g          278 t
ORIGIN      
        1 cacaagtagc aaataatcag tatattttac tgcatgaaac ggctaatgaa acagcaacag
       61 gacgcaatga agcgcagtat atgcaacgtt catggactag cgcttatact gcttatattg
      121 tgggagacgg cggaattgtt tatcaagtcg gtcaacctgg ttatgtacag tacggtgctg
      181 gttcgtatgc taatgccaac agtcctgtgc agattgagtt acaacacaca catgataaag
      241 caacgtttga gaaaaactac aaggcatacg ttgaattggc tagagattca gcaatgaaat
      301 atggtattcc attaacgttg gacactcctt ataaccaacc gggaatcaaa tcgcatttat
      361 gggtaacaca aaacatctgg ggcgatcata cagatcctta cggttatctt tctgaaatgg
      421 gcgtaagtaa agaaaaatta gcatatgatt tagctcatgg atttaccgat gaaaatccaa
      481 caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct aatcctacac
      541 tgacagatgg aacgaattac gcccacattg atcagtttgg agaaatcgaa aatgcaaatt
      601 tgcatgtagc tggatggcac attgctaact ataaatacga gtatatcttc attatggatt
      661 acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga tcagatgtaa
      721 atcaagctta taatacttct ggaaatgttg gctatcatgt atcttttaac atgcgtaact
      781 tccctaataa gaaagtctat gtcatgatgc gggcaacgaa tgatccagag ggaaacacta
      841 aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct aaacgataaa
      901 aatagctcct cgttgaggag cattacataa ctatattgac aactataaaa atcattcgat
      961 aaaatagtga tgttatcgca tatcttcact atcacccata atagtcacac tccaagctat
     1021 gcgataacag gt
//
LOCUS       DAFEHS010000182          970 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFEHS010000182
VERSION     DAFEHS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.191"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            65..>970
                     /locus_tag="LP327_002815"
     CDS             65..>970
                     /locus_tag="LP327_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL1726855.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          346 a          172 c          168 g          284 t
ORIGIN      
        1 gttggtaaat caacaaaaaa acgcatatag tagtctcaaa actcccaaga aatgaggact
       61 acatatgcgt tcatcaaaat atactgaaca ttatacagat acttttataa cattcaagga
      121 aattatgaga actgtttcta acatatacca caattgtgta ccagataaga ttaaaaaccg
      181 aagaaatact gatcaactga agcaacgcga tacagtgatt attgcttgtg ttatatgggg
      241 cataattaat ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt
      301 tcctaatggt gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta
      361 cactatcaag attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg
      421 aattatagat agttttccta gtccattatg taaaccagtt agaaatagac aagcaaaact
      481 attgaatcaa atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct
      541 taaaattcat atgatcgtta ctaagactgg ctttccgatt acttactcaa tcacgaatcc
      601 aggtgtccat gacgtgaaag tgttagagac tttatcggaa gaagcgaatc ttcccaatat
      661 cctaggggac aaaggatata ttagccataa aatccatgaa aagcttgccc ttaagggcat
      721 tactatatcc gttccgccac gtaaaaacat ggataaatca gaaaaactag accacagctt
      781 acttggaaaa caaagaaaaa cagttgagac tgttttttct tccttagaaa aattaggttg
      841 tcaaaatttc aactcacgtt ctgttaaagg gctagagagt agatttgaaa gcatattact
      901 ggcttacagt gttctattaa gtcgagcaca acgacgtttt gaaggaactt tgagatattc
      961 tttaggatat
//
LOCUS       DAFEHS010000183          967 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFEHS010000183
VERSION     DAFEHS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.192"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            8..538
                     /gene="sat4"
                     /locus_tag="LP327_002816"
     CDS             8..538
                     /gene="sat4"
                     /locus_tag="LP327_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL1726856.1"
                     /translation="MKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEAPYLK
                     SYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDIAVCK
                     DFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDTMLYA
                     NFENNFEKAVFWYLRF"
     gene            631..>967
                     /locus_tag="LP327_002817"
     CDS             631..>967
                     /locus_tag="LP327_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBL1726857.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKII"
BASE COUNT          346 a          153 c          230 g          238 t
ORIGIN      
        1 tacagaaatg aaagcagggc acctgaaaga tatcgataaa cccagcgaac catttgaggt
       61 gataggtaag attataccga ggtatgaaaa cgagaattgg acctttacag aattactcta
      121 tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat gaagaggatg aggaggcaga
      181 ttgccttgaa tatattgaca atactgataa gataatatat ctttactacc aagacgataa
      241 atgcgtcgga aaagttaaac tgcgaaaaaa ttggaaccgg tacgcttata tagaagatat
      301 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      361 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      421 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      481 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      541 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      601 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      661 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      721 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      781 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      841 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      901 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      961 attatcg
//
LOCUS       DAFEHS010000184          963 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFEHS010000184
VERSION     DAFEHS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.193"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..704
                     /locus_tag="LP327_002818"
     CDS             <1..704
                     /locus_tag="LP327_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HBL1726858.1"
                     /translation="KKALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEA
                     KTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAV
                     LVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAE
                     STYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQR
                     ILDNELGCANASEAV"
BASE COUNT          321 a          172 c          201 g          269 t
ORIGIN      
        1 taaaaaaagc cttatcaaaa cgaggcattc agatcagccg acgaaaaatt agtagaatca
       61 tgaaaaatag aggattaaaa tcgagctata ctgttgctta ttttaaagta catcattcta
      121 cttgcaatga agccaaaacg acaaacgtat tgaatcgtaa attcttaaga gacaacccat
      181 tagaagcgat cgtaacagac ttgacttatg tacgagtcgg gaaaaaatgg aattatgtct
      241 gtttcatttt ggatctgttc aatcgagaaa ttctcggcta ttcttgtgga gaacataaag
      301 atgccgttct agtaaaaaaa gcatttagcc gtatcaaaca acctctgaca gaggttgaga
      361 tttttcatac tgatcgtgga aaagagtttg ataaccaagc tattgatgaa ttattaacaa
      421 cttttgacat caatcgatca ttgagtcata aaggctgtcc ttttgataat gccgtagctg
      481 aatcaactta taagtcgttg aaagtagaat ttgtctatca atacacattt gaaaccttac
      541 aacaattgga tttggagtta tttgactatg tcaattggtg gaaccacctt cggttgcacg
      601 gtacacttgg ctacgagaca ccggttggtt accgtaacca gagattggcg cagcgaatcc
      661 ttgataatga gctcggatgt gctaacgcta gcgaggcagt ctaactttaa ctgttagctc
      721 ctgccgaaga tcgtcacatc cgaggaggct cattgtcaag gacaatcgga atagcatacg
      781 gaaagaagtt ctgcacctta taaaatttgt caaaaaaact gttgccattc caatgagaaa
      841 aagcatggaa tgcgctgcat caggtatcga ggcttagaaa aaaatcaggt gcatacgatg
      901 cttgtttttg ccgaaatgag tctgaaaaaa ttggtcactt agcagtggga gcatatgtct
      961 tca
//
LOCUS       DAFEHS010000185          959 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFEHS010000185
VERSION     DAFEHS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.194"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            249..929
                     /locus_tag="LP327_002819"
     CDS             249..929
                     /locus_tag="LP327_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBL1726859.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRDINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          347 a          145 c          177 g          290 t
ORIGIN      
        1 agaattataa tcacaaactt aatagtaatt actattggga ttacaacatt aacaaataaa
       61 gctttaatat caagtatgat tttaatagtg tttgatttta ttatcattta tagtttgctg
      121 tttttaataa agaaagtaac tgaatattta aatcgttagg cacgaaataa atttggttct
      181 gttgcaaagt tttaaatcta ctatcaaata aggtagaata atagaaaaag atagcaggag
      241 gaatgacgat gaatcatttt aaaggaaagc aatttcagca ggatgtgatt attgtagccg
      301 tgggctacta tcttcgttat aaccttagct atcgtgaagt tcaagaaatc ttatatgatc
      361 gtgacattaa cgtttctcat acgacgattt atcgttgggt gcaagaatat ggcaaactac
      421 tctatcaaat ttggaaaaag aaaaataaaa aatcctttta ttcatggaaa atggatgaaa
      481 cgtacatcaa aattaaagga aaatggcatt atttgtatcg agccatcgat gcagatggtt
      541 taaccttgga tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta
      601 agcggttagt gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta
      661 ttacaagtgc ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta
      721 ccattaaata cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata
      781 aattctatcg aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag
      841 gattatataa gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa
      901 tcaaggtatt attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DAFEHS010000186          906 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFEHS010000186
VERSION     DAFEHS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.195"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..767
                     /locus_tag="LP327_002820"
     CDS             <1..767
                     /locus_tag="LP327_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL1726860.1"
                     /translation="IKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYISLLCKEE
                     IIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKN
                     KGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGML
                     RNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLE
                     IREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          335 a          144 c          164 g          263 t
ORIGIN      
        1 tcatcaaagt tcctaaattg aagtcattag taagaattaa gcttcataga caaccaaaag
       61 ggattatcaa atctgctaca atatcacgtc attctagcgg taaatactat atttctttgt
      121 tgtgtaaaga agaaattatc gaattaccta aaactaattc cacaattggt attgacttag
      181 gtattatgga ctttgcgatt ctttctgatg gacaaaaaat tgataataat aaattcacgt
      241 ccaaaatgga aaagaaacta aagcgtgaac aacgcaaatt gtctaggcgt gctttattgg
      301 ctaaaaataa aggtaccaat ctatttgaag ctaaaaatta tcaaaaacaa aaacgcaaag
      361 tagctagatt gcatgaaaaa gtaatgaatc aacgtactga ttttctgaat aagttgagta
      421 cagaaatgat caaaaatcac gatattatct gtattgaaga cttaaacaca aaaggtatgt
      481 tgcgaaatca taaattagct aaaagtattt ctgatgtttc ttggtcaaag tttgtgacta
      541 aattacaata caaggctgac tggtatggac gaaaaatcat caaagtagat aaatggtttc
      601 cgtctagtca gatttgttca gagtgcggac ataaagatgg caagaaatct cttgaaattc
      661 gagaatggac ttgtcctgtt tgtcatactc atcatgatcg tgatatcaat gctagtatca
      721 atattctgac cgaaggtcta agactacatt ctatgggatt ggcttagatc caatcatcaa
      781 gaaccgtagg aactacgggg atagcttggt aaataagaga caccgctgtt agtaaagaaa
      841 tacgctatca agtatgctct attcccaaga agctcccact tcaagcgtta agaaccgtca
      901 ggttta
//
LOCUS       DAFEHS010000187          830 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFEHS010000187
VERSION     DAFEHS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.197"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..792
                     /locus_tag="LP327_002821"
     CDS             <1..792
                     /locus_tag="LP327_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL1726861.1"
                     /translation="EFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFS
                     LATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRL
                     KGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPN
                     LRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHY
                     SYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          263 a          164 c          160 g          243 t
ORIGIN      
        1 gaattcaaat cggttcgaag tgttgccagt gcaatgagtt ttatcatgat ggacggacaa
       61 agccatgcat tattggatat tgtcgaaaat cgacgcttgc cttatttaga acgctatttt
      121 tctcggtttt cattagcgac tcgtgaagcc gttcaattga ttgttattga tatgtatgcg
      181 ccttatgttt cgttggtgaa aaagcttttc ccgaatgctc agttgatcat cgatcgtttc
      241 cacatcgtcc aacatattgg cagaactttc ttgaatcatc gagtgaaaga aaccacggta
      301 cgacttaaag gaaattccca ctctcaacga gggttaggga aaaaattgaa gcgctattgg
      361 aaactcctac aaaaagaaga agcaaagtta aactatgaaa aacgggtttg gcgtgccagt
      421 tttaaagatt atttgactga aagtgagatt gccgatcgac tgttagctgc ttgtcccaac
      481 ttacgccaag gctatcagtt ataccaagac attctttatg cggtaaagaa aagagatcag
      541 tcactatttg aggattgttt aactagagac attagcggtc tgccagaaac ctatacaacc
      601 actttacgaa cgtttaaaaa gtttctgcca cagatccaaa atgcgcttca ctacagctat
      661 tcaaacggac cattggaatg cttaaacaac cacatcaaag tattaaaacg caatgcgtac
      721 ggctttcgca acttttacaa tttcaaacta cgaattatga ttcgtcatgg caaaacattt
      781 ttaaccaaat aaacagaaca gaaagaggtt ttcttctttc tgttctgtcc
//
LOCUS       DAFEHS010000188          789 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFEHS010000188
VERSION     DAFEHS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.198"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..782
                     /locus_tag="LP327_002822"
     CDS             <1..782
                     /locus_tag="LP327_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL1726862.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          106 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgccc
//
LOCUS       DAFEHS010000189          763 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFEHS010000189
VERSION     DAFEHS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.199"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(237..716)
                     /locus_tag="LP327_002823"
     CDS             complement(237..716)
                     /locus_tag="LP327_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726863.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKNIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          261 a          102 c           85 g          315 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttatattt
      481 ttacctttgt aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata
      541 tgtatgtcaa tatttttcat ctgtttcata ggtatttcaa catctaaact attaaaaaca
      601 tataattccc tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat
      661 cctccaccca ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta
      721 agctcagttt gtttatcatc ctcaataatt ttataaatca aac
//
LOCUS       DAFEHS010000190          756 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFEHS010000190
VERSION     DAFEHS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.200"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(66..>756)
                     /locus_tag="LP327_002824"
     CDS             complement(66..>756)
                     /locus_tag="LP327_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HBL1726864.1"
                     /translation="LETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEE
                     QNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIE
                     SFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLL
                     EDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENG
                     RKEIYKRTYKD"
BASE COUNT          255 a          124 c           50 g          327 t
ORIGIN      
        1 tttttcattt ttttaaacta tttaatacta atgtctttta taatagcttt tcatatatta
       61 tataatcaat ctttataagt ccttttataa atttcttttc taccattttc gataaattcc
      121 tgtttaatat ttttaattcc ataaacaata gtttcaatag gataatattc ttcaactata
      181 tcttgatatt cttttgcttt ctcaatatct atatttccat acattcttaa tatatcttct
      241 ccaaaatttg ttcctatttc ttcttcacta tcttcaagta agtatataaa atcacaatat
      301 tcatctataa ttccagaatc tccaaaatca attattccag ttaatctatt attgccatct
      361 aacaatagat gattacaact aaaatcatta tggcataaac actttttacc ctcaaaaact
      421 gttgttgcat ttagtctttc cataaaactt tctatataat ctttttctat atcagttaaa
      481 tcattataaa tagtttcacg caacaatata tactcttcta atacattttg tttattatca
      541 atagtacatt cactaatatc tgtataatct aaaccgtgca tttgtcttaa aaaactggca
      601 atatctcgtt ttaacaaatt ttgttcttct tctgacatag tagaataaat ttctggtgtt
      661 aaaaaagttc ctttaatttc tttataacct agtatagata attcatcact aatatacgaa
      721 tattcaatat taggaatttt tacattagtt tctaaa
//
LOCUS       DAFEHS010000191          731 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFEHS010000191
VERSION     DAFEHS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.201"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LP327_002825"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LP327_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBL1726865.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="LP327_002826"
     CDS             complement(481..675)
                     /locus_tag="LP327_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726866.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DAFEHS010000192          692 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFEHS010000192
VERSION     DAFEHS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.203"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..446)
                     /locus_tag="LP327_002827"
     CDS             complement(<1..446)
                     /locus_tag="LP327_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726867.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          207 a          118 c           92 g          275 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata tt
//
LOCUS       DAFEHS010000193          689 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFEHS010000193
VERSION     DAFEHS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.204"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            82..>689
                     /locus_tag="LP327_002828"
     CDS             82..>689
                     /locus_tag="LP327_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HBL1726868.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT          230 a          119 c          135 g          205 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaatt
//
LOCUS       DAFEHS010000194          641 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFEHS010000194
VERSION     DAFEHS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.205"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(83..514)
                     /locus_tag="LP327_002829"
     CDS             complement(83..514)
                     /locus_tag="LP327_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBL1726869.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          220 a           83 c           72 g          266 t
ORIGIN      
        1 ttttttagga atgtaaagtc ttatctcttt tcttaattgt acaattagta ttatctgcac
       61 tactttctaa ttaaataaat ttttagttat taacttgatt aaaatcagag ataatatcat
      121 ttaattcagt ttgaagggtt gtaacgatta aatggaaatt ttcttcatct gttttattat
      181 atggggatgt tttcgttata tataacaact tttctttttc aattttcatt aataactctg
      241 tcgaagtctg tactttatct tgataattaa atgcagacat accactttgt ataattgtaa
      301 ttattgctga agcaattgaa acaatcaata gtaaagacct gtgttcggaa gcctgatcaa
      361 ttaatattgg gatactagct gataagacta tttttataat atttccgata ccaattactc
      421 tattataaaa tcgaatttct ttttttaatc tatcgattgt agaatcaata gattcgataa
      481 acgctttttg atccatcatt ttctctccaa tcattttatt aaaatcatta acaaaaatat
      541 tataatagga tttaagtaag ttctaactaa tcacatataa aaataaaaaa acatttgtat
      601 tttatcaaag ttttattata cttgtcaatt tgtgtacatc c
//
LOCUS       DAFEHS010000195          618 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFEHS010000195
VERSION     DAFEHS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.206"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..>618
                     /locus_tag="LP327_002830"
     CDS             <1..>618
                     /locus_tag="LP327_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1726870.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTN"
BASE COUNT          261 a           53 c          106 g          198 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaaatt
//
LOCUS       DAFEHS010000196          597 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFEHS010000196
VERSION     DAFEHS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.207"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(310..>597)
                     /locus_tag="LP327_002831"
     CDS             complement(310..>597)
                     /locus_tag="LP327_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726871.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          206 a           98 c           90 g          203 t
ORIGIN      
        1 attgtgagat gataataaag aagagtttaa agcgcacccc aagccacttc cccataagtg
       61 tgttacgctt taaactcttt tatatttgaa gctattaaaa ggcataccat atttttgaaa
      121 aaaagtgaga aaaaaggctt acaattggag tggtagttaa ttagtgactt atttttgatt
      181 ttatagcact gatactataa aatatagata tcatcatatt acacaatctt aatactaact
      241 taaaaaatat ctcctttcac aagtatggtg ataaaattcg ttccgggcta cctttttagg
      301 tagcctactt taatctttgt atctttctgg atcaacgaaa gtatacttta tatagtcata
      361 acgccgatga tcgctacgtg cgtccggcac gtcagtcacg atatcaaaca aaaagtatac
      421 atccttcttc attctagttt tcgcagcagg aattttgaaa tagttcttat tagaatagta
      481 gagattgatt aataagctat cttcgattgc taaaaagaaa acttctgaat tccatacttt
      541 ataaaaatct ttgataaatc tattcgaagg gtcaaattta aaccataatt gtgtctc
//
LOCUS       DAFEHS010000197          578 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFEHS010000197
VERSION     DAFEHS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.208"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(103..>578)
                     /locus_tag="LP327_002832"
     CDS             complement(103..>578)
                     /locus_tag="LP327_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL1726872.1"
                     /translation="RGYNSTKNAYFYGLKIHIIVTKTGFPVTYSITDPSVHDVKVLET
                     LSEEANLPNILGDKGYVSHKIHEKLALKGITVLVPSRKSMDKLGKIDHRLLTRQRKAV
                     ETVFSSLERLGFQNFKSRSIQGLESKFESILLSYSILLSRAQQHFEGTLRYSLGY"
BASE COUNT          176 a          106 c           94 g          202 t
ORIGIN      
        1 acatttccat ttcatttacc tctttttcgt ttaattatac taaagttcta tccaagcgac
       61 aagctgtgta taataaaaat atacccgtgg tgctagttaa ttttaatacc ctaaagaata
      121 tctcaaagtc ccttcaaaat gttgctgtgc tcggcttaaa agaatactgt acgataataa
      181 tatgctttca aacttacttt ctagcccttg aatagaacgt gatttgaaat tttgaaaacc
      241 tagtctttcg agagaagaaa aaacagtttc tactgctttt ctttgtctag taagtaatcg
      301 atggtctatc ttccctagtt tatccatgct ttttcgtgac ggaactaaga ctgtaatgcc
      361 cttaagggca agcttttcat ggattttatg gctgacatag cctttatctc caaggatatt
      421 tggaaggttt gcttcttccg ataaagtctc taaaactttc acatcatgaa cactaggatc
      481 cgtgattgaa taagtaactg gaaagcctgt tttagtgacg ataatatgaa ttttaagacc
      541 ataaaaataa gcgtttttcg ttgagttata gcccctac
//
LOCUS       DAFEHS010000198          577 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFEHS010000198
VERSION     DAFEHS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.209"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            312..>577
                     /locus_tag="LP327_002833"
     CDS             312..>577
                     /locus_tag="LP327_002833"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL1726873.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLST"
BASE COUNT          196 a          106 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccac
//
LOCUS       DAFEHS010000199          574 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFEHS010000199
VERSION     DAFEHS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.210"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..572
                     /locus_tag="LP327_002834"
     CDS             <1..572
                     /locus_tag="LP327_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBL1726874.1"
                     /translation="IRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIK
                     GRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQY
                     QMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLN
                     EYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          248 a           71 c           94 g          161 t
ORIGIN      
        1 aaatccgaat aaagcgatat aaatcttata aaggaacagt aggaaaaatt gccaagaatg
       61 tgttaaaacg aaatttttca gtagatacac ccaataaaaa atgggtgaca gacgtcactg
      121 aattcaagat aaaaggaaga aaaatctatc tatctcctat acttgattta tttaacggag
      181 aaataattag ttatagtata tcaactagtc caacatataa actgattgaa gaaatgcttc
      241 aacaagcaat taaaaaaaag ggaactgagg gttcactgat tctacattca gatcaaggat
      301 ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga gaataacatt attcaaagca
      361 tgtctagaaa aggaaattgc ttagataatt ctgtaataga aaattttttt ggtgtgctta
      421 aatcagagtt tttttatcga gaaaaattcc gatcaattga gatatttcaa agtaaattaa
      481 atgagtatat taggtggtat aataacaaaa ggataaaact aaagttaaat ggactatctc
      541 cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAFEHS010000200          573 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFEHS010000200
VERSION     DAFEHS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.211"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            77..>573
                     /locus_tag="LP327_002835"
     CDS             77..>573
                     /locus_tag="LP327_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBL1726875.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          214 a          106 c           93 g          160 t
ORIGIN      
        1 aatccataca aaaaaggaag tcccttctgt agaataaagt taacgacaac caattcacag
       61 aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc tagtcactaa
      121 aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca cactcctacg
      181 gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt ttaattcagg
      241 taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg aattaacatt
      301 ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag cctacaaaag
      361 aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg ttacgatgtc
      421 tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac aaaccatgtc
      481 caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag ccttaaatga
      541 taagtatgtc gctattttta tggacgctac tta
//
LOCUS       DAFEHS010000201          563 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFEHS010000201
VERSION     DAFEHS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.212"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
BASE COUNT          217 a           75 c          117 g          154 t
ORIGIN      
        1 cttattatca gttgaaagaa caaataataa ataaagggaa aagatatgga atagaagtta
       61 aaagtgagta gaataagaaa tcgttgattt ggcaacgaaa tagtgagggg acactcactc
      121 actgaagggt tagctctgtc aagttgtgta accagacgga aggacgaata ttgttgaggt
      181 tttagaacca tgtaaaacaa cacagttctc aaatgttacg gataaaccgt ttgcgggaaa
      241 tcaggtttta aaaccatgta aaacaacaca gttctcaaat aaggctttat tgaattgatc
      301 agtagtttct gttttagagt tatataaaac gacatggctc ttaaatagga gctaacgaag
      361 aaaaagaagg tactccgttt tggagttaag tagaaataaa aaaatagaat cgttgatttg
      421 gcaacgagtt gaaaaatacg caaaactatg gtcaccaagg gataaccctg aaactgatga
      481 tcgctatatc ttactagtag ttaaacagtc taaatagtac tcatttattg tattgattct
      541 aagggaacct gcaataaatg agt
//
LOCUS       DAFEHS010000202          561 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFEHS010000202
VERSION     DAFEHS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.213"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            75..521
                     /locus_tag="LP327_002836"
     CDS             75..521
                     /locus_tag="LP327_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL1726876.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT          190 a           88 c          130 g          153 t
ORIGIN      
        1 attcgtaagg ttcgtttgct tttttgtgtg cgaatcggta tgctaagaat aaaaaagcag
       61 ggggcgtttg cttagtggaa tatcaggtaa agaaatttga agaattgtca acagcagagt
      121 tttacgcgat cgtcaaagaa cgaatcgcag tttttgttgt ggaacaacag tgtccttatc
      181 aagaagttga tgacattgat ggacaagctt ggcatacgtt ttttcaagat actgatggga
      241 agatccttgc ctacacgcga gtatatgaag aagcggagag tatccatttt ggacgtgtcc
      301 ttgttcatca ggacaaccgt aaagatggtt taggaagaaa gattgttcaa gaaacgatta
      361 acatgatcca gaaaaatttt ccagaaaaac caatcgtgat cggcgcacaa gaatacctac
      421 aagcttttta tgagtcgttt ggattcaagg caatctcaga tgtgtatttg gaagatgata
      481 ttccacatat cgacatgaaa aaggaacaag tagcaagtta agaatctaaa aaaagaaagc
      541 cgacactgaa aatcggcttt c
//
LOCUS       DAFEHS010000203          548 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFEHS010000203
VERSION     DAFEHS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.214"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..>548)
                     /locus_tag="LP327_002837"
     CDS             complement(<1..>548)
                     /locus_tag="LP327_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL1726877.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DAFEHS010000204          545 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFEHS010000204
VERSION     DAFEHS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.215"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            141..398
                     /locus_tag="LP327_002838"
     CDS             141..398
                     /locus_tag="LP327_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726878.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          232 a           68 c           76 g          169 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttac
//
LOCUS       DAFEHS010000205          528 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFEHS010000205
VERSION     DAFEHS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.216"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(5..90)
                     /locus_tag="LP327_002839"
     tRNA            complement(5..90)
                     /locus_tag="LP327_002839"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="LP327_002840"
     tRNA            complement(107..178)
                     /locus_tag="LP327_002840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="LP327_002841"
     tRNA            complement(190..262)
                     /locus_tag="LP327_002841"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="LP327_002842"
     tRNA            complement(280..361)
                     /locus_tag="LP327_002842"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="LP327_002843"
     tRNA            complement(369..441)
                     /locus_tag="LP327_002843"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="LP327_002844"
     tRNA            complement(445..517)
                     /locus_tag="LP327_002844"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAFEHS010000206          525 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFEHS010000206
VERSION     DAFEHS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.217"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..134
                     /locus_tag="LP327_002845"
     CDS             <1..134
                     /locus_tag="LP327_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL1726879.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          220 a           75 c           56 g          174 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc atacaaacaa aaataaataa agtcggattt ttctgataat
      181 tcaacgcaaa aaaacgaaca atttttcaat aaatcagtct tctaaaatag ataacctaaa
      241 aaaaacgaac aaacaaataa aatgactgtc cttcattcac taaacatatt tttatttttt
      301 atattgttaa aaattcacaa ttatttttta atagaattat ctgttttcca aattttataa
      361 cataaaaaac cttttttaac ggcatttata tgctttttca gaaattaact gatgatttca
      421 aaaagaaaac atgtcataat atttcccata ttgtgcaaaa aaatacaagg tggaggctct
      481 actatgattg aagaatatat tgagaaaaat attttaagac agctg
//
LOCUS       DAFEHS010000207          522 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFEHS010000207
VERSION     DAFEHS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.218"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            62..>522
                     /locus_tag="LP327_002846"
     CDS             62..>522
                     /locus_tag="LP327_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL1726880.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRP"
BASE COUNT          161 a          105 c           99 g          157 t
ORIGIN      
        1 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
       61 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      121 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      181 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      241 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      301 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      361 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      421 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      481 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca tt
//
LOCUS       DAFEHS010000208          513 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFEHS010000208
VERSION     DAFEHS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.denovo.219"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(107..>513)
                     /locus_tag="LP327_002847"
     CDS             complement(107..>513)
                     /locus_tag="LP327_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBL1726881.1"
                     /translation="TPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNA
                     ASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          102 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttc
//
LOCUS       DAFEHS010000209        23715 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.guided.037, whole genome shotgun sequence.
ACCESSION   DAFEHS010000209
VERSION     DAFEHS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.guided.037"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..212
                     /locus_tag="LP327_002848"
     CDS             <1..212
                     /locus_tag="LP327_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL1726882.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
     gene            447..683
                     /locus_tag="LP327_002849"
     CDS             447..683
                     /locus_tag="LP327_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726883.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="LP327_002850"
     CDS             690..2642
                     /locus_tag="LP327_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBL1726884.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3198)
                     /gene="dfrG"
                     /locus_tag="LP327_002851"
                     /pseudo
     CDS             complement(2714..3198)
                     /gene="dfrG"
                     /locus_tag="LP327_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
     gene            3560..4600
                     /locus_tag="LP327_002852"
     CDS             3560..4600
                     /locus_tag="LP327_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL1726885.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4698..4886
                     /locus_tag="LP327_002853"
     CDS             4698..4886
                     /locus_tag="LP327_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL1726886.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4944..5297)
                     /locus_tag="LP327_002854"
     CDS             complement(4944..5297)
                     /locus_tag="LP327_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726887.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5828..6250
                     /locus_tag="LP327_002855"
     CDS             5828..6250
                     /locus_tag="LP327_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL1726888.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6247..6477
                     /locus_tag="LP327_002856"
     CDS             6247..6477
                     /locus_tag="LP327_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726889.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6975..7175
                     /locus_tag="LP327_002857"
     CDS             6975..7175
                     /locus_tag="LP327_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL1726890.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7202..8395
                     /locus_tag="LP327_002858"
     CDS             7202..8395
                     /locus_tag="LP327_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL1726891.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8463..10028)
                     /gene="guaA"
                     /locus_tag="LP327_002859"
     CDS             complement(8463..10028)
                     /gene="guaA"
                     /locus_tag="LP327_002859"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL1726892.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10087..11010)
                     /locus_tag="LP327_002860"
     CDS             complement(10087..11010)
                     /locus_tag="LP327_002860"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL1726893.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11206..11811
                     /locus_tag="LP327_002861"
     CDS             11206..11811
                     /locus_tag="LP327_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL1726894.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12119..13135
                     /locus_tag="LP327_002862"
     CDS             12119..13135
                     /locus_tag="LP327_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL1726895.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13276..14577
                     /locus_tag="LP327_002863"
     CDS             13276..14577
                     /locus_tag="LP327_002863"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL1726896.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14623..15285
                     /gene="deoC"
                     /locus_tag="LP327_002864"
     CDS             14623..15285
                     /gene="deoC"
                     /locus_tag="LP327_002864"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL1726897.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15306..15704
                     /locus_tag="LP327_002865"
     CDS             15306..15704
                     /locus_tag="LP327_002865"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL1726898.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15770..16852
                     /locus_tag="LP327_002866"
     CDS             15770..16852
                     /locus_tag="LP327_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL1726899.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17064..18626
                     /locus_tag="LP327_002867"
     CDS             17064..18626
                     /locus_tag="LP327_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL1726900.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18619..19752
                     /locus_tag="LP327_002868"
     CDS             18619..19752
                     /locus_tag="LP327_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1726901.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19752..20708
                     /locus_tag="LP327_002869"
     CDS             19752..20708
                     /locus_tag="LP327_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL1726902.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20807..21973
                     /gene="deoB"
                     /locus_tag="LP327_002870"
     CDS             20807..21973
                     /gene="deoB"
                     /locus_tag="LP327_002870"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL1726903.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21997..22815
                     /locus_tag="LP327_002871"
     CDS             21997..22815
                     /locus_tag="LP327_002871"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL1726904.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22832..23539
                     /gene="deoD"
                     /locus_tag="LP327_002872"
     CDS             22832..23539
                     /gene="deoD"
                     /locus_tag="LP327_002872"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL1726905.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         7716 a         4198 c         4946 g         6855 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttggaatgt cattctcttt gccaatcact ctattcttat ccatcgcagc
     3181 aatcaaagaa actttcattt ataacacagc ctttttaact aatttaccat ctaatttctt
     3241 atttctatca aaaaaccttt tctcattatc ctgcccgtta gttgaataag caattttttc
     3301 tataagttat attatcataa taccacaaaa tcacaaattt tcattcaatt atcattttca
     3361 attgcatttt ataaatcatt ttgtatgtaa tttggtatgt aagttaaaat gaaaaaagta
     3421 gtccattttg aagtacattt tgtcattttg tactccaaaa ctgaactact tacttgcata
     3481 tatacgcctt tatagtggag tactacattt acgagattcg gttagagtat cagaaaaaga
     3541 aaaaataaaa gttacagaaa tgtatacttc aataaatggt gaattatgta agattgatag
     3601 agcttattct ggagaaattg ttattttgca aaatgagttt ttgaagttaa atagtgttct
     3661 tggagataca aaactattgc cacagagaaa aaagattgaa aatccgcacc ctctactaca
     3721 aacaactgtt gaaccgagta aacctgaaca gagagaaatg ttgcttgatg cccttttgga
     3781 aatctcagat agtgatccgc ttctacgata ttacgtggat tctacgacac atgaaattat
     3841 actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc aagaaaagta
     3901 tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac cgttaaaaaa
     3961 tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt ccattggttt
     4021 atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg tttctcttgg
     4081 atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg gttgcgaaca
     4141 aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtacg gtttatacta
     4201 tagccctgtt agtactccag cagattttcg gatgcttact cctattgtac tggagcaagc
     4261 ctttagaaaa gctggaacag aattgttaga gccatatctt agttttaaag tttatgcacc
     4321 acaggaatat ctttcacggg catataacga tgctcccaaa tattgtgcaa atatcgtaaa
     4381 tactcaactg aaaaataatg aggtcattat tattggagaa attcctgctc gatgtattca
     4441 agattatcgc aatgatttaa ctttttttac aaatgggctt agtgtttgtt tagcagagct
     4501 aaaaggatat caggttacca ctggcgaacc tgtttgccag acccgtcgtc taaatagtcg
     4561 gatagataaa gtaagatata tgttcaataa aataacttag tgcgttttat gttgttatat
     4621 aaatatggtt tcttattaaa taagatgaaa tattctttaa tatagatttg aattaaagtg
     4681 gaaaggagga gattgttatt ataaactaca agtggatatt gtgtcctatt tgtggaaata
     4741 aaacaagact acgaatacga gtggatacta tacttaaaaa tttcccttta tactgcccca
     4801 aatgtaagaa cgaaacttta attaatgttc aaaaaatgaa tataataaca atcaaagagc
     4861 cagacgccaa gacgcagagc cgataatttg agaaatgaaa ctctcatctt atcggctctt
     4921 tttgtttatc tgaattttac tgactagcct tcaatatttc ttgcttcatt gatacctttt
     4981 gctagtgctt ccattaagga tagttctttg tctgtaaagc tatccatgta tttttctacc
     5041 tgtaaccttc gagtgctttt aatcaaatta tctgtaggta agaaaaattc atcaatagat
     5101 acatggagta atgatacaag atcatacagc acttgtgtac ttgggtgttg tcctttgttt
     5161 tctatatttg ttaaataacg tgggtcaatt tcaatcattg ttcccacttg ttcacgagtg
     5221 agaccccgtt tcattcgagc ctctttaatc gctaaaccaa cggctctaaa atcgtattta
     5281 tcttcttttt tacgcatact aaatcacctc tatacatttt actgttccta tataaccata
     5341 aacaggtata gaaaaacatg ttatatagtt tatgagttcc tatagaacac tgtggaattt
     5401 attaatgcga tgaatccaat ttcaaataga aaggtcgctg attttattta cctatacaac
     5461 tttataaaat ttagtcatac tcctagaaag tagataaaat tggcgactta atggagggaa
     5521 aagtcgccat gagaaagata atctgtcatc tagttctctt aattaagttc ttatcaaaaa
     5581 aacttatcca cctgtactgg atagatatga ccatcaatgt ggttttgcag attacataga
     5641 tagaaaaatc aaagcatatg aacagagaaa acaatctgtt tatgtgcttt tttggtcact
     5701 taataaatat cttttaggat ttctcttcat ttatgcaaca aatcccccgt tcgtgtcggg
     5761 gtttagtatg aagggagata aagaagcaca tgccactccc tttcacgaca gaaagggggt
     5821 gaaaagcatg aaaccatctt cttttcaaga aaccatagaa aatcagtttg actatatctg
     5881 caaacaagtt attgaagatg aacgaaaaga ttacttcaaa catctagaca gactatcagc
     5941 aaaagaagtc tcgttttctg atatagataa ctaccttgtc aactcttttt ctactgttga
     6001 tacgtacgtc actgatttcc aactatttaa attatttgga ttaagaatca atgttgaaag
     6061 tgatttatta agtgaagccc tgcgtcatct ttcagagaaa aaacggaaca ttattttact
     6121 tcattacttt atggatatga gtgatgtgga gattgcggag ttactaaatt tgaatcgttc
     6181 gactgtttat cgtcatcgaa taagtggact agccatgatt aaagaattca tgaaggagtg
     6241 taaagaatga aaacagctta tccaagggtt cccttccccc tcattgtaaa ggcgacagat
     6301 ggcgacgtgg aagcaattaa tcagattgtt aaacattatc gagggtatac ctcaaaacgt
     6361 tcattacgtc gcatgacaga cgaatatgga aattctcata tggttattga tgaaacccta
     6421 cgtggcagaa tggaaacgaa actcatctct aagattttat cgttcgaaat taaatagtcc
     6481 cattttttcg tggaagcgtg tcgaatcgta tccacgcttc ccgatttggg aggtttgtta
     6541 ttccatcaaa gcatattgtg tgttcagtag gttttgatag gctaacaagc caaacgttct
     6601 ttgacaacca aataaaggca atcgaatacg tttcggtaag aaaagagctg atgaactaac
     6661 acgccatgac ctacaaaatt agtgagcgat tcatgttagt aattcgaaag taatctttag
     6721 caggattgct gccatgactt tcttatcgtg ataatgatac tcccgtatag ccatagtccg
     6781 agcgttagaa gcgtcacagg caatgggtac ggctacatga gaaccatgca gaagtgaaat
     6841 tcctgtgagc tttgctagag ctgttcgatt gctaagatat tcttctcttg tcagtgtatt
     6901 attataaaac tatttgatat aattataggt gaatattgga gttttcctag cctataattg
     6961 ggaaaggagt aataatgata acggtcacaa aaaaggattt agttgagcta ggatatggaa
     7021 cttctttttc agcagacatc ataaaaaaag caaaagaatt gatgattaca aaagggcata
     7081 tctactatca atctcgaaag ctagatagag tccctcgtga agctgttgaa gaaattctag
     7141 gaattcggtt ttcaaatgaa aacaggtctg agtagttttt gcacagtaag gagtaaaaac
     7201 tatggttaaa gacccaataa aaaaagcaaa gaatggaacc tattattttc gtgcaaatct
     7261 aggttatgat tctaaaggga aaaaaattca aaaatatcgt agtggattca ctactaaaaa
     7321 agaagcaaga gaagaatatt cgaaactgct attgatgaaa ccagaagaat taagtgagaa
     7381 taaggataaa atgacgttta aacattatat ctttgaaatt ttccttccat ggtataagac
     7441 acaggtaaag ctcagaacat atgaaaatcg cttaccaact attaagaagc atttctctta
     7501 ttttgataaa atggctgttt ccgatattga gcctattgat gtgcaaaatt ggcaactaaa
     7561 attatcaaag aagtgtaagt cttcttatgt aagagctgtt caaggactat tttcagttgc
     7621 tatggatcga gcaattgtcc ttgggataac caccacaaat ccatctaaga ttattggcaa
     7681 tgtgaaaaag caaaagtcta aaattgaatt ttggacaaaa gaagaatttg aaaaagtaat
     7741 ctctctaatt tacaaagaag attactatca acacttttta tttatctctc tgtggttttt
     7801 atttatgact ggaatgcgaa ttggtgaagc tactgccatt caatgggaag atattgattt
     7861 tgactcagga gtactaacca tcaacaaaac actctattac aagaatcaat cgaattatag
     7921 atttgttgaa ccaaaaacaa aggcgagcgt tcgtcatatt gtattagata aatgcacatt
     7981 aacttattta tccgaatgga aggaagtaca acaaaatcta attcagacag actttgtaat
     8041 gagctataac ggtattccta cccaaaaaca tacactatca tatgctattg aacgctatgc
     8101 taagctggct ggtatccatc gaatcagaat ccatgggtta cggcattctc atgcttcctt
     8161 gttaatcagt atgggagaaa atccacttat cattaaagat cgtctaggac acgaagatat
     8221 tgagacgact ttaggaacgt atggacatct ttatcctaac agtaatttcg aggtagctaa
     8281 caaattaaat ggagccattt cctttaaaga agcaaaacaa aattatgata cttcaccaaa
     8341 gaatcaattt actgtagact acttgcgaaa caaagaaatg aaaaaagtgc aataaaagtg
     8401 cagtaaaaag ataagaagag ccagaaaccc ttggtataag aggatttcta gctctcctac
     8461 aactattccc actccactgt tgctggtggt ttagaagtga tgtcatacac gatacggttg
     8521 acgtgggcta cttcgtttac gatacgtaca gagatttttt gcaagacttc ccatgggata
     8581 cgggcaaagt cagcggtcat tccgtcgatt gaagttacag cacggatacc gactgtgtag
     8641 tcgtaagtac gtccgtcacc cataacaccg acagagcgga tccctggaag aacagtgaaa
     8701 tattgccaga tatcacggtc aagtccagca gctgcgattt cttcacgcaa gatagcatca
     8761 gattcgcgta cgatttctag cttgtcttca gtgatttcgc caagaacacg aataccaaga
     8821 ccaggaccag ggaatggctg acgccaaacg attgaatcag gcatgcctaa ctgagtccct
     8881 aattcacgga cttcgtcttt gaataatgtg tttaatggtt cgatcaattc aaattgcata
     8941 tcttctggca gtccacctac attatggtga gatttgatcg tctgtgctgt ttctgtacca
     9001 gattcgataa cgtcagtata caacgttcct tgtgctagga aagagacgcc ttcttcacca
     9061 gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat gattttacgt
     9121 ttttgttctg ggtcagatac acctttcaac ttgtctaaga agcgatctct tgcatcgact
     9181 ttgatgatgt tcaagccgaa ttttccgcct agactttcca ttacttgttc cgcttcgcct
     9241 ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat cgctttttgt
     9301 aaaagaaccc caacgacact agaatcaacg ccacctgaaa gaccaagcaa gacttttttg
     9361 tcaccaactt gttcacggat tttttctact tgcatatcga taaagttatc cattgtccag
     9421 ttaccttcac agtgacaaac atcaaaggca aagtggcgca gcaattcgat tccgtattcg
     9481 gaatgacgta cttctgcatg gaattggata ccataaaaat tacgtgttgt gtcttggata
     9541 gaagcaatcg gacaatcttt gcttgttccg actgtttcaa agccttcagg aactttagtt
     9601 accaagtcac catggctcat ccatactgtt tgttgttttg gtgtttcttt aaacaagaca
     9661 gcttgatcgc ttgttacttc taacaaggct ttcccatatt cgcggttttt agcaggctct
     9721 acggaaccgc caagtttgta cgtgatcaat tgcataccat agcaaatccc taatacagga
     9781 atacctaaat tgaagatctc aggatcgatc ccaaatgcat tctcatcata tacactgttt
     9841 ggtccgccag aaagaatgat tccctttgga ttcatcgctt ttacttcttc tgcagtaatg
     9901 cggtggctca ataattcgga aaagactcca agatcacgaa tacgtcttgt gatcaattgg
     9961 ttatactgac taccgtagtc taatacgata atcttttcaa cgttcgtcat atcggcaacg
    10021 tttgtcaaat ttattacccc tatcccttta aattttatct caaaatagtc aaactatttt
    10081 aaaatgctaa tattttcgca tgaaaatcga atcgatgata tgattttcag ttttatggag
    10141 aattatatcg gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt tcaaattcac
    10201 atttttccat acatcttttg ccattttaaa ggcttcttca cgatctccga tagcaaaacg
    10261 ataataataa ttctctggat tttggaaagc cgtatccagt aacgcaccaa aacgatccaa
    10321 ataccatttt tcgatcagca cgggatcggc atcgacatag acagaaaaat caaaaaagtc
    10381 gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct caacgatcaa
    10441 gatatcaggc tgctggatca attcgtactt gtctggaatg atatcataca catcatgtga
    10501 gtaaacaggt acttttatct cttcctgacc agattttaca cggttcaaga aatcgatcag
    10561 catctccata tcataacttt caggaaatcc tttacggtcc atgattccac gctcttttaa
    10621 tatacgattt ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc ttcgtttgaa
    10681 ggtacgagac aaaatcgtct gcagcagtct ggctgttgta ctttttccaa cggctacact
    10741 tccggcgatc ccgataataa aaggcggtac ttctacgtat ttatgaagaa ataaaccttt
    10801 actcaagaac aaagattcaa attctttcat ataaagataa atcagatgag caagaggtac
    10861 atagatatct cgaacatcct gcatggaaat gcggtcattc aaacttttga tattatctag
    10921 ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat tctcgccatt cttctcttgc
    10981 cacacggtag tagttcattg gatcattcat attagcctcc tgcgttctcc catcaatgga
    11041 actattttaa cacaaaataa acgcaaaccc caccacttaa gagagattgc cgatcttttt
    11101 gtataatctt tataatagaa agaaacggag tcttattctt gacttgcgca cggatacata
    11161 aaaggataga ctgttgtcat gaaagaaatg agaaggatgg aagacatgac aaatcattac
    11221 tattctgaac gcccagatac ggcgcacgat tttgaacaat gggcttttga gttaaaaggc
    11281 agaacctttc aatttgtaac agattctggt gtattttcac gtgaaacagt ggattatggt
    11341 tcacgtgtcc tgattgatgc tttcgattgg gaagagttac ctgatgaagg aaagatctta
    11401 gatgtgggat gcgggtatgg accgattggg ttagctttgg catttgcgac acagcggttt
    11461 gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa cgcaaagcgt
    11521 aaccaaatcg aacatgttga tatccatcaa tcgaatatct atgaagcagt tcatgaagaa
    11581 acctatgcag ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt tgtacatgaa
    11641 attttgacag gcgcatatcc tcggttgaag aagggcggta cactgacaat tgtgatccag
    11701 aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg taatgcagaa
    11761 atcgtcacaa aagataaagg atattatatc atcaaaagtg tgaaagaata actagctgaa
    11821 aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg tatcagttct
    11881 ttttttgttg agaagttttt aaaaacaaag aactattttt tcttcaaaaa gaaatagtag
    11941 gaacagagtc gttgtttttt acgataaaat aaataagata cagaacttgt atagaaggct
    12001 gccaagtcgg tagataaagg aggtttttat caaaaaacat actttccttt tttctctatt
    12061 cttttcttgg gggttttggt acactgatag ggaatttaga tagaagggag ccaataggat
    12121 gaagctgacc atcagagata tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca
    12181 gattttgaat aacaaaggaa gcagatttag tgataagaca agaaaaaaag tgttgtcgat
    12241 cgtgaatgaa catcattata agcccgatta tttcgcttca aatgtgatca atcgacattc
    12301 aaagaccatc ggaatgatcg tccctgatgt cactgatttc ttcttctcca aactgatcga
    12361 aggtgtagag agctatttga atcctttggg ctatgtgatc atgttatgca attctcgtca
    12421 tagccaagaa aatgagatca agtatttaca agagctttcc caccgctcag tggacggaat
    12481 cttgctggcc acaccaaata ttttacctga gaaatatgca ttagacagcg gtttttatca
    12541 aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt cgggataacg gacggcttat
    12601 cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg tttattgaaa atggccatac
    12661 gaagatcggc atgctgaagg aaaccactgg atactatcaa ttagaagaac gtttcaacgg
    12721 atatcgtcat gcgctgaaag atcatggcct gccttttaat gggaaatttg ttgaacatgg
    12781 agagttgact gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac ataaagatat
    12841 cacggctatt ttttgcggga atgatgccat ggcgatcgga tgctatcagg cgatcgatga
    12901 attgggcaaa aaaatcccag aagatatctc agtcatagga ttcgatggat taaagctgtc
    12961 agaatacatg attccgcaat tgacgacagt gcagcagcca agttttgata ttggatttta
    13021 tgctgcacgt ttcctgatcg atacgatcga atttcctcaa agaaaagttc cgaacaaagt
    13081 gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg
    13141 cggtatattg cttatgtaag catttacaaa ggctagttag tgcttttttt atttttagta
    13201 aaccagttta ctaaagtggt ttacctttcc taaaaataat gtataatacc gaatgaagac
    13261 aaggaggatc aggcaatgcg aatggtggat ctgattgaaa aaaagaggga tggaaacgag
    13321 ctgtcgaaag aggaaatcga gtacatcgtc acgaactata cgaatggaaa gattccagat
    13381 tatcaagtca gtgcattatt gatggcaatt ttttatcaag acatgacaaa tgaagaaatc
    13441 acgaacttaa cattggcgat tgctaattct ggtgatgtca tcgatttgag ttctcttgaa
    13501 ggaataaagg tagataagca ttcgacaggt ggcgttggtg acacgaccac attgatttta
    13561 gcccccctag tagctagtgt cggagtgaca gtggctaaaa tgtctggacg cggtctaggt
    13621 tacacaggtg gtacactaga caagctagaa gcaattcccg gatttcagat cgagctttcg
    13681 gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg tcaatcagga
    13741 aacttggcac cggcggacaa aaaattgtat gccttgcgtg acgtcacagc gaccgttgat
    13801 tctcttccgc tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca
    13861 atcgttttgg atgtgaccac tggagacggt gctttcatga agaacatcga agatgcaaga
    13921 cgtctggcaa agaccatgac gagcattggg aaactggcta accgagaaac agtggcggtt
    13981 atctcagata tgtcagaacc tcttggcgaa gctatcggaa atagtctaga agttgtcgag
    14041 gcaatcgaaa cattacaagg aaatggtcca gaagatctag tagaaatgtg ctacgcctta
    14101 ggaagccaaa tggttgttct tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg
    14161 cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat
    14221 caagggggag acccagcgat cgtggaacat ccagaacgaa ttttgactgc acgctacaca
    14281 atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga
    14341 attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag aagaagatat tgatcacgct
    14401 gttggattga aactgcataa aaaaattggt gacactgtga caaaaggtga gtcactattg
    14461 acgatctata gtaacgacga agaaatcact tctgtcattg aattattata taaaaatatt
    14521 gaaattggtg aatcggcaat gaagccaacg ttgattcatg acatcatcac agaataaagg
    14581 aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag aaatggaact aaaccgaatg
    14641 atcgaccata ctattttgaa agcagacgca tctaaagaag ctgtgatgca aattatcgaa
    14701 gaagcaaaaa aataccattt ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg
    14761 aaagaacaat tacaaggtga gccagtagcc gtatgtacag taatcgggtt tcctctagga
    14821 gcaaacacac ccgagacaaa agcatttgaa acaacagatg ccatcaataa cggtgcagat
    14881 gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca acaagtccaa
    14941 aaagatatcg aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa agttattatt
    15001 gaaacatcgt tattagatag agaagaaatc atccgagctt gcgaaatagc aaaagctgca
    15061 ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa ggtagaggat
    15121 gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc
    15181 cataacgaag aagaagcaat ggcaatgatc gaagctggtg ctacaagaat cggaacaagc
    15241 gcaggagtag caatcgtatc tggttcaact ggtgaaggct attaattttt tcaaaggaga
    15301 agctcatggc agaactcaaa caagaatgga tcaacacagc tattgaagcg ttagataaag
    15361 cctatgttcc ttattcccat tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga
    15421 tttatcaagg catcaacatt gagaatgcat catttggatt aacgaattgt gccgagcgca
    15481 cagctttttt caaagcagta tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg
    15541 ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg gcagaattct
    15601 gttcccctga tatgccggtt acgctagtag gagacaatgg tgtgacgaag gcaacgaccg
    15661 ttcgtgcact gctgccttat gcatttactg aaaaagatct ataactcatg ttcattcagt
    15721 cagctgactg ttgaaaaata aaacaattat tttaggaggc tttacagaaa tgaaaaaagc
    15781 aaaattattt ggtttaggtg ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg
    15841 caacaacggc tctactgatt catccggtgg caaaagctca tcagacacaa caactgcagc
    15901 attgatcaca gacacaggtg gtgtagacga ccgttcattc aaccagtcag cttgggaagg
    15961 actagaaaaa tgggggaaag atcaaggact ttcacgagga aatgatggct tccaatattt
    16021 ccaatcttca aatgaatcag attacatccc aaatattgac caagcattga atgctggatt
    16081 caaaacgatt tttggtattg gttataaact aaaaccagcg atccaagaac aagcaacaaa
    16141 caatacaggt acgaactttg ttattatcga tgatgtcatc gatggattag ataacgttgt
    16201 atctgcaact ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag cagcttatac
    16261 gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt
    16321 tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa
    16381 agtattgaat caatatgctg gtgacttctc tgctccagat aaaggacgtt caattgctca
    16441 aggtatgtat gcacaaaacg cagatatcat cttccatgct tcaggcggta caggtaacgg
    16501 tgtattccaa gaagcaaaat cattgaacga atcaggcgac aaaaaagttt gggttatcgg
    16561 tgttgaccgc gaccaatcag acgaaggcga atacacatta aacggcgaaa agaaaaactt
    16621 cacattgaca tctacactga aagcagttgg aacagtagta gaagatttgg cacaaaaatc
    16681 agcggacggt aaattccctg gcggcgaaca cactgtttat ggattgaaag aagacggtgt
    16741 tggtctaaca gaaggacaac tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga
    16801 aaaaatcatt tcaggtgacg taaaagttcc agaaactcct gaagaaaact aaaaccattt
    16861 ctatagcctt gttagacaca tgtttgacaa ggctatggtt tgattagaac aagaattgtt
    16921 catttatcca agctgccttg tgacagtttg gataagggaa caactccctg aagtaatgtt
    16981 tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt caatcgttgc cctagataga
    17041 aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa atgcagaata
    17101 ttaccaaaga atttggaaca ttcaaggcaa atgacaacat caatttacaa gtaaaggcag
    17161 gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa atctacattg atgaacgtgc
    17221 tttccggatt attagagccg acatcaggga aaattttgat gcgtgggaaa gaagtaaaga
    17281 tcacaagtcc gaccaaagcc aaccaattag ggattgggat ggtccatcag cactttatgc
    17341 ttgttgatgc ctttactgta acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag
    17401 ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa cagtatggat
    17461 tgtcagtcaa cccagatgct tacgttcgtg atatttcagt tggaatggaa caacgggtag
    17521 aaattttaaa aacactttac cgaggagcag atgtactgat ttttgatgag ccgacagctg
    17581 tattgacccc tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag
    17641 gcaagtcgat cattttgatt acgcataagt tagatgaaat caaagcagta gctgaccgct
    17701 gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac
    17761 aacaattagc cgatatgatg gttggaagaa cggtttcatt caaaacgatg aaaaaagaag
    17821 cgaagcctca agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat
    17881 tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg
    17941 gaatcgatgg aaacgggcag tcggagttga tccaagcttt gactggtttg cgaaaggcag
    18001 aaagcggaca tatcaagcta aaaggggaag acatcactaa taaaaaaccc cgaaaaatca
    18061 ctgaacatgg tgtaggacac gtgccagaag accgtcataa atacgggtta gtcctagata
    18121 tgacattatc tgaaaatatc gcactacaaa cctatcatca aaaaccttac agtaaaaacg
    18181 gcatgctgaa ttattcagta atgaatgaac atgccagaga attgatcgaa gaatatgatg
    18241 ttcgaacaac gaatgaactt gttcccgcaa aagctttatc aggcggaaat cagcaaaaag
    18301 caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca aatccaactc
    18361 gtggactgga tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa caaagggaca
    18421 aatacaaagc agtgttactg atcagtttcg aattagaaga aattttaaat gtttcggatc
    18481 gtattgctgt tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg
    18541 aaaatgaatt aggtctattg atggcgggct ataccttaga agaagcacga aaagagttag
    18601 aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt attagtgcct
    18661 gtactttcag ttgttttagg attgatctta ggcgcgatat tgatggctgc ttttggtttt
    18721 cagcccatcc aaggttattc ttctatgcta gatgcagccc ttggttcgca aagagggata
    18781 ggtgaaacgt taaggcaagc aacgccgttg atttttacag cattaggttt ttctgtagct
    18841 aactcagctg ggtttttcaa tattgggctt tctggacaag cactttgcgg gtggatcgtc
    18901 agtgtctggg tggcacttgc gtttccagat ctgccaaaag ttgttttgct tccgttatgc
    18961 gtgatcttag gtgccttagc tggtgcgatg gcagcggctg ttccaggttt acttcgagca
    19021 ttttttggaa caagtgaagt gatcgtaaca atcatgatga actatatctt attatacgta
    19081 agcacatttg ctttacatga agtgatgcct gaaagctatc gctcaagttt ggactcttcc
    19141 aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc ttttggcggc
    19201 tctcgcgtaa actcaggatt atttttagca atcatcgcgc ttattttcgt ttggctgctg
    19261 atgaagaaaa cgactttagg atttgaaatc cgttctgttg gattgaatcc ttttgcttct
    19321 gagtatgcag gaatgagtag taaacgtacg atcgttttgt cgatggtcat ttctggcgct
    19381 ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa
    19441 acgacttcgc tatctattgg atttgacgga atggctgttt ccttgcttgg ttctggatca
    19501 gcaatcggta ttcttttatc agcgatcttg ttcagtattt tgaagatcgg tggacttggc
    19561 atgcagacag gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc tttgattatc
    19621 ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa
    19681 aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg
    19741 ggcgaactat aatgtcgact atcgaattaa ttgccttgat ccttacttct acactggttt
    19801 attcaacacc gctaattcta acgtcactag gcggaacttt ttctgaacga agcgggatcg
    19861 taaacgtagg gctagaggga atcatggtca tgggtgcttt tagttccatc gttttcaact
    19921 tgacgatgag tccatcactt ggttctatga caccatggat cgctatttta gtgggtggcg
    19981 tcgtaggtat cctattttct ctgattcacg cagtagcaac agtcagtttg cgtgctgacc
    20041 atattatcag cgggacagta attaatctga tggcacctgc tttaggtgtt ttcttgatca
    20101 aagcatggta tggtaaaggt caaactgata acatcacgca gaatctgggg tatttttctt
    20161 tcccaggatt agcagatatt ccggtcattg gaccaatttt ctttaaaaat acattcttac
    20221 ctgcttattg tgctattttg attgctgtcc tagcttgggt gatcatcttc aagacgaaat
    20281 tcggcttgcg cctccgttca gttggtgaaa atcctcaagc tgccgacaca ttaggtatca
    20341 atgtatatgc tatgcgttat tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg
    20401 gtgctgtttt tgctcagtcg atttccggta acttttcagc tggtacgatc gttggacaag
    20461 ggttcatctc catggctgct atgatctttg gtaagtggaa tcctttaggg gcaatgggtg
    20521 cctcattgtt ctttggtttt gctcaaagct tgagtatcat tggcgcacaa ttaccagtta
    20581 tttcttcgat tccgccagta ttgctgcaaa ttgctccata tttgctgaca atcattgtgc
    20641 tggttgtttt cttagggaaa gcttctggtc caaaagcaaa cgggaagaac tatatcaaat
    20701 caaaataatc ttttagcgaa taaaaagggt gggttacggg aagtgttttt cgtctcgccc
    20761 tttttgtttt aaaggcaata aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt
    20821 acatctgatc gtcatggatt cagtaggaat tggagaagca ccagatgccg ataagttcgg
    20881 tgatgttggt tctgatacat taggacacat tgcgaaagaa gcaggattga acgttccaca
    20941 tttagagcag cttggtttag gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga
    21001 tcatcaaggc tatgcgacga aattagaaga agtttctgta gggaaagata cgatgacagg
    21061 ccactgggaa atcatgggat tgaatatcaa aactccattt cgtgtgtttc ctaatggatt
    21121 ccctgatgat ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa
    21181 accttatagc ggaacagctg ttattgacga ttacggtgaa caccaaatga aaacaggcga
    21241 tttaattgta tatacttctg ccgatcctgt ccttcagatc gcggcacatg aagaaatcat
    21301 tcctttagaa gaattatatc ggatttgcca attcgttcgt gatataacaa aagatgatcc
    21361 gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg
    21421 aacaagcaat cgccatgatt atgcgttgaa tccattcgga cacacagtcc ttgattcttt
    21481 gaaagaagca ggaaaggacg tgattgctgt tggaaagatc aacgatattt tcaacggaca
    21541 aggaatcact gaatctgtcc gcacgaaaag caatatggat ggcgttgacc agctgctgaa
    21601 agttatgaaa aacgattttt caggaatcag ctttaccaat ttagtagatt ttgatgcatt
    21661 atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat ttgacttacg
    21721 aattccagaa ttgcttgaag cgatggaaga agacgacttg ctaatgatta cagccgatca
    21781 cggaaacgat ccgacgttta ctggtaccga tcatacgcga gaatatgtac cgttattggt
    21841 ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa ggatattatt cagatatttc
    21901 agcaacgatt gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa
    21961 attattaaaa taatcttttt aaataggaga aagcaaatga ccaagttaag cgagatgtta
    22021 caagaaacga cccagtttat ccttgataaa ggagtaaaag aaattgattt tggtttgatt
    22081 ctaggttcag gattaggtga attagcagat gaaatcgaag atgctgtaat gatttcttat
    22141 gatgagatcc cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa gctagtttat
    22201 gggtctctgg caggcaagaa agtgttagcc atgcaaggga gattccacta ctatgaaggt
    22261 cattccatgc aaacagtgac ctatccagta cgtgtgatgg ccggactagg ggcccattca
    22321 gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg agatttgatg
    22381 ctgattaccg atcatatcaa ttttactggt gaaaatcctt tgatcggtcc gaatgaagat
    22441 gcaatgggcc ctagatttcc agatatgagt caagcttata cgcctgacta tcaagcaaca
    22501 gcaaaagcag cagcagaaaa actaggtttg gatcttaaag aaggcgtata tatgggctat
    22561 tctggaccaa cttatgaaac gcctgcggag atccgaatgt ctcggacaat aggagccgat
    22621 gcagtaggga tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg attaaaagtg
    22681 ttaggaatct cttgtattac caacttagca gcaggtatgc aagcgaatct gagccatgaa
    22741 gaagttgtag aaacaactca gagagtcaaa caatctttta aagcattggt taaagaaaca
    22801 ttgacattat tgtaatagga ggaacaataa aatgagtgtg catattgaag cgaaagaagg
    22861 cgagatcgcc gataagattt tacttccggg agatcctttg cgagctaaat atattgcgga
    22921 gacttttttg gaagatccag tttgttacaa tcaggtacgc ggtatgttag gctttaccgg
    22981 caaatataaa ggagaacgta tttctgttca aggaacagga atgggaatgc catcagccac
    23041 catctatgcc catgaattga tccagtcgta tggtgtaaag aaattgattc gagtaggtac
    23101 atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta gtactagcac aaggtgcagc
    23161 gaccagttct tcgatgatcg aaaaaaattt ccaagccttt cattttcctc caatcagtga
    23221 tttcaatcta ttactgaaag catatgagat agctaaagaa aagggctata cggttcatgt
    23281 aggaaatgtt ctttcggaag attctttcta caaagatgat ttgacagaga cgttccaatt
    23341 agctgaactt ggcgttttag gagtggaaat ggaagcagcg gctctttatt acttaggcgc
    23401 gaaataccat gtgcagacat taagtttgat gaccgttagc gaccatttga tcaccggtga
    23461 agaaacaaca gctgcagaac gtcaatctac gttcaatgaa atgatcgaag taggattgga
    23521 aacagcgata gcagaataag aaggaaatga aaaagctgcc ctgcattaag taataagaac
    23581 atccaagcaa aaatagctcc tcatgttttt gacttggctg gacttattat tgcaaatgta
    23641 agcttttgaa caccgtttac caagaggttg tgaatctgcc gtttaactct gagtcacaag
    23701 gaacaatttc aaaaa
//
LOCUS       DAFEHS010000210        18008 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.guided.056, whole genome shotgun sequence.
ACCESSION   DAFEHS010000210
VERSION     DAFEHS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.guided.056"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            <1..324
                     /locus_tag="LP327_002873"
     CDS             <1..324
                     /locus_tag="LP327_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL1726906.1"
                     /translation="SRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRP
                     ENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLS
                     SVENK"
     gene            complement(474..1415)
                     /locus_tag="LP327_002874"
     CDS             complement(474..1415)
                     /locus_tag="LP327_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL1726907.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1541..2662
                     /locus_tag="LP327_002875"
     CDS             1541..2662
                     /locus_tag="LP327_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL1726908.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2920..4422
                     /gene="eat(A)"
                     /locus_tag="LP327_002876"
     CDS             2920..4422
                     /gene="eat(A)"
                     /locus_tag="LP327_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL1726909.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4450..5016
                     /locus_tag="LP327_002877"
     CDS             4450..5016
                     /locus_tag="LP327_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL1726910.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5227..6287
                     /locus_tag="LP327_002878"
                     /pseudo
     CDS             5227..6287
                     /locus_tag="LP327_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
     gene            6290..7186
                     /gene="murQ"
                     /locus_tag="LP327_002879"
     CDS             6290..7186
                     /gene="murQ"
                     /locus_tag="LP327_002879"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL1726911.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7200..9182
                     /locus_tag="LP327_002880"
     CDS             7200..9182
                     /locus_tag="LP327_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL1726912.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDFAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9247..9693)
                     /gene="nrdI"
                     /locus_tag="LP327_002881"
     CDS             complement(9247..9693)
                     /gene="nrdI"
                     /locus_tag="LP327_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL1726913.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            9904..11328
                     /locus_tag="LP327_002882"
     CDS             9904..11328
                     /locus_tag="LP327_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL1726914.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11509..12282)
                     /locus_tag="LP327_002883"
     CDS             complement(11509..12282)
                     /locus_tag="LP327_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL1726915.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12279..13184)
                     /locus_tag="LP327_002884"
     CDS             complement(12279..13184)
                     /locus_tag="LP327_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL1726916.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13338..13658)
                     /locus_tag="LP327_002885"
     CDS             complement(13338..13658)
                     /locus_tag="LP327_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL1726917.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14132..14818
                     /locus_tag="LP327_002886"
     CDS             14132..14818
                     /locus_tag="LP327_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL1726918.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            14820..15764
                     /locus_tag="LP327_002887"
     CDS             14820..15764
                     /locus_tag="LP327_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL1726919.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            15963..16607
                     /gene="pcp"
                     /locus_tag="LP327_002888"
     CDS             15963..16607
                     /gene="pcp"
                     /locus_tag="LP327_002888"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL1726920.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            16686..17963
                     /locus_tag="LP327_002889"
     CDS             16686..17963
                     /locus_tag="LP327_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726921.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         5752 a         3193 c         3846 g         5217 t
ORIGIN      
        1 tcgcgcggga ttctggaact gttgcgggag ggtcagcaag gagtgacttt gcggacattt
       61 gaatccatgt attttcaaaa gaaactagtg acggataatc aagcgattgc tcattatcat
      121 ttttatcgtc ctgaaaatat ttttttgctt cgagaacgtg atttagatga attaccggaa
      181 tttctgcata caccatttaa gccaatccta tcagagctat tggaatttta tgatgcagaa
      241 aattgggcaa aacgatttat cactaacgat tccactattt atgagcaata cgaatatgac
      301 ctctcctctg ttgaaaacaa ataaaaaacc tacattttga agagattatg tactgaaaca
      361 aacaagcaca attcttatgg catcatgaga gttgtgtttt ctttttgatc ttttattggg
      421 cttaaaaaaa gagaaccagc tgctgattca gctgattctc atcagagagc cttttattct
      481 gcgccaagga ttccttcttt tgcatggact aattgggtca aaaaggcaca atatggtgta
      541 gcaatatcat atttttgtcc ttttcgccaa atggcaccat tgatgtagtc gatttcagtc
      601 aaacgatggt ttttgatcag gtcttggtac atagatgggt aatgcaaccc gatgttgtca
      661 ggatcataag tagaagcaat atgttggtac acttcttctt gatctagtat gattccttct
      721 tttgctgcga tagaagcaaa ttcagatacg attgttcgaa ccatgctctc tgccgctttt
      781 tgcgcgccta gttcagcaat attacaatcc aaaatcgtgc ataaaccatt caatgttcca
      841 tttacacaag cttttcgcca aatggaataa cgaacgttat ggctatagat cggatttaaa
      901 tttgcttctt gaaagacttc tacaactttt tttgtaaatg cttcgccttc tggttccaag
      961 ttctctagtt cgatttctcc atcacctaac aatgtgactt ttccgggacc agctagacca
     1021 gctgtccaca tcgtgatccc aaataagata tttttctttg gcacatattt ttccagtaca
     1081 tcttcatgac ctaatccatt tagtaaacac aaaacataag tattgtcagt aataatggat
     1141 tggatcgaac aaaacatatc atctaattga ttggcttttg ttaatgctac gatcaagtcc
     1201 acgtgttcgt tgctttcgat gatttcttct ggtgaataga ttggcagttt tgcgaccact
     1261 tctttaccgt taaagtcagc aatcaaccca tttgtacgaa tggcttcaat gtgagcaggc
     1321 caacgatcga tcaagaggac ctcatttccg ctttgatgta gcatcaatcc gattcggctg
     1381 cccattgctc cggccccagc tattgcgatt ttcatgtttt ttctctcctt tattcaatga
     1441 gttccctttt agtatataga aaaatggaag aaaatagcat tgttttgttt gaaaaaaatg
     1501 aaaaaaagtg tcttacttaa gaagaaagtg agggatacag atggatattt tggcggcaat
     1561 tgattcattt aaaggaagtg cttcttcatt tgaactaaat caggcagttc taaatggctg
     1621 ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca gatggcggag aaaatactgc
     1681 agaagcaatc tattacgcgc tcaggggaac atggcgtacc attgatggga tggatctctt
     1741 attccgtcag cgaaaaatca gctatttatt gacaacttgg caaggaaaaa acaccgcagt
     1801 catcgaatct gcacaagcat tggggttaga tctacttgat aaaccaacgg atcaaactat
     1861 ccgacttgct tccagttatg ccttaggaga acttattaaa gatgcactgc ttcaagaagt
     1921 agaacaaatt attgtcactt taggaggaag cgggtgtagc gatggtggtc taggattact
     1981 ccaatccttg ggtgcttctt tagaagggta cacagaagga aatcctttat tatctactaa
     2041 aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa gtaaaattga ttatcgctgc
     2101 agatgttacc aatccatata caggtcaaca aggagctgct tggcaatttg gaagacaaaa
     2161 aggaggaata gatacgacgc tagctttctt agataaacaa gcaggaaaga ttgctcagca
     2221 aataaaagat ttctgggagg tggatttaga tgatattcca ggaagtggag cagcaggcgg
     2281 tattggtgga gccctttttt tgttgggagc gaagatggtt tctggtttcg atttagtcag
     2341 ccaaatcact ggattggaaa aaaagatcga acaagcagat gtcatcatta caggagaagg
     2401 gcggatcgat cagcaaacga tccacggaaa agttccatac ggtgtagcag tgcttgcaaa
     2461 aaaatatcga aaaccaataa tcgctatttg cggaagtaga gaccaagagc tagaaggact
     2521 ttctgatttt ctcccaattg tcttaagtat tcatttgggt ccaataggtt tagaagaagc
     2581 aatggaacac aggcaaacat tagaaaaaac aaaaatctta ggacagacac ttagcagatt
     2641 tttcaccatc ctcccgaagt agttgacaaa atcattttct ttatgctatc gtaatttcaa
     2701 atataggaca aagtgaggga tccgtgcatt gaaaaattaa gtcgattgat gaaagctgca
     2761 ttcagactgt accttgtgga gacaaggaag cagtgtgcag attttcggga ttggcttggt
     2821 tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gcatcctctt tagccagttc
     2881 tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa
     2941 aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc aaatctaaat
     3001 tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa gacaacttta
     3061 ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca gcaagaattt
     3121 gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt gttaaatgat
     3181 attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca aacagatcct
     3241 gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa agtcctactg
     3301 gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac gaatcatttg
     3361 gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca aggcttcatc
     3421 gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt agcaatcgaa
     3481 aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca gaaaaaactt
     3541 cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag
     3601 aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa aacaaagaaa
     3661 caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt gggtgctgat
     3721 gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac ccagatcagc
     3781 gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat gaatagccaa
     3841 gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta tgaaaatctg
     3901 ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat ttcaggtcct
     3961 aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa cggcaaggtt
     4021 ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca aaattatgaa
     4081 gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta ccaagcattt
     4141 ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa gatcgagcag
     4201 atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca gccagctgaa
     4261 ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga acaattagaa
     4321 caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca aaccttcctg
     4381 gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta atctcaagaa
     4441 caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat gctttatgta
     4501 acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg ggcagatgtg
     4561 ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt agtagatttg
     4621 cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga tacagcacaa
     4681 gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt agaaatggat
     4741 tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg
     4801 ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg gatcgttcct
     4861 ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg ccataatgcc
     4921 ttgataagag tgatcaatgc ttattttcat ccgatgccta atgaggggtt ctttacattc
     4981 atggtagata atacagaatt ggtttcttat gagtaattat gtttttgtgt tatttaaata
     5041 aaatatttca aggcactgct tttgcttagc agtgcctttt ttaggttatg acaattcttt
     5101 tgataatata aactcaacct ttagaaatga aatttaatta ttttaataaa agtattgaaa
     5161 ttttgtttct ttagatatat actctagtca tagaaatgaa gacggataca gaaaaagagg
     5221 gataatatgt ttggcttttc aatatttatg aacgattctt tgtcagaaga gaagaaacag
     5281 tatatccgcc aaatggcatc caatggcttt gtcggtattt tcacttccat gcatatacca
     5341 gaagatgatg tttcggcata tcaaaagcga ttgatcgaac taggaagcga agcgaaaaag
     5401 aataaactag aattgatggt cgatatttca ggagaggcat tggaacgagc gggtttttca
     5461 ttagaagatt taagcctttg aaagcaattg gtgtaacagg tttaagaatg gattatcata
     5521 tttccaatca gcagattgcc gactggtcac atcaattgaa aatcagtttg aatgctagca
     5581 cgatcacgcc taaagatatc gatgaattga aagaagcaga agctgatttc tcacagattg
     5641 aagcttggca taattattat ccacgtcccg aaacagggct ggacaaagaa tggtatcaga
     5701 aaaaaaatca atggctcaaa attcaaggtc tccttgtcca aggttttgta ccaggagata
     5761 cagagttgag aggcccacta tatcaaggat tgccaacttt ggaagaacat cgagggatcc
     5821 atcctcttgc aggcgctcta gatctattag caagtaacac ggatattgtt tacatcggag
     5881 atgcaggtct gtcagaaaac gttcaggagc aatttgcttc tttccaaaaa gaacagacag
     5941 tcttgctcca tacggaacct gtagatgaag aattttacga atatatatta gggaagcata
     6001 cgaatcggca agacgatgca agagatgtca tacgaagcgt agatgcacgt tttcgagaga
     6061 ttccgcctat cccagcaaga aatacagcta cgcgtatgaa agggtctatt actttagata
     6121 acgaaaagta tttgcgctat atgggtgaga tccaattaac caaatatgat ttgccagcgg
     6181 atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga gctaccattg atcaaccaaa
     6241 tcaaagctgg tatgaactat caatttataa gaaaagaagg aagataaaaa tgagcaaaat
     6301 caatttagag aacctaacaa cggaacgcag aaaccaagaa acttttggat tagatgagat
     6361 gagtatttct caagccgttc aactaatgaa caaagaagat cagaaagtgg ccgctgcggt
     6421 tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac acgattgagt cctttaaaaa
     6481 aggtggacgt ctgatttata tgggagccgg aacaagtgga agactaggtg tgttagatgc
     6541 tgctgaatgc gtaccgacat tcggtgtaga accggaaatg gtcataggtc taattgctgg
     6601 tggagaagaa gcaatgactg tagctgtcga aggggccgaa gattcttatg agctagggca
     6661 agaagatcta atcaagctga atttgacaaa aaatgatata gttattggta tcgcagctag
     6721 cggtcgtaca ccatatgtga tcggtggatt agattacgca aaatcgatcg gtgctgtcac
     6781 aggaacgatt tcttgcaata aacaagccga aatcagtaaa cacgcggatt tcccaatcga
     6841 agtcgatgca ggtccagaat ttttgacagg ctctactcga ttgaagtcag gaacagcaca
     6901 aaaacttatc ttgaatatga tttctactat cagtatgatt ggtataggta aagtattcaa
     6961 taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat
     7021 catcatgcaa gcgacagaag ctgattacga aacagctgaa aagtactttg aagaagcaga
     7081 tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat agtgataaag aggaagctca
     7141 agcaaaatta gctgaagcaa acggatttgt aaaagggacc ttatgagggg gaaaagacca
     7201 tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca aatttataaa ggggcaggcg
     7261 gcatgggcaa tgtagaaagc attgtccact gcatgactcg tgtgcgtatg aagatcatca
     7321 atgatgatct tgtaaatgtc tctgaattaa aagcgattcc aggagtgctt ggtgtagtag
     7381 aagatgaaca gcttcaagtc atcatcggac ctgggaaagt gaacaaagta gcacagcaaa
     7441 tggttgattt tgcaggcgtg aaattaggag aaaaactttc ttctgtaagt acagctacag
     7501 caaattcatc agcatctgga aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg
     7561 cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt attaaaagac atttctaata
     7621 tctttgtgcc aatgattcct gcatttgtcg gtactgggat cgtggcaggg attgccgcag
     7681 ttttagcaaa cttagtcaca gccggaacac ttgatgcagc aacatggcaa caatatatcg
     7741 atgtgatgaa catcttgaaa aacgggatgt ttagctacct agttatctat acaggtatca
     7801 atgctgcaca agtttttggt gcaacaccaa ctcttggtgg agttatcggg gccgttgtga
     7861 tgctgacagg gatgaatccg gaagcaccac tgaaaaactt atttactggt gaagctcttg
     7921 cagctggaca aggtgggatc ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg
     7981 agaaaaaact gcataaagta gtacctgatg cggtagatat catcgttaca ccaactcttt
     8041 cattgattgc catcggtctg attgaaattt tcttcatcat gcctttagca ggtttcattt
     8101 ctgatggcat ggtcggtggg atcaactggg tacttggcgt tggtggagca tttgccggct
     8161 tcgttctagg cacattattc ttgccgatgg ttatgttcgg actgcatcaa attttgactc
     8221 caatccatgt gcaaatgatt gatgaaactg gtcgtacgct attgcttcca attcttgcga
     8281 tggctggtgc aggacaagtg ggtgcagcat tggcattatg gattcgctgc aaaaaagata
     8341 aagaattaac agaaatgatc aaaggagctt taccagtcgg gatcctagga atcggcgaac
     8401 cattgattta cggagtaaca ttacctctag gacgcccatt tattactgct tgtatcggtg
     8461 gaggtatcgg cggggcagtg atcggaatga tcggaaatgt cggggctatc gctattggac
     8521 cttcaggagc cgctttgatc ccattgatct ctgacggtaa atggttaggc tacatccttg
     8581 gattattggc agcttatgct ggtggcttcg ttgctacatt cttcttcggt attcctaaag
     8641 accgtttaga aaaagaagaa ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa
     8701 aagtagtacc tcaaacttca gctgaaagtg tgactatcct ctcatcagtt gctgacggaa
     8761 cagttgtttc tttagataaa gcaagtgatc cagtttttgc acaaaagatg atgggtgaag
     8821 gctactttgt agaaccagaa aacggacata tctattctcc agttactggg gtagtcagct
     8881 ctgtcttccc aacgaaacac gctattggga tcacaacgcc aaatggttta gaaatcttgc
     8941 ttcatatggg aatcaacaca gttgacttag gcggtacgcc atttgatgtg aaagtaacag
     9001 aagggcagca agtgacacct gatacgcttg tagcagaagc tgacttagaa gcgatcaaag
     9061 cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact
     9121 ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt
     9181 aatatgatag aaacaataga aacgaaagaa gaaactgaaa tctatttttc agcttcttct
     9241 tttttattat ttattgtttt ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt
     9301 tccccgtaat tcataatctg ccaagaaagg aaagccaaat gtttgtgcat attgtttggc
     9361 tgttagacaa tactgattat tgaagttttt atttccgcta ccgactacac caagacaaag
     9421 tttatgattg tcttcatgtt ccagatattc acgcatcgtt tcggtcaaga tttctgtatc
     9481 accgttatct aaaccatttc cgccgtctaa ataagtagga acgaaagtaa aaaatggttc
     9541 tgtttctttc gtaaaatcac tattttcatg gatttctttt aaagtaactt caggattgac
     9601 aggatcttct gcatgcattt tttcagcata ttctgctaga tgtttggcaa aggcacgcgt
     9661 atttcctgaa atcgatatat ataaaatgtt catggatcat cctccaattc tttcctttta
     9721 gtatagcatc ttttttaagt tttttggtgg tgtaatttgg aaagggacta ggcgctttgc
     9781 ttgtataatg agaaaaaagc cggtagaatt atatttctat taaagttttt attaattaag
     9841 taaaaataaa aaaggtttgt tatacttttt gataaaagcg gttttatttt ggaggatgct
     9901 tacatggata cagagcaaca gaaaaaagga attggtttaa ttccacttgc tgccttagta
     9961 attggttcag cgattggagg tggcgtattt ggtattatga cggacatctc cggatcggct
    10021 ggcggcccag ctattttcag ttgggtactc gtaggaacag caatgttgat gctctccttg
    10081 tcgatcaata atatcaataa aaaacgtcca gatttaaaag gcgggatctt tagttacgca
    10141 gaagaaggtt ttggccgttt cgctggtttt atcagcggtt ggggttactg gctcacatgc
    10201 tggttaggca acgtagcttt tgcaacattg ttgatgagcg cattaggata tttcttccca
    10261 gtatttgaag gtgggcaaaa tctggcatcg atcgtgatga gtacagtctt tttatgggga
    10321 tatgcatggc tagtgaatcg gggagtcgaa aatgctagtt tcgtcaatgc tatcgtcaca
    10381 ttctgtaaat tagtgccatt atttttattt attgtagtag ctatcagcac gttttcgatc
    10441 caacaattca ttgataactt ttctagcgac attatattga atgcgaatgg acagccgatc
    10501 ccgttttcca gtcaagtatt gagttgcatc atggtgatgc tttgggtatt tgttggtatt
    10561 gaaggagcct ctgtcgtagg ttcacgagca aaaaaacaat cagatgttgg gaaagcaacg
    10621 attctaggga tattcggctt gattattatt tacgtcttgg tttccatgct tccatatggg
    10681 acaatgacga tgaatcagct gcaaacgatc aaggcgcagc ctgctatggg ctatgtgttt
    10741 gaaatgatgg ttggtaaatg gggagcagtc gtgatcaacg gcggtttgat catttcttta
    10801 gttggtgttt ggctctcatg gacgatctta ccgattgaaa caatgggaaa catggctgaa
    10861 gacggacttt tgcctaaagc atggggaaaa ctcaacaaaa atggggcacc aacttatgct
    10921 atcgtattga cgacgttatg tacgaatgtc tttttacttt cattattatt cactgacagt
    10981 gcttataact ttgcttacac acttggtaca gcagctattt tcttcacgtg gcttttctta
    11041 ggattgtacc aaatgaaact ttcttatcag cggaaagaat gggtccaatt tttagtaggg
    11101 cttttagcaa gtgcctttca agtatgggct atgctagtcg tagcctttaa tgaagtaatg
    11161 cttgttcttg ttcttttctt gccaggcatt tatttttacc tcaaagcacg taaggagcaa
    11221 aacgagcctg tacaacatat ccacttagat aagattttac ttataggtgt gacgatggtc
    11281 ggagtgattg caatggtttt atttgccact gggatgcttc acgtatagaa gtataaaaga
    11341 agtataaaga gaggttgtga gcctgtcgtt tattcaaaaa catactagga aaggagctgt
    11401 gacacaaatg ttgtcacagc tcctttttca gaataaacgg tgtttaagag acagccaatc
    11461 ggcaataagg ctgaacgtct ctttcacagc ctcttttttg atgagtcatc atacattttt
    11521 tcgcttaaat acgagatatc ctaatgctaa gaacaaaacg atgtaagcta aattaccgaa
    11581 tagcatttga ttagtagaaa gatgagtcaa ttgcttcatg atctcttcgt ttccaatttg
    11641 gttttgcaga ttcaacatac tgatcggatt ccatttgatc cactcccatt tttggatgag
    11701 tgcaataagg ataccagata tgattgacga agcaaaataa aagacgattc ctgctgagat
    11761 ggatgcgccg gaactattga taaagcaagc aatcatcaac actaaactta aaatcagcca
    11821 taaaccgata taactaccca aagtataagt gaacaagctt tggatcagtg tttgaccagt
    11881 agaaaccttc tccgtgaagg aaatcgaagg gaaaagaatc agtttcatca acacagtgac
    11941 aataatagct aaaaggtata aataaacaga ataaataact aatgtgatcc atttgcttac
    12001 taatatttct cctcgagaat actttcgata aaggagattt ttcaatgtac catattgtgc
    12061 ttccatcgaa atgatcgtgc ttgcagctgc aatcatgata aagacaatcc aagatgtggc
    12121 actaaaaagc tgagcagtca tttcaactgg atcaatgatc gtcgtgtatt ttttggctag
    12181 aagtgctgtt cctatgagca aaacaactaa tagaacagat aaaacagcag ttgacttttt
    12241 cttgatcaat ttaaataatt cttgttttac aagcaatgtc atttttcttc ttcccccttt
    12301 actgaatcca gaatttccaa tagagaagat tccaaatcat tcgtcagatg ctggacgtct
    12361 gtcacatgga tattttgtgc agacaatagc tggatcacgg ttcctaataa ttcttcgttt
    12421 tcttttaaaa gcttgatttt atcaggatct tccaaagtat atccagcttc tgtcaagatc
    12481 gttcgagcag ctagatcatt tgttgtagag ataacgatga aatcttgcgc tgtttggttc
    12541 aattcagcca ttgtacattg tttgatgatc tttccttgat tgatcaggat cagctcatcg
    12601 accatttttt caagctcgct gagtaagtga ctggagatca agaaagaggt gccctgagta
    12661 gccaagtcgg taatgatttt tcgcacatct cttgttgctt gtggatccaa accgttcatc
    12721 ggctcatcta aaataacgat agacggatgg ttcagtaagg caagagcaat tcccaatttt
    12781 tgtttcattc ccaacgagta ggatttcgct tttttgtgaa tgtacttttc catcttgagc
    12841 tgattcacga tagtgtcgat agcagataca tcgttggttt cagagaataa cttcagatga
    12901 tcatagcctg ttaagaatgg atagataccc gggtattcga tcaatgctcc tacttgttcc
    12961 aaccctttat gactagaggg agaaatctca ttttcaccaa tacagattgt tccatctgag
    13021 taatggatca gtcctaaaat agctttcatg atcgttgttt ttccagcgcc attaggacct
    13081 actaatccaa cgattttgcc aggggataag ctaaaactga catcgctgag ggcttgtttg
    13141 tgaccaaagt tcttggataa ttcttggacc gaaagacttt tcaaaaatat tcactccttt
    13201 tctatagata gtacaagcat acacctgatg agtggagaag tcgctaggtc attggactta
    13261 tttcttattt tttcataaaa aaattgaata ttaaataaaa aggcttattt tttctgatca
    13321 gtacttattc caagtgtcta gtggccgcta gtaatcttga tgccaatgat actgatgagt
    13381 agcatcccca caaagaacca agtggaaaga ttcagcttat catgaaacaa gaccacacca
    13441 attatgaccg aaccaactgc gccaatccct gtccagatag gataagagag actcatcggc
    13501 aaacttttga ttgcctttga caaaaagtaa aaactagcaa tcatgccaat cactgtgtaa
    13561 aaagaataat tgattttaga aaaaccatca ctccatttca tcatcgttga ccagaatact
    13621 tccaaaattc ctgctatgat taattccatc catgccatac ttacattcct ccttaactat
    13681 aaaaaaatac acaaaaagag gaaaatcatc ccttcaaaga cctaatggaa tgatcatcct
    13741 ctttatccta cccggtggca gattgtatat ctttttctgt agtatagcac acctatccag
    13801 atatcggcaa tctcatactt tttagtaaag tgtaccaagc actgacggat atatcgttag
    13861 cggaaaagaa gatgaatgct ttgcaatttc atagaaagaa gttgttattt attaatcatt
    13921 taaaaaaaac agtaactaga ccttatttct atatattatc gggcaataaa ttaaaaaaag
    13981 ctttacctta caataagtaa gataattttt gtgataaaaa aataacgaaa aatatttaaa
    14041 aatagtgtct atttcgtgag aaaacaacta tattttttat gtgttttgct ttataattaa
    14101 aaaaatattg tcattttatg gaggaaaata tatggaaaat tatcttagcc ttttaggcgt
    14161 cttgattgtt attctcgggt ttgcatttaa attagactcg atattgatcg taatggttgc
    14221 tttagttgtt acagctttga ctggcggtct tggcattcaa ggaatgttag aacttttagg
    14281 tacaagtttt gtaaacaatc gcgggatggc gatttttatc atcattatgt tggcaacagg
    14341 aacgcttgaa agaaatggtc tgaaagaatc agcggcttcc ttgatcaagc gattcaagaa
    14401 agtatctgct ggtatgattg tagatattta tgggatcttt cgaatgatct ttgcggcttt
    14461 taacgtgagt tttggtggag ttgctggatt tgttcgtcca atcattttac cgatggcatt
    14521 ggggaccatc gaatcaaaag ggttagaagt ccatcctaag catgaagaag aactaaaagg
    14581 tatggcttca gctatggaaa atgtctgctg gttctttggc caagtactat tcatcggagg
    14641 agcaggggcg ttgctagtcc aatcaacact gaaagatcta ggatatgacg taacattggg
    14701 acagcttgct ttagtggaag ttccagtggc tatcacggct ttagttgtgg caagtgtcta
    14761 cttctatctt aaagaaaaga aattagcgaa gaaatattat ccaggaaagg ggcaataaga
    14821 tggacttttt tacaaatcct gatattttat taggcgataa attactggag atcctttata
    14881 tcgtgatggg actcgtattg atttacactg gagtaagaaa tttaacagat aaaaccaatc
    14941 ctcatcgtta tgggtcagcc tttttctgga cggtcttagg ggtagttatt gcaggaggaa
    15001 gatggcttcc ggcaatcgtg aacggtggct tgatctttgc gatgaccata ccagcaattg
    15061 ctaagaaagt cagcaaagga gaaagccggc ttccatcaaa agaatatatg gaaaaaatgt
    15121 cagacaaact aggaatgaaa atttttattc cagctttgag tatcggtgtc tttgcgatct
    15181 tgtttgctct atttaccaat ttaggtgctt tagtcggcgt gggcgtcggt gtttttgcag
    15241 ccatgctaat cttgatgttc ttttctcgag ataatcgacc aagtacattt ttagatgatg
    15301 cagcagatat gctgggcaca gtagggccgt taagcatgtt gccaatgctt ttagcttctc
    15361 taggtgcggt atttacaagc gctggagttg ggacagtgat ttccaatgca gtaggtacga
    15421 tcgttccaga aggaaatgtg aacatcggca tcatcattta cgctcttggc atggtgatat
    15481 ttacggtaat catggggaat gcatttgcgg ccatcaccgt gatgacggtt gggatcggtg
    15541 caccattcgt atttagtcat ggagcgaatc cagcgttaat aggaatggtt gcattgactt
    15601 gcggtttttg tgggaccctg ctgacaccga tggctgctaa cttcaatatc gtgcctgtag
    15661 cgatgctgga gatgaaagac aaatacggag tgatcaaaaa ccagttgttt attgcgttat
    15721 ttatgctagt tttccaaatt gcttatatga ttctatttaa ataatagaag agaggggttg
    15781 tgacagaata attgtcgcaa cctcttctaa cattgtagtg gaacactatt tttttataag
    15841 aatatattaa gtttgatctg ttatgttatt taaactattg tttcctttat ttacgctgtt
    15901 caaggaaaat ggtatgataa aagataacca tatgttaaat caatcggacg gaggaacaac
    15961 agatgaaagt actagtaaca ggttttgatc catttggtgg ggacaaagtg aatccagcct
    16021 atgaagcagt aaaaaaaatg ccagatgaaa tcagcggtgc tgaaatcatc aaagtggaag
    16081 taccgaccgt attcgaaaaa agcagtcaag tagtcaaaga agccatccaa cagcaccaac
    16141 cagatgtcgt gatctgtgtc ggacaagcag gtgggcggtc agcagtctcg ttcgaacgtg
    16201 tcgcaatcaa tctagcagaa gcaagaatcc ctgataatga aggaaatcag ccattcgata
    16261 cggctttaga agaaaacggt ccagccgctt actttacctc attgccaatc aaagcaatga
    16321 caaaaaatgt acaagatcat ggtctaccag cgtacatctc ttatacagca ggaacatttg
    16381 tctgcaatga tatcatgtat cgtttgcttc atttgatcga aacacaattc ccggcaattc
    16441 gaggtgggtt catccacgtg ccattctcac ccgatcaagt gatcgatcgt ccagtgggta
    16501 cgccttctat gtctttagaa gacattgctt cttctttgac ttatgcagtt gaagcagcag
    16561 tagaaaataa agaagatatt tccggaaatg ccggaacaac tcactaattt agcaagaaac
    16621 actggctggt tgctaacgtt atgttagtag ccagcttttt gtaattagaa aggaaagaaa
    16681 cagccgtgga agaatggcaa tccgtctttg aagaatggtt tccaaaagaa atcagcaaaa
    16741 gctatcccat caaaatttct aaacaataca caagcagtca acgttgggag atctatgcaa
    16801 aattaacgaa aaaacagcgg gaactagtcg ataagcaccg ccgatatcta atcagctctc
    16861 gttttatgga agaacattat ttagctgcaa cagattgggt gttttctgac ttcaagatca
    16921 atccgttttt tcgaacaaaa cgaagccagc aaaaattgta ttgtgagtgt ggtagagaat
    16981 taaaagtgca atacatcgtt aaatcgccaa aaaccggaaa gatcctaaaa ctaggcatca
    17041 accactttgc cgatcacctg catgtctctc cgacagtggc agcttcgatc catcagggaa
    17101 tgacaaaagt tgatctggcc ttagacgaat tattatggct taagcagaag aatatcgatt
    17161 ttcctgaagg actatggcaa aaatactgtt ttgtcttgta ccaaaatcgt cggatgaaac
    17221 agccatattt accggatata aaattagctc agcggctcgc agaatttcgc caagtagaaa
    17281 tgccgattta catagcggat tatcaagcat tggaaaatga aatcaagaaa ataagtgaac
    17341 acatcaatgg gcagtcaaaa aagcgacaga taaaaaaaga actatttgac gactttgcag
    17401 aagaattagt aaaggatgta gaagaattct tgatcaacta tcgcgcattt ttacgtaaag
    17461 attggcaatc catcgtatat gaagaagtgc cagtacatcc taatgcttat tttgaaacat
    17521 ttatttccgt gttgagaaag acaaaacgtc aacgaacacc agaagtcact gctcagatgg
    17581 aatattttgc gaaaaaccaa cgattcatcc aaccaaaaat ctatctattt atttggaagc
    17641 aatattgtcg ctatggtttt acagaagggt tcttcgacag cattcctcgg atagtcagaa
    17701 atggtttttt aaaagtattg cgtaaagaac gagaagctat acaatctgct gataagaaag
    17761 accgtacagt ctcaaaagaa aaatggcaac tagtagtgaa agatatccaa tctggaaatg
    17821 tgcaagagac aatcgacaaa tggaaaggaa aacattatcg ttttactgaa gctcagaaac
    17881 aagcgttgga atattatcag aagttagaag aatcactgcg tttcaatgat gaggcaagaa
    17941 agtatctgaa ggaattacta taaaaaatag aggctgggac agaagcgttt aactccaaga
    18001 aataagaa
//
LOCUS       DAFEHS010000211        12216 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.guided.078, whole genome shotgun sequence.
ACCESSION   DAFEHS010000211
VERSION     DAFEHS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.guided.078"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            163..510
                     /locus_tag="LP327_002890"
     CDS             163..510
                     /locus_tag="LP327_002890"
                     /inference="COORDINATES: protein motif:HMM:NF024908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL1726922.1"
                     /translation="MTGVKKSYEEKLKIVEDYIANRLTYLEAAEKNHVSYSNIYSWVN
                     KYKKHGPIGLEDNRGRGKPTEIQTTEERLNAEVETLKARNKWLEMENDALKKRRKIAG
                     NHKSQGLDKKWNI"
     gene            498..1358
                     /locus_tag="LP327_002891"
     CDS             498..1358
                     /locus_tag="LP327_002891"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL1726923.1"
                     /translation="MEYLTIEELKHKYPVIHLCDILGIAKSSYYKWLKREPSETELKR
                     LKLMRAIKGIHEAFGGIYGYRRMTIFLNFFRRAKVNHKCVHRLMRIMGITAVIRRKRR
                     NYVPHKAVHVAENILNRDFHAERPMEKLLTDVTEFRLTNGTKRYLSAIYDLGSKKIVA
                     YKTSHRNDNPLVLDTLKQILGDVKPETTLIHSDRGSQYTSHAFNKMIKDHQIIHSMSR
                     VSKCIDNGPMEGFWGTLKVEMFNLDTFDRPAYLDRKIKAYIAFFNNERVTLDMGLAIP
                     TEEKILQMIA"
     gene            complement(1443..4367)
                     /locus_tag="LP327_002892"
     CDS             complement(1443..4367)
                     /locus_tag="LP327_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBL1726924.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            4513..5088
                     /locus_tag="LP327_002893"
     CDS             4513..5088
                     /locus_tag="LP327_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL1726925.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            5302..5997
                     /gene="vanR"
                     /locus_tag="LP327_002894"
     CDS             5302..5997
                     /gene="vanR"
                     /locus_tag="LP327_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBL1726926.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            5975..7129
                     /locus_tag="LP327_002895"
     CDS             5975..7129
                     /locus_tag="LP327_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBL1726927.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            7344..8312
                     /locus_tag="LP327_002896"
     CDS             7344..8312
                     /locus_tag="LP327_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL1726928.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            8305..9336
                     /gene="vanA"
                     /locus_tag="LP327_002897"
     CDS             8305..9336
                     /gene="vanA"
                     /locus_tag="LP327_002897"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL1726929.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            9342..9950
                     /locus_tag="LP327_002898"
     CDS             9342..9950
                     /locus_tag="LP327_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL1726930.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            10378..11289
                     /gene="vanY"
                     /locus_tag="LP327_002899"
     CDS             10378..11289
                     /gene="vanY"
                     /locus_tag="LP327_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBL1726931.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            11442..11927
                     /gene="vanZ"
                     /locus_tag="LP327_002900"
     CDS             11442..11927
                     /gene="vanZ"
                     /locus_tag="LP327_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL1726932.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT         3873 a         2241 c         2526 g         3576 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaacccg gtttttcata cttagccgct
       61 aaatataaca ttccatctaa aacaacggta aacaaatggg taattcgata tactgaagga
      121 aaagagaatg agacatattc tccgaaatct gaggtgtaca acatgacggg tgttaaaaaa
      181 tcatatgaag aaaaattaaa aatcgtagaa gattacatag ccaatcgcct gacttatctg
      241 gaagcagcgg agaaaaacca tgtaagttac agtaacattt attcctgggt gaataaatac
      301 aaaaagcatg gaccgatagg tctggaagac aaccggggcc gcgggaagcc cacggaaatt
      361 caaacaacgg aagaacgttt aaacgcagaa gtagagacac tcaaagcccg gaacaaatgg
      421 ctagaaatgg agaacgatgc gttaaaaaag cgccgaaaaa tcgctgggaa tcacaaatca
      481 caggggttag acaagaagtg gaatatttaa ctattgaaga attaaagcat aaatatcccg
      541 ttatccatct ttgtgacata ctgggtatcg ccaaatccag ctactataag tggttaaaac
      601 gggaaccctc agaaacagaa ttaaaacgcc tgaaactgat gcgggcgatc aaaggaatcc
      661 acgaggcatt cggtgggatt tacggctacc gaagaatgac catctttctc aactttttta
      721 gaagagcgaa agtgaatcat aagtgtgtac accgcctcat gagaatcatg gggatcacag
      781 ccgtcatccg tcgcaaaaga aggaactacg tgccacacaa agccgtacat gtggctgaaa
      841 acatcttaaa ccgtgatttc cacgctgaaa gacccatgga aaagttattg acggatgtca
      901 cggaattccg gttgaccaat gggacaaaac gttacctgag cgctatttat gacctcgggt
      961 caaagaaaat cgtggcttat aaaaccagtc accgcaatga caacccgtta gtactggata
     1021 cactaaagca gattttgggt gatgtaaagc ctgaaaccac actgattcat agcgaccgcg
     1081 gttcccagta cacctcccat gcctttaaca agatgattaa agatcaccag ataatccata
     1141 gtatgtcacg cgtctcgaaa tgtattgaca acggccctat ggaaggcttt tggggtaccc
     1201 tcaaggtgga gatgtttaac ctggatacgt ttgacagacc agcgtactta gaccggaaaa
     1261 tcaaggcata catcgcgttc ttcaacaatg aacgcgttac tttggatatg ggattagcaa
     1321 ttcctacgga agagaagatt ctacaaatga ttgcataaac gctacaaaaa aagaccacac
     1381 atttttacat gcatggtcta aaatactttg tttttttacc tgtctacttg acagggggca
     1441 gttcactctg agttaaaatg gtattctcct agtaaattaa tatgttccca acctaagggc
     1501 gacatatggt gtaacaaatc ttcattaaag ctacctgtcc gttttttata ttcaactgct
     1561 gttgttaggt ggagagtatt ccaaatactt atagcattga taattatgtt taaagcactg
     1621 gctctttgca attgatgctg tatggtgcgt tctctaagct caccttgttt tccgaagaaa
     1681 atagctcttg ccaatccatt catggcttct cctttattca atcctctttg tatttttctt
     1741 cttaatgatt catccgatat ataattcaaa ataaagatcg ttttttctat tcggcccatc
     1801 tcacgtaagg ctgtagctaa gctgttttgt cttgaatagg aacctagctt ccccataata
     1861 agggatgctg aaactgttcc ctcccttata gaatgagcta atcgcaaaac atcctcataa
     1921 ttttctttaa tgacctttgt atttatttgt ccacgtaaaa tggcttctag ttttggatac
     1981 tcacttgctt tatctatcgt aaataatttt gagtccgata aatcccttat tcttggggca
     2041 aatttaaatc ctaataaatg agtcagtccg aatatttggt cagtgtaacc ggcagtgtct
     2101 gtataatgtt cctctatgtt tagatccgtc tcatgatgta acaaaccatc caaaacatga
     2161 atcgcatctc ttgaattagt atgaataatc tttgtgtagt aagaagagaa ttgatcactt
     2221 gtaaatcggt agatggtggc tccttttcca gttccataat gtggatttgc atctgcatgt
     2281 agtgatgaaa cacctagctg cattctcata ccatctgacg aagatgttgt accgtcgccc
     2341 caatagaaag gcaattgtaa tttatgatga aagtttacta atatggcttg ggctttattc
     2401 atggcatctt catacatgcg ccattgagat acattggcta gttgcttata tgtaagtccg
     2461 ggtgtggctt cggccatctt gctcaagcca atattcattc ccattcctaa aagggcagcc
     2521 atgataatga ttgtttcttc cttatctggt tttcgattat tggaagcatg agtgaattgc
     2581 tcatgaaatc ctgttatatg ggccacatcc atgagtaaat cagttaattt tattcttggt
     2641 agcatctgat aaaggcttgc actaaatttt tttgcttctt ctggaacatc tttttctaag
     2701 cgtgcaagtg atagctttcc tttttcaaga gaaaccccat ctaacttatt ggaattggca
     2761 gctaaccact ttaacctttc attaaagctg ctggttctct ccgttatata atcttcgaat
     2821 gataaactaa ctgataatct cgtattcccc ttcgattgat tccatgtatc ttccgaaaac
     2881 aaatattcct caaaatccct atattgtctg ctgccaacaa tggaaacatc tcctgcccga
     2941 acatgctccc gaagttctgt taaaacagcc atttcatagt aatgacgatt aattgttgta
     3001 ccatcatcct cgtataaatg tcttttccat cgttttgaaa taaaatccac aggtgagtca
     3061 tcaggcactt ttcgctttcc agattcgttc attcctcgga taatctcaac agcttgtaaa
     3121 agtggctcat ttgcctttgt agaatgaaat tccaatactc ttaatagcgt tggcgtatat
     3181 tttcttagtg aataaaaccg tttttgcagt aagtctaaat aatcatagtc ggcaggacgt
     3241 gcaagttcct gagcctcttc tactgaagag acaaaggtat tccattcaat aaccgattct
     3301 aaaaccttaa aaacgtctaa tttttcctct cttgctttaa ttaatgcttg tccgatgttc
     3361 gtaaagtgta taactttctc atttagcttt ttaccgtttt gtttctggat ttcctcttga
     3421 gccttacgac cttttgataa caaactaagt atttgcctat catgaatttc aaacgcttta
     3481 tccgttagct cctgagtaag ttgtaataaa tagatggtta atatcgaata acgtttattt
     3541 tcttgaaagt cacggaatgc atacggctcg tatcttgagc ctaagcgaga cagctgcaac
     3601 aggcggttac ggtgcaaatg actaatttgc actgtttcta aatccattcc tcgtatgtat
     3661 tcgagtcgtt ctattatttt tagaaaagtt tcgggtgaag gatgacccgg tggctctttt
     3721 aaccaaccca atatcgtttt attggattcg gatggatgct gcgaggtaat aatcccttca
     3781 agcttttctt tttgctcatt tgttagagat ttactaaccg tattaaatag cttcttttca
     3841 gccattgccc ttgcttccca caccattctt tcaagtgtag tgatagcagg cagtataatt
     3901 ttgttttttc ttagaaaatc tatgcattca tgcagtagat gaatggcatc accattttcc
     3961 aaagctaatt gatgaaggta cttaaatgtc attcgatatt cactcagggt aaaagttaca
     4021 aagtcgtatt cacttcgaat ttctttcaaa tgatcccaaa gtgtattttc cctttgagga
     4081 taatgatcaa gcgaggatgg actaacacca atctgtttcg atatatattg tatgaccgaa
     4141 tctgggatgc ttttgatatg agtgtatggc caaccgggat accgaagaac agctaattga
     4201 acagcaaatc ctaaacggtt ttcttccctc cttcgcttat taactatttc taaatcccgt
     4261 ttggaaaaag tgaagtaggt ccccagtatc cattcatctt cagggatttg cataaaagcc
     4321 tgtctctgtt ccggtgtaag caattctcta cctctcgcaa ttttcattca gtatcattcc
     4381 atttctgtat tttcaattta ttagttcaat tatatatcaa tagagtgtac tctattgata
     4441 caaatgtagt agactgataa aatcatagtt aagagcgtct cataagactt gtctcaaaaa
     4501 tgaggtgata ttttgcggaa aatcggttat attcgtgtca gttcgactaa ccagaatcct
     4561 tcaagacaat ttcagcagtt gaacgagatc ggaatggata ttatatatga agagaaagtt
     4621 tcaggagcaa caaaggatcg cgagcaactt caaaaagtgt tagacgattt acaggaagat
     4681 gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga tctatttgaa
     4741 ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga tacatggctt
     4801 gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc tggtgttaac
     4861 caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt ggctaagaaa
     4921 gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg aatgaattat
     4981 gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga aattactaat
     5041 gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc attctgtatt
     5101 ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta acagcctcct
     5161 agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa ttcttagtca
     5221 tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt aagggcaaac
     5281 ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga tgaacatgaa
     5341 attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt caaatactat
     5401 accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc catattggac
     5461 atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga caagcacacc
     5521 tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac agggttaaca
     5581 atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat tgctcgggta
     5641 aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt
     5701 atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct gaacgagaag
     5761 cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga aaacaagggg
     5821 aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata tttcagcaag
     5881 agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt
     5941 gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga aaaataaaaa
     6001 aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt
     6061 agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg gggattggat
     6121 cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga aattatatca
     6181 atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca ttagtattct
     6241 tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga taaataccgg
     6301 cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg aaatggatgt
     6361 tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc aggatgcaaa
     6421 gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc
     6481 ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca tgccggtaga
     6541 tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg aacagctaat
     6601 cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa caaaaacgca
     6661 catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc agctttccgc
     6721 acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg gcgaccctga
     6781 taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca gtgaggataa
     6841 cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa
     6901 cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct ataggctgga
     6961 caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg caaaagaaat
     7021 tattgttcag catggagggc agatttacgc ggaaagcaat gataactata cgacgtttag
     7081 ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag agatgtatat
     7141 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
     7201 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
     7261 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
     7321 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
     7381 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
     7441 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
     7501 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
     7561 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
     7621 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
     7681 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
     7741 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
     7801 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
     7861 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
     7921 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
     7981 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
     8041 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
     8101 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     8161 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     8221 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     8281 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     8341 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     8401 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     8461 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     8521 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     8581 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     8641 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     8701 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     8761 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     8821 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     8881 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     8941 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     9001 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     9061 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     9121 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     9181 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     9241 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     9301 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     9361 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     9421 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     9481 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     9541 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     9601 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     9661 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     9721 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     9781 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     9841 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     9901 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     9961 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
    10021 gttcttaggg aatttatata tagtagatag tattgaagat gtaaggcaga gcgatattgc
    10081 ggtcattatc tgcgtgcgct gcggcaagat agcctgataa taagactgat cgcatagagg
    10141 ggtggtattt cacaccgccc attgtcaaca ggcagttcag cctcgttaaa ttcagcatgg
    10201 gtatcactta tgaaaattca tctacattgg tgataatagt aaatccagta gggcgaaata
    10261 attgactgta atttacgggg caaaacggca caatctcaaa cgagattgtg ccgtttaagg
    10321 ggaagattct agaaatattt catacttcca actatatagt taaggaggag actgaaaatg
    10381 aagaagttgt tttttttatt gttattgtta ttcttaatat acttaggtta tgactacgtt
    10441 aatgaagcac tgttttctca ggaaaaagtc gaatttcaaa attatgatca aaatcccaaa
    10501 gaacatttag aaaatagtgg gacttctgaa aatacccaag agaaaacaat tacagaagaa
    10561 caggtttatc aaggaaatct gctattaatc aatagtaaat atcctgttcg ccaagaaagt
    10621 gtgaagtcag atatcgtgaa tttatctaaa catgacgaat taataaatgg atacgggttg
    10681 cttgatagta atatttatat gtcaaaagaa atagcacaaa aattttcaga gatggtcaat
    10741 gatgctgtaa agggtggcgt tagtcatttt attattaata gtggctatcg agactttgat
    10801 gagcaaagtg tgctttacca agaaatgggg gctgagtatg ccttaccagc aggttatagt
    10861 gagcataatt caggtttatc actagatgta ggatcaagct tgacgaaaat ggaacgagcc
    10921 cctgaaggaa agtggataga agaaaatgct tggaaatacg ggttcatttt acgttatcca
    10981 gaggacaaaa cagagttaac aggaattcaa tatgaactat ggcatattcg ctatgttggt
    11041 ttaccacata gtgcgattat gaaagaaaag aatttcgttc tcgaggaata tatggattac
    11101 ctaaaagaag aaaaaaccat ttctgttagt gtaaatgggg aaaaatatga gatcttttat
    11161 tatcctgtta ctaaaaatac caccattcat gtgccgacta atcttcgtta tgagatatca
    11221 ggaaacaata tagacggtgt aattgtgaca gtgtttcccg gatcaacaca tactaattca
    11281 aggaggtaag gatggcggaa tgaaaccaac gaaattaatg aacagcatta ttgtactagc
    11341 acttttgggg taacgttagc tttttaattt aaaacccacg ttaactagga cattgctata
    11401 ctaatgatac aacttaaaca aaagaattag aggaaattat attgggaaaa atattatcta
    11461 gaggattgct agctttatat ttagtgacac taatctggtt agtgttattc aaattacaat
    11521 acaatatttt atcagtattt aattatcatc aaagaagtct taacttgact ccatttactg
    11581 ctactgggaa tttcagagag atgatagata atgttataat ctttattcca tttggcttgc
    11641 ttttgaatgt caattttaaa gaaatcggat ttttacctaa gtttgctttt gtactggttt
    11701 taagtcttac ttttgaaata attcaattta tcttcgctat tggagcgaca gacataacag
    11761 atgtaattac aaatactgtt ggaggctttc ttggactgaa attatatggt ttaagcaata
    11821 agcatatgaa tcaaaaaaaa ttagacagag ttattatttt tgtaggtata cttttgctcg
    11881 tattattgct cgtttaccgt acccatttaa gaataaatta cgtgtaagat gtctaaatca
    11941 agcaatctga tctttcatac acataaagat attgaatgaa ttggattaga tggaaaacgg
    12001 gatgtgggga aactcgcccg taggtgtgaa gtgaggggaa aaccggtgat aaagtaaaaa
    12061 gcttacctaa cactatagta acaaagaaag cccaattatc aattttagtg ctgaggaatt
    12121 ggtctcttta ataaatttcc ttaacgttgt aaatccgcat tttcctgacg gtacccccaa
    12181 actaagattt catagataga ttttctgata acttta
//
LOCUS       DAFEHS010000212        10107 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.guided.089, whole genome shotgun sequence.
ACCESSION   DAFEHS010000212
VERSION     DAFEHS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.guided.089"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(268..1140)
                     /locus_tag="LP327_002901"
     CDS             complement(268..1140)
                     /locus_tag="LP327_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL1726933.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1268..1837
                     /locus_tag="LP327_002902"
     CDS             1268..1837
                     /locus_tag="LP327_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL1726934.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1904..2641
                     /locus_tag="LP327_002903"
     CDS             1904..2641
                     /locus_tag="LP327_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL1726935.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2872..4140
                     /locus_tag="LP327_002904"
     CDS             2872..4140
                     /locus_tag="LP327_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL1726936.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4627..5091
                     /locus_tag="LP327_002905"
     CDS             4627..5091
                     /locus_tag="LP327_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL1726937.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            5075..5704
                     /locus_tag="LP327_002906"
     CDS             5075..5704
                     /locus_tag="LP327_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL1726938.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            6058..7536
                     /gene="msr(C)"
                     /locus_tag="LP327_002907"
     CDS             6058..7536
                     /gene="msr(C)"
                     /locus_tag="LP327_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL1726939.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7583..8029
                     /locus_tag="LP327_002908"
     CDS             7583..8029
                     /locus_tag="LP327_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL1726940.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8228..8743)
                     /locus_tag="LP327_002909"
     CDS             complement(8228..8743)
                     /locus_tag="LP327_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL1726941.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8860..10061
                     /locus_tag="LP327_002910"
                     /pseudo
     CDS             8860..10061
                     /locus_tag="LP327_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         3220 a         1769 c         2094 g         3024 t
ORIGIN      
        1 gaaaaacctc ctagggaaac catgttcctc agagattttt catgcctagt atgatctagt
       61 cacatgaaac gaattattgt taacgtaatc aattttatat agacagttat gttttgtcaa
      121 ttcttttttt ataattatag taatttactg gaattatcag aaataaaagg aagaaataaa
      181 ttaagaaact tatgttattt aatcgttgga tcttttgctt atcttattcg ttgttaaaaa
      241 gctgaaagcg aaggaaaaaa actctactta actgtatttc tccgttagga catctaaaaa
      301 tttacgagca gcaggagaaa gaggctggtc ttttttccaa atgagattca gtgaagcttg
      361 taatggcgga tcgagtggga tgaatatttt ttccgtccca taagtattga tgatcccatc
      421 tagagctaat gcaccaaaag tcccgttatc gaccatcaaa gaagcattgt acaataagtt
      481 atatgttccg acgatcgata actgatctat gctgaaacct agccattcag ctagttgact
      541 gtccactgac gtttgccggg aaatgatcaa aggaacagtt ttcagttcct gcggatggat
      601 gacggtttgc tttgctaaag gatgatttcg atgtatcaaa agtccccaac gatcttgggc
      661 tggcagtcga aatgactggt atttttgttt gtttgtcggt tcgattacga gtccaaaatc
      721 aagtaatccg ctatccagtt tgtctatgat atcatctgca ttcccgctat ataaatgaaa
      781 ctggatatca ggatgcgcct tgatcaacga atgaaggcta tcagaaatga aagagattgc
      841 tttagtttcg cctccgccaa tgtagatttc tccagagatg attgcgtcat ttgttaaatt
      901 gttcgccgtt ttttctacca acgacaagat ttcttttcct tttgaataaa gatatcgacc
      961 agaatccgtc aactgtatct ctcgggagcc ccgagtgaaa agtgtcacac caagttcttc
     1021 ttctaaatct ttcagttgtt ttgagagagt aggttgcgtg atatgcagag attttgctgc
     1081 tcggctgata tttttttctc tagctactgc taaaaaatac gtcagtatac gaatatccat
     1141 tttttccctc ctgtttttaa aaaagcatac cactaagtat agttaaaagg aatagttaag
     1201 tatgttttat aagtattaga catgataagc cgtccgttat aaagtaagga taaaaggagg
     1261 gagcaaaatg acaagcctca tcgaaaaaca aatcgtcaat aaagagattc gagtaacgga
     1321 tccgctcttt gaagtgatcc atcagatcca agctgaaaac gaagagcctt tagcagcgct
     1381 gaatactggg taccatgaac cagcagatat ccgcaaacgg ctagaaaata tcattagtga
     1441 caaagtagat gatacggtga ctgttctgct gcctttttat acagatttcg gcaaacatat
     1501 tagtataggg aaaaatgtct ttatcaatcg tcaagtgatg ttcgttgact taggtggcat
     1561 ctgtttagaa gattcagtat tgattggtcc tcgtgtcaac ctcatcacgg tgaaccatct
     1621 aaccgatcca aaagaacgaa gaggcttgtc agtaaagcca atccacatca agaaaaatgc
     1681 ttggatcgga gcaggagcaa ctattctgcc aggcgtaacg attggcgaga atgccattgt
     1741 tgcagcgaat gcaactgtta cgaaagatgt cccagacaat accatcgttg caggaatccc
     1801 tgccaaaatc gtcaaacctg tcgaacggat tatttgaaca gtaagatgaa ataagagata
     1861 gtgagtataa gcaagagatt tttcaaaaca ggaggagaat agaatgaatg atttatcaga
     1921 aaaagtgatt gtcatcatgg gcgcttcaag cggaatcggt gaagcaacaa cgaagctatt
     1981 agcagaaaaa ggggctaaac tagttatagc tgcacgccga gaagatcgcc taaaagcaat
     2041 aaaagaatct ttaccagaag ctgagctata tattcaaaca gcagatgtaa gagattttgc
     2101 acaagtccaa gcagttatcg atttagcgat ggaaaagttc ggtcgaatcg atgtattgta
     2161 caataatgca ggaatcatgc caaccgctcc attagttgaa ggtcaccgtg atgaatggca
     2221 aaatatgctt gatatcaaca tcatgggtgt gttaaatgga atttccgccg tacttccaat
     2281 catggaaaaa caaaaatctg ggcatatcat ttcaactgat tctgtggcgg gacatgttgt
     2341 ttatcctgat tccgccgttt attgcggaac aaaatttgct gtccgtgcaa tcatggaagg
     2401 attacgtcaa gagcaacggc aaaataacat caaatctacg atcatctctc caggagctgt
     2461 tcaaactgaa ttatatcaaa ccatcagcga caaaaaggct gctcttgaac ttcatgaagc
     2521 gcaaaaagaa tggggattga cctccgaaga tatagcttct gctgtagcat ttgcgataga
     2581 aacgccagac cggatgtctg tcagtaacat gattatccgt ccgacgacac aggaagtata
     2641 atccataacg gataatatcc aaagagaatt acttgttaaa tagttattca aacgaccatc
     2701 aatttaaggc gtgaatggag aagaatattc ttcatttacg tctttttatg tagaaaaagc
     2761 aaatttatca aaaagaaaca aatgttattt aaatatggac attgacttgc ttttactttt
     2821 ttagttacac tgttatttag gaaaagtttc ataattcaaa ggagaaaaaa gatgaaagca
     2881 aagaatttat tatttagcgt tattctttta ggaaatctag cagttgccaa cacaacagtg
     2941 ctagctgaag aatcgacaga aactgtcgca actcagacat cgagttcttc agaaacagga
     3001 attgaaaacg atacagagag tactttgcag tcagctactg tctcagaaaa tacaatttca
     3061 accgaagaac caacaactga aacaacagaa caaacacaaa ccgaagacag tacatcagca
     3121 gaagctcaga acaatccaga gaatgcagaa gaaatcacga accaattgac agatgggcaa
     3181 acattgacac atacccatca agcagatttg tctgaattcg aaaactatac tggctatgct
     3241 ttttatgctc gctcatctgc taattctcaa caagcgttca ttgatagtat tgcctcaact
     3301 gcacaaagtt tggcaagtgc caatgactta tatgcatcag tcatgattgc tcaagccatc
     3361 gttgaaagtg gctgggggaa cagtactttg gcttctgccc ctaattacaa tctatttggg
     3421 attaaaggaa gctataatgg ccaatctgtt acgatgccta cttcggaata tgtgaatggg
     3481 caatggatta cggtgaatgc cgcttttaga aaatatcctt cgtacaaaga atcattgcaa
     3541 gataacgtaa cggtattgaa gacgacttct tttcagccag gcgtttacta ctattccggt
     3601 gcatggaaaa gcaatacgaa ctcttacaaa gatgctacgg cttggctaac aggtcgttat
     3661 gccactgcgc ctaattatgg ttctacattg aacaatgtaa tcgaaaccta taatctgaca
     3721 cagtatgata cagcaccaac ttcaacttct ccgatgtatc gtctgtataa ccgtcataca
     3781 ggagaacatt tatatacatt gaatgccgga gaaaaaaatt atctgccaac tgtaggctgg
     3841 gaatatgaag ggattgcttg gcaagcacca aacagcggac aacctgtcta ccgtttatac
     3901 aatccttaca gtggagacca tcattacaca atggcacaat ctgagatcaa ctctttaaca
     3961 aaaatcggtt ggcgttatga aggtctaagt ttttattctg gtggttccaa gccaatttat
     4021 cgactattta atccaaatga aaaaactggt acccatcact ataccttgag tgctaaggaa
     4081 agagattggc taacaccgat gggctggaga tatgaaggaa taggatttta tggttattaa
     4141 aaagaggtag tgaaaaagct gccctgcatc aagtagtaag aacagccaaa caaaaacaga
     4201 ttttcatgtt ttttgtttgg ttgaacttat taccgcagat gcaagttttt gaacaccgtt
     4261 tactcaaaaa aggttgtgac aacatcttgt cataactttt ttattactgt ttaagcatac
     4321 tgatccaatt tctaatattc gacgggattc ttgcaaaagg cgatttcgga aatttttggc
     4381 ggtgtaaaat tttctactgc agaagagcta tcttctatag tttgataaga atcaataatc
     4441 ttggttttag cgttgtgctt tctttatcat tttttccgtt cattctacaa aaaatttctt
     4501 ttctctatac ttttctattg agagcgtttt ttttattaca cagctgattc tttgactttt
     4561 ttcttttttt attctattat tgacaagtca atcgttgacg agtcaattat tagaaaggag
     4621 gaaattatgt ctttagaaga gattagtcac cttttatatc aaatcaaatt agccgatcaa
     4681 aaaatcgtcc agatctttga agaaaatatc ggtataagtc ttactagata cgagatcatg
     4741 atgagactga aagaagaaaa tccattgcta caaagcagtt tgcaagaaaa gttgcagatt
     4801 gatcaaggag cagttactcg tcatctgaag gtattggaag caaatggcta tgtttcgcgt
     4861 cagagaaatc ctcagaacaa ccgggaagtg tatgtagcgc taacagaaaa agcccagaag
     4921 aaaatcgagc attgtgaaat ggaccataaa gatacaggtt ccgtgctatc cccggctttc
     4981 agcgatgtag aaatgaaaca atttctaaca cttttagata aattgaatac ccaactttgc
     5041 aatataacaa cagaaacgaa agaggttaat aataatggaa caaaaaaatg atttttcaac
     5101 aattatgact ggtagaagat ccgttcgcgt atatgatgaa acatacaaaa tcccacacga
     5161 agaaatgctg gaaatgatca aagaagcgac gactgcacct tcttctgtca atatgcaacc
     5221 atggcgcttt gtggtcgctg aaagcgagga agcaaaagaa atcttgcgtc ctttgatccg
     5281 tttcaatacg agacaaaatg atacttcttc tgcaatggtc atgatttttg gcgatatgca
     5341 atgttacgaa tatggcgaag aaatctataa ccaagcttac gaatcaggga agatgccaaa
     5401 agaagtcaga gatcaacaat tagctgcgat cattccttat tacaagagtt tttcaagaga
     5461 agaaatgaac gatgtagtca aagtcgattc tagcttagcg gctatgcaat tcatgttagt
     5521 tgcgcgtgat catggctatg aaacaaatcc aattggtgga ttcgaagcag atcagttggc
     5581 agaggcattt ggtttggata aagaccgtta tgttccagtt attattttat caataggaaa
     5641 agcggtagaa gaaggttacg agtctgttcg tttagcacca gaaaagatca ctacgttcga
     5701 ataaaaggtt ttcagttact attcagtagc tattgctttt tttgaatcgt gtgtggtaac
     5761 ctaataaaaa aggaggtcgg aaaaatgact gcatcgatga aattacgttt cgaacttttg
     5821 aataacaact aaataaattc aaaagacatg tataaacaac aatttcatgg atggagtatg
     5881 tccatttttt cgaacgacag tagaataggt gaaagctatg acagatttgg tgatttagtc
     5941 atagttattt cgttcagagt aggaaaagat gtgatgcagt ttttttgcaa acagattttt
     6001 ctgctctttt ttagtatcga taattgttgt tttacatgaa ataggagtgt aaaacatatg
     6061 gaaaatttag cagtaaatat aacaaatcta caagtcagct ttggcaacca gctagaacta
     6121 tctattgatt ctcttcgtgt ctatcagcaa gatcgtatag ggatcatagg agaaaatggt
     6181 gtcggtaaat cgactttgct caaactaata gccggtgaac tttttcccga tcatgggaaa
     6241 atccaaacag agatcacctt caactacctg cctcaattaa cctatctcgc tgaagcaaag
     6301 gacctaaatt tggaattagc cagtcatttc cagttaagac tggaagaaac ttcagagcgg
     6361 aaatggagcg gaggggaaga acgaaagatc gagttgatac gtcttctttc ttcttatgaa
     6421 caagggatgc ttctagacga gccaacaacc catctagata gaaaaagtat tgatcgactg
     6481 attgaagagc ttcgttatta ttatggtacg ctggtttttg ttagtcatga tcgctatttt
     6541 ctagatgagc tagcatcgaa aatctgggaa gtaaaagacg gagaaatccg agagttttcg
     6601 gggaattata gtgcctatct cactcaaaag gaattggaga aaaagactca gctacgagaa
     6661 gcagagtcga tcatgaaaga gaaaaaacga ttggaaaaat cgatccaaga aaagaaaaaa
     6721 caagcggaaa agttggaaaa agtgtccagt aaaaagaaaa agcaacaaat cagaccggat
     6781 cggttgtctt cctctaaaca aaaagacagt gtacaaaaag ccatccaaaa gaatgcgaaa
     6841 acattagaga gaagactcca aaaaatagga gaaacaacca agccgcagca gatgaaacaa
     6901 atccgttttc cagtaccaaa atctcttgaa ctccacagcc gctatcccat catgggacaa
     6961 aatgtccaat tggaaagaag tgggagaaca ttactggtaa atggtgattt tcagttttct
     7021 ttaggtaaaa aaatcgcgat tgtcggtgaa aatggttcag gtaagacaac cttattagaa
     7081 catatccgca aacaaggaga aggaatcctt ctctctccga aagtaagctt tcaagtatat
     7141 cagcaaaagg gttatcaaat gacatctgaa gaatccatca ttcgttttgt catgagacaa
     7201 acagagtttt cagaatcgct tgtccgtagt ttgctgaatc acttagggtt tgctcaggaa
     7261 actttgacga aaccgttatg tacattaagt gggggagaag cgacccgtct gacgattgct
     7321 ttgcttttta ctaagccaag taatgtgttg ctgttagatg aaccgactaa ttttattgat
     7381 atggcaacga tcgaagcttt agagaagcta atgcaaatat atccgggaac gattttgttt
     7441 acttctcatg attcttactt tgtcgagcgt acggctgatg aagtttatga aataaaaggg
     7501 cagaaaataa aaaaagtact tacgagaaat ttttaaatgt gctacgatga ttttcgtgac
     7561 aaatttaaag gagtggtaca gattgcctac aaataaaaaa gaaggaataa tttttacaac
     7621 aatgatgtgt tttttgatgg tcttggggat gagcgcgtat aatttatggc tgcatcaaga
     7681 tcttcattta tcaagtttgt tgattggtct agtgcctgga ttcgtggtgg ctttcatttt
     7741 ggatgtcttc atcatcggtg tgctggcgaa aaaaattgcc tttaaactac cagtaaatcc
     7801 agaaagcaag gtccaaatga tcttaactat ttcatgcttg atggttttag gaatggtgac
     7861 atgtatgtct ttattcggtg tgatcatgga acaaggaata cctgatcaat tattctctgc
     7921 ttatatgaat gcttggaaaa tgaatgtgat tgccgcttta ccgttgcagt tgatcatcgt
     7981 tggcccgctt tcaagaagtg tgctaaaagc aattcaaagt aatgaatgat ggaacactgt
     8041 tatttgaaaa aaggccttgg catgcttttt gtcatagtct cttgaatgca gttctagaaa
     8101 aatagtgatc cgaacgatag gttttggcct gtcgttcgga tcgctttttc ttttccaact
     8161 gtattgctct tttattgaag ataatttgct cttcaataga tatttctaga aaggttctgt
     8221 ttatccctta ctcaaaaaac tgctgcattt gttctcttac ttgctgacac cattggagtt
     8281 cctgctgaca aagcataatt ctgcgctgga tcacaacata acgtccaaat tcttgtttct
     8341 tgtctagcga gaggtcattc aatttttttt gatttttttg catgattttg ttcagctgtt
     8401 gttctctagt gaatagcagt ccagtagctg ttgttttatc gagcgtcgaa aaagcataga
     8461 tttttgctag aaattcatct ttttgcgtag gagcgggagt ttcctctttg atccattcct
     8521 ctacaagagc acgtccttca tcagtcaagt ggtagatctt tttttgagag tgctcgtctt
     8581 tgtccaaaac ggtcacataa ccctgctggt ctaacttggc aagcgttgta tagatctgac
     8641 tgtgatgggc ttcccaaaaa attgtgatca gctgcttcaa ttcataccca gaacgtggtt
     8701 tggataaaag cacgcataat aatatatagg ctaaagtatt cataaaaatc ctacctctcg
     8761 tcttattttc tttattatat cacagtaaag tttccacgtt ttacatgtgc atatttacat
     8821 gtaaaataaa tcatgtatga tgctaataga gagaaaggga tggaaatatt ggagaatcaa
     8881 atagatatac aagaacaaac aaatgaagaa acaaagggga aaagttcagc gctagttgta
     8941 gcagctacct gtatgatcgc ttttatggga gtgggtctag tagacccaat cttaaaaacg
     9001 attgctgcac agttaaacgc tacgccagct gaaacgaccc tgttatttac tagttacatg
     9061 ttagtcactg gtgtggtgat gctatttaca ggtttcttat ctagtcggat cggtttgaaa
     9121 aaacattgat gacaggattg tttatcatcg ttgtattcgc tggtcttggc ggattatctg
     9181 ggaatatcgg aatgctgatt gggttacgtg ccggctgggg tgtcgggaat gctttatttg
     9241 tctctactgc tttagcaacg atcgtcagta ttttagttgg taacacagaa aaagcaatca
     9301 tgatgtatga agcagcactt ggctgcggaa tggctgttgg ccctctagtc ggtggggtct
     9361 tgggcagtgg ttcatggcgt tatccatttt ttggggtagc atttttgatg ttttgcggtt
     9421 ttatagccct agcggtactt ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt
     9481 gggaaggtgt caaagcgtta agcaatcata aacttcgttc agttggacta atcgcattgt
     9541 tatataattt cggattcttt actttacttg cgtatgcgcc atttttattg attggctatt
     9601 ctgaattaga agtcggtttt gtattttttg gctggggtgt gcttctggct attgcttcga
     9661 ttttcgttgc accggctctt gagcggaagt ttacgactta tcggacaatt ttagcagcat
     9721 ttgtcttatt cattatttgt ttgctgttat taggagctgg aacttctatg ccagtccttg
     9781 taccgatcag cattgtgtta gcaggatttt tccaaggaat tatcaataca ctgttgacga
     9841 cgattgctat ggaaatacca ggtctgcaac gaaacgttgc ttcgtccagc tatagttttg
     9901 tccgcttttt cggtggagct ttagcaccat ttattgcagg tgtgattggt gaacatattg
     9961 gtgaaagcta tgcgttttat tttgcaggtg ccattgttct tatcagtgta tgttgtatca
    10021 tttatcaccg taattatttt gctacaaatg aagaagaata agaaatcaga ggttgtgaaa
    10081 aagctgtcct gcatcaagtc ataagaa
//
LOCUS       DAFEHS010000213         9670 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.guided.092, whole genome shotgun sequence.
ACCESSION   DAFEHS010000213
VERSION     DAFEHS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.guided.092"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            complement(565..792)
                     /locus_tag="LP327_002911"
     CDS             complement(565..792)
                     /locus_tag="LP327_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL1726942.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(801..1274)
                     /locus_tag="LP327_002912"
     CDS             complement(801..1274)
                     /locus_tag="LP327_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBL1726943.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            complement(1914..2087)
                     /locus_tag="LP327_002913"
     CDS             complement(1914..2087)
                     /locus_tag="LP327_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL1726944.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            2504..3070
                     /gene="amaP"
                     /locus_tag="LP327_002914"
     CDS             2504..3070
                     /gene="amaP"
                     /locus_tag="LP327_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL1726945.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3083..3271
                     /locus_tag="LP327_002915"
     CDS             3083..3271
                     /locus_tag="LP327_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL1726946.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3284..3841
                     /locus_tag="LP327_002916"
     CDS             3284..3841
                     /locus_tag="LP327_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL1726947.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3873..4115
                     /locus_tag="LP327_002917"
     CDS             3873..4115
                     /locus_tag="LP327_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL1726948.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4456..5669
                     /locus_tag="LP327_002918"
                     /pseudo
     CDS             4456..5669
                     /locus_tag="LP327_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
     gene            5700..5918
                     /locus_tag="LP327_002919"
                     /pseudo
     CDS             5700..5918
                     /locus_tag="LP327_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6337..6810
                     /gene="dfrF"
                     /locus_tag="LP327_002920"
     CDS             6337..6810
                     /gene="dfrF"
                     /locus_tag="LP327_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL1726949.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            6937..7593
                     /locus_tag="LP327_002921"
     CDS             6937..7593
                     /locus_tag="LP327_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL1726950.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            7775..8305
                     /locus_tag="LP327_002922"
     CDS             7775..8305
                     /locus_tag="LP327_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL1726951.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            8851..9093
                     /locus_tag="LP327_002923"
     CDS             8851..9093
                     /locus_tag="LP327_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL1726952.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            9090..9560
                     /locus_tag="LP327_002924"
     CDS             9090..9560
                     /locus_tag="LP327_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL1726953.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWGKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
BASE COUNT         3402 a         1406 c         1890 g         2972 t
ORIGIN      
        1 ctcgagttac gagtttggaa cagcttttgt ctacagtctg tcattattat caaaataatg
       61 ttaaaaataa aaaagaattg tccatcactg ttattcaaat cattttatca aaactacata
      121 gtaaatgtta aaaagcaaca tgacataata tatagaaggc tttacagtat atatgggagc
      181 ttactaaaaa agaatggagt aagagttaac acaatgggcc attagctgaa gttttaagca
      241 cttcaaggta tgaaacagaa tggcctgtaa tactacatga ttatttagac tgctcaaact
      301 gctatcagtc tggtggaatg aataaagtat tatactaaaa gcagatccaa ctacaacgta
      361 acaggaaacc agttgtatca ggaaaagtga tagtacatgg agaagaaaag gccccctttg
      421 tatgagtcag tacatatctt atttatagat aaaacggtat tcaatcgaaa taaaaaatct
      481 gaataataga cgtaaataac ttctaaaagt ctcacacatc tatcattcag attatttttt
      541 atttactgtt tgttctactt tattttattc ttctggtata gtattcgagt catttttata
      601 ggtcaacaaa tcacctggtt gacactccaa aacttggcag attttttcga gagtggaaaa
      661 acggatggct tttgcttttc cattttttag aatagagatg tttgccatcg taatgcccac
      721 tttctcactc aattcagtca cactcatttt tttttgagct aatactacat ctaaattaac
      781 gataattgcc atgtcgcacc tcacactgtc aaatcatgat ctgtcttaag taaaatcgca
      841 tgttcaagca gtttttccag cacgcttgtt aatgtgtata ctgcaaaagg aatgaatacg
      901 acagcgattc ctaccaacat caaacctgga gcatcgtcta aatccgctac tttcataaat
      961 ttaggaagta agccaaaaaa ggcgaaacca ataaacaaaa tggttcgttc gatttttttt
     1021 agtatgttta agctttgagt ggaaaaagct tggttatctt cgattttttt caatagtttc
     1081 aataagaaat aagcaataac gatccctagg agaagggcaa agtaaatgac agaaacaaat
     1141 aatgtggtct gccaatcaaa attgaatcct ttttcgcttg tagcgatctg agtataaaaa
     1201 agtacgccga acaaaataaa tacggcactt atacctaaaa cgacaaattt taatagcaat
     1261 gttttaaatt tcatttttat ccctctttta tctttgatag ctaaaatata gcagaataat
     1321 tattgtaaaa caactatttt ttatcgaaaa acaataaaaa tatatcgttt ataaaaaatg
     1381 atagagtatg aaggtgtgga gtgcatttaa cagctttttt tgattgtaag aaacatttaa
     1441 aaccagttat tttagattct atgtgtgtct cttctataat gctagtttag atgcctggga
     1501 ttatattgga caatacagtt ggaggatttt ttattatcta gcgaagagaa gtgtaagtta
     1561 acagaactgt atgagaaaaa agacaactac gtagtagcta accgattcat taattttttc
     1621 aagttcatgt ttatttacgt atcagaattt ttcttattca atggtttggt gtaatcacca
     1681 cttatttgtc ctttattgct tatacaatgt tatacaaaag gaaaatcaag cattgacgca
     1741 tggggtggta ctatctagct gaaatagctt aataagcgca agtagatgca ttggattagt
     1801 ttataaatca aaagtgttag aggtattaag aagaagtaga aaaataaaat ggattgccga
     1861 ataaaaagta ccataaatcc taaaacatgt gccaacagat agatggcaca tgtttatttt
     1921 tttcgataat tttgtaacaa aaatacgaag atgaaaaaga atagagattc ccataatgta
     1981 gtagtccaac tgaattcttt actgatacca aataccatat ttcctagatt tgatacaatc
     2041 aaatagccaa tcgttatgat caatgaatag tacaatcttt tcatcatagg acatacctcg
     2101 tttttattta taagaatagt ataaaccaaa gaagtgaata agaaaaatga taatataaaa
     2161 ataaagtaag aacgcattaa agtaactaaa gttatttatt gtatttttta attctccgaa
     2221 aaaatttaat aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt
     2281 aatcttgaat aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa
     2341 gcaattgtta tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg
     2401 gtagcgcttt atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt
     2461 agagttattt acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa
     2521 aataatcggt gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat
     2581 caatgcgccg tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa
     2641 ttactacatg caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat
     2701 catcttgcta gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga
     2761 agatggcaaa ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca
     2821 cgaagaagag ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca
     2881 tatccatgtc aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt
     2941 gatgcaagat atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt
     3001 ggcagtcaca tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg
     3061 agtggaatag gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat
     3121 ctttgggggt cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac
     3181 attgttgatc ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc
     3241 aggaatagtt gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat
     3301 gaatacaaca aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg
     3361 tgaattgact tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt
     3421 agatggtcta ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa
     3481 cacagataac gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga
     3541 tctagatgtg atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga
     3601 attgatcagt actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa
     3661 tgttgcagac atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa
     3721 agtaacagaa gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa
     3781 atctgctgta ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata
     3841 agaagtaaga agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg
     3901 gcggtatcat tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca
     3961 tcgcaaacat cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt
     4021 ggggaccaac aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag
     4081 tattagtcgt ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg
     4141 aaatgaagaa caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt
     4201 tatatgaagt attctttatt ccttatgaaa aaatatcttt aaaaataaaa aaagacgaaa
     4261 ataaacggtt ttctataaaa agaagaccgt ttattttttg tctttatttg tgtgaaaaac
     4321 gaaaaagagc acaacacatc aagcttttat taattgtctg tcttttataa atgcaagtat
     4381 taattcaaaa aaacagaaga aaaaaacaac tacaccttct tttatactcg taatcgtaga
     4441 aaggaggcga aaaaaatgaa tacattacct atcaatacag gagatacagc attcatgatt
     4501 ttatgtacag caatggtttg cctgatgact cctggtctag ctttttttta cgcggattag
     4561 cacggaagaa taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa
     4621 cgattatgtg ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta
     4681 ttggaaaccc ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc
     4741 atggtgcaac gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta
     4801 ccgttccttt gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac
     4861 ttgttatttt ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg
     4921 gatttttgga tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag
     4981 ctggattcgg ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag
     5041 aaagaagtca gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg
     5101 gctggttcgg ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat
     5161 ttggttcaac attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag
     5221 taaaaggaaa tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag
     5281 ccattacgcc ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg
     5341 cgggtatcgt ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac
     5401 ttgatgtgtg gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct
     5461 ttgcatctaa cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag
     5521 tgattggtgt catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat
     5581 cggcgatgca ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact
     5641 ttttcttctt tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca
     5701 tgcaacaaag caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta
     5761 tgaccatctc aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca
     5821 gcttagtatt tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg
     5881 cagcagagat ggcaacggtg aaaggatggg aaaaaggtgt ttcccaaaca aaaagatatt
     5941 aagaaacagc ccatgtttac ctgcttgcaa acaaaagaat attgctacta gactaagaga
     6001 tactacataa gtgtgttggt atctcttttt tgttgccgtg aaacagcaga ctatttcatt
     6061 tctgcttgat taccttaaaa cttattagtc tatggtttgt caagggcttg ttgcgtgaga
     6121 aatgctgaat atcacccttt acaaaacata ggctgataag taacttttca aaagcagaaa
     6181 agaaatggca atgcttataa caatatgata taatgcagat gaagacaaaa aaggaatttg
     6241 tcgagctggt tagttcgaga taattttgag tttgttaaga tgtggtgcac ttgatggaat
     6301 cacaggtacg acatcatatt atggaggatg aaatagatga taggtttgat tgttgcgagg
     6361 tcaaagaata atgttatagg caagaatggt aatataccat ggaaaataaa gggagaacaa
     6421 aagcaattta gagagttaac aacgggtaat gtggttatta tggggcgaaa gtcttatgaa
     6481 gaaatcggtc atccgttgcc taatagaatg aatattgttg tttccaccac aacagagtat
     6541 caaggagata atttagtttc agttaaatca ttagaagatg cattattatt ggctaaagga
     6601 cgagatgtat acatatctgg tggatatgga ctatttaagg aagctttgca aatagtagat
     6661 aaaatgtata tcacagaagt agatttaaat attgaagatg gagatacatt ctttccagaa
     6721 tttgatatca atgattttga agttttgata ggggaaacac ttggtgagga agtgaaatat
     6781 acgagaacat tttatgtaag gaaaaaatga attgagtaaa ttttggattt agggtgtttt
     6841 cataaatata ttgcttttta tgcatatata aattgttgta agacaaattc cagtttgtat
     6901 ggggcgataa aatcagaatt tgagagagga ttgaatatga ataaggaatg gtctgaactt
     6961 aataaaacaa tgcaggcaca aataaaaaag aaagatactt ataagagggg tattgatact
     7021 ttacttactt tacgaagtca gttaatacaa accttagtat ctttcaaaga agaattatgc
     7081 agagaagatt ttaactcaat ccccttcata aacgctgatg gttatcatag taagacgatt
     7141 gcttattcta tttggcatat tttccgcatt gaagatatag ttgtgcatac agtgataaat
     7201 gaagatgaac aagtattttt tgcaggcaat tatcaagagc gtatcaattc acctatcatt
     7261 acaacaggaa atgaactcat gaaacagcag attgcagact tttcaaaaca gcttaatctg
     7321 gaagaattat atttatatat tttcgaagta tgggaaagca ctgagaaaat gcttgaacgg
     7381 ttatcttatg atgaattgaa aagaaaaata ccgaaagaaa gaaaaggata cttagaatcg
     7441 ttgaatgtag taaacgacaa tgaaaaagca atttggctga ttgattattg gtgtaataaa
     7501 gatatttgtg gactaattca aatgccgttt tcaagacatt ggattatgca cacagaagcc
     7561 tgtttgcgta taaagaacaa gatacattca taggcaaaaa tttcagtttg tagaactaag
     7621 gaaattggaa tttaaggagg aataaagtat ggttagtgat tttataagag cagcatttcc
     7681 atggatttta atgggtttat ttgtagcaat cagttgttcc cttatgagta aaaaagagaa
     7741 ataaattcct gtttgtattt ataaaagaga aaaaatgaga aaagtagttt tatttattgc
     7801 catgagcctt gacggatata ttgcggacgg taatggtgga gtagcttggc taaatggtca
     7861 tggaaatgac aatgaaaaca tcgacactta tacagaattt accaaagata tagatactgt
     7921 cttaatgggg tggaatactt ctcatcaggt tgttactgaa ctttctccgc aagaatgggt
     7981 atataataaa tttacaactt atgtattaac acataaagag tgcaattctt cccaacaaat
     8041 tcattttaca agtgaaaatc ctgttttatt attggaacgg ctaaaacaag aagcaggaaa
     8101 ggatatttgg atttgtggcg gagcaaatct tgttcagcag ttggtaagca aaaatatgat
     8161 tgataaatat tacatttctg ttattcctac tctgttagga aatggggttc gtcttttggg
     8221 caacatagac agggaaataa agttaaggct ttgtaaaaca cagacttata acggaataac
     8281 cgatttgatt tatatgcgta gataactaga gagaaacaga aaagctgtta aaccgacgga
     8341 gcgatccgac cacgttggcg ggaaaatcct taagattttt ccgccatgct tgcaaggggg
     8401 cggttcgctt cggcggttcg gcgagcttgc gagccgttca aagcccccgt tatctaacaa
     8461 ggggggcaca aaaaacaaga aacactatac actaacaagc ggaaaacgct gtatttataa
     8521 ggcaaaaccc catttttaca gtagtgatat aaattgtacc tatatcacta tgaaaattga
     8581 atagtaaata tgagaaagac agttgatttc aaaaaataag aaattgattg tcgttttctt
     8641 ttgcagaaat ttaagtgcag gcaattatgg tagttgtcgt aattgctgtc agctcagtgt
     8701 ttatcactga tggtgtgaaa gatttacggt tggagatgtt caataagatt attggtgctt
     8761 aattattttt attgtagtgc tgatatgaga atgttataat tggggtataa aacatacaaa
     8821 taaatttgct gaaaaatgag gtatcaaaat atggaaaatg aattactgga aaacttagga
     8881 agacttcaca caactgaact tggcgttatt agaattaaaa agaacttatc tttgaatgta
     8941 gaaaatgtaa ttgaatggtg caaagaaaaa attagccttt ctaatgcgga aatcacaaga
     9001 aaaggaaaaa actggtatat aacgattgat aattgtatta taacggtaaa tgcgtacagt
     9061 tataccgtta tcactgctca caaggtaaaa tgataaattt ttctataaag gaattaaacg
     9121 catttgcagt tattgggcag gaagtagaac tgactaatta tcaaaaaaag aatattcaga
     9181 ttagtacaca gttttgggga aagtttaaca gtagtttgaa aaaagcgtat ctttcacaat
     9241 caggaaattg ggtaaaatat gcttttatgg aaagaagaaa tggaaaactt tattatttct
     9301 gttctattcc ccaaagaacc attaccccag agggctttct gtataaagaa ataccgtctt
     9361 ataaatattt ggttgtggag catattggtg ctatggataa aatatatgaa acttatggca
     9421 agatttatga agaaattatt cctagtacat cgtatattcc tataaaagat attatcctac
     9481 actttgaaaa atacgattat cgttttcatt ggaatagtga taattcagtt attgaaattt
     9541 ggatacctat tcaagattag aagcaatttt atatccatac acacaaagca gttagtctta
     9601 ggattgactg ctttttctat acagattttt aaggctcttt gtcaataagg actgatgagt
     9661 tgtgcaaaat
//
LOCUS       DAFEHS010000214         6376 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.guided.115, whole genome shotgun sequence.
ACCESSION   DAFEHS010000214
VERSION     DAFEHS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.guided.115"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="LP327_002925"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="LP327_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL1726954.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="LP327_002926"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="LP327_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBL1726955.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3976..5238
                     /locus_tag="LP327_002927"
     CDS             3976..5238
                     /locus_tag="LP327_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL1726956.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5483..6370
                     /locus_tag="LP327_002928"
     CDS             5483..6370
                     /locus_tag="LP327_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBL1726957.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKWFCCKVLNKE"
BASE COUNT         2210 a          906 c         1313 g         1947 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga
     4021 ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt
     4081 gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac
     4141 gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat
     4201 gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat
     4261 ccaggagaac aaaaacgatt ttttgaggaa agttataaat tattttccga acgatatggc
     4321 aagcaaaata ttgcttatgc aacagttcat aatgatgagc aaacccctca catgcattta
     4381 ggtgttgtgc ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa tcgtcaagaa
     4441 ctgttatggc tacaagataa attccccgag catatgaaaa aacagggttt tgagttgaag
     4501 cgtggtgaac gtggctctga ccgtaaacat attgagacag ctaaatttaa aaaacaaact
     4561 ttggaaaaag agattgattt tctagaaaaa aatttagcag ttaaaaaaga tgaatggact
     4621 gcttatagcg ataaagttaa atcagattta gaagtaccag cgaaacgaca catgaaaagt
     4681 gttgaagtgc caacgggtga aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca
     4741 gaaaagaaac caaccaaaaa tgttgttata tcggagcgtg attataaaaa cttagtgact
     4801 gctgcgagag ataacgatag gttaaaacag catgttagaa atctcatgag tactgatatg
     4861 gcgagagaat ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa atatagtggt
     4921 cttgtagagc gatttaatga aaatgtaaat gattataatg agttgcttga agaaaacaag
     4981 tctttaaagt ctaaaataag cgatttaaag cgtgatgtga gtttaatcta tgaaagcact
     5041 aaggaattcc ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt ttttaagggg
     5101 tttgtagaca aggtaaagga taaaacagca caattccaag aaaaacacga tttagaacct
     5161 aaaaagaacg aatttgaact aactcataac cgagaggtaa aaaaagaacg aagtcgagat
     5221 cagggaatga gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt ttgatggttt
     5281 tgaacttgtt ctttcttatc ttgatatata tagaaataac gtcattttta ttttagttgc
     5341 tgaaaggtgc gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa cccttaaaat
     5401 tggttgcaca gaaaaacccc atctgttaaa gttataagtg accaaacaaa taactaaata
     5461 gatgggggtt tcttttaata ttatgtgtcc taatagtagc atttattcag atgaaaaatc
     5521 aagggtttta gtggacaaga caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat
     5581 cgctaatgtt gattactttg aacttctgca tattcttgaa tttaaaaagg ctgaaagagt
     5641 aaaagattgt gctgaaatat tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt
     5701 gtatcgagtg tggttttgta aatccaggct ttgtccaatg tgcaactgga ggagagcaat
     5761 gaaacatggc attcagtcac aaaaggttgt tgctgaagtt attaaacaaa agccaacagt
     5821 tcgttggttg tttctcacat taacagttaa aaatgtttat gatggcgaag aattaaataa
     5881 gagtttgtca gatatggctc aaagatttcg ccgaatgatg caatataaaa aaattaataa
     5941 aaatcttgtt ggttttatgc gtgcaacgga agtgacaata aataataaag ataattctta
     6001 taatcagcac atgcatgtat tggtatgtgt ggaaccaact tattttaaga atacagaaaa
     6061 ctacgtgaat caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat tagactatga
     6121 tccaaatgta aaagttcaaa tgattcgacc gaaaaataaa tataaatcgg atatacaatc
     6181 ggcaattgac gaaactgcaa aatatcctgt aaaggatacg gattttatga ccgatgatga
     6241 agaaaagaat ttgaaacgtt tgtctgattt ggaggaaggt ttacaccgta aaaggttaat
     6301 ctcctatggt ggtttgttaa aagaaataca taaaaaatgg ttctgttgca aagttttaaa
     6361 taaagaataa aatccc
//
LOCUS       DAFEHS010000215         3814 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.guided.135, whole genome shotgun sequence.
ACCESSION   DAFEHS010000215
VERSION     DAFEHS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.guided.135"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            183..1250
                     /gene="rlmN"
                     /locus_tag="LP327_002929"
     CDS             183..1250
                     /gene="rlmN"
                     /locus_tag="LP327_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL1726958.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1443..2546)
                     /locus_tag="LP327_002930"
     CDS             complement(1443..2546)
                     /locus_tag="LP327_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL1726959.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3099..3647
                     /gene="aac(6')"
                     /locus_tag="LP327_002931"
     CDS             3099..3647
                     /gene="aac(6')"
                     /locus_tag="LP327_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL1726960.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1247 a          645 c          767 g         1155 t
ORIGIN      
        1 gatagaatga ttttctatca gtcctagaaa ttatagagcc cttttctaat acggtatgat
       61 gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg
      121 cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
      181 ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag
      241 aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
      301 taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc
      361 attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa
      421 aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat
      481 atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg
      541 caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca
      601 tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg
      661 taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca
      721 tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat
      781 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct
      841 tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa
      901 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
      961 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
     1021 atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca
     1081 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
     1141 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
     1201 cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta
     1261 ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta
     1321 aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc
     1381 atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt
     1441 atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg
     1501 tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca
     1561 tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg
     1621 tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa
     1681 tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga
     1741 aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca
     1801 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
     1861 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
     1921 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
     1981 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
     2041 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
     2101 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
     2161 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
     2221 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
     2281 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
     2341 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
     2401 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
     2461 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
     2521 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
     2581 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
     2641 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
     2701 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
     2761 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
     2821 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
     2881 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
     2941 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
     3001 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
     3061 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
     3121 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
     3181 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
     3241 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
     3301 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
     3361 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
     3421 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
     3481 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
     3541 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
     3601 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
     3661 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
     3721 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
     3781 tgtgaaaaag tcgcttagtt tcgagtagta agaa
//
LOCUS       DAFEHS010000216         1183 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.guided.185, whole genome shotgun sequence.
ACCESSION   DAFEHS010000216
VERSION     DAFEHS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.guided.185"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            109..192
                     /locus_tag="LP327_002932"
     CDS             109..192
                     /locus_tag="LP327_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL1726961.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="LP327_002933"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="LP327_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL1726962.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1059..>1183
                     /locus_tag="LP327_002934"
     CDS             1059..>1183
                     /locus_tag="LP327_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBL1726963.1"
                     /translation="MSRFFKFGKLHVTKGNGDKLLDILLTASKKLKRSLAPTGNL"
BASE COUNT          443 a          189 c          191 g          360 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gta
//
LOCUS       DAFEHS010000217         1049 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.guided.189, whole genome shotgun sequence.
ACCESSION   DAFEHS010000217
VERSION     DAFEHS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.guided.189"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            49..579
                     /gene="sat4"
                     /locus_tag="LP327_002935"
     CDS             49..579
                     /gene="sat4"
                     /locus_tag="LP327_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL1726964.1"
                     /translation="MKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEAPYLK
                     SYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDIAVCK
                     DFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDTMLYA
                     NFENNFEKAVFWYLRF"
     gene            672..1010
                     /locus_tag="LP327_002936"
                     /pseudo
     CDS             672..1010
                     /locus_tag="LP327_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3') family aminoglycoside
                     O-phosphotransferase"
BASE COUNT          375 a          164 c          244 g          266 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccga ttacagaaat gaaagcaggg
       61 cacctgaaag atatcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg
      121 aggtatgaaa acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa
      181 agctaccaag acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac
      241 aatactgata agataatata tctttactac caagacgata aatgcgtcgg aaaagttaaa
      301 ctgcgaaaaa attggaaccg gtacgcttat atagaagata tcgccgtatg taaggatttc
      361 agggggcaag gcataggcag cgcgcttatc aatatatcta tagaatgggc aaagcataaa
      421 aacttgcatg gactaatgct tgaaacccag gacaataacc ttatagcttg taaattctat
      481 cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac
      541 aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg
      601 gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa
      661 ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
      721 accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
      781 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
      841 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
      901 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
      961 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ggttctgttg
     1021 caaagtttta aatctactat caaataagg
//
LOCUS       DAFEHS010000218          838 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.guided.196, whole genome shotgun sequence.
ACCESSION   DAFEHS010000218
VERSION     DAFEHS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.guided.196"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            31..732
                     /locus_tag="LP327_002937"
                     /pseudo
     CDS             31..732
                     /locus_tag="LP327_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
BASE COUNT          355 a           61 c          138 g          284 t
ORIGIN      
        1 tgaggttgaa cttttacaca agattgtgga cttgactcaa atttagaaac taatgtaaaa
       61 attcctaata ttgaatattc gtatattagt gatgaattat ctatactagg ttataaagaa
      121 attaaaggaa cttttttaac accagaaatt tattctacta tgtcagaaga agaacaaaat
      181 ttgttaaaac gagatattgc cagtttttta agacaaatgc acggtttaga ttatacagat
      241 attagtgaat gtactattga taataaacaa aatgtattag aagagtatat attgttgcgt
      301 gaaactattt ataatgattt aactgatata gaaaaagatt atatagaaag ttttatggaa
      361 agactaaatg caacaacagt ttttgagggt aaaaagtgtt tatgccataa tgattttagt
      421 tgtaatcatc tattgttaga tggcaataat agattaactg gaataattga ttttggagat
      481 tctggaatta tagatgaata ttgtgatttt atatacttac ttgaagatag tgaagaagaa
      541 ataggaacaa attttggaga agatatatta agaatgtatg gaaatataga tattgagaaa
      601 gcaaaagaat atcaagatat agttgaagaa tattatccta ttgaaactat tgtttatgga
      661 attaaaaata ttaaacagga atttatcgaa aatggtagaa aagaaattta taaaaggact
      721 tataaagatt gattatataa tatatgaaaa gctattataa aagacattag tattaaatag
      781 tttaaaaaaa tgaaaaataa tggttctgtt gcaaagtttt aaatctacta tcaaataa
//
LOCUS       DAFEHS010000219          700 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR39047_1
            SAMN20572274-rid13343433.guided.202, whole genome shotgun sequence.
ACCESSION   DAFEHS010000219
VERSION     DAFEHS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572274
            Sequence Read Archive: SRR16917407
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001177130.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001177130.1
            Genome Coverage       :: 577x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 09:07:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,938
            CDSs (total)                      :: 2,885
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 34 of 77
            Pseudo Genes (incomplete)         :: 33 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 8 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572274-rid13343433.guided.202"
                     /isolate="PR39047_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2019-07"
                     /collected_by="University of Michigan"
     gene            1..664
                     /locus_tag="LP327_002938"
                     /pseudo
     CDS             1..664
                     /locus_tag="LP327_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          292 a           64 c          118 g          226 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agattacgtc catataattg tgtaaaagtg aagcatcaaa
//
